LOCUS       DATRME010000001        94969 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRME010000001 DATRME010000000
VERSION     DATRME010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 94969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94969
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(440..2050)
                     /gene="ftsY"
                     /locus_tag="VBC23_000001"
     CDS             complement(440..2050)
                     /gene="ftsY"
                     /locus_tag="VBC23_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6841988.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
     gene            complement(2050..2874)
                     /locus_tag="VBC23_000002"
     CDS             complement(2050..2874)
                     /locus_tag="VBC23_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6841989.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            complement(2874..3671)
                     /locus_tag="VBC23_000003"
     CDS             complement(2874..3671)
                     /locus_tag="VBC23_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6841990.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            complement(3762..7301)
                     /gene="smc"
                     /locus_tag="VBC23_000004"
     CDS             complement(3762..7301)
                     /gene="smc"
                     /locus_tag="VBC23_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6841991.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(7309..7995)
                     /locus_tag="VBC23_000005"
     CDS             complement(7309..7995)
                     /locus_tag="VBC23_000005"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6841992.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            complement(8170..8538)
                     /locus_tag="VBC23_000006"
     CDS             complement(8170..8538)
                     /locus_tag="VBC23_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6841993.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            complement(8541..9350)
                     /locus_tag="VBC23_000007"
     CDS             complement(8541..9350)
                     /locus_tag="VBC23_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6841994.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            complement(9354..10703)
                     /gene="vicK"
                     /locus_tag="VBC23_000008"
     CDS             complement(9354..10703)
                     /gene="vicK"
                     /locus_tag="VBC23_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6841995.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            complement(10696..11406)
                     /locus_tag="VBC23_000009"
     CDS             complement(10696..11406)
                     /locus_tag="VBC23_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6841996.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            11746..12501
                     /locus_tag="VBC23_000010"
     CDS             11746..12501
                     /locus_tag="VBC23_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6841997.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            12513..13313
                     /locus_tag="VBC23_000011"
     CDS             12513..13313
                     /locus_tag="VBC23_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6841998.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            13326..14021
                     /locus_tag="VBC23_000012"
     CDS             13326..14021
                     /locus_tag="VBC23_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6841999.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            14033..14683
                     /locus_tag="VBC23_000013"
     CDS             14033..14683
                     /locus_tag="VBC23_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6842000.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(14901..16094)
                     /locus_tag="VBC23_000014"
     CDS             complement(14901..16094)
                     /locus_tag="VBC23_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6842001.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(16173..16379)
                     /locus_tag="VBC23_000015"
     CDS             complement(16173..16379)
                     /locus_tag="VBC23_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6842002.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16372..17514)
                     /locus_tag="VBC23_000016"
     CDS             complement(16372..17514)
                     /locus_tag="VBC23_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6842003.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            18026..18661
                     /locus_tag="VBC23_000017"
     CDS             18026..18661
                     /locus_tag="VBC23_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842004.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18815..18892
                     /locus_tag="VBC23_000018"
                     /pseudo
     CDS             18815..18892
                     /locus_tag="VBC23_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            19137..19253
                     /locus_tag="VBC23_000019"
     CDS             19137..19253
                     /locus_tag="VBC23_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6842005.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19269..21188
                     /gene="tet(M)"
                     /locus_tag="VBC23_000020"
     CDS             19269..21188
                     /gene="tet(M)"
                     /locus_tag="VBC23_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6842006.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21307..21474
                     /locus_tag="VBC23_000021"
     CDS             21307..21474
                     /locus_tag="VBC23_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6842007.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21534..21887)
                     /locus_tag="VBC23_000022"
     CDS             complement(21534..21887)
                     /locus_tag="VBC23_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842008.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22392..22715
                     /locus_tag="VBC23_000023"
     CDS             22392..22715
                     /locus_tag="VBC23_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6842009.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            22694..23023
                     /locus_tag="VBC23_000024"
     CDS             22694..23023
                     /locus_tag="VBC23_000024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842010.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            23250..23669
                     /locus_tag="VBC23_000025"
     CDS             23250..23669
                     /locus_tag="VBC23_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6842011.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            23674..23913
                     /locus_tag="VBC23_000026"
     CDS             23674..23913
                     /locus_tag="VBC23_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842012.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(24098..24664)
                     /locus_tag="VBC23_000027"
     CDS             complement(24098..24664)
                     /locus_tag="VBC23_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6842013.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(24831..25850)
                     /locus_tag="VBC23_000028"
     CDS             complement(24831..25850)
                     /locus_tag="VBC23_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6842014.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            complement(25948..26652)
                     /locus_tag="VBC23_000029"
     CDS             complement(25948..26652)
                     /locus_tag="VBC23_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842015.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            complement(26873..28816)
                     /gene="thrS"
                     /locus_tag="VBC23_000030"
     CDS             complement(26873..28816)
                     /gene="thrS"
                     /locus_tag="VBC23_000030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6842016.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            complement(29273..30607)
                     /locus_tag="VBC23_000031"
     CDS             complement(29273..30607)
                     /locus_tag="VBC23_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6842017.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            complement(30609..31607)
                     /locus_tag="VBC23_000032"
     CDS             complement(30609..31607)
                     /locus_tag="VBC23_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6842018.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            complement(31652..33118)
                     /locus_tag="VBC23_000033"
     CDS             complement(31652..33118)
                     /locus_tag="VBC23_000033"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6842019.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            complement(33250..34254)
                     /gene="ccpA"
                     /locus_tag="VBC23_000034"
     CDS             complement(33250..34254)
                     /gene="ccpA"
                     /locus_tag="VBC23_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6842020.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            34464..35549
                     /locus_tag="VBC23_000035"
     CDS             34464..35549
                     /locus_tag="VBC23_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6842021.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            complement(35607..37397)
                     /locus_tag="VBC23_000036"
     CDS             complement(35607..37397)
                     /locus_tag="VBC23_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6842022.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(37413..38225)
                     /locus_tag="VBC23_000037"
     CDS             complement(37413..38225)
                     /locus_tag="VBC23_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6842023.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            complement(38378..39217)
                     /locus_tag="VBC23_000038"
     CDS             complement(38378..39217)
                     /locus_tag="VBC23_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6842024.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            complement(39341..40387)
                     /gene="uxuA"
                     /locus_tag="VBC23_000039"
     CDS             complement(39341..40387)
                     /gene="uxuA"
                     /locus_tag="VBC23_000039"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6842025.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            complement(40405..41805)
                     /gene="uxaC"
                     /locus_tag="VBC23_000040"
     CDS             complement(40405..41805)
                     /gene="uxaC"
                     /locus_tag="VBC23_000040"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6842026.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            complement(41822..42439)
                     /gene="eda"
                     /locus_tag="VBC23_000041"
     CDS             complement(41822..42439)
                     /gene="eda"
                     /locus_tag="VBC23_000041"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6842027.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGSGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            complement(42556..43227)
                     /locus_tag="VBC23_000042"
     CDS             complement(42556..43227)
                     /locus_tag="VBC23_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6842028.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            complement(43256..45055)
                     /gene="uidA"
                     /locus_tag="VBC23_000043"
     CDS             complement(43256..45055)
                     /gene="uidA"
                     /locus_tag="VBC23_000043"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6842029.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            complement(45072..46097)
                     /locus_tag="VBC23_000044"
     CDS             complement(45072..46097)
                     /locus_tag="VBC23_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6842030.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            complement(46164..47714)
                     /locus_tag="VBC23_000045"
     CDS             complement(46164..47714)
                     /locus_tag="VBC23_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO6842031.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            complement(47921..48913)
                     /locus_tag="VBC23_000046"
     CDS             complement(47921..48913)
                     /locus_tag="VBC23_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6842032.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            complement(48927..49985)
                     /locus_tag="VBC23_000047"
     CDS             complement(48927..49985)
                     /locus_tag="VBC23_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6842033.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            50178..51062
                     /locus_tag="VBC23_000048"
     CDS             50178..51062
                     /locus_tag="VBC23_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6842034.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRSLGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            complement(51099..51917)
                     /locus_tag="VBC23_000049"
     CDS             complement(51099..51917)
                     /locus_tag="VBC23_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6842035.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(52081..52854)
                     /gene="fetB"
                     /locus_tag="VBC23_000050"
     CDS             complement(52081..52854)
                     /gene="fetB"
                     /locus_tag="VBC23_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6842036.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(52851..53507)
                     /locus_tag="VBC23_000051"
     CDS             complement(52851..53507)
                     /locus_tag="VBC23_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6842037.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            53724..54362
                     /locus_tag="VBC23_000052"
     CDS             53724..54362
                     /locus_tag="VBC23_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6842038.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(54440..55225)
                     /locus_tag="VBC23_000053"
     CDS             complement(54440..55225)
                     /locus_tag="VBC23_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6842039.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            55486..56931
                     /locus_tag="VBC23_000054"
     CDS             55486..56931
                     /locus_tag="VBC23_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6842040.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(56988..57659)
                     /locus_tag="VBC23_000055"
     CDS             complement(56988..57659)
                     /locus_tag="VBC23_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842041.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(57756..58076)
                     /locus_tag="VBC23_000056"
     CDS             complement(57756..58076)
                     /locus_tag="VBC23_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6842042.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(58085..58213)
                     /locus_tag="VBC23_000057"
     CDS             complement(58085..58213)
                     /locus_tag="VBC23_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6842043.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            complement(58314..59108)
                     /locus_tag="VBC23_000058"
     CDS             complement(58314..59108)
                     /locus_tag="VBC23_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842044.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(59118..60347)
                     /locus_tag="VBC23_000059"
     CDS             complement(59118..60347)
                     /locus_tag="VBC23_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842045.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            complement(60471..61532)
                     /locus_tag="VBC23_000060"
     CDS             complement(60471..61532)
                     /locus_tag="VBC23_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6842046.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            complement(61567..62586)
                     /locus_tag="VBC23_000061"
     CDS             complement(61567..62586)
                     /locus_tag="VBC23_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6842047.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDEDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            complement(62621..63652)
                     /locus_tag="VBC23_000062"
     CDS             complement(62621..63652)
                     /locus_tag="VBC23_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6842048.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            complement(63791..64609)
                     /locus_tag="VBC23_000063"
     CDS             complement(63791..64609)
                     /locus_tag="VBC23_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6842049.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            complement(64630..65715)
                     /locus_tag="VBC23_000064"
     CDS             complement(64630..65715)
                     /locus_tag="VBC23_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6842050.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            complement(65899..69216)
                     /locus_tag="VBC23_000065"
     CDS             complement(65899..69216)
                     /locus_tag="VBC23_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842051.1"
                     /translation="MNNKGVGGDGVQIYQYYIKMDNNKPYLSPKDKTTVEKLEDRWKK
                     ITFKVQDTGIGLKDVYLQSVKYVGGGNNNLDLITPPGFKKEDKKVEKPKLDRPPGIDL
                     PAPTSMRSFDYSTPPGTKPSKPKDSLSTPPGFPDLNTPPDEAPKDSKKDAIEDKSGAI
                     KYAKSLQLSFVDDPILASKVNGKILQVESDGKLVIPRNALSANQFDDTSLKIYRNNNR
                     NKEITITTDYFADTKYVNITAVDYLSNTTFEQLATGETVDYHAIVFSSFAAIKDKGGK
                     IYVNDKLQETSRIALKDKSVKIGIELPNDVRHIDSLSVRRLNEVKTVDNILKNDEQDI
                     NLSKTYQLKYNPTNRRLEFTINNINSSSEIMTTFKDGKMPELVEQKDVSLDINDMDMS
                     KFKTIRLGRKDSEFKGQLIAKTGTVELDMFFKQSQDPASIIKKIYLIQNGVPNELKKF
                     DSSFGLTESQIDGYYIYKDAINLKFKLTSGASLKVVYKGQEDPYSHQKEDMTKKGEQL
                     SHSTQANENTAKVTFANIDWSHYSKVTVNGKEVGKGSELPLTKGWTTFVLHKTENSLN
                     VKSLIMETGSVSKKVQQLPLSPRLSKNKHMRDMLLTMQKDSAYYETSDSLVLRINLTA
                     DTKLNFNAVKGASALTENMMMRQFAVAGPQDDPVSEHKYPSVFLLTPALLETASEATL
                     NGKEITASGIIGHIKDGDKSKHVEVKMVNENGDMLGTPVIIQGKDLTNRTKPLMSGRR
                     VLYAGKQYEFRAKLPLSRFNTWIRVEVVTEAGEKASIVRRMFFDQSVPELNTAVAKRD
                     LTSDTALIHIVAKDDSLKLKLYQDDSLLESVDKTGLYSFRNGVEITKDMTVPLEFGDN
                     IIKLSAVDLSNYRRNETLHIYRNRFDVKASQMTADKGAKVTVDMLMKHLVVPEMAGAY
                     TLTIDEAPNTNESGMLTNAKVSIHYVNGGVDKVDVPIKVVDLEAIRKAEEAHKADEAR
                     KAEEARKAEEAHKAEEVRKAEEAHKVEEARKAEEGHKTQEAPIVEEGYKVNNVHQTDT
                     TVKASDLPKTKTVSAVHMARTDNKQITSHQTHVEKQIKNTLPSTGDSKRGYYITGMAI
                     VMLSVLFSLAKKFKSKY"
     gene            complement(69262..71919)
                     /locus_tag="VBC23_000066"
     CDS             complement(69262..71919)
                     /locus_tag="VBC23_000066"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6842052.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            complement(71971..72486)
                     /locus_tag="VBC23_000067"
     CDS             complement(71971..72486)
                     /locus_tag="VBC23_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842053.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            complement(72520..72861)
                     /locus_tag="VBC23_000068"
     CDS             complement(72520..72861)
                     /locus_tag="VBC23_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842054.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            complement(73204..73779)
                     /locus_tag="VBC23_000069"
     CDS             complement(73204..73779)
                     /locus_tag="VBC23_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6842055.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            complement(73748..74959)
                     /locus_tag="VBC23_000070"
     CDS             complement(73748..74959)
                     /locus_tag="VBC23_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6842056.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            complement(74964..77159)
                     /locus_tag="VBC23_000071"
     CDS             complement(74964..77159)
                     /locus_tag="VBC23_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6842057.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            complement(77156..78109)
                     /locus_tag="VBC23_000072"
     CDS             complement(77156..78109)
                     /locus_tag="VBC23_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6842058.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            complement(78106..80109)
                     /locus_tag="VBC23_000073"
     CDS             complement(78106..80109)
                     /locus_tag="VBC23_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6842059.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            complement(80106..80984)
                     /locus_tag="VBC23_000074"
     CDS             complement(80106..80984)
                     /locus_tag="VBC23_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842060.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            complement(80977..81906)
                     /locus_tag="VBC23_000075"
     CDS             complement(80977..81906)
                     /locus_tag="VBC23_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842061.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            complement(81896..82372)
                     /gene="fabZ"
                     /locus_tag="VBC23_000076"
     CDS             complement(81896..82372)
                     /gene="fabZ"
                     /locus_tag="VBC23_000076"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6842062.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            complement(82356..82661)
                     /locus_tag="VBC23_000077"
     CDS             complement(82356..82661)
                     /locus_tag="VBC23_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6842063.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            complement(82654..83376)
                     /gene="fabG"
                     /locus_tag="VBC23_000078"
     CDS             complement(82654..83376)
                     /gene="fabG"
                     /locus_tag="VBC23_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6842064.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            complement(83373..84221)
                     /locus_tag="VBC23_000079"
     CDS             complement(83373..84221)
                     /locus_tag="VBC23_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6842065.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            complement(84221..84526)
                     /locus_tag="VBC23_000080"
     CDS             complement(84221..84526)
                     /locus_tag="VBC23_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6842066.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            complement(85151..85936)
                     /locus_tag="VBC23_000081"
     CDS             complement(85151..85936)
                     /locus_tag="VBC23_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842067.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQRIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            complement(85938..86756)
                     /locus_tag="VBC23_000082"
     CDS             complement(85938..86756)
                     /locus_tag="VBC23_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6842068.1"
                     /translation="MNRIWRRYKPFISTEIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            complement(86759..87751)
                     /locus_tag="VBC23_000083"
     CDS             complement(86759..87751)
                     /locus_tag="VBC23_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6842069.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRNEL"
     gene            complement(87951..89102)
                     /locus_tag="VBC23_000084"
     CDS             complement(87951..89102)
                     /locus_tag="VBC23_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6842070.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            complement(89163..89432)
                     /locus_tag="VBC23_000085"
     CDS             complement(89163..89432)
                     /locus_tag="VBC23_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842071.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            complement(90777..90953)
                     /locus_tag="VBC23_000086"
     CDS             complement(90777..90953)
                     /locus_tag="VBC23_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842072.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            complement(92051..92656)
                     /locus_tag="VBC23_000087"
     CDS             complement(92051..92656)
                     /locus_tag="VBC23_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6842073.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            complement(92905..93695)
                     /locus_tag="VBC23_000088"
                     /pseudo
     CDS             complement(92905..93695)
                     /locus_tag="VBC23_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(93749..>94969)
                     /locus_tag="VBC23_000089"
     CDS             complement(93749..>94969)
                     /locus_tag="VBC23_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEO6842074.1"
                     /translation="LYWRDYNWVYPFDPKTKKVSATKQIKTHGEPTTLYFNGNIRPKG
                     YDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNVDDTNKIYDELNKYFKTIVEE
                     KHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGNDGSQLKNGVALGGPNSDGGIL
                     KDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDNYISNKFYNTNNRTTLSPKSE
                     KEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIKVNKDKHSESLLGAKFQLQIE
                     KDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYEISSPDGYIEVKTKPVVTFTI
                     QNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPPGVFPKTGGIGTIVYILVGST
                     FMILTICSFRRKQL"
BASE COUNT        29594 a        18520 c        14831 g        32024 t
ORIGIN      
        1 acaagtgaag taggattact tcgattagtt gttcacccct tcgttccatt tccattacag
       61 aaacttcatc actactatgg gttcggctga cttcttgtcg ttcgttgtta ctaggtttta
      121 cccttgacag gacctcacgg gataagtcgg acatctttcc tcgtttactc tcgtgattta
      181 cgtatatagg ttacgactac ctatttggac tttagagttt atagccccct catccgctat
      241 atacgccttc atatcacgtt tctgttcgta gagccatgat ttcgctatcc cttcctctcc
      301 ccgctacctc acgatagtca agcttgggag tcgctattgg gttcaccggt agcgggtgcc
      361 cacagaggac ttgcacctca gatgtacgac atgcccgtcg tactataaaa agaactttct
      421 aaatgaaagt tcttttttct tataaaattc cttccagaag gccacgcatg aaatcttctg
      481 aattaaattc accaatatca tcgatttttt cgccgaaacc aatgaattta acaggaatat
      541 ctagttcttg acgaatagcc agtaccacac cacccttagc agtaccatca attttagtca
      601 gaataaggcc agttaatggt gtaattttcg agaactcttt agcttgactt aaagcatttt
      661 gtcctgtaga tgcatctaaa gcaagcaaag tttcgtgggg agcatctggt acgacacgct
      721 taataatacg tccaattttt tccaactctg ccataaggtt ttctttattt tgcaaacgtc
      781 ctgctgtatc aattaacaag acatcaacgc cttgagcaac ggctttttcc atgccatcaa
      841 agacaacgct agccggatct gctttttttt ctccagtgac aactggtaca tcaacacggc
      901 gtccccattc tactagttga gccacagcac ctgcacggaa agtatcagct gctactaaca
      961 taactttttt cccttgagac ttgtattgat gagctaactt acctatagat gtcgtttttc
     1021 caacaccatt aacacctaca aaaagcatta cggttaaacc ttcttggaaa ttgatagcct
     1081 cattataaat accatctttt tcgtagattt caactaattt ttcaacgata acacgtttta
     1141 aatcctctga tttcttggca ttctctaatt ttgcttcata acgaaggtct tcagttaact
     1201 gtgttgcaac atttacccca acgtcagata agattaacat ttcttctaat tcttcaaaga
     1261 actcttcgtc aacacgacgg aagttagaaa ggaaagcatt aaggcgtgca ctaaaacctg
     1321 tgcgtgtttt tttcaggcta cgattatatt tttcctcttg actctcagct tttatagtgt
     1381 ctgcctgtga ttgcgataaa acttcctgtg cctcagaaag ttctgactca tctgttgaca
     1441 gcgaattaga cgagatttct ttctcaattg ctttacgttt agcatagtaa tctgctaaaa
     1501 aagcatcagc cgcagatgta tcctctgact ctgtttgtac ctcagttttt ccactagaca
     1561 tctcttcttg aaggggagtt tctgaaaact gctctgaagc ctgactatca cttgattgct
     1621 cctcatcact aaaagatgcc tcagatttag cagaaacatt gtcagaaaga gtcgtggaat
     1681 caggaactac atttcctacc tcagaagtat ctgccacctt tgtcttagcc ctatcttcta
     1741 gttctaaaaa actatcgtct tcttttacag gaatttcaga ggtatcttct gactctaaag
     1801 gatgaatttc agaggtaact ggttgatgtg attctgacaa tgctgtatct ctttctaatt
     1861 ctaagaaaga atcatcttct gcgacaggga cttcagaggt tcgattcaaa gttgactcct
     1921 tcgaaaagtt gctgctttcc tctttatcaa taactgaatc ttcattttct aaaacaacag
     1981 attctgatgc ttcaatttct agttctttat cttttttctt atgtccaaat aggcggtcaa
     2041 ataatcccat taattttcct cacttaaaat atatttccca actgcttcgg caactccaga
     2101 ctcatcgtta ttacaaatag ttgtcgcttt tgctgttgcc ttagcttctg gaataccatt
     2161 agccattgca actcctaagc ctgcccactc tagcattgat aaatcatttg cttcatctcc
     2221 cattgccata acttgactac tatctaaccc aaggtgtttt gtcagtagtt ctaagccgac
     2281 tgctttatga acacctttag gcatcaattc taaaatgata tcacgtgatt taaacatttc
     2341 atagtcaact ttaagtctat caggaagttt agcaatttgt tgatcaagat atgtagcatc
     2401 aataacacta acaattttat tataaactac atcctttggc acttgctcta aatcatcaac
     2461 ttctataaat tctaagagtg ggttggctaa atgatattga gaatggtgtc ccttgttagc
     2521 aatactatat accgttccct cacttaagat atctgttggc aaacccactt gatacaactc
     2581 ctcatggata tcttccactt cttgtcgtgt cattgcagtt ttagctaaga ttttccctgt
     2641 attttgttga actaaaccac cgttaaaagt aatgctgtaa tcttcatcag atactaactc
     2701 taggtcctct aacaaattac caattgcttt taaaggacgc cctgttgtaa taacaacttt
     2761 tatacctttt tctctagctg cttttaaggc cactttattt tcttctgaaa ctttcttatc
     2821 tgttgtaaat aaagtgccat ctaaatctaa ggcaagtatt ttaatatcca acattaatcc
     2881 accatacttt ctaaataagc cattacagct tgatcattgt gatgacctat aatacaatct
     2941 gctatctctt taacttctgg tctagcattt tctgttgcga tagcttttcc tgaccactct
     3001 agcatttcta aatcattaat attgtcacca aaagataaga cttcttctgc tctaataccg
     3061 tattgttcac agaggtgctc taaaccattt cgtttattaa ctgagcttag aataatatca
     3121 atggatttaa aacctgttgt cactgctgtg gcatacggta tagcttgatt tacccactcc
     3181 tcagcttgtc taacagtctc ttcagtaaag ttagccgtta ctttgaaaac aatatcatca
     3241 acatcttcaa agtgagaaac tttttgcaaa ttatcataat aatgagtgat gaattcaata
     3301 taatctggat tggcatcact tagaatatat gcaccatcct tgccagataa gacatattca
     3361 ttattctcca tataagggct cttactcaaa tggtcaataa tatctaagta ttgttctcga
     3421 ctcaagtgct gttcatatgc caagcgatta aataagactg ctgcacttcc gttttctgct
     3481 ataaaagcca tctgatcacg aaagtcagca aataattgtt caagtgataa taaggaacgc
     3541 ccacttgctg ccgtaaagac aattccttgt tctttaaatt tttttagcac atttgctaaa
     3601 cgttttttat cataggtacc gttttcatct aagaatgtac cgtccatatc ggttgctact
     3661 aattttatca tggaatctcc taactaaagg gtttacattt actattgtaa cataaaataa
     3721 ggctgaaaat ccaaaaaaca gaagggatcc ccttctgttt tctaattggt catttcttga
     3781 gcttctttta attttacaga aacaattttg gaaacgcctg attcctgcat cgttacccca
     3841 tagatactat cagcagcaga catagtccct tttcggtgag taacaacgat aaattgactt
     3901 gatttatcaa atcgattaag ataatcgcca aatcgcttaa catttgcttc atctaaagcg
     3961 gcctcaactt catccaagat gacaaatgga attgtcttaa cacggataat ggcaaataat
     4021 aatgctaacg ctgataatgc tttttcacca cctgacatca agtttaaaga ctggattttt
     4081 ttaccaggag gttgaaccga aatatcaacc ccagctgata ataaatcacc ttctgtcaga
     4141 ataaggttcg cagaacctcc accaaacatc tgtacaaagg tttctttgaa actatgacga
     4201 atggcttcaa atgtcgattt aaaacgagtc ttaacttcat catccatatc tgtaatcgtc
     4261 tctagtaata aatttttggc atgaactaag tcgtctcgct gtgtgttaag gaaggtcaat
     4321 cgttcatgaa cttcttcaaa ttgagcaatt gcatcaatat taacaggtcc taaagcttta
     4381 attttagctt gtagagattt caattgttca cgcgccatca aaatatcttc caagacattt
     4441 gcttttacct tcgcttcatc caatgtcatc tgatagtctt ctgataattg acgtgagaat
     4501 atcatcaatt gttgagataa ttgttcacat tgtgactcta attgagcttg ctgacgaata
     4561 aggctttcat ttttttgtcc ttctttagcc agagaagcag ctaaatcatc taaagcagct
     4621 tcacaatcct ccaattcaaa acggagactc acaagctttt cttcgtcatt ttctcttctc
     4681 ttatttgctt gtagcaactg tttttctata cgtggaagct cttctttgtc taattgactg
     4741 tcctgatggc ttagtaacgt ttgtaaatta ctaatgtcac gttttatttc agacaaggta
     4801 atctctagac gtgtacagtt ggcacgttca aatttctgct cgtttagcaa atcacgttct
     4861 tctagctgtg cctctgacaa taatgttgtt agattattga ctttttcttg aattgaatct
     4921 ttatcttcct taatttgagc aattgaagtt gtcaactctt cttttttaat agcaaattgt
     4981 tcaagttctt tttctaaacg atttttttca ttttcagaag tagtttgttc caacgccccg
     5041 ctgcttaatt gcaagccatt atatagttta tttaaatcag caagcttttc tgatagttgt
     5101 tgatactcta tatcagctct ttgttcctca agacgagctt gttctccatc attcttaagt
     5161 tgtgctaagg tttcttgttt ctctttgagt tgctctaaca atgcggcaac ttctttttct
     5221 tgaattaact gtttactttg tgcctgtttc aattcttttt ttagattatc caattcaggc
     5281 ttgataaaaa cagtattatt ttgacgattt gctccccctg agtacgaccc tccagggcgt
     5341 aactctgttc catctaaggt tactaaacgt acttgataat tcaattgtct tgcagctaca
     5401 ttagcattat caaccgtatc aaaaatagca gttaacccta agttattttt aaaaatgttt
     5461 gacaagcgtt gatcataggt taccaattca ctagctattc ctaaaaaccc ttgagaactt
     5521 tgtaattttg agagataatg ttgtgctaat tctcttggtt ttattgtagt taaaggcaga
     5581 aatgtcgctc taccttggcg gtttttcttt aaaaaagcaa ttgaacgttt ggcagcactc
     5641 tcgtcttcaa caatgatgtg ttggctgcta cctcccaaag ctatctccaa tgctgtttgg
     5701 taatgtttgt caaagctaag atgctcacta acagcaccta tgatacctcc aagctggtcc
     5761 ttagcctgta gaactgactt cactccagca tagaaattac tatgattttt caaaatagat
     5821 tctaaactcg aaatacgtgc ttgctttgat ttgatttcat ctaaatgatc aaataaaatt
     5881 gactgctgat tcttatactc accttctaga ttttgcaagg tttgacctaa atcttggtag
     5941 tgagataata actgacgaac attcttcttt gcagcctcaa atcgttccaa tgctaccttt
     6001 gcgttacttt ttaatacttc taaatttttg ctaacttctt gtatttcttg agatttagct
     6061 tgagaagctt gtttttgatt ctctatatca gctagtaaag ctgttaaagc gtttgaagtg
     6121 tcggcttctt cttgcatcaa ggtaacaaag tcttctcgaa gtttttcaat aacctgatcc
     6181 ggattagttg aaaaacgctc taaggaactt tctatactaa caatttctga tcttatttgg
     6241 gctatttttt ccgacagagt agttaactct atattttttt gggtaatctg gtcattacaa
     6301 tctttagctt taacttctaa ctctgctaag cgttgacctg cctcttcttt cttttcagcc
     6361 ttttggcttg actctaaacg aatcagatca atttggcgtt ccaaatctga ctttaacttt
     6421 gtaacgtcta aaagagctaa ctgtttaacc tcaatttctt cagataaatg gtgacgtttc
     6481 tgttttaacg attgattttc atcttcaagg gactgacgtt gttggtaata agttgctaac
     6541 tcttctctta ctgtcaatag cttttcctca acagtagtta aatctgattg atgttgtaaa
     6601 atatcttcaa tcaaaataga taagtggagt ccttgtcgtt cctcatctaa tactaaaaaa
     6661 cgcttagcaa tacttgcttg tttttctaat ggttggactt gcatgtccaa ttcataaata
     6721 atatcctcta agcgatctaa gttcccttga gtttgttcta atttggattg tgtttctttc
     6781 ttacgtgttt tgtatttcag aacaccagcg gcttcttcaa atatggcacg gcgctcttct
     6841 ggtttactat taaaaatagc ttcaacgcgt ccttgggaaa taatagaaaa agaatctctc
     6901 cctaatccag tatccataaa taaatcatga atatcacgta agcgaacttt gcgtccatct
     6961 attaaatatt cactatctcc gtttcgaaaa atacgacgtt caactcgaac ttcgtcagct
     7021 atattttcga taaagtggtc gctgttatct aaagtaacac tcacttgcgc ataattaagg
     7081 ggcttacgat tttctgtacc agcaaatatg acgtctggca tcttacctcc tcgtaaactt
     7141 tttgcgcttg attctcccaa tgcccaacgt aaactctctg tgatattact tttacctgat
     7201 ccatttggac ctacaacagc agtaactcct tgatcaaatt ctaccttagt tttatccgca
     7261 aaggacttaa agccttgcat ttcaatttct tttaaaaaca tgatttcctc attgtaacgt
     7321 tgctaaagca tttttggcag cgtcttgttc agcagctttc tttgatcgtc caattccttg
     7381 gctcaaattt tcttggttga cagatacagt cacctcaaat tcctttgcat gtgctggacc
     7441 actttcatta gtaacttgat aatctatttt tacatcccca tgagattgaa gtaattcttg
     7501 caagcttgtc ttgtaatctt taacacgctc ataggtccct ttttcaacgt gagggatcat
     7561 taccttgtta acaaaggcat gaaccacttc aacaccttta tccaataata aagctcctaa
     7621 aaaagcctca aataaatctc ctaaaatagt gtcacgatta cgtccaccag atttttcttc
     7681 tccttttcca agtttaatat agtgatcaaa gccacataga cgtgaaaaac ctgcaagact
     7741 ttcctcacga acaatcattg aacgtaattt tgataaatca ccttctgcct tttgagggta
     7801 ctttgtaaaa agatactgtg aaataagtaa ttgtagaaca gcgtctccta aaaattccaa
     7861 acgttcatta tgtgaaatgt ttaggaggcg atgctcattt gcgtatgatg tatgggtaaa
     7921 agctgtatct aataattctt gattagcaaa aacaatacca tagtcttttt ccagctttga
     7981 tcttaattct ttcatttttt ctccttgatt tcaatcatag ataaaatatt aaacaaatac
     8041 cattatacca cagttgacaa ataatcaaac attttggaag ttaaacttat cttccaaaga
     8101 aaacgtttcc ttaattacat atttgaatat tcaacaaaag actaggatac acacctagtc
     8161 tcttgttaac tattcatcaa ttttatcagg tactacataa aaaagcacat aagttcctac
     8221 aatagttcca atcaataatc ccagtttgag ccacattaga ggagcaaaat atattgaaat
     8281 ccccatcaag atataaattt gccaaatcat tgacttttta cgcgctgggg caatacttct
     8341 gctttcaaca aaatctgcag cgtagtattt ataaacttta gtttgacgca accaaatatc
     8401 aaatttttca gagcttctcg aaaagcaaaa agctgaaagc aatactaatg gagtcgttgg
     8461 aactactggc aagacaatac ctgcaatgcc tatccctaaa ctaataaatc ctaaactaat
     8521 gtaaaaaatc tttttcaatc ttaaatcctc gtcagtgggg tagctgaatc aggtgaagta
     8581 tcgtgaacag aaaaatctgt gcccacacca aagtcagctc tcattaaatt gttttccata
     8641 gtcatgtgag caagttcctt aatattattt tccttactca agtgtcctaa atagatgtgt
     8701 ttagtacggt tacctatagt gcgaatcatg gtttctgaac catcttcatt agataaatgt
     8761 cctttatctg ataaaatacg ttgcttaagt gtccaaggat aagaacccga acgtagaatc
     8821 tcaatgtcgt gatttgactc gatcaaataa ccatctgcat tctcaatcaa gcttgccatt
     8881 cggtcgctaa catatcctgt atctgtcaac atcacaaagc tcttatcatc tttcatcata
     8941 cggtaaaatt ggggatccac agcatcatga cttacaccaa agctctcaat atctaaatcc
     9001 ccgaaagtta aggttttacc gcgtccaaaa acatgctttt gactaacatc aactttgcct
     9061 aacatgttac gctcatccat aactttccac gtctgctcat tggcataaat atcaaggtga
     9121 tatttcctag ccaatacgcc aacaccttta atgtggtcag aatgttcatg ggtaactagt
     9181 atagcatcta aatcttctgg tttacgatta atttctgcaa gaaggctagt cactttcttg
     9241 cctgttaaac cagcgtcaat taaaagacgt ttttggggtg tctcaatata aaaacaattt
     9301 ccagttgaac ctgaagctaa gatactatat ttaaatccta tttctgacat gttttaatct
     9361 tcaacgtcct cccattcttc ataagttaca gcatctttat cataaggcaa gacgattgta
     9421 aaagtagacc ctttaccata ctcactctta gcccaaataa atcccttatg ctgcttaaca
     9481 atttcttttg caattgacaa accaagtcca gtcccaccct gttgacgact tctcgcctta
     9541 tcaacacgat aaaaacgatc aaaaatgaga ggtaaatctt ttttgggaat acctaagcct
     9601 tggtctgata ttgataaaat catctgcgtt ttagttgtgc gtagattaac tgtaatctta
     9661 ccaccatctg gtgaatactt gactgcatta tttaaaatgt tatcaatcac ttgagtcatc
     9721 ttatctgtat caatttccac ccaaattgac ttaagaggat aatctctgac aatttcataa
     9781 acttttcctg tgactgtttt ttgatttcta atctgatcaa atcgattcaa aattgaggtc
     9841 atgaaagctg taaaattcgt catttcaaca tctaaatgcg ttacttcatt atcaatgcgc
     9901 gataaactta ataaatctga tatcatacgc atcatacgat ttgtttcatc tagagatacc
     9961 ttaatgaagc ttggagcaac ttcttcattg agcgctccct catctaaagc ttctaaataa
    10021 gattttacag atgtcaaagg tgtacgaagt tcatgagaaa cattagatac aaaaagtctt
    10081 ctttcacgct cttctttctc ttgctcagta gcatcgtgtg atactgcaac taaaccggaa
    10141 ataaaaccac tttcacgacg gttcaaagca aatcgaatcc gtaaggtaac aaattcacca
    10201 ttttcatcac gtctattaag aactacctct ggcgtcttag agactaaatc ttggaacgaa
    10261 tatggacttc ccgaccctaa catatcaaca atattcattg ataaagcttc atcatatgct
    10321 agattgaact gctgttgggc tgtctcatta atcataacga tttttcctga acgatctgta
    10381 gccagcacac cgtcagtcat ataagttaag atactcgcta agcgattctt ttcttgttct
    10441 aagttatcat gtgccattcg gaagacatca gataaatcat ttagactttc ccctaattct
    10501 accaaatctg catcaccttg aattagaata ctatctgaat aatttcctgc tatcaaatct
    10561 ctgactttcc aattcaaaag ccggatatct ttagacattt taaaatctct aaccgctaga
    10621 taaacaaaat agacagcaac aaaaactagt aaaaaaagta aggctagctc aaacgaccta
    10681 atatttgcag cactattatt cataagactt catgtaataa cctacaccac gtcgagtcaa
    10741 aatatattct ggacgaccag gagtatcttc aatcttttca cgtaaacgtc tcaccgtcac
    10801 atcaactgta cggacatctc caaaataatc atatccccaa accgtttcca ataaatgttc
    10861 gcgtgtcatg acttgtccta tatgtgtcgc taaatggtga agtaattcaa attctcggtg
    10921 cgttaattca atttcttcgc cacgtttctt agcaataaag gcgtctggta aaatttgcaa
    10981 ctcaccaata gtaatatctg aagaagcatt ttgagcactt tcttctgcta cagctgtttc
    11041 aatattttca gtacgtcgta aatgtgcttt gacccgtgcc aaaagttcac gatttgaaaa
    11101 aggtttagta acataatcat ctgcaccgat ttctaaacca atgactttgt caaactcact
    11161 gtcttttgcg gaaagcataa taataggaat atggctggtt ttacgaactt ccttagccac
    11221 ttctaaacca tctagttcag ggagcattaa atccaaaatg attaaatctg gttgaaattc
    11281 tgcatattgt actaaagctt cacggccatc gaaagctgtc gccgtttcat atccttcttt
    11341 ggttaaatta aatttgataa tatctgaaat tggtttttca tcatcaacga taagtatttt
    11401 tttcatattt tacctctatt actttctggt ctcccacttc catccataaa ctgaaaaata
    11461 aaagtgaccg aaaatatgat aatatgggcc taaagcacca ctattatcac taattataac
    11521 agatttccta gttaattgga aactttcaat acatcctatt aaactgaaaa ataaatgata
    11581 cgagaacgta atatttttac tagaaatacc gctctaaaaa tagcaatgtt taaatcaatc
    11641 ctactcttta atattctgaa tttattgaca agctcacaaa caggtgatac acttatctat
    11701 aatgttaggt aacctaacaa taaactgtta ttttaggagg ttcttatgtc actcatatct
    11761 tataagaatg ttaacaagta ttatggagat tatcacgccc ttcgtcagat taatttggaa
    11821 atagagccag ggcaagtggt tgttctatta ggaccttctg gatcaggtaa atcaacactc
    11881 attagaacaa tgaacgctct cgaatctatt gacgatggta gtttggtcgt gaatggacat
    11941 gaattagcaa atatttcatc taaagaattg gtcaacctcc gtaaagaagt tggtatggtt
    12001 tttcaacatt ttaaccttta tccccacaaa accgtgttag aaaatattac attagctcct
    12061 attaaagtat taaaacaatc taaaaaagaa gctatggaaa ttgctgaaaa atatttaaaa
    12121 tttgtcaata tgtgggaaag aaaagattcc tacccatcta tgctatcagg tggtcaaaaa
    12181 caaagaattg ctattgctag agggcttgcc atgcatccaa aactcctcct ctttgatgag
    12241 cctacctctg cccttgatcc cgaaaccatc ggagacgttc tttcagtgat gcaaaaattg
    12301 gcaaatgacg gaatgaatat ggtcgttgta actcatgaaa tgggattcgc acgagaagtt
    12361 gctgatcgta ttatttttat ggctgacggg gaaattttag tagatactac ggatgtgcaa
    12421 gacttttttg ataatcccac agaaccacgt gccaaacaat ttttaagaaa cattatcaac
    12481 cataccagta ctgaccttta ggaggacgaa atatgctgag acgaaaaaga ttaacgctct
    12541 atctattatc ctgtattttc attttcctgt tattttatcc caactcaacg tcagctaatc
    12601 aattatcaga aatcaaaaaa tcgggtgtcc ttaaagttgg agtcaagcaa gatgtgccta
    12661 actttggtta ttataatgct gagacgaatc aatacgaagg aatggaaatt gatattgcca
    12721 aaaaaattgc caaatcctta ggcgtcaaac cagtttttgt accgacaact gcacaaacca
    12781 gagagcctct gatggacaat gggcaaattg atattctaat cgcaacatac actatcacgc
    12841 ccgaaagaaa agctaactat aatatttcta aagcttatta tcacgatgaa attggatttt
    12901 tggtgcgtaa aaatagtcat atcaaaacta taaaagaatt ggatggtaaa cacattggtg
    12961 tggctcaagg tgccactaca aaagtcaatt tagaaaaata tgctaaagaa cacaagctaa
    13021 aatttagcta tgctcaatta gggagtttcc cagaattagc gatatcgctg tacgctaatc
    13081 gtattgatgc tttttcagtt gataaatcta tccttagcgg ctaccttagc cctcatacaa
    13141 ccatattaaa agaagggttc aatacgcaag aatacggtat tgcaaccagt aaacaggaca
    13201 aagtcttgat tccctacgtt aataagttac tggtatcatg ggaaaaagat ggtagtttaa
    13261 aacacattta tcaaaaattt aagcttaaac ccgctaaagt aaaaaaagaa tagggaggat
    13321 tttttatgac tttattaaac atcagtcctt ttgccatttc aaggtggggc gctttcttta
    13381 accattttga cctattcttt aaaggcttct tatatactct aggtatttcg tttggtgcac
    13441 tcctacttgc tctgatatta ggaattctct ctggtggttt atcaacttct aaaagtaaag
    13501 ttggtaagtt gatatcacgt atctatgttg aagtttttca aaacacacct cttttggttc
    13561 aaatggtttt tgtctactat ggactagcaa tcattagtaa tggtcatatt atgattagtg
    13621 cttttttcac cgctgttttg tgcgttggcc tataccatgg tgcctatatt tctgaagtta
    13681 ttcgctcagg aattgaagct gtccctaaag gacaaacaga ggctgcttta gctcaaggat
    13741 tcacagctaa tcaaaccatg caacttatca ttttgcctca agctatcaga acaattttac
    13801 caccaatgac caaccaagtt gttaacctaa tcaagaatac ctctaccgta gcaatcattt
    13861 ctggagccga tatcatgttt ttagctaagg cctgggctta tgatacgact aattatatcc
    13921 cagcttttgc aggagctgct atcttctatt ttgttatttg tttcccacta gcaagctggg
    13981 caagaaaaca agaagaatta aataagaaaa cttattctta aggaggtttg atatgtcatc
    14041 agtcttaaca ctaacaaata tactatttct ccttcaaggg ttcggtttaa ccttatatat
    14101 atcttttatt tccattttac tatcaatgtt ttttggaact ctattagcaa ttatgagaaa
    14161 tagcaaaaat cctatctgga aattaatagc tagtatctat atcgaatttg tcagaaatgt
    14221 tccaaatcta ctttggattt ttatcatttt cttagtattc cagatgaaat cagtcagcgc
    14281 tggtattact tcgtttacaa tttttacctc cgctgcttta gcagaaatta ttcggggagg
    14341 tttaaatgga gttgataaag gacagacaga agctggactt tctcaaggat tcacttattt
    14401 gcaagttttc attattatta tttttccaca agcttttcgc aaaatgttgc cagccattat
    14461 ttctcagttt gtaacggtta taaaagatac atcactactt tattctgtta tagctattca
    14521 agaaatcttc ggaaaaagtc aaattctaat gggaagatat ttcgaagctg gacaagtttt
    14581 tacactctac gctattataa ctgctgtcta tttcattaca aactttatca tctcaagttt
    14641 ttcacgtaaa ctttcaaaac gttgggagca agctactgag taattaaaac gaaggccttt
    14701 tagaccttcg tttttttata tcttatttaa attaaattat atatgccttt ttgactatct
    14761 ttttatatct tctcaaatgt ccgcaaagcc ttattttatc ggctctacgg gcattttgct
    14821 tttgtggtaa acctcacata tctaggtcta tctttttata ttttcgctat caaacgtggt
    14881 taaaatcgtg gtaaatactt ctatgcgatt agacgctgaa cctctgattt tgcggtatct
    14941 atggacgcat gagcatacca gttcattgtg atactgatgt ttgaatgtcc catgatatac
    15001 tgtaaatctt tcgggttcat gttcttgctt gccagccttg tgcagaacgt atggcgtagc
    15061 gtatgcggtg tgatatgtgg caagggattg tccttgtgtt gcttgttgta tttctttacc
    15121 atacggacaa ataaggcatt gtaatcaatc gcaactttgg gcttgccttt atgattgaca
    15181 aacagaaaat tgctccgtcc gtctatcaca aatggttctg cctttgggcg ttttttcata
    15241 acccgttgaa atgcctgtat tgtttctctg cttaatggca cttgccttgt tccgttcttt
    15301 gttttaggcg tttcaatata atagccctgt tccttgctct ttaataactg gtggtcgata
    15361 atcacaactt cattcttgaa atcaatatcg gctactgtca gtccgcacag ttccgagata
    15421 cgaagtccag tctttaacag tatcagcaca tcatcataat acttatgata cacgttgtcc
    15481 gtcttaataa atgacagtag ggcttgttct tgttcctctg tcaatgccac tttctctttg
    15541 gtatcatttt ctaggacttc acttaacttg aaatcaaaag ggttcttcct tacacaatcg
    15601 tcttgtatgg cgatatagaa tgacgctttt aacgagcgtt tatggttgtt aatagtattg
    15661 taggaaaagc ctttctcttt catgcgtaac gcccattcct tagcgtcaga gggttttatc
    15721 gtatcaatgc tcctagcacc caacttgtcc tctttcaata accgcatgag ctgttcccgt
    15781 tgtttcattg tgcttttctt cacgtttgcc ctctgttcgt tctgtttggc gtagagttgg
    15841 caaagcgtca ttttcttgcc tgtgctgtcg ataccgtcct caatatcccg tcttatctgc
    15901 tgttccagtt cacgaagtga ggttttttcc cgttttccct ttggtgtcgg gtctgtgggt
    15961 gtcaatctcc aagcatacac atattttgta tttccaaatg cgtctacata tttatacaag
    16021 tattttccgt ctgttcgttg gctctctcca gtatgcagga tacgtccttt gttatcccgt
    16081 cttttttctt tcatggtgtc tgctcctttc cttgttggaa agagccttga tatgacttgt
    16141 gttcatcata acacatacaa ggctcatttg cattagattg cgtccaattt atcaataacc
    16201 tgttcaaact gtcgtcgttt aatctgtatg cggttgccat tcataatgag ccagccagcg
    16261 tccttgtttt cctctgccaa gcgtcttaac ttgttttctc cgatacggaa atactttgac
    16321 gcttcttcaa tggtaagggt gtatttttcc catacgggaa tatcctgtgc attactcata
    16381 aaacaccccc tcactttcaa ttacgtctgc tatgtccgta cacatttgtt ccacaagcat
    16441 ttcctgtttt tcagaaaggt gggtacattc atcaatcatt ttcatgtagt ttgagcctgt
    16501 cagcctgtcg atttcctcta tcatcacgat tgacggcatg acctgtcttt ccagccattg
    16561 cagagtgcgt tccatattga caggtacggg gttcatttca aaaggtaaac tatcatggga
    16621 aatgaatatc tcccacagcg gatagtcggg aaattgcaca aaatcagtaa taaggtaaaa
    16681 caccagccca tgagggttat acgtcaataa cagttcctca actgcctgta ctgccttttt
    16741 gtcacgcaga cgaatttcaa aacggttgat aatatccgta tgtttctttt tagttgcctg
    16801 ttccttttgt ttttcataca ggcagaaata ttttgtgctt gcttttgagc cgatataaag
    16861 ggtgcttgct aaattgcggt tttttccgct tagtttaccg ccttgcacat tttcatagtt
    16921 tttacagcga caatccgcac cgccattttt gtatttttca gataaaacgg gaatatccag
    16981 cagaccgcac atatcgttga ttgccaagtc aaaacgtcgg ataacaccgc cacagtcaag
    17041 acagcggttc aagaatgaat accaatccct gttctgtgcc tgtaaaacat attccatatt
    17101 gcgtgagcct gcccctttta attccaaaaa tacgcccatg tgttcatcat cagaagccat
    17161 aaccttgatt tctccgtagg cgtaatgctc tttatagcca taatatccat agtcataatg
    17221 gataaaataa tctgctttca tgccaagcac ctttcggata atttccaatg cgtcagtcgt
    17281 tggaaaacgg acagacagat aatcaacgag cagataaaaa ggcttatcgc agatatagta
    17341 attcagacaa cgcaggataa tattttgtgt ttccttgctg gcgttggctt ttccgttctc
    17401 aaagcggttg atactatatc ttgatacatg ggtaagactt gcaagttctc cttgtgaaag
    17461 accgcttgca agacgtttat tcttcatttc aagtgcaaat tctaaattgt tcattgtatt
    17521 cctccaatcc cacaatagcc ttgaaatacc tgtaaaccac tattcaaaag aatgtgaaat
    17581 tactatcttc ttaaaactcc tcattactcc ttacagcttt ggggttgtat ggtttttgac
    17641 ttgtcttttg tgaaatagtg cccccctgtt agataacggg ggcacagaca aggctcgcag
    17701 gctcgccagc cgacaaagca tttcttcccc ttgacacaag gtgtcatggc ggtaagcctg
    17761 agaggcttcc gccaccgtgt cagaaatact ttgtcagcaa gttctgtttt gccttttcta
    17821 acaagttcca tttcaaccga tattcatatc tacggattta atcagatagg tatttctttt
    17881 tgaatgtttt tatatttcat tctattgtgc ttattcgatt acaagaacat agtaattctt
    17941 atatgcttat ttgtcaatat ctttttttgc ctttacaaat catattaagc atgatagact
    18001 tataatagcg aagatataac gagatatgga gggttatgca atgaaaataa gccatttagg
    18061 aaataatata cagaccataa gaaaatttag gggaatgaaa caacaggaac ttgcggacaa
    18121 aatcggtatc aatatgcaga gcctttccaa gattgaaaga gggttgaact atcctgccta
    18181 tgaaacgctg gaaaagataa tggaagtgct ggacgttacg cccaatgaat tgttgtcggg
    18241 agaatggaag tatgtcaatc aatctgaaaa ggaagtctgt cagtttttaa gagccgaaga
    18301 acgtctaaat gcagaactga aacatggaca ctatgataac ttctttgaca gcgaggaaga
    18361 atggctggaa tatgagttag aaaagttacg ggaatatatt acagactata tcaacgggaa
    18421 aagcattgaa gcgtccgacc tttacccaat caaagaattt atccagcatt tgaagtttca
    18481 aaaattatta gaccgctatg atgatttata cagtatggat atgtttgggg aaagcataga
    18541 gggacataag tataagacac cttatcaagt cgtgaaaatg ataaacccga actcaaaaga
    18601 agatatggaa ctgttacgag aagtattaag gaataacaat tttgatgatg aggacgagta
    18661 acttttttcc tcttgcgaga agatacagca aacaaaaagc ccgatcaaga gtgcaaagca
    18721 ccaccaatgg tggcaaagct cttgacaggg ctttttcttt tctgttgtgg gcaatcaaga
    18781 ggaagaaagg gataaaatct gatttgtggt aaatcaggca aaggcaacgc aggtatctca
    18841 atttgatttg gtacttgaaa agaacgggag taattggaag attatagaat aacaaatatt
    18901 ggtacattat tacagctatt ttgtaatcac gtactctctt tgataaaaaa ttggagattc
    18961 ctttacaaat atgctcttac gtgctattat ttaagtatct atttaaaagg agttaataaa
    19021 tatgcggcaa ggtattctta aataaactgt caatttgata gtgggaacaa ataattggat
    19081 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
    19141 ttctaaagta tccggagaat atctgtatgc tttgtatgcc tatggttatg cataaaaatc
    19201 ccagtgataa gagtatttat cactgggatt tttatgccct tttgggcttt tgaatggagg
    19261 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgcg ggaaaaacta
    19321 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
    19381 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
    19441 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
    19501 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
    19561 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
    19621 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
    19681 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
    19741 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
    19801 taatagaggg aaacgatgac cttttagaga aatatacgtc tgggaaatta ttggaagcat
    19861 tagaactcga acaagaggaa agcataagat ttcataattg ttccctgttc cctgtttatc
    19921 acggaagtgc aaaaaacaat atagggattg ataaccttat agaagtgatt acgaataaat
    19981 tttattcatc aacacatcga ggtcagtctg aactttgcgg aaaagttttc aaaattgagt
    20041 attcggaaaa aagacagcgt cttgcatata tacgtcttta tagtggcgta ctgcatttgc
    20101 gagattcggt tagaatatcg gaaaaggaaa aaataaaaat tacagaaatg tatacttcaa
    20161 taaatggtga attatgtaaa atcgataagg cttattccgg ggaaattgtt attttgcaaa
    20221 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
    20281 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
    20341 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
    20401 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
    20461 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaaataaaa gagcctacag
    20521 tcatttatat ggaaagaccg ttaaaaaaag cagagtatac cattcacatc gaagttccac
    20581 cgaatccttt ctgggcttcc attggtctat ctgtagcaca gcttccatta gggagcggag
    20641 tacagtatga gagctcggtt tctcttggat acttaaatca atcgtttcaa aatgcagtta
    20701 tggaggggat acgctatggc tgtgaacaag gattgtatgg ttggaatgtg acggactgta
    20761 aaatctgttt taagtatggc ttatactata gccctgttag taccccagca gattttcgga
    20821 tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa ttgttagagc
    20881 catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca tacaacgatg
    20941 ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag gtcattctta
    21001 gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact ttctttacaa
    21061 atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc ggtgaacctg
    21121 tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg ttcaataaaa
    21181 taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata agatgaaata
    21241 ttttttaata aagatttgaa ttaaagtgta aaggaggaga tagttattat aaactacaag
    21301 tggatattgt gtcctgtatg tggaaataaa acacgattaa agataaggga agatactgaa
    21361 ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag
    21421 cagttcaaag taactgtgat tacagagcca gacgcaaaga cgcagagccg ataaaatgag
    21481 attaatacaa tctcatttta tcggctcttt ccgttatgta tggattcttt taattagtct
    21541 tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt ccattaagga tagttctttg
    21601 tctgtaaagc tatccatgta tttctctatc tgtaatcgtc gggtgctttt taccaagtta
    21661 ttagcaggta agaaaaattc atcaacggaa acatgaagta acgatacaag gtcataaaga
    21721 acttgtatgc tggggtgttg ccctttattt tcaatattag ttaagtaccg tgggtcaatt
    21781 tcaatcaatg ctcccacttg ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg
    21841 gctaaaccaa aggctctaaa atcatattta tcttcttttt tacgcatagt agaccacctc
    21901 tatacatttt attgttccta ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt
    21961 tataggttta tatttaataa aaagcactac taaacgccaa taaaaaaaac cgttatatgg
    22021 tagtgctatt tacgctgtta aaatattgta tattacttcc aaatggcggt ttgttggagg
    22081 tcaacgtcgc catgaagtac atcatataca ataaatttcc ttacattggg ttcttgtcaa
    22141 aaaaagtcgt ctatctgcaa tagataagta cgtccaccaa tgtggtttta taaatcatat
    22201 agatagaata acagaagcat gtaaacagag aaataaatct gtttatatgc ttttttggct
    22261 attcagaact tttttacaaa gtttatttat cagtaatgca acaaatcccc ctttcacatt
    22321 gggactaaga gtgaaaggag ataaacgagc aaggctcact tcctttccta gacagaaagg
    22381 gggtgagaaa catgaaacca tcttcttttc agaccacaat agaaaatcag tttgactata
    22441 tctgtaaacg tgctatggaa gacgagcgaa agaattatat gctttatctt tcaaggattg
    22501 caaagcgtga ggtgtccttt tcggatgttg gcgattatct tgttagccag tttgcgacaa
    22561 cagataacta ttcaactgac tttcagattt ttacactcaa tgggttatca gtaggcgttg
    22621 aaaatgattt gttgagtgaa gcattacgtg agttgccaga caagaaacgt gaaattctac
    22681 tgctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag tttcctactg
    22741 ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg ttgttcaaat
    22801 ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa tggctacgct
    22861 ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta ttttattcga
    22921 tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt caatcatgat
    22981 ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg ggttaaatag
    23041 tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa ggatagtgtt
    23101 ataaaagaaa gagtaatatt gcagtattta aggctcgcac aaatatatgt tttgtgtggg
    23161 cttttttctt accatttttc tgtatttcta aagggtataa acggtattcc gtttatatat
    23221 ttcagcatta gcagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    23281 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    23341 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    23401 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    23461 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctgtcagaaa
    23521 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga aaaatttcag
    23581 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    23641 taaaaatgct tttgagcgaa gggaagtaaa gaaatgagta aagattatgg ctatccctct
    23701 tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga gattttgaag
    23761 ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc tgaaagtggt
    23821 aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga catgatgaaa
    23881 gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa tcgggagata
    23941 cacagtttcg tgctatctcc cttagtagtt ttacggtcat atcaaggctc attcaatcgt
    24001 ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa tgtatagtgt
    24061 ttatgcggat aattgaaaat tagatataaa atttctatta atcattcata tgatctctag
    24121 aacgttcaat gatttttcca gtttctgcat caatttgata atcaacttca tttttatttt
    24181 tcaggtccac aaagtcaata tcataaactg attttccatt ttcaatttct tctttgatct
    24241 taagattact gacttctgat tcctctatat tagcatcttt aaatgcaatc tttttggctt
    24301 cttcttgact tattttatga gatttttgac ttgacgatga acttgactga gaagtcgatt
    24361 ttgaaaagcc attattatcc atgtcccgct caaggatttg tccagtctta gcatctacat
    24421 gatagctgta ttctgcgctt ttggtattga attcaatttc gtagctccct ctaaagtttt
    24481 caaattcttt gtcaattttt tgatgtatga catccttctt agccacttta gcatctttat
    24541 aagcaatagc taacgcttga ttagttgtct tttggaattg tgattggtaa aataatacac
    24601 cacctccaag gataacaaca aggactaaaa gaactcctaa aaaaacttta tttttcttaa
    24661 tcatacagca ccttctttct attattcaat tgtattttaa cataagctag acactttttc
    24721 aattttgacc actcgtttat aaaaatatgt ggtcaataaa aaccatttga cattaatacc
    24781 gcaaattaaa agataccata gtatcatggt atcttttaat agtatatgta tcagttgatg
    24841 cgcattttct gcacagcctt cttcaagtat ttgacttgtt tatcaaccga ttgcttagca
    24901 ctacgccttc caaatgcgta atatcctttt ttattgaaat aactacaatc gtaatcctct
    24961 tctttaaaga aaaaccattt ccttctagat agatttggcg atctataacc tctagtcata
    25021 tcgtttcgaa attttgggtc taactttaca ggatcaacat ctaattcttt atgacgtgta
    25081 ttatttttaa aagcataatt aaagctagca ccgttgataa tgccattacg tgtgattact
    25141 tctgcgtcaa tctcgtttaa aaagtttcct ttgtcatcaa taataaattc cgtatggaat
    25201 gaaattaaaa gcttactatt aataaccggc tcatcatctg ggaaaccaaa gatttcacca
    25261 tttctccgac gtttattatg caagcgagca gattcatagt aggcgatact atctggttct
    25321 cttaaacatg ataggtagtt tactaaagca tctctatcag tcataccaac atctttaaag
    25381 tatcgtcgaa catactgtgc ttgctgacta gaaatcaaat agcggaattg atggagtttc
    25441 ctatttaata cagtatcttc ggctagacta ttcccaggaa aagaatcata aataacttgt
    25501 gataaggcta tccagaaatc atcatgcgga gctaactcat ctgaaaactg tgcgaccaag
    25561 tattgtgttt ctgataattc tcctgacaat tcagttggca ttttagcctg ctctaacacg
    25621 aattctaaag ctttgagggg tgcttgtaaa cttaaagaag tccaaaaata cttaaaaata
    25681 ggagagccaa cttttatatc atcaatagcc ttgagtccct ttaaaaagag taatagatca
    25741 tctttaggta tgctccattt atctgctaaa gacagtatca tttttatatt ttgataataa
    25801 ttttctatcc cttcaactgt aaaaggaata ttaagagcgg tctttgccat atctctcccc
    25861 tccaatttta cttattatag caaaaaggaa agcttaaaac caagaaatat cttcactaaa
    25921 aaatggctct cactagccat tatgttttta aacaagatac tgcggttttt cgattttata
    25981 ccctactcct ctaagtgttt ttatatattt aggatattta ctatcatctt cgattttgtc
    26041 acggatatta gaaatatgca ccataatcgt tgtctcatca ccttctaaat acctaccatt
    26101 aatcttctca tagagttgtg ttttagtata aatctttcct ggtgatgaca ttaagatatg
    26161 aagaatctta aattctgtag cagtcaactt gatatgttta ccatttttag ttagttcaac
    26221 atgttcctca tctagttcca attcccctat ctttataaat tgatttttag gtttggcaag
    26281 actattgaat tcatagtaac gacgcagttg cgaattaatt cgagctaaaa cctccaaaga
    26341 attaaacggt ttcgtgataa aatcatctgc tccaatattc aatcctaata ttctgtccat
    26401 atctgttgtc ttagctgcta gcataataat aggaatctga ctatcttgac gaatgagtcg
    26461 cgtcaactca tatccatcta ttttaggtag cgtaatatct atgattgcta aatcaatctg
    26521 atgttgctga aataaagaaa aagcctcctc accatcacta gccatcacaa ccttataaag
    26581 agctccctct aaatacgacc ttaataactc tattgtttcg tcatcacctt gcactactaa
    26641 tactgttcgc aatgctccat cccccttttt attaatacta ttttccttta tgtgtcaatc
    26701 atattaatta taccaaaaaa tctaattttg tgaatgttat atgagattat ttatctataa
    26761 ttagcttata attaaacatc aagaaaaaat tagcatctaa aaaactgatt ctgcatcata
    26821 cagtaatcag ttcacatcat ttattaatga tttttataat gactaataaa atttatttaa
    26881 cttcgtcagg tctagatttt cttgcaatat cagctaaaat actttctaca aattcctcta
    26941 tagattttgt ttcagtcgct ttacttccat agcgacgcac atttacggct ttttcttcca
    27001 tttccttatc accaacaatc aattgatatg gaattttttg ggtttgcgcc gcacgaatct
    27061 tatattgcat cttttcgtta cggtcatcca cttcagcacg aataccacga tctttaaggg
    27121 cacgagccac ttcccaagca taatcaatgt gggcttcatt tgaaattgga ataacagaaa
    27181 cttgttgtgg agcaagccaa gttggaaagg cacctttgta agtttcaatt aaaatagctg
    27241 taaatcgctc cattgttgaa ataccaccgc ggtgaatcat aattggacga tgttcttcac
    27301 catctgcacc gatgtattta agatcaaagc gttctggaag aaggaaatct agttggatag
    27361 tagataatgt ttcttcatta ccaagagcag ttttcacctg gatatccaat ttaggaccgt
    27421 aaaaggctgc ttcgccttct gcttcaaaat aatctaatcc aaaatcatcc atagcctctt
    27481 ttagcatagc ttgtgcattt tcccacatct catcgttatc ataatactta tgtttatctt
    27541 ctgggtcacg ataagataag cggaaacggt aatctgttaa gttaaaatct tcataaattt
    27601 ctgctatgag attaagagct ttaagaaatt catctttaat ttgctcaggt gtaacgaaaa
    27661 tatgggcatc gttgagtgtc atttcgcgta cacgttgcaa tcctgtgagg gcccctgatt
    27721 tttcataacg gtgcatcata ccaagttcag cgatacgaat tggcaattca cgatatgagt
    27781 gaacatgatg tttataaact tcaatatggt gtgggcagtt cattggacga agaacgaatt
    27841 cctccccatc tcccatatcc attgttggga acatatcttc acgatagtgg tcccagtgcc
    27901 cagaagtctt gtaaaattca acagatgcca tcggaggtgt gtaaacgtgt tgataacctg
    27961 aagcaatctc cttatcaaca atgtaacgtt ccaattcgcg gcgaatagtt gctccatttg
    28021 gcaaccagaa tggcaatcct tgacctactt caggattaac cataaataaa tcaagttctt
    28081 tacctaattt gcggtggtca cgttccttag cttcttcacg gcgtttaaga tatgctttca
    28141 aatctttttt atcaaaccaa gcagtaccat agacacgctg catcatagca ttatcactgt
    28201 taccacgcca gtaagcccca gcgacattta gaagatgaaa gacttgaata cgtcctgttg
    28261 atgggacatg aggtccacga caaaggtcta caaattcacc ttgacgataa acagttaaac
    28321 catcttcagc atgttcacta attaattcaa ctttataagg gtcatcttta aaaagttcta
    28381 acgcttcttc tttactgatt tcttcacgaa tacaagggtg attttcctta acaatttttt
    28441 tcatttcttc ttcaatacga ggaagatcat catttgaaat ttgacctgat ttattatcag
    28501 tatcgtagta aaaaccatcg tgaatagctg gacctacacc taaacataag tctggaaaaa
    28561 ggcgttttgc agcttgagca aataaatgcg ctgctgagtg acgaagaact cctaaagcat
    28621 cttcgtggtc aggtgttaca atctcaatac taccatcttc ttcgatagca cgtgttgtat
    28681 caataagttg accattaaat ttaccagcta aagctttttt agctaaagag ttagaaattg
    28741 attgtgcaat ttcaaatgtt gtgatgccag attcaaattc acgaattgca ccatctggga
    28801 aagtgatttt aatcatcttg ttctcctttt cttaataact gttagggtga taccaattcc
    28861 taaacacatt aattcactaa taaacaaagg gattgacctt tgattgaatt ccacattatc
    28921 ctagataggg ataataagta taacattaga aaagcaatct gcctctttta tgcaaccact
    28981 tcttttataa aaaaagagcc tttacatcca aaaggacgta aagactacgt ggttccacct
    29041 caatttatcc aacacaaaaa ctcgagtata cttgtgtcag acctctagtg gcgataaggg
    29101 aaccaccctt ttatgtttac ataaaatcaa ggaagtagta ctaagttctc taattgcact
    29161 ctttccacca accagtgctc gctcttgaaa tctattagaa cctaacatgt cttcattttt
    29221 tattataaca atatttttta taatttcaag acatttacta attttattcg tctcaatcta
    29281 aaaaatctcg aatagatgaa atacgacgtt taggtgcttt taccactttt ttagaggctc
    29341 ttcctgtaaa ggtaaaaact ttcttaggta aatataccgc ttttctgaca aaacgaagag
    29401 tagaatggct atccttattt cctatcgtca gagagacttt ttctttcaca ctagcttttt
    29461 gtgaaatcag aaagtctagg taaaaagcat atacagactt gctgaaattc tcagcagaaa
    29521 tctcatataa tttctgttca taagcttctt gagtcatttt gggtgtctct gctattgcat
    29581 ctaggatagc atctgccaaa tcactttcct tcttgtacag agttccaaac attttatctg
    29641 aaataacatc atctagataa ggattgcttt gcgcaatgat tggacgtcca ctcgccagac
    29701 tctcaatgta agttaatcct tgcgtttcac ttgttgaagc actaataaag aaatcacatg
    29761 ctttatagta gatagccact tggctatgct caaccatacc tgtaaaaata actgaatttt
    29821 caagttctaa agagtgaaca agttctttta gatctgaaag ataaggacca tctccaacta
    29881 tcactaattt tacgtgagga ttttcatcaa cgacagctga taaatgcatc aagattgctt
    29941 gaatattttt ttcaaaagat acccgtgata aactcaatag cattgtatca ttatccgcaa
    30001 ggccaagctc cgttcgtaga ttcttgattg tttcttcaga gatatcctct cgacgatagt
    30061 tctcaacagg aatacctgtc ggaataactt gttttggaat tttgacaccg taaccatcta
    30121 gaagattgag aacaattcga gaaggacata tgacaccatc caaatcactt aggtaggtac
    30181 gcatgatata tttgaccata cttggcttaa tcaatttgcc ttttgcgata taaccaacgt
    30241 aatcctcata ttgagtatga taagtatgca caacaggtat tcttaaagct tttgcaacta
    30301 attttcctaa aagtcctaaa ctgaattctg tttgagtatg aataatatca agttcgtagt
    30361 ctttagctat cctataagca gaaattaaac ctctataaac tactcgacga tctgtaaaag
    30421 aaataaaagg gacacttggt aaacgaataa ttgtagggtc ttcaaaacgt ttgacattac
    30481 ggtcggtagt tgtgaaaata taaacttcgt gtccttcctt ctccaaccct tcctttaaag
    30541 ttcggatact tgttgaaact cccgaaactt ggggaaagta tgtatctgta aatagaccta
    30601 tacgcatctt acaactccat tactttttga taaacacttg ccaattcatg ggcaattcgt
    30661 tcgatacttc gactctcagc cacacgataa ccttctttaa tcttatcact cttaccagat
    30721 aagactttat ccaacttttc aacaaaacca tcaacatcag tcgctaattc aacactgtct
    30781 tcggtcaccc aaccgtgata cacagggata tctcttaaaa ccacatgctg atgactagct
    30841 aaagcctcta aaactactat cccttctgtc tcttcacgac tcggaaagaa gaaagcatcg
    30901 ctagcactca tggcaccttc ataaacatca ccttttatgt aaccagcaaa agtaacattt
    30961 gagggatgtt gcttggtaac gatttgccta acctttcgag gaataaccca tttatttgtt
    31021 tctccaaacc aaataaaacg aatatcaggc atttttgcag ctacttcaac aaattggtca
    31081 attcccttac gcatgaaata aagaccggcc cccataacaa ctttatcatc cttacttaag
    31141 tgaaaatagt gacgaaatgc agcttctttc ttttctgacc tctgatagcg acttagatca
    31201 atcccgttag ataaaacaaa aattggtttc ttgatgccat atgcttttat tagttgttta
    31261 gaataatcag ttggggttat aatggcatct gctttttgat aaaaacggca aaggtaccat
    31321 ttaaaaagcg gggatactaa atttgagcca ataaaagaat ttctaaagtc ctcttcagtg
    31381 gaatggccat gcataattac ttttttccca gtttttttag ctttactcat caaaagccaa
    31441 cttctaatac cataggtatt catatgaaca aggtcaaagt catctgtagg attagtggta
    31501 taatctatcc cagcaatttg aagagctttc tcttgatgtt tgatagcacg accgatacct
    31561 gactttttca agtattcttc agcctctaaa tacagtaaaa ctttcatagg tcttattata
    31621 tctcatttta cctttttttt cacctgattt atcagttagc ttcctctatt ttagggaccc
    31681 aagccgagat agacgcagat gcaactaaaa aatcacccca accatcatct cctataacaa
    31741 cctcttgttc acaattgcct aaataatcaa caaaagtttt gcctgcataa atttccccta
    31801 cttccatatg tttccatccc tgatcaccat ttgttaggac cacagctaaa caagaattat
    31861 gttcctcatc cccaagacaa gtccaaccaa tacaatttga atgcgtaaag taatcgactt
    31921 gttcgccaaa tacatagttt tggcgtagct cagccatttt atcaataacc tctttaaagg
    31981 atggttggcc aaattctcct tgaatgccat agtaatcacc ataaaaaaga catggagtcc
    32041 cttgttggcg taaaagtatc aagccataag ctaaaggttt aaaccaatct tccactctac
    32101 tttctagagc ttgtccactt tgcgtatcgt gattttctac gaatgtaaca gcatactcag
    32161 gattatcaat aacaagacta tcatcaaaaa ttgttcgcat atcaaaatca cggttttggt
    32221 gactggcatc aaaaaaattc atatgcaagg tgacatcaac aagagaaaat tgaaattgtg
    32281 ttgcctctaa atagtccttc attgatgttt ggtcagattt ccaatattcg ccaaagacct
    32341 ctaaatcggg ttttattttt gtacgaatat cattaatgaa agtttgaata aagtaagaat
    32401 cgatatgttt cacagcatcc aaacgaaaac cttcaatgcc tgtagtttca ataaaccact
    32461 tagcccaatc ttgtaagttt tttataactt caggatgctt aaaatcaatg tcattgtaca
    32521 tgagataatc gaaattacca ttttcatcat caatcaaatc atcgtctgcc caacctttat
    32581 tatcgcctac aatcataaag atgtctgttt cattattttt agcatcgtaa tctaaaccag
    32641 taaagtggta ccagtgccat ttaaaatcat tgtactcacc ctgtctacct gggaaatcaa
    32701 atcccgtcca tccttcaatc tcatagggtt cacttaatgc ttcttgacga ttttcaggat
    32761 tgactttgat gacttgaaat ttttctttat gatcaccatt ggctttatgg ttaagaacga
    32821 tatctgcaaa cggtttaatg ccatttgcct ttaacgactt aataagcttt agatactctt
    32881 ctttcctacc atattttgtt ctaattgttc cattctggtc aaattctcct aaatcaaaga
    32941 gatcataaac accatatcct acatcatcac tgctagttcc cttaaatgct ggtggtaacc
    33001 agattttact aattccaagt tttttaaggt ctgatataga ctcctctaat ttcttccagt
    33061 gattcccatc actaggtaaa taccactcaa aagcttgcat tatcaattca tttgtcatat
    33121 tacacctcta aaaaaagatt aggaagctaa gctaccctaa tcctctcaat atatcatgtt
    33181 aagtatcaga aagttaagcg ctaagctact accattctac cactattttt caaataacat
    33241 actctaacac taattatttg ttgtgccacg tttaacaatc ccgtgattaa gcactatttc
    33301 tttttcttcc aactcttctt tgtgcataat ttttgttagc atacgcatgc taacagcacc
    33361 caaatcatag accggttgac tgatagaagt taagttagga cgtgtatatt gtgcgattgg
    33421 cgagtcattg cttgtgataa tttcaaaatc ttcaggaaca cgtttacctg cctcaaaaag
    33481 accatttaac aaacctgccg ctaactcatc ctcagcaaca tatgctgctg ttgctccagc
    33541 attaatcaca cgttgagcta atgcaaagcc ttcagcataa cggtagttgg cttcaaaaac
    33601 caacccctct ttaaagttta agccattttt cttgagacct tctttgtatc ctgccaaacg
    33661 aaccttgcca ttaatatcgt caataagtgg tcctgataca aaagcaatgt ctttatgatt
    33721 gccagctaaa atatcaataa catcaactgc tgctgctttg taatcgatat taacacttgg
    33781 taattgatgc tcaaggtcca cagtacctgc taaaacaatc ggtgtgcgtg aacgagagaa
    33841 ctctgcacga atcttttcag ttaagtggtg tcccataaaa ataataccgt ctacttgctt
    33901 agcaaacaaa gtattaacaa cattaacctc tttatcatcg tcttcatcac ttgaagcaag
    33961 gacaatgtta tatttgtaca ttgctgcaat atcatcaata ccacgcgcta aaattgaaaa
    34021 gtagctattt gcgatattag gaatgacaac accaacagtt gtcgtttttt tacttgctaa
    34081 accacgcgcc acagcatttg gacgatagtc aagacgatca ataacttcaa ggactttctt
    34141 acgtgtattt tccttgacat ttttatttcc atttaccaca cgactaacag ttgccatcga
    34201 aacacctgct tcacgggcaa catcataaat cgtaatcgta tcatctgtat tcatattgat
    34261 ttaacttcct ttctatttga aaatgacgct ttcactgata ttttatcacc ttttggaaac
    34321 actttcaagc ttttataaag cttttttatg aaaaactttc aaaatactaa ctaaacttga
    34381 ttttttccta tttttttgga aaaataatag gcataagaaa ctagcctaac tagactaagt
    34441 tccttttagg aagaagggtt attatgtcaa aacttaatcg tatacgccat cacttacatt
    34501 cagtccaagc tgaacttgct gtcttttctg atcctgtgac agtcaactat ttaacaggtt
    34561 tcttttgcga tccccatgaa agacaaatgt ttttatttgt ctatgaagat cgcgatccta
    34621 ttttatttgt accagcacta gaagtatcgc gtgcaaaaca aagtgtacca tttcccgtct
    34681 ttggttacat agattcagaa aatccttggc aaaaaatagc tagcaactta ccatcctttt
    34741 ctgtttctaa agttctagcc gaatttgata atcttaatgt tactaaattc caaggtctcc
    34801 aaaccgtttt tgatggtcat tttgaaaacc taacacctta tattcaaaac atgcgcctaa
    34861 ttaaatcacg agatgaaatt gagaaaatgt tagtcgctgg agaatttgct gataaagctg
    34921 ttcaagttgg atttgacaat atctcgctaa acaacactga aaccgatatt atcgctcaaa
    34981 tcgaatttga aatgaaaaaa caaggtatca ataaaatgag tttcgacact atggttttga
    35041 ctggcaataa tgcagcaaat ccacatggta tcccaggaac taataaaatc gaaaataatg
    35101 ctttgttatt atttgattta ggtgtagaaa cactaggtta tacgtcagat atgacccgta
    35161 cagtagcagt ggggaaacct gatcaattca aaaaagatat ttaccattta tgtcttgaag
    35221 cacatcaagc ggctattgat tttattaagc caggagtcct tgcttctgaa gttgacgcag
    35281 cagcaagaaa tgttattgaa aaggctggtt atggacaata ctttaaccac cgccttggtc
    35341 atggattagg tatggatgtc catgaatttc catctattat ggctggtaac gatatggaga
    35401 ttcaagaagg catgtgcttc tctgttgaac caggtattta tataccagat aaagttggtg
    35461 tgcgaattga agactgcggt tatgtgacta aaaccggctt tgaagtattt accaaaacac
    35521 ccaaggaact actctatttt gaaggataaa aaaagagctt taagctcttt ttttatgtta
    35581 cataaaagta catcatcttg tttctattag cattggcgcc ataaatgtgt atcaattaaa
    35641 ccacagtaag catctactgg tgaaactcct gtaaattctg attctccagc caatttcgca
    35701 actactgcac ttatcgtgct aggaagccca tcataagcat taatataagt ccctacctgt
    35761 ggcatatctg ctaaagcaaa tgcatgttgt actgaaatca caatagatgg tatttcatga
    35821 acgtaaaaag gctgatcagg ggtgccttta gctgctggcc aaataatacg ttgtgttgtt
    35881 cctccagcat tgacatctac caagttaata atcaagtcat agtgttctgt tagatttgcg
    35941 attggctgtt tttgtgcgta aacattagcg atagctgctg cccgctcctc ttgtggcaat
    36001 ttcataatac gctcttcggt agactcccag acagttactt catggccacg tgcctcaagt
    36061 aactctttca tgtaatccga tgcacgttgc ttgtttccag caatcatcgc accaaaacct
    36121 cccttatatc cttcaacgtt aacaagtaag atatgacgat aacgttctgg atttactgga
    36181 aagatatctt tttgtttatc ttttaccaga gttactgctt tatcagcaac ttcatcagct
    36241 attttttgcg actctaatgt gttgattaaa gccatagcct tatctttagg cataaagagt
    36301 tcttggcgac gtccctcata gttatgcaag cctaatttcg cttttagacc gagagtacga
    36361 cgtaaagcgt catggagacg ttcctctgtt agaatacctt tttcgtaacc ttctttcatc
    36421 cattgaatgt cttcatcagg gtcattaaag aataagaata aatcacaacc tgcttcaatc
    36481 gccgttggca acaaatctct ccttggcatt gaagcagtca ttccaaccat atgagaagcg
    36541 tccgttacaa tagctccatt ataacctaac tctcctctca ataactcatc taaaagtgtt
    36601 ttattcaatg aagctggtaa catatcatca aggtctcgct ctggatgcat ttctttttca
    36661 acgtttggta aatgtatgtg acctgccatg actcctggta aacctgcgtc agccaattca
    36721 ccataaatgc ggccaaatgt cgacatccac tcctctttac tcataggatt ggaagcaaat
    36781 gacaaatgat gatcacgctc atcaatgcca tcccctggaa aatgtttagc gaaaggtaca
    36841 atattatatt gcatgattcc tttcatatac tcttttgaaa gtgaaataat ttgatcaacg
    36901 ttagcacccc aatttcgact agctatgata ggattacgcc aattacgtgt caaatctaca
    36961 attggagaaa atgaagcatt acaacccaca gcagatgcct ccatccctgc gattcttccc
    37021 atttcataag catatttcgg atcatttgta gcagccactt ttatttcatc gccaacttta
    37081 gttccatccg tgacagcacc gtctccacca gcttctgtat ttgcagcaat caacatcggc
    37141 aatttcgact ttgtctgtaa aattaaattc tgatcataga tttcacttga actacctcta
    37201 ttatagcgaa cagcagcaat atgataccga tccaaaacat ctgttaaata ttcttctgaa
    37261 cgactagctc ccatattgaa aaaaagttgc ccaattttct catccaatgt catctcatta
    37321 attgtttttt caatccactc aattgcttct tgatttagat tgaagggttt ctttgttaag
    37381 tcaactaaat gtgtcatagc ctatcctttc tattagttat ctaaattagc ccaaaaggta
    37441 ttaatagatt ctttttgcct ttgctgttcg aattgacctt ttgcaaaagc ttcaccaata
    37501 tcttctctaa gaatttccca tgatttgact tccttaccaa caagtactgg gtaaaatgca
    37561 ataccatttt gttctgctgc agccagatca cccagggaat ctcctaccat caaaatagtt
    37621 tcacggcagt aaccacttgc aattaaagtg gcgataacat cttctttttt acctctatct
    37681 tgacaataga tatcatcaac atactttagc aatccctgtt cttcccactc ttgccaaaca
    37741 gcttccttgt tagcagaact cacgacataa acattaccaa attgacttaa tcgttctaaa
    37801 ctttctaaca cacctgaaaa cgctaaagaa tctccctcat aatttttgat ttggcgattc
    37861 acttcattgg accattctaa agccttctct aaatctctac taaaatcgtt ggcaagcgct
    37921 aactctaagg aattattgga caatgagtct gttgtttcga cccattcttt taatttatca
    37981 atatgttcta ctccggcaaa ctcaaggccc ataaccaatc ccacaaaccg attaactcca
    38041 cgagttcttg aataaagatt gactctatcc cactctttta aaaaacttgt tctttcttgt
    38101 tctgatatcc caaatatatc tgcagcaatt ggaccaaaaa agagttgatg cttatatgtc
    38161 attgtatcca ttgcgcaacc atcagaatct atgcaaaaag cgattttgtg atttgtatct
    38221 accatattat ctccagtttc taaatttatc taacttatga ggattttcca attagcaacc
    38281 tacccatcat ttagtggtaa gatcattaaa caaatttcct taaaaaacca ctcaactcaa
    38341 taatctctta tgtgttaagt ggctattatc ttgtaaatta aacaccagag taagcagcaa
    38401 atccaccatc aatcggaagg acaacgccat taacaaaact tgatgatttt tcatctgcta
    38461 aaaagaacaa tccgccaatc agctcacttg cttctccaaa tcttcccatt ggtgtattat
    38521 taagaatttt ctcagcacgc gcagttggct gaccgtcctc agtaaataac aagcttcggt
    38581 tctgatttgt taccaagaaa ccaggtgcaa tagcattaca acggatacca acttttgaga
    38641 aatgaacagc tagccattgt gtgaagttac taatagctgc tttagctcca gaataagctg
    38701 gtattttggt cagaggggtg aaagcattca ttgaagagat attaataata ttcgctcctt
    38761 cacgtccaac catatcttgg gcaaatactt gcgttggcaa taatgttcct aaataattta
    38821 agttaaaaac aaagctaata ccagcttcat ctaactcaaa gaaggttttt gtttctgaag
    38881 gtaagtctaa ttcgtgaaat tcattatcag ttgtagcttt aggattgttg cctcctgcac
    38941 cattaactaa aatatcagtt ggtccaagat cctctaaaac ggcttgatgc acttcttcaa
    39001 ggttttcttt agacaaaacg ttagctttat aagccttagc gataccacct tcttctacaa
    39061 tttcatcagc aaatgtttga gcagcttctt gattcaaatc taataatgcc actttagcgc
    39121 ctgctttcgc aaattcttta gccatataac cacagagaac accgcctgca cctgttatga
    39181 cgacaacttt attggtaaat gtaattgttt ttgacatctt attcctctca ttaacttcta
    39241 taagtattct actcttagct aagtacaatc atattatgat aataactttc caaatttctc
    39301 tttcaggaga ttggaacagg agaaatttgg aagcatcgcc ttattttgaa cctttaatca
    39361 cagcttcgta taaaccagaa acgtaagtgg ctcctaaagc tctatcgtat aggccatatc
    39421 ctggacgacc tgtttctccc caaatcatac gcccgtggtc aggtcgaata gcaccctcaa
    39481 acccaaattc atggcataac ttaataactt catacatgtc tattgacccg tattctgatg
    39541 gatgagccga ttctttaaat gactttccat cacctaattt aatattgcga gcatgaacaa
    39601 agtttacacg atctaactca aaagcacgtc gcgaaatctc taaaacatca ttctggggat
    39661 cagaagcgta agaacccaca cataaagtga tgccattact tttactatcg tacaatttca
    39721 caaaacgctc aatagcctct aacccagtaa taatgcgagg taaaccaaaa atagaatatg
    39781 gtgggtcatc cggatgaatg gccattttaa cgcctacggc ttccgcttca ggaataatac
    39841 gtttaataaa ataagtgaga ttttcccaaa gtttttcctc atcaatttca gcataggcat
    39901 ccatgatagc cttcatttct tctttactat agctggcatc ccatcctggc aaagataatt
    39961 caccgtttac tggatccatt tttttagaaa ccgtctcatc aaatatcaaa gctgtggaac
    40021 catctggata ttgatatgct aaatcagtgc gtgtccagtc aaatactggc ataaaattat
    40081 aacaaatagt atcaatacct tccgcagcca aatttttgac ggtttgaata taattatcaa
    40141 tcaagagatc acgtgttgga cgtcccaatt tgatatcttc atgaacaggt actgattcga
    40201 taaccgaaat tttaagccct gctgcctcta ccttttcttt cagctgttga atgcgctcac
    40261 gtgaccaaac ttctcctact ggtacatcgt atatcgctgt aacaatccct ttcattgttg
    40321 gaatttgacc aatattttct aaagtaacgg ggtcgtcttc accataccac ctaaatgaca
    40381 tttccatgat ttcactcttc ctttttagtt cttaaaataa ttaactgcat tttgataagc
    40441 gatgtcttta gccatacttc ctaaagcttg ataatcttct ggaacttctc cttcttcaat
    40501 ccattctcct aagtaggtac ataaaatacg tcgaaaatag tcatgacgtt gataagatag
    40561 aaaacttcta gaatctgtta acataccaat aaaattcgcc aacatgcctt gctctgctaa
    40621 ggcattcatt tgactaatca tccctaattt agtatctgca aaccaccaac ctgcaccaaa
    40681 ttgaaggtaa gatctcactc caagttcgtt agcttgaaaa tttgccaaag tgttagctac
    40741 tgctatattg taagctggat tgagattata ccaaatcatt ttaggcagac tatctttttt
    40801 aaccaaggaa tccaataaac ggttcatatt aacagttaaa gccacttggt caccaagtga
    40861 atccacaccc acgtcagctc ccaatttttc aaaaatagta gaatgattat ttctcaaagc
    40921 tccaaaatgc acctgtgtta caaaaccata ttttttatat aatcggcaaa gttccataaa
    40981 aacagctgtt tgccatttgc tgatttctga ttgatttggg atatagcctt cacaaacttt
    41041 attaaataaa tcgttcaact ccaattcatc agtttgttca aagacgattt ccgtaaaact
    41101 gatatcgcta gccctacaac cattttgagc aaaatacgca atgcgttctt ccattgctgc
    41161 tataaatgtt gaaaaatctg taatctcttt ttgagtaatg tcacccagtt tagtaataaa
    41221 atcaacaaaa tgtcgatgtt caataaaagc ttcatctggc ctaaatgtcg gagcaacaac
    41281 tgttttaaaa ctctcatcag cagccaactt tttatgccac tcgagagtat caagagggtg
    41341 gtctgtagta ccaataaaca taactttgct atctgcaatt aaacgtctag gacttatttt
    41401 atgctctttc aagaaatgat ttaaacgatg ataaatttcc tcagcatttg attctgtcaa
    41461 aatctcatta acaccaaaga tatttttcaa ttccatagca gaccaatgat aaacaggatt
    41521 accgtatgct ctttctagtg ttctagcaaa tgctttaaat ttctcaaggt tggatgctgg
    41581 acctgtaatt tctgcttcag aaataccatt agccctcata agacgccatt tatagtggtc
    41641 accaccaagc cacaaatcca caatattatc aaaaatatga tcttcaaaaa tgtcctttgg
    41701 atctaagtgg caatgataat caaaaatagg ttgtctcttg acctcttcat acagctgtat
    41761 agctgcttga ttttttaaca taaatgtttc agtattaaac gccatattct tctcttactc
    41821 actaatcaag tgcagaaaca aactgtttag ctattttagt aacactatca tagccttctg
    41881 tggctacttt actagataat gctgagccaa caccaaccgc aactgctcct gcttttctcc
    41941 attcaactac atttgaaaca gagacgccac ctgatggcat taaatcaaca tctggaatag
    42001 gaccatgaat atctttgata aaacctgaac ccacaactcc acctggaaaa agctttataa
    42061 tctgacattt atactttcta gctaccacga tttctgttgc cgttgcacag cctgggaaat
    42121 aataaacctt gttttcatta gctaaattag caatatctga atcaaagtgt gggctaacca
    42181 aaaatttagc tccagcatca atagcctctt tagcaagttc agttgtcatg accgttccgg
    42241 cccccacaat aatttcttta ttatttttaa agtcatctga taattgtttg atgactttgc
    42301 ttgcttcagg agtcgaaaaa gttacttcaa tattccgaat accacccaaa atagcatgct
    42361 ttgcgatttc gagcgcatcc tcagatgatt tccctcgaat aacagcaaag aaatagttgt
    42421 ttttcaattg atttaacata atacctccca aaatgtaacc gttatcttta actatattat
    42481 ataagtcatc tgatgaattg tcaagaagaa tactaaaaaa atagctattc aagaatagct
    42541 atttttcttc ttatattaag ctttcaacgc tgaacgtcta acactcatga tatgcataag
    42601 catagcatca tgtgcagcta ctggattttt ttctcggatt gctttgataa tttctctatg
    42661 tgcttctaaa ctatccgact tcatctgacg atttgtataa ttcgcattta tcagatgaat
    42721 tgactggttg atgactggca aaaggctatc cattgcaata ttaccactgt atttagctag
    42781 catggaatga aattccacgt ctaaaagtaa atgtttcgga tcgcctgcgt gaacagcatc
    42841 ttcaatatct ccaacaagct tttcgagtaa ttgtaactga tcatctgtaa ttctataagc
    42901 taccagctct gctattcgag gttccaataa aaggcgtagt tcaaagagat ctgatgtcaa
    42961 cctatctgtg tcctttatta gagagaaccc taatggatcc tcagaaacac cctttttaga
    43021 actaatatat gttccagagc cctgtctgac ttctaaaata ttacgcgttg ctaaacttct
    43081 gactgcttct cggattgtgc tgcgaccaac atctagatct tctgctaatt catattcatt
    43141 ggggagctta gcaccaacag ggtattctct ttctaaaatt aaatgcaata gacgatctgc
    43201 tgtttgttct actaagggtc tagccatatc atcacctctt ctaaaataac tacccctatt
    43261 ttacactatt ttttttatta tggtaatgag gaatagtcat ccagcgtttt ttaaattctt
    43321 tgacgacttg tttcggctgg cgattccttg aaaagagacc tttgtgattc ccctgtacac
    43381 gaagtatcat cagattagtt tcaaagtctg caaaattcca gacttgctca ccaactaaat
    43441 taggaatacc atcaaaaaca cgatgactca tttcataaaa atcacattga aattcttctg
    43501 tatatggaat attccaagta gaatgtaatc ccggtaacgt atcagcgcca tactccgtta
    43561 tgataattgg tttgtcagga aatttatctt gccattctaa taactctttt cttataccta
    43621 cttctgcatt tgttaaatca ccgtggtcaa cgtaccaacc atagtaacga ttaaggcaga
    43681 caacatcaac caggtccatc acttggtctc tatctggggt tgccattaaa atattaacca
    43741 aggtgacagg acgtttttga gggtctaaat ctttataaag ttttactaat ggctcaaaat
    43801 aatcatgtgc tcccgcttca tgactagccg gttcgttagc gactacccac atcacgacgc
    43861 taggatgatt tttatcacgc ttcactaatt cttggatagc ttgttcatgc gccgcttttg
    43921 tttgcatcaa attccacgta ccattatctt ttggtgacaa atctaaggaa gcgttaaaat
    43981 tttgaaataa ccctactgct ggaacctcat ctatcactaa cactcccatt cgatctgcta
    44041 aacgcatcat ttcttcagaa taaggataat gggatgttct aaaagagttt gcccccatat
    44101 ccttcaaaag attcaaatcc attaagttag cagcttcatt caaacctctg ccattaatga
    44161 acgtatcttc atgtttacca aatcctttaa aataaatagg tttgcgattc aataggaatt
    44221 gaccatttgt cacttctatt tctctaagac caaaattttc ctcatagaca tcttggagtt
    44281 gattatcaac aaaaatttca acacgcgctg tgtacagata ggcatttaaa acttcccaaa
    44341 gatggacatc actaagaaat aatctgctat ccttcgtttc acccactagt ttgttatctt
    44401 catcaaaaac gctaattcta acttcgtcaa ccttttgatt agtctccact aaaaaatgta
    44461 aatctgctga ctgtaaatca tctgatagac gacttgtaat tgtaatatca aaaatatgat
    44521 ttttaggacg tatcatcaac ttaagaggac gatgcacacc tgcataattg aagaaatcaa
    44581 aattctcacg tactttcttc ttaatgctac catcttcttg aatgatttca ctataatttc
    44641 cgaccggaag cgtggtatag tctaaaacgt tattagcaca aattgatacc ttaattttct
    44701 cattattata cttacattca ggaacaagaa cttcaaaagg agtaaaacct cccttatgct
    44761 ctcctaccaa tacaccgtca acataaattt tagcttgatg tgtcactgag ccaaaacgca
    44821 gcaccatctc ttcatcctct gatactttag gtacttcaat gactttttca taccaaaaat
    44881 cacctatata atcacgtttt tctttactta ccatcaaatc attaaatgaa gtcgggataa
    44941 ccatgacttc atctgaaggt agtaattcat ttggattatg ttctcctaat ttaaagttcc
    45001 aaataccgcc taaatcataa gtatttcttg tttttgtcaa taatggatat aacatacact
    45061 ctccttttac actatcgttt aatatcattt gtttcttttt caatgaggta agcaatatca
    45121 tctcgtgtca tgatattgat atctccttga atggtatgtt tgtatttaaa tgtagccata
    45181 gctatttcta aggccctttg aggagtttct ttttccagaa gcgcatgaat taaacctgca
    45241 gcaaaggcat ctcctgtccc cacacgatct aatacttcaa cgccagtttt atcagtttga
    45301 tataacatat tattatgata caaatatgat tttaacatat actgattagt atattccatt
    45361 tccctttgag taaaggcaat atagtctaac ccatattctt ggacaatctt ttgggttatc
    45421 ttttcaagta attcaatatc aaggtaaggt ctggataagc ccaactcatc ttttaaatct
    45481 tcactttcgc cagctaaata aatcggctct aagccaaagc aaacatctaa taatcctaat
    45541 agtggcgata attgctcacg tgcctcttga aaagatgacc ataaactctc acgaaaattg
    45601 agatcaatcg atacttttac acctccttct ttagcttttg tcataaggaa ccgagtcacc
    45661 tcatataagt ccttggttag tgctggagta attccagaaa catgaaacca atccactccc
    45721 ttaaaaatag aagtaaaatc ataatcactc aaggtacttt cccaaaaaga tgaatagtta
    45781 cgatcgtaga taaccttatt tgttcttacc gaaaatcctt gttcatagta ataaattcct
    45841 agtcgtcctt cttttttaat tatagcagct ggactaattt gctgtgacaa tataaattga
    45901 cttgccatct tgcccaaatc attatcaggc aaagctgaga ccaatcctac atggtaacct
    45961 agttgagcaa ggcttgctaa gacattaagt tctgaaccac cgaattggca ttttaggtga
    46021 ttagcctgca tcagtgtgtg atattgaggt ggagataatc gcaaaagaac ctcacctaat
    46081 gaaataattt ttgccataag aatcctccaa tggtagctct tatttaacaa aatagctgct
    46141 ctattgaaga gcagctaaac tttttaatct ttatcaatag gatattttgt tacatcaaca
    46201 taatccatat ctgtcaaagg aacattacga gcaatatctt tagcacgttc cttagtagtt
    46261 gctgctttca taacttgaat cttctcttga atacgaacca tttcttcttt atctagtttg
    46321 taaaatttca ttaaaagaag agctatagac aaagctataa atggaatggc cacaaggata
    46381 ctaatagctg ccatttttaa atcagggggc agtggtgttt cgatagttgg aaatgatttt
    46441 gaaaatccaa ttcctgctaa cacaaaacct actaccatag gagcaaatga agaagcaatc
    46501 gaatctgtta aggagaagat agttccaatc attcctgata cataacgacc ggattcagaa
    46561 gtttcatagt cagaaatatc tgctcccata gtcaatacta aactagcagg tgcttgtgaa
    46621 gcgtatctcg caataatatt agtaacgata aataaaatag tataaagatt taggcttgtt
    46681 aaactaaggt caccaggctt accaacgtaa agaactgcac caaaagcaag taaaccaatc
    46741 ataccgatct gaattgctct gacatatgaa aaacgaagac cttttttacg agcaattgtt
    46801 gagaacaaaa tattaataat aacaccagga acaatgaaaa gaagtgagaa ttgtcctgat
    46861 aaagcataat taccaaataa aataccaaac aatagcacca taacaacgga atcgccaaag
    46921 aattggatgg caaattttac caaagctgca gcaatggata atatctgaag cggtttattt
    46981 cctttcaaca ccttccagta atcttttaga gcagtctttt gggtattctc tcctaaacca
    47041 aagaattctt ttctgtcttt tgcccaaatc ccaataattg caacgattgc taaaatagct
    47101 gaaataagaa ttgttccaaa aataaggaca ttgaagaatt gaggtgtaaa attaccaaat
    47161 tttggtacta agaaaactga aacgacaaac tgaccgcctg tcattaatga tgttgtcata
    47221 actgcatcaa cgatattaaa aataggacgt tgcttagggt catttgtcag tgctgtttgc
    47281 cctgctttag ttattgtctg ttgcattgag tatccaattt tatggataat tagaacaagg
    47341 ataaataatg ggaaacgaat attttcatcg acacctctca atgcaagaag aaaaatcaat
    47401 gacaaagccg taataacgtt cccaataatc aaaataggac gatacttacc aaattttgta
    47461 tcagttttat caatcataat accaatagct ggatcaataa aaccatcaaa aatacggata
    47521 taacccataa tttgactgac aaaaatggct gccgagccaa gaacgccagt tgagaaatag
    47581 gtaacaaaca taaacgtaaa taagtatata tttgtagaag cattgttcat ggcaaacata
    47641 actaactgcc atactttcgc tcggttataa gatacaactt cttcacgttg ttccatatcc
    47701 attgttttct ccatgagaga accctccttg tattttgtaa acgcttttat gattaaataa
    47761 tactattcat ctgatgtttt tttcaatatt tttataaaaa atatcttttt tattataaaa
    47821 aagactttcc agctataaat acataagatg tttttttgct ttaaaggtca taatcatcat
    47881 atgttttgtt accccacaaa aaaagatgcc taagcatctt ttagttgact ttcgtagtag
    47941 ttgttcccgt ttcaatcact tctacacaag catttaaggc accctctact agatttttga
    48001 ccgcttcatc tgtataataa gccgcatgag gagtgtatat tactttcgga tggttaatca
    48061 gtcttaggaa atccttgtct gaaatgtctt gaccttgaca atttttaggt atatatggcc
    48121 cctcaaattc ataagtatca ataccagctc cctccaaaag cccttgatct aaagcctcaa
    48181 gaagatcttt ggtttccacc aatgctccac gtgccatatt cattaaaata gcaccttttt
    48241 taaactgttt aaacatatct aaattaaata gatgcgtatt ttcagcagtt ggtggcatat
    48301 gtaaggacac aagatcagct tcttcaacag cttcttctac cgaattgaca tactcaagga
    48361 taccatctgc cattggatta tggtaaatat cataaccaat aacacgacat ccgaaccctt
    48421 taaaaatttt agcagtcgct agaccaatgc gaccagttcc tattatagcc acagtcatgt
    48481 tacctaggac tcgtcctcga attggcaagg tccatgaaaa attctgttca cgaacattag
    48541 ctctaataag ctcaactttt cgaataaggt taagagcgat tgtcactgta aattctgcaa
    48601 tagactccgg tgagtaactt ggcacattgc taatgataat accatgttgc ttagctaact
    48661 caagattata catatcaaca ccagcactgc gctgagcgat ttgcttaatt cccatttctt
    48721 tgagaagagg ataaatagca tcatctaaag gctcaatctg tgcattagca atcccatcaa
    48781 aaccttctac ctcattaaca gtttccaatg tcaaaggtcc ttcactcata gaaagttcca
    48841 catgatttcg gtttgcccaa tcttgtgcta atgttgcttc ttcttctcta acattaaata
    48901 ctttgagttt catcactaat atctccttaa atatctaatt tatagtccat tggatcaata
    48961 attttaagta cttttataaa gaggtaatta aaaataaaat tcaatataat tggtgcagca
    49021 acaaatatta taattatcaa taacatattc ataactgacc atccaccttt tgctaaatta
    49081 agagcattaa tcggtccaat caaaccactg ataccaaaac ctgcactagc tggtgttcct
    49141 tgaatattaa agagagctcc gagaattcct agtattgctg ctgaagatat cataggtagc
    49201 ataatcttcg gttttgctaa tacattagcc atcgaaatct taggagaccc taaaacatgc
    49261 gctaaggctg tccccttaga attgactgac cacccagcca tacaaagccc aaaactagca
    49321 gcacagatac ctaaattagc agcaccagaa ccaattccgg acaaactaat tgccaaagca
    49381 atcccaactg ttgtgattgg agatacgatc aagaaacaga aaatcatcgc aattaaaatt
    49441 gacatcaaaa caggttgtaa accaagtaat gaagcaatcc cttgtccaat catggtggta
    49501 atcattttga cataaggtaa taacacatga cctactcctc caactagtaa gagtgttact
    49561 gttggaatga caatcaaagt gaatgatttc gtcttgtcag ataaaaattg gatcaaaagg
    49621 acacctaagg cagcagtaat tcccatgtta atgatatcgc ctgttccttt tagcataata
    49681 gcgcctttaa ggaaggttgc agctccacct gcaaacataa ccgctaatcc tagtgatgct
    49741 gattgaatgg gattaaattt aaaattgaga ccaaccataa taccaataac aagccccatc
    49801 atacttgttg caactgctgt tgcagcgatt aaagttgcaa agccgctcca cattggcagt
    49861 aaagctttca ttaattctcc aaggattgct cctggtatca agacaataac agtccctaaa
    49921 gctaaaccat ttaatacgtt cattataaac gatttagttg tttgtttttc gacaacatcc
    49981 gacataagat gcctcctact aataatgatt gtttgtgatt tcactaacta tattaactat
    50041 atcatagcta tttgcaaaag gaaaattgga aaaaattata taagtatagc ttttactaat
    50101 attattgtgg aaataatcat aaaggtcaga ttgatagact tgtgctatac tattactaag
    50161 aaaaaaggac agatattatg gatattagac aacttactta ctttattgct gttgcagaag
    50221 caaaaaatta ttcacgcgct gcaaaaagtc tttttgtgac ccaacctaca ctttcacaat
    50281 ccattaaaaa attagaagca gaattaaata ctattttatt cttacaaaat ggtcgtcaac
    50341 tcgctttaac tgaagcagga gaaattctct atgagaaagg acaattgttg atgactaatg
    50401 ttaatcaaat ggttacggag atccagcaat taaatcaaga aaaaaaggaa ggtatccgtg
    50461 tagggcttac ttctcttttt gctattcaat ttatgaaaca gatttcaact tttatggcta
    50521 cacattcaaa tgttgaagta tctttgattc aagatggttc ccgtaaatta caagaattgt
    50581 tagcaaaagg aaagatcgat attggcttat tgtcctttcc aagtacccgc aatgatatca
    50641 ctatcgaacc actgcaaacc tcaactaaag gatataaagt aagcattgtc atgccaaaat
    50701 cgcatcctct tgcaacactt cctgaaattg aacttaatga tttaagagat tataaagttg
    50761 cctcactcaa cgaacattat atgctaggag agatgttgcc tcgtaaatgt cgttctttag
    50821 gatttgaccc ccatatcgtc tttaaacaca atgactggga agttcttatt catagtttac
    50881 aagacctcaa tgcagtcact attttaccaa gtgaatttga aagcattagc caagttcaag
    50941 atttatgctg ggtcccactt aaggataaaa ataattttta tccgattggt attgcttacc
    51001 gaaatgatac ttcttttagt cctatgattg aagaattttt aagtctttta aagaccaatt
    51061 aatagcagtt aaaccaaaaa gttttaaact ttttggtttt atttattttc caaatgacaa
    51121 agaaaatcat aaactttttg attccactca taaagtgttt cgttacagta atctaaataa
    51181 taaaccctgt gtttcaagtc attgggcaca aggctttgat aatcttcttg ataatgtttt
    51241 gggataacac atgcaactaa atgttctgct aaagaaagct gatttttaat tttagcagga
    51301 tgataaccat caaaaataga atcaggatga ttgatgatat cttgataagt aaaatgaaaa
    51361 ctgacccctg gcttaaaacc tactgttaac tcctgttcag atggtgcttt gcctaacaat
    51421 ctttccatta ctaaacgata accagaattg gtatttgacc agttcaacat cacatagtca
    51481 aagtaatctt tagggtctcc agcagcattc ctcttatctt ttaccagtac ttcagcaggc
    51541 ttattaaagg ctttaactgc tgataagatt ttaccttctt taaaaattag ttttgcagtt
    51601 tcaatcgtac atgtatggct taaaaaatca gaagttaaac taataccatc ctcattaaaa
    51661 gtagctgccg cttctctata tttgggaata tagggaaaag taaaactatt gtattttaaa
    51721 aaatgaacaa tttttgcgag gagatgttta tcgtcacatt gtatctcagt ttttctttta
    51781 tattgtttct cgtaagctat gaaagctcct atcctttcca attcaaacgc cttaattcga
    51841 ggataatcca gcaaagctaa ttggtaaaca cctccccatt gtaaactata tgctgcacga
    51901 caaattttta aaatcatttc ttcctccttt tgataatccc tacaacaatt tctattaaat
    51961 agtagcattt aaacgtaatc gactcaataa cttggtcaat tttcgatcaa aaataagatt
    52021 aagataccaa aaaagcctgg actatactta ccagactaac atactgttta ttaaaatgaa
    52081 tcaatctttt gttactacta attgcttacg attattaaag aaaattttat aagccaaata
    52141 agtagctaca aaagaagcta tcgaagtcgt tgctaatagc ataaacgtca ccatcatttg
    52201 gtatttaacc gcttggatag gtgaagttcc agcaagaatt aaccctgtca tcatccctgg
    52261 caaagataca atcccaagtg ttttagctga atcaattgta ggtaccatac cagttttgat
    52321 aacatcacga ataatatcaa tcgaagctgg caaaatatct gctcctaaag ctaattttgt
    52381 ctccacttcc tcttgtttac ttctaaattc ggacaataat tgtttatagc acaaaccgat
    52441 tgctaccata gaattactaa taatcatacc acctacagga atcatttgat tcggaacaaa
    52501 ttttaaaatt cctgaaaaaa tcaagacaga taaagtgatt atagtccctg acccaatagc
    52561 tataaatgaa ataacaaacc ctttattaat ccctttacca cgcttagctg cgttataaga
    52621 agcattaata atcatgaata gcattaaaag agctgtgaaa ataggatttt gataaccaaa
    52681 tatatagtct agcaaaaaac ccactgctaa taattgaatc actgctcgga tagcgctaat
    52741 aatcacttct ttttctaggt ttaacttttg ccagtaagaa aaaaataaag taatcaaaac
    52801 taatgacgat gccattagta atgacattaa agaaatagaa tttgcaccgc tcattttata
    52861 acctccattg cttcaatact accattgaca acttttaaga ctttattggc aaatcgacga
    52921 ctctgttcct catcatgcgt aatccacaaa atcgttattc ctttgtcatg taatgattta
    52981 ataacctttt caacaatagc tttattatga ttgtctaaag ccgaagtaac ttcatctaag
    53041 agtaaaactt taggttcaaa caataattgc ctaatcagag caatacgttg cttttctcct
    53101 cctgatagtt ttttgacatc ctgtttcagg taagactggt ccatttcaaa acgttgaaat
    53161 aaatcatcaa cgcgtctata atctactttt tcgtgtcgaa tgtggtaagg aaaactaata
    53221 ttgtcttcca ctgtattacc aaataaatgt ggcgtttgaa aacagtaaga aatcattttt
    53281 cgactttcaa tcggttctaa ttggttcaaa tcttttccat caaagaaaat atgacctgca
    53341 gtaggactca ttaaaccact ggctaatttt aatacagtac tcttaccact tccagatggc
    53401 cctacaatag aaacaaaatc accttcatca attgcaaagt tgatatcatt taaaaccaac
    53461 ttatcatccg ttttaaaatc aacgtgttta aaggttaaat aggacattgt taagctcctt
    53521 tatttcttat ttataatcat tataattaag tctaacataa aaatcaaaaa aaagctcttt
    53581 agaataattc taaattaata agtaaactta ttttacctct tattaaaaat tttctgataa
    53641 aataggcttt gtaagaggcc ttataaacaa gatagttatc atccaagtct ctgcacttta
    53701 gttgatcaat aaaggagact cctatgtttt taatcgattt tattttacat attgatactc
    53761 atatctatgc tatggctaac actgttggta attggaccta tctattgctc ttcctcgtta
    53821 ttttcgttga aactggggct gtcatctttc catttttacc aggtgatagt ttattatttg
    53881 ctgctggagc ccttgctgca aatccaaaaa tgtccttcaa tattgtaact tttcttatta
    53941 ttttctttat tgcagcattt attggtgata gttgcaactt cttgattggt cgtacatttg
    54001 gttaccgttt tatcaaacat cctttctttc gtcgtttcat taaagaaaaa aatatccgag
    54061 atgctgagct ttattttgag aaaaaaggta ctgctgccat cattttaggg cgctatatcc
    54121 ctattatcag aacctttgtt ccttttgtag caggtattag tcagctccct ccaaaagtat
    54181 ttatcaaacg cgccttcatt gcagcattat cttggtcagt tattgcaaca ggttctggtt
    54241 ttctctttgg taatattcct tttgtgaagc agcacttctc gcttattatc ttgggaattg
    54301 tatttgttac cttaattcct gtccttattt ctggtgtcaa atcatatcgg aacactaagt
    54361 aatgtatcca aaaatttcta tttaacacta ttaatcacta agaatgacaa caaaaaaaga
    54421 gcaattagtt gctcttttat taatttagtg ttatttctcc tgttgcataa tccatagtgt
    54481 atgatgcttg tgtgtttgga atagcaacat taaattctaa tgtgccatct tgtgatttag
    54541 cttctaggtg cattgcaaat ggaactaaat cagtatcatt acggtacaat ggagttacac
    54601 ggtaacgaac acgtttgttt tggtcaaccg ccttacgaac taagctttca taataatttt
    54661 gtccacgatt gattttttga tttgattggt tggaatgagc tgtttgtgtg acaacatttt
    54721 gaggatttga cacggaagca tcccaacctt tgaaatttcc agctaaagca taggcaatta
    54781 aatgcccctt gtcgactgca tgcccataat ggtcattagt agctacttga tgccaaccta
    54841 atggtttcca atttgcatcg ggaagagtgg tatctctgcg attttgatat tgacgaatgg
    54901 ttctatcaag cattgcatta gcagttgata gtctttgttg attgtcaatt gtattttgaa
    54961 catacgcttt aacattgaca tcagcattta aatttgtctg attattattt aattcataaa
    55021 taccattaac atattgaaat ggtaggttac caatagcatt ttttaaatta ttttgaaatt
    55081 cctggctcgt atcaacagat gaattgacat cagcatgtac tatcggcgtt acgttttgcg
    55141 gctgcgttgg aagaccaaag gtcattaccc ccaacatacc taatgtagca attacggtta
    55201 taaacagttt atgtaatctt ttcataagct ttacctcttt ctaattacat taaacaagcg
    55261 tctaccatat tgatatccgt tttagctaat tgctctcctt ttgtcacaac catatcatat
    55321 gcctattatt taagcatgtc aatcatttat ttaatcttat attaatttaa tattacttta
    55381 aatttaattt actcgttaaa ttagaaaatc tgcaaataat agctttgttc atcccctaac
    55441 ttaaatcaaa atttatgcta tgatagtggt aaggaggctt gttctgtgaa aaaaacaatt
    55501 ggtattatag gtatgggagt tagtggttta gctgttctac tagcactttc tcgtaaaaaa
    55561 gagaaagagt taaaacagtt agctattttt tgttttgatg atactgtcca ctttggacga
    55621 ggaattcctt ttcaagaaga cgttgactca gctttaatca attccccaat caacgatatt
    55681 tcatttgatt atcgccatat gtctgacttc atggattggt tagaagaaaa ttactatgac
    55741 acaaatctaa cctatgtatc aaggacactt tacggacaat acctttcaga aagaactaag
    55801 ttacttttag ataaactccc tgtcacggtt attaaaacaa gagtggataa actaaactat
    55861 ttatcagatg agcaacaatg gcaagtctat attcaagata agctttttcc tgtcacattt
    55921 gatgaattac acttagcttg tggtgctctg cctgttatag aaccttatca tttggaaggg
    55981 aagaccgatt atatcaaaga cccttatcct atcaaatact tggctcagga aaattggaag
    56041 ggaaaaacag ttgctatcat cgggactggt ctagctgcaa ttgatgttat aaaatggttg
    56101 ctactcaata cacaatcttc tgttaaagca ttttcaagaa ccaactactt ccctagcgtc
    56161 cgtattgtta atgatttacc acttacttgg cactttttta ccaatgataa ccttaaagca
    56221 tttctatcac atccttcaaa aaccttcact attgccgatt ttaatgattt gatgtctggc
    56281 gaattggagc atcttggctt taaaaactgg aaaaatacaa cagaaacttt tttatcccca
    56341 ggaattgatg gtctcaagtt agcctttaaa taccctaagc aactctatct attacaacaa
    56401 ttagcttcac gaatcactgt ctggttaact gacctttggc cactcatgtc attatcagat
    56461 aagaaagagt ataaagcact ctatggaaaa gcgattatta acttgcgaaa tcctatgcct
    56521 gatgaatctg ctaaagtaat tttgaaagct gcacaaaaag ggactttttc tctgatcaat
    56581 aacgtcaaga caattacaaa aaatgacgaa ggattttcaa tagaaaaaga aaaacaaaat
    56641 catatatctg tcgatcgcgt cattaacgct actggttacc agttaaacac taaaaactgg
    56701 acaaaagcca atccacttct aaaatcgctc atcaatcaag aattttgtca aattgacgat
    56761 caaggaggga ttactatttt aactgaaaat gcacaagttt tgtctccaaa atatggtcca
    56821 atgccaggtt tatatgcgca tggtgcactg gttaatggtg tcgtttatca gaataactct
    56881 acaattaaaa ttcaacaaat ggcagagcga gccttttctg ataataaata accatttcta
    56941 aaaaatacct cctcttttat tatttaagaa agggaggtat ttttttatta taaattcctt
    57001 tgttgtaata tagactgtgc tatactttct tttgataatt cttttaagtt atctcctatc
    57061 aattgtaaag cagtatcagc catatgagcg atctctctac tatgtgtcac cataataaca
    57121 catcgttttt ccttatgcgc aaaatcttta agacgcatcg taatatcacg cgcaatgtca
    57181 aaatctaaat ttcccgtagg ttcatcaagt aaaatagctg gtgttcctac aaccaaagct
    57241 ctcgcaatag ctacacgttg ttgttgccct cctgataatc tcaagatatt tcgcgttaac
    57301 atatcttctt ttaaacctaa atccaacaat aattgtttat ctgcagtagg ttttaccaac
    57361 tgaacatttt ccaatggggt tagatagtct aaaaggttat aattttgaaa gaccaaagct
    57421 atattctttt tgcgatgctg agcataacct gcttctttaa tatctttttt gttaaataat
    57481 acttgtccct cagtaggcga atcaagacct gccatcaagg atagaagggt tgttttccct
    57541 gaaccagaat tccctaatat agtataaaat acccctgact gaaaactata attgatattt
    57601 tctaatgttt tattgtcgtt ccccttataa cgataagcaa tattttttaa ttcgagcata
    57661 gatttctctt tcttgagcta tgtattcaat agcagagcat attcatttta accgttaata
    57721 tagatgtcac tattggtcgg tatacttatg gcactttagg acagttttga taagattccc
    57781 cttggtttgt agcgagcaat aattaaacta gtagtaataa tagcaaataa gacgattgct
    57841 aaaccaacta gaattactaa ccagacttcc tcaggcttca cttgtacatt taacgtctta
    57901 atcgtttgca taaacatatc tgtatcacta tcagccccaa gctgcatatt cccaaaccct
    57961 ttcataaagt ctgctctgac agttctagag gcctgataca ctaaggagtc tgctaatttt
    58021 tgaccaataa attgactgat aagtactgat aaagcaaagg ctggaatagc aatcattagt
    58081 acttctactg cgtactgaca atgataattg ctttagactt accaatagct aataaaatac
    58141 ctgtctcatg ggttctacca ccaatccaga atagcagagc catcatcact aggataatag
    58201 atactactaa catgccgcct tgtagagaag taattaattg attcaagaca tctatacttt
    58261 ttgcataact tgttagcaag gttgctgtaa ttgtcagttg atatttattc caatcaatag
    58321 gattgttttt tgataaaact tttaagagtt tctatatcag cattcttttt aggatataca
    58381 gttgtttggc gataaaaggc cttaccttta gaatagccgt ataaatcctt aatcaattgc
    58441 gtatctgaaa aggctgtatt ttctagcatc tcataagggt aatttgattt ttgtaccgtt
    58501 ttacctttat atatgccaac aatagttgct tcaactaatg ttttatcctt actgtttcta
    58561 acggggtctt ttgaaaacat taacttatcg ccaactttaa gatgatttgt cttcgcaaaa
    58621 gcctgatgaa caattataac tcgctgatct ttcggcgtga tatgtcgccc tcccttaaga
    58681 gaaatcatcc ccgctgtgta tttaggatct gtatcggagt aactattacc atgaacttgt
    58741 aaaacttgtt ctttatattg ttctttatca tttggtcgtg taaccatttt ttccgaggtt
    58801 agtccagcat aagccatcaa attattatta acgcgcttaa catttggatt acttgctatg
    58861 ttaactattg ctttttcagg gatttctcca gatccgtaac catcaccaac aaggtcagta
    58921 ctatccgatg aaatatgcaa aattgatcca gtttgccttg aaaatgtctt cttagcagta
    58981 agaccagcat tttttaccgc aataccgcta attaatgtag aagcaactag ccataaacat
    59041 acgaataaag tgatactctt acgcttttta cggctaacat ataaaaagga acgtttaaat
    59101 aaattcatat taacctccta tgacattcgt gacaatattt gctttggttg tagtttaaga
    59161 atagaataac tagataatac aactgcaaat aagcaaatta aagcaccctg agcatagagt
    59221 ctaaagacat ccattactcc aacagaaaca gcgaggttat caattctatc aataattaga
    59281 ctactatttt gtacaatttg tgaactcaca ccaccattaa cttgtgataa aaggccagct
    59341 gcaaatgttt ttccaagaaa gacaccacca ataatagata gtgtgaaggc agcacctgct
    59401 actaacagta cttctatcag ataatgacct atgatttcaa acttagtttt tccgatagcg
    59461 agtaaaattc ccgtctcttt aatgcggtta ttaatccaaa atgttagcat gacaactaat
    59521 attgccatcg ccacgagact aacaataaga atgattcgtc ccactagacg atgcaatgtc
    59581 tcgatattac gataagaatc tccataaaac tctttagctg tttgaacatc taatttcccg
    59641 aaatcaagcg gccattgttt gatgttttgg ataactgtat ccactaaacg tttatcactt
    59701 aagcgcactg caaccgtctg ataacctttt tgtttactaa tcttcctaac caatgttgcc
    59761 tgagcaatca atgtattatc aatattggga ttaaaagtat ttttagactt agcactattc
    59821 gcatcataaa ttcctgcaat agtaacattt tctttgccta atctcaattt gttacccaca
    59881 cttaaatgat tcttttgtgc taacttcaat ggaactagta cttgttttgt attatcggcc
    59941 gctaaatggc ttcctttctt taagaccaat gcttttgtct gaaaagctcg taaatcttct
    60001 gtactttgat aacctgctaa tgtaacttga ttctcttctt tagaagagtt atatggtgat
    60061 acagatttta aacggctacc aacatcaatg cttaaacttg cctctactga ctcaaccatt
    60121 tttgcttttt tcaaagcttc gtatgcttgt tcaggccaat aagccttttc tttggaggtt
    60181 aaataaatgc ttttcccttg ttttgttagg atattttttt ccaaagaatg ctgaatactc
    60241 atcagactga ttaaacatgt gcctatagtt agaaaaacta agaacaaggt tactgttttc
    60301 attttttgac gtttcaaata agctaaactt aatgtccata ggttcactat tttccctcct
    60361 ttttctatct taataatctc tagaataacg atagaagtcg ttagatattc tctatcgact
    60421 tctacgttat ctatcaatta gtgattttat agcactgttt ctaaaaacaa ttatttctca
    60481 aatgttttca taaattggtt agtataatca attacttctt taaggttagt atcataaatt
    60541 tggtgagttc cgtctagtgt atgaatagag acttcttttg gatcttctga taactcctta
    60601 tgttgctctt gccaactgcc tttaattggt atatctaaac tacgtaattg aagccctaag
    60661 tcactttcac gtgctaataa atacatagca ggaattccct ttggaatctt atagttggca
    60721 ctttcgttaa tcatattaga agaattcttc gcttcttctt tcatattaga attattaagt
    60781 atcttatgtc caatagcaaa aaattgtgca ttaatatcgt cggatacttt aggaatttta
    60841 ggtaattctt ttaacactgg agcagcgaaa tcaacttgct taccaccttc catacccggc
    60901 gttgatgtat ctaaacctat taatcctagc acttcctttg gataagtact gatgtattta
    60961 agggcataaa caccaccaat tgaatgagag gcaattacat attttccggt aataccaact
    61021 ttttgtaaag cttcgtggat atctgaggta atattagcta gatttcgtgg ttggtcaatg
    61081 acatcactta aacctgaacc aaatggctca actaccacaa ccttatgagt tttacttaaa
    61141 ttactaatca aattatggta ctgatgtttg gcagtcaccg tcccttgacc aggaataaag
    61201 acaattggta ctttaccttc tccaacagta tagacattca tcttcttgcc tttgacttca
    61261 accttttgac catattcttt gataggtgtt ttgatatatt tttgatattg tttgttacta
    61321 ataaagctct ctgtgccttt attatatttt tcaagttctt tcgttaactt cattcgcttg
    61381 gctgctgttg ccttgtcttc cttcggattt gcagttacgt tttcttgctt aggatcctca
    61441 gttttagttt tttgattatc tttattggca gaacatccaa cgcaaagcat taaagtcagg
    61501 gcgatggttg ataatgtcgt tattttattc atcttttctc cttagtaaaa taattggggg
    61561 gaataattat ttttcatgcg attgaatcca ctcgtcaata gccttcgcca tatgtttata
    61621 ttgcgtatga taaatcaagt ggctagcatc tagtgtctca acagaaacgt cttttggatc
    61681 ttcagataag tcaatatgtt gttgttccca gcttttaagc ttagggaata tttttttacg
    61741 catttttata tcttctgctg actcattagc taagaaatac ttagctggca tacctttagg
    61801 gaacttaact ttttccattt tatgaagatt gtcactaaca cgcctatctt catcgcgctc
    61861 tgttttatta cctgattttt tagcaccgat tgataagtat tgttcattgg ttttcttatc
    61921 aacttctgga atggattctg cccattttaa ggttgaagct gaagattctc cagttgtgcc
    61981 atcaccttcc atatttggtg tgcttgtatc catgccaatg aaacctttga catcgttaga
    62041 atatgtctta gcataattta gaccgtatat gccacctaac gagtgagcca taagatagaa
    62101 atttttgaca ccgacttttt caagagctgt atgaatctct ttggtaatat tttcaatcgt
    62161 acgtggctta tctgtcatat cactaagccc ataaccgagc ggttcaaccg tataaatttt
    62221 atattttttc cctaatctat caagcaagtt tttgtaagca taacgtggtg aaatttcagc
    62281 ttgaccaggg aagactacta gcggaatttt cccttgaccc attgtatata tgttcatttt
    62341 tttaccgtca ccgacatcaa ttttttgtcc gtaatcttca attggtttcg ctttatattt
    62401 agttaggtca atagcacctt tagcaatcat agctttttct gcttctctct cttgtttttg
    62461 cttagcgatt gacttttctt gcttaggctg ctcagtttta gttttttgat tatctttatt
    62521 ggcagaacat ccaacgcaaa gcatcaaagc gagggcaacg gttgataatt tcgttatttt
    62581 attcattttt tctccttgac ttaggtattt cattcaacga ttacttttga tgggattgga
    62641 taaatccatc aatagcttta gccatatgtt catattgtgt ttggaaaata ttgtgactac
    62701 ctttaagaat ttcaacggaa gcatcttttg gatcttcaga taaatcaata tgctgctgat
    62761 cccaattttt caaaccttta aaatctgctt gacgtagctt gattccctcc acagattgat
    62821 cagcaagaaa atacttagtt ggcatccctt tagggaattt tacattcttc aatttaccaa
    62881 gattatcacc taagcgctca tcttcatctc gttgagactt attgccgtta atttttttcg
    62941 caattgacag atactgttcg ttagtcttat catcaacatc tggtaatttc attgcccatt
    63001 tagcactttc agggtcatac tttgtttttt gttccccttc catccaaggt gtactggtat
    63061 ccataccaat aaatcctctg acttcttctg gataattttt agcataattt aaagaataca
    63121 taccgccaag tgagtgagct gcaaggtaga aatttttaac tccaatttta tttaggcctg
    63181 tgtgaatttc ttttgtaatg ttttctagcg tacgtggttt agtaggaata tcactgagac
    63241 catagcctaa tggttctact gtataaattt tatatttttt acttaaccgc tcaattaaat
    63301 tcttataagc atagcgtggc gaaatctcag cttgaccagg aataaaaaca attggaattt
    63361 ttccctgacc agtttcataa atgttcattt tcttgccatc accaacatcg atttttttac
    63421 cataatcttt gaccggtttt gccttatatt ttgtcaaatc aacagcgcct tggggatgca
    63481 tctcttgatc agcagcttct ttgtttttgg gttcttcttt ttgcttatct gtgagtttag
    63541 tttgtttagg atgatgagac tgtgttttag atgtagacgt gtttgatgca gcacacgccg
    63601 tacaaagtag taggcttaaa gcaacagttg atagctttgt tatattcttc atgcattctc
    63661 cttattgtct ggaaaacata gctttaccaa agtgtcaaac gtttttctgg ttttagatac
    63721 attgaatcgc cttttttaac accaaaggca tcataaaatt cttggaaatt acgtactggg
    63781 atgttagctc tcaattcata tggtgcatga acatctgact gaattgagga cttactttgt
    63841 tctttggttg ctttttgacg ccaaatactt gcccatgatt caaaaaattc tttatagttt
    63901 ggattttttc agtcttaaga gcttctagag atgccataac accaccatta tctgcaatat
    63961 tttctgctaa agttaattta ccatcaactt taccgccatc tgctttaagg ccatcccatt
    64021 ggtcaatcat agcttgtgtt tttttcttat aatgttttaa atcttcttta gtccaccaat
    64081 cgtgaagatt ccctttctcg tcatatttca taccattaat atcaaatgag tgtgaaattt
    64141 catgaccaat aattgctccg atagctccat aattttgact aactgtttta gttttatcgt
    64201 agagtggtga ttgaaaaatc gctgctggaa agactatgga attggtatta ggatcattat
    64261 aagcatttac agcattggca ctcatttgcc aatgttcacg ttgattagac tggttaaatt
    64321 cttcaaatgt tttcttgttc gataattttc tgtagttatc attgttttca aagaagcttg
    64381 ctttactatc aaattggtac tgacgataaa gatcaggata atcttctgga tagccaatca
    64441 ttaatctcat gttatcaagt ttcttaatgg ccattgcttt tgtattttct gataaccaag
    64501 tgttgttttt taaccgtgtt ttatagacat taatgatttt cttagccata tgttcgacgt
    64561 cttttttagc tgcctcacca aaatatttct taccataata atcacccaaa atatggccaa
    64621 aagtatattc taattgggtg taagcatttt ttctttcgat tcaatctgag tcaaaccaga
    64681 cgcaacattc ttaaaagcac tcgctgcttg acgattttgt tctcctagaa aatctgattg
    64741 atcaactagc atagaaatca tcatccatgc tttcatattg gctaattgtt tactatttat
    64801 aacatcatta acctgtttaa aataagtttt atcttctaca ttgactgcct tattcgttgg
    64861 cccaactagt ttttcaatca attctttaaa gtcaaattcc ttggcgtaat tgtgaaccgt
    64921 ttccatactt tcagggttat atcgccccgc agctgtctca ctttcagctg tgattttact
    64981 ttgatcaact tgcgtttttt ctgataagag tctatcaaat gctatagctt gtttaactag
    65041 ttttctatct tcaatgttac tttttccagc ttgtttaagc aaagccattg ctgaagtacg
    65101 gtaagctgat aatttagcct caccttcttt attgcccttc ttatattggt caggtgattc
    65161 aagtaatgcg ggtgcttgac gaagcaccaa ttgcttttgg ctattatctc gagcattggt
    65221 ttccacagtc aaaccaaatg gtaaaacaaa accactcatc acaaaatcat gggccaaact
    65281 ttgaaagtct ttcattgaag agactgcttc aagtttttgt aaaactggtt ttagaggttt
    65341 gagaccattt ttatctcttg ttttaaagtc cataccttgt ttatagtatg caaccatttt
    65401 tttctgttca tcattggttg tttcaatctt tcctgaggcc attttagcca tatcagacac
    65461 taacagttgc tttaatttat cctcaatttc tgaaaaacta ttaacactag tctgcccttg
    65521 tttgagtttt gtattggcta gccaattatc attgacagcc ctgtagagat tttctctcgg
    65581 actgacatta tttgctttct tctcactggc agatactgca tgtaacatag caatatcttt
    65641 aaccacatat cctgatataa gacaagctgt taaagtaaga acactcatgg attttaaata
    65701 ggattttctt tgcataaatg acacctctct ttcttattca cttttagtat agcaagtagg
    65761 gtgttaaatt cttgttaaga attcactttt ttacacttat acaaaaaagt agtagctaat
    65821 gagctactac ttttgattta agagataaaa ccttttatcc atcacaatta atgttgctaa
    65881 atagatttta ttttatcatt aatatttgct tttaaacttt ttagctaaac taaataatac
    65941 actcagcata acgatagcca ttccagtgat ataataacca cgtttgctgt caccagtgga
    66001 tggcaatgta tttttaattt gtttttcaac atgtgtctga tgtgaagtta tctgtttatt
    66061 gtctgttcta gccatatgaa ctgcggaaac tgtcttagtc tttggtaaat cagacgcttt
    66121 aactgtagta tcagtttgat gaacgttatt aactttgtag ccttcttcaa ctataggtgc
    66181 ttcttgggtt ttatgtccct cttcagcttt acgtgcttct tcgactttat gtgcttcttc
    66241 agctttacgt acttcttcag ctttatgtgc ttcttcagct ttacgtgctt cttcagcttt
    66301 acgtgcttcg tcagctttat gtgcttcttc agctttacga atagcttcta agtcaactac
    66361 tttaatcgga acatcaactt tatcaacacc accatttaca taatgaatcg atactttagc
    66421 gtttgttaac attcctgatt catttgtgtt tggagcttcg tcgattgtta atgtataagc
    66481 tcctgccatt tctggaacaa ctaagtgctt catcaacata tccacagtta ctttagctcc
    66541 tttgtcagct gtcatttggc ttgctttaac atcaaaacgg tttctataga tatgaagggt
    66601 ctcattacga cgataatttg ataagtcaac agcagataac ttaataatat tatctccaaa
    66661 ttctagtggt actgtcatat ctttagtgat ttctacacca tttctaaaac tataaagacc
    66721 ggttttatca acagattcaa gtaatgaatc atcttgatat aattttagtt ttagagagtc
    66781 atctttggca acgatgtgga taagagcagt atcagaagtc aaatcacgtt tagcaactgc
    66841 tgtgttaagc tctggaactg attggtcaaa gaacatgcga cgaacaatac ttgctttctc
    66901 tcctgcttct gttaccactt caaccctaat ccaagtgtta aaacgactaa gtggtaattt
    66961 agcccggaac tcatattgtt taccggcata aagtactcta cgtccactca ttaatggttt
    67021 tgttcgatta gtcaagtctt taccttgaat aataacaggg gttcctagca tgtctccatt
    67081 ttcattcacc attttgactt caacatgctt gcttttatca ccatccttga tgtgaccgat
    67141 aataccagat gctgtgattt ccttaccatt tagagttgcc tcactagcag tttccaataa
    67201 ggcaggagtt aagagaaata ctgatgggta tttatgttca ctaacaggat catcttgtgg
    67261 tccagcaact gcaaactgtc tcatcatcat attttcagta agagcactcg ctcctttaac
    67321 agcattaaaa ttaagtttag tatctgcagt gagattaatt cgaaggacta gactgtcact
    67381 tgtttcgtaa tacgctgaat ctttttgcat agtaagtagc atatccctca tatgcttatt
    67441 tttagataat ctaggactta aaggaagttg ttgaactttc ttacttacac tacccgtctc
    67501 cataatcaaa cttttaacat ttaatgaatt ttctgtttta tgtaatacaa atgttgtcca
    67561 tcctttagtt aaaggtaact cactaccttt accaacttct tttccattca cagtaacctt
    67621 actataatgt gaccagtcaa tattagcaaa ggttactttt gctgtatttt cattggcttg
    67681 agttgaatga ctgagctgtt cacctttttt agtcatatct tctttctgat gactatatgg
    67741 atcttcttgc cctttataaa caactttaag acttgcacca ctggttaatt taaatttaag
    67801 gttaattgca tctttataaa tatagtatcc atctatctga ctttcagtta aaccaaaact
    67861 agagtcaaat tttttcaatt catttggaac accattttgg ataaggtata tttttttaat
    67921 aattgaagct gggtcttgag attgtttgaa aaacatatct aattcaactg ttccagtttt
    67981 tgcaataagt tgtcccttaa attcagaatc ctttcgtcca agtcgaatag ttttaaactt
    68041 actcatgtcc atatcgttta tatccaaaga aacatctttt tgttcaacca attctggcat
    68101 ctttccatct ttgaaagtgg tcatgatttc tgaacttgag ttaatgttat taatagtaaa
    68161 ctctagacga cgatttgtcg ggttgtattt taattggtaa gttttgctga gattaatgtc
    68221 ttgttcatca tttttcaaga tattatcaac agttttaacc tcattcaaac gacgaacaga
    68281 taaactatca atatgtctga catcatttgg taattcaata ccaatcttaa cagatttatc
    68341 tttaagcgct atacgagaag tttcttgcaa tttatcgtta acataaatct taccaccctt
    68401 gtctttaata gcagcaaagc ttgaaaatac aatggcatgg taatctactg tttcaccagt
    68461 agctaattgc tcaaaagtag tattgctcaa atagtcaacc gctgtgatat tgacatattt
    68521 tgtatctgca aaataatctg ttgtgatagt aatttcttta ttgcgattat tattacgata
    68581 aattttaaga ctagtgtcat caaattgatt agctgacaaa gcatttctag gaatgactaa
    68641 tttgccatca gattcgactt gtaatatttt gccatttact ttgctagcta aaatagggtc
    68701 atcaacaaag ctaagttgaa gagacttagc atatttaatt gctcctgatt tatcttcaat
    68761 agcgtctttt ttactatcct ttggtgcttc atccggcggc gtgtttaaat ctgggaaacc
    68821 tggaggagtt gataaactat ctttgggttt gcttggctta gttcccggtg gggttgaata
    68881 atcaaaactt ctcattgaag ttggtgctgg taaatcaatt cctggtggac ggtctaattt
    68941 tggtttttca acttttttat cttctttttt aaatcctgga ggtgtgataa ggtctaaatt
    69001 attattgcca ccaccaacat acttaacaga ttgaagataa acgtctttca aaccaatgcc
    69061 agtatcctga actttgaaag taattttttt ccagcgatct tctaacttct ctacagtagt
    69121 cttatctttg ggacttaagt aaggtttatt gttgtccatt ttgatatagt attgataaat
    69181 ttggacacca tcgccaccga cacctttatt attcaactgt actttaattt gataaatgta
    69241 ttgtttatcc ccttttgcaa tttaatcttt aatcttacca tcccaaatag aatctggcat
    69301 catggcaaaa ctattacgat aatctaggcg ataaagtttt ctaacgtcgt gtgatctacc
    69361 aagtacacga acttctttat tggtatctgg atctataata gaatattgaa catactccat
    69421 attacgcaaa gctgcaatac gaactcctgc tttgtcatag aaaggactat taggagagaa
    69481 aaatactgta ttgttataga ttggaagact caacatgccg ttagttgtaa aggttgatga
    69541 ggtcttgttg gtttctttat taacatagaa ttgaacgcga cgttttttgt caccgttacc
    69601 aatttcagaa atgccttcaa aagcatcaat agctttttgc tctgtccatg aaccgtaaaa
    69661 acctaaaaat ggtaagtgta aatcggctac accttcagta tcttttaagt taagataacc
    69721 ttccaagaaa ttattgcgag ttaaagcctc tgcatcagaa taatccacat caaggtcaac
    69781 agctaatgtc ttacctgctt ctacagtaac tttccttgta actgcatcag ctttttttga
    69841 tactaggtgt tctgaatttt gcgtacgaaa tcctttttca tcaactgggt ccgcaatcgc
    69901 ttctgatgag atgatatacg ttttattagt ttttccaaag ttacgaagta gaatgcgtgc
    69961 tttaaatttt ttctctttta attcacgtaa ctccaactta ccatctgcat tattatcgtt
    70021 agtccctgtt acagttgcaa cgactgtcgt aacaagagct ttggcaagat tcattgctcc
    70081 gctaccttgt tggcgcacaa agtaaggtgt tttgccatct tttgattgtt tattgataat
    70141 tggctgtgca gtgttcatca agagtaattt aataaaatca gcagcattat caacaggaat
    70201 ttttttatct gtaatgtatt gttttaaaac tgcagctgcc ccagctacct gaggtgcagc
    70261 cattgatgtt cctgatttat cagcatattg attatcctcc acagttgaga aaatatgacc
    70321 acctggagct gtaatctcag gttttagacg taagtctggt gtaggtcccc aagaagaata
    70381 agaagataat tgtcctgcta aactattatc aatagcttga cgctctgtta aaatagtaat
    70441 aattttattg ctattatttt tgatattgtt atacgtgctt ctggtaatgc gagcaatatt
    70501 aaatttagct gcttctgatt caactaataa ttgactgcct aaatcatctt taaccttcgc
    70561 attattgtaa agcaagatag cagctggatt taacttagta atcgcctgta ctttttctaa
    70621 aaatccatta gatttattat cttctggaat ttcaagcatg gcgattttac cacttaaatc
    70681 aaaatgtttt gacaattccg aaggagcttc tgctcccaaa tctacagcca atccaataat
    70741 agtttttgaa gcgtttgggg caacatgaag attaatgact tctgtaactt ctgttccaga
    70801 ttttttatct ttaaatttca aaacatgagc atgcttttta aggttctcca tagaggcaac
    70861 agccaaggta ttatcatcga gggctgggtt ggcaatcaag gctgtatcat aattttctgc
    70921 tagcggctta acaccataag tattaccgtc agtgatattt ccttcattac ctgctgctac
    70981 tgcaatgacg attcctgcct tacacgctct tgcaataact tcaagttcag gatagtcttt
    71041 cccctcatgt acaaatccag caggtgttcc aagactcata ttaacaacat cagcttttag
    71101 taaaatagca tcgtctagag cttttagcca cacatctgtg aaggttgtgg gattttgttg
    71161 gtcatctgaa aataccttca tcgctaaaat ttgagcattt ggtgcaacac catacaattt
    71221 ttgattatca tcattagctc ctacgatacc tgttacatgc ataccatgca ttacaccgta
    71281 gctatccttg aggttatcat tatagtcata atagttataa ccataaggta ctttttcgga
    71341 atagaatttt cctttgagat tcttttcatg aatcagtttt tcaatactag ttttattata
    71401 cttagcacga tttggttctt tgatttttag tgcttggtgt ttgaaatcaa cccctgaatc
    71461 aataactgca acaacagttc cctcaccttt atactgggag ttgttccaaa cttctggcaa
    71521 accaataagt tgccctgagg aagatagtaa aggttttgtc tgaacatatt cttgtgagac
    71581 atagaccctt ctaacatcag caatcgtttt taatttttca atttcttgag cttgaatatc
    71641 aagtgcgaca ccattaataa tagcatcgta acgcttcact ttgcttgaat caaagtgaac
    71701 acttttttca atattcttaa gaactttatc ttgctcttgc ttgatgcttt gtaatttttt
    71761 ctcaacatca gcttgattta actgtgcaac tgtttttttc tgttctgtcg cataatctaa
    71821 gatagattcc ttattaaatt caacaattac tcttactttt tgaggctttg gcacagccgg
    71881 agtagctttc tcagcaaccg ccccctgtgg tttagtcaca gtaactgcct tagttgcttc
    71941 tgtactctta ttgcctttct ctgagttttc ttactcgtat ttaggcaact gctttggctg
    72001 attaacaact tctggtgtaa cagttacttt tggagctacc ttcttagtat cttgagctaa
    72061 gttaggaatc tcaactgttt tttcattaat tgttgggggt gtggttgtaa taggttggct
    72121 aacgtctgag ttaacactag cttttactgt ttctggcctt tctactacct tatctctagg
    72181 cattactcgg ctctcagatt gaacagtttc acttttgaca accttattct ccttagcttc
    72241 aacaggagtt gtagttgctt gtgtactgtg agtcttagag gtatttagag ctgttgtgtc
    72301 tgcttgaact ggctgaggta ctttcgttac agtggtaggt gaggttgttg cttcatcagc
    72361 gtgaatctct gtacttgctg tagctagact aacaactgct actgtaccca ataaagcacc
    72421 gcataggctg cggcacactt tacttttcct aaatccaaat ttgtccgtct ttttataaaa
    72481 tgtcatggct tcctcgtttc taaatttttg tctacatctt taatcatcat taagaaataa
    72541 tccatgctga atactcggca acggatcaac catatcatca ccattgtcat aaacaatatg
    72601 attatctagc cctaaattgg ttatattatc aacggtacta tttttcaaaa caacagttac
    72661 ataccgacat atgtctgaaa attctaaata atgaagacgt gaatccataa aaaagacttt
    72721 gataggctct ttttgaggag gaatttcgac aacaactttt ccatcatagt gatgaatata
    72781 tttcgtattc tctataattc ttaaaatagt attagatgaa tcattatgac caataggttt
    72841 ggctgaaagg aattcactca taaaagaacc tcacattact taaccggttt gttaaccagc
    72901 taacgaaatt ttagcactcc tctaagtact tgtcaatggc aaatgttaag ttaaaatcgt
    72961 tttcagcaat aagttctttt atcttttttt acattagaaa atatttcagt ttttattatt
    73021 tctaattatt atcacaaaaa aacacctcta ttttctgatt ttctcataaa atgtggaaat
    73081 gatcagttta tcatatcaac tctaaccata attgttaaag actacccaag gtattataaa
    73141 aagcccttga aatacttcaa agtgcttttt atagatgcat atgtaggaac aacatgttgc
    73201 gagttagtta tctggtaata attcttttaa gtaaaccgct tctactttaa aagtattatc
    73261 aaaagcataa ccataaatat agccatctat cattgtttga taggtaatat ctatagattt
    73321 cccacattta taggaaattt ctttttgtaa ccatgcctta taaaataata atttagaatc
    73381 catatcacta ctaactgatt ctcctaggta gttttggtag gtttgttctg atctatcttt
    73441 gtatttctca atatcaaccc cagttgaacc actgggagca taggttagaa tatgatagtc
    73501 accagaatta gagtaactaa ttttcccctt tggcaaacca attaaataac tactaacact
    73561 agaaaaagat tcctgacaaa tcttattggt aaaacctaac ttttttaagc attggtacac
    73621 aaaataactc cctgttaaca gtttatcttt gcttacctgt ctatttgctt gttgatatct
    73681 catcaaccga tcgccagata aattattctc caatgtagac tgcctatttt tataaatcat
    73741 aatatagtta actttgtctt tttctgataa aggattcaat ttctgcctcc gctttttgta
    73801 aagatgcctc tgcttcttct ttaattcctt ggcttagtct tatcatataa cttttataga
    73861 tatcatagtc tctttcaaga cagctaattg ctttacgtat ctctcttaca tcaggtttct
    73921 ttaccaaata agcgcacttt tctttactga acttcttaat attccaaaat tgttcgttaa
    73981 agaaagggat acctataaag ggaatacctt gtgcagccat cgcataaact gttccttgcc
    74041 caccgtgtat aatagcgaaa taaatctctt tatctatggt tttaaaatca ataaaatcaa
    74101 ctatctcaat ataacttcct ggaatgttta tagcctcacc acagcttgtc agtaaataat
    74161 gataatgcct tggtaatgtc tttaaaaaag attccaccca atctcgttta aatgaagtac
    74221 caaagctgag cagtacagtc ttattgtctt gattaatttg gctgcatttt gaaaaaccta
    74281 cattatctat caaaataggt cctatcgttt tataggacga atctttatca aattcataaa
    74341 acaaagagta atctgttacc aagtttagat caccagtaaa taattctaaa gcattattaa
    74401 aaggtttgcc accatatttt ttaatatagt tattccacgt tttaacattt gttgaataag
    74461 tagtgattac tcttttgata aaatcctttg tttgaggctt actagcttta cttatcttcc
    74521 cattaggcaa tattccttct gagttttcaa caatttcact gatatgcgtt gcccctgaaa
    74581 taatccaaat taatggtatg ctttctaaac gacaacttgt aacaatagat agacggtaac
    74641 ctgtcaatac agcatcaaat ttttcttggc gtaacaagtc tctttcaata gaacagatgc
    74701 gttcaatttc ttcttctgat aaaaaattaa agtctgttga aagagaaaag aaagcatcat
    74761 atcgcttact attataaaca gatttatctt ctacacagtg gtgatagcga taagcatcat
    74821 tattaaataa aaatggataa taattgtctc caaggatatg aatatcatgt ccttgttcat
    74881 ataaacaatt agccagtttt gataatcttt ggctctctcc taaattcatc atgaaaggag
    74941 caaataaaaa tttactcatt ttatcacctt ttttcaataa tagcaaaaga gataccaccg
    75001 aagatcgaat aagataggac taaataatag cccttttcta tactttcatt aacagtatag
    75061 tccagttctt caccagcacc attagatgaa aatccaaact gaccagaagc aaggtttggc
    75121 atattataat actcagacaa gttcgctaag aaatcataat ctgaactaac tgccttcttc
    75181 cgctcattcc aaacgaaacc tttgatatct tttatggtta gtcctaagtc tgataataaa
    75241 ttttgaagcg cagcatcaaa aatagtcatc acatctgtga atgttttatg gctatatttt
    75301 aattgtttac tacctaatat tataggagaa ttatccaatg cttgacgaga gaggacttgt
    75361 gctgaacaat aatcagaacc gacaaacatt tgactatcat agtttaattg ttgccaccac
    75421 ataaaactca tgtctgtcca ctgattagca gaaacaagaa tcacatagtc tagattatcg
    75481 ttacgcatca tttccttggc atattgtata ccatcaagcg ctccactatt tgtcgaaatg
    75541 acagataaag gacctgttat tttaaaaatg atagaaagca taccagctgc tgcattcatt
    75601 actgtaaacg ggaatcgtga agcagaaaca tgtgcatatc cttctgttgt gatttgcttt
    75661 tcaatacctt caacaacctc aactggtcca gaaagtgttg taaatacaat tcctactttt
    75721 gaagtatctt gtttttttag attaatattg ctttctatta gtgcttgagc tgttgttacg
    75781 gcaaccattt tggaaaaatc atccattttc ctaaattgtg ctgggttgac agttttgggt
    75841 ggtctagccc ctttaaagcg taatgcttca aagtcgttga aattactagc aactttttca
    75901 taggttatag aaagtgattc attcttagaa atggaagcaa cagatgataa gatagccatt
    75961 ttaagatttt ctctagcagg taatgtttct agaggtgaat ctaaagatga caataagaca
    76021 ccactattat ttccaccaaa agcaaacgaa aaatttaaag catttcttat tgggtattct
    76081 ctcttttgat gatagacaaa attttctgga aaaccttcta tcccaatctc atttttagtt
    76141 gctggtacag tctgttcctc tattgccgct aaacaattaa tcaattcgat aatacctgca
    76201 gcccctagag tatgacccgt ttgccccttg gtactgctga tcaatgtcgt tgtcgggaaa
    76261 aacttaccat acatattttt ttccatttta tcattagctt gagtacctgt accgtgaccg
    76321 ttaatatagt caatctcact gtagtcaata cctgcttgag tcactagctg ctttgcaatc
    76381 tgtgccgccc cttcacctgt tggcttaggt gctgttatat gataaccatc tgaagtaata
    76441 agaccaccga taatttttcc atatttagct aaggactgat ctttgacaag aacaacaaaa
    76501 ccagcgccct cacccaaatt gattcctttt ccagaagaat agggctgaca tgccatttct
    76561 gtattaatag ctcctagtga tgtgaagcct gctaaagaaa tatcacttaa ctcatcacag
    76621 ccaccacaaa tagctaaatc acaatcgcca tcttgaagta attgtgttcc taatattacg
    76681 gcattattac ttgcagaaca ggcggttgaa ataacatacg aagctcccac aatatcatga
    76741 taagccatca attcatcagc aatatggtaa acagatgctt tttctaataa actagcatct
    76801 acttgacgct ctccttcttc aaattgatac aaggcatttt gaccagcact ctttccccca
    76861 agtgaggtcc ctaaacacac agcaatatta tgataagctt ttaaattaac acctgaagaa
    76921 gcaagagcct cttcaaaagc ggtaaaagca aatttaaaat tacgtgtctc atctttgtat
    76981 tgctcaggaa cctctgggtc actagttatg cttcctgtat aagattctaa aatagagtcg
    77041 tgatttttat ataaatgttt agaaattcct tcttttaagt cgaagagatg ctgtttatgc
    77101 tcgctataat tctttcccaa agaagaaata attcctattc cactaacata tacgctcatt
    77161 ctttgttcca actttctggg atacaataag ggcttgattg tgttttcaaa atttggccat
    77221 cttctgttac aaaagtaact cctgaatgag catatgtttg gttaacaatc atcaaaccta
    77281 ggtaattttc cgaagagtct ttctcgtctg ctatccaaaa aatctttcca acaatttggt
    77341 catcaaatag cactggtgtt cctgaagcga gtttttcctt cgttgagaat ccaacacttt
    77401 taactgttgc tgatctgatg tgctctaaca agctatcgcg acctctaaat tcttcctttg
    77461 tgaaatctag attccaagaa taacctattt catataaaga atattcttct tgttgataaa
    77521 tatcagtaat gggttgtccc acttcaaacc ttaaatatct atcaagttca tccccatact
    77581 ctgctatacc ttcacagaca tccgaaacaa aagtagcaaa aatagaagat ggtaggaaaa
    77641 attgatagcc aaattctcca gaaaaaccaa atctagcaag ataacctttc tcacctttat
    77701 aagtcatctc tatcaaagta cgaaaattaa gtgttgaaat atcatattca taaaatgact
    77761 gagcaatttc tcccgaatat cttccttcaa tttgcagcat tttatactcg tcagaaatat
    77821 ctgttacggt atagtcaaaa ttgatgttgt ctaaataaga atccaaagct ttatgactag
    77881 ccaaccaata tttatcatca aatttgtaga aagtcacatc atcataaact gttccatctt
    77941 cattgagtaa aaaagtgtag ccacaagtat ctgtatctgc aaatacaata ttcttaggaa
    78001 tcacaaggtc taaggcttct tcacattctt caccagtaat ttcatataaa tacccctcat
    78061 aaactttata acctttatct gttctcaatg tattataaat agtattcatt tttcttcaca
    78121 ctttctatat tctgtaatgc tcacatttgt agcataaggt gagttccttt taaacatgca
    78181 ccttattttt aaatgattgt tctctttgta agaataaaat attccatttt tagcagaaat
    78241 tagtatatat ttatcatcgc tagctacata caatactaat tgttgactat tttccaaaac
    78301 ataggattta ttatcatatt tgacatgttc cccttggcgg atttggacaa actttcctct
    78361 aattcgataa tcaaaagaaa tagaatataa ttcactgtct cttggaattt cattcttagg
    78421 caaaacaatc tgattatagg ctaaaaccag tttgtgacta ggataatgct ttctgttagc
    78481 tatcattctc ttgatgccat aatccttctc ttcttctaat gttaggttag agatatgttt
    78541 taaaaggtca gttgctgacg cttggtagtt gctgtcccat attggctgtt tttttagatt
    78601 gattaacgcc tcctccgatg atgcttgtga aatcctaaaa tgataagtca gtggaaaaga
    78661 aaggctatct gtatcaataa gacataaggg tctaaattgc atttgattgc tggctttgcc
    78721 aggaggagaa taggaaatca ttttatcttc attcgggagg taataatgga attgtctatc
    78781 ttcaaactta tctgctcgaa gaaaatcatc atgctcaatc gggtaacttc ctggaacatt
    78841 cacaagataa ccttcactat ctctaatcaa ctgctcttgg cagtgtaact tgaaagtctg
    78901 tttcaataat tttgggaaca tctctttagc cttttgatta catttataaa cacaagtgat
    78961 ttcttcgtca cttgtaaatg tcatgatttt ctcaattaaa gcaccttgat agaaacattt
    79021 gattgtaatg tgattttctt cttcttttac aactaatttc ttgtccaaag gttggctaaa
    79081 aatcaaaaaa ctatcttcaa gtatctttct tttctgcttt tcaaaaataa ggctgccatt
    79141 tggagagata agtaaatcat aaccttgaaa agttctctta agacaaatag ttgcatctct
    79201 ataatgcttt tttcgacgtt ccagttgaac atcgatatta ccataaaggg tttccacagt
    79261 tgcttgaatg taataataat tggcaaaacg acttaaagtt aggctagggt gagccctcgt
    79321 taagtttgcc aagtgatatt ttccagtttt aacccgaacc ctctttactt gttccgataa
    79381 aaagtcaact tgtaaaacat aacggttatc taacttagca atttccattt gaaacaagct
    79441 catttttaaa taataaggaa gtgaagcact atcttctgct ttaaataata tttcttcatc
    79501 agagactttg gtcctaattt ctgtctcctc ttccaaggga ttttcaattt cctccataat
    79561 ttgaaaagtt gatatgtcgt atgttttacc ataataattc gaaatacgaa acgtattcaa
    79621 tatcttaggg aggtgactac gcaatgatcg acaatgcagt atgtcttcat atgtcttatc
    79681 atactctttg aagccattca attttgataa agctaaggag ggtttattgt agttactgat
    79741 ttcggtatag atattagaaa acttcagttt tgttatcttc tttaagatag ttttatatag
    79801 ataagtgatt gcctgtccac tacgactttt cgaatcaatt aaaagaaaac cactaaagct
    79861 actattccaa tctaaacaac cataggtaat aaatttaaag aaagctgatg ttccaatcaa
    79921 aacaccatga tttttcagta aaaataaatg tgtcacttct ttaactttta acgttaaatc
    79981 aaggtcatca acagttaaag gcctaccgtc tggaaattga taaacatgat tcctattaaa
    80041 taaatgaacc acttcagaat aatcttctaa ggaagcttca gaaatcttta atttattatc
    80101 atctttcatt ttttaacctt tttaataata aaatgaattg ccaataaaat aagaatgagg
    80161 gcaatgatat aaatcaacat aaagtgatta ctcagccttt ctccatcagc attacgcaaa
    80221 acaacaccat atgattcttt atagttagag acagtggctt cgttggcttt gtcaaatacc
    80281 ttcgcaatag aacccttcat taagacctgt ttcaaaagag aattgatttg agtcaatgga
    80341 aaccatgtca taacctgttg taatgctttt cctaccgagc caattgagag atatactcct
    80401 gaaataaaac caataaaagt ccctacaatt gtgctaagag tcgtaaaagc tgaactagta
    80461 tgaataaagg ctaaaatagg taaaatcatc gccgcagata aaacagtccc taaggaaacg
    80521 atccctaaaa tagttaaata atccatagct gaatactcta gcaaggaatt aaagccgtta
    80581 aaaataccga tagcaaagat gcaagagaac atcgtcatga caataccaaa taaaacggca
    80641 aatatagcat aggacaattc aattttgata tttgatatag gactgacctt aaaatcttca
    80701 tttaattttt tttctttgtc ggacaccata actccaaagg cacctaatgt actagtcatt
    80761 gaaataatag tcgtcaaacc tgcaatgagc caataattaa ccatgtgaat agtatcactg
    80821 gatactgtat tactcctcaa tgctgctttt atagcatcta attgaatttt tcccaagaaa
    80881 atctgataga ccaaaattaa gataataact gataaaaaag acatgaaaaa cgctaaccga
    80941 tctcgcgtgt aaactttacg atttctagaa attagttcaa acattgtttt tcctttcttt
    81001 ttctctaagc aagttgagat acgccgcctc taagttcgaa tattcaacat taaatgtctc
    81061 aataatagat ttatcttgat tgtctgagat aacctgcatc atttcctcaa cagaaatatc
    81121 cttaaatact atctcccttt ctgaaaggac ttgacattta ttaacaaaat cagctatcga
    81181 aagttggtct aatgactttt ctggtttcaa taccacattc aagtttgttg tggaatgttg
    81241 ctcaataaag cttttaatat ctcccgaata ataaatgttt ccttcaataa gaacatttaa
    81301 aacatcacaa gcagacattt cttcaagata atgagtaatt agtacaactg tcatctgcga
    81361 ttgttgattt agttgtgcga tagcatccca taaatcgcga cgagattgag ggtctaaacc
    81421 agtcgttggt tcatctaata ataatagaga aggttgtggc agcaaggcgc gtgcaatatc
    81481 aacttttcgt ttttgacctc ctgataaact gccatatttt tgttttttta ttgctgtgat
    81541 atctagatac gtttgaagat ctttaaggcg attacgtact tgtgatttag ataaaccgta
    81601 caaagcacct ctagaaatta gattttcctc aaccgttaaa ttatcatcta agcgattact
    81661 ttggaagaca ataccaatgt gacgataaaa atctttaata gatagaggtt ttccatccac
    81721 ctcccactta atatcgcctg acgtttgctt aaaattttga attaaaaggt taaataaagt
    81781 tgttttacca gctccattag gtccaagaag gccataaaat tttccctctt caaagatgag
    81841 attaatgtct ctcaatgctt caaaagaacc atattttttt ccaatatttt tgagtttaat
    81901 ttccattttc tgataccaac atccttcctg aggcaacctc ttttttattc tcatctctaa
    81961 taacgacttg aatttctaaa agatgcccca attttgattg agatttacat gtaatgttta
    82021 gctggtctcc tggagtaaca attttcttga atttgaaatt cttcacactg gctaaataac
    82081 ctactttttc tgctattttc tctgctgtta attcatcaag agaggggagg ctaccatcaa
    82141 gtgcatctaa aatataaagt aaagctgcag attgagctgc catctcaatc atcaaaacac
    82201 ccggaactat aggacgttgt ggaaaatgtc cttgaaagta aggttcgttg atagtaatat
    82261 tcttacgaca ttcaatgata ccttttttat aactctgcac tctatccacc attagaaacg
    82321 gatagcgatg aggtaacatt tttataattt cttcactact tagcttggtc atctatatcc
    82381 tcactatttt caatcacaaa atctgctagt ctatttactg atgataatat tgaaatatca
    82441 tcatcattta gttcaacacc aaacgttgtt gaaatcccta ttgacagttc tagggcatca
    82501 atagagtcta gctctaatcc acgaccaaat aatggttgat cattcataat cagatcttta
    82561 tcaatatcca acattaaaga gtcaatcaaa atacctttaa tttcatcaac tacttcttga
    82621 cgtgttttta ttattttacg ttcttcagcc cagttaatca ttaatcattc ctccatcaat
    82681 cacaatattt tttcctgtaa tatagtttgc cttctcactt gctaaaaagc ttacgagcca
    82741 agccacctct tcaggttgtc caaacctttt tagaggaata agatgctctt ttagctcttc
    82801 tttattttgc aattcatttg tcatatctgt ttcaatgaat cctggactta cacaattaat
    82861 cgtaatacca tctgatgcaa attctttcgc tagagtttta gtaatggaaa tgatagcgcc
    82921 tttcgttgct gaatagttcg cttgccctat ctgccctgct ataccactag ttgaagaaat
    82981 attgataacc tttccctgta tacgcttagc cttcatgatt tttaacacgg ctttactcat
    83041 gttaactagc cccataatat ttatgttaat gacatccatc catttttcct tagacatcat
    83101 caagaaaaag ccatctctaa caataccagc attattaact aaacaatcaa taccgccata
    83161 ttctctaaaa attttagtca ccaatttttg tactgctttt gcatctgaaa tattgcattt
    83221 ataaacgtct aattttggta actcctcttg taaagctctt gccttagcat cattgctatt
    83281 ataaatagca atgactttgt aaccttcttt gtataactct tttgtaatag cccttccaat
    83341 tcctctggta ccaccagtaa ctattgctac tttcataact gtttcctccc cctctttttc
    83401 attttttgca actgactaga aataaaccta ttaggagaaa tatcaataat ctccgcatcc
    83461 ttttctatga tagtttcctt aatcgcacgc cagttaaaga gattggtcca ctgatataaa
    83521 atttcatcta ccaattgatc acaatgaaaa atatagtcat agtcatcatt ttgcgaaata
    83581 tctaggcttt caatcaattg tttatactgc tctttagatt catttaaaaa atcagtatga
    83641 tatggtgctt ttacgcctaa ctgctttaat ttataaaaat aacctttgtt agtcatcacg
    83701 tcatcaaaga aagaacggtt gacttgatcc atagcaatta caccagctct atcatcagta
    83761 ataatagcta attttacttt ctcagactgt tcttttaaaa catccttact aaacatatca
    83821 atgttttcaa ccatcaaaag ccaaagctct tcagacacat gacaattttc tataagttgg
    83881 gcacgacgtt taataaagtg aataatgttt tcaaaactag tactaccact agcatataac
    83941 acattgaaaa taccggcgct atgagctgaa aatcctatcc cagatactat tttttcatcg
    84001 tttgttagcc aatcattata taaaaaattt gctaacatag acgcatgaac attgtctaaa
    84061 cttaccttgt ggttaatgac atccatcatt aactgatatg attctaaatc agttgcttgt
    84121 aactttgaaa aaagaatttc accttttgga gttttaacta gttgacaaag gtgttttgac
    84181 tgaacattac cctgtcctcc atatattatt actgttttca ttattcaatc ctttccatta
    84241 atagtattgg catgttataa tcaacgacac tgccatcagc acagtacttc ttgattactt
    84301 tgatatcccc ataactttta tattcataag aaattcctaa ggttataact tccaacaagg
    84361 tttctccctt atgatataca ccaccttccg taagaaactc agaataccta atatttccaa
    84421 ccagttggac tactaatagt aacttgtctt taagccgcgt tttatacaag ccacattgat
    84481 tatcagaaca ttgttcacac ctactcaaaa tattagaacg tcccatatta tcaccttcaa
    84541 ctaatcatct tcgctctgat atcagtactg ttggaaaata aaatatttct aaacatctac
    84601 ttcaaacact actatggaca agccgtgcat tgcctttctt atgttttctt cctttctgtg
    84661 tcgtctgaaa ctctttaaaa tgaggtcatg acttaagtgt tgaattcaca aaatcatgag
    84721 taaaacactg ctataaacat tagtttaaat ttaaaataaa cattgtaaaa attaattata
    84781 aattcacact ctaattaaaa tatcattatt atgttaaaat agtaacaaag tatttcatct
    84841 ttgtcaacac tttcataaat gacatctgct ttttgcttac cttttataga ccaaaaaacg
    84901 ctcttagaaa gcacatctaa gagcgttttt aactttattt tggtaaagat atggtaatta
    84961 ttgttctatg agggacatta ttctcaacac taatagtccc tcttcacctg atatactccc
    85021 cttatagtgg acagtaaaaa aacaaaaatt cactagacac tataagggga gtatatcatc
    85081 actaacaaag ctaagttagt aagcctaaaa cttctaaaca ctcaaaaaaa ttaatggctt
    85141 gccttactaa tcaagaaccc gcactctcat atacttctac tcctttatgc caaagtatta
    85201 gtgataccat aaaagaaatc aaggaaatca agataacccc accaatatta aaatagacat
    85261 ttctatcttg taagaagtaa gcagcgggat aataagctgt aaaagcaaat ggaatgacaa
    85321 aactaatgat ccaacgcaat agattattat atattgcgac aggatactta gcaaagtcat
    85381 taaacatata aaaaatatag ataacagcac cagattgttt ggtccaaaaa gcgatacttg
    85441 acgtagcaat cttcaaggag gtataaatca aagttgcaaa ggggataata aatataaata
    85501 ataatacttt tggcacgatc cagcttatac tagatactgt ggtagataac agaataaaac
    85561 caaccaataa ctcccccaat gcatctactt gaaaggtttc aactaggaca tgaaataaag
    85621 gactgatcgg cctggttaaa tacttatcaa attctccttt tcgaataagc ctttgaccaa
    85681 gagcccacaa gttatcaaag aaaaggtgat caattccttt tgggaggaga gaaaaaccat
    85741 aaataaaagc tatttgctga aaagtccatc cttctaaaga agggatacgt tgaaaaagaa
    85801 cattcagaaa taaaagattt agcccttgtg ttaaaaatac tcctaaaacg cctacaaaaa
    85861 agtctatttt atactccatg atttgcttca tgtattgttt aataaaaata aagtgcattc
    85921 gctgatattt tgtcatctca acctccttgg atagtgatat gcgactgtac ttttttccat
    85981 attaattgag ataaagctac cattactatt agccaaaata tttgtaaaag taaggcttgc
    86041 acaatttgac tgccatcata tttaccaata ataatcatta ctggtgtgta aattaaagat
    86101 gaaaatggta aaaatcctaa aatatcagca acgattttcg gaaaaaaagt aagaggaatc
    86161 aagctccccg acataaatgc gactaacgaa ttttttaaca ggtttgaccc ccataaattt
    86221 ttaaaaacaa atgccgagaa gccaaaacaa atattaaaaa agaagttaat caaaaaagct
    86281 aaaataatgc taataatata aaataccact aacactatag cttgaatcaa atcagtcccc
    86341 aagaagaggc gtacccccgt aataacaagc aaaaacggta cccctatact taaaaagatg
    86401 agccatctag aaccaatctc catgaaaagg taggaagcag caaagtgaac tggacgtaag
    86461 agtcgcataa taattgaacc gtcttttact tcgtcaccta tcataaatga actatcagtt
    86521 cttgttaata aattagtcac aaaactcata ataatataga gaatcatatc tgacagctga
    86581 aaaccttgga ttaaggattg acttgaggaa tcgaaaactg ctttccatag ataaaatgca
    86641 acaaatgctc ctatcacatc acctatccta taaaggatga aatcaacacg atatgtgatt
    86701 aatccttgga tttctgtact aataaaaggt ttataacgtc tccaaattct gttcatcctt
    86761 atagctcatt tctatagaag cgacgaatga tatcttcaat atccgcatct gtcattttaa
    86821 ggtcacgtac agaaaaatct gctaatgtct gctgaattat gtcagcagtt tggtagcgag
    86881 aactatcata ctgaatatct aaaactaagt cattacgttt aatattaatc tccgactttc
    86941 caattaaact agagctgata tgctcttgtc ctgggcgcaa atcaaaagaa agagttttca
    87001 tctttccaaa tgtctctttt agctgagaaa ctgtgccatc aaatatttct tgtcctctat
    87061 caatcataaa aatacgatga caaagttgtt caatatcact gaggtcatgt gtcgttagta
    87121 aaatcgttgt ctcttcttct tgattaattt gtgtgattgc acgtcggata ttatctttca
    87181 ctgaaacgtc caagccaatg gtgggttcat ctaagaaaag aacctttggg ttatgtaata
    87241 aagaagctgc gatatccgct ctcatacgct gtccgagaga caaagttcta acaggatcct
    87301 ttataaagtc atttagttcc aaaacctcat ttaaaaaagc catgcgtttg cgaaattctt
    87361 tatcaggaac gtcatagatt tcttttaaca ctgtataggt ttcttgtaga gctaaatccc
    87421 accacaactg cgtacgttgc ccaaaaacga ctccaatatc cttaacataa ttttgtcggt
    87481 tttcttgtgg caatttgcca tcaatacggc aaaagcccga agttggtttt aaaatcccag
    87541 ttaacatttt aattgttgta gattttcctg caccgttcgc tccaataaat cccaaaattt
    87601 gtcctttagg cacatcaaat gttaaatctt taacagcttc aaaggtgtgt ttttcaggac
    87661 gaagaaatga ttgaaatgct ccttttaacc ctggtgcttt aacggttttt ataaaatttt
    87721 tctgaaggtg agaaacttca atcattgaca tattaatacc tcttttactc aacagtattt
    87781 ttatagctta aatcagctcg acagttagat tattaaacta taataatgat taacagctat
    87841 ttattatagc acaattaaaa attcaatgca aacgttttct tagatggtgt tactgcttaa
    87901 tattgaataa aaaagaaggc caacttagcc ttctttttta ttcatgtgat ttagagacgt
    87961 ttcttatacc ttcttttcct aaacgaatga cgtattaaaa gcattaaaaa gaaagaaaat
    88021 acaaagtaaa agactaagtg gaaaatctta ttaaggaaca taaatttttg ataatattca
    88081 aatgcttttg gtgccacgtg actagcatca ctatagactt tgttattcat aaaaagcacc
    88141 attgtatcat gcgtctttag atctgcataa ccaaaacttg aatactggaa aaaggcccca
    88201 ttagaattaa ctatacgacc atgcatgtaa agcccaccat aataatgtga tatcgcatca
    88261 ttttgatgtt tggtatattc atccgttggg aaaaagtacc ctttagaaaa agaccgcatc
    88321 aacacccaaa agttcagcgg agttgtgaaa aaatctccag ctccatatgc tgtggatact
    88381 tgattaaaga tattaatttt atttaacttg ccgttctttc gcattgggct aactaagtca
    88441 tgattaacaa ctgattggta actttgggta tcatctaatc gcaaaggatt ttttatcaca
    88501 tcatcaacaa gttcagcgta ggtccttccc gtaacttgag tggctatacc tgttagaaaa
    88561 ctaaagttga tattagaata ataccacgat ttcttattaa caaagttata gtgttttaag
    88621 caaaattgta attgttctct ctcatttttt agataaggag acaccttaac acctttttct
    88681 cttaacccac tcgtatgggt caacaattgg tggatggtaa tactattact accttttact
    88741 tggggaaaga actgagacaa tcggtcgtct tcactcaaga catcttgatc aattaatctt
    88801 tggataataa ttcctgtcat gagtttttgg agagaagcca ttggaaataa tttattattt
    88861 tcaatatccg tttcaacttc cccacggtta atcccatgcg agatggtaat cggtttacca
    88921 gtattatcgc taacaatcat cattccatta acattatttt ttcgcataaa gcgatgcaat
    88981 aaaatcggtg taatattccc tttattttct ggaataaaaa agaaacatgc cactgaaata
    89041 ccggtggcga ttactgctat aagaaagaga gataggagcg attttaaaaa tttacgtacc
    89101 ataaataacc tccttaatat cataacacag ggctaaatta tttcaaagac attttatata
    89161 ttctagcaaa aaatatgttc gtcgctaata ttattttcaa ctcctatcat tgctacctca
    89221 atatttcctt ttagattctc actatgatct tgatagttat aactcttctg ataagtacgg
    89281 attcctaaaa aaacttggta attctcttca gtcactttag ccttctcaaa attttctgtt
    89341 tcaagataag aagatatcgc ttctctataa aaaacattat gaaactctat caactcctcc
    89401 tgctttactt tcaagagaag caagctcacc atttctttac cctgtttaaa acactttctc
    89461 tagtgtcact ccaatctttt aaactgtttt taggacattt tttaaagaca gagataaggt
    89521 tatcaatgtc cttgaatcgc cttattcaaa caccaaactt gagcaaccaa taatctcatc
    89581 aaaatcatca agcacaaaca tttagcttta gaaactttga atattttaaa aagagaattc
    89641 atatcacttt gaatatcaaa aaggagaaaa ccaatttggt tttctccaga atataatttt
    89701 tcatcaaccc actacagttg acaaagagcc aaaaaacaag gaatcaatca agaaattttg
    89761 attgattcct cgttttatgc ttatatgaga tactttaaac aaattttttc aatgagatcc
    89821 aactatctag tgactcctcg atatcctttt atatctctca cgatagcttt gaggagtaag
    89881 tactattcca ttcgttttac ctataatttc accaaatgca tacataccat atcctatata
    89941 acggctatcg ttttaagaac aatttattta tgttggcaga aaataataat aggaagcaga
    90001 aaaatccaat aatattcaag agaaaaagta taggtgatag ctctctaaaa aaatttgata
    90061 ttgaaactat aagaaaccct gtagatacga tagttgaaac aacctttttc ttatccatat
    90121 taataaccac cccttaaaac aatttcatat cctatatact ttgtacgcat aacgaatccc
    90181 tcctaaatta tttttttgtc tgttgcaacc ctgacaagtt agtgtagcac gaaaaaacga
    90241 ttatattctc aaaatcttat cttctagctg ctcaacttct tcttcaatac gagggtcttc
    90301 atcattctta gtgttaaata aagcgagatt ggatcaacca gtgtcacgtt agcctctcca
    90361 agctatttat ttttctagta atcaaaggga ttggttcata attccaaatc tctagccgat
    90421 gactttgaag gatgtatagt tgaagacttg attcgaaact tttgtatctt ttccttgaaa
    90481 tagtatagtc tccgtatttt tccgaatcat caagcaagcc tagatatgat aagtctttaa
    90541 aataagtcgg ttaaaataat agctcttgtt ttaatttttg aaaatcctta tcacttataa
    90601 tcaacgcttc tctaactgga ctatctatta gatctttgga ctcttcaaat atcatatttt
    90661 ttggcttcgc aaattggtaa gatttctgtt tattatgaag ccatccacgc tctttaaaat
    90721 ttctaaagca acaatataag ttttagaata gaaagatcgg ttagctgact tacttcttac
    90781 atatcaatga ttaaaccttt agtatgtaat aataaggtaa tcaataactt ttgttcagtc
    90841 ggagacaacc gattatcaag ttcaattttt ttagttaatt tagaatatag aaaacctaat
    90901 tgtgggataa gagcattttc ttgatagtca aaataagact tcccaacatg cattaacttc
    90961 tccttctgct catcagatag cttattaaaa actagagtaa tgcccatttc aaactatttt
    91021 tagatagctt gagcttgttt gacaaagtta tctaaacgac ctgacctttt ttctttaacc
    91081 aaagggaggt ggaatcctaa cagttaagat tgaaaattca tatcgtcgac gaaaaacaat
    91141 tagaagttct agaactatta gctatatttg aatttcaaaa tggaaagcac ttaggatact
    91201 cttgattttt taaatatcca tatttagctc ctactatcat tatatttgca tcttatcaaa
    91261 cataatgata atatgcaaac ttttcgataa aatggacaaa tacgataata aatatttcat
    91321 ttgttagtat atcctcactt tacttggtca tataagcaaa attcatggta ctatctgtag
    91381 atgtaatgtg acgaaagata tagttccgta gcctatatac gaaccataac tactttccta
    91441 aaaagagaga atagtacctt cttttggaac tgtatcagtt tcaccatcta gtctttcggc
    91501 tgttccaacc caatcctgac catttcccat tgaactggta atggtaatct tctcaccatt
    91561 tcaacctttg agtttcagtc ccactgatta attcgtgccg caactcccca agtacatttg
    91621 ctataagcat aacccatgac atcatcaccg cccgaaatcg aatcattacg gacactttct
    91681 agtgcctttg gatcattctg ggcggttccc cttttggttg actaaagcta cgatcgactt
    91741 ggtagtacca ccccgttgcg ctggtttggt gttcaatgag cttgtcccca gaattcccct
    91801 cccaatggat gagaactcgt gaatcctgtt tttccatata tatttttcct ccgtaaacta
    91861 aaaaaagaga acactctaaa aatgttctcg taatgccaga aatttatctg acgtatctat
    91921 ttttttgtct acgcaagcca cttcaaaaca aacggtcacg tcaatatgtt ttttaatacc
    91981 ttttttatgt taaaatcact cttttcaata aagacaaaat gcttgaaaaa gtgcttaaaa
    92041 taaaggattt ttaccctttg cttcgttgta gcaacgctac gcactccaca tggctggtat
    92101 tatcagccat tgggtaatca gcctctttga agttagcttc atccacaatt gtcgtttttc
    92161 ctgaattttg aacgaccaca acataagtct taggatcaag tacgtaagca ttcggcgctt
    92221 tcgtttcttt aaggtcataa aatccaggtt taaggttgtc aaaggtaagg gttgtccgac
    92281 cgactgtcgt ctctttgctg acaggaatga aatctccttg tacttgatga tctgccgcta
    92341 ctgttgtgcg tttagacaag gtgaaggtcg cacctactaa gccttcttta gcttcgtcaa
    92401 tcttgttaat gctgaacttc ctgctctttt tatccgtaac cgtcaaaatc gattccggaa
    92461 tttgtgactt aatcgttgtc ccaatagctt gaatctgagt ggtgtagttt gcattaatag
    92521 gtggtgtaaa taaagtgctg gagttgttag accaagctac cttaatatta tctgctggaa
    92581 aaaaggcgcc aaaagaggat gatagaactt ttggatctaa atatctcgat ctatcactat
    92641 atggatcgcg cgccactaaa agataaggaa taactcctaa tggcttcatg atatcaaagc
    92701 tagactgact ttgaggtata aaagttttct cagatgagct ccgctattat aacgaccttt
    92761 atcagctcat actctttcat ttctaagaaa agcgaacaac tgaattcttt agcttcatag
    92821 atgataacgt aacaactggc aattctgctt ttaaaatggt ctttaggacc ttcatgaagt
    92881 acaaaactta catcacaaac gccctcagct atccctttcc aatgccaaat tagaagcgac
    92941 caacaagctt atcaaagaca tcaagcgaca agcctttggc tttcgcaact ttaaaaattt
    93001 taaaagcaaa attctcatcg ctttgaacat ccaaaaagag agagaaccaa gctgattctc
    93061 tctcgttgtt aatgataagt cacccattac agttgacaaa gagcccatat aaggtgtaca
    93121 ggtcagaagg gtaacataat ctttatcctc ttcaatgctc acggcatcca attggctagg
    93181 ttcaatcacc atgatacgat ccacttgata agccaaggtt tctttcagat tggtaacata
    93241 aaaataatca ccttttttca tcttatccaa atccgcaaac aaccgagcag ctggcatacc
    93301 tctttgaccg ctcaagaccg catgtgtaga agcccctcca atcggcaaac ttatcccttc
    93361 tagatgacca actccccatt gcagattgtc ctcttctgaa ccagcgtaga ttggtaaatc
    93421 ctgattgatt tttggaatca tcacatggtc aacctgctct ctgacttcta acatcctagc
    93481 gtattcagtc caccctgcct tttttgctcc tcagaataag aatctctcag cacagattga
    93541 gtcgactgac tacttgttcc agaaatacta gcattgtagg ctttagccag acctaaccgt
    93601 cgttgaatct ccgtacggtc aatggttgta actgttcttt taaaggcatt aatattggca
    93661 tgagaagctt gataataact aatctgactg acaataggga aaggccaaaa ctagtcattt
    93721 tctattattt aataatttca acgacacctt acaattgttt acgacggaaa gaacaaatgg
    93781 taagtatcat aaaagtagaa ccaactaata tatagacaat tgtaccaatt ccccctgttt
    93841 taggaaaaac acctggtggg cgtttgggag tgttggtaat aagatgttta ccatttcctt
    93901 caagataccc gatttgattt ttattagcat ttggatctgc tttcaggttc gtaacttctc
    93961 cattttgaat tgtaaatgtc acaacaggtt tcgttttaac ctctatatag ccatctggac
    94021 ttgaaatttc atataattta tagttaccat cttgaagtgc tttaaaataa attttaccat
    94081 cattctttgt tgtaacatca cttccctctg gaacaaattg cttataccca gaaaaatctt
    94141 tttctatctg aagttgaaac ttagctccca aaagcgattc tgaatgtttg tctttattaa
    94201 ctttaataaa ttcaacctca cccattttct tctgattact gatggttagt accggaaact
    94261 cacgaacatc acgaattttg ggaattggga aatcacgaat agtatttggt tctttttcac
    94321 tcttcggact tagcgttgta cgattatttg tattgtaaaa tttgttactt atatagttat
    94381 cttttaaacg tacatcatag gtaagaacta ctttttgtcc acttcctaag ttcaaatgat
    94441 tgattttgat ggtttgagat gtcttatcat aagtcactgt aacatctttt aaaattcccc
    94501 catcactgtt tggtccacca agagccacac cattttttaa ttgactgcca tcatttccaa
    94561 ccaaaacgta atcatcatgt gtaaaacttt gaccattttt taattggaat tcaatcatct
    94621 ctcccatagg atcagtcaca tttccatcaa caatagaatg tttttcctca acaattgttt
    94681 taaagtattt atttagctca tcataaattt tatttgtatc atcaacatta gtataatttt
    94741 ctgttttact tgatattgat tgcataaatt tctcagcttc aagaggagtt gcaccaggat
    94801 ctccgtttac accaatccca acagtaaaaa tgtcataacc tttaggtctt atatttccat
    94861 taaagtataa tgttgttggc tcaccatgag ttttgatttg tttcgttgca gaaactttct
    94921 ttgtcttagg atcaaatgga tagacccagt tgtaatctct ccaatagag
//
LOCUS       DATRME010000002        81249 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRME010000002 DATRME010000000
VERSION     DATRME010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 81249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81249
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..293)
                     /locus_tag="VBC23_000090"
     CDS             complement(<1..293)
                     /locus_tag="VBC23_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6842075.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILELIVQTIGRPFFTELSTFSTKEELI"
     gene            complement(317..1414)
                     /locus_tag="VBC23_000091"
     CDS             complement(317..1414)
                     /locus_tag="VBC23_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842076.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1417..2133)
                     /locus_tag="VBC23_000092"
     CDS             complement(1417..2133)
                     /locus_tag="VBC23_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842077.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
     gene            complement(2397..3080)
                     /locus_tag="VBC23_000093"
     CDS             complement(2397..3080)
                     /locus_tag="VBC23_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842078.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYCHLPFINIPLWIYLIILGLSFSLHIIVKKY
                     LNKHYYIEF"
     gene            complement(3101..3412)
                     /locus_tag="VBC23_000094"
     CDS             complement(3101..3412)
                     /locus_tag="VBC23_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6842079.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            3603..4310
                     /locus_tag="VBC23_000095"
     CDS             3603..4310
                     /locus_tag="VBC23_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6842080.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            4588..5736
                     /gene="nagA"
                     /locus_tag="VBC23_000096"
     CDS             4588..5736
                     /gene="nagA"
                     /locus_tag="VBC23_000096"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6842081.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            5854..6396
                     /locus_tag="VBC23_000097"
     CDS             5854..6396
                     /locus_tag="VBC23_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6842082.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            6709..7623
                     /gene="glyQ"
                     /locus_tag="VBC23_000098"
     CDS             6709..7623
                     /gene="glyQ"
                     /locus_tag="VBC23_000098"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6842083.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            7623..8264
                     /locus_tag="VBC23_000099"
     CDS             7623..8264
                     /locus_tag="VBC23_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6842084.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            8268..10307
                     /locus_tag="VBC23_000100"
     CDS             8268..10307
                     /locus_tag="VBC23_000100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6842085.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            10319..10576
                     /locus_tag="VBC23_000101"
     CDS             10319..10576
                     /locus_tag="VBC23_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6842086.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            10665..10928
                     /locus_tag="VBC23_000102"
     CDS             10665..10928
                     /locus_tag="VBC23_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6842087.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            11043..12551
                     /gene="glpK"
                     /locus_tag="VBC23_000103"
     CDS             11043..12551
                     /gene="glpK"
                     /locus_tag="VBC23_000103"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6842088.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            12564..14393
                     /gene="glpO"
                     /locus_tag="VBC23_000104"
     CDS             12564..14393
                     /gene="glpO"
                     /locus_tag="VBC23_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6842089.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            14405..15103
                     /locus_tag="VBC23_000105"
     CDS             14405..15103
                     /locus_tag="VBC23_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6842090.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            15191..16457
                     /locus_tag="VBC23_000106"
                     /pseudo
     CDS             15191..16457
                     /locus_tag="VBC23_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            16448..17878
                     /locus_tag="VBC23_000107"
     CDS             16448..17878
                     /locus_tag="VBC23_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842091.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            18003..19988
                     /gene="tkt"
                     /locus_tag="VBC23_000108"
     CDS             18003..19988
                     /gene="tkt"
                     /locus_tag="VBC23_000108"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6842092.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            20200..20505
                     /locus_tag="VBC23_000109"
     CDS             20200..20505
                     /locus_tag="VBC23_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6842093.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            20525..21259
                     /locus_tag="VBC23_000110"
     CDS             20525..21259
                     /locus_tag="VBC23_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842094.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            21263..22867
                     /locus_tag="VBC23_000111"
     CDS             21263..22867
                     /locus_tag="VBC23_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842095.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(23075..24461)
                     /locus_tag="VBC23_000112"
                     /pseudo
     CDS             complement(23075..24461)
                     /locus_tag="VBC23_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            24602..25405
                     /gene="proB"
                     /locus_tag="VBC23_000113"
     CDS             24602..25405
                     /gene="proB"
                     /locus_tag="VBC23_000113"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6842096.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            25415..26668
                     /locus_tag="VBC23_000114"
     CDS             25415..26668
                     /locus_tag="VBC23_000114"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6842097.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            26751..27698
                     /gene="rsmH"
                     /locus_tag="VBC23_000115"
     CDS             26751..27698
                     /gene="rsmH"
                     /locus_tag="VBC23_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6842098.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            27713..28039
                     /gene="ftsL"
                     /locus_tag="VBC23_000116"
     CDS             27713..28039
                     /gene="ftsL"
                     /locus_tag="VBC23_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6842099.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            28043..30301
                     /locus_tag="VBC23_000117"
     CDS             28043..30301
                     /locus_tag="VBC23_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6842100.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            30303..31313
                     /locus_tag="VBC23_000118"
     CDS             30303..31313
                     /locus_tag="VBC23_000118"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6842101.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            31411..32754
                     /locus_tag="VBC23_000119"
     CDS             31411..32754
                     /locus_tag="VBC23_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6842102.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            32892..33704
                     /locus_tag="VBC23_000120"
     CDS             32892..33704
                     /locus_tag="VBC23_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6842103.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            33799..34602
                     /locus_tag="VBC23_000121"
     CDS             33799..34602
                     /locus_tag="VBC23_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6842104.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            34602..35345
                     /locus_tag="VBC23_000122"
     CDS             34602..35345
                     /locus_tag="VBC23_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6842105.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            35467..35691
                     /locus_tag="VBC23_000123"
     CDS             35467..35691
                     /locus_tag="VBC23_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6842106.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            35760..36674
                     /gene="trxB"
                     /locus_tag="VBC23_000124"
     CDS             35760..36674
                     /gene="trxB"
                     /locus_tag="VBC23_000124"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6842107.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            36832..38292
                     /locus_tag="VBC23_000125"
     CDS             36832..38292
                     /locus_tag="VBC23_000125"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6842108.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            38289..39110
                     /gene="nadE"
                     /locus_tag="VBC23_000126"
     CDS             38289..39110
                     /gene="nadE"
                     /locus_tag="VBC23_000126"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6842109.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            39223..40557
                     /gene="pepC"
                     /locus_tag="VBC23_000127"
     CDS             39223..40557
                     /gene="pepC"
                     /locus_tag="VBC23_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6842110.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(40604..42844)
                     /locus_tag="VBC23_000128"
     CDS             complement(40604..42844)
                     /locus_tag="VBC23_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6842111.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(42831..43430)
                     /gene="recU"
                     /locus_tag="VBC23_000129"
     CDS             complement(42831..43430)
                     /gene="recU"
                     /locus_tag="VBC23_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6842112.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            43505..44023
                     /locus_tag="VBC23_000130"
     CDS             43505..44023
                     /locus_tag="VBC23_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6842113.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            44144..44476
                     /gene="gpsB"
                     /locus_tag="VBC23_000131"
     CDS             44144..44476
                     /gene="gpsB"
                     /locus_tag="VBC23_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6842114.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            44495..44863
                     /gene="rnpB"
                     /locus_tag="VBC23_000132"
     ncRNA           44495..44863
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBC23_000132"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            44946..46100
                     /locus_tag="VBC23_000133"
     CDS             44946..46100
                     /locus_tag="VBC23_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6842115.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            46113..47600
                     /locus_tag="VBC23_000134"
     CDS             46113..47600
                     /locus_tag="VBC23_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842116.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(47825..48307)
                     /locus_tag="VBC23_000135"
     CDS             complement(47825..48307)
                     /locus_tag="VBC23_000135"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6842117.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            48496..50103
                     /locus_tag="VBC23_000136"
     CDS             48496..50103
                     /locus_tag="VBC23_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6842118.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            50232..51128
                     /locus_tag="VBC23_000137"
     CDS             50232..51128
                     /locus_tag="VBC23_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842119.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYLEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            51115..51855
                     /locus_tag="VBC23_000138"
     CDS             51115..51855
                     /locus_tag="VBC23_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842120.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            51852..52937
                     /locus_tag="VBC23_000139"
     CDS             51852..52937
                     /locus_tag="VBC23_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6842121.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            52939..53529
                     /locus_tag="VBC23_000140"
     CDS             52939..53529
                     /locus_tag="VBC23_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842122.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            53579..54283
                     /locus_tag="VBC23_000141"
     CDS             53579..54283
                     /locus_tag="VBC23_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6842123.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            54450..55079
                     /gene="gmk"
                     /locus_tag="VBC23_000142"
     CDS             54450..55079
                     /gene="gmk"
                     /locus_tag="VBC23_000142"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6842124.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            55102..55416
                     /locus_tag="VBC23_000143"
     CDS             55102..55416
                     /locus_tag="VBC23_000143"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6842125.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            55490..57880
                     /locus_tag="VBC23_000144"
     CDS             55490..57880
                     /locus_tag="VBC23_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6842126.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            57927..58862
                     /locus_tag="VBC23_000145"
     CDS             57927..58862
                     /locus_tag="VBC23_000145"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6842127.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            58852..60174
                     /gene="rsmB"
                     /locus_tag="VBC23_000146"
     CDS             58852..60174
                     /gene="rsmB"
                     /locus_tag="VBC23_000146"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6842128.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            60212..60949
                     /locus_tag="VBC23_000147"
     CDS             60212..60949
                     /locus_tag="VBC23_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6842129.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            60949..62904
                     /gene="pknB"
                     /locus_tag="VBC23_000148"
     CDS             60949..62904
                     /gene="pknB"
                     /locus_tag="VBC23_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6842130.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            63063..63758
                     /locus_tag="VBC23_000149"
     CDS             63063..63758
                     /locus_tag="VBC23_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6842131.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            63755..64774
                     /locus_tag="VBC23_000150"
     CDS             63755..64774
                     /locus_tag="VBC23_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6842132.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            64767..65408
                     /locus_tag="VBC23_000151"
     CDS             64767..65408
                     /locus_tag="VBC23_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842133.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            65450..66850
                     /locus_tag="VBC23_000152"
     CDS             65450..66850
                     /locus_tag="VBC23_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6842134.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            66852..67208
                     /locus_tag="VBC23_000153"
     CDS             66852..67208
                     /locus_tag="VBC23_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6842135.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(67254..68030)
                     /locus_tag="VBC23_000154"
     CDS             complement(67254..68030)
                     /locus_tag="VBC23_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6842136.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            68151..68906
                     /locus_tag="VBC23_000155"
     CDS             68151..68906
                     /locus_tag="VBC23_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6842137.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            69025..70008
                     /locus_tag="VBC23_000156"
     CDS             69025..70008
                     /locus_tag="VBC23_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6842138.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            70212..70535
                     /locus_tag="VBC23_000157"
     CDS             70212..70535
                     /locus_tag="VBC23_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6842139.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            70552..70872
                     /locus_tag="VBC23_000158"
     CDS             70552..70872
                     /locus_tag="VBC23_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6842140.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            70874..72175
                     /locus_tag="VBC23_000159"
     CDS             70874..72175
                     /locus_tag="VBC23_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6842141.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            72356..74812
                     /locus_tag="VBC23_000160"
     CDS             72356..74812
                     /locus_tag="VBC23_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6842142.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            74822..75490
                     /locus_tag="VBC23_000161"
     CDS             74822..75490
                     /locus_tag="VBC23_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6842143.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            75558..76646
                     /locus_tag="VBC23_000162"
     CDS             75558..76646
                     /locus_tag="VBC23_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6842144.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(76798..77724)
                     /gene="cysK"
                     /locus_tag="VBC23_000163"
     CDS             complement(76798..77724)
                     /gene="cysK"
                     /locus_tag="VBC23_000163"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO6842145.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(77815..78459)
                     /locus_tag="VBC23_000164"
     CDS             complement(77815..78459)
                     /locus_tag="VBC23_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6842146.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            78515..79804
                     /locus_tag="VBC23_000165"
     CDS             78515..79804
                     /locus_tag="VBC23_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6842147.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            79804..80470
                     /locus_tag="VBC23_000166"
                     /pseudo
     CDS             79804..80470
                     /locus_tag="VBC23_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            80547..81101
                     /gene="raiA"
                     /locus_tag="VBC23_000167"
     CDS             80547..81101
                     /gene="raiA"
                     /locus_tag="VBC23_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6842148.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT        26594 a        12818 c        16236 g        25601 t
ORIGIN      
        1 tgaattagtt cctctttagt agaaaatgta gataattctg taaagaaggg gcgtccaata
       61 gtttgtacga ttaactcgag aataaagacc aaaagcttgt ctttagtttc aaaatattta
      121 tatatagttg cttggctaac ttctgcattt ttagctagtt gagcagtgga agttccatgg
      181 aagccttgtg aggcaaagag ctctatagcg gataaaataa ctttttgttt tccttttggc
      241 atctcttggt cattaatata ctgttcaata gaaaaaataa tattattttt catacttagt
      301 tccttttcaa atggtattat actttacggt atcgttttag tccaaagata tttgcgatgg
      361 ttaaaataat taaaaataga agaagtacca gaagatttga agaaacgtta ggtaatcctt
      421 gaccgtacat aattattttt gtaagtgcat caccagagta ggatagcggt agaattttac
      481 cgactgtctg agcccaactt gccatatttt ctagagggat tattcctgaa aagaataatt
      541 gaggcattat aattaaggga ataaattgca tcatttgaaa ttctgatttt gctagagtag
      601 acattaagat tcctaatgat aaggcaacca gagccaaaat aaaattgaca atgatgacac
      661 taaaaatact acctacaact tgaatatcta gtagccaaat agttgataaa acgataacta
      721 tcgtttgaat aattgctaga atgccgtatg ataacatata tccaaataca atatcactac
      781 gtttaacagg agttgcaagg agtcgatcaa gtgtaccaga agtacgctct tttaataaag
      841 ccattccaga aattaaaaaa acaaagaaga agaccataaa tcccattagt attggtatca
      901 tttttgcaaa aaagcctgta tttttatttc cgtaattata tttttctttg atatatttgg
      961 agcgagtttg taaggattgt gctagtttcg gattcttatt agctaaaatt ttaacttgcg
     1021 aaatcagttc cttactgttc attgtattaa cagcggtttt aaaagcttgt cttgttaaag
     1081 tcgtctttga agaatctgta ttcgcataga agacagtata agatttattg tcctccgaaa
     1141 taagagcatc aattttattt gatttgagtg cttttttagc agatgagtta aatttaaatg
     1201 atctcacttg aatatgctta atattatcta aatttgaaac gaccttcgtg ttaacgttaa
     1261 tagttccaat cttaactttt gtattactat tcgcagaaaa cataacattc atcaaaaaca
     1321 taattaaaat aggtgctaaa aacatcatag caagtgttct tttatcacga aacagttctt
     1381 ttataacctt ttcagttatt gcaataattc tcatgattat tctccttcag cttttaagaa
     1441 aacttcctca atagtactca cattaaattg tttttttaaa tgtaatggag tatcaaaggc
     1501 aataatgttt ccacgtaata gtagtgcaac cttacttgtt aattctgctt catccataac
     1561 gtgggttgta ataaagatag aacgtccttc atccttaata ttaattagct cttgccagat
     1621 ttttctcctc aaggatggat caattccaac ggtaggttca tctaggatta aaactgtggg
     1681 gtttccaagt agggcgatgg ctagagaaag ccgtcttttc atacctcctg agtaacctga
     1741 gacaaattta tcaagttggt tttctagatc tactacttta gaaatatgag ttatctgctg
     1801 ttttaattca gttttttgaa tacctttcat ttttccaaag aataataaat tttctaagcc
     1861 agttaaagac tcgtgtaagg catcagattg agccatatag ccaatttgat ttaaaatatt
     1921 acgatctggc atttgagtat caagaacaag agctgttccc ttatctgctt tttccatgcc
     1981 aagcatagtt ttaatcaagg tagatttccc tgctccagag ggtcctatta atccaattat
     2041 ttctccttta aacacctcca aattaatatt atttaaaaca gtttctgagg cgtatgcttt
     2101 ttgtagtttt tttaaatcga tgactttttt catgataact ttttgctcct ctctcagtga
     2161 gtgagtgttt actcaccaat ttgatatcat atattatagt gtgtaaagac aggcttgtca
     2221 ataaaagaat tgacaggaag ttaatatact ataatagtca ataaaatggc ttatttgaga
     2281 taactaattt tttatcttaa aaatcagttg catggagagc attcgtgaaa atgggcacag
     2341 aataaaacga ctaagaatat ccttgtcgtt ttatttttta tatagttata cctcttttaa
     2401 aattcaatat aataatgttt attcaggtat tttttaacaa tgatatgaag agagaagcta
     2461 agtcccagta taattaaata aatccaaagg gggatattaa tgaaaggaag atgacaatat
     2521 tttcctataa ggatataagc tatgataagc gaaagaatta gaaacatata tcgaaagaaa
     2581 tatttctttt ctttcgattt atcgaagtta tatgtagcat atattatggt atcagttatc
     2641 ttgacagcaa taatactgat aaagattgca aaaagaacat ctaatagagt aatagggatt
     2701 agatctggta ctgactgttg aagtgcttca gtaatgagat atcctcctaa aaagataatc
     2761 atttggatag ttagtagtaa aatggctaaa tcccaacgtt gtaatgtttc ttctttctta
     2821 gagattttag gtgtagattt aataacttgg tttataaagt cgtgcctatc ttctccaaat
     2881 acactaacta aacttgcttg tcgctcttgt gcttctaata acatattgac aatatcattt
     2941 cgaatgagct cctggtggta ggggctaatg ctagcacctc gcaaatagac aatcatatct
     3001 gtataggttt ggttattaga gttatcaatt cttccttcta atagcgtatt ttctttttgt
     3061 aattttttca gtttatccat gctagtttcc tctccaattt ttagagtagt ttgtttaatg
     3121 ctttactaag tgactcccaa ttctgtttaa attgggctaa ttgctccacg ccttcattgg
     3181 ttaacgtata atatttacgt ttaggtcctt gaggggaagg cttttgttgt ccttttatat
     3241 atttattgct ttgtagcctt aagataatgg ggtagattgt cccttcggaa atttccccaa
     3301 aaccataaga atttaatttt tttgagattt cataagcata agtctcctct tgctgtatga
     3361 tttttaggat agctccttca agtaatcctt ttagcatttg cgatggaatc atattatatc
     3421 cttatctata ttgtaataca agttactggt taactacatt gtaatacaaa gtagttgaga
     3481 tgtcaaatat aactaagtta ccggtagaaa ataagagtga tagacgatga ttcttctaaa
     3541 ataggtttgt ttatgtcctt tagtttatgt tataataatg tgaagaaact ttaggagtga
     3601 gtatgaaaat agataaaaaa gaacttttag cacttattgc tagtattatt ttattaattt
     3661 ttgcttctgt tacgtttttc ttatttaaag atcatggcac tactcaaatg gatactgtag
     3721 aaagcagtgt taaccatgtc agtgattctc aattaactga ggcacaagat atgttagata
     3781 aatttgagaa gaaaccttca gaaaaattat tgaaggatgt cgaacttgct ttaaataaat
     3841 tgtccaattc ttctaaaaaa gaagctttgc aaaaacgttt taagaaagca aaagataagt
     3901 atcttaaaga cgaagcagat aagaaagcta ccaaagatgc tacagattta gtagaaattc
     3961 tagaacaagc cccttcagaa gaaaatgtgt taaaagctga agcagcagtc aaaaagttaa
     4021 ctgtcaaaga atctaaagaa gctttacaaa aacgtattga tactgttaag acacaatatg
     4081 gtttgattgg gaatcaaact ccctctagtt cagtagctga aactacagaa caagggacag
     4141 ctaatcctgc tagtcaggat acttctagtt acgttaatca ggatgtagca ccaacttatg
     4201 agcaaccgca aacgaataat acaccagtta ctccaggggt taacaatact gttccgactc
     4261 caggaactgg tactgtacct gctactaatg ggacgggtgt tgctcagtaa aatcctgaaa
     4321 gtctagtttt actagacttt tttgctatgc tttttaatta gccattctca tcaaaaacta
     4381 taagagaatt agcaattgat agttttggag aaggaaaaga attatgagca atagcaatta
     4441 aaaccttaaa agtacaacca agtgaagtgg tttataacaa gtgtatagtt ctttcttaga
     4501 ataaaccatg actttttaaa aaataggtat ataccattta caaaaatacc ttaaagcgtt
     4561 ataatgagag tgaatggggg gaagaagatg acgaagtaca tcaaggctga ccgattcttt
     4621 tatgctgatc atgtaaagga aaacggttat ttagaaataa aagataatca ctttggtaaa
     4681 tggatagaaa atatttctgg acaagaggag attttagatt attcaggtta ccaaatagca
     4741 cctggtttag ttgatacaca tattcatggt tttgctggcg ctgatgttat ggattgtgat
     4801 tcagaaggaa ttttgcgaat gagtgctggc ttactatcta ctggagtcac atctttcttg
     4861 ccgacaaccc taacttcaga tacaaagcga ctagaagagg cgtctaaagc agttgcggca
     4921 gtagcgggca aagaacaagg agcaaaaatt caaggcatct attttgaggg accatatttt
     4981 actgaggaat ataaaggagc tcaaaatccg atttatatga gaaatccaaa cttggaagag
     5041 tttgctcaat ggcagaaggc tgcaaaaggc ttgattacaa aaattgcttt ggcaccagaa
     5101 cgtgagggag ttgaggaatt tgtctcagca atcacaaagc aaggtgttac ggtagcttta
     5161 ggtcattcta atgggacata taaagaagct aaaaaggcag tgaaggcggg tgcctcagtt
     5221 tgggtacatg cgtataatgg tatgcgtggt ttgacacacc gagaaccagg tatggttggt
     5281 gcggtttata atttacctaa tacttatgct gaactcattt gcgatggtca tcacgttgat
     5341 cctgttgctt gtgacatttt aatgacacaa aaaggccata atcatgtggc attaataaca
     5401 gattgtatgg cagctggagg tgcccctgat ggagattata tgttagggga attgcccgtg
     5461 gttgtgtcta atggaactgc gcgtttgaaa gatggaggga atttagctgg ttctattctt
     5521 aagctcaaag atggattaaa aaatgttgta gaatggggaa ttgctactcc ggaagaggca
     5581 gtgcgtatgg cgagttatgt tccagcagtg tctgtaggca ttgataatgt ctgtggacag
     5641 ataaaagaag gctatgcagc ggattttatt gtattagata aacatctaga tttagttgca
     5701 acataccttg atggagaaaa acgttggcaa tcttgaaaga actagttttt actagttctt
     5761 tttaatttat taatttttaa tgaaatatag tttgacaata aaactatttt tcttataatg
     5821 ttgtaatata atacaaaaca ataggagttt acaatgtcaa ttattattgg gttaatggcc
     5881 gctatgttta tcattcgttt agcttattta aagctatcta tagctaatga aaaggcctta
     5941 agaaaaaatg gtgctaaaga atatggtgta ggtgtttcaa aagcaatcac agttcttcat
     6001 attatcattt atttttcatc tgtaactgag gctgttctaa caaaagcaag ttttaatttt
     6061 gttagcgtaa tcgggcttag cttgatgatt ttttctgtgt ttatgttaca tacagtaaca
     6121 cgtttacttg gtcgtatttg gacagtgaaa ttaatggtag ataaaaatca tcaatttgta
     6181 gatcactggc tatttagggt tgttaagcat cctaactatt tcttaaatat tgctcctgaa
     6241 cttttaggtg taactttatt gtgtcatgct aagtatacag cattatttgt cttgccaatt
     6301 tatgcttttg ttatttattt aagaattcgt gaagaaaact tattattaaa aacgatcatt
     6361 attccaaatg gaatcaaaaa gtctagggta tcttaagaat aagataaata gtagacataa
     6421 aagtcatttt ttgttatact gtctcagtaa gaagaggtgt tcatcagctg tttttttagc
     6481 gagctcaagg tagtgaaagt tgggtagaat aggatgaata ttggcacttt ttagtgagta
     6541 aaagtacaat caaaaatgaa gtaataaatt agggtggaac cgcgtctttg acgcccctgt
     6601 gctgttgggc ataggtggtg taggacgtgg tttttatcat ttgtccgttt taagtcaatt
     6661 gttgtcacct tcccaacagc tgtaataaag tatgaaagaa ggaaaatgat gtctaagaaa
     6721 ttaacttttc aagaaattat tctaacttta caacagtttt ggaatgacca aggctgtatg
     6781 ttaatgcaag cttatgataa tgagaaagga gcagggacaa tgagccctta tactttctta
     6841 cgtgctatag gccctgagcc ttggaatgct gcatacgttg aaccatcacg tcgtccagct
     6901 gatggccgct atggtgaaaa tccaaaccgt ctttaccaac atcatcaatt tcaagtggta
     6961 atgaagcctt cgccgtcaaa tattcaagaa ctttatttga agtctttgga attactaggt
     7021 atcaatccac ttgagcatga tatccgtttt gttgaagata actgggaaaa tccatctaca
     7081 ggttcagctg gattaggttg ggaagtttgg cttgatggaa tggaaataac acaatttact
     7141 tatttccaac aagtaggtgg acttcaaaca ggtcctgtaa cttctgaagt tacttatgga
     7201 ctagagcgtt tagcctctta tattcaagag gtagattctg tttatgatat tgagtgggca
     7261 ccaggtgtta aatatggtga aatctttact cagccagagt atgagcattc aaaatattca
     7321 tttgagatat cagaccaagt aatgttactt gaaaactttg agaaatttga aagagaagca
     7381 aaacgtgctt tagaagaagg acttgttcat ccagcttatg actacgtcct taaatgttca
     7441 cacaccttta atttattaga tgcacgtgga gcagtatctg ttactgagcg tgcaggctat
     7501 attgcacgta ttcggaattt agcacgtgtt gttgctaaaa cctttgttgc cgaacgtaaa
     7561 aaacttggtt tcccactttt ggatgaggaa actcgtatca aattacttgc tgaggaagac
     7621 taatgaacac actcattgtt aatagtcatc ctgatttttc gaatccgtac tcatttacaa
     7681 ctattctaca agaaaagttt attgaacttt ataatgagca tttccctaac catcagttat
     7741 ctattttaaa tttatatgat tgtgttttgc cagaaattac gaaagaagta ctactttcga
     7801 tttggtctaa acaaagaaag ggtttggaat taacagccga tgagattgtt caagctaaaa
     7861 tctctaaaga tttacttgaa cagtttaagt cacatcaccg tattgtcttt gtttctccaa
     7921 tgcacaatta caatgtgact gctagagcaa aaacttatat tgataatatt tttattgcag
     7981 gtgagacttt caagtacact gaaaatggtt cggtcggatt aatgacagat gactatcgtc
     8041 tgcttatgtt agaatctgcg ggttctattt attctaaagg acaatatagt ccatatgaat
     8101 ttcctgttca ttatttaaaa gctatcttta aagattttat ggcttttgat gatttctgtg
     8161 ttgttcgtgc agaaggtacc gatattttag ataggcaagt tgttcttgat aagatgaatc
     8221 aggatttaag agaagcattt gaagcgtttt atagtaagga gtaggaaatg actaaagatt
     8281 tattattgga gcttggactt gaagagttgc cagcttatgt tgtgacaccg agtgagaagc
     8341 aattgggcca aaaaatggtc aagtttttag aggatcatcg tttatcattt gaaactgttc
     8401 aaactttttc aactccacgt cgtttggctg ttcgtgttaa aggattggca gaccagcaga
     8461 cagatttaac agaagatttc aagggtcctt ctaagaaaat tgctttggat gcagaaggga
     8521 atttttctaa agcagcccaa ggttttgttc gcggaaaagg tttaagtgtt gatgatatcg
     8581 aatttcgtga agttaaggga gaagaatatg tttatgtcac taagcatgaa actggaaaaa
     8641 gtgcgattga tgttttagcg agtgtgacag aggtattaac agaattgact ttcccagtga
     8701 acatgcattg ggcaaataat agttttgaat atattcgtcc agttcacact ttagtggtat
     8761 tattagatga tcaagcctta gagctagact tccttgatat tcattcaggg cgtattagtc
     8821 gaggacaccg tttcttgggt tctgacactg aaatttcatc tgccagttcg tatgaagatg
     8881 acttgcgaca acaatttgtt attgctgatg caaaagaacg tcagcaaatg attgttaatc
     8941 aaattcatgc tatcgaagaa aagaaaaata tttcagttga aattgacgaa gatttactaa
     9001 atgaagtgct taatttagtt gagtatccga cagcattctt gggttcattt gacgagaaat
     9061 accttgatgt acctgaagaa gttttagtca cttcaatgaa aaatcatcaa cgctattttg
     9121 ttgttcgcga cagggatgga aaattgttac caaactttat ttctgtgcgt aatggaaatg
     9181 ctgagcatat cgaaaatgtt ataaaaggca atgaaaaagt tttagtggct cgtcttgaag
     9241 atggtgaatt cttctggcaa gaagatcaga aacttaatat tgcagattta gttgagaaat
     9301 taaaacaagt aactttccat gaaaaaattg gttcacttta tgaacatatg gatcgtgtaa
     9361 aagttatttc acaacatttg gctgataagg caggtctttc tgatgaagaa aaattagctg
     9421 tgcttcgagc tgcaagtatt tataaatttg acctcttaac tggaatggtt gatgagtttg
     9481 atgaattaca aggtattatg ggtgagaaat atgccttact tgcaggggaa cagcccgcag
     9541 tagcagcagc gatacgtgaa cattatatgc cgacatcggc cgatggagag cttccagaaa
     9601 ccagaattgg tgcaattcta gcgttagctg ataaatttga caccttactt tcattcttct
     9661 cagttggttt gattccaagt ggttcaaatg atccatatgc tcttcgtcga gctactcagg
     9721 ggattgtccg tattttggaa gcttttggtt gggatatccc acttgatgaa ttagtaacta
     9781 acctttatgg tctatcgttt gctagtttag attatgctaa tcaaaaagag gtcatggcct
     9841 ttatttcagc tcgtattgaa aagatgattg gctctaaggt ccctaaagat attcgagaag
     9901 ccgttttaga atcagacact tatattgtta gccttattct agaagctagc caagcattgg
     9961 tgcaaaagag caaggatgct caatataaag tttccgttga aagtttgtca cgtgcattta
    10021 atttagctga aaaagttact cattctgtat cggtggactc ttcattgttt gaaaataatc
    10081 aggagaaggc gctatatcaa gctattttgt cacttgaact gactgaggat atgcatgaca
    10141 acttagataa gcttttcgca cttagtccaa ttattaatga tttctttgat aatacaatgg
    10201 taatgacaga tgatgaaaaa atgaaacaaa atcgtctggc tttactcaat agccttgttg
    10261 caaaggcaag aacagtcgct gcttttaact tattaaacac aaaataagga ggcattttat
    10321 ggatcctaaa aaaattgctc gtattaacga gctttcaaaa aagaaaaaaa cagttggatt
    10381 gaccggagaa gagaaggttg aacaagcaaa gctccgtgaa gagtatattg aaggtttccg
    10441 ccgatcagtc cgtcaccacg ttgaaggaat taaattagtg gatgatgagg gcaatgatgt
    10501 taccccggaa aaacttcgtc aagtccagag agaaaaagga ctacatggtc gtagtttgga
    10561 tgatccaaat tcttaatggt gagacaattt taaattaaag tcaacacttg ggtggggcaa
    10621 aagataatct ttgtctcgcc ttttttatta ggaaggaaaa aataatgatt gaattactat
    10681 tagcattact gttatggaat attttagtat ttgcaaccta tgctattgat aaaaggcgtg
    10741 caattagagg ggcatggcgt attagtgaga aaacattgtt gatagagagt ttattattag
    10801 ggggatttgg tgcattccta ggtggagaag tttttcgaca taaaacgact aaatggtatt
    10861 ttagactagt ttggtatatc ggaatgatta ttgatttagc tattatttgg ctaatttggt
    10921 gttattgatg atatcttatc aaaaatttta ggggcatatt aaatttttga tagtgcagga
    10981 tagactgatt taggtataat agaattacaa aaagaaaacg cttactttta aggaggaagt
    11041 ttatgtcttc agaagaaaaa tacgttatgg cgattgacca gggaacaaca agttctcgag
    11101 caattatttt taataaaaaa ggtgaaaaaa ttgcgagtag ccaaaaagaa tttccccaaa
    11161 tttttcctca agcaggctgg gtagagcata atgctaatca gatttggaat tctgttcaat
    11221 ctgtcattgc tggagctttt attgaatcaa gcatcaaacc tggacagata gaggctattg
    11281 gtattaccaa tcaacgtgaa acaactgttg tttgggataa aaaaacaggt cttccaattt
    11341 ataatgctat tgtatggcaa tcgcgacaaa cagccccgat tgctgatcaa ttgaagcaag
    11401 aaggtcatac taatatgatt catgaaaaaa ctggtcttgt cattgatgct tacttctcgg
    11461 caacaaaagt tcgttggatt ttagaccatg ttcctggagc acaggaacgt gctgaaaaag
    11521 gagagttgct ttttggaaca attgatactt ggttagtttg gaagttaact gacggtctcg
    11581 tccatgttac agactattca aatgctgctc gtacgatgct ttataatatt aaagaactta
    11641 aatgggatga tgaaatttta gagttactga atattcctaa agcaatgtta ccagaagtaa
    11701 aatctaattc tgaagtttat gggaaaacta ctccattcca tttttatggt ggagaggtgc
    11761 caatttctgg tatggctggt gaccaacagg cagcattatt tggacagttg gcctttgaac
    11821 ctggtatggt aaaaaacact tatggtacag gttccttcat tatcatgaat acgggcgaag
    11881 agatgcaact gtctcaaaat aatctattaa caacgattgg ttacggcatt aatggtaagg
    11941 ttcattatgc acttgaagga tcaatcttca ttgcaggaag cgctattcaa tggttacgtg
    12001 atggtcttag aatgattgaa acgtcaagtg agtcagaagg ccttgctcaa tcatcaacaa
    12061 gtgacgatga ggtttatgtg gttccagctt ttacaggttt gggtgcaccg tattgggatt
    12121 caaatgctag agggtcagta tttggattaa ctcgtgggac aagtaaagaa gattttgtta
    12181 aagctacact tcagtcaatt gcttatcaag tacgagacgt tattgacact atgcaagttg
    12241 attcaggaat tgatatccaa caacttcgtg tagatggtgg agctgcaatg aataatttat
    12301 taatgcaatt ccaagcggat attttaggga ttgatattgc tcgtgcgaag aaccttgaaa
    12361 ctactgcctt gggtgcagct ttcttagccg gactttcagt tggctattgg gaaagtatgg
    12421 atgaactaaa agaactgaac gcaacaggac aacttttcca agcaacaatg aatgagtctc
    12481 gaaaagaaaa actgtataaa gggtggagaa aagctgtcaa agcgacacaa gtatttgctc
    12541 aagaggatta agggggtgta gagatggaat tttcacgaga aacacgacgt ttagccttac
    12601 aaagaatgca agatcgtact ctggatttac ttatcatagg tgggggtatc acaggcgctg
    12661 gtgtcgcttt acaagcagca gctagtggat tagatactgg acttattgaa atgcaagatt
    12721 ttgcagaagg gacaagtagt cgctcaacaa aattggtcca tggtggtctt cgttacttga
    12781 agcaatttga tgtagaagtt gtttctgata cagtatcaga gcgtgcagtc gtgcagcaaa
    12841 ttgcaccaca cattcctaag ccagatccaa tgcttttacc ggtttacgat gagccgggtt
    12901 ctacttttag tatgtttcgc cttaaagtgg ctatggattt atatgattta ttagcaggtg
    12961 tgacaaacac acccgcagcc aataaagttt taagcgcaga agatgttctt aagcgtgagc
    13021 ctgatttaca aaaagaaggt ttgctaggcg gaggggtcta tttagatttc cgtaataacg
    13081 atgcacgcct agtgattgaa aatattaaac gtgctaatag ggatggagct tatattgcca
    13141 gtcatgtcaa agctgaggac ttcttatttg atgacaacaa tcaaattata ggagtacgtg
    13201 cgcgtgattt gttgactgac caagttatcg atataaaagc tagacttgtt attaatacaa
    13261 ctggaccttg gagtgacacc gttcgcaact tctccaatga aggtaaacaa attcatcaat
    13321 tacgtccaac taagggagtt cacttagttg tagatcgcca aaaattaaat gtttctcaac
    13381 ctgtttatgt tgatacaggt ctaaatgatg gtcgcatgat ttttgttttg ccacgtgaag
    13441 ataagactta ttttggaacc acagatactg attaccatgg agacttagaa catcctacgg
    13501 taactaaaga agatgttgac tacctcctta atattgtaaa caaacgcttc ccagaagcag
    13561 agcttactat tgatgatatt gagagttcat gggcaggtct gcgtccacta ctatcaggga
    13621 atagtgcctc tgactataat ggaggcaata gtggaaaact aagcgatgaa agttttgaag
    13681 aattaattga ttccgttaaa gattatattg cacataaaaa tcatcgtgag gatgttgaaa
    13741 aagctatttc tcatgtagaa tctagtactt ctgaaaaaga attggaccca tcagctgttt
    13801 ctcgaggatc aagttttgag cgtgatgata atggcctatt aacacttgct ggtggtaaaa
    13861 ttactgatta tcgaaaaatg gcagaaggtg ccatggaaac tattattaac attttggata
    13921 aagaatataa tcgcaagttt aaactaatca attctaaaac ctatcctgtt tcaggaggag
    13981 agattaaccc atctaatgtt gattctgaaa ttgaagctta tgctcaattg ggcacactaa
    14041 gtggtttaag tattgaggat gcacgttata ttgctaacct ttatgggtca aatgcaccaa
    14101 aattatttgc attgacacgc caaattacag aagcagaagg tctgagcttg gtagaaacgt
    14161 tgtcacttca ctatgctatg gattatgaaa tggcgcttag cccaacggat ttcttcttaa
    14221 gacgtacgaa tcatatgctt ttcatgagag ataatttaga ttctttgatt cagcctgtta
    14281 ttgatgagat ggctaaacat tatcaatggt ctgatcaaga caaaactttc tatgaagaag
    14341 aattacatga gactctaaaa gacaatgact tggcagcttt gaaggataat tgaaggagat
    14401 aataatggat atttttggag aattcttagg aacagctctc ttagtcttac ttggtaatgg
    14461 tgttgtagca ggtgttgttt tgcctaaaac gaagaatcat aattctggat ggattgttat
    14521 cacatttgga tgggggcttg ctgtagctat tgcagcgttg gtatctggca atattagccc
    14581 tgcccacttg aatccagctg tgtcattagc ttttgcgatc aaaggtgatt tagcttgggg
    14641 aacagctatt ctttatatga ttgcacagat tattggtgca atgctaggtt cacttcttgt
    14701 ttatttgcaa tttagacccc attatgaggc tgcagaaaat aaagcagata tccttggaac
    14761 ttttgcaact ggtccggctt tgaaggataa tttttccaat tttttaagtg aagtattggg
    14821 aacacttgtt cttgtactta ctatttttgc catcggaaaa tacaatatgc ctccaggagt
    14881 tggtacgatg agtgttggta tgttagtggt tgggattggt ctatcgctcg gtggaacaac
    14941 aggttatgct attaatcctg cacgtgattt tggtcctcgt ttattgcatg ccttgttacc
    15001 gatgaagaac aaaggtgatt ctgattggac ttattcatgg attccgatag taggacctat
    15061 ggtaggagct attttagcag ccttaatatt tgctatgatg taagagattt ttgatgctga
    15121 gaccgacttt atggtctcag ttttttgtta ccgttttcaa aaataacttc ttttgttata
    15181 ataagtaatt atgacagaaa aatatgtaaa taaagtaatt gtcattttag gagcttcttt
    15241 tgctggtatg acttgtgcgc agaaattacg ccaattaaat cctaattggg atatcgtttt
    15301 gattgataaa gaaattcatc cagattatgt tccaaatggc ttaaattggt attatcgtca
    15361 cgaaattagt ggtttaaatc aggccatgtg gcagacagaa gaagagcaac ggttgcagaa
    15421 tatacgctgt ctattatgat caattgattt gtgccatggg ctcccaagca gaatcaactt
    15481 atattgatgg ggctgatgcg caaggtgttt taacaacaaa aacttatgca actagtcaaa
    15541 atgctaagca ggtattagat aaaagtcata aggtagcagt tgttggggca ggtatcatcg
    15601 gtttagatat tgcttatagt ttacatgaat cagggaaggc ggtcactttg ttagaagctc
    15661 aagaaagacc agacttcagg catacagatc ctgatatgtc ccttccttta cttgatgcga
    15721 tggctgaatc aaaactacat tttttccaaa atcaaaaggt tgagaaaatt actgtaaccc
    15781 gagaagaaaa actttgtttg cgaacgctaa caggagacac ctttacagtt gatactgtca
    15841 tattagctgt taattttcgt ccggatagtc gtcttttaac tgggttggtt gatttaagtg
    15901 ttgataatag tgttgttgta aatgattatt tccaaacgag tgaccctaat atctatgcta
    15961 ttggcgattt gatttggtcg tattttaaag gtcttaatag tgcttattat atgcccttaa
    16021 ttaaccaagc aatccgttcg gcacaaatgt tggcatacca tttatcggga catgctgttc
    16081 caaaattaaa aataacacgc gctacaggat ctaaacactt tggctattat agagctaaca
    16141 ttggattaac tgagctggaa gctggtcttt acgaggatac cgttagtgtt acttatttcc
    16201 ctaaagaaca gtatgattta aggataaaat tgattgctaa tcaaaaaacg ggccatttat
    16261 tgggggctca attaatctca aaggaaaatt gtttggctac tgccaatcag cttgttcaag
    16321 ctatatcgtg tgacatgaca gattttgatt tggcttttca agatttcatt tatacagcta
    16381 gagaatctga gatggcttat atgttacatc aagcagctat caacctttat gaaaagagga
    16441 ttgggctatg cgaatagaag ctctaatgga aaaagaaagg cgtgttcaat atcggctact
    16501 ttctttttta agaggctctc ctcaggcaat agcactgaag ttagctttat tagaaactgg
    16561 tttaagtcga gcaactttgc ttaaatatat caataatctt aatagctact ttgaacaaga
    16621 aaaagttaat tgtcgaatcg tttattataa ggataagtta tttttagaag aggattataa
    16681 cttatccaat caagaagtgt tgaaagctct gatgaaagat agtattaaat acactatctt
    16741 aatatcttta tttaatcagc gtcagttcac aatcgttggt ctttctcaag aattgatggt
    16801 tagtgaggct actttaaatc gtcatcttgc tcatctcaat gaattgttag cggaatttga
    16861 tattgctatt tcacaaggaa aacagattgg tgatgaattg caatggcgtt atttttatta
    16921 tgaattattc aagcaactat ggtcttatga caagtgccaa aatatgataa agaaattaga
    16981 cttagactca ttaattctat taatagagcg cttggctcaa catactttaa ctcgagaagc
    17041 tcagcaaaat ctggggcttt ggtttagtat ttgtcatcat cgtctccttg ctatggaaaa
    17101 actatctgat aatctcaagc ccattgtgga acattatcaa tgtaatgctt tttataaacg
    17161 gttagacgca gccttagttt tgtatatgag tcgttttgct cttgagtata gggaaggaga
    17221 agttctggca acttttgctt ttctacattc acaaaatatt ttaccaatca atacaatgga
    17281 atacatcatg ggatttggag gcccgattat tgattgtgta accgaaacaa tcatctactt
    17341 taaaaaggag agtattttag ctgacgagac atcagatcag gttatttacc aacttggtca
    17401 gttatatagc cattattatt tctttaaagg tcatatttta gttgaacaac ctgatttaga
    17461 acaaacgtat cgacttattg atcataatat gagggataag ttgcatgata tttcaaagaa
    17521 gattattgca aatgttaatc gaattagacc tttaacagaa gatggttgtt ctttgctgac
    17581 tttacatttg ttggaattgc ttattttctc gaaaaatagt caaaaaatgc cctttaggat
    17641 tggcctagat atgacaggta atgctgtgga acagtcattg ttggaatatc gcattcgaca
    17701 acatttctct ggtaataatt ctattcaagt cgaaccttat gacgaaggaa agggatttga
    17761 tatggttatt tatcagagtc gctcaagacc ttataaagcc aaactaacct attgccttaa
    17821 taaaggtgct tcagagcgag aactacagga aatagattca cttatttatg acaattaatc
    17881 ccattactta attgaatggt tttaattcct ccagaaattg ttaaaacgtt tacgtatttt
    17941 atgttataat atcaaagacg aacaatttta aatttagttg ttaaaaacag aaaggtttta
    18001 tcatgacatt tgacaccatt gatcaattag cggttaatac agtccgcacg ctttctattg
    18061 atgctatcca agcagcaaat tctgggcacc caggtcttcc tatgggagct gcgcctatgg
    18121 cttatgtgct ttggaataaa ttcttaaatg taaacccaaa aacaagtcgc aattggacaa
    18181 accgtgaccg ttttgtactt tcagctgggc atggttcagc tcttctttat agcctacttc
    18241 atttagctgg ctatgattta tcaattgatg atttaaaaca attccgccaa tggggatcaa
    18301 aaacacctgg acatccagaa gttaaccata ctgatggtgt agaagcaaca actggacctt
    18361 tagggcaagg tattgctaat gctgttggta tggcaatggc tgaagctcat ttggctgcta
    18421 aatttaataa accaggcttt gatttagttg atcattacac ttatacactt catggtgatg
    18481 gttgtttgat ggagggtgtt agccaagaag ctgccagtct tgctggacac ttaaaacttg
    18541 gtaaattggt ccttctttat gattcaaatg atatctcact tgatggtcca acgtctcaat
    18601 catttacaga agatgttaaa gggcgttttg aatcatatgg ttggcaacat atcttagtta
    18661 aagatggtaa tgatttagaa gcgattgctg cagcaattga agctgctaaa gctgaaactg
    18721 ataaaccaac cattattgaa gttaaaacta ttattggatt tggcgcagag aaacagggaa
    18781 catcttcagt tcatggagcg ccacttggtg cagaaggtat tacttttgcc aagaaggctt
    18841 atggatggga atatccagac tttactgttc ctgctgaagt tgcagctcga tttgcctcag
    18901 atcttcaagc gcgtggtgcg aaggcagaag aggcatggaa tgatcttttt gctaaatatg
    18961 aagttgagta tccagaatta gcagctgaat acaaagaagc ttttgctggc caagctgaga
    19021 cagttgaatt gaaagctcat gacctaggtt catcagtggc tagtcgtgtt tcaagccaac
    19081 aagctattca acaattatcg actcaattac cgaacctctg gggagggtca gcagacctct
    19141 cagcttctaa taacacaatg gtagcagcag aaacagattt ccaagcaagt aactacgctg
    19201 ggcgtaacat ttggtttggt gtacgtgaat ttgcaatggc tgctgcaatg aatggaattg
    19261 cgcttcatgg tgggactcgt gtttatgggg gtacattctt tgtcttctca aactacttgt
    19321 taccagcagt acgtatggct gcacttcaaa acttgccaac tgtctatgtt atgacacatg
    19381 attctattgc tgttggagaa gatgggccaa ctcatgagcc aatagaacaa ttagcatcag
    19441 tgcgctcaat gcctaatttg aacgttatcc gtcccgcaga cggtaatgaa acaaacgctg
    19501 catggcaacg agctgtatca gagacagatc gaccaacgat gcttgtttta acacgtcaaa
    19561 acttaccagt tcttgaagga acatcagaat tagcccaaga aggggttaat aaaggtgctt
    19621 atattttatc agaagctaaa ggtgaacttg atggtatcat cattgcaaca ggttcagaag
    19681 ttaaattagc tttagatact caagacaaac ttgagtcaga gggcatccat gttcgcgtcg
    19741 tatctatgcc agcacaaaac atttttgatg aacaagaggc ttcatatcaa gagcaagtgc
    19801 ttccatcagc tgttacaaaa cgtcttgcta ttgaggcagg atcaagcttc ggttggggta
    19861 aatatgttgg tttaaatggg ctaacactta ctattgatac ttggggagca tcagcaccag
    19921 gtaacagaat tttcgaagag tatggcttta cagttgagaa tgctgtatcg ctatataaag
    19981 aactttaaga gcatgaagaa gttgggaaaa atacctcaac ttctttttgt aagatatagg
    20041 atggatttga gaatagtttt gatagactga taagtgatgg aaagcactct tttttgaaaa
    20101 cgtttgacaa acattccata gcgcagtata attgataaaa ctattgtata tataaaataa
    20161 tacaactatt attaattatc tttttagaaa ggtttgttta tgaagaagga gtgtcgtgat
    20221 ttttatcggc agatacagca tacttacaat gatatatctg tccgtgaaga tgcagtgcta
    20281 tcatcaatac tattatcagc atcgaatggt ttgataaaaa ctagcgatgt acctcgtgtt
    20341 gcttatgagt taactcagca attggaaaat aatgagatag aaaaatcttt cgagtcatta
    20401 gctacagtta aggaattaaa aaaatctgct aaaaaattgt ctgagagata tgagcaaaat
    20461 aaggtaacaa cgggattgct ttctaaagta tttggaaatc attaggagat aaaaggagat
    20521 ttttatgatt aagtttgaac atgtttcgaa agtatacggg gaaaaagaag ctctcagtga
    20581 tttaaccctc tctattaaag atggtgagat ttttggatta atcggtcaca atggagcagg
    20641 aaaaacaacg acaattagta ttctaacatc tattattgat gcaacttatg gccaagtcta
    20701 tattgatgac ctacttttga cagaacatcg tgaccaaatt aagaaaaaaa taggttatgt
    20761 accggactcg ccagatattt tcttgaattt aacagcagag gaatactggt actttttagc
    20821 taaaatttat gatgttgccc cagaagatat tgaagcacgt atcacaaaat tggtagacat
    20881 atttgaacta gaggagcagc gctataatcc tattgaaagt ttttcacatg gtatgcgtca
    20941 aaaagtcatt gttataggtg cccttcttcc aaatcctgac atttggattc tagatgagcc
    21001 attaactggt ttagatcctc aagcttcttt tgatttgaaa gaaatgatga aagaacatgc
    21061 taaaaatgga aaaacagtta ttttttcaac acatgtctta gcagttgcag aacagttatg
    21121 cgacagaata ggtattttga aacaagggaa attaatcttt gtcggtagct taggtgagtt
    21181 aaaaatgcaa tatccagata aagatttgga aactatctat ttggaattag caggacgtca
    21241 agctaatagg gagggataaa gtatgaattg gtcacgtatc tgggaactcg taaaaattaa
    21301 tatcctttat tcaaaccctc agactctatc ggcactaaga aaaaagcaag aaaagcatcc
    21361 taaaaaagaa ttttcagctt ataaatccat gtttagaaat cagttatttc agattttgct
    21421 cttttcaata atttatgtat ttctctttgt atcacttgat tttaaagaat atccgggcta
    21481 tttcacgttc tacattggta tctttacact agtatccatt atctactctt ttattgcgat
    21541 gtacagtgtt ttctatgaga gtgacgatgt taaacaatat gcttatttgc ctattaaatc
    21601 tgaagagctt tatgttgcta aaatttttgc aacatttggg atgtctgtaa cttttctaat
    21661 gccaatccta accttaatga ttgtggctta ttggcgtata ataggtggcc cacttgcggt
    21721 cctcctagca atcataaatt ttgctatttt atttttatcg gtaacggtga tttcattgta
    21781 tattaatagt ttaataggcc gtgccattat tcgaagtgct aatcggaaac ttatttctac
    21841 tatattaatt tcactagcta catttggagc gatagttccc ttattatttg tgaatatgac
    21901 cagtcaaaag atggtacaag ggaaattaca agatatcgct ccaattcctt atgtcagagg
    21961 ttattatgat attgttacag cgccgtttag tatggagagt cttctcaact attatctccc
    22021 gctcttaatc attttattct tgataggtgc tatttataaa tgggttatgc cacgctacta
    22081 tcaggagttg ttgtatggac aggtcaagca gagaaaagtc catcgtcaaa tcgattttag
    22141 taaacgtgag tcaattaata aaactcttgt taaacaccat ttatcaagtt tacaaaatgc
    22201 gacattattg acaaatacat ttttaatgcc actattatat ctagcaatgt tcattgttcc
    22261 aattttgaat aatggtaaag agattggtcg attttttaat gaaaattatt ttgggatagc
    22321 ttttctagca ggaatattaa taggtagttt atgtgtgatg ccagctagca ttgtaggggt
    22381 aggtatttct ttagaaaaaa gtaatttcta ctttataaaa agtttgccta tatcattttc
    22441 gtatttctta aagcataaat ttgtaacttt gataacatta caacttgcag ttcctacttt
    22501 tatttatttc ttggtaggat tctttctact taaactatca atcctcgttt tattgagctt
    22561 cattttaggt ctagtcttca tgggacttat cgaaggccag tttatttata gacgtgacta
    22621 taaacatttg ttcttgaatt ggcaagaggt tacgcaacta tttaatagag ggttaggcca
    22681 atggctgtta gttggttcat tgtttggaat gatgataata ggtagttttc ttattggaat
    22741 aagtattttt tggtcaatgg tatggaatac tgtagctgtc aatatcataa tccttatcat
    22801 tgggttgctc atattatcaa tttgccaata cttactgcta aaaaattttt ggaagaagct
    22861 tgtttagtaa aaagatttta gaaaaatcat acagtaagaa taatgcttaa taactgagat
    22921 agttatagcg gataaaataa aggcttgttg tggactttac ggttggaagg cagccactgt
    22981 aatcctttgt ttaataacgc tagttttagc tctaaaatat ttgattttta ttgctatcta
    23041 ttatatctta tcagacaaag agactaggga taacctagtc tctttgtctg atgctaactt
    23101 gtttttcgta atattgtgct agaatgaatg tcaatatagc agttgtgctt acagtaatga
    23161 taactgcaat aagtatggta ataaaaagat gtgttgaagt atgactgata taaagaaata
    23221 gacctggtag accagaagag cctacagcga agcggtgagc gttcattata ccggcataga
    23281 gtcctccaag accaccacca ataagtgagg ctattagagg ataacgtttc ggaaggttga
    23341 caccatataa gataggttcg gtgattccca tataagctgt tatggcagca ggtccagcaa
    23401 tttctttaag ctttttttct tttgttataa gagtcaccac tgtacctgca gttgcttgtg
    23461 cgatatttga gcagagcatg cctggtccaa aaatagaatc aacccctaat ttcgctagtt
    23521 gtgtgattcc taatgcagca attccactat gaacaccaaa catgattagg ataggagcaa
    23581 atgctcctac tagaaaagca ggtgcccaac tggcgtattt ggataaaaaa agtaaaaata
    23641 acagctaaat attggccagc gatagtaccc attggtccca atatagagaa cgacaaaatt
    23701 cccatgctga gaaatattaa cataggaaca agcactaaac gtagtgattt aggaataatt
    23761 tgatttaaat atttttctat ataagcctga acacaaataa ttagaataat gggaatgaca
    23821 cttgatgcat agttagtaag tgtgaatggg atcgtatgga ataaggatac tggtttacca
    23881 gaagctacta aattagccca attagggtga aggagcatga caacagttcc caaagctaaa
    23941 ataggattag ccttcaattt ttgagcagct gtgatggcga ttaagatagg taaaaaatag
    24001 aaaacaccat ctgcaaaaag atttagtaat atatatgttt gagataacgg tgtcaagatt
    24061 ttgaatacta gtaataaagc tagcaatgcc ttaatcatac cagcgcctga tagagctggt
    24121 aaaatcggtt ggaaagtccc agaaacaaaa tcaataattc gactaactgc tttggctttt
    24181 ttggtttgtt ctgttttagt cgttgagtta gtttcaatga gggagttaat ttcttcaaac
    24241 acttttgcga catgagtccc gatcactatc tgatgttgcc ctccgctgat aacgacagtt
    24301 tttacctctt ttaaagtttt taattgctca aggtttactt tcgtcggatc attgagacta
    24361 aagcgaaggc gtgtttgaca atggtaaaca ttgttaatat tattttgccc accaaggtgt
    24421 ttgatgatat tttttgacaa ggtgatgtat tctctagaca taatatctcc ttaatactta
    24481 taaaacgttt tccgattcta gattatagca tagtaataaa agaaaacact tggaaatgct
    24541 tgtgaatttt caatgatgtt ttcagatagc attcctaaag ggtctatgat ataatttttt
    24601 tatgaaaaga cattttgaga caactagaag aattgttatt aaagtaggaa ccagttccct
    24661 cgtacagaca agtgggaaaa ttaatttaag taaaatagac catttagcat ttgttatttc
    24721 tagtcttatg aataaaggta tggaggtgat tttagtatca tctggagcta tggggtttgg
    24781 tttagacatt ttgaaaatgg ataagcgtcc tcaagaaata tcacaacaac aagcagtttc
    24841 tagcgtagga caagtagcca tgatgagtct ttattctcag atattttcgc actatcaaac
    24901 tcatgtttcc caaattttat taacacgtga cgttgtggtt tttccagaga gtttgcaaaa
    24961 tgtaacaaac tcttttgaga gtctgctctc tatgggaatt ttgccaatcg tcaatgagaa
    25021 tgatgctgtc agcgtggatg agatggatca taaaacgaag tttggggaca atgaccgtct
    25081 ctctgctgtc gttgctaaaa ttactaaagc agatttatta attatgctgt cagatattga
    25141 tggtcttttt gataaaaatc ctaatattta tgatgatgct gttttacgta gtcatgtttc
    25201 tgagatcacc gatgacatca ttaagtcagc tggaggagca ggaagtaagt ttggtactgg
    25261 tggcatgctt agtaaaatta aaagtgcaca aatggtgttt gataacaatg gacaaatgat
    25321 attgatgaat ggcgccaatc ctcgtgatat tttaaaggtg ttagatgggc ataatattgg
    25381 aacttatttt gctcaaggaa agtgagattg agttatgacg tatattgaaa tattagggca
    25441 aaatgctaaa aaagctagtc aatcagtagc acgattaagt acagctagta aaaatgagat
    25501 actcagggac ttagcgagaa acatagtggc tgatactgaa accattttga ctgagaatgc
    25561 tcgcgatgtt gctaaggcaa aagataatgg tatctcagaa attatggtgg accgtctacg
    25621 actaaacaaa gaccgcatac aggcgatagc aaacggtatt tatcaggtag cagatttggc
    25681 agatccgatt ggtcaagttg ttagtggtta tacaaattta gatggtttaa aaatccttaa
    25741 aaaaagagtg cctttagggg ttattgcaat gatatttgaa agtcgtccca atgtatcggt
    25801 ggatgccttt agtttagctt ttaaaacagg gaacgctatt attttacgtg gtggtaaaga
    25861 tgctattttt tcaaatactg ccttggttaa ttgtatgcgc caaacattgc aagatacagg
    25921 ccacaaccca gatatagtac aattagttga agatactagt catgttgtag cagaggagtt
    25981 aatgcaggca acagactacg tggacgtctt aattccaaga ggtggtgcaa aattaattca
    26041 aacggtgaag gaaaagtcaa agattcctgt tatcgaaaca ggtgttggaa atgttcatat
    26101 ctatattgat gaatttgcag atttagatat ggcagctaag attgttatta atgctaaaac
    26161 ccaacgccct agtgtctgta atgctgctga agggttagtt gttcatcaag ctattgctaa
    26221 gggattttta tctcaattgg agaaaatgct caaagaatcg aaccagtcag ttgaatttcg
    26281 tgccgatgag gaagctttac aacttttaga aaatgctgtt gctgcaagtg agactgatta
    26341 tgctactgaa tttttagact atattatgtc agtaaaagta gtagattctt ttgaacaagc
    26401 aattagctgg attaataaat acagtagtca tcactcagaa gctatcatta ctaataatat
    26461 cagtagggca gaaatttttc aggatatggt agatgcagcg gcagtttatg ttaatgcttc
    26521 aacacgtttt acagatggtt tcgtttttgg actcggagca gagattggta tttcgactca
    26581 aaaattacac gcgcgtggac cgatgggatt agaagcttta acaagtacaa aatactatat
    26641 taatggaact ggtcaagtaa gggaatagtt gacctataat aggagagatt gcatttatcg
    26701 gtctctcttt tacttttcta aagtttagga tttatggtac aattaaagtt atgacaaacg
    26761 attttcatca tattactgtg cttttgcacg agactgtgga tatgcttgat attaagccgg
    26821 atggtaccta tgtagatgct actttaggtg gggcaggaca cagcgaatat cttctttcac
    26881 aattaggccc agatgggcac ctttacgcat ttgaccaaga ccagaaggct attgacattg
    26941 ctcatatacg tttaaagaaa tatgtggata ccggtcaagt aacctttatc aaagataatt
    27001 tcagaaatct atcttctaac ttaaaagcat taggagtgtc ggaaattaat ggtatttgtt
    27061 atgatttagg tgtatcaagc ccacagctgg acgagcgtga acgcggtttc tcatataaac
    27121 aagatgcccc ccttgatatg cgaatgaata gagagcagag ccttacagct tatgacgttg
    27181 tgaacactta tagttatcat gatttagtaa ggattttctt taagtatggc gaagataaat
    27241 tttcaaaaca aattgctcga aaaattgagc aagtacgagc tgaaaaacca atatctacaa
    27301 caacagaatt ggctgagatt atcaaatcgt ctaaatctgc caaagaactg aagaaaaaag
    27361 gtcacccagc taaacaaatt tttcaagcta ttcgtattga agtcaatgac gaactaggag
    27421 ctgcagatga atctatacaa caagcaatgg acttattagc tgttgatggt cgtatatctg
    27481 tcatcacttt tcattcttta gaagatcgtc ttactaagca actttttaaa gaagcatcaa
    27541 cagtagaagt acctaaaggt cttcctttta taccagatga tttacaacct aagatggagc
    27601 tcgttaatcg taaacccatc ttaccaagtc aagaagaact agaagcgaac aatcgtgccc
    27661 attcagcgaa attgagagta gcgcgtcgta ttcgataata agaaagaaac ttatgacaaa
    27721 tgaaaaacga actgaagcag taacacaaac cttacaacgt cacatcaaga ctttctctcg
    27781 cattgagaaa gctttttatg gcgctattgt aattactgct attatcatgg cagtcggtat
    27841 tatttacttg caaagtaata gtttacaagt taaacaagaa gtaaatcaat taaatagtaa
    27901 aatcaacgat aaacagacag aatttgacaa tgctaaacaa gaagtaaatg agctttctaa
    27961 tcgtgatcga attactaaaa ttgctaaaga tgctggttta acaattcaaa acgacaatat
    28021 ttatcggaag gtggactagt ctgtgacttt ttttaaaaag ctaaaaaaaa tcttccttga
    28081 ttatgtgatc catataagag ataggcgttc gccgcagaaa aatagggaaa gagtaggtca
    28141 aaatctgatg attttaacca ttttcctatt tttcattttt atcatcaact ttgtcatcat
    28201 cgttggtaca gatagtaaat ttggggttaa tttatccaag gaagccaaga aagtctatca
    28261 acaaagtatg acagtacagg cgaagcgtgg gacaatttac gaccgtaatg gaaatcctat
    28321 cgctgaggat gctacgacct atagtctcta cgctattatc tcaaagaact atacgacagc
    28381 tacaggtcaa aaactatatg tacaaccttc tcagtatgaa aaagtagcta gtatcttaga
    28441 gaataaattg ggcatgaaga aaaatcttgt cctaaaacaa ctcaatcaaa agaaattgtt
    28501 tcaagtttcc tttggaagtt caggttcagg attgtcttat accaagatgg ctgacatcaa
    28561 gaaaacgatg gaaaagtcag atatcaaagg aattggtttt tctaccagtc cggggcgtat
    28621 ttatccaaat gggatatttg cttcacaatt tataggattt actcttcctc aggatgatgg
    28681 agatggaaaa aaattagttg gtaatacagg ccttgaagcg gcacttaaca aggtattatc
    28741 aggtactgat ggcaaagtaa catacgaaaa agaccgtagt gggaatgttc ttttaggtac
    28801 tgcaacaaca gaacgtaggg cggttaatgg taaggatatc tacacaactt tatcagagcc
    28861 tattcagact gttttagaga ctcagatgga tgttttcgca gaaaaaacca aaggaaaatt
    28921 tgcgagtgct acagttgtca acgctaaaac gggagaaatt ctagcaacga gtcagcgtcc
    28981 tacttataat ccttctaccc ttaaaggtta cgataagaaa aatttgggta cctataacac
    29041 actactatat gataattttt ttgaacctgg ttcaacaatg aaggtaatga ctctagcctc
    29101 tgcaattgat tctaagcatt ttaactctac tgaagtgtat aacagtgcac aatataaaat
    29161 tgcagatgcg gttattcgag attgggatgt caatgaaggg ttatcatctg gtagttatat
    29221 gacttttcca caagggtttg cacattccag taacgttggt atggtaacct tggaacaaaa
    29281 aatgggacga gataaatggt taaactatct ttctaagttt aaatttggtt acccaacacg
    29341 ttttgggatg cttcatgaaa gtggaggttt gttcccatct gacaatgaag taactatcgc
    29401 tatgagctca tttggtcaag gtattggtgt aacgcaagtt caaatgttgc gcgcatttac
    29461 gtctatctcg aatgatggtg ttatgttaca accacagttt attagcagta tttatgatcc
    29521 aaatacaggg acttctcgta cagcacgaaa agaagtcgtt ggaaaaccag tttccaaaga
    29581 agcagctagt aaaacacgtg attatatggt tactgttggg acagatcctt attatggtac
    29641 attatacgca gcaggtgccc cagttattca ggtggggaat caatcagttg cagtaaaatc
    29701 tggtactgcc caaatcgccc aggaaggtgg tggtggttat ctgcaaggaa aaaatgatac
    29761 tattaactca gttgtggcta tggtgccatc ggaaaatcct gacttcatca tgtatgttac
    29821 catccaacaa cccgaaaaat ttagtatcac attctggaaa gatgttgtta atcctgtttt
    29881 agaacaagca acagcaatga aagaaacgat attaaaaccg gtattgaatg actcagaaca
    29941 tcagacaaaa tataaactat caaaaatagt tggtgaaaat ccaggtcatg tagcggaaga
    30001 attacgtcgt aatctggttc agccaattat tttaggaaat ggtagtaaag tttctaaagt
    30061 atctaaaaga ccaggagcta atcttgctga gaatgaacag ctactagtat taacaaataa
    30121 actgactgaa ttacctgata tgtatggttg gtctaaagca aacgttgaac agtttgctaa
    30181 atggacaggt ataaaagtga cctataaagg ttcaacctca ggtaaagtaa gaaaacaaag
    30241 tattgatgtt ggaaaatcca tcaataaaat taaaaaaatc aaaattacaa taggagatta
    30301 acatgttttt aagtataatg gcaggtgtca tagcatttgt cctgacagtt attgccattc
    30361 cacgcttcat taagttttac caattgaaga aaattggcgg gcaacaaatg catgaagatg
    30421 tcaaacaaca tctagccaaa gcaggtacgc cgacaatggg aggaacggta tttttggttg
    30481 ttgctcttct tgtctcactt atttttagta ttattttatc taaagaaaat agtggtaatt
    30541 taggtgcaac ttttggtatt ttatctgttg ttctcatcta tggaattatt ggttttttag
    30601 atgatttttt gaaaattttt aaacaaatca atgagggctt gactccaaag caaaaaatgt
    30661 cgctccaact aattgcgggt ctaatttttt attttgtcca tgtgttacca agtggcacat
    30721 ctgcgataaa tatttttggt tttaaccttg aagtaggata cttatacgct ttttttgttc
    30781 ttttctgggt tgttggtttt tcaaatgctg tgaacctaac agatggaatt gatggtctag
    30841 cttctatttc ggttgttatc agtttgatta cttatggaat cattgcttat aatcaaacgc
    30901 aatttgatat tttattgatt atcgttatca tgattggtgc tttacttggc ttttttgtct
    30961 ttaatcataa accagctaaa gtctttatgg gagatgttgg ttcacttgca ttgggtgcta
    31021 tgttagcagc catttcaatt gccttacgtc aggaatggac cttgcttttt atcggctttg
    31081 tctatgtttt tgagacgtct tcagtgatgt tgcaagtagc atactttaag tatactaaga
    31141 agaaaactgg tgttgggaaa cgtatcttcc gcatgacacc atttcatcac catttagaat
    31201 taggtggtgt gtctggcaaa ggtaataaat ggagcgaatg gaaagtagat gcttttttat
    31261 gggctattgg tattttcatg agcgctatta cacttgctat tttatatctt taaactttat
    31321 taggggatgt ggtatggact gccagattcc cttttgcttt cttgtagcct ttaatggtat
    31381 aatgaacata ataatagtaa ggaaatagac atgtcattta aagattttaa ttttaaacca
    31441 tacattcaga gggctctcga tgagttaaaa ttcgtcgatc caactgatgt tcaagctaaa
    31501 ttgattccag ttgttcgttc aggccgcgat cttgtggggg aatcgaaaac tggttctggt
    31561 aaaacccaca cctttttact tcctattttt gagaagctag atgaaagtag tgatgatgtt
    31621 caagttgtta ttactgcacc aagtagagag ttggcaacac aaatttatca ggcaactaaa
    31681 caaattgctg agcattcaga gcaagaaata cgggtggtta actatgttgg tggtactgac
    31741 aaattacgtc aaattgaaaa gttgaaagta agccaacctc atattgtcat tggaacccca
    31801 ggacgtattt atgatttggt gaagtcaggt gatttagtta ttcataaggc gcatactttt
    31861 gtggttgatg aagcagatat gactctggat atgggattcc ttgataccgt tgataagatt
    31921 gcagggtctt tacccaagga tgttcaaatt ttagtctttt cagcaacgat tccacaaaaa
    31981 ttacagccat ttttaaagaa atatttgacc aatccagtaa tggaaaaaat caaaacagca
    32041 acagttatcg ctgataccat cgataactgg cttttgtcca ctaaaggacg tgataaaaat
    32101 gcacaaattt tggaactctc taaactaatg cagccttacc ttgcaatgat atttgttaat
    32161 acgaaagagc gtgcagacga attacactcg tatctttcta gtaatggctt aaaagtagct
    32221 aaaattcatg gtgggattgc acctcgtgaa cgtaaacgta ttatgaatca ggttaaaaac
    32281 ctcgaatttg aatatatcgt tgcaacagat ttagcagcgc gtggtattga tattgaaggt
    32341 gttagccatg ttatcaatga tgccatccct caagacctat ctttctttgt ccaccgtgtc
    32401 ggtcgcacag gccgtaatgg cttatctggt actgctataa ctttatatca gccaagtgac
    32461 gattctgata ttcgtgaact tgaaaaacta ggtattaatt ttattcctaa agtcattaaa
    32521 aacggtgaat ttcaagatac atacgaccgt gatcgtcgta ataaccgtga aaagtcctat
    32581 caaaaattag atacagaaat gattggactt gtgaaaaaga aaaagaaaaa aataaaacca
    32641 ggttacaaga aaaaaattca atggaaagtt gaagaaaagc gtcgcaaaga gcgtagagca
    32701 agtaatcgtg ctaaggggcg tgcggagcgc aaagctaaaa aacaaagttt ttaaaattat
    32761 taaagtctga aatcagttcg tttcaggctt ttttattgat ataaaatctt tatcacctga
    32821 taaggaaata ctattataca gatagatttt agagtgttag actagtaata tgatatttta
    32881 ggagaattct tatgtttaaa aaaataatat tatctattgc aactatagcc gcaaccgcca
    32941 gcctagcagt atcagttcag gcgtcagaga aagtagaact taaagtagct acagattctg
    33001 acacggcacc atttacttat caaaaagacg ggaaattcaa aggttatgac gttgatgttg
    33061 tcaaagctgt ttttaaaggt agtaagtaca aagtaacctt caagacagtt ccttttgata
    33121 ctatttcaac aggtattgat gcagggaaat ttgatttatc agctaatgat ttttcatata
    33181 ataaagaaag agcagaaaaa tatctcttct cagatcctat atcccgttca aattatgccg
    33241 tagtagggaa gaaggggagc cattacaaat cattaagtga cctctctgga aaatcaacag
    33301 aagttttatc tggcgttaac tatgcacagg ttctagaaaa ttggaataaa aatcatccta
    33361 ataaaaaacc aataaaaatc aaatatgttt ctgggacaac tggtgttact agcagattaa
    33421 aaaatattga gagtggaaaa attgacttta tcctatatga tgccatttca tctgactata
    33481 ttgtaaaaga tcaatcatta aacttaagcg tttctccttt gaaaggtaaa attggtaata
    33541 ataaggatgg attagaatac ctccttttac caaaagataa aaaaggtaaa actctacaga
    33601 aatttataaa taagcgtatt aaagttttga aagaagatgg tactttggca cgtttaagta
    33661 aacaatattt cggtggagat tacgtttcaa acattgataa ataaatcagt gagactataa
    33721 gttttatctt tttacatgtg agagtttgcg tgatagaata acgaagataa gaaagtttag
    33781 aaaaggaaag tagaaggaat gaatcaattt atcttgacgg gtggatggtc ttggtataat
    33841 aatttggtta gtcaggttcc agcggggaaa ttatttagtt ggaaggctgt tttagatgca
    33901 ataccctcaa ttttagagcg attacccata acgctattat tgacagtagc aggagcctta
    33961 tttggactca ttttagcttt gatttttgca gtagtgaaaa ttaaccgtgt caaaatttta
    34021 tatccgattc aagctttatt tgttagtttt ttacgtggga ctccaatact tgttcaatta
    34081 atgctgagtt attatggaat tccacttttc ttgaaatttt taaaccaaaa atatggtttt
    34141 gattggaaca ttaatgctat accggcttct gtatttgcta ttactgcatt tgcttttaat
    34201 gaagcggctt atacaagtga aactatacga gcggcgattt tgtcggttga tcaaggtgaa
    34261 attgaggcag cacgtagttt agggatgacc tcagcgcaag tttatcgtcg tgttattatt
    34321 ccaaacgcag cagtagtcgc aacacctact ttgataaaca cgttaattgg tctaactaag
    34381 ggaacctctt tagcttttaa tgctggtatt gttgaaatgt ttgcccaagc acaaattatg
    34441 ggtggtagcg attatcgcta ttttgaacgt tatatctctg tggcacttgt ttactgggcg
    34501 gttagtttct taattgaaca attgggaaat gctattgaac gaaaaatggc aattaaggct
    34561 ccacaccatt taactgatga gattccagga ggagtacgct aatgataaaa ttgagacaat
    34621 tgactaaatc attttctggt caaaaagtgt tagacaaact tgatttagat attgaaaaag
    34681 gtcaagttgt tgctctagta ggtgcttcag gtgcaggaaa atcaaccttt cttcgtagta
    34741 tgaattattt agaagagcct gattatggaa caattgaaat tgatgatttt aaggttgatt
    34801 ttaaatcaat cagtaaagat gacatactaa cattacgccg taagttagcc atggtttttc
    34861 aacaattcaa tctatttgag cgtcgtacag ctttggataa tgtaaaagaa ggtttgaaaa
    34921 ttgtcaaaaa gatgtcagat caagaggcaa cacggattgc aagagacgaa ctggcaaagg
    34981 tcggacttgc agatcgtgaa aaatattatc caagacattt atcaggtgga cagaaacaac
    35041 gtgttgcttt ggcacgcgca ttagccatga aacctgatgt tcttctttta gacgaaccga
    35101 catcagcact tgatccagaa ttagttggag aagttgaaaa atctattgca gatgctgcta
    35161 agcaggggca aaccatggtg cttgttagtc atgatatgaa ttttgtttac caagtagcag
    35221 ataaagtttt atttttggaa aaaggtcgta ttttagagtc tgggacgcct gaacaattat
    35281 ttaaccatcc tttagaagaa cgtacaaaag aattcttcgc tagttataac aaatcgtacc
    35341 tataagctaa aatattttga tataatgaag gttagaatgt ctaatcttca tttttaatta
    35401 ttcttgagtt aggctaagta tataagaaag ctgcgatatg ttttgacaat cgcttgttgg
    35461 atagagatgt ttttagaaat agcaaaagtt tatttacaag gtttagggct tgccagcata
    35521 actgttattt tagttggtat attttgggtc ttttggcgtg ccaagaaaaa catagataaa
    35581 acagctagag aacgccaatc tgttctttat gatgtccttc tgatagctct tatgacagct
    35641 ccaatacttg cctttgcatt tgtagctatc attttaatga taaaagctta gtcagattag
    35701 ttggaaatat agacgctttg ctatagaata aaaccataag attcaaagga gaaatagtaa
    35761 tgtatgatac tttaataata ggttcaggtc ctggtggtat gacagcagcg ctttacgctg
    35821 cacgttcaaa ccttaaagtt gggttaattg aacaaggtgc tccaggtgga cagatgaata
    35881 atactgctga aattgagaac tatccaggct acgatcatat ctcaggtcca gaattatcaa
    35941 tgaagatgta tgagccgttg gaaaaatttg aagtagagca tatttatggt atagttcaaa
    36001 gagttgagaa cgatggtgat gtaaagcgtg ttatcaccga agatgaatcg tatgaagcca
    36061 aaacagtaat ccttgctacc ggagctaaaa acagcctttt aggagttccg ggtgaagagg
    36121 aatatactag tcgtggcgtt tcttactgtg cggtttgtga tggtgcattt ttccgtgatc
    36181 aggatctttt agttgtgggt ggcggtgatt ctgctgttga agaagctgtc ttcttaactc
    36241 aatttgctaa aagtgttact attattcacc gacgtgatca attgagagct caaaaagtac
    36301 ttcaagaccg agcttttgct aacgaaaaaa taaaatttgt atgggattca gtggtcaaag
    36361 agattaaagg aaatgagatt aaagtatctg gtgtaactgt tgaaaatcta aagactggag
    36421 aaatctcaga gatgactttc ggaggtgttt tcatttacgt tggtttgaaa ccacattcta
    36481 gcatggtctc agaattaggt attacggacg agactggctg ggtcttaact gatacaaata
    36541 tgaaaacatc aattccagga ctatatgcta ttggggatgt tcgtcaaaaa gatctacgtc
    36601 agattgcgac tgctgttggt gaaggtgcta tcgctggaca gggtgtttat aactatataa
    36661 ctgagcattt ctaagaaata ctaaaagtag aaaagactat cgaaaaacga ttagtctttc
    36721 tttttatttt catgaaagta aggtgataaa ctgaactacc gagatagaaa tatgctataa
    36781 tgttagacat atagccttat cctaggctca tgattaaaag gagattgtct gatgtataaa
    36841 gacgatagtt taacactaca tactgattta tatcaaatta atatgatgca agtctatttt
    36901 aataaaggta ttcacaataa aaaagcggtg tttgaagctt actttcgcaa ggttccattt
    36961 gaaaatggtt atgctgtttt tgcaggttta gaacgtattg ttcgttattt agaaaatctg
    37021 agcttttctg atagtgattt atcctattta gaagagttag gttatcctga agaattttta
    37081 gattacttaa aaaatttaaa aatggaactg actgtcaaat cagctaagga aggtgacctt
    37141 gtatttgcta atgaaccatt agttcaaatt gaaggtcctt tagcacaatg tcagttagta
    37201 gagacagcga ttttaaatat catcaactac cagacacttg ttgctacaaa agcagctcgt
    37261 attcgttctg ttattgaaga tgaaccgcta ttagagtttg ggacacgacg tgctcaagaa
    37321 atggatgcag ccatttgggg tactagggca gcgattattg gtggtgctaa cgcaacgagt
    37381 aatgttcgtg ctggtaaaat ctttaatatt ccagtatcag gaacgcatgc acatgcttta
    37441 gttcaaactt atggcgatga ttaccaggca tttaaagctt acgcagaaac tcataaagat
    37501 tgcgtctttt tagtggatac ttatgatacc cttcgtgtag gtgtgcctaa tgctattcga
    37561 gttgcaaaag agatgggcga aaagattaat ttcttgggag ttcgccttga ttcaggtgat
    37621 ttagcttatt tgtctaagaa agttcgtcag cagttagatg acgccggttt tcctaatgca
    37681 aagatttatg cttcgaatga tctagatgaa aatacaatcc tcaacctaaa aatgcaaaaa
    37741 gctaaaattg atgtttgggg tgtcggaaca aaattaatta cagcctatga tcagcctgcg
    37801 ctaggtgctg tttataaaat tgtttctatt gagactgacg ccggtagtat gcgagataca
    37861 attaaactat caaacaatgc ggaaaaagta tcaacacctg ggaaaaagca agtttggcgt
    37921 atcaccagtc gagctaaagg gaaatctgag ggtgattaca tcacatttgc agacactgat
    37981 gtgacacaat tagatgaaat tgacatgttt catccaactt atacttatat caataaaaca
    38041 gtccgtgatt ttgacgcagt acccttactg gtagatatct ttgacaaagg gaaattagtt
    38101 tatcaattac caagccttca agaaattcaa gagtacggac gcaaagaatt tgatcagctt
    38161 tgggatgagt ataaacgtgt tctgaatcca caggattacc cagttgattt ggcacgcgat
    38221 gtttggcaaa ataaaatgga cctgattgat cgtatccgta aagaagcttt ggcaaaagga
    38281 gaagtaagat gactttgcaa gatcaaatta tcaaagaact cggtgtaaaa cctgttatta
    38341 atccaagtca agaaatccgt cgttccgtag aatttttaaa agactatcta ttaaaacata
    38401 gttttctaaa gacctatgta ctgggtattt caggggggca agattcaact ttagcaggac
    38461 gtctagctca actagcagtt gaagagttgc gtgctgatac aggggaaaac tatcaattta
    38521 tagctattcg tttaccatat ggtattcagg ccgatgaaga agatgcacaa aaagcattag
    38581 attttatcaa gccagatatt gctttgacca ttaatattaa agaagctgtt gatggccaag
    38641 ttagagctct taatgcagca ggagtagaga ttacagactt taataaagga aatattaaag
    38701 ctcgtcaaag aatgattagt caatatgcag ttgcaggtca atatgctgga gcggttattg
    38761 gtactgatca tgctgcagaa aatattacag gttttttcac caaatttggt gatggaggtg
    38821 cggacttatt acctctcttt agacttaata aaagtcaggg aaaacagtta ttagctgaac
    38881 ttggtgctga taaagacttg tacgaaaaga ttccaaccgc agatttagaa gaaaataaac
    38941 caggaatcgc tgatgaaata gcacttggag taacctacca agaaattgat gcttatcttg
    39001 aagggaaggt agtttcggac aagtctcgag gtatcattga aaattggtgg tataagggtc
    39061 aacataaacg tcatcttcca atcacaattt ttgatgattt ctggaaataa gaaaaatagc
    39121 tagaatattc tagctatttt tgtttctcta ataaattact ttcagacttg cagaaatatt
    39181 cagaatgttt ataatagtag ctaagtataa aaggaggaat ttatgtcaga attaactcaa
    39241 acatttacag ataagttatt agctgattac caagcaaata caaaatttag cgctattgaa
    39301 aatgcagtaa ctcacaatgg tttattgaag tctctagaga ctcgtcaaag tgagattgag
    39361 aacgactatg tcttttcaat tgatttgacc aaagatgaag tctctaatca gaaacaatca
    39421 ggacgttgct ggatgtttgc tgcactgaac acatttcgtc ataaactgat ttcagatttt
    39481 aaactcgaga actttgaatt atctcaggca catacatttt tctgggataa gtatgaaaaa
    39541 tcaaattggt ttatggaaca aattattgcg actgcaaatc aggaattgag tagtcgtaag
    39601 gtaaaattcc tattagatgt acctcaacaa gatggcggac aatgggacat ggtggttgct
    39661 ctgtttgaga aatatggtgt cgttcctaaa acagtttatc ctgaatctgt ttcatcaagt
    39721 gctagtcgtg aactaaatca atatttgaat aaacttttac gccaagatgc acagattcta
    39781 cgagaattaa tagcgcaggg agcagatggg gcaactgttc aaaataaaaa ggaagaattg
    39841 cttcaagaaa tttttaactt tttagcaatg aaccttggct tgccaccgca atcttttgat
    39901 tttgcttatc gtgataaaga caatcattat cagtctgata aaaatattac accgaaagca
    39961 ttttatcaaa aatatgttaa tcttgacata tcagattatg tttctattat caatgctcca
    40021 actgtagata agccttatgg acaatcctat acagttgaga tgcttggaaa tgttgtcgga
    40081 ggcccagctg ttaaatattt aaaccttgat atgaaacggt ttaaagagtt agcgattgcg
    40141 caaatgaaat caggagaaac tgtgtggttt ggttcagacg taggtcaagt atcaaatcgt
    40201 caaaagggta tcctagcaac tacgacttat gattttaatt catctatgga tattaaactt
    40261 agccaagaca aagcgggccg cctagattat agtgaaagtt taatgacaca tgctatggtc
    40321 ttaacgggag tagaccttga tgaatctgga caacctttaa aatggaaagt tgagaactct
    40381 tggggagaga aagttggtaa ggatggttat tttgtagctt cagatgcttg gatggatgaa
    40441 tatacttacc aaatagtcgt tcgtaaagaa ttgcttacta aagaagaatt agaggcttac
    40501 aacgcagagc caattacttt agcgccgtgg gatccgatgg gagcactagc taattagtaa
    40561 tagtaattat aaattaaaaa actggatata ttatccagtt tttttaatta ccgttaggtg
    40621 ctgtattgtt attaccatta ccatgtccag ttcctggcac agtttgattg gaattattag
    40681 gggtagtagc attaccatta ttaggtgttt cgacttgtgg ggatgatgaa ttggcatcat
    40741 ctgctgtatt cttatcctct ttttgtttct cagaggatgt tgaactactg ctttgacttg
    40801 actgtccaga attttgatag atattcttat agacagaaga agagtaagtg ccggttgtag
    40861 ttgtcccatt gatatacaag tagcttccac ttcgatagag cccttctggc attgtccaat
    40921 ctgcactgta tccgccagtt aggtaggaca tcattgcacg ataaacctca gtagcaatat
    40981 ccagttggct accataaagt ggtgtaaggc gatttttata accagtccaa attgccattg
    41041 tgtacttaga agtatagccg acaaagtttt cgtcaggagc cattgtacca acggcgctat
    41101 tgtagatacc agtagttgct tcaattttag ctaactcatc ttctgtatag ttggaagtac
    41161 cagtcttacc agcttgtgca acaccaggga tagctgcttt agtaccagta ccaaatgtta
    41221 gtactgtttt caacatatcc gtcatcatgt aggcagtagt ctctttcatc gcacggctac
    41281 cagacgctgc ataagtatca ttggttccat cgctaaattc aattttatta acatattgcg
    41341 gcttatagta agttccgccg tttgcaaaag ccgagtaagc cgctgccatt ttctcactac
    41401 ttgcaccata ttttgcatca ctgctattgt tgttacttga aatagcattt gaatagttca
    41461 tttctggata gtatatgcct aatttttcaa ggaaagattt tgcttcgtct aatccagcgg
    41521 cttcaagtgc tctgacagca gggacgttac gtgattgttg aatagcggtc tgcatactca
    41581 tccaacccat atattgacga tcccagtcat atagttgagt agaagtacca ggccagtagt
    41641 aaactgagtc gtttaatgat tgacctgttg aattatagac accactatca atagcaggtg
    41701 cataagctga gataggtttc attgtagaac cccaatcgcg gtctgttaag acagattgat
    41761 ttgtcccaaa tgaaatattt tcattctgat gacgcccgcc taattgtgca atgactttac
    41821 cattagtcgc atccatgatg gtagatgcta tttgtaattc attgtttgga taagcgatgt
    41881 aagtatcact gttgtagatg tcatatagtt gtttttgtgc atcagtgttg atattagtat
    41941 acacttttag cccagcagta aagatatctt taccagtttt ttgtttaact tcactaataa
    42001 cttgttttaa gtagttatcc atatattttg gataagtaga tttttgcttt aattctttta
    42061 agccatcagt tactggagtt gcaacagctt ggtcgtattc ctttttagaa atgtttttat
    42121 cttgatacat ctgctgaaga acggtattac gtcttgtttg agcagattct gggtttttat
    42181 aagggtcata ttgtgtaggt gcttgaggaa taccagcgag caaagcaagt tgtgcaatag
    42241 ataattcctt taggtcttta ccaaagtatg atttagctgt tgttctcata ccataattcc
    42301 catttcccat ataaacttta ttaatataga aagtaagaat ttcttctttg gtgtatttac
    42361 gctccatttg aagcgcaagc caaacttcct gtgatttacg ttttaacgtt tggtcagatt
    42421 tattggtaga aaagtaagcc agtttaatca actgttgatc aagggttgaa ccaccttgcg
    42481 tattactact aacaaggtta tgccaagctg cacctaaaat acgataaata tcgacacctc
    42541 tatgtttaaa gaaacgttta tcttctatag aagtgatagc gttaaccaaa tttagtggaa
    42601 tgctatccgc actaacactt tcacgctttt ctgagcctaa atcggcaata agtttattat
    42661 taccatcata aaccaaacta gagttaacgg attttaaagc ttgatcggtt aatttcggag
    42721 aacgactaac gtagtatgca aataggagcc cacctattac aatagctaag ataataaatc
    42781 ccattataat gccaaaagca tacttcaata gtttgataac agattccttt ttaattgtaa
    42841 tcaccgccta ataatttttc ttcaataata tctaagtaag ggacttgagg aaaggcactc
    42901 tcctttacga aaaatccatt ttttctgata taatcaatag gcattgattt attgccttta
    42961 tcaatctgat aaaatgaaat taactcatta gcagggagta gataggtttc cttaagtgtg
    43021 gaaaaatgaa gcaagacaaa gcaaatccct ttttgctgta atacatttgc catgtgctct
    43081 atttggtgag catgaaaatt tttcataggc atagcagttt tctgccgggt ttctttggct
    43141 tcaaagtcaa tatagtaacc tttgtaaaca ccagaataat cagtagtaga agcttgtcta
    43201 aaataagctt ctacaatctt ggcacgacta cgtttaggat aatctacctt gacgatttgt
    43261 actggcgttg gtttcttatg aatgacagct aattcgtgag acaaatagta gtcattggtt
    43321 gcattgatag cagcttcaaa tgacatacca cggttggcaa aatcgatttt ctttttcttt
    43381 gattttgtaa ctgttgtttt ccgaataagc tgatgtggat agttaaccat ttttctcctt
    43441 gcattacaaa taaaatgcta agataagcaa aactattata ccataaaatt aggatggaca
    43501 acaaatgaag tcaactattc ttgtaacagg ttacaaaaat tttgaattag ggattttcca
    43561 ggataaggat cctcggatta ctattataaa aaaggctatt gataaggatt ttagacgctt
    43621 cttggaaaat ggagcagact ggtttatttt tatgggaaat ttagggtttg agtactgggc
    43681 tttggaagtt gctcttgatt tacaaaaaga atatgatttt caaattgcga caatttttac
    43741 ctttgagaac catggccaaa attggaatga ggctaataaa gctaagttgg cactctttaa
    43801 gcaagttgac tttgttaaat atactttccc aagctatgaa aacccgggtc aatttaaaca
    43861 atacaatcat tttctcatta acaacacaca aggtgcatat cttttttatg attctgaaaa
    43921 tgaaactaat ttaaaatttt tgctagaaat gatggaaaaa aaggaagctt atgatatttc
    43981 tttcctaact tttgatcgct taaatgaaat atatgaggaa tgatcgtttt ttcggatatg
    44041 agaacgtttt acgcttgatt tttccattta atttaggtat tatttacttt gatataaagg
    44101 ataggactta tattaaaacg atttgactgg tttggagaat agaatggcaa gtattattta
    44161 tagcccaaaa gatatttttg agcaagattt taaagttagt atgcggggat atgacaagaa
    44221 agaagtcgat gtatttctgg atgatgtcat caaagattac gaaaattatc ttgagcaaat
    44281 tgagaagctc caaatggaaa atagacgtct acaacaagct ttagacaaaa aagaaagcga
    44341 agcttcaaat gtaagaaatt cagggacagc tatgtataat caaaaaccga ttgcccaatc
    44401 tgcaaccaat tttgatattt taaaacgcat tagccgcctt gagaaagaag tttttggaag
    44461 acaaattaga gaataaggat ttctcaaata tgcaattttt ggataatcgc gtagtattga
    44521 tatactatga ggaaagtcca tgctagcact ggctgtgatg ccagtagtgt ttgtgctagg
    44581 cgaaaaaata agcctaggga gatagctagc tatcttacgg caagcaaaag ggctaagtct
    44641 ttggatatgc ctgaatagct ttgaaagtgc cacagtgacg tagtttctag ggaaatctag
    44701 aaagtggaac gcggtaaacc cctcaagcta gcaacccaaa ctttggtagg ggcatgggat
    44761 agttggaatg agaacaatct atcctgactg ctttgcagta gacagatgat tatcgaaaga
    44821 aacaattcct agttgtttct ggaacaaaac atggcttata gaaaattgca taataggtta
    44881 gtggctgaga taacatctca gccaattttt aactgtgatg aatacgatag tgtaaaagag
    44941 gaaaaatgaa agaatcattc aaattaatag caactgctgc tgctggttta gaagccattg
    45001 ttggtagaga aatcagaaac ctaggaattg actgccaagt tgagaacggt agagtccgtt
    45061 tccacggaga catcaaaaca atcattgaaa cgaacctatg gctacgagct gccgatcgta
    45121 taaagattat tgtaggagag tttccagcac gtacttttga ggaacttttt cagggtgtat
    45181 atggccttga ctgggaaaat tatttaccgt taggggcaaa atttcctatt gctaaagcaa
    45241 agtgtgtcaa gtcaaaattg cacaatgaac ctagtgtaca agccatttct aaaaaagctg
    45301 ttgctaagaa acttcaaaaa gtctttcacc gtcctgaagg agttccctta caagaaaatg
    45361 gagctgaatt taaaatagaa gtttctattt taaaagataa agcgactgtc atgattgata
    45421 cgactggatc aagtctcttc aaacgtggtt accgagcaga aaaaggtggt gcgccaatta
    45481 aagaaaacat ggcagcagct attattcaat tatctaattg gttccctgat aagccattga
    45541 ttgatccaac ttgtggttca ggtacttttt gtattgaagc cgctatgatt ggaatgaata
    45601 tagctcctgg ttttaatcgt gattttgcct ttgaggcgtg gccatgggta gatcaaagtc
    45661 aagtacaaaa ggttcgcgat gaagctgaga gtaaagcaaa ctatgatatt gatttagata
    45721 tttcaggttt tgatttagac ggtcgtatgg tagaaattgc tagaaaaaat gcagaagaag
    45781 caggacttgg agatgttatc aaattgaaac aaatgcgtct tcaagattta aaaacagata
    45841 aaattaacgg tgttattatc tccaacccac catatggtga acgactcctg gatgacaaag
    45901 cagtggacat tttgtataat gagatgggac aaacatttgc gcctttaaaa acttggagca
    45961 agtttatcct aactagtgac gaaggatttg aaaaaaaata cggctcacaa gctgataaaa
    46021 aacgtaagct ttacaatggt actttaaaag ttgatttgta ccaatattac ggtgaacgtg
    46081 tgagacgtca agtaaaataa cggggagaac tcatgtcaga agatcaaaaa cacccatttt
    46141 ttgaaccaga aaaagaaact gatggtcttg aatttaaaga tgctaaagaa atgacagtag
    46201 aagaggctgt tagaaaagat tctgaaatta aggctggtat taccgaagaa gatagtattc
    46261 ttgataaata catcaaacaa catcgtgatg aagtagcttc acaaaagttt gagacaaaat
    46321 caagcgattt tgcaaacctt gatacggcat ctttagatga ttttatcaag aaacagcgcg
    46381 aagaattatc agcgatgtta gctgcagaag agctttctaa aaagttggat aattcagtct
    46441 ctcaagaaca agatatagaa gctaatgctg tctcacctaa agaagaatct tcacaagaac
    46501 aagaaaatag tgtaactcca gttcctcctc tgaatacgga agcagaacca actgcaacag
    46561 aaccagattc gactattgct gattcagagg agtataaatc ctcttctaag aagcgtggag
    46621 gtattgtagg aacattgatt gctcttattt tacttctaat agtaacaatc tttggttaca
    46681 actatttcaa gaataataat tcgacaaatt ctcaaactgc aacaagtcaa agttctagtt
    46741 ctaaggctac gacgacttca tcagaagaag ataaaaaagc tagtcagaac ttagataatt
    46801 tcaacaaagc ttatgctaac ttttttgttg atgataagaa aacccaactc aaaaatagcg
    46861 aatttgataa attgtcagaa ttagaaaaga aagttgatgc tttaaaagga actaaatact
    46921 acggtaaagt aaaagttaaa tttgatagtc taaaacgtca aattgatgct gttaaagctg
    46981 ttaacgacaa gtttaagtcg cctgctgtag ttgatggtaa gaagagtgaa aaattagaag
    47041 taaaagatgg agcgaatttt gactctttag atagtaaaac tcttaatact ggtaatgcta
    47101 gtctagatag tttacttcac tccatcgttt caactggtcg taatcaggta aaacaatccg
    47161 aagaacaggc taaaaaaact caagaagaac agtcttcgtc taataaggtt tcagacactc
    47221 aaacaactga acaaccaaac gtaactaacg gtcaatcttc ctcatcagct gctacaataa
    47281 ataatcaggc agctggcact gcatcaggta atttggaacg taaccgtagc cgagtaccgt
    47341 ataataatgc tgctattgcg gacacaggaa atcctgcctg gatatttaac ccaggagtat
    47401 tagagaaaat cgtggccact tcacaggcgc gaggctattt ctctggaaat aactatattc
    47461 ttgaacctgt aaatattatc aatggtaatg gttattacaa tatgtttaaa ttagatggca
    47521 cttatttgtt ctctatcaat gccaagacag gttactttgt aggtaacgct cctggaagag
    47581 ctgatagttt agattattaa tataaagagc aaaaccagtt ttttgtctac accctaaaag
    47641 ttagaaaatc agtctaacga ttagggtgtt ttttgtattc gcatttagat gtgtacgact
    47701 gctatgagga tattagggaa aaattaaaag gattttgctc tctatgatac agaacaaaat
    47761 cctttgttta aattaataag acaacttttg gaattccaaa agttgacttt tattttttat
    47821 tagatcagac aatgtggcgc tcaaaagcat tgtcagaaat cccttgggaa agaattaatt
    47881 tcgcccattc ttgagcagag tggagactgt ggtctttata atttccacaa gattcaatgg
    47941 ttgttccagg aacgtcttcc caagtgaccc caccagcaat agcctctaga ctagatttga
    48001 tgactttagc aatttcagta gcatcctgtt ttccccacat aatcatgtga aaaccagttc
    48061 tacaaccaaa aggagaacaa tcaatcaaac cattaatttg ttggcggatt aacttcgcca
    48121 gaaggtgctc gatagtatgt aaaccagcag tatcaatagc attttcatta ggctgaataa
    48181 ggcggatatc aaagttagta atgatgtctc cgaccggtcc aacttcttct gaaattaaac
    48241 gaacgtaggg tgctttaacg atggtgtgat ctaattcaaa actctctacg acaacttctt
    48301 ttgtcataag ttttctcctt ttgtattaca ttctagagac taaaggagat gttgaaaatt
    48361 tctcctaatc cctttaaata acattctaat catatcataa atgtctataa ttttaaattg
    48421 tgaaaacgcc ttttttatga taaacttaaa agtggacttt ctatatgaaa tctagaaaaa
    48481 aattgaggta taaacatgtt taatattata ttagcaatgg tttgtgcact cattggtttg
    48541 attattggtt atgtggctat ttcaatgaaa atgaaatcat caaaagaagc tgcagaacta
    48601 actcttttaa atgcagaaca agatgccgtt gatttacgtg gtaaagcaga aattgaagca
    48661 gaacacatcc gtaaagcagc ggaacgtgaa agtaaagctc accaaaaaga attactttta
    48721 gaagcaaaag aagaggctag aaaatatcgt gaagagatcg aaaaggaatt taaatctgat
    48781 agacaagagt tgaaacagat ggaggcacgt ttaacagatc gtgcatcatc tcttgatcgt
    48841 aaagatgaaa atctatctaa caaagaaaaa atgttagata gcaaagaaca aagtctaacc
    48901 gataaatcta gacatattaa tgagcgcgaa caggaaattg ctactttaga aacaaaaaaa
    48961 gtagaagaat tgtcacgcat cgcagagctt agtcaagaag aagctaaaga tatcatttta
    49021 gctgataccg aaaaagattt agctcatgat attgcgacac gtattaaaga agcagaacgg
    49081 gaagtaaaag atcgatcaaa caagatagca aaagacttgc tagcccaagc tatgcaacgt
    49141 ttagctggtg aatatgttac tgaacaaaca attacaactg ttcatctccc tgatgataat
    49201 atgaagggac gtatcattgg tcgtgaagga cgtaatattc gtactctgga aagtttgaca
    49261 ggtattgatg ttatcataga tgatacgcca gaagtcgttg tcttatcagg tttcgacccc
    49321 attcgtcgtg aaattgctcg tatgacttta gaatctttga ttcaagatgg acgtatccat
    49381 ccagctcgta ttgaagagtt ggttgaaaaa aatcgtttgg aaatggatca acgtatccgt
    49441 gagtatggtg aggcagcagc ttatgagatt ggtgcaccaa atctgcatcc tgatttgata
    49501 aaaattatgg gacgtttgca gttccgtaca tcatatggtc aaaatgtact tcgtcattca
    49561 gttgaagttg gtaaacttgc aggtatttta gctggagaac taggagaaaa tgttgattta
    49621 gctcgtcggg ctggcttcct ccatgatatg gggaaagcta ttgatcgcga agttgaaggc
    49681 agtcacgttg aaattggtat ggaatttgct cgtaagtaca aagagcatcc gattgttgtt
    49741 aatactattg caagccacca tggtgatgta gaacctgaca gcgttattgc agtaattgtt
    49801 gctgctgcag acgcccttag ttcagcacga ccaggtgcgc gtaatgagtc aatggaaaat
    49861 tatatcaaac gtctgcgtga tttagaagaa attgctaatg gctttgaggg agtgcaaaat
    49921 gcctttgcac ttcaagccgg acgtgaaatt cgtatcatgg tacaacctgg taaagtttct
    49981 gatgatcaag tagttattat gtcgcataag gtacgtgaaa aaatcgaaca aaacttggat
    50041 tatccaggta atattaaggt aacagttatt cgtgaaatgc gagctgttga ttttgcaaaa
    50101 tagtcaaaag gagagcttgt tagctctcct tttgttatag agttgacatt tgtcatattt
    50161 tgaaatgaca aaaatatcta aaaagtttcc ttctgatttt ttatactatg attatagtaa
    50221 aggagaacca aatggcaaaa gatataatta aggttgatca tatatttaaa agcatcgggc
    50281 aaaaaacgat tttagaagat atatcattta gtatagcttc taaccaatgt gtagcattga
    50341 ttggacctaa tggtgcaggg aaaacaactc tgatgtcaac tttattaggt gatattagta
    50401 tttcttcagg agcattgact atttttaatc tgcctgcaca ccacaatagg ttaaaatata
    50461 aggtggccat cttaccacaa gaaaatgtat tgccctcaaa gtttacagtg agagaactca
    50521 ttgatttcca aaggtgttta tttccagaag tgctgccaat gtctctgatt ttagactatc
    50581 tacagtggag tgatgcgcac ttgcaacaat ttacagagac tttatctgga gggcaaaaac
    50641 gacttttagc ttttgtttta actttggttg gtaagcccca attactattt ttagatgaac
    50701 ctacttccgg aatggatact tccacacgtc aacgattttg ggagctggtt gcgacactaa
    50761 aaaaagaagg tgacacaatt gtctattcta gtcattatct cgaagaggta gaacatacag
    50821 ctgataggat tttagtactt cataaaggaa agttattacg cgatacaacc ccctttgcca
    50881 tgaagcaaga aaaaaccgaa aagttattca ccgttccgct tagttatcaa aaattattac
    50941 ctacctattt gattacagag tgtgaagcca agagtgatag tataacgttt gttactgggg
    51001 aggctgaaac tgtatggaaa atactggcag ataatggttg tcctattgaa gctattgaga
    51061 tgaccaatag aactttgtta aatcgtattt ttgagactac taaggaggta aaacatgaga
    51121 atctttaaag cattattgaa aatggaattt cttttgacaa aacgtcaact tgccaatctc
    51181 attatggcta taggtatgcc agtggctttt tttctatttt tttcaggatt tatgggcgaa
    51241 ggactcacta aagccatcga agctatctac gttcgtaatt atatgataac gatggcagga
    51301 ttctcaagtc ttagctttgc tttttttact tttccatttt ctatgaaaga tgatcaactc
    51361 tcaaaccgaa tgcaattatt aagacactca ccagtaccaa tgtggcaata ttatctagca
    51421 aaaattattc gtattttatt ttactattgc ctagcaataa ctgttgtttt cctaacaggg
    51481 catattttaa ggcaagttag tatgccaata gaacaatgga tgcagagctt tttattgcta
    51541 ttaggaggag caacatgttt tattcctttt ggattgctcg tctcctactt taaaaatact
    51601 gaactaatgt caatggtagc caatatttgt tatatgtcat tagctgttct tggtggcatg
    51661 tggatgccaa ttacaatgtt cccaaaatgg ttgcaaggtt tatcaaagtt aacccctact
    51721 taccatttga ctcaagttat ccttagtccg tttgctaata gttttgctgt tttttctctt
    51781 attattctga taggctatgg tattataatg ttagttatcg cttatctact atctcagaag
    51841 aggcattcaa tttgaaaaat aaaaaaaata actatgattt aatgtatttt attccgttag
    51901 tatttttgat ttaccccata ggagggattc tctattatca ctaccctttt tggactttat
    51961 tttttacgct agcttttgta ggagcatatt tatactctat tattatcagg ggcgaatcaa
    52021 agtatcacat gattgcctgg tttacaatgt tgacatatat tttctacatg acagttttta
    52081 taaatagtgg tttcatctgg tatatttact ttctgagtaa tttactcgtc tatcgcttta
    52141 gagataaatt gaagtctttt agattcatta gttttgcttg cactctagcc acaatggtat
    52201 ttttatgctt ctttaaggca tcagactttg gtgatagaat catgtttcta attgttccta
    52261 ttttttgtat cggttacatg tggattgcta ttgaaaatag aaatagcgaa gaacaaagag
    52321 aaaaaattgc agagcaaaat cagtatatca atattttgtc agctgaaaat gaacgtaacc
    52381 gtatagggcg tgatttgcat gatagtttag gacatacatt tgctatgatg acactaaaga
    52441 cagaactagc gttaaagttg ttagaaaaaa ggaactatga taaggtacaa aaagaactat
    52501 cagaattaaa tcatattagt catcaatcga tgagtgaggt ccgtcaaatt gtttctaacc
    52561 tcaagtatcg tacagtagtg gaagaaattg atgagttgta ccgcttgttt caattatcta
    52621 acatcaagtt aacagttgtt aataagttag agacgtcaca actaagtcca gtaacacaat
    52681 ctacaatcac aatgatatta aaggaattat ccaataatat tgttaagcat gctgaagctg
    52741 atagcgtgga gttgagttta gtaaggcaag gtgctactat aaatattgag atgattgata
    52801 atggttgtgg ttttacaaac cttgacggag atgagttgca tagtattcaa gagagattaa
    52861 ctatcgttga agggacacta acgattttgt cccgaagtaa accaactcat attcaggtga
    52921 tattgaaaga ggtataagat gaaattactt gttgctgagg atcagtccat gctacgagac
    52981 gctatgtgcc aattactttt gatggaagaa agtgtttcta ctattgatca agctggaaat
    53041 ggtgaagaag ctattgccat tttatccaat aaagccatag atgttgcaat attggatgtt
    53101 gagatgccga tattatcagg tttagatgtc ttagagtggg ttagaaaata ccagaatgtt
    53161 aaggtcataa ttgtaacaac ctttaagcgg tcaggttatt tccaaagggc tattcgttca
    53221 aatgtagatg cttatgtttt gaaagaccgc tcagtagctg atttgatgaa aacgattcaa
    53281 aaagttttat ctgggggtaa agaatattcg cctgaattaa tggaaaacgt tatatctaat
    53341 cctttgagta agcaagaaat aaaaattctt tccctaattg ctcaagggaa gaccagcaaa
    53401 gaaattgcat gcactttatt tttatcgaat ggtacagttc gtaattacac gtcaaatatt
    53461 tttgataaat taggagttaa taatcgtttg gaggcactta aaatagcacg agaaaaggat
    53521 tggctgtgat agtttttttc cagtcgagat gccataatgt attaagaggg atatatgtat
    53581 gcaaaaattt aaagaacgtt ttgatacgct tagtgatgca attttggcca ttgctatgac
    53641 gattttagtt ttggagataa aaactccgac tacgatgggt gatattggtg actttacacg
    53701 gaacataggt ttatttattg ttagctttgt tgttgttttt aatttttggt acgaaagggc
    53761 acaaaatagc ctagatgctc aaaagactaa tgatgaaatt atagctcttg atatcattga
    53821 acacctaggt atttgtttga ttcctctctt tactaaattt atgatttctt ttgagaatca
    53881 taattttgct gttatggctt atggactctt aacgttatta gttggtctca catcagacat
    53941 tattcggatt cgtttggcaa gctatgattt agtgaccatt cctagtgaat tgaaagagcg
    54001 ggttataaaa gtcatgacaa cttttgccat tcgttcagtt gtggttcgct ttatcattat
    54061 tatcctcgct tattttctac ctgaggtagg gatatttgca tatttagtca taccgctctt
    54121 tatgttttgg cgtcgctcta gagcaggaaa atactatact aaaaagggca ttgaggcacc
    54181 ctcttatatc aagcttttta tccaaattgg agagagagtt cctgaggatt ttactaattc
    54241 aagtagtgga ataagtgggg taaaaagact ctataaaaaa taataggaga ccttgaaaaa
    54301 aaggtttctt tttgttaaac taatgagaag aaactttatt tagggagcaa tttatgaatt
    54361 gctataaaag ttaatagtta aggtaagaac cagattgttc tggtcttttt aacacttaac
    54421 acccgatcta ttaatttgag gagtgtgtaa tgtctgaacg tggtttgcta attgttttct
    54481 caggaccatc tggtgttggt aaggggacag ttaggcaaga aattttttca acccctgacc
    54541 ataaatttga ttattccgtt tcaatgacta ctcgtccaca acgcccaggc gaagttgatg
    54601 gagttgatta tttcttccgt acacgtgaag agtttgaagc gttgattaaa gaaggtcaaa
    54661 tgcttgagta tgcggagtat gttggtaact actatggaac tcctttaagt tatgtcaacg
    54721 agactttaga caaagggatt gatgtttttc ttgaaataga agttcaggga gcgcttcaag
    54781 ttaaatcgaa ggttccggac ggtgttttca ttttcttaac accaccagat ttagaggaat
    54841 tagaagagcg tttagtagga cgtgggacag atagtccaga agtgattgcc caacgtattg
    54901 aacgtgccaa agaagaaatt gctttgatga gggagtatga ctatgctgtt gtcaatgatc
    54961 aagtgtcatt agcagcagaa cgtgttaaac gtgtcattga agcagaacat tatcgtgtgg
    55021 atcgtgttat tgggcgttat accaacatgg tcaaagaaac tgataaaaaa ttaagttaaa
    55081 aatagaaaag agaattgcct tatgatgtta aaaccttcta tcgacacttt gttggataaa
    55141 gttccttcaa aatattcttt agttatttta caagctaagc gtgctcatga attggaagct
    55201 ggtgagaaag ccactcaaga ttttaaatct gtgaaatcaa cacttcgcgc tttagaagaa
    55261 atcgagtcag gtaatgttgt aattcaccca gatccatcag caaaacgtgc ctcagttcgc
    55321 gctcgtatcg aagctgaacg ccttgctaaa gaagaagaag aacgtaaaat caaagagcaa
    55381 attgctaaag aaaaagaaga cggcgaaaaa atttaagcaa gtcaaaagtg agaggttgaa
    55441 acattctgta tcagcctctt ttcatatttt agagaattag ggagggttta tggcaagaaa
    55501 attagcacag gttattgttg atattcctct gatgcagaca gataaaccat tttcgtatgc
    55561 cattcctaaa gatttggaag atttggtaca agtaggagca cgcgttcatg ttccttttgg
    55621 aagagggaat cgtcttttgc aaggctttgt tgtcggtttc agagatgatg atgaattaga
    55681 gactaaggat attgcggggg tcctagattt tgaacctgtt ttaaatcagg aacaactaga
    55741 cttagctgat cagatgcgcc atactgtctt ttcttataaa atatctattt tgaaatcaat
    55801 gttaccaagt cttttgaatt cgcaatatga taaactcttg ttagcaacag acacattacc
    55861 atcagaagat agagaagatc tatttggtca taaaacggaa attgtctttt caagtttgtc
    55921 ttctcaagat gccaaaaaag caggcagatt aattcaaaaa ggtttcatag aagttcaata
    55981 tttagccaaa gataagaaaa caataaaaac agaaaagatt tacaaaatca acagaactct
    56041 gttagaagag agtcaaatag cagcaagagc gaaaaagaga ctggagttga aagaattcct
    56101 gttagaaaat cctcaaccag ggcgcctcac agctttaaac aaacaatttt catctcctgt
    56161 agttaatttt ttcagagaag aaggaatcat tgaggtcatt gaaaaagaag ctagtagatc
    56221 tgataattat tttaaaggaa tcctaaaaac ggattttttg gatttaaatc aggaacaagc
    56281 taaagtagtt aaaatagttg ttgaccgaat tggtaaggat cagaataaac ccttcttgtt
    56341 ggaggggatt acagggtctg gtaagacaga ggtatatctt catgtcattg ataacgtttt
    56401 aaagttggga aaaacagcga ttgttctcgt tccagaaatt tctttaactc cccagatgac
    56461 gaatcgtttt atttcccgtt ttggaaagcg agttgctatt atgcactcgg gcttgtcaga
    56521 tggtgaaaaa tttgatgagt ggcgaaagat aaaatcaggt caagctaaag ttgtcgttgg
    56581 agctcgttca gcaatttttg ctcctttgga aaatatcgga gccatcatta tagatgaaga
    56641 acatgaatca acttataagc aagaatctaa cccaaggtat catgcgcgcg atgtggcctt
    56701 gctacgtgca gaatatcata aagctgtgtt actaatggga tcagcaactc caagtatcga
    56761 aagccgagct agagctagtc gggatgttta taaattttta gaattaaaac atcgtgctaa
    56821 tcctaaagca cgtattcctc aggttgaaat tattgatttt agaaatttta ttggtcaaca
    56881 agaagttagt aatttcacac catatttact agataaaatc cgagatagat tagataagaa
    56941 agagcaagtg gttcttatgc tcaatcgtag aggttattct agttttatta tgtgtcgaaa
    57001 ctgcggctac gttgatcaat gtcctaattg tgacatttct ctgactttac atatggctac
    57061 aaagaccatg aattgtcatt attgtgggtt tgaaaagcct attccaagaa catgtccaaa
    57121 ttgcaatagt aaaagtatct cttactatgg tactggaacc caaaaagctt atgaagaact
    57181 tttgaaagtt atcccagacg caaagatttt acggatggat gttgatacga caaggcaaaa
    57241 aggcggtcat gagtctattt tgaagcgttt cggaaatcat gaagctgata ttttattggg
    57301 aacacagatg attgctaagg gattggattt tccaaatgtt actttagttg gagtactaaa
    57361 tgcagatact tctctgaatt tacctgattt tcgatcatca gagcgaactt ttcagctatt
    57421 aactcaagtg gctggacgtg ctgggcgtgc tgaaaaagaa ggagaggttg tcattcaaac
    57481 ttataatcct aatcattatg ctattcaatt agcccaaaag caagattttg aagcttttta
    57541 tcaatatgaa atgaatatcc gtcgacaact tggttatcct ccttattatt ttacagttgg
    57601 tttaacatta tcgcataaag atgaagagtg gttaatacga aaatcttatg aagtattaag
    57661 tttattgaag caaggcttta gtgataaagt aaagttgtta ggtccaacgc caaaaccaat
    57721 tgcaagaaca cataaccttt atcactacca aattattatc aagtatcgat ttgaggacaa
    57781 ccttgaactt gttttgaatc gcttattaga catcactcaa gacaaggaga atagagatct
    57841 tcgcttagca atagaccacg aaccgcaaaa tatgatgtag aatagtgcta tgatataatt
    57901 gagaaaagaa agacgaggaa aaagcaatga caaaattatt attcatgggt acccctgatt
    57961 tttcagcgac ggttttaaaa ggtattttag cagatggtaa gtatgatgtt ttagctgttg
    58021 tgacgcaacc agatcgtgct gtaggtcgta aaaaagaaat taaaatgacc cctgtaaaag
    58081 aagtggcctt ggaaaacaac attcctgtgt atcagccgga aaaactatct ggaagtccag
    58141 aattagagca actaatgacc ctaggtgcag atggtattgt aacagctgct tttggtcaat
    58201 tcttaccaac aaagcttttg gaatcggtag gttttgcaat taatgtacat gcttctctat
    58261 taccaaaata tcgtggtggg gcaccaattc attatgccat tattaatggc gaaaaagaag
    58321 ctggggttac catcatggag atggttgcaa agatggatgc aggggatatg gtatcaaaag
    58381 cttccgttga gataactgat gaagacaatg tagggacaat gtttgataga ctcgccgttg
    58441 ttggtcgtga ccttttatta gatacgcttc caggttatct atcaggtgat attaagccga
    58501 taccacaaaa tgaagaagag gtaagttttt ctcctaatat tagtccagat gaagagagaa
    58561 ttgattggaa taaatcatcg cgcgacattt ttaaccatgt tcgagggatg tatccatggc
    58621 cagttgcaca tacccttttg gagggaaatc gttttaaact ttatgaagta acaatgtcag
    58681 aaggaaaggg ctcaccgggt caagtgatcg ctaagacaaa gaactcattg acagtagcaa
    58741 ctggtgacgg agctattgaa ctcaaatcag ttcaaccagc agggaaacct cgtatggata
    58801 taaaagactt tttaaatggt gttggtcgta atttagaaat aggagataaa tttggcgaat
    58861 gattggaaaa agagtgcgcg tggtttagct ttaatgactt tagaagaagt ttttgataaa
    58921 ggagcttatt ctaatatcgc acttaataag agtttaaaaa aatctagact atcagataaa
    58981 gatcgtgcct tagtaacaga aattgtctat ggaactgtgg ctagaaaaat aacgctagaa
    59041 tggtatctat cacactttat agttgatcgt gataagcttg aattatgggt atatcatctc
    59101 ttgctactga gtctttatca actactctat ctggacaaca tcccagatca cgctattgtt
    59161 aatgatgctg tcactattgc taagaatcga ggcaataaaa agggtgctga aaagttaatt
    59221 aatgccgttt tacgaagagt aagttcggag acgctccctg aaattgcatc aataaaacgc
    59281 caaaacaaac gttactctgt tgcttattcc atgccagtgt ggctcgtaaa aaaattaatt
    59341 gaccaatatg gtgagacaag agctttagca attatggaga gtttatttga gcgaaataaa
    59401 gccagtcttc gtgtgactga cttatcgcaa aaacaaacta ttaaagaaac tttaaatgtt
    59461 agagattcac atattgcaga gacagcttta gttgcagact caggaaattt tgcatcgaca
    59521 tcatttttcc aagatggcct cattactatc caagatgaat ctagtcaatt agttgctcca
    59581 acactcaaag tctcgggtaa tgaccaagta ttggatgcat gtagtgcacc aggaggaaaa
    59641 acaagtcata tcgcctcata tttgactaca ggggcagtaa ctgctttaga tttgtatgat
    59701 cataaattgg aattggttat ggaaaatgca aaacgcctag gtctgtcaga taagataaaa
    59761 actaaaaagt tagatgcaag taaagctcat gagtattttc ctgaggatac ttttgacaaa
    59821 attttagtag acgcaccttg ttcaggtatc gggctaattc gccgtaaacc agatattaag
    59881 tacaataaag ctaatcaaga ctttgaagcc ctccaagaga ttcaattgtc tatcctatct
    59941 agcgtttgtc aaacgctacg taaaggtggt ataataacct atagcacttg taccattttt
    60001 gaggaagaaa attttcaagt tattgaaaaa ttcttagaaa accaccccaa ttttgagcaa
    60061 gtagaactga gtcatacaca agaggatatt gtaaagagag gctgtattag tataagtcct
    60121 gaacaatatc atacagatgg cttttttatt ggacaagtca aacgtatctt gtagtgtcta
    60181 ggaggaaact gacatcgtca gaaaaaaata tatggaaata tctcttttaa cagatattgg
    60241 gcaacgtcgt tccaataatc aagattttat taatcaattt gaaaataaag ccggcgtccc
    60301 actaatcatc ttagcagatg gtatgggagg acatcgtgca ggaaatattg ctagcgagat
    60361 gactgtaact gatctaggaa gtgattgggc ggagacagat ttttctgagt tatcagaaat
    60421 tcgtgattgg atgcttgtct ctattgaaac tgagaatcgc aaaatatatg aattaggtca
    60481 gtcagatgat tacaaaggta tgggaactac cattgaagca gttgccattg taggagataa
    60541 tattattttc gcacatgtag gagattcacg tatcggtatc gttcgtcaag gggagtatca
    60601 tttattgacg agtgatcatt ctcttgttaa tgaattggta aaagccggcc aattaacaga
    60661 agaagaagct gctagtcatc ctcaaaaaaa tattattacg caatcaattg gtcaagccaa
    60721 tccagtcgaa cctgatttag gagttcatct acttgaagaa ggtgactacc ttgtagttaa
    60781 tagtgatgga ctaactaata tgttgagtaa tgccgatatt gctacagtac taacacaaga
    60841 aaaaacctta gatgataaga atcaagattt aatcactcta gcaaaccata gaggaggtct
    60901 tgataatatc actgtggcac tagtttacgt tgaaagtgag gcggtttaat gattcagatt
    60961 ggcaaattat ttgctggtcg ttatcggatc cttaaatcaa tcggacgagg tggaatggcg
    61021 gatgtttatc tagctcgaga tctaatttta gataatgaag aggtcgcaat aaaagtattg
    61081 cgaaccaatt atcaaacaga tcagattgcg gttgctcgct tccagagaga agcacgagca
    61141 atggctgagc tgacacatcc taatattgtc gctattcgtg atataggtga agaagacgga
    61201 cagcagttct tagtaatgga atatgttgat ggattcgatt taaaaaagta tattcaagac
    61261 aatgcacccc tatcaaataa tgaagttgtt cgaatcatga atgaagtcct ttcagccatg
    61321 tcactggcac atcagaaagg tatcgtacac cgtgatttaa aacctcaaaa tattttatta
    61381 actaaaaaag ggactgtaaa agttacagat ttcggtattg cggtagcgtt tgctgaaact
    61441 agtttaacgc aaacgaattc tatgttaggt agtgttcatt atttatcgcc agagcaggcg
    61501 cgtggttcaa aagcgactgt tcagagtgat atttatgcca tgggaatcat gctttttgaa
    61561 atgttaactg gccatattcc ttatgatggg gatagtgcag ttactattgc cctacagcac
    61621 ttccaaaaac cactaccatc aatccttgca gaaaataaaa gcgttccaca agctttggaa
    61681 aacattgtca ttaaagcaac agctaaaaag ctaacagatc gctataagac aacttatgag
    61741 atgggacgtg atttatcaac tgctctaagc tcgacacgtc accgtgaacc aaaacttgtt
    61801 tttaatgata ctgagagtac taagacacta ccaaaagtaa catctacagt atcatcttta
    61861 acgacagaac aactgttgcg taatcaaaaa caggccaaaa caacagaaaa aataactcct
    61921 gattctgcga gtaacgataa aacgaagtct aaaaagaagg cttctcatcg acttcttggt
    61981 acaatcatga aattattctt tgcactctgt gttgtaggaa taattgtatt tgcttataaa
    62041 attttagtaa gtcctacgac tattcgtgtt ccagatgtta gcaacaaaac agtagctcaa
    62101 gcaaaaatga cattggaaaa ttctggattg aaagtaggag ctattcgtaa tattgagagt
    62161 gatagtgttt cagaaggcct tgtcgtaaaa acagaccctg ctgcaggacg ttctcgtaga
    62221 gaaggggcta aagttaatct ttatattgcc actccaaaca agagtttcac tctaggtaat
    62281 tataaggaac ataattacaa agatatcctc aaagatcttc aaggcaaagg tgttaaaaaa
    62341 tctttaatca aggttaagcg taaaattaat aatgattata ctactggtac aatcttagca
    62401 cagtcgcttc cagaaggaac atcttttaat cctgatggta ataagaaatt aacacttacg
    62461 gttgctgtta atgatccaat gattatgcct gatgtaacag gtatgacagt tggagaagta
    62521 atagaaacct tgacagacct cggattagat gcagataatc ttgtcttcta tcaaatgcaa
    62581 aatggcgttt atcaagcagt agtaacacct ccatcttcaa gtaagatagc aagtcaggat
    62641 ccttattacg gaggagaagt tggattaagg agaggagata aagttaagct gtatcttctt
    62701 ggttctaaaa caacaaataa ctcatcatct acgccaatag attcttcggc ttcaagctca
    62761 acaggaacaa ctacctcgga ttctgtaagc tcatcaacgg atgctagcac ttctgatagt
    62821 agtagcactt ctacaagtag ttcaacacta ccaagtgata gtacaactaa tacaggaaca
    62881 gctaacaatc cattaactca ataaaattaa aaagaagatg ttaaagtctt ctttttaatt
    62941 tgctatggta gagaccatgc ctagaaaggt tctgccaata tgccaaattt ttctctcaat
    63001 aatggtgcaa agtatagggc tttttgataa aatacgtcat atagagtaat ggaaggatta
    63061 ttatgaaaaa atttcagttt tttctacttg ttgaagccgt tgtcttagtt atgggattaa
    63121 tgaaaatttt atcagatgac tggacaagtt ttatttttat tctcgctttg attcttttag
    63181 ccttaagatt ttataacaac gatagtcgtc ataattttct gttaacgaca agtttgttat
    63241 tactattttt aatctttatg cttaaccctt acattattgc agccgttgtt tttgcagttt
    63301 tgtatgttct aatcaatcat ttctcgcaag ttaagaaaaa aaatcgttat gctttgattc
    63361 aatttaagaa ccatcaatta gatgttaaga caacacgaaa ccaatggtta ggtactgatc
    63421 agcacgaaag tgacttttat gcttttgaag atattaatat tataagaatc tctgggactg
    63481 atacaattga tttaacaaat gttattgtat ctgggcagga taatgttatt ataattcaaa
    63541 aagtttttgg tgataccaaa gtattggttc ctttagacgt tgccgtaaag gctgatatta
    63601 gttctgttta cggtagtgta cagtactttg attttgagga gtatgatcta agaaatgaat
    63661 ctataaaatt gtcacaagag gaagaatatt accttttgaa acgtgtcaaa ttagttgtaa
    63721 acactatcgc tgggaaagtt gaggttagtc gtaaatgaaa aagcaccact actttttagc
    63781 tttcttctat ggaagtgtta ttatatttgc catttgtttt gtcattattg atagtttggg
    63841 tgttaatttg gtgcacctct atcaaacgtc tcgtttgtgg ctgattgaac aactgatttt
    63901 ctctattttc tttctatctt tagcggtaac tatcctttta ttgctaactt ggtttttatt
    63961 agacgataat agtaaacgtc aaattaacca taatttacgt cgcattttaa ataaccaatc
    64021 aataaatgtt accgatgatg gtactgaaat atcgaccaat attcaacggc tctctaaaaa
    64081 aatgaattta atgacagcaa gtctacaatc taaggaaaat agtcgtattt taaaaagcca
    64141 agaaattgtg aaacaagaac gtaagcgtat tgcacgtgat ttacacgaca cagttagtca
    64201 ggatttgttc gcagcttcca tggttttgtc agggattgca cagaatgttt ctcagctaga
    64261 cgttgatcag gttggctcgc aattattagc agtagaggaa atgcttcagc atgcacaaaa
    64321 tgatttgaga attctgctac ttcatttgag gccagttgaa ttagaaaata agaccctatc
    64381 tgaaggattc aaaatgatat tgaaagaatt gaccgataag agcgacattg aagttgttta
    64441 tcacgaatca attctaacct tacccaaaaa gattgaagac aatattttta gaattggtca
    64501 agaatttatc agtaatactt taaagcattc acaagccagt cgtctcgaag tgtacctcaa
    64561 tcaaacagag aatgaattgc agttaaaaat gattgataat ggtattggat ttgatatgga
    64621 ttctgtctat gatttaagtt atggattaaa aaatattgaa gatcgagtag aagatttagc
    64681 aggaaattta cagcttctca gtcagcctgg taaaggagtt gctatggata taagattacc
    64741 tttagtaaat cagagtgagg ataaaaatgg ataaaataaa aattgtactt gttgatgacc
    64801 atgagatggt tcgtctcggt ttaaaaagtt ttttaaactt acaagctgat gttgaagtaa
    64861 taggtgaagc ctcaaatggt ttagaaggca taaaaaaagc actggagtta cgtccagacg
    64921 ttgttgttat ggatttagta atgcctgaaa tggacggtgt agaagcaacc ttagcattgt
    64981 tgaaggattg gccagaagct gctattttag tcttaacatc ttacttggac aatgaaaaaa
    65041 tttaccctgt tattgaagca ggtgccaaag gctatatgtt aaagacctca agtgcagcag
    65101 agattcttaa tgccattcgt aaagttgcaa ggggagaaca agctattgaa aatgaagtag
    65161 ataagaaaat taaggctcat gataaatgtc cagctttaca tgagggctta acagcaaggg
    65221 aacgtgatat ccttaattta ttagccaaag gttatgacaa tcaaaggatt gctgatgaat
    65281 tatttatctc gttaaagact gttaaaactc atgtttctaa tattttggga aaactaaatg
    65341 tggcagatcg aactcaagcg gttgtctatg ctttccaaca ccatttggta ccacaagatg
    65401 atgaataagt gatataatag agacactatg ttaatgaaag tggttagtca tggacgcaaa
    65461 aactaaatat aaagcaaaaa aaataaaggc ggtgtttttt gatattgatg acacgcttcg
    65521 tgttaaagat acaggttata tgccaccatc gatcttaaaa gtttttaaag ctttgaaaga
    65581 taaaggaatt gttgtaggta ttgcttcagg aagggcacga tatggcgttc caaaagaagt
    65641 tcaagattta aatgccgatt attgtgtaaa attaaatgga gcttatgtta aggacaagga
    65701 taagaatatc atcttccatc gtcctattcc tgctgaatat gtagagcaat ataagaaatg
    65761 ggcagacact gttggtatta agtacggttt agctggtcgt catgaggcag ttttatcaga
    65821 tcgtgatgat ttggtaaatg atgcgattga tattgtgtat tctgatctcg aagttaatcc
    65881 agatttcaat aaagaacatg atatttatca gatgtggacg tttgaagata aaggagatag
    65941 ccttcatcta cctgaaccgt tagatgaaca tcttcgcttg atacgttggc atgatcattc
    66001 ttcagatgtt gtgttaaaag gaacatcgaa ggctttaggt gtttctaaag ttgttgaaca
    66061 tttgggctta aagccagaga atatcttggt tttcggagat gagctcaatg atttagaact
    66121 ctttgattat gctggactag ctgtggcgat gggagtgtca cacccagaag ctcaaaaaaa
    66181 agcagatttt atcacaaaaa aagtagaaga agatggcatt ttgtatgccc tggaggaact
    66241 aggtttgata gaaaaagaat taacatttcc acaagtagat attgaaaata cagaaggtcc
    66301 tgtggctgtc attaaaacaa atcacgggga tatgactgta aaactcttcc cggatcatgc
    66361 tcctaaaacg gtagcaaact ttattggttt agctaaacaa ggttattatg atggtattat
    66421 cttccatcgt attattccag attttatgat tcagggtggt gatccaactg gtacaggtat
    66481 gggtggtgaa tctatctatg gggaaagttt tgaagacgaa ttttccgaag aattatataa
    66541 tgttcgcgga gctctttcaa tggctaatgc tggaccaaat acgaatggca gccaattttt
    66601 catcgtccaa aatacaaaaa ttccttatgc gaaaaaggaa ttagagcgag gtggttggcc
    66661 aacaccaatt gctgagttgt atgctggaca aggtggtaca ccgcatctag atcgtcgcca
    66721 cagtgtattt ggacaattag taaaccaaag ttcttttgag gtacttgatg agattgcggc
    66781 agtagagact ggttctcaag acaaaccatt ggaagatgtt gttattttaa cgattgaggt
    66841 taaagaataa aatgaagatt ggcgataagt taaagggaac agtcactggc attaggcctt
    66901 atggagcctt tgtttctctt gaggatggta ggactggact tatccatatt tctgaaatta
    66961 aaacaggtta cattgataat atttatgatg ttcttagtgt aggtgatgaa gtctatgttc
    67021 aggttataga tgtagatgaa tttacacaaa aagcaagttt atcgcttcgg acccttgaag
    67081 aagaaagaca tcatattcag catcgtcatc gattttctaa caatcgtctt aagataggtt
    67141 tcaaaccact tgaggaaaat ttacctagtt gggttgagga aggtctagcc tacttgaata
    67201 ataaataaac aaaaacctag atttgattaa tgacaagtct aggttttaaa tatttaaaag
    67261 tagcagtgaa tattatactt gctaaaaata gcttgataat cgagtagatc ctctgggtgt
    67321 agtgcagcga tctcttccat ttcatattga cgattaagta atcgatattt gttttgtcca
    67381 aattggtgga aaggtaagag ttgcacttga cggatatcta ggcgatcaaa taaacaagca
    67441 aattcttcag catcttctaa tgaatcgtta aaattaggta tgacaggaat acgtagcaca
    67501 atcgttttac cgttggcaaa agcataatga atgtttttga tgattgaagc attcttaacc
    67561 atcgtctttt cttggtgttt gaggctattg taatgtttta aatcggtgta aataaaatca
    67621 acgtattgga taaggtctat gaattgttca tgtctagtat aagcagttgt ttcaatagcc
    67681 gtgtggattc ccagtgattt agcacgtttt aaaattgcct tggcaaattc aaattgtgca
    67741 aatatttcac cgccagatag cgttatgccc ccaccagatt cttcgtaaaa atcaatatct
    67801 tttaaaacct cttcaataat atcatcaacc gatttttctt cgcccacgat gacgctttca
    67861 ttagtaatgg catctctcat tgtttctgga accatttttt gcgactcggg attagcacac
    67921 caaggacatc taagaggaca accctttaag aaaacggttg ttcgaatacc tggaccatcg
    67981 tgaatactaa aatgttgaat gttgaacaca ataccttttt catttgtcat attattttcc
    68041 tcaatttcat tttaagtata ccttttatac gtaacaaagt caattacgaa tgaaagttta
    68101 ttgaacttgt ttaataaata aaaaatgata aaatagaaag gaggtgtttc atgaatagat
    68161 tagaaaatat tattagttta gtatcccagt accaaaaaat agatgttaat acactctcgg
    68221 agttgttgca agtttctaaa gtaaccatac gtaaagattt agataaacta gaaggcaaag
    68281 ggcttttgca tagagagcac ggttatgcag ttttaaacag tggggatgat cttaatgtta
    68341 ggttatcttt taaccataaa actaaaaaag aaattgctgc cttagctgca aatatggtgt
    68401 ctgacaatga cactatctta atagaatcag gctctacttg tgctctttta gcggaaaata
    68461 tctgtcagac aaagcgaaat gtcactattt tgaccaactc ttgctttatt gctaactatt
    68521 tacgagagta tgattcatgc caaattgtct tacttggtgg tgagtatcaa tcaagttcac
    68581 aggttacggt aggtcctctc ttgaaaaaga tgatttcact ttttcatgtc tcgttagctt
    68641 ttgttgggac tgatggtttt gatccaaaac taggatttat gggtagagat ttaatgcgtt
    68701 ctgaagttgc acaagagatg gcgaatgctg ccgatgaagt cattatttta acagattcta
    68761 gtaagtttaa tcagactgca ttggtggaac agcttccgtt gtcaactgta tcacaagtta
    68821 ttacagataa acatccaaat tcggatattg ctaacttgtt ccaagaagca gagattacca
    68881 ttcaatcttt attacaagta ccataaaaga ggctattttt cttacatttt gcacatttgt
    68941 ggcgataata gataaagtgt catgtcaaat tgcacagatg taaccttttt gttaaactga
    69001 agcaaatgct gaaaggttat gagtatgaaa ttagagcgtc aaaaattgtt agctaaagta
    69061 gcttaccttt actatatgga agggaaaagc caatctgaga ttgccaatga attaggaatt
    69121 tatcgtacaa caataagtcg tatgttagcc aaagcgagag aagaaggtct tgttagaatc
    69181 gaaatttcag attttaatcc agaaattttt cagttagaat cctattttaa aagtaagtat
    69241 caccttaaag atattgagat tgtttcttcg cgaaaagata gcgatacttc agagatagag
    69301 aaggatttag cacatgtagc tgcagctatg attcgaaaaa aaattaaaga gaatgacaag
    69361 gtggggattg cttgggggag gacacttagt aaagtagttg aagcaatgcg tcctcatccg
    69421 gtttctcaag tttcttttgt tcctttggcc ggaggtccaa gtcatattaa tgcacgctat
    69481 cacgttaata ctctggttta cgaaatgtca aggcgtttcc aaggaagttg tactttcatt
    69541 aatgcaactc tagttcaaga aaacgctaat ttagcgaaag gaatattaac ttcaaaatat
    69601 tttgaggggc taatggataa ttgggaaaaa cttgatgtgg caattgtggg cgttggtggt
    69661 aaacctaaaa gcaatgaaca acagtggctt gatcttttaa atcaagatga ttttcaatgc
    69721 ttggatgaag aagcagctgt aggtgaaatt acttgtcgtt tttttaatca ttcaggtgat
    69781 tctgtgaatc aacatttagc taaaaggaca ataggaatta ccttagaaca actacagaaa
    69841 gttcctaata gaatagcggt tgctcatggc aattataagg ctgcggcact cttagccgtt
    69901 ttaaaaaagg ggtatattaa tcatttagtt actgattttt ccacagcctt aaatatttta
    69961 agattagata aagacacctt tgtggatact atgtatcaaa agagctagga tttattccta
    70021 gctcttttaa taatgtttct attaccaagt gaattgttat agaataagca caaaagtgcg
    70081 ctattatcat aaagggcaca aggtctattg acgacgatta agggacatgt tacatttgtt
    70141 ttgcgaaaga aaattagaac cttttgtaag aaagtttaaa cttataaaaa atcattaaag
    70201 cggaggtgct tatggaaatc atagtcgcag accaaattat tatggggctc attttgaatg
    70261 ctggggatgc taaacaacat atctatcaag ccttaaaact tgcaaaagaa ggaaattttg
    70321 cagaaagtaa aattgaaata gaactagcag atagtgcact cctagaagct cataatcttc
    70381 aaacacaatt ccttgcacaa gaagcaggag ggacaaggac agatatctca gcactcttta
    70441 ttcattctca ggatcatttg atgacaagta ttactgagat taacttgatt aaagaaatta
    70501 ttgatttacg ccaggaatta catattcaaa aataagtaaa ggggatcaaa tatgataaaa
    70561 attggtttat tctgtgcagc aggtttttca acaggtatgt tagtaaacaa tatgaaaatc
    70621 gcagcggata aagaagggat agaggcacat attgaagctt attcacaagg aaaaattgca
    70681 gattatgcta aagatttaga tgttgcatta cttggtccac aagttagcta tactctagat
    70741 aaatcaaaat caatctgtga tgaatacggt gtaccgatag cagttattcc aatggcagat
    70801 tatggtatgt tggatggtgt aaaagttttg aaacttgctt tgagtttatt ggaaacaaga
    70861 aaagaggctt gatatgtcaa agtttgatag tcagaaaata attactccga ttatgaagtt
    70921 tgtcaatatg cgagggatta ttgcactcaa agatggcatg ctagcaattt taccactaac
    70981 agttgttggg agtctctttt taatattagg gcagcttcca tttaaaggtc ttaatcaagc
    71041 catagctaat gtttttggac cagaatggac agaaccattt atgcaagttt attcaggaac
    71101 ttttgcgatt atgggcttga tttcctgttt tgcaattgct tatgcttatg ctaaaaatag
    71161 tggtgtagaa ccgttacctg ctggcgtttt atcactttcg tctttcttta ttttaatgaa
    71221 atcatcttat attcctgtaa aaggagaagc aatagcggac gctatttcta aggtttggtt
    71281 tggcggtcag gggattattg gagcaattat tattggctta gtagttggtg ctatctatac
    71341 atggtttatc caacatcata ttgttataaa aatgccagag caagtaccac aggcaatagc
    71401 aaaacaattt gaagctatga ttccagcttt tgttatcttt cttttatcga tgattgttta
    71461 tttgattgct aaggtaacaa ctggaggtac ctttattgag atgatttatg atatcattca
    71521 agtaccgttg caaggcttaa caggctcact ttatggagca attgggattg ctttctttat
    71581 ttcattctta tggtggtttg gtgtccatgg ccaatctgtg gtgaatggta ttgtgactgc
    71641 tttgttacta tccaatttag atgccaataa gtctttatta gcagcaaatc gcttaacatt
    71701 agataatggc gctcacattg taacacagca gtttttggat agtttcttga ttttatcggg
    71761 ctcaggaata acttttggac tagttattgc gatgcttttt gcagcaaaat caaaacaata
    71821 caaggcactt gggaaagtgg ccgcttttcc cgcaattttc aatgttaacg agccaatcgt
    71881 ctttggcttt ccaatcgtaa tgaatcctgt gatgtttctg ccttttattt tagtaccagt
    71941 tttggcagct ttaattgttt atggagctat tgcggttggt tttatgcaac cattctcagg
    72001 tgttacttta ccatggagta ctccagcaat tatttctgga ttcatggtag gtggttggca
    72061 aggtgcactc gtccaaatag tcattttagc tatctcaact gctgtttact tcccattctt
    72121 taaaatccaa gataatatta cttacaaaaa tgaatgtgaa atggaaaggg gatagctagc
    72181 agataagagg ttgaggtgtg taatgcacct taaccttttt ctaaaaatgc acatttgtgc
    72241 aactttagat gtagcattct attctttatt ttcttcttgt tattttagga tttaggtgct
    72301 attatattta cgaagaaaaa ctttttagtt tcgaaagaaa caagcggagg ctgttatgac
    72361 caaacaatta ctcactcaaa aatatacaaa tgatgttcaa caaaattctc aaaagcattt
    72421 tggctatttg acagagcgca tgtattctta tcgcgataag gttcttgata agaaaccatt
    72481 catagatgca gaacgtgcta tcttggtaac tgaagcctat caaaagcatc aggaaaaacc
    72541 taatgtttta aagagagctt acatgttgca aaatattttg gaaaaaatga caatctatat
    72601 tgacgatgag acaatgattg tcggtaacca agcttcatct gataaagatg ctcctatttt
    72661 tccagaatac actctagaat ttgttgtaaa tgaactagac ctttttgaaa aacgagatgg
    72721 tgacgtcttt tacattacag aagaaaccaa agaacaaatt agaaatattg cacctttctg
    72781 ggaaaataat aatttacggg ctcgtgcggg cgttatgctt ccagaagaag ttcaagttta
    72841 tatggagaca ggcttctttg ggatggaagg taagatgaat tctggggatg ctcacttagc
    72901 tgttaactat cagaagttac tagaagaagg gcttattggc tttgaaaaaa aagcaagaaa
    72961 agctaaagct gacttagatt taacaaagcc tgagagtatt gacaaatatc atttctatga
    73021 ttctatttta attacaatag aagctgttaa aacctatgca gagcgttttg ctatcctagc
    73081 aaaaaaacaa gctaaaacag caaatactaa gagacgtcag gaattgttag atatcgctag
    73141 tatttgtgaa cgagtgccat attacccagc tgaaacgttt gcagaagctg ttcaatcggt
    73201 ttggtttatt cagtgtattc ttcaaataga atcaaacggt cattctctat cttatggacg
    73261 ctttgatcaa tacatgtatc catatgttaa agctgactta gaagctggac gtgagactga
    73321 ggattcgatc gttgagcgcc taactaacct atggattaaa acaataacta ttaataaggt
    73381 tcgtagccaa gctcatacgt tttcgtctgc tggtagtcct ctttaccaaa atgtaacgat
    73441 tggaggacaa acccgacata aggaagatgc tgttaatcct ttatcatttc tagtactaaa
    73501 atcagtcgct cagacacact taccacagcc taatctaacg gttcgttatc atgctaacct
    73561 agacaagtca ttcatgaacg aagctattga agttatgaaa ttaggttttg gtatgccagc
    73621 ttttaataat gatgaaatta ttattccatc atttattaaa aaaggagttt ccgaagaaga
    73681 tgcctatgat tattcagcga ttggttgtgt tgaaacagca gttcctggga aatggggtta
    73741 tcgttgtacc ggcatgagct atatcaactt ccctaaagtg ttattaatta caatgaataa
    73801 tggcattgac ccggcttctg ggaagagatt tgcgccttct tatgggcatt tcacccagat
    73861 gacctcatat aaagaactta aagaagcttg ggacaaaaca cttcgttact taactcgaat
    73921 gagtgttatt gttgaaaatg ctattgatat tagtctcgaa agagaagtac ctgatattct
    73981 ttgttcagct ttaactgatg attgtattgg acgaggtaag catttaaaag aaggtggtgc
    74041 agtatatgac tacatatctg gtctacaagt tggaatcgct aatctttcag actcacttgc
    74101 tgcgcttaaa aaattagtat ttgaagaaaa acgcctaacc acattagaag tatggcaagc
    74161 attgcagagt gattacgcag gtccgcgtgg tgaagaaatt cgtcaaatgt tgatcaatga
    74221 agcaccaaaa tatggaaatg atgatgacta cgcggatagt ttagtacgtg agtgttatga
    74281 tgtttatgtt gaagaaattg ctaaatatcc caacacacgc tatggacgtg gaccaatcgg
    74341 tggtattcgc tattcaggaa cgtcctctat ttcagcaaat gtaggtcaag gacgaggtac
    74401 tttagcaact ccagatggta gacatgcagg aactccatta gctgaaggtt gttcaccatc
    74461 tcataatatg gacaaaaagg gacctacctc tgttttgaaa tcagtttcga agttaccaac
    74521 tgatgagatt gtcggcggtg tactccttaa ccaaaaagtc aaccctcaaa ccttggcaaa
    74581 agaagaagat aaacaaaaat taattgcctt acttagaaca ttcttcaatc gccttcatgg
    74641 ttaccatatc cagtataatg ttgtctcacg tgagactttg attgatgctc agaagcaccc
    74701 tgaaaaacac cgcgatttga ttgtgcgtgt agcaggttat tctgcattct ttaatgtcct
    74761 atctaaggca actcaagatg atattattgc acgtacagaa catgctcttt aggagattat
    74821 tatggaattt ttacttgaca ctttaaattt agaagctata aaaaaatggc atcatatact
    74881 gccactagca ggtgtaactt caaatcccac cattgctaag aaagaaggag atatacattt
    74941 tttccaacgc attcgtgatg ttcgagaaat tattggatgt gaagcaagtt tgcatgttca
    75001 agtagtggcc aaagattatc aagggatact ggatgatgct gctaaaatac gccaagaaac
    75061 ggatgatgac atttatatca aggttccagt tacgccagat ggattagcag caattaaaac
    75121 gttaaaagca gaaggttata atattacagc cacagcaata tacaccagta tgcaaggtct
    75181 tttggctata tcagctggag cagactacct agcaccttac tttaaccgaa tggagaacct
    75241 tgatattgat gcgacacaag ttattaagga attagcgcaa gcaatagaga ggacaggttc
    75301 ttctagcaaa attttagcag ctagttttaa aaacgcttca caagtaacga aggccttatc
    75361 acagggtgct caatcaatca ccgcgggacc agatattttt gaatcagtct ttgcgatgcc
    75421 atctattgca aaagctgtta acgactttgc agatgattgg aaagccagtc aacactcaga
    75481 acatatttaa cagagaacgc caacttttga ggctgtatct ttaacataaa attttttaaa
    75541 gaagaaagag gttgagaatg aaagttttcg caagtccatc gcgctatata caaggaaagg
    75601 atgctttatt ccaaagtatt gaacatatca aatctttagg tcagactcca cttatccttt
    75661 gtgatgatgt tgtttataat atcgttggag aaaggttttt atcttatcta caagacgatt
    75721 tgttaccaca tcgtgtctcc tttaacggag aagcatctga taatgaaatc aatcgtgtcg
    75781 ttgctgttgc aaaagaaaag aatagtgacc ttattattgg acttgggggt ggaaaaacta
    75841 ttgactctgc aaaggcaatt gcagataaag ttaatttacc agtgatcatt gcaccaacag
    75901 tagcttcaac agatgcccca acatcagcgt tatcagtaat ctatacagat gaaggagctt
    75961 ttgaaaaata tattttttat tcaaagaatc cagacttagt tttggtcgat actcaagtca
    76021 ttgctcaagc gccaaaacgt ttactagctt ctgggattgc agatggctta gctacttggg
    76081 ttgaagcgag agcagtctta caaaaaaatg ggatagcgat ggctggcggt agacagactt
    76141 tagcgggcgt ggcaattgct caagcatgtg agcgtacttt atttaatgat agtttacaag
    76201 ctttagcggc ttgtgatgtc aaagtggtta caaaagcttt agaaaatgtt attgaagcta
    76261 ataccttact tagtggttta ggttttgaaa gcgcaggatt agctgcagct catgctattc
    76321 ataatggttt tactgccttg agtggagata ttcatcactt aacccatggt gaaaaagttg
    76381 cctatggaac gttgacacaa cttttcctcg aaaatagacc aaaggaagaa attgatcgct
    76441 acattaatct ttaccaagcg attggaatgc caactacact tgcagaactc catttgggtg
    76501 atgcaacata tgaagaactt ttaaaagttg gtcagcaagc aacaattgaa ggtgaaacaa
    76561 tacatgaaat gccattcaaa attagtgcag aggatgtcgc tgcagccctg ttagcagttg
    76621 accgctatgt ttcaaatcat caataaaaac aatagaacca agtgaaattg cttggttcta
    76681 tttgtcgttt taatatttta actaaatagg gcttactagt atttaaagag atataaacta
    76741 aaaaagatca gacaacatat ctaatctttt ttagttatcg tttactgaag attaaagtta
    76801 ttcaaaatca tacaggcttg ttgataagta acgttcgcca ttatcaggga gaagagctaa
    76861 aacctttttc ccttttccta gttgtttggc cacttcaata gcagcataaa tagcagctga
    76921 agcagaaatt cctgcgagga aaccttctag tccgccgatg atacgtcctg tttcaatagc
    76981 atcgttagat gtaacgcgaa tgatatgatc ataagaatct gtagccaatg tttctggaat
    77041 gaaacctgca gaaatccctt gtattttatg aggcccaggt tgctcaccag aaaggattgc
    77101 agattcatca gcttctacag cataaatagc gacatcagga ttgacttttt ttaatgcacg
    77161 agagacacca gtaattgtcc caccagttcc cacacctgcg ataaaagcat ctaaaccttt
    77221 ttcaccaaaa gtctcgataa tttccctacc agtcgttttt tcgtggattt ctggattggc
    77281 ttgattatta aattgcaggg gtagccaagc attttgctct gcagaaattt cttttgcctt
    77341 agcaatagct cccttcatcc cttcactacc aggtgttagt actaattgag caccataggc
    77401 ttgaatgatt ttacgacgtt caattgacat ggtttctggc ataacaataa taacattata
    77461 ccctttagct tttcctaccc aagccaaacc gatacctgta ttaccagatg taggttctac
    77521 gattgtatct ccggctttaa gaatcccttt ttgctctgca tcttcaatca tagctagagc
    77581 gatacgatct ttgacagatg aacctgggtt aaaggattct aacttaacgt acacctctgc
    77641 agctccttct ggaacaatat gatgtaattg aataatgggt gtatttccga ttaagtctgt
    77701 aatagaatta taaatttttg acataagcta tacctcttca tgacattggt taagaatatt
    77761 ataaaactca cattgattaa agtaaaatat caattcttta tcaagtgata gctattattt
    77821 atcggtttca gccttaaagg gaatttcaac aatttgactc ccttcttcag ttaacaaagc
    77881 ttttccttgg tagaactctg tcaaattagt tattgtgtgt tcaaattcct tagggtcaac
    77941 gtatatttta caggttacag cttctaaaaa ttctgtttca aactcctgaa ggtggtgctc
    78001 ttttaggaag ttgtcaaaag tttgatattg aggataagtt aattgaatac gaataccaat
    78061 ttgttcttta acttctacta ccccgatctc tttgatggca ttagctacgc taccagagta
    78121 ggcacgaata agcccccctg tgcctaattt gatacctcca aaataccgtg taacgacggc
    78181 aacaacattt gtcaggcctt gcttttcaag tacggttagc atagggatac ctgctgtacc
    78241 actaggttca ccatcatcac tagatcgttt tatatcccct ttttcgccaa taaccatagc
    78301 tgaacacgaa tgattggctt tgtagtgcgc tttcttgatt tgcgtaatat aattacgccc
    78361 ctcctcttcg gactctactc gctttagatg acaaataaat cgcgattttt taatttcttc
    78421 ttcgacaatt ccgtctgatt ttatcgtttt atagttcata gagaaatttt acaaaaattc
    78481 ttcaaattta gcaaaaaaat ccgaatgaaa taatatggaa aattatttag gtagactttg
    78541 gacaaaagca caattatctg agcaacttag aaaaattgca attaacttgc catcttttat
    78601 aaagaaagga agcgactata tttgtactcg atgctctagt tctgttgcca aaaattgcca
    78661 attaccaact ggaaattatt attgtagaga atgtatagtt tttggaagag ttactagtaa
    78721 tgaaaattta tattatttcc cacaaaaaac attttctaaa acgaattctt taaaatggaa
    78781 agggggactg acaccttatc aaaatgaggt ttcagaagag ttgttgaaag gtatttcaag
    78841 taaggaaaat cttcttgttc atgctgtgac aggggctggt aaaacagaaa tgatatacca
    78901 ctctatcgcc aaagtaattg atactggagg gagcgtttgt attgccagtc ctagaattga
    78961 tgtttgttta gaattgcata agcgactttc aaacgatttt agatgtgcca taacattaat
    79021 gcatggagaa tctccttctt atcagagatc ccctttaact attgccacaa cacatcaatt
    79081 gttaaaattt tatcatgctt ttgatttgct aattgttgat gaagttgatg cttttccata
    79141 tgtcgacaac ccgatactat accatggtgt aaaacaggcc ttaaagaaaa acggaacaag
    79201 tatttttctg actgcaactt caacgactga gttagaaaga aaagttgcaa gaaaagaact
    79261 aaaaaaatta catctagcta ggagatttca tgccaatccg ttagtgatac ccgagatggt
    79321 ttgggtaagt ggtatccaga aaagcttgca gacacaaaaa ctacctccca aattatatca
    79381 gctgataaat aaacagcgac aaacgagata tcctttgtta ttatttttcc cccatattgc
    79441 agaaggtcag gtattcaccg aaatgttaag acaagccttt cctatggaaa aaattggttt
    79501 tgtttcaagt aagagcacat ctaggttaaa gttagtgcaa gattttcgag ataacaaatt
    79561 gtctatccta gtatcaacta ccattctaga gagaggagta actttcccta gcgtcgacgt
    79621 ttttgttata caagcaaatc atcatttgtt tacgaaaagt tccttggtac aaatttcagg
    79681 aagagttgga agagcattag aaagacccga agggctcctt tacttccttc atgatgggaa
    79741 aagtaaatcg atgcaccaag ccataaagga gattaaaaac atgaatcata taggaggatt
    79801 ttaatgactt gtttgttatg tcatgaaata gatttatcgc aattaacttt tgtggaactt
    79861 atacttctaa aaccaaagca aaacgtaatt tgtcaaacgt gcaagggtag ttttgaagca
    79921 cttagtaggg agatgggttg ccaaacatgt tgcaagcaaa tcctccgaaa acaatgtcaa
    79981 gattgtattt attggggtaa aaaaggtata gaggtaaatc atttttccct ttatagatac
    80041 aatgaagcaa tgaaaaaaat atttttctct ttttaaattt caaggggatt atttgttgaa
    80101 agatgttttt acaaaagaaa taaaggctgc tctaaaaagg tataagggct acactatagt
    80161 gccagttccc ttgagtcatg aaggatacca aaataggcaa tttaatcaag tgattgcttt
    80221 tctacaatcg gcaaatatac cttataaaaa tattctttct aaaaaagatg gaggtaagca
    80281 atcggctaat aataaagaag aaagactcaa acaagttcag caatttacat taaaaaatga
    80341 ggctgagtta agggataacc ttttaattgt tgatgatatt tatacaacag gcgcaacaat
    80401 agcacaaatc aggaaactat tagaagaaaa aggtataaaa aatataaaaa gtttttcatt
    80461 agcacgctaa aatcttgcaa attttcatga aagcggttat aataagaata tagaaagcgt
    80521 taacaacgca agaaagaggt actcatatga ttaaatacag tattcgtggt gaaaacatcg
    80581 aagtaacaga agctattcgt gagtatgttg agaccaaact ctctaaagtt gaaaaatact
    80641 ttaatgaaga acaagaatta gatactagag tcaacctcaa agtatacaga gagaagaccg
    80701 caaaagttga agtaacaatc ttaatagatt ctattacttt acgagcagaa gatgtttcac
    80761 aagatatgta tgggtcaatt gacttagttg ttgataaaat tgagcgtcaa attcgtaaaa
    80821 ataaaactaa aattgcgaaa aaatatcgtg aaaaaattcc agcaagtcaa gttttcacaa
    80881 cagaatttga ggctgagcca gatgaagaag cagtaagcca aagaattgta cgtactaaga
    80941 atgttaatct aaaaccaatg gatgttgagg aagcattgtt gcaaatggaa cttttaggtc
    81001 atgatttctt tatctataca gatgcagaag ataatacgac aaatgtcctt tacaagcgtg
    81061 aagatggcga actaggttta attgaagcta aaggaaatta actttgtaac ataatttaaa
    81121 aaatctcaga gttttttctg agattttttt aaaacctatt gacaacaaat aaaaaattat
    81181 agtatactaa atgagttctt aagcgaacct aatattgtat tatcatttta taaacaactt
    81241 aaaaaaagt
//
LOCUS       DATRME010000003        59925 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRME010000003 DATRME010000000
VERSION     DATRME010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59925
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(122..412)
                     /locus_tag="VBC23_000168"
     CDS             complement(122..412)
                     /locus_tag="VBC23_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6842149.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            complement(867..1259)
                     /gene="rpsI"
                     /locus_tag="VBC23_000169"
     CDS             complement(867..1259)
                     /gene="rpsI"
                     /locus_tag="VBC23_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6842150.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1280..1726)
                     /gene="rplM"
                     /locus_tag="VBC23_000170"
     CDS             complement(1280..1726)
                     /gene="rplM"
                     /locus_tag="VBC23_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6842151.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(1948..2070)
                     /locus_tag="VBC23_000171"
     CDS             complement(1948..2070)
                     /locus_tag="VBC23_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842152.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            complement(2607..3467)
                     /locus_tag="VBC23_000172"
     CDS             complement(2607..3467)
                     /locus_tag="VBC23_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6842153.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(3560..4078)
                     /locus_tag="VBC23_000173"
     CDS             complement(3560..4078)
                     /locus_tag="VBC23_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6842154.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(4075..4830)
                     /gene="rlmB"
                     /locus_tag="VBC23_000174"
     CDS             complement(4075..4830)
                     /gene="rlmB"
                     /locus_tag="VBC23_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6842155.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4933..5319)
                     /locus_tag="VBC23_000175"
     CDS             complement(4933..5319)
                     /locus_tag="VBC23_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6842156.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(5312..6655)
                     /locus_tag="VBC23_000176"
     CDS             complement(5312..6655)
                     /locus_tag="VBC23_000176"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6842157.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(6652..6834)
                     /locus_tag="VBC23_000177"
     CDS             complement(6652..6834)
                     /locus_tag="VBC23_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842158.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            complement(6844..7428)
                     /gene="cysE"
                     /locus_tag="VBC23_000178"
     CDS             complement(6844..7428)
                     /gene="cysE"
                     /locus_tag="VBC23_000178"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6842159.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(7437..8189)
                     /locus_tag="VBC23_000179"
     CDS             complement(7437..8189)
                     /locus_tag="VBC23_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6842160.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(8191..10320)
                     /gene="pnp"
                     /locus_tag="VBC23_000180"
     CDS             complement(8191..10320)
                     /gene="pnp"
                     /locus_tag="VBC23_000180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6842161.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(10700..10969)
                     /gene="rpsO"
                     /locus_tag="VBC23_000181"
     CDS             complement(10700..10969)
                     /gene="rpsO"
                     /locus_tag="VBC23_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6842162.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(11057..12316)
                     /locus_tag="VBC23_000182"
     CDS             complement(11057..12316)
                     /locus_tag="VBC23_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6842163.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMSSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(12425..13354)
                     /locus_tag="VBC23_000183"
     CDS             complement(12425..13354)
                     /locus_tag="VBC23_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6842164.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(13351..14208)
                     /locus_tag="VBC23_000184"
     CDS             complement(13351..14208)
                     /locus_tag="VBC23_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6842165.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(14211..15566)
                     /locus_tag="VBC23_000185"
     CDS             complement(14211..15566)
                     /locus_tag="VBC23_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6842166.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(15579..15863)
                     /locus_tag="VBC23_000186"
     CDS             complement(15579..15863)
                     /locus_tag="VBC23_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6842167.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(15866..17902)
                     /locus_tag="VBC23_000187"
     CDS             complement(15866..17902)
                     /locus_tag="VBC23_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6842168.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(18118..19743)
                     /gene="treC"
                     /locus_tag="VBC23_000188"
     CDS             complement(18118..19743)
                     /gene="treC"
                     /locus_tag="VBC23_000188"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6842169.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(19965..21995)
                     /gene="treP"
                     /locus_tag="VBC23_000189"
     CDS             complement(19965..21995)
                     /gene="treP"
                     /locus_tag="VBC23_000189"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6842170.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAPKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(22277..22903)
                     /locus_tag="VBC23_000190"
     CDS             complement(22277..22903)
                     /locus_tag="VBC23_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842171.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(22887..23690)
                     /locus_tag="VBC23_000191"
     CDS             complement(22887..23690)
                     /locus_tag="VBC23_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842172.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(23702..24523)
                     /locus_tag="VBC23_000192"
     CDS             complement(23702..24523)
                     /locus_tag="VBC23_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842173.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(24520..25497)
                     /locus_tag="VBC23_000193"
     CDS             complement(24520..25497)
                     /locus_tag="VBC23_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842174.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(25610..27238)
                     /locus_tag="VBC23_000194"
     CDS             complement(25610..27238)
                     /locus_tag="VBC23_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6842175.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(27481..28209)
                     /gene="lrgB"
                     /locus_tag="VBC23_000195"
     CDS             complement(27481..28209)
                     /gene="lrgB"
                     /locus_tag="VBC23_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6842176.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(28211..28666)
                     /locus_tag="VBC23_000196"
     CDS             complement(28211..28666)
                     /locus_tag="VBC23_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6842177.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(28836..29576)
                     /locus_tag="VBC23_000197"
     CDS             complement(28836..29576)
                     /locus_tag="VBC23_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6842178.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(29557..31302)
                     /locus_tag="VBC23_000198"
     CDS             complement(29557..31302)
                     /locus_tag="VBC23_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO6842179.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(31329..31973)
                     /locus_tag="VBC23_000199"
     CDS             complement(31329..31973)
                     /locus_tag="VBC23_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6842180.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(32097..32492)
                     /locus_tag="VBC23_000200"
     CDS             complement(32097..32492)
                     /locus_tag="VBC23_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6842181.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            32573..33289
                     /locus_tag="VBC23_000201"
     CDS             32573..33289
                     /locus_tag="VBC23_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6842182.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(33343..33969)
                     /locus_tag="VBC23_000202"
     CDS             complement(33343..33969)
                     /locus_tag="VBC23_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6842183.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(34002..34325)
                     /locus_tag="VBC23_000203"
     CDS             complement(34002..34325)
                     /locus_tag="VBC23_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6842184.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(34322..34606)
                     /locus_tag="VBC23_000204"
     CDS             complement(34322..34606)
                     /locus_tag="VBC23_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6842185.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            34767..35006
                     /locus_tag="VBC23_000205"
     CDS             34767..35006
                     /locus_tag="VBC23_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842186.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            35191..36258
                     /gene="pepA"
                     /locus_tag="VBC23_000206"
     CDS             35191..36258
                     /gene="pepA"
                     /locus_tag="VBC23_000206"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6842187.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            36328..37098
                     /locus_tag="VBC23_000207"
     CDS             36328..37098
                     /locus_tag="VBC23_000207"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6842188.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(37119..37784)
                     /locus_tag="VBC23_000208"
     CDS             complement(37119..37784)
                     /locus_tag="VBC23_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6842189.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(37853..38308)
                     /locus_tag="VBC23_000209"
     CDS             complement(37853..38308)
                     /locus_tag="VBC23_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842190.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRFLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
     gene            complement(38349..38486)
                     /locus_tag="VBC23_000210"
     CDS             complement(38349..38486)
                     /locus_tag="VBC23_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842191.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(38545..38751)
                     /locus_tag="VBC23_000211"
     CDS             complement(38545..38751)
                     /locus_tag="VBC23_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842192.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(38902..40095)
                     /locus_tag="VBC23_000212"
     CDS             complement(38902..40095)
                     /locus_tag="VBC23_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6842193.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(40127..41101)
                     /locus_tag="VBC23_000213"
     CDS             complement(40127..41101)
                     /locus_tag="VBC23_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6842194.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(41216..41587)
                     /locus_tag="VBC23_000214"
     CDS             complement(41216..41587)
                     /locus_tag="VBC23_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO6842195.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKIDTLQVPLKSNDNKASHKH"
     gene            complement(41565..41957)
                     /locus_tag="VBC23_000215"
     CDS             complement(41565..41957)
                     /locus_tag="VBC23_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6842196.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(42018..43109)
                     /locus_tag="VBC23_000216"
     CDS             complement(42018..43109)
                     /locus_tag="VBC23_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6842197.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(42994..43926)
                     /gene="tadA"
                     /locus_tag="VBC23_000217"
                     /pseudo
     CDS             complement(42994..43926)
                     /gene="tadA"
                     /locus_tag="VBC23_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(44098..44463)
                     /locus_tag="VBC23_000218"
     CDS             complement(44098..44463)
                     /locus_tag="VBC23_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6842198.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(44577..48227)
                     /gene="rpoC"
                     /locus_tag="VBC23_000219"
     CDS             complement(44577..48227)
                     /gene="rpoC"
                     /locus_tag="VBC23_000219"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6842199.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(48344..51919)
                     /gene="rpoB"
                     /locus_tag="VBC23_000220"
     CDS             complement(48344..51919)
                     /gene="rpoB"
                     /locus_tag="VBC23_000220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6842200.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(52443..54740)
                     /locus_tag="VBC23_000221"
     CDS             complement(52443..54740)
                     /locus_tag="VBC23_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6842201.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            54851..56110
                     /locus_tag="VBC23_000222"
     CDS             54851..56110
                     /locus_tag="VBC23_000222"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6842202.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
     gene            56688..57718
                     /locus_tag="VBC23_000223"
                     /pseudo
     CDS             56688..57718
                     /locus_tag="VBC23_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            complement(57929..58741)
                     /locus_tag="VBC23_000224"
     CDS             complement(57929..58741)
                     /locus_tag="VBC23_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6842203.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(58731..59441)
                     /locus_tag="VBC23_000225"
     CDS             complement(58731..59441)
                     /locus_tag="VBC23_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6842204.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(59444..59887)
                     /locus_tag="VBC23_000226"
     CDS             complement(59444..59887)
                     /locus_tag="VBC23_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6842205.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
BASE COUNT        19349 a        12113 c         9546 g        18917 t
ORIGIN      
        1 aagtatgaat aatgcaacta ttagtagtat tatttgggta ctctttttca tttttactcc
       61 tttatgaaac aattacatca tctagctata atatatcaac ttatgaattt gaataagcca
      121 attatttatt aatttgtgag gcaatatctg agtctgtttg ttcgacaaca tccgcaactt
      181 tcaataattg ttgatttata tcttctaata attgtgcaaa ttgtgtgatt tttggagata
      241 attcattgaa ttgggcttca aagctatcaa atgctgtacc atcccaattt tcatcaataa
      301 cagcttgttc ttgagtcaaa actgttaaca catctgtaat tgattgtgat cctgttgtat
      361 atttttgtgc tgaaatacgg agttcttcag gtgttagttt aatttgtgac atcatttgct
      421 ccctattttt ttaactgcag tatagcatat ttagatgttc cttacacaga taaaactcat
      481 aataggttaa tctattaaaa atacaaacag cgatttatat tacatctact ttactttcaa
      541 aacggaaagg ttatcaaaag attttaaaaa aacaccctac aatatcttgc aaagtgcttt
      601 gaaacgttga tataacagta tattaacatt ttgagaattg tgatgcttta cgggcttttt
      661 taagacctgg ttttctctga caactgtaat atatggtgtt atatagtata aactagctaa
      721 tactcatcaa tttacatcca aaaagtgtac tatgtattgc aatatattcg cgaaggggtt
      781 atcaggttat caaaagtaaa attgacaata aaaaaacacc ttgcaattgc ttgcaaagtg
      841 ctttgaaacg ttgatataac agtatattaa cgttttgaaa attgtgatgc tttacgtgct
      901 tttttaagac ctggtttttt acgttcaacc atacgtgcgt cacgtgtaag aagtccagca
      961 cgtttcaatg aatcgcggaa atctgggtca acttctaaaa gtgcacgtga gataccatga
     1021 cggatcgcac ctgattgacc tgcatatcca ccacctacaa cgttaacaaa aacatcgtat
     1081 gaaccttgag ttgaagtaac tgcgaaaggt tggttgataa caagacgaag gtctgcgtgt
     1141 gggatgtact cttctacatc ttttttattg attgtaattt taccagtacc tggaaccaaa
     1201 cgaacgcgtg caacagcgtt tttacggcga ccagtacctg catattgtgc ttgtgccatt
     1261 atataatgct cctttcctct tagataagtc ctgagatatc aagtacttct ggttgttgtg
     1321 cagcatgtgt atgctcaccg ccaacaaata ctttcaattt cataccttgt gcacgtccaa
     1381 gagtgttatg tggaagcatg cctttaactg atttttcaat caaacgaaca gcatttttag
     1441 aacgaagttc acctgctgag atttgtttca aaccacctgg atacattgag tgagtgtagt
     1501 agattttatc tgatgctttt ttaccagtta atttaacttt ttcagcattg ataacaatca
     1561 caaagtcacc tgtatcagtg tgtggtgtga aagttggttt gttttttccg cgaagtacgc
     1621 tagcaactac tgcagaaaga cgtccaagag gtacatctgt tgcgtcaaca acgtaccatt
     1681 tgcgttcaac ttggcctggt ttagccatga aagttgtttt gttcatgatt tctcctatac
     1741 gaattcgttt ttgtttacag gtgtggatgt tccggtccac gagagtattt gaaaggttcc
     1801 ggggccttac aaatggggta aacaataccg cttataatca tatcaaaata aggtagcatt
     1861 cgtcaagtta tttcttttat tttatcaatt ttcatcccaa aataaatcat ctacagtttt
     1921 attcaaagct tgacacaatt taatcgctta attatagcct cctttttcaa tggcggctat
     1981 tgtctgacat gatacaccga ccttttcagc tagagactgt tgtaataaat ccatactagc
     2041 tctagctgtc tttaagcgta aatttttcat attactatct gtccttatac ttaaggtagt
     2101 atactgaagt tcccatagct atcaatgata ttcctataac tgctaaaatt gcatcatttc
     2161 cccttgaaaa ataagtagca atatcaactt cctagtaaaa gcatcaataa aatagttatt
     2221 cctatgaggg caaatgcgcc agcaacaaaa acattttcca aaatctttca tcaacaaacg
     2281 gagagactct ttttagatta gaagtcgtaa caaaatagaa ataccactaa ataaagttaa
     2341 tattattata aaatcaggtg acacgcttcc ctttattagc tttccaaaaa cactttcaag
     2401 ccataagata aaaaacatcg tctaaaaaca tttttgataa ctttgtaaaa ccaattgttg
     2461 ttgtcgttca tcatattgag gatagttctt attttatttt tcataatatg tctcctacta
     2521 ttttatagtt tcatagcatc atataaaata cattttataa aatgtatact aaaagaacat
     2581 aacgtactgt tatgcccttt gatttgttat cggttttctt cccctaaaga gaatagagct
     2641 aaagcacctg agcccacatg agctgaaata actggcccta atggacgaat aataacttct
     2701 tcaatacgct catctgctaa taactgttct ttcataactt gagctgaatc tttctctcca
     2761 ctataagcaa taattaaagt gctataagat agtgtttttg ttgcctgagt cacaatctct
     2821 ttaatacctt ttttacggcc ccttgtcttg gcaaatggta ctaatttacc ctcactatct
     2881 aacttaagta atggtttaat cttagcaaca cttccaatga tagctgcacc ttttgataag
     2941 cgaccactac gcattaaatg gttgagatcg tcaactaaaa agtatgtgtt taatttaggg
     3001 agaagtgatt caattttttg tttcacttct tctaaagact taccttcttg tcgctcttta
     3061 gttgctaaca tcgctaagac accctcacca catgaagctg ccattgtatc cacaatctca
     3121 atttgagcat caggatattt gtctaagacc atctcacgcg caatagttgc actctggtaa
     3181 gtccctgata aatgtgaaga tagcgctaaa tataacaagg catggtcatt ttctgcatag
     3241 gttgaaaaaa cttcttcaaa ctgaccaacg ttaatttggc ttgttgtagg ctttgctccc
     3301 tcttgcatac gctccaacaa gaagtcacta gtaatttttt cgtctccgac tgtctcataa
     3361 gtctttccat caagttcaat ggttaaacct ataatatcga cattgtgctc ttgagcccac
     3421 ttttcatcta aatccgatgt cgaatccgtt agtattttaa aagtcattgt tacctcaatc
     3481 tattattttt atttgataat caaagtagtt ccaatattat atcaaaaaaa ttgttggctg
     3541 gctaataaag tgttagtttt tacataccat ctaaaaaatc cttgagtttt cctagttgtt
     3601 catcattcca cggccttaac tttggcgtac tcaaatcgat ttgactggac atcttatcca
     3661 aatctgattt tactgtcgct acacgctgtt ccaattctct agctgagaca cgtaaagcac
     3721 cctgagaaaa tatagtccat tgttcattta aatcactggt agcaacggag actaaattta
     3781 acacagattg attaagttct gccgctgctc gttcaatata gctatccgca gtttcgtcct
     3841 cctcagtaaa gataacggat attttgtatt ggtcataacg ttgacgtaca ccaggaacat
     3901 actgggcatc aaatacacaa ataatatcaa tgtgttcgaa atgtgcgtaa tgatttagct
     3961 ttctaagtaa tacttccctt gcctcttcta aacgattgct tttaaaaagc tgtcgagtat
     4021 ctttccaaaa agcaatcata ttgtagccat caacgagtaa aatagaatgt tttttcatag
     4081 gcggtttcta aatacttcat acatcaaaat agcagctgcg acactggcat ttaaactctg
     4141 aacatgacca ttcatgggaa ttgtaatcat ttcatctact tgctttttga tattatgaga
     4201 aatacctttt ccttcattac caataactag ggctaattta cctttggtat tccacttatg
     4261 cgatggtgtc ccattcatat cagtgccaaa tatccagaag tccttatctt ttaaagtatc
     4321 caaagtttga ctgagattag ttacccttgc gataggaaca tgttcaacag ctcctgtaga
     4381 tgtctttgaa accaccgggg tcactccaac agaacgatgt ttggggatga taatgcctgt
     4441 tacattggtt gcatcagctg tgcgtagaat agaacctagg ttatgagggt cggtcagacc
     4501 atcaaggatt aaaattagag gattttcttc attctctgcc tttgtcatta tctctgataa
     4561 atccgcatag gcaaattccg aaacttttag aacgaatcct tggtgaactc caccatttgt
     4621 catgtctgat aagatttttt taggagtcca ggaaatggat actttttttt cagtcgctag
     4681 tgctttgact ttatcaacgt tctttcctct taagtcatct tgcaagtata atttattacc
     4741 agtattagct cttaaactct ctgtcacagc atgtaagcca tagacaaggt cacttgattc
     4801 ttctttaaat tgtttatctt tcatagtcat attatatcat gaatgctata gcataaagct
     4861 ttttatagca atattttacc aacaaatcct aatgtctcta taaaaaagag aggatatagc
     4921 ctctcttttt gtttattttt caatggtttc aatacaccac tgtattagag tttccaagcg
     4981 ctttatctga ccagtcatat ccaagtaccc cataagagcc tcaaaacctg tagacatacg
     5041 ataggtaata atatcagcat ttttagcttt tgtatgacta ttcgcattac gtcctcgttt
     5101 ataaattaac tgctcttcat cagttaaaat attttcttct aacatggcat taattaacaa
     5161 tgcttgggca ttagcagaaa catattgggt tgctttccga tgtaactgat taggtttggt
     5221 aaaaccctgc ataattaaat ggcgtcggat atacaaagaa taaactgcat ctccttcaaa
     5281 agctaaagca ataccattaa tcaaacgaac atcaatcacg agtccacctc acgccatcct
     5341 tagtatctaa caatttaatt ccttgttttg ctaattcatc tctaatacga tcagcagttg
     5401 caaaatcacg attagctcgt gcttcttgac gttgttctat taaagattca atatcagcat
     5461 caagaacttc ctcttggaat acaataccaa aaatttctaa taattcagca agggtttcct
     5521 taacttttga agtatagtgt cctgaattaa tccattttgc catttcaaag ataacagtaa
     5581 taccgtttgc tgtattaaaa tcatcatcca tcgcttcttg aaatgctttc acaaattgtt
     5641 ctaattcttc atcattagca ttctcttgga ttggtaagtt gaaagtattt tttaaatatt
     5701 taaggttaac ttccgcatca tgaacggcct tttcagtgaa attcacgggt ttacgatact
     5761 gttgtgtcgc taagaaaaaa cgtattactt gtccgtcaac gctcttcaac atatcgtgaa
     5821 cagtaataaa gttaccaaga gatttagaca ttttctcgtt atcaacgtta acaaaaccat
     5881 tatgcatcca gtaatttgca aaggtcttac ctgttttagc ttcagattgt gcaatctcat
     5941 tcgtgtgatg tggaaattct aaatcagctc ctccaccatg gatatcaata gtatcaccaa
     6001 ggatctcagt tgccataaca gaacattcaa tatgccaccc tgggcgccct ttcccccaag
     6061 gactttccca tgagacttcc ccagatttag ctgatttcca aagtgcaaaa tctaaaggat
     6121 tctctttaat ctctccttcc ccatcaacac gaccacttgc accaatctct aaatcttcca
     6181 aagttttatt ggctaatttt gcataatgat gtgatttact tactctaaaa taaacatctc
     6241 cattagcttc ataggcaaat tccttatcaa ctaaaacttt aacaaaatca ataatctcat
     6301 ccatatagtc aataactcga ggattttttg tagcaggttt gacacctaat gcagcaacat
     6361 cttccataaa tgctgaaata aacttatctg aaaaggattt tgtatccata cctgcttcag
     6421 ctgcaccttt tataatttta tcatcaacat cagtaaagtt agaaatataa ttgacttggt
     6481 aaccacaata ctcaaaataa cgacgaatag tatcaaaagc tactacactg cgggcattcc
     6541 cgatgtgtat atagttataa actgttggcc cacagacgta catgttgact ttaccttcat
     6601 tgagaggtat aaaatcttgt aaactgcgag tcatagtgtc gtaaattttt atcatggttt
     6661 ttcctttttc tgtttttgat aagctttata caaacgaaca atatacatta aagcaaaggc
     6721 agcccaaata attcctaaga gagagttacc ttgtgcaaac tttaaatagc ttaaacaagc
     6781 tcctgctagt atacctaaaa ttgataatat ttgagttgtt acataatcct tcacaaatac
     6841 ctcctaaagt ttggacgaat aataactttc ttctcgatca tgttcaatac ttcggatttg
     6901 aagatcatcc ttttgaccat ggacacggac aactttagct ggaacaccaa ctactgttac
     6961 gtctgctggc acatctgcaa ggacaactgc tgcagcaccg actttagcat tttctccaac
     7021 ctcaattgga ccgatgattt gagaatgtgc tgaaattagc gcacctttac gtatggttgg
     7081 gtgtcgtttg cctttgtctt tacctgtgcc acccaatgta accccatggt atagcatagc
     7141 acctttctct acaatggcag tttctccaat gaccaatcct gaaccgtggt caataaaaac
     7201 cccttccgaa atcgtcgctc cgggatgaat ttcaatttgt gtccagaaac gccaaaattg
     7261 actgtgcata cgtgctaaca atttaaaatt atgattccat aaaaaatgag atagcctatg
     7321 agctgcaaga gcttttattc ctggataagt taaaataact tctagagaac tacgagcagc
     7381 tggatcttgt tctttgacaa tagctatact ttctttccac caccccatag ttttcctcaa
     7441 tcgttttcta catcagacca atcaactaaa acttgttgtt catcaaatgg attgatggct
     7501 actccaaagg tatcatcaat ctcattttct ccattacgcg gtttataaat atcgtcaatc
     7561 gtccaggcta aaattactcc ttgtgctttc ctaaaagcac caccaaaatc attatggtta
     7621 taccattttt caaaacttaa aaggtttgag aatactggaa cataactttt tccttcgggg
     7681 gtagacattg tagggaaaaa acggtcaaaa ctaccttctt cacgtggatg gacaaaggca
     7741 ggtaccaagt aggttttatc catgatatcg gcaacaatgt tatcttcatt taacaactga
     7801 ttcaagattg tggtgtaatt gttcataaat tgcaccatat cttcacagta gaaaatagtc
     7861 gagttcccaa aatcgccttc tttttttaaa ttatagacca atcctgctaa gccatgtgaa
     7921 atagcttcat caagaacgtc taaactacga cgactctccc aaaacatatc tcgagcactt
     7981 tcttgctcct ctttgaaatg attaaggtca gtaatatccg taaaaacagg aacaacttct
     8041 tgaccatcta cttgaatagc atagggttcc ttgcttgccc aaaccgtaga gtggtgcaag
     8101 gcattcacaa gacctatact atctaaaaaa ttatccggtg cattgataaa agcacgcaat
     8161 ctaatgtcta actcgtttgt acttgtcata ttaagattcc tttttaggat tgtcagcttt
     8221 tggaggacgt ggtaataatg ctttcataga ggcatcaact cgccctttat catcaatttt
     8281 gatgactttc acatcaactt cttcaccaat ttcaagaaca tcagctacat ttgctgtacg
     8341 tgtccatgca atttctgaaa tatgtaccaa tgcatctgtt ttatcaaaga ggttgacaaa
     8401 ggcaccgaat ttctcgatac gaactacttt agcatggtaa acttctccca cttttgcttc
     8461 acgtactaaa cttgcaataa tgtctttcgt acgatcaata gcagcctgat cactagagaa
     8521 gatagataca tttccttctt catcaatatc aatcttaaca cctgtttcag cgatgatttt
     8581 atcgatagtt tcgccgcctt taccgatgac aactttaatc ttatcaacat cgatttttat
     8641 catatcaatc ttaggtgccg ttggtgctaa ttgtggacgt gactctgcga tagctccatg
     8701 aaggacatca agaatttcaa agcgtgcttt tttagcttgc gcaagagctt cttccaaaat
     8761 ttgaggggta ataccttcaa ttttaatatc catttgaagt gctgtaatcc cttcacgtgt
     8821 acccgcaacc ttaaagtcca tatcgccaaa atggtcttct aatccttgaa tatcagtaag
     8881 gactgtgtag tttgttccat ctgatatgag tcccatggca ataccagcaa ccggagcttt
     8941 tataggcaca ccacctgcca taagagcaag cgttccagca catatagaag cctgagatga
     9001 tgaaccgttt gattctaaaa cttctgcaac tagtcggata gcatacggaa actcctccaa
     9061 gcttggaagg acttgttcta atgcacgctc tcctaaagca ccgtgaccaa tttcacgacg
     9121 ccctgcagct ccataacgtc ccgtctcacc aactgaatat tgtgggaagt tatagtggtg
     9181 cataaagcgt ttcttatact ctggtgttag cccatcaatg atttgtgctt cacccatagg
     9241 tgctaacgtt aatactgata atgcttgggt ttgtcctcgt gtaaagagac ctgatccatg
     9301 aacttgtggt aaaaaatcaa tcactgcatc taatggacga atctcatcca cacgacgtcc
     9361 atcaggacga accttatctt ctgtaatgag gcgacgaact tctgcatgtt ccatttgttc
     9421 taaaatttca gcaacatcac gcataatgcg atcatattct tcatgttcag cgtaacgcgc
     9481 ctcatattca cctataacta cttctttaac tgcttctgta gctgcttctc tagccttttt
     9541 ctcttcaact tgtactgcag cctgtaaagc tatgttatga gtagcaataa tttctgcttg
     9601 aagttctgga tcaacttgaa gaagttctac ttcggctttt tctttaccaa tagcagttac
     9661 aatctcttct tgaaaggcga ttaattcaca gacagcctca tgtcctttaa gaagagcctc
     9721 tagcatgatt tcttctgaaa gttctttcgc tccagattca accatgttaa tcgcttcttt
     9781 agtacctgca accgttaatt ctaacgcaga agcctcttgt tcttgtgctg tcggattaat
     9841 aataaaattg ccatcaacat aagcaacttg aactccagca attggcccat tgaaaggaat
     9901 atcggaaatc gataatgcta atgatgaacc aaacatagca gccattggtg cgctagcatt
     9961 ttcatcaaat gaaagaacag tattgatgac ctggacttcg ttacgaaatc cctcagcaaa
    10021 catcggacgg attggtctat caataagacg tgctgttaag gtagcatccg tacttggacg
    10081 cccttcacgt ttgttaaaac cacccggaaa tttcccagca gcatacattt tttcttcgta
    10141 attgacttga agtgggaaaa aatcacctgt tgacattttc ttagacataa ctgctgctgt
    10201 aagtacagta gaatctccat aacgaacgac aactgatcca tttgcttgtt tagccacttg
    10261 gccagtttca acaactaatt ttttaccagc aaaaatcatt tcaaagactt gttttgacat
    10321 attattctcc atattttttg tttacgcgtt tcctacaaag ttcaatctag ctgttctaaa
    10381 ctcaactttg cataaaacca cgtataagct ttattctaac ataattctat aaaattgagt
    10441 agcgaaaatc cgtgggggaa gctgtattat cattcattaa tgtaaacgta aaaagtctga
    10501 ttattaatac tagcaatcag accttgttac aatgattaaa ataaatttct agggttaatc
    10561 ttagcttgct taataaccct cttcataggt taaaacactt tcttactgtt ggccccctca
    10621 aagtacttac gttcatagta cgtttaaaac aaaaaacact agaaaatttt ctagcgtttt
    10681 tacttgactc tgttgtctat taacgacgaa gtccaagtga ttgaatcaat tcacggtaac
    10741 ggttaacatc tgtacgacgt aggtatgcta ataagttacg acggtgacca atttttttca
    10801 tcaatccacg gtaagtagcg tggtcttttt tgtgttgttt gatgtggtca ttaaggtggt
    10861 tgatttccca agtaagaact gctacttgaa cttcaactga tcctgtatca ccttcgtgac
    10921 gtgcatattg agcaatgatt tcattttttt tctcttttga gattgccata atatattctc
    10981 cttttattgc ttcatccgag tcttaggatt ggcactccta aaaccaagaa aaagttggtt
    11041 tgtcttatag accctcttat tttatcaaat tagaataaac tttgtcaaaa gtttttaata
    11101 gaaatatgga taaccaactg tctctaaagg aaaatccttc atagactaaa cgagactttg
    11161 catctttttg ccgaaagacc ttagcataag tagatgacat actagttggc ccacacataa
    11221 atatcgttgg ttgtccttca aaaacagact gactaaaatc aagtcttcct tggaggctac
    11281 tattatttaa gtgaagttta aaattaggat tggctttagc ataggattct aacatatctt
    11341 gataaatcaa attgtcttga tttgaaaatg tataaaagaa gtcaactctc ttagtcaaaa
    11401 tactattttc tctaataaat gaaatgaagg gagtgatgcc gatacctcct gcaatccaaa
    11461 cctgttcttt cttgtcttta tcaaataaca tatgaccata agctctatct aaggctattt
    11521 ttgtcccaac ttttagctgt ttgtaaatgg atttcgtata atctcctgaa gctttaacag
    11581 ttaaaaatat cactctatca tgaccacccg atatcgagaa aggatgagct gctgattcaa
    11641 agccggcttg ataaattttt aaaaaagtaa attgtccata gtcatatcga taaggacgct
    11701 tcattgctat ttcaatctca gttgtatcat ggttaagatg agtaaccttt tgaacgtacc
    11761 ctacacgacg aaaacgcatt ctagaatata ggaaaataat ataaaatcca gatataaccc
    11821 caatgacagc ataacctaat acgattaaac tgagtaaagt attccctagt atcctatctc
    11881 ctaaaatcag aaaagtatgt accaatccca aaatatagac taaataaaca aagcgatgaa
    11941 taaaacgcca aatttcatac tttaaaaaat ttccgaaata agctacaaaa acaatactta
    12001 gaaaaagata taatcccgca ctgccaatag tctttgcaaa ctcacttcca tgtccgcctt
    12061 gtacaagacc aattttgtgc aatagaagca aaatcattga aaatacagcc attaccttgt
    12121 ggtaaaaata catattttca ataccttgaa agtaactttc aagtatccta aatttggttg
    12181 ataagaggaa tgttagcgat aaacttgtca aagctaatcc tggtacaaca aattcactta
    12241 atcctgcatt ttgccatgct actagtgtca ccactatgct agataaaatt aaaacaatac
    12301 ctttccaagc tcgcataacg accacccctt tatttttagt aaggataaaa taccacaaga
    12361 tacttataca aaacttaaaa atttcagaaa ataacaaaaa ggcaactcca atgttgcctt
    12421 tttgttatga tttataaatt gatttgcaat gctctactat atctttaaca gcgagtccat
    12481 actcctctaa taaatattcc atctgaccta cctgaccaaa tcgctcatca atacccatac
    12541 gactaacagt agtgtctttc tccatactaa gccattcgca taatgcactt ccgataccgc
    12601 caatacggtt atgattctca atagttacaa tggattgatc tatcagtaat ggtttcagtt
    12661 cttctggaag gggcttaatt ttaaaaaggt cgataactga ggcctcaatc cctagttctt
    12721 tgaggtagtc ggctgcttct atcgctctag aaaccataat accacttgct acaagagcaa
    12781 tatcttttcc ctggcgtaat tgtataaaac cttttgaaaa atcttcgcat ccctcataaa
    12841 ctgcagtggg ggccttacga atagttctaa tgtattttag accatcaata cttaatgttt
    12901 gttttaaaat tgcttcaaac tgaatatcat cactaacctc aaaaatagtc gcctttggaa
    12961 ttaaccttag tagacctaac tcttcaaaag gcatatgagt tccgccattc atttctgcgc
    13021 taactcctgc atctgagcca ataatcgtag ctgataattg tgagtaacct aacgataaaa
    13081 atacttgatc aaagacacgc cttgacgcga aaggaccaaa agtatgaagg tatggcttgt
    13141 aacctttaat ggcgagccct gctgcaagac ctaccatctc agcctccata atacctagat
    13201 taatataacg ttttccaaat tctgatgcca gtacatttgt tgacattgaa ctcgacaaat
    13261 cggcttctaa aacggtaatt tgtttatttt cttgattagc ttgaaggagg aaatcacgat
    13321 agacaagcct catttctttg gttgaacatc tcattctacc acctccaaac tttctctcaa
    13381 gcttatcacc gctcgctcta acattgtttt ttgttgtaaa tctggtcgta aatgatgatt
    13441 agaagctaac tcctccaact ctttcacacc ttgtcccttg atactatcta aaacaatgca
    13501 ttttggtttg acactatttg aatcttgaaa agttttaata gctttgtcaa ttgcctcaat
    13561 gtcatctcct tttacacgta ctgcatcaaa tccaaaagct tcaaatttag caacaaagtc
    13621 tccaggatta caaatatcag ctgtcaaacc atctaattgt tttttattat catctacaaa
    13681 aacaattaaa tggtgcagtt gatgatgcgc agcaaattgt atagcctccc aacattgtcc
    13741 ttcatttaat tcaccatcac ctacaatagt ataagtataa tagcttgaat tctcaatctt
    13801 ttgagcataa gcaattcccg ttgcaatact aatgccttga cctaatgagc ctgtcgttac
    13861 atctatacca ggagttaaat tgcggtcagg atgcgaaggt agtttggtac cgtttgtatt
    13921 gagcgaatga aggaatgttt tatcaaaaaa accttttaaa tagagtgtac tatacaaggc
    13981 tggaccagca tgtccttttg ataagaccat ataattccga tcactttctt tgaatttttc
    14041 agggtttata tccataatat caccgtacag aatagccaaa gtctcaacaa ttgataaact
    14101 acccccatag tgaccgaatc ctaaatggtt tagcgtttct agtgtattta aacgaatctc
    14161 cgttgcaaat cgtcttagag atgaatatct ttcttctggt gatgtcatta ttaaccttcc
    14221 ttatgggttg attttccaac aaatgaaatg ccaaaaagtc caattaagat aactacaata
    14281 ccaataacaa tagctatcgc accgccgaca tgattaagag cgccaagaat gataccggtc
    14341 aaaccaaaat cagcatctga gaatgttgaa cctttgaaac caagtccacc tagtacaggc
    14401 atcaagaaaa tcggcaagaa actgatgagg ataccttgaa caaatgcacc aatagtcgct
    14461 cctctcacac caccagaagc attaccgata acacctgccg ttgccccaca gaagaaatga
    14521 ggaacgaccc ctggtaaaat gacagtagtg ccagtcacaa tcataactat catactaacc
    14581 aatccaccaa caaaacttga gataaaacca atcaatactg cattaggtgc ataagggtaa
    14641 acaattggac aatccaaggc tggttttgaa tttggaacca atttttctga gattccctta
    14701 aaggctggta caatttcacc taggataagg cgtacacctg ctaaaataac gaatacacct
    14761 gctgcaaatt gcccagctag ttggagggca taaacaagac cattgacacc gttacttatt
    14821 tcttttgcaa tataagcctc accagcaaac actgcaacga taaggtaaat aattgccatt
    14881 gagatagtaa tacttacggt actgtcacgt aaaaatgata aactctttgg aaatttaata
    14941 tcttctgttg attttgattt atctccaaca attccaccaa taaatccact caaccaatag
    15001 cctaaagaac caaagtgccc aagtgccacc ttatcattac ctgtcagttg aatcatgtat
    15061 ttttgcacaa atgcaggaga tacgctcata ataatcccta acgctagact tccaaaaagg
    15121 atgagcgaga acgaagtaaa gcctgctacg gcaaaaataa cagcaatcat acaagccata
    15181 tacaatgtat gatgtcctgt cagaaaaata tatttaaatt ttgtaaaacg agcaattaaa
    15241 atattaaaaa tcattcccgc taacataatc aaagcagttg ctgagccata cttcgtaaga
    15301 gctactgcta caatggcttc attattagga actaccccca ccaaatgaaa agcatgttca
    15361 aacatttttc caaatggatt caaggaattt tggactatcc ctgcacctcc agaaaccact
    15421 aagaagccaa caaatgtttt tattccacct tttacaatat caggaacacc ttttttctgc
    15481 agtactaaac cgataatggc tatcaaggcg actaaaatag ctggcgttct ggcaatatta
    15541 actaaaaaat ccaatagacc tttcatggca tcaacctcct agattaagcc tttttcttgg
    15601 caaatacctg tcactaattc tctcaattca tccatatcaa tgatactgtt taaaatacga
    15661 acatccccca ggtgaccagc tgaatcctcc aaatcacgac caacaatcca aacatctgct
    15721 gcgcttggat ctgctccacc taaatcataa tgttcaactt caacatcaga aacccccaaa
    15781 tccttcaaaa tagactcaat attcatttgt accataaaac ttgaacctag acctgatcca
    15841 catgctgttc caattcttaa catctttaat cctcctgttt tataatcatt tcaatatctt
    15901 gatacccacg tgaagctaac aaagctttaa cattattatt atcacgaagt attgttgtca
    15961 aatgcgatag agctttcaaa tgcgtttcat tatcaatagc tgctatgcat aataagagat
    16021 aaatttcctg tttggggtca tccaataaat aaataggctg ttccaaaaca agcattgaca
    16081 taccgacaga gtttacccca tcttctggtc tggcatgtgg aatagcaata cctttaccaa
    16141 gattgataaa aggaccaaac tcttcgacct tttcaatcat agcttctggg taactttccg
    16201 taatttttcc agaagctaac aagggttttg ccgctaaacg aattgcctct ttccaattca
    16261 tttgttcaga acttgtctga taagtttcct ctgttataag ttggtgcaac aagggcctca
    16321 catcctttct aaatatatct tgttttacca ttgtcctcaa agccaatttt aattcctctt
    16381 cacaatgaac atgagcgtat ttttttatag ttgctattaa ttgatcaagt tggaaatcgt
    16441 caaggcaagc tttgggaaaa tctgaaataa ccagttcttt gagctgttgt acttgctctg
    16501 ctgtcatcat cagcgaaact agataattag gtttttttgt ttctacaaat atagtagaaa
    16561 agaccatatc gtaagtttga ttgtcaatca gtttcagctg ctcaatttta gagacacgat
    16621 gaaaatgaat ttgaggaaat aatccacgca atttttcctt aatcactaag gatgaactaa
    16681 cgccgtttgg acagagtatc aaagctttat aagacattga ttgtgtatca cctgattgtc
    16741 taagatagcc tccaaagtga atcacgaagt aagaaatttc actatcgggt attaatccta
    16801 cttgctcttc caaaggacgt agtgcctttt taaccaatag aaataaatca ctgtaatgct
    16861 ctttaatatt ttgtgtataa ccactatcgc cagttaaacc atatttcaat cggaaataag
    16921 ctggaataat atgacgcttc agaccttgta ataactcttc tttattggaa aaattaagaa
    16981 gtgaaacagt ttccatctca tcaacgattg cttttgccaa tgcttcaaag aaatcatcat
    17041 ctttagttcc ctcaccttca aaacagccag ataagatcaa ggtaatataa tctttttctt
    17101 tctgatctaa tggtttagat aaggaaatat cctgacttaa attagtcaca ataatagtcg
    17161 ttaactcttt taattgttca gaaacaatag gagaaccctg caaaacacga tctactgacc
    17221 tttgataacg acacagtatt aaaatgataa aatataaaga tttctcaagc ctatcttgta
    17281 ttggagaaag ttgaaaagat tcatagaaat attctaccgt cttttctaac ttctcatatg
    17341 agagagcgaa tttccaagaa gacaagatat aattcaaaga ccaaaaacca atgggagatt
    17401 ctagtagttg agttatcatc tgaaaagcaa cttgatgctt atccatctca tctcctacta
    17461 gactatatcc ctttgctctt gtataagtga gtgagatacc tctttttgct aatttagaac
    17521 gtagcatctt gatatctgat aaagtagtat tcttacttac tttcaaaaaa tcttgataat
    17581 gcacattgga gacaaactct cttcggcaaa agctataaag gtaaatcaga catacccttt
    17641 cttcctgagt taggtaaaat tgctcagctt ctaataagga gacaatttta tcatgctctt
    17701 tttcatctcc taataactga taacgtccag cagagtgagc aatagctggt aggtgatttt
    17761 ttgctaaaaa actattgaga tagttaattt gctctatgat aacttttatt gaaaactttg
    17821 tttgcactgt taactcatac aaggaaagat ttttccgagc taaaaatacc aacattatca
    17881 caacagtctt attatcaacc atttgttttc ctttctattt ttattttata gtaaccgttt
    17941 acatttttct aggtcatttg agcccaatct ggcactacta tcaattgaat gaatttgggc
    18001 tattgttata gagtaaagaa aaaagaaaga aattgcaaaa tctagagcaa gattccttcc
    18061 cacaagcagc acttaaaaaa cctttactta attgaataag taaaggtttt ttaaatgtta
    18121 tgacactaag atagctaaag tttgataagg ttgcaaagta accgtttcat caagagtaac
    18181 atccttataa tttgatagaa gaacttgtcc ttggagataa ttctccggta attttatctt
    18241 aactttctca gcaaagaaat tatttaaaac aagaagtttt tccttatcaa gatggcgttc
    18301 aaaagcatat actttttcgt tatctttgaa agctgcttta taattcccgt cagctattat
    18361 tggcagttgt ttacgtagcc ttatcaactc ttgatagaaa gtaaatatca gccctgtttt
    18421 ctcctttgcg acattgattt ctttatatga ttttcctact ttcaaccatg gagcgccttc
    18481 agaaaaacct gcattcgtac tatcatccca ttgcattggt actcgagaat tgtctcgaga
    18541 tttcgctcga ataatgctaa atgcctcttc ttgtgacttg ccttcatcaa gcattatttg
    18601 gtaggcattc aaactctcaa tatccacata atcatccata gaactgtaat cgggatcaag
    18661 cattccgatt tcttcaccca tatatatata aggagtacca cgcgataagt ggatagatgc
    18721 tgctaacatg gttgctcctt catttcgaaa acgtttcacg tctacaaaac gatttaatgc
    18781 tcgcggttgg tcatgattat tatagaataa agcattccaa ccatttcctt cgctcattcc
    18841 ttcgccccaa ctatggaaaa ggtcacgtaa tgcaggaaaa tcaaaagcca taatggtcca
    18901 cttttgccca tctttataat ctactttgag gtggtgaaaa ttaaaagcca tggataactc
    18961 ttcacgttct ggtgcagtgt aaagaatgca atttgcaatt gtcgttgaag acatttcacc
    19021 aactgtcatg aaggaatcag cttgtccaaa actagcatta ttcaacatct tgagataatc
    19081 atgtgttatt ggacgatcag tataagctgg ttttccatca ttaattgggc agttctcaag
    19141 tatctcatct ttcccaatta aattaatcac atcaaatcga aaacctttaa cccctttatc
    19201 acgccaaaaa ttgacaactt taaaaagttc cttgcgaaca tcggcattgc gccaattaag
    19261 gtcagcttga gtgatatcaa aaagatgaag ataatacttc cctgtatcac caaatggtga
    19321 ccaagcattt ccaccaaatt tagataccca atctgttgga ttatcacgta agataaagaa
    19381 atcttgatag tagcgatctc ctgcaagtgc ttttttaaac cactcatgtt ctattgaaca
    19441 atgattaagc accatatcaa gcataaaatc tatacggtat tgacgtccca cctcaatcat
    19501 ttcttcaaaa tcgtccatcg ttccaaaatc agggttaatg gctgtataat cagagatatc
    19561 atatccatta tctcgttgtg gacttggata aaatgggttg agccaaacca tatcaatacc
    19621 aagttctgct aaatagggta atttttcgat aataccacga agatcaccaa ccccattacc
    19681 agttgtgtct ttgtatgatt ttggataaat ttgatagaca actttacgtt tatcaatcgt
    19741 catattctgc tcctttccca aaatgctgct gtatttagca gtctaactat aacatcaact
    19801 atcatcaaaa atataacatt tttacagcga tatttgttcc catgagtttt ttcttatgtt
    19861 caaaataatt caaggagcta atgtattaag cccctcaaac aattcctcaa attacctctc
    19921 attttataag agtgctcttt tttaaaaagg atacctaatt acttttaaat tttcttggca
    19981 accattaatg cttgtccacg ttttatctgt ttgggtaaat ctatcaattc ttctggtcgg
    20041 aattcttctt gattagtaat cagaatagga gtttccacaa tgtatccttc ttcttttatc
    20101 attggaatat cgaaagtaat gagtttatct cctactttta cattgtcacc ttgggcaaca
    20161 tgagaggtaa acccttttcc ttctaaatta acagtatcca taccaatatg aataagaaac
    20221 tcaacaccct ctgatgttaa taatccaata gcatgtttcg tagggaaaag aactgaaact
    20281 gtcgcatcga ccggtgaaac tagttctcca tcacttggat cgataaccac tcccttcccc
    20341 ataattcctt gtgaaaagac aggatcacta gcttgatcca gtggtttagc taaacctgat
    20401 aaagggctta ctacactaag ctttgtacct gagacgacag ctttttcttt tgctgatttt
    20461 gtttctgttg ttgaagcaat gacagcatca gatactaatt tttcctcttc agtttttgtc
    20521 aaaatacctg attttttaaa gaatagtgtc aaaaataaag gaataaagat agccactgcc
    20581 atacagataa agaaatatcc catatattta acattaattg acaggaagcc tggaagaccg
    20641 cctacaccaa tagagttagc ttggacatta aaagttgtcg ccaaaagacc tgccacactt
    20701 gaaccaatca taccagcaac aaatggataa atgtatttaa cgtttacacc aaaaagcgcc
    20761 ggctctgtaa caccaaggta ggctgagata gcagctggaa gtgatatttg agcctctttt
    20821 tcatcatgac gatgcataaa ataataagct agtacagctg aaccctgagc aatatttgaa
    20881 agagcaatca tcggccaaag gccagttgta tgtgtttttg tatctgctat gagttgagta
    20941 tcgatagcat ttgtcatgtg gtgcaaacca gtaataacaa atggggcata cagggcacca
    21001 aaaatagcac caaagagcca ttttacagga ccagtcaatc caataagaac gatagcagaa
    21061 atccatttac ctagtgtcca accaataggt ccaaggacag tatgagccaa gataatagct
    21121 ggaactaagg atagaaatgg cacaaaaatc attgaaacaa cttcaggaat atgttttctc
    21181 cagaagattt ctaagtaaga caggctcaaa ccagctaaca gagctggaat aacttgagct
    21241 tgataaccaa ttttttgtac tgtaaagtaa ccaaaattcc aagaccaatt tttagcaatg
    21301 tctgctgctg aagtactagc tacagaataa gcattaagta attgtggcga cactagacag
    21361 atacctagaa caataccgag gatttgagta gtccccatct tacgagtcac agaccataca
    21421 ataccaactg gtaagaaatg aaatatagct tcacctggta accataaaaa agagtctact
    21481 ccgctccaaa acgttgaaac atctactaga gtattccaaa ttggatgccc agaagagtct
    21541 acttgcctga caccatcaac aactttttga cctaaaaatt caaatggaac ggcatctaaa
    21601 atattacgaa aaccaaggat cagaccacca acaataatgg ctggaataat cggtgtaaat
    21661 atttcagcca gcattgtcaa cacacgctgt aaagggtttt gattttttgg ggcagccgat
    21721 tttgccgcct ctttagacac accctcaata ccagaaacgg ctacaaaagc attataaaaa
    21781 atagggacat cattaccaat aatgacttga aattgcccag cattggtaaa ggtacccttg
    21841 actgatggta actcctcaat tacctttacc ttagctttac tactgtcatt caaaacaaat
    21901 ctcatgcgtg tggcacaatg tgtcactgca ctaatatttt ctttaccccc aattgcctct
    21961 aacaaggctt tggcatcatg tttaaattgt tccatctgtt tatccttatc tatttaacgg
    22021 agagtctgtg actccaaggc agaaacttaa agaagactag cttacaatat aattgtaaac
    22081 gattacatat ccctatgcaa atccttagct tatcttttta aaaaatctat aaattataaa
    22141 ataattagtc aataataccc aaaacattag aatttctcac caacgttacc aaaatgttag
    22201 agtcatgagt aggtcagtca cccccaatac tctcttaata aaaaacacct actaattgat
    22261 atagtaggtg agttttttaa tctccattga aagatacgat acgattctct tcaatcatat
    22321 agcattggtt acaaagtttc tctaacatag cgaaatcatg actaataacg atgagaccta
    22381 aatttctatc ctttacaatc tccaacaggc ttttccatac actagcttgt gtaatactat
    22441 ccaacatagt agtcatttca tctgcaataa ggtatttagt ctctggatgt aatgaacgca
    22501 caatcgaaaa gcgttgtaat tcccctcccg agagttcact agggcgacga tttagccatt
    22561 tttcttgaat tccaaaagca tctagtaaat ctcgacttgg gtaataagct tcttctaaac
    22621 tttttttcat aggccataaa ggattcatcg ttttttcagg atgttgttga ataagttgta
    22681 caggtctaaa tgctttgtta ggcaaattac tgccatcaac taatacttca ccagatctag
    22741 gatgtaaaaa acctgcaagc actttggata aactagtttt tccacaacca ctttgtccaa
    22801 atatacctaa aacttgacca ggtgctacct ctaaattaat ctccttaaaa agccattgat
    22861 cttttttatg ataaaagcca agctttttag cctctaaggt catgagtaac tcctttcaaa
    22921 aattcttgct gtgggagaga gcgccataaa cttctagcaa attctgtttg taactgctct
    22981 ccatttccgc taaagaaact agctggagct gtttcaatag cttttccctc tttaaaaata
    23041 gtaatacgat cagcaatttg actagctgct acaatatcat gagtgataaa tataacgctt
    23101 attcctttat ctgcaaagga gcgtagttgg tctaaaacca tttgcagagc atctggatgt
    23161 aatccagggg tgggctcatc cgcaataatc aaagaaacct tatcactaat acacgttgta
    23221 aacaaaacac gtcggagcat tccgccagaa agttggaaag gatacaagtc accatcactt
    23281 tcttttaaac caaactgttg aaacaatcct tcttgagtag cctttgaatt ttctgagata
    23341 cctaagcgca cctgatgttt gactttcata gatggatcta aataattaac tgattgtgga
    23401 atcaacgtaa tatcttttcc tcgcaactgt ttaatgcgtt tagaatttag tgattgacca
    23461 cgataaatca tatctcctgt tacagatgca tttttaggaa gaatatccat aatagcatgt
    23521 gctaataaac tcttccccga accactagca cctataatag ctaataactc acctttttta
    23581 atttctaaat tcagcgcttg tatcggtgtt gattgaaatg gttttaaaaa tcttccgtat
    23641 tgagtgaagg tgatggagag gtctttaatg cttaataatg tttctgtcat atctctacct
    23701 cctaaaaatg atcagtttga gggtaaaaga gtttctttaa agattctccg atagtatcaa
    23761 atgcattgac aaccaaaata agataaagtc ctggaaagat aaccaaccac caatttccaa
    23821 gagagatatg cttagctgcc tctgacagaa tgataccaac cgaaggttgt tcggcagaga
    23881 gcccaaatcc taagaaagtc attgatgctt catgtaggat gacatgtgga aataatagga
    23941 taaaaccaat gaaaatttga gaagcaatca aaggcaggat atgatgcctc acaatataat
    24001 aaggcgtttt tcccatactt ttagaaagtt ggacaaattc tttattcttt agatgataga
    24061 cttcattgcg gataagcctt gctaaagaag gccaatgtgt aacagccgtt gcaatgatga
    24121 ccccttgagc acctttccca acaacaaaag aaatgagaat cataaaaatc aaatgaggca
    24181 taccaataaa caaatcaact aaccatgcta ttattttatc aataatgcta tttcctaaac
    24241 ctgcaagcac tccaaaaact gtcgccagaa tgacccccat aagggcacct aataagccga
    24301 cttgtagaga gaaataaagt cctttaatcg ttctgacaaa catatcccta cctaaaccat
    24361 ctgtcccaaa aagatggttt aatgaaggag caaggttccg taaagctgca ttggtttgca
    24421 agggagtact atggaaataa agatttaagg ctagaatact aagaatgaga gaaatggcga
    24481 tacccagttg ccataaaaca atagttcgac gtctcaatat catacttttc tccttaactg
    24541 tggattgatt atgctattta aaatatccgc aataagattg cccgcaaaaa caaataatgt
    24601 ccctatcatc acaatagcta gaagtagcgg tgtatcactt ttaagtcctg cttcagttag
    24661 ggtagaccct agtcctggat atgagaaaac ttgctcagca agaacggatc ctccaaacaa
    24721 ttctccaaaa taggaaaaat gcagtgtaat agctggtacg atagcatttc taagacaatg
    24781 atttttaaaa atttgccatt ccgtttcccc acgcgctctg gcaaataaga catattcact
    24841 agaaagtacc gacatcattt tagttctagt atgaagagtt acattggcaa tgcctagaat
    24901 acttagcgtg aaaacaggta aaataaggtg cttaatacga tcagctaacg taatatcttg
    24961 actcaaagta cctatcggag aagaaatacc aatcgggaac caccccagct ggacagaaaa
    25021 gattaataaa aaaatgagcc caatccaaaa agttggtact gatatctgaa ggtaagaaaa
    25081 ccaccttaca attcgatcaa gcaatttccc ttgatggaaa gctgataaag ttcctaagat
    25141 aaatccaata agacccgata agacccagga gagtcccata agtatgaaag aggcacctgc
    25201 ccgtgatcta ataatatcaa taacaggttg ccgataaact agtgaagtcc ctaaatgtcc
    25261 ctgtataaaa ttttttaacc aaataaaata ctggaccgga gctggcttat ccaagccata
    25321 gtggtgagca atcgctttgt actgaacagg ggttagtgac gtgtcataat tgacacttgc
    25381 catgactgga tcaaccggtg attgttttag taaaataaag gtcaaaattg atactccaaa
    25441 aacgagtgac aaacacctaa gtatcttcca gataactaca gtggtaatat gtttcacaga
    25501 taaatctcct taattgttaa aaaaggagtc gaggaaacca ttcacaccat aaggtgtaca
    25561 ttgttttctc tgactccaaa cttaaagtag cctacattaa ctaaattagt tacttagcag
    25621 attcatccca agtccattca gcaatgttag tcaataatga ccaatcatga ccatgactat
    25681 ggacaccttg tttacctaca ttgatacgtt tatcaccaat ataagtatgg ttaagactca
    25741 ccaaccatac atttggtaaa tctccaagag tggaagcacc tgttttgcca tcccactgag
    25801 ctaacttcca atatttgtta gctttatcaa ggtcgggaga tgtcattgct ttgtcaaggt
    25861 acttagtcac agtaggattg ttataaaaag taatattggt ccaaccttta ccagctaaac
    25921 ttgggtaatg tgattcataa aattgctgcg catgatgacg tcctcccgca taaagtaagg
    25981 ctgaatcatg tgacttagtt gccatttcat cccagttact agccttgagt ttaatagtaa
    26041 tacctaaggc tttagcttgc tccgctactt caacggctaa gtttgctcgt aattgatcat
    26101 tagtagggta gtaaaggtca aattccgctt taagatttcc ttttttgcgg ctaccgtctg
    26161 cttgttcttt ccatccagct tttgtcaaaa gttgcttagc tttagctact ttattatctt
    26221 taattgctgt ttttggattc cagaatggtg ttctatcaat aattgaataa gctggtttac
    26281 cataaccatt taaaacagta tccagaactt tttgtctatt taaaccaata gtcaaggctt
    26341 ttctgattgc tggatcacta gtgacatcat ttcctactgg ataaccgtct ggtgaatttt
    26401 tcacaacacc tttcttcaca taaggtagcg ataacccacg aacatcattt gaagcaatat
    26461 ctagaagacg tgttcctttg actttcttgc tagcaagctc tggcgttgcg taaatcatgt
    26521 caacatcacc agattctaaa gcagctagtg ctgtattttc atcaagtaat acccaagtcc
    26581 actttttaaa ataaggcttt ttaccgtgcc aatatggatt tcgaacgaag attgcttgtt
    26641 ctccagcctt atattctttt accatgtaag gtcctgaacc gataggattg ctcttatact
    26701 tatcattgta atgtttttta gggacgattg ggatttctgt caactgtgct gtaaatgtcg
    26761 aatgcgcctc agtcaaatga atatttacct ggtttttccc aactacttca acattcttaa
    26821 taaaggtcaa atcccaagct tttccatctg ctttcaacat atcataagta aacttaacat
    26881 cgtcagcagt aacaggctca ccattggaga atttaaaatc atcatttaag tcgaacgacc
    26941 acgttaagcc atccttagag attttatatt ttttagcaag ctctcctttt atatctagtt
    27001 caggagaacg tttcaatagg gtactatgag taatattacc ttcattatgg attccataac
    27061 ggtcctttgg atcgaattca tgaggaagct ttgcccccat agagacaacc aattcatctt
    27121 tgggtcgttg ttttcgtgtt cgctcctttg tttgtgaatt ttgattttga caagctacta
    27181 aaaagatact cgccagaaac aaagcaacaa aagagatgta cttcaaatat ttagtcacta
    27241 ttaaacctcc ttttttaatt gataaataat atttttgaca acttttttca ttataacaag
    27301 aaaaaagtag gagaacaatt acttaactgg ttaattagaa aatttgttat tttatcatta
    27361 ataacttaaa gttaggtaaa acacaattct agactctagc tcataaacca tgttaatcca
    27421 atgtatttta atagacatca taaaaaggct gaataaattc agccttaacc acgctattag
    27481 ttatagcaaa ccaatcaaat gactaaagat aggaataacc aaatcaacta ccaaaccgat
    27541 aaccaccacc gctatagctg ccatcgcacc ttgcagttct cccaattcca atgcaaaggc
    27601 tgctccgact gtatgaccag aggtccctag acctaatcct gcaccaatct tactatcatt
    27661 caaatggaaa aatgacacta attttttacc taatgcataa ataataactg catttaagat
    27721 acacgccatt gctgtcacag ctgtgttccc tccaattgct gctgttattg gaagagcaat
    27781 tgctgttgtt gctgcttgtg gcaacattga agcaattccg acttgactaa gcccaaccat
    27841 tttagaaata gcgacgataa gcataaggga aacaatcata ccgattacta aactgctgag
    27901 aatttctacc caatattttt taacaacgtc atttttctta tagagaggaa ctgcaaaagc
    27961 aattgttgct ggatttaaaa accaaaatat taaatcgcca cctggtttat aagcttgtgt
    28021 ataaaatgtc ttaacatcgg ttcctaaacc ttttgacata agccaaagaa tgacaatacc
    28081 actaaccatt gcaaagaaaa gaggctgcaa aaggaagaaa cctttactct tcttaaataa
    28141 atgttgtcct atcgtataga gtattaaaga aaagcaaata ccaaagatgg gtgtttttaa
    28201 gagttccata ttatttttcc tctactttct tactatagtg gttaggtaat atctttttaa
    28261 ataaatctgt gacactgagt tctctgatag caagtattaa tcgtgcaacg tatgctacca
    28321 ctactaacat aacaacagtt gagattgtaa taacagcaac tagttgcaag ccttcagctt
    28381 ttaggatatc aaggttcgct gctactgata ttcctgaggg gacaaacata aaaccaatca
    28441 tgcttatcat caaagcccca aaagagtcaa cccactctac cttgataatt ttagcagtta
    28501 gcaatacgta cattagcacc aaaccaataa ccgtcgttgg gattggtagt gatttcggta
    28561 ataacattga aatcatttgt gaaactaata gaatagctgc atatattgac atttgaataa
    28621 acattggagc ttcttttgtt ttggtttctt tactggtgtg tttcatagtc actacccctt
    28681 ccttttatta caatgtttta acttgtttta ttgatagcta tattgtactt caaaataaat
    28741 aaaacgtttt cattttcagc ctcagttacc aaaaaaagaa gccgaattgc aaattcaagc
    28801 ttctaaacaa caaagggaag ttcgcaaaag cacttttaca tggttaaatg ctcatttagg
    28861 tcttttaaat acgatcgacc tactggaaat ttctctccat tagacataat tagtaacaaa
    28921 gtatgattaa accaaggttg aatttcctca atcatctcta gattaatcac tgtgtttcga
    28981 tgaatctgga gaaaatatgg tgctacagcg cgtgacttaa accaattgag tgttttccga
    29041 atagtgtaag ttttctgaac tgtactaaca gttaattcgc catcttcaac actagctgca
    29101 acgatatctt gcattttaac aacaacactg cgatctgata atgtcaaggt taacaattca
    29161 gcactggctt ttttaggtat tgcttgctct acatctgaag cagtggtgag ttggctcaat
    29221 ttcttcactt ttgatagagc catatcaacg cgctgttgtt caaacggttt catgatataa
    29281 tcaacagcat tactttcaaa agctttcaca gcgtagttat cataagctgt cgcaaaaaca
    29341 acaagtgggg gatgtgcaag ttggctcaac tgattagcca aagtaaagcc attttcttca
    29401 ctaagcgaaa tatctaaaaa aattaaatca atttgctgtc taaataagat tttttcggct
    29461 tcactgatat cctcagcctg aaaaatctca gggttgtcaa cctcctggct atgttcaact
    29521 aaaaacgata attcttgtct ggcaaacatt tcatcatcaa gaattaaaat tttcatgctc
    29581 atcctccctt attctattag gtattcgata ccaaactttt gtaccattct tgtcgctcga
    29641 aaaatgaagg caacttacac taccatataa taaattcagc ctgttattta gattaactag
    29701 agcagtacct gtccccttac tctctgcaac tgtttcttga cctaatttat caatgatagt
    29761 atctgagatt ccttgtccat tgtcactaac agaaacacaa taataatgac catctggctt
    29821 tatttgaacc aatatatggt tgtccgtctt acgttctttg aaagcatgtc gaactgcatt
    29881 ctctaccagt acctgtaaac caaaaggcgg taacttcatt ttttctggtg cactaatatc
    29941 ataagataac tgatatttat cagggaaacg taatttttca acattcatat aagcatccac
    30001 atgtgatttt tcttgctcaa gcgttacctc acgatcctga ccaccttgca aacttgttct
    30061 aaaaaaagta cttaactgca tcagtgcata acgtgcttta tcagaatcaa tacggattaa
    30121 tgcactaatt gtgttaatgg cattaaagaa gaaatgaggg ttgatttgtg cttgtaaagc
    30181 ctttatctct gccatactgg ctaacttatt ttgttcctct gttatcccca ttgccagttg
    30241 tcctgaaaat atttgcgcta aaccaaggac taggttttcc tccacctcag acattgtctt
    30301 atctcctgca aagtacattt ttaaggcaca cacagtttta tcatttattt ttagaggaac
    30361 tacaatagca gaatttaact gacagttgtg atctggacaa gaaatcgccg ctttatcttg
    30421 cgcaattctt ggttcgccat caaaaataac acttttagat aagtctgttt tgaccggttg
    30481 tcctgcaata tggtgatcat ggccaacacc aatatgagct aatacgtttg accgatctgt
    30541 taatcccaca gcatcaaagt tagtatgcct ctttataatt tcgcaaacgc tcctagcaga
    30601 ttgcggtgtc aaaccttgtc taaggtaggg cagagtctgt cgagtcaatt caagaacatc
    30661 tctcgtttga actgcgcgta actgactttc atttgacaaa taagttttca aaatcgcaag
    30721 gaaaagtgtg gaacctaaac tatttaaaat catcattgga atgacaatca ttttgacaag
    30781 ttcccatcct gtaaaaatgc caacaaatag catctggata ctttcggcaa taatactaat
    30841 taaaataact tggcttgttg aagggtagag atggttttcc ttaagcttat caccaatctt
    30901 tccgctaaca atgccgacta gaactgaact gacaatatag aaagaacctg aaaagcttcc
    30961 ttgaaaaaag cgatgaactc ctccaataaa accaacaatt gatccaacca gaggtccacc
    31021 aaccaaactt gccgttgtaa taactaaagt ccttgtatta gcaagtgagt cagaatggga
    31081 aatcgttgtt agaaaagggc gctcgaccaa acttcgatcc ccttttattt caattcctgt
    31141 tatattagat ataataacaa acaagccgaa aatgatgaca aggactaccg tttcacgttt
    31201 agaccgctct tcaattaact gtctgaaata actattattt accaataaaa aggctaaaat
    31261 cataataatt cctagccttt ggaataacaa caccatcaac atatgcttat cctcctcaag
    31321 ataccatttt actccggaag ataaatatag atatcttgat aatctgaaat aaccttgtct
    31381 gccattgata aatcttgcgg tggataatca ggattggcaa agccaaagca atacattcct
    31441 gctgccttag cagctaaact cccatttctt gtatcttcta taactataca aactttaggg
    31501 tctacatcta aaagctctgc agctctaaga aaaacatctg gtgctggttt agaagacgat
    31561 acatcttctc ctgtaaccat gtattcaaag cattctgtca cacccaactc ttttagatta
    31621 cgcttaatat ctaccattgg agatgaggac gcaactgcta agcgatagcc atgttggtgt
    31681 aaccaatgaa tcagttgctg agctccttta atgggcctaa caccatcacg cgtcacaatc
    31741 gcttgtcgac gtctgttcat ctcagcaata tactctttta ctgttttcgg taagccaaac
    31801 tcctctttca tagcttgcca catgaattca aaagtagtcc ccatgtactg gtattgatac
    31861 gagacgtccg tatcaatacc ctcctcacgt aacatttctg tcttattatc taaaaacgtg
    31921 tactcggaat caacaataac gccgtccata tcaaaaataa tgactttttc catcttaaac
    31981 ctgctttcta tcccctttat tgtaatcgtt tgcctttcta ttttcaataa aaaaaggttg
    32041 ctttctgtgg aaagcaacca gtatatttag aaatgatatt ttgagagtga ttttatttaa
    32101 aaggggagct cctcttcttc caatactaaa tctgacaaat caccagaaac attattttca
    32161 cgcatagcac gttgggcacg gctttctaac aactgaaatg atgatgctaa tacttcagtg
    32221 atatagtgcg tttgaccatc cttttcgtac ttgcgcgtac gcaattcacc atctattgaa
    32281 attaaagagc cctttgtccc atagctcgca agggtttccg ctagacgacc ccacataacc
    32341 acattaataa aatctgcttc acgctcacca ttacttcctt taaagcgtct attaacagca
    32401 acagttgcac gcgttactga cttgtcagtt ggtgttttta ccatctcagg ctttgctgtt
    32461 agacgcccaa tcataataac tttattatac attgattacc tcctcactat tattattcga
    32521 aaataaggaa gaaaaatgct aaaatatatg aaattgttct gggagaatta agatgacaaa
    32581 tgtaactagt tataaagaaa tgctggctaa accttgggga aaaatacaat atgaaataac
    32641 ttttgctcaa ttaagccata tcaaaaatca gaacgtttta gattttggag ctggtttttg
    32701 tctcaccgca caacatctag caaaagaaaa caatgtaact gctattgaac ccaatcctaa
    32761 actgctttac gataatcaaa gtgacaatat ttataaaatc ctaggttcct acgaagcatt
    32821 aagagctttg cctgatcaaa gttttgatac tattatttgt cataatgttc ttgaatatat
    32881 agacaaacat aatcatcctg cttattttga cgaattttct cgacttctaa aacctaatgg
    32941 agaactatct ctcatcaaac ataatataat tggtaaaata ttacaatcag ttatttttag
    33001 caatgatacc tccactgcga tggaactctt aacaggagaa gcaaacttta aaagtgccag
    33061 ttttgaccag ggaaatattt ataccttaga agaactaaaa caaaatacaa atttattagt
    33121 tgaacgttat cagggtattc gtaccttcta ttcattacaa ccaaaccatt ttaaaactga
    33181 aactgattgg ctaaacaaaa tgcttgctat cgagctaagt gttgctgaca aagctcctta
    33241 taaagatatt gcttttttgc aacacatcac acttaaaaag tcattgtaac ttcttaagtt
    33301 tacaactaaa aatcaactta gctgaagcta agttgatttt tactaatttt tccaatgttt
    33361 attggcgtcg aaagactctc caacaattac ctgatcagat aattcaatga tacctctgac
    33421 ttgaggagca tttggaagag ctaactcgcg agcactacat aacattccaa agctatcttc
    33481 tccacgaagt tttcctggga aaataaggct accatttggc atcatagctc ctggaagagc
    33541 tgcaactgtt ttaaggccta ctgaagcatt aggtgcccca caaacgattt gaaccgtctt
    33601 cccatcgttg atttctactt gacagatgtg taaatggtca ctatctggat gatcaatcat
    33661 ttctttaatt tgcgccacaa caaatgtagg ttcaatatta ttaacaagag agtcttcgaa
    33721 tccttctttc agtagttctg cattaaggat tttaataact tgatcagata aagttacttg
    33781 acctactccc tcaatagcaa ttaaacttga cacttcaaag atattccaag caactgtttc
    33841 tttactatct tgcagataga cgcgtgctac ttgtccgcga cgatcaacat ctagcttagc
    33901 tccttgacta tccttaacaa taaccattaa ggtatcacca acatgctctc tattataagt
    33961 aaaaatcatt tattctcctt taaattttgc tattttttgt cttatttatg gttaatttca
    34021 gcgagaaatt tagtaatttc agcctttgtt ttgcgattct tattaaccaa acgacccagt
    34081 tcctgaccat tttccacaac aacgaaactc ggaatcccaa aaatattcca ctgctgagct
    34141 aattctatat agtcatcacg attaacacgt acaaagacaa aatctgagaa atctttttca
    34201 attgatggca ttactggata tataaattga caatctggac accaatctgc tgtaaaaaag
    34261 aaaacaactt tcttagtgct atcaatataa gctgctattt cttcataaga ttctggtaaa
    34321 atcatttgct cacctccaca taatcaagaa caccttgacg ataaatgaat tcgaaaattc
    34381 tgccatcttc caagacaaga ccaccactgg tcattttaat atcagattca aattcattaa
    34441 aataaaatac ctgaatatca cccatctgac taaaaagttc tcttaaggtc tgagtgattt
    34501 gctgacgttt ataatctgtt acttttttag ttagggtata accagcagct gccagtaaac
    34561 ccacaccagc aaggccagtc ccaaataaga tttttttatt tttcataact gttattatac
    34621 catacccccc ggaattagac acaggaattg atggaagaaa tccacataac agttatgaaa
    34681 aaaaacaact tattccttgt ggtataatag gatgacattg tcttaagata ccattatggt
    34741 taatcattca aacagaaaga aaaaacatga aagaaaaatt attagtacct gctttaaatt
    34801 gtttgctagg tctactcttc ctaggtgatc actggttttt atttcgtctt caaaatggag
    34861 aactttttgc tattgtgacg ctaatactgc tttgcacagc ttcattttta atcaataaga
    34921 atcaccccaa aaatttgact ttaattgcta tggttatcac ttttttagcc gtattatctt
    34981 tcctatctac ctttttatta gcttaagccg ataaacatta tctccgttgt attcaaccca
    35041 gttttcacta taacaggctt tgtcagtcat aacagtcttc tggtagtact tgatattctt
    35101 tttgaaagtc tttatattga ctgaaacctg ttctgttctt tatttttaat taaaaggtgc
    35161 taaaatagta tttgtagaat tgaggtaatt atgtcagatt tatttaacaa aattaaaacc
    35221 gtaactgagc ttgatgggat tgctggctat gaacacaata tccgcaactt ccttcgtcaa
    35281 gaaataactc ctttagttga tcaagttgag acagacggac ttggtggaat ttttggagta
    35341 aaaaatactc atgagactaa tgctcctaaa gtcatggttg ctgcccatat ggatgaagtc
    35401 ggctttatgg ttagtcatat tcagccagat ggaacatttc gtgtacttga ggttggagga
    35461 tggaatcccc tagtagtcag ctcacaacgc tttaccctct acacacgttc tggtgatgcc
    35521 attcctgtta tatcaggctc agttcctcct cactttcttc gtggacaaag cggtggaaca
    35581 acattaccca aaattagtga cattgttttt gatggaggat tcacagataa aaatgaagct
    35641 gaaagctttg gcattgctcc tggcgatatc attgttccta aatctgaaac cattttaact
    35701 gcaaatcaaa aacatattat gtcaaaagct tgggataatc gctatggtgt gcttatggtg
    35761 accgaattgc taaaaagctt aaaagatcaa agtcttagca acacacttat tgctggggct
    35821 aatgttcaag aagaagtcgg acttcgtggc gcacatgttt caacaactaa attcaaccca
    35881 gatatcttct tagctgtcga ttgttcccca gctggagata tttatgggga acaaggcaaa
    35941 ataggagagg gaaccttaat ccgtttttat gatcccggac atatcatgct taaagatatg
    36001 agagatttct tgcttacaac agctgaagaa gcaggtataa aataccaata ttatgctgca
    36061 aaaggtggta ccgatgctgg ggctgctcac ctaaaaaata gtggtattcc ttctacaact
    36121 atcggtgtct gtgcacgcta cattcattct catcaaacac tctacgctat ggatgatttt
    36181 ctacaagcac aagcttacct tcaggccatc gttaacaaat tagaccgctc gacggtggat
    36241 attattaaag gttattaaga tagaaaggat gggagtttca taaggtagac acattttagt
    36301 atctcctttc atccccatat caacattatg aaaattggaa ttatcggcgt tggaaaaatg
    36361 gccagtgcaa ttattcaagg cctcaaacaa acccaacatg acattgtcat atccggatct
    36421 tgcttagagc gttcaaaaga aattgcagaa agacttgatg ttacttacgc cgaatctcat
    36481 cagtcattaa tcaatcaagc tgatattatc atgttaggta ttaaacctca actatttgaa
    36541 aaggttcttc ttccacttga tatcacaaaa ccaatcatct caatggcagc aggaatctcc
    36601 ttagcgcgtt taagccaatt aactcgttct gatttaccat tgattcgtat tatgccaaat
    36661 attaacgcac aaatacttca aagttgcaca gctatttgtt acaacaatca tgtatcagat
    36721 gagttacgac aattagccaa agaaattacg gatagttttg gaagtagctt tgatatagca
    36781 gaaatcaatt ttgatacctt cactgctcta gcaggttcta gtcctgccta tatctatcta
    36841 tttatagaag ccttagctaa agcaggtgta aaatatggat ttccaaaaga acaagcactc
    36901 tctattgtag gacagactgt cttagcttct agtcaaaacc tattacaagg acaaaatagc
    36961 acctctgact taatcgacaa catttgtagc ccaggcggaa ctactattgc tggcctttta
    37021 gacttagaaa aaaatggtct gacgcatagt gttatttctg ccattgatgc tactattgag
    37081 aaagcaaaaa aactttgagc aaattgctca aagttttttt agttattcat cattgtactg
    37141 attaacacgc tataagcaag agcattgtta ataaaatgaa tcattatact atattctaag
    37201 tgctcagttt tataataaac aaaagataaa atagcaccca tcccagcata aataataaaa
    37261 ctacctatat cagaagggga atgaataaga gcaaaagcta aagtaccgat gacaaaaccc
    37321 catttttgat gtttcgcaaa aagttcttta gggataatat agcgaaatat aatttcttcc
    37381 ataattggtg cccctattat ggcaaaaatt aacatcaaag ctagtggaag ttgctttgtt
    37441 aattccataa ttactgtttg atttgctgta tctttaaccc cttgcatagt cataatgagg
    37501 taaccaataa tttgtggaac cctcaatgat aaccaaccta agagaatagt aactaatgct
    37561 ttccttgtca ataatacttt ccatctgatt atttgtttaa ccttggcata ataaaggaaa
    37621 agagcaataa taagtaattc taagccaatt aacaatatac tagtaaaagg cgtttgatag
    37681 tgattcttta ggagccacac agccaatata ggaacaagtt gattaataac taaaaatgct
    37741 attagcaaag ctaaaatttt gcctttttta ataataaatg acacaaaaac ctccaaaata
    37801 aattattaac ttttttctat cataacatat tttcaaatct tatagattta tctcacaata
    37861 acttctgtaa aataaagtaa attaataaat aaccgtataa aacaaaagag agtatcaata
    37921 gcaagaactt tgcataggaa aaagttatac caaaccagac attaatcaga cacaatacca
    37981 ctaaaacaag gacagataaa aggaaaagga gtcgacaaga catattagca ataaattgat
    38041 tacgctcatc aaaaactttg gtatagtaat gttgaaatcg cttttcacta aatgttatca
    38101 ccaaaaaata aaaacttcct gcaaataagg caatacctga accaaaggaa aaacctttag
    38161 taaaattaga ttcaaaggta tgtagaaatg atgatactat cataatcaaa ctacatacac
    38221 taaaaacaaa agacaaaaat ctcatatata ctttgtaatc cttcggagat actttactat
    38281 tgggtgacaa taatcctttt attaacatag aatactactt tctaaaactt caatttaatc
    38341 atcagctatt atatcgtttt cttcaatgaa gcatagcgat aaatagcaaa aatagacatc
    38401 gcaatgctaa tactaatagt actgataaaa acatattgct ggtaaacgat tggtggacaa
    38461 aaatcaatag tccacatcct tgacatatta cagattaact tttaaccttt tgtcttttgt
    38521 ggactgtatt tttccattaa aattctatcc tccttcactt tctgtataaa taaaaacttc
    38581 ctcaatttgt ttatcaaaat aacgcgctat cttaaatgct agctccaaag atgccgtgta
    38641 tttctctttt tctagagaaa taatagtttg cctagtaact ccaagtgcaa ctgcaagttc
    38701 tgcttgactt aatttcctag attttcgtaa cttttgtaga gaatttttca tatcttacac
    38761 ctttttgtat agttagcttt acatttatag tatagttaac tatacatttt ttgtcaagta
    38821 cattttacat acctgttcaa aaaacaaaaa agttgatagt taaactatca acttttctgg
    38881 tcttatcaat tttttgataa cttatttagc tttaaggcgc tcaacgtcac gcgcaatcat
    38941 taattcttca tcagtaggaa taactaaaac cttcactttt gaatctggtt ttgtgatatc
    39001 tccaaaataa ccaaaaacat ttttttctgg atcaagttca atgccaaacc atgataatcc
    39061 tgcgataaca tcttgacgca ttaatggcgc attttctccc atacctgcag taaaaataat
    39121 agcatccgcg ccatttaata ctgccaagta ttgtccaata aatttcttaa tacggtcaat
    39181 aaagacatta taggctaaca ctgcatttgg atctttactt tgaagacctg cttcaatatc
    39241 tcgcatatca cttgaagtgc ctgatactcc aagcaatcct gattgtttgt taagcatatt
    39301 aacaacagca gctgcatctt ccaattctgg atcattagct acaagataag ggataatcgc
    39361 aggatcaatg tctcctgaac gtgttcccat cattggtcct gcaagtggtg tgaatcccat
    39421 cgatgtatca atagactgtc catgataatt tgctgtaata gaaacaccat tacccacatg
    39481 tgctgtgatt aattttaatt cttctaaagg acgtcctaat tgcttagctg cttcttgtgc
    39541 aacgtattgg tggcttgtcc cgtgtgcacc gtatttacgt actttataat cagtatagta
    39601 cttttgtggg attggatata agtaggtatg aggttgcatt gttgtatgga aggctgtatc
    39661 aaatacacat acactcgtga tatctggtaa gatttctcta aacgcacgaa tccctgctgc
    39721 agctgctggg ttgtggagtg gagctaaagc tgataattct tcaacctgtt ctacaacttt
    39781 atcatctact aaagcagact ctttaaaata ttcaccacca gcaacgacac gatgaccaac
    39841 acctgtaatt tcattaaagt ctttgataat accatgtttt gttagatctt ctaaaagaat
    39901 tttaacagct tgggtatgat ctacgatatc taaaatttgt tcatcttttt tatcgtcaaa
    39961 ttttacagtc gaaatagaat ctttcaaacc aatacgttcg ataatccctt tggcaactac
    40021 tttctcctct ggcatttcat ataattgcca tttcaaactt gaacttccag cattaattgc
    40081 tattgttttt gacataattt acctctctaa agcgttttca caatctctat tctatcatat
    40141 tttttgagta atgactattt tctctgctcc atttttgaaa attttcaatg aagtttgcca
    40201 tattctcacg attttgcaaa tctgtcagcg gatatacaaa ggtttctggt ttttgttctg
    40261 cttgcttctt gagaacaaat atagatttcg cattttgacg acttccaaaa atagtttctg
    40321 gtagagttaa aacggcaatg acatctgcat atccttttaa ccactccttc agcaaatcac
    40381 tttgtggact tgttaaaagg ttttcgggtg ctaaaaatat agcgattcca tcttttttca
    40441 aatatttaag agattgctcc atcaatagat ggtgagcata ggtgtgctct ttagaacttg
    40501 atacagcata tcgtttagca atatcatcat taggataata gccaactggt aaatcactaa
    40561 tgattacatc gctttctttc aaaatttgtg gtctaacggc atcctcttgg ataaattggg
    40621 cactagaacc tataatttca gcaatgcttg ctgatagatc aatcaaaaga tcatcaactt
    40681 caatgcccat ataatttaac tctttcgagc tgttattgag gagagtctga gctaaattcc
    40741 cagttccact tccaatttcc aagacatcca ctgtctcttg actcgttaat tcttccaaaa
    40801 gaaataacaa gataaaacca atactatctg gcgtaaactg atgattagct tgtaattgct
    40861 ctgtttgcgc agatttgata aaaatgaact ggaaagtgcg acgccattct tcttgactta
    40921 agtcaagttg acgtaatttt tggttattca ccacaacaat atctaaatca caacttgaac
    40981 caaggtaata agagttctgt tcaattaagg catcataaat atgagttttt aattggttct
    41041 caatcgtttg gatattttct aaaatcagct cataggctgt ctcaattttt tcaaaattca
    41101 tgatttcctc cagaatttat cataacaaaa aagacagcat ctgaaaagag aagcaaacaa
    41161 aaatcctgtt tttaagcaaa accttgtctt actaatattt catcactgta tatttttaat
    41221 gtttatggct tgctttatta tcattagact tgagaggtac ttggagagtg tctattttac
    41281 gataaaactt atggttatag tttcctccct tatccaatgt aaccttaact tgtaaatagt
    41341 tcccttcttg gaggtatgaa gcatatcctc ctgtaaaata acattttcct gatatggaat
    41401 cgccttctat tgttttataa gctaactgcg ctgttagata agctttactt gcttcaattt
    41461 ggctatgata ctgtctttct gttgccaata ttcttgctag ataaaactgt aagacgagtg
    41521 taaatacagc agccaataca atagcttgca aaaggatgcc agctttaagt ttcttcttta
    41581 aaatcatagt aaaatgtcct ttttaacccg tccttaaaat aaaaaacaag ttttaccata
    41641 cttttggtct gactcatttg acaattgtct aacccataaa ccattggttg ataacctcga
    41701 ccattataac ctgtcttacg gaaatcatct ttattagatt tgccaaaggt tacaatctta
    41761 tcttgcttac gtaaataaag tttgttctgt cttaaatatt ccaagtgagc gccttcgaat
    41821 tctgcattta gttgctgagt taataacacc cattcagact gactggaaga agacatcact
    41881 actatctgtt gagccaacaa ttttgttagt ccttgataaa ctagcaaagc tcctgtgatt
    41941 gtaaccaatg ctaccaaaca ttctaaaatg tgtaaatgct ttaaccttct tatccttaca
    42001 ttttaacaat aaatttttca tgataatatc tccatatttt gatacattgg taacagcatt
    42061 gctgcataaa tcataacaat gataagagct acaaaaataa aaataacggg ttggattaat
    42121 tgggtcgctc tagctaattt tgtaaaaaaa tcctcccact tctcatcagc atatatatct
    42181 aactctgttc ctaatttcgc cttaacttga ccatattcaa tcattaaact aagctcagtt
    42241 aagaaaaacg gatagtctaa tactttttgg tgaaatgctt tacctgatag aaaaccttct
    42301 tccatgtcat atcctatttc cctaaaaagt ttggatttct gattttgcat tactttaaca
    42361 atttggtcca attcaacacc ttgacttaat aaatttcccc actcacgggc atagtaagca
    42421 gttaaataaa gcttaacata tgatccaact aagggaattg ttgttaaaaa acaagctact
    42481 ttaatgcgcg acaatcgctt ttgaataata taaaatatta aactaaaaat aagtacaact
    42541 gctaaaagta ataagaaaat attcggcaca tttgtaatca gtctagttgc aaaattattt
    42601 tctcctaatt ggggcattaa ataatgccta aggccaatca taattagcac cagaaaagac
    42661 aataatatca atgggtaagt agctacttct attacttttt ttctaactaa caaaagatta
    42721 gctaaataag actcaatctt tagtaggctc cttgaaatgt taccatgcaa atcagctaat
    42781 gcaacctgtg tgataacagt gtcagaaaaa cctaactctc ctaacatttt tgataaatct
    42841 tttccctcta ataatgcctt attcatccta tctgtataac aatcagacaa taacttactc
    42901 ctctttaaga aggtgaccat atcagttaaa gaaaaaccac tagcaaaaag attattaaaa
    42961 agttggacta ctttccgttg tttcttgagg gataattttt tcgacttgtg cctgtttctt
    43021 actgatatgt ccttcttcag ccaagatatc cacttgtcta ttccacttgt ctgatgagtg
    43081 ttttttaaag ttacttgtct caaagtcaat taggcttcct cctccaatta aacgttgata
    43141 tgctattaat tttagactat tttctaactc ttgatagtta acccctaatt ctataagcct
    43201 atcatagact ccgggaatac ttttagcatg aatagtagaa aaaaccatta ctcccgttaa
    43261 acttgcacga ataacagcac gggccgtcgc ttgatctcta atctctccga taattaaaat
    43321 atctggacga tgccgtaaag acagtttgat taaagcatca taagtcattc caatatcctc
    43381 attcaattgg agttgtaaca tcttgtcatt cttgatttct accggatctt caatcgtgat
    43441 aatttgctta tttttaaata cttctgaagc taattgatac atgagagttg ttttaccact
    43501 ccccacaggg ccggaaaaaa gatatagccc tcttgcacac agtacttcct tcatttgctt
    43561 tatattatca aaccaatatt ttaagtcctg atgacctgaa tacaaagtac gaataactaa
    43621 agattcttga ccacgataat ctcccacact tgatagtcgt aatgaaacca gtcttccctc
    43681 tgacagttca tagtcacaag aacctaattg acttcgtctt ttttctccaa cgttcatgcc
    43741 tgccacaaat ttaaagtgac taataagact agccatccta ttaaactcaa aaacatcaat
    43801 aaaccgcctt tcatcatcaa tacgcatata gagttcataa caatcacctt tgggaatgat
    43861 ataaatatct tgagcattta cttctactgc ctgatgaatg acgtgctttg ctaatgattg
    43921 aaccatcccg tacctcctta accatatatt cgaaaataaa ataaaaaaat ctcaccaatt
    43981 ggtgagattt tttcaatgat ttcttgaggg gtagtaacat aaatattatg aagcagaggc
    44041 tgaacaaacc tattatatcc ccataaaaac tatctaactt ttttaaacta cttttaatta
    44101 caaatttaaa gaacatgccg atggcaactc agctatctta ttaaatactt caaatcgctc
    44161 aatagattct tccttaggaa tttcttgcca ttctttcaat aacattaagg aatgatactg
    44221 ttggaggtac tcatcacatt cttcacacca acgtttttct tttatactcc aaaacgttgc
    44281 caacaaacta gacttacttt gaaaataagg ccacttttcc tgtcccctat cccactcctc
    44341 ttgaaaatag agcaaagctt ctgataaggt atcatactca gcaatttcag taatatcttc
    44401 ctcccaacct tcaataaacc accatggttc ccaatcacca aacattttta caacttgata
    44461 cataatacac ttcctttaga gagtattata taaaaaataa gaataaaata aaaaatatct
    44521 gctcattcca aattcttgta aaaaaagaac ctgagttaaa ctcaagttct ttttgattaa
    44581 tcttctgttg taggtgttga tacttccgta acttctgcat caacaggagt tccttcaata
    44641 atctcaactt cattaacagc aagtggttca atattacggt aacgagccat accagtaccc
    44701 gctggaatga ttttaccgat aataacattt tctttaagac caagtaagtg atctttctta
    44761 ccacgaatag cagcatctgt aagaacacgt gttgtttctt ggaaggatgc agctgataag
    44821 aatgaattag tttcaaggga agccttagta atacccataa ggacaggacg gcttgtcgca
    44881 gggattccac cagaaataac aatatctttg ttagcatctg taaagtctga aatatccata
    44941 agtgtacctg gaagaagatc tgtatctcct ggatccatga cacgaacttt acggagcatt
    45001 tgacgaacca ttacctcaac gtgtttatct ccgatttcta ccccttggct acggtaaact
    45061 ttttgtactt cagcaagtag gtacgtttca acagataaag tatcacgaac ttcaagaaga
    45121 cgttttggtt ggatagatcc ttcagttaag gccgcaccac gagctacttc atcaccaact
    45181 tcaactttca tacgtgctgt aaatggaaca acgtattctc cctcacctgt ctgaccttta
    45241 acgaatactt tcttagtacg tgttgaagag tcttcttcaa ttgcaacgac ttcaccctta
    45301 acttcagtaa tgactgcctc acctttaggg ttacgtgctt caaagatttc ttggatacgt
    45361 ggaagacctt gtgtaatatc agtatttgaa gcaacaccac cggtatggaa ggtacgcatt
    45421 gtaagctgtg tccctggttc accgatagat tgagctgcaa tagtaccaac tgcttcacca
    45481 acctcaacag catcacctgt tgccaagtta ataccgtaac agtgacgaca aacaccatgg
    45541 cgagtgttac atgtaaatac tgatcggata gttacttcct caacgcccgc tttaacaact
    45601 ttagcagcca tatcttcagt aattaaggta tccgcaccaa cgagaatttc acctgtctca
    45661 ggatgtttga ttgacttctt agtataacgt cctatcaaac gctcttcaag tgtctcagta
    45721 acctctttac catctgtaat tgctgtgatt gtcaaaccgc ggtctgttcc acagtcatct
    45781 tcacgaataa taacatcttg ggcaacgtca accaaacgac gagtaaggta acctgagtcg
    45841 gctgtcttaa gggccgtatc agtcatacct ttacgcgcac cgtgagttga gaagaacatt
    45901 tccaaaacac taagaccttc acggaagttt gataagattg gcaattccat gatacgtccg
    45961 ttaggagcag ccatcaaacc acgcataccg gcaagttgtg agaagtttga gatgttacca
    46021 cgagctcctg agtccatcat cataacgata gggttctttg gatcttgtgt ctcaataaga
    46081 cgtttttcaa gtgcttcttt agcttcacgc catgttgttg taacagcaac ataacgatct
    46141 tcttcagtca tcaaacctcg acggaaggct ttattgatat cttcaactct gtggtgagct
    46201 gcatcaataa tctcagcttt attgtcaata actgggatat cagcaatacc cacagttaaa
    46261 ccagcaagtg ttgaatgata atatccaagg tccttcaaac ggtcaaggaa agcagaagtt
    46321 tctgttgtac ggaaacgttt gaaagtttct gcgataatat taccaagatt tttcttctta
    46381 aagggaatat taatctctaa gttatcaata actgcttgaa tatcctgacc tggctctaag
    46441 aagtatttat ctggagtttt ttctgtcaag tttgcattat taggttcaat taagtacggt
    46501 aaatcttcag gcatgatatc gttgaagaga atcttaccaa ctgttgtaac catgatttta
    46561 tgtttttgct cttctgtcca aggcttattc ggcattgaat caacagcgat accaacacgt
    46621 gtatgtaaat gaacataacc attttgatat gccataactg cttcgtcatg atctttgaaa
    46681 atcatgcctt caccttcacg accagcatct tccatagtca ggtagtaatt tcctaaaacc
    46741 atgtcctgag atggtgttac gactggctta ccatctttag ggttaaggat gtgctctgca
    46801 gcaagcatca aaagacgagc ttctgcttgg gcttcttcag acaatggaac gtggatggcc
    46861 atttggtctc catcaaagtc agcattgtag gcctcacata caagtggatg aagacgaagc
    46921 gccttaccat caataaggac aggttcaaac gcttggatac ctaatctgtg aagagtaggt
    46981 gcacggttta gaagtactgg gtgctcttta ataacttctt ctaagatgtc ccaaatacgc
    47041 tcgtccccac gttcaaccat acgtttagct gctttaacgt tccctgcgag gtcacgagca
    47101 acgatttcac gcattacaaa aggtttgaag agctcaatag ccatttcacg tggtacacca
    47161 cattggtaca ttttaagtgt tggaccaaca gcaataacgg aacgtccaga gaagtcaaca
    47221 cgtttaccaa gcaagttttg gcggaaacga ccttgtttac ctttaagcat atggcttaat
    47281 gatttcaaag gacgactacc tggtccggta attggacgac cacgacgacc attgtcgata
    47341 agcgcatcaa cagcttcttg taacatacgt ttttcatttt gaacgatgat gccaggagca
    47401 tttaattcca acaaacgtgc caaacgattg ttacggttaa taacacggcg gtaaaggtca
    47461 ttcaagtctg atgccgcaaa acgaccacca tctaattgaa ccatcggacg aagatccggt
    47521 gggataaccg gtaaaatgtt aagaaccatc cattctggtt tatttccaga ttttttaaag
    47581 gcatctaaca catccagacg acgaactgct ttcacacgtt tttgacctgt cgctgatttc
    47641 aactcctctt taagaactgc gatttctgca tctaaatcaa cgcgtttaag aagatcttga
    47701 atggcttcag cacccatttt agcaacaaac gaaccgtaac cgtactcttg aagtttttca
    47761 cggtattcac gctcagtaag gagtgacttc ggttctaatg gtgtgtccat tggatcgata
    47821 acaacataag ccgcaaaata aataacttct tcaagtgcac gaggactcat atccaatgtg
    47881 agtcccatac gcgatgggat acctttgaaa taccagatat gagatactgg tgcttttaac
    47941 tcgatatgtc ccatacgttc gcgacgaact tttgcacgag ttacttcaac gccacaacgg
    48001 tcacaaatga ttcctttgta acgaatacgt ttgtattttc cacacgcaca ttcccaatct
    48061 tttgttggac caaaaataac ttcatcaaaa agtccttcac gttctggttt caatgtgcgg
    48121 tagttgattg tttcaggttt tttaacttca ccataagacc atgaacggac cttacttggt
    48181 gatgctaatg tgatttgcat acttttaaaa cgatttacgt caaccactag ttttaccttt
    48241 cttcgattta tcatcatact agcccagcta ggatttcaat aaatcctagg actagcattg
    48301 catatcaatt ctatattcgc ttctagttat aaactagata aatttaatct tcttgaacga
    48361 cttcagatac ttgttcagct tgtttttctt cagcttcttg tttcacacgc gctttttcaa
    48421 ggtcatcaac gtgcatcaca tcatcatctt caccttcatc aagatcacgg agttcaactt
    48481 cgttgtcatc ttcatcaagg acacgcatat caagaccaag tgattgcaat tcttttacaa
    48541 gaacacggaa tgattctgga acacctggtt ttggaattgg cttacctttt gtgatagctt
    48601 cataagcttt aagacgtcct gtaacatcat cagatttgta tgttaaaatt tcttgaagaa
    48661 catttgaagc tccataagct tcaagcgccc aaacctccat ctcaccaaaa cgttgtccac
    48721 caaattgagc tttacctcca agtggttgtt gagtaacaag tgagtatggt cctactgaac
    48781 gggcatgaag tttatcatca accatgtggt gaagtttgat catgtacatg acaccaaccg
    48841 acacacggtt atcgaatggt tcaccagtac gaccatcata aagaacagtc ttagcatctg
    48901 aatccatacc tgcttcttga acagtttccc aaaggtcttc tgaagaagcc ccatcaaaga
    48961 ctggtgttgc aatatgaata cctaaattac gagcagccat acctaaatga agctccataa
    49021 cttgtccgat attcatacgt gatggcaccc caagtgggtt caacatgata tcaactggcg
    49081 ttccatctgg taaataaggc atatcttcaa caggaacaat acgtgagacg acacctttgt
    49141 ttccgtgacg accggccatc ttatcaccga ctttgatttt acgtttttga gcgatataaa
    49201 cgcggacaag catattaaca cctgattgca attcatcacc atttgcacga gtaaagattt
    49261 taacgtcacg aactactcca tcgccaccgt gaggtacacg tagtgaagta tcacgaactt
    49321 cacgtgattt atcaccaaaa atggcatgca agagacgttc ttcagcagat aagtcctttt
    49381 cacccttagg cgttacctta ccaacaagaa tgtcaccttc ttttacctca gcaccaatgc
    49441 ggataattcc catttcatcg agatcacgta gtgaatcttc accaacattt ggaatttcac
    49501 gagtaatttc ttcaggccca agctttgtat cacgcgtttc agattcgaat tcttccaagt
    49561 gaacagatgt atagacgtct tctttcacaa ggcgctcact catgataacg gcatcctcga
    49621 agttataacc ttcccaagtc atataagcaa caactgggtt ttgtccaaga gccatttcac
    49681 cattttccat agatggtccg tcggcgataa aatcaccttt ttcaacgaga tcaccaactt
    49741 taactaaggt acgttggtta taagcagtac ctgagtttga acggcggaat ttttgaacat
    49801 gataaacatc aagagaacca tcttcgcgac gcacttcaac tttttcagca tctgaaaaaa
    49861 taacacgacc gtcatgttta gcaatcacag ctgcacctga atcgtgggct gcttgatact
    49921 ccataccagt accaacatat ggtgcttttg gatcaatcaa tggcacagcc tgacgttgca
    49981 tgttggcacc catcaaggca cggttagagt catcattttc caagaaagga atacatgctg
    50041 ttgcgacagc aactacttgc ttaggtgaca cgtcaacaaa gtcaacaatg cttgaaggaa
    50101 actcttggtt attaccttga tgacgaccca taacgatttc ttctgcaaat gtaccgtctt
    50161 cgttaagctt agagttggct tgtgcaactg taaattcatc ttcttcatca gcagtcaacc
    50221 aaacaatttc gttcgttacc gcaccagtag aacgatctac tttacgatat ggtgtttgaa
    50281 taaatccata tttattgagg tgtccaaatg aagataagtt attaatcaaa ccaatgtttg
    50341 gaccttcagg tgtttcaatt ggacacatgc gcccatagtg ggtatagtgc acgtcacgaa
    50401 cttcatatcc agcacggtca cgtgtcaaac caccaggtcc taaggcagag agacggcgtt
    50461 tgtgcgacaa ttctgataga gggttgtgtt ggtccatgaa ttgtgacagc tgtgatgaac
    50521 caaagaattc tttaacagcg gcagtcactg gacgaatgtt gatgatttgt tgaggtgtca
    50581 agacttcatt gtcttgtaca gacatacgtt cgcggacatt gcgttccata cgagcaagtc
    50641 cgatacggaa ttggttcgca agtaattcac caactgcacg aatacgacgg ttacctaaat
    50701 ggtcaatgtc atctacctta ccgataccct ctgcaaggtt taagaagtat gacatctctg
    50761 ctaaaatatc tgcaggagtt aaggcacgaa ctttatcttc aggatttgag tttccaacaa
    50821 tagtaacaac acggtctgga tcagttggag caacaacttt gaatttttga agaataactg
    50881 gttcagtaac tacagcataa tcatttggtg tgtagacaaa cttgtttaag tctccatcga
    50941 tatgctctgc aattgaatca atgacatctc gtgtcataac ggttccagct tcaacaagaa
    51001 tctctcctgt ttcaccatca actaaatttt cagcgattgt ttggttcaat aggcgtgttt
    51061 taagattcag ttttttatta atcttataac gaccaacagc cgctaagtca taacgacgtg
    51121 gatcaaagaa acgtgcaact aaaagactac gtgagctgtc tgcagttttt ggttcacctg
    51181 gacgaagacg ttcatagatt tctttaagtg cttcatctgt acgagaatca cttggatttt
    51241 tatgaatgtc tttttcaata gtattgcgaa caagttcact gtcaccaaag atgtcaacaa
    51301 tttcatcgtc tccagagaaa ccaagtgcac ggactagtgt tgtaaatgga attttacgtg
    51361 tgcggtcgat acgtgtataa gcaatatctt ttgcgtctgt ctcaagttcc aaccaagctc
    51421 cacggttagg gataacagtt gacccataac caacttttcc atttttatct accttatcat
    51481 taaagtaaac accaggagaa cggactaatt gtgaaacaat gatacgttca ccaccattga
    51541 tgatgaaagt acccatttca gtcataattg gaaaatcacc aaagaaaact tcttgagttt
    51601 taatttcacc agtttcttta ttaactaaac ggaaagtaac aaaaataggt gctgagtagc
    51661 tagcatcatg gatacgagct tcttccaaag tatatttagg ttcttttaat tcataaccaa
    51721 caaattcaag atccatagta tctgtaaagt ttgaaattgg aagtacatcc tcaaatactt
    51781 ctttcaagcc agcatcaaga aaatcttgga atgaatcagt ttgaatttca attaagtttg
    51841 gtaaatcaag aacttcctta attcttgaaa agctacgacg tgtacggtgt tttccgtact
    51901 gaacttcatg tcctgccaag tttttagctc ctttttaaag atagagtgac taagttttta
    51961 tctcataaaa cttagtgagt ttatttcatt tgctcgccta acaagaacaa gcttatagat
    52021 gtcgtttgtg gtaattttca gaaagtttaa atatatatta caataacctt ttttcttaaa
    52081 attaggcaca aaaagagcag ctaaatctca ctttatcaag ttgatttaac tgctgtatat
    52141 ctaggtcgga caatatttca acctagacag acgacaaata gttacgtaca ttataactta
    52201 ttttcaccaa taaatcaagt tttattctct tgacttcaag gaagcttcta gaatttcaat
    52261 ttgttcatca acacccttga gttttagcat aacacgatga tttttaaaca tagcatactg
    52321 gtgtttttct aattctttga aagaagacta tacaagaatt aaatcatatt atcttggaaa
    52381 aaataaaagg agaacagatc actctattct cctcaaataa aactatcagt atcgtaaacc
    52441 tgttatcgtt ttccacccaa agtagaccaa gctttttgat aatcactatc agttccccca
    52501 atagcaaaac gataggtcat cgttccaggt ccattttttg cccaataact tgttgtgctt
    52561 tcacctccaa cagtgattcg acgaccgttt actgttacta cacctggctg taaacctgta
    52621 gacttaagaa ctttagcttt tataacgcta tcatcaagtc taaacctttc tgacttacca
    52681 aaagtattgc catctgcatt attaatagca ttaaccaagt gagccatata attagcatta
    52741 ttattgtaac ctgtcaattt cgctaaagag gcattattat catgtcctgc ccatccacct
    52801 aaagtcactt taggtgtaga aagcatcaac caaacatcag aagttgagtt agtagtaccc
    52861 gtttttccaa tccaatctac cccagctaaa ccactattta aaccttgtaa gcgattttta
    52921 aaggttgtag tctttccgga gttaattggt ccatgtaata gctgctgtaa gatagtagct
    52981 gttgctttcg aaaagacacg gacaggttta ctttcgtgat tataaatcac tttcccatta
    53041 ctatcctcta tactttctat catatactgc ttatgataaa caccaccatt tgcaatcatt
    53101 tgataaaggt ttgtttgctg agcaactgac gtatcaatac caccaccaag tggtaaactt
    53161 tctataccaa agttttcaat tggatagtct aatttctcca tatagttttt tacatcaacg
    53221 cctcgatctc gtagcatctt gtaagtccag aaagctggaa tgttccaaga aatgtccaat
    53281 gactcttgaa ggttaaccat agcagtacct tcttcatccg catgcataat cttttcgcca
    53341 cttgaatatg tcgttggata atttgaaaga acactaccac ttcctaacat cccttgatca
    53401 atagcaattc cgtaaggtaa tatcggttta atacttgacc ctggagagcg tgccgtatca
    53461 aaagcatggt tattttgatt ctcactatag ttacgaccac caataaatcc tataatagca
    53521 ccactagaat tgtccgttag tacatttccc atttgaacct tgccagtacc atcatctaac
    53581 aaccctccgt attgagctgc agcatcctgc atggcttgat aaacagattt attgatagta
    53641 gttttaatag tataccctcc ctgttgaatc tcttcaatag cgcggtgtct gtacgtcgct
    53701 ctagtctcat cattttttaa atcatgctct gaaacattat cctttttaat gaggtaattg
    53761 tacatcactt tttgagcttc agataatgcc gagtagtaaa gataatcatg atgatttgtt
    53821 gtcgccactg caggtttgat aaaatctttt ttgatgtcat aatctttgta ggatttgtac
    53881 tcatctttgg ttaaagctct cgttctatac atattgtata acacattttt ttggcgcttt
    53941 ataccaaaac taagatcttt atctgatttc aactgggcat ctgcagtata aggagaatat
    54001 acaataggac tttgcggtaa acctgcaagg aaagctgcct gaggaattgt taaatctttt
    54061 gcggaaactc caaaaatccc ttgtgctgct tcttcaatac ctgcaatatt ctgtcctttg
    54121 ttattgcgac caaacggtga tacatttaaa taatctgata aaatcgaatc cttatccatg
    54181 taacgctcta atgctagagc ataaataatt tcttttgatt tgcgtttaaa tgacggatca
    54241 tctcccaaaa tttgctgctt taataattgt tgtgtcagcg ttgaaccacc gctactctct
    54301 ccaaacccca gaacagatcc cgctgcagca cgtagtacgg ctttgggaac tacaccttta
    54361 tggtcattga aattttcgtc ctctgttgca atgatagcct ttttaatatt atcagatata
    54421 gcatcctttg ccactggtgt acgttgtaaa tcagtggcaa tctctgaaat ttgtgattta
    54481 tcagaataag ttaacctaga caccctagta agtgtattaa cttgtgtcac taaactattt
    54541 ttacttggta ctttaacaga gtcaacttga ctagcaagat accctactgc aagtcctact
    54601 ccgagcataa caaatagtaa aataataaca tagatgaaat ctgataaaag ctttacaatt
    54661 cttaaaaaaa tagaaccaaa ttcaagtggt gtgtaatcac ctaatttaga actattcaac
    54721 ttcttattac ctttaaacat gaataatacc tcatcctaca ttatatcaga tttaatataa
    54781 cttgcatatc ctaaagagtt ttgatatagt tgacattaat acacaacaag ttaataatta
    54841 ggagaaaaag atgaatattt ttgatgaact caaagaacgt ggactcgttt ttcagacaac
    54901 tgacgaagat gctttacgca aagctcttga agaaggttct gtctcttact atactggcta
    54961 cgatcctacg gctgacagcc ttcatcttgg acatttagtt gctattttaa cctctcgtcg
    55021 cttacaactt gctggtcaca agccttatgc acttgtggga ggcgcaactg gacttattgg
    55081 ggacccttca tttaaagatg ttgagcgcag tttacaaaca aagaaaactg tagttagttg
    55141 gggtaataaa atccgcggtc aattatctaa ttttctagag tttgaaacag gtgataacaa
    55201 agctgtattg gtaaataatt atgattggtt cagcaatatt agtttcattg atttcttacg
    55261 agatgttggt aaatatttta ctgtcaatta tatgatgagt aaagaatctg ttaaaaaacg
    55321 tatcgaaaca gggatttcct atactgaatt tgcttatcaa attatgcaag gatatgactt
    55381 ttacgaatta aataagaact ataatgtcac tttgcaaatt ggtggttctg accaatgggg
    55441 caacatgact gctggaaccg aacttattag acgaaaatca aatggtgtat ctcatgttat
    55501 gactgttcca ttgataacag attctacagg aaaaaaattc ggaaaatctg aaggaaatgc
    55561 tgtttggctt gatgctgaca aaacctctcc ttatgaaatg taccaatttt ggctcaatgt
    55621 tatggatgct gatgccgttc gcttccttaa gatttttaca ttcctaagcc ttaaagaaat
    55681 agaagatata cgtattcaat tcgaggaagc acctcaccaa cgcttagcac aaaaaacact
    55741 agcacgtgaa gttgtaactc ttgttcatgg tgaaaaagct tataaggagg ccgttaatat
    55801 cacggaacag ctatttgctg gcaatataaa aggattgtct gttaaagagc tcaaacaagg
    55861 tttacgtgga gtaccaaatt atcacgttca gacggaagat aacctcaata tcatagacct
    55921 tctagtcact tcaggagttg tcaattctaa acgtcaagct cgagaagatg tctccaatgg
    55981 agctatttat attaatggag atcgtattca agatttagaa tatactatct cagaaaatga
    56041 taagcttgaa aatgaaatta cagtcattcg tcgtggaaag aaaaaatact ttgtattaaa
    56101 ctttaaatag acttgaagtg taatcgatac tctgataata atatttcaag acaataaaaa
    56161 gtgtagtcaa ttgactacac tttttattaa tgaacaacat tcctgaagag acaactttta
    56221 aattatagtt attatacatg actaccttat ttttattaag aaataacacc aaacaaacag
    56281 cttagaattt ttaccataga gtaaatagtt atcgaaaaaa tatacatatc taatacccac
    56341 aattttttca taaaaacaac aaatatcgtc tgtctaacac caacgaattt attctaacct
    56401 ttacatgtta taaatcaaga aaatatgatt agaatacctt aactttgtaa tcaaaatcag
    56461 ctaaagaaat aatccattaa aaaatctaat aaaataacaa atcatataaa aattattatg
    56521 taataacagt atatctataa catttaactt taataactat ctagttaaat aacataatct
    56581 ccaaaaaaat taactagtta tgttttttta ttactttttc ccaataaaat gataaaatta
    56641 tacttgtgaa aggccttcac aaatatcatt tagaagggtt taggtttatg aaattatcta
    56701 aacaattata caaatcactc ccactattaa ctgtcgtact atgcgttggg gcattacaac
    56761 agaatgttga agctaaggct aaacattata agacgacatc ccatgttgag acacagtatg
    56821 taagtacttc ttcaaaaaaa tcttaccgtt tactcataat aagcaaatca aggtaggttc
    56881 attagataac ttagaaagag caacatattc tcacatacaa ttaagggatg cagatgagcc
    56941 taagataaaa agagaaagac tgacgtataa tccaaccggt tggcacaact acaaatttac
    57001 tactgaaaag ggtaaaacaa cttggcttat agaccgaggt cacctcgttg gttatcaatt
    57061 ttcaggaatg aacaatgttc ctgagaacct agttactatg accaaatatc taaatacagg
    57121 atttagcgaa aataatcctg atggtatgtt atactacgaa aatagattag atagttggtt
    57181 ggcaaatcat aaaaatttct agcttgatta caaagtgact ccaatatacg aaggaaataa
    57241 tttagtacct agccgagttg aacttcagta tgttggaatt gataaacaag gaaaacttct
    57301 agaaatcaaa ttaggcggcg gcaaagaaca agttgatgaa tacggcgtca caactgttac
    57361 attagaaaat acgtctccac tcgcaaaaat tgattacaaa actggtatgc taatcaaaga
    57421 agatgggaaa caggcagaag aaggtgaaga tccaaatagc gatgctgatg aaaacgaagc
    57481 agctattgaa ggtgcatctg atatagaaga aaatgctaac actaatactt ctgaatcaga
    57541 tacaaataat gtagctccca aaaatagaat cgtctacgtt gcaaataagg gtcgttcaaa
    57601 tacttattgg tatagtttag aaaacataaa aaatgcaaat accgctaata tcgttcaaat
    57661 gactgaacaa gaagcgttaa atcaacacaa atatcacagc actactgaag cacaataatt
    57721 acaatttact gccggcaaaa ttgccggtag tattttttat cctaaaacgc aaaaatagat
    57781 tcacattgcc ccttctatca acttaagcca gaaaacttag tagatacctc atcgagataa
    57841 tttctcaact gatccctcat aaaatatatt cgaaaaaaac agggagataa ataaaaaatc
    57901 tatcctagaa taagatagat ttttttattt aacgtttgcg aagcaagcct actaagctaa
    57961 ctaataggaa aatgccaata aaaatcatcg ttattgttgc actagcaggt gtctcccaat
    58021 aataagaaag gaaaatacca gctaccattc ccacaaatcc aattaacatc ccaagaaaaa
    58081 taacagtttt aaaatttcga cctaaccgca tagcaatact agctggtaaa accataatag
    58141 ttgacaccaa tagagcacct gcggcaggaa ttgttaacgc aatagcaata cctgtaacaa
    58201 cattgaacaa aatagacatt gtacgcacag gcaatccatc tacaaaagct gtatcctcat
    58261 caaacattaa tatatacatt ggtctaataa ataggatggt taaaataaaa gtgattaatg
    58321 caataacaaa caaagcaatc acttgttcct tcccaatagt gataatagaa ccaaataaat
    58381 actgttcaag actgacatta ccaacattat gagctttact cataacgatt aaagatatag
    58441 ccaaccccat cgacatcagg atagcagttg atatttccat ataatgtttg tatacagtac
    58501 gcaaatactc taatacaact gccgccaaag taacaacgaa aatagttgac cacgtaggcg
    58561 agatacctaa aacaacgcct aatgcaacac cggctaagga aacgtggctc aaagtatcac
    58621 tcatcaaact ctgacgacgt aaaattagga agatgcctaa aattggagca aaaatactaa
    58681 tagccacaac tgctaagagc gctcgttgca taaaatcata ggaaagcata tcaagtagca
    58741 tctgattcaa cctccatttc gtttgtatgg acattgaaac aacgccatgg taaagattgg
    58801 ttcctaacaa gatgaatatt tcggtcagca taccccttta cttcatcagg atcgtgagta
    58861 atcatcaaaa cagacttacc atgcttatga gcattatgat gcattaactc ataaaatttc
    58921 tcagtagtcc ctgcgtccat accagttgtt ggctcatcca atacaaaaat atctggatca
    58981 gaagcaaaca tcctagcaat gacagccctc tgcttttgac cgccagataa acttcctatc
    59041 tttttatggc gattatccca cataccaaca gcttctaaac taaccctaat atgttcttca
    59101 tcatgtttag tcagtcttct aaaccaacca tttctagggt atcgtccaga tttcacaaat
    59161 tcataaactg acgatggaaa gccagcgtta aaacttgcaa tctgttgagg taaataggca
    59221 atacgtaatt tttttccttc tttgttttct ttagagatat taactgtacc cactttgggc
    59281 gttaaaatac ctaaagtagc ctttattaaa gttgatttag cagccccatt ttcacctgtt
    59341 aaggtaacaa actccccact gtctaaatgg taattaaccc cttccagcac aggatcgcta
    59401 tcatactgaa aagtaagacc actaactgta atatatcgca tgattaccct tctaattctc
    59461 tagagaaaag atcaagaaaa cgttctaaaa cgaccttttc gtcctttgaa aaatgattta
    59521 ctaatcttcc gtaaacccct aacgtattgt catgatgatg tgtgtgttca tctgcaatgg
    59581 gtttagcaag ttcagataac tcaaaataag taatacgagc atctttagaa tctttattcg
    59641 ctttcaacat atcctgagaa attaaacttt ttactgcttt agttacagct gcctgactaa
    59701 tatttaactt cttagctaaa tcagaatttg tcaactgctc ttgtgataaa agcatcagaa
    59761 tgtgttcttg cgtattagtc aattttacat cactttgaca agtaccaaac aataattcat
    59821 gttgattttc tgccttaagc aagatttgac tcactaaatg gtctaatttt tgttctaaaa
    59881 ctgtcatata tacctctttt tttgttaacc agtaaattat atcac
//
LOCUS       DATRME010000004        58356 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRME010000004 DATRME010000000
VERSION     DATRME010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58356
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..584)
                     /locus_tag="VBC23_000227"
     CDS             complement(<1..584)
                     /locus_tag="VBC23_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO6842206.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFD"
     gene            complement(559..1398)
                     /locus_tag="VBC23_000228"
     CDS             complement(559..1398)
                     /locus_tag="VBC23_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6842207.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(1391..2227)
                     /locus_tag="VBC23_000229"
     CDS             complement(1391..2227)
                     /locus_tag="VBC23_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6842208.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(2340..3998)
                     /gene="recN"
                     /locus_tag="VBC23_000230"
     CDS             complement(2340..3998)
                     /gene="recN"
                     /locus_tag="VBC23_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6842209.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(4010..4483)
                     /locus_tag="VBC23_000231"
     CDS             complement(4010..4483)
                     /locus_tag="VBC23_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6842210.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(4470..5297)
                     /locus_tag="VBC23_000232"
     CDS             complement(4470..5297)
                     /locus_tag="VBC23_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO6842211.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(5290..6162)
                     /locus_tag="VBC23_000233"
     CDS             complement(5290..6162)
                     /locus_tag="VBC23_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6842212.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(6162..6377)
                     /locus_tag="VBC23_000234"
     CDS             complement(6162..6377)
                     /locus_tag="VBC23_000234"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6842213.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(6355..7695)
                     /locus_tag="VBC23_000235"
     CDS             complement(6355..7695)
                     /locus_tag="VBC23_000235"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6842214.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(7821..8657)
                     /locus_tag="VBC23_000236"
     CDS             complement(7821..8657)
                     /locus_tag="VBC23_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6842215.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(8654..9508)
                     /locus_tag="VBC23_000237"
     CDS             complement(8654..9508)
                     /locus_tag="VBC23_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6842216.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(9647..11341)
                     /locus_tag="VBC23_000238"
     CDS             complement(9647..11341)
                     /locus_tag="VBC23_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6842217.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(11628..12362)
                     /locus_tag="VBC23_000239"
     CDS             complement(11628..12362)
                     /locus_tag="VBC23_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6842218.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(12355..13047)
                     /locus_tag="VBC23_000240"
     CDS             complement(12355..13047)
                     /locus_tag="VBC23_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6842219.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKPM
                     EKYLGKGVKIDG"
     gene            complement(13188..13418)
                     /locus_tag="VBC23_000241"
     CDS             complement(13188..13418)
                     /locus_tag="VBC23_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO6842220.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13716..15977
                     /locus_tag="VBC23_000242"
     CDS             13716..15977
                     /locus_tag="VBC23_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6842221.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            16163..16618
                     /locus_tag="VBC23_000243"
     CDS             16163..16618
                     /locus_tag="VBC23_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6842222.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            16682..16984
                     /locus_tag="VBC23_000244"
     CDS             16682..16984
                     /locus_tag="VBC23_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6842223.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(17054..19846)
                     /locus_tag="VBC23_000245"
     CDS             complement(17054..19846)
                     /locus_tag="VBC23_000245"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6842224.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(20131..20901)
                     /locus_tag="VBC23_000246"
     CDS             complement(20131..20901)
                     /locus_tag="VBC23_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6842225.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(20911..21699)
                     /locus_tag="VBC23_000247"
     CDS             complement(20911..21699)
                     /locus_tag="VBC23_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6842226.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(21701..21955)
                     /locus_tag="VBC23_000248"
     CDS             complement(21701..21955)
                     /locus_tag="VBC23_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6842227.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(21958..22563)
                     /locus_tag="VBC23_000249"
     CDS             complement(21958..22563)
                     /locus_tag="VBC23_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6842228.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(22575..23249)
                     /locus_tag="VBC23_000250"
     CDS             complement(22575..23249)
                     /locus_tag="VBC23_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6842229.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(23255..24535)
                     /gene="ftsZ"
                     /locus_tag="VBC23_000251"
     CDS             complement(23255..24535)
                     /gene="ftsZ"
                     /locus_tag="VBC23_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6842230.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(24557..25930)
                     /gene="ftsA"
                     /locus_tag="VBC23_000252"
     CDS             complement(24557..25930)
                     /gene="ftsA"
                     /locus_tag="VBC23_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6842231.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            complement(26203..27339)
                     /locus_tag="VBC23_000253"
     CDS             complement(26203..27339)
                     /locus_tag="VBC23_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO6842232.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            complement(27343..28419)
                     /locus_tag="VBC23_000254"
     CDS             complement(27343..28419)
                     /locus_tag="VBC23_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6842233.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            complement(28422..29777)
                     /locus_tag="VBC23_000255"
     CDS             complement(28422..29777)
                     /locus_tag="VBC23_000255"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6842234.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            complement(29907..30152)
                     /locus_tag="VBC23_000256"
     CDS             complement(29907..30152)
                     /locus_tag="VBC23_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6842235.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            complement(30197..32038)
                     /gene="typA"
                     /locus_tag="VBC23_000257"
     CDS             complement(30197..32038)
                     /gene="typA"
                     /locus_tag="VBC23_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6842236.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(32270..32650)
                     /locus_tag="VBC23_000258"
     CDS             complement(32270..32650)
                     /locus_tag="VBC23_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6842237.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            complement(32662..33630)
                     /locus_tag="VBC23_000259"
     CDS             complement(32662..33630)
                     /locus_tag="VBC23_000259"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6842238.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            complement(33627..33836)
                     /locus_tag="VBC23_000260"
     CDS             complement(33627..33836)
                     /locus_tag="VBC23_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO6842239.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            33991..34386
                     /locus_tag="VBC23_000261"
     CDS             33991..34386
                     /locus_tag="VBC23_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6842240.1"
                     /translation="MIMSTIYFISLCMSFILSYYDIKYQEYPIFLWILFTISTIILTP
                     ITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCL
                     VMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
     gene            complement(34387..35019)
                     /gene="nth"
                     /locus_tag="VBC23_000262"
     CDS             complement(34387..35019)
                     /gene="nth"
                     /locus_tag="VBC23_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6842241.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(35132..36361)
                     /locus_tag="VBC23_000263"
     CDS             complement(35132..36361)
                     /locus_tag="VBC23_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6842242.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            complement(36354..37469)
                     /locus_tag="VBC23_000264"
     CDS             complement(36354..37469)
                     /locus_tag="VBC23_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6842243.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            complement(37459..37974)
                     /locus_tag="VBC23_000265"
     CDS             complement(37459..37974)
                     /locus_tag="VBC23_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842244.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            38099..39091
                     /gene="bioB"
                     /locus_tag="VBC23_000266"
     CDS             38099..39091
                     /gene="bioB"
                     /locus_tag="VBC23_000266"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6842245.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            39092..39630
                     /locus_tag="VBC23_000267"
                     /pseudo
     CDS             39092..39630
                     /locus_tag="VBC23_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(39698..40264)
                     /locus_tag="VBC23_000268"
     CDS             complement(39698..40264)
                     /locus_tag="VBC23_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6842246.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(40392..42131)
                     /locus_tag="VBC23_000269"
     CDS             complement(40392..42131)
                     /locus_tag="VBC23_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842247.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            complement(42121..43866)
                     /locus_tag="VBC23_000270"
     CDS             complement(42121..43866)
                     /locus_tag="VBC23_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6842248.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            complement(44062..44577)
                     /locus_tag="VBC23_000271"
     CDS             complement(44062..44577)
                     /locus_tag="VBC23_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6842249.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            complement(44577..45683)
                     /gene="rlmN"
                     /locus_tag="VBC23_000272"
     CDS             complement(44577..45683)
                     /gene="rlmN"
                     /locus_tag="VBC23_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6842250.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            complement(45734..46312)
                     /locus_tag="VBC23_000273"
     CDS             complement(45734..46312)
                     /locus_tag="VBC23_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6842251.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            complement(46381..47715)
                     /locus_tag="VBC23_000274"
     CDS             complement(46381..47715)
                     /locus_tag="VBC23_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6842252.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            complement(47790..48863)
                     /locus_tag="VBC23_000275"
     CDS             complement(47790..48863)
                     /locus_tag="VBC23_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6842253.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            complement(48853..49338)
                     /gene="coaD"
                     /locus_tag="VBC23_000276"
     CDS             complement(48853..49338)
                     /gene="coaD"
                     /locus_tag="VBC23_000276"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6842254.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            complement(49335..49625)
                     /locus_tag="VBC23_000277"
     CDS             complement(49335..49625)
                     /locus_tag="VBC23_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842255.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            complement(49637..50341)
                     /gene="rsmD"
                     /locus_tag="VBC23_000278"
     CDS             complement(49637..50341)
                     /gene="rsmD"
                     /locus_tag="VBC23_000278"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6842256.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            50307..50756
                     /locus_tag="VBC23_000279"
     CDS             50307..50756
                     /locus_tag="VBC23_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO6842257.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            complement(50801..51793)
                     /locus_tag="VBC23_000280"
     CDS             complement(50801..51793)
                     /locus_tag="VBC23_000280"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6842258.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(51908..52972)
                     /locus_tag="VBC23_000281"
     CDS             complement(51908..52972)
                     /locus_tag="VBC23_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6842259.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            complement(53132..54034)
                     /locus_tag="VBC23_000282"
     CDS             complement(53132..54034)
                     /locus_tag="VBC23_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6842260.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            complement(54195..55154)
                     /locus_tag="VBC23_000283"
     CDS             complement(54195..55154)
                     /locus_tag="VBC23_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6842261.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            complement(55425..56843)
                     /locus_tag="VBC23_000284"
     CDS             complement(55425..56843)
                     /locus_tag="VBC23_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO6842262.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            57857..>58356
                     /locus_tag="VBC23_000285"
     CDS             57857..>58356
                     /locus_tag="VBC23_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842263.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLA"
BASE COUNT        18458 a        11651 c         9071 g        19176 t
ORIGIN      
        1 atatcgaaac taaaattatc gtttactaaa tcaattgcag tcgctttcaa ataaatacct
       61 tctttatttt ttaaatcttg caaattaata ttaataaccg attttttagg ttttatatct
      121 acaaagtttg gcaatttata agatgatttt atatattgta acacatcttt ttctggaagc
      181 ggaagagttc ctactgaaaa tgaggtcact ttaagttgaa cagcgccatt tgttaaacga
      241 taaggttcaa aatagatgta taaaggcact tcataaccta ataattgata agagccttca
      301 aatagtatag aagatgaagc agcataaatc ttataattca tcttcttagt ttgatattgt
      361 tttaaataaa gtgcaattgt cttattcagt tgcgacttat tggtcgtaaa agtacctact
      421 tttactttat cttgtacccc agtcgaaatt tttcctgtat taggctcacg tacttgaatt
      481 aaacgacttg caatcactgc tgtgaaggaa aggttaattg ccaacaataa gagaaatgcc
      541 catttccaaa aatttaaatt acggccagtt ttttcttgtt tcatttattt tctccataac
      601 ggcgttagac atgatttgat agccaatatt attgggatga aaatggtctc cagtaaagag
      661 agcatcatta gtgatacttg cctgactatt tgatgactct gtaataccct ctttaccatt
      721 tattccctta taaaggcggt cattaattgg gacaaaataa acattttctg aagcatcaac
      781 tacttctttt gtagctttat tccaattatc aataacggtt tgcattttag ttaattgtgg
      841 aaagtttagg taaaaaggat tataaatgcc taaaacataa ataggcaatt taggattatc
      901 ttgtcttgct tttgcaagaa tttctttcaa acgttcctta tatgcttctg ctggtttctc
      961 aaaggaattt agtgataaat gactgagctc tttacgaata acagccaaga catcattacc
     1021 accaacagtt agcgtcaata aatcagcttt ctctaaatct ttttcgattt gaggatctgt
     1081 cgtcatacgt tttaaaattt gttgactagt attcccagac acaccataat taacagaagt
     1141 cacttggtaa gagtatcgat tatggagtga ttctgatagc agtgggacaa aaccaccttg
     1201 agaggttgta tcccccacac cttcggtcag agaatctcca agagcaacat agttaagtgg
     1261 gataactttc tttgttagga agtctttttt tgttaattta ggatttgatt ttggaatgat
     1321 caaggaaaaa atcccaaaag ataaaagaag acttaccaga aaaaaagaaa gtccggtaag
     1381 tatttttttc ttattcataa cgaaccataa cagcaaatgc atcctcacca gtatgagttt
     1441 ggatgattga gccagtttct aatacggata taggagcacc tagaacagct agtttttctt
     1501 taaagttatt agccatatca gctttaccac agtaggagat gccaatttca gcaatttttc
     1561 gtgtttgggc actttcaaca aaattatcta accatttgct gaaggtcttt agaccgcgac
     1621 ctttaacaat cggtactaat tcatggtttg ttagctccat aattactttt atgttaagca
     1681 atgaacttag gagacctgtt acacgtccaa tacgaccacc ttttactaaa ttttcaaggg
     1741 tcgatacgcc aataaataat tctgacttct ggcgtacttc ttccacacga gccaaaattg
     1801 tatctaaatc agctccctct ttagctaatt tcgcggcttc tacaacctgg aatttttgac
     1861 actggtctgt aaaagtagaa tcaataactg taacatctgc accagcaata ttagctccct
     1921 ggcgtgatgc ttcaatagtt cctgatagtg tatgcgttaa atgaattgca atgatatgtt
     1981 ccgcaccttc attcattaat ttttcataaa tttctgcaaa aacacctaca ggtggttgac
     2041 ttgtttttgg caattcctta cttccacgca ttaggttcaa gaattcccct tgtgctttta
     2101 agtcattgtc tgaatacaag gtaccatcta tcataactga caagggaaca actgtaatgt
     2161 caagctcttt aataagttct ggttcaatgg taatggaaga atccgttaca attttgattt
     2221 tactcatatc ctatcctatt ctatacttta atatttcaat acccttatta taacaaaatt
     2281 taagtgtatc aacattaatt tctcctaaca atgttagctt tttaaagacg ttaccaaagc
     2341 taactaccaa gtaattcttt agcttgagta cgtgcagtat cagttacatt gtttcctgcc
     2401 aacattttag cgatttcttc tacacgttct tcatagctta aaagccttac cgttgaaact
     2461 gttgaagagt cactggaaat tttttcaata aaatattggt aatccgctat tgcaataacc
     2521 tgagctaaat gagaaattgc taaaacttgt ccatgactac caatcttgtg aattttttga
     2581 gcaatagcct gggccacgcg accagaaact cctgtatcca cctcatcaaa tacaatggaa
     2641 gttttatctt ccttacgtga aaaagcagat ttaatagcta acatcaatcg agataactct
     2701 cctcctgaag ccacttttac caatggctta aagccctctc cgggattcgt tgaaatataa
     2761 aattcaacga tttcgttccc ttctttatta aattttcctt ttgtaaattg aacttggaaa
     2821 tcagctttct ccatataaag ctctgttaat tcttgtttga tttcattttc caattgttta
     2881 gctagcttat gacgctctaa acttaattga tttgctgatt caatcaaatc gtgttctaaa
     2941 atctttaatt ctttttctaa agcatctgag gactcttcac tacctgtcaa tagactatat
     3001 tctttagtaa tgttatcaaa atagtctaaa acatcattca catcaccgcc atattttcgc
     3061 gttattgtgt taataacatc tagtctattt tcaatttctt gtagtaaacc agcgtcaaaa
     3121 tctaaatcat cgataacgtc acctaaacgt ttagtaactt cttcaataac gtagtaagct
     3181 tctgaaagat tggtggaaag atctttatat tctcgatcaa attcttctaa agccataagg
     3241 tcattcattg cagaacgaac attcgataaa cttgaaaact cttcgttatc taacataaga
     3301 tatgcatttg tcaaagtatc tgcaatattc ttatgattca ttaatttatc acgttgcttg
     3361 agtagcgttt ggtcttcatc tgatttaagg gctacagact caatttctgc tatttgaaat
     3421 tctagcattt caatacgtga tttattctct tgttcatttt tttgcttatc caatacacgt
     3481 ttacgaagct gacgataagc atcaaaaaga ctttgataac gctcttttat gacattaaat
     3541 tctgtattac caaattcatc taacatcaga atgtgcatgt taggtttcat tagttcctct
     3601 tggtcatgct gaccatgaat gtctaccaaa taatgcccca cagcttttag tgttgacaaa
     3661 ttcaccattt gcccattaat tcgactaacg ctgcgcccat tttgaaagat ttctcggcga
     3721 ataattaatt catctgctaa ttcaatgcca ttttcttcca ataattggac taatgattga
     3781 tttttttcaa tagagaaaaa tccttcaatt tctgctttgt tagcaccatg gcgaatcact
     3841 tcaacactag cacgggctcc taacatcata ttcatagcat caataatgat agatttccct
     3901 gcaccagtct caccagttaa aacagtcatt cctgtttcaa aattaagtga aatttcttcg
     3961 ataatagcga aattcttaat agaaatttct aaaagcataa ggtcctctac taatcttttt
     4021 tctccaacat tgcaatcatt gattgacgaa tgtgatcagc atctgcttct gattttgcaa
     4081 tcaaaaggag actattatcg tcagctgtca aactaaaaat atgttcttga caatgtgaca
     4141 ttatgaaggt tttaattaat tgactgttac ctggtatgac attaatattg ataaattgct
     4201 ctaaacctag tagcttatct gatattgata aaatacttgt tttaataggc tttgccacag
     4261 cagttgtaaa gatagggtcg ttttcatttg acaaaccgta aatatagcga ccttttgctg
     4321 atggcacttt tataatgcca atttcattca tatcacgtga aatagttgct tgagttagag
     4381 ttaccccata agcttccaat ctgcgtaaca actcatgttg tgtttcaaca gcatggttaa
     4441 gaacaatttg cttaattaaa tttaaacgtt cactcttctt catttttctt aaattcctta
     4501 tgtgcttttt ctataacatc ttgtatttgg tcaagcacaa gattttgtgg atcttgacac
     4561 ttttgcaaat gcattaaaaa ctcaatattt ccatgtccac cttgaacggg cgaaaaatca
     4621 agatgtttaa ccgtatatcc ataatctttc gtgaaattgg tcactgttgt caaaaccttt
     4681 tcatgaacca acttgtcttt gacaatacca tttttaccaa tttgctcacg acctgcttca
     4741 aattgtggtt taattaatgc cactacttgt ccaccatcca ctaaaatttc ttttagagct
     4801 ggtaaaatca aattaagaga gataaatgag acatctatcg atgcaaattc aggcagtccc
     4861 tccttgaaat cttctttttg ggcataccta aaattatatt gttccataga acgaacacga
     4921 tgatcctgac gtaacttcca aactaattga tttgttccta catctactgc gtaaactaaa
     4981 cgcgctcctg attgtagcat aacatcagta aaaccacccg tagaggcgcc aatatctata
     5041 gttagcttat ctgcaactga aatttcaaaa acttgtaaag ctttttctaa tttcaatcca
     5101 cctctactaa catattttag tttttcacct tttagtttta attcagtatc gtctgcaacc
     5161 ttttcgcctg gtttatcata acgttctcca ttgataacgt taatcaccag tcctgccata
     5221 acaccacgtt tcgcttgctc tcgtgtatca aaaagtccct gtttataggc aagaacatct
     5281 accctctctt tagccattca accgtaatcc ctctattata tctattattt ttctagcatt
     5341 aaagttagct tttttctcta gattttgaaa aattgcctga gcttttttca aagtatcgtc
     5401 aagtatttcc tgtgacttat ccaaacccaa taaatttgga taagttgtct tttctgctac
     5461 aatgtcttta tttggtgtct tacccaattc ttcaaaacta gcagtcacat ctaaaatatc
     5521 atcacgtact tgaaaagcat gaccgattaa aaggccagct tgatgcagtt ttccttttac
     5581 ttcttccgac ttctcagcta aaatacctgc tgcaacaaag ggataagcta acaggcgtcc
     5641 cgttttatta atatgaatca agcttaaatc gtcaatagat aaaactttgt tttctccctt
     5701 catatctaac atttgtccac caaccatgcc aaaactaccc gcagacatag acaataactc
     5761 tattaaccta acagtaacat cagctttaaa accagctttt acaactaaat caaatgggtc
     5821 taaaaaaagg gaatctccag ctaaaaccgc tgtagcttca tcaaactttt tatggtttgt
     5881 taagcggcca cgacgaaaat catcattatc catcgccggt aaatcatcat gaattaaaga
     5941 gccagtgtga atcatctcca aagcagcagc cacatcataa tgaccatcta ttagttcaac
     6001 tccaaatccc tctaaaattt ctaaaaggat gcgtggacga atacgtttcc cacctccatc
     6061 aactgagtag aggatagcct taattaaatc aggagaaacg acactatgtg tctgtttgta
     6121 atgacgatga atagcttcat caattttttc aatagtaacc atcaagtatc catttctact
     6181 tccgttccgt cagcttgcat cacttttaca agcgtctctt cagcttcttt taaagttttt
     6241 tgtaactctt ttgaaataag cattcctttt tgaaattcag caatggcatc ttctaaggca
     6301 acatcacctg tttctaaacg tgagacaatt gtctctaatt cctgcaaatt ttcttcaaat
     6361 gttttcttgt ctgacatcct tcacctcaac ttctaaaaga ccatcttgca tcttaacttg
     6421 caaatgatct ccttcattta ttccattagt agtagatact aaagtatggt ttttttcgat
     6481 aatagcgtaa ccacgcgcta caattctaga actatccaat gatataagcg catcttgagc
     6541 cttttcaaaa cgtgctagct tgctgtcata ctgactagtc attgtacttg ataatagtcg
     6601 tctactttgc acaacacgct cttggtaagt atgaagccgc tgttttaaat ctatgccttg
     6661 cagtttttga tgaagcaaac cttgcttttg tcttactgtt tgtagtttat cacgcataga
     6721 atatgttaac tgctgattta ggttatctaa tttttgaagg aaaccatcat atagacgctc
     6781 tggttgccta aaaataactg attgctttga tttttgcaac ctctcctctt ttgtcctgat
     6841 aagacgaagg ctagactgat acattctatt ttcacgttca gtaatccaag acaagatatc
     6901 aattttagta accgttgtag caagctcagc tgctgcagtt ggagttgctg ctctgcgatc
     6961 ggctacaaaa tctgctaaag ttgtatcagt ttcatgtccg acactagaaa taacgggaag
     7021 acgagattca aaaatagctt ctacaacaca ttcttcatta aatgcccata agtcctcaat
     7081 agaacctccc ccacggccaa caattaatag atccaaatcc tttttctcgt tagctaaagc
     7141 aatagtttgt gctatctcct gagctgcgcc ctctccttga acttttgtgg ggaaaagtaa
     7201 tatttccact cctgggaatc gacgagatac tgttgtaata atatcccgaa taacagctcc
     7261 acttggacta gtcacaactc ctatttttct aacaaattga ggaagtaatt gcttatgacg
     7321 atcatcaaag taacctgctt gagagagttt cttctttagt tgttcaaatt ggatagcaag
     7381 agctcctata ccatccggtt ctgctttttc aacaataata gagtaggagc cgcttggttc
     7441 ataaagttga acacggccaa ctacattaac cttcatccct tcctctaatt caaaacccag
     7501 ttttttgaat tgacctgacc acattgtcgc ctgaatgact gatttatcat cttttaaaga
     7561 gaagtattga tggtttgggc gacgacgaaa attagacact tgcccagtta aataaaccct
     7621 ctctaaataa gggtctttgt caaattttaa ttttaaatac ttagtcagtg tcgacacgga
     7681 taaatagtct gacatagcac ctcctcatga gagttttatt aacttcatta taccaaaaaa
     7741 acttaaaaaa tacctatttg ctctacaaat agctcgataa agcaaaagaa gtgtcatgct
     7801 aacacctctc tacttatttt ttatttagat agtctagcca tttcttttgg aatctcatca
     7861 ctaatcgttg tcggtagaac aacgtaggct tctttagata attcttgagc aattgccgaa
     7921 tgtagataaa ctcccactga taccttatct agaggagagg cttctttaaa ttgcgctaac
     7981 atacctgcaa tcataccaca caaagtatcc cccatccctc cagtcgcctg gtaaggacct
     8041 cctactataa tttctttttc gtctaaatct tgaaaaatac gcgtatgcga actcttagcg
     8101 actaagatcg tccctttggg aaaagattta agagcggttt gggtattttc ttttgtctgt
     8161 tgcgatacag caatacctga caatcgctcc cattcttttt gatgaggtgt caacactaaa
     8221 ttcttagcct ttgtttgcgg aaacgcacct tctgataaca gagtcaaggc tgatccgtca
     8281 atgatgacac tttgatgtga ttgaatcttc tccatggtcc ggttcaaaat ttttcgagat
     8341 gattctgata ctcctaatcc aggaccaatt acaataacat cactttgaac aattgatttt
     8401 tccaaccatt tgtaatcatc actatcaaac gccattacct ctggtagttg actatgcaaa
     8461 gaggggatat tgcaactttg ggttgctaca gtaactaatc ctgcaccagt tttgacacag
     8521 gccaaagctg ccattataat agcacctcca tagggataaa aacctcctat cagaagaaca
     8581 ctaccataat cacctttatg gcttgattta gggcgtggct taattaacgc cctcgcttct
     8641 tgttcaccaa caatcataga ctcacacttc taagagctgc ttgataagtt tgttccaaaa
     8701 gcattgtaat cgtcataggt cctacgcccc caggaacagg tgttatcata ctagcaactt
     8761 ctgccacttg ttcaaataca acgtctccaa tcaatttacc attttcatcg cgattcatac
     8821 caacatcaat cacaacagca ccttctttaa cgaagtcttt tgtaacaaag tgcccctgac
     8881 caatcgcaac aataaggata tcagcctcct ttgttacctc agaaaggttt cgagttcttg
     8941 aatgtgtcaa agtaactgtt gcgtttttat caaggagaag ttgtgccatt ggcttaccaa
     9001 caatattgga ccttccgatg ataacagcat gttttccctc taaatccaca tggtactctc
     9061 taaacatctc cataatacca gctggcgtac aaggtaccat catcggtcgt cctgaccaaa
     9121 gatgacccgt attcattggg tggaaaccat caacatcttt cttaggatct attgctaaaa
     9181 taattttttt atcgttaata tgttgtggta acggaagttg aactaaaata ccatggatac
     9241 ttttatcttc attgtattga tggataatat caatcagttc ttcttgagaa atggattcag
     9301 ataatcgtaa agtctcactt ttaaaaccag cttctaatgc agagcgttct ttatttcgaa
     9361 cgtacacttg gctagctgga ttatccccaa caaggattac agctaaacca ggtatgatac
     9421 catgctgttc ctttaacctt tcaacttttc gccctaactc agcctgcatt ttttgtgata
     9481 aggctttacc atctattaat tcagtcattg atactccttc ttcatccgtt ttttctatat
     9541 tatatcaaaa aatagcgatt atgctatttt atatcgctat tcattttgta tcaattttaa
     9601 tcaatagaaa aggcaatgaa tccctcatcg cctttttaca tatgacttat ttagtactat
     9661 tcattttagc acgacaagcc gactcaattg cattaagttt cgataaacta tctgcttctg
     9721 tacaaccaat cgtataaagg taacatttta tcttaggttc tgtccctgac ggccttaaag
     9781 cataccatga accctcatta aaataatatt ttaaacaatt ttgctttgga aaatccttat
     9841 aaccatcctt gaaatcaata gaattctcca atgtcatctc acctacttgt aatattgggt
     9901 tctgtctaaa atcctccata atacgactaa tacgttcttg cccctcagca ccctctaact
     9961 caagagaaaa ttggcgctcg ttgtaatagc caaattcatc gtaaatggtt tgcaaaacgt
    10021 ctaaaagtgt ttgccctcgt tctttataat aggcagtcat ttctactacc atcattgaag
    10081 cactcacagc atctttatca cgtacaaaag tgccatagca aaaaccaata ctttcttcat
    10141 agccaaagag ataagtttta tcctttgaga tatcatattc attagctttt ccacaaatat
    10201 ttttaaatcc tgttaaagtt tcaacagttt caatattata tttatctgca ataacttttg
    10261 atagatcacc agttacaatg gattttacca atacaggatg atgtggcaaa ttccctaagg
    10321 cacatcgttg tgaaaaaata taataggaaa gaagtgcccc tatcttatta ccatttaaga
    10381 aaatatattc tcccttacta tctttgactt ccaatgctac tcggtcacaa tctggatctg
    10441 tggcaagtaa gacatctgca tcaactgact ttcctagaga ttctgaatag gcaaatgctt
    10501 taggaacttc agggtttgga tagccaactg ttgtaaaatc aggatcgggc atttcctgct
    10561 caggtactac ataaatattt tcaaaaccac ggcgtcttaa aacttcgcgc acaggtaaat
    10621 ttcctacgcc atttaaagga gtataaacta ctcggactga cttatcaatg ttagtatcat
    10681 taatagttaa accaagcact tcttttttat atgcttcttc aatactctca tcaatataac
    10741 ttgccaaacc acttgccaga gcctcttcaa agggtatttg cttaatttgc tgataatcgg
    10801 ttatagcatc catatgattg gcaatttggt cagcaatatc atctaaaatc tgagatcctt
    10861 ctttccaata tgccttataa ccattataag cttgaggatt atgactagca gtaaccatca
    10921 cacccgaaac acatcctaga gcacgaatag catatgagca cattggtgtt gggcgaatcc
    10981 ctttataaat ataagactta ataccatttg ctgccataat ggaacaagtt aattctgcaa
    11041 attccttaga ttgataacgg acatcataac taactgcaat tccacgtgca atagcttcag
    11101 ggccatgatc aataatcgta ttagctaatg cttgtgctgc tttccccacc atataagtat
    11161 tcatacgatt ggttcctgct ccaagtttac ctctcaatcc cgccgttcca aattctaatg
    11221 ttttgtaaaa acgatcctga atttcagatt catctccttt aatagcttct aaatctgact
    11281 taatatcttt accgagtgag tcgttttcta accactcttg ataaatctct ttataattca
    11341 tatgagacat cctattctaa gaccaaaatg gtaaaaatca gctccatgat aagagatttt
    11401 agttcttcag gagctaactg aatagtatca aattaacctt ataaagtcaa cttattttat
    11461 aaattaacta cacttaatat atttaaagca tctaaatttt tttcatcacc ctatcattct
    11521 taatctacat tatatataga cttaccaact tccaaacaat acagtaacaa tttactcata
    11581 acaatggaat aataaaagtc taaaacaagt tgttttagac aatgaaatta taatacttta
    11641 cttaagaagt ctcttgtgcg gatttctttt gtctgctcaa atactttctt aggggtccct
    11701 tgctcaacaa taatccctgc atccataaaa atgacacgat ccgctacttc acgtgcaaaa
    11761 cccatttcat gagtgacaat aaccatcgtc ataccagatt tagctaaatc ttgcataaca
    11821 gtcaagactt cacctaccat ttcaggatca agagctgaag taggttcatc aaaaagaagg
    11881 acatcaggat tcattgcaag acctcttgca atagcaatcc gttgttgttg tcctccagat
    11941 aagcttgctg gataagcatt agccttctct ttgagtccaa ctttttcaag tagctcgtat
    12001 gcttttgtct gagcatcaag cttagaaagt ccctttgtct taataggtga taaagtaata
    12061 ttttctagta cagtcatatt gggaaataga ttgaactgtt gaaaaaccat gcccattttt
    12121 tcgcgcattt taaaaatatc attctttttg tctgttatat caatcccttc aaaagtcact
    12181 gttccctttg ttggtacttc caagagattc attgttctta aaaatgttga cttaccagag
    12241 ccagaagggc caataataac caccacttct ccttgatgaa tatccaagtc aatgcctttt
    12301 aaaacctcat ttttcccaaa acttttatga agtccttgta ttgaaataat tggctcaacc
    12361 atctattttt acccctttcc caagatattt ctccattggt tttaacaaag ctgagagaat
    12421 cgttgtcaac attaaatagt aaaatgctgc aaataataac ggtgcaactg gtgagtaagt
    12481 agccgttaca actgattgtg ctccgttcca taattccatg acaccaatag tttgaaggag
    12541 agcactatct ttaataattg taataaattc attccctaga gcaggtaaaa tatttttaaa
    12601 agcttggggt aagataacat agcgaagtgt atttttaggt cgaatcccca acgagtaagc
    12661 tgcttctatt tgtccagatg gtacagcctc aatccctgca cgtacaattt ccgaaatata
    12721 ggcaccacta tttaaggaaa tgataatgat accaggtaaa agtcgtgtaa aatctaaatc
    12781 taaaacacca aagctaattg ttggtaaatt gttaaaatgc atccatgcga aagcaatcat
    12841 aatttgaact accatcggtg tcccacgaaa tacccataca tagaaattag ctaatattgt
    12901 gagaaaatgt aaattagtac gctttactaa agcaattaac acgcctataa tagttccaaa
    12961 aaaaacaaca catgttgaaa tcataatggt taccatcaca ccataattaa aataggacca
    13021 atattgtggt aaaaatgaaa aattcattga catctcctaa aaaagtaatt gccaatagtc
    13081 tatcacacaa agaataaaaa tacaagttta aataaatatt tttgttttta ttctatttta
    13141 atgtatattt agatataaaa aaagaccttc aaggtctttt ataattatca gtataataaa
    13201 tcgtaaattt ccattgcgat aaggtcaatg ctgtcaaatg aatatgattc acgcgcttct
    13261 acatcacgta atgtgaaaac aggaccgttt tcagggtcat tactaaaaga tacttctgct
    13321 acaacaacac cttcacgttc aaaattacgt tttaaattac caccatcagt cgccattaat
    13381 tcgaggcgat tgatgattct cactaaatgt gattccatct tacttactcc tatatatttt
    13441 ctagtctttt ctattttaac acaaaagtga ccaaagcaac atgaaaagca acatttttac
    13501 taacttttta acgattttat cgtctaaaat ccgcaaataa cgatttttct cagtagtcat
    13561 ctatttttca atttagcact ctaatatatt cagtgctaaa aacagaactt ttttcttgat
    13621 ttctatgctt taggtgctat aataaaagca taaagtttga ctatatttga ctatatacac
    13681 atcaatagtt aaatgtcata agaaaggggt agcctatgct ctgtcaaaac tgcaaactaa
    13741 acgaatcaac tattcatttg tatacaaatg ttaatggaaa acaaaagcaa gttgatcttt
    13801 gtcaaaattg ctaccaaatt attaaaactg accctaataa tccacttttt tcaggtttaa
    13861 atcatgtttc acatgcgcct ggtggcatca atcctttctt tgatgatttc tttggagatt
    13921 taaataattt cagagccttt aatggccaag atttaccaaa cactcctcct acacaagcag
    13981 gaggaaaccg aggtggagga aacggtaatg gacgaaataa taatcgcaac caaactgcaa
    14041 ctcctcctca agccaaaggg attctagaag aatttggtat caatgtcact gaaatagccc
    14101 gccatggtga tattgaccct gttattggac gtgactcaga aattattcgt gttatcgaaa
    14161 tccttaatcg tcgtacaaaa aataatcctg tacttattgg cgaacccggc gtagggaaaa
    14221 ctgccgttgt tgaaggactt gctcagaaaa ttgttgatgg taatgtgcct cataaacttc
    14281 aaggcaaaca agttattcgt ttggatgttg tgagtctagt acaaggaacg ggcattcgtg
    14341 gtcaatttga agaacgcatg caaaagttga tggaagaaat tcgtcaacgt caagatgtta
    14401 ttctattcat agatgaaatt cacgaaatag ttggagctgg tacagctggc gaaggtagta
    14461 tggatgcagg taatatttta aaacctgccc ttgcacgtgg agaattacaa ctagtaggtg
    14521 ccactactct caatgaatat cgtattatcg aaaaggacgc tgccttagaa cgccgtatgc
    14581 aacctgtaaa agtcgatgaa ccttctgttg aagaaaccat tactattttg aaaggtatcc
    14641 aaaaaaaata cgaagattat catcacgtaa aatataataa tgatgccata gaagcagctg
    14701 cagtactatc taatcgttat atccaagacc gctttttacc tgataaagca atagacttat
    14761 tagatgaagc tggttctaaa atgaacctaa cactaaattt tgttgatcca aaagaaattg
    14821 atcaacgtct cattgaagca gaaaatttaa aagcgcaagc gactcgtgaa gaagattacg
    14881 aacgtgcagc ttacttccgt gaccagattg caaaatataa agaaatgcag caacaaaagg
    14941 tcgacgatca agatacacct attattaccg aaaaaacaat tgagcacatc attgaagaaa
    15001 aaacgaatat ccctgttggt gatttaaaag aaaaagaaca atctcaatta attaatctcg
    15061 cagatgactt gaaacagcat gtgatcggcc aggatgacgc tgtcattaag attgcaaaag
    15121 ctattcgtcg taatcgagtt ggtcttggta gcccaaaccg tcctattggt tcctttttat
    15181 ttgtaggacc aaccggtgtt ggtaaaactg aactttctaa acaactagca attgagctct
    15241 ttggttcagc tgatagtatg attcgttttg atatgtcaga gtacatggaa aagcatgctg
    15301 ttgctaaatt agtcggagcg cctccaggat acgtgggata cgaggaagct ggacaactaa
    15361 ctgaaaaggt tcgtcgaaat ccttactcgc tcatccttct agatgaaatt gaaaaagctc
    15421 atcccgatgt catgcatatg ttcttgcagg tccttgatga cggtcgatta acagatggac
    15481 aaggaagaac tgttagtttt aaagatacca ttatcatcat gacctcaaat gctggttctg
    15541 gtaaaactga agcaagtgtt ggctttggtg cctcacgaga aggtaggacg aattccgtac
    15601 taggtcaact aggtaacttc ttcagccctg aatttatgaa ccgctttgac ggtattattg
    15661 aattcaaggc tttagataaa gagaatctcc tcaatatcgt tgatattatg ttatctgacg
    15721 ttaatgcacg tctcgccatc aatggtattc atctagatgt cactgataaa gtgaaagaaa
    15781 aattggttga tttaggttac gatcccaaaa tgggagcacg tccattacgt cgtaccattc
    15841 aagaacatat tgaagatgct atcacagatt actatcttga gaatccaagc gaaaaagaac
    15901 ttcgtgctat tatgactagc aatggaaata tcataataaa atcttctaaa aaaactgagg
    15961 aaagtacaaa aggttgagta agaaactcaa ccttttttca cactcttctc aaaaaataat
    16021 agcctctata aagctgtttc tctcctatct cgtctttaaa ttttaataca attattaagg
    16081 aaatattcca aaaattttag tataataaat aaaaaataat taaagctttt aaaatcataa
    16141 aacacaatca ggagagagaa ctatgactaa tcctactttc ggtgaaaaaa tagataatgt
    16201 agactatagg tcacgatttg gtgtctatgc tattattcca aatccaactc atgataaaat
    16261 tattttagta caggcaccta atggtgcttg gtttcttcca ggtggggaaa ttgaagaaaa
    16321 tgaaaatcac ctagaagctt taactcgtga gttaattgaa gaattaggtt attctgcaac
    16381 aattggccat tactatggtc aagctgatga atatttttac tctagacacc gtgatactta
    16441 ctactataat cccgcctata tttatgaagt tactgcctat cataaggagc aagcgccact
    16501 agaagatttc aaccatctag cctggtttcc tatccaagaa gctaaagaaa agctaaaacg
    16561 aggaagccat agatggggtg tccaagcttg ggaaaaaaat caccattcta ggaaataatt
    16621 catacctttc ttcctaaata gtgttataat aaacctaata agaatcaggg ggaaaatagt
    16681 tatgagacta attaatacaa ccagtagtca tcctgaacta gttcgaaatc aactccaaaa
    16741 tacagatgca aaattagtgg aagtctattc agctggcaac accgatgtcg tttttacaac
    16801 agcacctaaa cattatgaat tattaatttc aaacaaatat cgtgctatca aagatgaaga
    16861 attagaagct attcgtgagt tcttcttaaa gcgtaaaata gatcaatcta ttattattca
    16921 agagcagatg aaatcactcc atactgccaa gttaattgaa atttcatatc caacaacagc
    16981 ataattaaaa gaagcctaat caaggcttct tttttgatga ctactaaaca aatatagcat
    17041 taaataattt ttattaagct tcaaaacctt gagctactgc ttcaggataa tattgctcaa
    17101 taattgctgc actagcatca caaacagcta caccatacga acgcatcctt gtggttggat
    17161 caatacggcg tgagcgttcg cagacttcac cttcagcgtg ttcaacagta aatgcaacac
    17221 cttcaaatga tactgagtca gctggtttgt ctgcttcatc agcaattgtt aattgtgaga
    17281 caatcatcaa gagtgcaata tcgctattta acgctgttaa taacgttttc acttcttgac
    17341 tagcataaat tgttaaatgt gcttctagag atttaccaat gactttagcg ttacgtgctt
    17401 cttcgagagc tttttgagct tgtgttcgta acgtcatgaa tgctgaccac tcttcaagaa
    17461 tttcttcttg accagagaat gtctgtgcaa caggcatttc tgctaattga acaaattcct
    17521 cctcctcatg ttcaaggtat gaccaaattt cttcagcagt atgtggtaga atcggagtca
    17581 aaagttttgt aagtttaact aaaatatcgt agaaaacagt ttgcatacga cgtcgttctg
    17641 gactgttagc cgcttcaatg taaaccacat cttttgcaaa atcaaggtaa aatgctgata
    17701 gatcaactgt tacgaagtta acgacagctt tataaattgc cataaaatca taagcagcat
    17761 aagctttgtt gattgtatcc acaacttgat taaatttaat agtcatataa cgatcaacgg
    17821 cacctaaatt ttcataggct actgcatctt gtttaggatt aaaatcggaa gtatttgcaa
    17881 tcaagaaacg caatgtattt ctgatcttac gataagtttc tgatgtttgt tttaagatat
    17941 ccattgaaat acgaacatca ttacttgaat caacagaagt tacccacaga cgcaagattt
    18001 ccgcaccaaa ttgtttttca acatcactcg gaagaattgt atttcctaga gatttagaca
    18061 ttttctcacc tttaccatca agtacaaaac cttgagataa gactgctttg tatggagcgt
    18121 gaccattaac agcaactgat gtaattagtg atgagttgaa ccaaccacgg tattgatcag
    18181 aaccttcaag ataaagatct gctggatagg aaaggttttc acgagcattc attactccat
    18241 tccatgatga accagaatca aaccaaacat ccatgatgtc tgtttctttc tcaaagagac
    18301 cattaggtga acctggatga gtataacctg ctggtagaag gtccttagca tcacgttgcc
    18361 accaaacgat tgagccgtat tcagcaaata aatctgcaac gtgatcagtc acttcttttg
    18421 tcatgatagc tgtaccatct tctgcataaa aaattggaag tggaacaccc caagcacgtt
    18481 gacgtgagat tacccagtcg ccacggtcac ggatcatgtt ataaagacgt tttttacccc
    18541 actcaggttg gaaatttgtc ttttcaattt catcgaggat ttcttgacgg aatttcgaaa
    18601 cagaggcaaa ccattgtgga accgcacgcc aaatgattgg ctttttcgta cgccagtcaa
    18661 atggatatga gtggttgata acttcgctgg ctaaaagaag atctcccaat ttttctttta
    18721 caagtggtgt cactttgtca tagaattgac cttcaaaatc aggaccagca ttttccatca
    18781 tcaaaccacg actatctacg gtaactacaa catcaagtcc attagcaata ccaacgttat
    18841 agtcatcttc accaaaacca ggagccgtgt ggacaatacc tgtaccagaa tctgttgtaa
    18901 catggtctcc aaggataacc aactcttcta cttctgtatc ccatggatgt tctgtaacaa
    18961 tgtgattaag ttctttacca gtgtgatgag tcacaatttc aaagttttcc cagccaaact
    19021 tagcagcgag actatctaca agaacctctg caagaaggta tttacgctca ctacctactg
    19081 gtgcaactac aacatactcc atatctggtc ctactgttaa accgcgtgaa gctgttacag
    19141 taaatggtgt tgtcgtccaa acgacgatat aggtatctgt atcaagaatt cccttaccat
    19201 ctttaacttt attggcatag tagagtgatg tcgaatcaat atcatgatat tcgatttcag
    19261 cctcagcaag ggcagactct gatgaccatg accaatacac tggtttagca ccacgataga
    19321 tatatccttt atctgccata gcaccgaaaa cacgtacttg atctgcttca taatctggtg
    19381 tcagtgtaat ataaggattt tcccaatcag cagaaacgcc cagacgttta aaatcatcac
    19441 gttgtttgtc aacttggctg agagcataat cacgacacat ttcaaggtat tcagccaagt
    19501 ccatttcttt acgtttaacc ccttttttag ctaatacttg ctcaattggg agtccatgag
    19561 tatcccaacc aggcacataa ggagctcgaa aacctgacat tgattttgaa cgtacaataa
    19621 tgtctttaga aattttattt agtgcatgtc ctacatgaat attcccatta gcatagggag
    19681 gtccgtcatg aaggtgaaag gctggttttc cttcattcaa tgcttgacgt tttttataaa
    19741 tatcagcttg atcccatgct tcttgccatt gaggttcctt atttggaagc cctgcacgca
    19801 ttggaaaagc tgtttgtcct aaattcaagg tttcttttaa tttcatacta actccttatc
    19861 tcatagtaat agttatcaca attttttaaa ttgctaaata aaaaagcttt catcacataa
    19921 aggacgaaag ctcgtggtac caccttaatt cagagcagaa aatagctatt tcttactccc
    19981 tcaacaaccc gtaaggtggg ttaagccttc taccttactt aaataatagt tcaggtagaa
    20041 tgattgaaaa tgataatttc aatattaggg attgtagggc tctcaccatc tcctactcgc
    20101 tgataatata atattaaaac cttgtttttc ttaatcttca atatttaatt taaaagtttg
    20161 agtttcacct aaattgatcg gttcttccat ttggaaatta ttagtatcta ataaacctga
    20221 ctcttcaagt tgtttgttac tttcttcaac acgacgctgt aattcttcca tttcatctgg
    20281 tgagaattga cgagtagcat caaatgactc agtgtcatcc accactggta aagcatcatc
    20341 ttcatcaaga actttttcaa caacttcttt gaaagaagca tcagaatttt gaagataaat
    20401 agctgttggt ttcaacaatt cttcccaagc agatgagtta gctaatttta actgaccttc
    20461 taattcagaa agtaagcgtt ggtggaaaac acgtgattga cgtttcaaat cttctgtttc
    20521 tatagcgaca cgcttagcat catctgtcgc atcacgaagg atttgatttg cttttaattt
    20581 agcctcatca attaaatgtt gagcatcaaa tgtagctttc cccattaggt tagatgcttc
    20641 atcctgtgct gaaattttca cacgttcagc agtttcttga gctaaaataa ctgattgact
    20701 taacgattcc ttcatttcgt tgaagtaagc gattttctct tctaaatctt taatgtattg
    20761 ctcttgctca cgattacgtc taatcaagtc ttcgtaatcg tcaacaacaa tctctaaaaa
    20821 ttcattaact tcttcttcgc tataaccgcg aaattttgat gaaaatgttt tatctttaat
    20881 ttcaagtgct gtaagtggca tgttttctcc ttagttatgt atcattttgt ctacttctaa
    20941 cttatatttt tgatttttag ttaaccctag gttatggttc aacgtaaatc gaccatatcc
    21001 tctaacacta atcaaatccc cctctactaa ttgttcagaa actcgattga cgattgcata
    21061 attgaccttg actttatctg cctcaatcaa tttcgtcgac tgtgttcgtg aaatctttaa
    21121 aatcgttgct aaaattttat caagacgtaa actggaagca ataacagtta gtttttgaat
    21181 atcttgtttg ggagttagaa gtttggacaa gggaacttca gctaattgta cactagcagt
    21241 tccaattttt gtaactgaat gaacaaggtg atttgtcatt tgactatcga ccaaaacctg
    21301 agcacagcct tcttcaacta ggatatcccc taaaattgat cgttttacac ctaagtaatt
    21361 cagtaaagtt cccataattt tagcatgggt aaggtgatta aatttagcat tatactttat
    21421 ttcaattagg ctaaggttaa aatcaaccat atctaactga tagtattccg gtgctattat
    21481 tactttaaca tattctgttt gaaaatactg accagaagtg taataatgac tacctcgcct
    21541 ctctagaaca ctttcgagta ttttaaattc cctaggattt agaaattcag tggtaataaa
    21601 cgaataggtg ttttcgacat attgagctaa atggtctatt ttattgataa acgcatactc
    21661 ttctggtcga aaatgctgat aaatatcatc taacgtcatc ttatgtaaaa agcaagatta
    21721 aaaatctact caacatattt agtaaaaaga atgctactat tactgtaaaa tctagaccta
    21781 aaaattgtag attaaatctt ctgagtgggc gaataatcgg atcaacaatc tgtaccacta
    21841 gtctaccaat ccatgaatcg tatgctcccg gaagccaaga taacaaggca taggctagta
    21901 agatataaga atagacccta attgttttta ataatattaa tagtactaat atcatattta
    21961 tcgtctcttc atatcaaaat caaaactcgt ctcttgacca gtctttggaa tattcatctc
    22021 ctcaatatcg accataacat tggctggtgt taatagaaac attgaactcc caaccttttg
    22081 taatgaacca tataatactc tacttgcacc atcaatataa tccaagcaac gtcgtgcttg
    22141 cgcatcaagc atatattgaa aatcaatcaa gacacattca ttaacgataa gtaaatcaac
    22201 aatttcttgt gcatcctcat acttacgggg atacttcaga gcaattgtta cttgttctct
    22261 ctgttgtcga actgagtctt tattttgaat cctaggagta gcttgctgat aagcattgtc
    22321 tctctgtacc ctttgtctct cctgacgatt aggattatag gtagaagttc tcggacgact
    22381 accaatcatc tcttcctctg cggagttagt catatgacta cgctgagaag cttcctgtgc
    22441 tgtagctgct ctggaatctc tttgcactga agttctctct tgtacttcgt gtacttcatt
    22501 ttcacttaca tcgtcagtgt caaaataaga aataattttg tcaaatctat cttttaatgc
    22561 catattcccc tccattattt aaagaatgaa gttcctatcc ttacaaatgt tgatccattt
    22621 tgaatagcaa tatcataatc tctactcatg cccatgctta attctgtaaa cggcattcgt
    22681 tctatgttcc tagcttgaag ccttttccta agactctcag tctcttcaaa aattgatgat
    22741 atttcctctt tagttgcttt taagggagcc atagtcatga tgccgataag ttcaatttta
    22801 tcgtaccgag aaattaagtt cagtgcatca tctatttgct caatagtaaa accatgctta
    22861 ctgtcttctc gtgatatatt aacttgtaga aaacatttaa ctggtttttg agcacgtttt
    22921 tgaatctcag ctgctaactt aacagaatct aaagcatgaa aataatcaac ataattaatg
    22981 acatctttaa cttttcgacg ttgtaaacta ccaatgagat gccatgtaag cttttcatct
    23041 tttaatgctt gatatttttc aagaaattta tcaacacgat tttcaccgat atgattaaca
    23101 cctgttctaa taagcgcttc tgttgtttga cagttaacat attttgttac agctacgata
    23161 tgaactgatt caggagcgcg acctgctttt agtgctaatt tactaacatt gtcaaaaatc
    23221 gctgttttat tttcttgtaa attcatcatt ttaattaacg gtttttaaag aatggaggtg
    23281 tttcaagttc atcatccata tcatcatttt ctgaaaacgt tgacatagac aacttgctat
    23341 caagttcacc ttctgtcgga cgtgaaatat tatcacgacg aaggtcccaa ttaccaaaag
    23401 cagaactttg ttgctgattt tgtgcatgag gctgattttg ttgagttggc atttcgcgag
    23461 actctgtcat atcaaagttg ccacgacgat caaagtttga atttgaagta ctttgacgtt
    23521 ctgaaggtgc ttgattagtt ggagctgatg ttgtaaaacc agatacttga ttagttttat
    23581 ctttacgcac acctgttgca acaactgtta cgcggatttc gtctttcata tccatgtcaa
    23641 ttgaagtgcc taaccaaata ttgacacctt tacctgctgc ttggctaaca atctctgatg
    23701 cttcctctgc ttcagtaagt gtcatatcca taccgccggt aacattgaca ataacgtctt
    23761 ctgctccatc aatcgttgtt tcaagaagtg gtgaataaat tgcttttcga gcagcttcag
    23821 taatacgttc ctcaccagaa ccgataccaa ttcccatcag tgcattacct ttatttgcca
    23881 taactgtttt aacatctgca aagtcaaggt taattaaacc tgggttagta atcaaatctg
    23941 taataccttg aacaccttga cgtaaaacat tatctgcttc actaagagct tcaagcaatg
    24001 gtgtcttctt atctacaatt tcaagaagat tattatttga aatgataagc agtgtatcaa
    24061 cttgttctct taattcttga ataccttcga tagcgaagtt agaacgttta ttaccctcaa
    24121 agccaaaagg acgtgtgata accgctactg taagtgctcc taaactcttt gcaatacgtg
    24181 caataactgg agccgcacct gtaccagatc cgccccccat acctgctgtg ataaatacca
    24241 tatcagcgcc agtaagtgct tctgttaaaa cttcttcact ttcttcagct gctttacgac
    24301 caacttcagg ttgtcctcct gcaccaagtc cgcgagttaa ttttggacca agttgaatga
    24361 cagtttcagc ttttgagcta cttaaggctt gaatatcagt atttgctgca ataaactcaa
    24421 cgccagcgac accttcgtca atcatgcggt taatagcgtt accgccaccg cctccaacac
    24481 caataacttt aataactgca ccttgtactg atgctgtatc aaatgaaaat accattttta
    24541 ttcctcactt attgttttag tcaaacatac ttccaaaaat tcctctgatg cgctcaccaa
    24601 tattagtttt aggctcagta ggctcttgag cgttttctct tgctcggatt ggttctaaat
    24661 tagaatcttc catagaagag gttaacggct cagaataagg cattgtcgac attgtatttg
    24721 ttttttcttt ataatcaaag tcgactggtt tatgtcttag catttcgtcg ccagttacag
    24781 catgttgtgc gataatgtca acttctgaca tcattcctac ataatcaacg atactaataa
    24841 cattagcgaa cattggattg cgaataccta cttggtttgg aacgtgcaat ttaactctag
    24901 ttccaaaaat ttgttgagca acctcgacaa ctcctggcat aattgcacca cctccaacaa
    24961 gaataatacc accaggtaaa tcaagtaaac gacctctacc caagtcttgt ttgacatgtt
    25021 ctaaaatttg acgaatacgt cctgaaataa tttgcgaaag atagctttct gtaatttcaa
    25081 caggttcctc gttaccaaca acattaactt gaactgtatc agaagtactt gcttcttcaa
    25141 cattagcttg tccaaagttg aatttcaatg cttcggcaat ttctactgtt gtacgtaaca
    25201 ctttagaaat atctttggtg acataatcgc taccttcaga ataaatatta gtatattgca
    25261 attcttgatt acgcatagaa gcgactgttg tttgtccgcc tcccatatca atcacagttg
    25321 caccaaactc acgttcacct tcgtttagaa ctgattttgc taaggctaga ggggcaatca
    25381 caacatgctc tacttttata cctgctcgtt caacagtttt acgaagatta tgaaggatag
    25441 ttgttggtcc agtatagata agaccacgca tctcaaggcg aatccccatc atccctctag
    25501 ggtctctaat cccttggaaa ccatctacaa tgaattcaag cggaatcaat gaaataactt
    25561 ctctttcagg agtaatgctt ttagtcaatg ctgatttaac aacactttcg acatcttcat
    25621 ccttaatttc ttttgattca ttgggaacag gaatcattcc ttgagttggt tcaatttgga
    25681 gaagatttgc tggtaatcca acattgattt tgtcaatggt gatgcctgct ttctcctcag
    25741 cttgttttac cgcctctttg attgcagttg ctgctgcctc aatatcaata attataccat
    25801 cttttacacc ggaactaggg acgttgctaa ccccaattac attcatttca tttgcaataa
    25861 attctgcaac taaaacttta atcgagcttg ttcctatatc caaaccagta aaaaagccat
    25921 ttctagccat tcactcgacc tcgctatctt tccattcttt tctattctac atctgccaaa
    25981 aacagaatta ttcagaaaat attataccat aaaaaaagca gaattgttac caaattacgt
    26041 ttttttatta agcaacatca aattgaaata tttctaactt atttaaacca ttcgcaactt
    26101 ataacaaact taaatttttt tcaagaaatc aatttttact agcaatctat gactgacttg
    26161 atatgtataa aaaagactga taaatccagt ctttttttat cgctaattaa cattttgagg
    26221 gttgggtgcc tgctctgttg ctatctgttg tccttgttga ttagtatttg agttatttgt
    26281 ttgtccttga ctattttccg caacctgacc attttgtgtt tgtgttttat cagttgattt
    26341 attttttgta tcttccgctt tcacaggggt tgattcaata gtacttgttg ttgtgtaaac
    26401 tcccacttcc atatcaacaa tagaaggttc cttaaggttc ttcttaattt gtttgtaaaa
    26461 aggaagtctt tctttaaatt tagataatgg tattctaata ctatttccat catgcatatc
    26521 taacagcagg aggtcaggtg tcgttttaga atcagctaaa cttatcacct gaatctcact
    26581 tattaaatca gggtctaaag cctttaaatc tttaattaat agcttaatac tatcttcctt
    26641 atcaaggtta attgttaaga agtgctttgg tagctctgaa ctatttacag gatcagcctt
    26701 ttttccagtt tccaagacag gctgatatcc ttgctttgta tgtgcatatg caataatctt
    26761 attttcttga acttgaatat gaaacttatt gggaaattga taagtcatct gagctgtttt
    26821 tacccataca tcttctgcag ctaaacgttg ttcaatagct ttatgtttaa aaattaaaga
    26881 aaagaaataa tcgttttttt gaatattcgt tttttctatc aaaatatcat caggtgtatg
    26941 ctgattgcca ctaactgtta ttgttttttg cttactaaaa ggagttagta ggaaaacgga
    27001 aactaaaatg actaggaatg ctactacaaa tataggtgca gttctaatgc gattagtttt
    27061 ggctaagctg ttgactattt tttctttttt ctgtttcttt tcaatcttag gttttgaaat
    27121 ctttggaaaa tgaagcttct taattttagt agtattttga ggttcttcag gagaagaaat
    27181 atttaattta cttcttttat ctaagcgtaa tttttcgtta atacgttttt gttcttcttc
    27241 atcttctttg cgttttttta aaaattcaag gttacgcttt tgccattccg ttaagacaac
    27301 ttcttctttt tctggggtat ctgatttctt cttaggcatg ttttatcctt ttgttacttt
    27361 tgccatatca tcaattaata attgataaaa ttcatcttga gattttattt cagatgattg
    27421 tgacatgttt ttttcgtaac tctcactgtt agaaattaat aaatttatct gtttctcaag
    27481 tgtatttatg tttaattcag attctggtaa ctgcaatgcg tagccctttt cttcaaaata
    27541 agcagcattc tctaattggt cacctcggct cgcttcacgt ccaagaggga taataagatg
    27601 aagttttttc atagctacta attcaaaaat agtattagag ccacctcgtg taacaaccac
    27661 atctgctaaa ttcattaaag gctgataaag gtctgtaaca taatccacgc gataaaggtt
    27721 cttcttcaat cgattgagag aagaatcgcc tgaaatattg ataacattat atttttcttc
    27781 cagctcaggt gtttgagtaa taaaatcatt gaatactttt gcacctgctg atcctccaat
    27841 aaatagtaag gtctttaggt ttggatcgaa ggcttctttt atactagtta agtcagtatt
    27901 ttcaaacgac tttttacaat ccatgacttt tgttacagca ccaatatgct tagttttgat
    27961 taaatcttta gattgttcaa aagtcgtata cataatagtc gcaaatttat aagcaatctt
    28021 atttgctaac cccattgata aatctgattc atgaacaaaa acaggaactt ttaataggcg
    28081 tgcagctact acaggaggta ctgaaacaaa tccccctttt gaaaataagg cttggggtct
    28141 aagtttagca ataatagcaa tcgattgaag aacgccaacc cctactttaa agacatctaa
    28201 catattttgc catgaaaaat agcgacgtag tttcccagta gctatagagt gaaatgtgat
    28261 atcaagtcca gactgattaa tttgttcatg ttctatgcca tttttatcac caatataatg
    28321 tacttcccaa ccatctttga taaatttggg gattaatatt aggtttagtg ttacatgacc
    28381 taccgtacca ccacctgtaa aaacaatttt tttccccata gttactctcc tctaagactt
    28441 tcgaaagtat caatgaattc atcaccacgg acttcgaaat tcttatacat gtcccatgat
    28501 gcatttgcag gacttagcaa gataacatcg ccctgttgtg ccacctcata agctttatgt
    28561 accgcatctc taacatctaa agcatcgcta taagttactc ctgctttttg tgcagcacgt
    28621 tttactcgag atgccgattc ccctaaaaca accatatgtt taagtccagt gatatctggt
    28681 atcaattcat caaactcatt accgcgatca agacctcctg caattaggat aactttagta
    28741 ttatcaaagc cagataatgc tttttgagtt gccaatatat tagttgactt gctgtcgtta
    28801 tagaaactaa taccatgaac cttaccgagt gattgcaagc ggtgtttaac acctccaaaa
    28861 ttgcttaaag tttctctaat aacttgatta ctgataccag ctagtttagc aaccgcaata
    28921 gttgctagag cattctctac gttatggctt cctgggacac caatgtcatc tactgacata
    28981 atattctccc ctttatagaa aagttgcttg tcttgtacgt aagcaccatc aactttttcc
    29041 gtagtagaga aaggaacgat tgttgcttta gtagttttag ctaactcttt agaaatacct
    29101 tgattaaaat taagtaccaa aaaatcagat gaagacattt gattttggat attccatttt
    29161 gcagcaacat aatcttcaaa agacccatga taatctaaat gagttggcat taaattagta
    29221 attactgcaa tatgaggacg aaattcctta actcccatta gctgaaaact tgataattcc
    29281 ataactagaa tatctttatc atccgcagcc tgaacaactt cactagcagg aaagccgata
    29341 ttcccagcta acaaacctct ctgacctcca gcatttaaga cttctgcaat catcgttgtc
    29401 gttgtcgttt tcccgttaga gcctgtaata cctattagct gagattctga aactaagtat
    29461 gctaattcca cttcagtcaa aacagggatt tgtttttcta atgctttttt gaccatagga
    29521 ttgttataag gtattcctgg atttttaatc atgtaacaaa aatcctcatc taacaattct
    29581 aaaggatgac taccacaaac cactttaata ccctcttcca acaaagactg tgctgttgga
    29641 ttttcatcaa atggtttgcc atcattaact gtcactattg ctcctaactt agctaacaaa
    29701 cgtgcagcgg cttctccaga tcgtgctaaa ccaaggacta aaactttttt attttcaaat
    29761 gttgttattg ttttcattac tcgtcccatt ctatcattat tatccctatt attttacctt
    29821 taatcagtgg ctttttcaag gtaaaggata tgtctctaat ttctcaaaac aaaagaactt
    29881 aggagacaaa atcctaagtt cataagctat ttcttctttt ctgacaaatt caatgtttct
    29941 ttaaatgctg tatatcctag aggaatcatt ataagcccaa tatacaattc acctggcaat
    30001 tgaaggattt taataaaact cataactgcc aagacaagta ataaggctac caaacctact
    30061 aaaccaatag cgttcaaagt attacgtact gtttttggtg ccataaaaaa gtaatataag
    30121 gcaatcaaaa gggcaacaat taagtaaaac atagaactcc tttcttatta atcataaata
    30181 agaaatactt tggatattat tcagctgatt ttttcttttt attagctttg tcacgtgctg
    30241 ctttatttaa aatttgttta cgaagacgaa tagactcagg agttacttcc atgtactcat
    30301 catctgctaa aaattcaagt gactcttcta aagtaaggat acgaggcgtc ttgatgactg
    30361 cagtttgatc tttagttgct gaacggacat ttgtcatttg tttagcagtt gtaatattga
    30421 ctccgagatc attatcacga gaattctcac caacaatcat tccttcataa acttctgtac
    30481 ctggatttac aaagatagtc ccacgttctt caatacgcat aattgaatat gtagttgctt
    30541 taccattttc aatagaaacc aagacaccac gatgacgacc accaatttct ccttgaacaa
    30601 ccggtagata ctggtcaaaa gtatgattca tgataccata tccacgtgtc attgaaagaa
    30661 actctgttga ataaccaatc aaaccacgtg caggaatcaa gaaaatcaaa cgcgtttgac
    30721 cattaccaac catctgcata tcaagcatat cacctttacg ctctgacaaa ctttggataa
    30781 tagcaccctg atattcttct ggagtatcaa tttgaacacg ctcaaacggc tcgcattgaa
    30841 caccatcaat ttctttgatg ataacttctg gacgtgatac ttgaagttca tatccctcac
    30901 gacgcattgt ttcaatgagg atagataaat gtaattctcc acgccctgaa accgtccatt
    30961 tatctggcga atctgttggg tcaacacgca aagaaacgtc tgtttgtaat tctgctaaaa
    31021 gacgttcttc aaccttacgt gacgtaatcc atttaccttc gcgacctgca aatggtgaat
    31081 tattcaccaa gaaagtcatt tgaagtgttg gctcgtcaat acgtaaaact ggtagtggtt
    31141 caatagcatc tgtcggagtt actgtctcac caacgaagat atcttccata ccagaaacag
    31201 cgattaaatc acccgcttta gcctcttgga tttctttacg ttcaagtcca aagaaaccaa
    31261 aaagttttgt tacgcggaag tttttagttg taccatcaag ttttgaaaga gtaacttgat
    31321 ctccaacttt gacagtcccg cggaaaacac gtccaatacc aatacgtcct acaaaatcat
    31381 tgtaatcaag aagagaaact tggaattgaa gtggttcttc cgagttgtca actggggctg
    31441 gaatgtgatc aatgatagta tcaaaaatcg gtgccattgt tttttcttga tctgaaggat
    31501 catctgacat tgaagatgtt ccattgatgg ctgaagcata aacaacaggg aaatctagtt
    31561 ggtcatcatc agcaccgagc tcaatgaaga gttcaagaac ttcatcaaca acctctgatg
    31621 gacgagctga tggcttatca attttattta caacaacgat tggaattaag ttttgttcaa
    31681 gagctttctt caaaacaaaa cgtgtttgtg gcattgttcc ttcgtaagca tcaacgacta
    31741 aaacaacacc atcaaccatt ttcataatac gctcaacttc accaccaaag tccgcgtgac
    31801 caggtgtgtc cataatattg atacgaacat cgttgtatgc tacggctgta ttttttgcaa
    31861 gaatggtgat accacgttct ttctcgatat catttgaatc cattgcacgt tcttcaagct
    31921 ctttacgctc atcaagagta tgtgattgtt ttaataattc atcaacgaga gttgttttac
    31981 cgtggtcaac gtgggcaatg atcgcaacgt tacggatatc tgttcttaaa tttgtcatgt
    32041 tttcctctat tttataatgt aattctgtta acttatgaat tataacacaa tttttcttaa
    32101 aaaacagaat ttacacaagt gtaaatgttt tcatattaac tgcctatttg tgataggaaa
    32161 acttttcatc atttcattat ttatgtcatt tattttctac ttttttcaat ttaattcatt
    32221 tctacaaaaa gagaagagag ttattaatag taactctctc cttagtatat tacttaactc
    32281 tacccgtcca gtaattgaaa ccgtccttta acacgtaaag ttgattaaaa ccttcttttc
    32341 tgagtaacaa aacaatcctt ggaatagact gaccacggct tgcgtcatat aagaggacag
    32401 gtttatcttt gcgtaaagcc gataaggcaa ctttaaattg acttgctgga atgttacgtg
    32461 ctccgagaat atgctttcta tgaaaagcac cagcttctcg gatatcaatt aactgtcctc
    32521 gtgacatttc tttttgaaaa gactcgttat ctaaaaattt agctgcccta cgaaccctcc
    32581 aataattcca agatgcccaa gcaacaaaag ccagaagaat aacgataatt aaaataacag
    32641 acatatccat atcttatctc cttaacttgc ttgttgattg gctaagcttg ctgcaccaat
    32701 aataccagca tcattaccta gttcagcaat cttaatttta gttgactttt taacttgtgg
    32761 gaaagcaaat gtgacaaagt atttctcaac gcgactacgt aaaaattcac ctgctgctga
    32821 gacaccgcca ccaataacca cagaatcagg gtttaaaata tttgaaatat tagctgctgc
    32881 cagtccaagg taacgtgata cacgttcaac aacagaatta gcaaatttat ccccatcttc
    32941 tgctgctata aaaatatctt tacttgtaac agtatcaccg ttgtcaatcg ctgctttaat
    33001 ggcagacgaa ccttcatatt gttctgcgag ttgacgtgct actctaacaa cacctgtcgc
    33061 tgatgcaact gtctcaagac agcctttgtt accacatgtg cacgtaaatc cattttctgg
    33121 atcaacaatc atatgcccaa tttctccacc tgctcctgca acaccatgga tgaggttacc
    33181 atctgcgata acacctccac ctactcctgt tccgagggtt acgaaaacaa catcgggatt
    33241 attggcacca gcacctaccc agcgttcacc aagtgctgca acattagcat cgttatcaat
    33301 aaaaaatgga attccaactt ctttttcaat aaccgaacct acttcttgag tatcagccca
    33361 atttagatta aaagcacctg ttactgtttt actagttcta tcaacagctc ctggagaacc
    33421 cataccgata ccgagaaagt catcttttgt taatccatag aggctcaaac gatgtttgag
    33481 agattcaacg atatcagaaa cgatatgtct tccgttttct aaagtattgg tctcaattgc
    33541 ccatttttct tgtacttctc cctcaagcgt caagatacca aatttaatgg tcgttcctcc
    33601 gaggtcaatt cccaataatt tcttactcat tttttatttt ccttctccaa ttctagtctg
    33661 tgctctcttc ttaaaatcag ttctgctttt aaatagtcat ctctacttat cagaccatta
    33721 tcgtagagac gttgcaattc aatcttcata acttcgatat cataaagacg tttaccaata
    33781 taaacataaa taccaaattt tttcaaaaga ttctgcacat cgaataaaac tttcatatat
    33841 gaattttaac agaaaaccat tcaattttca agaaaatccg caaccgcttg ctaattgtta
    33901 ttatgacgtt aggctgacta tctataatag aaatcataat ctcttttcta ccatttttcg
    33961 aatataaata taaactataa aaaggatttt atgattatgt ctaccatcta ttttatttct
    34021 ttatgcatga gttttatctt aagttattat gatataaaat accaagagta tcctattttt
    34081 ctatggatat tattcaccat ttcaacgata attctcacac cgattacaaa agtttctatt
    34141 gttttatgtt tatttgggat tttagctgaa gtggttgata ttaatatcgg aagtggagat
    34201 ttcctttatt tagccacaat tggcttatct ttacctcttc atcaaatgct ttttattatt
    34261 caaataggtg cttggttagg aattatttat tgtttagtta tgagaaaaat gaaaaaaacg
    34321 atagcttttc taccgttttt aagtatcgct tatataatcg tcaccagtta ctctctttta
    34381 ttctaatcac tttaaattat caggaaagtg ataaagttgt gggtactggt cgcattttgg
    34441 atttttagga tgacaaattg cacgaccaaa ataaatcatg gattgatgag cagctagcca
    34501 ctcttctggc ggtaaaacct ccatgactcg tttctcaatt tctagtggag aagccgactg
    34561 tttgcaaatc tggtggtgtt tacaaatccg agtgacatga gtatctacgg caaaagccgg
    34621 aataccaaat ccaacactca tgaccacatt agccgttttc cttccaacgc cagacaaact
    34681 ttctagctct tgacgagttt gggggacttt cccatcaaaa tgctctatca attgtttagc
    34741 gcattgattt aagaatcgag ctttattacg gtaaagccca attttagaaa tatagggttc
    34801 aatctctttg ggatcagctt gtgccaaaac taaaggattt ggaaagcgtt caaaaagggc
    34861 gggagtgact ttattaacag ctgcatcagt tgtttgagct gatagcatta cagctaccaa
    34921 aagctcaaag acattagtaa agtctaagct aggtttagca tctggaaata acttaataat
    34981 ctcacggata atatatcgtg attttgcttt tgataacatt ttattcctta tctttctttt
    35041 ttatcattat atcacgatat ctttcaaaat aaagaatatc agaactgagg tttgcaatcc
    35101 attaacccta ttttattttt ttagtaaaaa ctcatgctaa ttgtcttttg agttgttgat
    35161 aatcaatttt tccagatggc atcaaaggaa ttttagatac aatatgataa tgcttaaacc
    35221 catacttctt ttcaaaatgt tccgataacc acttcaaaca ctcttgttgc atctgatttt
    35281 caattaaaac aatacagcaa tgtgactctt gtccatgact ttgactctct tttgtaaatg
    35341 ctactgcatc cttgatataa gggcatgttt ttattttttc aattaatcta ggtaaataga
    35401 gcttaatgcc cgattgattg caccaatcgt caccacgacc aaatagatat aatttgccat
    35461 ctatcagttc accataatca ccagcacaat attcacttga aaggccacag atactatacg
    35521 gtgtttttac aaaaattctt ccctctttaa tcctaacagc tacatttgga acgatttctc
    35581 caacatactg tggcttatct cggatatcac gaccatcgta ccatgacaca taattgagtt
    35641 cactagcacc atagaagatt ttcactgaca ctttaggatg ctttatatat aaaccttcca
    35701 gtaatgagtc agagacatat tgcgaagagg taataatagt tttattgtct aacgcagtct
    35761 ccttgctata ttgttccact aacttgagat aactagggag cagatataga tgcgttacac
    35821 cagttttttc ccataaagtc tgccagtatt ttacagaatt tttctgtgtg acaacaagcg
    35881 ttccccccag taataaaagg gatagcgcta aatttaagtt accagtaaat gaaaaatcac
    35941 cttgaataaa cagtctgcta ttggaagtga ccgaaaaata agcattctga atgctaaaaa
    36001 aatcagacca cgaagcgaag ctcctccaca atagtttagc atcagctgta gtcccagaag
    36061 aaagaacacc aaaatcagct agttgaggag catactgaac ttctttttct aatcgactta
    36121 tttcattatg agtaaggtta ggtttacaaa taataggaag agctttttga cataactttg
    36181 aaacaaatct tactaattgg ttaagcagag agtgtgtgct aatcacatgt ttcctattgt
    36241 cttgtgacgc catatcttgt ctgactaagt tataaaattc tccataactg acctgtaaat
    36301 caccatcaaa taacttttta tcgctattgg ttttaactat tgttttcaat gattcaagca
    36361 ttcttaactc ctaagtactc tattgatatg gccattccga ctcctcctgc ccctgcaatg
    36421 gcagttagac ccataggttt atttttatat tttaatgcct gcataagatg aaggatatta
    36481 ataattcctg agcaggcata agggtgtccg taagctaatg cccctccaaa aatattgaat
    36541 ttttctctct cttcaggata ataatgatta aataaagcat caatcgctgc aaatggttca
    36601 ttccattcaa ttgcatcata atccgatatt ttagtatgag tttctgttaa tagtttttcc
    36661 gtagccgtgt gaaccaattc tggactaagc ttgggatctc ctgctacttc tacaatgtga
    36721 acaatccgga attctgtttt ctgactctga agcgttagaa atgcagcagc atcgtgcatt
    36781 aaacaaacat ttccaatagt gagcaaaggt gaattttcca tcaatcttgg taatttttga
    36841 aaaaatgctt cttttagttt tctaacgcct tgatctcgca tcccttccat tggtaagatt
    36901 acctcttcta aatagccacc ttgtttagct gttaaggcgc gtttatggct caagaatgcc
    36961 aatttatcta acatttctct tctaaaacca tatttttgac agactctttg tgccccttct
    37021 aacattacag tttcagcata agagtcagga gaaaactgag caacggtata ttctccgtta
    37081 cgattatctt ctttagcgta acgtctcata ggttgaagag aactactttc aatcccccca
    37141 acaagaactt tttcattaat accggcactg atttttagat aaccaaaaaa caaggcagaa
    37201 cttgatgaag cacactgcat atcaatcgtt tgtactggaa tataggattc ataatcagaa
    37261 aaaagagtca tcaaacgacc aatattgccc ccagtaccaa ctgtgttccc acaaataata
    37321 ctatcaatat tagattctga ttctattttt tttatttgat ttaagaggtg tgctcctaaa
    37381 agttctggac ggtaatgttt aaattgcttc ccttttatac cgataggtgt tcgtagccca
    37441 aaaccaatat aaacatctct attcttcata tgtccccctc acacataata tttgatgctg
    37501 aaaaataatc actgtgttat ccactacttg cactgagaat tcttgattca cccttactgg
    37561 agataaaaaa gtcaaatgaa tctcctttat ttttccattt ctaacttcca ttattttttc
    37621 caataataga gccgcaggca caatagctgc ttccccttgg tgaaaaggat ttttatctcg
    37681 cactaagcta gtgaattttt gacattcttc tattgaaata agactagcct tctcttccct
    37741 tgcaggacag tcactggatg ctagtgacat tggcttaagt tttggtaaat taagcatcct
    37801 caaatggtaa atccattgct gatcagttac cgttacctca aggattcgct tagaaatcca
    37861 tttaatatta tcagatgagc catcctctct ttgatacaaa ctaagttcaa caattacaaa
    37921 attaagcggc aaatctcggc acatagctat cgctttttta aatagttttt tcatcttatc
    37981 tcccgtcaaa atataagacc agtatatcat aattttttat attgttaact tattatatag
    38041 actaagttta caatacagtt tttttatgct attattaatt tcgttaaagg aggctatcat
    38101 gtcatttcaa acaaattata tccatttggc tgacgaaata ttatctggaa agactagcat
    38161 ttcttatgaa caagcgctgg aaattctaaa tagcgatgag aactggtggg aaatctacgc
    38221 tgctgcctta taccttaaaa atcaagttag ccgaaataat attcgcctta acgttttatt
    38281 aagtgctaag caaggacttt gtgcagaaaa ttgtggttac tgttctcaat ctaaagaaag
    38341 cactgctgac attgataaat ttggtctgct tcctcaaaat gtcattctaa aacaggctat
    38401 tgtcgctcat caaaatggtg ctagcgtctt ttgtattgcg atgagtggaa ctaagcctag
    38461 caagagagaa attgagcagt tatgtcaagt catccctgaa atcaaaaaaa gccttcctct
    38521 agaaatatgc cttaccgctg gttttttgga tagagaacaa cttcatcagc taaaacaagc
    38581 aggaattgac cgtatcaatc ataacctcaa cacccccgaa gaaaactacc ccaacattgc
    38641 aacgacgcat agcttcaaag atcgttgtga tactttagaa agaattcaca atgaagacat
    38701 tgatgtttgt tctggattca tttgtggtat gggagagagc gatgaggggc tcatcacatt
    38761 agctttcaga ctaaaagaac tgaaccccta ttctatccct gtcaattttt tacttgctgt
    38821 tgaaggaaca cctcttggaa aatataacta tttgactccc attaaatgct taaaaattat
    38881 ggccatgttg cgttttgttt ttcctttcaa ggaattaaga ttaagtgctg gacgggaggt
    38941 ccattttgag aattttgaat cattagtcac cttacttgtt gactcaactt ttttgggaaa
    39001 ttacctaaca gaggggggtc gcaatcaaca taccgatatt gaattcttgg aaaaattaca
    39061 actaaatcat actaaaaagg aattaattta aatgaaaaca agaacaacta cctatattgc
    39121 actcatggtt gcactactca ttgttttagg ttttatccca gggatcccat taggtttcat
    39181 tccagtccca attgtcttac aaaatcttgg tgttatgctg gcaggtgctc tgcttggtag
    39241 taggaaaggt ttcctagctg tggctatttt cttgctctta gttgctattg gtgcaccatt
    39301 ttaccaggag ggcggtcagg tctcgtaacg ctgtttgggc caacagcagg ctatctgctg
    39361 acttatccat ttgcagcttt ttttatcggc ctagggcttg agaaagtgaa aacaacaaaa
    39421 ctctgggttt aattccttat tatttggata tttggcgtac tattgattga tatttgtggt
    39481 agcatcgttc tttcctttca aacttctctc cccttaacta aatcactatt ttctaacctt
    39541 atttttattc ctggggatac cttaaaagca tccatttgct tgattattta tcgtaaattt
    39601 gcagacagat taactcatct atacaactaa ataaaagaca gtgcccttag gtactgtctt
    39661 ttatttatag agagtcatta tcctacacta acacatttta gtcattaatt aacgtcaaaa
    39721 agttttcaat cattttcatt ccatcaatac tcccgattga ctcaggatga aactgcatcg
    39781 catatacctt taagctgggg caatggaatg ccatgatttc attatcctca tttgatcgtg
    39841 caataatagc aatattctct ggtaaatcag tcactaccaa tgagtgataa cgcataactt
    39901 gggttggact atcaattccc ttaaacaaac tagtatggtc gtttattgtt acccaacttt
    39961 gtttaccatg cataacgtga tttgctaaat ttaatcgccc acccaagcac tcagcaagcg
    40021 cttgatgccc caaacagata cctagaatag gtttctgtcc tataaactga ttgattaatt
    40081 ctaatatttt cccagcatcc tttgggtgtc ctggtccagg tgataagacg atagcttctg
    40141 cgctctcagc taataaaaaa aggttaggat catcattttt tatgacgaat atttctttat
    40201 agacacttaa atattgcttt aaattatagg taaaagaatc gtaattatct accaatagta
    40261 acattttata gacctttcct tgtactttga cctcattata cactaattgt aaaatataaa
    40321 aaaggcttag aggaataact attccaacct ctaagcctca cagattatgc cttttgaata
    40381 gcctaaattt tctagacctc catcatgccg gcttgtaatt ggtacatacg ataataagtc
    40441 cctttttggt ctaataaaga ctcgtgattc ccactttcaa ttatctttcc tctatcaaga
    40501 acatagatac aattagcatc ttgaatggtt gataagcggt gagcaatggc aattgttgtt
    40561 cgaccttgac gcatttttgc taacgaatct tgtacaatct gctctgtttc cgaatcaata
    40621 tttgctgtcg cctcatctaa gattaaaatt ttaggtttac tagccacagt tctggcaaac
    40681 gctaatagtt ggcgttgtcc agttgaaaaa cttgacccac gttctgaaac agctgaatcg
    40741 tacttatctg gcaacttctg aataaattga tcagcatcaa caaactctgc tgcgtcctgt
    40801 acctcttgat cggtgatgtc ttggtacatt ttgatattag atttaatagt gccatgatat
    40861 aaaaaggggt cttgtaagac gagtccaata ttctttcgca attgttcttg tgagtaatcc
    40921 ctaatatcct tcccatctaa taaaacttgt ccagattgaa attcgtaaaa cctcataaag
    40981 acattaataa tagatgattt tccagaacct gtcgctccta caaaagcaat agtttcacct
    41041 tttttaacac taaaagaaac attatcaagg atttgttttt ttccatcata tgagaatgat
    41101 acatttttaa attcgatatt tccttctctc acaaaagctt ctgtattttt ttggcttggt
    41161 tcaaaacctg tttcatcaat cagatcaaac acacgccctg ctgataccat tgatgtttgt
    41221 aaggttgaaa aattttgcgt tacttcaatt aaagggtcaa atagacgatt aacgtactga
    41281 ataaaagcat acattaatcc tgccgtaaga cctcctttaa ctcctgtaaa tccaaaataa
    41341 gacataagaa cagcatatgc taggagtttt aaaagagaca tcgccggtct taagaagaga
    41401 ctatcaagag ccatagaacg attggcatac acaacatgct ctttgttaat ttcctcaaat
    41461 tcagtcttca agcgctcttc ttgaccaaaa gcctgtacaa tgcgaattcc ttcaatactt
    41521 tctgataatt tactgttgat atcactaagt aaacttctcg ttttagcaat gacagtgact
    41581 gattttttcc gatagacatt cactaatata aagataacag gtaacaaaag agcgacgagt
    41641 cctgttagtt taatgtctag catcaacata gtgtacagag taactgtaaa aataaatatc
    41701 gccgagataa aacttgataa aatacccgaa aacatatcag atattgcttc agtatcatta
    41761 gtaatacgtg acacaataga tcctgccggt gtcctatcaa aataagacat gcctagcctt
    41821 tccatattag caaaagcatc tctacgaata tctctaacaa tactataaga aacacgcgca
    41881 aaaaagagat tcccaaaata ttgaattaag gtctgcaaga catacattga ataatatccc
    41941 actaaaataa gaactgctgt ttgattaaca tttgtcagat agtgatcaat aaaatgtgaa
    42001 gcaattaaag gaataatatt tttaacaaca gtcgtcaaca ataagagaga tagagctaat
    42061 actgtaaacc atttataagg gcgtaaatag gagattaatc tcttaaagac ttgccattga
    42121 ttagacttca tcaaatgctt cctccatttc taattgctgt gaggcatacg tttcagcata
    42181 ccaaccacct ttatttagca actcttgatg ttgacctcgc tcaatgactc tgccgtctcg
    42241 cataactaag ataaggtctg cgtgcacaac agctgataaa cgatgtgctg aaataatagt
    42301 cgatttccct tgacgattcg ttttaagatt ctcaacaata gcatgttccg ttttagcgtc
    42361 cactgctgat agagaatcat ccaaaataag aatatctgga tctaaaatca tggcacgact
    42421 catcgcaatc ctttgttttt gtccaccaga taatgagact cctttttctc caattagagt
    42481 ctcaaatcct gctggcatct gttcaatgtc atcgtaaaca tgtgccaatt tagttgcttc
    42541 tttgacagca ttgatagata gagttggatt tccaaagcga acattttcta aaatactggt
    42601 agcaaataaa aactgatctt gaggaacata accgattagt tgacgtaact cagacaatcg
    42661 ataatcacgt atatcatgtt tatttaaagt aattttcccc tgagtcacat catgttcacg
    42721 tagcaataac ttaataagac ttgtcttccc tgatcccgtt tgacctacca aacctaaggt
    42781 ttgacctttt tctaaggtga aatgaatatc ggctaaggtt tcctcattgt cgtatctaaa
    42841 gaaatcaata tcatatctta atgttccatt gacaacaggt ttgataggat ttaaaggatc
    42901 agttatatcc gattcttgct caagtagact attaatacgg ttataagaaa cactaccacg
    42961 ctgtaccata ttgaacaaga aaccaatcgc catcaagggc cataccaaca tatctaaata
    43021 cgtcacaaat gttaccaagt caccaacagt aacttgacct tttgaaatca taaaagctcc
    43081 catagccaat gttaatacgt aggaggcacc tataaaaaga agaactaaag gatcaaacat
    43141 gacatcataa gtcatggtcc gcatgttctt cacgaaagtc atctgattaa tctcttgaaa
    43201 agaagcaatt tcttgttctt gataaccaaa tgatttagtc actttgacgc cagagacact
    43261 ttcttgcact ttattattta attctgaaaa ggctgcctga gattctttga aggtttcatg
    43321 ggtttttcgc cccaatttac tagttgctaa ggccattaag ggcaaaggga taaccgcaat
    43381 taatgtcatt tgccacgaaa tagtaaagaa catggtgata agcgttacta atgctgtgat
    43441 agaggcatcc actgctgaca taactcctcc tcctgcaaga cgtgttagag aattgatgtc
    43501 gttggtcgcg tgcgccatta aatcacctgt acgatatttc tgataaaaag aaggagacat
    43561 ttttgtaaaa tgttcaaata aacggtatct gacaacttgg cctaatttgt aagaagtccc
    43621 taaaatatac atacgccaaa tataacgcag cccatacata gctaaagctg acaaaaccaa
    43681 acctaataaa ttccatagta attgtggtct tgttaatttt ccagttgtaa tagcatcaat
    43741 aactgatccc atgattttag gagggataag atttaacact gcaaccaaac ttaatgacaa
    43801 aatcccaata agatagcgct tcttttcttc cttaaaaaac caccacaaat tttttataat
    43861 agacatattt ttcctttctc aaaggttgaa aaacatcata attaagtcag aagaccaaat
    43921 caatcagaag acataaaaag tgttttaaaa ttctcattta acaactctct attatgcctc
    43981 tataagctta ctttcgtcaa ctaaagtgat cctttttaaa tagtaaaagg cttgaacaat
    44041 atcatagcct ttacattatt ttcattttct aatagttaga agccaacctt ttatgtttcg
    44101 ataactccat agggcgaaag gaccgcctaa ggtatttgtc catagatcgt cgatttcaaa
    44161 aacccgatta gcatctatta aaatatctaa aacaacttga gtacactcta tgaaaagaga
    44221 catcaagaaa gcaagtaata taacgctttt atatttccgt aaacttggct ttagggatag
    44281 taaccctata atgagaggaa acagcagtaa aatattcact acattttgac caataaccca
    44341 aaaaatctct ttaaagctag ttagctgatc taaactacga aaagaattaa atggtactaa
    44401 aagagctaca attcttccga agcgctgaac attcggagtt gataccccct caatcataat
    44461 ttgaggccca aaacacatcc tgacaataat aagacaatag ataaccacta acatccaaac
    44521 gaaacgacga ctaatctttg tcaattcccc atcttcagta aataatcttt taagcattac
    44581 cgccctacct tcgctttttg acgatcgcgt ttcattgtgt ttgaacgtaa ttgtccgcaa
    44641 gctgcatcaa tgtccgtacc atgttcttga cgaacaacac aattaactcc atttttcttc
    44701 aaaacgtcat agaaagcttc cacacgctct ttaggactac gactatactg gtcatgttct
    44761 gagacaggat tgtaaggaat taaattgaca taagataatt tgcgaatctt tttcgttaaa
    44821 tctgctaact cttgagcatt ttctggggta tcgttgacac catttaacat aatatactca
    44881 aatgttacac gacggttagt cgtctcaata taatattcaa tagccgcaaa caacttttca
    44941 agagggaaag aacgattaat acgcatgata cttgatcgta attcattatt tggagcatga
    45001 agagatactg ctaagttaac ttgtacacct tcatttgcaa attcacgaat tttatgtgct
    45061 aaacctgaag tagacactgt aatgtgacgt gcaccaatag ctaatccatt atcatcatta
    45121 actgtacgca agaattttaa tacattggta tagttatcaa agggttcacc tattcccatg
    45181 accacaatgt gactaacgcg ctcaccttgt ccacgttcat caaaatattt ttgaacaagc
    45241 ataatttgag cagtgatttc gccattgtta agatcacgtt gtttctttat taaaccactt
    45301 gcacagaaag tgcaaccaat gttacaacca acttgagtcg ttacacatac tgataaacca
    45361 taatattgac gcatcaaaac cgtctcaatc agcatgccat caggtaattc aaaaaggtat
    45421 ttaacagttc catcagcaga ttcttgaaca atacgttgtt ttagaggatt gacaacaaaa
    45481 ttctcattca aaagtgcaat aaaatctttg gaaatgttag tcatttcatc gaagctttgc
    45541 acacgctttt tatagagcca gtcccagatt tgtgaagcac ggaatttctt ttctccatgt
    45601 tcaatagccc aagctattag ttcatcgcga gttaagctat aaatagaggg tttatagtcg
    45661 ggtgtatcag tttttttagg cataatattt tctttcttca tggtggaata aacgattaca
    45721 gtttatcctt tttttatttt ttcttacgaa taacaaaatg ctgctttgca gaggtcattt
    45781 ccttatcttg ctcttcttta atatgacgtt cttgacggcg ttttgtgtct ttacgctttt
    45841 tacttgtaaa atgagcattg gcatttgact gggaacgatt atttctagtt tgtgactttg
    45901 atgattgtga tttggatctt gacttacgac gcttatgagg tctttcatca tcaaatttaa
    45961 tatctctagg atttggattc tccaacacaa aataagcaca accaaagtta caatactctt
    46021 taatataatc ttctaaacgt gaaatccggc tattttttct aatcgtacta gcatctttgt
    46081 aaaagcctct tagcctcaac tgctcgttac cccaatcgcc aacgataaaa tcatatttta
    46141 ataaaatctc agaaaaacgt tgtccaaaga cagtcacatc aaaagctgat ttttcattaa
    46201 taacaagttg aaattcaata tcatcacttt taacgatatt ttcaaagtga ataaactgtg
    46261 ggccaggata cttattatag ttaagcatct ctggtgtcac ttcttttcgc ataatactcc
    46321 tttctctaaa atcattgaga gaaacgacta gcttctactc tttatttatc tatttttcat
    46381 ttatagtgtc atatttatta aataatctgc taccacatct ctgagtaatt caggaaaaag
    46441 caagtctact ttttttgttt ttaaacactc aaaatacgat gcaaaataat actgatcaac
    46501 gattacaaga atcacttctt ctttggcatc aatatctttc tcattataca ctatttttcc
    46561 atttttgtaa tcaaatcccg aatgtaaaac ttctccaaag tacttgccac gaaagcccat
    46621 accatgaatc ttttgttgcg ctaaaaattg accttgttga taaatagttt cataaatctc
    46681 taaaagttct tgactactca ctgtcaattt agcgagacgc atttgatgag gtaatgattc
    46741 atgcagattt ttcctcgaaa agtctttttc aaatggctga acaattaagc cagaattgat
    46801 aatagcaaca tctgcacctg cgtaaaacag catcgcatcc atgactagtt gacacgattc
    46861 ttctaatgat aatggtgctg gaaaggatat taaactttct tttctaagct gttctttacc
    46921 ttctgactca atctccttag cccaatcact gtccgaagga taaccagtta gttgttttgt
    46981 atcatatgtt gagattaaaa tatcttttag tgtatgatta tcaaaagtta tatcaatact
    47041 cccaacaaag cgaccatatt taccagctgc tgcaagatat gtaccggtaa ttagctcccc
    47101 ttcctcaaat aaatggtgtg tatgtgcacc tataataagg tcaatttctg aaaattcttg
    47161 agcaatcctt gtatcaaaac gaatacctaa atgactcatc aaaatacgac aatttgcctc
    47221 ttttatctca tttatttgta aatggcattt aagacagtca attggatctt ctatcgtcca
    47281 tccattaggt tcataggttt tataataagg aaaagtataa gctaaaaaag caagcttagt
    47341 tccttgctgt gtctcataaa taatgtaagg ttttgcccaa ctcggttcga ttatgttatc
    47401 ttttagatta ccaacaataa ccgtaaaatc tgaatctttg tagacttggt ccaagtcttt
    47461 tttggataaa ccaactcctt cattattacc aatagtagca agctcaatac ccagttcatt
    47521 catcaattgg acattagcct tgcccgagct tgcttctgtc aaaggatggc ttttatcaat
    47581 attatccccc aagtcaagtg aaatcgtctc tattggctgc gcttgattgt tatgaaaaaa
    47641 tcgttttacc ttaggaaaat tctcaaaatg agagtgcaga tcatttaaat gtagtattct
    47701 tataagctct gtcatactat cattttagca ctttgatagt taaatgtcgc aaaacaaaaa
    47761 agccgagcag acgcccgaac tctttgagtt tattttgttt ttttcaaata atcaatagct
    47821 tgctgaacat tttgaactgg tacaattttc atcttcgtac ctaatcgctt agctgctgct
    47881 tttgcctctt gataattcgt ctgaaccttt gttttgcctt ttttagcatt tttatcgatc
    47941 ggattattag gaacaaagaa aatatccatt ccttttttag ccgctgaaac aactttcaaa
    48001 cctgctccac caatatcgcc aacatgccca ttttgctcaa tagtaccagt gcctgcaatc
    48061 ttacgtcctt tacgtaaatc ttctttaact aattggtcgt aaatcgctaa agtaaacatt
    48121 aaacctgcac ttggtcctcc aacaccttca gtattaaagt ccacaggaac atcagatgat
    48181 acttctgtat ggtctgtcaa accgatacca ataccatttt taccgtttga tagtttaata
    48241 actttaccaa ctgattcttt ctttttacct tgacttgtgt attgtacctt tactttatca
    48301 cctagatgaa gagctgcaac atatttgata agttgagaag aatttttgaa acttttgcca
    48361 ttaactcctg tgactgtatc agctaaatgt aatctatctt taaatgtcga atttttagct
    48421 aagttaagca cataaactcc tttataagta agagatactt gcttattagc taactttaaa
    48481 gcttgataga tggactcatt ttgagaagtc tccatgtaga attgattaat tctgagataa
    48541 tcgtcattac taaagccacc tgttgtctcc tctttactag ataattctgt aaaaggagtt
    48601 aaccaagcat aaagaacctg agctggcgtt gcttgactga cagataccgc tacaaaattg
    48661 tacgaaccct tagctttatc agccttttta ttcactttca ataccgaacg gatatcataa
    48721 gctccacctg gcatttccaa ataataaggt agcctcacca caaggcttgc taatactaac
    48781 aataaaaaag caaaacctat aatccaccat ttaagcagac ctagcaaact tttatgtttt
    48841 ctttttggat ctttattctt cactcatttt ctccacttct ctaaaaacac tctttggaac
    48901 aaaaggcttc acagaagcct taaaatgtat caattcacgg atgcgactag atgatatcgg
    48961 actcaaagat ggcgacgttg ataaataaac tgtctcaata ccatctgcca actgtttatt
    49021 aaaaaattcc aaattggctt cataatcaaa atcctggcta ttacgcagac ctcgaacaaa
    49081 atattttgct cccacttccc ttgctaagtc aacagctagt ctatcttgcg ccactaaaac
    49141 tgaaacattc ttaaactgac ggatagcttc ttctaacatt tttttacggc actcaatcga
    49201 aaagtaacct tgcttctcaa ggttatgaaa taacccaatg taaacatgat caaatagata
    49261 gctggcgcgc tcaataatat ctaaatgtcc attagtaaca ggatcaaatg aacctgtaaa
    49321 taaagctttc tttgtcattc gtcgtcatct ccacaataat acaaccataa aaagactacc
    49381 acaggaatca ggcgaacaat gtctattgcc tcaaaaccgt tagctatcca attgataaga
    49441 tttggcaaaa aagtaatcat tgccattaat agatacaatg gattttttgt tcttgataat
    49501 gaacaaactc cttcatcatt tttgaagtga taatgtttta gcaactcttc taaatagtga
    49561 aggcgcaaca acaaataaat caatatgact aagatgagaa taatcatcac taacttgtaa
    49621 tccataccaa agcctcctaa cgaacgtaga ccgttacttt agaaatgcca taaattttct
    49681 gtttccatat cccaaaagaa gcaatttctt caggtaaatc aactgatttg tccgtttcac
    49741 aaacaatcat gatatcatcg cctaataaac ccttgctatc catgatttgg atttgcttta
    49801 caatttcttc cttagcatat ggcgggtcca acaagaccaa atcaaattgt cccgttaatt
    49861 gttctaaagc acggttggct tctattttta ataattgaaa ttgctccgga ctcttagtca
    49921 ttgcaatatt ttcttgaata acgacctgcg cacgcctatc tttttcaact aggacagctt
    49981 ggtccattcc ccttgagatc gcctcaatag ctaagctacc actgccagaa aaaaggtcta
    50041 aaacacgacc accttcaaaa aaaggaccaa tcatattaaa aatagcgcct ttaaccttat
    50101 cagttgttgg acgagttgtt tttccatcaa gcgtttttaa aggacgccct ccaaatgttc
    50161 ctgctactac tctcataacc actattataa caaaaaataa ggtagagtta tcaaaaaaat
    50221 tgcttataac aaacagagaa gatagtatct cttttccttc ttttttgata tgataagcta
    50281 gcagtaagag aaacgaggtt cccataatga ttaaaaaatt attattaact ttaatagcca
    50341 tattatgcct tacaacgcta aaatcaaact taagtagcgc tgacaccatg actatttctc
    50401 aacaacaata ccaacacatg ataacaaata gtgacttaat cgccatcaca gataaggcct
    50461 tagtaagcaa aagtcaacaa tctgaagcga ctatttttat aggacgtcca acttgccaat
    50521 attgtagagc tttcttacca aaattattaa aatctcaagc cacacttcat tcaaaaattt
    50581 actaccttga tagtcaaaaa tacaaaggga aacgattgaa gagttttttc aaaaagcatc
    50641 atattaccac tgttccaaat ttagcacatt atcaacgagg aaaaatgact aaatatcttg
    50701 ttcaaggaag ccaagctaca cctcaacaaa tccaaacttt cttaagccaa aaataaactt
    50761 attgttaaaa tcagccaaac aaaactgctt ggctgatttt ttataatata ttttcgaagg
    50821 tatctttgac ttcttttggc cagacacttg actgtacttc tccaatatgt tttttacgaa
    50881 gcaagaacat agctaatcgt gattgaccaa ttcccccacc aatagtcaga gggaagaaac
    50941 cacgtaatag cgtcttatgc cactcaaact ctaaacgatc ttcatcaccg gtgagaacaa
    51001 cttgacgttt aagagcatct tcatcaactc gaattcccat tgatgatagt tcaaaggcag
    51061 ttccaagctg ttcgttccat actaaaatat caccattgag acctttaaaa ccattttctg
    51121 atggtgttgt ccagtcatca tagtccggtg cacgaccatc atgtggttta ccatctgcta
    51181 actctccacc aataccaatc aaaaagactg cgccatactc cttagcgatg gcgttttcac
    51241 gctctttagg gcttaagtct gggtattttt ctaccaattc ttctgtatga ataaaggtaa
    51301 tacgctttgg taaaatggat tcaatatcaa aacgagcttc cacagcaagc tctgttaggc
    51361 ggatagcttt gtagattttt tccacagttt cttttaggta gtccaaatta cgtctaccat
    51421 caggaatgac cttctcccag tcccactgat ctacgtaaac tgagtgtgtt ggatcaagag
    51481 aatcttcatc tggacgaaga gctttcatat gaacgaaaag accttctccc tcattaaacc
    51541 caaatcttgc caaagtatga cgtttccatt tagccaatga gtgaacgact tcaaattcag
    51601 cttcaggtat attaaggact ttcaccgata caggatgttc aataccagat aaattatctt
    51661 gcataccatc cccgacctga ctaagaatag gtccttgcac ttcgacgatt tctaacttat
    51721 caatcaaata ttgtgtaaaa gtatttttga caaaagaaat ctcctgttgc tgatgaataa
    51781 agcttttctt catatgttcc tccttaaatt ataaaatgtt agagctatta tactccttca
    51841 agtaacaaaa tggaagagga attgtagaat tattctgact tttttacaag tttttttctg
    51901 aaagtcatta ctctagaatt gtttttgcca tgacttgtcg caattccttg gtcattcgcc
    51961 gtggtcctct cacagtctta acgccgtgtt ctgctaaaaa tgctacagga taggtagaat
    52021 cttcaaattg ccttatcagt ttgagtcgca tataagcttt gacatctctt gctcctaagt
    52081 aagtacttgt tttaaggtca cgttctatca cttgacagac atagcgcaaa gcacgaatag
    52141 gtgctgtaat ataaataaac acataatctc ccaccgcaat ctttcctttt tgagtccaat
    52201 caatcaattg atgctctgca aactccttat caatatcgta aaatttagga ttagcaggaa
    52261 taatccaaaa ctctggctca gaatttgatt ttttattggg agaaacaagc ttacgactat
    52321 ctgataccaa tttaaaaatt tctatatcag acaaagtatt gtctaaaacg atagataccc
    52381 aagtcttttt agacatatga taagaaggat aaataccttc tttttgtaag agtatttcca
    52441 tatcttgagg attgacttta atattcaaaa cttcaacctc ttcttctgac agttgagctg
    52501 gaacattttc tagcttcccc atcttcaatg gaaaaagaag agcataccat ttacctgata
    52561 cacgataaga agcatattgt gggtgcttcg caaatggata atcaactgaa tcttcaaaag
    52621 ttgtagctat cttctccgct aaacgattac tttgatcttt ggtaaataat aaatccttac
    52681 aacaagaatc tgaaatatca cctaatatag ccatataagc cgatcgtact tcgccaacaa
    52741 acgaaccatt ataatttgct gctcgaagcg gtaaatattc ttcttcaagt gccgaatcaa
    52801 taaccttacc agctatcttt ccatccaatg aaatcgtgat aatagcttta aagtcattat
    52861 ccataaatgt ttgacaatag atatactctt ggtcagattt gataaatcca aactcctcaa
    52921 gcgatgaaaa aataaaacgt tttttccgga aaaaatctga ttctattgac atagtaacct
    52981 ctctattcac tgtacattat cctctattat gtcgttttaa gagttagaaa gcaagctcaa
    53041 agtcaaagat gaattctagt ttctttgtac aaaaaaagca tgttgccatg cttaaaacta
    53101 aactaattga tagcaccctc ttattaattc tctatttatg tttgggatga ttatcctctt
    53161 tatggacata aatgtacata acaatagaga gcataatagt aatgatagtc gtaattaaaa
    53221 taatcaatac ccagacagca tcaacactat caaaaggcaa cttaacattc attccataaa
    53281 agctggtaac catagcaata atggaaatac caatggaaat aattgttaag gtagttacat
    53341 tgtcgttaag gttattgctg aggacattat tataagaaga agaaagttct tggaaaacct
    53401 gtgaatttaa ggaacacatg ttagataatt gtcttgcttc aataatagca tcttgaagtt
    53461 gcatcttttc atttctagta ttctctttat cttgctcaat gttttgtaaa tcggtcagca
    53521 tttcaaaatt ttgcttagtt cccatcatta agtgtactgt tccactttgt aaatttgcca
    53581 gccttgccaa gttcgatttg tttggtcttt tacgcaaatc attgattagt ttatctcttt
    53641 cttcagaaat agtatctaaa acatcaaaat attgctttga gaaagcagtc actaatgata
    53701 ataagaggtg tttcggcgaa tctacctttt caacgcgatt aagtgctttt tttacaagga
    53761 taatattttt atgattagct aatacaaaca agccatcttc tttcaaaaat agagtcactg
    53821 gtatcactct cggcacatga cgaacatcat caacgtgacc ttgcgtatcc aaaagctggt
    53881 aaacaagtgt caaatgcccc tcaatatcac gtacgaaaga agactcgttt tcatcacgtg
    53941 cataagccag caattctgaa ggtaaaaatt tctcctcttg taaggataga tcgtcaacat
    54001 taattatctc aaatccaaaa tcgctcgaaa acatagaagc acctcactct aaacttgtct
    54061 acattatgaa acaaaaatcg agaaaaaaca agtagaaaac ccttttaaat ctaattaacc
    54121 ttatgagagc aaaacaaaaa aggactacta aaataacatc tagtactcct tattaatatg
    54181 ctagatagcc agcatcattt cccccatgaa cggtataagc cttctataat cttaacacct
    54241 gacagcgtca actgaggcgt catgatatga cttacccttt gcccttctaa aatcatttga
    54301 ctaacatgat aagcttcttt ctcaaaatca ctaaccatgt caacttgaat cgttcgcgca
    54361 ctagtatcat ttctcaccaa ttttgcatga gtggttttcc aaaaatgtgg aatgattaga
    54421 cgaccttcag ttccataaat aatcatttca tgcggtaaat ttaaacgtgt tgtcaagaag
    54481 atatctacga ggaccccatt tgataattgc aagagtagtt tggattgact atcgctttga
    54541 ccttttggaa aagtcgcagt cccactagca tgagttatag tcgcatcaaa taggtattgt
    54601 aaataagaca gggcataggg cgccataaaa tgcactgttc ctccacctaa ctcaagttct
    54661 ctaaaccatg ttacatggtc aatgtttggg taggctgttg tagatgagat ggagatcact
    54721 tctcctattt caccagaggc aagtaatttt ttaatcactt gtgtcatcgg aataaatacc
    54781 gatttttgtg cttccatcaa aaacagatta caactttctg ccaaagtgaa taattcattt
    54841 gcttggtcgt aagtcaatgt gaaaggcttt tctaccaaca catgttttcc cgctaacaga
    54901 gctgctttag caactttata atggtcttga ttaattgttg ccacataaat cacatcaatc
    54961 gactcatctg ctaacatatc ctctaactta tcgtaagcct ttgggagatg atatttgttg
    55021 gcaaaagctt gcgcagactc aagagttcga ctcgatacag ccacaacttc accatttcct
    55081 gctaagcgga ctccttcaat aaaacgaggc gccacctttg ccgttgacac aacaccatat
    55141 cttaccttta acataaaaac tcctttgaca ttgatagagt tattatagca taagaactag
    55201 caatttatct ttttagggat taatacaaat caatcgcatc tctgttaaaa gttagacaat
    55261 tttaatagca taatttgaaa gatttaaaat agttgaaact acacacccgc aaagggtgcg
    55321 acgaggaaca ttttctcgtg aagttgctga acttatattt caatccacgc acccgcaatg
    55381 ggtgcgacgt aaaacttggt gatgaagcaa cgtataaact tatttcaatc cactatttca
    55441 gctaattctc ccaaattccc tccataacag atacgatggt caatctctga agaatcataa
    55501 gcaggttgtg ttgctgtaca aagaacgatg gttgtatcca taactttatt aaaaaaattc
    55561 atcgttaaat taaacaaagt ggtgacttca ataggcagtg actgaacttc atctagaatc
    55621 acaacactat taatcaaact tgaaaaacgt ctcaaattag ccgattttgt tttaaaaagc
    55681 gtttggaaaa attgaaccat agaagtcaag actacttgac tatcccagct atcaataaga
    55741 taagctgaca ataaactgtc cttgtcatca tcttcattag cctgtttaac catattggaa
    55801 tggtgttcta gaacgccaag gtcacccgta acttttctaa tttcggaagc attttgctca
    55861 agaaccgaaa gaaagggcgt tatgtaaaaa aatcttgatt tgtcttgatg aactaattgg
    55921 tgaaacgcat agcgcatact aagattagtc ttgccagctc ctgtcggtaa atctaagcga
    55981 tagatccctt tggaatcccg tttacctctt tctttaacac gctcagcaat ttctgtccga
    56041 atagtattca actgattgtt tggtcgtcca aagctagcat atttttgttc aatggctgct
    56101 aaataagaat gttttagaga ggatcgctct gttttatcca taggacttat tttaagacca
    56161 taggcattta ctgtgtccaa aatatcagcg ttttttaaga gcgacaagta aagtctcacc
    56221 atacaagact gataataatc ccactcacta ttatcttgcc agtttaagga agacatagct
    56281 tgttggtaat tatcaaaagc tttatcaata agctccctta aatctttgta gccgtaatca
    56341 cacaattttt cttctaattt tatagcatac cctttaatat cttcgtgata gtgataatca
    56401 tctaagtctc tgttgatacg gtttttaaat ttattaaagc tataatattc agcatcgtca
    56461 gaacagtagc aaaaatcata catcccatga tgagccgaga tgacataacc caccatttca
    56521 ttgaatgtca ttctctcacc tttatcaacc cctcgacttt ttagatgagg cccaataata
    56581 gagcataagt attttgcccc tgcataagaa tgatcaacat gtcgatttgg attatttaat
    56641 aatttatctt gaaaggctcg atcagctttg cctagatcgt ggtatagacc aattaaaaaa
    56701 agcacatctc cttgaccaat tatagatgct tcctgtcgac taaaacaggc cacatgccat
    56761 gagtgctcat ctaaagactg ctttttttca gattcttgtt ctattttctt ttgataatgt
    56821 gccaacgttt ccttagtcat catcatcctc tctcccttat ccataactca tatccctcaa
    56881 tgatatacta acatttttga aaggaattgt aaacggatac aatagttaat acttccccca
    56941 aaaagcctct aaatgacttt ccaaaaaaac aagaatcttg acaattcaaa atccttgtta
    57001 ggagttctgt aaaaaagaaa ttatttttcg gctaggcgta gttcaactta ggaaacttaa
    57061 acctagctta attggaagat gttaaatttt tataacacct aacgctgaca atgctgataa
    57121 tatcgacctt tacatttggt acttattttt tgtcatctcg actagtaaac gtgtgttcta
    57181 aatcgccaag cattagtttt ttcagcacat acaacttttc ctttcattct tgcggtgata
    57241 ccatcaaaat gctaaattaa atttaacatt gacaacaaaa cacttgttcc ctaaacagta
    57301 tcaataagtc aaccgatcgg tttaaaatac tactgtctta tcgtatgtaa ccccctctat
    57361 ctagttgctg gattcaagta gtatgataaa acctacattt tatgcatgct tactaccatt
    57421 agaatggaat tgttatgacc aagttaaagt attcgttatt ttttctcatc tatcttgcac
    57481 tactatatct aactagtatc tactttcctg ctatttataa agtcctcatg tatcttcact
    57541 ttctatgtct attcattttt agcattatgt tcctttataa tctgattgtc attttaaaat
    57601 atatctttac gcataaaaag aagtagatta gcatatattt ttgtttgctc gttataaaat
    57661 taagttttta aggaatgtta aaactactaa acaaacccaa attgatagaa aatagaaaat
    57721 actactaata ttatgaatca ttacaacatc tctagaaaat catgatgtgc taaatcaaaa
    57781 gatgcatgtg tttttacttg aaaaaaattt tttatatgtg ataatcataa tgaataggaa
    57841 tcaaaaggag aacggaatgc taaattctga ttttattatt agtaagtcac ttgctaacta
    57901 cattcaccat cgtaggttag aagttggcgt gtcgtcaact gatttggcag aaatatccaa
    57961 tatgtctaaa tcagattggg aatcatttga aaaaaatggt ggggcaattc ctttgaaatc
    58021 taaggatatc atacttgact tattattttt ggagagattc cctaaagaga aagaatgtga
    58081 ttttatagat aaattatttg aggaagcaaa ggaaaataaa ctttggcctg aaaagattta
    58141 ccagactatg ggacttactc ctgctctttc atttattgct ggttgtgaaa tactttctga
    58201 tgatattaat aatgatttgg aggaattatc taaactacct aaagaaagtc atctgggaca
    58261 attagatact tccctacttt tatcactatt gcctcaacaa tttatcacaa aatatgatta
    58321 tgagtttgtt tataaattaa gcaaagtttt ggctca
//
LOCUS       DATRME010000005        57011 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRME010000005 DATRME010000000
VERSION     DATRME010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57011
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..617
                     /locus_tag="VBC23_000286"
     CDS             <1..617
                     /locus_tag="VBC23_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6842264.1"
                     /translation="QFDHIVSHIKHRTALKNHTLFFEGDYREKLFLIQSGHVKIEQSD
                     ASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAITDIEYFSLPMALYEEYSLQNINQ
                     MKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLATLLLQVPTERGHLPFPITTIEI
                     ANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNKNYFKKFIE"
     gene            627..1112
                     /locus_tag="VBC23_000287"
     CDS             627..1112
                     /locus_tag="VBC23_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6842265.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            1269..2750
                     /gene="guaB"
                     /locus_tag="VBC23_000288"
     CDS             1269..2750
                     /gene="guaB"
                     /locus_tag="VBC23_000288"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6842266.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            2826..3488
                     /locus_tag="VBC23_000289"
     CDS             2826..3488
                     /locus_tag="VBC23_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842267.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            3544..4410
                     /locus_tag="VBC23_000290"
     CDS             3544..4410
                     /locus_tag="VBC23_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6842268.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFMLGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(4422..5531)
                     /gene="recF"
                     /locus_tag="VBC23_000291"
     CDS             complement(4422..5531)
                     /gene="recF"
                     /locus_tag="VBC23_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6842269.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(5534..5887)
                     /gene="yaaA"
                     /locus_tag="VBC23_000292"
     CDS             complement(5534..5887)
                     /gene="yaaA"
                     /locus_tag="VBC23_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6842270.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            6126..7370
                     /locus_tag="VBC23_000293"
     CDS             6126..7370
                     /locus_tag="VBC23_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6842271.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            7372..8655
                     /locus_tag="VBC23_000294"
     CDS             7372..8655
                     /locus_tag="VBC23_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6842272.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            8778..9320
                     /gene="pgsA"
                     /locus_tag="VBC23_000295"
     CDS             8778..9320
                     /gene="pgsA"
                     /locus_tag="VBC23_000295"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6842273.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            9320..10159
                     /locus_tag="VBC23_000296"
     CDS             9320..10159
                     /locus_tag="VBC23_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6842274.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            10135..10977
                     /locus_tag="VBC23_000297"
     CDS             10135..10977
                     /locus_tag="VBC23_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6842275.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            10970..11764
                     /locus_tag="VBC23_000298"
     CDS             10970..11764
                     /locus_tag="VBC23_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6842276.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            11980..12519
                     /locus_tag="VBC23_000299"
                     /pseudo
     CDS             11980..12519
                     /locus_tag="VBC23_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            12625..13329
                     /locus_tag="VBC23_000300"
     CDS             12625..13329
                     /locus_tag="VBC23_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6842277.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(13464..14336)
                     /gene="sdaAA"
                     /locus_tag="VBC23_000301"
     CDS             complement(13464..14336)
                     /gene="sdaAA"
                     /locus_tag="VBC23_000301"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6842278.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(14351..15019)
                     /gene="sdaAB"
                     /locus_tag="VBC23_000302"
     CDS             complement(14351..15019)
                     /gene="sdaAB"
                     /locus_tag="VBC23_000302"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6842279.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            15265..16386
                     /gene="mnmA"
                     /locus_tag="VBC23_000303"
     CDS             15265..16386
                     /gene="mnmA"
                     /locus_tag="VBC23_000303"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6842280.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            16418..17029
                     /locus_tag="VBC23_000304"
     CDS             16418..17029
                     /locus_tag="VBC23_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6842281.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            17199..19100
                     /gene="mnmG"
                     /locus_tag="VBC23_000305"
     CDS             17199..19100
                     /gene="mnmG"
                     /locus_tag="VBC23_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6842282.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            19167..21149
                     /locus_tag="VBC23_000306"
     CDS             19167..21149
                     /locus_tag="VBC23_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6842283.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            21155..21607
                     /gene="rplI"
                     /locus_tag="VBC23_000307"
     CDS             21155..21607
                     /gene="rplI"
                     /locus_tag="VBC23_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6842284.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            21650..23005
                     /gene="dnaB"
                     /locus_tag="VBC23_000308"
     CDS             21650..23005
                     /gene="dnaB"
                     /locus_tag="VBC23_000308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6842285.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            23017..23304
                     /locus_tag="VBC23_000309"
     CDS             23017..23304
                     /locus_tag="VBC23_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6842286.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            23634..24245
                     /gene="rpsD"
                     /locus_tag="VBC23_000310"
     CDS             23634..24245
                     /gene="rpsD"
                     /locus_tag="VBC23_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6842287.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(24353..25435)
                     /locus_tag="VBC23_000311"
     CDS             complement(24353..25435)
                     /locus_tag="VBC23_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6842288.1"
                     /translation="MERFIIVKINEVKKKDGTVIYRASVYLGTDKVTGKKVTTKITGR
                     TKKEVREKAKQGAVEFIKNGSTRFKATSVTSYQELATLWWDSYKHTVKYNTQLADKTN
                     KGERKAYLHYDRIHALNKRILQYGVIMQAIPFNPAREVILPRNTKKANTKRVKHFEND
                     ELRTFFNYLNNLDKSKYRNFYEVTLYKFLLATGCRINEALALNWSDIDLDNAVVHITK
                     TLNYKQEINSPKSKSSYRDIDIDSQTITMLKQYRRRQIQEAWKLGRSETVVFSDFIHK
                     YPNNRTLQTRLRTHFKRANVPNIGFHGFRHTHASLLLNTGIPYKELQYRLGHSTLSMT
                     MDIYSHLSKENAKKAVSFFETAINSI"
     gene            complement(26451..27608)
                     /locus_tag="VBC23_000312"
     CDS             complement(26451..27608)
                     /locus_tag="VBC23_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HEO6842289.1"
                     /translation="MKKRIIVVLVALLAIGGGSAYYFLSYAPHQEAVTNFNKAVQTVK
                     SKNKTLKSEIAKAEKLVKSNQQPLDVRTLDNLKAAVATAKSDLREIPKVADKTEAIKK
                     QTKSLNQPIDYSKATQSLADSSLAYTNSVKQLAQITNPSQSFVEERLKEIDTVTGVQS
                     VTESNDPNGKLNKQGGYTASIYFTDNQVTEAVEGKDVLDKGTDAGGNIEVFKTKEEAE
                     KRNIYLSAFDSAGMFNPGSHYVFGTVVIRTSHYLTASQQKSLTQKIYEKLIEIKSKNS
                     GSTKDSKKKEVSKDNTKTSTQEQPAPSSTTDKSQESQSSSETSNSGGRGGTRGVEDSS
                     DTSSSSEMTEEELNSAIKEIQDSRDYYAPNEFENQVGDTTSSDNGDSTYHP"
     gene            complement(27863..28450)
                     /locus_tag="VBC23_000313"
     CDS             complement(27863..28450)
                     /locus_tag="VBC23_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842290.1"
                     /translation="MNRLKELRANQKKTQQDMSEFLNMSRRGYQKIENGESQIKPDKA
                     QALADYFNVSVGYLLGYSDDKKDPMLTSFELARKDDTGEYFDLQDMVTLMDISMLKNV
                     DTRDKILSNLKEYYDYYGIRMKEEHEIKDSEKFNAFLAEFQEDTNDYIIMLLTGIVKL
                     RDDIRFTLLDILAMEGEKLETVKNVVKFISDNGDN"
     gene            28605..28790
                     /locus_tag="VBC23_000314"
     CDS             28605..28790
                     /locus_tag="VBC23_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842291.1"
                     /translation="MLITSTQAKAIRRKQADKQLTSKSASAEIGVTEVTYRKVRNGGE
                     VKNGIYQKVMEWLAEDY"
     gene            28806..29408
                     /locus_tag="VBC23_000315"
     CDS             28806..29408
                     /locus_tag="VBC23_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO6842292.1"
                     /translation="MRTETWNGYDIRFVEHQGEWWAVLADICHALDLKPKRVKERLVD
                     EVVSTDHVADSLGRQQEMLIVNEFGIYDTIFSSRKPEAKSFKFWVFETIKQLRQATGL
                     EGFEVFRMLDKEHQKEAMARLTNSLDRVSKKDLIKANTITNKAVSNKFGYSKMVKKSE
                     MTPDMLVAREMILDDTVELMGIKEKFGLNISVSESIYNKN"
     gene            29644..30282
                     /locus_tag="VBC23_000316"
     CDS             29644..30282
                     /locus_tag="VBC23_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO6842293.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQGFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSADELTRYRKLEQKVIVLLELNAEYNDIKKLVL"
     gene            30381..30554
                     /locus_tag="VBC23_000317"
     CDS             30381..30554
                     /locus_tag="VBC23_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO6842294.1"
                     /translation="MTDKRAVGRPPKGEQPNNIKITVRISQGLDEKVQAYADNHGLTK
                     PEAIRKALEDLSD"
     gene            30643..31149
                     /locus_tag="VBC23_000318"
     CDS             30643..31149
                     /locus_tag="VBC23_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842295.1"
                     /translation="MRTLFQSTQKTERECAFCVLKNQNNMIKKKRNPRVLFKNVAYKL
                     SEIEGKTLTEIASFLGFGNSEVCRSTLYNWKRRKWLTFDLKNGHYRNVEVLHEVTLEK
                     MANKELKEQGLIYKASIYYEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELF
                     AELYTNMN"
     gene            31226..31558
                     /locus_tag="VBC23_000319"
     CDS             31226..31558
                     /locus_tag="VBC23_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842296.1"
                     /translation="MKYRVETNPFSKDRYTPEQREMLKKRQLSKDKAEAYFARLYNHH
                     IARVIIANVMAEYTTTFRKSATTFEEAWGALGYKQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            31555..31776
                     /locus_tag="VBC23_000320"
     CDS             31555..31776
                     /locus_tag="VBC23_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842297.1"
                     /translation="MRCIEELNEIANDILVDYEGLCGQLMDILNVLDLAAQEYSKETT
                     SAIINTSCRALKCLISDHDKMTHKYRKEL"
     gene            31779..31970
                     /locus_tag="VBC23_000321"
     CDS             31779..31970
                     /locus_tag="VBC23_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842298.1"
                     /translation="MQELNFTLTQTLILFIVLGLIGLLLSRSKPLIEIDLPEDTQAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            31981..32309
                     /locus_tag="VBC23_000322"
                     /pseudo
     CDS             31981..32309
                     /locus_tag="VBC23_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32311..32583
                     /locus_tag="VBC23_000323"
     CDS             32311..32583
                     /locus_tag="VBC23_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842299.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNRFLEVYQQPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLKRYQPPLEDTRKHYYKVSDILIFLGVDV"
     gene            32584..33442
                     /locus_tag="VBC23_000324"
                     /pseudo
     CDS             32584..33442
                     /locus_tag="VBC23_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
     gene            33454..34848
                     /locus_tag="VBC23_000325"
     CDS             33454..34848
                     /locus_tag="VBC23_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEO6842300.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIFTGIKYNEFEKTIEITKTLPWSKEKGLWTNEQTSLCIAFIDDKYRFTPRKEHI
                     EVAITALAKKNSYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     IARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVEKHPMEVEEDYFLQILAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYVLKQLGEPLDNAQSYSDY
                     PSAQTNCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDHLDNDDGWKKGENPARKR
                     LFKGGTPVSYYERV"
     gene            35141..35398
                     /locus_tag="VBC23_000326"
     CDS             35141..35398
                     /locus_tag="VBC23_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842301.1"
                     /translation="MNPNYTNHGIKKMHYHYDDLPNDQLYRLELPNGQCGYDFISKIN
                     FNTKSKPGDCDFTRPELIRRGYRWVFDCGFAIPVEKGEQTQ"
     gene            35395..35568
                     /locus_tag="VBC23_000327"
     CDS             35395..35568
                     /locus_tag="VBC23_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEO6842302.1"
                     /translation="MKIKLFYQKHKESLDDFEYRINQFTLSVSVIDIKFSEATYGNYE
                     DMSNTTSLLVLYR"
     gene            35726..36283
                     /locus_tag="VBC23_000328"
     CDS             35726..36283
                     /locus_tag="VBC23_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842303.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGSYEQPVID
                     ATYHNDDQGGHANQLVLEYAEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDDVAQET
                     ELLKEVESTDKKRVVPLFDNSQEKADKQDIFMAFISDHNQLAEHVSMNYKKMTQEDLG
                     AVLESISQGFNYLYDMAIEGELLVK"
     gene            36356..36844
                     /locus_tag="VBC23_000329"
     CDS             36356..36844
                     /locus_tag="VBC23_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842304.1"
                     /translation="MLELAIESIIKPMKKQEKTRVTGTINDQSITIDLDNLIVHYEGQ
                     EFLLETIPGTYGGKRYFFLCPDCERRCRKLFKASHAFACGTCQKLHQATLNRSKTDCQ
                     YYWQLAFKECLKVDSKAKHKHGYYSYDDFPKRPKYMRMTKYLYHWKRFHYYMDKGDKY
                     WL"
     gene            36851..37003
                     /locus_tag="VBC23_000330"
                     /pseudo
     CDS             36851..37003
                     /locus_tag="VBC23_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
     gene            36978..37355
                     /locus_tag="VBC23_000331"
     CDS             36978..37355
                     /locus_tag="VBC23_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6842305.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLSYNERYQFDIKYHPTRREH
                     LEISRECALEELDAIKYAINQLSKIDYRKILIECYLISEKKTQQDIIAELNKSQSWYY
                     ETKKRALLEFAQSYRNGVLITTA"
     gene            37424..37711
                     /locus_tag="VBC23_000332"
     CDS             37424..37711
                     /locus_tag="VBC23_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6842306.1"
                     /translation="MHALEKNTQVNFKTNSDLLEKAKAIIAAQNLDMTASFNLFLENI
                     VQNKALPFETDTDKERAELLAGLRAEIAKSFDDLEHGRVYNASEVRANLGI"
     gene            37701..38048
                     /locus_tag="VBC23_000333"
     CDS             37701..38048
                     /locus_tag="VBC23_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO6842307.1"
                     /translation="MVSDNKTHSLIIPETVQEQLQEIKSYIETTYFSEQAGANTVNNI
                     LYGLERLEFFPEAGFNADDRVGETIYPPHNTRCIVLGDYLAFYHILEDRKAVFVSDII
                     HSKQDYIKLFKKK"
     gene            38532..38828
                     /locus_tag="VBC23_000334"
     CDS             38532..38828
                     /locus_tag="VBC23_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6842308.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(38914..39453)
                     /locus_tag="VBC23_000335"
     CDS             complement(38914..39453)
                     /locus_tag="VBC23_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO6842309.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            39592..41910
                     /locus_tag="VBC23_000336"
     CDS             39592..41910
                     /locus_tag="VBC23_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6842310.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            41977..42333
                     /locus_tag="VBC23_000337"
     CDS             41977..42333
                     /locus_tag="VBC23_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO6842311.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            42467..43210
                     /locus_tag="VBC23_000338"
     CDS             42467..43210
                     /locus_tag="VBC23_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6842312.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            43194..45734
                     /gene="mprF"
                     /locus_tag="VBC23_000339"
     CDS             43194..45734
                     /gene="mprF"
                     /locus_tag="VBC23_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO6842313.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(45813..47327)
                     /locus_tag="VBC23_000340"
     CDS             complement(45813..47327)
                     /locus_tag="VBC23_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6842314.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(47330..48052)
                     /locus_tag="VBC23_000341"
     CDS             complement(47330..48052)
                     /locus_tag="VBC23_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842315.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            complement(48274..49107)
                     /locus_tag="VBC23_000342"
     CDS             complement(48274..49107)
                     /locus_tag="VBC23_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6842316.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            complement(49104..50399)
                     /locus_tag="VBC23_000343"
     CDS             complement(49104..50399)
                     /locus_tag="VBC23_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6842317.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            50502..51500
                     /gene="argF"
                     /locus_tag="VBC23_000344"
     CDS             50502..51500
                     /gene="argF"
                     /locus_tag="VBC23_000344"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6842318.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            51512..52438
                     /gene="arcC"
                     /locus_tag="VBC23_000345"
     CDS             51512..52438
                     /gene="arcC"
                     /locus_tag="VBC23_000345"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6842319.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            52532..54085
                     /locus_tag="VBC23_000346"
     CDS             52532..54085
                     /locus_tag="VBC23_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6842320.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(54195..55559)
                     /locus_tag="VBC23_000347"
     CDS             complement(54195..55559)
                     /locus_tag="VBC23_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6842321.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFIFVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(55543..56214)
                     /locus_tag="VBC23_000348"
     CDS             complement(55543..56214)
                     /locus_tag="VBC23_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842322.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            complement(56299..56970)
                     /locus_tag="VBC23_000349"
     CDS             complement(56299..56970)
                     /locus_tag="VBC23_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6842323.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
BASE COUNT        18574 a         9141 c        10807 g        18489 t
ORIGIN      
        1 aacaatttga ccatattgtg agccacatta aacatcgtac agctctaaaa aatcatacct
       61 tattttttga aggtgattac cgtgaaaaac tgtttttaat ccagtcagga catgtaaaaa
      121 ttgaacaatc agacgcttct ggttctttta tttatacaga ttatgttaga caagatacag
      181 tttttcctta tggaggtctc tttttggatg atgattacca tttttcagca gtagctatta
      241 cggatattga gtatttttct ctgccaatgg ctttatacga agaatactct cttcaaaata
      301 tcaatcaaat gaagcatctt tgtcggaaat attctaagct attacgagtt catgaaatac
      361 gacttcgtaa tatggtgact tcaagtgcca gtatgagagt tatccaatcg cttgcaactc
      421 tacttttgca agttcctaca gaaaggggac atttaccttt tcctattaca acgattgaaa
      481 ttgccaatat gagcggaact actcgcgaga cagttagtca tgttttaaag gaattacgcc
      541 aaaaagatat cgttgaaatg aaaggaaaga agcttctcta taataataaa aattatttca
      601 agaaattcat tgaataagga gtttatatga ataaaaaaga aacaagacac caattaattc
      661 gttccttagt ctctgagact aaggtgcgta cacaacatga gttaagggaa ttactagaaa
      721 aaaatggcgt ttcagtgacg caagcaacat tatctcgaga tatgaaggaa cttaatttaa
      781 ttaaggttaa cgaaagcagt gataatgcta cagaaactta ttatgagatt catagtatat
      841 ctcagaagcg ttgggaagaa cggttacgtt tttacatgga agatgcccta gtcatgctgt
      901 ttccagttca aaaccaaatc gttttgaaga cactacctgg cttagctcaa tcttttggct
      961 ccatattaga ttcgatttta ttacctgaga tcttagcaac tgtctgtggt gatgatactt
     1021 gtttgattat ttgtaataat agtgaagaag ctcaaaagtg ttttgagaaa ttaagtcagt
     1081 atacgccgcc tttcttcttc tcaaaaaaat agaaaattaa gaagattttt tcattacttt
     1141 aaagagctag aagctattaa ttctatgctc tttttatatt gacaaatgat tttagcgtga
     1201 tatgatagta acaatataat tttaatggga ttttttatat ctactatcta agaaaagagg
     1261 attttttgat gtcaaattgg gatactaagt ttttgaaaaa aggttttact tttgatgatg
     1321 ttctgcttat tcctgctgaa agccatgttt taccaaatga agttgatatg aatacaaagt
     1381 tggctgataa tttgacatta aacattccaa tcattacagc tgcaatggat acagttacag
     1441 atagtaagat ggctattgct attgcacgtg ctggtgggct tggtatcatt cataaaaata
     1501 tgtcaatagt tgatcaagca gaagaagttc gtaaagttaa acgctcagaa aatggtgtta
     1561 ttattgaccc attcttcttg acaccagata atactgtctc tgaagctgag gaattgatgc
     1621 aaaactatcg cattagtgga gttcctattg tagagacact cgagaatcgt aaattagttg
     1681 gaattattac taaccgtgat atgcgtttca tttctgatta taaacaacta atctcagagc
     1741 acatgacaag tcaaaatctt gtaacagctc ctattggtac agatcttgaa actgcggagc
     1801 gtattcttca tgagcatcgt attgaaaagc tacctttagt agatgatgag ggacgtcttt
     1861 caggacttat tactattaag gatatcgaaa aagttattga atttcctaag gcagcaaaag
     1921 atgaatttgg acgtctctta gttgcaggtg cagtaggtgt aacctctgat acttttgaac
     1981 gtgcagaagc tttgtttgaa gcaggtgcag atgctattgt cattgataca gcacatggac
     2041 attcagctgg tgtgctccgt aaaattgctg aaattcgtgc tcacttccct aatcgcacat
     2101 tgattgcggg aaatattgct acagcagaag gagcgcgtgc actttatgat gcaggtgtgg
     2161 atgtcgttaa agttggtatt ggtccaggtt caatttgtac gactcgtgtc gttgctgggg
     2221 taggtgttcc tcagattaca gctatttacg atgcagcggc tgttgcgcgt gagtacggta
     2281 agactatcat tgctgatggt ggtattaaat attcaggtga tattgtaaaa gcacttgcag
     2341 caggtggaaa tgcagttatg ctaggttcaa tgtttgcagg aacagatgaa gcgccaggag
     2401 aaacagaaat tttccaaggg cgtaagttca agacataccg tggtatgggc tcaattgcag
     2461 caatgaaaaa aggttcaagc gatcgttatt tccaaggttc tgtaaacgag gctaacaaat
     2521 tggttccaga aggtattgaa ggtcgtgtag catacaaagg ttcagtagcc gatattgttt
     2581 tccaaatgct tggtggcatt cgttctggta tgggatatgt aggtgcagcg aacataaaag
     2641 aacttcatga taatgctcaa tttgttgaaa tgtcaggagc tggtttgaaa gaaagccatc
     2701 cccatgatgt tcaaattaca aatgaggcac caaattattc agtacactaa aataaggaca
     2761 aaaaagcatc ccctatgagg atgctttttg ttgcataaaa gaaacaaata tagtaaagtt
     2821 tttgtatgaa aaattatgga aaaattttaa aagagttgag agaagacaaa ggtatatccc
     2881 taagttctct agccaaaagt gctcaattat caaaatcaac tctatcacgt tttgaaaatg
     2941 gtgagacaca aataggtatt gataaattta ttaaagcttt acaaacgttg gaggttgggg
     3001 taactatcaa tgaagtatcc attctagatt caaaagttaa ggctggaact tctaatactg
     3061 atttagagca attaacttta ctggaatctt atcgagataa tgaagatatc atgagaattt
     3121 tcagtttcca agaacaacaa tcctgtgata gaatagaaag caatgttctc aaaatattgg
     3181 ctaaactttt tatttcaaat ttagggttaa atatgaggct gcctcaagat gagattaacc
     3241 tggtagttac ttatctgaat ggtgtgacac agtacaatga tttttatttc aaagtcatct
     3301 gctattttca agatatttta cctgaagatg ttattttaaa taagattagt aatatgacta
     3361 aagaacagtt accttattct aaatcactgg tcaatttatt gataaaacaa gtcattatag
     3421 ctttggaaaa agatagtgtt gataaagcta tagtatttgc tgatcttgct aataaagtta
     3481 gtcaatagat tctctaggat tgaaaatagg taatacttaa tattccattg taaggagaaa
     3541 gaattggaag gtttacttat tgcattgatt cccatgtttg cgtggggaag tattggattt
     3601 gttagtaata aaattggagg gcgtccaaat caacaaacat ttggaatgac tttaggagca
     3661 ttgctatttg cgattatcgt atggttattt aaacagccag agatgactgc ctcattgtgg
     3721 atttttggta tcttaggtgg tatcctatgg tcagtcggcc aaaatggtca atttcaagca
     3781 atgaaatata tgggagtctc tgttgctaat ccactgtcaa gtggtgcaca attagtaggt
     3841 ggaagcctag ttggtgcttt agtctttcat gaatggacta agccaatcca atttatgtta
     3901 ggattgacag cgttgacatt attagttatc ggcttctatt tctcaagtaa acgtgatgtt
     3961 tcagaacaag ctttggcaac acatcaagag ttttcaaaag gatttgctac aattgcttat
     4021 tcaactgtag gttacatctc gtacgcagtt ttatttaaca acattatgaa gttcgacgct
     4081 atggccgtca ttttacccat ggctgttgga atgtgtctag gtgcaatttg tttcatgaag
     4141 tttcgtgtta actttgaggc tgttgttgtt aaaaatatga ttacaggtct catgtggggc
     4201 gttggtaatg tcttcatgtt attggcagca gctaaagcag ggctagcaat tgcttttagt
     4261 ttttctcaac ttggagtaat tatctctatt attggtggta ttttattttt aggtgagaca
     4321 aaaacgaaga aagagcagaa atgggttgtc atgggtatcc tttgttttgt tatgggtgct
     4381 atattacttg gtattgttaa atcttattaa aaagcaacag attaatctgt tgcttttttt
     4441 tcattaatac taatagtacc atcactaacg ttaaagattt tgagttgatc aggtaaagca
     4501 cttaaatgtt ctaaacttgt tgtagtaata aatgtttgaa cattctcttt gattccctct
     4561 aagagtttta gttgacgatg gttgtcaagt tcactcatga cgtcgtcaag taagagaatc
     4621 ggataatcgt tggtaacggt ttttattagc tcaatttctg ctaatttcaa tgataaaata
     4681 aggcttcttt gttgcccttg gctaccaaaa gtagcattga tatcattaat aaagaaagta
     4741 atatcatcac gatgaggtcc gatgcttgtg tttttcttaa agatgtctct tgagtggctt
     4801 ttagataatt ggtttaaaaa atgttcccta atacttgact tatctgtaaa ctctattgac
     4861 gatttgtagt gaatagataa atgttctaac tcagtactga taatgtagtg atgtttgtcc
     4921 gcttcatcat ttagggcttg gataaaatca aaacggtgtt caatgacacg acttccataa
     4981 tcagctaatt gttcatcaag gactgttaaa aaagttttgt cgacgttatt agtagttttt
     5041 agataagtgt ttctttgttt taatacatga ttgtaatttg aaagttcagc taagtacgtt
     5101 gacttaattt gtccaatatc tatatctaaa aattttcgtc ttagactagg tgcaccttta
     5161 actaattgaa gatcttcagg ggcaaaaagt acgacagtca tagccccaat ataatcagat
     5221 aattttgcct gtttaagatg gttaacttta gtgattcttc ctttttcaga taattgaata
     5281 tctaagttaa gatggccact tttgcggatt acctcacctg taatttgtac atcatggtgt
     5341 ttaaaatgta caagttcttt atcagaacgg gtacggtgac ttcgagtcaa agccaagaag
     5401 tagattgctt ctaaaaaatt tgtctttcct tgagcattgc ggccaatgaa aatattgagg
     5461 ttgggtgaaa aatctacttg tgcttcttca taattacgat aatgttttaa agaaatattt
     5521 tttatccaca tttttatgtc cctggaaagc gaactggttt tttgctagtt tttgatttat
     5581 ctttgttggt gtttttattc atttctttaa cgattgctga aacacgctgt ttttcagcta
     5641 tttctacatt acgttcttca atttcttgat ctgatggctt ccttataatg atctcgatat
     5701 tttgatcagg aatagtaata atatccccca ggcggagttt ctttccacgc ctattttcaa
     5761 ggtcaccgtt aaacaatact tgattatccg ctaaaaattt tttaatggct ccgccacttt
     5821 gaatgatacc aatctctttt agaagtgatt gtaaagtaat atattcatca aataatttat
     5881 aatccatgac tttttaatct ccaattttag atatcacatc attatagcat aaaattattt
     5941 tttgagttga acttatgata tggagacaag gtataaaaaa tgggagtaaa tattggttga
     6001 cgactttgtc aattttgaag aagattgaaa tttagaatta atcatatctt ggatgtttaa
     6061 tcttttttaa cgcctttaaa aatgatataa tcttagctaa caatacagaa cacaaaggat
     6121 attgaatgaa aatagttgaa ggcgtttctc tacatttaat taaaaaccaa caatttaaga
     6181 ctaatcatct tacttttcga ttttcaggtg attttaataa taaaacagta gcacgtagat
     6241 cgctcgttgc tcaaatgtta gttactgcaa atgctaagta ccctaaggta caagaatttc
     6301 gagaaaagtt agcttcctta tatggtgcta gtttatcaac taagatttct actaagggtc
     6361 ttgttcacat tgttgatatt gatattgtat ttgttaaaaa tacctttact ttagaacaag
     6421 aaaatatcgt tgaacaaatt attacattct tggaggatat gctgttttct cctttaattt
     6481 ctttagaaca gtatcaaaca tctatttttg atactgagaa gaaaaacctt attcaatatt
     6541 tagaggccga tattgaagat aatttttata gcagtgactt agcactaaaa tctttattct
     6601 acaataataa gacacttcgt ttacctaagt atggtacagc atctttagtg gaatcagaaa
     6661 attcatttac agcttatcaa gaatttcaga agatgcttaa ggaagatcaa ttagatattt
     6721 ttgtcgtagg agattttgat gactatcgaa tgattcaggc atttaatcgt atggcatttg
     6781 agccacgtca caaagtatta gcttttgatt atacgcagac ttatgaaaat atcacgagaa
     6841 gtcaagtaga agataaggac gttaatcaat ctattatgca attggcttat catcttccaa
     6901 taacttataa agatgaagat tattttgctt taatagtatt taacggttta tttggtgctt
     6961 ttgcccattc cttgttgttt acagaaattc gagaaaagca aggtttagcc tatactatag
     7021 gtagtcaatt tgacagcttt acaggtctct ttacgatata tgcaggtatt gataaagaga
     7081 atcgtgagcg ttttttgaag cttattaata agcaattcaa taatattaag atgggcaggt
     7141 tctcttctac attattgaaa caaacaaaag atatattgaa aatgaactat gttttagctt
     7201 ctgataatcc taaggtcata gttgaccaca tttatcatga acattattta gatcagtttc
     7261 atacttcagc actattcatt gacaaagtgg acgatgtaac aaaatcagat attgtcagcg
     7321 tagcaacaaa attaaaatta caagcttttt attttttgga aggaaactag gatgacagtt
     7381 aataaaataa cttatcagaa ccttcaggag gaagtatata agttaacatt agagagcggg
     7441 ctaaatgtat atcttattcc aaaaccatca tttaaggaaa ctgttggagt attgacagca
     7501 aattttggat cactccatac aagatatacg aggaatggtt gtgtagaaca ttatccggca
     7561 ggaattgctc acttcttaga acataaatta tttgaactgg ataaaggaca agacgctgca
     7621 actcaattta ctaaatatgg cgccgagagt aatgccttta caacttttga taaaactagt
     7681 ttttattttt caactataag tcacattaca aactgcttag atatactact tgactttgta
     7741 ttgacaacaa actttacaga agagtcaatt actaaagaaa aagacattat caaacaagaa
     7801 attgaaatgt atcaagatga cccagaatat aggctttatc aaggagtgtt atcgaacctt
     7861 taccccaact ctccattagc atttgatatc gctggagatt accaatcaat ctctcaaata
     7921 accttaaccg atttacaaga aaatcacaaa gacttttatc agttgtctaa tatgaattta
     7981 gttcttgttg gacaatttag tccacaagaa attataactt accttcaaaa aaattctcat
     8041 ttaacaaatt actcacagaa tattgaccga gattctatta gtcttgaacc tgttataaaa
     8101 aacaactctt gccatatgac agtgacaaaa cctaaattag ctattggtta tcgaaaatca
     8161 agtcatatga gacatggttc ttacctaaaa gagaaaatcg gattacagct attttttgca
     8221 atgcttttag gatggacttc tacaattaat caagactggt acgaatctgg tcaaattgat
     8281 gactcttttg atatagaaat tgaagttcat ccagattttg agtgcgttat tatttcttta
     8341 gatacgacag agccaattgc tttttcaact cagttgaggt tattgctaaa aaatgctttg
     8401 caatcatctg atcttaccga aagccactta caaaacgtta agagagagct ctatggcgat
     8461 tttttaagga gcttagattc tattgaaaat ctcgcaatgc aattcgtcac ctatttatat
     8521 gatggaaaaa cgatgtattt agatttgcca tctatcgttg aagaattaga cttggaagat
     8581 gtcattacga ttggtaagga tttcttagat aatgctgaca catctgactt cgttattttt
     8641 ccaaaatctt cgtaaataat ctgaaatttt gatataataa ggaagcttag acatctataa
     8701 agttggtatg tcaaaagtct tttactagtt ttgaaataac ataatatagg aatactatgt
     8761 ttccaaaagg agaatttatg gtgaagaaag aaaatattcc gaatctatta actgtagtca
     8821 gaattttaat gattccgtta tttatagtat taacttcagt aacaacaagt acaacttggc
     8881 atattgttgc agctattgta tttgctattg caagcttgac cgattaccta gacggttatc
     8941 tagcacgtaa atggcaagta gtaacaaatt ttgggaaatt tgcagatcca ttagctgata
     9001 aaatgctagt tatgagtgct tttatcatgt tagttggact agacttagca ccggcttggg
     9061 tttcagctat tatcatttgt agagagctag cagttacagg tttgcgttta cttttagttg
     9121 aaacaggtgg aacagtactt gctgcagcta tgccagggaa aataaagacg gctactcaaa
     9181 tgtttgcagt tatttttttg ttagtacatt ggatgacact tggtaacatc atgttataca
     9241 ttgctttatt ctttaccttg tattcaggct acgactattt taaaggtgca ggcttcttat
     9301 ttaaggatac ttttaaataa tgacaaatat tattactgtt aataatctct tttttaaata
     9361 tgatagtaat caaacacatt atcaattgga gaatgtatcg tttcatgtga aacaaggaga
     9421 atggctatct attatcggtc ataatggttc tgggaaatcc actactgttc gtttaataga
     9481 tgggctttta gaggcggagt caggccagat tatcattgat ggacaagagt taaccgaaga
     9541 taatgtctgg gagttacgtc acaagattgg catggtattt caaaatcctg ataaccaatt
     9601 tgtaggggca actgttgaag atgacgtcgc ctttggtttg gaaaataaag gtattccttt
     9661 gaaagacatg aaagaaagag tggaccaagc acttgatttg gtaggcatgt ctgaatttaa
     9721 aatgagggag cctgcacgtt tatctggggg acaaaagcag cgtgtagcca ttgcaggagc
     9781 tgtagctatg cgtccacaag ttatcatctt ggatgaggcg actagtatgc ttgatcctga
     9841 gggacgattg gaactgataa gaacgatacg agctattcgt caaaaatata atcttactgt
     9901 tatttcaatt acacacgact tagatgaagt tgcattgagt gatcgtgtca tcgttatgaa
     9961 aaatggtaaa gttgagtcga catccactcc aaaagcgtta tttggtcgtg gaaatcgttt
    10021 gattagttta ggtttagatg ttccctttac aagtaggtta atggcagaac tggctgctaa
    10081 tggtcttgat atagggacag agtatcttac agagaaggaa ttagaagaac aattatggga
    10141 attgaattta aaaatgtaag ttatacctat caagccggca ctccttttga agggcgtgcc
    10201 ctttttgacg tcaatctgaa aattgaagat gtttcctata ccgcgttcat tgggcacaca
    10261 ggttctggaa aatcaactat tatgcaactt ttgaatggtt tacatattcc tacaaaaggt
    10321 gaggtaattg tcgatgattt ttctattaaa gcaggggaca agaacaaaga aatcaaattt
    10381 ataaggcaaa aagttggttt agtttttcaa tttccagaaa gtcagctttt tgaagagaca
    10441 gttttaaagg atgttgcttt tggaccacaa aattttggta tttctcagat tgaagctgaa
    10501 aggctggctg aagaaaaatt aaggttagtt ggtatcagtg aggatttatt cgataaaaat
    10561 ccatttgaac tttctggagg gcagatgagg cgggttgcta tagctggtat tttagcgatg
    10621 gaacccaaag tactagtact ggatgagcca acagctggac ttgatcctaa gggaagaaaa
    10681 gaattaatga ctctttttaa aaatcttcat aaaaaaggaa tgactatcgt cttagtgact
    10741 cacttaatgg acgatgtagc ggattatgct gactatgtgt atgttttaga agcagggaaa
    10801 gtaaccttat caggacaacc aaaacagatt tttcaagaag tagaactttt agaaagtaaa
    10861 caattaggag ttcccaaaat caccaagttt gctcaaagac tatctcataa gggattaaat
    10921 ttacctagtt taccaattac tattaacgaa tttgtggagg ctattaagca tggataaatt
    10981 gattttggga cgttacatac caggtaattc tctcattcat aaattagatc ctcgaagtaa
    11041 gttactagcc atgttgcttt ttatcattat tgttttttgg gcaaataatg tagtgacaaa
    11101 tgtgatcgta ttcattttta cgttggttat tgtgggttta tctcagataa aattttccta
    11161 ttttttcaat ggtattaaac cgatggttgg tattatttta tttacaaccc tgttccagat
    11221 gctgtttgca caaggcgggc aggttatctt ctcgttttgg atttttagca tcactagcct
    11281 tgggttacaa caagcagcac ttatttttat gagatttgtt ttaattatct tcttttcgac
    11341 attacttacc ttaactacta ctccgttaag tttagcagat gctgttgaat ctttgttaaa
    11401 accattggaa gtactgagag taccagcaca cgagattggt ttgatgttat cccttagctt
    11461 acgtttcgtt cctactttaa tggatgatac aacacgtatc atgaatgctc aaagagctcg
    11521 tggagttgat tttggagagg gaaacctaat tcataaggtt aagtccatta tccctatttt
    11581 gattccatta tttgcttcta gttttaaaag agcagatgcg ttggcaattg ctatggaagc
    11641 aagaggttat caaggtggag ccaatcgtag taagtaccgt ttattgaagt ggactgttcg
    11701 ggatactttt agtatattac tgatgttatt attgggtttg agtttgtttc tattaaaaaa
    11761 ttaaaaggtt aaggtcagtt tatggtctta gccttttcat ttatataatg tgaaaagctc
    11821 aacaacttta cttaaagaaa actaaaaatt gttttttgga ggccttttct ttattaattt
    11881 acaatttatt tgtaataaat ttcatctaag gcaaaacaat tagtaatatt tacaaggtat
    11941 gatagtacgc gtatcaatta atgaaagaga gcatatatta tgtcaataac ctcggttaaa
    12001 aaatcaaaac catttaaatt aggagtggca ggtcttttag tgggtgcttc attagcttta
    12061 ccactttcag taagcgcagc atcttatacc gtgaaatcag gtgatacctt atcagctatt
    12121 gctaaaaatc ataaaactac ggtacaatag ttagtgtctc tcaatagtat cagtaacgct
    12181 gatgtcatca gtataggtga tgttttaaaa ttggataatt ctacagctag tcaagcagaa
    12241 gcaaaatctc aaccaacaat tgaaaattca atgaattctt catcaaattt gagttcaagt
    12301 gattcagctg caaaagaaga aatagctcgt cgtgaatcaa atggtagtta tactgcacag
    12361 aatggacaat attatggaag atatcaactg tctcaatctt acctaaatgg cgacttatct
    12421 cctgaaaatc aagaaaaagt agcggacaat tatgtggctt ctcgttacgg atcttggtcg
    12481 gcagcgctat cattttggaa tagtaacggt tggtattaat aaaagttgat aaaacaaaga
    12541 gatttactta aaattaaatc tctttgtaat atttttaaca tctattcaat tgcaaggtaa
    12601 caaataaagt ataatatagg taacatgaat aaaagaagaa aattatcaaa attgaatgta
    12661 aaaaaacaac atttagctta tggagctatc actttagtag cccttttttc atgtattttg
    12721 gctgtaacgg tcatctttaa aagttcacaa gttactactg aatctttgtc aaaagcagat
    12781 aaagttcgcg tagccaaaaa atcaaaaatg actaaggcga catctaaatc aaaagtagaa
    12841 gatgtaaaac aggctccaaa accttctcag gcatctaatg aagccccaaa atcaagttct
    12901 caatctacag aagctaattc tcagcaacaa gttactgcga gtgaagaggc ggctgtagaa
    12961 caagcagttg taacagaaaa tacccctgct accagtcagg cacaacaaac ttatgctgtt
    13021 actgagacaa cttacaaacc tgctcaacac cagacaagtg gccaagtatt gagcaatgga
    13081 aatactgcag gggcggtcgg atctgctgct gcagcacaaa tggctgctgc aacaggagtc
    13141 cctcagtcta cttgggaaca tattattgcc cgtgaatcaa atggtaatcc taatgttgct
    13201 aatgcctcag gagcttcagg acttttccaa acgatgccag gttggggttc aacagctaca
    13261 gttcaggatc aagttaattc agctattaaa gcttatcgtg ctcaaggttt atcagcttgg
    13321 ggttactagt gaatagctat aaattaactt tacactattg tcaataataa ttacagattg
    13381 ctaaaacaaa taaataaaaa cgctacagta aagctgtagc gtttttattt attttcaggg
    13441 aaagagtaga aaattgataa actttattcc ccaaaaattt ctttgctata acgtcttcct
    13501 gtcggcgtgg cagcaagtcc tccttctgca gtttcacgaa aagcagtcgg taaacttgat
    13561 ccaacttgat acattgcatc aataacttca tctactggaa tttgcgattc aataccagcc
    13621 aaggccatat cagcagcaac aagtgcaaaa cttgatccaa gagcattccg cttcacacaa
    13681 gggacttcaa ctaaacctgc aacagggtca cagataagtc caagcatatt tttaataaca
    13741 aatgctatag cttggctagc ttggaaagga gtacctccag cagccataac taaagcagcc
    13801 gcagccatag cactagctga cccaacttca gcttggcaac ctccttctgc acctgagata
    13861 gaggcattat taccaatgac aagaccaaat gcgccggctg taaatagaaa attaagttgc
    13921 tcttcttctg ttaaattaag cttttcaatg gctgtagaaa tcacagctgg taaacatcct
    13981 gcactacctg cagttggtgt tgcacagacc agtcccatct tagcatttaa ctcattaaca
    14041 gccatagcat tcctaacggc agctaggatt gtggtatctg aaatagtttt tcctgattgt
    14101 aaatattgat ccatcttgac agcatcaccg cctgttaaac cactgattga tttactaggg
    14161 gtcaatccat caataacaga agctttcata acctcaaggt ttcgggacat aatataacga
    14221 atttcttcac gacttctacc agtcatttca atttccgttt ggatcatgag ctctgctatg
    14281 ttacccttat gttggctatt agcttgctct accagctctt caattgtata aaacatagtt
    14341 accctttctc ttaatcaaag aagttgacat tgtgtaaatg aggaatagct tctatctttt
    14401 tgacagcttg ttgacaatct cgtgaatcta cttctataat catgatagct ttttctcctg
    14461 cactttcgcg tgttacattc atttgagcaa tattaatgtt aaagtcagat aaaatatcag
    14521 ttactttagc aatcatgcca ggtatatctt gatgaacgat aattaaagta ggagtattca
    14581 ttgttaatga aactgagaat ccattcaact cagtaacttg gatgtttccg ccaccaattg
    14641 acactcctgt aatactcatg ctcctatccc catttttaac agttattttc gcagtatttg
    14701 gatgtggtga attactatct tttaggatat cccagtaaat cttaattcct ttttgatgag
    14761 caatttcaag agagttttta atatctggat tatctgtatc cattcctaga atccctgcaa
    14821 ccaatgcttt atcagtaccg tgtccttggt aagttttagc aaaagaattg tataaatgaa
    14881 aggttacttc actaggttca ccaaaaatag aatggacaac tttaccaatg cggacagctc
    14941 ctgcagtatg actacttgat ggtccaatca taacaggacc aataatgtcg aagaccgatt
    15001 gaaattttaa atgtttcata gtttttcctc gtgatttttg tacattctca ttataacaga
    15061 ttatttctaa aaaatatcat aaaaagataa aaaaactgca atgtaataaa tatttatcac
    15121 aacaaaaaca tacaacttga caacaaatag tgctataata tataagcact tttatagtgt
    15181 tttttagtca atatgattga tctaagaaca acttggccct tcaagcctaa gttaaaaata
    15241 aaactttttg atgggagaat tcttatgact gataattcaa acattcgtgt cgttgtaggc
    15301 atgagtggag gtgttgattc atcagtaact gccctgcttt taaaagagca aggttatgat
    15361 gttattggtg tttttatgaa aaattgggat gacacagatg aatttggtgt ttgtacggct
    15421 actgaagatt ataaagatgt tgctgctgtt gctgatcaga tcggcatacc atactactct
    15481 gtcaactttg aaaaagagta ttgggacagg gttttcgaat attttttagc agaatatcga
    15541 gcaggtcgta cgccaaatcc agatgttatg tgtaataagg aaattaaatt taaagcattt
    15601 ctagattatg cgatgacatt aggtgctgac tatgtagcaa caggtcatta tgcacaagta
    15661 acacgtgatg agaacggaat agttcatatg cttagaggag cagataataa taaggatcaa
    15721 acttattttc taagtcaatt atctcaggag caattgcaaa aaacgttatt tccacttggt
    15781 catttacaaa aaccagaagt tcgccgtata gctgaagaag caggacttgc gaccgctaag
    15841 aaaaaagact ctactggtat ttgttttatt ggagaaaaaa atttcaagga tttcctgggt
    15901 caatatttac cggctcaacc aggtcgtatg atgactgttg atggtcgtga catgggtgaa
    15961 catgctggtt taatgtatta tactattggt caaagagggg gccttggtat tggtgggcaa
    16021 catggtggag ataataaacc ttggtttgta gttggaaagg atttaagtaa aaacattctt
    16081 tatgtaggtc aaggtttcta tcatgactca ttgatgtcaa ctagcttgac agcttctgaa
    16141 atccacttta cacgtgatat gcctaatgaa tttaagctag aatgtacagc taagttcaga
    16201 tatcgtcaac ctgacagtaa agttacagtt tacgtaaaag gtaatcaagc aagggttgtg
    16261 tttgatgatt tacaacgagc aatcacacca ggtcaagctg ttgtttttta taatgaacaa
    16321 gaatgtttag gcggtgggat gattgatcag gcttatcgtg atgataaaat ctgtcaatac
    16381 atttaattga cacttaatta ttaagtgagg taatcgtatg atttcaaaat ttattttagc
    16441 tttcatggct tttttcgcca ttatgaatcc catttctaat ttgccagctt ttatggcttt
    16501 agtagctgat gatgatcaga agatttcacg tcggattgca gcaaaaggag tattacttgc
    16561 ttttgtcatt attgtaatct ttgtattaag tggccactta ctttttaact tatttgggat
    16621 taccctagcc gccctaaaaa tatctggtgg aatattggta ggcattatag gttataaaat
    16681 gattaatggt atacattcac caaccaataa aaacctagag gagcataagg atgatccaat
    16741 gaatgtggca gtttcaccat tagcaatgcc ccttttggca ggtccgggta ctattgcaac
    16801 tgctatgggg ttatcaagtg gtggtttatc tggcaaactc attactatac ttgcttttgc
    16861 catattatgt gtgataatgt atgttatttt aataagtgca aacgaaatta ctaaatttct
    16921 tggaaaaaat gcaatgacta taatcaccaa aatgatggga cttattttga tgactatcgg
    16981 gatagagatg ctaataacag gcataaaaat tggttttcat ttaacatagc ctatcccctc
    17041 tggttgactt ttaaaagata aatgctaaaa taaatttaac aataactatg atggtcaaag
    17101 ctcatggaat ttgtaagtct ttttgacgta caattttcga gagttttggc tatttttgtg
    17161 ttttaatgaa ttagactttg aaattggaaa ggaataaaat gacacataat tttgcagaaa
    17221 actatgatat tatcgttgtt ggagcgggtc atgcaggtgt ggaagctagt ctagctgcta
    17281 gtcgtatggg atgtaagaca cttctagcca ctatcaactt agaaatgtta gcatttatgc
    17341 cttgtaatcc ttctataggt ggctctgcta aaggtatcgt tgtcagagag attgacgctt
    17401 taggtggtga aatgggtaaa aacattgata aaacctatat acaaatgaaa atgcttaata
    17461 caggtaaggg gcctgctgtc cgtgccttaa gagcacaagc tgataaagcc ctctatgcac
    17521 aaacaatgaa gcaaactgta gaaaagcaag aaaatcttac cttaagacaa gctatgattg
    17581 acgaaatact tgttgaagat ggaaaggttg tgggggttcg tacagcaact aatcagaaat
    17641 tttcagcaaa atctgttgtt ataacaacag gtactgctct tcgaggagag attatattag
    17701 gtgatcttaa gtactcctcg ggccctaata atagtttagc ttctgtgaca ttggcagata
    17761 atttaagaga cctaggatta gaaattggtc gtttcaaaac tggaacacct cctcgtgtaa
    17821 aagcctcctc tattaattat gaaaagacag agatacaacc tggggatgag caaccaaatc
    17881 atttttcatt tatgtctagg gatgaagatt atattactga ccaggtaccg tgctggttga
    17941 cctatacgaa tactttgagt catgacatca tcaatcaaaa tctacaccgt gctcctatgt
    18001 tttctggaat cgttaaggga gttggtccta gatattgtcc gtcaattgaa gataaaattg
    18061 tgcgttttgc tgataaagag cgccaccaac tcttcttaga accagaaggt agatacactg
    18121 aagaagttta tgttcaaggg ctatcaacga gtcttcctga ggatgttcaa gttgatttat
    18181 tacgttctat aaaagggctt gaaaatgctg aaatgatgcg tacaggctat gctattgagt
    18241 atgatattgt attgcctcat caattaagag caacactcga aacaaaagta attgctggtt
    18301 tgtttactgc aggacagact aatggtactt caggttatga agaagctgct ggtcaagggt
    18361 tggttgctgg gatcaacgct gctcttaaag ttcaagggaa gccagaactt attttaaaac
    18421 gaagtgatgc ttacattggt gtcatgattg atgatttagt taccaaaggg actctagaac
    18481 catatcgtct gttgacaagt cgcgcagaat atcgtctgat tcttcgtcat gataatgcag
    18541 atatgcgttt aacagaaatc ggttatgaga ttggcctagt agatgaagag cgttatgcga
    18601 tatttaaaaa gcgtcaaatg caatttgaaa atgaattaga acgtttagac tctataaagt
    18661 taaagccagt ttctgagaca aataaacgga ttcaagaatt gggctttaaa cctttgacag
    18721 atgctttaac tgccaaagaa tttatgcgcc gcccacaaat cacatatgca gtagcaacgg
    18781 attttgttgg ttgtgctgat gagccattag acagtaaggt tattgaactt ttggaaactg
    18841 aaattaaata cgaaggctat attaaaaagg ctttagatca agttgcaaaa atgaaacgaa
    18901 tggaagaaaa acgtattccc cctcatattg actgggacga cattgattct atcgcaacag
    18961 aagctagaca aaaattcaaa aaaattaacc cagaaacgct aggtcaggct agtcgtattt
    19021 caggagttaa tccagccgat attagtattt taatggttta ccttgaagga agacaaaaag
    19081 gtcgtaaaaa tatcaattga taatagtgtt cgcccctgtt tatcaggggc gttttgtgtt
    19141 ataattaggt cttaagaggt tatttaatga aaagatttcg ttttgcaacc gtccatttag
    19201 ttttaatagg cctgatttta tttggactat tagctatttg tgttaggtta tttcaatcct
    19261 atacggctct attattggct atttttgttg ctttatcttt tgttgttgcc ttattatatt
    19321 accaaaagat aacttatgaa ctatctgaag ttgaacaaat tgaattactt aatgatcaaa
    19381 cagaagtttc tcttaagagt cttcttgaac aaatgccagt tggtgttata caatttgatc
    19441 ttgaaacaaa tgatattgag tggtttaacc cttatgcaga attgattttt actggggata
    19501 atggtcattt tcagtctgct actgttaaag atatcattac tagtagacgt aatggaacgg
    19561 ctgggcaatc atttgaatac ggtgataaca aatattcagc ttatttggat acagagacgg
    19621 gtgttttcta tttcttcgat aatttcatgg ggaatagaag aaactatgat agttcaatgc
    19681 tacgtccagt tattggtatt atttccattg ataactacga tgatattatg gatactatgt
    19741 tagaggctga tatgtcaaag attaacgcct tcgtgactag ttttatttct gattttacac
    19801 agtctaaaaa tattttttat cgtcgtgtca atatggatcg atactatatt tttaccgact
    19861 atagcgtttt aaatacattg ataaaagata aatttgatat tttaaatgag tttcgaaaac
    19921 gcgctcagga aaaccattta tcattaacat taagtatggg aatttcttat ggtgatggta
    19981 accataatca aattgggcaa attgctttag aaaatcttaa tacagcactc gttcgcggag
    20041 gagatcaaat agtagtacgt gaaaatgata gtagtaaaaa agcactttac tttggtggtg
    20101 gtgctgtttc aactattaaa cgttcacgaa cacgtaccag agctatgatg actgctattt
    20161 ctgaccgttt gaaagtagtt gatagtgtct ttattgtggg acacaggaaa ttggacatgg
    20221 atgcactagg tgcatctgta gggatgcaat tttttgctag taatattgtt aatgccagtt
    20281 atgtagttta tgacccaaat gacatgaatt ctgatgttga aagagcaatt gattacttgc
    20341 aagaagacgg cgaaacacgt ctcgtaagtg ttgagagagc ttttgaatta attactcaaa
    20401 attctttatt agtcatggta gaccattcta aaacagcttt aaccttatct aaggaattct
    20461 ttaacaagtt tgcagatgtt attgtggtag atcatcatag aagagatgaa gattttccaa
    20521 aaaatgctgt tctttcattt atagaaagtg gcgcaagtag tgccagcgaa ttagttacag
    20581 aattgattca atttcaacaa gctaaagata aattaagccg cagtcaagca agtattttaa
    20641 tggctggtat catgcttgat acaagaaatt ttgcatctaa tgtaacgagt agaacttttg
    20701 atgtggcaag ctatttacgc ggtttaggta gtaatagtat ggctattcaa aaaatatcag
    20761 ctacagattt tgatgaatat cgtttaatta atgaactgat tttaaaaggt gagcgtattt
    20821 atgataacat cattgttgct accggagaag aacataaagt ttactcgcat gttatagcta
    20881 gtaaagcagc tgacaccatg ttgacaatgg caggtattga ggctactttt gttattacta
    20941 aaaacagttc aaatattggt atttcagcgc gcagtagaaa taatatcaat gtccagcgta
    21001 ttatggaaaa acttggtggt ggcggacatt ttagttttgc tgcttgtcaa atacaagaca
    21061 agtctgtaaa gcaagttaga cgtatgttac tggaaataat tgacgaagat ttaagagaaa
    21121 atagtacagt cgaaaataga agggattaat tgagatgaaa gttatttttt tacaagatgt
    21181 taaggggaaa ggaaaaaaag gtgaggttaa ggaagtacct actggttatg ctcaaaactt
    21241 cttattaaaa aagaatctag ctaaagaagc aacgacacaa gctatcggtg aattaaaagg
    21301 aaaacaaaag tccgaagaaa aagcacaagc agaaatttta gcacaggcaa aggagctgaa
    21361 aacacaatta gaatccgaaa ctacccgagt gcaatttatt gaaaaggtag gtccagatgg
    21421 tcgtacgttt ggttctatca cagccaaaaa gattgctgaa gagttacaaa aacaatacgg
    21481 cattaaaatt gacaaacgtc atattgattt agatcatact attagagcga ttggtaaggt
    21541 tgaagtacca gtaaaattac ataaacaggt ttcttcacag attaagcttg atatcaaaga
    21601 agcgtaaact gagagatgat aaaggttgac aagtcaggag gaatgaagtt tggtagaagt
    21661 atctgagcta agagtacaac ctcaagacct gttggcagaa caggccgttc taggatctat
    21721 ctttatctca ccagagaaac tgattatggt gagagaattt ataagtccag atgattttta
    21781 taaatattca cacaaagtta tcttcagagc gatgattacc ttagctgata gaaatgatgc
    21841 aattgatgct gctactgtta gaaatatttt agatgaccaa ggtgatcttc aaaacattgg
    21901 tgggttgggc tatattgttg aattagttaa tagtgttcca acaagtgcta atgcggagtt
    21961 ttatgccaaa atagtttctg agaaagccat gttgcgagat atcatttcta agttgacaga
    22021 tactgtcaac atggcctatg aaggaaatga ttctgatgaa attattgcca ccgctgagaa
    22081 ggctttagtt gatattaacg aacacagtaa tcgtagcggg tttagaaaga tatctgatgt
    22141 tttaaaagtt aattacgaaa acttagaatt acgttcacag caaacctcag atgtcacagg
    22201 tttaccaact ggatttagag atttagacag gattactaca ggtttacatc cagatcaatt
    22261 aatcatctta gcggctcgtc ctgcagtagg taaaacagcc tttgttttaa atattgctca
    22321 aaatgttgga actaaacaaa atagacctgt agctattttt tcacttgaaa tgggggctga
    22381 gagtttagtt gaccgtatgt tggcagcaga gggaatggtt gattctcata gtttacgaac
    22441 tggtcagttg acggatcaag attggaataa tgtaacgatt gctcaaggag ctttggcgga
    22501 tgcgccaatt tatattgatg atacgcccgg gattaaaatt acagaaatta gagcacgctc
    22561 taggaagtta tctcaagaag ttgatgatgg tttaggttta atcgtcattg actacctcca
    22621 attaatttca ggaactagac cagaaaatcg tcagcaagaa gtttctgaaa tttcaaggca
    22681 gttgaagatt ttagctaaag aattaaaggt tcctgttatc gcattaagcc agctctctcg
    22741 tggggttgag caaaggcagg ataaacgacc ggtcttatct gatattcgtg aatctggatc
    22801 gattgaacaa gatgctgaca ttgttgcttt cttatatcga gatgattatt atcgtagaga
    22861 gggcgaagaa gcagaggaaa ttgtagaaga taatactgtt gaggttattt tagaaaagaa
    22921 ccgtgctggt gcgcgtggga cagtaaaatt aatgtttcaa aaggaatata ataaattttc
    22981 tagtatagct caatttgaag aataggagat gttaatatga gtgatgcatt tgcagatgtt
    23041 gcaaaaatga aaaaaataaa agaagatatc aagtcacatg agggacagat ggtcgaactt
    23101 actctcgaaa atggtcgtaa gagggaaaaa aataaaattg ggcgcctaat tgaagtttac
    23161 ccatctttgt ttatcgtaga gtataaggat actgcagctg taccaggagc aattgataat
    23221 acttatgtag agtcttacac ttattcagat attttaactg agaaaacttt aattcgttat
    23281 tttgatgacg agtcagcaga atagtatgaa aacctcgact gaggtttttt gtttctataa
    23341 aaaaatttac taattctcta ttggtggtta gaattaagaa tctttcttat tataattgga
    23401 aatacttatt agatgtctat ttgtatttaa ggtttaagag acataaaaac ttgattcaat
    23461 attattttca tgatataata tagcttgtgt gaaataacag cagaaaaata tgaagctcgt
    23521 caacaggtgg ttaactaatc tagtaccctt tgctgtttag gcgaaaatcc cctgcacgaa
    23581 tcaggatttt ttaaatcgtt atttcctcaa ttattttaga ggaggacatt tagatgtcac
    23641 gttatactgg tccatcatgg aaacaatcac gtcgccttgg tttatcactt acaggcacag
    23701 gtaaagaatt ggcacgtcgt aactacgtac ctggtcaaca cggtcctaat aaccgtagca
    23761 agctttcaga atatggcttg cagttagctg agaaacaaaa acttcgtttt tcatacggac
    23821 ttggtgaaaa acaattccgt aacttgttcg tacaagctac taaagcaaaa gaaggaactc
    23881 ttggtttcaa ctttatggtt cttttagagc gtcgtcttga taacgttgtt tatcgtttag
    23941 gacttgcaac aactcgtcgc caagctcgtc aatttgttaa ccatggtcat atccttgttg
    24001 atggtaaacg tgttgatatc ccatcatacc gcgttactcc aggacaagtt atttcagttc
    24061 gtgaaaaatc aatgaaagta cctgcaatcc ttgaagctgt tgaagctact ttaggacgtc
    24121 cagctttcgt atcatttgat gctgaaaaac ttgaaggttc attgactcgc cttccagaac
    24181 gcgatgaaat taacccagaa atcaatgaag cacttgtcgt tgaattctac aacaaaatgc
    24241 tttaatttta agattaaact tactgaaagc ctacaacagt gggctttttc tcttatctta
    24301 aaacattgat ttctgggttt gcttactttt ttgctaactt ttgatttttt tgctatattg
    24361 agttaattgc tgtttcaaag aatgagacag ctttttttgc attctctttt gataaatggc
    24421 tataaatatc catagtcata gatagagtag agtgccctaa cctatattgg agttctttgt
    24481 aggggatacc tgtgttgagc aataaactag cgtgagtgtg acgaaaacca tgaaagccta
    24541 tattcggtac attcgctctt ttaaaatgtg ttcttaatcg agtttgtaag gttctattgt
    24601 tggggtactt atggataaaa tcagaaaata ccactgtttc tgaacgacct aacttccatg
    24661 cttcttgaat ctgtcttcgt ctatactgct taagcattgt aattgtttga ctatctatgt
    24721 caatatcacg atagcttgac tttgactttg gactattaat ttcttgcttg taatttagcg
    24781 tttttgtgat atgaacaacg gcattatcca agtcgatatc tgaccagttt agagctaacg
    24841 cttcattaat gcgacaacct gtagctaaca aaaacttata aagtgtgact tcgtagaagt
    24901 ttctatattt acttttatct agattgttta agtagttgaa aaatgttctt agctcatcat
    24961 tttcaaaatg ctttactctt ttagtgttag ctttcttagt gttgcgaggg agaatgacct
    25021 cacgagcagg gttgaatggt atagcttgca tgataacacc atattgtagt atacgtttat
    25081 ttagcgcgtg tattctgtca taatggagat aagcttttct ttctccttta ttagttttat
    25141 cagctagctg agtattgtat tttacggtat gtttgtaact atcccaccat aaggttgcaa
    25201 gttcctgata gcttgttacg gaagtagctt tgaagcgagt agaaccattt tttataaact
    25261 caacagctcc ttgcttagct ttttctctaa cttctttctt agttcgtcct gttattttag
    25321 tggttacttt cttacctgtt actttatcag taccaagata aacactggca cgataaatta
    25381 ccgtaccatc tttctttttt acttcattta ttttcacgat tataaacctt tccatcagca
    25441 ggcaagctat tattaaaagg gttttagatt agtttatatc atggtataat aacggtatcg
    25501 ggttaatatc cgaaatatta taaatagaaa attgtgatat aatattgttg ttttttaaga
    25561 ccttgcgagc gtggaaaact tgcgaggtct ttttttgttt ggttttagta agttggtaaa
    25621 tttttagata gcccagaaaa gctggctgtc tcttggctat tgttagccgt atatatcaaa
    25681 aattgtaatt cattcatggc tattccttta aggttacccc catcattaaa aaatgttgtt
    25741 caatccgagt ttggaaaccg gtgggaaggg tcaactgtcc aaatattcgc tttttttctc
    25801 acacgacccc cctccccagg gagaaaaact agaaaggaga tgtgaatttt tggatgaatt
    25861 aaaacgttta gaatagcaat ctcatcacat ttataagcaa aattaacaca gacagtagca
    25921 aaagaacttc caaattattc aacgttgagt aatttaggta gttcagccct ctacctcatc
    25981 gctacccttc cagatgacca aaagcaagaa cagcttgaat aacctatggc ttcctatggt
    26041 ggaaggctga cgctcctgaa tcgttcaacg ttgagagatt tggcaactcc gttgacatgt
    26101 tgctgtagtc cctaaatgtt ggtacgtgcc aacataatac gtcaacctat ttcaatagtc
    26161 ctaaaacgct cggtaaattc acttaaagaa cttaaaaaaa cttgatatat cggtcgttta
    26221 ttaatttatt ttttccgttg tgatagcttc taagatcagt agactttttt agcatatagt
    26281 attcagaaat cggacttttc ggctctccta aaaggttgtt aaagataatt atttggtgaa
    26341 aaatgatgga atttcgagtg tgaacagaga gcgaactccg agaaattggc ttgaagctag
    26401 agcgaagtaa gagaaatata gtaaacggtg tttcatattg agggaataac tcatggatga
    26461 taagtactat ctccattatc actgctagtt gtgtcaccaa cctgattttc aaattcattc
    26521 ggtgcatagt aatctcttga gtcttggatt tccttaattg cagaatttaa ttcctcttcc
    26581 gtcatttcac ttgatgaaga tgtatcactt gaatcttcca caccccgagt gccacctcta
    26641 ccaccactat ttgaagtttc actgcttgac tgtgattcct gacttttgtc cgtagttgat
    26701 gaaggtgcag gctgttcttg tgtacttgtt tttgtattat ctttgctaac ttctttcttc
    26761 ttggagtctt tggtagaacc agaatttttt gatttaattt ctatcaattt ttcatatatt
    26821 ttttgtgtta aagatttctg ttggcttgct gttagatagt gagatgtcct aataacaaca
    26881 gttccaaata catagtgaga acctggatta aacatacctg cactgtcaaa tgcactcagg
    26941 tatatattac gtttttctgc ttcttctttg gttttaaaaa cctcaatatt tcctcctgca
    27001 tcggtcccct tatcgagcac atccttacct tctacagctt cagttacctg gttatcagta
    27061 aagtagattg aggcagtata accaccttgt ttatttagtt ttccattagg gtcattgctt
    27121 tctgtcactg actgaacgcc tgtaactgta tcaatctctt ttaagcgctc ctcaacaaaa
    27181 ctttgactgg gattggttat ttgggctagt tgcttaacac tatttgtata tgctaaagaa
    27241 ctatcagcta aagactgagt agcttttgaa taatctattg gttgattaag cgacttggtt
    27301 tgtttcttga tagcttcggt tttatctgct acctttggaa tttctcttaa gtcacttttg
    27361 gcagttgcta ctgctgcttt taggttgtct aaagtcctta catctagcgg ctgctggttt
    27421 gacttaacga gcttttcagc tttggcaatc tcacttttta gcgtcttatt ctttgactta
    27481 actgtctgaa cagctttatt aaagtttgtg acagcctctt ggtgaggggc gtaacttagg
    27541 aaataataag ctgaaccacc accaatagct agcaatgcga ccagcacaac aataattctc
    27601 tttttcatgt ttgtttctcc tctatgcgcg tgagttaatg cggaaaatca atatctaaac
    27661 ccgctagtcc ctctaaaata tttattatgc tttccctttc ttttggcgtt agtaaagaaa
    27721 aataaaaaat aagcttttta aaaacatttg gggtatcccc accgtcaata aattcaataa
    27781 atttctttgt ataaagcgtt taaatacaga taccatggtt ttatttatat agatgaaacc
    27841 cttgaaaata gtggcgcgtg ggttagttgt caccattgtc agatataaat ttgacaacat
    27901 ttttaacggt ttctagtttc tcaccttcca tagctagaat atccaataag gtgaaacgaa
    27961 tgtcatcacg aagtttaact atacccgtaa gcagcatgat gatatagtcg tttgtatctt
    28021 cttgaaattc tgctaaaaaa gcattgaatt tctcactgtc ttttatttcg tgttcttctt
    28081 tcattcgaat accataataa tcataatatt cttttaaatt tgaaagtatc ttatcgcgag
    28141 tatctacatt tttcaacata gatatatcca taagagtaac catgtcttgt aaatcaaaat
    28201 actctcctgt atcatctttt cgagctaatt caaagctggt tagcattggg tcttttttat
    28261 catcgctata ccccaacaga taccccacag acacgttaaa ataatccgct agggcttggg
    28321 ctttgtctgg tttgatttgg ctttcaccat tctctatttt ctgataccct cggcgactca
    28381 tattcaaaaa ctcagacata tcctgctgag tctttttttg attagctctt aattctttca
    28441 atctattcat aatttattac ctcaagtcga ttatatcata aaaagaaaac taagctaact
    28501 ttttttacac tttttagaat caccttcttg acaacgaatt ataagttagc taaaataaaa
    28561 cctgttaact aacaaaaagt tagcaaagaa aggagttgaa attattgctt attacctcaa
    28621 cacaagcaaa agctatccga agaaaacaag ctgataaaca attaacttct aaatcagcaa
    28681 gcgctgaaat tggagttaca gaagtgactt atcgcaaagt tcgtaatggc ggagaagtaa
    28741 aaaatggcat ttatcaaaaa gtcatggaat ggcttgctga agattactaa agaaaggaca
    28801 atttattgag aacagaaaca tggaatggat atgatatccg atttgtagag caccaaggcg
    28861 aatggtgggc agttcttgct gatatttgtc acgcgctaga cttaaaacca aaacgtgtga
    28921 aagagcgttt agttgatgag gtcgtttcaa ccgaccacgt cgcagacagt ttaggacgtc
    28981 aacaagaaat gctaattgtt aacgagtttg gaatttatga cactattttt tcaagtcgta
    29041 aaccagaagc aaaatcattt aagttttggg tatttgaaac cattaaacag ttaagacaag
    29101 caactggtct agaaggcttt gaggtcttta gaatgctgga taaggaacat caaaaagaag
    29161 ctatggcaag attgactaat agcttagata gggtatctaa gaaagacttg attaaagcta
    29221 acacaatcac aaataaagcc gtttccaaca agtttggata ttcaaaaatg gttaaaaaat
    29281 cagaaatgac accagatatg ttagttgcta gagaaatgat tttagatgat actgtggaac
    29341 tcatgggaat taaagaaaag ttcggattga atatcagtgt gtctgagtca atctacaata
    29401 aaaattaaaa aaagcgcaaa aatgcgcgtg aaaacaacaa aaaaaggctt accgagacca
    29461 atcagcagag cctttaacta gtataactaa actcaattaa taaagcaggc aagctattat
    29521 taaaggggtt ttagtaaaag atttgatact tccattgtat catactaccg cagagcaggc
    29581 aacgacttaa cagttgcagt tctccccgac aaaacaacaa taatcaaata acgaggtaaa
    29641 acagtgaata tcataaaaca agtaaaaagt tcttttggag aacttgaaat tgatttttat
    29701 ctggacagga atagaaatat ttttgtgacg attgaacaat tagcgcaggg atttggatat
    29761 aagagccgaa atgctattga aaagatgata gagcgccaac cctacctcaa agaaaagcga
    29821 ttttcagtta ctgacaaatt gtcagctact gatggcaaac aatacgagac ccgactattc
    29881 aataagcgag gaatttttga gatcggtatg ctgtccaaaa cggagaaagg taaaatcttt
    29941 cgtcaatgga tttatgacca tatcgaagaa ctagaaagag aaaacgctaa ctttaaactg
    30001 atacgagagc ttgaaaagtc taatcataaa gaattaacac aagctatcaa ggattgggaa
    30061 cactttaatc aatggagcta caaggctatt agcgaccttt tgctaaaatc tgtcacagga
    30121 cagactgcta aacaactgaa acagtcacgg gtaggttatg aaattgcatt agattgccta
    30181 agtgctgatg agttgacgcg atatagaaaa cttgaacaaa aagtaattgt cctattggag
    30241 ctcaatgcag aatataacga tattaaaaaa ttagtccttt aacaactgaa tagagtacgc
    30301 aaaaaagttt gatttagggg ttgacttatt aagacgactt aattataata attatgttgt
    30361 caaaactaag gaggtataca atgactgaca aaagagcagt cggcagacca ccaaagggcg
    30421 agcaaccgaa caatatcaag ataacggtta gaattagcca aggacttgat gaaaaggtac
    30481 aagcatatgc tgataatcat ggcttaacaa agccagaagc aatcagaaaa gcccttgaag
    30541 atttgtcaga ctaaacaaaa aaatagcaca gtaaccgcca acgccaattt ttggttacta
    30601 tgctatcgct cgacccacaa agtgagtacg taaatattat acatgcgtac tctatttcag
    30661 tcaacacaaa aaacagaaag ggagtgcgct ttttgtgtgc tcaaaaatca aaacaatatg
    30721 attaagaaaa aaagaaatcc gagagtacta tttaaaaatg tcgcttataa actatctgaa
    30781 attgaaggga aaacattaac agaaatcgct tcttttcttg gatttgggaa ttcagaagtt
    30841 tgcagaagta ccctatataa ttggaagcgt agaaaatggc taacattcga ccttaaaaac
    30901 ggtcattatc gcaatgttga agtattacat gaagttactc ttgaaaagat ggctaacaag
    30961 gaattaaaag aacaaggact catttataaa gctagcattt actatgaaca agtggtttca
    31021 acatccgaaa ttatagaaga cattaaaacc aaaacacaag atagaattaa agctatccac
    31081 ttacaacaaa aagcgctaga gcgtatccct agcgagttat tcgcagaatt atacactaac
    31141 atgaactaat cataaaacta gcatgaatct agtataagaa acaagcaatt acacagaaaa
    31201 taataattaa gcgagaaaaa acataatgaa atatagagta gaaacaaatc ctttttcaaa
    31261 agatagatac actcctgaac aacgagaaat gctcaaaaaa cgccaactca gcaaagataa
    31321 agcggaagcc tattttgctc gactatataa ccatcatatc gctagggtga ttattgctaa
    31381 tgtaatggca gagtatacga ctacattcag gaaaagtgcc actacttttg aagaagcatg
    31441 gggcgcttta ggttataaac aaaccacaga gattgtcttt agagccgtta acggtttacc
    31501 atgttcagag aaagacacag gggaattaga aacttattta agtgaggtat ccgcatgagg
    31561 tgtattgaag aattaaatga aatcgctaac gacattttag tagattatga aggtctttgc
    31621 ggtcaactta tggatattct aaatgtccta gatttggctg ctcaagaata tagtaaggaa
    31681 acaactagtg caattataaa cacgagttgt cgcgctttga agtgccttat ttctgaccat
    31741 gataagatga cacataaata cagaaaggag ttgtaaccat gcaagaactt aactttacac
    31801 taacacagac acttatttta ttcattgttt taggtctcat agggcttctt ctaagccgtt
    31861 ctaagccatt aatagagatt gacttaccag aagataccca agcacctaaa ccacgtcaga
    31921 atgcaaacta tggggcttat attcaagcac agaaccatta ttacaattag ggggaactga
    31981 atgacattgc ctgaaaatta tagacgtgtc cttaatctga ttaaggttgg ggcagacaat
    32041 cccattacag ggcagagatt ggcttaatac tgaaacgcga agaacgctcc gtccaaagta
    32101 tcatcagtag cttaatcacg cgctacaacg tccctattat tggcattaga cacggattca
    32161 atcgtggtta ctttatccca gctaataaag aagaattact agatggtgct aaagcctttt
    32221 ataatcaagt acaaaaggaa caacaacgcc taagtgtctt attgaatgcc gatttaacca
    32281 gttataagga attactcaaa ggaggttagt atgaacgtat ttagtcaaga ttatgaagca
    32341 aaactcttag aacaaaatct gaccgctttt aatcgcttct tagaagtata ccagcaacct
    32401 aaaccaagag ttttagggtt gatcacggct gaacaggtca aagaggaatt aaatatcaaa
    32461 ggtaaaaccc taaagcggtg ggaaaacgct gggttaaaac gataccaacc accactagaa
    32521 gacacaagga aacattatta caaggtcagt gatattctta tctttttggg ggtggatgtg
    32581 tagatggcta tttatgaagc aagaggcttt agctcttatt tgtaccccta caaaggacaa
    32641 ttagaaccgt ttgactatat cgcacagttt aaaccaatga aaacgcctga ggctatcgat
    32701 attgaagaat acaagcgaac acaagctccg tactgtttga gtggcaaggt aaccccagag
    32761 aaaaacggta gctataagcg caataatgct agtctagtct atcgtgattt gatttttctt
    32821 gactatgacg agatagaaac agacgtaaac ctgcctaaaa tcgtttctaa gacactttgg
    32881 gaatacagct atattatttt atccaacgat taaacacacc cctgagaagc cacgctatcg
    32941 ccttgtcgtg aagcctagtg acactatgga caaaacgacg tataaacaag tggtcaagga
    33001 gatagctaat aagattggac taccgtttga tttagctagt cttacctggt cgcaattaca
    33061 aggcttaccc gtcacaacag gcgacccaga agactatcag cgctatgtga accgtggtct
    33121 tgattatcct gtccctaaag cacccaaccg acaggttact actacttaca cgtcacgccc
    33181 tagaagtcag cgttctatta ccatgagggt catagatacc ttgtttaatg gttttggaga
    33241 cgaaggcgga cgtaatgtag ccttaactaa atttgttggt ttgctattta ataaatgggt
    33301 ggattgtgat ttagagacgg cttatgagct ggtacaaata gctaacagcg tgacaactaa
    33361 gccactaccc attgatgaga tagacaaaac ttttagaagc atactcgata cagaactaag
    33421 aaagagagga atcaagccat agaaaaggaa gaattgaaag attatcaaaa taaactatca
    33481 caagccactc aacctgcttt tgcccctgca ttcaggacgc gaaaaggtag aggagacaaa
    33541 gaatatgtca ttagtagccc ctacaatgtc ggtaaggttt ttgaattcta cgaaaatatc
    33601 ttcacaggta ttaaatacaa cgaatttgaa aaaactattg aaatcactaa aacactccct
    33661 tggtctaaag aaaaagggct atggacgaat gaacagacca gcctttgtat tgcattcatt
    33721 gatgataaat atcggtttac ccctcgtaaa gaacatatag aggtagctat taccgcttta
    33781 gctaaaaaga actcttacca ccccattaaa cagcgtattg aaagtcaaaa atgggatggt
    33841 aaagctagag gagaacgcta ctttattgat ctattaggct gtgctgataa ttcctataat
    33901 agagaaattg ccaaagtatg gctaacaggt cttatagcta gaatttatct ccgtaaagta
    33961 aagtttgaag tcgttcctat tctcattgat aaaagacaag gaactggaaa aagcactgtt
    34021 actaagcgct tgcttcctag ctaccacacc gattcagaaa tcaagtttgg taaaaatgat
    34081 agtgattatc agaagataca agccaatgcc atcatcgagc taggggagct aaaaggtatg
    34141 tcaaaggcag aaattgaaac ggttaaaagc ttcatttcct cagatagtga tacttatcgt
    34201 gaaccttatg aacgtaaagc cactcctcat ccaaggcact gtgtctttat cgggacagct
    34261 aacaaaaaat cttttcttaa ggatagtgga acagaaagac gcttcttccc tattgaatgt
    34321 ggtatcaatg acgtggaaaa acatcctatg gaggtggaag aagattattt cttacagata
    34381 ctcgctgaag ccaaagtatg gtttaacaat tatgaaccac taacaccatc taaagagttg
    34441 atgaatcagt tagctgatat tcaagaagat tataaagttg aagacgttga caaagaaatc
    34501 attgaccaat tactgaatga gtttcaaatt gttgaaggtt gggatagttt atcacagtat
    34561 gaacaacggc aatacgtcct aaaacagttg ggagaacctt tagataatgc tcaaagttat
    34621 agtgactacc cttctgcaca gacaaattgc ttactccagg tcacaagtcc taaccatatt
    34681 gcttatctgg ggtttaacca aaaaccaacg caaggcggta aagctcttat ttctcaaaaa
    34741 atacgtgacc atttagataa tgatgacggc tggaaaaaag gagagaatcc cgcaagaaaa
    34801 agactattta aaggtggcac tcccgtatct tactatgaac gagtttaaaa actacactaa
    34861 tactacataa aaactacacg taataacaca agccaaaaca ttggtattat aagctttata
    34921 ctctattgta gtattagtag tatttaatac tatagataat aatatttata actaatagtg
    34981 cgcgtgcaaa aaaagaaagt cttttggtca aattaatgct actaaaaaca caaaatgtca
    35041 aaaacccttg atacaactgg ctttagccct gtgttttttg ctgtgctatt tgatttttta
    35101 aatgttaata acacacattt tagagagaaa agagggacat atgaatccta actatacaaa
    35161 tcatggcata aagaaaatgc attaccatta tgatgacctg ccaaacgacc agctataccg
    35221 attggagctt cccaatggtc aatgcggtta tgacttcatc agcaaaatca atttcaacac
    35281 caagtctaaa cctggtgatt gtgattttac aagaccagaa ctcatacgcc gtggttatcg
    35341 ctgggtcttt gactgtggct ttgctatacc cgttgagaaa ggagaacaaa cacaatgaaa
    35401 atcaagctat tttatcaaaa acacaaagaa tcactggatg attttgaata tcgaatcaat
    35461 caatttaccc tatcggtatc tgtgatagac attaaatttt cagaagcaac ttatggcaat
    35521 tatgaagaca tgagtaacac aacttcttta ttggtcttgt acaggtaact aaaattacac
    35581 acacgaggcg gtaacaccgt cactatacaa ggagactgca ccctttataa tgagttggtc
    35641 aaatatcttc tatctgggca acaaccaaac tgggtagcca tcatcaattt atcagacatt
    35701 atagcaatca caaaggagaa ataacatgag aacattttca gacacaccaa aaacatttac
    35761 attccactat acttttaaag actttgatac tgcgcaagtt gcctgtcatg ccattctagg
    35821 gtatatgact gggagctatg agcaaccagt gattgacgca acttatcaca atgatgatca
    35881 aggtggtcat gctaaccagt tagtacttga atatgccgaa gacagaaaac taaataaggt
    35941 cttcaagcgt atctgtgaca gctttaagga ctattacaac caacctgagg atatgacgga
    36001 tgaagaactt gatgacgtag ctcaagaaac tgagttacta aaggaggttg aaagtactga
    36061 caagaagcgc gtggttcctt tatttgacaa tagccaagag aaagcggaca aacaagacat
    36121 attcatggct ttcatctcag accacaacca actcgccgaa cacgtttcca tgaattataa
    36181 aaagatgact caagaggatt taggagctgt acttgaatct atcagccaag gctttaatta
    36241 tttgtatgat atggctattg agggtgagtt actggttaaa taaacaatca gagggatttc
    36301 cctctttttg tcgtttatca atagttttgg gttatttgaa tactaaggag aaaagatgtt
    36361 agaactagct attgagagta tcattaaacc aatgaagaaa caggaaaaga ctagagttac
    36421 aggaacaata aatgaccaat ctatcaccat agacctagat aacctaatcg tccattatga
    36481 ggggcaagaa ttcttacttg aaacgatacc aggaacttat ggtggtaagc gctacttctt
    36541 cctgtgtcct gactgtgaga gacgttgtag aaagttattt aaggcttctc atgcctttgc
    36601 ttgtggtact tgtcagaagc ttcatcaagc gacactcaat cgaagtaaga cagattgtca
    36661 atactattgg caactagcct ttaaagagtg tctgaaagta gattcaaaag caaaacacaa
    36721 acatggttat tatagttatg atgactttcc taagcgtcca aagtacatga gaatgactaa
    36781 atacttatat cattggaaga gattccatta ctatatggat aagggggaca aatactggct
    36841 ataacattcg gaaaaacaga tagctgttat caaagctaga tacgtggatg acttatcatg
    36901 ggatgagata cccgacaagc taggttattc aaggaatact gcattcaaat tacatagaga
    36961 agctttagag gtattagatg agcaagaaga acgctattcg taaattaaaa gagtttcata
    37021 gatggcaacg tatcgccaat agccttgatt taagctataa cgagcgttac cagtttgata
    37081 taaaatatca tcccacgcgc agagaacacc ttgaaataag ccgagaatgt gccttagagg
    37141 agctagacgc tatcaagtat gctatcaacc aactgtctaa gatagactac agaaagatat
    37201 tgattgagtg ttacttgatt agtgagaaaa aaactcagca agacatcata gcagaactta
    37261 acaaaagtca aagttggtac tacgagacta agaaaagagc tttgcttgaa ttcgcccaga
    37321 gttataggaa tggtgtgtta ataacaacag cataggctct tggatatatg caactataca
    37381 ggtatagttt aaagtgttat aataactaaa aggaggtgct actatgcacg ctttagaaaa
    37441 aaatacgcag gtcaacttta aaaccaacag tgatttacta gagaaagcta aagctattat
    37501 cgcagcccaa aaccttgaca tgacagctag ctttaatttg tttttagaaa acattgtaca
    37561 aaataaagcc ttaccttttg agactgacac cgataaagaa cgagctgaac tacttgcagg
    37621 tttacgagct gaaattgcta aaagttttga cgatttagag catgggcggg tatacaacgc
    37681 tagtgaagtg agggctaacc ttggtatctg ataataaaac ccatagcctt attatccctg
    37741 aaaccgtaca agaacaatta caggagataa aaagctatat tgaaactact tacttttcgg
    37801 agcaggcagg ggctaacacc gtcaacaata ttctttacgg gctagaacgt cttgaatttt
    37861 tcccagaagc aggatttaac gcagatgaca gagtaggtga aactatttac ccgccacata
    37921 atactcgttg tattgtttta ggggactacc tagcttttta tcacatttta gaagacagaa
    37981 aagccgtttt tgtatctgat attatccata gcaaacaaga ctatatcaaa ctgtttaaga
    38041 aaaaataaaa acgcaagatt tacagcaaac taaaaagcac ctttattggg tgctagttct
    38101 tgcctgctga actcattgat tgacagagtt gtaaattaaa tttttgctta ctttttgcta
    38161 actttgaagg aaattttaat gtcaactaat gttgacaaaa aaagtttaaa atgttgattt
    38221 aaagctataa agacttgtaa agcaacctta aggaagtctc aaaaatacag tacaacaaaa
    38281 tgctttaatt attacattac atactttaaa agcctatttt ataggctttt tttgctatat
    38341 actttttagt gagtcattat atttaatgaa ccaaaaagat tttaaactaa cattagaata
    38401 aaaatcaaca ctgtcagttt gtaatgagta attttttact ttgtaaaaag gatatagtag
    38461 agttaacgtt acaaatactt gagaatgata acttatctcg tcgaatctta tataactaag
    38521 gagattttat tatgaatcta aatgatagat taaaaataga agaaatggaa gagaagtatg
    38581 atagcttcaa acctagaatc aatgcattag tagaagctat agatgatttt caaaagcact
    38641 atgaagatta cgtgaaatta agagaatttt atggtagcga agactggttt agactatcag
    38701 aacaaacaga aaataatctc aaatgcggtg tactgagtga ggatcagctc tttgatttta
    38761 taggagaaca taacgagctt gtaggccaat ttttagatat gtcttctcaa atgtatcgtc
    38821 atttataatg tcagtcctta ttagtgttgt tatcctataa tgaggataaa ataaacctaa
    38881 ctaaaaatga ccagcctata aggaaggctg gttttaagtt tgaggtaata aactgagtag
    38941 cacttgtgta atttgttgag ggctttcttt ttttcctcgt gttatccaca tttgatagac
    39001 tccaaaaata gcatgactga gatagacact ataatacaaa cggttgatat cagactttgt
    39061 tgcatctgga ttgaccacag gtaattcttt agaaagcatt aattgtaatt tattaaggat
    39121 gtaggtctga atttcttttg ttccattttg ggttaagaga gcagcaaata atgattctct
    39181 atctaagaat tgaaaaattt cgagcaaggc atcttctttt ttaaattgat tgcgatcaaa
    39241 aatatattcg actttattga agagtgactg ttggtattga tcaatcattt catatttatc
    39301 tttataatga gtataaaaac tagaacgact gattccagct gttttagtta gattaatcgt
    39361 tgaaatttca tcgaagctct gatcttttaa taacgtaatc atagctgctt caatagctct
    39421 ttttgttttt tctctcctcg tatcgtttac cagattaatt accccttaat atttttgtac
    39481 atttttaaac acagtgtcca aaaacgagat ttttatcttg aaattatcta tcatcactgt
    39541 ataatatatt ttatcaaaaa aatgaacaat atgtccaaaa taggagaaaa aatgttagac
    39601 gaattaaaag caatcataaa aagcccaaaa ttatggatta cgatggctgg ggttgcactg
    39661 attccaacgc tatataatgt tatctttcta agctcaatgt gggatcctta tgggaatact
    39721 aaaaatttac ctgtggcagt cgttaaccaa gataaatcgg ctaaattaaa tggaaaaact
    39781 atttcaattg gcaaagatat ggaggacaat ttgtctaaaa atgatagctt agatttccat
    39841 tttacaacag ctaaaagagc agaaaaagaa ttagaaaagg gtcactatta tatggttatt
    39901 acctttccta aagatctttc tagaaaagcg acaactctca tgacagaaaa accagaaagg
    39961 ttaaatatta cttataaaac tactaagggg cgtagttttg tagcttcaaa aatgagtgag
    40021 acagcagcta ataaactaaa agatgaggta gcagaatcta ttacagggac ttatactgaa
    40081 tcagttttta aaaatatggg ttctatgaaa acaggtatca ataaagctgc tgatgggagt
    40141 caagaattat taaatggttc taataagcta cagaatggga gccaaacctt gacaagtaat
    40201 ttagatgttt tagcaagctc tagtcaaact ttttctggag gagctaataa actaaatagt
    40261 ggaataaacc tttatacaga tggtgtggga accctatcta acgggttaga aacattatca
    40321 gatggtgtta ctgcttacac aacaggtgtg cataagttga gtgaaggtag tcaaaaatta
    40381 gatgataagt ctcaagcatt ggtggaagga tcagaaaagt tgacggttgg tttacaacaa
    40441 ttgtcacaag caactcaact aaaaccagag caagaacgta ctttgcagaa cctttcagat
    40501 ggattaaaga atctaaatca gattattaca aatttacaat cgacagcaac tactgatagc
    40561 gatactaata gtaagttatt taatttttta tcaactattg aatcaagtac aacggctttg
    40621 atgaatacgg ctgctgctga taaacaaaag caaatgactg cggtacaatc aacgtcagct
    40681 tttaaaagtt taacccctga acaacaatct caaattacct cagcggttac tggaacacct
    40741 acttcagctg agacaattgc agcaaatatt tcttcaaata tagaaaatat gaaaactgtg
    40801 cttagtgaag catctagttc agccccttct aataataact cacaaaattt tcaaactttg
    40861 tcaggaacag ctaacaattt agtattaaaa gctatctcag atttagataa aattcaaaaa
    40921 ttgccaacgg ctactaagca gttatatcaa ggtagtcaaa ccttgacaaa aggaatcacg
    40981 gattatacga atgctgtagg gcaattacgc aaaggtgcag aaactttaga tagtaaatcc
    41041 aatcaattga tttcaggaac acaaaaagca agtcagggag ctcaaacttt agatagtaaa
    41101 tcggatcaat tgagagatgg agcggggcaa ttagctagtg gttctgatag aattgcagat
    41161 ggttctaata agcttgctgg gggaggacat cagttgacag atgggttgac agagttgtca
    41221 ggtggtgttt ctcaattatc aagttctttg ggtaaagctg gggaccagtt gtccatggtt
    41281 tctgtaaaca aggataatgc caatgctgta tctagcccag tcactatcaa gcatgaagat
    41341 tatgatagcg ttgatacgaa tggtgttgga atggcaccat acatgatttc agttgcgttg
    41401 atggtggtag ctttatcagc caatgttatt tttgcgaaag ctttatctgg caaagagcca
    41461 gctaatcgtt ttagttgggc taaaaataaa ttattaatca atggattcat tgcaactcta
    41521 gcagcaacta tcttattttt tgcagttcaa ttcataggtc ttaaaccaga ttaccctgga
    41581 aaaacctact ttattatcct attgacagca tggactttga tggcattagt aactgcttta
    41641 gtgggatggg ataataggta tggttccttc ttgtcgttat taatattatt attccagctt
    41701 ggttcaagcg caggaactta cccaatagaa ttgagtccta agttctttca aacaattcaa
    41761 ccatttttac cgatgactta ctctgtttca ggattaaggg agaccatctc gttgacagga
    41821 gacgttaacc atcaatggag aatgctagta atctttttag tatcatcgat gatacttgct
    41881 cttcttattt atcgtaaaca agaagattaa tagaaagtat ctagtgatag actaacagta
    41941 tgatatgcta tgtcaaagta tttaggagga gaagatatgt ctactttaac aataattatt
    42001 gcaacattaa ctgctttgga acatttttat attatgtatt tggagacgtt agccacccag
    42061 tcaaatatga ctgggaagat ttttagtatg tctaaagaag agttgtcata tttacccgtt
    42121 attaaacttt ttaagaatca aggtgtatac aacggcttga ttggtctatt cctcctttat
    42181 gggttatata tttcacagaa tcaagaaatt gtagctattt ttttaatcaa tgtgttgcta
    42241 gttgctgttt atggtgcttt gacagttgat aaaaaaatct tattaaaaca gggtggttta
    42301 cctatattag ctcttttaac attcttattt taatactact tagccgttcg atttagttga
    42361 acggctttta gtaatcattt ttttctcata atacaggtag tttaagtaat ttgtctttaa
    42421 aaatagtata atataactat gaattcaaag agaggtgact ttggttatga ctgagaactg
    42481 gttacatact aaagatggtt cagatattta ttatcgtgtc gttggtcaag gtcaaccgat
    42541 tgttttttta catggcaata gcttaagtag tcgctatttt gataagcaaa tagcatattt
    42601 ttctaagtat taccaagtta ttgttatgga tagtagaggg catggcaaaa gtcatgcaaa
    42661 gctaaatacc attagtttca ggcaaatagc agttgactta aaggatatct tagttcattt
    42721 agagattgat aaagttatat tggtaggcca tagcgatggt gctaatttag ctttagtttt
    42781 tcaaacgatg tttccagata tggttagagg gcttttgctt aattcaggga acctgactat
    42841 tcatggtcag cgatggtggg atattctttt agtaaggatt gcctataaat tccttcacta
    42901 tttagggaaa ctctttccgt atatgaggca aaaagctcaa gttatttcgc ttatgttgga
    42961 ggatttgaag attagtccag ctgatttaca gcatgtgtca actcctgtaa tggttttggt
    43021 tggaaataag gacataatta agttaaatca ttctaagaaa cttgcttctt attttccaag
    43081 gggggagttt tattctttag ttggctttgg gcatcacatt attaagcaag attcccatgt
    43141 ttttaatatt attgcaaaaa agtttatcaa cgatacgttg aaaggagaaa ttgttgaaaa
    43201 agctaattga aaaagtcaaa tcactgactt ctgtgattaa aattgtattt tttatatctg
    43261 ttttagtgct tattattgtt gaaatgattc atttgaaacg aactatttct gttgagcaac
    43321 taaagagtgt ttttgggcaa ttatctccaa tgaatctttt cttaattatc cttgtggggg
    43381 ttatcgctgt cttaccgaca accggatatg actttgtact gaatggactt ttacgtacag
    43441 ataaaagcaa aaggtatatt ttacagacta gttggtgtat caacactttt aataacttgt
    43501 caggattcgg tggcttaatc gatattgggt tgcgcatggc tttttatggt aaaaaaggtc
    43561 aagagaagag tgacctaaga gaagtgactc gttttttacc ctatcttatt tctggtctgt
    43621 catttattag tgtgattgcc ttaatcatga gccatatttt tcatgccaaa gctagtgttg
    43681 attactatta tttggtatta attggtgcta gtatgtattt tcctgttatt tattggattt
    43741 ctggtcataa aggaagccat tatttcggag atatgccatc tagtactcgt ataaaattag
    43801 gtattgtttc tttttttgaa tggggatgtg cagccgcagc gtttataatt atcggttatt
    43861 taatgggcat tcatctacca gtttataaaa ttttaccact attttgtatt ggttgtgccg
    43921 tcgggattgt atcccttatt cccggtggat taggaagttt tgaattagtt ctatttacag
    43981 ggtttgctgc cgagggacta cctaaagaaa ctgtggttgc atggttatta ctttatcgtt
    44041 tagcctacta tattattcca ttctttgcag gtatctattt ctttatccat tatttaggta
    44101 gtcaaataaa tcaacgttat gaaaatgtcc cgaaagagtt agtatcaact gttctacaaa
    44161 ccatggtgag ccatttgatg cgtattttag gtgcattctt aatattttca acagcatttt
    44221 ttgaaaatat tacttatatt atgtggttgc agaagctagg cttggaccca ttacaagaac
    44281 aaatgttatg gcagtttcca ggtttattgc tgggggtttg ttttattctc ttagctagaa
    44341 ctattgatca aaaagtgaaa aatgcttttc caattgctat tatctggatt actttgacat
    44401 tgttttatct taatttaggt catattagtt ggcgactatc tttctggttt attttactat
    44461 tgttaggctt attagtcatt aagccaactc tctataaaaa acaatttatt tatagctggg
    44521 aagagcgtat taaggatgga atcattatcg ttagtttaat gggagttcta ttttatattg
    44581 caggactact attccctatc agggctcata ttacaggtgg tagtattgaa cgcctgcatt
    44641 atatcatagc atgggagccg atagcattgg ctacgttgat tcttactctc gtttatttat
    44701 gtttggttaa gattttacaa ggaaaatctt gtcagattgg tgatgtgttc aatgtggatc
    44761 gttataaaaa actacttcaa gcttacggtg gttcttcgga tagcggttta gcctttttaa
    44821 atgataaaag gctctactgg taccaaaaaa atggagaaga ttgcgttgcg ttccaatttg
    44881 taattgtcaa taataaatgt cttattatgg gggaaccagc cggtgatgac acttatattc
    44941 gtgaagctat tgaatcgttt attgatgatg ctgataagct agactatgac cttgtttttt
    45001 acagtattgg acagaagttg acactacttt tacatgagta tggttttgac tttatgaaag
    45061 ttggtgagga tgctttagtt aatttagaaa cgtttactct taaagggaat aagtacaaac
    45121 ctttcagaaa tgccctaaat agagttgaaa aggatggttt ctatttcgaa gttgtacaat
    45181 cgccacatag tcaagagcta ctaaatagtt tggaagagat ttctaatact tggttagaag
    45241 gacgtcctga aaaaggtttc tcactaggat attttaataa agattatttc caacaagccc
    45301 caatagcttt ggtgaaaaat gctgaacacg aagttgttgc ttttgctaat attatgccaa
    45361 actatgaaaa gagtattatc tctattgatt taatgcgtca cgataaacag aaaattccga
    45421 atggcgttat ggatttcctc tttttatcat tattctctta ttatcaagag aagggatacc
    45481 actattttga tttggggatg gcacctttat caggagttgg tcgcgttgaa acaagttttg
    45541 ctaaagagag aatggcgtat cttgtctatc atttcggtag tcatttctac tcatttaatg
    45601 gtttacacaa gtataagaag aagtttacac cattgtggtc ggaacgttat atttcttgtt
    45661 ctcgttcgtc ctggttaatt tgtgctattt gtgccctatt aatggaagat agtaaaatta
    45721 agattgttaa ataagcttta tttggcaatt aaaaagagca tgtcatgcga catgctcttt
    45781 ttaaatcatt taataccatt gattgcttga atctacttta taatatgatg tgcttttaaa
    45841 tattgtttag ctactgtagc tgctgattta tgctttacag ctacttggta gttcatttct
    45901 tgcatttctt tttcagtgat atgaccagca agtttattga gagctttttt tacttgaggg
    45961 tattttttaa tcgtatcttg ccttagtagc ggtgctcctt gatatggcgg aaatagatgc
    46021 ttatcgtctt tgagaatttg aagttgatac tggattaatt cactatcggt agagtaagcg
    46081 tcaataatat tgacatcttt actattgata gcttggtatc gtaaagcagg ttctaaggtt
    46141 gagatatcaa ggtttaaacc ataatgtttc tgtaaaccaa tactcccatc ctctctatcc
    46201 ttaaattcta aagtgaaacc agcttttagt ttatctaatt tttttaggtc gctgatattt
    46261 ttgagttgat tagcttcagc gtaatctttt ttaacagcta cagcgtaggt gttttgataa
    46321 gccataggtg acaaaagtga caacttatct tgttttaaaa taccgttttt ggctaagttg
    46381 taaacctgtt tagggttatt acttacttta ggaggatttt taagtaaact tgatgtaata
    46441 gtacctgtaa actctgggta aagatcaata tcaccagatt ttagggcttg atataagaaa
    46501 gttgtttttc cgaaattagg tttaagctta actttgatat ctgtctgatc ctcaataagt
    46561 tctttgtaca tattaattaa aatatctggt tcagttccta acttccctgc aacagttatc
    46621 gttgcagtat ttgttccagg catccatttt ggagcatagg atagtcctaa gcctgctagt
    46681 aaaagtatac cagaaacggc tatggttcgt aatctagcct tttctagtaa tccaattaaa
    46741 ccactaaata taatagctaa gacagcagaa gagattgccc ctatgagaat tagagaagga
    46801 ttgttgcgat caatccctaa caaaataaat gatccaaggc caccagctcc gataagacta
    46861 gctaatgtag ctgtaccaat aatcataaca gaggcagttc taatgcctga gatgataaca
    46921 ggcatactta aagctaactc aaacttttta agcctttccc aacgtgtcat tccaaaagcg
    46981 gttgctgcct caatgagatt agcatcaatt tgcatcagtg ctgttacagt attttgtaaa
    47041 attggaaata gagcgtaaat aattagtgca acgacagctg gtacagttcc aattcccata
    47101 aaaggtataa ataaaccaag caaggctaaa gaaggaattg tttgaaatat cccagttatc
    47161 tgaaggacaa catgactgat ttttttataa tgactaatga tgataccgag tggaatagca
    47221 attaaagttg ctaaaataag tgttagtagt gatagctgga ggtgttcaat aagactttga
    47281 gtccaatctc caaaacgctc ttgaaaggta gtgattagtg ttgtcatact cagttctcct
    47341 cttcaaatag ggttctaaca aattggtcag agggattatt ttgaattatt tctggtctat
    47401 ccaattgcac tatcttacct tccttaataa cacaaattct atctgcaaga cgcatagctt
    47461 ccttcatgtc atgggtgaca aacactaatg taatacccaa ttcagactgt aattgtttgg
    47521 tgatatcttg taattgacgt cttgagatag ggtctaaagc agaaaatggc tcatccataa
    47581 gtagtacctt aggtttagca acgattgctc gtaggatacc tatgcgttgt tgttccccac
    47641 cagagagctc atgaggatag cgattaaagt agtccttagc aggtaaaccc accttatcaa
    47701 gaaggtcgct agcggctttt ttttggtcac cttttgacca gcccttcatt tcagggataa
    47761 gttctatatt ttctccaact gttaaattag ggaaaagtgc aatttgttgc aagacataac
    47821 cagtttctaa gcgtaattgt cgcaaatcaa gtgatgtaat gttttcccca tctaaagtaa
    47881 tagcgccttg gcttggttcg atgagacgat taatcatctt gaggatggta gttttaccac
    47941 tgccactggc ccccacaagg acaaagaatt ctcctctttg aatatggcaa ctaatattat
    48001 caatgatggt atgatcacca tatgatttac taacattttc aaatgaaatc atgtaattta
    48061 ctttctagtg cgtaacgagt gtaggtcgta tgatattagg gttatattag cataaagtag
    48121 acattcaaac aactatctga catttaaatt gtcagaaacg ataaactaga agcaaaaaca
    48181 aacaccttgc ggtagacaag gtgtttgttg gagatttatg aaaagaaaag ttttaggatg
    48241 gttatagtat ataaggccat gcttaaacaa gtcttaaaac gttttactct gtaaaataag
    48301 ttcatcgaaa aaacgtttaa gtgaaatttt accaccatac atgctttttc cttgaataag
    48361 ttgtgcctca ttatggatat tctgaaatcc aaacaatgca ttagcatatt gcagtgttaa
    48421 ttcattttca aaatcatcaa tgtacagatg agccatattt atcatagctt ttttaacggc
    48481 acgacgaata cgttgtaaaa tgattttttg ttggtgttca tcgatagaaa gttctttttg
    48541 aaaatcaaat tgggcaaaat ttaattcttg ttttaataaa agttcacaga tgttaagaat
    48601 atctgtgtag gctgtctctg aagtaatacc taaataactg agtattgaac ggattttagt
    48661 gaggttacta gtcgttatag gcttttgata tgatggcgta ttttctagga gattttggat
    48721 tgtatttaat tttttttgca tttcgatagt atcagtgaca cgtttcacga cgctttttac
    48781 ttcaataata ttaatgggtt tattaataaa aaattcgatt cctgctttat atgcttcttg
    48841 tcgtaaatca ttatctttga cttgggaaat cataataaat tttaaatcgg aacgctgttt
    48901 atagattttt tggactaatg ttacaccgtc taatattggc attaataaat caatcaatac
    48961 gatatcaaca tctgcaatca gcaattcgtt gtatgcttta ctggagacat tattcactct
    49021 gactactgta ttattgaaat cttcttcaat aatatcttgt aaaatcatag tgatagtggg
    49081 gtcatcatca ataatataga atttcacgta ttatctccta acttaaatcg atttttacgg
    49141 aataacagtg taaatgttgt tccatttggc tgattaggac aaacagttat cgttccttga
    49201 tattgctctt ccattaaaat acgcacgtgg gatagcccga ttcctcgata gatatcacca
    49261 ttagcatcaa atttagtcga aaatccaggt ttgaaaatca tgtgtttcat tttatcaggt
    49321 attcctggtc cgttatcact aatctcaatt ttaataaaat cttctaattc ttctgtgtga
    49381 acactgattg tacccttttt ttgtttatca atagcttcta ctgcatttaa aacgatattg
    49441 gaaataatgg ttagtaaata gtaatagttt gatattatca aattacattt attattttcg
    49501 agaataatga tatcttgatg ccgaaattga ataatactcc tagtataaga taaaacaatt
    49561 tgaaagatat ctttcaatgc catagttgat tcatttttta cactaaaata ggttcctaag
    49621 cctttaataa tattttggta atctttctta acttcgtgaa cgtcccgcga aatatctaga
    49681 gagaggtgtt ggtattcttt aggtaggtga tatttggata actctttatc taataaataa
    49741 gcatttttca tgatgttttc aatttcaatg atattttttt gcataaaata aacctcgctt
    49801 ttaacggctg aagttgacca cataaaataa tagtatcgac gttcgtgatc gtctttttgt
    49861 aagagaagtg atagataact ataagtataa gccacagcgc aactaataaa agctcgcaaa
    49921 agtgctatgg caaataaagt agcgaatata gttatagtat aattgttaaa ctttatgaga
    49981 aaactaattt ctacaaggtt ggcaaaataa tcacaaataa tgatagaaaa gaaaaaagta
    50041 cctttattac ggtaggaacg atgccaataa atagtataaa aggtgatgcc ataacaaata
    50101 taaaacgcca tatcagttaa aacgaattca attatttgat gaatctcagc ctcgccagcg
    50161 atacttaata tgattcctct gaagatagga gaagcaaaag taattcctag acagagaaga
    50221 attggattaa tgtcgtcatt aaaatataaa aagacaggca aaatgaacaa agataaggtc
    50281 ataataaacc catcagcaag tattgagata ttgatttgag cagctaaggc gatagctatt
    50341 gcagagataa taatacgctg ccttctttct agatgttttt ctgataatat atctagcata
    50401 taaatgtcct taaagttttt ttgaagattt ttgatgaatt ttgattattt ttgaaagcgc
    50461 tatctacaat aagagtataa aaaaaaagga ggcaataagc aatgaaaaat ttacgtaacc
    50521 gtagttttct tactttattg gattttagta cagcagaagt tgaatttctt ttaaaacttt
    50581 cagaagattt aaaacgtgct aaatacgcag gaattgaaca acaaaaactt gttggtaaaa
    50641 atatcgcttt gatttttgaa aaagattcta cacggacacg ttgcgctttt gaagttgctg
    50701 ctcacgatca aggagctcat gttacttatt taggtccaac aggcagtcag atgggtaaaa
    50761 aagagacctc aaaagacact gcgcgtgtgc taggtggtat gtatgatggt attgagtatc
    50821 gtggattttc tcaagaaaca gttgagactt tagccgaatt ttcaggagtt cctgtgtgga
    50881 atggtttaac cgatgctgat catccaacgc aagtactagc cgattttctg accgctaagg
    50941 aatgcttaca taagccctat aaagatattc gctttacgta tgtgggtgat ggacgtaaca
    51001 atgtcgctaa tgccttgatg atcggtgctt ctattgtagg catgacttat catttggttt
    51061 gtcctaaaga attagagcca gatcctgaac tactctctaa atgtcaagaa attgcgaaaa
    51121 ctactggtgc ttctattgaa atcactgctg atattgcaga aggcgtcaga gattcagacg
    51181 tcctatatac agatgtttgg gtttcaatgg gagaaccgga tgaagtttgg aaagaaagaa
    51241 tagctctttt agaaccctat cgtattaccc aagaaatgct taatatgaca gaaaatccaa
    51301 acgttatttt cgagcattgc ttgccctcat ttcataatat agatactaaa gttggatacg
    51361 acatttatga aaaatatggt ttgaaggaaa tggaagtatc agatgaagtt tttgaaggcc
    51421 ctcactcagt tgttttccaa gaagctgaaa atcgtatgca tactattaaa gcagttatgg
    51481 tagcaacttt aggtgattag gaggaatttt catggcaaaa attgttgttg cactaggagg
    51541 aaatgcctta ggaaattctc cagaagaaca attaagatta gtaaaacata ctgcaaaatc
    51601 attggtggcc ttgataaaaa agggacacga aattgttgta agtcatggta atggaccaca
    51661 agttggtgcc attaatttag gaatgaattt tgcggctgag agtggtcaag gaactaattt
    51721 tccttttcca gaatgtggag caatgagcca aggatacata ggctatcatc tccaacagag
    51781 ccttttaaat gaattacgtc aagagggaat aaataaggaa gttgctacaa ttattaccca
    51841 aattgaggtg gatgaatcag accaagcatt tagcgcacct accaagccta taggcacctt
    51901 ttatgataag gagacatcag aaaagattgc tatagagaaa gggtatactt tcgtagaaga
    51961 cgctggacga gggtatcgtc gtgttgttgc ttcacctgaa cctaaaaaaa tcatcgaaat
    52021 taatagcatt aaaacactta ttgaaaatga taccctagtt atcgcaggtg gaggtggagg
    52081 catccctgtc attaataagg gaggttatga aggtattgca gctgttattg ataaagacaa
    52141 atctagtgca ctgttagcag gagaactagc tgcggatcaa ttgattattc tcactgcagt
    52201 tgattatgtt tatactcaat ttggtaaaga aaatcaaaaa gctttaacag aggttaatga
    52261 gaatcaaatg attgattacg ttaaccaagg tgagtttgca aaaggaagta tgttacctaa
    52321 agtaatagcc tgtatgagtt tcttagatca caatccgaaa ggaatagcct taatcacctc
    52381 attaaatggt ttagaagatg cattagatgg gaaattagga acacgtataa ctaagtaatc
    52441 tcagataagt gcaaagaaat gattatttaa ccttcatctt tgcacttttt ctgatgaagt
    52501 caatctattt ttattataga ggaggcgtgt catgccagaa aaaatgacaa acagtgaaaa
    52561 caaaaggagt aaaggttttc gaatgccagg tgcctttaca atattattca ttttaacaat
    52621 cttttcggtt ttggctacat ggtggatacc tgctgggtcc tattcaaaat tacaatttga
    52681 tacagcatca tccaaactag tagtaacaga tccaaatggt aagacagttc atgttccagc
    52741 aacgcaaaca cagttggata agatgaatgt caaaattaaa attaaagaat ttacttctgg
    52801 tgctattagt aagccagttt cagttccaaa tacttacaaa cgtttgaagc aaaatccagc
    52861 aggcattggc tccgtgacga ctagtatggt aaatggaacc attgaagctg ttgatattat
    52921 ggtatttatc atggtacttg gaggcatgat aggagtagtt agaaagagtg gcgcttttga
    52981 gtctggtctt ctagctttaa caaagaaaac caaaggtaga gagtttttat tgatattttt
    53041 agtttcctta ttgatggttt taggcggtac tctatgtggt attgaagaag aagcagttgc
    53101 tttttatcct attttagttc caatcttttt agccatgggt tatgattcta tcatttgtgt
    53161 aggtgctatt ttcttagcaa gttctgttgg gacatcattt tcaacaatta atccgttttc
    53221 ttctgttatt gcttcaaatg cagctggtat tagtttcaca gaaggcttat catggcgtac
    53281 agcagggtgt attgcaggtg ctatttttgt tgtggtgtat ctacattggt atgctaaaaa
    53341 aataaaagcg aatccagagt tttcatattc ctatgaagat agagtagaat ttaatgctaa
    53401 atggggcatg acaactaacc atacaccctc actctttact attagacaaa aaattatttt
    53461 aagtttattt gttatttcat ttcctttgat ggtttgggga gtaatgtcac agggatggtg
    53521 gtttccaacc atggcttctt cctttttagc tattacaatt attattatgt ttttaacagc
    53581 aactggggca aatggtattg gagaaaggga tgttgttgat gagtttgtta atggtgcatc
    53641 aagccttgtt ggggtttcat taatcattgg tttagcgcgt ggtatcaata ttattttgag
    53701 ccaaggttat atatcagata cgatgcttta cacagcttca aaattagctt cacatgttag
    53761 tggccctgtc tttatcattg ttatgatgtt tatctacttt gtgttaggat ttgttgttcc
    53821 atcctcgtcg ggtttagcag tactttcaat gccaattcta gcacctcttg cagatactgt
    53881 tgggattcct cgttcagtag tagtaatggc ttatcaattt gggcagtatg caatgttgtt
    53941 tttagcacct actggactag ttatggcaac cttgcagatg ttagatatga agtactccca
    54001 ttggctaaaa tttgtgtggc cggttgtttt attcctccta atatttggtg gcggtttgtt
    54061 agttctacaa gttttaatac tttaaagatt tcagctatta gtctcaaaaa tatctgaaga
    54121 gatataataa attgagttta agttaaataa aaaaaacgat aggtgtaatc tatcgttttt
    54181 tttatttcct ttctctagtc tttcttcaat tttggaaagg tgagcgtaaa ctgactgcca
    54241 tcatcaggtt tagaatcaac ccatatttta gcacggtgta ttgtagcgat ttgttttact
    54301 aaagatagcc ctaaaccaat tccagactga ctatccttgg ttcgagcaga gtccacttga
    54361 tagaaacggt tccaaatttt actttggtca tcaactgaga tacctagacc attatcttta
    54421 atagagaaga caatttgatt atcggactgt cttagactaa tagaaatatg attgtttgta
    54481 aatttaatag catttgaaaa tagattatca aaaagccgtt tgagtagtgt ttgttgacct
    54541 ataatccaaa ggtcatcttc gatttgagag tcaaggtgta taggggtatt atctaataat
    54601 ctactcgaat cggatactag ttgcttcatc ataattgaga aattaagagg ttctaactga
    54661 attgataatg gattttctgc cttagtcaat tctagaagtt gctcaactaa ttttttcatt
    54721 gatagtgact gacggtgtat aacctcaaag gattcttttg cttcacttaa attttctgcg
    54781 tagttttttc cgtattcact ttcagaaagt ataactgtta caggagtccg tagttcatga
    54841 gaaacatcat tgttaaattg tttttcccga acaaatgatg attcaataga agccaacata
    54901 gtattgataa cagctgctag ctcagttaat tcattttcgt tatttttagt gataattcgc
    54961 ttagtatagt cttcattttg ggtaataaat tgagcggttt cagtaatttc ttgaatcggt
    55021 ctaaaagcgc gctttaaaat taaatagcca ccaacagtta ttataatcaa cataagagga
    55081 agaacgatac ccaaactaaa taacaattca gttaattgct tatccagtct gtcaacagta
    55141 cggatagcac gtaaccactg atttttccca gaaattggaa tatcaacata ctgaaaggta
    55201 cgattctcca tggaaaatag agataatttc tttttatgtt tatgttgatg gtctaaatcc
    55261 cttttaaatc cttttggaaa accagaatag ataacttttc cattttggtc atagactgaa
    55321 taaaatattc catcttcaaa agcctcatac cgatgacctt tagctaacgc ttgactcatt
    55381 tgcacagctt tagcttctaa atttttttta gatttgtttt cagcaatgct ttttgaaata
    55441 aaaaatgaac caattaatac accgataaca aagataaaaa taaaagagct gtaccagagg
    55501 gtaatttgag tgatgatgct agacttttta gggtaattta ttttactttg gaataacata
    55561 tcctaaacct cgtttagttt ttattagtga tccatctcga ttgttatcta gttttcgacg
    55621 aaggtttttg ataaggacat caataatatt ggactctcca taataatcat aatcccaaac
    55681 gtgttcacga atttgatgac gagagaggac ttggtctctg tgacgtgcca aatactcaag
    55741 gacttcgtat tccttagctg ttaaatcaat caaattgtcg tttctccaaa cttgctttgt
    55801 agataaattg attgttatat cttgaatttg aatatcatca gaagatactt gtctatttgt
    55861 acgtcgtaac atagctcgaa ttcgggccat gagttctcca aattcaaatg gttttacaag
    55921 gtaatcatcc gcaccaaaat ctagacctgc gatacgatct tcaacggcat ctcgagcagt
    55981 caacataaga atagatactt gacttccttt gttgcgaagc agttttaaaa aggtgaagcc
    56041 gtccattttt ggcatcatta catctaagat aatcacatca tattctgcca ctgaaacaaa
    56101 ttgtaaggct tcttcaccat ttgaagcact gtcaacactg tagtgttgct tttttaaaag
    56161 ttttacaata ctacggttca agtcaaactc atcttcaaca acaaggattt tcattttttg
    56221 gcctccgtat ggcttttttc cattatatca caaaagggaa tggagtactg aggtcctatt
    56281 ccctttaata cactagtatt agtcgtgatt tttatgagtt gtattaagct tatttgcctt
    56341 tataaatgat gctttatcac tatcaaattg ggcaatctta ggagtatttc catcaagtag
    56401 ttttacttta taaactagct tatttgattt gttattcgca agagaaattt cttttatttt
    56461 tgcattttta tattttgctt gaatagcttg taatgcagtc tgaatatctt tacttggttg
    56521 ttttaattgg gcatcttttg aattgagttg attatctgat gttgttgaaa ggatcttccc
    56581 agaattagca tcaattttat attttttagc tattttatta tcaataactt tgatttcata
    56641 aaaaggtgta tcacctttgg tatttaattc aatttgtgaa ataaagcctg agcgagcttc
    56701 ttttttggca gtttctaagg cagtcttata atcgattttt gtcgagatat tctttaatgt
    56761 ttttagtgag tatttttctc tatgatcgtg atgtttatcg tgtccttcat gcttatgttc
    56821 aaaagacatg tgttctgagt gatggttatg cgctaatgca gctccactta ctcctatagc
    56881 tcccccaata ccaagtagcg taatggaagc taaccctacg tataatttat tgctcatgaa
    56941 ttttttaaag ttgtttttat agtttgtcat actgacttcc ttctttcttt tttcaaacct
    57001 tggattaggt t
//
LOCUS       DATRME010000006        51111 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRME010000006 DATRME010000000
VERSION     DATRME010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51111
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..950
                     /locus_tag="VBC23_000350"
     CDS             <1..950
                     /locus_tag="VBC23_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6842324.1"
                     /translation="TTVSFFLNQKFEKMSDETRQRIQEVIDETGYRPSTIARSLNSKK
                     TKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSNYDAKSEEDYIENMLNLGV
                     DGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWVKANNYDAVYDMTQECLNR
                     GYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLVIEDDDHSKSDIEDFLKAV
                     VPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFDNIEWTDFSSPKVSTIVQP
                     AYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(1022..1636)
                     /locus_tag="VBC23_000351"
     CDS             complement(1022..1636)
                     /locus_tag="VBC23_000351"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6842325.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(1702..2346)
                     /locus_tag="VBC23_000352"
     CDS             complement(1702..2346)
                     /locus_tag="VBC23_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6842326.1"
                     /translation="MDIENYCQDYCLETIFLKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            2458..3621
                     /locus_tag="VBC23_000353"
     CDS             2458..3621
                     /locus_tag="VBC23_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6842327.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            complement(3667..4650)
                     /locus_tag="VBC23_000354"
     CDS             complement(3667..4650)
                     /locus_tag="VBC23_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6842328.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            4798..6693
                     /locus_tag="VBC23_000355"
     CDS             4798..6693
                     /locus_tag="VBC23_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6842329.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(6743..7696)
                     /locus_tag="VBC23_000356"
     CDS             complement(6743..7696)
                     /locus_tag="VBC23_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6842330.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            7863..8099
                     /locus_tag="VBC23_000357"
     CDS             7863..8099
                     /locus_tag="VBC23_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842331.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            8101..10170
                     /locus_tag="VBC23_000358"
     CDS             8101..10170
                     /locus_tag="VBC23_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO6842332.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(10314..11381)
                     /locus_tag="VBC23_000359"
     CDS             complement(10314..11381)
                     /locus_tag="VBC23_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6842333.1"
                     /translation="MAYYRKRDNGWEYRISYKDESGKFRQKSKSGFKTKKLAQAAARD
                     IEKKLSQNILTDGEVTLYDFVKTWSEVYKRPYVKDKTWETYTKNFRHIKTYFKDIKVK
                     DITPLYYQKRLNEFGEKYAQETLEKFHYQIKGAMKVAVREQVIHFNFADDAKVKSQIE
                     SRAEENDFLEESEYKALLSLTRENIQYVSYFTLYLLSVTGLRFSEVMGLTWSDIDFKN
                     GILDINKSFDYSNTQDFADLKNESSKRKVPIDSNTIDILREYKKNHWQANIKNRVCFG
                     VSNSACNKLIKKIVGRKVRNHSLRHTYASFLILNGVDIVTISKLLGHESPDITLKVYT
                     HQMEALAERNFEKIKNIFLVA"
     gene            complement(11505..11909)
                     /locus_tag="VBC23_000360"
     CDS             complement(11505..11909)
                     /locus_tag="VBC23_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEO6842334.1"
                     /translation="MALFGGKDKEIEVELFTSEPNERVFEFKKSKTIVRIDDYFIRIA
                     RKSNMSNMLLHGLDGEKSILLSEITAYQLKEPSSTVGYLQLVYPGSSDTKGGVFDAVK
                     DENTVTFSKDEKIAILELKNAIENALKNKVKK"
     gene            complement(11931..12677)
                     /locus_tag="VBC23_000361"
     CDS             complement(11931..12677)
                     /locus_tag="VBC23_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6842335.1"
                     /translation="MKYTPLSREQYFDYHNKAYQIISSIDKMKENVSYQDVIKHFEQN
                     YPILFNFLDYDEMKERYPELPDYHPTDKDIKYRGLVSNRTVTYTDRVLCESCAGLTVP
                     DLDLGRYIIYINQHTNTKGRVIFTILHELSHIHCHLNQTENQPIYMSLMSKNASEKYP
                     KELIPIEKEADTVASILYLTDERLKKALTTRESFEDIQRETDISKPALHNRLMNYLIY
                     NLQYAESYALKLVLNYRQGGGQIFNILRLQ"
     gene            complement(12686..13066)
                     /locus_tag="VBC23_000362"
     CDS             complement(12686..13066)
                     /locus_tag="VBC23_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842336.1"
                     /translation="MFFTFEKIKELADKQGISLNQLEEQLDFPRNTIYNMKKSTPNVE
                     RVSKIADYFNVSTDYLLGRTENPKIADGQDNTAVDDLTQQAIVLFRKETEGLSASEKE
                     RFNSALEGLMKTARQLIQDDSNWK"
     gene            13354..13518
                     /locus_tag="VBC23_000363"
     CDS             13354..13518
                     /locus_tag="VBC23_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842337.1"
                     /translation="MGIGSMTVTVKVTNMDKFIELSKEFNKKARELEELAHELKSFDF
                     EGEVVSTDSN"
     gene            complement(13501..13971)
                     /locus_tag="VBC23_000364"
     CDS             complement(13501..13971)
                     /locus_tag="VBC23_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842338.1"
                     /translation="MNLNQIRIIEACHKFLIGITNFEEELQDDVLVYRYKGNLVSFET
                     YQEYEQRSFVDYNLKYGYLDDTRTYIDDRADLIAAFPSEEHLRALQRVNDAEQARVQI
                     FKLLSQVNLDSLSEKNSHIKKDNFGYDFFNFATKEEYPVYLFSDDESFELVAIS"
     gene            14032..14244
                     /locus_tag="VBC23_000365"
     CDS             14032..14244
                     /locus_tag="VBC23_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842339.1"
                     /translation="MSQQQKKWIQLVKDKLNEEQMTQTHLARACGVAKATISELLKYG
                     KGSVRLKNKVSDILHIDESWTDLEED"
     gene            14244..14585
                     /locus_tag="VBC23_000366"
     CDS             14244..14585
                     /locus_tag="VBC23_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842340.1"
                     /translation="MPITREMTITEINVLNAIKNSATYDLPIQARELRQQLGLSKRSL
                     EAVIENLRVIYKQPIVAKKKQPSGYYLPRNEEERNDGLAPYKRQILTEQNNLAAIMSV
                     NLEEYWATEKA"
     gene            14632..15162
                     /locus_tag="VBC23_000367"
     CDS             14632..15162
                     /locus_tag="VBC23_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6842341.1"
                     /translation="MNEVWKDIPIVKGYYQVSNLGRVRSIGRTVNAKQRTRKTKGRIL
                     KQSLSSGYAIVTLSVNGLRKSIRVHRLVAEAFIPNPINKRTINHIDENKLNNRVDNLE
                     WATDKENANHGNRTTKSSLGRCKPVEQFTLEGEFINTFDSIKSASMKTGISSQRITAT
                     AMGHQKQTHGYKWRYA"
     gene            15162..15446
                     /locus_tag="VBC23_000368"
     CDS             15162..15446
                     /locus_tag="VBC23_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6842342.1"
                     /translation="MNDLMIQMLDQFEAGLMDRTMKVMHVVTDEKYRFPLELNKSQCA
                     LMLLGTKDTGTFDERFNCHKDFPRIPNAREKYPRDEVIEWYHKNWQRTVI"
     gene            15470..15688
                     /locus_tag="VBC23_000369"
     CDS             15470..15688
                     /locus_tag="VBC23_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842343.1"
                     /translation="MNNLQIIAVGTLISVTLIESLISNIKLRKALKNKQTAGTVIAQY
                     IPQRGFIDYKTGRRVDIDPETKKEVFVD"
     gene            15697..17028
                     /gene="dnaB"
                     /locus_tag="VBC23_000370"
     CDS             15697..17028
                     /gene="dnaB"
                     /locus_tag="VBC23_000370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6842344.1"
                     /translation="MDELKVLPHDIQAEQSVLGSIFIKPEKMIEVAEYLKPNDFYRPA
                     HKILFKAMVSLADRGEAIDIVTIKSTLESTDELGMVGGISYIAEIVNAVPTSSHAEHY
                     AKIVAKKAQLRSIIDNLSDSIGNAYDEDMDIDEIIAKAERSLIEVSQASNKSSFRPIH
                     DVLLENHSKIEERSNNTSQITGIETGFYDFDKLITGLHEDQLIVLAARPAMGKTALAL
                     NIAQNVATKSNKAVAVFSLEMGAESLVERMLSAEGTIINHHIRTGNLTVNEWQRLIYA
                     QGQLAEAPIFIDDTAGVKITDIRARARRLSQETDGLGLIVIDYLQLIQGSRSDNRQQE
                     VSEISRQLKIIAKELKVPVIALSQLSRGVEQRNDKRPIMSDLRESGSIEQDADIVAFL
                     YRDAYYQDKKEGQPENDITELIIRKNRHGNLGTVKLYFHKEYTKFSSVEEE"
     gene            17030..17632
                     /locus_tag="VBC23_000371"
     CDS             17030..17632
                     /locus_tag="VBC23_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842345.1"
                     /translation="MKKFDHYFSNHIAVKNESDTVTIVDYYNPEKGYEYNLRYIFDRK
                     NSSLAITGDFGELVARNFYNMGEWDTFYNHYTGHLGYFLEKLLCSSRPVHYYDTDKAK
                     REILNEFFDGADYDDLDFDDEFLLDQLFDNFDEDKGFTYILPEAFERFSEDMEEYEVY
                     EFLSRAGRYVNDVFELYLDAYKRAYEYLTKEVDDGTTQNV"
     gene            17610..18356
                     /locus_tag="VBC23_000372"
     CDS             17610..18356
                     /locus_tag="VBC23_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HEO6842346.1"
                     /translation="MAQRRMFSRKITETDRFLEMPLSSQALYFHLNMGADDEGFIDKA
                     KTIQRTIGASDDDMKLLIAKGFLIPFDSGVVVIRHWRIHNYIQSDRFQSTLYQSEKAQ
                     LEYDKSKTSSLKPIGNCIQNVSKMETQVRLSKGSLDKDSLTTYPTVSDNEEEDIPYKE
                     IISYLNEKANRNYRPNIQKNKTLIKARWSEGFRLDDFKHVIDTTVKDWSGTKYEKYLR
                     PETLFGSKFEGYLNQAPRIKTETIDGRLGF"
     gene            18357..19178
                     /locus_tag="VBC23_000373"
     CDS             18357..19178
                     /locus_tag="VBC23_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6842347.1"
                     /translation="MNPFKNFETRQVLEETCEVHGCQLWLTKVPIKGRLEELKQCPEC
                     TKAAINIFENKLNSQSKINSKLADTYAVFERDSLVSDKLRGKSLENYEIKDDIDQQAI
                     NYAKRMEQFYRQDRTGNAIVTGPSGVGKSHLTYGLAKFMNEQFKAYESPKSVLFISLV
                     SLFTKIRESFKADNGYRQAEMIELLTSVDYLFLDDLGKESRKGDSQNNEWTHQILYEI
                     LDNRSNTIINTNLSSKEIKALYADNYGNGALSSRILEGVTGNSFAYPKDMEDRRY"
     gene            19178..19426
                     /locus_tag="VBC23_000374"
     CDS             19178..19426
                     /locus_tag="VBC23_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842348.1"
                     /translation="MSYKATFMLYGYKAVPIGLFDTKELAAQAIVDHIIENSGIPRPR
                     VVATMKIRSKEIRIDYGSKTCYYLITLINNKTEEKQHD"
     gene            19419..19913
                     /locus_tag="VBC23_000375"
     CDS             19419..19913
                     /locus_tag="VBC23_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6842349.1"
                     /translation="MTKTMTLEEKVEQWFIDRNLHEANPVKQFQKMMEETGELYSGIA
                     KDKSEIIYDSLGDMQVVLIGIEQQIKNGAQIEASPQDMELLLLASSLGEMAQKLYKHI
                     FHNETKTPLIRPELSLLHSTIHAIAIHNLTTADDCLSIAYEEIKDRKGKLINGIWVKE
                     EDLK"
     gene            19910..20239
                     /locus_tag="VBC23_000376"
     CDS             19910..20239
                     /locus_tag="VBC23_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEO6842350.1"
                     /translation="MKNMNWKAFFNNLKAFASVIIITVFVLGFNYLFYEIGYQKGHQE
                     ADRVIIYVADNAGAEMFGKITDKEIIEGRHTVTAGAYGKFLVTEEQYNEITVGDDIPD
                     YLKGRGN"
     gene            20239..20658
                     /locus_tag="VBC23_000377"
     CDS             20239..20658
                     /locus_tag="VBC23_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO6842351.1"
                     /translation="MTKCNFEAGHKPIDTIGQLAKEVLDGKYGDGEERRRNLGCLYDL
                     VQTRVNYMVHERDSKFTGIKIYDNVNKPSHYQGKNGMEAIDVVRNFIGQLTGVSAFYW
                     GNSMKYLLRFQGKNGLEDLKKARKNLDWLIEEMEKEK"
     gene            20655..20861
                     /locus_tag="VBC23_000378"
     CDS             20655..20861
                     /locus_tag="VBC23_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842352.1"
                     /translation="MKKSIGILEFKEFDAFVGGKVMPVYLHSANPNVPTTNKNEAMRV
                     TKKRYRKEFSDKYIFRKVGEVDDQ"
     gene            20851..21267
                     /gene="ssb"
                     /locus_tag="VBC23_000379"
     CDS             20851..21267
                     /gene="ssb"
                     /locus_tag="VBC23_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6842353.1"
                     /translation="MINNVVLIGRLTRDVELRYTPSNIANATFNLAVNRNFKNAAGDR
                     EADFINCVMWRQQAENLANWTKKGMLIGITGRIQTRSYENQQGQRIYVTEVVADSFQI
                     LEKRDNSTNQASMDDQLPPSFGNSQPMDISDDDLPF"
     gene            21277..21537
                     /locus_tag="VBC23_000380"
     CDS             21277..21537
                     /locus_tag="VBC23_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842354.1"
                     /translation="MNEYKKPTYIVIQEAMAERIRFLEDELYNRAYKDIERLEVENDR
                     LKVRCLDLQLENADYVWDDMCRSAIARAKSENRRPHRRWRGR"
     gene            21534..21881
                     /locus_tag="VBC23_000381"
     CDS             21534..21881
                     /locus_tag="VBC23_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842355.1"
                     /translation="MKLRLEELRNKLGLSRRDVHEATGISMNTLLHYEKGGTPSIGQV
                     ENIAKAYNINPAWLLGWIDEGQQPQPLETVVEKIIYVEKEGCRLPPYWNNDNNGRIIK
                     WKESRRAFQRRTI"
     gene            21878..22342
                     /locus_tag="VBC23_000382"
     CDS             21878..22342
                     /locus_tag="VBC23_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842356.1"
                     /translation="MTKKTASKTRRDFLEFELEAKYLKIDKLIGQRRHELERIYAVKN
                     LTMPGIDDSGASRSGTSTNTSENLAVAYASDPMILKLEEFQNAISELLEVLEPDDKKI
                     FHLRWGEHTGYDWIQVWHIMENGETGYLYRHSKQIYRRREVILDTLAKLLFM"
     gene            22452..22994
                     /locus_tag="VBC23_000383"
     CDS             22452..22994
                     /locus_tag="VBC23_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6842357.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            23284..23571
                     /locus_tag="VBC23_000384"
     CDS             23284..23571
                     /locus_tag="VBC23_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842358.1"
                     /translation="MKDENAFDGIRLEAGSTLEIYLTKNDLEHIANGYEVTLDIKPNE
                     TVNKIVIKPSFVNNILNPLINYDKRIVSEADLKFRDISREVARDSFALGSM"
     gene            23571..23930
                     /locus_tag="VBC23_000385"
     CDS             23571..23930
                     /locus_tag="VBC23_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6842359.1"
                     /translation="MGYRNPKHSDWFRAWQIKFYNSKPWRTLRNKIRKAKRMRCDMCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEENCQLLCLECHNTKTFQSKINLNLDNRNI
                     NLFLIFLFLNFCGLPPF"
     gene            24018..24503
                     /locus_tag="VBC23_000386"
     CDS             24018..24503
                     /locus_tag="VBC23_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842360.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTRQERVELSRKTFKLIPKEKMLEISDNDSVVLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            24496..26208
                     /locus_tag="VBC23_000387"
     CDS             24496..26208
                     /locus_tag="VBC23_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6842361.1"
                     /translation="MVEMRYFDKYAQLIYTGKIRICKLTMKSIRRVERYKEQYLFKQE
                     EADKRIEFIEEECSNTKGLAGKLRLALPQKVWLETTWGFYHTVEVTKTNPDTLEEYTD
                     YEERRLIHEVPIIVPRGTGKTTLGSAIAEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDDKIIND
                     SVMPWIYQLDDVSEIHDPDMWQKAMPLLGITTEKETIARDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWSDKFDESLFVGDDERNARCVIGIDLSDVNDICSISFMV
                     VRGEERHYLNKKFMPRHTIETLPKELRDKYTEWELSGMLHVHELDYNDQAYIFEELRQ
                     FMSDNRILPVAVGYDRYNARELIRLFNDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNIFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            26217..27359
                     /locus_tag="VBC23_000388"
     CDS             26217..27359
                     /locus_tag="VBC23_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6842362.1"
                     /translation="MNNYLKNLKEVFARIFRPNNRKSTRTYLQRSISYWRRNSIYLDN
                     IYNKISTDTAQLRFKHVKITRNPGGVDSMVWYEHSDLAEVLTVSPNPLEVPVVFWSNV
                     TRAMLRDGVAVVVPRWKNGRLVEIWLAKKTVTWTAESVELMLDDVAVELPLTDVWVFE
                     NPKLNVTAQLNQITELIDINLNALTEKLSDGNSSLRGFLKLPTKAADEHLKQQARDRV
                     DSMLDLAKNGGIAYLEQGEEFQELSKDYSTASKEELEFLKSQLYNAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVYSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIGAEESE"
     gene            27409..27951
                     /locus_tag="VBC23_000389"
     CDS             27409..27951
                     /locus_tag="VBC23_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEO6842363.1"
                     /translation="MEKLKTFVVKSVEDESADFHFEAYASTYDNTDREGDVMAKGCFD
                     NTLKSKAVVPMCLNHDRNCVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFIKDYEPIDAKQPYGGWIFKEVEIFEISVVTVPANPQATVDNIKEFDMSAV
                     DKRIAQVNMRQEIMSKLAKI"
     gene            27962..29224
                     /locus_tag="VBC23_000390"
     CDS             27962..29224
                     /locus_tag="VBC23_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6842364.1"
                     /translation="MKKSLIELLEARQKATDELAEVKLKKATIEAKMKSSTIEDDDLE
                     QLKTDAESLVSQATAIKETIAGLDSDIEETEEELSKAAKIIKEKQKGNTPMDYLKTKA
                     AALDFVRILMDNEGSANSARKAWEANLVEKGVTNLTKILPEPVLIAIQDAFTNYNGIL
                     NHVSKDPRYAVRVALQTQVSQAKGHKAGKTKKDEDFTFLDFTINSATVYIKYAFEYSD
                     LKKDTTGAYFNYVMKELAQGFIRTIERAVVIGDGKSNSAEDKITEIKSIAEETEANLF
                     ETQEINVAGVFDNAVLETLVAGIDKMVPNTTPILVTSKAIARKLKLVKDAEGRYIDPQ
                     PFAPIATNGNVIAGFQVYIYDWMDGATNPIIAFADQAYKMIGDEAAADRFDDYDVTVN
                     RRHIELASVMGGRLAQYKSAVKFTNQAG"
     gene            29245..29580
                     /locus_tag="VBC23_000391"
     CDS             29245..29580
                     /locus_tag="VBC23_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842365.1"
                     /translation="MTILKTIKEMVEVDVEEDIFDVQLLRYINSGISYLQRNAIPVIR
                     IDKETELEGWTDIKEEDRETVLDWLHLRCVQRFDKSLMTGSAATMEWIDGEMTNLLYQ
                     LKAIYEVKT"
     gene            29577..29882
                     /locus_tag="VBC23_000392"
     CDS             29577..29882
                     /locus_tag="VBC23_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO6842366.1"
                     /translation="MKSSRVAIILCYDERVEVEKGVWEKKTVEKKVKAKKEKIYQRRL
                     DKAMADGQVLTARFSVRSNYVTDTLDYVKYNGKEYKVNIGTESDDGHYTIIELGELK"
     gene            29882..30229
                     /locus_tag="VBC23_000393"
     CDS             29882..30229
                     /locus_tag="VBC23_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842367.1"
                     /translation="MAKKFFTRQDIQSILETNPLKAKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDRVHMRKVTLQVSHYHKKKLDSIENLMLSNFMCEPNQLNLKQPDTDYLL
                     TTYRLEVFTNGRW"
     gene            30216..30560
                     /locus_tag="VBC23_000394"
     CDS             30216..30560
                     /locus_tag="VBC23_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6842368.1"
                     /translation="MGGGSVKMTPLKVDIKNQVLENIKKAAQSTENDIRTKSPRRNGV
                     YEKGWTHDIIDDAAVVHNNGKEKSLSHLLENGHATKNGGFVAPREHIRPAYIKNKEKF
                     LNDMKSIKITPK"
     gene            30575..31258
                     /locus_tag="VBC23_000395"
     CDS             30575..31258
                     /locus_tag="VBC23_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6842369.1"
                     /translation="MTYQYDTREVTHGNANGFYAKIAKTDAGALDLQKPYPFTGLRST
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTNSTPPALI
                     DTGVNTNFIWGYAETVTDEFGAEIEEFHIWTNVKASAPKGSTSTDETSATPKEIEIPC
                     TASPNNFIVDSEKKPVSEIVWRDDSKGTVRGKFDKLFADKSPGKLIDFINEALGTTTT
                     PTTTVSGGS"
     gene            31258..31734
                     /locus_tag="VBC23_000396"
     CDS             31258..31734
                     /locus_tag="VBC23_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842370.1"
                     /translation="MIKKELSFTAFDSYGEEREHTETVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGNLSDLTDEEKIKMMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDKKKPFQKL"
     gene            31920..34655
                     /locus_tag="VBC23_000397"
     CDS             31920..34655
                     /locus_tag="VBC23_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6842371.1"
                     /translation="MTETFEGLYVKFGANTVEFDRSVKGINTALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLVNLQEQARVGAMKIAELKKQQKALGESEVGSAQWNKLQLEIAKV
                     ESQMKIVDKAMESTKKHIEDVGDPKSILNLNKELDNVAKELDIVNQKLELDTDNVELA
                     EQKMKLLGKQSELVGDKVQELKKKQAALGDEKIGTEEWRQLQNEIGQAEVEVLKIDRA
                     MDILGESSRSATGDIKEATSYLRADVMMDVADKAGQIGQKMVDAGKMTVDAWSEIDEA
                     LDTVTTKTGLTGDALAELQEIAKDIATGMPTSFQNAGDAVGELNTQFGLTGEKLKSAS
                     ELLIKYAEINETDISSSAISAKQAIEAYGLTAEDLGMVLDNVTKAAQDTGQSVDTIVQ
                     KAIDGAPQIKGLGLSFEEGAALIGKFEKSGVDSSAALSSLSKAAVIYAKDGKTLTDGL
                     NETVSAIQNSTSETEALSIASEIFGSKAAPRMVDAIQRGAFSFDDLAEAAKSSSGTVS
                     TTFDETLDPIDKLTQYSNQAKEGMAELGGKLLETVIPALEPLMGMLESSVNWFTSLNE
                     TDQQTIVILGLVTTAVMMLLGAIAPLVIAIGAIGAPVGIVVAAIVGAIAVITLIIQAI
                     MNWGAITEWLQSTWDSCAAWLSELWTNIVTTATTAWSNFTAWLSGLWSSVVSTGQSLW
                     SSFTSSLSNIFSSLITGAQSLWSSFTSTLSNLWSGLVSTGSNLFNNLSSTISGIFNGI
                     LSTASNIWNSIKSTISNAIDGAKNAVSNGVNAIKNLFNFQIKWPHIPLPHFRVSGSAN
                     PLDWLKGGLPSIGIDWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAI
                     GQSIANTMNTSNNINVNFSGVTIREEADLNRLANVVGNRIAEELQRKTNLRGGMA"
     gene            34652..35374
                     /locus_tag="VBC23_000398"
     CDS             34652..35374
                     /locus_tag="VBC23_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6842372.1"
                     /translation="MTKINELTIDGVKTSSFKCDVLVETRPNVIVSSSKTALLEHDGI
                     SGAVVQSNRHRGLIEKPYHITLIEPSDEEIYRFSALLNREKFWLENEQEPTIRLWCYK
                     VDSFEIGKDEFGAWVVDVTFICHPTKFFKTTDTQTLTGNGVLRVQGSALAFPKITVVG
                     QSASETSFTVGNQVIKLEKLSESLVMTNDSDNPSFKTASGKLIKWAGDFITVDTAKGQ
                     NVGVVLGAGITSLKFETVWGWA"
     gene            35375..39049
                     /locus_tag="VBC23_000399"
     CDS             35375..39049
                     /locus_tag="VBC23_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6842373.1"
                     /translation="MLFLLDKDVRTVKWNGIPLHEASSAIVKEETNSDFYLTVRYPIT
                     DSGIYQLIKEDMLIKAPTPVLGAQLFRIKKPIENDDSLDITAYHVSDDIMKRSITPVS
                     VVGQGCAMALSQMVQNAKTGLGDFSFTSDIMDSRTFNTTETKTLYSVLMDGKHSIVGT
                     WEGELVRDNFALSIKRSRGADRGVVITTHKNLKSYQRTKNSQGVVTRIHARSTFKPDG
                     AEDEVTLRVSVDSPLINSYPYINEKEYENNNAETVEDLRKWAEAKFTNEGIDKVSDAI
                     EIEAYELDGQVVNLGDTVNLKSRKHSADLYKKAIAYEFNALTEEYISITFDDKPGVGG
                     SGVSSGLSNVADAILVASATAQDVAVQRAVKNANAAFDAEFGKTKTKINDDIEIAKAK
                     VESFKSELSNRMDNQLLPLATEAKNLASQAQADLTRKEIELRAELNRQVTSTETVKIS
                     LTNLSHNMDIIKQKALNDLRDAETRLKEADSVQQLATKRVEDKLTGLSTKLESFSVGG
                     YNYVIDGGEPKELMANFYGKTYDINPQLLERTSQATLSFSYEAESTSRLEVRLYKKMH
                     TGDTSKITIIVMPNFDLSPGKGFISQSFDLGGVMPDPRNQAWLVMRGTNANPLTLSKV
                     KLERGTVATDWNNRDETLKASFAEYKQTVDENLANLRTSTETLAGQLTSAESSIRQTS
                     ESFSNRLVSLETYKDSESNRASRYFEASKSETAKQLSALRTEVNGSYVDKSTYEENAR
                     GLTRRFESLSSGTQNYALGTERDVYLNVSGTTVYDLYSFGTTLAIKGIEMGDTVTIAF
                     NYFADSRTAFGSYELELYGHAGKIERVGEVQRTISNRGTYTASFVANNANFRANSLKI
                     RFTDSRLKFRVTTLRVTKGTIPADWSPSPDDLKAYSDTKLEQTANEIKASVTSLDHKT
                     LKQTDITMTSEGIVLRAGKTSNDVARAIGSYFKVTPDAIALFSSLIKVSGNMLVDGSV
                     TSRNLVTGAVETGHVKAGAITGVLLAAEAVTAEKLKVDQAFFNKLMANDAYLKQLFAK
                     SAFITQVQSVTISASQISGGVIKALNNAMEIQMNSGQILYYTDQAALKRVLSGYPTQF
                     VKFATGTVSGKGNAGVTVIGSNRYGTESTNDGGFVGVRAWNGSNIDSLDLVGDKIRLA
                     SSAFDNSDGWDVRTLDSGLKITPHNRAAERNSRIEVGDVWILKGNGSYSSLRDILNSF
                     NENFSKGPNADSYTYYHSGF"
     gene            39066..39296
                     /locus_tag="VBC23_000400"
     CDS             39066..39296
                     /locus_tag="VBC23_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842374.1"
                     /translation="MNQEQQLNQALRLTVNELTAQLANESTTKNLLAIQLTEVDQEKQ
                     QLTQQNAELQARVSELEGLLDEQTQPEIIEGE"
     gene            39299..39637
                     /locus_tag="VBC23_000401"
     CDS             39299..39637
                     /locus_tag="VBC23_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842375.1"
                     /translation="MTTTENNLLDLETITEPFDLATALKYMKENGELIRCKNAVNDFY
                     MYRDMQKRPVIVNGRRQFKDVETVWAFNQWGGTTPTINMADLFNLEYYIMTFDENGNP
                     DWTEPHLEDK"
     gene            39672..40082
                     /locus_tag="VBC23_000402"
     CDS             39672..40082
                     /locus_tag="VBC23_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842376.1"
                     /translation="MPPWLIDSTVVVAMVTVLGGLFSTIITTSANRKDQLIKHQYEDI
                     KEDLSGLIDKVKTIDHTTTETKKISEITKDGTLKIQRYRLFHDLTKEISQGYTTIEHF
                     RELSILFESYQLLGGNGEIEALFEKFKQLPIEED"
     gene            40085..40414
                     /locus_tag="VBC23_000403"
     CDS             40085..40414
                     /locus_tag="VBC23_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6842377.1"
                     /translation="MTQITDIIISSAMGILTILAGIAVQAVKVYLIKKGGEKAVLITE
                     ILAKNAVNAVEQVATETGFKGADKLTSAKAQILAELQKYNIHMSDDDLTLFVESSVKQ
                     MHDAWKE"
     gene            40418..41824
                     /locus_tag="VBC23_000404"
     CDS             40418..41824
                     /locus_tag="VBC23_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6842378.1"
                     /translation="MEINTETAIAWMSARQGKVSYSMDYRDGPNSYDCSSSVYYALRS
                     AGASSAGWAVNTEYMHDWLIKNGYELIAENVDWNAVRGDIAIWGMRGHSSGAGGHVVM
                     FIDPENIIHCNWANNGITVNNYNQTAAASGWMYCYVYRLKSGASTQGKSLDTLVKETL
                     AGNYGNGEARKAVLGNQYEAVMAVINGKTTTNQKTVDQLAQEVIAGKHGNGEARKKSL
                     GSQYDAVQKRVTELLKKQPSEPSKAQEVNKPTETKTSQTELTGQATATKEEGDLSFNG
                     TILKKAVLDKILGNCKKHDILPSYALTILHYEGLWGTSAVGKADNNWGGMTWTGQGNR
                     PSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAIKGM
                     FKVGGAVYDYAASGFDSYIVGASSRLKAIEAENGSLDKFDKTTDIGDGSKDKIDITIE
                     GIEVTINGITYELTKKPV"
     gene            42031..42216
                     /locus_tag="VBC23_000405"
     CDS             42031..42216
                     /locus_tag="VBC23_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO6842379.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            42277..42681
                     /locus_tag="VBC23_000406"
     CDS             42277..42681
                     /locus_tag="VBC23_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO6842380.1"
                     /translation="MKYTYLALFEVDKENGGYNISFPDFPGAFSEADSLNEAIFNARE
                     VLEIYTIMFEDEGKEFPKASSFKALASNLASDEDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(42895..42965)
                     /locus_tag="VBC23_000407"
     tRNA            complement(42895..42965)
                     /locus_tag="VBC23_000407"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42931..42933),aa:Cys,seq:gca)
     gene            complement(43099..44631)
                     /gene="ahpF"
                     /locus_tag="VBC23_000408"
     CDS             complement(43099..44631)
                     /gene="ahpF"
                     /locus_tag="VBC23_000408"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6842381.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(44649..45209)
                     /gene="ahpC"
                     /locus_tag="VBC23_000409"
     CDS             complement(44649..45209)
                     /gene="ahpC"
                     /locus_tag="VBC23_000409"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6842382.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            45486..46256
                     /gene="rpsB"
                     /locus_tag="VBC23_000410"
     CDS             45486..46256
                     /gene="rpsB"
                     /locus_tag="VBC23_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6842383.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            46350..47390
                     /locus_tag="VBC23_000411"
     CDS             46350..47390
                     /locus_tag="VBC23_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6842384.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(47549..48010)
                     /locus_tag="VBC23_000412"
     CDS             complement(47549..48010)
                     /locus_tag="VBC23_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6842385.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            48183..48647
                     /locus_tag="VBC23_000413"
     CDS             48183..48647
                     /locus_tag="VBC23_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6842386.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            48644..51091
                     /locus_tag="VBC23_000414"
     CDS             48644..51091
                     /locus_tag="VBC23_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6842387.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQCIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
BASE COUNT        16332 a         9047 c        10745 g        14987 t
ORIGIN      
        1 aaacaacggt ttcttttttc ttgaatcaaa aatttgaaaa aatgtcggat gagacgaggc
       61 agcgtattca agaggtgatt gatgaaacag gttatagacc aagtacgata gcaagaagtc
      121 taaattctaa aaaaacaaaa ctactaggtg tcttaattgg agatataaca aacacatttt
      181 caaatcaaat tgtaaaagga attgagcaca tcaccaaaca aaaaggctat caaattattg
      241 tgggtaatag taattatgat gccaaaagtg aagaagatta tattgaaaat atgttaaatt
      301 taggtgttga tggtttcatt attcagccta catcaaattt tcgaaagtat tctcgtattt
      361 taaaagaaaa aaagaaacca atggtatttt ttgatagtca actttatgaa cataagacga
      421 gttgggttaa ggctaataac tatgatgctg tttatgatat gacacaggag tgtctaaatc
      481 gagggtataa aaagtttatt atgattactg ctgatacgag cttgcttagt acacgtattg
      541 agagagcttc gggattcatg gatgctttaa aggacaatgg tttcggttat gacactttgg
      601 tgattgaaga tgatgatcat tcaaaaagtg atatagaaga ctttctaaaa gcggtagttc
      661 cagataagga ggagacatta gtatttgcac caaattgttg ggctttacca atggttttta
      721 ctgccatgaa aaatctcaat tttgatatgc cacgagttgg tttagttggg tttgacaata
      781 ttgaatggac agacttttca tctcctaaag tgtcaacaat tgtccagccg gcttatgaag
      841 aaggtgaaca agtagcacag attttgatta atcgtattga aggagatgac tccgtcgata
      901 accaacagat tgtagattgc cagatgtttt ggaaagagtc gactttttaa aaaaagattt
      961 cttattttat ttgctattaa aaaataaaac ctagagaaaa ttatctctag gttatttgtc
     1021 cttattctaa aataagtaag ccttctttga cagcgaaagg atttttctca ttgatacggt
     1081 cgtaaaacat aataccatca atgtggtcaa tttcatgttg aacaacaata gagttgtaac
     1141 cttttaattt gagtcgatgt ttttcaccag ttttgtcgaa atattcaata gttacacgcg
     1201 cgtggcggac gacatagcca ggtacttcac gatctactga taggcatccc tccccgtcag
     1261 ataaagcagc atcttgaaca gaatgcgata caactttagg gttgtacatt acttcttgga
     1321 ggctataagc ttctttaggt ggatttcctt gagcatcctc aacgtttgga acaaggacgg
     1381 caatgatgcg ttttgagatg tctaattgtg gtgcggctaa accaacacca ccacgtaagc
     1441 ctagcttctc agccataatt ggatcttgag agtgttttaa aaattgcatc attttttctc
     1501 cgagaatttc ttctttctca gaaagtggga atgtgacttc ttcagccact ttgcgaaggg
     1561 ttggattgcc ttcgcggata atgtcattca tgtcaattaa atgactagct tttactaatt
     1621 tatcaatagc agacatattt tctcctaact gtattctctg atttagttta gcataaaata
     1681 attaaatgat aaagctacgc ttcatggctg aatatttaat gattctagtg cgaaacgatt
     1741 tttgataaag tattttggtt tttctttttg gataaggcct tctttgacca tatcatggat
     1801 aactcttaat aaatgacgat aactgatgcc aaacgaatca gctaaactac ttagttcaag
     1861 gtgaaagcaa ccttgttctt caattgctaa aatatgactt gcaagtaact ctttgacggt
     1921 ataccctaaa ttagtagacg ctttgatgtt ttgatcatga aaagcctgag ctagctcttg
     1981 actaagtttt aggaggaaag ttgcgtctgt taacagtttc tctttccgtc ctttaagtga
     2041 taattgaatg actgtaatat cctctaaggc aataacagat gatacaattt gtctattagt
     2101 caaaagttca atatcaccaa tcagtgttgg ttttgtttta atatctaaaa tatgttcttt
     2161 gccgttaaag agtcgacgta caattttaaa acgtcccttg acgatgtagt gcaagtattc
     2221 taattgttct ccctgtttac agacggattg gccggcagtt agggtgatta cttgtaaatc
     2281 cttgaaataa tgttttagaa agatagtctc caaacagtag tcttggcagt agttttctat
     2341 atccatgtat tgactcccta tttgagatag ttttaggaca tatgtcatat atttctagtt
     2401 taatattgat tatactttat ttatctaaaa aaagacatca gaaagaggta ttgtcacttg
     2461 aaaatcttta tggagaagct aagtttactc tctttatcac ttatcttgct atcaactttt
     2521 tcgacatcac cagccctacc acaaatgata tcttattata gagataaggg cttaccatca
     2581 ccccaagttg aattattatt ttcaattcct tcgatggcta ttatcttcat cttgcttatc
     2641 acaccttggt taagtaaaaa actttctgag aaacatatga ttatctttgg actattgcta
     2701 acagcattag ggggaggatt gccggttgtc agtcagaact accttttagt atttgtatcg
     2761 cgtcttttat tggggagtgg tatcggtttt attaatgcta gggccattag tgtgattagt
     2821 gaatattatc aaggcaaaga aagaagaaaa ctcttaggat taagggggtc ctttgaagtt
     2881 ttaggaaatg ctggtttgac agccttagtt gggctattgt tgacttttgg ttggtctaaa
     2941 tcatttatga tttatttttt agctttacca atcttagttc tatatcttgt ttttgcgcct
     3001 aagaaagtgg tgaaagatac aaatgacaag attaaaacta aaggtcaaaa aatccctaaa
     3061 gctgatttga cttatattgt cgccttagct attttagcag gatttgtcat aactataaat
     3121 actgggatta atcttaggat acctctgcta gtagtagaat ttggtttggg tacgccagct
     3181 caagctagtc ttgttcttag tgcgatgatg cttatgggaa taatagcagg tatgagtttt
     3241 ggtcagttga tagctatgtt tcataaacaa ttaattccaa tctgcctcgt cttattttct
     3301 ttaacattgc ttggagttgg attgccttct aatctaatgg tactaacaat tagtgcgatg
     3361 gcgtcaggtt tcctttacag cctgatggtc actgctgtgt ttagcctagt agcagaccgt
     3421 gttgaatatt cgttggttgg ttctgcaacg acattagttt tagtattttg taatattggt
     3481 ggagctagtg cggctattct tctgtcttgt tttgatcatt tactaggaca aataaatgca
     3541 gttttctatg tttatgctat tttaagttta gcagttggta tgatttattt ccttaaacgc
     3601 ctatttttta agaaaatgta gtgtatatta actaaaaagg gcttgacaat cattgtcaag
     3661 ctctcttcat ttttcttctt ctgggtaggt aaatccccat tcttgacgta gctgtgtcat
     3721 gatatccata acatctttgg tatagtttaa gtacatgtgg ttagtttttc ctgaaatggc
     3781 ttcttccata tcagctacct catattggag agcattttca gttttgccag cttcgataat
     3841 atcttgatgc ccatcctcag tataagtaat aactgccttt tgtcctcgcg gatattcaaa
     3901 aagttcaatg tagcctttat cgtaagcgat agttgctcgt ttaggttgtt ttgcatgtaa
     3961 actaaggctg acagtcgcca tctcatttgc tgggttggtt agtaggatac caacttgttc
     4021 atcaacccct gttggtgcaa atgtaacttg agaggtaatg ttgtgaggtg cttctgacat
     4081 aaaccagcga atacaagaaa gtgcataaac accaatgtcc agcaaagcac cgcctgctag
     4141 gtcacgactg aaaaaacggt tagtcatatc atattcttta taacttccga aattcatttg
     4201 aatcattttt aacggtccta attttccact atcaactaat gtttttaatt ggcggtaaat
     4261 tggcatatga aaaatagtca tggcttcagc taagacaaca tggttagttt cggctaaatc
     4321 tatggcttct ttaagctcag tactatttaa agtaatagat ttttcgcaaa gaacgtgctt
     4381 accatttgct aaagcctttc gtaaaaatga gatgtgagta ttgtggggag tagagatata
     4441 aatgatatcc acttcagggt cttcaaatac ttgatctatg tgatcataaa ctttttggat
     4501 accatatttg ttagcaaatt caagtccttt gtcgtaagtt ctattagcta cagaatataa
     4561 tttttgtcca cgtgcttcca gtgcttgggc taattcgttg gcaataacgc cggttccaag
     4621 cgttgcccaa ttataatatt tttcagtcat aacaacctcc ttgttggttt atagtatagc
     4681 aaaaataaca aaatgatatt taaagaaagc accaatatga gagtattttt aatagtcaaa
     4741 ctctgaattt tctgttagac tgtaagagat tacatatgag atattaagga gaaaataatg
     4801 ataaaatatc aagatgattt ttaccaagcg gttaatgggg aatgggctaa gacagctgtt
     4861 attcctgatg acaaaccaag gacaggtggt ttttctgatc ttgctgatga cattgaagca
     4921 ttaatgctgt caacgacaga taagtggctg gctgatgaaa ataaaccaag tgatacgatt
     4981 ttaaatcatt ttattgcttt tcataaaatg acagctgatt atcaaaaacg tgaagaggta
     5041 ggagtatcac cagtattacc attaattgaa gaatataaag gtctacaatc tttctctgag
     5101 tttgcatcaa aagttgcaga atatgaacta gaaggtaaac caaatgaatt tccatttggt
     5161 gtggctcctg actttatgaa tgctcaattg aatgtacttt gggctgaagc gcctgggatt
     5221 attttaccgg atacaactta ttatagtgaa gataatgaaa aaggaaagga actccttgct
     5281 ctttggcgta aaagtcaaga agacttactg cctttgtttg gcctttcaga acaggaaatt
     5341 aaagatatct tggataaggt tttagctttg gatgcaaaat tggcacaata tgtcttatct
     5401 cgcgaagaga gttcggaata tgttaaactt taccatcctt ataactggga agatttcaca
     5461 aaattagcac ctgaacttcc attagatgct attttccaaa agatattagg acaaaaacca
     5521 gataaagtta ttgttcctga agaacgtttc tggacagaat ttgcatctga ctattactca
     5581 gaatctaatt gggaattatt aaaggcagat cttattttat cagctgcgaa tgcctataat
     5641 gcttacttaa ctgatgatat tcgtatcaaa tcgggagttt acagtcgagc tttatcaggc
     5701 acaccacaag caatggataa gaaaaaagca gcttactacc ttgcttcagg accttacaat
     5761 caagctctag gtttgtggta tgcaggggaa aaattctctc cagaagctaa agctgatgtt
     5821 gagcataaga ttgcaacgat gattgatgtc tataagtcac gcttagaaaa ggcggattgg
     5881 cttgcacagt caacacggga aaaagctatt gtgaaactta atgttatcac gccacacatt
     5941 ggataccctg agaaactacc agagacttat actaaaaaaa tcattgatcc taaactatcg
     6001 cttgtcgaaa atgctactaa tttagacaaa atttcaatag cctatggttg gagtaaatgg
     6061 aataaaccag ttgatagaag tgaatggcat atgcctgccc acatggtcaa tgcgtattat
     6121 gatccacaac aaaaccaaat tgtcttccca gcagccattt tacaagcacc attttatgct
     6181 ttagagcaaa gttcatcagc aaattatggg ggaattggtg cagtgattgc acatgaaata
     6241 tcacacgctt ttgacactaa tggagcttca tttgatgaac atggaagttt gaataattgg
     6301 tggactgatg aagattttaa agctttcaaa aaacttacag ataaagtagt tgagcagttt
     6361 gatggactgg aatcttatgg cgcaaaagtc aatggtaaac taacagtatc agaaaatgtt
     6421 gcagatttag gtggagttgc ttgtgcttta gaagctgcgc agcgtgagtc agatttttca
     6481 gcgagggatt tctttattaa ctttgcgaca atttggcgta tgaaggcacg tgatgagtat
     6541 atgcaaatgt tagctagtgt agatgttcac gccccagcac aatggcgaac taatattaca
     6601 gtaacgaatt ttgaagaatt ccataaagag tttgatgtta aagatggtga taacatgtgg
     6661 cgtccagttg aaaaacgtgt tattatttgg taactctaaa tgataaactc ccgttactca
     6721 gatgagtagc aggagtattt ttttattctt ttatagataa agataatgta gtagtgtcaa
     6781 cgcttatctc agctccaata gtcactgtca aaagaggttg ggtgtgcgca aaatccatat
     6841 cataaataac aggaatttcc ttgaaaatag catgttttga aaggatatat tcaaagactt
     6901 gtggagtcat gccgcattct ttaggaaagc gccccataag aatagcttgt ggatgaggat
     6961 aagcttgtaa aattgcagca aggttgcggt caaattcata aaaattactt tcctcagcct
     7021 cttcaatcaa gaggacatag cgttcaattt tcggtgcata aggggtacct cgaagtaggc
     7081 caaacgtaga gagattacct ccgataatcg taccggaagc ttttccgtga ttataaattt
     7141 tccattctgt tgggaaaaat tggcgtggtt tgctgggatc ataccaggga tcactagacc
     7201 attcttcact aggccaaagt tcatagtggt tttctgtcat ggcagtaagc caagcttgtg
     7261 tttggtaagg ttgaccttcc ttcattttga aagaagaata agcaggaccc atgtaagttt
     7321 ggatttttgc tttggcaaaa atagcattaa ggaaggcggt tgaatctgag taaccacaaa
     7381 ttattttggg attcttagag attaaatcat aatctaaata tggtaaaagc tcgttggaat
     7441 tgaatccgcc aatggttgct aatatggcat caacagaagg gtcggcaaat gcttcatgta
     7501 aatcttctac gcggctagca atcgaagcag aatagaggat atcattttct agatagtgct
     7561 tagagaatga cacttgaaat cccagctttt ctagtacttc tttggctaat aggttagcct
     7621 caaaaccacc cagacgttca atggaagaag agggactaag aacacgaatg tggctgcctt
     7681 ttgttaattt tttcattggt taacctccta tttaaattgt tgttagtcta ccacatatgg
     7741 agataatgag caacgatatt cttaaattct atcaatttaa gaaatgcgtg ttatactaga
     7801 gatagccgat gaaagaaaaa atacttatta ttaataattt ggttattaaa taggaggtca
     7861 tgatgtctca attatctttg gaagaacgat tgaatcgact aaaagataaa tacctttctt
     7921 ctaaaaatca agaattttca aatgttcact taagtaaagt aaagaagaaa aagaagaaat
     7981 taataatact tgaaaaagaa agatgtcgct gtcgattgga gagagaagat actaaaaaaa
     8041 tcgatgataa aattgagaaa ttgaagaaag aattgataga tgttatgaag gaggaataat
     8101 atggaagaaa ctattctaat agtcagcttt ctcctgtttt tgattttatc taatgttatc
     8161 aataggatat ttcctaaatt accattacca tttatccagt tggtctttgg gattctctct
     8221 ggcttagttt ttcataagtc acaagtccat atcgatcctg agttatttct tgcctttgtt
     8281 attgctcctt tgaactttag ggaaggtcaa gaaagtgata ttggtagttt tgtaaagtat
     8341 cggactatta ttctttactt aatcttacca acggtatttt taacagctat tgtcgttggt
     8401 tatgtagcag ggcacctttt gccagttagt ttaccacttg cggcatgctt tgcgcttgga
     8461 gcagccttag gcccaacaga tgccgttgcc tttatatcga ttgctaagag gtttcaattt
     8521 cctaaacgag cagagaatat cctcaaattg gaaggtcttt taaatgacgc tagtggtcta
     8581 gtttccttcc agtttgcttt aacagccctt gtaacaggtt atttctcctt agcaaaagct
     8641 agtctcaagt tagcattggc cataatgggt ggttttttaa ttggcttgct ttttgctttt
     8701 ctaatgaggt tatgtctcac tgtcctagag aaatttgatg ctgctgatgt tacaggggct
     8761 ctacttttgg aattaacctt accttttgtt gcttatttcg ttgctgattt gttgggattt
     8821 tcagctatta ttgcggtggt agtggcagga gtaatgcaag caaatcgttt gaaaaaagtg
     8881 actctctttg atgctcaagt agatcgcgtt acctctgtta tttgggaaac cttaaatttt
     8941 attttaaatg gtttagtttt cttaatattt ggaagagaac tgatacgtat cattggtcct
     9001 cttttaacaa gtaacgctta tagtaatttt gatcttatta gtattgttgt tttagttact
     9061 tgcacacttt ttctagttcg ctttctagca gtttctcgct tttatgcttg gagatctttc
     9121 aaataccata aatcatttaa aaagtattgg agagagattc aactgttgac tttttcagga
     9181 gttaaaggaa gtgtttccat agcaacaata ctcttattac ccaagcactc agtcatagga
     9241 gagttggggt atagtttgat tcttttcact gttggtgcag taacgttaat gagtttttta
     9301 acgggacttt tagtattacc aaagttagcg cctcccttac aggtgaaaga tgattattta
     9361 atacgtctat cgattttaac aaaagtatta tctgtcttag aagaagacgg taagagttct
     9421 gaaaatcaag cttcatttta tgcagttata gataattata atagccgtat tcgtcatctc
     9481 attttagaac aagaatctag tgacattaag aaggacttag ctgaattgca actgatgatg
     9541 cttagtatag aaagcgacgg gcttgaagct gcttatcgtt atggcaatat ttctatcaaa
     9601 gaatatcgta tttaccagcg ttatttaaaa tacttagaac gtcaagttaa tcgtagcttt
     9661 gtgtcaaata tcacttatag tttaacgatt cttatgcgtg ttattcgtgg tattgttcat
     9721 gagtttttaa catttggaca aggaatacga aatcggaaaa aacatttcaa aaacaggaaa
     9781 cggttgttag cagaagtcaa tcgtcagcac ttaacagatt tgtacttatc aaataatgac
     9841 ctcattttag aaacgctaga agatatggaa ggttattata atgcaacttt gatagatttc
     9901 ttaaaaaatg aacgcattcg agacgcagaa ttaattaaat caggtttatt tgtagaacgt
     9961 gtcattgcac gcataacgcc agatatttta aatgaaaccc taagaggata ttacttggaa
    10021 cgcagaacaa tctctgaatt tgaaagggac ggtttaatta gttctaaaga gtctcaaatt
    10081 ttaagggata atgtcaatga attagaaagc tattcattaa gagatagtca atcttctata
    10141 ccttatcaat tattggattt aaaaaaataa ttctgataac aaaaaaatca acgaacaatc
    10201 gttgattttt ttgttatctt tggataaaat cttttatatt taaggcggta gacggatttg
    10261 aaaaccttgg tttatcaagg tttttggttg tttcgcccca aattcgcccc aaattatgcg
    10321 actaaaaaaa tatttttgat tttctcaaaa tttctctcag ccagtgcttc catctgatgt
    10381 gtataaactt tcagagtaat atctggactt tcatgtccaa ggagcttaga aattgtcaca
    10441 atgtcaaccc cgttcaaaat caaaaacgaa gcgtatgtgt gccgtaatga gtgatttctg
    10501 actttcctac ccacaatttt cttaatcagt ttattgcaag ctgaattaga tacaccaaag
    10561 cacactctat tcttgatatt agcttgccaa tgatttttct tatattctcg aagaatatct
    10621 attgtatttg aatcaattgg aacttttctt tttgatgact cattcttcag atctgcaaaa
    10681 tcttgagtat tcgagtaatc aaaactcttg tttatatcca aaatgccatt tttgaagtct
    10741 atatcgctcc aggttagtcc catgacctct gagaaccgca aaccagtgac agaaagaagg
    10801 tagagagtga aataggatac atactggata ttctctcttg tgagggataa aagagcctta
    10861 tactcacttt cttctaagaa gtcattttcc tcagctcttg attcaatttg tgatttaacc
    10921 tttgcatcat cagcaaagtt aaaatgaata acttgttccc tcacagctac tttcatagct
    10981 cccttgattt ggtagtggaa cttctcaaga gtttcttgag catatttctc cccaaattca
    11041 tttagccgtt tttgataata gagtggtgtt atatccttaa cctttatatc tttaaaatag
    11101 gttttgatgt gtctaaagtt ctttgtgtag gtttcccagg tcttatcctt tacataagga
    11161 cgtttataaa cttctgacca agttttgaca aaatcatata gagtaacttc tccatcggtc
    11221 aggatatttt gagatagttt tttctcaata tccctagcag ctgcttgagc aagttttttg
    11281 gttttgaaac cactcttaga cttttgacgg aacttaccgc tctcatcttt atatgaaatg
    11341 cgatattccc agccattatc acgttttcga taataagcca tttgtttttc acctcatttt
    11401 ttgataaaat gggtatagta aagagaccta ctgctaaagc aggtttctca ctatccacaa
    11461 ttgccttacg cttgcaccga ccaaagttga gcgtagggct ttttttattt tttaacttta
    11521 tttttcaacg cattctcgat agcattttta agctcaagaa tagctatttt ctcatcttta
    11581 ctgaaggtta ctgtattttc gtcttttact gcatcgaaaa caccgccctt agtatcagat
    11641 gaaccaggat aaaccaattg tagatagcca acagttgaac taggttcctt gagttgataa
    11701 gctgtaattt ctgataatag aattgacttt tctccatcta agccatgtag caacatattt
    11761 gacatgttgc tttttcttgc gattctgatg aagtaatcat caatacgtac aattgttttt
    11821 gattttttga attcaaaaac acgttcgtta ggctctgatg taaagagttc tacttcaatt
    11881 tctttatctt ttccgccaaa taatgccatt attgttgtct ccttttgttt ttattgtaac
    11941 cgtaaaatgt taaaaatctg ccctccacct tgacggtagt ttaaaactaa cttgagggcg
    12001 tagctttcag catactgcaa gttatagatg agatagttca tcagtctgtt gtgtagtgct
    12061 ggtttgctaa tatctgtttc acgttgaata tcttcaaagc tctctctagt tgtgagggct
    12121 tttttgagtc gttcatctgt taggtagagg attgaagcca ctgtatcagc ttctttctca
    12181 attggaatca gctctttcgg gtacttctca ctagcgtttt tactcatgag agacatatag
    12241 attggctgat tttctgtttg attaaggtga cagtgtatgt gactaagttc gtgtaaaatc
    12301 gtgaagataa cacgtccttt ggtgtttgta tgttgattga tatagatgat gtagcgtcca
    12361 aggtctaggt cagggacagt caatccagca cagctttcgc agagtaccct atcagtataa
    12421 gtaacggttc tatttgatac cagccctctg tacttgatgt ctttatctgt gggatgataa
    12481 tcaggtaatt ctggataacg ttctttcatc tcatcgtaat caagaaagtt aaagagaata
    12541 ggatagtttt gttcaaaatg tttaatgaca tcttgataac taacgttctc tttcatttta
    12601 tcaattgagc ttattatttg ataagcctta ttgtgatagt caaaatactg ttcccttgat
    12661 agcggtgtgt atttcaataa gtcacctact tccagttaga atcatcttga atcaattgtc
    12721 tagctgtctt cattagacct tcaagagcag aattgaaccg ctctttttca gaagctgaaa
    12781 gtccttctgt ttccttacga aaaagaacaa tagcttgctg agttagatca tcaactgcag
    12841 tattatcttg gccatcggca atcttaggat tttctgttct accgagaagg tagtcagtgc
    12901 tgacattgaa ataatctgct atttttgaga cacgctcaac atttggtgta gattttttca
    12961 tgttgtatat ggtatttcta ggaaaatcca actgttcttc aagttgattg agtgaaatac
    13021 cttgcttgtc agccaattcc ttgatctttt caaatgtaaa aaacattgat ttatcaacct
    13081 ttctaaggca tgacaaaaaa tatttaataa atttactaca aaactgttga caggttttaa
    13141 taaatttact acaatatcgt ttgtaagcta agtcgttagc gaacaagaca agtaaaaaat
    13201 aaagcctaaa caactgattg gcgtcggttt tatgggttaa gccttagctt tttagcaggt
    13261 cttttctcta tgctttgatt ttaataaatt tatttattgt tgtcaagaag ttcgctaact
    13321 ttttagcaaa aataataaaa ggagggtaga tgaatgggaa tcggttcaat gacagtgaca
    13381 gttaaagtta ctaacatgga taaatttatt gaacttagta aagagttcaa taaaaaagct
    13441 cgcgagctag aagaactagc tcacgaactg aagtcatttg attttgaagg cgaagttgta
    13501 tcaactgata gcaactagct caaatgattc atcatctgaa aaaagataaa cagggtattc
    13561 ttctttagtt gcaaaattga agaaatcata tccaaaatta tcttttttga tgtgggaatt
    13621 tttttcggag agagagtcaa gattgacctg agatagtagc ttaaagattt gcacacgagc
    13681 ttgttcagca tcgttaactc tttgcaaggc tctcaagtgc tcttcgctag ggaaagctgc
    13741 tatcaaatca gctctgtcat ctatgtaagt tctagtgtca tcaagatagc catattttag
    13801 attatagtct acaaatgacc gctgttcata ttcttgatag gtttcaaagc tgacaaggtt
    13861 acctttgtaa cgatagacga gaacgtcatc ttgtaactct tcttcgaaat ttgtgatgcc
    13921 aattagaaat ttatggcatg cctctatgat acgaatttga ttcaaattca tggcttgacc
    13981 tcctttcata aaagatttca agtcaattat accaaactag aaaggaaaaa tatgagtcaa
    14041 caacagaaaa aatggattca gctagtgaaa gataagctga atgaagagca aatgacacag
    14101 acacatctag cccgtgcttg tggagtcgca aaagcgacaa tctcagagct actaaaatac
    14161 ggaaaaggta gtgtccgtct gaaaaataaa gtctcagaca ttcttcacat tgatgaaagc
    14221 tggacggact tggaggaaga ttgatgccga taacaagaga aatgacaatc actgaaatca
    14281 atgttctcaa tgctatcaag aatagcgcta cttatgatct tccgattcaa gccagagagc
    14341 taaggcagca gctaggactt agcaaacgat cacttgaagc agtcattgag aacctgagag
    14401 tgatttacaa gcaaccaatc gttgccaaaa aaaaacagcc tagcggatac tatcttccaa
    14461 gaaatgaaga agaaagaaac gacggcttag ccccatacaa aagacagata ttgactgagc
    14521 aaaataatct tgctgctatt atgtctgtca atctggaaga atattgggca acagaaaaag
    14581 cctgacggca atcaggctca caaaatatta ttacaagagg attatatcac aatgaacgaa
    14641 gtttggaaag atataccaat tgttaaagga tattatcaag tttccaatct tggtcgtgtt
    14701 cggtctattg gtagaacagt caatgcaaaa cagagaacaa gaaaaacaaa aggacgcatc
    14761 ttaaaacaga gcttatcttc aggctatgca atagtgacac tttcagtcaa tggattaagg
    14821 aagtcgataa gagttcatag actagtagct gaagcattta taccaaatcc aatcaataag
    14881 cgaacgataa atcatattga tgaaaataaa cttaataata gagtagataa tttagaatgg
    14941 gcgacagata aggaaaatgc taatcatggg aatagaacaa cgaaatcatc tttaggtcgt
    15001 tgtaaacctg tagaacaatt tacacttgaa ggggaattca taaacacgtt tgactcaata
    15061 aaatctgcat caatgaaaac aggtatctcg tcacaaagaa taacagcaac agcaatgggg
    15121 caccaaaaac agactcacgg atataaatgg aggtacgcat aatgaatgat ctaatgattc
    15181 aaatgcttga ccagttcgaa gctggtctaa tggacagaac gatgaaagta atgcacgttg
    15241 tgacagacga aaagtataga tttcctttag aactcaacaa gtctcaatgc gctttaatgc
    15301 tcttaggtac taaagatact gggacatttg atgaacgctt taactgtcat aaggattttc
    15361 cgaggattcc aaatgctcgt gagaaatacc ctcgtgatga agtcattgaa tggtatcaca
    15421 agaattggca aagaactgtt atctaagaaa gaacaaaagg aactatatca tgaacaattt
    15481 acaaattatc gcagtaggca cacttatttc agtaaccctg attgagtctc ttatctcaaa
    15541 tatcaagctc agaaaagctc tcaagaacaa acagacagct gggactgtca ttgctcaata
    15601 tatcccacaa cgaggcttca ttgactacaa gactggaaga cgtgttgaca ttgaccctga
    15661 aacaaagaaa gaagtctttg ttgactgagg aaagctatgg atgaattaaa agtattgcct
    15721 cacgatatcc aggctgagca gtcggttctt ggttcaattt ttatcaaacc tgaaaaaatg
    15781 attgaggtgg ctgagtatct gaaaccaaat gacttttaca ggccagctca caagattcta
    15841 tttaaggcca tggtgagcct tgctgatcgt ggcgaggcca tcgatattgt cacaatcaag
    15901 tcaacgctgg aaagtacgga tgaacttggc atggttggcg gtatcagcta catcgctgag
    15961 attgttaatg cagtgccaac cagctcacac gctgagcatt atgccaagat tgttgctaaa
    16021 aaagcacagc tacggtctat cattgacaac ttgtctgact caattggcaa tgcctatgat
    16081 gaggacatgg atattgatga gattatcgca aaagctgagc gatcacttat cgaagttagt
    16141 caagccagta acaagagtag tttcagacct atccatgatg tccttttgga aaatcattca
    16201 aagattgagg aacgctcaaa caataccagt caaatcactg gtattgaaac aggattttat
    16261 gactttgaca agctgataac aggcttgcat gaagaccagc taattgtctt agcggcacgg
    16321 ccagcgatgg ggaagacagc cctagctctt aacatagctc agaatgtggc aactaagtcc
    16381 aataaggctg tggccgtctt ctcgcttgaa atgggggctg aaagccttgt tgagcggatg
    16441 ttgtcagctg aaggaacaat catcaaccat catattagaa caggaaattt gacggttaat
    16501 gagtggcaac gtcttatcta tgcacagggt cagcttgctg aagctccaat ctttatagat
    16561 gacacagctg gagtcaagat tactgacatc agagcaagag ctaggaggct atctcaagag
    16621 actgacggac ttggattgat agtcattgac tatcttcaac tcattcaagg gtcacggtct
    16681 gacaaccgac agcaagaggt atctgagatt tctaggcagt taaagattat tgccaaggaa
    16741 ttaaaagtgc cagtcattgc actatcacag cttagccgtg gggttgagca aagaaacgac
    16801 aaaaggccta tcatgtccga cttaagggaa tcaggcagca ttgagcaaga cgctgatatt
    16861 gtcgccttcc tctatcgtga tgcttattac caagacaaaa aagaaggtca gccagaaaac
    16921 gacatcactg agctgatcat cagaaagaac agacacggaa accttggcac agtcaagctg
    16981 tatttccaca aggaatatac caagttttct agcgttgagg aggaatgaaa tgaaaaaatt
    17041 tgaccactat ttcagcaacc atattgctgt aaaaaacgaa tctgatacag tcaccattgt
    17101 tgactattac aatccagaaa agggctatga gtataactta cgttacattt tcgatagaaa
    17161 aaattcatcc cttgctatca caggagactt tggggaatta gttgcacgaa atttctacaa
    17221 tatgggtgaa tgggacacat tctacaatca ctatactggt catctggggt attttcttga
    17281 aaagttacta tgttcaagta ggccagttca ttactatgat actgacaagg ctaaaagaga
    17341 aattctcaat gaattctttg atggtgctga ttatgatgat ttagattttg atgatgaatt
    17401 cctacttgat caattatttg acaattttga cgaagataaa ggatttactt atattcttcc
    17461 agaggctttt gaacgtttta gcgaagatat ggaagaatat gaggtctatg aatttctatc
    17521 aagggctggc cgatacgtca atgatgtctt tgaactttac ttagacgctt ataagagagc
    17581 ttatgagtat ctaacaaagg aggttgatga tggcacaacg cagaatgttt agtagaaaaa
    17641 tcactgagac tgaccgtttt ttggaaatgc cactatcatc ccaagctctc tatttccatt
    17701 tgaacatggg agctgatgat gagggcttta tagataaggc aaagacgatt cagagaacta
    17761 ttggcgctag tgatgatgat atgaaattac tgattgcaaa agggttcttg attccctttg
    17821 atagtggtgt agtcgttatc cgtcattggc ggattcacaa ctatatccag tctgaccgct
    17881 ttcaatcaac cttataccag tcagaaaagg ctcagctaga gtatgacaag tcaaaaacat
    17941 ctagtcttaa acctattgga aattgtatac aaaatgtatc caaaatggag acacaggtta
    18001 ggttaagtaa gggtagctta gataaggata gcttaactac ctatcctaca gtttcagaca
    18061 atgaggaaga agatattcca tacaaggaaa ttatttctta cttgaatgaa aaagctaata
    18121 gaaattacag acctaacatt cagaaaaaca aaactcttat caaggctaga tggtcagaag
    18181 gattcaggtt agatgatttc aaacatgtga ttgataccac ggtcaaggat tggtcaggca
    18241 ctaagtatga aaagtatctc agacctgaaa cgctctttgg ttctaagttt gaaggctatt
    18301 tgaatcaagc accacgaatc aagacggaaa caattgacgg aaggttgggc ttttagatga
    18361 atccttttaa gaattttgaa actaggcaag tcttggaaga aacctgcgag gttcacggtt
    18421 gccagctctg gctgactaaa gtaccgatta agggacggct tgaagaactc aagcagtgtc
    18481 cagagtgtac caaggccgct atcaacatct ttgaaaataa gttgaacagt cagagcaaaa
    18541 tcaacagcaa gcttgctgat acatatgctg tctttgaaag agatagcttg gtatcagaca
    18601 agctaagagg caagagcctt gagaactatg aaatcaagga tgatattgac cagcaagcta
    18661 tcaattatgc taagcgtatg gaacagttct accgacagga tagaacaggc aatgccattg
    18721 taactggccc ttctggtgtt ggaaaaagtc atctgactta cggcttggcc aagtttatga
    18781 acgaacaatt caaagcctac gaatcaccaa aatcagtcct cttcatctca ctggtgagcc
    18841 tttttaccaa aatcagagaa agcttcaaag ctgacaatgg gtaccgacag gctgagatga
    18901 ttgagctgct aactagtgtg gactacctct ttctggacga tctaggcaag gagagtcgca
    18961 aaggcgacag ccagaacaat gagtggactc atcagattct ctatgagatt ttggacaatc
    19021 ggagtaacac aatcatcaac acgaatctga gcagtaagga gattaaggcc ttatatgctg
    19081 acaattacgg taatggtgct ctttctagtc ggattcttga gggagtgact ggcaatagct
    19141 ttgcttaccc taaggatatg gaagatagga ggtattgatg agctataaag ccacattcat
    19201 gctatatggt tacaaagctg ttccgattgg tctatttgat acaaaggagt tggccgctca
    19261 agcaattgtt gaccacatca ttgaaaactc tggaatacct agacccaggg tagttgccac
    19321 tatgaaaatc cgaagcaagg aaatcagaat tgattatggt tctaaaactt gctactactt
    19381 gatcacttta attaataata aaacggagga aaaacaacat gactaaaaca atgacacttg
    19441 aagaaaaggt tgaacagtgg tttattgacc gtaacttaca tgaggccaat cctgttaagc
    19501 agtttcagaa gatgatggag gaaactgggg agctttactc tggtattgct aaggacaaga
    19561 gtgaaattat ctatgattcg cttggagata tgcaagttgt cctgattggc attgaacaac
    19621 aaatcaagaa tggcgctcag attgaagcaa gtccacaaga tatggagttg ttgctattgg
    19681 cttcaagttt gggggaaatg gcacagaaac tctacaagca tatcttccac aatgaaacaa
    19741 aaacaccgct tatccgtcca gaattaagct tgcttcactc tactattcat gcaatagcta
    19801 tccacaactt gacaactgct gatgactgcc tatcaatcgc ttatgaagaa atcaaagaca
    19861 gaaaaggcaa gttgatcaat ggcatttggg ttaaggagga ggatttgaaa tgaaaaacat
    19921 gaattggaaa gcattcttca acaatctcaa ggcctttgca agtgtcatca tcattactgt
    19981 atttgttctt ggattcaact atctgtttta tgagattggc taccagaaag gccatcagga
    20041 agctgacagg gtgattatct atgtcgctga taatgctggc gctgagatgt ttggcaaaat
    20101 cactgataaa gaaatcattg aaggccgtca cacggttaca gctggcgctt atggaaaatt
    20161 tctggtcact gaggaacaat acaatgaaat caccgttggt gatgacatac cagactacct
    20221 gaaaggacgt ggaaactaat gacaaaatgt aattttgaag caggacacaa accaattgac
    20281 acaattggac aactagccaa agaagtcctt gatgggaaat atggcgatgg tgaggaacga
    20341 agaagaaatc taggctgttt atacgatcta gttcaaacaa gagttaatta tatggttcat
    20401 gaacgtgaca gcaagttcac aggtatcaag atttacgaca atgtgaacaa acctagccat
    20461 tatcaaggaa agaatggcat ggaagccatc gatgttgtca gaaatttcat tggacagctc
    20521 acaggagtat ctgctttcta ctggggaaac tcaatgaagt acctgttacg cttccagggc
    20581 aagaacggcc ttgaagacct caaaaaagca agaaagaacc ttgactggtt gattgaagaa
    20641 atggagaaag agaaatgaaa aaatcaatag gaatccttga atttaaagaa tttgatgctt
    20701 ttgttggtgg aaaggttatg cctgtctatc ttcatagtgc taacccaaac gtgccaacga
    20761 ctaataaaaa tgaggctatg agggttacca aaaagagata cagaaaagag ttctctgaca
    20821 agtacatttt cagaaaggtt ggtgaggttg atgatcaata atgttgtact tattggccgc
    20881 ttgacaagag atgttgagct acgttacacg ccgtcaaaca tcgcaaacgc tacttttaac
    20941 ctagcagtca atcgaaattt caagaatgct gctggtgatc gtgaagctga tttcatcaac
    21001 tgtgtgatgt ggcgacaaca agctgaaaac ttggccaatt ggacgaaaaa aggaatgctg
    21061 attggtatta ctggacgaat ccagacaaga agctacgaaa atcagcaagg tcagcgtatc
    21121 tatgtgactg aggttgttgc tgacagcttc cagattcttg aaaagcgtga taattcaacg
    21181 aaccaggcaa gcatggatga ccaattgcca ccatcatttg gaaatagtca gcctatggat
    21241 atttcagatg atgatctacc gttttaggag gtgcttatga acgaatataa gaaacccacc
    21301 tacatcgtca ttcaagaggc aatggctgag cgtattagat ttcttgaaga tgaattatat
    21361 aacagagcat acaaagacat tgaacggcta gaagttgaga atgaccgtct gaaggttaga
    21421 tgccttgatt tgcagttgga aaatgctgac tatgtctggg atgacatgtg tcgctctgct
    21481 attgctcgtg caaaatcaga aaatcgaaga ccacacagaa gatggagggg aagatgaagt
    21541 taaggcttga agaactaaga aacaaacttg gcctcagtcg cagagatgtg catgaagcca
    21601 ctggcatttc tatgaacacg ctgctacatt atgaaaaagg cggtacacca tctattggtc
    21661 aggttgaaaa tatcgctaag gcttacaata tcaacccagc ctggttgctt ggttggattg
    21721 atgaaggcca gcaaccacaa ccacttgaaa ccgttgttga aaagattatc tatgtggaaa
    21781 aggaaggctg tagattaccg ccatattgga acaatgataa taatggcaga atcatcaagt
    21841 ggaaagaatc aagaagagca ttccaaagga gaacaatttg acaaagaaaa cagcaagtaa
    21901 gacaagacgt gatttccttg agtttgagct tgaggctaag tatctaaaaa ttgataaact
    21961 tattgggcag cgccgtcatg aactggaaag aatctatgca gtcaaaaacc tgacaatgcc
    22021 aggaatcgat gattcaggag ctagtcgcag tggaacttca actaatactt ctgaaaatct
    22081 agctgttgct tatgccagtg acccaatgat tctgaaactg gaagagttcc aaaatgcgat
    22141 atcagaatta ctagaggtac ttgaacccga tgataagaaa attttccatc tgaggtgggg
    22201 agaacatact ggatatgatt ggattcaagt ttggcacatc atggaaaatg gcgagacagg
    22261 ttatctttac agacatagca agcagattta tagaagacgt gaagtcatcc ttgatacact
    22321 tgcaaaatta ttgttcatgt aacttgtcaa aaaaacatat agaattgaca gaaacaatct
    22381 gatagattga tagtgtcgct aagcaccgag aaatccttgg tgctttattt ttttgagaaa
    22441 ggagcaaagc gatgaatatt gttgaaccgc tacgagacaa agacgatata caagccatga
    22501 aggactatct atcatcttgg aatgaaaagt attacatgct atttcttttg ggcatcaata
    22561 caggtttccg tgttggagat attctcaagc taaaggtcaa agatgtccaa ggctggcaca
    22621 tcaaagttag ggaacaaaag actggtaagt ataaaagtat taagatgaca aggccactca
    22681 aaaacgaatt gagggaattt gtcaaagaca aggaacttca tgagtatcta tttcagagtc
    22741 gtgtcggaaa gaacaaggcg ctcagttata agacggttta ctggtttctt aaaagagctg
    22801 ctgaagacct cggcattgac aatgttggaa ctcatacaat gcgaaaaacc tttggctatc
    22861 attactacaa gaagtacaag aacgttgctg acttgatgtc actattcaat cattcaagcc
    22921 cagcagttac actaatctac atttgtgtaa ggcaagatga gcttgatact aagatgagta
    22981 attttagcct ctaatatttt tttgattttt tcaactatcc ataacgagga agtttctagt
    23041 ctatattttg aagtgagctt aaagccttgt cctaattagt tttctagtgt gaaacaaaat
    23101 tggataaaat ataagatata gatagttcag catggttatt ttacataatt tcagccatag
    23161 aaaaataatc ttgtcaaaaa aatgggtatt attgacaaaa acaatctgat atatttgtaa
    23221 catgagaaaa atccagagag caagcttagg catgttcttt tttttgttgg gggtgacgat
    23281 gccatgaaag atgaaaatgc atttgatgga attaggcttg aagctggtag cactttagaa
    23341 atctatctca cgaagaacga cttagaacat attgcaaatg gatatgaggt gactttagat
    23401 attaagccta acgagactgt aaataaaatc gtaataaagc caagttttgt aaataatatt
    23461 ctaaatccgt taatcaacta tgacaaaaga atagtgagtg aagcagattt aaagtttaga
    23521 gacatttcaa gagaggttgc aagagatagt tttgcattgg ggtcaatgta gtgggctata
    23581 gaaaccctaa acactctgac tggttcagag cttggcagat taaattctac aactcgaaac
    23641 cttggagaac tctgagaaat aaaatcagaa aagctaagcg tatgcgttgt gacatgtgcg
    23701 gacgtttgat tcacggtaag agtattgttg accacatcat agagattgac gaaactaatt
    23761 atcaagatga atctatcact ctcaacgaag agaactgtca gttactttgt cttgaatgtc
    23821 acaacacaaa aacttttcaa agtaaaataa atttaaattt agataatcgg aatataaatt
    23881 tatttttgat ttttttattt ttgaattttt gtggactccc cccattttga attttgacag
    23941 cgccaaaata ataacggtgt caatcctctt gtgtacctct cccccaaaat tgacgaaaat
    24001 tgatacaaga aaggagcatg attttgaaaa tcaatgaagt tttagaaaag ctaggaataa
    24061 gtcgtgctac ccttaccagg tatcgaaaaa agctgggcat atttgaagaa actaggtcaa
    24121 atatcaccaa aagtcagttc aaagagttgg aaaagcttgc caatcaacgg caaaagtaca
    24181 caaggcagga acgtgttgag ctatctcgta agactttcaa gctgattcca aaagaaaaaa
    24241 tgcttgaaat cagtgataat gattcagttg tgttgaaaaa tctcaaaact caatacaatc
    24301 acaatcaaaa agtgattgaa aacttccagc ttgaaatcaa taaggtcatc aatgacggtg
    24361 agctacctga taagtaccta cttgatggaa tggaaaagta tcaaaagctt aacatgcaga
    24421 tcatgtcaac gattgaaaag caaagtccac agggtgacag tctcaaagaa atgattcagg
    24481 agaagttagc acgttatggt tgagatgaga tattttgata agtatgctca gctcatctat
    24541 actggtaaga ttcgtatttg taagctcaca atgaaatcaa ttagacgtgt tgagcgatac
    24601 aaagagcaat acctcttcaa acaggaggaa gctgacaaac ggattgagtt cattgaggaa
    24661 gagtgcagca atactaaagg ccttgctggt aagttacgct tagcattacc acaaaaggtt
    24721 tggttagaaa caacgtgggg cttttatcac acggttgagg ttactaagac caatcctgat
    24781 accttggaag aatacacaga ttatgaagaa aggcgtctca ttcatgaggt gcctattatt
    24841 gtgcctcgtg gcacaggtaa gactactctt ggttctgcta ttgctgaggt tggtcaaatc
    24901 attgacggtg agtggggtgc tgatattcag cttcttgctt acagtcgtga acaggctggc
    24961 tatttgttca atgcctcaag ggcgatgttg tcgaatgaag aaagcttgct gcactatatg
    25021 cgtgaggctg acatcctacg gtcaaccaag caaggtatct tgtatgaaac aactaacagt
    25081 cttatgtcta tcaagacttc tgactatgaa agccttgacg gtactaatgc tcactacaat
    25141 atctttgatg aggtgcacac ttatgatgat gacttcatca aggttgtgaa tgatggttcc
    25201 agccgtaagc gtaagaattg gataacctgg tacatttcca caaatggaac taagcgtgac
    25261 aagctctttg ataagtatta caacatctgg gtagatatcc ttgatgacaa gattatcaat
    25321 gattctgtca tgccttggat ttatcagttg gacgatgtgt cagagattca tgaccctgat
    25381 atgtggcaga aagctatgcc attacttggt atcacgacag agaaagaaac catcgctcgt
    25441 gatattgaga tgagcaagaa tgatccagca caacaagctg agctgatggc taagactttc
    25501 aatcttcctg tcaacaacta tcttgcttac ttcagcaatg aagagtgtaa aggttggtca
    25561 gataagtttg atgagagttt gtttgtcgga gatgatgaac ggaacgcccg ttgtgtgatt
    25621 gggattgact tgtcagatgt caatgacatc tgctctatct cttttatggt tgtgcgtggg
    25681 gaagaacggc actatctaaa caagaaattc atgccacggc ataccattga gacattgcca
    25741 aaggaactgc gtgataagta cacggagtgg gaattaagtg gcatgctgca tgtgcatgaa
    25801 ttggactaca atgaccaagc ctatatcttt gaagagttac ggcagtttat gagtgacaac
    25861 agaattttgc ctgtggcagt cggttatgac cgctacaatg caagggaact tattcgcttg
    25921 tttaacgact actacgggga tatttgtcac gatattcccc agacggtcaa atcgttatca
    25981 aatccgctca aggtttacaa ggagaaggct aagatgggca aaatcatctt tgatgatcct
    26041 gtggcgacat ggaatcatgc caacgtccgt gtcaaaattg atgccaataa caatattttt
    26101 ccaaacaagg aaaaggcaaa agaaaagatt gacgtctttg ctagtcagct agatgccttt
    26161 atctgttatg aaaatttcaa ggaagacttg agctactact ttgattgagg tgaagaatga
    26221 acaactattt gaaaaatttg aaggaggttt ttgctaggat tttccgtcca aacaatcgga
    26281 aatctacaag aacctatctt caaagaagta tctcctactg gcgtaggaac tccatctatt
    26341 tggataacat ctacaataag atttcaactg acacagctca gttaaggttt aagcatgtca
    26401 agattactcg taacccaggc ggtgtcgatt cgatggtttg gtatgagcat agtgatttgg
    26461 ctgaggtgct aacagtttca ccaaatccac tagaagtgcc tgttgtcttt tggtcaaatg
    26521 taactagggc catgctgcgt gacggtgtgg cggttgttgt accacgttgg aagaatggcc
    26581 gactggttga aatatggctt gctaagaaga cagtgacttg gacagcagag agcgtggagc
    26641 ttatgcttga tgatgttgct gttgagcttc ctcttactga tgtgtgggta tttgaaaatc
    26701 ctaagttgaa tgtcacggct caactcaatc agattactga gcttattgac attaacttga
    26761 atgctttgac agaaaagctt agcgatggca attctagctt gagaggtttc ttaaagctac
    26821 caactaaggc agctgatgaa catttgaagc aacaagccag ggaccgtgtt gatagtatgc
    26881 ttgacttggc caagaacggt ggcattgctt atcttgagca aggcgaagaa ttccaggaac
    26941 ttagcaaaga ctattctact gcttctaagg aagaattaga gttcttgaaa tcacagcttt
    27001 ataatgctca tggtatcaat gagaaattat ttacctgtga ctatactgag gaacaatata
    27061 gagcctacta ttctagcgtc atgaagttat atcagcgtgt ctattctgag gagattaaca
    27121 gaaaatactt cacgaagaca gcacggactc agggcaataa gttactggtc ttctttgata
    27181 tggctgacat gatttcattt aaggatttgg ttgaaggtgg cttcaagtcc aaatatgctg
    27241 gattgatgaa ctcaaatgag ttccgtgaaa cctatcttgg tttacctggc tatgagggcg
    27301 gtgaagtatt tgaaaccaac ctgaatgctg ttcgtatcgg tgcagaagaa tctgagtaga
    27361 atctaaaggg tgggtggttg gcatatcttt cacgaaagga ggtaagcaat ggaaaaacta
    27421 aaaacctttg ttgtgaagtc ggttgaggat gaatcagctg actttcattt tgaggcttac
    27481 gcttcaacct atgacaatac agacagagaa ggcgatgtga tggccaaggg gtgttttgat
    27541 aacactctga aatctaaggc tgttgtccct atgtgtctta atcatgaccg taactgtgtt
    27601 attggtaagc atgaattgtc tgtggatgaa aaaggcttgc gaacacgttc aacgtttaat
    27661 cttagtgatc cagaggctaa gaaaacttat gacctcatga aaatgggggc attggatagc
    27721 ctgagtattg ggttctttat taaagattat gagcctattg acgctaagca accttatggc
    27781 ggatggattt tcaaggaagt tgaaatcttt gaaatttctg ttgtgaccgt gccagctaac
    27841 cctcaagcaa ctgttgacaa tattaaggaa tttgatatgt cggcggttga taagcgaatc
    27901 gctcaggtga acatgaggca agaaatcatg agtaaacttg caaaaattta aaggagacat
    27961 gatgaaaaaa tcactcattg agcttttgga agctcgtcaa aaagcaactg atgaactggc
    28021 tgaggtaaag cttaaaaaag ccactattga agcaaagatg aaatcttcaa ccattgaaga
    28081 cgatgacttg gaacagttaa aaactgatgc agaaagtttg gtttctcaag caacagctat
    28141 taaggaaaca attgctgggc tggattctga cattgaagaa actgaagaag aactcagcaa
    28201 agctgctaaa atcattaaag aaaaacagaa aggtaataca cctatggatt atttgaaaac
    28261 caaagccgct gctcttgact ttgtccgtat tctgatggac aacgaaggta gcgcaaacag
    28321 tgcccgtaaa gcgtgggaag ctaaccttgt tgaaaaagga gtgactaacc tcactaagat
    28381 tcttccagaa cctgttctta tcgctattca agatgccttt actaactata atgggatctt
    28441 gaaccacgtt tcaaaagatc cacgttatgc ggtgcgtgtt gctcttcaaa ctcaagtatc
    28501 tcaagctaaa ggacacaaag ctggtaagac taagaaagat gaagacttca cattcttgga
    28561 tttcactatc aactctgcga cagtttatat caaatatgcc tttgaatatt ctgacttgaa
    28621 gaaagataca acaggcgctt acttcaacta tgtgatgaaa gaacttgctc aaggcttcat
    28681 ccgaactatt gaacgtgctg ttgtcattgg tgatggaaaa tctaacagtg ctgaagacaa
    28741 aatcactgaa atcaaatcaa ttgctgaaga aactgaagcg aatctctttg aaacacaaga
    28801 aatcaatgtt gctggtgtat ttgacaatgc tgttcttgaa acgctggttg ctggtattga
    28861 taagatggtt ccaaatacta ctccaattct tgtcacttca aaagccattg ctcgtaaatt
    28921 gaaattggtc aaagatgcag aaggtcgcta catcgatcct caaccatttg ctccaattgc
    28981 aaccaacgga aatgttattg ctggttttca agtttacatc tatgactgga tggacggtgc
    29041 aactaaccca atcattgcat ttgctgacca agcttataag atgattggtg atgaggctgc
    29101 cgctgaccgc tttgatgatt atgatgtaac agtcaaccgc cgtcatattg agcttgctag
    29161 tgttatgggt ggtcgtttgg ctcagtacaa atcagcggtt aagtttacaa accaagcagg
    29221 ttaaatatag aaaggggagt ctgaatgaca atccttaaaa caatcaaaga aatggtagaa
    29281 gttgacgttg aagaggatat ctttgacgtc caacttttac gatatatcaa cagtgggatt
    29341 tcatatttac aaagaaatgc tattcctgtt atccgtatcg ataaagaaac ggagttagag
    29401 ggatggacag acattaagga agaagatagg gagacagtgc ttgactggtt acatctgaga
    29461 tgcgtccaac gttttgacaa atctttgatg acaggcagtg ctgctactat ggaatggatt
    29521 gacggagaga tgacaaatct tttgtatcaa ctcaaggcaa tctatgaagt gaagacatga
    29581 aatcttcaag agtagctatc attctttgct atgatgagcg tgttgaggtt gaaaaaggtg
    29641 tctgggagaa aaagaccgta gaaaagaaag tcaaagctaa gaaagagaaa atctatcagc
    29701 gaaggcttga taaagctatg gcagacggtc aagttctgac tgctaggttt agtgttcgtt
    29761 ccaactatgt gactgacact cttgactatg tcaaatataa tggcaaagag tacaaggtta
    29821 acattgggac agaatcagat gatggtcatt acactatcat cgaacttgga gaactgaaat
    29881 aatggccaag aaatttttca ctagacaaga tattcaatct atccttgaaa caaacccttt
    29941 gaaggctaag gtcttctaca tggaacgtga ggagaaatct tctcctgaca atgtcatctt
    30001 gtattatcgg ttaacaccag gcagtagcat tactgctgat gatagagtgc acatgagaaa
    30061 agtgactttg caagttagtc actaccacaa gaagaaacta gacagcattg aaaatttgat
    30121 gctgtctaat tttatgtgtg agccaaatca attgaatcta aaacaacctg acactgacta
    30181 tctactgaca acttacagac ttgaggtgtt tacaaatggg cggtggtagc gtgaagatga
    30241 caccgctaaa ggtggatatc aagaatcaag ttttagagaa cattaagaag gctgcccaaa
    30301 gtacagaaaa tgacataaga accaaaagcc ctagacgaaa cggtgtttac gaaaaaggtt
    30361 ggacacacga catcatagat gacgccgctg ttgttcataa taatggcaaa gaaaaatctc
    30421 tatcccacct attagagaat ggtcatgcca ctaaaaacgg tggctttgtt gcccctagag
    30481 agcatatcag accagcctac atcaagaata aggagaaatt ccttaatgat atgaaatcca
    30541 ttaaaatcac acctaaataa gaaaggagtc ttacatgact tatcaatacg atacaaggga
    30601 agtcactcac ggtaatgcga atggttttta tgccaaaatt gctaaaactg atgctggtgc
    30661 cttagacttg cagaaacctt atccctttac agggctacga agcacatcct ttgaaacttc
    30721 tcaggaatca aatgcctact atgcagacaa tgtggaacat gttcgtttgc aaggtaaaaa
    30781 atccacagaa gggtctatta caacctatca gattcctaaa cagtttatga ttgatcactt
    30841 aggtaaaaag cttacgaact ctacaccacc agctttgatt gatactggtg tcaatactaa
    30901 cttcatttgg ggttatgctg aaactgttac agatgagttt ggagctgaga ttgaagagtt
    30961 ccacatttgg acgaacgtta aggcatctgc acctaaaggc agcacatcaa ctgatgaaac
    31021 atcagcgaca ccaaaagaaa ttgaaatccc ttgtactgcc tctccaaata acttcattgt
    31081 ggattctgaa aagaaacctg tatcagaaat tgtatggcgt gatgacagca aaggaaccgt
    31141 ccgtgggaag tttgacaagt tgtttgctga caagtcacca ggcaagctga ttgatttcat
    31201 caatgaagcg cttggaacaa ccactacacc taccacaact gttagcggag gctcttaatg
    31261 attaaaaaag aactatcatt cacagcgttt gatagttacg gtgaagaaag agagcacact
    31321 gaaacagtgc gctttcttta ctctttacca gctatcaaga tgtatgaaca gcgaactggt
    31381 cgtaacttct ttgatgataa ccaaaaagca cttacagcat atacacaact tgctcttgca
    31441 actggtgtaa acggtaacct atctgattta actgatgaag agaaaatcaa gatgatgcct
    31501 ctacttatgg aacctgattt catgaacttc ttgactgaag tcattccttg cctttatggt
    31561 gaggttgaaa atggacgttt agtacaaaat gagcttaccg ctgaaacagc ttcattagct
    31621 ccttggtttg gtgatttgat tgatattggt ttcttctcag acctcttcta tgaatttaac
    31681 cgaagcagag caaaggtgcc gcaagataaa aaaaagcctt ttcagaagtt gtaacttccg
    31741 aaaaggttta taaggttatc tttgaaaatc ggatggatgt tttttgggca gaatcccaac
    31801 actttaacta tttgatggga acacttcacc aaatgagcat caatcaggaa gagaagaaaa
    31861 ccttatccaa cgctgattta cttagcgtga tgtcagatta aaaagaaagg aggaaatcta
    31921 tgactgaaac gtttgaaggc ttatacgtca aatttggtgc taatactgtt gaatttgata
    31981 ggtctgtaaa aggtatcaac actgccttat ctagtttgaa aaaagacttc aataacatca
    32041 acagacaatt gaagatggat ccagacaatg ttgacttgtt gaatcgtaag ttggttaact
    32101 tgcaagaaca ggctcgtgtt ggtgctatga aaattgctga actcaaaaag caacagaagg
    32161 cactgggaga atctgaagtt gggtcagcac agtggaataa gcttcaactt gaaattgcta
    32221 aggttgaatc acagatgaag attgttgata aggcaatgga gtcaacaaag aaacacattg
    32281 aagatgtagg agacccaaag tctattctga atcttaacaa agaacttgat aatgttgcta
    32341 aagagcttga tattgtcaat caaaagcttg agctagacac tgacaatgtc gaactagcag
    32401 agcaaaaaat gaaactactt ggtaaacagt cggaattggt tggggataaa gtccaagaat
    32461 taaagaaaaa acaagctgcc cttggcgatg agaaaatagg tacagaagaa tggcgtcaac
    32521 ttcaaaatga aatcggtcaa gctgaagttg aagttctaaa gattgaccgt gcaatggaca
    32581 ttcttggtga gtcaagccgt tctgcaactg gagacatcaa agaggcaacc agctatttaa
    32641 gagctgatgt catgatggat gttgcagata aggctggtca gattggccag aaaatggttg
    32701 acgctgggaa aatgacagta gatgcttggt ctgagataga tgaggctctg gacaccgtca
    32761 caaccaaaac tggtctgact ggtgatgcct tagcagagct tcaggaaatt gctaaagaca
    32821 ttgctactgg tatgcctacc agctttcaga atgctggtga tgccgttggg gaattgaata
    32881 ctcagttcgg tttgactggg gaaaagctga aatcagcatc tgaattactt atcaagtatg
    32941 ctgagattaa cgaaacagac atttcaagct ctgccatttc tgcaaaacaa gctattgaag
    33001 cttacggttt gacagctgaa gacttgggaa tggtcttaga caatgtgacc aaagccgctc
    33061 aagatacagg acagtcagtt gacacgattg ttcaaaaagc cattgacggt gctcctcaga
    33121 ttaaaggttt gggactttct tttgaagaag gtgctgcact gatcggtaag tttgagaaaa
    33181 gcggtgtgga ttcatctgct gctctatcct ctctatcgaa agctgctgtc atctatgcta
    33241 aagacggtaa gactctgaca gatggattga atgagactgt tagtgctatt caaaattcta
    33301 ctagtgagac agaggcttta agtattgcct cagaaatctt tggtagtaag gctgctccta
    33361 gaatggtcga tgctattcag cgtggtgctt ttagcttcga tgacttagct gaagcagcta
    33421 aaagttcctc tggtactgtc tccaccacat ttgatgagac gcttgaccca atagataagt
    33481 tgactcagta ttctaaccaa gcaaaagagg gaatggcaga acttggcggt aaattgcttg
    33541 agactgtcat cccagcttta gaacctttga tgggtatgct tgaatcttct gtcaattggt
    33601 ttactagcct aaacgaaact gatcaacaga ctatcgtgat tcttggccta gttacaactg
    33661 ctgtgatgat gttgcttggt gcaattgcac cgctggtcat cgccataggg gcaataggtg
    33721 cgcctgtcgg aattgtagtg gcggcaatag taggggctat tgccgtcata acacttatca
    33781 tccaagcaat catgaactgg ggagccataa ctgaatggct tcagtcaacg tgggattctt
    33841 gtgctgcctg gctttctgaa ttgtggacta acatagtcac gactgccacc acagcgtggt
    33901 caaatttcac tgcctggctt tctggccttt ggtcttcagt agtctcaact ggacagtctt
    33961 tgtggtctag ctttactagt tccttgtcca atattttctc aagtttgatt acaggtgctc
    34021 agtctctgtg gtcaagtttc acttccactc tttccaattt gtggtctgga ctggtctcaa
    34081 ctgggtcaaa tttgtttaat aatttgagta gcacgatttc aggaattttt aatgggatac
    34141 tttcaacagc aagcaatatt tggaattcca taaaatccac tatttccaat gcaatagatg
    34201 gggcgaaaaa tgcagtgtcc aacggggtca atgccatcaa gaatctgttt aacttccaga
    34261 ttaaatggcc tcatattcca ctacctcact tccgtgtgag tggttctgct aaccctctgg
    34321 attggctaaa aggtggctta ccaagtatcg gcattgactg gtatgccaag ggcggtatca
    34381 tgaccaaacc aaccctattt ggcatgaatg gaaaccgtgc aatggttggc ggtgaggctg
    34441 gcgctgaagc catcttgcca ttgaataagt caaccctggg ggcaattggt caaagtattg
    34501 ctaacacgat gaatacatcg aacaatatta acgttaactt ctctggcgtc actatcaggg
    34561 aagaagctga ccttaacaga ctagccaacg tggttggaaa tcgtattgct gaagaattgc
    34621 aacgtaaaac taatttgaga ggaggaatgg catgacaaaa atcaatgaac ttaccattga
    34681 cggtgtgaaa acatcatctt ttaagtgtga tgtattggtt gaaactagac caaatgtcat
    34741 tgtctccagc tccaaaacag ctctattaga gcatgatggt attagtggtg cagttgtgca
    34801 atcgaataga caccgtgggc taattgagaa gccttatcat atcactttaa ttgagccaag
    34861 cgatgaagaa atttatcgct tttctgctct tttgaaccgt gaaaagtttt ggttggaaaa
    34921 tgaacaggaa ccaactatta ggctttggtg ttataaggtt gatagctttg agattggaaa
    34981 agacgaattt ggtgcctggg tggtcgatgt taccttcatc tgccacccta ccaagttttt
    35041 taaaacaact gacacccaga ctttgactgg gaatggggtt ttgagggtgc aagggtcagc
    35101 tcttgctttc ccgaagatta cagtggttgg tcaaagcgct tctgagacat cgtttacggt
    35161 ggggaatcaa gtgattaaac ttgaaaagct ctcagaatcg cttgtgatga ccaacgattc
    35221 tgacaatcct agctttaaga cggctagtgg caagcttatt aagtgggctg gtgattttat
    35281 cacagttgat actgctaagg ggcagaatgt cggtgttgtt ttaggtgctg gcataacgtc
    35341 cttgaaattt gaaacagttt gggggtgggc atagttgctc tttttacttg ataaagatgt
    35401 tagaacggtc aaatggaatg gcattccact tcacgaggcc agctctgcca ttgtcaaaga
    35461 agaaaccaac agtgatttct acctgactgt tcgctatcct atcacggatt caggtatcta
    35521 tcaacttatc aaagaggata tgctgattaa ggcgcctacg cctgtgctgg gtgctcagct
    35581 gtttagaatc aagaaaccta ttgagaatga tgatagcttg gatatcactg cttatcatgt
    35641 ttctgacgac atcatgaagc ggtctatcac tcctgtaagt gtagttggtc aaggttgtgc
    35701 tatggcactg tctcagatgg ttcaaaatgc taagactggt ctaggtgatt tttcgttcac
    35761 cagcgatatt atggacagca gaaccttcaa cacgacagag acgaaaacac tgtattcagt
    35821 ccttatggac ggtaaacaca gcattgttgg aacgtgggag ggtgagcttg tccgtgacaa
    35881 ttttgctctt tctatcaagc gtagccgtgg ggctgatcgt ggtgttgtca tcacaacaca
    35941 caagaacctc aaatcctatc aacgaaccaa gaactctcag ggtgttgtca caaggattca
    36001 tgcacggtca accttcaaac ctgatggagc tgaagatgaa gtgacgctca gagtgtctgt
    36061 tgacagtcca ctaataaact cttatcccta catcaatgag aaagagtatg agaacaacaa
    36121 cgctgaaacc gttgaagacc ttagaaagtg ggctgaggct aagtttacaa atgagggcat
    36181 tgataaggtt tctgatgcca ttgagattga agcctatgag cttgacggtc aagttgtaaa
    36241 tctgggcgac acggttaacc tcaagagtag aaagcacagt gctgacctct acaagaaggc
    36301 cattgcctac gagtttaacg ctttaacgga agagtatatc tccataactt ttgatgataa
    36361 gccaggagtt ggtggttctg gggtgtcaag tggcttgtct aatgttgctg atgctatact
    36421 tgtagcaagt gccacagctc aggacgttgc tgttcaaagg gctgtgaaaa atgctaacgc
    36481 tgcctttgat gcagagtttg gtaaaacaaa aacaaaaatt aatgatgata tcgaaatcgc
    36541 aaaagccaaa gtagaaagct ttaaatcgga actatctaat cgcatggata atcagcttct
    36601 tccccttgcg acagaagcta agaatttagc cagtcaggca caagcagacc tgaccagaaa
    36661 agaaatagag cttcgagctg aattaaatag acaagtcaca agcacagaaa ctgtaaaaat
    36721 ctctctaaca aatcttagtc acaatatgga tatcattaag caaaaagccc taaacgacct
    36781 tagagacgca gagacaaggc taaaggaagc tgatagcgtc caacaacttg cgacaaagcg
    36841 ggttgaggat aaactgacag gacttagcac gaaactggag tcattctctg ttggtggcta
    36901 taattatgtc attgacggcg gagaacctaa agaactaatg gctaacttct atggtaagac
    36961 ctatgatatt aatccccaac tactcgaacg aactagtcaa gcgaccttga gttttagcta
    37021 tgaagcagaa tcaactagtc gtttagaagt tagactttat aaaaagatgc acaccgggga
    37081 cactagtaag attacaatta ttgtcatgcc taactttgac ttatctccag ggaaaggatt
    37141 tatctctcag agctttgatt taggtggtgt tatgcctgac cctagaaacc aagcctggct
    37201 tgtgatgcgg gggacaaatg ctaacccctt gacgctctcg aaagttaagc tggaacgtgg
    37261 taccgttgct acagactgga ataatcgcga tgaaacctta aaagctagct tcgccgaata
    37321 caagcagacc gttgatgaaa acttagccaa ccttagaaca agtacggaaa ccttggcagg
    37381 tcaactgaca agtgctgagt ctagcattag gcagacgtcg gaatccttta gcaatcgttt
    37441 ggttagtctt gagacctata aagatagtga atcaaatcga gctagtcgtt actttgaagc
    37501 aagcaagtct gaaaccgcta agcagctatc agccttacga acagaagtta atggctctta
    37561 tgtggataag tcaacctatg aggaaaatgc aagaggtttg acaagacgct ttgaaagtct
    37621 gtcatcaggg acacagaact atgctttggg gactgaaaga gatgtctatc taaacgtttc
    37681 agggactacc gtctatgacc tttatagctt cggcactacc ttagcaataa aagggattga
    37741 aatgggagat actgtaacta ttgcatttaa ctattttgcg gatagtcgga cggcttttgg
    37801 ttcttatgaa ctggagctat acggacatgc aggcaaaatc gaacgggttg gtgaggtaca
    37861 gagaacgata tcaaatcgtg gtacctatac cgcatctttt gtggcaaaca atgccaattt
    37921 cagggcaaac tcccttaaaa ttagatttac ggatagccga cttaagttta gagtgacaac
    37981 acttcgtgtg actaagggga caattcctgc ggactggagt ccatctccag atgacctaaa
    38041 agcctactcg gatactaagc ttgaacagac agctaatgag attaaagcta gtgtaacaag
    38101 tcttgatcat aaaactctta agcaaactga tattactatg acttctgaag ggattgtcct
    38161 aagagcaggc aagacgagta atgatgtggc aagagctatt ggctcttact ttaaggtcac
    38221 tcctgatgct atagctctgt ttagctcact gataaaagtt agcgggaaca tgttggttga
    38281 tggttctgta actagtcgta atttggtaac aggcgctgtc gaaacgggac atgttaaggc
    38341 tggagcaatc acaggagtgc ttttagcagc tgaagctgta acggcagaaa aacttaaagt
    38401 cgaccaagct ttctttaaca agttgatggc caacgatgcc tacttgaagc aactatttgc
    38461 caagtcagcc ttcatcactc aggttcaatc cgtgacaata tctgccagtc agatttcggg
    38521 cggtgttatt aaagctttga ataatgccat ggaaatccag atgaacagtg gtcagatact
    38581 ttactacact gaccaagctg cactgaaacg ggttttgagt ggctatccta ctcagtttgt
    38641 taagtttgca actggtacag tatctggtaa aggaaatgct ggtgttacgg tcattggttc
    38701 taaccgctac ggtacagaat caaccaatga tggtggattt gttggagtcc gtgcatggaa
    38761 cggctcaaac attgactcac ttgacttagt tggtgataaa atccgattag caagctctgc
    38821 ctttgacaat tcagatggtt gggatgttcg gacacttgat tcaggcttga aaatcacacc
    38881 acacaatagg gctgctgaac gcaatagccg aattgaggtt ggtgatgtct ggattttaaa
    38941 aggaaatgga tcctattctt ctctcagaga tattttgaac tcattcaatg agaacttctc
    39001 aaaaggccca aatgctgact cttacactta ctaccattcg gggttctaac tagaaaggaa
    39061 caatcatgaa ccaagaacaa caactcaatc aagcgctacg cttgaccgtc aatgaattga
    39121 cagcccagtt ggccaatgag tcaaccacta agaacttgct ggctatccag ctgactgaag
    39181 ttgatcagga gaaacagcaa ctgactcagc aaaatgctga acttcaagca agagtttccg
    39241 aactagaagg cttgcttgat gaacaaactc aaccagaaat cattgaagga gaataaccat
    39301 gactacaaca gaaaataatt tacttgacct tgaaacaatc acagaaccct ttgacttggc
    39361 gacagcactc aagtacatga aggaaaatgg tgagttaatc cgttgcaaga atgctgtcaa
    39421 tgacttctac atgtaccgtg acatgcagaa acgtcctgtc atcgtcaatg gtcgccgtca
    39481 attcaaggac gtggaaactg tctgggcatt taaccaatgg ggcgggacaa cacccacaat
    39541 caacatggct gatctcttta atcttgaata ctacatcatg acctttgatg agaacggaaa
    39601 tcctgactgg actgagcctc acttggagga taagtaagct catctaaaat aaacaggata
    39661 ggagatagtg aatgccacca tggttaatag atagcactgt ggttgtggcg atggtgacgg
    39721 tgctgggcgg tctgttcagc actattatca ccacttcagc caatcggaaa gaccagctga
    39781 taaaacatca gtatgaggat atcaaggaag atttgtctgg cttgattgac aaggttaaga
    39841 cgattgatca tacaaccacg gaaacaaaga aaatttcaga aatcacaaaa gacggtacgc
    39901 ttaaaatcca gcgttaccgt ctttttcacg atttgacaaa ggaaatttct caaggctaca
    39961 caactattga acatttcagg gagttgagca ttctctttga gagttatcaa ctgcttggcg
    40021 gaaatgggga aattgaagcc ttgtttgaga aattcaaaca gttaccaatt gaggaggact
    40081 aaacatgaca caaattacag atattatcat cagctcagct atgggaattt tgactatttt
    40141 ggcaggaatt gcagtgcaag ctgtcaaggt ttatttgatt aaaaaaggcg gtgaaaaggc
    40201 cgtcttgatt acagaaatct tggccaagaa cgctgttaat gctgtggagc aagtggccac
    40261 tgaaactggc ttcaaaggag ctgacaagct aacttctgct aaggctcaga ttttagctga
    40321 gttgcaaaaa tacaatatcc acatgtcaga cgatgatctc actttgtttg ttgaatcatc
    40381 tgtcaaacaa atgcacgatg cctggaagga gtaacctatg gaaatcaaca cagaaacagc
    40441 cattgcctgg atgtctgcac gtcaaggcaa ggtcagctat tccatggact accgtgacgg
    40501 acctaacagc tatgactgtt ccagctctgt ctattatgct ctcaggtcag ctggtgcaag
    40561 ctcagcaggt tgggcggtca acactgagta catgcacgat tggctgatta aaaacggtta
    40621 tgagcttatt gctgaaaacg tggattggaa tgctgtccgt ggtgatatag caatttgggg
    40681 catgcgaggg cactcaagcg gagctggtgg tcatgtagtc atgtttattg accctgaaaa
    40741 tatcattcac tgtaactggg caaataatgg catcacagtg aacaactaca atcagacagc
    40801 ggctgctagt ggctggatgt attgctacgt ttaccgtttg aaaagcggag cttctaccca
    40861 aggaaaaagc cttgatacct tggtcaagga aacccttgct ggtaactacg gtaatggcga
    40921 agcacgcaag gcagtgcttg gcaatcaata tgaggctgtt atggcagtca tcaatggcaa
    40981 aactacgact aatcaaaaga ctgttgacca acttgctcaa gaggtaatcg ctggcaaaca
    41041 tggcaacggt gaagctcgta aaaagtcgct tggtagtcaa tatgatgcag ttcagaaacg
    41101 agtgacggaa ttgctcaaaa aacagccctc tgagccgtct aaggctcaag aggtaaacaa
    41161 gcccacggaa accaaaacaa gccaaacaga gctaactgga caagccacag ccaccaaaga
    41221 agagggcgac ctctctttca atgggactat cttgaaaaaa gcggtgctgg acaagattct
    41281 gggcaactgt aaaaagcatg atattcttcc aagttatgca ctgactatcc tacactatga
    41341 aggtctttgg ggaacttcag cagttgggaa ggcagataat aactggggcg gtatgacctg
    41401 gactggtcaa ggcaaccgtc caagcggtgt cactgtcaca caaggttccg cacgtccttc
    41461 aaatgagggc ggtcactata tgcactatgc aagcgtggat gactttctga cagactggtt
    41521 ttatcttcta agggctggtg gctcttacaa ggtgtctggt gctaaaacct tcagtgaggc
    41581 tatcaaggga atgtttaaag ttggcggtgc tgtctatgat tatgctgcaa gcggctttga
    41641 tagctatatt gtcggagctt caagccgcct caaggctatt gaggcagaaa atggttcact
    41701 ggacaagttt gataaaacta ccgacattgg tgacggtagc aaagacaaga ttgacattac
    41761 tattgaaggt attgaagtta ccatcaacgg tatcacttat gaactgacta aaaagccagt
    41821 ttagaattag atacgataag acaaaataag ccctcagcaa ttgccggggg ctgtttttcc
    41881 gttataacgg actttcctca aaatatcggt tataatggac tttttttgat aaaaatttta
    41941 aaaatatttg ttaaaaacaa gtgttttcta ttgacaagtg tttaaaacaa gtgtataata
    42001 caattaaaga taaggaaagg agataagcca atgacagagc gagagcttaa gaaaattgct
    42061 aagaagcaag gttttagtaa aactaacttt ggcaaagggt cacacgaagt ttggaaacat
    42121 ccagacggac ggacagtgac gatacctaag ccaaaggaaa aagattacag accaggcaca
    42181 ctgaacaaca ttctcaaagt cttgtatggg gagtgaggac gctcctcccc gtacccctaa
    42241 aaggggatac tctgatcatt ggcttaatct aatattatga aatacactta tttagcattg
    42301 tttgaagtgg ataaagaaaa cggtggctat aacatttctt tccctgattt tcctggagca
    42361 tttagtgaag ctgatagctt gaacgaggcc atttttaacg ctcgtgaagt ccttgaaatc
    42421 tatacaatta tgtttgaaga cgagggcaaa gagtttccta aagcatcatc attcaaggct
    42481 cttgcaagca atttagcaag tgacgaagat gtgattcagg ctatttcagt tgatactgag
    42541 cttgtccgtg agcgtgaacg ctctaagatt gtcaacaaga ctgtcacact gccaagctgg
    42601 cttgtggaag ttggaaaaga aaacaaggtc aactttagcc agctgttgca aaaagcaatc
    42661 cgtgaggaat tgcaagtata agaaagagcg gtcaagatgg ccgctttttt ctgtataaaa
    42721 aattatgaaa atagatagta atacttttca aaaattgacc tatgaaaaac cgtccgcccc
    42781 aaattcgccc caaattattt ttaaagttag cctgatttaa cctgatggaa aatcaaaaaa
    42841 gcctataaaa tcaggctttt cattcggtta attcctgtta attcaggtac tgaaaggcgg
    42901 tagacggatt tgaaccgacg atcaagcttt tgcagagccg tgccttacca cttggctata
    42961 ccgccataac agataatatt ctatcgaaaa aaaaaagatt cgtcaagtct ctttcgtaaa
    43021 atagccaatt ttagccctta aacttcctca tattaccaaa aaaagagatt gatacaatgg
    43081 gtatcaatct cttttagatt attgtctgat taaataatca aatgcgccaa tggcagctgt
    43141 ggcaccagac cccatggaaa taatgatttg cttgtaggca gcatctgtac agtcacctgc
    43201 agcaaaaata ccagggatat ttgttgagcc aaatttatca acgacaattt cttgacgttc
    43261 attgagttca atgccgctat ccttgagcca agaagtactt ggtacaagac caatttgtac
    43321 aaaaacacct tctaaatcaa tgtgtttttc ttcgtttgta tcacgatcgg tatagtttaa
    43381 accagtgaca tgatcttcac caacgatatc tttagtagca acattcttaa gaatagttaa
    43441 attgtcagtt ttagctgctc gttcttgcag tacttggtct gctttgagct caggtaagaa
    43501 ttctaggact gtaacatgtt tagtgacacc ggctagatct aaagcagctt ccatacctga
    43561 attaccacca cctataacag caacattttt tccttcgaaa agagggccat cacaatgtgg
    43621 acagtaagta acacctttat tgcggaattc ttcctcgcca ggaacgttga tatttcgcca
    43681 cttagcaccc agagctagga tagcagtttt agcttgtaaa atagctccgt tagccagtgt
    43741 tacctcaact aattcttttt tctcaatgcc agttgctaat tgactcttga taatatcaat
    43801 atcgtaagat ttggtatgct cttcaatttg agccattaat ttagggcctt cggtgtacag
    43861 tgtaccaatc atgttctcaa taccaactgt ttcgataact tgtccaccaa aagtttctgc
    43921 taaaatacct gtttttaacc ctttacgagc ggcataaatt gcagcactgt tacctgcagg
    43981 accaccacca ataactagaa catcatagac acctttgtca gcaaatgctt cagcatcaag
    44041 aggtccatct agttgttcta aaagctgttc aattgtagca cggccagaag taaattcttc
    44101 ttggtcctta taaacagtag gaacagacat gatacctttt gatttgactt cgtcttgata
    44161 catgcctcct tcaatcatag tatgagtgat atttggattc aaaacagcca ttatattaaa
    44221 ggcttgaacg acatctggac aattatggca agttaatgaa acataagttt ctagattgat
    44281 agttttctca atccccttaa ttcgtttgat aatatcttca tcaacttttg gtgcgcgacc
    44341 agagacttgc aacaaagcta aaatgaaaga tgtgaattca tgccccattg gcaagcctga
    44401 aaaaataacg cgactttctt gccctttttt tgcaatccca aaactaggtt gacgttttaa
    44461 atgtgttgat tcaaggctga tacgatcaga catagcgaca atttcatcta gaaaatcctt
    44521 tactttttga gaattatcgt tatctcctaa atcagtttgt aatacaatat cagattctag
    44581 caaatctaaa tattgcgcta attgagcctt gatttcttta tctaagacca tggattacct
    44641 cctggttatt aaattttacc tacaaggtct aaactaggag ttaaagtttc agctccttct
    44701 ttccatttag ctggacaaac ttcacctgga tgttggcgga tgtattgagc agcacggact
    44761 ttatcaatga gagtacttgc atcacgtccg atgccatcag cattaatttc catcatttgg
    44821 atgacaccgt ctggatcaat gataaatgta ccacgttgtg ctagaccatc ttgtccaagg
    44881 acatcaaaac cttgtgaaat aaggtgtgaa gggtcaccaa tcataggata tgtgatagta
    44941 ccaacgacat ctgagtcatc atgccatgct ttatgaacaa aatgagtatc agtagagaca
    45001 gaataaactt ctacatcaag agatttaagt gtttcgtatt gctcttgaag gtcaccaagt
    45061 tcagttgggc aaacaaatga aaagtctgct gggtagaaac aaaaaactgc ccattttcct
    45121 ttaacgtctt catttgtaac agtgatgaat tttccatcgt gataagcttg agctgaaaat
    45181 tcaataattt cttttccgac taatgacata ataatgtcct cctttatttt ttaatagcct
    45241 aattataaac gaaatacttt taaatctcca ataaaaacgt ttacattttt ctaagaaaag
    45301 tatgattttt ctaatagagt ggaagatttt atagcttgtc aaatctaact ttttttgata
    45361 aactactata gttgcttaaa gtgacaaata ctcatcttag atcaattgtg gtcttgttgc
    45421 cgaaaggtta ggctgcaagt cgaagtctga gagaggaaaa aacattttta aaggagaact
    45481 tactcatggc agtaatttca atgaaacaac ttcttgaggc tggtgttcac tttggtcacc
    45541 aaactcgtcg ctggaaccct aagatggcta agtacatctt cacagaacgt aatggtatcc
    45601 acgttatcga ccttcaacaa actgtaaaat tagctgacca agcttacgaa tttgttcgtg
    45661 atgctgcagc aaacgatgca gttatcttgt ttgttggtac taagaaacaa gcagctgagg
    45721 ctgttgctga agaagctaaa cgtgctggtc aatacttcat taaccaccgt tggttaggtg
    45781 gaactcttac taactgggga actatccaaa aacgtattgc tcgtttgaaa gaaatcaaac
    45841 gtatggaaga agaaggaact tttgaacttc ttcctaagaa agaagttgca ttgttaaaca
    45901 aacaacgtgc acgtcttgaa aaattcttgg gtggtatcga agatatgcca cgcatcccag
    45961 atgtaatgta tgttgttgac ccacataaag agcaaatcgc tgttaaagaa gctaaaaaac
    46021 ttggtattcc agttgtagct atggttgaca ctaacgctga tccagatgat atcgatgtta
    46081 tcatcccagc aaacgatgac gctattcgcg ccgttaaatt aattacttct aaattagctg
    46141 atgcagttat tgaaggtcgt caaggtgaag atgctgacgt tgacttcgct caagaagcac
    46201 aagcagattc aattgaagaa atcgttgaag tagttgaagg atcaaacaac gactaatatt
    46261 taatttattg taaatcctaa aacggggcag atgcaaaccg ctctgtctcg tttttttaat
    46321 cattatttga aattaagagg agaacgaaaa tggcagaaat cacagctaaa cttgtaaaag
    46381 aattgcgtga aaaatcaggt gcaggcgtta tggacgctaa aaaagcatta gtagaaactg
    46441 atggtgacct tgataaagcg attgaattac ttcgcgaaaa aggtatggct aaagcagcta
    46501 aaaaagcaga ccgtgttgct gctgaaggtt taacaggtgt ttatgttgat ggtaacgttg
    46561 cagcagttat tgaagttaac gctgagactg actttgttgc taaaaacgac caatttgtaa
    46621 cattagttaa tgaaactgct aaagttattg cagaaggacg tccttcaaac aatgaagaag
    46681 cacttgcttt aacaatgcct tcaggtgaaa ctttagaaca agcatttgta actgcaactg
    46741 ctacaattgg tgaaaaaatt tcattccgtc gcttcgcttt agttgaaaaa actgatgagc
    46801 aacattttgg tgcataccaa cataatggtg gacgtatcgg agtaattact gttgttgaag
    46861 gtggcgacga tgcacttgct aaacaagtat caatgcacgt tgcagctatg aagccaactg
    46921 ttctttcata tactgaactt gatgcacaat ttgttcatga tgaattagca cagttgaacc
    46981 acaaaattga gcaagacaac gagagtcgtg ctatggttaa caaaccagct cttccattcc
    47041 ttaaatatgg atcaaaagct caattgactg atgaagttat tgcccaagca gaagaagaca
    47101 tcaaagcaga attagctgca gaaggtaaac cagaaaaaat ctgggataaa atcgttccag
    47161 gtaaaatgga tcgtttcatg cttgacaaca ctaaagttga ccaagagtac acactccttg
    47221 ctcaagttta catcatggat gatagcaaaa cagttgaagc ttatcttgag tcagtaaatg
    47281 ctaaagcagt tgctttcgta cgttttgaag ttggagaagg tattgagaaa gcttctaacg
    47341 actttgaagc agaagttgca gctacaatgg cagcagcact tgaaaaataa ttaacactcg
    47401 ctttatatta taatttggca gcaaaatgtt gtcagtaaat atattttaaa ataagacact
    47461 tggcttaagt caagtgtctt attttgctat cgaattggtg gattggcaaa tattaaaaag
    47521 gagagacagt catctcacct taacttttct agaataggtt gaagccgata atggataatg
    47581 ttcccatgat gcatccagct agtataacac ctagcataac agcaataaca ctatgtttaa
    47641 attcccaatc taggaggcta gctgcaagtg tgccagtcca tgctcctgtt ccgggtaagg
    47701 gaatgcctac aaacaatagc aaggcaataa acaatccttt ttctccagct actttttcaa
    47761 gtttttgacc accgctatgt ccttttttaa ggcaccatgt gaaaaactta ccagtataag
    47821 gtttatctgc tccccattct agaactttgc gagcaaagaa gaagatgata ggtacaggta
    47881 gcatgtttcc aacaacaccg atagctagcg cttcccacaa aggtattcca ttagcgattg
    47941 caaatggcac agctcctcgt agttccacta agggaatcat tgaaatcaaa aatgtaataa
    48001 tgtaattcat taaataaaac ctttctatgg tattattcca ttttatctta tcttgctagt
    48061 aatgacaact aaaatccgcg gtcattctag ttttactatc cttagataga tttcatttcc
    48121 gtatctttta aaaatttgat ataataatag tcagttatag tcaaagagga aggagtgaat
    48181 ttgtggccat aaaaaatact tccgataata ttgaagaata tataaaatca ttattagaac
    48241 aatcaggaat tgctgaaata aagcgttcca atttagctga tacttttcaa gttgttccta
    48301 gtcagattaa ttatgtcatt aagacacggt ttactgaaag ccgtggttat gtggttgaga
    48361 gtaaacgagg tggtggaggt tacatccgca ttgctaaggt tcatttttct gaccaacacc
    48421 aattatttgg aaatatgtta tcaacgattg gcgagcgtat cagtgaacaa gtctttgatg
    48481 atttgattca attattattc gatgaagaga taattacaga acgtgaagga aatttgattc
    48541 tggcgacatc aggtgatgac gtcttaggag agcaagcctc tgttattaga gctcgtatgc
    48601 tccgtaaatt attacaacga ttagatagaa aaggattcca tttatgagtc attattccat
    48661 aaaattacaa gaagtattca gattggcgca atttcaagca gcacgctatg aaagtcacta
    48721 tttagaaagc tggcatcttc ttttagcaat ggttttagtt catgattcag tagctggttt
    48781 gacatttgct gaatatgagt ctgaagttgc aattgaagag tacgaagcag caacaatttt
    48841 agctttaggt agagcaccga aagaagagat taccaattat caatttttag agcaatcacc
    48901 agctttgaaa aagatcttaa agttagcaga aaatattagt atcgtagtag gagctgaaga
    48961 tgtgggtact gaacatgtct tgcttgctat gttggttaat aaagatttat tagccacacg
    49021 tatcttagaa ttagttggct ttagaggtca agacgatggt gaatcagttc gtatggttga
    49081 tttacgtaag gctctagaac gtcatgccgg ttttacaaaa gatgatatca aagcaattta
    49141 tgaattacgt aatcctaaaa aagcaaagtc gggtgcttct ttttctgata tgatgaaacc
    49201 accaagtact gctggtgatt tagctgattt cacacgtgat ttatcacaga tggcggttga
    49261 tggggaaatt gaacctgtta ttgggcgtga taaagaaatt tcacgtatgg tccaagtctt
    49321 aagtcgtaaa acaaagaata atcctgtctt agttggagat gcgggtgtcg gtaaaacagc
    49381 tttggcgtac ggtctagcac aatgtattgc taatggaaat attccttatg agcttagaga
    49441 tatgcgtgtt ctagagttag atatgatgag cgttgttgct ggaactcgtt ttcgtggtga
    49501 ttttgaggaa cgtatgaatc aaatcattgc agatattgaa gaagacggtc atattattct
    49561 tttcattgat gaactgcata ctattatggg ttctggctct ggtattgata gcacacttga
    49621 tgctgctaat attttaaaac cagctttggc tcgaggcact ttacgcacta ttggagcaac
    49681 aactcaagag gaatatcaaa agcatattga aaaagatgct gctttatctc gtcgctttgc
    49741 aaaagtttta gtagaagaac caaatcttga agacgcatat gaaattctac ttggattgaa
    49801 gccagcgtat gaagcctttc ataatgttac catttcagat gaagcagtta tgactgctgt
    49861 aaaagtggct catcgttatt tgacaagtaa gaatttacca gactcagcta tagacttact
    49921 tgatgaagct tctgccactg tgcaaatgat gattaagaaa aatgctccat cgcttttaac
    49981 agaagttgac caagctattt tagatgatga tatgaaatct gcttctaaag ctctaaaagc
    50041 aagtcataag gataaaaagc ataagcctat tgccgtaacc gaagatcata ttatggcaac
    50101 tctcagccgt ctatctggta ttcctgttga aaaattgaca caagctgata gtaaaaaata
    50161 cctcaattta gaaaaagaat tgcataaacg tgttatcggt caagatgatg cagttactgc
    50221 cattagtcgt gctatccgtc gcaatcaatc tggcattcgt actggtaagc gtcctattgg
    50281 ttcctttatg ttcttgggac caactggtgt tggtaagaca gagttagcaa aagccttggc
    50341 agaagtgttg tttgatgatg aatcagccct aattcgtttt gatatgtcgg aatatatgga
    50401 aaaattcgca gcttctcgcc tgaatggggc acctccaggt tatgttggtt atgatgaagg
    50461 aggagagctg actgaaaaag tacgcaataa accatattca gttttacttt ttgatgaagt
    50521 tgaaaaagct catccagata tttttaatgt attattacaa gtgcttgatg atggcgtttt
    50581 aacagatagt cgaggacgaa aagttgactt ttcaaatact ataatcatta tgacttctaa
    50641 tttaggagca actgcattac gtgacgataa aacagttggt tttggagcta aggatatcag
    50701 ccatgattat actgctatgc aaaaacgtat tatggaggag cttaaaaaag cttatcgacc
    50761 agagtttatc aatcgtattg atgagaaagt tgtattccat agtctcagtc aagataatat
    50821 gcgtgaagtt gtgaaaatta tggttaaacc tttaatttta gcgcttaaag ataaaggtat
    50881 ggatctgaaa ttccagcctt cagccttgaa acacttggca gaggatggct atgatataga
    50941 aatgggtgct cgtccactgc gtagaactat tcaaacgcaa gtagaggatc atttatcaga
    51001 actactattg gcaaatcaag tgaaagaggg gcaagtaatt aagattggcg tatccaaagg
    51061 caaattgaaa tttgatattg caaaatcata aaacattcca gtaccgatgg g
//
LOCUS       DATRME010000007        46451 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRME010000007 DATRME010000000
VERSION     DATRME010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46451
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..442)
                     /locus_tag="VBC23_000415"
     CDS             complement(<1..442)
                     /locus_tag="VBC23_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6842388.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDE"
     gene            complement(439..960)
                     /locus_tag="VBC23_000416"
     CDS             complement(439..960)
                     /locus_tag="VBC23_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6842389.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(942..1916)
                     /locus_tag="VBC23_000417"
     CDS             complement(942..1916)
                     /locus_tag="VBC23_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6842390.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(1913..2707)
                     /gene="racE"
                     /locus_tag="VBC23_000418"
     CDS             complement(1913..2707)
                     /gene="racE"
                     /locus_tag="VBC23_000418"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6842391.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(2882..3121)
                     /locus_tag="VBC23_000419"
     CDS             complement(2882..3121)
                     /locus_tag="VBC23_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6842392.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(3279..3821)
                     /locus_tag="VBC23_000420"
     CDS             complement(3279..3821)
                     /locus_tag="VBC23_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842393.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFMSVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(3883..4404)
                     /locus_tag="VBC23_000421"
     CDS             complement(3883..4404)
                     /locus_tag="VBC23_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6842394.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(4472..5161)
                     /locus_tag="VBC23_000422"
     CDS             complement(4472..5161)
                     /locus_tag="VBC23_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6842395.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIMSIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(5184..5687)
                     /locus_tag="VBC23_000423"
     CDS             complement(5184..5687)
                     /locus_tag="VBC23_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6842396.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(5725..6468)
                     /locus_tag="VBC23_000424"
     CDS             complement(5725..6468)
                     /locus_tag="VBC23_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6842397.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            6500..6778
                     /locus_tag="VBC23_000425"
     CDS             6500..6778
                     /locus_tag="VBC23_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6842398.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            6863..7795
                     /gene="yidC"
                     /locus_tag="VBC23_000426"
     CDS             6863..7795
                     /gene="yidC"
                     /locus_tag="VBC23_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6842399.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(7995..8660)
                     /locus_tag="VBC23_000427"
     CDS             complement(7995..8660)
                     /locus_tag="VBC23_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6842400.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(8684..9541)
                     /locus_tag="VBC23_000428"
     CDS             complement(8684..9541)
                     /locus_tag="VBC23_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6842401.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(9702..11162)
                     /locus_tag="VBC23_000429"
     CDS             complement(9702..11162)
                     /locus_tag="VBC23_000429"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6842402.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(11266..11748)
                     /gene="greA"
                     /locus_tag="VBC23_000430"
     CDS             complement(11266..11748)
                     /gene="greA"
                     /locus_tag="VBC23_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6842403.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(11821..13623)
                     /gene="mltG"
                     /locus_tag="VBC23_000431"
     CDS             complement(11821..13623)
                     /gene="mltG"
                     /locus_tag="VBC23_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6842404.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKI
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(13710..14213)
                     /locus_tag="VBC23_000432"
     CDS             complement(13710..14213)
                     /locus_tag="VBC23_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6842405.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(14299..15630)
                     /locus_tag="VBC23_000433"
     CDS             complement(14299..15630)
                     /locus_tag="VBC23_000433"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6842406.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(15640..16257)
                     /locus_tag="VBC23_000434"
     CDS             complement(15640..16257)
                     /locus_tag="VBC23_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842407.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(16422..19520)
                     /locus_tag="VBC23_000435"
     CDS             complement(16422..19520)
                     /locus_tag="VBC23_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6842408.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGNLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(19675..20985)
                     /gene="der"
                     /locus_tag="VBC23_000436"
     CDS             complement(19675..20985)
                     /gene="der"
                     /locus_tag="VBC23_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6842409.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(21033..21935)
                     /gene="dnaI"
                     /locus_tag="VBC23_000437"
     CDS             complement(21033..21935)
                     /gene="dnaI"
                     /locus_tag="VBC23_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6842410.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(21932..23107)
                     /locus_tag="VBC23_000438"
     CDS             complement(21932..23107)
                     /locus_tag="VBC23_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6842411.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVITAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(23107..23586)
                     /gene="nrdR"
                     /locus_tag="VBC23_000439"
     CDS             complement(23107..23586)
                     /gene="nrdR"
                     /locus_tag="VBC23_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6842412.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(23735..25240)
                     /locus_tag="VBC23_000440"
     CDS             complement(23735..25240)
                     /locus_tag="VBC23_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6842413.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAVAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(25230..25919)
                     /locus_tag="VBC23_000441"
     CDS             complement(25230..25919)
                     /locus_tag="VBC23_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842414.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(26154..26687)
                     /locus_tag="VBC23_000442"
     CDS             complement(26154..26687)
                     /locus_tag="VBC23_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6842415.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(26792..27682)
                     /gene="htpX"
                     /locus_tag="VBC23_000443"
     CDS             complement(26792..27682)
                     /gene="htpX"
                     /locus_tag="VBC23_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6842416.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAVIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(27703..28257)
                     /locus_tag="VBC23_000444"
     CDS             complement(27703..28257)
                     /locus_tag="VBC23_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6842417.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            28385..29098
                     /gene="rsmG"
                     /locus_tag="VBC23_000445"
     CDS             28385..29098
                     /gene="rsmG"
                     /locus_tag="VBC23_000445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO6842418.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            29113..30492
                     /locus_tag="VBC23_000446"
     CDS             29113..30492
                     /locus_tag="VBC23_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6842419.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKEPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            30505..31176
                     /locus_tag="VBC23_000447"
     CDS             30505..31176
                     /locus_tag="VBC23_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6842420.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(31211..32041)
                     /locus_tag="VBC23_000448"
     CDS             complement(31211..32041)
                     /locus_tag="VBC23_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6842421.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(32034..33710)
                     /locus_tag="VBC23_000449"
     CDS             complement(32034..33710)
                     /locus_tag="VBC23_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842422.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(33727..34275)
                     /locus_tag="VBC23_000450"
     CDS             complement(33727..34275)
                     /locus_tag="VBC23_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6842423.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(34444..35652)
                     /gene="sstT"
                     /locus_tag="VBC23_000451"
     CDS             complement(34444..35652)
                     /gene="sstT"
                     /locus_tag="VBC23_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6842424.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            35828..37195
                     /gene="brnQ"
                     /locus_tag="VBC23_000452"
     CDS             35828..37195
                     /gene="brnQ"
                     /locus_tag="VBC23_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6842425.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            37309..38364
                     /locus_tag="VBC23_000453"
     CDS             37309..38364
                     /locus_tag="VBC23_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6842426.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(38413..39105)
                     /locus_tag="VBC23_000454"
     CDS             complement(38413..39105)
                     /locus_tag="VBC23_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842427.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(39102..40172)
                     /locus_tag="VBC23_000455"
     CDS             complement(39102..40172)
                     /locus_tag="VBC23_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6842428.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(40165..41541)
                     /locus_tag="VBC23_000456"
     CDS             complement(40165..41541)
                     /locus_tag="VBC23_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6842429.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(41675..42499)
                     /locus_tag="VBC23_000457"
     CDS             complement(41675..42499)
                     /locus_tag="VBC23_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6842430.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(42640..43473)
                     /locus_tag="VBC23_000458"
     CDS             complement(42640..43473)
                     /locus_tag="VBC23_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6842431.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(43575..44264)
                     /locus_tag="VBC23_000459"
     CDS             complement(43575..44264)
                     /locus_tag="VBC23_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6842432.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(44419..45135)
                     /locus_tag="VBC23_000460"
     CDS             complement(44419..45135)
                     /locus_tag="VBC23_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6842433.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(45561..46265)
                     /locus_tag="VBC23_000461"
     CDS             complement(45561..46265)
                     /locus_tag="VBC23_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842434.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
BASE COUNT        14676 a         9203 c         7136 g        15436 t
ORIGIN      
        1 attcgtctgt aaaatcatgt aggaggctat taatagcttt taaaatcgat ttacgggtta
       61 taatgcctct aaaatcattt tgatcactga taacaggtaa aaatggataa tctacaatct
      121 tatgcataat tgctgtcaga gtagctgcct cattaatcgg ctcgattttt gtattaacca
      181 tctcaacaat atcagtctgt gccatctccc aatctgttaa ttgaccttta gattggtagc
      241 ccataatgtc tgagatacta atagtaccta catatttttt ctctttagtg ataactggta
      301 cacgtgaaaa accattactt gctaatagta acataacgtg gtctgcattg tgtgtatcca
      361 caaaaatagc aacgtcttca gctggaatta gatagtggtc caaatgattt agcaaaaacg
      421 actcaaattc tttagcaatc atcttgaaaa ttcctttgtc aagttaggat agggttggtg
      481 ttggcgagta taatactcta ccaaaacctt actatctgtt atagttacaa cagcgtaaag
      541 cttctcattt attggtcctc ttggctgcaa aacactacct ggattgataa aaatagtttt
      601 accatttttc caagcatctg cacgatgaag atgaccatat aaacaaatat ccgcgtcttc
      661 ttgctgagct aataaatcca atttatccca ggtaaaatta atctgatgga gatgaccatg
      721 ggtttgaaca ataacagcat tatctatttt tgtaaccaaa acttccggat aacctgaatc
      781 ataatcacaa tttcctgtga ccacatgaat gccttcccat ataggatctg agctaggaag
      841 ttcggaatcc ccattgtgaa aaatagcatc cacttttccc aaataatgat tctttatatc
      901 ttttacaatg tcacgatctc cgtgagaatc actcattaca actaattttc taattgccat
      961 tttggaaata cctccatcaa tttacggaca gcttgcccac gatgtgatag attatttttt
     1021 tcttgtgcgc tcaattcagc tgctgtccta ctaccttctc cgacaataaa gagcggatca
     1081 taaccaaagc cattttcccc cttaggttcc gtaccaatat aacctggcca ctctgcttca
     1141 actaccaaac tttctttatt tggagctgac acgactaagg ttgtatgaaa ctgtgctgaa
     1201 cgtctttctt tgtcaaatac catggctaat tcatgcaata atttggcatt attacgagca
     1261 tctgtcgcgt caggacctga aaaacgagca gaccataccc ctggcaaacc acctaaagca
     1321 tctactttta atccggaatc atctgaaatt accatttttc cagttaattt tgaaatagtc
     1381 tctgctttaa gtcgagcatt ttcttcaaag gtcataccag tctcttcaac ttctggtaaa
     1441 tctgggtaat catttaaatt ttcaactttg agtcctaatt tcccaaataa ttctctaaat
     1501 tccttcgttt ttccttcatt gtgagtcgca attaatatgg tatccccaaa attattatca
     1561 ttctttaaat caaagaattt ttctaaagaa gtgccctctt taggtaagtg aactaagaac
     1621 aactgatcat cttcactaac taagatggcc cctttatgtt gagtcacttt gatatgcctt
     1681 gaatactctt gattaatcat atcaacgagg aatgctaata attgaaaatc cgaagtcatc
     1741 tgcacaacgt gaagctgtag accttcaact ttcaaagttg ataatagacg atgaaaaaca
     1801 tcttggatag cttcgtaaga ttcatcatta ccaaaacaag taaaataatt aaaagcttgg
     1861 aaactaccta aaaaccaatt gccttcagtt ttactttcaa aaattgtttt tgtcataata
     1921 cgatacgctc cacatttatt tcttgactaa gccattgttc tgcaatttct ttaaaacctt
     1981 ttgggctggc ggttgtgtaa aagtgatgac caccgtgttt attttgccaa ttatggttta
     2041 tctcaaaata gttcaataaa acagaaatat cacgaacggt ttctgcgcca ctatcaatta
     2101 atttaacctc agcccccata acattttgaa tgatgggacg taatagggga taatgcgtgc
     2161 aacctaaaat taaagtatct aatttaccaa ctaatgggga caacgtttca taaaccacct
     2221 ttttggctaa actagaagac atctgatttg attccacaat tggaacaaat ttcggacaag
     2281 caagggatac cacagcagta tttggagaca aagcttgaat tttttgacga taagcatctg
     2341 atttaacagt catgggagta cctataatac caactttccc taaattagtt gatttgatag
     2401 ctgcgctagc tcctggtaaa ataacgccta aaacagggat gtctagtttt tctttaattt
     2461 cttgccaggc aactgcagtt gctgtattac aagctataac aatcatctta acatttttag
     2521 tcaataagaa gttaaccatc tgccaggtaa actctctaat ctgttgagca ggtctaggac
     2581 catacggagc tctagcctga tctccaatga agattacttc ctcttctgga agttgacgga
     2641 acatttcctt aacaaccgtt aaaccaccta caccagaatc tagaaagcca ataggcctac
     2701 tatccatttt tattcctcca atattagtga ctaaaagagg ctggacaaca gtcctagcct
     2761 ctctatacaa taaccaatca gtcaaatcta cacttgacca agtttgccca aatcttgtct
     2821 agaaatctga tggactatca ctcactattt catcactaaa cgcttcaatg ctaaaacact
     2881 cttatttttt agttttgata gctgtttttg cgtgttttac aatattacgg tatgtttgtt
     2941 gaactttagc ttcactaggt ttttgtccca tttgactcat catttcacga atagcatccg
     3001 gtgttaaacg agggtgctct ccaatctctt tttcaatttg tttacgagca ataaaaatgc
     3061 ctcccacgag accaccaaac aaagcgacga taattaataa aatccaaata gttgttgaca
     3121 tctattcatt ctcctcattt atttacatga ctcattatag caaaaaaaga aagagtttac
     3181 aaatatatgt aaacaaaatc aaagcctcta tcgtccttgt cacgtcaata cattattaaa
     3241 ttttcactag agcatggtta agcacatagt cgagtccttt atcttatcct aagtgttttc
     3301 cccaactttt gtgtcatcca tttccaaatg aagagagcta tgtaaaaatc taccaatcca
     3361 tgtagcaaag agcctagtcc tatcaaagaa aatagtgacc ataaataagt tgaactactg
     3421 ttagaagtca ttgtgattat ataaacagta ataaattcga gcagaccatg aattatgtta
     3481 atagctacag caaataacaa tgtttgccat ttactcatca ataaagaggg ctttgactta
     3541 atcaagaaag ccgcaattaa tgcaaatccg atatgtgata aagctctaag aacgattaca
     3601 atcggaaaac cagctagtag aaatccaagt cctgtcccta aagctaccaa tattgcaact
     3661 ggaacagaca taaaaatact taaaaataaa ggtacatggc ttgctaaggt gaaagaagca
     3721 ggcccaataa ttattttagc aggcatgatt ataggaatca tgattgcaaa agctactaaa
     3781 atagaagtat aagaaaccat ttgtgttgtt ttttttgaca tttttatctc ctttgaagct
     3841 attatacaaa ataaccttac aggtgtaaag acacctgtaa ggttatttgt ctatgtataa
     3901 aattcctgct ttttttagag cctcacatat ttggtcaaaa actgactgat cttgacaaga
     3961 aatcatatgt gtatgtagac catctgtcaa agaggatagc aattctgcat tggaaacttt
     4021 taatttagca gtaaaattat caatgtcttt ttggctttga atgtttaagg gagcagtcag
     4081 catcccataa atagggtgct ctacctccac attcataatg attccttgat accgcaaaat
     4141 aatttccagt tcttcttctg tctgttctat gccatgctga caaaccaatc tagctgtgaa
     4201 ttgatgagta cttagagcac tggacataag gtatcctttt ggggtagaaa taatatcaca
     4261 ttgttgtgca cgcaataagg caatatctcc aacaatcacc tgcctactaa cactaaaaat
     4321 ctttgctaat gtgcttgccg atatagcttc ttttgttcct tttaaagtcg ttaaaatatt
     4381 ttctcgacgc tcctgtgcct tcatataaca aattccttct ttggtgttat ttcacaatta
     4441 aaaactggct agaatatcta accagttagc ctcaatcatt tcgtgcaaaa agacgaagaa
     4501 tatttaagaa tagattaata aaatcaagat aaaggcttag tgccatcgaa actgcccaac
     4561 catctgcgac ttggccgcct tggctattgt agacatattt aatcatttgg ttatcgtaag
     4621 caatcaatcc agaaaagatg atgacacata cgatactcat gatataactc atacccccac
     4681 taccgataaa taaattaaca agacttgcaa ttaaaatccc aattaaagca gccattaacg
     4741 ctttacgcat acctgataaa tcttttttag tcttagctcc aatcaaagcc attgcaaaga
     4801 aaacagccgc agatgttata aaagcttgaa gaactgtagt ttgcgtgtac cttgcgataa
     4861 tgaaacttaa agtaaaccca ttcaaagctg aataaatcaa aaataatggt aatgcagctg
     4921 gtgtgtttcg acgcgccgct ccactagcaa caaagactaa aaccaattct aagatgattg
     4981 ctccaaaata caaccctggc attttttgca tgatggcaat catattttgt ggaaacatat
     5041 acagcataat cgcagaaaca gcagctgaca aacctactcc tatccctaca agaccgtaaa
     5101 tcttagcaaa aaattgattc agccctgaat cagattgtgt gtaaatgacg ttatcattca
     5161 tatgtatttt cctattctaa attttaatga ttatgactac ctaagaattt gcgactaaaa
     5221 cggataggtt tcttaaaaat acgtttgagt ggacgagaag cctctcgtac tgcccaatac
     5281 ttatcaacca tagtcacaat ataactttct ttataacgtg gcggtgcaat agtcgctccc
     5341 cacatgtgct tcaaaataat atcttcttct ctagcgttta aatcagttaa ttttcgcgca
     5401 ttacgaaccg ctatccttgg atgaacccag gcatggctct tgttaaattt cgttacacgc
     5461 caatcataat aaaagaaatc atgtagtaaa ccacctcgag cagtactact cgcatcccaa
     5521 ccaaacttct tagcaatttt ataacttgta taactaacat ttatagaatg ctctaagcga
     5581 gttgagtgat gatgttggac aatagcttcg agtttttgga aacgtggatg gtcaataagg
     5641 tgaccgacca aatccatgaa gtccttatcg tcttgatact ttttcattat aatcacccct
     5701 ctggggatat tataacatat gaacttaaat taagctgaaa atgactactc ccgcagctac
     5761 tgctacatta agactttctg cttgtccagg catttcaata tgtgctaaaa catctgcttc
     5821 ttctgtcata gttttggaaa ttccctgtcc ctcatttccc atgattaaag caaaataatc
     5881 atatttaggg agattcttat aatttaccga ttgttcagat aaagttgtcg caatcatagt
     5941 tatcccttct gctttacaaa agttaacaaa ctgctctatt tctaccctaa atactggtaa
     6001 atgaaagtga ctgccttgca tagaacgaag tgttttttgg ttatacaaat cagcagattt
     6061 ctgagataaa aaaacagcat caaaattcgc tgcatctgca gttcgaacca ttgttcctag
     6121 gtttcctgga tcctgcacat cttctaatac aagaaaacgt ccttttttaa tatctgtcca
     6181 tcttgtctct tgaaaggcaa tctcagctac aatcccttga ggagtctgtg agtcagttaa
     6241 ttctttcaaa acatcatctg taacaacaat agctctctcg ggttttctca aacgatctat
     6301 tgccgtttct gtcacaaaaa tatttaaaaa ttgtgcacca tatttttcag cttcctcaaa
     6361 taaatgccag ccttcaataa gatatgaaga tttccgatat tttttttgta acaacttttt
     6421 cgttttttta atgagattat ttgattttga agttataata gtagtcataa acctattata
     6481 tcagaaaaga ggtcaagtta tgaaaaaagt tcatttaatt gtctcagggc gcgttcaagg
     6541 agtcggattt cgctatgcaa cttattcctt agctttagaa ataggtgata tttacggtcg
     6601 cgtttggaat aacgatgatg gaactgttga aattttagca caatccacag attcaaataa
     6661 aatgacacaa tttattcaga aaattcgaaa aggaccttca aaatggtcta aagtaactta
     6721 tgttgatatt aaactagata acttcgatga ttttaatgac tttaaaatga gtaattaaaa
     6781 atcattctgc ggaagtattg tttaattctt aaaaaaaaag taaaatagat aggtatatta
     6841 aaaattagag gaactaaaaa cattgaaaaa aacattgaaa cgtatcttat tctctagctt
     6901 atctttgtct atgttattat tattgacagg atgtgttagc gttgataagg ctggaaaacc
     6961 ctatggagtt atctggaata ctcttggagt gcctatggcc aacctcatta cttatttcgc
     7021 tcaacaccaa gggcttggtt ttggtgtcgc tatcattatt gtaaccgtca tcgtacgtgt
     7081 cgttatctta ccacttggac tttatcagtc ttggaaagct agctatcaag ctgaaaaaat
     7141 ggcgtacttt aagcctttat ttgaacctat taatgaacga ctacgcaatg ctaaaacaca
     7201 agaagaaaaa ttagcagctc agacagaatt aatgacagct caacgtgaga atggccttag
     7261 catgtttggc ggtattggct gcttgccatt actcattcag atgccatttt tctcagctat
     7321 tttctttgct gcacgctata ctcctggagt ctctagtgca accttcttgg gacttaactt
     7381 aggtcaaaag agtctgatac ttactgtcat tatcgccatt ctttattttg tacaatcttg
     7441 gttgtcaatg caaggtgttc cagatgaaca acgtcagcaa atgaaaacaa tgatgtatct
     7501 gatgccaata atgatggtat tcatgtctat tagtttacct gctagtgttg cgctttattg
     7561 gtttattggt ggtattttca gtattatcca gcagctagtt acgacttatg tacttaagcc
     7621 taaactacgc cgaaaagtag aagaagaata tactaaaaat cctcctaaag cctacaaagc
     7681 taataacgca cgaaaagatg ttacaaattc aactaaagcc actgaatcta atcaagcaat
     7741 tattacatct aaaaaaacaa atcgtaacgc cggaaaacaa aaaagacggg gataaaacaa
     7801 gcatactaaa taatggtgtg ttgtcctaaa cagttaaaga ttattatgta attcaagtaa
     7861 aaaagactta gttctatgtc ctacatagct aagtcttttt agttttgcct aacttttcat
     7921 ataaaaagca ccctaaaagt cagataaaaa tctagctttt aaagtgcaag taatctagct
     7981 aacagactac cttattaata aagtaatcgt ttttcccacc agtcttgaat aattgcataa
     8041 agcgtacaaa ttacccaata gataagggct actaagatat aggcggtcat atagtcggag
     8101 taggttcccc caactatttt agcattatta aagatatcag gtaccgtaat catcgcagtc
     8161 aatgaggaac ccttgaccat atctagcaaa acattactta aagaagggag tgcaattctt
     8221 gttgcttgcg gaataataat gcctctataa atcgtcggtg tttttaaccc taaagctcta
     8281 gcagcttccc actgaccatg atcaacagct aaaagagctg ctctgataat ttctgatata
     8341 tatgcactag acatcatggt aaaacctacg atagaagcag tcacagcatc taactgtatc
     8401 cctataaaag gcaaaccaaa gtagataaga aagagtagaa ccattaaagg aatcccacgc
     8461 attaaggaaa tatgaatatt agctagatac ctcagtaagc ggaaatgaga catcttcatt
     8521 agagctacaa agaatcctac gatagctcct aaaataaagc caaccaagga taatccaatg
     8581 gtatatggta acccctgaat aacacttgga aaagccttaa cagctagttc caaattaaaa
     8641 atggcatccc agtttatcac ttatctactc ctctcgaaat tttttattta actttaatta
     8701 ctggaatttt atccgttcca gcaggttctt ttgtgagatc ttgaccagca taatattttt
     8761 cagagatttt tttcaaagaa ccgtctttct tcatatcctt gatagctgca tcaatttttg
     8821 ttttgaggct cttatctttt ttactcatca caataccttg ctcagtcgga ttatacttca
     8881 catcacccat ttttactttg atatctggat attgttttgt tacatatttt acagcaatga
     8941 cttgtgtgta gtaatcattc ggaatgaaat ctgtacgtcc agttgataca tctcgaaggt
     9001 aaacgtcatt agttacattg tcataaataa caggttcagc accttgctgt tttgcaattt
     9061 tcatatattg cgttccagcg ccaccaccag cttttttacc tttccagtca gataaatctt
     9121 ttgctgtaat ctttgaagaa ccatctgcac gtacaatcat acctccaaca gaatatttat
     9181 aaggttgaga gaaattgtat tttttcaaac gctcgggagt tgtgtcaaag ttattgacag
     9241 caacatccac cttaccacta tctacagaag taaatgattc agcaactcca acttcaacaa
     9301 acttaacttt tatcttaagt tttttagcaa tcgctttcat catatcaatc tcataaccag
     9361 tcaatttttt atggtcatca taataacttg ttggataaag cgttgcaggg gtagcaacct
     9421 taaggacacc atctttttta atattgtccc atgttgtttc tttgttagat tgaccatttg
     9481 agcaagctgc taatgtcatt gcagataaac cgactaaacc aagtaaaatt gtctttttca
     9541 taagtactcc tgtagattaa attatattaa aatggtttgg cgtactctat cgaaaattaa
     9601 atttaatgaa aattaataca taaatttacg ataataatac aaccattgtt aagagtacca
     9661 tattgtatcc gttttcgcaa aaatattgtt tacttttcaa attaatggta aacaggccct
     9721 ttgaattgct tctctttttc aaacaactct gcaacagcaa gcaataaatc ttctctccct
     9781 ttagccgctg tcaattgtac acctaaaggt agcccctctt tagttagatg agttggtatg
     9841 gctaaagaag gttgccctgt caagtttggc tgatgtgtaa aaggtgtcca ggccattgag
     9901 tcttcaaaca ttttccaaat taaatcctgt tgttcactta ctgagaattc tcccatatgc
     9961 ctcaattgtt tctggagtgt ctcgtctaaa tcaaattgtc catgaaaagg agctggttga
    10021 ttagttgctg ctgttaaaat taaatcataa ttttcatgaa acctcgccat ggtagctgca
    10081 aattgatccc atgaatctaa gagtttactg taatctttag ccaataccct ttgtccactc
    10141 tgatacatag cccaggtcat taattccata tcgctaaatt ccattgaacg ccctaaactc
    10201 ttttcgatgt catcaaacat tgctgctgtt tcaacgctat tcatcataca ataagttttc
    10261 atagaatgaa taccatctaa aggttgttct actaattcta aatgattccc tttttgagac
    10321 agaaaatctg cggcttcttt aatagctgct ttagcctcac ttgatacctt tgtttttaag
    10381 ggagaatcca tcaatactgc tatttttagg ggtttactaa cgctaaactc gaaaagactc
    10441 tctttgctga gttttttcag gggaaatggg ctttctactt gatatgattg taaataatat
    10501 aatagtcgtt ttgtatctct aactgacttt gttaaagcaa aatgactaga agccccttgc
    10561 caacctctat aggatgaagg tccaactggt atacgtcctc gactcggttt taaaccaatc
    10621 aaaccattaa aacttgctgg aattctgatt gaccctcctc catcactagc tcctgcaatt
    10681 ggaaccattc ctgaactaac agctgcggct gcaccaccac tagagcctcc agcatttctt
    10741 gaatggtcaa atggaagatt aacattcccg tgaagttgac catcactaat attcttaaaa
    10801 ccaaattcag gggtatttgt tcttcctaaa ataataaacc ctaatttttc aaaggactgc
    10861 actaaatagt ccgtttgttt agcatgatag tgtttaaata attgtgaacc tgaggttgag
    10921 agttgtcctt tttgattctg tcccaaatct ttcagaagaa taggaactcc tgcaaaagga
    10981 gcatttgatt catttgcgta tttggctgct tgacgcgcct cattatattg cttgctgaca
    11041 acagcattaa cgcttacatt ctgttcctcg atcttgtaga ttgcttgctc tactaattct
    11101 tgcgacgaaa tcttatgctg ttttatcgct tgtaccatag cggtagcatc tttaaaaacc
    11161 atataaacac ctcataaaat gtatttttat tataagataa caaaaaatac ttaaaaaaaa
    11221 tagaataacg tctagactct agatattatt ctatgattta tactattaat ttgttttttc
    11281 aacactaatg atttcaactt gatagctacc agcaggtgat tcaattgtag ctaagtctcc
    11341 tgttttttta ccaattaaag catgagcaat tgggctttca ttcgaaattt ttcctgaaaa
    11401 aatgtcagct ccagcagcac caacgatatg gtaagtatct ttatcatttg taccaacttc
    11461 ttggacaagg acagttttac caattgcaac ctcatcttta gcaactgcat cactatcaat
    11521 aatttcagca taacgaattt ttgtttctaa aatttgaatt tgaccctcaa caaatgcttg
    11581 ttcgtctttt gctgcatcat attcgctatt ttctgataag tcaccataag aacgagcaat
    11641 cttgatacgc tctacaactt ctggtctacg aactaatttt aactcttcaa gttctttttc
    11701 aagttgatct ttttccactt gtgtcattgg gtaagttttt tctgccattt tatttctctt
    11761 ttcttttcat tttttcaagt caaagatata aacatgatga ctcgcaccat gtttataagc
    11821 ttagttaatt tgactattga tatatttttc aacgttagcc ttgtgtgttt caaaatcttt
    11881 agcgtagtat acatttccgg tcttcacatc agcgacaaaa taaaggtaat ctgttgaagc
    11941 aggttttata gtcgcttcaa ttgctgagat acttggacta tcaactggtc caggcatcaa
    12001 accagtatta gtatagatgt tgtatggtga tttaattgaa gtattaattt gagcatcttc
    12061 agctaaactt gttttatcac ctaatttccc catagcatac aaaattgcaa tgttactttg
    12121 aagagcttga ccggctgata atcggttata aaagacactt gctattttac ggcgatcttc
    12181 gtctgttgat ccctcttttt caacaagaga tgataatgtc aaaacatcat ttacagacat
    12241 attcttcgct ttaatagtgt tatagtaagg tgctatctta gtattcattg ttgaaatcat
    12301 atcttcaaca agtccttcta aggttgtatc tttataatag ttgtaggtag ctgggaataa
    12361 atatccttcc aattgataaa tagcttttga tttatcaggt aaacttccta gtaaatttgg
    12421 atatttagct accatttttt taataaacgt ttcatcttga acaagtttca agaaatcatc
    12481 agctttataa ggtgtacttg tagtctttgt atcaatttta ttagactcaa tagctttagc
    12541 aatctgttta atagtgtaac cctcggtaat taatatctta cctaaagctg gttttgtcgg
    12601 ttctgcagtt ccgccttttt ccaattcagc agcaatctga tctaaagtca tacttttttt
    12661 aagattgtag tatccacttt gaaaattact atagtttttg aacttggaat agtaattaaa
    12721 gactgtggcg ctcttaatga ctcctgcttt ttctaaaatt tgaccaatta atttattccc
    12781 agatcctatc ggaatttcaa cttgtacaaa tttatttgaa ttggaatcaa caggtttaat
    12841 agcactatct acaaaacgat aacctacaaa tgctgtcaag agcaatacaa gtatgataat
    12901 tgatatcaga atacgtgcaa ttcgttttgc cacattatct gctttctgac gtctttcacg
    12961 cttgttatgt ttgttcttgg ctgacaaagc tataccctct tcttcttttg acaaatcctc
    13021 tactcggata ggttcctttg ctacgggctc taactctgat gtctcaccaa gttttacatt
    13081 ttcaccttct gtaggaatat ctgttgctct cacaagaaag atttcttccg tttcttgatt
    13141 aggcacagca gttgcaacat cagtcttatc tgcttttaaa tctatgtcag gtacattttc
    13201 agttgtccta aattcactag atttggagcc gctatcaagc tctcttgaag gttccatccc
    13261 ttgattgata acttgttctt cttgggaagc taatgcttgt tccgcctgac gtctttcttc
    13321 ttctcgagca gcttctgcca gaagtttcgc ctcaatccga gccttctctt gctcttctaa
    13381 gcgttgctta gtctctagcg cctttgctct agcttcttgt tcgtctttca aacgttgcgc
    13441 ttcataatct gccatcaatt gtgctgttct gcgagcagct tctttggctt cttgctcttt
    13501 ttgatagagt tcttcttcac gcaactttct caaacggtta gcttcttcta actctgccag
    13561 aatttgttct ttaaaacttt tctgatcatg gttagaatgc tgatcgtcat taaattcggt
    13621 caaagagatt tcctcctaat aataatcaca ggcaattata tcttaaatag tctataaaat
    13681 caacaaaact ttgacaatgt tatctacttt taacaaattg atcgtgttgg attattccaa
    13741 atcatattat accaaagcga accaccatgt tgggagtcag attctccctc atctttaaat
    13801 ccattcattt catagaagga aattaaatca tcatggcaag ttaaacttat accatctcgt
    13861 tcttgagaaa caaccaggtc tttcatcgct gctaataatg ctgtaccaat cccctgacct
    13921 ttaaaatcag gatgtattga tagactagta attcctatga aaccacctac ccttacaggg
    13981 aattcagaaa ctttatggaa taaatcgtcc gttaagtacc gacctttaat aaccggtcct
    14041 tcaatgtaac ctgctaaacg accattaatc tctgcaacta aaaaagtatc agtcattatc
    14101 gttaaacgtt cttgcattgc ttttttactc gcagcctctg cttctgaaaa atttatttgt
    14161 tcaatactaa tgacttgctg taaatcctct aggcaaccat ttctaataat catactcatc
    14221 tccttaattt ctttatgaaa ttctcttgat aaagtcagat agaggactga gaaacttctc
    14281 tcagccctta ccatcaactt attgtgtatt tttagttagg ttagctaata attcttcaaa
    14341 agagcgctca tacaattgaa tgtctccagc acccataaag acatagacag cattatcatg
    14401 attgagtaaa ggcgagacat tttcgactgt cactaaatct gagtgtttga caatcttagc
    14461 agctaaatct tctaccttca cctcaccatt atctacttct ctagcagaac catatatttg
    14521 agcgagataa acgctatccg cttgactcaa ggcatgggca aattcgtcta aaagagctat
    14581 cgtacgagtg aacgtatgcg gttggaaaat agctacaatt tcttttgacg ggtatttttg
    14641 tcgagcagca tctaatgtcg caataatctc agtaggatgg tgagcaaagt catcaataat
    14701 gacagtatcg tcaataatct tctcagtaaa acgacgcttt acccctgaaa atgtcttcaa
    14761 atgctcagct actaatgcca tatcaattcc cattatgtaa aggttagcaa taacagcagt
    14821 tgcatttaag atattatgtt taccgtatgc tggtacatga aactgaccaa tttcttcttg
    14881 gttatagaaa accttaaagt cagaaccatt aacagttcga gtgatgtctt ttgctataaa
    14941 atcatttgaa tcttcaaaac cataataata tattggtgcc tcagaagtga tttcatgaag
    15001 ttttggatct tctccataaa tgaataaacc tttttgaact tgcttagcat agtcattaaa
    15061 ggcattgaat acgtcctcta ggcctgtaaa ataatcagga tggtcaaaat caatattggt
    15121 aataattgag tattctggat ggtacggcat aaaatgacgt tcgtattcat cagcttcaaa
    15181 cacaaagtaa ttagcattag cagaaccacg tcctgtacca tctccaatta ggaaagaagt
    15241 gtctgtaata ttttttaaaa catgagctaa taaacctgtc gttgaggttt ttccatgtgc
    15301 cccagctaca cctagactag tgaactgacg cataaaatct ccgagaaatt catgatatcg
    15361 tttaaaatga tagccctttt caataacata agccaactct tcattgttat ctggacgaaa
    15421 agcatttcct gcaataatct ctaaatcctc actgatatta ttcggtgaga aaggtaatat
    15481 agttacacct gcttgctcta aaccacgttg ggtaaaataa tatttgtcaa cgtcacttcc
    15541 ttggacgtta tgtcccattt gatgaagcat cagtgctagg gcactcattc cggatccttt
    15601 aataccaata aaatgataag tttttgacat ggtctctcct taattaatgt ctttaaaacg
    15661 tgtcagattt aattcttgag ccttggctct ttctttatga aactgattgt cttgtttatt
    15721 gtaaacttga ctgcgtttca aaaaatcaaa attatttttc tttgtttttg ggttcggtgt
    15781 attatcaggt tcttgaaata tgattggaat ttctgctaat atatagcttt cctgctgtaa
    15841 acgatcagaa agatgactta gttcagtcac ctttcgctga gtttgttttt tcggtactgt
    15901 tgccttcaat tgttgctgaa aagcttcctt tgatggcgcc ttaaattcct gtttcgcata
    15961 gtaagcttgt cgtttctccc gaacagacct ctttgcctct tgtcgagatt tgctcaactc
    16021 tgtcgctttt tttacaaaac tctgcggtgg tacaggatca ttttcaatta ccttaggatg
    16081 tgatatttct tcatgttcat aattgctttt gaccatgctt tgatatgttt tttcttgata
    16141 aaagcctcga atgttgttaa ttaaatcttc attatcataa agattcatct ttacaagcgg
    16201 agacattact ggttcatcat ctgctatcaa cggaaatcgt ctagagcctc tattcacatt
    16261 atcacttcct ttctacaaca gacctattat aaactaattt ttctgttttt tcaaagtttt
    16321 caactaagta aagcttatca gattggtatt tttcattcat ttatataaaa aagaaagcga
    16381 gagaccactt tcttttttat attatagtca catcatcata tttatttaga aactccaagt
    16441 atctctcgaa tatcatctac gctcatgcta gcatgggttt catttccatc cagaactgtt
    16501 gtaaccaaat gttttttagt ttcttgcatt tctagaattt tttcttcgat tgtgcctcta
    16561 gtaatcaagc gataaacctc gacattctct ttttgcccca acctatgtgc gcgactaatc
    16621 gcctgcattt caacagctgg attccaccac aagtcaatca gaacaacggt atcagctcct
    16681 gtaaggttga gaccaacgcc accagctttt aaagaaatca agaaagcatc ttttgatcct
    16741 gcattaaatg ctctggtcat ttcatgacgt tcatttgctg gtgtagaacc agtaatttta
    16801 taagttgtaa gccccatagc taccatctca cgttctgcga tatctaacat acccctgaac
    16861 tgagaaaaaa tcagagcacg atgtccattt tccttaattt gtgttaaaag ttgacgtagg
    16921 ctttctaact taccgctttc accatcataa tccataaaaa gacgtggagt gtcacaaatt
    16981 tgacgtaatc gtgtgatgcc agataaaatt tcaattttac gacgatttaa gtcagcgtca
    17041 ctagaatgtt ggatactttc ttgaatttgg cgtaattgcg ccaaatagat aactttttga
    17101 ctgtctgtca tttcatttgg atagttcatt tcaatcaaat ccggtaattc tgggagaact
    17161 tcttcttttc gacgtctcat cacaaatggt ttgatataac gagcaacttg tttagggttc
    17221 aacttcaaaa actctttctt accaggtaat aaaccaggta aaatgatctg gaaaatggac
    17281 caaatttcta acagtttatt ctcaataggt gttcccgata atgcaaaaca gtttttgacc
    17341 tcaaaagatc tcaagctatg ggctatttta gtttgagcat ttttcataac ctgtgcttca
    17401 tccaaaatta aataatcata atttccttca gaataagtct caaaatcctg cctaaatgat
    17461 gaataacttg taatgattac ttgatgacgt tcagcaataa tttcatctct acgaatctta
    17521 gaaccatagg caactgctac atctacgtct ggcgcaaatt tgtggaattc atcttgccaa
    17581 ttataaatta aacttgatgg tgataaaata agcacacgag aatctctagt tagttttgta
    17641 cttaaaaatg aaatcgtttg aagtgttttt ccaagcccca tatcatcagc taaaatccct
    17701 gcaaagccat aatgatctaa catacttaac caacggacac ctgtaagctg ataatcgcgc
    17761 aaggaagctg aaacagatag accaggtatt ttaaactttt ctgggtgtct caaatcttca
    17821 atcaattggt aaaaatgttg tgaaaagcta acattatctt gatttgcaaa cagttctgat
    17881 aattgaaatg ctgcaatatt atctaattca atatgcccat tctttgctcg tctagctctc
    17941 aacccttgca aagttgcact tactttctta gtttcttcat caaaaataac taattgccct
    18001 gatttattca caaaataagg attattgtca aacaaagcta ccagagcttg atcaacatcg
    18061 ttttccagta aatcagagaa atcaaaagaa atatctagca ggcttccctt agcttcaata
    18121 tcaatttgcg gtcgttctat gagacgataa ctttccaatt ttgcggataa gctcactgtt
    18181 cccatttttt taaattgtgg aagcatatag gtataaaaat cgtataattc ttgtgcactg
    18241 tatgctggat ggctggttga aaagtggggt gcaaagccat atttttctaa gagtttaaat
    18301 actttttctt catgtttgaa gtgactagca aatgttaact gttctagttc ttgacgatta
    18361 tgaacagtta aatcattacc ataatcaaat accatttgca ataaaatttc attttgacta
    18421 ttaatatcga actcaaaatt taccttaaaa tcatgaatag agaagctccg tggagcatcc
    18481 actagtccaa tctccttaaa atccagtaaa tgagctgcta acttatcttg atcatctaaa
    18541 tcaaaatgga tatgttttgc taaatttccc tcaatgggaa gactacgaat agccgttacc
    18601 atcttttctt gcttcaaatt aagatggtag aaagtatcgc gataaaacaa ataagaattc
    18661 gcaaatagtg gttttaagtc tttctctttg atttctaatt caattgattg cctatggaca
    18721 ataaccttaa attgataaag atgatcctca ccttctaatt ccttaaagta gaggtgatta
    18781 aattgatgaa aatcactttc aaatgaaaaa ttttctaaag cattcatcaa agtaacaccc
    18841 tcttcaaaaa aaccacgagt gagactaagg tgacgacctt gatttggtaa aataaactct
    18901 agatcaattt tgcttgaatg acttggaatg agcctccata aaaattctat caattcttga
    18961 ctggtctcat caaactgtat caaagacaaa gtttcaaagt attgttttcc aatctgataa
    19021 taagcttctt ttcgaacagt atttaaaaag gctttgatgt cacgaataac ataacttcta
    19081 tcatcaggca gtcgtctaat ttttaaagtc caccagatat cattagcata aggattttgt
    19141 tctccttgag ccgataattg atattttata gtatcatctt cattaataat tagactatct
    19201 aaaaaaacac taccaaaaga ggttttcttc ttagtttctt gttgactttc ttgttgaact
    19261 tgaactttag ataagattgc tttaccttct ggatcgtttt tcaagaaatg ctccagagca
    19321 gctaaatgtt gacaatagcc ttttcttgca aaaaaatcac aagaacattt tgtttcttct
    19381 gttactaatg aatattcaat ctgacaatcc ccaactttag cttttaatag attaccttct
    19441 tgagaaatca aggacaccaa accttgttcg tagagttcta taccttgatt tcgaatacgt
    19501 cctggaatca ttctactcat tatgctacca ccttcatctt atcccttaat tataacaaaa
    19561 ttcaagtcca atttcttgta aaaatactaa attgattgtt ttttgataca gtaacaaata
    19621 aaaaaggcta aaacaactgt cctaaccttc tatttcaatt tcaacaataa gatgttattt
    19681 acgtttacgt gctattagat taattggtgt tccttcaaaa acaaatgctt cacggatttg
    19741 attttctaag aaacgaagat atgaaaagtg catcaattct tcttcgttaa caaaaacaac
    19801 gaaagttggt ggtttaacag caacttgagt tgcgtagaaa atcttaagtc gttttccttt
    19861 atcagttggt gttggattaa tagcaatagc atccataatc acatcattta atacagcaga
    19921 cggaatacgt ttgttttgac tttcgctaat acgtttaatc atatctggta acttgtgcaa
    19981 acgttgcttt gtctctgcag aaacaaagat aattggcgca taagacaaga attggaagtt
    20041 atcacgtata tcagcttccc actgactgac agtatgattg tctttttcga tagtatccca
    20101 tttattaact acaataataa tacctttacc agtttcatgt gcaaaccctg caatacgctt
    20161 gtcatactca cgaatacctt cctctgcatt aatcaccata agaacaacat ctgagcggtc
    20221 gatggcgcgc atagaacgca taacagaata tttctctgtg ttttcatata ccttaccaga
    20281 tttacgcata ccagcagtat caatcatagt atactcttgc ccctggctat caacgaaatt
    20341 agtatcaata gcatcgcgcg tagtaccagc aactgggcta gcaataacac ggtcttcccc
    20401 aaggatggca ttaattaaac tagatttccc aacatttgga cgtccaatca aactaaaacg
    20461 aataatatct ggattttcat tttcctcttc taccggaaga ttttccacaa tagcatccaa
    20521 tatatcacca gtaccaatac catgaactga tgacaaggga taaggatccc ctaaaccaag
    20581 agagtagaaa tcgtaaatat cattgcgcat ctcaggatta tctaccttat taacagctaa
    20641 aataaccggt ttattggtct tataaagaat acgtgagaca tactcatctg catcagtcac
    20701 accttcttta cccgacacaa caaacacaat aacatcagcc tctgtcatag caatgtctgc
    20761 ttggtgctta atttgctcca taaacggtgc atcaacatca tcaatcccac cagtatcaat
    20821 taatgaaaac ttacgattta gccactcacc cgttgtatag atacgatcac gagtgactcc
    20881 ttcaacgtcc tcaacaatgg aaatacgttc tcctgcaatt ctgttaaata atgttgattt
    20941 tccaacattc ggacgaccaa caatagcaac tgtaggtaat accataattc tcctctcact
    21001 attgggaata ctcccaattc cattctattt tattaacggc gattcggtcc ttctaagtgg
    21061 aattctatcg ctaaataccg tacgcgctcc ataactcgtt ttgcttgcca agtttcgtcg
    21121 cttcctttta tattagccca ttttctttcc aaatcgttaa aactataatt tgacgtaaag
    21181 aaagtgggca actcttctag catacgatgt tgtaatataa cttgtaaaat ctcgtcacga
    21241 acccaggacg ttgcctgttc agccccaata tcatctaaaa tcaaaatcgg aacacttttc
    21301 acagcatcaa tttcatcttt tacagttccg cttgaaatag catttttgac atcaatagca
    21361 aaactaggga aatgaagaag agtcgttgac acaccctttc tctctgataa ttccctcgcc
    21421 attgctgcca ttaaatagga tttcccaacc cccatatctc catataaata aagccccttg
    21481 tgattatatg aaggataagt ctctacaaaa tcaagcagtt gtgacatagc tttcattcga
    21541 ctctcgttat taatatcaaa gtcagtcatt ctgatattcc gataggactt gggtagatta
    21601 actaaattaa tacgatcact gatagcttgt tttttttgtg cttcaattaa ctctcgtgtt
    21661 tctaaataag aaacatctgc ataaccttca ttcataacca aaataggctc atagccttta
    21721 gcaatatatt gactatcctt atttttaaat ttctgacgct caatcaaaaa ttgattaaat
    21781 ttagacatgc tgatatttat ttcacgctga gaaagttggt gggttttaat gaactccgct
    21841 acctgagcat ctgctaaaat catttgaata agctcatcat ttgtattacg aggcactctc
    21901 ccttgatttt ctaatgcttg tcccactgat ttcatgattc accatccttt cctaatcgtt
    21961 ctaaacgctt gagggcatct gttttaaact gttctaattc aatttctttt tctgggctaa
    22021 cctcatcttt ataatcagga ttactccatt caggaacatt cgattgtttc ttcttcgttt
    22081 ctgttttagt ccttactttt tgatctgaaa aatcacgaat tttaagcaca gcatcttcgg
    22141 ccgttataac attctgaaag gcaaaatcat tagcaacttt catgatatag gccttattta
    22201 agttagcaga ttttgtctta ttcaaggtat ataaaaccat aacattaatg acttcgtcta
    22261 ggaaattcat ttttgccaaa tcctcaagta aagtcttctc accacttgtt gtcactgcct
    22321 tacggcttct cttgattttt tcaaggaaca ctagagcact atcattttta gattccctta
    22381 gtatgacctt ttcattattt gtaaaactct gattatcttt aatatgctgc ccctcatttt
    22441 gttgaagttt cattcgctga ggcgcaatca ttccattgat agctgtttgt tttgctaatt
    22501 gataggtatc ataccaattt aaatgataaa gttctgatac actataaata cctaagacat
    22561 catctttttc attgttaaaa cgtaagccat ctcgcatcat caaacgtttg aaactttcca
    22621 agtcaaaaac atttttactc cggtttacct cttgttttgg ttgtcctaaa tccgagaaaa
    22681 catctgtaaa ttttttagag atatctctag catttttagg tattttcata tctaattctg
    22741 ccactgctac ctctcctata cgtttttcta acaaacgacg atagatggga ttagataaaa
    22801 ataactcatt tgacatagct tgattcaact taacaaggta agtacctgtg gcttggtaaa
    22861 cagtcaacaa atccaaagct gttaacatta ccaatgcatc ttgaaatctt ggcatcccgt
    22921 attgcagatg attgagaacc tctgaaaatt tatgacgtcg tattccgtca tcaaaaaaat
    22981 gaacaaaata atcatagagt gccatggcat cacttcccat aattgggaaa tacaattgta
    23041 ttaaactatt actatcataa acaatcttat tttgcttaat atatgtaaac tcatctattg
    23101 gactcatcac acgctaccac tctttttact ccgtacgcgc tttgtaattt gttgaagtag
    23161 ttcttcaatt tcatctacat ctttaaaaga tttataaaca ctagcaaagc gcacataagt
    23221 aatctcatct aattcggcga gttcatccat cactaaattt ccgatagtga tactagaaac
    23281 ctcatcttca tactcactcc taattttacg ttcgatacgt aagatacaat tttcaatatc
    23341 ttcgctcgaa acagggcgtt tttgagcact ctgaataata ccattcaaaa ttttatctcg
    23401 agaaaattgc tcgcgagtac catctttctt tatgactaaa agaggcaatt cttccaaacg
    23461 ttcaaatgtt gtaaagcggt tgccacattt ttcgcactct ctccggcgtc tgatagtcgt
    23521 accttcttca gcttgtcggc tatcaaccac actagattta ttatagccac attttggaca
    23581 acgcatgtca acacccctat tctatcatat catttaatca tcttatttta acacaaattc
    23641 agtaataaca aaagaagcgc ctctcttatc acctcatcat atcaaaacac aaaataatcc
    23701 ttatgccttt tatcattaag cataaggatt tttcttatat ttctttagtt tcttcaaatt
    23761 tttttatagg aatgcgaaca atgaattcag tcccttgatt tagttcactt ttaatatcca
    23821 cctttaaatg gtaagcatcc ataatttgtt taaacactga aagcccgata cctaaaccag
    23881 cttgtgtact ttctctgtta cgtgatttat cagttcgata aaaacgatca aagataaact
    23941 caatatcttc atctgagata ccttctcctt tatcttttac aactactgtc gcaaaatcat
    24001 tgtctactga taaaacaaca ctgacttctt tataactagg agagtacttg atagcgttat
    24061 caattaagat catgagagct tgctcaaaat gtatcttata tatctttccc caaacgatat
    24121 ctgaaatatt attttcaaaa ataaattgaa aatcttctct caaaatacgg aaattttcaa
    24181 taacaactga tatagagctt gataaatctg tcacttcatc ttgatgaagt tccaagcttc
    24241 cttggacgcg aatcatattt aacatgtcat taatcattaa ggacatacga tctgcctcat
    24301 ggtatgccac tgctaaactt tcttcaagaa tttctggatc atctttcccc caacgttgta
    24361 ataaaccaat atgacccttt acaacagcga caggcgttct taattcatga ctaacatcac
    24421 taatgaattg tgattgtcga cgagtatagt cttccaactg atccaacatc ccatcaaaaa
    24481 tcctagacaa ttctccaatt tcgtcttctg aacggacctt acttctaatt tctaaattac
    24541 taggattttc agatatctga tgcattaagt cgtgtaaatt tttaaccgga cgaacaatac
    24601 gcttagtagc actattaata acgaccaagg ctaaaacagt ccctaaaact tccatcataa
    24661 tcaagaaaac aatgatgttt tgcctcatac tataataacg acctaaatta tagaaaattt
    24721 gaacgtatcc aagcaactgt cctgtttttt gagagtagat tgcttttgta ccaacaaaac
    24781 cttccatatc tgcaacttta gagatgataa cttttcccat ggatttacca atagttggca
    24841 tacccgttct cagtgtagaa aaaactggtt tcctgttgac atcgtatagg gtcatttctt
    24901 gattaacata aagaatacta tcgattgact cgcccgttct aacaattcca ttttgacgct
    24961 tatgaggata gatgtcatcc gcaaaaacct ggttgtacag gatctctaaa atatcatcgg
    25021 atgtcaaact agagttcgct tgcgacaaac gaactctagt tttttctaat gacctaccga
    25081 cattaatttt ttccctgtga aggacgtatc ttgttgccga agtataaact aaaacagaaa
    25141 atactgtaaa taggctaaaa aataagataa aaactaattg cgataatttt tttgacagag
    25201 gttgttttac ccctataaac tgatcttttt tatttttcac gaatcacata gcccattccg
    25261 cgaacagttt gaatatatga ttcacggcct gggatatcaa ttttacctct taggtaacgg
    25321 atataaacat caacaacgtt tgtctctgct gccacatcgt atttccaaac atgttctagc
    25381 aactcttctc gtgtcataac acgattcata tttgtcatca agacattcaa caaatcaaat
    25441 tcacgttttg ttagtgaaat ttcctcatca ccacgcatcg ctgaacgatt atgagtattt
    25501 aatgacaaat cacggaaact accactatca cctttttctt ttgtcttcgt ttcaatttct
    25561 tggcgtcgga aaatcgctct aacacgtgct aataattctt cgattgcaaa cggcttaaca
    25621 atataatcat ctgctccacg atcaagacca gctacaatat ccataacaga atcacgtgct
    25681 gtcatcatca tgatataggt tgttttttca gcctgcaggc gacgtgtgat ttcgaaacca
    25741 tccatctctg gaagcattaa atccagtaga atcaaatcaa aatctttttc taatgctgtg
    25801 tccaatcctt cacgaccgtt tgtttcaaca acaacatcat atccttcatg tagtagttct
    25861 aacgagacga agcgagctaa atttttctca tcttcgatta ttaagatctt tttacccatc
    25921 tacaccaacc ttcttattct ttacttaaca ctatcaatta ttttttagaa tatctagata
    25981 aaaaacaaga tagcccacat tttactaacg tttctttaaa taaataaatc gccatcccaa
    26041 acaactgttc gatagcgaga cttaattttt attattgagt agtgtcctct gtcacttttc
    26101 aattctacta aaaaaaaatt aaaatatcaa ctgatgttat taataatttt tatctaatca
    26161 ctatcaaaca taccttctaa ggctgcgaaa ggattagatt tttctttttc tttagcttgt
    26221 ttttcttcat actgcttttc agtcattaac gaccaatttt tacctgataa atccgcttct
    26281 accccaacct catcagctgc cagaacacga agaggtatat ttaacaagat attatcgata
    26341 acgctttctt ctaaattaat atcatcttcc tctaatatta agaccaactc ctgatcaacg
    26401 agctcttttt tagtgctaac attttcagct tcaataaaaa cttcattaat agtttgcttt
    26461 tcagataaaa caactggttt catagaacga ctagacggta atgtaatatc gtaagccata
    26521 ttatagtcaa gtagatagag accatcttca tacaccacat gacctgaaac ttgaatatca
    26581 gaaatatcca ttatctctga atgacgctcc ataagcgact ctttgatatc tattttttta
    26641 tcaaaataaa gcccctctgg tgattttttg atttctgtaa gtaacacgtg ccatctccct
    26701 ttatagtata tgtcatatta tatcaagttt tacaggcttt gtcttaaaaa aacaaaacgc
    26761 aaggcatagc taccctacgt tttgttaacg actacatatg acgcaaacgc tcaatacgat
    26821 ctgcaattgg tggatgcgta taaaataaag actttaaccc ttcttttttc gttgggtcat
    26881 tgatatagag agccgcacta gcatcatcta ctggatgccc cataggttca gaacgatcaa
    26941 gtttttctaa agcagagatc attccttgtg gatttcttgt taactcaaca gaactagcgt
    27001 ctgctaagta ttcgcgctgt cttgaaatag ctaattgaac taaactagcg gctaatggtg
    27061 caaggattag tgataaaata gagaaaatca aaaccaagat gtttccacca tcttctctgt
    27121 catcatcacg cctacgacct ccgccgtaaa ataacatacg gctaccaatg cttgatatta
    27181 gagttacagc actcgctaaa gctactgcaa tggttgaaat acggatatca taattacgaa
    27241 tatgactaac ctcatgtcca ataacaccct ctagctcttc gcggttcata acagctaata
    27301 ggcctgtcgt tgctgcaaca gcagcatttt ctggcttaga gcctgtggca aaggcattta
    27361 gggagtcatc ttcaacaatg aaaacgcgtg gcataggtat ctgagctatc attgccatat
    27421 cttcaacaat atgaaaataa tttggcgcct catcttctgt aacttcacgc gcattgttca
    27481 ttgacataac aacattagtt gattgaaaaa tcatacttac tgcatagata accccaataa
    27541 ttagagctag aacaagccca aattggtagc tgcctaatac caaataacct acagctgctc
    27601 caataactgc taaaagacag aagaagacaa ttaacaagac aactgttttt ctcttattac
    27661 ttgcaatttg ttgatacagc aaaagcctct ctcccttctc aattaaaagt caaaggaaac
    27721 ttttggcact tctttttctt cttcaggagt ttctaagaat tggcttggtt tgaacccaaa
    27781 tagttttcca acaatattac ttgggaaagt ttctaatttc acattataat tggatgttgt
    27841 tgtgttatat aactgacgtg aataagaaat tttattttcc gtattagtta gttcttcttg
    27901 aagtttaaca aagctattgt tggcttttaa atctgggtaa ttttcagcaa ccgctaaaat
    27961 acttgaaagc tgtcttgtta gctcattact tgctgtcatt gcttcagcag gtgtgttagc
    28021 tttagcaact tgagcacgca gttctgcaat cttctcaagt gttttccctt cgtacgcagc
    28081 atagccttta acagtttcga ttaggtttgg aattaaatcg ttacgacgtt tcaattgaac
    28141 gtctatttga ctccatgatt ctttagtatg catgcgactt ctaactaaac tgttataagc
    28201 aacaatcaac caaataacaa acaaagcaat aattgctatt aaaatcatag ttcccatagt
    28261 agtagctcct ttacattttt tccattatag caattttctt tagattagga aagtcctgtt
    28321 attcccatta ttagtatttg tgataaaata gagtcattat acgatataga aattgaggtt
    28381 tactatgaca ccccaagcat tttatcaagt tctgatagag catggtatta cacttactga
    28441 caaacagaaa aaacaatttg aaacttattt tagacttctt gttgaatgga atgagaaaat
    28501 taacctaaca gctattacag ataaggaaga ggtttactta aagcacttct atgattctat
    28561 tgcgccgatt ttacaaggtt atattgacaa tagtcctctt agcattttag atattggtgc
    28621 tggtgcaggt tttcctagta ttcctatgaa aatcctttat cctgaaattg atattactat
    28681 tattgattca ctgaataaac gcattaattt tctaaacata ttagctaatg aacttgaact
    28741 ttcaggagtt cacttcttcc atgggcgcgc tgaggacttt ggtcaagata aagtatttcg
    28801 agctaaattt gatatcgtaa cagctcgtgc agtagctaga atgcaggttc tagcagaatt
    28861 gacaatacct tttcttaaag ttaatggtcg cttgattgca ctgaaagctg cagctgctga
    28921 agaggaatta atttctgcag aaaaagcttt gaaaacacta ttttcacaag taactgtaaa
    28981 taaaaattat aagttaccta atggtgatga ccgaaatatc acaatcgtta gcaagaaaaa
    29041 agaaacgcct aataaatacc ctcgtaaagc tggtactcca aacaagaagc ctttgtaaag
    29101 ttgggagcta gtatgaaaca tttctttgat tacaagacaa tgtctgtcgc acgtaaactc
    29161 agcattagtt ttatcgctgt tatcctattg ggaagtattt tactttcact tccaattttt
    29221 caatatgcta acgcccctaa aacacattac attgatcacc ttttcacgac tgtttcaatg
    29281 gtatgtgtaa ctggtttatc tgtttttcct atcagtaagg tttataacgg ttgggggcag
    29341 attgtagcta ttttactcat gcaaacaggg ggacttggtt tagtaacact aatgtcgttg
    29401 tcttactata ccctaagacg taaaatgtcg ttaaatgatc agaccctttt gcagtcagcg
    29461 ataacttata acagttcgac agatttaaaa aaatatctct atatgatttt taaagtaact
    29521 ttaactttag aagttttagc agccagcatt ttagccatag attttattcc tcgatttggt
    29581 ttgggtcatg gtatctttaa tagtatattc ttagctgtct cagccttttg taatgccggt
    29641 tttgataatc taggggcaac gagcttagct caatttaaac taaatccttt ggttaacatt
    29701 atcgtttgtt tcttgattat ttcaggagga cttggatttg ccgtatggaa agatttaatt
    29761 gaagctacta tacaaacatc tcacaaggaa ccaaaattaa tcaagacatt tccaaaacgc
    29821 ctcagcaacc attctaaact ggttttaaaa acaacaacca ttattttact aacaggaact
    29881 ttgctttctt ggttgttaga gtttggaaat tttagaacaa ttgctaattt atctctacct
    29941 aaacaactaa tggtcagctt tttccaaaca gttacgatgc gtaccgctgg attttcaact
    30001 attgattata cccaaactga tttcgcaacc aaccttgttt atattatcca aatgttaatt
    30061 ggaggagctc ctggtggaac ggcaggagga tttaaagtca ctgttatcgc cattttactc
    30121 ttactattta aagccgaact gagtggccaa agccaagtga cttttcatta tcgaacaatt
    30181 ccttccagta ttattaaaca aacactatcg attttaactt tcttttttat catacttatt
    30241 agcggctatc tccttcttct ggaactcaat ccccatatag atcctttttc tcttttcttt
    30301 gaggcatcta gtgccttagc aaccgtgggt gtaacaatga acactaccaa tcaattaact
    30361 ctgggcggtc gaattgtaat tatgttcctc atgtttattg gtcgagtcgg tccaattacc
    30421 gtactgctat caattcttca gaaaaaagag aaggaaattc actatgctga aacagaaatt
    30481 attctaggtt agaaaaggtc ttttatgaaa acaaaaatta ttggtgtact tggtctagga
    30541 atatttggac aaacactcgc acaagaacta agtaactttg aacaagatgt tattgctatt
    30601 gacagcaatc ctgaaaatgt acaagctgtc gccgaagttg ttacaaaagc agctatcgga
    30661 gacattactg atttagcttt cctaaaacac atcgggatca gtgactgtga tactgttatt
    30721 attgctacag gaaacagttt agagagctca gtattggccg taatgcactg taaaaagtta
    30781 ggcgtcccac aagttattgc taaagctcga aaccttgtat acgaagaagt actttatgaa
    30841 attggtgctg atttggttat ctctccggag cgagaatctg ggcaaaatgt tgctgcaaac
    30901 ctcatgagaa ataaaattac agatgtcttc cagattgaat ctgatatttc tgtcattgaa
    30961 tttaaaattc ccaaatcatg ggtagggaaa actgtcgaac aattaaatat ccgtcacaag
    31021 tttgatctta acctcatagg tatcagaaaa gcaaaaaaca aacctgtcga taccgaagtt
    31081 ccaatcaata ctccacttga agaaggtatt attctagtgg ctatcgctaa ctcagatgcc
    31141 ttccaacgct atgactattt aggatacttt aactaaagaa aataaaaaac tgactattaa
    31201 agtcagtttt ttattgccat gggttataga aacgtccatg attaaggtga attaaataaa
    31261 ggcttacaaa cagactagct agagcaagta tgattaagac aatgtcactt ttttccaaag
    31321 attgcttaga gtaccaagta cgtcttttat ttttaccaaa tcgtcgaagt tccatagcag
    31381 ttgctactgt atcaatacgt tctagagagc taaaaattaa tggtgtgaca atctgcaaat
    31441 tccccttaat gcgtgctact aagttcgatt tttttgataa ttctattcct cttgcctctt
    31501 gtgctcgacg tattgtataa aattcttctt gaacatcagg tatatatcta agggtcaaac
    31561 taacagcata agctatttta tatgataaac ctaattgatt taaacttgat gcgaactgac
    31621 taggattagt tgtcatcaaa aacaaaagag ctaaaggcac tgtacaaaag tattttaaaa
    31681 tcagattaaa gaggtaaaac aactcttggc tagtcacatc atatgggcca attcctttta
    31741 acaataaaga agaactacca taaatcttat caccataatg gggagcaaaa agatagacca
    31801 ttataatatt taaaacagcg aatacaacaa ctaaagtcat aacaaaggac acctgttgcc
    31861 atttgatttt tgctaatcta aataagacta gcgatcccaa tgaaacaaca attaaaaatc
    31921 ttgtatcata agtggtcatg caagcaacag aaactaaaat aaaaaatagt aacttactgg
    31981 cacctgatag cctgtataga aagccataac cctcttgata accaattaat tgtttagcca
    32041 ttcttacctc cttgattatc aatgtaccat tgtgttagtt tctgtggaga tatccctaat
    32101 ctgtctgcta aagcaaataa actcgttttc tttaaatgag tctttttcaa aagctctgta
    32161 tttgaaagga tgttaatagg atgatcatct gcaacaatca gaccattgtc gattacaatt
    32221 gcacgatctg tatactctaa cataagctgc atatcatgtg taatcataac aatggtatgt
    32281 ccatcacaac ttagtttatc aaggaaagac atcatttcag tataatgctt catatcttga
    32341 ccagctgttg gttcatccaa aataataact tctggattta aaatcagtat cgaagcaatc
    32401 gtaacacgtt ttttctgacc gaatgataga gccgatatag gccaattcct aaattggtag
    32461 agcccacaaa cctttaatat atcgtagaca cgactttcta tatcattatc actaaaacct
    32521 cttaaacgga gacccagagc aacttcatca aaaaccatcg cttgacttat catctgattg
    32581 ggattttgca aaacatagcc aattcgttct gctctttctt ttatagagtc ttgactgaca
    32641 tcttcattat tataaattag agtagcatta ttagggataa attgacataa cgctttagct
    32701 aaagtagatt tacctgcacc atttttccca acgatagcta accgctgacc tttatataaa
    32761 tcaaacgaaa catcatctaa agtattattc tctaaatcat atgacaccga caaatgctgt
    32821 actgataata atttatctgt atgtttatct aaatctaatg ctcgcatttt aggtatcaat
    32881 agttcactga aatcaaagtc atcaatagac gtcattgtat tttgtttttc aaagtcatat
    32941 cctaaatttt tgagagcaga aatatacagt ggttctcgaa taccatattc agataaaaaa
    33001 ttagttttta aaagttgatc cggctcccca ttataaataa tttgtccatc actaaaaaga
    33061 actacacgat ctactcgctc ataaagaaca tcttccaatc gatgttcaat aataatagtg
    33121 gtcgcatctt gctcttgatg aatacggtca accaatgcca tgatatcttg tcccgattgt
    33181 ggatctagat tagccaaagg ctcatcaaat aatagaattg ggctatcgtc tactaaaact
    33241 ccagctaaac taacccgttg tttttgtcct cccgacaagt cttgtggacg tttagacaaa
    33301 agaggagcta tttctaaacg ttttgcccac atttcaacga tagaggccat ttcttcctgt
    33361 gctacaacat cattttctaa tgcaaaagca atatcttctg ctactgtcaa tcctataaat
    33421 tggccatctg gatcttgtaa aaccgtagaa actaaatgag acttgtcata tattgacaag
    33481 tcaaaagcat ttttatggtt aatcgtaaaa ataccactat attggccttt atgagtattt
    33541 gggataatac catttaaaca atgaccaagc gttgacttcc ccgaccctga aggtcctaat
    33601 atcaagacct tttcaccttt aggaatgctt aagttaatcc cttttaaagt tggttcagat
    33661 tgaacgtcat attgaaaggt aaaatccttc cattcaataa aatctttcat agattactct
    33721 cttatcctaa tctttggata aactaccttt ttgtggtcgt gatttagcgt atgctagcaa
    33781 caaaagcgta ccacctactc caatagtaat ggaattcaca aggcttgata agaaaccttg
    33841 tgcaaaaact ttgcttgcag gttcgctata aaagaaaata tcaccatacg gtgcaacgac
    33901 agaccaacct ataaggttag caataacttg aactacatta aatagaagca aatccttctt
    33961 aatcatgcca gataattgaa tagctaattt tttagcaaaa aagcctatca tcaaacctaa
    34021 aagaccacta actaaaaccc aagtccacca cggagaacca tattgaatcg aatctttcaa
    34081 tgcgtgtccg ataaaaccag taaaaaaacc aactcctggt ccataaataa ctgcaaataa
    34141 tgctaaaact gcatactgca attgaatatt agtatttgga attggtgttg gtatattaac
    34201 tagcatacct ataatgataa aaagtgcagc tccaattcca gtcgctacaa ccttttttat
    34261 tgtgtttgta ttcataaaaa cctctctgaa aaacaagctt aaacagtcac ttacaatctg
    34321 ttttatttta tcatatttca agctgatttg aaaccttatt gacaagaaga actgaaaaac
    34381 gattgaaaat aatattaaat accaaaaaag atctcagata gaatacctaa gaccttcatt
    34441 agtttatttt tttccaaaaa ctgatttttc agctacagcc gtgaacagaa cgtctgtcga
    34501 tgaatttaaa gctgtctcac acgaatcctg tatcacccct acaatgaaac caactcctac
    34561 gacttgcatt gcaacgtcat ttgaaatacc aaataagcta catgccaccg gaatcaataa
    34621 aagtgaacca ccagttacac cagaagctcc acatgctgat acagcagcaa ccacacttaa
    34681 taagaatgct gttggaaaat caacagtaat tcctaaagta tttactgctg ctaaagtcag
    34741 gatattaatt gtaatagctg caccagccat attgatagct gcaccaagcg ggatagatac
    34801 tgagtaagtg tctttatcta atcctaaatc ctcacatagt ctcatattaa caggaatatt
    34861 cgccgcagaa ctacgtgtaa agaaagctgt aatacctgaa tcctttaagc atcgtaatac
    34921 caaagggtaa ggatttttac gcatcataac aaaagcaatg attgggttaa cgacaagagc
    34981 tacaaataac atcgtaccca ccaaaactaa tattaaaaga ccataatcac ctagtatacc
    35041 aataccgttt tctgaaatgc tagtgaaaac caaccccata ataccaattg gggccaaatt
    35101 gataatccat ttgacaactt gtgaagtagc atcagcaaaa gtttgcatta aatcttttgt
    35161 acgctgatta ctgcttcgca tcgccaaacc aaaaataacc gcccaaaata gaatactcat
    35221 ataattggcc tcggtaatgg catgaatggg attatcaaca attttcaaca aaagagattg
    35281 aaagacttct gaaatcccct tgggaggtaa taagtctgtc tttgctggcg ttttcaatat
    35341 cagtgtcaaa gggaaaatat agttcacaac aacagcgatt agggccgctg caaaagttgc
    35401 agtgatatac aaaccaatta tcgttgacat attggtctta ccgccctcac ggtgttgcga
    35461 caaggcacta ataactaaag tgaaaactaa aagaggggca atagctttca accctcctac
    35521 aaaaagttga ccaagtaaac caatcgcaga ggctttaggg actaaaatac ccaagaataa
    35581 gccaataacg actccaattg ttattttttt tatcaagctt gtttttctcc aagcttttaa
    35641 aaatcgtttc atactaaact cctaaaaaat tcttaaagtc tatgatatga taaaaaaaaa
    35701 gattcgtcaa ataccttttt tattttttag aggttgacga attctaaatt tttctgatat
    35761 aattactttt tgaaatattc aatcaatttc gaaaataaaa tatttttttg tacagaaagg
    35821 tacattgatg aaactaaaaa acacttatat cattatcggc ttgatgcttt ttgcgctgtt
    35881 ctttggagcc ggtaatctta tttatccagc ctacttaggt atctactctg gttctaattg
    35941 ggcgtgggct atcattgggt tctgcttaac aggcgtaact ttacccctac ttggtgttgc
    36001 agctgtgacg tactcaggag caaccgatgt tgaaagcttc accaaacctg tctctaaaac
    36061 atatgctatt ttctttgcag tagcactata cctatctatt ggacctttct ttgccattcc
    36121 tcgaacaggg gcaacctcat tctcagttgg tattcaacct atcttaggcg acagctcctt
    36181 agtcaaaatt atctatggtt taattttctt tggtatttct tatttcttag ctattagacc
    36241 tagtcagatt gtagatcgta ttggtaaata tctaactccc tttttactaa ctgttattag
    36301 tattttaatt attgcctcac tcatccatcc agctgctatc gttggaacac cacataacgc
    36361 ttcaacgaat gtcagcgatg ctttcaagga tttaccattc ctagcaggac tgattcaggg
    36421 gtatggtaca atggatgccc ttgcatcaat tgcttttgct attatcgtta ttcaagcaag
    36481 taaacagtac ggagcaatta ccaaaaaaga aattacttct atgacactta aatctggtgc
    36541 tatcgctaca tttcttttag cttttattta tatttttgta ggacgtattg gtgcaacttc
    36601 acaaagcttg tttaaatttg caaatggttc cttcttactt cacaatactc caatagacgg
    36661 tgggcatgtt cttagtcaat cagctaactt ctatttagga attgtgggac aagctatttt
    36721 aggtacggct atttttcttg cttgcttaac aacagctact ggattaatca ctgcttgtgc
    36781 ggaatacttc cacaaattac ttccaaagat tagtcacatt acttgggcta caatctttac
    36841 tttaattgct attacgttct attttggtgg tttatcagag attatccgtt ggtcactacc
    36901 agttctctac cttctctatc cattaactat agtcttaatt ttcttagtct tttttgatca
    36961 aaaatttgaa agtagccgca ttgtctacca aacttctatt gccgccactg ctgtcgctgc
    37021 tttatacgat gccttatcaa aactcggcga aatgactggt ttatttacca ttccatccgc
    37081 tttgaccaca ttctttacta aagttgttcc tttaggtgaa tattctatgg gatggattag
    37141 ttttgcaatt tgtggtgtct tagttggtct tattctaaaa aaagtaaaag cataataatt
    37201 taaagcagta cgaaaattac tgcttttatt ttaaaataat tattgtaacc gctttctttt
    37261 ggtggtataa tagccttgaa aataaataat ttttggagga actctaatat gagagctgca
    37321 acttatcttt cagctaatga attatcactt actgataagg ctaaacctca agtaataaaa
    37381 cccacagatg cggttgtaag acttgtcaaa acaaccattt gtggtactga tctacatatc
    37441 cttggtggcg atgttcctgc ttgtaaacca ggaacaactt taggtcatga aggcattggt
    37501 atcgtcgaag aagttggttc tgctgtcaat cattttaaag tcggagataa agtaattatt
    37561 tcttgtgtca ctgcctgtaa tacttgctat tactgtaagc accagttgcc ttcacattgt
    37621 gaagatggcg ggtggatttt aggacatctt attgaaggta ctcaagcaga atatgttcat
    37681 attccacatg cagatggtag cctttatcat gcacctgaag gtgtttgtga cgatgcattg
    37741 gttatgttat ctgatatttt accaacttca tacgaaatcg gcgtcttacc ttctcacatt
    37801 aaaccaggtg atactgtttg tattgtaggc gctggaccaa ttggactatc tgccttacta
    37861 actgcacaat tctattctcc tgccaaaatt attatggttg atttatctca aaaacgactt
    37921 gaagcctcta aaaaatttgg tgcaacacac accattctat caacgtctac tcaagaagtt
    37981 aaagaagaaa ttgataaaat taccaaaggc cgtggcgttg atgtcgtatt agaatgtgtt
    38041 ggttatcctg caacatttga tatttgtcaa aatgtagttt ctattggagg acatatcgca
    38101 aatgttggtg ttcatggtaa acctgttgaa tttaacctcc aagatttatg gataaaaaat
    38161 ataaccctta atactggatt agttaatgct aatacaactg aaatgctcct tgaagttctt
    38221 gagacaggca aaatcgatgc aacacaacta gtaacacatc acttcaagct atctgaaatc
    38281 gaagaagctt ataaggtatt taaagctgct gaagaaaaca atactctcaa agttatcatc
    38341 gaaaacgata ttactccagc ctgataattt ttaaacaaat gaaaatgtga gaagaatagc
    38401 cttctcacat ttttatttat gactcaagcg acgtgttaaa aaatcaccta aaaattggat
    38461 aaagaaaatt aataataaaa tcagaatagt cgctactaaa gtaatatcat cacgagaata
    38521 gttatatcct ttagtaatag caacagaacc caatcctcct gcaccaatag cgccagccat
    38581 agctgtttca cctactaaag aaatcaaagt aaccgttgat actcgaatta aatctggtag
    38641 accttcacga agataaacta caataatatc ccaaagtgtt ccacctgagg cttgtgcagc
    38701 ctcaataact ccaccatcaa gttcagctaa aacaacttga acttgacgag caaaaaatgg
    38761 gaaaactgcc aaagaaagag gtaccaaagc tgctggtgaa ccaagtgttg ttcctacaat
    38821 tacgcgagtt actggcgcaa tcaaagcaag aagaataatg aagggcagag ctctaaaaac
    38881 agaaacaact ttatctaaaa ctccaaatac taatttatta gcaataactc ctctaggact
    38941 agttaaaaca aggaataaac ctcctaacaa tcccattaaa cctccaataa ggaacgacca
    39001 aaaagtcata taaagggttt gtacaatagc tgtctgccag ccgtaagcac cttcccaacc
    39061 catttgatat acatttggta aatgtgtttg aatccactct atcatgcttt accaccttct
    39121 ttcaataacg ttaattgaac ttgcgaatct gtaattgctt ggcaggcttg tcttaatttt
    39181 tcttcgtcac ctgataagat aactaccatt tctccaactg gaataccatc taaaatttca
    39241 atattcccat agaggatgtt agcagttact tcaaattctt tataaatttg atttaaaagt
    39301 ggctcatccg tgctatgccc agcgtatttc agttgaacta atttgctacc tactggtaga
    39361 tttttgacaa cttcttgttg ctcaatctta agcattgcct catcaatccc agtagctatt
    39421 tttataaaat cttgtgtaag actctctcta ggatgtgaaa aaatgtctaa aactgatcct
    39481 tcttcaatca atttaccatt ttgcataaca gcaacgcgat tagcaatgtc tttgacaatt
    39541 tgcatctcat gtgtaatcaa aacaatcgta agtcccaatt ttttattcag atcttggaga
    39601 agggcaagta tttgttttgt tgttttaggg tcaagtgcag aggtagactc atctgaaatt
    39661 aagattttag gatcgttagc caatgcacgc gcaatggcaa cacgctgttt ttgccctcct
    39721 gataattgtg aaggatagtt ttgagcacga tcggataatc caacaagttc taataattta
    39781 gcaaccttag ctgctttcgc ctcttttgat aaacctgaat gtttaagagc aaaagctaca
    39841 ttctgttctg ctgtcaactg tgacattagg ttaaaatgtt gaaaaatcat tccaatttca
    39901 cggcgctgtt cgcgtaattg tgtgctagtc agtgtcacct tgttgtcgta aataacttgg
    39961 tcatcaatag taattttacc tgctgaaggt tcttgtagca aattaataac acgaacaagg
    40021 gtagattttc cggctcctga ataaccgaca attccatata tatcaccttg attaatatgg
    40081 atagtaacat ctttaacagc attaatctca cgtttctttt ggtggaaagt aacatcaatg
    40141 ttgtctaatt taataattgg ttctttactc atatgattct attaaagcct ccactaattc
    40201 aatatgggtg taataatcag caatactaac attttcatca acgccatggt ctcgactatt
    40261 ggcatgtcct atgccaatag ctgcaattgg cgctcttaga gcttgatgaa ctaaatacat
    40321 tggtcctgta cctggtgagg taggtaacag agatatccct tctggataaa attgctcagc
    40381 taaatcaaca acctttagga tagatggcgc tgacatatca ctacgataag acatttcacc
    40441 aagggtatag gttagctcaa tgtctttaaa gccattctct ttcaaatgat tttgtattga
    40501 atctagtaca cctttcgggg ttaaccctgg tactaaccta acctctgctt tacatttagc
    40561 ataagcaggt aaaattgttt tgacgccttc accttgataa cctgaggtta taccttcaat
    40621 ggctatagaa ggttcaaaat aaagctttct caaaaaagtt ttatgactat ctgctaaact
    40681 gggcaaaact aattggtaag caccttctag cgcctttgca gatctataag aatatttttc
    40741 aactaattct aactctctct tagtaggggg aataacttta tcataaatac cttctaccaa
    40801 tatatgacct ttgttatccc ttaacgtatt gagggcttga attaaatacc atgtcgagga
    40861 atcaacaacc ccaccaaagg atgaatgaat atcaacatcc gctgaactta ctgataaatc
    40921 aaaagtgaca atgcctttat ttccaccagc aatttccaac tgtcctttag gatttttagg
    40981 gccatcttcc cacacgatca aatcagcccc ttgtagctgc tcttgatact tttctaaata
    41041 ataatcaagg cctactgaag ctgattcctc tgctccttcc acaataaaag tgatatctag
    41101 aggaagttcc cctttgtgtc ttgataaata ttttttaact gctgataaac gtgcggtaat
    41161 gtgaccttta tcatcatcta caccacgtcc atacatctta ccatatcgaa gtgaaagtgt
    41221 aaaagggtcc tcagtccact gttctacctc atctgctggt acagtatcat aatgattgta
    41281 aaaaataata cgcttagcat ctacttttga tgatttgaaa tttgccacga taaagggagc
    41341 gctatagctg tcatctataa tgacttctgc gccagctttt ctgaaaactt ctccaagata
    41401 gcttgataag tcatttaaac caactttctg agcaaaaatt gacttaattg caattaaatc
    41461 tcttaaaata ttgatatcat cctgaacaat agtatcagat agaaactttt gaatttgatt
    41521 ttcttcgttt gttaaagtca tctttcatcc accttccttc aaaaacaatc caagagcctg
    41581 gcacattgta ttcgtcccag gccctttatg gctatatggc tgataagttg tatcctatta
    41641 ggattccctg ttaaacttat acagtcaatt cacgttacca ttgtggaata tcagctgaag
    41701 tatctttgat aacttttttc acttcatctg tgtgataagc atccaagata gcttggatag
    41761 ctttagcgtt cttttgcttt ttccaatttt tacgtcccgc aatgatatta atccattgtt
    41821 ttgaattttt atctgatttc tcaacaaaga tagcatctga aggttttaaa ttagcttgct
    41881 caatgtatgt attattaata atagctgcat ctacatcttt gagtgcacgt ggtgtttgac
    41941 tcgcatctaa ctcctgaata ttaatatcct ttttattaga tgtgatatta gcaactgttg
    42001 caaccttctt accagaaaca ttcaatttga ttaaacctgc tgactgaagg acatacaatg
    42061 cacggctacc atttgttgca tcatttggaa ttgcaatagt ggctcctttt ttcaattttt
    42121 taagagattt taccttctca gaatagatac gaattggagc taagtaagtc ttttcaagtg
    42181 gaattaagtt tttcttattt tccttattcc agttttctaa gaaattgtaa tgttgaaagg
    42241 cattaatatc cacatcctta ttggctgtcg cttgatttgg ttgtgtataa tctgtaaatt
    42301 ctgtaaattt gattttagct ttatcaccta ctagcttttc aattttatcc caacgtgctt
    42361 tttcagtgtc agaaaaggtc ataacaccaa ctttaacaac tttacttgaa gttgagcttg
    42421 ctgaaacttc ttgattacta aatgtgactc ctaaaagtgt tgttaaggct aatcctaaga
    42481 caatatgttt taacttcata ataaattccc tcaatttttg tgataaagat attatcccat
    42541 aggaagttat tagaatcaaa ttgttataaa ctgtctcttg ttatagtttt aaactatata
    42601 acaaaaaact aaggattgac atctccttag tttttattac tacttctcct taggagtaac
    42661 gagatctttc tcatttggta cgtaatcacc tccaaggtat tctttagcta tttttgaaag
    42721 tgtcccatct ttacgtaatt gtttaaggcg attattcaca tacttttgca agtctttttg
    42781 atcctgacca aagatgaaat aaatataagg ctgatctctc atagtcaaag ggatggtttt
    42841 taaattcgtg agaccttgat tctttatgat agcgttaaca gttggtccgt caaaaagttt
    42901 aaaatcagct tttccatcac tcaaattcgt tagaatctgt gtaatatttt cattagtata
    42961 ttttagagta acaggatttt tctgatgctc tttattgaaa ttttctaatt gcttggcagt
    43021 tgtcgttcct tggacaacct gtgttttatg accactaata tcattataag ctttgatatt
    43081 actattctta ggaactgcta aaactgaagg agtagagcct attgggtatg aaaataaata
    43141 tttttgagat ctctctgatg aataagaaat attatttcca cccatttgaa actttcctga
    43201 atcaatgccg gtaaataccg atgaccattc tgttttttta aaagtgactt tatagttatc
    43261 tgaaccttta aaaacagctt tggctacttc aatatcaaag ccagtcagtt tcccatcctt
    43321 cacataagaa aaaggtgccg ttgttccaac agttgcaaaa ttaacttctt ttttgcccgt
    43381 cttagtagta tgagattttg agctacaagc agctagagac gctactgtca ctaaaataat
    43441 tcctgttaca cttaacactt tttttattgt catctttttt ctcctcttta attgtacacg
    43501 acatcataac atctttaagt ttttaactaa tacttatctt ctatcgtctg agagtttttg
    43561 ctattatatg gatgctacgt ttcattaaca aaataaccaa ataaggtttg gttttcaatt
    43621 tggtgaagca ttaattctgg atgccattga acacctataa tacattgttc tgttcgactt
    43681 tcataagctt ctactgtccc atctctagga tctctagcac taactatcag accttcagct
    43741 aacagtttaa cactttgtct atgaagagaa ttgatcttaa aatcacctga tataaagcct
    43801 tctagaaaac tccctttttc cacatttaaa taatgagcaa atccaatgta aggctcttga
    43861 tagtgattat caatagattg attaagtgta ccaccaagcg ccacatttac taactgcatg
    43921 ccacggcaaa tcgcaaagat tggtttattt tgtttcaacg cttcttcgac taatgcaaat
    43981 tcaaaaatat cacgcgcaag tgaataatca tcactctcaa ttatcttctc ttcaccataa
    44041 taggatggta aaacattttg gccaccagat atgatcagct tatctatcat ctcaacataa
    44101 gctttggcac tttctgcttc acttattggt aggataactg gtaacccgcc tgcgttttta
    44161 actccttccg aaatatgcct agataccgaa tcataataat aacctggaat atctgacatt
    44221 tctctttcat ttcctgtaat accaataata ggctttgtta acattttgct ccctcatcaa
    44281 ccaataactg tctaataatt atcattttac acctaaaaaa actttttgaa taatattttt
    44341 tacctatcac ttctttaaca atacctatca actctcacaa aaataaaaac catagtatta
    44401 atactacggt tttatttttt agaaatctgc tacattatga taaacttttt gaacatcatc
    44461 atctgattcg agtgcgtcaa taagcttttc aaaagtttca aggtcatcac cttccaatac
    44521 tacttctgat tgaggaatca tttcaagttc agtaacttgg aattcttcta caccattatc
    44581 gcgaagtgct tggatacctt tatgaagatc tgttggggcg gtataaactg ttattgttcc
    44641 ctcttctgct tcaacatcat ctacgtctac atccgcttca agtaattgtt cgaagacagt
    44701 gtcagcatca tcaccagcaa aaacgatgac acctttttta tcaaataagt aggataccga
    44761 tcctgaagct cccatattgc caccgttctt accgtaagca gtacgtacat ttgctgccgt
    44821 acggttaaca tttgatgtca aagtatccac aataatcatt gaaccatttg gaccaaaacc
    44881 ttcatagcgt ccctctacga aagtttcatc tgtgtttcct ttggctttat caatcgcttt
    44941 atcaataaca tgctttggaa cttgtgcttg cttagcacgg tccaaaacga attttagagc
    45001 tgagtttgac tcggggtctg gttcaccttg ctttgcagca acatatattt caacaccgaa
    45061 tttagcgtat acttttgagt tagcaccatc tttagcagtc tttttggcaa caatattggc
    45121 ccacttacgt cccattggaa aatctccttt gatttcattt ttaataacct gctggctcat
    45181 attggcaagc aatcttaatt ttaactctat tattatagca ctttgatagc aattggtaaa
    45241 atggaaattc tatcacggta tgcagtagaa ttttttagaa ttgtcaattt tctattaagg
    45301 agaacctacc gagttccttt tataaaaaga ctgaacaaat tttcctttgc ccagccttga
    45361 cagattcggt gttatcctgt atcttacgat aaagactaag taagggaacg ttgtaattgg
    45421 acttactcgg taaagcgtag tggttaccat aactgcgctt cttttattcg attaattcaa
    45481 tagaattgta gcacactaca aaacgaataa cgagcttttt aaagaaaaat ataagaaaag
    45541 cccttttaaa ggtgcttttc ttatatttgt ttcaaatcaa tcaatttctt tatttcttta
    45601 atttcgacat ccttaaaagg aaccgttttt tcaaactcgc ctctattaac ttgtttaaat
    45661 cctagatctc tgtcattacc aatttgtaca tcaatagtgc ataactcaac tattttctct
    45721 ctattttttc cttctctaat aaaaaattgt gtaaatatat ggtcacaata gaagcaacat
    45781 ctttccactt gatacacctc actaatttca gtttgatcaa ccggtcgtat gtgataatat
    45841 tgatatttgc tgaaaccctt ttgccacttt ctactccata ggaaaaattt atcctgctta
    45901 ctatataacg acacctcctt tccttggaaa actgtctctc ccacatgagt cgcaccatca
    45961 agtggtgcat ctttctcatc aaacgaaatc tttttcttag acaaaatcga cattatcaac
    46021 tgtgcagaac ctattgcata aaaaattgtt cctacaataa atactagtaa aattattgtt
    46081 agtacaatga ttccaaaaac tttctgtgca tccattgagg aatacccaga caaattatat
    46141 accaatgtat atccacctac agcagttatt gataggcaaa taaaaaataa tatcaacaca
    46201 ccgtacgaag aatccatgat cttacatact aacttttctg atagaaattt ccatacaagg
    46261 cgcacaacac ttccctccct aaatatatta atataatagc tatacacatt aaagtgacca
    46321 tattcggata ttgatcatct agtctcatgt catcaaaaat caaaaactca ataaaattct
    46381 atgctacatg gctataattg aagtaagctg agttatttta gattcgtata acattacagt
    46441 agatatatat a
//
LOCUS       DATRME010000008        41346 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRME010000008 DATRME010000000
VERSION     DATRME010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41346
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(23..1804)
                     /locus_tag="VBC23_000462"
     CDS             complement(23..1804)
                     /locus_tag="VBC23_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6842435.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGHFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1974..2576
                     /locus_tag="VBC23_000463"
     CDS             1974..2576
                     /locus_tag="VBC23_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6842436.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2623..4029
                     /gene="pepV"
                     /locus_tag="VBC23_000464"
     CDS             2623..4029
                     /gene="pepV"
                     /locus_tag="VBC23_000464"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6842437.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4126..4710
                     /locus_tag="VBC23_000465"
     CDS             4126..4710
                     /locus_tag="VBC23_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6842438.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4725..5459
                     /locus_tag="VBC23_000466"
     CDS             4725..5459
                     /locus_tag="VBC23_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842439.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5628..9386
                     /locus_tag="VBC23_000467"
     CDS             5628..9386
                     /locus_tag="VBC23_000467"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO6842440.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9521..10438
                     /locus_tag="VBC23_000468"
     CDS             9521..10438
                     /locus_tag="VBC23_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO6842441.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(10480..10656)
                     /locus_tag="VBC23_000469"
     CDS             complement(10480..10656)
                     /locus_tag="VBC23_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6842442.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10767..11657
                     /gene="miaA"
                     /locus_tag="VBC23_000470"
     CDS             10767..11657
                     /gene="miaA"
                     /locus_tag="VBC23_000470"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6842443.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11748..12986
                     /gene="hflX"
                     /locus_tag="VBC23_000471"
     CDS             11748..12986
                     /gene="hflX"
                     /locus_tag="VBC23_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6842444.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12979..13626
                     /locus_tag="VBC23_000472"
     CDS             12979..13626
                     /locus_tag="VBC23_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6842445.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            13668..14597
                     /locus_tag="VBC23_000473"
     CDS             13668..14597
                     /locus_tag="VBC23_000473"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6842446.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            14599..15360
                     /locus_tag="VBC23_000474"
     CDS             14599..15360
                     /locus_tag="VBC23_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6842447.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            15357..17555
                     /gene="recJ"
                     /locus_tag="VBC23_000475"
     CDS             15357..17555
                     /gene="recJ"
                     /locus_tag="VBC23_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6842448.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIYDFMMS"
     gene            17707..20271
                     /locus_tag="VBC23_000476"
     CDS             17707..20271
                     /locus_tag="VBC23_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6842449.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            20394..20912
                     /locus_tag="VBC23_000477"
     CDS             20394..20912
                     /locus_tag="VBC23_000477"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6842450.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            21030..21710
                     /locus_tag="VBC23_000478"
     CDS             21030..21710
                     /locus_tag="VBC23_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6842451.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            21814..22497
                     /locus_tag="VBC23_000479"
     CDS             21814..22497
                     /locus_tag="VBC23_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6842452.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            22487..23275
                     /locus_tag="VBC23_000480"
     CDS             22487..23275
                     /locus_tag="VBC23_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6842453.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            23284..24387
                     /locus_tag="VBC23_000481"
     CDS             23284..24387
                     /locus_tag="VBC23_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6842454.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            24446..25315
                     /gene="rfbA"
                     /locus_tag="VBC23_000482"
     CDS             24446..25315
                     /gene="rfbA"
                     /locus_tag="VBC23_000482"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6842455.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            25315..25908
                     /locus_tag="VBC23_000483"
     CDS             25315..25908
                     /locus_tag="VBC23_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6842456.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            26115..27161
                     /gene="rfbB"
                     /locus_tag="VBC23_000484"
     CDS             26115..27161
                     /gene="rfbB"
                     /locus_tag="VBC23_000484"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6842457.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(27213..30431)
                     /gene="hylB"
                     /locus_tag="VBC23_000485"
     CDS             complement(27213..30431)
                     /gene="hylB"
                     /locus_tag="VBC23_000485"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6842458.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKTKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            30648..31130
                     /locus_tag="VBC23_000486"
     CDS             30648..31130
                     /locus_tag="VBC23_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6842459.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            31120..31581
                     /locus_tag="VBC23_000487"
     CDS             31120..31581
                     /locus_tag="VBC23_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6842460.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
     gene            31673..32863
                     /locus_tag="VBC23_000488"
     CDS             31673..32863
                     /locus_tag="VBC23_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6842461.1"
                     /translation="MNQPSEKEFKNSLFFKWILNNQAVIALMITFLVFLTIFIFTKIS
                     FMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIW
                     AMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQ
                     AVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTK
                     LRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVLNM
                     VPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPIT
                     IMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSEEV
                     KNVE"
     gene            32853..34079
                     /locus_tag="VBC23_000489"
     CDS             32853..34079
                     /locus_tag="VBC23_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842462.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            34189..35871
                     /gene="alsS"
                     /locus_tag="VBC23_000490"
     CDS             34189..35871
                     /gene="alsS"
                     /locus_tag="VBC23_000490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6842463.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            35885..36604
                     /gene="budA"
                     /locus_tag="VBC23_000491"
     CDS             35885..36604
                     /gene="budA"
                     /locus_tag="VBC23_000491"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6842464.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(36658..38313)
                     /locus_tag="VBC23_000492"
     CDS             complement(36658..38313)
                     /locus_tag="VBC23_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6842465.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            38672..39676
                     /locus_tag="VBC23_000493"
     CDS             38672..39676
                     /locus_tag="VBC23_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6842466.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            39689..40552
                     /locus_tag="VBC23_000494"
     CDS             39689..40552
                     /locus_tag="VBC23_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842467.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            40552..41313
                     /locus_tag="VBC23_000495"
     CDS             40552..41313
                     /locus_tag="VBC23_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6842468.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
BASE COUNT        13581 a         6385 c         8237 g        13143 t
ORIGIN      
        1 tctactaagc tttttattta atttaaccct cgctatcaat aagttggaga gctctttgat
       61 aataattagg gctatttttg atatctttat taatgacctt gaaaagattg agtttatctg
      121 tgatgattcc attaactggg ctttgtaaat aacgtatcat aaggttatca cggttaatag
      181 tccaaacata gagctgttta ttatattctt gggcgagata tgcaatccaa tcattgtatg
      241 aaaattcctc aatcccataa aagtcaacgt tatgattttg aagcgttccc caatgtagtg
      301 ggattaggta acctgtatca agttgtggca attttttctc aaccttttca atgagtgtca
      361 agttgagaga cattacttta tgcttggttg atagacgaag ctctttaaat tctttgataa
      421 attcatccgc atagatatcg aggttttgat tatacggttt caattcaatc atgatttttt
      481 gatctagtga ctgagcggtc ttcataaatt ctgccaatga tggaatgtaa ctagcaaaat
      541 gcccttggtg aattgggatt tttgtaactt gatctaaagt tagtttagaa acatctttgt
      601 tcataccaga gagtcgtttg agattatcat catgaatgac tactaattta tgatcttttg
      661 tcatgataac atccatctct gaataatctg cttttgcttt atgggctgct tttaatgctt
      721 caatagaatt ttcaacgcca tgtgatgtat cactacgatg ggctattaat tcttttttaa
      781 ttgaaatgtt atcttgtaag agagccagag ctatagcatt ttttaaggca gtggtaccaa
      841 tagctagagc tagaataaca caagcaatgt gtttaaattt ttgcttatgg tggacgcctt
      901 tccaagctct ccattgttga acaatacttt tattttgttt aatatctgct aataggtgac
      961 ttatgattaa acatttaata agaagtgtta cggcaaaaag tattgatttt aaaaggttat
     1021 agaaagtggc ttgaagagga aaattgtttc cagaatgatc aatgaatgaa aatgtaccag
     1081 caacaacagc tagtgcaact gttccgatta cagatataaa taaactggtt aaaataattt
     1141 tacttagaag acgccactga cgctttccgc ggctaagttt ccaactctct aaaagtgctt
     1201 ctttagcatt gtagtcagtt aaaatggtca gcgggagaaa ataaattaat cgaaggttta
     1261 agtaacaaat aagcagtaaa agggtagagt aacctatctt tccaaagact gtctttgtta
     1321 actcgccaga aataaagtca ggaattttaa gatgctctaa tatacttgac cttagaccaa
     1381 ggttagctag aggaattgtc aaaagtaagt agattgatac ccaaattagc tgtggtccta
     1441 aaagtgattt tattttttta ggaaagattg ttagccattt tttgatactg agagaaaatt
     1501 cagttttcag tatggcaata gtatagatag cgaactcagt atagatgaac ccaattagga
     1561 ttacgatata aagtataaag agtaataaag ctaagggatg attaaaaagc aaagtccaat
     1621 tgtctttagt cactgcggga gtatttgata atatcaatat ccgacgaaag ataaaactca
     1681 gaattccttt tactaaataa gcaactaaag tttggattaa gataccagtt ccaatgtaaa
     1741 cccaaaattg attttttaaa gaggttaagg ttagaaaaaa atcttctttt aatgctcgta
     1801 gcatgaatag ctcctttttt atagtcatta gatattgtac cacaagtttg tttgtttttt
     1861 atggtagttt tcaaaccggt ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc
     1921 attttaaaaa gtgatagaat agtagggaaa atcaaataga ataggaggtt tttatgaaat
     1981 ttctagaatt aaataagaag cgccatgctg tgaaacattt taatgataag ccagtcgact
     2041 ttaaagatgt tagaactgct attgaaattg cgacattagc gccatctgct aataatattc
     2101 agccttggaa gtttgttgtt gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg
     2161 aaagtaattg taaccaaatt aatcaagcgc agtatgttat tgctttattt acagatacag
     2221 atttaggaca gagatcacgg aaaatagctc gtattggtcg tagatctctt cctgatgatt
     2281 taattggtta ttatatggaa actctaccac cacgctatgc tttatatagt gaaaaacaaa
     2341 ctggagagta tctctcttta aatgcaggta tcgttgctat gaatttagta cttgccttga
     2401 cagatcaagg tattagttct aatatgattt tagggttcga taaagcgatt accaatgatg
     2461 tcttagagat agataaacgt tttagaccag aaatattaat tactgttggt tatagtgatg
     2521 aaaaggttga gcctagttat cgtttgccag ttgatcatat tattgaaaaa cgttaatttt
     2581 taagcacaaa ctacataata ttttatagaa ggagttgccg taatgacaat tgattttaga
     2641 gcagaagtag acaaacgcaa agacgctttg atggacgatt taataaatct tcttcgtatt
     2701 aactcggagc gtgatgattc acaagctgat gcagagcatc catttggtcc tggacctgta
     2761 aaagccttgg aatttttcct tgagatggct gagcgagatg gctacgaaac aaaaaacgta
     2821 gataattatg cgggacactt tacatttggt caaggcgaag aagaactagg tatttttggg
     2881 catttagatg ttgttcctgc aggcagcggt tgggatacag acccttatga accagttatc
     2941 aaggataacc gtttatatgc tcgagggtca tcagacgata agggaccaac aatggcatgt
     3001 tattatgctc taaaaattat taaagagctt gggctaccaa catctaagaa agttcgtttt
     3061 gttgtaggaa ctgatgaaga atctggatgg ggagatatgg attactactt cgaacatgtt
     3121 ggtcttccaa aacctgattt tggtttctct ccagatgcag aatttccaat tataaatggt
     3181 gaaaaaggta atatcactga atatttacat ttttcaggag aaaataaggg agctgttaga
     3241 ctgcatagct tttctggcgg acttcgcgaa aacatggttc cagaatcagc cacagcacgt
     3301 ttcacaagtc atttagatca gacaacactt ggagctagtc tagctgattt tgcaggtaaa
     3361 cataatttga aagcagaatt atctgtagaa gatgggcagt atatagctac agtttatggt
     3421 aaatctgctc atggttcaat accacaagaa ggtgttaatg gggcgactta tttagctctt
     3481 tatctaagtc aatttgattt tgaaggtcct gctcgtgctt tcttagatgt cacagccaac
     3541 attattcacg aagacttctc aggtgaaaaa cttggagtag cttatgaaga tgactgtatg
     3601 ggatcgttga gcatgaatgc aggtgtcttc cagtttgatg aaactaatga tgataatact
     3661 atcgctctta atttccgtta cccacaaggg acagatgcta aaactatcca aactaagctt
     3721 gagaaactta acggagttga aaaagtgact ctttctgacc atgaacacac accacactat
     3781 gtacctatgg acgatgaatt agtatcaacc ttactagctg tctatgaaaa gcaaactggt
     3841 cttaaaggac atgaacaggt tattggtggg gggacatttg gtcgcttact tgaacggggt
     3901 gttgcatacg gtgccatgtt cccaggagat gaaaacacta tgcatcaagc taatgagtac
     3961 atgcctttag aaaatatttt ccgttcggct gctatctacg cagaagctat ctatgaatta
     4021 atcaaataaa ataatcctta aactaaatat gtgatcaatg ataaagggtg gtgaagacat
     4081 gaaagtgtct ttgcctcttt tcataaggtt agatttggag actttatgac tgacttggaa
     4141 aaaattatta aagcaataaa aagtgattca cagaatcaaa attatacaga aaatggtatt
     4201 gatcctttgt ttgctgctcc taaaacagct aggatcaata ttgttggaca agcacctggt
     4261 ttaaaaactc aagaagcaat actctattgg aaagataaat ctggagatcg tctacgccag
     4321 tggcttggag ttgatgaaga gacattttac cattctggaa aatttgctgt tttgccttta
     4381 gatttttatt acccaggcaa aggaaaatca ggagatttac cccctagaaa aggttttgcg
     4441 gagaaatggc accctcttat tttaaaagaa atgcctaatg ttcaattgac cttgctagtt
     4501 ggtcaatatg ctcagaaata ttatcttgga agctccgcac ataaaaatct aacagaaaca
     4561 gttaaatctt acaaagacta tctacccgat tatttacccc tggttcaccc atcaccgcga
     4621 aatcaaattt ggctaaagaa gaatccatgg tttgaaaaag atctaatcgt tgatttacaa
     4681 aagatagtag cagatatttt aaaagattaa ggataggagt tagtatgaga gataatcatc
     4741 tacacacgca tttttcctat gattgtcaaa cggcatttga ggactatatt aatggtttta
     4801 caggtgaatt tatcacaaca gaacattttg atttatcaaa tccttacacc ggtcaagacg
     4861 atgttcctga ttatagtgct tattgtcaaa aaatagatta tcttaatcag aaatatggaa
     4921 atcgatttaa aaaaggaatt gaaatcggtt attttaaaga tagggaatca gatattttag
     4981 attatttaaa aaataaagaa tttgatttaa aactattgtc aatccatcat aatggtaggt
     5041 atgattatct gcaagaagaa gctctgaaag taccaacaaa ggagctttta gcagattact
     5101 ttaatcgtat ggaatttgcc ataggccgtg tggaagcgca cgttttagat cactttgatt
     5161 atggttttcg taagttaaac ttagatgtag aagatttaaa accgtttgaa acgcaattga
     5221 agcgcatttt cagaaagatg ttatctaagg ggttagcttt tgaactaaat accaaatccc
     5281 tttatctata tgggaatgaa aaactttatc gctatgcttt agagatacta aaacagcttg
     5341 gttgtaaaca atactctata ggctctgacg gtcatattcc tgaacatttt tgttatgaat
     5401 ttgatagact tcaatgtctg ctaaaggact atcaaattga tgaaaatcat ttgatatgag
     5461 gaaatttttg ataaaaaagc taggcaatat tgcttagctt ttttgtaatg tcattgatag
     5521 ttttagtgaa aatttcaaaa aaataaagaa atcacttact tgttgcaagc gcttgcgtaa
     5581 attgttatga ttttattggt aacaattcat taaaaaagga gaatgatatg aaaagaaaag
     5641 acttatttgg tgataaacaa actcaataca cgattagaaa gttaagtgtt ggagtagctt
     5701 cagttgcaac aggggtatgt atttttcttc atagtccaca ggtatttgct gaagaagtaa
     5761 gtgtttctcc tgcaactaca gcgattgcaa agtcgaatat taatcaggtt gacaaccgac
     5821 aatctactaa tttaaaagat gacataaact caaactctga gacggttgtg acaccctcag
     5881 atatgccgga taccaagcaa ttagtatcag atgaaactga cactcaaaaa ggagtgacag
     5941 agccggataa ggcgacaagc ctgcttgaag aaaataaagg tcctgtttca gataaaaata
     6001 ccttagattt aaaagtggca ccatctacat tgcaaaatac tcccgacaaa acttctcaag
     6061 ctataggtgc tccaagtccg accttgaaag ttgctaatca agctccacag attgaaaatg
     6121 gttactttag gttacatctt aaagaattgc ctcaaggtca tcctgtagaa agcactgggc
     6181 tttggatatg gggagatgtt gatcaaccgt ctagtaattg gccaaatggt gctatcccta
     6241 tgactaatgc taagaaagat gattacggtt attatgttga ttttaaatta tctgaaaaac
     6301 aacgaaaaca aatatctttt ttaattaata acaaagcagg aacaaattta agcggcgatc
     6361 atcatattcc attattacga cctgagatga accaagtttg gattgatgaa aagtacggta
     6421 tacatactta tcagcccctc aaagaagggt atgtccgtat taactatttg agttcatctg
     6481 gtaactatga ccacttatca gcatggctct ttaaagatgt tgcaaccccc tcaacaactt
     6541 ggccagatgg tagtaatttt gtgaatcaag gactatatgg aaggtatatt gatgtaccac
     6601 tgaaaactaa tgccaaagag attggttttc taatcttaga tgaaagtaag acaggagatg
     6661 cagtgaaagt tcaacccaac gactatgttt ttagagattt agctaaccat aaccaaattt
     6721 ttgtaaaaga taaggatcca aaggtttata ataatcctta ttacattgat caagtgcagc
     6781 taaaggatgc tcaacaaact gatttaacaa gtattcaagc aagttttaca actctagatg
     6841 gggtagataa aactgaaatt ttaaaagaat tgaaagtgac agataaaaat caaaatgcta
     6901 tacaaatttc tgatatcact ctcgatacta gtaaatctct tttaataatc aaaggcgact
     6961 ttaatcctaa acaaggtcat ttcaatatat cttataatgg taacaatgtc acgacaaggc
     7021 aatcttggga atttaaagac caactttatg cttatagtgg aaatttaggt gcagttctca
     7081 atcaagatgg ttcaaaagtt gaagccagcc tctggtcacc gagtgctgat agtgtcacta
     7141 tgattattta tgacaaagat aatcaaaaca gggttgtagc gactaccccc cttgtgaaaa
     7201 ataataaagg tgtttggcag acgatacttg atactaaatt aggtattaaa aactatactg
     7261 gttactatta tctttacgaa ataaaaagag gtaaggataa ggttaagatt ttagatcctt
     7321 atgcaaagtc attagcagag tgggatagta atactgttaa tgacgatata aaaacggcta
     7381 aagcagcttt tgtaaatcca agtcaacttg gacctcaaaa tttaagtttt gctaaaattg
     7441 ctaattttaa aggaagacaa gatgctgtta tatacgaagc acatgtaaga gacttcactt
     7501 ctgatcaatc tttggacgga aaattaaaaa atcaacttgg tacctttgca gccttttcag
     7561 agaaactaga ttatttacag aaattaggag ttacacacat tcagctttta ccggtattga
     7621 gttattttta tgttaatgaa atggataagt cacgctcaac agcttacact tcctcagaca
     7681 ataattacaa ttggggctat gacccacaga gctattttgc tctttctgga atgtattcag
     7741 agaaaccaaa agatccatca gcacgtatcg ccgaattaaa acaattaata catgatattc
     7801 ataaacgtgg catgggggtt atacttgatg tcgtctataa tcacactgca aaaacttatc
     7861 tctttgagga tatagaacct aattattatc actttatgaa tgaagatggt tcaccaagag
     7921 aaagttttgg agggggacgt ttaggaacca ctcatgcaat gagtcgtcgt gttttggttg
     7981 attccattaa atatcttaca agtgaattta aagttgatgg tttccgtttt gatatgatgg
     8041 gagatcatga tgcggctgcg attgaattag cttataaaga agctaaagct attaatccta
     8101 atatgattat gattggtgag ggctggagaa cattccaagg cgatcaaggt aagccggtta
     8161 aaccagctga ccaagattgg atgaagtcaa ccgatacagt tggcgtcttt tcagatgata
     8221 ttcgtaatag cttgaaatct ggttttccaa atgaaggtac tccagctttc atcacaggtg
     8281 gcccacaatc tttacaaggt atttttaaaa atatcaaagc acaacctggg aattttgaag
     8341 cagattcgcc aggagatgtg gtgcagtata ttgctgcaca tgataacctt accttgcatg
     8401 atgtgattgc aaaatcaatt aataaagacc ctaaggtagc tgaagaagat attcatagac
     8461 gtctgcgttt aggaaatgta atgattttaa catctcaagg gacagcattc attcattctg
     8521 gtcaagagta tggtcgtacg aagcgtttac ttaaccctga ttacatgaca aaagtttcag
     8581 atgacaaatt gcctaataaa gcaacactta ttgaagctgt taaagaatac ccatatttta
     8641 ttcatgattc atatgattct tcagatgcca ttaatcattt tgattgggca gcagccacag
     8701 ataataacaa acacccaatt tcaacgaaaa cacaggccta tacagcaggt ttaatcacat
     8761 taaggcgttc aacagatgct ttccggaaat tgagcaaagc agaaattgat cgtgaggtta
     8821 gcttgattac agaggtaggt caaggtgata ttaaagaaaa agatttggtt attgcttacc
     8881 aaacaataga ttctaaaggc gatatttacg cagtatttgt taatgctgat agtaaagcta
     8941 gaaacgtttt actaggtgaa aaatataaac accttttaaa agggcaagta attgttgatg
     9001 ctgatcaagc ggggattaaa ccaatctcaa ctcctagagg tgttcatttt gaaaaagata
     9061 gtttgctgat tgatccatta acagcaattg tgattaaagt tggcaaagtt gctcctagcc
     9121 ctaaggagga attgcaagca gattatccca aaacacaatc tttcaaggga tctaaaacgg
     9181 tagaaaaagt aaatagaata gctaataaga cctcaataac tcctgtagtt tctaataaga
     9241 ccgattcata tctgacaaat gaagctaatt tgccaaaaac tggagataag tcatcaaaaa
     9301 tactaagtgt agtaggaata agcattctag caagtctact tgctctacta ggtctctctt
     9361 taaagaggaa tcgtcactta aaataaaagc aaactttaga agagcttaaa aaagaagtta
     9421 gtcaatgggc tagcttcttt tcattaagtt aataaaatca cttatcagca aaatctaatt
     9481 gtgttataat ggatgtaaac gtttttttat gaggtatatg atgaaaaatt gggaaaaacc
     9541 accattagtg atggcgggat tagtattggg gctattagct ttggggaacc ttctagaggg
     9601 atatggcacc tattttcgct attgtttagg gctagtagca cttgtttttt ggattttttt
     9661 aatcaaagga atattaaaaa ataaaaaaga aagcaggaag gagttaagca atcctttgat
     9721 tgcttctgtc tttactactt tttttatggc aggtatgatt ttatcgacct atatcttatt
     9781 atttagaagt ttaggaatat gggtagcagt tctgtcaaaa ggtgtatggt ggttgtcatt
     9841 tattgcgtta atcattcata tggctatttt ttcatggaaa tatttgagac acttttcgat
     9901 ggctaatctt tttccttctt ggtctgtcct ctatgttggt ataggggtag ctagcctgac
     9961 agcaccaatt agtggacaat tcactattgg gaaaatagtt ttttggtatg gttttattgc
    10021 aactttagta ttattaccat ttctattcat taaagcatat aaaatcgggc taccttcggc
    10081 agttaagcct aatataacta ctatttgcgc tccaatgtcg ctaatcacag cgggatatgt
    10141 taatagtttt gtgtcaccaa atagaggttt attattgcta ttaatcgtaa tggcacaatt
    10201 tctttatttt ttcattcttt ttcaggtgcc taaattactg attggtgatt ttacaccagg
    10261 tttctcagcc tttactttcc cccttgttat ctcagctacc tcacttaaat taagtattca
    10321 gcatctatca ttaccagttg atatacaggg gctagtccac tttgaaattg ggacaacaac
    10381 tcttattgtt atgatagtaa tggtacgtta tattttcttt ttaaggcgaa caatttaata
    10441 taaaataact cccttactag ctagtaaggg agttattgtt taatgagaga cacgtttacg
    10501 tgcagctttt ttacgatttt cttcgataaa agctattttt tcttcctcag gttcaataat
    10561 tgtttttttg atagcaaatg cagcacctgc aagagtagca gctgttgcaa cgactcctgt
    10621 tgctattcct ttagcaaatg cgaatttttt aaccataata attctccaat ctatgatgaa
    10681 gactctgctt ctatctatga tataattatg gtaacgaaat actagttaaa tttcaagtaa
    10741 aaacctttat agaattgaga agtatcatga gaaaaatcaa gcttatagca gttgttggtc
    10801 caactgcggt tgggaagaca gcattaggaa ttgaattagc taaaactttc aatggcgaaa
    10861 tcattagtgg cgatagtcag caagtttatc agaagttaga tatcggaacg gctaaagcta
    10921 gtaaagaaga gcaagaacaa gcttatcatc acttgattga tgttcgtgaa gtaaatgaga
    10981 attattcggt ttatgatttt gtcaaagagg caaaagtagc tattgatacg attatttcta
    11041 aggggaaaat ccctattatt gttggaggta ctggcctata tttacagagt ttgtttgagg
    11101 gctatcattt aggaggcgaa gtcaatcaag aaactcttaa ggcctatcgt gaaaaattag
    11161 agtctctaag tgatgaagat ttatttgaaa agttgacaga acaatccatt gttataccgc
    11221 aagttaatcg tcgacgtgcc atccgtgcat tagaattggc gaagtttggg aatgatttgc
    11281 aaaatagtga aagtccgtat gatgttttat taattggatt gaatgatgat cgtcaggtgc
    11341 tctatgatcg tatcaataga cgagttgatt tgatgataga caatggtctc ttagatgaag
    11401 ctaaatggct atacgataac taccctagtg ttcaagcaag tagaggaatt ggctataaag
    11461 aattatttcc ttacttttca aagcaaatac cattagaaga agctgttgat aaactaaaac
    11521 aaaataccag acgttttgca aaacgccaat taacatggtt tagaaaccgt atgaatgtcg
    11581 aatttattat ggttggtgag gaaaattatc aacaaaaaat caagcgaaaa gtcagtgatt
    11641 ttttatcatc aaagtaaata aagccgtttc tcgcatttaa agggcattat gttataataa
    11701 agattacgta tttagaacaa gaaaaaaata ttgaaaggag attataggtg atagaaacta
    11761 aagaagagca agaacgtgtt attttagttg gggtagaact tcaagatact gagaattttg
    11821 aaatgtccat ggaagaactt gctagcttag ctaagacagc aggagcaaat gttgttaatc
    11881 attattatca aaaaagagat aaatatgata gtaaatcatt tatcggatct ggtaaattag
    11941 aggagataaa agctattgta gaagcagatg agattgatac cgttgtggtt aataatcgtt
    12001 tgactccgcg acaaaactct aatttggaag ctgaacttgg tgttaaagtt attgaccgta
    12061 tgcaacttat tttagatatc tttgctatgc gtgctagaag tcatgaagga aaattacagg
    12121 ttcatctagc gcaattgaaa tatatgttac cacgcttagt tggacaaggt attatgctta
    12181 gtcgtcaagc aggaggaatc ggaagccgag gtccaggaga atctcagtta gagttgaatc
    12241 gtcgttccat tcgtcatcaa atttctgata tcgaaagaca attgaagatt gtagaaaaaa
    12301 atcgcgagac agttcgagaa agacgtgttg attcgacaac ctttaaaatt ggattaattg
    12361 gatatacaaa tgctggcaag tcaactatta tgaatgtttt gaccgatgat aagcaatatg
    12421 aagccaatga attatttgca acgttagacg ctacaaccaa gcagatctat ttacaaaatc
    12481 aatttcaagt cactttaaca gatactgttg gttttattca agatttacct acagagttag
    12541 ttgcggcttt caagtctacc ttagaagaaa gtcgacatgt tgatttatta cttcatgtta
    12601 ttgatgctag tgaccctaat catgaagaac acgaaaaagt tgttatggag attcttaaag
    12661 acttagatat gatagatatt cctcgtttag cgatatacaa caagatggat gtcactgagc
    12721 agctgaatgc aacaactttt cctaatgtga gaattgcagc caaaaaacaa gggagtaggg
    12781 atttattacg ccgtcttatt gtggatgaaa tacgacatat ctttgacgaa ttttcgatta
    12841 gagtgcatca aaatcaagca tataagctct atgatctcaa taaaatagca ttgcttgaca
    12901 cctacacctt tgaagaagaa tacgagaata ttacaggcta tatttctcct aaacaaaaat
    12961 ggaagctgga agaattttat gattgattat attgatttgg ctttgactta tggtggtttt
    13021 acgagcttag ataaagttta tctagagaaa aaactagatg gcctctctaa gcaacaacga
    13081 cttgatttta tcactccacc tcctagtgtg attaatgctt attttgctga aatatatcaa
    13141 aagcaagggc cagaagcggc aactgattac tattttgatc tttccaaagc cttgggcttg
    13201 tttccaaaac atttatcgtt tgatgaagaa aaacctttta ttcgtttgaa tttatcagga
    13261 aaatcatttg gttttgctta cctaaatgat caagaagaag cgagtgtttt ttctgaagtt
    13321 aaagaagtca ttacaccgca gttactctta gaaattgcac agatttttcc tcaatataaa
    13381 gtctatcaag atcggtctgg tatcagaatg gcaaaaatag attttgacga aacagaatct
    13441 caaaatatta caccagaaac aagtttacta gggaatgtct tacaacttaa aaaagatatt
    13501 attaaaataa ctagtttcaa tcaagaagag ctattagagt tggttaaaac aaagtcaggt
    13561 gaatactatt attctagtca aggacgtgag tcagttattt atattgatac taaatctact
    13621 aattaaattt attaagttat ggaaggatta taaaaaagtc aacaagtatg gaaattcaat
    13681 ttttaggaac tggagctggt caaccagcga aagctcgtaa tgtttcaagt ttagtcttga
    13741 aattattaga tgaaattaac gaggtttgga tgtttgattg tggagagggt acacagcgtc
    13801 aaattttaga aaccacgatt aaacctcgta aagtcaaaaa aatttttatc acgcacatgc
    13861 acggagacca tgtttttggt cttccaggtt ttttatccag tcgggctttt caagccaatg
    13921 aagaacagac agatttagat atttatggac cggtcggtat aaaaagtttt gttatgactg
    13981 gcttaaggac atcaggttca cgtttacctt atcgaattca ttttcacgag tttgatgaat
    14041 catcactcgg aaaaattatg gaaacagata aatttaccgt ttatgcagaa aagctagatc
    14101 atactatctt ttgtatggga tatcgtgttg tgcaaaaaga ccttgagggc actttagatg
    14161 ctgaagcatt gaaactagca ggtgttccat ttggtcccct ttttgggaaa gtcaaaaatg
    14221 gtgagaatgt taccttagaa gatggtagag aaattattgc taaagattat atttctgaac
    14281 cgaaaaaggg taaagtaatc actattttgg gagatactag aaaaacagat gcaagtatta
    14341 ggttagcttt gggcgctgac gttttagtgc acgaatctac ttatggtaag ggtgatgagc
    14401 gaattgctaa atcgcatggg cattcaacca atatgcaagc agctgatatc gctaaacaag
    14461 caaatgctaa acgactatta ttaaatcatg ttagtgctcg ttttatgggg cgagattgtt
    14521 ggcaaatgga agaagatgca aagacgattt tttcaaatac ccatcttgtt agagatttgg
    14581 aagaagtagg tatataaaat ggcaagaacg atactgatta caggagcttc tggtggttta
    14641 gcacaggcaa ttattaacca attacctcaa gacgaccatt taattgtcac agggagatca
    14701 agagaaaaac ttgaaaagct ttatggaaag agaccaaaca ccttatgttt atcactagac
    14761 ataactaatg acaatgctgt aacgaatatg attgaaaaaa tttatgttga atttggtcaa
    14821 attgatattt taattaataa tgctggattt ggttctttta aagaattttg ggattatagt
    14881 gatgaagaag tcaaagacat gtttgctgtg aatacatttg caacgatgtc tatagctcgt
    14941 cagataggac ataagatgtc tcttgtaaaa tctggtcata tcgttaatat tgcaagcatg
    15001 gctggcctca tagcaacaag taaagctagc gtttatgggg ctagtaagtt tgctgtcgtt
    15061 ggatttttga atgctcttcg tttagaatta gcagaaaaaa atgtttatgt gacaagtgta
    15121 aaccctggac caatcaaaac aggatttttt gctcaggctg atccaagtgg tgattattta
    15181 gcaagtatag gtcgttttgt tctaacacca gaaaaagtag ctaaaaaagt agtttctatt
    15241 cttggaaaaa ataagagaga attaaatctt ccttttatat tagcttttgc gcataaatat
    15301 tatagtttat ttccaaaaac agctgattat tttgccagaa aggtattcaa ttataaatga
    15361 tatctgctaa gtatagttgg gtcctcaata accaaaagcc agacgctggc ttttttgagg
    15421 cttcaaaaaa agaaaagata agtgaagcgg tggcttcctt aatttattcg cgaggtatta
    15481 agacatcagc ggaattacat cattttttac aaactaattt ggaaaatctc catgatcctt
    15541 atttgttaaa tgatatggat aaagctgtta atcgtattag acgagctatt gaaaataatg
    15601 aaactatttt agtttatgga gactatgatg ctgatggaat gacatcagca tcaatcatga
    15661 aagaagcatt agatatgatg ggagctgaag tccaggttta tctcccaaat cgctttacag
    15721 atggttacgg tcccaatcaa agtgtttata aatattttat tgaacaacaa gatgtttcat
    15781 taatcattac ggttgataat ggtgtggcag ggcatgaagc aattgcatat gctcaaaatc
    15841 aaggtgtgga cgtggtagtt actgatcatc acagtatgcc agcagattta ccttgtgctt
    15901 atgctattat tcacccagaa catcctgatg ctaattatcc atttccttat ttagcagggt
    15961 gtggtgttgc tttcaaagta gcttgtgctc tcttagaaac aatcccgaca gaaatgttgg
    16021 atttggttgc tattgggacg attgcagata tggttagcct aactgatgaa aatcgtatca
    16081 tggtaaaagc tggacttgaa gttatgaaag atagtgaaag aataggctta caggagctaa
    16141 tatctttatc aaatattgat ttaaaaacac taaatgaaga aactattgga tttaaaattg
    16201 ctccacaatt aaacgcttta ggaagacttg atgaccctaa tccagctatt gaattactaa
    16261 cagggtttga tgatgaagaa tctcaagcaa tagcccaaat gattgatcaa aaaaatgaag
    16321 agcgcaaaga aatcgttcaa actatctttg accaagctat gcaaatgctt gatcaaacaa
    16381 agcctgtaca agttttagca aaagaaaatt ggcatccagg ggttttaggt attgtggcgg
    16441 gtcgtatttt agagagaact ggacaacctg ttattgtttt gaatattgaa gatggtattg
    16501 ctaaaggaag tgccagaagt gtagaagcac ttgatatttt tcaagctttt gaccagcatc
    16561 gagaattatt cattgctttt ggaggtcaca gcggagcagc aggcatgact ttagaggaaa
    16621 gtaaggtagg tgatctttct caagttttat gtgactatat ctctaaaaaa caattggaca
    16681 tgagtcagaa aaaaactctg acaattgata gtgagctacg ttttgacgaa cttagtttag
    16741 acactgttag agattttgaa aagttagcgc catttggaat ggataataaa aaaccagtct
    16801 ttttgttaaa agattttaaa gtgagtcaag ccagagtcat ggggcaaaac ggtgctcatc
    16861 tgaaattaaa actagaacaa gatggacaag cacttgattt ggtagcattt aatatgggga
    16921 gtcaactaca agagtttcaa caagcacaac atttggagtt agctgtcact ttatctgtta
    16981 atcaatggaa tggtgccaca accctacaat taatgttgga ggatgcgcgt gtagatggta
    17041 ttcaattatt tgatattaga agcaaagcaa gtagcttacc tcatggagtg ccaatcctgt
    17101 ctcaagaaga gcagagcaaa gaagttattc ttttgactgt tcccgatcat ccacaagaat
    17161 taaaacaaat gactcaaggt aaacagtttg atgctatcta ttttaaaaat gagataccaa
    17221 aaaattattt tatatctggt tatggtacac gtgatcaatt tgctagttta tataaaacaa
    17281 tatatcaatt cccagaattt gacgttcgtt ataagttaaa agagctcagt tcctaccttc
    17341 atattcctga tattttatta attaaaatga ttcaaatttt tgaagaacta cattttgtta
    17401 cgattacaga ggggattatg acagtcaata aagaagcaga aaagcgagat atctctgaga
    17461 gtcaaattta tcaagaatta aaagagacag ttaagtttca agaattaatg gctttaggga
    17521 cacctaaaga aatatatgat tttatgatga gttgataata tactaaagaa gccacttttt
    17581 aagtggtttt tttggtatat aaataagtta tttcaataat aatattgcaa atattattaa
    17641 aatataataa aattaagaaa ataatggaat ttttgataaa gaatattaaa ggtgaggata
    17701 aaatctatga gacctatcgt tagaaaaaag atgtacaaaa aaggtaaatt tttggtcatt
    17761 gcaggtatcg ttactatttt aggtggtagt gcaattcttg gtcaagatgt caaggcagaa
    17821 caggcagaag cagtaacttc aacgatctca gagaaaactg atagcagtca gactatttcg
    17881 gatatttcca agttaacgtt acctgttaat tcctcggagg ctatgaaaaa tagtgcagag
    17941 cctttaataa aaacaggctt tgctacatct gtttcatcta atcctagaga gatagcagct
    18001 accccagtca aaacctttga tgctagttca aaagttgttg taaaagctag tactgctgag
    18061 cactcagcaa atcaaacaaa ttctaatgtt aatcaagtag ctaatgatag tgaagttatt
    18121 actcaacaaa attcaacaaa gcaattacca acagtgactt actctgctca tgttcaagat
    18181 attggatggc aaaagtcggt tgataatgca acagtttcag gaacagtagg acaggaaaag
    18241 caggttgagg caataaaatt gtctattaaa gcccctgaag gaataacagg aaaacttagc
    18301 tataaaactt atgttaaagg acaaggttgg caaccttcag tagagagtgg tcaagttagt
    18361 ggtactgttg gtcagagtag acctattgaa gcactaagca ttaaccttac tgataattta
    18421 caaaaattat atgatgtcta ttatcgtgtt catgttcaag atatcgggtg gatggcttgg
    18481 gctaaaaatg gagcttacgc tgggactttg ggaatgtcaa agcgccttga agcttacaaa
    18541 gtaaagttta cccttaaagg acaatctgtt ttaacaccaa ctatacttaa ggaagagaga
    18601 cctgtcttaa attatcaggt taaggtaggc caaaatggtt ggcaatctaa taaacttgaa
    18661 ggacaaatgg cgggaacttt aggagaatcc aaagccctag atggagtaaa attcactctt
    18721 agcaccttga agtatgggga cattctctat cgtactcatg tacaagataa aggttgggga
    18781 gaaattttag gtagtcaaac aagtaaggat tatcaaggaa aaagactaga agctattcaa
    18841 ttggacttaa gtgggaattt aaaacaaaat tatgatattt attatcgcgc tcacgtccaa
    18901 gataaaggtt ggatggcttg gcaaaaaaat aatagtgtag caggtacagt cggagaatcg
    18961 aaacgtttag aggctttgga agtaaaacta gtagctaaag cttcagattt tagttcccaa
    19021 acaaatcatt cattcttaga aatgaagcat cctggattaa gttatcaaac ttatcttcaa
    19081 aaagatggtt ggaaaccaac ggttttggaa ggccaattag gaggtagtat tggactttca
    19141 aaatctataa aagccatcaa acttaactta ggtagtacag ctctaggaaa tatagaatat
    19201 agaacttttc ttaatggttc aggttggcaa actgttgtta attcagggag agaaagtaat
    19261 gtaccaaatg aatctcaaca agtagaagct attcagatgc gattgacagg tctgctatcg
    19321 aaaacctata gtgtgatgta tcgagttcac atgcaagatt atggttggca ggattggact
    19381 tataacaatg acatcgctgg tagtactaac caaggaaaac gaattgaagc aatcgaaatt
    19441 aaattagttg agactgctaa aatgcctata actgtccaaa cgacctataa gggaactgga
    19501 aactataatg tacgagtgac aaatgttatt agtcctggga atttaaaaat tgctatttgg
    19561 tcagataaaa acaatcagga tgaccttaag tggtataccg tcacacccaa aaatcatgaa
    19621 gctagattga catttaatgt tacaaatcat agagacaatg gtaaatactt tatccatgtt
    19681 tatcaggaaa atgcttctaa tcaaaaacaa ttattggtaa aaacttctct acaagtcgca
    19741 cattcaaatt ataatactcc ttactttagc caacgtgatg gaaggtgggc aagtcgaaga
    19801 tatggtatgg caactttagg agaaactggt tgtgtaccaa cttctctagc tatgattttg
    19861 tcttctttga aaggagaaac tgttttacca acgcaaattg cagattacct ttaccataag
    19921 acagttgaat ttaatcgtgg tgttcaagga acaacttcta gaggaatctt aaaagcagcc
    19981 aaagaatggg gtgtaacagc aactgcatta ggaactcaag ctaaccttgt acaagcgttg
    20041 aaggatggtt atcatgtcct agcggcagta caaaataatg tttttgttct tcatggaagt
    20101 catgaaatcg ttctcaaagg atataataac ggactgacac atgtctctga cccttacaca
    20161 ccgtccttgt ctggttggta tccaatttca caactttgga aagaacagag ctattatagt
    20221 gaggatagaa ttgatattgg agcacctttt gtcaaggtaa cagatgccta atttttaaaa
    20281 ccatatgcaa aactagcata tggttttaaa gtttccagta ctattttatt taattttatg
    20341 atataatgaa gagtaaaaca ttttaggggt ttccccttag aaagacaagt gaaatggatt
    20401 taaataatta catcgcttca attgaaaact atcctcaaga aggaattact ttccgtgata
    20461 tctccccatt aatggctgat ggtaaagcat acagctatgc tgttcgtgaa attgtacaat
    20521 atgctgctga taaagatatt gatatgattg ttggaccaga agctcgtggc tttattgttg
    20581 gttgcccagt tgcttatgct cttggtattg gttttgctcc agtccgtaaa ccaggtaaat
    20641 taccacgcga agttatttca gctgactatg aaaaagaata tggtcttgat acattgacaa
    20701 tgcacgcaga tgctattaaa ccaggtcaac gtgttttaat tgttgatgac cttttagcta
    20761 ctggtggaac agtaaaagcg acaattgaaa tgattgaaaa acttggtggc gttgtcgcag
    20821 gctgtgcttt cctagttgaa cttgatggat taaatggtcg taaagctatt gaaggttatg
    20881 atactaaggt attgatgaac tttccaggat aaggcctatt caagatagat gaatgataag
    20941 ggcgcctcta agtgctatga taatagttga gaggtgcttt ttgttacaca tttcataatt
    21001 ttaggacgat agagaggatt tgaaaggtta tgacttattt ggaacaatat cagtcagggc
    21061 aattgactct accaagtgct ttattttttc actttaaatc tatttttaag acagcagacg
    21121 attttttagt ttggcaattt ttctatttac aaaatacaac taatttatcc gatctaacac
    21181 cgagtcgtat tgcgacatca cttgataaaa cagtggcaga tatcaatcgt tctatatcga
    21241 atttaacaag tcaaggttta ctagatgtca aaacaattga gttgaatcac gaaatcgaga
    21301 ttatctttga tacaagccct gtgtttgcta aattagataa acttttcgaa gaggataatc
    21361 aagtaataat agacaataaa accgctgatt cgaatcgtct gaaagattta gtaggagact
    21421 tcgaacgtga attaggtcgc ttattaagtc cttttgaatt agaagattta caaaagacac
    21481 tgcaagaaga tcagacagat cctgatattg ttagggcagc tcttagagaa gcggttttca
    21541 atgggaaaac aagttggaat tatatcaatg ctatccttcg caactggcgg cgtgaaggat
    21601 tgacgacact tcgacaaatt gaagagagaa aacaagcacg tgaagataac caaatgaaag
    21661 atttagcgat ttcggatgat tttaaaaatg caatgaattt atgggaataa gtttactagg
    21721 aggagacaga atggttgata cattaggtct gctccttttc ttttgatgtt aatgatgtta
    21781 tacttttaac agagaaaaac tgaaggaaat acaatggatt tacaattatc aaaacgatta
    21841 caaaaagttg caaactatgt tccaaaagga gctcggttat tagatgtagg aagtgatcat
    21901 gcttacttac ctattttttt gttgcagatg ggatactgtg attttgccat tgctggtgaa
    21961 gtggtgaatg gaccgtatca gtctgcgttg aaaaacgttt cagaacatgg tctcacttca
    22021 aagatagatg ttcgcttagc aaatggtttg tcagcttttg aagaagcaga taatattgat
    22081 acgatagcta tctgtggaat gggtggtcgt ttgattgctg atattttgaa taacgatata
    22141 gacaaacttc agcacgttaa aacactagtc ttacaaccta ataatcggga agatgattta
    22201 agaaaatggt tagctgcaaa tgattttgaa atagtagcag aagatatctt gactgaaaat
    22261 gacaaatgct atgaaatttt agttgttaag catggtcata tgaatttaac tgctaaagaa
    22321 ttgcgatttg gtccattttt attatccaat aacacaacag tttttaaaga aaaatggcaa
    22381 aatgagctta ataaattaac ttttgcttta aactctattc ccaatagtaa aatggaagaa
    22441 cgtgctattt tggaagataa aatacaggat ataaaggagg ttcttgatga aagctagaga
    22501 acttattgat gtatatgaaa cttattgtcc acaggaatta tctatggaag gagatatttc
    22561 aggtcttcaa attggatctt tagataaaga aataaagaca gtcatggtag ctttagatgt
    22621 tcgagagact acagtagctg aagcaattga acgtcaagta gatcttctta ttgtaaagca
    22681 tgctcctatt tttcgtccat taaaggattt ggtagcaaca ccgcaaaata agatttatat
    22741 agaccttctt aaatcagaca ttgctgttta tgtgagccat actaatatag atatcgttcc
    22801 taatggactt aacgactggt tttgtgaatt attagatatt caatatcccg atatattatc
    22861 tgagacaagt aatggttatg gtattggtcg cattggtgat gttagacccc agagttttga
    22921 attttttgca tggaaaatta aagatgtgtt tggtttagat agcgttcgtt tagtttcata
    22981 tgataaatct aatcctgaaa ttcaacgagt tgctatttgt gggggtagtg ggcaaagttt
    23041 ctacaaagaa gctattgcaa aaggagcaga tgtttttgta acgggagata tttattatca
    23101 tactgcacaa gaaatgatca caaatggttt gcttgcgatt gacccaggcc atcatattga
    23161 ggttttattt gtttctaaaa tagctactat gattgagcag tggaaacttg aaaaaggctg
    23221 ggatatttca gttttagaga gtaaagctcc aacaaatccc ttttaccata tgtaggagct
    23281 attatgaaaa agatagcaat tattggtgca ggagcagtcg gagcgacact agcctattac
    23341 ttatcaaaag aaaaggatat tcaggttact gtttttgatt atggagttgg acaagcaact
    23401 aaggctgctg cggggattat tagcccatgg ttttcaaaac gtcgtaataa agcctggtat
    23461 cgcatggcga ggttgggggc tgatttttat tcaaagctag tcacggattt acaaaaagat
    23521 ggttttgaaa caaaatttta tcagcaaact ggtgttttct tattaaaaaa ggacgagagt
    23581 cagttagagt cattgtttgc actagcagat aaacgtcgac tagaatctcc tctaataggt
    23641 gatttacaaa tactaaataa aagtgaagca aatactcact ttcctgagtt agatggctat
    23701 gaacagttac tttatgcgag tggaggagcg cgagtagaag gggcggactt aacacgaata
    23761 ttgttagaag caagtggtgt taatgtcatc aaggatgaag ttcactttac gataactgat
    23821 aatggctttc gtgttcaagg aattgatttt gataaacttg ttttagcgag tggagcctgg
    23881 ctagctaaga tattagatga gcacaactat caggtagata ttcgccctca aaaagggcaa
    23941 ttgcgagact attattttag caatattaat acaggaaaat atcctgttgt aatgccagaa
    24001 ggcgagctag atattatccc atttgataat ggtaaagtct ctgtaggtgc aagtcacgaa
    24061 aatgatatgg cttttgattt aaacattgat tttaaagtct tagacaagtt tgaagagcaa
    24121 gctattggct attttccgca attaaaaaaa gcagatacga cttctgaacg tgttggaatt
    24181 cgtgcctata caagtgattt ttctccattt tttggccctg tcccttgtat ggagggtgca
    24241 tatgctgcaa gtggattagg atcaactggc ttaacagtgg gcccattaat tggttatgaa
    24301 ttatgtcaat taatcttaaa taaagaaaat cagctaaatt tagaggatta cgatattacg
    24361 aaatacgtga aaaagattag tagatgatag cgtttaaaaa aagtgttata atatgtagga
    24421 ttaatataaa taaggagacc ttactatgaa aggtattatt ttagcgggag gttcaggtac
    24481 tcgcttgtac ccattaactc gtgctgcatc aaaacaattg atgccaattt atgataaacc
    24541 aatgatttat tatccattgt cagttttgat gttggcagga ataaaagaaa tactgattat
    24601 ttctactcca caagatttgc ctcgctttga agatatgctt ggagatggtt cagaactagg
    24661 aatctctctt tcttatgctg aacaaccaag cccagatggg ttagcacagg ccttcattat
    24721 cggtgaagaa tttattggcg atgatcacgt tgccttagta ttaggtgata atatttacca
    24781 tggaccaggt ttatctgcaa tgctacaacg tgcagctagt aaagaaagtg gagcaaccgt
    24841 ctttggctat caagtaaaag atccagaacg ttttggtgtt gtcgagtttg atactgatat
    24901 gaatgccatt tctattgaag aaaaaccagc gcaacctaag tctaattatg ctgtcacagg
    24961 tctttatttc tatgataacg atgtggttga aatagctaaa aatattaagc caagtcctcg
    25021 tggagaactt gaaattactg atgttaacaa agcctacctt gatagaggtg atttatcagt
    25081 tgagttaatg ggacgtggat ttgcgtggtt agacactgga actcacgaaa gtcttcttga
    25141 agcagcacaa tatattgaaa cagtacaacg catgcaaaac gttcaagttg ctaaccttga
    25201 ggaaattgct taccgcatgg gctacatcac tcgtgaacaa gtccttgagt tggcacaacc
    25261 acttaagaaa aatgagtatg gtcaatacct acttcgtttg attggagaag cctaatgact
    25321 gaacaatttt ttgataaaga attaacttgt cgcccaattg aagccattcc tggattgttg
    25381 gaattcgata ttaccgttcg tggagacaac cgtggatggt tcaaagagaa ctttcaaaaa
    25441 gaaaaaatga taccgcttgg tttcccagaa agcttctttg aggcagacaa actacaaaat
    25501 aatatttcgt ttaacaaaaa aaatactttg cgaggtctcc atgcagagcc ttgggataaa
    25561 tatgtttcga tcgctgatga aggacgtgtg atcggtactt gggttgacct ccgtgaaggt
    25621 gacagttttg gtaacgttta ccaaacgatt atcgatgcct caaaaggtat ttttgttcca
    25681 cgcggcgttg ctaatggttt ccaagttctt tcagataaag cagcttatac ttatctcgtt
    25741 aacgattatt gggcacttga actcaaacca aaatatgctt tcgttaacta tgcagatcca
    25801 aatctaggca ttcagtggga aaaccttgaa gaagcagaag tctcagaagc agataagaat
    25861 cacccacttc tcaaagatgt aaaacctttg aagaaggaag atttgtaatt ttcaacttct
    25921 taaaaggcat taaccttagc aagtaaaaac gaattagcaa tgttgtttga cgcaagggtg
    25981 aaaggaaaca tgcaaaaggc ctgttccctc agggatttga aatattgatt tcttaggcat
    26041 gacattccat agaactactt ctaacagaac agtatccttc aaacagcaaa aagaaaaaat
    26101 ttttcggaga aaaaatgact gaatttaaaa acattatcgt aactggtgga gctggtttca
    26161 tcggatcaaa ctttgtacac tatgtttaca ataaccaccc agatgttcat gtaactgtcc
    26221 ttgataaatt gacatatgca ggtaaccgtg ctaacattga agccattctt ggtgatcgtg
    26281 ttgagttagt tgttggtgac atcgctgacg ctgaattggt agacaaattg gctgccaaag
    26341 cggatgctat tgttcactat gcggctgaga gtcacaacga taactcattg gaagatccaa
    26401 gtccatttat ccatacaaac tttatcggaa cttacacttt gcttgaagca gctcgtaaat
    26461 acgatatccg tttccaccac gtgtcaactg atgaagttta tggagatctt ccacttcgtg
    26521 aagaccttcc aggaaatggt gaaggaccag gtgaaaaatt cactgctgaa acaaaataca
    26581 atccatcatc accttactca tcaactaagg cagcttctga ccttatcgtt aaggcatggg
    26641 tacgttcctt cggcgtaaaa gcgaccattt cgaactgttc aaataactat ggaccatacc
    26701 aacacatcga aaaattcatt ccacgtcaaa ttacaaatat cttggcagga atcaaaccaa
    26761 aactttatgg tgaaggtaaa aacgtccgtg actggatcca cacaaatgac cattcaacag
    26821 gtgtctgggc tattttgact aagggtcgta ttggtgaaac ataccttatt ggtgccgacg
    26881 gtgagaaaaa caacaaggag gttcttgaac ttatccttga gaaaatgggt caaccaaaag
    26941 acgcttacga tcacgtaact gaccgtgctg gtcatgatct tcgttacgct atcgattcta
    27001 caaaattgcg tgaagaactc ggctgggaac cacaatttac aaacttttca gaaggtttgg
    27061 aagaaactat taaatggtat acagaaaatg agacatggtg gaaagcagaa aaagatgctg
    27121 tagaagccaa gtatgctaaa actcaagaag tcattaagta attgaaccaa aaaacctatc
    27181 attttgatga taggtttttt atgtacggct agttagatag ctaattggtc tgtttttgtc
    27241 atggtttgag gttggtaata gacattttga tagtcattac caactttttg tactaggtaa
    27301 agtcccgctt tattcatttt gaattgattg ttaatgagac tctcttgatt atcgtgtttg
    27361 ataacagccc aagtttgact gtttttatca tagataactt gttgttttga actattttct
    27421 agtaattcta cttctttgct gttggctaat ttatcaaaac ttgtgcgatc aatgtttgga
    27481 accatcatgt aaccatagct atcacctttg ttgtcatgct tttggcttat agtgataaaa
    27541 ggattgctaa cgattgaggt atttttagaa gtacgattaa tgctgttcca agtacctgtt
    27601 tgctctttgc gttcaatatc aatagtgcta tttttaaaga agatataacc aatattgcga
    27661 ccaggttctt ttgattctaa aaagacactt tttgtatctg taaattgttg agaacttgct
    27721 tgttttaaat caatagtttt tccattgacg tatgtagtat aaggtgtttt agaatcgtct
    27781 tttcgttgat caattgttgt agaaacattt ccaatgccgt tagtattctt gatgttgcta
    27841 cctaaaaaga caatcttatc atttaagata acccaacctt tttgtgctgt taaggtgcga
    27901 tcccagttag taaaatccat agcggcaaga gcaaagtgat cattaagttt gacggaacca
    27961 acaaagtcag atgttcctgt aacttgaccg gttttttctt tagcgtcttt gctatgtttg
    28021 ctcatgaatt ccttagtggt atcttcacgc ttagcatctt tttcagttgt tccagccatt
    28081 ttataaggat tgacggttgg ccaaaaatga ttactataat gagattgatc actattataa
    28141 atatagaaca taccatctcc ggtataccaa ccacgtgtat tttcatcatt cattccttca
    28201 tagttgaggg tacgtttaga atgtaatgaa agcgcgaaac caaagtcttt cttggcatta
    28261 taataagcta agcggtccat gctattaaag gttgataaat tactttttaa aggtttagta
    28321 gcgactgtac tgtcattaag cagcttattc atgttggcaa tatcggaata cgatttgagg
    28381 ttattgaaga cattgtagaa tttatttgaa gtgataatcg ttttaatagt tgatttgagg
    28441 tctaagtttc gctcttcatt agacatgtta gccaatctga ggaaacctct gagaacttca
    28501 actgcagccg catgcgaaga agctgcctct ctactaattg agcgtccacg actcatatcc
    28561 attaactcac cttttacaat taaaggtaaa aatgattgat taatccattt ataaaccata
    28621 tcaagttctt gattagagat tttatagtca gtttcttgaa tgataggcag caattgtgtc
    28681 aaaccatcta tcagaacatt accataggcg ccagtataag caacatttgt atggtcgatg
    28741 taagaaccgt cagcatagaa gccttcagct ttagtagcag tagtaaaaag attttttaga
    28801 gaatgagaag ttttttcgat aatagtattg tctttacgaa gtaaaccttc aatgatttta
    28861 acgcgcccca tatctactag attaccacca agggctttaa atggattgac aagcgtttta
    28921 cggaaatatt ctgcatcagg aacaaagtgt tcaattgggt cggtataagt ttttatttca
    28981 gcgtcagtga agtagttatt catgagagat agggtacctg taattgagcg aggaacaccg
    29041 atttcaaaat cccaccaatt ggcagagcct tctatatctt tattaacatt gtagaagttt
    29101 tgatgaagcc aagccagact ctcttttaca gtacgaatag ctttttcatt tttgtaaata
    29161 gtagagtgtg gattggtgat ttgtttagct aaatcttcca aacgacgata agtagcggtt
    29221 aactgtgctg aattattgag attatctaaa tctttccaaa ggaaagtgcg attagaatcc
    29281 agtttgatag cttcgatgtt tttggcgtta gtttcatcta atttctgatt aagcttttgc
    29341 atgttcgaat cattagtatc gtaaacataa ttaccaatag tcacgtcgtt ccatttgtcg
    29401 aggagttttg taaagttatc ttctgttgaa gctgtaactg ataacggtac ttctttaatt
    29461 attttgccag atttatcaga tattttaaca gttgttgaac cagtagcgtt tggatacaat
    29521 attccaccaa caattttccc aagagacgga ttggttagag tatattggta gtcagcttta
    29581 ttaaaaacgt aatttttgtt taaagccgta ttaacgcttt tttcaatttg tgttgtgacg
    29641 ggttgtggat gctctgagtc tttagggcct tttgttttca ttgatatatt atcaaaagta
    29701 acaaaaccag ttcctttttc ataataaagt tcaagtgtca cctcggagac atttaacttt
    29761 gggttatata tctttgttaa ggtatgtttg ttagtagtac cgctggtcat ctcagaaagc
    29821 caaagtcgcg tattgttgtc ttttttctcc ataatacgaa ggaaagcttg tccagccttg
    29881 ttacttgttt caatatcaaa cttaacctca tattttttag tgggatcaat gttcactttt
    29941 tgtgttacag cacctctaaa gcctttgtcg ctggatagac ttacttggcc ttgttcggta
    30001 cgttggataa taggtgattt atcagcagta tttcctttat caatccaagc agaccattcc
    30061 ctagcgcttg tatgtgacca gcttccagat acagggttag tttgattaaa atctccgttt
    30121 ttcaccagct ccttattttg agtttgatta tctactactt gtttcattgt gctatgagtg
    30181 gctgttgcag ttgcagttga agctgtgggg ttagacagat cttgttgtgt ggttgtggtg
    30241 ctagtctcct tgacagtggt actttcagtc tggagaactt caatattatt agtttgagta
    30301 tctcttattt gggacgttga ggtagttttg gtaagctctt cagctttagc ttggtaacta
    30361 ttgttaacca atattgttcc taaaataagg gctgaataaa gcataatacg atgtttcttt
    30421 ttgatttcca tttttactcc ttagggttta aaattgtaaa ccggtttttc ctaattatag
    30481 catgctttaa aggtttatga taaagaacct ttaaaatgtc atcaaagtga aaagtaaata
    30541 actggtaaac aaaataattt gtttaatcat ttgtgtgcta ctttgatagt aaattgccta
    30601 aataaaggct attttttgtt ataatatgag aatgagagga gtttgaaatg actaaattag
    30661 caacaatttg ttacattgac aatggcaagg aattacttct attgcatcgc aacaaaaaag
    30721 agaatgatgt tcatgaggga aagtggatct cggtaggcgg taaactggag gcaggtgaaa
    30781 ctccggatga atgtgctaag cgtgaaattc tagaagaaac ccatttaaca gtaaaaaaaa
    30841 tggattttaa aggagtcatc acttttcctg aatttacgcc aggtcatgat tggtatacct
    30901 atgtctttaa agtaacagat tatgaaggag agttaatttc agatgatgaa tcacgagaag
    30961 gaaccttaga atgggtacca tatgatcaag ttctctctaa gccaacgtgg caaggagatt
    31021 acgaaatttt taaatggatt ttagaagatg ttcctttctt ttctgctaag tttgtttatg
    31081 atgaacacca aaatttaatt gaaaagacgg ttaattttta tgaaaaatag agattttagg
    31141 gttagagacc agctcgaaac attcggagtc agagtttctg ctttaatcat agaaaatcaa
    31201 aaactgctat taatatatgc tcctcatctc tataaatact atttgccagg tggggcgctt
    31261 caagttggag aagatagcaa taaggctgtt gcgagagaag tacttgagga aataggtctt
    31321 cacagccaag ttggtgattt agcctatatt gttgagaatc agtttaatat taagagacat
    31381 cattatcact cagttgaatt tttatatttt gttaatctct tgggtcaggc gccagaatct
    31441 atcaaagaag gaacgcataa gcgacatttt gtatggcttc caataaaaga attaacaaaa
    31501 attgattgta atcctaattt tttagcacag gatttgatag aatggccagg tcatgttgtg
    31561 cataaaatcg ttcaaaatta actttttact ctcttgtttt gagtttaatt taattcaacg
    31621 tgttataata aatgagaatt aaaattagaa ttcatcttga aaggatatat gaatgaatca
    31681 accatcagaa aaagaattta aaaatagttt gttttttaaa tggatcttaa ataatcaagc
    31741 agttattgct ctcatgatta cctttttggt atttttaacg atttttattt ttaccaaaat
    31801 ctcttttatg tttaaacctg tgtttgattt tcttgctgtg ctgatattgc cgcttgtaat
    31861 ttctggcttg ctttattacc tattaaaacc tatggttaca tttttagaga agcggggaat
    31921 taagcgtgta acagcgatat tatcagtttt tactattata atccttctgt taatttgggc
    31981 aatgtctagt tttattccca tgatgagtaa tcaattacgc cattttatgg aagatctccc
    32041 ttcatatgtg aataaagtgc aaatggaaac aagttcgttt atagatcaca acccttggtt
    32101 aaaatcttat aaaggggaaa tatcgagcat gttatctaat atcagtagcc aagcggtctc
    32161 ttatgctgaa aaattttcaa agaatgtttt agattgggca ggaaatttag ctagtacagt
    32221 tgcacgtgtg acagtagcaa caatcatggc tccctttatt ttgttttatc ttttaagaga
    32281 tagtcgcaac atgaagaatg gtttcttaat ggttttacca accaaactac gccaaccagc
    32341 tgatcgtatt ttgcgagaaa tgaatagtca aatgtcagga tatgtgcaag gacaaatcat
    32401 tgttgctatt actgttggtg ttattttttc aataatgtat agtattatag gccttagata
    32461 tggcgtgaca ttagggatta ttgccggtgt gttaaatatg gttccctatt tgggaagttt
    32521 tgtcgcccaa attccagtgt ttatcttagc gcttgtcgca ggacctgtta tggttgttaa
    32581 agttgcgatt gtttttgtta ttgagcaaac tctagaggga cgctttgtct cacctttggt
    32641 tttaggtaat aaacttagca ttcatccaat tacaattatg tttattttat taacctctgg
    32701 agcgatgttt ggtgtttggg gagtattcct cagtattccg atttatgcat ctatcaaagt
    32761 tgttgttaaa gaattgtttg attggtacaa agctgtcagt gggctatata caatagatgt
    32821 tgttactgaa gaaagaagtg aagaagttaa aaatgttgaa tagtgaaaaa atgattgttt
    32881 ctatccaaaa tcaggattta gaacatgcta acaagtattt cgaaaaagcg ttgaaaaatg
    32941 atcctgagga aatcttattg gaactaggag cttatttgga gagtattggt tttttacccc
    33001 aagcaaaacg tctctatgat caaatccgac caaattatcc agaagttgct atcaatttag
    33061 ctcaaattgt tgccgaagat ggagatattg aacaagcatt cttatactta gattatatct
    33121 cagaagatag tcaagagtat gtttcagccc tcctcgttat ggctgactta tatgatatgg
    33181 aaggcttgac tgatgttgcg cgtgaaaagc ttttgctagc cagcaaactt agcgatgacc
    33241 cattggttac atttggttta gctgaaatga accttagttt ggaacattat caagaagcga
    33301 tagaaggata tgcatcactt gacaatcgag aaatcttaga aacgactgga gtttcaactt
    33361 atcagcgtat tggtaaatcc tacgctatta tagggaaatt tgatgcagct atcgaatttt
    33421 tagaaaaagc cgttgacatt gagtatgatg atttaacagt ttttgagtta gcaacaatcc
    33481 tttatgatca agaagaatat caaaaagcta acctttattt taaacagttg gatactatta
    33541 atccagactt tgatggctat gaatatatct atggtttatc tcttcgtgag gagcataaat
    33601 ctgaagaagc tttacgtcta gttcagcaag gtcttcgtaa aaatagtttt gatggtcaac
    33661 ttctcttact agcttcacaa ttatcgtatg aattacatga tgttccttcg tcggagtctt
    33721 atttgaaaca agctgaaaaa gtttctgaaa accaagatga aattgttatg agattgtcaa
    33781 atttgtatct tgaagaagag cggtttgagg aagtcttgga attagacaat gatgatttag
    33841 aaaatatcct tgcaaaatgg aatatcgcta aagcacacaa ggctttagaa atggatgata
    33901 gtgtagacta ttatcaatct ttgtataatg atttaaaaga taatccagaa tttttacaag
    33961 attatgctta cattttgaga gaatttggtt accttgataa ggcacaagaa gtaggaaagg
    34021 cttatttaaa attagttcca gacgatatag agatgtcaga atgggtgaat aacatttaaa
    34081 taggaaaaca cttacagaaa aaaataaaaa attcacaagt aaattttcac aatctgatgt
    34141 aaaaactgtg atataattga accattcttg tgaaaagagg gagataaaat gacagaatca
    34201 cataatcaat atggggctga cttaattgtc gatagcctca ttaatcatga tgtcaaatat
    34261 gtttttggta tcccaggagc taagattgac cgcgtatttg acactctaga agataaaggt
    34321 ccagagttaa ttgttgctcg tcatgagcag aacgcgactt ttatggctca ggctgtcggg
    34381 cgtattacgg gagaaccagg agttgttatt gcaactagtg gacctgggat ctcgaacttg
    34441 gcaacagggt tagttaccgc gacagatgaa ggtgatgctg ttcttgcaat tggtggacag
    34501 gttaagcgag gagatttatt gaaacgtgct catcaatcaa tgaataacgt tgctatgcta
    34561 gaacctatta caaaatattc tgctgaggtt catgacccta atactttgtc agaaactgtt
    34621 gcaaacgcct atcgtcttgc taaatctggt aaaccaggag caagttttat ctctattcct
    34681 caagacgtta cagacagtcc agtctcagta aaagcaatca aacctcttag tgcacctaaa
    34741 ttgggttcgg catctgtttt agatattaat tatttagcgc aagctattaa caatgctgtt
    34801 ttaccggtac tgttattagg aaatggcgca tcttcagagg gagtaactgc tgccgtacgt
    34861 cgacttcttg atgctgttaa actaccagta gtagaaactt tccaaggtgc gggtattgtg
    34921 tcacgtgagc tcgaggatga aacattcttt ggtcgtgtag gcctcttccg taatcaacct
    34981 ggagatatgc ttttgaagcg tgcagaccta gttattgcta tcgggtatga ccctattgaa
    35041 tatgaggcgc gtaactggaa tgctgaaatt tctgctcgta ttatcgttat tgacgttgaa
    35101 caagcagaaa ttgatacgta tttccaaccc gagcgagaac tgattgggga catggcacat
    35161 acacttgatt tacttttacc agctattaaa ggatatgaat tgccagaagg ttcaaaagag
    35221 tatttgaaag gtcttcgtaa taacattgaa aacgttagcg atgttaaatt tgatcgtgac
    35281 tcagctcatg gacttgttca tcctcttgat ctcattgatg tactccaaga taataccacc
    35341 gatgacatga ctgttacagt tgatgtggga agccattata tttggatggc tcgctacttt
    35401 aaatcatatg aagcgcgtca cttgctcttc tcaaacggaa tgcaaacatt aggagttgct
    35461 ctaccttggg caatttctgc ggctcttttg cgtcccaaca caaaagttat ttcagtttct
    35521 ggtgatggtg gcttcttatt ctcagctcaa gagttagaaa ctgcagttcg tcttcatcta
    35581 ccaattgttc acattatttg gaacgatgga aaatataaca tggttgaatt ccaggaagaa
    35641 atgaagtatg ggcgttcttc aggtgttgac tttggaccag ttgattttgt aaaatacgct
    35701 gaaagtttcg gtgctaaagg ttaccgtgta gacagcaaag acagttttga agaaacactc
    35761 aaacaagctc taatcgatgc tgaaaatgga ccagtgttga ttgatgtgcc tattgactat
    35821 aaagataacg tgacactagg tgaaacaatc ttacctgacg agttctacta agacgaggtc
    35881 taatatgtca gaaacagtta aattatttca atacagcacc ttgagttcac tgatggcagg
    35941 attgtacaaa ggcagtctaa ctatcggaga attattaact cacggtgact taggaatcgg
    36001 tactgttcat atgattgatg gtgaattgat tgtattggat gggaaagctt atcaagctat
    36061 tggtacagat ggtaaagcag agattatcca attatcagat gatgtgacag taccctatgc
    36121 agcggtttta ccacaccata tccaaaaaca atttgatatc aatgctgaga cagataataa
    36181 ggatttggaa gagatgattc taaaaaactt tgaaggtcaa aatctcttta agtctttaaa
    36241 aatcaaagga acgttttcaa gaatgcacgt tcgtatgatt ccaaaatctc cacaacataa
    36301 acgttttgca gatattgcat ctaatcagcc agaatttacc agagagaatg tatctggtac
    36361 gttggttggg atttggacgc cggaattatt tcatggtgtg ggggtaaaag ggttccatgt
    36421 ccactttatt tcagacgatt taactttcgg tgggcatgtt atggattact cgctcacaca
    36481 aggaaaagtt gaaattggaa aggttgacca acttgaccaa tgtttcccaa ctcaagatca
    36541 agaatttttg aaagctaact ttgacctaca aaagcttaga gaagatattg atttaagtga
    36601 ataagaatac ctataaataa ttaataacac taaaaataga cagggatatg tcccagtcta
    36661 tttttttagt ttaaggctat ctattttttc ttgtgtggga gtgactcgta aggttttttg
    36721 accagtgtaa gttacaaaac ctggcttagt accacttggt ttattgagtt tttttgcttc
    36781 aatcatgtct acttgaacaa gattggagag acgagcttta gagtaataag cagctaactc
    36841 ggcggcatct gtcttaacct cgtcacttgg attgaggtta tcccgaatta acacatggct
    36901 tccggggata tccttggcat gaaaccacaa ctccccttta cgagccattt taaatgttaa
    36961 ctcgtcattt tggagattat tacgtcccac cataataata gtcttgccat cggatgctaa
    37021 atattgttca ggttttttac gcttatgttg tttatcatgt gcgcgtcgct taataaatcc
    37081 agtttcaaca agttcttcac gaatatcatt gatatcttcc atagaagcat gatttagcga
    37141 tgtttcaaca gattcaaggt aggtgattgt atttttcgta tctgaaataa ttccttttaa
    37201 atgttttaca gcttctttta atttttgata ttttttaaag tagcgttggg cattttggtt
    37261 gggtgttaaa gctcgatcaa gtgaaatttc aatcgtttgg ttggtgtaat aattatcaag
    37321 aacaacgata tcttgattat ttggcaccat agagagatag gtagttagaa gctctccttt
    37381 ttggcgaaac tcctctgcat tttcagtagc tagaagttcg tcttgttgct tagctagttt
    37441 tttgatattt ttctctaact cactttgaac acggtgaatg aggtcactag cttgctgtgc
    37501 tatgcgatct ttttcagcct tctcttggta gtaataatct aacaattgtc caagagactg
    37561 aaatgtttcg tgactgcttg agaattggac agcggaaaac gatttttctg ttaggctagg
    37621 ataatattct ctcgaaaaga attgacgaaa atcgttaagt ttattgtctt tcaaacagtg
    37681 agataattct agcgcagtat cgcgtcctaa accttggaag agctgttgga ggtttttagg
    37741 gcttaggtca ttggtttgaa gcagctcaaa tagagtttga tcagaaatat caaaaggatt
    37801 gattgctttt gttttgggtg gggcaatgta agttgaacca ggaagaatag tacggtagga
    37861 gttttgcgag aaaccaacat gcttaattga ttcaataatt ttatgttcat ttttatcaat
    37921 caagataatg ttactgtgtt ttcccatgat ttcaacaaca agtgtcgctt taatgtgatc
    37981 accgatttcg tttttattag acacgatgat ttctaatata cggtcatttt caatttgttg
    38041 aatggtttca atcactgcac cttgcaaata ctttctcatg atcattgtaa aagtatttgg
    38101 attttgagga ttttggaaat ttgcttcggt agtttggata cgtccaaata cagggtgagc
    38161 agataggagt agtttatagt tgcgacgatt attgcgtatt gttaatacca attcatggtc
    38221 aaaaggttgg ttaacttttt ggatacgtcc tttttcaatc tgttcttgta attcatttgt
    38281 gaggtgatgt aaaaaaaatc catcaaaaga catggcgtcc cccttattct aaaaattata
    38341 gcttatttat tataccatag gctgagctaa aaatattttt ttataacgca aaaggcttgc
    38401 aattttcaga atatagtata taattataga aaaattcatg agaaaggatg actagtaaat
    38461 gactaaaaca ttatttacca attggcaaaa ctggcgtaga taaagttgtg tttatgggtg
    38521 catagatacg actttttgga gtacttcaaa aaagtatcta tgcgctggtc tagtctaaca
    38581 atagagagcg caaggtaagt tatttatcag gcgctttttc ttttgtgcta aactccagaa
    38641 agtcaggata ttattaaagt gaggagaaaa tatgaaaaat aaaggactga tagctacgct
    38701 aattctattg accatattag tggttgggga attattttat aacaaatcag aaaaacatct
    38761 taatttatca gaaaagcaag ttgttaaaat tggtatttta cagtatgtaa cccatgatgc
    38821 tttggatgct attgaaaagg gagttgaaga tggtctagct caagaaggat ataaagggaa
    38881 aaaagttaaa ttaaccgttt taaacgccga agctgatcaa agtaaaattc aagcgatgag
    38941 taaacaatta gttaatcatc acaatgatat tttgattgga attgcgacac catcagcaca
    39001 aggtttagca gcttcgacta aggatactcc tataatcatg ggagctgttt ctgatccttt
    39061 aggggccaag ttagtaacga atatgaagaa accaacaact aatgttacag gattatcaaa
    39121 cgttgttccg accaagcaaa ctgtgcaatt aatcaaggat ataactccca atgttaaacg
    39181 tataggaatt ctttacgcta gtagcgaaga taattctgtg tcacaagtaa ctgagtttac
    39241 aaaatatgct caaaaagcag ggctagaagt tcttaaatat tctgtaccat ctacaaatga
    39301 aattaaaaca agtatgtcag tcatgaccaa aaaagtagac gctgtctttg ttccacaaga
    39361 taatactatt gcgagtgctt ttagaacagt tatcgtagca gcaaaccaag ctaatatacc
    39421 tgtgtattcc agtgttgata ccatggttga acaaggaagt atagcatcag tagcgcagag
    39481 tcaatatggc ttagggttag aaactgcaaa acaggccatt aaagtcctaa gagggaagcc
    39541 ggttaaagat gtaccagtca aagttattga tacagggaag ccaagcctca atttgaaagc
    39601 agccaaacac ttaggaatta aaattcctaa gaagattatg aaacaggcag aaattaccgt
    39661 taaggttgac gattagaaag agggttttat gattatttca tcagtttcac aaggtttatt
    39721 atgggggata ttaggattag ggatatactt aacctttaga atcttaaaat ttccagacat
    39781 gacaacagag ggctccttcc ctctaggggg agcggtttgt gttaccttaa tgaatcaagg
    39841 ggttaatcct attttggcga cgattctagg catgctatca gggatgttag cgggatttgt
    39901 tactggttta ctttatacta aaggaaagat accgacgatt ttagcaggga ttttagtaat
    39961 gacctcatgc cactctatta tgctaatggt tatgaaaaga gcaaatctag gtttaaatga
    40021 gattcaaaca ttgaaagatt ttcttccgtt ttcaaatgat ttaaatcttc tcgtattagg
    40081 tttgatagct attctcttgg cgatttctgt cttaatttat tttctctata cacgcttggg
    40141 gcaagcttat attgcaacag gagacaaccc tgatatggca aagagcttcg gcattgatac
    40201 cgacaaaatg gaaatgcttg gcttaattgt atcaaatggc ttaattgctt tatcaggagc
    40261 tttagtaagt caacaagatg gatatgctga tgtatcaaaa ggtataggtg ttattgttat
    40321 tgggttagct agtattatta ttggagaggt actttactct actggtttaa ctctctttga
    40381 acgtttaatt gctatagtgg tgggttctat tctttatcaa tttttaataa cggcagtaat
    40441 cgccttagga tttaatacga attacctaaa attatttagt gcaatagtgc ttggaatttg
    40501 tttaatggtt cctgtcctaa aaacgaagat tttgaaagga gtacgcttat aatgatgatg
    40561 aaaatcatag agttaaaaga agcaaccgta caagtcagta atggtttagc agaaatgaaa
    40621 acgatattag atcatgttaa tttgagtatt tatgaacatg acttcattac aatattgggt
    40681 ggaaatggag ctggaaaatc aacgcttttt aatgtaattg caggtacctt gatgttaagc
    40741 agtggaaata tttacatcat gggacaagat gttactaatt tatcagcaga aaaacgagct
    40801 aaatatttat cacgggtatt tcaagatccg aaaatgggaa cagcacctag gatgacagtt
    40861 gctgaaaatt tattagttgc taaatttcga ggtgaaaaga gaccgttagt tcctagaaag
    40921 attactaact ataccgaaga atttcaaaaa ttgattgcta gaactggtaa tggacttgat
    40981 cgccatttag agacacctac aggtttatta tcaggtggac aaagacaagc gcttagttta
    41041 ttaatggcaa ctttgaaaaa accaaatctg ttattattgg acgaacacac agcagcgctt
    41101 gatcctagaa caagtgtttc gcttatgggc ttgactgatg agtttatcaa acaagattcg
    41161 ttgactgcct taatgattac acatcatatg gaagatgctc tcaaatatgg aaatcgtgta
    41221 ttagtaatga aagatggcaa gattgtcaga gatttaaatc aggcacaaaa aaacaagatg
    41281 gctatagctg actattatca attatttgat taaaaagaag tgaaatggaa gtgtttcact
    41341 tctttt
//
LOCUS       DATRME010000009        39806 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRME010000009 DATRME010000000
VERSION     DATRME010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39806
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..243)
                     /locus_tag="VBC23_000496"
     CDS             complement(<1..243)
                     /locus_tag="VBC23_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842469.1"
                     /translation="MEAIIDLHGCHVNPKETVMTALNQIINEIEEELSILEDNYEDTR
                     YEEIQAITDSEKEELIETELELSSQFDGVKDELRDFF"
     gene            complement(264..575)
                     /locus_tag="VBC23_000497"
     CDS             complement(264..575)
                     /locus_tag="VBC23_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842470.1"
                     /translation="MKIEIYCTKATFKLVDSYELSSNKFESFKELIFRLTWGDYAKGG
                     LHTAYVIDKEKNIDYKLDAVMLSGVVDDEDERGLFDMLLKAESNQSIQEFSTYYLELE
                     R"
     gene            complement(579..944)
                     /locus_tag="VBC23_000498"
     CDS             complement(579..944)
                     /locus_tag="VBC23_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842471.1"
                     /translation="MTSIQVKFDVVSSMNLENLQSLIETISRRYQLIHLYLADFNQRT
                     NDCEITLVISSQDNNVKNFSDLQDLLRQCLKGTSELDQIEDDFDNQNIKTLQEAWKII
                     IDDLAENIIEWIEEEFEGE"
     gene            complement(1053..1637)
                     /locus_tag="VBC23_000499"
     CDS             complement(1053..1637)
                     /locus_tag="VBC23_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO6842472.1"
                     /translation="MVKIGYARVSSADQNLDRQLKQLNNVEKIFKESVSGASRERPQL
                     IAMLDYIRDGDIVVVTELERLGRNNKELTEVMDEIKLKGATLEVLNLPTLRGIEDDNL
                     RRLLNNLILELYKYQAQAERERIKERQAQGIAIAKAQGKYKGRRALFSADDSRLQHAF
                     GLFLSGKSDRDVAQLTGINERTFRRYREKYGIHR"
     gene            complement(1744..3054)
                     /locus_tag="VBC23_000500"
     CDS             complement(1744..3054)
                     /locus_tag="VBC23_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HEO6842473.1"
                     /translation="MSIWERYSKEEREEYIKFLKVYGALTNLFRQKHENLIPYLDSKF
                     QETIYAKVFNSENVDIGNTPHDILSVFGDDRIGIGLKTWMNSRPSFQKVMQLKRYKAD
                     IDPYFNKSDEELALKLSELKNERMLIDYNRLGLAKDKNIYHYVTRDEGKFVIQETLYP
                     LVDLNKLGKFKRTNTAFTWSDGHKTYKYTYGDSQIWQYFNSDADDTLVLNQFDVNIIE
                     DPFDFLLKSYFSFVDDFKKAENSDDIVEVYLPLYSYRTKEVELKSGLNAWNAALKNKN
                     SATLRPLNEVYIPIPLEFHKKYPDFFCSDVFEIQRRQKNYVGNKKNKPQVRFHLQLPN
                     GKKIPALLTQSDMKSLQSGSLTEKDPNTGKLYGQSALGQWLLVDVLGLKERQPVTREW
                     LEKKGTDAVRLWHKKDDYNTINIDFAPIGSFETFMKDEINNSDD"
     gene            complement(3129..4349)
                     /locus_tag="VBC23_000501"
     CDS             complement(3129..4349)
                     /locus_tag="VBC23_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO6842474.1"
                     /translation="MTSSVSSQQSKKYNLAGFFSGVGGIELGFTQTNEFRVVYANEFD
                     KYARMTYKLNYPDTNLDSRDIHAVDPKEIGQENIDIIVGGFPCQAFSIAGYRKGFEDD
                     RGDLFFELLRMIEDRKPRAIFIENVKNLVSHDHGNTFKVIREALTENNYFIKWKVLNG
                     KDYGNVPQKRERIYVVGFNNKTAYDLFEFPNEINLTTSLRDVIDFTENKDEIFYYREG
                     KQKFYSELEQVMTKQDTVYQWRRQYVRENKSCVVPTLTANMGTGGHNVPLILTDNGDI
                     RKLTPKETFNVQGYPKSFKVPENVSNGQLYKQAGNSVVVPVIKRIATNVAKALNNSTT
                     AKSEQSTGKVAIIYTKINGQFEGASYVKEFVDSYEHALARLESYQDNLPILSDEEYLK
                     IIKRKSKREFYSIV"
     gene            complement(4425..6059)
                     /locus_tag="VBC23_000502"
     CDS             complement(4425..6059)
                     /locus_tag="VBC23_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO6842475.1"
                     /translation="MVVTKVLQIKTSRNLKRAIDYITRDNATLKLDTEHLEGDENYSY
                     EIVDGQVMKRLVSGHDLTDISDPQTIYEDFILLKQSVDVLYNNETLSDLKNDQRVLAH
                     HIIQSFSPEDGLTPEQVNEIGRKTALELTGGDYQFVVATHMDKGHLHNHIIFNTTNEV
                     TLKKFRWQKNTARNLFQISNKYAELYGAKVLEPRLRNSHTEYAAWRRKNNYRFEIKER
                     LNFLLKHSLDMNDFLQKAKALNLQIDTSGKYVKYMLMDQPQERFVRDRTLSKKGKFSL
                     EKIKEQIATNEVVYDLNVIKEKYDEEQESKQDDFELQVTIEPWQVQQLTSQSIYVPIT
                     FGLDRKGTVSIPARMLDQNEDGTFTAFVKKNDFFYFLNADHSEQNRFINGTTLIKQLS
                     AQNGEMILTKNKNITNLDRLVDEFNFLAINKVTNSKQFEELQTQFLKQLEETDKTLDV
                     LDEKMTYLNKLMGALSDYQNNIVPSEVSLELLETGKINKTTKLEELQKEIKELQIERD
                     TLKKHRDKIVKDYDFAKEMKEEHEKAKYKNSNKIKI"
     gene            complement(6031..6405)
                     /gene="mobC"
                     /locus_tag="VBC23_000503"
     CDS             complement(6031..6405)
                     /gene="mobC"
                     /locus_tag="VBC23_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO6842476.1"
                     /translation="MADKKRKRSVEFKLRANEEEAAKIRQRFSRANKKTFQAYALEML
                     QNGKIATYDYSELQKLRIEVNRIGQNVNQLVRYVNTFDEIDNELFKALQAEIDDMKKL
                     LFDEFKTKGRAKLNGSDESSSD"
     gene            complement(6719..7012)
                     /locus_tag="VBC23_000504"
     CDS             complement(6719..7012)
                     /locus_tag="VBC23_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842477.1"
                     /translation="MITEEMMMITEMLLMSYYFDMSEWLKGVKTVNIDIIQKSKDDLL
                     DMLKSDFEELSTEDNNEYLDDLSVCIATLEELSEDAYQNIKTEVFSWEPGQKN"
     gene            complement(7070..11707)
                     /locus_tag="VBC23_000505"
     CDS             complement(7070..11707)
                     /locus_tag="VBC23_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEO6842478.1"
                     /translation="MANMQELLSQRFEARKQKLVSMDIVAVAESLGMKLKPGSSGAYY
                     WEEHDSFHIYPNTNSFCWWSRSVGSNTIDLVQVVREELTGHKPSFKEAAAFLETGHFE
                     NVTVQPPVREPFEYYLERYEHSDFNIGRQYLKEERGLSDDTINTFLASGNLASATRKK
                     GDYFEPVIVFKSRDNDGKMIGASLQGIVENRVQHSERGRLKQIMKNSDGTAGFSLDIG
                     NPKRLVFAEAPIDLMSYYELHKDELQDVKLVAMDGLKKSVISRYVADMLTDGKYSQTM
                     SNEQIRGALDALNKTTNILQEHPDLITLAVDNDEAGQNFIKGLQEDGIPVVSDLPPRQ
                     ANQIKMDWNDYLKQETNPKENIANKAWDIDDIQEQLSQESELVGTQTVDVVYMNEFYL
                     NQGYSLSLAVHSNEEVENLADDVAPWTLEVMKNGQSLGFLAYGEDWGNAFEIEAELAN
                     LEKWVKENQVSNHLYSQKDVNAFLATIQAVEPKRGEFVLKGGGIYDFGESQEEGEELR
                     QRALDGMIADITDGETYYLWYDEELENLNAPDEAFINFHYHLKDIEYNQDNIHLYVAD
                     STTDGVTGYLSLDGDSIEPTALEDYLTEQNWTFDQKVLFLKNLKTAIDETWDKVINHY
                     DEQFNAIVAQYGLSSAKEKAPELLQETEKANFEQQKKVENPIGDLPEQSQEAAPLPST
                     REEYSLNESSPTHSQSQPLLHFTISEPEKSIYKIGYHPVNKKELNKLNRYAAQIQENA
                     NWYKNELADSNVTYFYKNNDNLEALKVTFKAEQYPHLVGIFAIDDDQSASKTLDDLAS
                     GKGDYQNIMVANRGATFSKIQVLPDFKAVVDSNSFIFDDLSEVERMNRLDLAKAIRTE
                     DKDVLIAFRNVDGEYLPASLMKVTNKLESELSMANNKDVLGIIGEKDGEFKVLSVNEE
                     VIKDGGEKMLSIVKNGQLEDLSNTVQKAEWVVEFNEANLSGNVEIPNFTGAGLTEELL
                     ERIKDYDYRLYLASLNDEGEGYYKFYFNKVQDGKVVKQVRLDVGDGLERNEEIYKFLE
                     QELAPEKAVKVVEKELTAEDFTKVLDAVYNVGAQVGKDNRTNIPEELYPAWDKYYEYA
                     ERYDNNFDVIVKAARADNLFDENSDFYKDVWLRKIQEENQKVKDSDGDGLTDDEEIAL
                     GTNPYSPDTDGDGILDSVEKASGTDATNPSDTPDSRKEEMNKRDLTLSEMIKAKNTAA
                     LNQHLQEGIKDYFDSDNYKNFLEGMSHFNNYSVRNIQLIKAQLPEASMVASFNEWKKR
                     NGHVNKGEKALYVQAPVTVIKKDVDGNPIINSETGEKETLTYFKPVPVFDISQVSPVK
                     GKELNLPKMGEVIPSQITKEYYQNVYRSLRDISQKENGIPIRFRELEKSDGSYSRKTN
                     EITIKKGMTYEQTLSTLIHEMAHSELHNKKSLTERFEGKLTRSSKELQAESIAYVVSN
                     HLGFDTSNDSFAYLASWSKEPDGLENLKAQLEIVQEEASSLMKRIDKHLEKYQNLTVS
                     KDEKLNENQKRELEKTANPFYQSLQKAKGETVQTAPAKEEQSKKVNRPMKQ"
     gene            complement(11761..13497)
                     /locus_tag="VBC23_000506"
     CDS             complement(11761..13497)
                     /locus_tag="VBC23_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEO6842479.1"
                     /translation="MKYLLLAEKPDQAKKYANALGNPKNDKGVWRVQSSVLNAEVIVA
                     PAVGHLVERINPYTNFENWDMANLPALPDGFSYEPKKDTAKRFKAIKQAVKEVDAIII
                     GTDPDREGEAIAYRILELIPGALRKIKYRLWANSLTKKGLEQAFSKLKNPKDSINYFH
                     EADARGDADWLVGFNLSPFVTIKMKEEGYLDKKDHSMSVGRVQTPIVSLIVRNDEAIE
                     NFKPSPYWQLKLIDPEAKVTFSNDTKYQSKAEAEQMLQQLANIAVVKTVTNEEKVQEA
                     PKLYNLTNFQSEMSKLYHFDATKTKELVQSLYQKGYLSYPRTDSTLITTNEFAYLVEH
                     IDDYQIAIHRQLETPNRSPRKNYVNDKKVLEHYAIIPTENIPDLNELSDDEKLIYQKV
                     VFRTLLMVTPDYRYQSTSVILDNRGIEFKATGAVTKDKGWRNYLAVKKEDRELPDYQE
                     GKNLVVDCKLLEEMTKPPTRITEQILLKKLLPKYNLGTSATRDGMIDLIQDKGYVTKN
                     KKTGQFFPTERGKLLIHYLDQLEIAYTNPETTGKWEEILAQIGQGKIHKEAFVNKIKW
                     AITKQIEKGKQL"
     gene            complement(14089..14409)
                     /locus_tag="VBC23_000507"
     CDS             complement(14089..14409)
                     /locus_tag="VBC23_000507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842480.1"
                     /translation="MKLFKRKHPIKQELKPEIDVENLIGIGYVRHPNFDNMYRQYTSE
                     QLEELIASLKETIRFLEYQKSLSVAFHEGQTQEVQHLLENKIEGNLSYLRKDEGWLLR
                     ILKR"
     gene            complement(14428..14862)
                     /locus_tag="VBC23_000508"
     CDS             complement(14428..14862)
                     /locus_tag="VBC23_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842481.1"
                     /translation="MRALKKLIEGNLSSLGLIELIDDRHDKFGGYYLLYSLSLTAITP
                     EFSESGTLDIEVSFNDYQELTYELGKYSTPFYVNSKDELKCLLEGIVDLPLPFSVSYK
                     PIKEVTWDRYIVSIDGVETTRIIELVKMIKVLEDYLSQVLRG"
     gene            complement(14939..15082)
                     /locus_tag="VBC23_000509"
     CDS             complement(14939..15082)
                     /locus_tag="VBC23_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842482.1"
                     /translation="MATSKQSPFFKTLGISEDELLHYLKEHPDDPLVTKLEQLLLNLS
                     DKK"
     gene            complement(15118..17184)
                     /locus_tag="VBC23_000510"
     CDS             complement(15118..17184)
                     /locus_tag="VBC23_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO6842483.1"
                     /translation="MKLQKKPLIPYLIISLVLAFLTHRAYALYSIAPKPDMTNLFSQY
                     TFVLEKYVEPPYFYPNFSPFAILAAMVGFFIGMMFYLKIKPDENYRHGEEAGSAKFAI
                     AQEIAGFKDPEPTNNMIFTKNAQMGLFNKNLPFQWQLNKNVLVVGLPGDGKTFTYVKP
                     NLMQMNSSFVVTDPKGLLVRETGKMLEEHGYQVKVFDLVNLTNSDMFNPFNYMKSELD
                     IDRITEALVEGASENEHKGEPFWDKAQLLLNRALIGYLYFDSKTRDYIPNLSMVAELL
                     RNIRRPNDKEPSTVEKMFVELEESMPGNYACKQWNLFNDNFDAETRTSVFAMVATQYS
                     VFDHQAVTDLIKSDTMEMDTWNTEKTAVFVAISETNKAFSFLASTLFTVMFDQLTHNA
                     DAIIQGQKDGYTADDLVHVQFIFDEFANIGKIPHFNEVLASIRSREMSVKIIIQAISQ
                     LDTIYGDKARKSMVNNCATLLFLGTNDEDTMRYFSMRAGKQTITQKSYSEQRGQRVSG
                     STSTQAHQRDLMTPDEIARIGVDEALVFISKQNVFRDKKAMVTDHPMKDELSNNPNDE
                     KWYDYTRFMVEDPELFNNARDGKIDPKNIWYPDMKGYDQFVKDNDIDTSKLTEIEAAQ
                     ADEASTQATEEPVYEQVPVQLPEMTVESQSQTQESEIKAVDFATGEIFELPPEDEEDD
                     EYYGEV"
     gene            complement(17184..17675)
                     /locus_tag="VBC23_000511"
     CDS             complement(17184..17675)
                     /locus_tag="VBC23_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3801 domain-containing protein"
                     /protein_id="HEO6842484.1"
                     /translation="MEQEQILDRGARVTLETGKQLFHFLAYAAQQLYKAYDEQKLVGQ
                     QSWKNFNQSSLTKDHIDFLEADVNLKKFTDELKKSGVRFAFKDLGDGTKQVWFEAPNR
                     EVIADALRNTLNEIINDPKKAKEKYMKSEKELTPKEQINKIKKNTKEKLDTVQTKKKG
                     KSI"
     gene            complement(17690..18499)
                     /locus_tag="VBC23_000512"
     CDS             complement(17690..18499)
                     /locus_tag="VBC23_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842485.1"
                     /translation="MFRITNLSNGQQRDFQNREELEFYLEGEENRCLQLNTTATFQLF
                     HLDKNGELLESMELTIPSSGDQDVKELLGEFGLKKDKKFFWSRGSKQVNVQKVAEKPT
                     KKIKAKNKLTKPTSSARVTHTSTSHHHFWTMIALLFSIFSLAISLTNHYNNKVVDKPK
                     IEKTTINQGADVFCRYFISNYFANSSAREDFISNSLNLNQFDTDKATTVSVLLEKEVM
                     TKKVTTLTYVINCRYDDNTTANKRLTLTVKQNKEAKYGYLVSKAPELTAYP"
     gene            complement(18499..18774)
                     /locus_tag="VBC23_000513"
     CDS             complement(18499..18774)
                     /locus_tag="VBC23_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842486.1"
                     /translation="MNETTFGTEQHTSKSARRQKSTSDRLKDKLKQLNKAKASLEAAQ
                     KKIKQLEADIKELEAKRQQEILKEYGMSLSDLEAFLADNKDKLGGDA"
     gene            complement(18790..19176)
                     /gene="ssb"
                     /locus_tag="VBC23_000514"
     CDS             complement(18790..19176)
                     /gene="ssb"
                     /locus_tag="VBC23_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6842487.1"
                     /translation="MLNQSIITGRLTKNTELRKVNDRSVISFTLASSRDYKTEDGSYP
                     ADFIDCVLWGAPAESFNTLTAKGDTLQVTGRLQTRTYKDKKEVTHKVTEVQVDKWYIV
                     AVNQNQGDYPVKEKTIVNDSLDNLSL"
     gene            complement(19188..19793)
                     /locus_tag="VBC23_000515"
     CDS             complement(19188..19793)
                     /locus_tag="VBC23_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842488.1"
                     /translation="MIDEFKVTFFKRVMMIVGIIIIFLIGLGFGSMSKKTDNQSSSHT
                     TLTKKVKEGHQLTEGWVKQFLIAYYTKKDLEENRNRYKDYMTDGMYNAAVSEEETAQN
                     QAYKGFVVNFEFKDAQIYIDKTNDKVICYVNYTNDLLQKKNSTEGAQTGVDNNTIIQL
                     TYNKVDGKYLVNNMSTLLITDSQDPTASQNSYGDVIASNDE"
     gene            complement(19810..22473)
                     /locus_tag="VBC23_000516"
     CDS             complement(19810..22473)
                     /locus_tag="VBC23_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6842489.1"
                     /translation="MSNKDDLRKRVVRKRHIKESVQRHNQLSAKKAQNAAKKEHREAK
                     IKVKHARAALRRAEKGIDKDVDGEKIVDDLEKALAEKKATKKIKKKVRKTNPTIGQKA
                     RRTGKNIALNASHQIKSTAMDQDDTLSDLQNLQYRTRQSKAVAKQAGKVTWRGAKLAY
                     RKSKGMVNVSRGAYRSAKDSAKFATKTTAKVATKVAQTTASAVKTATSAAATILANPM
                     TWIVSAVVGFTLFLVILVSSIFSSNVIQQNDFTLNQSWLHISQVDRQKSSDQVEYYTD
                     IDSILLYMNFRYGGEWEPDANWDDGTGGKLTGALGFNHFSDALDDIWKEENSDIDNLK
                     TMAELYTNGKAWMKLDKDDLADYKEILESQSETGKYLATQELINPFYSADDAKSDTDY
                     LTISKRYGYTSKDKIDPTTTLQAQSGQKLYAVMDGKITVTTKDLKGNETKTTNLIISD
                     NDARFIFYNISAIRVKTGDKVEAGAELGTVSGGSQKIAYAKNYGEVDSKDSQWLQDIK
                     NKDVSYGYTEQTKKNKTNVWVLVNPGFYFPFVKYAQTTVVMRTSSELSGRAKQFYDLM
                     KKFYPSATDNGIAAVVGNFAVESSITPKRAEGDYLNPPIGASDGCWDDVNWLNMGGTA
                     IYGGGYPNILHRGLGLGQWTDTTDGGTRHTMLINYANSIGKKWYELETQIRFMLEGDS
                     PYYQNILKSVLTSNSDTDSLTRTFLTKWEGINNGTLSQRQSYAKQALSWFHQSIAGGG
                     TMASSWNFPEEYRDKIKSMPTGVSMTTQPGSGYPAGQCTWYAYNRLVELGEITDLSGS
                     YGHLGDGKSWVGNLVAKGWKFSTMPTAGAVCSTAGGFDSTYPQYGHVMIVEVVNADGS
                     FLVSECNYAGNKSQIHWRVCRNAPYYTFATP"
     gene            complement(22517..24877)
                     /locus_tag="VBC23_000517"
     CDS             complement(22517..24877)
                     /locus_tag="VBC23_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6842490.1"
                     /translation="MNGTTPLTKEDKERAKQLKKALKASTQNTIKYTSLFEDGLMHIV
                     DEEYSKTWELGDTNYLTADEDEKLDIIDYYVEALNGLDSDNTYQLLIINRPVPSTLLS
                     QITYELESDDRDVYRQEYNDMITSRFATDQNNFKVEKFITVSTSARDRKQAYRKLNDV
                     ENNFSKQFQVIDIPFTPLTGTERLNIFADLLRGNPYLNVDYKDVRISGLTTKSFVAPG
                     RIWFQHHQFMIDKKYCKVLFARSFPAFLNDRLIKSITDIGIELAITIHAKPYDPADAV
                     QKINTAEAGVKMDMIKSQKVAADKGISGNLAVSGVAQATAEEAAKWSTEINDNDQKMF
                     SGIFAVMLKADTPEELADYTNRVKQAGRKHVVDFEETYYHQEEALNTILPIGKTYLDV
                     KHRFMRDMTTTNIATQIPFTNTDLQSSSPMAIYYGQNQISNNIITLDRQRDLNTSSGV
                     ILGSSGSGKSVFVKTSEVIPTILRYPDDRIIIVDPEDEYSDIGRAFDAQLIDIFPGSQ
                     THLNLMDLPDEENLSAEDADPIGEKSSLIMGLFENILQEVTDEDFSIIDRVTRLCYER
                     ITDRTPTLKDWYDILVEQPEEVAQDLALKSESYTNGSQDIFAHETNVNLNNQVVIFNL
                     KKLDGKLKPFALMVVQDYIWNQVVNNQGKLTTRIYFDEMQNQFLTDNQAEFFTNLYAR
                     VRKYGAIPTGITQNVETLLNRTEGRKLISNSEFIVLLKQKKTDLAYLTQTINLTDALI
                     RYIEKPKAKGTGLIIAGQTAVPFENPIPKNTELFRLVATDAYRQID"
     gene            complement(24852..25208)
                     /locus_tag="VBC23_000518"
     CDS             complement(24852..25208)
                     /locus_tag="VBC23_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEO6842491.1"
                     /translation="MNKLGSEFFKAIDQFERSVVGGFTLRQLIMLIGIVIGVALATLI
                     SFLKLPDILFYLVLALTVPPSFIYGIKKDEQIKEVLMFKFTVQERAYQTDFESEDMNG
                     TFIQQKGVHEWNDTFD"
     gene            complement(25315..25722)
                     /locus_tag="VBC23_000519"
     CDS             complement(25315..25722)
                     /locus_tag="VBC23_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001877537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842492.1"
                     /translation="MSEETGIAYPTLSGYNQGIRTPKKKNAQILADYFGVSVPYLLGL
                     DDNPPALNDSLEKIMVIHEVLNAGDNILVAQELLKNSLETLVKYADNDYRVQFKHHLL
                     ISNTLSEVIGKLDIVTENDELNFTPDKLNLSNN"
     gene            complement(25839..26588)
                     /locus_tag="VBC23_000520"
     CDS             complement(25839..26588)
                     /locus_tag="VBC23_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842493.1"
                     /translation="MDKIVSELTETLGSYNKTVNSSIGAINKSLMAIGYILISILFLI
                     EMLSWYRFIRNQGGEMTWKLFLEVAVKYFIAYFLVAQSGAVLNAIMWFTNGITKLIGV
                     DFKGAGFQYVPIKHGKYGIRLMIDGIGFIVGAAATLSLKVIVLLRFIELYFFKAIAPV
                     IVAFWMSDNTRHIAVNFLKMFGAVALQGAVIILLLAIWQGFRVDTLVQLDKNSWLGAY
                     ASGFSYIGKCVVFLILLIGSQRKAKQLLNVS"
     gene            complement(26647..27204)
                     /locus_tag="VBC23_000521"
     CDS             complement(26647..27204)
                     /locus_tag="VBC23_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842494.1"
                     /translation="MNKEFDYVAFAKNFEATNGRPPTADELDNAKWEHFGIRWHTPEE
                     SQAELERINASYKPTLGEHLKDALGFLGRGFFKALFFVLMSPLYLTMLFFNLIKSALG
                     VFLVWFVSKFMFLLVIGMIADLRGESEITGVSKSLVDCLFGSDFISTGNPNFFPHPVL
                     DAWIIGIVIVLLALVATFSKVEEHK"
     gene            complement(27285..27512)
                     /locus_tag="VBC23_000522"
     CDS             complement(27285..27512)
                     /locus_tag="VBC23_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842495.1"
                     /translation="MDTAVRVVTALGAILSIIGLGWVLTGAFDYFAGRKNGNPAMMDQ
                     GMTAMVSGGAVAVISAGVAAAIVAAMHSISF"
     gene            complement(27730..32253)
                     /locus_tag="VBC23_000523"
     CDS             complement(27730..32253)
                     /locus_tag="VBC23_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6842496.1"
                     /translation="MSNKFIQQSQIKKGHGFFRKKKWAKGLASGIALGAVVTFSSSAY
                     ADDIANTVDTQPEVTIVSDSNATNLADAQADNSQEHTGLTNQSGEQTGQMTDKITSDG
                     LDQSVSDAQTAGVEVDQNGTVTHSSYADAQADLAKQKEAIDQAVETQKEVDNAKATAE
                     EAAKAAGVEVSTEASKSAKTYTSVDDAKVDAKKQVDNLSTVTEAQKQVNEQLPKAVEA
                     ATGSGVKVSVKAGAKTTDAKKALENLANQVANLKKAQDTQNTISNSMAQALEEAKKAG
                     VTVSTTGSKSYDDLSKALADAQAQVAKLTEFKETQASIDEAIKKSADNAKASGVTVST
                     GDAKTYTSAADAQADSDKQTATLDKAAETQKVADKVISDLTKEAQNAGLDLTKGTSQT
                     YTSVEEAKKDIAKQERALKDATTAKANAETTNSSNKKAAADAQAAANKAINDAIAKAT
                     ASGTTVTKTSTIETTVEEAQKIAKEQSDKVNDVAAKNEQIQNENTAKKNAYDAKKAEV
                     DKKNKAAFDRAGLNYTGDYATDKATADKFNSDEIERVKSQFEKANDNNVLGATDGWVQ
                     HVGSNGYTQSGTSQFKKVQNVNGVEIVANGSLEPGKATVYVKGNVNQSHVVANMNWGN
                     VAPQGDNLTSNIGSKWAEAYDYNADGTTTQIWKGKILKWYRIPNAITMLDGSRHDAYV
                     LFHTDTSGLSSPGDEVVFWNQDGAINAVDGYRYGVNDKSDGIRTVIRVDSPDNNDNYL
                     WISLLGDFDIGQFLEADGVTVLGVGGGFATNSGVGGHRVAVDENLGLTYGKNTTPTSA
                     LTGFNSAPDGVALIAQYSNQYSTVIRNTAGGNGVAVARTDFGAQAKVSIVTPRHVNVE
                     NVETPTYTELLPVSVQDVHITPGQVTVPKAVTLHDVNVQAETHAIKLATEVAPVTVET
                     EVTTPALSATVHDITLKTAVHPVEVTQKPTNEKAVTNNDNVDINGKTVAKGSIVNWVL
                     KNGNLLAGREELTAVVMNDPFPAGFEVDSEATAKANADYYTLKTTENGYQLAGTAKLL
                     SLLNAHRDQDVEVPGFVFVGKPLNDNAHYDNAFETIYSTAKGYYKVVSNIPSIDTPDN
                     PKPTKTVTDKAGSDIDGKTVFDKDVTFHLITDYSTYVNTVVDVNTTTKAAGIYDDTDD
                     ATVKPDVTKATVTDSKGNDITAKGTFYDIEAGQPIPTEIQTFLDGANLTPNGEFIVWI
                     PNDLKDYYENYVLTGNNVTVNLPVEVIAQPGEKAENTFVQVEFGNGYQSNIVFVKVPD
                     VSPEKHAISKDGTVLDGQEVGLNQVFNYKLDGVTVPKDHDTLWQYDGKDKLDIEHDRY
                     TGNWKGVITGTEYTAKEDMTLTYDVITEDGTVIKAGDTIAAGTNYHFIFEFNQDTNDD
                     FINKIVKVTWDAQKGEWAYSIDEDFLKSLGVEGTFDADFWIEVERIKDGEVENTFINT
                     INGRELVAKVTTHTPTPETPAKPVEEVPAVKASVLPTTGEKTTALNVLASIVGLVMTV
                     GVAFGLRRKEKHN"
     gene            complement(32977..35634)
                     /locus_tag="VBC23_000524"
                     /pseudo
     CDS             complement(32977..35634)
                     /locus_tag="VBC23_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
     gene            complement(35695..36444)
                     /locus_tag="VBC23_000525"
     CDS             complement(35695..36444)
                     /locus_tag="VBC23_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6842497.1"
                     /translation="MKKVKALALFSTAILATASTGVYADEVISDGTTSPSTEQVDPTP
                     APSSSEVTPGSESNIPTPSSSSSTEAPEGSKRGSETTETEPTQPATEPEKSTETPSEQ
                     PGEVEVPTTDGGKATVVPDTSVPTNNPNITAETAQNAGASQVGTTSTVTGQVVRDVTR
                     SNPVTLYNGASLVDIRDGLVTLSTGEKVAPEVVGVKSNADGTYTAKTIQGDTVTLPHT
                     GEKNSTVLSVVGALILSVLGFGLKKRKNQEI"
     gene            complement(36455..36781)
                     /locus_tag="VBC23_000526"
     CDS             complement(36455..36781)
                     /locus_tag="VBC23_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842498.1"
                     /translation="MSNLLQVSDKPFLTLSDLRLLGRQGEAMAYLDDGRQVIIKPKFA
                     VVQKKRTINGKITIVGEDILRFHEIYSAIKFIKRKHFIIAERVKRNGKYVLVLTGRGY
                     HRQRKE"
     gene            complement(37015..37179)
                     /locus_tag="VBC23_000527"
     CDS             complement(37015..37179)
                     /locus_tag="VBC23_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842499.1"
                     /translation="MVNLILVLIAAVIVFIFVFLAVEIHDTPLMADDEMTVISSPKHD
                     HDEHHSQKAA"
     gene            complement(37172..38146)
                     /locus_tag="VBC23_000528"
     CDS             complement(37172..38146)
                     /locus_tag="VBC23_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO6842500.1"
                     /translation="MAYGRISLEQVLNSEDFYQLPKMMIKAKYYRKLRAEAKLIFALF
                     RDRITASLTNVKQGDMRFVDENGDIFIYYPIEELVQDLGWGRDKVMNLKKDLIRYGLI
                     DEVRQGVTKANRIYVKNIITDINILNMDFDEAKPFVNAVKSTEVGKFDFKKSGNPTSR
                     NREIRLQEVGKIDSSKNKESKNKESKNISSRKTEKNSNEFSQSAETYQSSSKNQSIPF
                     VEQKYYSLLQIIADKYNDQMFGFPDVLTMTHKQKMQIGQYLAEGYVTSTEVLNMIERI
                     PKDSASPLAYLLKSLENLKQERQFEQKRMAHLNAENYYSMKKEGIENG"
     gene            complement(38146..38340)
                     /locus_tag="VBC23_000529"
     CDS             complement(38146..38340)
                     /locus_tag="VBC23_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842501.1"
                     /translation="MNHSDKKRLRAKQRQSRNLVIMSIMQQTGWARNKVAISLKELED
                     YDLIKFPSRGGMMVKVGEVR"
     gene            complement(38522..>39806)
                     /locus_tag="VBC23_000530"
     CDS             complement(38522..>39806)
                     /locus_tag="VBC23_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6842502.1"
                     /translation="LMTPFKYNGMTTVAVSVISKTINQWINAVFPIHYIILLIIFISC
                     VLALCYRLFRPSFIEKNDLLKEISDITIFWLIIRLIGLALGLMTVLHIGPEMVCGKET
                     GGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEFVGVFLTPIMRPFFQLPGRSAVNCV
                     ASFVGDGTIGIALTDKQYVEGYYTSREAATISTTFSAVSITFCLVVLQNVGLTDYFGH
                     FYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPKKETSEVIPDGYNKIEWGLKLAIDK
                     SKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGTIALIISVSTPIFKILGTPFLPFLE
                     LLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSEMTRFIVATVSIVQLIYMSETGAVI
                     LGSKIPINILELFIIFIERTIISLPIIVLMAHLFF"
BASE COUNT        11904 a         7573 c         6098 g        14231 t
ORIGIN      
        1 aaaaaaatct ctcaattcat ctttaacacc atcaaattga ctacttagtt ctaattctgt
       61 ttcaattaat tcttcctttt cagaatcagt gatagcctgt atctcttcat accttgtatc
      121 ctcataatta tcttctaaaa ttgatagttc ctcttcaatt tcattgataa tttgattaag
      181 ggctgtcata acagtttctt tcggattgac atgacaaccg tgtaagtcaa ttattgcttc
      241 catatcatac ccctaaaata ccattaacgt tctaattcta gataatatgt tgaaaattct
      301 tgaatagatt gattgctttc ggcttttaaa agcatatcaa ataatcctcg ctcatcttcg
      361 tcatctacca caccactcaa catcacagca tcaagtttat aatcaatatt tttttcttta
      421 tcaatgacgt aagcagtatg aagaccacct ttagcataat ctccccatgt taaacgaaaa
      481 ataagttctt taaaactctc aaatttattt gaagataatt catagctatc aaccaatttg
      541 aatgttgcct tagtgcagta aatttcaatt ttcatttttt attctccttc aaattcttct
      601 tcaatccatt caatgatatt ttctgctaaa tcatcgataa ttattttcca tgcttcttga
      661 agtgttttaa tattttgatt atcaaaatca tcttcaattt gatctaattc gcttgtacct
      721 tttagacatt gtcttaataa gtcttgtaag tcactaaaat ttttaacgtt attatcttgc
      781 gaggaaatga caagtgttat ttcacaatcg ttagttcttt gattaaaatc agctaaatac
      841 aaatgaatta attgatatcg tcttgaaata gtttcaatca aactttgtaa gttttctaga
      901 ttcattgaac ttaccacatc aaattttacc tgtatgcttg tcatttatca cacctcgttt
      961 cactttctaa atatattata gcacttttat atataattgt aaatatattt atatatataa
     1021 ttataaatat tattgtatgt aatattgtta tactacctat gaatgccata tttttctcga
     1081 taacgtctaa acgttcgttc attaattcct gtcaattgtg caacatctct atctgacttt
     1141 ccagaaagaa acaagccaaa agcatgctgc agtctgctgt catcagctga aaacagtgcc
     1201 ctacgtccct tatatttacc ctgggctttt gcaatggcaa ttccttgtgc ctgacgctct
     1261 ttaatacgtt ctctttcagc ttgcgcctga tacttatata attccaatat caaattattt
     1321 aatagacgtc ttagattatc atcttcaatc cctctaaggg tggggagatt aagaacttcc
     1381 aaggttgcac cttttaattt tatctcatcc ataacttcgg ttaattcttt gttatttctt
     1441 cctagtcgct ccaattcagt tacaacgact atatcaccat cacgaatata atcaagcatt
     1501 gcaatcaact gaggacgctc tctacttgct ccactgacag actctttaaa aatcttttca
     1561 acgttattta attgttttaa ctgcctatct aaattttggt cagctgaact gacccttgca
     1621 taaccgattt tcaccatttt ttctcctcta ttttgcctaa ctctattgtc ctattcaaaa
     1681 atcctttaaa atcaaggtaa aacaaaaagg acaataaggt atacttattt gtcctttaca
     1741 caactaatca tcactattgt ttatttcgtc tttcataaac gtttcaaaac ttccaattgg
     1801 agcaaaatca atattaatag tattataatc atctttctta tgccacaatc gaactgcatc
     1861 tgttcctttc ttctctagcc actctcttgt aacaggttgt cgttctttta aacctagaac
     1921 atcaacaaga agccattgac caagcgcaga ttgtccataa agtttcccag tatttggatc
     1981 tttttctgtt agacttcctg attgcaagct cttcatgtca ctttgggtca ggagcgctgg
     2041 aatctttttc ccatttggca actgtaaatg gaacctaact tgtggtttat tctttttgtt
     2101 accaacataa ttcttttgac gtctttgaat ctcaaaaaca tctgaacaaa agaaatcagg
     2161 gtatttcttg tgaaattcta gaggaattgg aatatagacc tcgtttaggg gtcttaaggt
     2221 ggcactgttt ttatttttaa gtgctgcatt ccaagcatta agtccagatt ttagttctac
     2281 ttcttttgtt cgataagaat aaagaggcaa ataaacttct acaatatcat ctgaattttc
     2341 tgctttctta aaatcatcaa caaaactaaa atatgatttc agtaagaagt caaacggatc
     2401 ttcaataata ttgacatcaa attgatttaa aactaatgta tcatcagcat cagaattaaa
     2461 atattgccat atttgagagt ctccatacgt gtacttataa gttttgtgac catcactcca
     2521 cgtaaaagca gtgttagttc ttttgaattt tcctagttta tttaaatcta caagagggta
     2581 caaggtttcc tgaataacaa attttccttc atccctagta acataatgat aaatattttt
     2641 atcttttgct agacctaatc gattgtaatc aattagcatt ctttcatttt ttaattcaga
     2701 aagcttaagc gctagctcct catcactttt attgaagtaa gggtcaatat cagctttata
     2761 acgttttaat tgcatcactt tttgaaaaga tggtcgtgaa ttcatccacg tttttaagcc
     2821 gataccaatt cggtcatctc caaacactga taaaatatca tgtggtgtat ttccaatatc
     2881 aacattttca ctattaaata cttttgcgta gattgtttct tgaaattttg aatctagata
     2941 gggaataaga ttttcatgct tttgtctaaa taaattagtc aaagctccat aaactttaag
     3001 gaatttgata tattcttctc tttcctcttt agaataacgt tcccaaattg acataataat
     3061 ccaaatacct ttacttaatt ttgttcatat tatatcaaaa tgaagctagt ttatcactag
     3121 cttcattttt aaactatact ataaaattct cgttttgatt ttcgtttgat aatttttagg
     3181 tattcttcat cactcaaaat aggtaagtta tcctgataag actcaagtct tgccaaggca
     3241 tgttcatagc tatccacaaa ttctttaacg taggatgctc cttcaaattg accattgatt
     3301 ttagtataaa taatagctac ttttcctgta gattgctctg atttagctgt tgttgaatta
     3361 tttaaagcct tagctacatt agtagcaatt cgtttaataa cgggtacaac aacactgttt
     3421 cctgcctgct tataaagttg tccgtttgaa acattctctg gaactttaaa agatttagga
     3481 tatccttgaa cattaaatgt ttctttaggt gttaattttc gaatatctcc attatcagta
     3541 agaattaatg gaacattgtg tccacctgtt cccatgttgg cagtcaatgt gggaacgaca
     3601 caacttttat tttctcgaac atactgacgt ctccattgat aaacagtatc ttgttttgtc
     3661 atcacctgct caagttcact ataaaatttt tgtttacctt ctctatagta gaatatctcg
     3721 tctttatttt ctgtaaaatc aataacgtct ctcaaagaag ttgttaaatt aatttcattt
     3781 ggaaactcaa ataaatcata ggctgtttta ttgttaaaac caactacata aatacgttca
     3841 cgtttttgtg gaacatttcc ataatctttt ccgtttaaca ctttccattt tataaaataa
     3901 ttgttttcag ttaaagcttc tcggataact ttaaatgtat ttccatgatc gtgcgatact
     3961 aaatttttaa cattttcaat aaaaatagct ctaggttttc tatcttcaat catacgaagt
     4021 aactcaaaga acaaatctcc tcggtcatct tcaaaacctt tgcgataacc agcaatacta
     4081 aatgcttgac aaggaaagcc acccacaata atatcaatat tttcttgccc aatttctttt
     4141 gggtcaacag catgaatgtc tcgactgtcc aagttagtat ctggatagtt tagcttatat
     4201 gtcattctgg catacttatc aaactcattg gcatacacca ctctgaactc gttagtttgt
     4261 gtgaaaccta actcaattcc tccaacacca ctaaaaaaac cagctaggtt atatttctta
     4321 gattgttggg aacttacaga acttgtcatg tatattcctc ctatattaac aataactagt
     4381 atagtataac actaaatata aaaaattgca gccaagcaat caacttatat tttaatttta
     4441 ttgctatttt tatattttgc tttctcatgc tcttctttca tctctttagc aaagtcataa
     4501 tcttttacaa tcttatctct gtgctttttg agcgtatcac gttcaatttg aagctcttta
     4561 atttcttttt gtagctcttc tagttttgtg gttttattaa ttttaccagt ttcaagaagt
     4621 tcaagactta cttcagatgg aacaatatta ttttgataat ctgataaagc tcccatcaat
     4681 ttattaagat aagtcatctt ttcgtctaac acatctaaag tcttatctgt ttcttccaat
     4741 tgtttcaaaa actgagtttg taactcttca aattgttttg agttagtaac cttgttaatt
     4801 gccaaaaagt taaattcatc aactaatcta tctaagttgg taatattttt atttttagtc
     4861 aaaatcattt caccattttg agcagatagt tgcttgatta aagttgttcc attgatgaaa
     4921 cgattttgtt cggaatggtc agcatttaaa aagtaaaaga aatcattctt tttaacaaaa
     4981 gctgtaaatg ttccatcttc attttggtca agcattcttg ctggaataga aaccgttcct
     5041 tttcgatcta agccgaaagt aattggaaca taaatagatt gactagtaag ttgctgcact
     5101 tgccaaggtt caattgtaac ctgtaattca aaatcatctt gtttgctttc ctgttcttca
     5161 tcatattttt cttttatgac atttaaatca taaacaactt cgtttgttgc aatttgttct
     5221 ttgatttttt caagtgaaaa ctttcctttt tttgataacg tacgatcacg aacaaaacgt
     5281 tcttgtggtt gatccattaa catatattta acgtacttac cacttgtatc aatttgcaag
     5341 tttaaggctt ttgctttttg taaaaagtca ttcatatcta aagagtgttt taacaaaaag
     5401 ttcaaccgtt ctttaatctc aaaacgataa ttattttttc ttctccatgc agcatattct
     5461 gtgtgggaat ttctaagcct tggttctaaa acttttgcac cataaagctc agcatattta
     5521 tttgaaattt gaaataagtt tcgagctgta tttttctgcc atcgaaattt ttttaaagtg
     5581 acttcattcg ttgtgttaaa aataatatgg ttgtgtagat gacctttatc catatgtgtt
     5641 gcaaccacaa attgataatc accaccagtt aattctaaag cggtctttct tccaatctca
     5701 ttaacttgct caggtgttaa accatcttct ggagaaaaag actgaatgat atgatgagct
     5761 aaaactcgtt gatcattttt caaatcggat aatgtttcat tattgtacag aacgtcaaca
     5821 gattgtttta gtaaaataaa atcttcataa atcgtttgcg gatcagatat atctgttaaa
     5881 tcatgaccag ataccaaacg tttcatcact tgaccatcaa caatctcgta agaataattt
     5941 tcgtcacctt ccaaatgttc agtatctaat tttaaggttg cattatcacg tgtaatataa
     6001 tcaattgcac gctttaagtt acgacttgtt ttaatctgaa gaactttcgt cactaccatt
     6061 taatttagca cgtcctttcg ttttaaattc atcaaaaagt aatttcttca tgtcatcaat
     6121 ttcagcttgt aaagctttaa atagttcatt atcaatctca tcaaaagtgt taacatagcg
     6181 aactaattga ttgacgtttt gaccaatgcg attaacttca atccttaatt tttgcaattc
     6241 tgaataatca taagttgcta tttttccatt ttgtaacatc tctaaagcat atgcttgaaa
     6301 cgtttttttg ttagcgcgag aaaaccgttg acgaatttta gcagcttcct cttcattagc
     6361 tcttaattta aattcaactg aacgttttcg ttttttatcc gccatgaact gatttccttt
     6421 ctaaaaatct gataaaatgg agcttttttc ccagccccta gaggaggact acttttactc
     6481 tggcaagatt agcaagtggt accccactta cgaaaataaa aagcaaaaaa gcctatttgt
     6541 gggacacccc cacaaacaga cttttaactt tttacacttt ggggaaatcc ccaatcccct
     6601 atttggcact tcgtgcattt ttcaaaaatg acaatttttg ataactgcat tttgataaaa
     6661 tgcaacaata gaaaaactga caattttcaa attgtcagca ttccttctca tgtcattttt
     6721 agtttttttg acctggctcc caggagaaaa cttctgtttt tatattttga taagcatctt
     6781 ctgacagctc ttctagtgta gcgatacaaa cagaaaggtc atcgagatac tcattattat
     6841 cttctgtaga caattcttca aaatcagatt ttaacatatc caataaatca tcttttgatt
     6901 tttggataat atcaatatta actgttttaa ctcctttgag ccattcactc atatcaaaat
     6961 aatagctcat taataacatt tcagttatca tcatcatttc ttctgttatc ataacttatt
     7021 tctccttcca cttttaaagt caatcctttt ttatgcgacc tacgctgcgt tactgcttca
     7081 taggtcgatt gacttttttt gattgttctt ctttagcagg tgcggtttga acagtttcac
     7141 cttttgcttt ttgtaaactt tgataaaatg gattagctgt tttttctaat tcacgttttt
     7201 gattttcatt taatttttca tcttttgaaa ccgttagatt ttgatatttt tctaaatgct
     7261 tgtcaatacg tttcataaga gagcttgctt cttcctgaac aatttcaagt tgagctttta
     7321 gattttctaa accatctggt tcttttgacc aagaagctag ataagcaaat gaatcattac
     7381 ttgtatcaaa acctaaatga ttagatacaa cataagcaat tgactcagcc tgtagttctt
     7441 ttgaagaacg agttaatttg ccttcaaaac gttctgtcaa actcttctta ttatgtaatt
     7501 cagaatgtgc catttcatgg attagtgttg aaagagtttg ctcataagtc atcccttttt
     7561 taattgtgat ttcgtttgtt tttcggctgt aagagccatc tgatttttca agttctctaa
     7621 aacgaattgg aataccattt tctttttgag aaatatctct taatgagcga taaacatttt
     7681 gataatactc tttagtaatt tggcttggaa ttacttcacc cattttaggt aaattcaatt
     7741 ctttaccttt tacaggtgat acttgactaa tatcaaaaac aggaacaggt ttaaagtatg
     7801 taagagtttc tttttctccc gtttcagaat taatgatagg gttgccatca acatctttct
     7861 taataacagt gactggcgct tgaacataaa gggctttttc acctttgtta acatgaccat
     7921 ttcttttctt ccactcattg aaagaagcta ccatacttgc ttctggtaat tgagctttga
     7981 ttaattgaat gtttcttaca gaatagttat taaagtgtga cattccttca aggaagttct
     8041 tatagttgtc actatcaaaa taatctttaa ttccttcttg taaatgttga ttgagtgctg
     8101 ctgtattttt agctttaatc atttctgata atgttaagtc acgtttattc atttcttctt
     8161 ttcgactgtc tggtgtatct gatggattag tggcatccgt accactagct ttttcaacgc
     8221 tatctaaaat accatctcca tccgtatctg gactgtatgg attagtacct agtgcaattt
     8281 cttcatcatc tgttagacca tcaccatcag aatcttttac tttctgattt tcttcttgaa
     8341 tttttcgaag ccagacatct ttataaaaat cagaattttc atcaaagaga ttatccgctc
     8401 ttgctgcttt aacaataaca tcaaaattgt tatcatatct ttcagcgtat tcatagtatt
     8461 tatcccaagc tggatacaat tcttctggaa tattagtcct gttgtcttta ccaacttgag
     8521 caccaacatt gtaaacagca tctaaaactt tagtaaagtc ttcagcagtc aattcttttt
     8581 caactacctt aacagctttt tcaggtgcta gctcttgttc aaggaatttg taaatttctt
     8641 catttctttc caaaccatca cctacatcaa gacgtacttg tttaacgacc ttaccatctt
     8701 gaactttatt aaagtaaaac ttataatagc cttctccttc atcatttaga cttgctaaat
     8761 acaaacgata atcataatct ttaatacgtt caagtaattc ttctgttaga cctgcgcctg
     8821 taaaattagg aatttctaca ttacctgata aattagcttc attaaattca acaacccatt
     8881 cagctttttg gactgtattt gataagtctt caagttgtcc attttttaca attgataaca
     8941 ttttttcacc accatctttg ataacttctt cattaacaga taagacttta aattctccgt
     9001 ctttctcgcc aatgatacct aaaacatctt tgttatttgc cattgataat tcagattcta
     9061 gcttattagt aactttcatt aatgaagctg gaagatactc accatcaacg tttctaaaag
     9121 caatcaaaac atccttatcc tctgttcgga tagcttttgc taagtctagg cgattcatgc
     9181 gttctacttc agacaaatca tcaaaaataa agctatttga gtcaacaaca gctttaaaat
     9241 caggtaatac ttgaatttta ctaaaggtag cccctcgatt agcaaccata atattttgat
     9301 aatcaccttt accagatgct agatcatcta acgttttact tgcgctttgg tcatcatcga
     9361 tagcaaaaat accaaccagg tgtggatatt gttctgcttt aaaagttacc tttaaagctt
     9421 ctagattgtc attgttctta taaaaataag ttacattact atcagccaat tcatttttat
     9481 accaatttgc attttcttga atttgtgctg catatcgatt taacttattt aattctttct
     9541 tgttaactgg atgataacca atcttataaa ttgacttttc aggttctgaa atggtaaaat
     9601 gaagcaaagg ttgagactga gaatgagtcg gtgacgactc attcaaagag tactcttcac
     9661 gagtactagg tagaggtgct gcctcctgac tttgctcagg taaatccccg attgggttct
     9721 caaccttttt ttgttgctca aaattagctt tttctgtctc ttgaagtaat tcaggagctt
     9781 tttcttttgc tgaagataag ccatattgag ctacaattgc attaaattgt tcatcatagt
     9841 gattaataac cttatcccag gtttcatcaa ttgcagtctt taaattttta aggaacaata
     9901 ctttttgatc aaaggtccaa ttttgttccg tcaaataatc ttcaagagcg gtaggttcaa
     9961 tggaatctcc gtctagtgaa agatatcccg taactccatc cgttgttgag tctgccacat
    10021 aaagatggat attatcttga ttatattcaa tatcttttag atgataatga aaattgatga
    10081 aggcttcatc tggtgcgttt aaattttcta attcttcatc gtaccataaa tagtaagttt
    10141 caccatcagt aatatctgca atcatcccat caagtgcacg ttgacgtaat tcttctcctt
    10201 cttcttgact ttctccaaaa tcatagatac caccaccttt aagaacaaat tcaccccttt
    10261 ttggttcaac tgcttgtata gtagctaaaa acgcattaac atctttttga ctatatagat
    10321 gattagatac ttggttttct tttacccatt tttcaagatt ggctaactca gcttcaattt
    10381 caaaagcatt tccccaatct tcaccataag caagaaaacc aagtgattga ccattcttca
    10441 taacttcaag tgtccaaggt gctacatcat ctgctagatt ttctacttct tcgttgctat
    10501 gaacagctaa tgaaagacta tacccttgat ttaaataaaa ctcattcata tagacaacat
    10561 caacggtttg agttcctact aactcacttt cttgtgacaa ctgttcttga atatcatcaa
    10621 tatcccaagc tttattagcg atattttctt taggattagt ttcttgtttg agataatcat
    10681 tccaatccat ttttatttga ttagcttgac gtggtggtaa atctgatact acagggatac
    10741 catcctcttg taagccttta ataaagtttt gaccagcttc atcattatca accgctaaag
    10801 taattaaatc tggatgctct tgtaaaatgt ttgtcgtttt atttaaagca tctaaagcac
    10861 ctctaatttg ctcatttgac atggtttgag agtatttacc atcagtcaac atatcagcta
    10921 cataacggct aatgacactc tttttaagcc catccatggc tactaactta acatcttgta
    10981 gctcgtcttt atgtaattca tagtaagaca tcagatcgat tggagcttca gcaaagacta
    11041 aacgttttgg atttccaata tctaagctga aacctgcagt cccatctgaa ttcttcataa
    11101 tctgtttaag acgtccacgt tctgaatgct gcacacgatt ttcaacgatc ccctgaagac
    11161 tagcgccaat catcttgcca tcattatcac gacttttgaa tacaataaca ggttcaaaat
    11221 agtcaccttt tttgcgtgtt gctgatgcaa gattccccga tgctaaaaaa gtgttaatcg
    11281 tatcatcaga taagccacgt tcttctttca aatattgtct accaatatta aaatcagaat
    11341 gttcataacg ctctaaatag tattcaaatg gttctcttac aggtggttga acagtgacat
    11401 tctcaaagtg accagtctca agaaaagctg cagcttcctt aaaacttggc ttatgacctg
    11461 tcaattcttc tcgtaccact tgaaccaagt cgatagtatt tgagccaaca cttcttgacc
    11521 accagcagaa agagtttgta ttcggataga tatgaaaact atcgtgttct tcccaataat
    11581 aagcaccgct cgaacctggt tttaatttca tccctaagga ttcagcaaca gctacaatat
    11641 ccatactgac tagcttttgt tttcgcgctt caaagcgttg agaaagtaat tcttgcatat
    11701 ttgccataaa tttctccttt caaaccaaaa agtgccacta ttttatataa acagtagcac
    11761 ttacaattgt tttccttttt caatttgttt tgtgatagcc cacttgattt tattaacaaa
    11821 ggcttcttta tgaatttttc cctgccctat ttgtgccaat atttcttccc attttcctgt
    11881 tgtttcaggg ttagtataag caatctctag ctgatctaga taatgaataa gtaattttcc
    11941 tctttctgtc gggaaaaact gtcctgtttt cttatttttt gtgacataac ccttatcttg
    12001 aattaaatca atcatcccat cacgagttgc ggatgtacct aaattatatt ttggtaacaa
    12061 tttctttaat agaatttgtt ctgtaattcg tgttggcggc tttgtcattt cctctaagag
    12121 tttgcaatca acaacaagat ttttcccttc ctggtaatct ggtagctccc tatcttcttt
    12181 ttttaccgct aaatagttac gccatccctt atccttagtt acagcaccag ttgctttaaa
    12241 ttcaatccca cgattatcga gaataacact ggttgattga tagcgatagt caggggtaac
    12301 catcagtaag gttctgaaaa ctaccttttg ataaatgagt ttttcatcat cacttagctc
    12361 atttaaatca ggaatatttt cagttggaat aatagcatag tgttctagaa ctttcttatc
    12421 attcacataa ttttttcttg gtgagcgatt aggtgtttct aattgcctat ggattgctat
    12481 ttgataatca tctatatgtt caaccaaata agcaaattca ttggttgtaa tcaaggttga
    12541 gtctgtacgt ggataagaaa gatatccttt ttgataaaga ctttgaacca attctttggt
    12601 tttagtcgca tcaaaatggt atagcttact catttctgat tgaaaattag ttaaattata
    12661 aagcttaggt gcttcttgca ctttttcttc attcgtaacc gttttgacaa ctgctatatt
    12721 agctaactgt tgtagcatct gttctgcttc agcttttgat tgatatttag tatcattgct
    12781 aaatgtaact ttagcttcag gatcaatcag ttttagctgc caataaggac taggtttgaa
    12841 attttcaatg gcttcatcat ttcgaacgat tagactaaca ataggtgttt gaactcgtcc
    12901 aacagacata gaatgatctt tcttgtctaa atacccttct tctttcattt taatggttac
    12961 aaaaggactt agattaaaac caaccaacca atcagcatca cctcttgcat cagcttcatg
    13021 aaaatagttg atggagtctt ttggattttt aagctttgaa aaagcttgct ctaaaccttt
    13081 tttcgttaaa gagtttgccc acaagcgata tttaattttt cttagcgcac ctggtattaa
    13141 ttctaaaatt cgataagcaa tggcttctcc ttcacggtca gggtctgttc caataatgat
    13201 ggcatcaact tctttaacag cttgtttgat agccttaaat cgtttggcag tatctttttt
    13261 aggctcataa gaaaatccat ctggtaaagc tggaagatta gccatatccc aattttcaaa
    13321 gttagtatat ggattaatac gttctactaa atgaccaacg gcaggagcaa caatcacttc
    13381 agcattcaat acagatgact gaacacgcca aacaccttta tcgtttttag ggtttcctaa
    13441 agcgttcgca tactttttag cctggtcagg cttttctgct aaaagtaaat atttcaataa
    13501 aatcactctc cttttcatta tttctaccat agttataaaa tattttttat taagaaaata
    13561 aatgataaaa tagactaaaa attatttaga ggtaaccaat gctttctgat cttattttat
    13621 ttattaatat gtacacgaag tcctatcttt cttttaaaag caataaaata ctgattgatt
    13681 accaacaacg ttggataaaa ttattttcta aagtaatttc aaatccttta ccagaagaac
    13741 taaaaatacg attcaatgaa tgcttttcac ttcctattaa tgtttcagaa aatactacca
    13801 taaacattca gttcaacata ggtaatctat tagaagaaat agataaggtt aatattccaa
    13861 ctatatcaat atctacccaa cagtttattg aaaacaattc tcaaaataga ctatctctaa
    13921 tgactaattc tcaactaatt gggtatgcta ttgataaatc tttaaaaact gttagctcga
    13981 atccagtaat agtttgtaac tctgatttct ttttcaactt tgttctagat ggcaatcata
    14041 gaattgacta tgctaaaaag aaaagaattg acactatcaa ctctcaagtt atctcttcaa
    14101 aattcttagc aaccacccct catcttttcg aagataggat aagttacctt ctattttgtt
    14161 ttctagaaga tgttgcactt cttgagtctg cccttcatga aaagcaacag aaagggattt
    14221 ttggtattct aagaaccgaa tcgtctcttt taaagaagca atcaattctt ccagctgttc
    14281 agaagtatat tgacgataca tattatcaaa attaggatgt ctgacatagc caataccaat
    14341 aagattttct acatctattt ctggttttaa ctcctgttta ataggatgtt tacgtttaaa
    14401 tagtttcatt tttatcccct ttatatatta gcctcttagt acttgactga ggtaatcttc
    14461 taagactttt atcattttaa caagttcaat tatccgtgtg gtttcaacac catcaatact
    14521 aactatatac ctatcccatg ttacctcctt aattggttta taactaactg aaaaagggag
    14581 tggaagatct actatcccct ccaataaaca cttcagttcg tctttactgt taacataaaa
    14641 aggggtagaa tattttccca actcgtaagt cagctcctga taatcgttaa aactaacttc
    14701 aatatcaagg gtccctgatt ctgaaaattc tggagtgata gcagtcaacg agagactata
    14761 taataaataa tatcccccaa atttatcatg tcggtcatca attaactcta taagacctag
    14821 agatgataag ttgccttcta tcaatttttt aagtgctctc attgttaatt ctcctttata
    14881 tattttaagt aaatcacgat tttacaaaaa caaaagctac accgtttaac gatgtagctt
    14941 attttttatc tgataagttt aatagtaact gttcaagttt agtaaccaaa ggatcgtctg
    15001 gatgttcctt tagataatgt aataattcat cttctgaaat tcccagtgtt ttaaaaaatg
    15061 gactttgttt tgatgtagcc atataaacct cttggaacta ttatacttta ttacacgcta
    15121 aacttcacca taatactcat cgtcttcctc atcttctggc ggaagttcaa agatttcacc
    15181 agtcgcaaaa tccactgctt ttatttcact ttcttgtgtc tgagattggc tttctacggt
    15241 catttcaggt aattgaacgg gtacttgttc ataaacaggc tcttctgtgg cttgcgtgct
    15301 tgcttcatca gcttgtgctg cttcaatttc agttagtttt gaagtatcaa tatcattatc
    15361 tttaacaaac tgatcataac ctttcatatc tggataccag atatttttag ggtcaatttt
    15421 tccatcgcgt gcattattaa agagttctgg atcttcaacc ataaaacgag tataatcata
    15481 ccatttctca tcatttggat tattggataa ttcatccttc ataggatggt ctgttaccat
    15541 agccttttta tctcggaaaa cattttgttt tgagataaat actaatgctt catcaacacc
    15601 gatacgtgca atttcatcag gtgtcatcaa gtcacgctga tgagcttgtg tacttgtaga
    15661 tcctgataca cgttgaccac gttgttcaga atagcttttt tgtgtaatgg tttgtttacc
    15721 agcacgcatt gagaagtaac gcatagtatc ttcgtcattg gttcctaaga atagcaaggt
    15781 tgcacagtta ttaaccatgg attttcttgc tttatcacca taaatcgtat caagttgact
    15841 aatggcttga atgatgattt taacagacat ttcacgacta cgaatagatg ccaaaacttc
    15901 attgaaatga gggattttac caatgttagc aaattcatca aaaataaatt gaacgtgtac
    15961 taaatcgtca gctgtataac catctttctg accttgaata atggcatcag cattgtgtgt
    16021 taactggtca aacatgactg taaacaaagt agatgctaag aaactaaaag ctttgttggt
    16081 ttctgaaata gcgacaaaga cggctgtttt ctcagtgttc caagtatcca tttccatggt
    16141 atcagactta atcaaatctg taactgcttg gtggtcaaaa acagaatatt gtgtagcaac
    16201 catcgcaaaa acactggttc ttgtttcagc gtcaaagtta tcgttgaaca aattccattg
    16261 cttgcaagca taattaccag gcattgactc ttcaagttcc acaaacatct tttcaactgt
    16321 actaggttct ttatcatttg gacgccttat gttacgaagc aattcagcaa ccatagaaag
    16381 attaggtata taatctctag ttttgctgtc aaaatataga taaccaatca gagcacgatt
    16441 taaaagtaat tgagctttat cccaaaaagg ttctcctttg tgttcatttt cagaagcacc
    16501 ttcaaccaac gcttcggtaa tacggtcaat atctagttca gatttcatat aattgaaagg
    16561 attgaacata tctgaattag ttaaattgac caggtcaaaa actttaacct ggtaaccgtg
    16621 ttcttcaagc atcttacctg tttcacgaac taacaaccct tttggatcgg taaccacaaa
    16681 acttgagttc atttgcatga gattaggttt aacataagtg aatgttttac catcaccagg
    16741 aagaccaaca acaagaacat ttttattcaa ttgccattga aacggaagat ttttattaaa
    16801 taagcccatt tgggcattct ttgtgaaaat catattgttg gttggctctg gatctttaaa
    16861 gccagcaatc tcttgagcaa tcgcaaattt tgctgaacct gcttcttcac catgacgata
    16921 attctcatca ggttttattt ttaaataaaa catcatccca ataaagaaac caaccattgc
    16981 agctaaaata gcaaatggac tgaagttagg ataaaagtat ggtggttcaa catatttttc
    17041 cagaacaaaa gtatactggc taaataaatt tgtcatatct ggtttaggag ctatgctata
    17101 taatgcataa gctctgtgag ttagaaaagc taaaaccaga ctaatgatta gataagggat
    17161 aagaggtttc ttttgaagtt tcatcagata ctctttcctt tcttcttagt ttggacagta
    17221 tcaagttttt ctttagtgtt tttcttgatt ttgttaatct gttctttggg tgtcaattct
    17281 ttctctgatt tcatgtactt ttctttagct ttcttagggt cgttaatgat ttcattaagg
    17341 gtgtttctta aagcatccgc aataacttcc ctattaggcg cttcaaacca gacttgttta
    17401 gtgccatcac ctaaatcttt aaaagcaaag cgtacacctg attttttaag ttcatctgta
    17461 aattttttaa ggttaacatc tgcttctaaa aaatcaatat gatctttcgt caaagacgac
    17521 tggttaaaat ttttccaaga ttgttgtccc acaagttttt gttcatcata agctttatac
    17581 agttgttgcg ctgcataagc taaaaaatga aacaattgtt tacctgtttc aagtgtaaca
    17641 cgtgcacctc tatcaagaat ttgttcttgt tccataatct tctccccctt tatggataag
    17701 cagttaattc aggagctttt gagactaaat agccatattt agcttctttg ttttgtttta
    17761 ctgttaatgt taaacgtttg ttggctgttg tattatcgtc atagcgacaa ttgataacat
    17821 aggtcaaagt cgttactttt ttcgtcatca cttctttttc tagcaagaca ctgactgttg
    17881 ttgctttatc agtatcaaat tgatttaaat taagtgaatt tgaaataaaa tcttctcgag
    17941 ctgatgagtt agcaaaataa ttactaataa aatagcggca aaaaacatct gcgccctggt
    18001 taatagtagt cttttcaatt ttaggtttat ccactacttt attgttgtaa tgattagtaa
    18061 gagaaattgc taaactaaaa attgaaaata acaaagcaat cattgtccaa aagtggtgat
    18121 gactagttga agtgtgtgtt actctggcgc tagacgtggg tttagtcagc ttattctttg
    18181 ctttaatttt tttagttggc ttttccgcta ccttttgaac attcacttgc ttactaccac
    18241 gactccaaaa gaatttttta tccttcttta agccaaactc acctaataac tctttaacgt
    18301 cttgatctcc agaagatgga atagtaagct ccatcgactc tagtaattct ccatttttat
    18361 caagatgaaa aagctgaaag gttgctgttg tattgagctg taaacaacgg ttttcttccc
    18421 cttccagata aaactctaat tcttcacgat tttgaaaatc gcgctgttga ccattactta
    18481 ggtttgtaat gcgaaacatt acgcatcacc cccaagttta tccttgttat cagctaaaaa
    18541 ggcttctaag tccgatagac tcataccata ttctttaaga atttcttgtt ggcgtttagc
    18601 ttcaagttct ttaatatcag cttccaactg tttaattttc ttttgagcag cttcaaggga
    18661 tgctttcgct ttattaagtt gtttcaactt atcttttaag cgatcacttg ttgatttttg
    18721 acggcgtgct gattttgaag tgtgttgttc tgttccaaaa gtagtttcat tcattttctt
    18781 ttctcccttt tatagtgata aattatctaa gctatcatta acaattgttt tctctttaac
    18841 aggataatca ccttgatttt gattaacggc aacaatatac catttatcaa cttgaacttc
    18901 agttacttta tgagtaactt cttttttatc tttataagtt ctagtttgta gtcgtcctgt
    18961 tacctgaaga gtgtcacctt tggctgttaa cgtgttaaag ctttcagcag gtgctcccca
    19021 caagacacag tcaataaagt cagctggata agaaccatcc tctgttttat agtctctact
    19081 tgaagcaagt gtaaaagaaa taaccgaacg gtcgttgact tttcttaact ctgtattttt
    19141 agtcagacgt cctgtaataa tactttgatt taacattgcc ttctctccta ttcatcatta
    19201 ctagcaatga catctccata agaattttgt gaagctgtcg gatcttgaga atctgtaatt
    19261 aaaagcgtac tcatgttatt tacgagatat ttgccatcaa ctttattgta agttaattga
    19321 ataatagtgt tattatctac gcctgtttga gcaccttcag ttgaattctt tttctgaagt
    19381 aaatcatttg tgtaattaac atagcaaata actttatcat tggttttatc aatataaatt
    19441 tgagcatctt taaactcaaa attaacaaca aagcctttat aagcttgatt ttgtgctgtc
    19501 tcttcttcag aaacagcagc attatacatg ccatcagtca tataatcttt gtatctgtta
    19561 cgattttctt caagatcttt tttagtgtaa taagcaatca aaaactgctt aacccaaccc
    19621 tcagtcaact gatgaccttc tttaaccttt ttggttaaag ttgtatgact tgaagattga
    19681 ttatctgttt tcttgctcat tgacccaaaa cctaagccaa tgagaaagat aataataatc
    19741 cctacaatca tcatgacccg tttgaaaaaa gtcactttaa attcatctat catttttttc
    19801 ctttcatttt taaggggttg caaacgtgta gtacggagcg ttacgacaaa cacgccagtg
    19861 aatttggctt ttgttacctg catagttaca ttcagatacc aaaaaactac catcagcatt
    19921 aaccacttca acaatcataa cgtgtccata ttgtggataa gtgctatcaa agccaccagc
    19981 tgttgaacaa acagctcctg ccgttggcat cgtactaaat ttccaacctt ttgcgacaag
    20041 atttcctacc cagcttttac catcgccaag gtgaccataa cttcctgata aatccgtgat
    20101 ttcacctaat tcaaccagac gattataagc ataccaggta cattgacctg caggataacc
    20161 actaccaggt tgtgttgtca tactaacacc agttggcata ctcttaattt tatcccgata
    20221 ctcttcagga aaattccaag aggaagccat tgttccacca ccagcaattg attgatgaaa
    20281 ccaagataac gcttgtttgg cataactttg acgttgcgaa agtgttccgt tgttgatacc
    20341 ttcccatttt gttaagaagg ttctggtaag actatctgta tcagaattag aggttaacac
    20401 actttttaaa atgttttgat agtaaggtga atcaccttct agcataaatc gaatttgtgt
    20461 ttctagctca taccattttt taccaattga attagcataa ttgattaaca tggtatgacg
    20521 agtaccgcca tcggtggtat ccgtccattg accaagtcca agcccacgat gaaggatatt
    20581 aggataacca ccaccgtaga tcgctgtacc acccatattc aaccaattaa catcatccca
    20641 acagccatca gatgcaccaa taggagggtt aagataatca ccttcagctc gttttggggt
    20701 aatagatgac tcaacggcaa agttcccaac cactgcagca ataccattat cagtagcgct
    20761 tggataaaac ttcttcatca agtcataaaa ttgtttagca cgtccactca attcagatga
    20821 tgttcgcata acgacagttg tttgagcgta cttaacaaat gggaaataaa aaccaggatt
    20881 aactaacacc caaacgtttg tcttattttt ctttgtctgc tccgtatatc catatgaaac
    20941 atctttattt tttatgtctt gtaaccattg agaatcttta ctatcaactt caccatagtt
    21001 tttggcatag gcaatctttt gtgaaccacc agaaactgtt ccaagctcgg ctcctgcttc
    21061 taccttatca cctgttttaa ctcgaatagc tgatatgtta taaaaaataa aacgagcatc
    21121 gttatctgaa ataattaaat tagttgtttt agtttcgtta cccttcaaat cttttgttgt
    21181 aacggtaatt ttaccatcca tgactgcata aagcttttga cctgactgag cttgaagtgt
    21241 ggttgttgga tcgattttat cctttgaagt ataaccatat cgcttagaaa tcgtaaggta
    21301 atctgtatcg ctcttagcat catctgccga ataaaatgga ttaatcaatt cttgagtagc
    21361 taagtatttt cctgtttcgg attggctctc aagaatttct ttataatctg ctaagtcatc
    21421 tttatccaac ttcatccaag ctttaccgtt agtataaagc tcagccatag tttttaggtt
    21481 atcaatatcg ctattttctt ctttccaaat atcatccagc gcatccgaaa aatgattaaa
    21541 cccaagcgct cctgtcaatt taccacctgt cccatcatcc cagttagcat ctggttccca
    21601 ttcaccacca tagcgaaagt tcatgtaaag caagatagaa tcaatatccg tgtaatattc
    21661 aacttggtca gatgattttt ggcggtcaac ttgtgaaata tgaagccagg attgattaag
    21721 tgtaaaatca ttttgttgaa taacattgct tgaaaagatt gaggatacaa ggattaccaa
    21781 aaataaagta aaaccaacaa cagcactaac aatccacgtc atagggttag ctaatatagt
    21841 tgctgctgca cttgttgccg tttttacagc tgaagcagtg gtctgtgcta cttttgtagc
    21901 aaccttagca gtggttttag tggcaaattt agcgctatcc ttagcacttc tataagctcc
    21961 acggctaacg tttaccatac ctttgctttt tcgataagct agtttagctc ctcgccaagt
    22021 caccttacca gcctgcttag caactgcttt tgattgtctt gttcgatact gtagattttg
    22081 taaatctgac aaagtatcat cttgatccat ggcagtagat ttaatttgat gactagcatt
    22141 taatgcaatg tttttaccag tccgtctagc tttctgaccg atggttggat tagtctttct
    22201 gactttcttt ttgattttct tagtagcttt cttttcagct agcgctttct ctaagtcgtc
    22261 tacaatcttt tcaccatcaa catctttatc aatacctttc tcagctcttc ttaaagctgc
    22321 tctagcatgt ttcaccttaa tcttagcttc acgatgctct tttttagcag cattttgagc
    22381 tttcttagct gacaactgat tatgacgttg aacactttct ttaatatgac gttttctaac
    22441 cacacgcttt cttaaatcat ctttgttaga catgattcac cccctctcct tctaactatc
    22501 ctgataaata aattgattaa tcaatttgac ggtaagcatc cgttgccact aagcggaata
    22561 gttcggtatt tttaggaatt ggattttcaa atggtactgc cgtttgacca gcaatgatta
    22621 atcctgtccc ttttgctttt ggtttttcaa tataacgaat aagtgcatct gtcaaattaa
    22681 tggtttgtgt tagataagct aagtctgttt tcttttgctt caacaaaaca ataaattcac
    22741 tgtttgaaat caacttacgt ccttcagtac gattcaacaa agtttcaacg ttttgagtga
    22801 tacctgttgg aatagcacca tacttacgaa cacgggcgta aaggttagta aagaactctg
    22861 cttgattatc tgttaagaat tgattttgca tttcatcaaa gtagatacga gttgttagct
    22921 taccttgatt attaacgacc tggttccaga tataatcttg tacaaccatt aaagcaaatg
    22981 gttttaactt accatccaat tttttcagat tgaaaataac cacttgatta ttcaagttaa
    23041 cattagtttc atgagcaaaa atatcttgag acccgttcgt atatgactca gattttaatg
    23101 ctaaatcttg ggcaacttct tcaggttgtt caactaaaat gtcgtaccaa tctttcaaag
    23161 ttggtgttct atcagtaatt cgttcataac aaagacgagt cacacggtca atgattgaga
    23221 agtcttcatc agtaacttct tgcagaatat tttcaaaaag ccccatgata agtgatgact
    23281 tctcaccaat cggatcagca tcttcagctg ataaattttc ttcatctggt aaatccatca
    23341 agttcaagtg tgtctgtgaa cctgggaaaa tatcaattag ttgggcatca aatgcacgac
    23401 caatgtccga atactcatct tcagggtcta caataataat acggtcatca ggatacctca
    23461 aaatagttgg gataacttca ctggttttaa caaaaacaga cttaccagaa cctgaagaac
    23521 ctaaaatgac accagatgac gtgttcaaat cacgttgacg gtcaagcgta ataatattgt
    23581 ttgaaatttg attttgccca tagtagatag ccataggact gctactttgc aaatcagtat
    23641 tcgtaaatgg aatttgtgta gcaatgtttg ttgttgtcat atcacgcatg aagcggtgtt
    23701 taacatcaag ataagttttt ccaattggca aaatagtatt aagcgcttct tcttgatggt
    23761 agtaagtttc ttcaaaatca accacatgct tacgtccagc ttgtttgaca cggtttgtat
    23821 aatcagcaag ttcttcaggt gtatctgctt ttaacatgac tgcaaaaata cctgagaaca
    23881 ttttttggtc attatcatta atttcagtac tccatttagc ggcttcttca gcagtagctt
    23941 gagctacacc tgatactgct aaatttcctg aaataccttt gtctgcagca accttttgag
    24001 acttaatcat atccattttt acccctgcct cagctgtatt gattttctga acagcatctg
    24061 ctgggtcata cggtttagca tgaattgtaa tcgctaattc aatcccaatg tcggtaattg
    24121 acttgattag acgatcattt agaaaagctg gaaaacttct agcaaaaaga actttacagt
    24181 atttcttatc tatcataaat tgatggtgtt gaaaccaaat acgtccagga gcaacaaaag
    24241 attttgttgt taaaccacta atacgtacat ctttataatc aacattcaaa taaggattgc
    24301 cacgtaacaa atcagcaaaa atatttaatc gctctgttcc tgtcaaaggc gtaaaaggaa
    24361 tatcaatcac ttgaaattgt tttgaaaaat tgttttcaac atcattcaat ttacgataag
    24421 cttgtttacg atctcgtgca gaagttgaaa ccgtgataaa tttttcaacc ttgaaattat
    24481 tttggtcggt tgcaaaacgt gatgtaatca tatcattgta ttcttgacga taaacatcac
    24541 ggtcatcact ttcaagttca taggtaattt ggcttaaaag tgttgacgga acaggacgat
    24601 taataattag taattgataa gtattatcac tatctaagcc gtttaaagct tcaacataat
    24661 aatcaataat atcaagtttt tcatcttcat ctgccgtgag ataatttgta tcgccaagtt
    24721 cccaggtttt tgagtactcc tcatcaacaa tgtgcatcaa accgtcctca aacaaagacg
    24781 tatatttaat tgtgttttga gtactagctt tcaaagcttt tttgagctgt tttgctcgct
    24841 ctttatcttc tttagtcaaa ggtgtcgttc cattcatgga cgcccttttg ttgaataaac
    24901 gtaccattca tatcctcact ttcaaaatct gtttgataag cacgttcttg tactgtaaat
    24961 ttaaacatca aaacttcttt aatttgttcg tcttttttta taccatagat aaatgatggt
    25021 ggaaccgtca aagctaatac cagataaaac aaaatatcag gcaatttcaa gaaagagatc
    25081 aatgttgcta aagcaacacc aatgacaatc ccgattagca taatgagttg tctcaaagta
    25141 aaaccaccaa caacacttcg ttcaaattga tcaatggctt taaaaaactc agaacctaat
    25201 ttgttcattt ttctcctttc catcaaaaaa agcagttttt aaagaactgc ttttcactta
    25261 cataatccat aatctttaaa taaacttcga ttaatttttt gttatctcta acatttaatt
    25321 attagataaa ttcaatttat ctggtgtaaa attcaattca tcgttttcag taacaatatc
    25381 taattttcct atgacttctg ataaagtatt tgatatcagt aagtgatgtt tgaattgcac
    25441 cctataatcg ttatcagcat acttaactaa agtctctaag gaatttttta acaactcttg
    25501 agcaactaag atattatctc ctgcatttag cacttcatga attaccatta ttttctctaa
    25561 tgagtcattt aatgctggcg ggttatcgtc caagccaagt aaatatggaa cagatacacc
    25621 aaaataatct gcaagtatct gggcattttt ctttttcggt gttcttattc cttgattata
    25681 tcccgacaaa gttggataag caatcccagt ttcctcacta atcgttgtta atttttttga
    25741 ctttctttaa taagcttttc aatattattc ttcataattc cctctttcta aatctatttt
    25801 taggtttatc gaaaaataaa tcattcatat ttttacctct atgatacatt caacaattgt
    25861 ttagctttac gttgggaacc aatcaataaa attaagaata ctacacattt acctatatag
    25921 ctaaaacctg aagcatatgc tcctaaccaa ctatttttat ctaattgaac taatgtatca
    25981 accctaaatc cttgccaaat ggcaagcaat agaattatta cagcaccctg aagagcaact
    26041 gctccaaaca tttttaaaaa gttaacagca atatgacgtg tattatcact catccaaaaa
    26101 gcaacgatta caggtgctat tgctttaaag aaataaagtt caatgaaacg caatagaacg
    26161 attactttta aagatagagt tgctgcggca ccaacaataa aaccaatacc gtcaatcatc
    26221 aatctaattc catatttacc gtgtttaatc ggaacatatt gaaaacctgc acctttaaaa
    26281 tcaacaccaa tcagcttggt aataccatta gtaaaccaca taattgcatt taaaactgca
    26341 cctgattgag ctactaaaaa gtaggcaata aaatatttaa ccgccacttc taagaaaagc
    26401 ttccaagtca tctcaccacc ttggttccga ataaatctgt accaagagag catttcaatt
    26461 aaaaataaaa tcgaaatcag aatataacct attgccatca aagacttgtt gattgcacca
    26521 attgatgaat taactgtttt attataactt cctaaagtct cagtcaattc cgaaacaatt
    26581 ttatccatat ctctcctttc tagcataata aaaaagagcc ttgaaaataa ggttctcatc
    26641 ctataattat ttgtgttctt ctacttttga aaaagtggca actaatgcaa gtaaaacaat
    26701 gacaattcct ataatccaag catctaatac agggtgtggg aaaaaattcg gatttcctgt
    26761 agatataaaa tctgaaccaa aaagacaatc aactagactt tttgaaacgc cagtaatttc
    26821 actctcgcct cttaagtctg caatcatccc aataacaagc agaaacataa atttagaaac
    26881 aaaccaaacc aagaatacac cgagtgctga tttgattaaa ttaaagaaaa gcattgttaa
    26941 atatagtgga gacataagaa caaaaaataa agctttgaaa aatccacgtc ctagaaagcc
    27001 taaagcatcc tttaagtgtt cgcccaatgt tggcttataa gatgcgttaa tacgttccaa
    27061 ctcagcttgt gactcttctg gcgtatgcca tctaatacca aaatgttccc atttagcatt
    27121 atcaagttca tccgctgttg gtggacgtcc attagtggct tcaaagtttt ttgcaaaagc
    27181 tacataatca aattccttgt tcattttctt ttctccttgt aaccctttta cttaccttta
    27241 ttataccact ttttcaactg attatcttac atgtatgtaa gttattagaa tgaaattgag
    27301 tgcatagccg caacaattgc agctgcaaca cctgctgaaa taactgcaac agctccacct
    27361 gaaaccattg ctgtcatgcc ttggtccatc attgccggat taccattttt acgccctgca
    27421 aaataatcaa aggctcctgt taatacccag cccaaaccga tgattgataa aatagctcct
    27481 agagctgtaa ctacacgaac tgctgtgtcc ataaataaat tttctccttt aaataaaatg
    27541 tttaattaaa taaaatgttt aataaaaata aaaggctgta aatagtctta tcagttattt
    27601 ttacagcctt ttccaaccaa gtgggcaatt acctgctcct agaagagtat tatctggaga
    27661 tttagacgta acattaatta ggcaagataa tactcttcta ggaacaggca atgcctgttc
    27721 ttgttttaat tagttatgtt tttcttttct acgaagacca aacgctacac caacagtcat
    27781 aacaagacct acgatagatg ctaaaacatt taatgctgta gttttttcac cagttgttgg
    27841 aaggacacta gccttaacag ctggaacttc ttccactggt ttagcaggtg tttctggtgt
    27901 tggtgtatga gttgttactt tagcaactaa ctcacgacca ttaatagtgt tgatgaaagt
    27961 attttcaact tcaccatctt taatacgttc cacttcaatc caaaagtcag catcgaaagt
    28021 accttcaaca cctagtgatt taagaaaatc ttcatcaatg ctataagccc attcaccttt
    28081 ttgagcatcc caagtaacct taacaatttt attaatgaaa tcatcattag tatcttgatt
    28141 gaactcaaag ataaaatgat agtttgttcc agctgcgata gtatcgcctg ctttaatgac
    28201 tgtaccatct tcagtaataa catcgtatgt taacgtcata tcttctttgg cagtatactc
    28261 tgtaccagta atgacacctt tccagtttcc tgtgtagcgg tcatgttcaa tatctaactt
    28321 atctttacca tcatattgcc aaagtgtgtc atgatctttt ggaacagtca caccatccag
    28381 tttatagtta aagacttgat taagtccgac ttcttgacca tcaagaactg taccatcttt
    28441 gctaatagcg tgtttttctg gtgaaacatc tggaactttt acaaagacaa tatttgattg
    28501 ataaccatta ccaaattcaa cttgcacaaa ggtgttttca gctttttcgc ctggttgagc
    28561 aataacttca actggtaagt ttacagtaac gttattacca gttagaacat aattttcgta
    28621 gtaatctttc aaatcatttg gaatccatac aataaattca ccatttggag taaggttcgc
    28681 accatccaag aaagtttgaa tttcagttgg aattggttga cctgcttcaa tatcataaaa
    28741 tgttcctttg gcagtaatat cattgccttt actatctgtt acagtagctt ttgttacgtc
    28801 aggtttgaca gtcgcatcat cagtatcatc ataaatacct gctgcttttg ttgttgtatt
    28861 aacatcaaca actgtattaa cgtaagtact ataatctgta atcaaatgga aggtaacatc
    28921 tttatcaaat actgttttac catcaatatc actacccgct ttatctgtaa cagttttagt
    28981 tggttttgga ttatctggag tgtcaattga aggaatattt gaaacaactt tataataacc
    29041 tttagcggtt gaataaatcg tttcaaaagc attatcataa tgagcattat cattaagtgg
    29101 tttaccaaca aaaacaaaac ctggaacttc aacatcttga tcacgatgag cgttcaaaag
    29161 tgacaataat ttagctgttc ctgcaagttg ataaccattt tcggttgttt taagtgtata
    29221 atagtcagca tttgctttag cagtggcttc actatctact tcaaaacctg ctgggaatgg
    29281 atcattcatc acaacagcag tcaattcttc acgacctgct aagaggttac cattttttag
    29341 tacccagttt actattgagc ctttagcaac agttttacca ttaatatcaa cattgtcatt
    29401 gttagtaact gctttttcgt ttgttggttt ttgagttact tcaacaggat gaactgctgt
    29461 tttaagtgta atatcatgaa cagttgctga aagagctggt gtcgttactt ctgtttcaac
    29521 agtaactgga gcaacttcag tagcaagttt gatagcgtga gtttcagctt gaacattaac
    29581 atcgtgaaga gttacagctt taggtacagt tacttgtcca ggtgtaatat gtacatcttg
    29641 tactgaaact ggtaataact ctgtatatgt tggcgtttca acattttcaa cattcacatg
    29701 acgtggtgtt acgatactaa cttttgcttg tgccccaaaa tcagtacgag caacggcaac
    29761 accgttacca ccagcagtat ttcgaataac agttgaatat tgattgctat attgtgcaat
    29821 cagagcgacg ccatcgggag ctgagttaaa accagtcaat gcactagttg gagttgtatt
    29881 tttaccataa gtaagtccta agttttcatc gacagcaaca cggtgtccgc caacacctga
    29941 atttgttgca aatccaccgc caacaccaag gacagttacg ccgtctgctt ctaggaattg
    30001 accaatatca aagtctccta aaagggaaat ccagagataa ttatcgttat tatctggtga
    30061 gtcaacacga ataactgtac gaataccatc agatttatcg ttaactccgt aacgataacc
    30121 gtctacagca ttaatagcac catcttgatt ccaaaaaaca acttcatctc ctggtgatga
    30181 aagccctgaa gtatcagtat ggaacagtac gtacgcatcg tgtcgtgagc catcaagcat
    30241 agtaatagca tttggaatac gataccactt tagaatttta cccttccaaa tttgagtcgt
    30301 tgtaccatct gcgttataat cgtatgcttc agcccatttt gaaccaatgt tactagtaag
    30361 attatcacct tgtggcgcaa catttcccca gttcatattt gcaacaacat gagattgatt
    30421 aacattacct ttaacgtaaa cagttgcttt ccctggttct agagaaccgt ttgcaacaat
    30481 ttctacacca ttaacgtttt gaaccttctt gaattgactt gtacctgatt gagtgtatcc
    30541 gtttgatcca acgtgttgaa cccaaccatc agttgcacca agtacattgt tgtcattagc
    30601 tttctcaaat tgagatttaa ctcgttcgat ttcatctgaa ttaaatttat cagctgttgc
    30661 tttatctgtt gcataatcac cagtgtaatt caacccagca cgatcaaacg ctgctttatt
    30721 ctttttgtct acttcagctt ttttagcatc ataggcattt ttctttgcag tattttcatt
    30781 ttgaatttgt tcgttttttg cagcaacgtc attaacttta tcagattgct ctttagcaat
    30841 cttttgcgct tcttctacag ttgtttcaat agtgctagtt ttagtaactg ttgttccaga
    30901 agcagttgcc ttagcaatag catcattgat agctttattt gcagctgctt gagcatctgc
    30961 tgcagctttt ttgttagatg aattagtagt ttcagcattt gctttggctg ttgtagcatc
    31021 ttttagagct cgttcttgtt tagcaatatc ttttttagct tcttcaactg aagtgtatgt
    31081 ttgtgatgtt ccttttgtaa gatctaagcc agcattttga gcttctttag ttaaatctga
    31141 aataacttta tcagctactt tttgagtttc agctgcttta tctaaagttg ctgtttgttt
    31201 atcactatca gcttgagcat ctgctgctga agtgtatgtt tttgcatctc cagttgaaac
    31261 tgttacccct gaagctttag cattatcagc cgatttttta atagcttcat cgatgctagc
    31321 ttgtgtttct ttaaattcag taagtttagc gacttgagct tgagcatctg ctaaagcttt
    31381 agatagatca tcatatgatt ttgaaccagt tgtgctgact gtaacacctg cttttttagc
    31441 ttcttcaaga gcttgtgcca ttgaattgct aatggtgttt tgagtatctt gagctttttt
    31501 caaattagca acttgatttg ctaaattttc aagagctttt ttagcatcag ttgttttagc
    31561 accagcttta acactcactt ttacacctga ccccgtagct gcttccactg ctttaggaag
    31621 ttgttcattg acttgctttt gagcttcagt cacagtagaa agattatcta cttgtttttt
    31681 agcatcaacc ttggcatcat caactgaagt atacgttttt gctgatttgc ttgcttcggt
    31741 tgagacttca acacctgctg ctttagctgc ttcttcagct gttgctttag cattgtcaac
    31801 ttctttttgt gtttcaacag cttggtcaat ggcttctttt tgcttggcta aatcagcttg
    31861 agcatctgca taactagaat gagttacagt accattttga tctacttcaa ctcctgctgt
    31921 ttgagcatca gagactgatt gatctaaacc atctgatgtt attttgtcag tcatttgacc
    31981 tgtctgctca cctgattgat tggttaaacc agtgtgctct tgactgttat cagcttgagc
    32041 atctgctaaa ttagtagcat tactatccga aacaattgtt acctctggtt gagtatcaac
    32101 tgtattcgct atatcatcag catatgctga acttgaaaat gtaacgactg ctccaagagc
    32161 aatccctgaa gctagacctt tcgcccattt tttcttgcgg aaaaatccgt gtcccttttt
    32221 gatttgtgat tgttgaataa atttatttga cataataacc tcctggtttt tcctaaaaaa
    32281 ttacagcaat aaaaaacgaa cagtttattg acatgttcgg gtctacttaa aaattgaaga
    32341 aaagaataag gaaaagtagt ttatcgacct gcttaggtca ggagtaaaag acgttatgaa
    32401 taaagcaaaa actctatgat taataaccat agagttgctt tgacattatt tagctttgtt
    32461 acggcgtact tcaatcaatc cccaaagtgt cattagacaa ccaagaagga ttgtaagaat
    32521 agaactattt tcaccagttt ctggaagtac actagcttta acagctggaa cttcttctac
    32581 tgctttaact gatgatgaag aagttgaaga ttgaatttct acttgaggat cttcaactcg
    32641 ataacctaaa atcttaccag ttttatcaac aatcggaact ggagtttttc cttgagcaac
    32701 aatagcatta tattcatctt gcaatcgttg tttttcttga gcatccagaa tagtttgata
    32761 agcttttgaa gttgtatcat aaaccgcttg tgctactgct tgtttaactt gtaagtcttt
    32821 taatttaagt aattctgttt ctaacgtatc aagagcatct agcaaatgag ttcttgctgt
    32881 ttcttcttgt tttttagcat cagctaataa ttgaggggca gtttgcaaac ttgtcaaata
    32941 atgtttagct ttgtctaaat tagcttttgt agctacgaca tttgcttgag cactactaac
    33001 attatcttga gcagtagcaa gactattttg caaaccaact aaacgagctt gttcagccgt
    33061 aagatttgct tccttagtgg ctaatgtcgc ttgtttggta gaaagaactt gtttagcaga
    33121 tgctagatta gcttgtttaa ctgtaatatc agcatttaaa tcatctactg ctttttgagc
    33181 tttaacaaga ctttcctgtg cttgcttcaa tgttgattga gctgaagcaa ggttagcttt
    33241 agcagttggc gtttgaatag ctactgactg tgtttgtgtt aaggtagcac ttgctgacga
    33301 taattgtgaa attgcattat cgtatgctgc ttgagcagta gctttagctt gttgggcagt
    33361 tgattttttt gattgggctt gtgccaaagt tgattgggct gtttcaagaa ctgctttagc
    33421 tgaagttaat gcttcttgtt tagctttaat atcagcattc aaatcttcta ctgcttgagt
    33481 tgcttttgtt aaacgttctt gtgctttagt taaagctgtc tctgctaaag taagattagc
    33541 ttgagcagtc ggagtttgaa cagccactga ttggacttgc gttaaagcag aacttgctga
    33601 cgataattgt gaaattgcat tatcgtatgc tgcttgagct gttggttttg ctgattgagc
    33661 tgctgtatta gctactgttg cttgattgta actatcttta gctgcattgt aagtggcagt
    33721 tacattttca gctgattttg gattagcaat agcaattgta tcaaaattat tatttttagt
    33781 tactagttga tcttccgcaa cagaaataaa gtgtacacca gcaaaagctc cattttctga
    33841 tgaaggtttt gaaaagtcga tagcaaagta aacatgagaa ttgacatctc caccgccaac
    33901 aatatctcga gcatggcgcc attcatccgc attaaacata aagtctgtga cagcttcata
    33961 tactctttgt tttaatgtag caaatgtaat acagtcataa actgctgaag aagcccaatc
    34021 ttcataatat tgaccacctg ttgcttcatc gtcagatggt gaagttggca atccaaagta
    34081 tcgagctgca ttattgacag ctttagcatc atgaccaact tgaccttcgt ttatagcttt
    34141 tgtaacattc caaccatctg aaacatatcc atcagtaaca agatctgcaa attttacaga
    34201 tgaactagta actgaaactt gtggtgttcc gaaagctttt ctgatttgat taactaaatc
    34261 agcagcaaat tgagataatt ctttaataac atcttctgga agatcgttaa tttgatattt
    34321 tgttgaatta tcatcgttag gattactctt atagctattc aatttagcta aggcttctga
    34381 ttcttcaact aatgttttca atacttgttg acgttcttca gaagacaatg aataatcatt
    34441 atattttttc aacgcttcaa cataagcttg agtcaaagta attttgttaa tagctttata
    34501 atcattttca gctgttttat atgcagcttc agcatctttt agagcttgtt cagtttgttg
    34561 agctgttact gttttagcat ctaaatctga ttttgttgaa gtaacatttt gatttgctgt
    34621 atcaacggct gttttagcgt tatcaattgc tatttgacga tttgcatcag cttcttgtgc
    34681 ttctttcaaa cctttttgag catcagttaa cgctgtttct ttatttgctt tatcaacttt
    34741 agcagcatca gcttcatcca aaatggcttt ttgacctgta ccatctagga ttgcttgctt
    34801 gtcattgaca tctttttgtg ctgcatctac agctgattga gctgcatctt caatagcttg
    34861 tgtctgagtt accgttgctg ttttagcatc taaatctgat ttggttgacg taacagtttg
    34921 gttagcttca tcaactgttt tctgagcatt atcaatcgct tcttgacgat tggcatccgc
    34981 ttcttgcgct ttagttaaat cagattgtga ttgtttaact tgctcttgag ctgaattaac
    35041 atcattttgg gctttgtcac gatttgcaat aatttcagct tgacctgtac cgtccaagat
    35101 agcttgcttg tcgttaacat ctttttgagc tgcatctaca gctgcttggg aatcattaac
    35161 agattgttgt gcttgactaa caactgaaga ttgattgcta accgcttctt gcgctttgtt
    35221 gactacatct tctgccactt tttcagcacc ttgagcttgt gctacttctt tttccgcttc
    35281 tgtaacatca ttttgggcgg tagcaatatt ttctggtgtt gcttgatcaa cgagtttttg
    35341 agcttcactt gtttcctgtt gagctttgtc atacgctgtt tgtgctttat ctgctgattg
    35401 tgttgcatca ctcacaactt gttcttgatt gctaactgct tggttagctg tatcaagatt
    35461 agcttttgct acatcaacat cagattttgt aacattttct gttgcttgat tttgtgctac
    35521 ttccgtatta tttgatactg tcgctaaatc agtgtcatca gcatgtactt gggtaacaac
    35581 tccaaaactt gctaccactg tcgctgctgc tgtaactact tttgctttat tcataacgtc
    35641 ttttactcct ttaactgcca ttttcttatt cttccttatt ttagcacttt gaaattaaat
    35701 ttcttgattt ttgcgttttt tcaagccaaa accaagaact gataaaatga gggcacctac
    35761 aactgataaa acagttgaat ttttttcacc agtatgcggt aaagtgactg tatcaccttg
    35821 aatggttttg gctgtgtaag ttccatcagc attagattta acacctacaa cttcaggagc
    35881 tactttttca cctgttgaga gagtgactaa accgtctcga atatcaacaa gacttgcacc
    35941 gttgtacagt gtcacaggat tactacgagt cacatcacga acaacttgac cagttactgt
    36001 tgaagttgtt ccgacctgcg aagctccagc attttgagct gtttctgctg taatatttgg
    36061 attgttcgtt ggaacgcttg tatcaggaac tacggtagct ttaccaccat ctgtagttgg
    36121 gacttctacc tctccaggct gctctgatgg tgtttctgtt gacttttcag gctctgtagc
    36181 tggttgcgtt ggttctgttt ctgttgtttc agatcctcgt ttacttcctt caggagcttc
    36241 tgttgaagaa gatgatgatg gagttgggat attcgattca cttccaggtg ttacttctga
    36301 tgatgaagga gcaggtgttg gatcgacttg ctcggttgat ggagaagtgg tcccatctga
    36361 aataacttca tctgcataaa ctcctgttga tgctgtcgct aaaatagctg ttgaaaataa
    36421 agctaacgct tttacttttt tcatgtttgc tcctttattc tttcctttgt cgatgataac
    36481 ctcttcctgt taaaacgagg acatattttc cgtttcgttt aactcgttca gcgatgataa
    36541 agtgttttcg cttaatgaat ttaatagctg aataaatttc atgaaacctt aaaatatctt
    36601 caccaacaat tgtgatttta ccgttaattg ttcttttttt ctgaacaacg gcaaatttag
    36661 gtttgataat aacttgtctt ccatcgtcaa gataagccat cgcttcacct tgacgtccta
    36721 aaagacgtaa atctgataat gttaaaaatg gtttatcaga tacttgtagc aagtttgaca
    36781 taacaaactc ctttcttgct tatgtggatt tttccatctg caaaaacgca tgagtaatac
    36841 tcacacctct tcaaaaagag gtaagccaag acgtgcctgc tactttagca ggctgttaca
    36901 ccgccttcct tattcattgt tatcctatag gcttgctgat caggtcggtg tttgtgttaa
    36961 attatcaaag atctaatcaa gtagtttaaa gacttgcttg ggtcaagagt aatattaagc
    37021 agctttttgt gaatgatgct catcgtgatc gtgtttgggt gatgatataa ctgtcatctc
    37081 atcgtcagcc attaaagggg tatcatgaat ttcaactgct aagaaaacaa agataaatac
    37141 aataactgct gcaattaaaa ctaagatcaa attaaccatt ttcaatacct tcttttttca
    37201 tactgtagta attttccgcg tttaaatgag ccattctttt ttgttcaaat tgacgttctt
    37261 gcttcaaatt ctccaaagat tttagcaaat aggctaatgg tgatgcacta tccttcggaa
    37321 tacgttcaat catgtttaat acttcagtgc ttgtaacata accttctgct aaatattgac
    37381 caatttgcat tttttgcttg tgagtcattg tcaaaacatc tggaaaacca aacatttgat
    37441 cattgtattt atcagcaatt atttgtaaga gcgaataata tttttgttcg acgaatggaa
    37501 ttgattgatt ttttgatgat gactgataag tttcagcaga ctgtgaaaat tcgttagaat
    37561 ttttctcagt ctttctagaa ctaatattct tactctcttt attcttactc tctttattct
    37621 tacttgagtc gattttcccg acttcttgaa gtcggatttc ccgatttctt gaagtcggat
    37681 ttcccgattt cttgaagtcg aatttcccga cttcagttga ttttactgca tttacaaaag
    37741 gttttgcttc atcaaaatcc atatttagaa tattaatatc agtaataata tttttaacat
    37801 aaatacgatt tgcttttgta acaccttgtc gaacttcatc aattaatcca taacgaataa
    37861 gatctttttt taggttcatt actttatcac gtccccaacc taaatcttga actagttctt
    37921 caatcggata ataaatgaaa atatcgccat tttcatcaac gaaacgcata tcaccttgtt
    37981 tgacgtttgt aagcgatgca gtaattctat ctctaaaaag tgcgaatatt aatttagctt
    38041 cagctcgtaa ctttcgataa tattttgctt ttatcatcat tttaggaagt tgataaaagt
    38101 cttcactatt tagtacttgt tctaaggaaa tccgtccata tgccattacc ttacctcgcc
    38161 aacttttacc atcatgccac ctcgacttgg aaatttaatc aagtcatagt cttcaagttc
    38221 ttttaaagag atggctactt tattacgagc ccaacctgtc tgttgcatta tagacataat
    38281 aactaaattt cttgattgtc tttgtttagc acgtaatctt ttcttgtctg aatgattcat
    38341 acgttatcct ttcgtaacat ttttagcata gaaaaagaga acaattttca ttgctctctt
    38401 gatttataga tctattttta ctaatttctc aaaactctag acacttactt tcaagttcat
    38461 ttgtcttttt tcagtaaata gttctatttg ttagcaagaa atgcttctct attttctcca
    38521 actaaaagaa aagatgtgcc atcaaaacaa taataggtaa tgaaataatt gttctctcta
    38581 taaaaatgat aaataattct agaatgttga tagggatctt ggaacctaat atcaccgcac
    38641 cagtctcaga catataaatt aattgcacaa tagaaactgt tgcgacaata aaccttgtca
    38701 tctcactgtg aatttcagcc gccattatcg aaggaacaac catatctgaa aaaccaacaa
    38761 tcatggtttg actcgcaata tctgcttctg gaatccctaa aagttcaagg aacggaagga
    38821 atggtgtacc taaaatttta aaaataggtg ttgatactga aataatcaaa gctattgttc
    38881 cgacagtcat aattgttggc ataacgccaa accataaact aagtactgtc ttagaggcat
    38941 tattgacaaa agtcttcatg tttccatttg cttttgattt atcgatggca agcttaagtc
    39001 cccactctat cttgttataa ccatccggaa ttacctcaga tgtttctttc tttggtttag
    39061 tgtaataact atttgattga cgagataagg gagggatata agggataatg aatgcacaga
    39121 caacactgct aatgatgact gttagataaa aatggccaaa ataatcagtt aagccaacat
    39181 tctgcaaaac aacaagacaa aaagtgattg atactgctga aaaagtagtg gaaatcgttg
    39241 cagcttctct actggtataa taaccctcaa catactgctt atcggtcaaa gcaattccaa
    39301 tagtaccatc acctacaaat gaggctacac aattaactgc tgaccgacca ggtagctgaa
    39361 agaatggacg cataatggga gttaggaaga caccaacaaa ctccagtaga ccaaattctg
    39421 tcaaaaatgg aagaataaaa cccgcagcaa ggaaaatggt aaacaaacca ccaatcaaat
    39481 caaaaaggat aaggccgcct gtctctttcc cacataccat ttcaggacca atatgtaaaa
    39541 ccgtcatcag accaagtgcc aaaccgatca aacgaataat cagccagaaa atggtaatgt
    39601 cagaaatttc ttttagcaag tcattttttt caataaagct aggtctaaac agacgataac
    39661 aaagtgctaa cacacatgaa ataaaaatga ttagtaaaat aatataatgt ataggaaaaa
    39721 cagcgttaat ccattgatta attgtcttag agattacaga gacagctact gttgtcatac
    39781 cattatattt aaatggagtc attaat
//
LOCUS       DATRME010000010        38181 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRME010000010 DATRME010000000
VERSION     DATRME010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38181
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..117)
                     /locus_tag="VBC23_000531"
     CDS             complement(<1..117)
                     /locus_tag="VBC23_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6842503.1"
                     /translation="MAISIKSINLQNFRGFEDLHLNLNKYVTCISGHNGTGKS"
     gene            complement(242..628)
                     /locus_tag="VBC23_000532"
     CDS             complement(242..628)
                     /locus_tag="VBC23_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6842504.1"
                     /translation="MTIEEILDFHNVELAYFDNDLWPRPGIYVDEIKVVFVNKALSDE
                     SKKKVIFHELGHIDHDSNQYGRRHEEFELEANRFMIRCLLEDEFDEVEDKHEFNYLSF
                     MKRHNLKTTTDEVMVIDEYYNLLDAV"
     gene            complement(632..979)
                     /locus_tag="VBC23_000533"
     CDS             complement(632..979)
                     /locus_tag="VBC23_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842505.1"
                     /translation="MFTTFDRVKELSQKRGISLSKLEETLGLGKNSIYALKRNQPSAE
                     RLQEIADYFNVSTDYLLGRTDNPNIAKDNQEYTSDDLRKMAEKAKTFDGKPLTDSDID
                     AIQNIIEIYLKGR"
     gene            1269..1460
                     /locus_tag="VBC23_000534"
     CDS             1269..1460
                     /locus_tag="VBC23_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6842506.1"
                     /translation="MPDIANGRKKICDFLKENNIKKAALAAAYGMSRQEVTNILSGST
                     RGLKANQFILRVIEDYKID"
     gene            1494..2264
                     /locus_tag="VBC23_000535"
     CDS             1494..2264
                     /locus_tag="VBC23_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEO6842507.1"
                     /translation="MNEIFNFHGQEVRTVTINNEAYFVGKDVADILGYSNGSRDVNRH
                     VEEEDRLKYRFGTSGQDREMTIINESGLYSLILSSKLPQAKDFKRWVTSEVLPTIRKH
                     GLYAIDDLLENPDMAIAALQKLKEERQLRLQAQEEVAQKNQIIQELQPKATYYDLILQ
                     SESLVAISVIAKDYGMSAKKLNSLLHELKVQYKQGNTWLLYQKYASKGYTQSKTHPID
                     AERSKMHTYWTQKGRLFIYDLLKNKKGILPLIEQEEVA"
     gene            2342..2653
                     /locus_tag="VBC23_000536"
     CDS             2342..2653
                     /locus_tag="VBC23_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6842508.1"
                     /translation="MPKAEIIYRPANQSEKATHGDYAHLCQVWEGLTIGTAKVWAAEM
                     REHPDFRQFIDNPTHRIVFIDYEGFRLFVKWKSRNRYRPKKETLSEMIDNIKREKQLG
                     V"
     gene            2658..2804
                     /locus_tag="VBC23_000537"
     CDS             2658..2804
                     /locus_tag="VBC23_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842509.1"
                     /translation="MKKLLKKLFVKKNRKIDVRPAWKIETHGWEANARRYDNMIEHNR
                     GRTC"
     gene            2933..3344
                     /locus_tag="VBC23_000538"
                     /pseudo
     CDS             2933..3344
                     /locus_tag="VBC23_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3493..3696
                     /locus_tag="VBC23_000539"
     CDS             3493..3696
                     /locus_tag="VBC23_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842510.1"
                     /translation="MNYLEYALAYLERELEIIDNEAIEVELPDGDWEFVLNPYYEEGL
                     HDNPYYRSQVAKDILDIKGLLGR"
     gene            3786..4085
                     /locus_tag="VBC23_000540"
     CDS             3786..4085
                     /locus_tag="VBC23_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842511.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLREMNVAKVGELEEYQFAEFVEKLEAL"
     gene            4085..5242
                     /locus_tag="VBC23_000541"
     CDS             4085..5242
                     /locus_tag="VBC23_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEO6842512.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLS
                     MGALEKLIGKTTFADLLAEVIDKPQGKPVLVPEKDKRPAINSLEQAIKDFE"
     gene            5256..5819
                     /locus_tag="VBC23_000542"
     CDS             5256..5819
                     /locus_tag="VBC23_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEO6842513.1"
                     /translation="MTTTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKATIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMS
                     ISSKTKPQIIDKYKNSVDSAEEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLGGRSSADADFDEWNEEEDEDDIL"
     gene            5862..7784
                     /locus_tag="VBC23_000543"
     CDS             5862..7784
                     /locus_tag="VBC23_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEO6842514.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDTSGKNLIRYFSLPCKPSKTNGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVERELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRTNKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDFGDKIIYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGHGVVFHVHDEAIIEGSGLTIEEVNELMAQAPDWAEG
                     LPLNSEGYITKYYMKD"
     gene            7789..10173
                     /locus_tag="VBC23_000544"
     CDS             7789..10173
                     /locus_tag="VBC23_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6842515.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSGAKDNTPTNKLPSSK
                     AMNAFVCDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGFEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETIVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMGEVSGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECEPGEDFKAPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSGGKQAKNWVGFRLKSEF"
     gene            10560..10835
                     /locus_tag="VBC23_000545"
     CDS             10560..10835
                     /locus_tag="VBC23_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6842516.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTVGVPDRIVVMKTGVFFV
                     EVKAPGKKPRPSQILMHKKIKEVGQPVWVVDSYESVDIALKEMENWV"
     gene            10832..12154
                     /locus_tag="VBC23_000546"
     CDS             10832..12154
                     /locus_tag="VBC23_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6842517.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIGKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTDNVVSVKLSNMKAYKQFEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENIVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSEKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            12155..12325
                     /locus_tag="VBC23_000547"
     CDS             12155..12325
                     /locus_tag="VBC23_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842518.1"
                     /translation="MKKEYVVRIYTGREKNLEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREREND"
     gene            12607..12801
                     /locus_tag="VBC23_000548"
     CDS             12607..12801
                     /locus_tag="VBC23_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842519.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEVGIDEVIGAIKNNDIVALNGKNNA
                     VIIPKQNISHVIIHKEVTNE"
     gene            12794..13210
                     /locus_tag="VBC23_000549"
     CDS             12794..13210
                     /locus_tag="VBC23_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEO6842520.1"
                     /translation="MNKLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            13300..13752
                     /locus_tag="VBC23_000550"
     CDS             13300..13752
                     /locus_tag="VBC23_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6842521.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDITISVGEWDDD"
     gene            13742..15019
                     /locus_tag="VBC23_000551"
     CDS             13742..15019
                     /locus_tag="VBC23_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6842522.1"
                     /translation="MTIKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWIVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            15035..16567
                     /locus_tag="VBC23_000552"
     CDS             15035..16567
                     /locus_tag="VBC23_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6842523.1"
                     /translation="MEEKQFLVGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYDIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     FETKGMRLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNTYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            16527..17975
                     /locus_tag="VBC23_000553"
     CDS             16527..17975
                     /locus_tag="VBC23_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO6842524.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSVFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTIIPDEDELSIEVDPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDGVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMLDRRGLDISIIKNKDEVLAVLEKEGR"
     gene            18003..18191
                     /locus_tag="VBC23_000554"
     CDS             18003..18191
                     /locus_tag="VBC23_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842525.1"
                     /translation="MGVRPSSFFISIIHQPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            18194..18460
                     /locus_tag="VBC23_000555"
     CDS             18194..18460
                     /locus_tag="VBC23_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842526.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEALRNQNKQITELRAIVQ
                     QNAQATNEEFASVKAATLDNQSAIADIAIDVNYIKKNYKRKWGK"
     gene            18616..19191
                     /locus_tag="VBC23_000556"
     CDS             18616..19191
                     /locus_tag="VBC23_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6842527.1"
                     /translation="MAEENVTTETTEQVDTQQETVEHPKQEHERTFTRADISKMMTAE
                     RAKWEAEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLSAIAEREKAVAEREMRIET
                     QSLLAEKGLPLDFIDVVLATTADEVKTNIDNLQSIFDQAVENRVNERLTQKSPRTGNG
                     SVGMTKAEIMAIENDDERLKAIAANRNLFTR"
     gene            19210..20094
                     /locus_tag="VBC23_000557"
     CDS             19210..20094
                     /locus_tag="VBC23_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO6842528.1"
                     /translation="MAENNLNVMSDLGEIKSIDFVNKFSTNINELLKLLGVTRKQELT
                     NDLKIQTYKWSTTLDKTNPAEGETIPLSKVTRAKAKEYTVEWFKKRRATTAEAIARHG
                     VARAINEADTRIMREIQNGFKTNFFEFLKKSTTKVTGTTLQSALSQSWAKIATFNEFD
                     GSPIISFVSPLDVAKYLGDTNVGADASNVFGMTLLKNFLGMQNVIVLPSVPEGKVYTT
                     AVENLVFAYLNVNSGDLGGMFADFTDETGLIGAARTRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAPKVVPGG"
     gene            20098..20454
                     /locus_tag="VBC23_000558"
     CDS             20098..20454
                     /locus_tag="VBC23_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6842529.1"
                     /translation="MDKELLLTEVKLFKGISAKDTSQDDLIKLSINESIDRILAKLND
                     YSDTEMTEIPKRLTFIVRDVAIKRYNRLNSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            20465..20743
                     /locus_tag="VBC23_000559"
     CDS             20465..20743
                     /locus_tag="VBC23_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842530.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGRYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            20740..21084
                     /locus_tag="VBC23_000560"
     CDS             20740..21084
                     /locus_tag="VBC23_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6842531.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            21088..21447
                     /locus_tag="VBC23_000561"
     CDS             21088..21447
                     /locus_tag="VBC23_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842532.1"
                     /translation="MDYSPETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITEILI"
     gene            21459..22091
                     /locus_tag="VBC23_000562"
     CDS             21459..22091
                     /locus_tag="VBC23_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6842533.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     KLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLEGKEVYPAKFGYGKIDEIEYSEGVEKLVEASYTLNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSLLGAVPGISSEGH"
     gene            22142..22597
                     /locus_tag="VBC23_000563"
     CDS             22142..22597
                     /locus_tag="VBC23_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842534.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            22672..22902
                     /locus_tag="VBC23_000564"
     CDS             22672..22902
                     /locus_tag="VBC23_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842535.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNVSNDFAERVAKLNLHFQNL"
     gene            22921..27139
                     /locus_tag="VBC23_000565"
                     /pseudo
     CDS             22921..27139
                     /locus_tag="VBC23_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            27152..27994
                     /locus_tag="VBC23_000566"
     CDS             27152..27994
                     /locus_tag="VBC23_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6842536.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSYTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            28007..31834
                     /locus_tag="VBC23_000567"
     CDS             28007..31834
                     /locus_tag="VBC23_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6842537.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFDGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWADQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQAILDNAVFKANQALASADGKNTNYYGTEMPVDNPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKTLSDSAKKEATEALTNLISESKKLTDQMSALSKTEVEHYSTTNQQLTQIDG
                     TVKGLQTSYEALLKKDGDITQALANYKQTIDQNSASIMTNKKTADNALSSLQTQVIQT
                     SNEITTRLSQTNIEALITSKSAVIADNKVKETADSFSRELTRVNNTIDNLKIGTVNLL
                     SGTKGWTKNWFNRNNWQADDDSLSIGNFNLTVLKRKMMWSGISQSYAVKSGESYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVNSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEELTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGSFSTGGF
                     MLSKGTVELEWSESKQDIKDSVTAINTTVTQTANGWAIKNLTSSGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLADLNTGQIEFYTDSPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            31843..31995
                     /locus_tag="VBC23_000568"
     CDS             31843..31995
                     /locus_tag="VBC23_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842538.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            32006..32422
                     /locus_tag="VBC23_000569"
     CDS             32006..32422
                     /locus_tag="VBC23_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6842539.1"
                     /translation="MKQWNIVGKPFPIYQDGVVLKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIKLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            32485..32646
                     /locus_tag="VBC23_000570"
     CDS             32485..32646
                     /locus_tag="VBC23_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842540.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            32656..32946
                     /locus_tag="VBC23_000571"
     CDS             32656..32946
                     /locus_tag="VBC23_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842541.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNKEK"
     gene            32948..33199
                     /locus_tag="VBC23_000572"
     CDS             32948..33199
                     /locus_tag="VBC23_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6842542.1"
                     /translation="MINWKVRIKNKAFWSAIIPAIFLVVQAVANVFGYTLELSDLGNK
                     LLVVVNSGFSVLVIAGIVTDPTTQGVSDSSRALSYTEPK"
     gene            33329..34672
                     /locus_tag="VBC23_000573"
     CDS             33329..34672
                     /locus_tag="VBC23_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6842543.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCGFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFGEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFLGQNQGGPNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTGNTGLNKGDYFIDVS
                     AYQQADLTTTCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPSKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWIAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQEPQSQLTFNQKLDTNTKLDNSNVPYYEATLSTD
                     YYVESKPNASSTDKEFIKAGTRVRVYEKINGWSRINAPQSEQWVEDAYLIDATDM"
     gene            35623..36135
                     /locus_tag="VBC23_000574"
     CDS             35623..36135
                     /locus_tag="VBC23_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842544.1"
                     /translation="MIVDLKEIVNDYELNFCGKRCKVETNFKHLPEFMILFDIRDLYH
                     LLGIHKLKTKYRATNWVEAVKADVFLLSNYSKHPNFREVLPRVDNYNFLYEIFYQFRV
                     NVCILDKDLTKNTMKLSVVFYKDNKKKLVVVGLKRDETGVFRPATLHESRNNPYKRIR
                     HTAIKSITWI"
     gene            36363..36572
                     /locus_tag="VBC23_000575"
     CDS             36363..36572
                     /locus_tag="VBC23_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6842545.1"
                     /translation="MLYIDEFKEAIDKGYISGDTVAIVRKNGKIFDYVLPHEKVRDDE
                     VVAVESVEDVLAELGNVRCDISSCQ"
     gene            36948..37145
                     /locus_tag="VBC23_000576"
     CDS             36948..37145
                     /locus_tag="VBC23_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842546.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(37189..38121)
                     /locus_tag="VBC23_000577"
     CDS             complement(37189..38121)
                     /locus_tag="VBC23_000577"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6842547.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
BASE COUNT        13123 a         6305 c         8006 g        10747 t
ORIGIN      
        1 agattttcca gttccgttat gaccagaaat acaagttaca tatttattta aattaagatg
       61 taagtcttca aatcccctga aattttgaag atttatactt tttattgaaa tcgccatata
      121 acttttcctt tttataacct aattagctta ctttatttag aatttttata tttctaattt
      181 aataatgtaa ttgaattatt aagattcaaa acttgttcca caatcagcgc aataccattg
      241 attatactgc atccagcaga ttgtaatact cgtcgataac catgacttca tcagtcgtgg
      301 tttttaagtt gtgccttttc ataaaagata agtaattaaa ctcatgttta tcttctactt
      361 cgtcaaattc atcctcaagt aagcacctga tcataaatct attagcttca agttcaaatt
      421 cctcgtgtct gcgcccatat tggttagagt cgtggtcaat atgtccgagt tcatggaaga
      481 taactttctt ttttgattca tctgatagtg ctttatttac aaagactact tttatttcgt
      541 caacatatat ccctggacgt ggccacaaat cattgtcgaa ataagctaat tctacattat
      601 ggaagtctaa aatctcttca atagtcatag actatcttcc tttcaaataa atttcaataa
      661 tattttgtat tgcatcaata tctgaatcag taagtggctt gccatcaaat gttttagcct
      721 tttctgccat ttttctaaga tcgtcagaag tgtattcttg gttatcttta gctatattgg
      781 ggttatctgt tctacctaat aagtaatctg tagatacgtt gaagtagtct gctatttctt
      841 gtaacctttc agcagatggt tggtttcttt taagagcata gatagaattt ttgcccaatc
      901 ctagagtttc ctctaattta cttagggaaa ttcctcgctt ttgggataat tctttaactc
      961 tatcaaaagt cgtaaacatt gattttatca acctttctag ggtatgacaa aaaatattta
     1021 gagaattttg tagaaaactg ttgacaaaca gtagagaata ctctaaaata gtttttgtaa
     1081 ataagattta actagaaaaa cacctaaaca aataaatcaa aaaaaagtgt tttagcgaac
     1141 ggtattcatt gatttattag cttctttttc tatgctttca ttttagaata ttctctaaaa
     1201 tatgtcaata aaaaacgcaa aaaaatagtt gtttcttatt tacaatttta aataaaggag
     1261 gaagaaagat gcctgatatt gcaaatggac gcaaaaaaat ttgtgacttc ttaaaagaaa
     1321 acaatatcaa aaaagcagct ttagcagctg catatggcat gagtcgacaa gaagttacta
     1381 atattttaag tggctcaact cgtggcctga aagctaatca atttatctta cgagttatcg
     1441 aagattataa aattgattaa atcgcaggaa gagtttgaag aaagggataa tgtatgaacg
     1501 aaatttttaa ttttcacgga caagaagtcc gcacagtaac aatcaataat gaagcatact
     1561 ttgttggtaa agatgtggca gacattcttg gttactcaaa cgggagccga gatgtaaata
     1621 gacacgttga agaagaagat aggctaaagt accgttttgg tacctcaggt caagatagag
     1681 aaatgactat catcaatgaa tcaggtcttt actcacttat cttatctagc aagttaccgc
     1741 aagcaaaaga ttttaaacgt tgggttacgt cagaagtctt accgacaatc cgtaaacatg
     1801 gattgtacgc tattgatgat ctgctggaaa atccagacat ggcaatcgca gcacttcaga
     1861 agctcaagga agaacgtcag ctacgtttac aggcacaaga agaagtagct caaaagaatc
     1921 aaattattca ggaattacaa ccaaaagcaa cttattatga tttgatttta caaagtgaat
     1981 ccttagtggc tatctctgtc attgcaaaag attacggcat gagtgctaag aaactaaata
     2041 gtctgttgca tgaattgaaa gttcaatata agcaaggcaa tacttggcta ctatatcaga
     2101 agtatgctag caagggttac acacaatcca agacgcatcc gattgatgca gaacgcagta
     2161 agatgcacac ttactggaca caaaaaggac gcctgttcat ctatgacttg ctaaaaaaca
     2221 aaaaggggat tttgccattg attgagcagg aagaagtggc gtaataaaaa aagcacctaa
     2281 cgaagtcagg cgctcaacaa aaataactaa ctagattata acacagaaaa gagagtttaa
     2341 catgccaaaa gcagaaatta tttacagacc agctaatcag tctgaaaagg caacgcatgg
     2401 agactatgcc catctttgcc aggtttggga aggtttgaca atcggaacgg ccaaggtttg
     2461 ggcggcagaa atgcgagaac atccagactt taggcagttt attgacaatc caacgcaccg
     2521 tattgtcttt attgattacg agggtttccg tctattcgtc aaatggaaga gtcgcaatcg
     2581 gtacagaccg aaaaaagaaa cattgtctga aatgatagac aacattaaac gcgaaaaaca
     2641 attaggagta taacatcatg aagaaattat taaaaaaact atttgtaaaa aaaaatcgaa
     2701 aaatagatgt gcgtccagca tggaagattg aaacacatgg ctgggaagct aatgcacgac
     2761 gttacgacaa tatgattgaa cataatagag ggcgtacatg ttaaaagaag tattactaga
     2821 aaatgaactg ttgcgtgatg aaaatagacg actaactaat gaaatggcag attattattt
     2881 tacgaacgta gcaaaagcaa atttattgga tattatcgtc aatgaaggct acatcttaga
     2941 tagcacactg gaaaaatgta ttgctaagct agataacgtt gatagacaaa tactagaaaa
     3001 aaattggagt gatgataatg attgaagtta aaagacgaga tagcttacct atcctatctg
     3061 ctcagttgag tgttatcaag caagatttaa aattgatgct ttggattgag ctaccgacgt
     3121 taaatgacgt agagaaatat gaagctcttt atcaaggaaa tgattttaaa ttcagtgaaa
     3181 cttttttgga gcgtcaagcg atgagaaaag ccttttggag acgattagct aaggaaactt
     3241 ttaaagaaga ccaggatagg aggaactttg ttaagttggc tgagaaagaa tttaaaggtg
     3301 caactttgtc atggcaagaa tttttgtggg gtaaaaagaa atgaatattt caataaaact
     3361 cataggtgaa accctggctg gtttaaatga tattttacgc cggggggggg ggctctcttg
     3421 ctcccaaaac caagccttag cagacgcagt ttttatatta acaactttaa aacaagtaat
     3481 tgaggagaga aaatgaacta cttagaatac gcactagctt atcttgaacg tgagttagaa
     3541 attattgata acgaagctat cgaagttgag ttgccggacg gagattggga gtttgtgctt
     3601 aatccctatt acgaggaagg tttacatgat aacccatact atcgtagtca ggttgctaaa
     3661 gacattcttg atattaaagg acttttgggg aggtgaggct attgattgca tcaaaaaaga
     3721 cggcttagga aaccgtcaac aattacacac acacttaaat tataacacag aaagagagaa
     3781 acacaatgat tgaagtaact tttaaagcag agagcatggc aggtgttttc gatgctatgc
     3841 gtgaagctct ggatatgccg aagacagtaa ccaaggatgt tgttgaagaa aaaccagctc
     3901 ctaaaaagaa agaagagtct gtaactttaa cattagctga tattaagaaa atggctaaag
     3961 ccaaggttga agaagggaag tcaaaggaca tcaaggaagt cttgagagag atgaacgtcg
     4021 caaaagttgg tgaacttgaa gaataccagt ttgcggagtt tgttgagaag ttggaggctc
     4081 tctaatgcca gtagaaaatc acgcactact gtctgcttct agtgctcaca gatggcttta
     4141 ttgccctatg ctaccgagat tagaagcaga ctatcctagc cgcgacaccg tatacactca
     4201 agagggcaca agcgcccacg agctttctga aatcaaactg atgtacaagt ctggtaaaat
     4261 caccaagcgt aaatttaaca cgttaacaaa ggcttttaag gaaaactcag acttctacaa
     4321 cgaagagatg gaagagatga cggagcttta cacagatatt gttatggagc acctaaatgc
     4381 ttatgagaat gccgaaattg aacttgaaaa acgggttgat tttagcgact gggtgcctgg
     4441 cggttttggg acttcggatg tcgtcatttt ggcggatggg gtcattgaaa ttattgatct
     4501 taagtatggc aagggtatgc ctgtgtctgc taaccaaaac ccgcagatgg ggttgtacgc
     4561 tctgggagct tatgcttcct acgatatggt ttatgacttt gaccgtatca agatgaccat
     4621 tattcagcca cgtttagatt cggttagttc tgttgacatt tacgtagagg agcttctgta
     4681 ttgggcggat aatgttgtct tgcctatggc agctcaagca gatgcaggca ttggtgactg
     4741 gaacctaagt gaaaaagtat tgcagtggtc tcctgtcgca gctaaattgg tgccaagagc
     4801 gcaagaaaac tgggaattaa ttgataaata tgactatcag gagcctgtct atttatctga
     4861 tgaagccgtc gcagagatcc ttgacaaagc ctcagctatc aagaagtggg ttgagtcagt
     4921 tgaagcctat gccctgaaag aagcactctc aggaaaagaa gttccaggct ataagattgt
     4981 cgaaggtaga agtaatcgag ttatcactga taaagacaaa gcaattgata ttttacaaga
     5041 taacggcttt gacgatgaga tttttaaacc gaaagagctg ttatcaatgg gagctcttga
     5101 aaagttaata ggtaaaacca cttttgctga tttattagca gaagtaattg ataaaccaca
     5161 gggcaaacct gtacttgtcc ctgaaaagga taagcgccca gcaataaaca gtttagaaca
     5221 agcaattaaa gattttgaat aggagaaaaa taattatgac aacaacacca aacacaacta
     5281 aagtagtgac cggaaaagta cgcctaagct atgtagcttt actagagcct aaagcctttg
     5341 aaggccaaga ggctaagtat tcaacagtta tcttaattcc aaaaacagac aaagccacaa
     5401 tcaagaaaat taaagacgcg cagaaagctg cttatgaagc tgccaaggac aataaactca
     5461 aaggagttaa atgggagcgc gttaagacaa cgcttcgtga tggtgacgaa gaaatggata
     5521 ccgaagagca cccagagtac gcaggccata tgttcatgtc aatctcaagc aaaactaaac
     5581 cgcagatcat tgacaagtat aaaaactctg ttgattccgc agaagaagtc tactctggcg
     5641 tctatgctcg tgtatcactt aatgcctatg cttacaacac agcgggaaat aaaggaatct
     5701 cttgcggatt aaacaacgtc caaattgttg ctaaaggaga ctaccttggc ggccgttcgt
     5761 cagctgatgc agattttgac gagtggaacg aagaagagga cgaagacgac attttatagt
     5821 tgagggcctc tttagaggct cctcattttt aaaggagagg tatgagacat ttaaatattg
     5881 atattgaaac ctatagttcg aatgacatca aaaatggggt ttacaagtat gctgacgcag
     5941 aagattttga gattttactt ttcgcttact ctatagatgg cggagaagta gagtgccttg
     6001 atttgacaag gcagtctcta cctgaagaca tcaaagatat gttatttgat gataaagtcc
     6061 gaaagcacgc ctttaatgcc caattcgaaa gagtttgtct cagtcgttac ctcggcctac
     6121 cttactatct agatccttgc caatggcaat gcaccatggt gctggcccaa gagttgggtc
     6181 tgccttcaag cttggaaaag tgtgcgctgt atttaaaatt agcacaggaa aaagatacct
     6241 cgggtaaaaa cttaatcaga tacttttccc tgccttgcaa accaagtaaa actaacggtg
     6301 ggagaactag aaatttacca gaacacgccc ccgaaaagtg gcaaatgttt attgactact
     6361 gcatccaaga cgttgttgtt gagatggcaa ttgccgaaaa actagagtca gttcctgtgc
     6421 acgaccgtga gtgggattac tacgtctgcg accagagaat caacgacaga ggcgtggcgc
     6481 ttgataaaga gttagttgct tcggctttgt attgtaaaga tgttaagatg gaaagtttgt
     6541 ctggtgaact aaaagctcta acaggacttg ataatcctaa tagcagggcg cagctgctac
     6601 cgtggctaaa agaacacggt tattcggcta atgggctgac taaagcagat gttgaacggg
     6661 aacttaagac ggccgaagga gaacttaaga gagtcttaga acttaaacta caaaccgcga
     6721 tgtcaagtct aaaaaaatat gaagctatgg aaagagctat gtgctcagac ggacgagttc
     6781 atggactact tcagttttac ggagctagca ggacaggaag atgggcgggc agagttgtcc
     6841 aagtacagaa cttagctagg aattatataa aggatctaga tgatgctaga gagtatgtta
     6901 aaaagcgtga tattgatgct gtggagattt tatacgatag tcttaatgac actttaaagc
     6961 agctagtaag aacggcactc gtggccaaag actgctgtac cttctacgtc tctgacttct
     7021 cagcgattga ggctagggtg attgcgtggt ttgccggaga gcagtggagg cttgacgtgt
     7081 tttcgacgca cggaaaaatc tatgaggcat ccgctagcca gatgttcgga attccaattg
     7141 aggagattga taaggaacta cgccaaaaag gcaaaatctc agagttggca cttggctatc
     7201 aaggaggtcc tggagcgctc aagcagatgg gggctctaaa tatgggggtc aaggaagagg
     7261 agcttcaagg gttagttgat gactggcgca ggaccaataa gaaaatcgtc caattttgga
     7321 aagacgtaca aagagccgcc atcaaagcca tcaaatcgag agcaccgata aaacttggaa
     7381 aactacgatt tagataccgt aaaggtttcc tctttataac attgcctagc ggtaggaact
     7441 tagcttatgc aagagccaag gttgagcctg gtgactttgg agacaaaatc atctatgagg
     7501 gccaaggaga taaggcatac ttcacagcgc aagagactta tggcggtaag cttgtcgaaa
     7561 atatcgttca ggcgacggct agggatattc tagctgaagc gcttctgagg attgaagctg
     7621 caggccatgg tgttgttttc cacgttcatg atgaggctat tatcgaaggc tcaggcctga
     7681 caatcgaaga ggttaatgag ttgatggctc aagcgcctga ctgggcggag ggtcttcctt
     7741 taaatagcga aggctacata acaaagtatt atatgaagga ttagatagat gaagcaagaa
     7801 aaactaatag taaagtcttc tcctctgcaa gagctccata tcgcaacggg taattcacga
     7861 acagctaaga cgtggaaaaa tattacgcta acttggcagg agctggttga gaggttagag
     7921 aaacctacag tcacccaaga gacgtttgcg gagtaccaga agatgtctcg agcagaaaaa
     7981 gggcaagcaa aagacgtagg gggctttgtc ggtggatggc taaagcaagg taaacggaaa
     8041 aatgaaaacg ttcaaagtag gtccttggtt gcgcttgacg cagatagccc aagtaaagat
     8101 ttcttagata ggctagacct gcttgcggat tatgcctacg tactctacag tactcacagc
     8161 cactcaaaaa aagctgctaa gtaccgtctt attatcccta ctgaccgttt aatgatgcct
     8221 gatgaatatg agccagtcgc tagatacttg gctaaccaac taggcatgtc aaactttgat
     8281 gacacgactt atcaaagtgt acgcttgatg ttctggccga gtcactcaag agatgcagac
     8341 tttacgttta aatataacga cgaggctttt ctgagtgttg atgaggtgct tgacacatac
     8401 ccggactggc atgactcaag cttctggcca gaaagcccga cgcacgctgt taagagacag
     8461 cgcgaagcta aaaaacaagg tgacccgctt agtaaaaaag gacttattgg agccttttgt
     8521 cgtaactatg acattagaca ggccattgca acgttcttac ctgaggttta tgaagaagga
     8581 acaactcctg ataggtacac ctacactgag ggctcaaccg caaacggctt agttatctat
     8641 gatgatgtct tcgcttatag tcatcacggg acagatcccg tgggggatac gcttgtaaat
     8701 gcatacgacc ttgttcgtat ccataaattc ggagagcaag atagcggggc taaagataat
     8761 actcctacta ataagctacc atcaagcaaa gcgatgaatg cttttgtctg tgacttaccc
     8821 gaaattaaag actatctaat ggcggaggct ttaggcgatt tcgatgaaga gttaccagtc
     8881 gaagatgaca gaagctggct tgaaattgat gagaggggtg aacctgaggt caatagttat
     8941 ttgctagcaa cgcagattat taaggaggtt ccgatttatt gggacggctt tgagttctta
     9001 agatacgacg ctaaaaaagg catctggttg ccaaatgcag aggaatattt gaagagttat
     9061 atctcaacta agaaactcgg taaaattact aagattaggc acattagcga aaccattgta
     9121 gcgattaaag cacaggcttt ttcaagcgaa gtgtttaccg agagtgatct taacaagata
     9181 gtgctagcga acggagtcta tgacctgagg gataacagct ttaagactaa gtttgatcca
     9241 gaattgcatg ccaggtcaag ccatcccgtt gtctatgacc ccgaggcgac ctgtgaaacc
     9301 tttgagggtt ttcttaggga gaccgtcgga gctgaaaata tagatttcat ctttgagtgg
     9361 tttggctata acttttatcg tgaatacact attcaaaaaa tgctattcat ctatggcagc
     9421 ggcggtacgg gtaaatcaac actgattaat attttacgtg aaatgatagg tgctgataat
     9481 tattcagccg tgacactgca gtacctgatg caagaacgct ttgcaaaaat cggcttatat
     9541 cgtaagacag ccaactttga cactgacgca aagccccaat atttagcaga tggcgcaacc
     9601 cttaaaatgt tgacgggaga ggatactatc cacgcagacc gtaaaaataa agagccgatt
     9661 aacttttaca attatgctaa gctgtctttt gccatgaatg agctcccgcc tatgcgagat
     9721 ttcagcggag gacttaagcg ccgcatgatg atccttgaga tggataaagt tttaacgcag
     9781 gaagttaagg caaaataccc cctagataag attatgggcg aagtgtctgg tatctttaac
     9841 agagcgatgg aggggcttag aaaggcctta agtaagagag atttcagtat tagtgccagc
     9901 atgaggtcaa gtgtcgagaa atgggaaaaa ggcaacgatg tcgtagctat gttccttgaa
     9961 gacgagtgtg aacctggtga agacttcaaa gctcctgtta gggacgtcta cccagcctat
    10021 aaattctatt gtcaggattc aggctacaaa cctttggcaa aaaatgcatt taaccaccgg
    10081 ctaagagagc taaattatga aaataaaaac gttaaatctg gtggaaagca agccaaaaat
    10141 tgggtcggtt ttaggctaaa aagtgagttt taggggttac ttttttggga ttagagtttg
    10201 gtaaccctag aaaaaagtta ccaagttacc ggtttttcaa actttaaaaa taaaaaattt
    10261 aaaaataatt tttaaaaaac caaaactgaa ttggaaaaat cggtaactta gtaactcgac
    10321 cgttagaaat gtgttcgaaa agtaaccccg gtagcttttt ggtagcccta agaggctttt
    10381 cctagcaata gtttagcccc tatggttacc aatctaccac ttttcttcta ttaacttaaa
    10441 tataaatata aaaaataaat atatataaaa taaggcatat ataaaacaca tatataaaaa
    10501 agagaaagta agttgaaaaa aaacggtaac cctgaccttt attgaccaaa ggaggaagga
    10561 tgagaactga aaaagacatt gaaaattatt tgaaaaagaa aacaaaaggc ctgtgtttaa
    10621 aatttaccag tccagggacg gtaggagttc cggacagaat tgttgtcatg aaaactggtg
    10681 tattttttgt agaggtcaaa gcgcctggta aaaagccaag accgagtcaa atcctgatgc
    10741 acaaaaaaat aaaggaggta ggtcaaccag tttgggttgt tgactcctac gaatcagtgg
    10801 acatagcctt aaaagaaatg gagaactggg tgtgagactg catgagtatc aagaatacgc
    10861 taagacatgg atagtagagc acccttattg tggcctttta cttgacatgg gccttggtaa
    10921 aacgctgaca acactatcgg caatagatga gattcaaaat attttttccg aggatcataa
    10981 gattttaatc gtagctccta aaaaagtggc ggaagaaacg tggccaacgg agattgggaa
    11041 atggcatttt gatttcacct attctaaagt tttggggagt gagggaaaac gaattgaagc
    11101 cttagaaaca gaagccgata tctatttgat taatcgtgag aatgttactt ggcttgttga
    11161 atactacaag actaaatggc cgtttacctt tgttgttatt gatgagctgt caagctttaa
    11221 gtctagtaag tcaaaacggt ttagggcttt gcgaaaagtt agaccgaaag tccaacgcct
    11281 tgtagggcta acaggaaccc cagcgcctaa cagtttgatt gatttgtggc cgcagattta
    11341 tctgatggac agaggcgaca ggcttgagac gagccagact cgatttaaag acaagtattt
    11401 tgttcctgat aagcgtaatg gtccaatcat ttacagttgg gcacttaggg atggtgcaga
    11461 agcagaaatc tataacaaga ttgaggacat ctgtgtcagc atgaaagcta aagactatct
    11521 caagttaccg ccgcgaaccg acaacgttgt atcagttaag ttatctaata tgaaagccta
    11581 caaacagttt gaagctgatt tggtgttgga gtttaaaaat aaagaaatat ctgcggctaa
    11641 ttctgcggtt ttggccaata aattacttca aatggccaat ggtgctatct atgatgatga
    11701 taaagcaaca gttgctatac acgacgacaa acttgacgcg cttgagaaca tagtagaaga
    11761 aagccaaggc cagcctatct tagtttttta ccagtatcaa catgatcttg agagacttaa
    11821 gaaacgattt cctcaggctg aagagctgac gtcggttgac aagtggaatt ccgaaaaaat
    11881 accaattctt ctgtgccacc ctcaatcggc ggggcatggg cttaatctac aaaaaggcgg
    11941 gcatattatt gtttggtttg ggctaacatg gagtcttgaa tattatcagc aagctaatgc
    12001 cagattagat agacaggggc agacagaacc cgttattgtg caccacattg ttgcagaaaa
    12061 tacagttgat gaaaaagtac ttaggatttt acaaggcaaa gaaaaaaatc agaacgcctt
    12121 acttgaagca gttaaggcgc agttaggagt ctagatgaaa aaagaatatg tcgttagaat
    12181 ctacacgggt agagaaaaga atcttgaggc aaaacctcag tttgaagaga aaacctttac
    12241 gcgaaaagcg gacatgttga agttttggaa ttcttgcgaa gcaacagtta aagaaaaata
    12301 tactagggag cgggagaatg attgaagaac aataaatact tgactgctta gtcgacaaaa
    12361 tgtttcagca ggacacaaaa gcaacttaaa cgtgctagcg agcaaaatca gagacagaca
    12421 aagcggattg ctgatttgac tggaaatggg gggtaggtat gaatataatc catagaatgg
    12481 aaaacgggat aaacgctgca ggtgatttaa taacttatgc agtaccatgt tgtccagagt
    12541 gtaaaagtga aacatatagt cagaaacact gtcctttttg cggtgaaaat cttatggagg
    12601 tagcaaatga aaattgaatt ttttttaaag caaggtccta ctaagagcgt ttctaattat
    12661 ggtgaagttg gtatcgatga agtaattggt gcaataaaaa ataacgatat cgttgcgcta
    12721 aatggaaaaa acaacgcagt tattataccc aaacaaaata tttctcacgt aattattcat
    12781 aaagaggtga cgaatgaata aattatctaa cgcacagcta aaggcttttg atgaatggct
    12841 gtttgattat cgttttattg ataacaagat tgcacttcga aaacttgagc tacaaactga
    12901 tatgaataca gaagtagata aaaatatcgg tggaggaaga gcaaattttg tttcaaaagt
    12961 tactgaggat gtggttgcaa ggtgggattc agatagggag ctaaagggtc tatccaattt
    13021 caaagaagca gttattgcaa cgctaaatct tttggatgaa gagctaactg atatttttaa
    13081 tcttagatgg ggtgttggtt caagcaacac ttgggaagaa attgcctata caagacatct
    13141 gtcgcgtaag tctatttacc gcaaaagaga gcggattcta gaaatattct ccaaaaaaat
    13201 aggaatgtga cacaaaaatg tatggaattg tcatcttttt gatgataaat tgatagtatg
    13261 agtttaaagg taaggcaaat agtgtcttgc ctttttgtta tgcttggagg tgatggaaaa
    13321 ttgagtaaat taaccctaaa acagaagcgt tttgcagatg agtacatcat ctcagctaac
    13381 gcgacagcag cggctattaa agcagggtat agtaaaaaga cagcaaggtc aataggtcaa
    13441 gaaaacttga ccaaacctga cattaaagct tatatagacg agcggcttga aaaacttgaa
    13501 tctgaaaaga ttgctacgca agaagaagtc ctacaatatc tcacttcaat tatgcgagga
    13561 gaccaacaag aaaagacgct cattagcgtt ggagagtttg gccagaagat agttgacatc
    13621 gatgtaggtg caaaagatag aatcaaggca gctgagctac ttggtaagcg atatagatta
    13681 ttcaccgata aagttgaaat ggatgtgtca tcagatataa ctattagcgt aggtgagtgg
    13741 gatgacgatt aaacagcgcc ctaaaattaa tatcgtaatc aagcatccta gcaaagtttt
    13801 taacaaacat atctacgaca aactttacaa ctatagcaac ttcacggaag ttcattatgg
    13861 tggggcgtct agcggaaagt ctcatggtgt ttttcaaaag ataattttaa aagcacttaa
    13921 ccccaaattc aaacatccca gaaagatatt agttctcaga aaagttggtg caacagttag
    13981 ggactctgtc ttcgctgata ttatgtctaa tttgtcgtat tttggcgtct tggataaatg
    14041 caagataaat atgtcagctt ttagaataac attgccgaat ggctcggaat ttattttcaa
    14101 aggcatggat aaccctgaga agattaagtc aattaagggg atatctgatg tcgtcatgga
    14161 agaagctagt gagtttactc ttgacgatta cacacagctt accttacgtc ttagggacaa
    14221 gaaacattta gagaagcaag tctatctcat gttcaatcct gtgagtaaag caaactgggt
    14281 ttataaagct ttctttatca agtcacctaa gaatacagtt gtctatcaaa caacttataa
    14341 agacaaccgc ttcttagatg atgttactag agaaaatatc gaggaactag ccgataggaa
    14401 tgaagcttat tacaaaatat atgctcttgg ccagtttgct acacttgata aactaatttt
    14461 tcccaagtat gacaagcaaa tattaaacaa agacaagtta tcacacttgc cttctttttt
    14521 tggtttggac tatgggttta tcaatgaccc ttcggcattt ttgcatgtta aaatcgatga
    14581 cgcaaacaag aagttataca tcttagagga atatgtcaga aaaaatttga ccaatgataa
    14641 aatagcaaat gctataaagg accttggcta tgccaaagaa gaaatcagag gagattcggc
    14701 tgaaaagaaa tctaaccaag agctgaggaa tttaggtatt cctagaatga ttgatgttac
    14761 caaaggtcct ggaaccgtta tgcaaggaat tcagtacctg cttcagtatg attggattgt
    14821 tgatgaaagg tgtgtcaaga ctattgaaga actcgaaaat tacacttgga agaaagacaa
    14881 gaagactaat gagtatatca atgaaccagt tgacagttac aaccactgca ttgacgccat
    14941 aagatatgcc gtacaagaca gaatatatca gtcggcagat agaagtaagc gcatgaagaa
    15001 cgcgaagtac tatttttagg aatgaggtga gactttggaa gaaaaacaat ttttagtagg
    15061 aactcgtttt aatgagaatg ctaataagca atttattatg ctgcaagaag actttgaagc
    15121 aattgactat gaatcaaaac tttggataga tcaacttaag aattacattt caagatttaa
    15181 agcagaacaa ttagagcgtt tgaaagagtt aaagcgatac tatcttgggg ataataacat
    15241 caagtataga cctgctaaaa cagataaata cgcagcggat aatcgtattg caagtgattt
    15301 tgctaagtat attacggtat ttgagcaagg ttatatgctt ggcgttcccg ttgagtacaa
    15361 aaatgaaaat aaagacctcc aggcagctat tgaccttatg tcggtcagaa acaatgagga
    15421 ttaccacaac gtcaaaataa aaacagattt atcaatttat ggaagagcct acgagctgtt
    15481 aactgttgag gaaatagatg acaaaaaaac cgaagtaaaa ctctaccagc tcccagccga
    15541 gcaaactttt gttatctatg atgatacata ccagcgaaat tcgttgatgg ccgttcattt
    15601 ttacgacata gattatggct caggtaaacg caaacagatt atcaaagtat atacttccga
    15661 cacaatttat acctacgaag actataattt cgaaacaaaa ggtatgcgct taaaagagca
    15721 tgaagaacat ttttttaaag gtgtaccagt caatgaatat gctaataatg aggagcgtac
    15781 aggggcttat gagtctgtac ttgataatat tgatgcctat gatttatctc agtcagagct
    15841 tgctaatttt caacaagatt cagtcaatgc gttgcttgta atagctggta atacttacac
    15901 aggggctgat gagaatgact acttagatga cggacgatta aatcctaatg gtcgtttagc
    15961 gatctcaatt gggtttaaaa aagcccaagt gttaatttta gacgataatc ctaatccaaa
    16021 tggcgttaaa ccacaagcgt actttctcaa aaaagagtat gataccgccg gtagcgaagc
    16081 ctacaaaaac agactagttg cagacatttt gagatttact tttacgccag atacacaaga
    16141 tatgaaattt tcaggagttc aatctggtga atcaatgaag tataagctta tggcttctga
    16201 caactatcgc gagaagcaag agaggttgtt taaaaaaggc ttaatgcgac gcttacgctt
    16261 agcagctaat atctgggcca tcaaagggaa tgaagcaact acatatagcc ttgtaaatga
    16321 tacgagtata gttttcacac ctaatcttcc tcaaaacgat aatgaaattg ttacagctgc
    16381 acaaaatctc tatggcattg ttagcgatca gactatcttt gaaatcttaa atactgtcac
    16441 aggagtagac gctgaagcag agcttgaacg tttgaaagaa gaagctgata aaaagcaatc
    16501 tttaccggag cctagattgg taggtgatgc tagtggtcaa gaagaaccaa cagcagaaaa
    16561 gccttaatta ctggcaaaaa cgtcaagaag atattctaag ttatttagac agaactgatt
    16621 tagacgtctt ttcagaactg caaaagttat ataatgaaca ggcttttgag ttacaaaagg
    16681 aattgtttga tttttatact aagtattctg aagagaacaa aatgacttat caggatgtcg
    16741 ttaaaaagct acgtcatgaa gatttatcag attacgtagc taatgctaac aagtatcgta
    16801 aacaagctga gaaagaccct gagttgctaa aacgacttaa tgagcagtat gtatcagcaa
    16861 gagctacaag gatggatgca ttaaatcttg aacttgttta tcgcgcaggg atactaaaag
    16921 gtgtacttga ttcagtattt gaaaatcatt taaaaaaagc tgcctcatat gcttataaaa
    16981 aagcaatggg tggacggtca gggacaatca atggtccagt tttagaagaa ctggttagaa
    17041 ccccgtttga tggttataac tactcagaac agttgtgggg caatactgac aatcttgtca
    17101 agaatctcca aaagaaatta aaacaaggtt ttgttcgtgg cgagcatcct agagcgatag
    17161 ccagagattt agctaagcaa tttaatgttg ctaatcacag ggctgaaaca ctaatcagaa
    17221 ctgatggaac tatggtgatt aataatgcta ccgctaggcg ctacttgaat gcaggactta
    17281 agtattatcg tgatttagtt aggcttgatg acaggacaac tgagatatgt cgtaaaatcg
    17341 ctaaagaaaa caaaagaaag ctattatctg agctaaagcc tggaattaat gcagcaccct
    17401 atcatttcaa ctgcagaaca accattattc ctgatgaaga tgagctaagc attgaagtag
    17461 atccaattga tgataaaagc actaagtatt ttaaggatgt cacctcagat tggatagatg
    17521 gaaatgagca taaaccacaa ctgtcactac taaatgagta tgtaaaaaat ggcactcctt
    17581 acaaagtaga tggccataat gttgttcttg atcactcgaa ttatgaatat agagttgcta
    17641 attggttatc taaaaagaca ggattgcaag ttgacatggt gccaagagtt aattcccctg
    17701 aacatatcaa tactcctgat tatttagttg atggagttcc ttttgatcta aaagaaatca
    17761 caggttcggg taaaaacgtc attgatggca atctacgaaa agcaaaaaag caggcaacta
    17821 atatcatttt tgatataaca aaaacccctt tatcatttga agagataatg gggcagttag
    17881 aacacattta tatgttagat cgtaggggtc ttgatatatc aattatcaaa aataaagatg
    17941 aggtattagc tgtattagaa aaagaaggac gatgacccac cgcctccaca gtaaactgct
    18001 tcatgggcgt tagaccatcg tccttcttta tctcaattat acatcaacct attaaaacta
    18061 tcaaggagga aaatatgttt atttggcaaa tggtattagt ggcactaggt gtattggtgc
    18121 tcatattgat tgttggcatt gcagcaatag cggttaagtc aattattgca gaattgaaaa
    18181 aggtaggata aacatgaata agcgcattaa gaaaaaacgt aaattggaaa cagcggttgt
    18241 gatgcttgtt gcagagaatg ccatgcaagc agaagctctt agaaatcaaa acaaacaaat
    18301 tacagagctg agagcgattg tacaacaaaa cgcccaagct accaatgaag agtttgcgtc
    18361 tgttaaagct gctactttag ataaccaatc agctattgca gatattgcaa ttgacgttaa
    18421 ctatattaag aaaaattaca agcgaaaatg gggaaaataa attttaaacg aattcgaccc
    18481 ctttagaaat caaagtcgta gcgatgcggc cttttattat gtccaaactt tgttgatgac
    18541 actaaaagct acactgtttc gtcgcaggac gtaaaactag attatcggtt ggtggcgtaa
    18601 ccactaggag aaattatggc agaagaaaat gtaacaacag aaactactga acaagtcgac
    18661 actcagcaag aaactgttga acatcctaag caggaacatg agcgaacttt tactcgcgca
    18721 gatatctcaa aaatgatgac tgctgaacgt gcaaagtggg aagcagagca atcagaagca
    18781 atcgaaaaag ctcgtactga gggtgaaaga ctagctaaat tatcaaaaga tgaacgtgct
    18841 aaagaagaag aacaaaaacg tctcagtgct attgcagaac gtgaaaaagc agttgcagaa
    18901 cgtgaaatgc gcatcgaaac acaatcatta cttgctgaaa aaggattgcc actagatttt
    18961 attgatgttg ttttagctac tactgcagat gaagtgaaaa caaacattga taatttacag
    19021 tctatctttg atcaagcagt ggaaaatcgt gttaacgaaa gattaacgca aaagtcacct
    19081 cgaactggta atggctctgt cggtatgact aaagctgaaa ttatggctat tgagaatgat
    19141 gatgaacgtc tcaaagctat tgcagctaat cgtaatttat ttacaaggta agaaaggaca
    19201 tattaattta tggcagaaaa taatttaaat gtgatgtcag acttaggaga aatcaaatct
    19261 attgattttg ttaataagtt ttcaacgaat atcaatgaac tactcaagct tttgggagta
    19321 actcgcaaac aggaattgac taatgatcta aaaatccaaa cttataagtg gtctactact
    19381 ttagataaga caaatcccgc cgagggtgaa acaattcctt tatctaaagt tactcgtgca
    19441 aaagctaagg aatatactgt tgagtggttc aaaaaacgtc gtgcaactac cgctgaagca
    19501 attgcacgtc atggtgtagc tcgtgcgatt aacgaagcag atacacgcat tatgcgagaa
    19561 attcaaaatg gttttaaaac taacttcttt gaatttttaa agaagtcaac tacaaaagtg
    19621 actggaacaa ctcttcaaag tgcattatca caatcatggg caaaaattgc tacttttaat
    19681 gaattcgatg gttcacctat catctctttc gtttctccac ttgatgttgc taagtatcta
    19741 ggagatacaa atgtaggtgc agatgcttct aacgtgtttg gaatgacatt gcttaaaaac
    19801 ttcctaggta tgcaaaacgt tatcgtttta ccatcagtgc cagagggaaa agtatacaca
    19861 acagctgttg aaaaccttgt gttcgcttat ttaaacgtca atagtggcga tcttggaggg
    19921 atgtttgctg attttactga tgaaactgga ttgattggtg cggctagaac tcgtcaactt
    19981 tctaatttga cctatgaatc agtatttttt ggtgcaaatg tgctatttgc agaaattcca
    20041 gaaggcgttg tagaagcaac aattgaggct ccaaaagtag ttccaggagg ctaatagatg
    20101 gataaggagt tattactcac agaggttaag ctattcaaag ggatcagcgc caaagacact
    20161 agccaagatg atttgataaa attatccatt aatgaaagta ttgaccgtat ccttgcgaaa
    20221 ctcaatgatt actcagacac agaaatgact gaaattccta agcgtcttac ttttattgtc
    20281 agagatgtgg ctattaaaag gtataacaga cttaattcag aaggaaccgt tgaagatagc
    20341 gaagaaggaa agacttttaa gtgggacagt taccttaaag agtatgaatc aacactcaga
    20401 agcgctgcta ttgggaaggt atattcaggc aaaggggtag caagatttat ttaggaggtc
    20461 ataaatgatt tacaaagata gagttatcct agtgtatgtc gatgagcagg acgattttct
    20521 agataaaaga actgttgaaa aacctagcgg aaaaatcccc tgcatggaaa atactttcac
    20581 aaaatctgaa cagatggggt tatttggtaa atatgatttg aacgctttta agttgcactt
    20641 gcaaggccga tacgatggct ttagcaagat tatctacaag gggaaaccga ggttgataaa
    20701 gggactagcg caccataaaa atagcacggt tatttatgta tgagtcttat ttatcggatg
    20761 agaggcctag ataggttttt acgcagcgtt gagcgcaagc agaagtcagt acgaatcgct
    20821 gtagataaag agcttagtaa atcagctgct aggattgaga gacaggctaa aatactagcc
    20881 ccggttgaca ctggatggct gagagctcaa atctacagtg agcaacaacg actcttacac
    20941 tacagagtgg tttctcctgc tttatattct atttatcttg agttaggtac tcgtaaaatg
    21001 gaagcccaac catttttaga ccctgccctg agaaaagaat ggccagtgtt aatggctaat
    21061 ctcaaaaaaa tgtttaagag gtgatgcatg gattactcac cagaaacact atatttaaaa
    21121 aaggtaaaaa atagattggg agttttagac ataccaatct attttaaatt gcctaaatca
    21181 gacgttttag agccttttat tgttgtaggt acaaatatat cagacttgtc aaaaacagct
    21241 caaactggag cagttattga tgattttagc ctgaatatcg atgctttctt acctggcgat
    21301 agtcgtttgg atgcagaaga gataaaatct cgcatgctta gactgcttgg gcggaataac
    21361 caaataaagg ctcagatttt ggtagataat tcaataggac gagaagtcta tagagttgct
    21421 atcaacatta cagaaatact aatttaaagg agaattaaat ggaaactaca gttgaaatta
    21481 ctacagctaa gcctattaaa ggtaaaaata tattttattt tattcaatct gttgatgctg
    21541 aattaggtag taaagcaata ctgccagcgt atcgtacaga tggaacaacg aagctcggtg
    21601 gagattttat cgatgaacaa actcaacaag gacgtgtgct tgaaaaagca acagatgagc
    21661 actcaattga tttgacatca tactacacgc cgaaagacaa agcaattgaa attgtagaaa
    21721 tagctaaaac aactggtaaa tctgttaaag tttggcgtgt tatcgcagac gaatctttga
    21781 aagaaaaagg ttcaggaact ttagagggta aagaagttta tcccgcgaag tttggatatg
    21841 gaaaaattga cgagattgaa tattcagaag gtgttgaaaa acttgtagaa gcatcatata
    21901 cattgaatat tatcgatagt ttaaaagatg gcaaatttcc actgacggca gaagatatcg
    21961 ctttgttaac aagtgtttat gcatatcaaa acccaggcga aactactggc gattatgaca
    22021 atatccagaa aaaagaagtt tcaggaagtt tactgggagc agttccaggg atatcatcag
    22081 aaggacacta atttataagg tagcatttcg ctacctttta tttttaagga gaaatacata
    22141 aatggaatta acagtagcaa aacgcagtat tgaaattaaa tttgatttta aaacaatgtt
    22201 taagataaat aacagacttg gaactgtgaa taaagagaca ggggaacgta acgctgacgg
    22261 cgtcggtacg ctattttata aaatcttaga gcgtgatgat tcagctgtag tagatttagt
    22321 caagctgtct gtaggcagcg gtaaaaaagc attgtcagaa gatgaagtac ttgattctat
    22381 cgcagagcta gtcgaagaag aaggatcgac agaagcctta tttaaagaaa ttgagaatga
    22441 catggttgaa tctggttttt tcaaagagaa gatttcgaaa tatatcgaaa gcatggagaa
    22501 atcagtgaaa tatttagaag ctcgagaaga tgcggacgaa gctcaaatcc aaatcgtaaa
    22561 agacatgatt gggaaaatga aagacgcaat ctcttaatta actgtgccca acttggcctg
    22621 acggacattg atattattta caagtgtacg agaacagaat tagatgcaat tatggaagga
    22681 ttgcagtaca agcaaattgc agaacgtgaa aacctatctg aacttgcttt aaatctcaga
    22741 tacacgttaa atgctaaaaa cgtcaatgct aacaagctca aattagataa acaacgtaac
    22801 aaaattaaac gaacttatca aaataaacaa gatctaacta acgtttcaaa tgatttcgca
    22861 gaacgtgtgg cgaagctcaa tttacatttt caaaatttat aacaagaaag gagggatttt
    22921 atggcaggta catttgatgg gtctatattc gctgatattg gtgcaaatac taaagattat
    22981 gaggcagcta tggctagaat tgttagtact acacaaaatg cttttagaaa agcgcaggat
    23041 actgcagtta acaacagcaa caaaatgata cagatgattg gtcaaattat ggctcaatta
    23101 gcaaataatg gtcaatcatt agggcaaaga ctcggtaatg catatagtac aggtctcaag
    23161 ttatctcttg gtcaaattca aaggatagca gcatctatcg gtgaaaaaat tcccgaaccc
    23221 ataaagaatg ggttcaataa agctttttca attattcaaa atagcgtaca gaagttatcg
    23281 agcgccatcc ctcaacccat aaaatccgca ttcacaagtg caacaagcgc agtagttagc
    23341 ttttcgtcta agatcacaag tgccgtctca gctgctttca actctgttag ttctaaagcg
    23401 agtactgttg cagataaaat cagtagtagt tttgggggta gaatcacttc agcggttact
    23461 aatttagcga ctaagttaag cacaggtctt agcaatggtt ttagcaggat gtccagttct
    23521 gcaactagtg ccctaaatgg tattagtcag agattcgcaa gcgcatcatc tgctggtgaa
    23581 aaactaagaa gcactgtggg aagtatcatt tctgctttta gtttgatggc cattgctcgg
    23641 aaaggaatcc aagcaatcac aggagcaatg gacggtgcaa taagtcgtgt agataccatg
    23701 aaccgctttc cgaaaacgat ggcgctattt gggtattctg cacaacagtc taaagcatca
    23761 atagataaat tatctaaagg aattgaagga cttcctacac cactagacag tgctgttaaa
    23821 agtgcccaac aattagctat aactacaggt agcttagata aaggaacaga gctagcatta
    23881 gcatttaata atgcaatgat tggttatgga gcgactactg aaggggctga gcaagcactt
    23941 agacagttta accagtcgtt ggggtctggt aagatatatg ccgaagagtt taattctgtg
    24001 tctgaagctg cccctggctt aatgtctaaa atggcagagt cattcggatt tggtaaaaat
    24061 ggtgttcagg acttaaaagc agctctttca gacggcagta ttactgccca agaattcgct
    24121 gataaaatga ttgaactaaa caaggcgcag ggtggttttg ctgagatggc ccaatcatcg
    24181 gctggtggaa tccgaactgc atggaagaac gtacataccg ccgtcgtaaa aggcgtagca
    24241 ggtatgattt cagcctttga tgaagctgcc aaagctaatg gtatgaagac cattgcagaa
    24301 tccattaata gtattaagcc agctataaat tctgcatttg caactgtgaa ctcaataatg
    24361 ccaaaccttg ttgcagcatt cgctagaatc tccaagatgg tgaatattga ctttagtcca
    24421 ctaactagtg gaattaaatc agcttttgga ttattaaata ctgctttagg tgaatttgca
    24481 aaaactggtg aagtatcgtc agcaactatt gatagaatca aatcaaagat agcatctata
    24541 ggtcctactg caatagcagc ttgggcctta ttaaatcctg caagtgctat tgcaactatt
    24601 atgcctttaa tatcaattat tggtaagatt ggtgttgctc ttgggactgt tggttcagcg
    24661 ttgggatcat tcggagaaat ggtagccagt ggattgtcaa ctgctagtgg gctagttgga
    24721 gcttttggta gagctatgtc aggacttcct ggaatatttg gtgtagctgc aagtagaggt
    24781 cttgctgtac ttgatggaat gacaactgcg attagtagtg ctgcaatact ggcaatgtct
    24841 gctataggtc ctgcagcaat tttaggattg gtcgtagtag gtcttggttt agtaaacaat
    24901 caatttggta ctcaaattga taaatacagc aattactaaa ggacctggta ttattcaagg
    24961 ccttgtaaaa ggcattactt ctaaaatacc agctttgata gctagtggta ctcaattgat
    25021 tgctaagttt gctaatgctc taacagtatt attgccagtt attattcagg caggtgttca
    25081 attaattacc agtctggtgc aaggtattgg tcaaaacgca ggtagtttaa ttagttcggc
    25141 tcttaaagtt attggaactt ttgtaagttc aattgctagt gctctccctc aaatactagc
    25201 tgtaggtgtt caattaatac tgaacttagc tcgaggtatt gtacagaata tacctttaat
    25261 aatccagtat gctcaacaaa ttatttctaa ttttggttca agcttacaag ctaatatgcc
    25321 aagcataatt agcaacggta tagcaattag caccaatttg aatgctggta ttatccaaat
    25381 gttgccggca gttatacaaa ttgcaaccca agtcatcaca ggttttattt ccggtatagc
    25441 tcagtatttg ccacaggtaa tccaaggcgg catccagatt ataatcatgt tagtgcaagg
    25501 tattattcaa aatttaccgc aggtaatcca agcagcgatg cagattattc agtcattgat
    25561 atctggttta tcacaagcat taccacaaat tatatctgca gggatacagt tagttgtgca
    25621 gttggctgtt gcaattatta aagggttgcc gcagattatt tcagctggta ttcaattgat
    25681 tgcgggtctt ggtaaagcta tgcttgaggc tattccgaat gctctttcag gggtatggga
    25741 aggcattaaa ggtggattta gttctatgtg ggaccaaatc acaggaaaga gctcaacaag
    25801 tgccgctaag gttacatcag acatgtcaac aatgactatg aatgttggta tgcagacgac
    25861 cacaatggct aatcaaacta acatagacac aactactatg ctaaatagca ttagtcaaaa
    25921 tactggtctt gctaatttaa atgcgacaac aaatgctact cagatggcaa caaatgtaaa
    25981 tgcacaaaca gcatctatgg caaatcaagg tttgctgaat gcggcaggat ttaatgaagc
    26041 gttgagttcg aacatgactt tagcaaatac aaatgctact aatcaagcta atcaaatgac
    26101 tgctaatgtt aataacgcta ctagccaaat gagtctgggt tctctaccga atttcttgga
    26161 attatcgcaa caggcaacaa gtaatatgca aactgcacaa gcaaatgcta ccacttccgc
    26221 gaatggaatg agttctggag ttactggtgc tacagctggt atggctagtg gtgtagctaa
    26281 taacatgtta ggtataacta gcactgtatc tacagggatg aatcaagctg caaatgctgc
    26341 aaaatcaggc ggtgcaaaca tatctaatag tgtgaatagc agttttacac aagccaaatc
    26401 tacagtttca agttcaatgt caggaatgaa cagcactgtt actagtggaa tgaatagtat
    26461 taataatact gtaaaatcag gtggaaataa tattaaatcc acctttacaa gttcctttaa
    26521 ttctgctaag tctacagttt caaatgctat gtctagtatt cgttcgtcaa tttcaagtgg
    26581 aatgaacagc gctgtgagtg taacaagaag tgcaatgaat tcggttgtat ctgcaatgaa
    26641 tgcagcagca agtggtgcta gatctgcagg ttataatact gggattggat ttgcaaatgg
    26701 cctagcaagc actgcaggaa ctattatggc tattgctaat agtattgctg ctaatgtcgc
    26761 tgcaacaatc agaagcgcaa tggatattca ttcgccttcg cgagtaacgc gagaacttgg
    26821 tgcttatact ggtgaaggtt ttgcacttgg tatgcaggat tggatggatg atatcaatgc
    26881 aataggtaaa gagtacgcta atgctgtgac tgatcagcga tgggaaacag attcacaatt
    26941 agcgacatct gcaaacatat caagtgcagg tgtatcatca tcacttgata atctaagtga
    27001 cgaggtacgc aaccaacaat tgactagtcc aacgttcgag gttcataatg aacttgttgg
    27061 tgatgaaatt tatacaacta ttaaatctaa agaagcaaga gaaagtaata aagataatta
    27121 cttcaacttt gcgacataag agaggagaaa aatggattta ttagtacaaa agagtgatca
    27181 atcctttcga ttttcagaga ttggtctacg aatacttgat gtcgatgacc gttcatcatc
    27241 tcttgaggtt gatagtcgaa cagtgaaagg tcgaagcggt aaaatctttg ctagtgctaa
    27301 gtatggcaca aaaaaagtta aggttaatgg gcgcttagtt gtagcttcta ttaaagattt
    27361 tatgacaaaa aaagatgatg tgaatggctt actagtggat agtgagcctt tttacattac
    27421 taaaatgtat cctcagaaag aaaatttata tgatttcgaa ttgcctggga tgaaagcagg
    27481 tgatttagac ttattaaatc aatcttatac agcgtggcac tatcgttgga aagtttatgt
    27541 ctcaagtgaa cctgagtaca cttttgttgg taagtctagt gaaggtctca aatacaactt
    27601 tacaattgca tttgagactg cagaacttcc atttggtgaa actattgcac aaaatatagt
    27661 catcactaac ggaacgttta attattctgg tactgcacct tttagccagt tagaataccc
    27721 atggtctgta gagttaactt caactggtgg acaaagtagt ttttatcttg aaatcgatgg
    27781 gaggcgctgg gcttatcaat atagtgcacc acttaatcaa ggcgacgtta ttgtaatcac
    27841 tggtatatcc acaacattga ataagataaa tgtgactgct agaaccaatc atgaatattt
    27901 tgttttaaag caaaaagtca acaagactat tctctacagc acaaattttc aaggcacaat
    27961 caagttttta aactttaaag aactttacaa gtaaggaagt gatagattga caactttttt
    28021 agatcataaa gataatgagt ttgaagctca agctgttgtt aaaacaacta atgcagttaa
    28081 cggagaaagg tctttatcag gaacaattta tactaacgaa gaggtactaa acaaggtcga
    28141 taaaggttgg aaacttgagt ttgatgatga gtattacaag attatttatg ctaaacctac
    28201 tgatactggt aacaaaattc aagttgaatt tgatgcagtt catcaatttt tttatgattt
    28261 tgataaatca tcactacata agcaattaaa tgatggttca catactttcc aagcatattt
    28321 gaactttatt tttgatggca gcgggtatac ttacagttta gaagtagttg tcaaatcttt
    28381 cgaaaagcaa tcgtttggat ataaatcgcg acttgattta tttaatgaca tcattaagag
    28441 tgcaggagtt gaattttttg ttcgtggcaa ggttgttcga attctacaaa aaacaggaac
    28501 agatttatca acaatcgtac gtaaaaattt caatatgaat gaaatcataa ttgaaaagaa
    28561 aatcaatgat tttattacat atcagaaggg ttttggagcg tgggctgacc aagaggatca
    28621 ttccaaagga aggctcgaag tagaatatga aagtccactt gcaaaggaat atggacgaat
    28681 tgagggtgag cctgttgttg atgagcggta cactaacgct gacaatttaa aatcagtact
    28741 aaaacaaaat gtagattcgt cttacaatat cgctgtaaaa attgacattg aagatcttac
    28801 aaaagcaggt tatcagtaca ctcaacctat tgccggtgat tatattatgg ctatcaatga
    28861 gacactagga tttaaggaaa aaatccgaat agtatcgttt gaaagtgagt atgatgtaac
    28921 aggtcagctg attaaccata aagtcacttg taacgatatt ggtacaatca aaaaacaaac
    28981 tgtagctgtt agccaattat caagtaagat taataataac caagcaatac tagataatgc
    29041 agtatttaaa gcaaatcaag cactagcatc agctgatggt aaaaacacta actactatgg
    29101 tacggagatg ccagtagata atccaaaagg cacgctaaga aagggtgatt tgcttttttt
    29161 aactgttggc gatactacca gaatgtattt ctggaatggt gctgaatgga ttatcaattc
    29221 ttttagcgac gatattgatt ttgtaaagaa agaaattact aataaaatca tagaggttaa
    29281 tgcagcaatg aaacttgctg atgaacagtt aaccgaaaaa tataatgcaa ttgttgctaa
    29341 aaatgttagc caagacgaat tgatcaatca agctaaaact ttatctgact ctgcaaaaaa
    29401 agaggctaca gaagcattaa caaatcttat tagtgaatct aaaaaactga ctgaccagat
    29461 gtctgcgtta tctaaaacag aagttgagca ttattcaacg actaatcagc aactgactca
    29521 aattgatggt acagtcaagg gtttacaaac tagctatgaa gcattgttaa aaaaagatgg
    29581 agatatcact caggcacttg ctaattataa gcaaacaatt gaccaaaaca gtgcaagtat
    29641 tatgaccaac aaaaagactg ccgataatgc acttagcagt ctacagacac aagtcataca
    29701 aacgtcgaac gaaattacaa cgagattatc tcaaacaaat attgaagcgt tgattacaag
    29761 caagtctgcc gttatcgctg ataataaagt caaggaaact gcagatagtt ttagtagaga
    29821 attaactcgt gttaataata ctattgataa tttaaagata ggtacagtta atctactatc
    29881 tggtactaaa ggttggacta aaaattggtt taatcgtaac aactggcaag ctgatgatga
    29941 cagcttgtct attggtaatt tcaacttaac agtgctaaag cgtaagatga tgtggagtgg
    30001 tatatcacaa tcatatgctg taaaaagcgg agagagttat gtttttagtg catatgctaa
    30061 atcaagcata gataatgatg ttgttaattt atatttgtta ccaaatgatc aatcgcaaca
    30121 ggctaagata agtattagtt tcaagtcgat cactttaaac acggattggc aacgtatcgt
    30181 tattaaattt aacgtaaatt ctgatggcta tattttacca cgcttcgaac gctttaatga
    30241 aaatggttgg ttgtatattg caggttacca gcttgaacat ggaaatgtta caagtgacta
    30301 ttcagagtcc ccagcggatc aagaagataa aatgactgtt gaatttaata aaatcaacga
    30361 tacagttaat agtcatagtc aactgattgg taaacaaaac gagagtctga cagctacaat
    30421 acaaaaagtt gataatatac aatcaacagt atctaatgtg gatggacgct tgtcacgagt
    30481 tacccaaacg gctgacggta tagttgcaac agttagtcag cttgataaca acattttacc
    30541 tggcacaaaa aaattcacag gatggcaaac agaaggtgca gtactagagg aactaacaca
    30601 atcgccttac ccatttgttt ttaaaaaatg ggtaagcggt aataaggtgt cacctttaat
    30661 cgaatatgat gtcaaaaaag atcaagagta tacgtttact gcatacattt ctagagaaca
    30721 agcaggaaat ctatatttct acttgtatga tctttggaat catcatatta caagcacaac
    30781 tcctcgtgaa actattatta aagacgtcac tcaaaccatt agacgattta aaataacatt
    30841 tattccaact cgagacggta aaattagacc acgctttgcc atgcttgcta gtgatccagg
    30901 tagctttagt accggtggtt ttatgttatc taaaggtaca gttgagttgg aatggtctga
    30961 atctaagcaa gatattaaag atagtgtgac tgctattaat acaacagtta cacaaacggc
    31021 taacggttgg gctattaaaa atttaacttc tagcggtact gtgttgagtc agcttaattt
    31081 gacggacggt aatgtaaaac tggaaggtaa gttaatacac ttgtcaggac agtctattat
    31141 cgacaatggc attattacta atgcgatgat taaagattta actgcagata agattacagc
    31201 aggtacaatt gatgctaatc gtataaatgt cattaattta gatgctagca acatcactag
    31261 tggcaggatg tcagcagatc tgatacgtag cggcgtttta atatcgcaaa acggtgcaat
    31321 gttagcagat cttaatacag gacaaattga attttacaca gatagtccag ctatcaaacg
    31381 tattactgca ggttatccta accaattcgt taaatttgca acaggtaatg ttgaaggaaa
    31441 gggcgcggct ggcgtaacgg ttataggttc taatcgatgg ggaagtgaat cgtcaaacga
    31501 cggtggtttc gtaggtattc gcgcttggaa tggtaaagac atcgatactc ttgatttagt
    31561 tggcgatagt attcacttag caagctctgc gtacactaac gctgatggtt gggaaatcat
    31621 tactttgcca aatcagctat caatagatgc acgtaacata aatcatcgtg taacgtctaa
    31681 gataaaagta ggagatattt ggctatggaa aaatacatca acttatacaa gcatgaaaga
    31741 tacaattaat atgattattg ataacttgca gctactacac aataataaaa ccactgaaaa
    31801 aggctacagt tatactattc caggaaagat ttaggagaaa aaatggaaga aaaaataaaa
    31861 tcaaatttag ctttacaaat agcgcaatta tctttagata aagcaactct acaagctcaa
    31921 aatgagcaat tacaattaca aattgctgag ttaaatcagc aattagatga gataactaag
    31981 ccaggagcaa agtgaggtga gtaacatgaa acaatggaac attgttggaa aaccatttcc
    32041 aatctatcaa gatggagtgg ttctaaaaac ggatgtacgc ttacaaggcg acgatggtac
    32101 atatttacca caagagctaa ttggtaatca tgttgataag gataatcaat cactgataaa
    32161 acttgtactt gaaacttttg caaaagaaaa tgtcgttgat ttcgcaatgc ttgagtctgt
    32221 aaaagatatt gagcaactaa aagctgacaa agtaacttta atcaaaaaaa ttgcagaggc
    32281 tgatgaggca atttctaaga tgtcgcaagc aacaaaagat atgcagctta aagttgataa
    32341 agcagttgtc gagttaactg acttagtgac atcaagtgta tctagccttt ccgtgccgtc
    32401 tacatcgatg gaatcaagct aatggaacta acgttgaata ccgttataac actatattat
    32461 ttaataaaaa aaggagaatt taatatgaca tatactttta aaaaagatca tcttattgtt
    32521 gacgtttggt accgtcgtgt cgcgaatggt atttgtgatt tcgaagcagt acctaaatta
    32581 tttaatttac gagatgttgt gcttgagtta cttagtcaaa aagttactga taaagaatca
    32641 gagtgaggta acttaatgat agattttgtg caagttggcg cttttgcagg tgctgcttta
    32701 tctattttgg gagtatgggg atttattgtt aatcccttta aaaaagcaat ggaagctaac
    32761 gagattgcta tggctcagct caaggactca ctcaaagaac tagcatacga gctcaaaaat
    32821 atagatagag accgagagat tacaaaagct gttattgatc gtcacgagga gcgtttaggt
    32881 cgtgtcgagg atgatatcat catcaataaa gagcaaatca aaacattatt taataaggag
    32941 aaataagatg attaattgga aagtaagaat taaaaacaaa gcattttggt cagcaattat
    33001 tccagcaata tttttagttg tacaagcagt tgcaaatgtt tttggttata cacttgaact
    33061 tagtgattta ggtaataaat tgttagtagt tgttaatagt gggttttcag tacttgttat
    33121 tgcaggtatt gttacagacc ctactacaca aggagtatca gatagcagcc gtgcgctatc
    33181 ctacacagag ccaaaataat aggaggataa aatgaaagca atcactaaaa tagcaatggt
    33241 actagcaata gcaatactgt acataccgct tgcagtggtt gcttttttta gttatccgat
    33301 ttatttactt tttggaaagg aggagtaaat ggcaacttat caagaatata aaagcaggtc
    33361 aaatggtaac gcttatgata ttgatggttc gttcggtgca caatgctggg acggttatgc
    33421 agattactgt ggatttttag gtgtaccgta cgcaaactgc acaaatacag gatacgcaag
    33481 agacatctgg gaacagcgcc acgaaaatgg tatcttaaac tactttggcg aagtagagac
    33541 tatgcaagcg ggagatgtcg cgatttttat ggttgttgac ggtgtaacac cttacagtca
    33601 tgtagcaatt tttgacagcg atgcgggtgg aggctatggt tggtttttag gacaaaacca
    33661 aggtggacca aacggagcat ataatttagt aaaaatacca tattcagcga cttatcctac
    33721 tgcatttaga ccaaaagttt ttaaaaatgc agttactgtt acaggtaata ctggcttaaa
    33781 taaaggtgat tattttatcg atgtatcagc ttatcagcaa gcagatttaa caacgacttg
    33841 tcagcaggca ggtactacaa aaacgattat caaggtatcg gagtcaatag cgtggctatc
    33901 tgacagacat caacaacaag caaacacaag cgacccaatt ggctattacc actttggacg
    33961 ttttggcggt gatagtgctt tagcgcaacg agaagcagac ttatttttgt ctaacttacc
    34021 aagcaaaaaa gtatcatact tagtcattga ctatgaagat tccgcaagcg cagacaagca
    34081 agctaacaca aatgcagtta ttgcatttat ggataaaatc gctagcgctg gttacaagcc
    34141 tgtttactac agctataagc catttacact taataatgtt gattatcagc agattattgc
    34201 taaataccca aatagtattt ggattgcagg ctatcccgat tacgaggtgc gctctgaacc
    34261 actgtgggac tattttccat ctatggacgg cgtgcgctgg tggcaattta caagtgtagg
    34321 agtagcaggt ggtttagata aaaatattgt attattagca gatgatagta gcaaagtgga
    34381 tatacctaag attgacaaac cacaagaacc acaaagccag cttactttta atcaaaagct
    34441 agatactaac actaaattag acaactcgaa tgtaccttac tacgaagcga ctctaagcac
    34501 agattattac gtagaatcta aaccaaacgc aagtagtaca gataaagagt ttatcaaagc
    34561 aggtactcgt gtgagagttt acgaaaaaat caacggttgg tcacgtatta acgcaccaca
    34621 atccgaacaa tgggtagaag atgcttactt aattgatgca acagatatgt aaaccaacag
    34681 agcgacataa atgtcggtct gttaatggtg taagttacac cgcaactaaa aaaccagttt
    34741 aggaggtatt attttattgt taataataaa tattactgtt tttcttgact tagcaaataa
    34801 aaacaggtaa tataaagata caaataacga caaaaaactg cgcctctgtt gttttgtgct
    34861 cttgttgttt gtattgctgt acacctatcg ccgtaggtga cgaacaaaaa atgtaagagg
    34921 ggactccaac ctcagaaaag cacagtttgc cggctgtgct tttatttttt gaacataaaa
    34981 aagagcgggt ttgccgaccc actcttaaac aaaaaatgta atgtacgtac tattttttgt
    35041 gtaaatagtc acgtacgata tcagctaagc aattcgcagt gagagtaacc acaaaattgc
    35101 taactaatgt gaggaggaaa aattccatac tccaacctcc tttttaaaga tttgctattt
    35161 gattagttag ctctagccca tctaagctac tagagcttat ttgttgattt tattatatct
    35221 gattttgcaa ttttaaaaaa ggtcttagct gctaaaaaaa acaagaactt ctagtattat
    35281 aagacaaaac cgctcagatt aacttctggg ctgtttttta ttgcaaaaaa tttcgcattt
    35341 attgacaaaa agttcaagac atgtcataat gagggtggtt atagaagata aatttcgttc
    35401 atttatcatc atttctaacc caacgtcttc gttgttcgtt gaacccgtag tgatacgggc
    35461 gtatgactga aagcacatca tacggcttgg cagagcttaa gaactgttct cttgcgataa
    35521 gcctaagaag cacaatagag agttagagtt ttgcacctct aatcgttagc cctgaccgga
    35581 ggatatttcc ggtccgtgct tttttatttt gtagaaagtt ttttgatagt ggatctcaaa
    35641 gaaatagtta atgattatga gctaaatttt tgtgggaaaa ggtgtaaagt tgagactaat
    35701 tttaagcatt tacccgaatt tatgatttta tttgatataa gagatttgta tcatcttcta
    35761 ggtattcata agttgaaaac aaagtatcgc gcaacaaatt gggttgaagc tgtgaaagca
    35821 gatgttttcc tcttatcgaa ttattcaaaa catccaaatt ttagagaagt tcttcctaga
    35881 gtcgataatt ataatttttt atatgaaata ttctatcagt ttagagttaa cgtctgtatt
    35941 ttagataagg atttaactaa aaatacaatg aaattgagtg ttgtttttta taaagacaac
    36001 aagaagaaat tagttgttgt aggattaaaa agagatgaga caggggtctt taggccagct
    36061 acgttgcatg agagccgaaa caacccctac aagcgaattc ggcatactgc tataaaatca
    36121 ataacttgga tttagaacat accttttgtg ctgattacct ctattgacac actcgcataa
    36181 tcatgagata atcacagtag caagaatcgc ctgaaagtag cagtagatgc tgtcaaagaa
    36241 aaactgaaat aaatattaac cgctcattta atgagcggtt attttttgtc tatcagaaca
    36301 gaaaaattta aaattgtcta tttttaggat tttttatcga atagataagt ggaggataaa
    36361 atatgttata tatagatgag tttaaagaag cgattgataa aggctatatt tcaggggaca
    36421 cagtagcgat agtgcgtaaa aacggaaaga tatttgatta tgtgttacca cacgaaaaag
    36481 tgagagatga tgaagttgtg gcagtagaga gtgtggaaga cgtactcgca gaactgggta
    36541 atgttcgttg tgatataagt agctgtcaat agtttgcccc aactaacaat atactttttt
    36601 gtggggcgaa tttggggcat aagtttaaaa ctaatctatg ttaacaatga tacgactttt
    36661 atgtttacta ctcataacga cttatataga tatgttttag accttataat gccaaccgaa
    36721 aaacctaact cttaaagacg ctgttaaata attcgtctag aaaaaccttg tcatatcaat
    36781 gtttattgat agcgacaagg ttctttttta tatccaatac tcaataaacg actagagagc
    36841 tcaaaggaat ttagagtttc aaatgtgtta gagcataccc ttgaattatc ttaactgtga
    36901 tgttataatt cctaaaagat aagttaaaag agaggttgcg aaagagaatg tcagaaaaag
    36961 ctatactatt aattatcctt attgcttttg ttattatcgc tattataaag atagctctgt
    37021 taaagttata tatgaatacg ataaaaccta aagtagataa tgaaggaaac atcataacaa
    37081 agcctgtaaa ggatgattta acacccgagg aacgtgaaga acgtgaattt gataaagatt
    37141 ggtaaaatca atataaaaga gcaaacaagg tttgctcttt tattattttt aaaggtaatt
    37201 aagttgtcct cgaaaatcct ccaatgattg atagccttta tcagccatta tttcttttaa
    37261 ctctctgctt acacgctgaa aaatttctgg tccttctttt tgtaaagcgg ttccgatttg
    37321 gaccatacta gctccacata gaatgtgttc aaaggcatca cggccgtttt taacaccacc
    37381 tgttccgata attttaattg aaggatttaa acgtttgtag aaagcatgga cgttagctaa
    37441 ggcggtaggc ttgataaaat caccgccgat acctccaaag ccatttttag gctttataac
    37501 gacagtttca tcatcaataa caagtccatt tccgatagaa ttaacacagt taataaaagc
    37561 taaaggatat ttgttaaaaa ttgtagctgc ttgatcaaag tgaacgatat caaagtaagg
    37621 tggtaatttg atgcccaatg gcttttgata atagctaaag atttctgaaa ggatgaggtc
    37681 agtcatttca aaatcataag caatttgagg ttttccagga acatttgggc acgataaatt
    37741 taactctatc aggccattgt aactactgtt ttcaaccttt ttaagaataa tatgtgtctc
    37801 ttctggtgat aaacctacta atgataaaac gtgatttttg ctattgtctt ggtcttgtaa
    37861 ttcagtcacg aaatcaagat aatagtcaat acctttatta gggagtccca tagaattaat
    37921 tgatccccag tcagtatctg cataacgtgg ttgagggttt ccctctctag ctttaagggt
    37981 acctgttttt gtaacaaagg aaccagcttc agaattttca atagctaata actcttcctt
    38041 agtcatacaa taaatacccg ctgcattcat caaacaattg tcaaaactaa aaccagcaat
    38101 ttccgttttt agcgatacca taatatcctc ctgaagataa atttttacta ttttatcact
    38161 tgattaacag agcggcaaga a
//
LOCUS       DATRME010000011        35317 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRME010000011 DATRME010000000
VERSION     DATRME010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35317
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(174..791)
                     /gene="nrdG"
                     /locus_tag="VBC23_000578"
     CDS             complement(174..791)
                     /gene="nrdG"
                     /locus_tag="VBC23_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6842548.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(864..1355)
                     /locus_tag="VBC23_000579"
     CDS             complement(864..1355)
                     /locus_tag="VBC23_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6842549.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(1364..2296)
                     /locus_tag="VBC23_000580"
     CDS             complement(1364..2296)
                     /locus_tag="VBC23_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6842550.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(2309..2452)
                     /locus_tag="VBC23_000581"
     CDS             complement(2309..2452)
                     /locus_tag="VBC23_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842551.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(2527..4725)
                     /gene="nrdD"
                     /locus_tag="VBC23_000582"
     CDS             complement(2527..4725)
                     /gene="nrdD"
                     /locus_tag="VBC23_000582"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6842552.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(4824..6365)
                     /locus_tag="VBC23_000583"
     CDS             complement(4824..6365)
                     /locus_tag="VBC23_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842553.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(6616..6933)
                     /locus_tag="VBC23_000584"
     CDS             complement(6616..6933)
                     /locus_tag="VBC23_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6842554.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(6959..7378)
                     /gene="ruvX"
                     /locus_tag="VBC23_000585"
     CDS             complement(6959..7378)
                     /gene="ruvX"
                     /locus_tag="VBC23_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6842555.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(7378..7644)
                     /locus_tag="VBC23_000586"
     CDS             complement(7378..7644)
                     /locus_tag="VBC23_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6842556.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(7846..8244)
                     /gene="spx"
                     /locus_tag="VBC23_000587"
     CDS             complement(7846..8244)
                     /gene="spx"
                     /locus_tag="VBC23_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6842557.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(8460..9599)
                     /gene="recA"
                     /locus_tag="VBC23_000588"
     CDS             complement(8460..9599)
                     /gene="recA"
                     /locus_tag="VBC23_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6842558.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(9673..10932)
                     /locus_tag="VBC23_000589"
     CDS             complement(9673..10932)
                     /locus_tag="VBC23_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO6842559.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(11021..11572)
                     /locus_tag="VBC23_000590"
     CDS             complement(11021..11572)
                     /locus_tag="VBC23_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6842560.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(11595..12185)
                     /gene="ruvA"
                     /locus_tag="VBC23_000591"
     CDS             complement(11595..12185)
                     /gene="ruvA"
                     /locus_tag="VBC23_000591"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6842561.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            complement(12187..13419)
                     /locus_tag="VBC23_000592"
     CDS             complement(12187..13419)
                     /locus_tag="VBC23_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6842562.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(13451..15424)
                     /gene="mutL"
                     /locus_tag="VBC23_000593"
     CDS             complement(13451..15424)
                     /gene="mutL"
                     /locus_tag="VBC23_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6842563.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            15650..15853
                     /locus_tag="VBC23_000594"
     CDS             15650..15853
                     /locus_tag="VBC23_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6842564.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(15910..18486)
                     /gene="mutS"
                     /locus_tag="VBC23_000595"
     CDS             complement(15910..18486)
                     /gene="mutS"
                     /locus_tag="VBC23_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6842565.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(18543..18980)
                     /gene="argR"
                     /locus_tag="VBC23_000596"
     CDS             complement(18543..18980)
                     /gene="argR"
                     /locus_tag="VBC23_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6842566.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            19202..20893
                     /locus_tag="VBC23_000597"
     CDS             19202..20893
                     /locus_tag="VBC23_000597"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6842567.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            20981..21289
                     /locus_tag="VBC23_000598"
     CDS             20981..21289
                     /locus_tag="VBC23_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6842568.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(21316..22188)
                     /locus_tag="VBC23_000599"
     CDS             complement(21316..22188)
                     /locus_tag="VBC23_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6842569.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(22296..23240)
                     /locus_tag="VBC23_000600"
     CDS             complement(22296..23240)
                     /locus_tag="VBC23_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6842570.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(23230..24981)
                     /gene="aspS"
                     /locus_tag="VBC23_000601"
     CDS             complement(23230..24981)
                     /gene="aspS"
                     /locus_tag="VBC23_000601"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6842571.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(25074..26354)
                     /locus_tag="VBC23_000602"
     CDS             complement(25074..26354)
                     /locus_tag="VBC23_000602"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6842572.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            26574..26756
                     /gene="rpmF"
                     /locus_tag="VBC23_000603"
     CDS             26574..26756
                     /gene="rpmF"
                     /locus_tag="VBC23_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6842573.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            26772..26921
                     /gene="rpmG"
                     /locus_tag="VBC23_000604"
     CDS             26772..26921
                     /gene="rpmG"
                     /locus_tag="VBC23_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6842574.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            27201..27827
                     /locus_tag="VBC23_000605"
     CDS             27201..27827
                     /locus_tag="VBC23_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO6842575.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            27839..28177
                     /gene="cadX"
                     /locus_tag="VBC23_000606"
     CDS             27839..28177
                     /gene="cadX"
                     /locus_tag="VBC23_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO6842576.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            28228..29643
                     /locus_tag="VBC23_000607"
     CDS             28228..29643
                     /locus_tag="VBC23_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842577.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKYNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDEKLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILFELGGNDIPSTFRQENI
                     DPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILERMYKENIPKSYLTSVPL
                     VISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDIAELYQKYSKEELANLG
                     INIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLEPTRFSLEDKKRIEEAA
                     AQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYYFQEQLNKELLSMAHAL
                     DNVQQAFIKLLSEEEIRKFAL"
     gene            29963..30412
                     /locus_tag="VBC23_000608"
     CDS             29963..30412
                     /locus_tag="VBC23_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6842578.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            30614..30934
                     /locus_tag="VBC23_000609"
     CDS             30614..30934
                     /locus_tag="VBC23_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842579.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            30934..32034
                     /locus_tag="VBC23_000610"
     CDS             30934..32034
                     /locus_tag="VBC23_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6842580.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            32031..32636
                     /locus_tag="VBC23_000611"
     CDS             32031..32636
                     /locus_tag="VBC23_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842581.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            32842..33447
                     /locus_tag="VBC23_000612"
     CDS             32842..33447
                     /locus_tag="VBC23_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6842582.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            33440..33688
                     /locus_tag="VBC23_000613"
     CDS             33440..33688
                     /locus_tag="VBC23_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6842583.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            33778..35043
                     /locus_tag="VBC23_000614"
     CDS             33778..35043
                     /locus_tag="VBC23_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6842584.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT        11520 a         6925 c         5570 g        11302 t
ORIGIN      
        1 tataaagttg attacttaac agtaatgtca ttgtatatac ctttagattt caaaaatact
       61 tgttaaatta acaataataa acgtcaacat cttgtcacag actgtcaaat acttataaac
      121 aagctgtgga taaacataac tttcaatttc tatttaaaag ttaatttttg acactaaagt
      181 aaataatctc gtttgacttg ttcataacta ttgtcaccat cgtttaatcc ttcccaaatc
      241 acgacttcac cttcttttaa agatttttgt acatcaatga ttctttgatt tgaagatcct
      301 ctaaattgta gcattaaatt tcgcttactt tgatcaaacc taccatccac taaaatatca
      361 atcaaagata acatctccaa tttatcttga gtctccagca tcatttcttc ccaagtatac
      421 cccgtccatg accaaatatc tttttctggc aattcacgtc ggatacgctg aagaagtggc
      481 agcaaaatac ctgtatttaa gaacggctct ccacctaata acgttaaacc ttggacgtaa
      541 ggttgtgcca aatctttcat aatttgatct tcaagctctt ttgtataagg gattccggct
      601 cgaaaggacc atgtagcagt attataacac cctttacagt ggaacataca tccagctacg
      661 tagagtgaat ttctaacacc ttcaccatct acaaaattaa aagctttata atcaatgata
      721 tggccatgac ttaattcttc gcttttccac tccccaggct ttggagtatt ccaacttgct
      781 tcttctgaca tttctaacct ctctattgat atgaaaagaa ccagaactaa ctggtctttt
      841 tattattatc ttaaagtact atcttaggtt atccaataac gctctgtctg atgacgacaa
      901 tcttctaaaa taccaccgtt agctaatatt actgacttgc tggcatcatt gtctacatgg
      961 catgtcacaa gtatttcatt gatatttttt agatgtgctt gctcaattcc tagcttcaac
     1021 tgttctttag cataaccttt accacgctgg cgaggtcgta tggaataacc aatatgtcca
     1081 cctttttcta aaagagtatc atttagtctt aaacgtaaat ttaagaaacc tatagcatta
     1141 ttgtcattgt caaaagacac aaactgaata tatggaacaa aattatcagg aacacctatc
     1201 cctaactcca tatttaggtt atgatctagc cagtcttcaa aatgcgtagt tggttcataa
     1261 aaaccaccat ccattgaact actttctttt tgaaactctt tatacatggc taaaatttct
     1321 tctttatctt ctaacactgg gcgccgtaat ttcatattgt ttcctattcc attatcttca
     1381 agttaagcat tccaaaagga gcattcgctt tattaatctg gtcacaaata aaatgactca
     1441 ataaactaga ctttggtgat ataggattga cacctgtagt gatagcttga ataaaagcat
     1501 caatcattgg ttcaaaacca cgtttctcta gagtgcttgc ccaagaacca aaaccgatag
     1561 cacgacggtc aaagccatca ataacagaaa gatttgacaa atcatctaat gaataggtta
     1621 cttctggcga ctctatttca attatttctc gacgagaccc cgactgcagg ttcatgcttg
     1681 cttctattat ctcactgtct gtttctagcg ttactgatac ttgtaatatc ttattcccat
     1741 cacgtttagt cacaaagtat cctcttttaa caacattatt tgtcaaaaat agcgccgtat
     1801 ctaaaggatg gataaacata tcaaaaagct tatattgaaa atccgctggt gcattgacag
     1861 catttttaaa ggtgcgaata tggtttttat cttctacttt tttcatttcc ataatacgtg
     1921 gtgcaaagcg acgattaaaa cctgccatta aaaatgtttt atgatccttc gccaaatcat
     1981 acaatgcttt agttgaagta taatcttctg tcaaaggctt atccataaaa actggaatac
     2041 ccttttttaa aaaaagcttt gctatatcaa agtgagcact agtcgcgaca tgaatgaaga
     2101 ctccatccaa aggatgctcg gctagactat ctaaactgtc ataagctgta gaagaaccaa
     2161 aaagcatatt aacttcttct aaaatctgtt tttggcgtgt aaaaagatgc cagtgcacac
     2221 ctgtcacttg acgcatataa ggtagataag ctttctgtga aatacctcct aaaccgacaa
     2281 ttccaagttg caacatagca tcctccttct attttttctt tgccttttcc aaaaattgtt
     2341 tccttaaatc ttttagacgt gatttttgat tagcattctc tccagtagag tgcttctcat
     2401 ggaacttctg tacttgtgct tgccctttgt aatctaattg gtatttcccc atatcttaat
     2461 aactccttct attttatctt cttattttaa catataatag accagaggtt ttaatagagg
     2521 ttttaattaa ttaccttgat ttttaatact tgatccgttc atatgtttta cacgagcaga
     2581 gatttcctta tgacgaccat taaccatcgg acgagcctgt ggattaccta agtaaccaca
     2641 tgtacgtttg acaacatcta ctgtcttagg gtcgctgtta ccacaatttg gacaagtaaa
     2701 gcctcgatct gtcggtgtaa aatccccttc aaattgacat tggtaacact tatcaattgg
     2761 tgtattagta cctagataac ctacccgatc ataagcatag tcccaaactg cttctaaagc
     2821 ttttggattc tgttgcaaaa cagggtattc acagtagtga ataaatcctc ctgatgcacc
     2881 cgtttcagga tagattttct caaaatctag tttttcaaaa ggtgttggat tcttacgcac
     2941 atcataatgg aatgaattag tgtagtattc cttatcagta atatcagtta ctataccaaa
     3001 tttttcagta tctaaacgac aaaaccggtc agtcaagctc tccgatggtg tcgaataaac
     3061 tgaaaaatga taatcatact catcagacca atctgcacat aactgcttca tcttacgaac
     3121 gatgtctaca gtaaatgctt tagcttgatg attatcttcc cagtcaccac cataaaaaac
     3181 actcgccacc tcatataaac caatataacc aagagacacc gttgcacggc ggtgattaaa
     3241 gagtcgatca acattctcat attttcctaa acgttctcca aatgctccat actggtaaag
     3301 aatgggcgca tttgctggga tagcctcttt gacacgttcc acacgataaa ccaaggcatc
     3361 tctagcaata ctcatacgtt cattaaatat ctcccagaat ttatccatat caccgttaga
     3421 ctccatggca acacgcggta gattaactgt tacaactcca agattcatac gaccagatgt
     3481 tacatcctga ccatttgcat ctcgccaccc ttgtaggaag gaacgacagc ccataggggc
     3541 tttaaaagag cccgttaagt caataatttt atcataagaa aggacatctg gatacatccg
     3601 ttttgtcgca cactctaatg ctaattgttt aatatcataa ttggggctat tttcttctaa
     3661 attaagacct tttttaagcg taaaaattaa ttttggaaaa atagcagtac gatgctcgct
     3721 tcctaaacct tgaatccgaa ttttcaaaat cgctttttga atttctcgtt caaaccaatt
     3781 agttcccaat ccaaaaccaa gcgaagtaaa tggcgtttgc ccatttgaag taaataaagt
     3841 attaatctca tactccaaag attgcatggc atcatatata tctttttgtg ttttagcgcg
     3901 cgcatagtcc tcttgtttat cttcaataac ccattctttt gcatctttta aatgtttttg
     3961 ataatttaac tgtgcataag gagctaaaaa ctcatcaatg cgatctgctg tacagccacc
     4021 atattgactt gaagcaacgt tcgcaataat ctgtgaaatc tgagcagtcg ctgtctgaat
     4081 agattttgga ctctcaactt ctgcattacc aatcttaaaa ccatttgcaa gcattccctt
     4141 aaaatcgatt aaacagcaat ttgtcatggg agtgtatggg ctgtaatcta aatcatggta
     4201 atggatatca cccttttgat gtgcatttgc tacatgagaa ggtaacattt tcagaccaat
     4261 tgatttccct acaatacctg ctgttaaatc acgttgggta ttaaacacat cagagtcttt
     4321 attagcattc tcattaacaa cagtttgatc tttattaatt aattttccaa tagaaaaatt
     4381 gatatctgta gcctgtgaac gctcaaaatc gcgttgggta cgataattga tatattcttg
     4441 tgcaatagcg tactcattag cttccaataa tttatgttca acgatatttt gtatttcata
     4501 tatcttgatg ttatctttaa aacgattgta gatctcagcg atgacatgat ctgcaatcat
     4561 ctcaagttta gcctcaacta aaggagacat tttgataact ttattactag cttttaatag
     4621 agccgtgtag attttatact tatcaaagct gaccaaacgc ccgtcacgtt taatcacttt
     4681 gatatcagat tctgtcataa atcgttctct ttccaatacc atcatgtgaa atatcctcca
     4741 gtgacaatta tagcatgcaa agaaaaaaaa tcaatatatt gtgtcaagtt ttttgctcag
     4801 acacaatata ttgatttttt aatctatttg aaacgatata gaacaaaatg agtgatattt
     4861 gatagaggaa cttcttggta gtgcttctta atatcagatt tcagttcatt tggtaacttc
     4921 tcatttttat ttaagataaa gtatttagcc ccatgctgaa gcagctcgtc cttcaaaatt
     4981 ttctcattat ttttttgcgc tgtattaata tgtggcaaaa caaattgcga agcagatttt
     5041 aaccgagtct ggctataaat gctagcaact ttgtcccaaa cataaatctt accatctcta
     5101 tcaatttttg tagtcaaata atgtgaaatg tcacgccttt ctttactaag agtaacattt
     5161 tgataagtat tataaacaag taaccctatc aataaggaaa ctagtaagag gggcaaataa
     5221 tatgttttct ttacaaaaag gtctacgata agagatttac cagagaaata ctgacgtcta
     5281 tgtgaacgcc tgtcaatcac tttttggtaa agaatagtaa ttttattact cgttagcaaa
     5341 agaccaaatg gtaaagcagg taaaaagtgg taaaggttaa aatctcttga aaaaatggcc
     5401 attatagcgt aaataataaa aatacaggca ataaagttca agccgatttg ataagatgct
     5461 tttttacgat tttgaataaa atcccatagt ccaaagaggc aacctgaccc tagaaggaca
     5521 acaatttgaa ttgctaaact aagaaggaat gagtggtttg tcctagcgaa atacgtaaag
     5581 gggtaaaaaa tagctttgtc aatataagaa gagataatct gtagattaaa cataaagtaa
     5641 ccaacagtat aagcaattaa aatcatacca aaaacaacgc ataaaaactg atagaaccct
     5701 cgtagtgact gcttttgctt aatattggtt gctgttaaga gaacaaaaga gcaaagccaa
     5761 aaaatcagtg tttggggatc tataaatagt gagaatgctg ctaaaatacc ataaaatata
     5821 aatgcttcat caacgatatt atcgacacaa aatttaatta aaaaccatag cgaaatgagc
     5881 ataaaaggta atgcccactg tattgggtac ataccaccga atcctaagac aatattcatt
     5941 atatagaaaa tcaacgtcat gcctatcgag acaatttcac tttgtgccac ataataaact
     6001 aatttataaa aataaattcc agatacatag taagcaataa actgtacaat aaataaccag
     6061 atactagacc ctaaaaggta acttagagca atcgttacat agtataaaaa tcctcctgta
     6121 gcaaaaacat cactataagg gatctgtcct ttggtcatca tcaaacctgt atagagattc
     6181 tgagtctgca aaccatttgc aaaataagtc aataaaggat ttgcaattgc taagagagtt
     6241 gacaaaacac tatatagtaa aattgaaaat cgactagatt tcttcattct gatagccgtt
     6301 cgtttactca ttgatgggct atttttatag ctcactccaa ttggtttttc taggttttct
     6361 accactattt ttctcctcat atttacatac taccagtata tcagaaaacc ttatttagtc
     6421 aatttagcca acacttgacc gtaacgaata atatccctaa agtagtggta tcttctagac
     6481 atatattgtt tgagaaattc ttgttcttct aatgttagct gagtatctga cattaacatc
     6541 acctcctttt actacatcat cactattatt attcgaaaaa aaaaacgact gaacaattgc
     6601 tcaatcgctt caatattatt cttcatctag aaagctgttg aaaacttcct caatcatgtc
     6661 ccattctgca tctgaatctt ctggtatagg ctgaaggtct ccttcagttc catcagcatt
     6721 ttctgtaaat gagtaagctt gaatctcaat ttcaccttgt tcgtcttctt ctgcacctgc
     6781 aggaacgagg agaacatagt ttttgccaaa ctcttcacgt ccatcaatag ttagtaggat
     6841 ttcaaaaagt gtttcgttcc cattctcatc tacaagagtg attacttcat gttgatgttc
     6901 atggttatga ccgtggttat gattatgtgc cattgtttct cctttttatg ccgaatgctt
     6961 aaaacatgcg gtctaaataa ttttgtaaga ttagttgagc tgctaattta tcgataactt
     7021 tcttacgctt accacgacta atatcagctt gctcaactag catacgctct gcttgaacag
     7081 tcgttagacg ttcatcttga tattcaactg gaagattgaa taattcagtg attttatcgc
     7141 catatacctg actagcctct acacgaggac cacttgtatt attcatattt ttaggtagac
     7201 caactacaaa tttatcaact ttgtactctt ttactaactc tgctaagcgg tcaaaaccaa
     7261 aatttccaga ttcttcatcg atcttaatga tttcaagacc ttgtgcagtg aaaccgagtg
     7321 gatcactaat tgcaacgcca acagttttcg agccgacgtc aagtcccatt attctcatta
     7381 caaatctatt ccatttcctt gtaaataata acgaacgagc tcttctacaa tctcatcacg
     7441 ttcatattta cgaatttgat tgcgagcatc attgtaacga ggtacataag cagggtcccc
     7501 actcaatacg taaccaacaa tttgattgat gggattgtaa cctttttctt ctaatgaacg
     7561 ataaacagct gttaatgttt cactaatttc tactttgtta ctatcatcta atctaaaacg
     7621 aacagtttca tctgtaaatc ccatacctac accttctttc ctttaattct ttcacttatt
     7681 ataacttaaa taaagtattt gtacaagaaa ttgataaaca ctatattaga aaatcaaaaa
     7741 gggagcacaa gaaaccaaga ggtgattctt tgatttcttt actccctacc ttataggtga
     7801 tttttacagg ttatcatgtg actctcactt atgcttatct aaagctcaaa gtgctgcacg
     7861 aagacgagct tctgcatttt caacgttacg aatagagcga ggtagaaaag cacgaatatc
     7921 atcttcctta taacctactt gaagacgttt gtcatcaatt agaattggac tctttaaaat
     7981 acgtggattt tcttgaataa gatcaataac ttcatttaca ctcaactcct caatattaca
     8041 attgagcgcc ttagcataac gatttttaga agaaacgata ctttcaatac cactttcagt
     8101 ttttgttaat atttctaaaa tttcatcacg tgttagtgat tctttaccta ggttttgttc
     8161 tttatatgga agttgatgag catttagcca agtttttgct tttttacaac ttgtgcaact
     8221 tgaaatagta taaatcttaa tcatgtgcct tctcactttc tatcccatga tagttatagt
     8281 ttaacatagt cacaaaaatc aaacatggga cttaagacct attttttatc aatttactaa
     8341 taaaaataaa tagattttag ggatttttaa gcttattttt agtataaaaa taagttccaa
     8401 ccactgtcag aacttatttt agttacattt attcaagttc aagatgtata gttgctattt
     8461 tattcctcaa tttcaatagc attgtccaaa tctaaaacaa gatcatcagc ttcatttttt
     8521 tcttcaacga gctcagactg aactggcgaa tcatcttcag tcattccaaa gtgcacgcgc
     8581 actttatggt caatttcatc aaaaattgct ggattatctg ctaaatattt tttggcattt
     8641 tcagaacctt gtccaatttt ctcaccatta tatgagtacc atgcacctgc tttttggata
     8701 atatccaaat cactagcaat tttaattaac tcacccgtgc gagaaatacc ttcaccatac
     8761 ataatttcga caaaagcttc acggaatggt ggagcaactt tatttttaac aactttgatt
     8821 tttgtctctt taccaacatt atgatcttta tgctcaccag tccccttgat ttgagtatta
     8881 ccacgaacat ctagacgaac cgatgaataa aatttcaaag cacgaccacc aggtgttgtt
     8941 tcagggttac caaacataac gcctactttt tcccttagct ggttgataaa aattgcgatt
     9001 gtctttgtct tgttaatgga tgctgacaat ttacgcattg cttgactcat catacgagct
     9061 tgcaaaccaa cgtggctgtc accaatatca ccatcaatct cagcacgagg aacaagggct
     9121 gcaactgaat cgacaacaac taaatcaact gcgcctgaat caatcaattt acctgcaatt
     9181 tcaagacctt gctcacctga gtcaggttga gacaagagta actcatcaat attaactcca
     9241 agagccgcag cataggctgg gtcaagagcg tgctcagcat caataaaggc agcaatccca
     9301 ccttcctttt gggcttgagc tacggcatga agggcaaccg ttgttttacc agatgactct
     9361 ggtccataaa tttcgacgat acgtccttta ggatatccac ctgctcctaa agcgatatct
     9421 aaagcaagag aacctgaact cattacttga accttttgtt cagctcgctc acctaagcgc
     9481 atcactgcgc ccttaccaaa atctttttca atatttttca aagcatcatc taaggctttt
     9541 cgacgttcat caccgaattt cttagtaatt tcttctgctt ttttcgtttt tttagccaat
     9601 gtcttctcct tatatttcta tgaatctagg ttgattcaaa tttagaaatc gttctactaa
     9661 ttataacaaa tattatatct ttaataaagc cctacgcacc aggtcaaaag catgtaaagt
     9721 actgatatga cggacatctg agcggctctt ccctccaatc gatactttaa tagaagaaac
     9781 gccttcaggg gttgcaatcc caataaaaac tgtacctgca gggtatcctt ccaactcatc
     9841 aggaccagca actcccgtta aaccgatacc aaaatctgct tttaaaagct ggcgagcttg
     9901 ctgagccatt gcctcagctg taaaatgact gacaactcca tattcttgca attttttctt
     9961 agggatgccg agaagttgtg attttgcttc catactatag gtcgtaaagc caccattaaa
    10021 aacttgcgaa gcaccagaaa attctgctaa ctgagattga aataaccctg ctgttaagct
    10081 ctctgcggca gtaatcgttt tattttgtac tttcaataat tctaaaacag tacgtgccat
    10141 actattatta tcaccatagc catataataa atcttttaat ttacggttat ctaatgtctt
    10201 aagagcaagt atttcttttt ctaagctatc taatttaaag tcggcttcat tctgattttc
    10261 agctttcgtt gataacctta aagttacctc accaatcttt gcatatggtg ctatagttgg
    10321 atctgtctga ttcttaatta tgtcttctaa gattgtgact aactgactct caccgatacc
    10381 aaagaatctc aatacacgtg aatataattt tgtgccatta tttgacaaaa taggtaccac
    10441 ctgctccata accattggct ttaattcact aggcggacct ggtaaaacca tatattggac
    10501 tcccttagat gtaacgatac caccaacagc tagaccagta aggttttgta gaggtattga
    10561 gccttcaacc aattgagctt gtcgttcatt atttggcgtt ctagcaaact gaggacgact
    10621 tgcaaaaaaa cgatccaatt tttgactagc taataaatcc actgttaatg atttacctaa
    10681 aaatttagcc aaagtttgtt tcgttaaatc atcctctgta ggtccaagtc caccacatag
    10741 gataaccatt tgactgcgct gactagctat atctaaaagc gataataaac gttcctcatt
    10801 atctcctaca gctgtctgga aataaatatc aattcccaat tctgccattt tctccgacaa
    10861 aaattgtgcg tttgtattaa cgatctgtcc tgttaaaatt tctgttccta cagcgataat
    10921 ttcagccctc acaacttcac cctcttatct attcgaaata atgactacat tttagcaaaa
    10981 aaagtagcag aagtgctact taaaatatat cacattgata ttacttgaaa tcacatatat
    11041 tttcatggtc attaaccata cccgcggcct gaataaaaga atagaggcaa gttggaccaa
    11101 caaatttaaa accgcgtttt tttaaatcct tagataagcg ttcggataag gtagtacttg
    11161 caggcacatc gttgtagttg ttaactgagt taacctgtac tttattatct acaaaagacc
    11221 aaatggagcg atcaaaactt ccgaattctt tctgaattaa tataattgac ctagcatttt
    11281 gtcgtgttga gaaaagtttt agacgatgac gaataaccct aggattttgt aagattatct
    11341 ctaaatccgc atctgacatt gcagctacct tttcaatatt atagtgatga aaaacttgtc
    11401 tgaactcctg gcgtttattt aatactgttt cccaagataa acccgattga taagtttcta
    11461 aacatagtaa ctcaaagagt acatgatcgt catgaacagc tctcccccac tctttatcat
    11521 ggtaagctac atataatgga ttatctaaat tcacccaaga acatcgtttc atgtgaaacc
    11581 tcattacttc taaattattt catcaacatc ttcaaactag atttgatgta ttgctccaca
    11641 gtatcgttcg taccttcaaa aaaggctttt acttttttca attcagtcgc cttatacccc
    11701 aacgctaaca aagcttccat agcttcttca agattgctat tctgctcaga gctaacctta
    11761 cgactagtcg cactctcacc agaagcttct acaaacttcc ctgacaaatc aaggatcatc
    11821 tgttgtgctg ttttttttcc tatctttgga aatttagtca aatacttaat atcactatta
    11881 tcaatggcac ttacgagacc ttcattgtca tcgactgcaa taattgctaa agcggtcgtt
    11941 ggaccaattc ctgatactga aattaaatta agaaaaattt ctttttcatt ttccgtatga
    12001 aaaccaaata aaagatgtgc atcatcgcga atgacttgat gtaagtagat ggtaacttct
    12061 tgattaacgt aacctgaaaa agagtaggga tttgccacat atatcacgta tcccaatcca
    12121 gctgtttcca caacaataaa tttagccgtt attttactca attttccttt aatataatcg
    12181 tacattctat tttcctatct caatattttt cattttggta gcgtataaga ttaaaaccag
    12241 acctgctaac ataaaaatgg tcaatgttat ttgaacacct atttttcctg taaaatagct
    12301 gagcgaaacc atggcacctg ctaatacact tgcaacaggt tgtgcgatag ctaaaaatcc
    12361 agaatatgat ccaatttttc cctccaccat catctccgcc cttagaacct gactagctgg
    12421 aacataaatc atttcgccaa aggtgaaaaa cgtcatggca atcaaaatag ctccaaaagt
    12481 gtttagagaa atattaaaaa gcattccaaa accacaaatc aagcttccta atattagttg
    12541 acgtttcaaa ggccaattct ctatgaattt atttatagtt gtcattagta agacaattaa
    12601 tagagtgttc gtaaagactg ctaaactaag cattttagca cctgtgattg tatgtcctaa
    12661 cacagacact acttcaaaat tttgcttaag attgaccgag aaataattat caacctggag
    12721 ccagacaaca ctggaaccaa ttgctcctaa agtataaaca acaaacgctt tatccactaa
    12781 aacctgacta taatttttaa aagttcctaa aactccctta tcatgcttga aggttccctc
    12841 ctgtggtttt gtctcatcaa acttgaagta gaccaccaaa caacaaatcg ctgaaataat
    12901 aagaagaact attaataatt caaaaaaatg gtgatcatag aaaatcccag caatcccaga
    12961 acctagcatt acagcgatgt taaccaacca ataacctata gtgtaaacaa agcgacgatt
    13021 ctgttcgttc gtcaaatcaa tggtcatagc ttcataggca ggggaataaa attggtgagc
    13081 tatttcaatg ataaggatac ctacaaaggt caagtgtggc gtaatatgat ttggaacatt
    13141 tgcagctata gttatagccc aaccaatggt tgccagcagt gaacctacaa tcacaacttt
    13201 tttcctccct atagcatctg acaaatggcc tccatataag gtcgctacaa accctgatag
    13261 ttgagttatg atgatcaaaa ttcctgtaac tagatttcca aaatattgaa cataatacat
    13321 tgccataaaa ggaaaaatag ctgaaccaac cgtaatggaa ataaaacgca ataattctct
    13381 taactgtagc tgttttggca aagcaaaaaa ctctttcact ttttctccct tcttccctaa
    13441 caagtaatta tcaatatttt cctaaatccc tgaggcttgt atgattttct tggattcgtt
    13501 taaacatttt ctccatatca gcttttgtaa aattaactaa aacaggtctt ccatgagggc
    13561 aattataagg atttttacat tgcgctaact gatctaataa atgacgtgct gagtaatcgt
    13621 ctagcgtatg gtttgcttta atagatcgct tgcaagacat cataatagcc aactctgcgc
    13681 gatatttttt cactgatact tcattagtga gcaacaacat atcacacatt tcataaacgc
    13741 cagactctac ctcttcttct ttcatccaaa ttggatgttc ccttaaaatg aaagtattat
    13801 taccatacgg ttccaagaaa ataccaacat cttgtaataa tgacattttt tcttgtaatt
    13861 gaagaaaatc tgaacttgaa aattcaaata gaaatggtac taacaattgt tgaagactat
    13921 tatcaacctc acctatcttt tcacggtaat actcgtattt tacacgctct tgagctgcat
    13981 gttgatcaat aatatacaac ccaccattcc cttgagcaaa taagtaggtc ccatgcattt
    14041 gaccaaaaaa ttcgagctct ggaaaagtcg aactttcttc cgaatctaat ttttcaacaa
    14101 gtttgttaag tgactgttta ctactaaggt agtcctgaga atgaatgaag ttctcactat
    14161 cagcttgatc tctactagca tactttatat taggagactg cgttgctcct tgattaacag
    14221 agttttttac atcattgtca actgaaccgt cacttttgtc aaggtttgta aagttagcaa
    14281 taggttcaga tacagtatct gcatctataa aaaagtcatt tttagtccta tcgtaataca
    14341 aaccagactg ttttagtgag aagcttgttt gtataggctt atctacactt cgggtactag
    14401 ttttcgctaa attctctaaa gcatccggaa tcaaatcata ttgtttcaga ctctcagaaa
    14461 ttgctgagct tattaaactc attaattctc gttccttaga aattctcacc tcttgcttag
    14521 tcggatgaac gttgacatct gctagataag gatctatctg aatatcaatg acagcaattg
    14581 gaaatcttcc aaccatcaac tttgaaccat aaccatctag aattgagcgg tttaaaagaa
    14641 aatttttaat gtaacgaccg ttaattaata gggtaatata gtttcggtta gctcgtgtca
    14701 attcaggcaa acttacataa cccgaaattt caaaatctaa atctgcattt gaaatctcaa
    14761 tcattttttt tgcagtattt aaaccataaa tacctgcaat cgcctgtctc aaatcgccag
    14821 tcccactagt tttagtcatt tctttaccat catttatcag tgtaaaagca acttccggat
    14881 gcgctagact gagtctatta acaatatcaa taatatgagc tagttcagac tgtaaactct
    14941 tcatgtattt caatctggca ggcgtattaa agaataaatt ttcaactaaa atttttgttc
    15001 cacgaggact tgaaacaact tcctgttttt caatgtttcc ccctttagca acaagtaacg
    15061 tcccttgttt cccttgctct gtagctgttt ttattgtcat taaactaatc gaagcgattg
    15121 atggtagagc ttcaccacga aaacctaagg tccgaatacg aaaaaggtca ctctggcttt
    15181 tgattttgga tgtagcgtga cgtcgtaaac tgagaacggc atcctcacta gtcatccctt
    15241 caccgttatc cgttatttga atctttttaa gaccagattc ttcaacctct atggtaattt
    15301 ggctacttcc tgcatctatg gcattttcaa ctaattcttt aacgacgcta ctaggccttt
    15361 caacaacctc accagcagca atctggttag ctaatatatc cggtaattca ataattttag
    15421 acaagttcat ccctcctttt tttatgattt tttgtaactt tttttgtgat atcttagcca
    15481 ttataccata ttcaaaatat taatttgaca ataacacaat ttttagttga catcactcct
    15541 attacctgtt agacttaggg agcgtaataa gttttgaagt tgtatttttg tttaacattt
    15601 ttaccttttt tattttttac agtaacattt ttagaaaggt aataaacaaa tgacacaagg
    15661 tacagtgaaa tggtttaact cagaaaaagg ttttggcttt atttctagcg aaactggaac
    15721 agacgtcttc gctcattttt cagagattaa agttgatggc ttcaaaactc ttgaagaagg
    15781 acaaaaagta acatttgata ttcaagatgg tcaacgtgga ccacaagcaa caaatatcaa
    15841 tcttgttaaa taattaaaaa agagcctaaa tgaattattt agtctctttt ttgttgccta
    15901 aaggctattt tataataatt tctttaaatc aaatatagca ttcatagctt gcattggagt
    15961 tagattcatt atatctatcg cttctaactc ctcaataacc ctacctgcat ttgaatgttc
    16021 ttcaaaaagc gataattgtc cttgttgctt cccaggatct agttcctcat ttaagtcagc
    16081 agtaacagct tcttgggggg ctgatacgat caactgtact gcatcagctt ccaactgtga
    16141 taaaatatca gttgccctat ccaataagtc aattggtaaa ccagctattt ttgcgacgtg
    16201 tatcccataa gacttgtccg caggtccagg ttcaatttta tgtaagaagg taacttctcc
    16261 atctctctct aaagtagcca cgtgtacatt gacaagcctt gtcaattgtt cagataaatc
    16321 tgtcaactca tggtaatgag ttgcaaacat tgtttttgcc ctaacacggt catgaatata
    16381 ttctataatc gattgagcta atgccatacc atcataagtg gctgtccctc gacctaattc
    16441 atcaaaaaga atcaaagatt tatcactggc tcgttttaca gcttgattcg cttccatcat
    16501 ttctaccata aaggttgatt gcccagaaat taagtcatca gcagcaccaa tcctagtaaa
    16561 tattgcatca aatacaggca aatcaacttc gtctgccgat acaaaacctc ccatttgtgc
    16621 cataataact gtcagagcta actggcgcat ataggtcgac ttaccactca tatttggtcc
    16681 tgtaatcaac tggatatctg tctgactatc aaaatagata ctattgggaa tatattcttg
    16741 cactcccatc actttttcaa cggttgcatg acgcccattc ttaatcttaa tctgatgttc
    16801 atcattaaat ttgggacgaa catagtgatg attttctgca actactgcca aactctgtag
    16861 aacatcaacg gttgcaatag tctttgctaa ctcttgaaga cgtttaatat aagattctac
    16921 ttgagcacga acacgcataa aaatatcata ttctaaattt gaagattgct cgcgagcttc
    16981 gagcatttca ccttcaattt tggctagttc tgctgttcca tagcgttcag aattttttaa
    17041 tgtcgcttta cggaaaaaat gctccggtac tagtgataaa ttggaattgg tcacatggaa
    17101 gtaatagccg tcttttttat tataatcaat tttaagggta ccgataccac ttgctgctct
    17161 ttccttagct tcaatatcag caatccaacc tgtaccttca cgcatcactg ttcgataatt
    17221 atccaattgc ttatcaaatc cagacttgat aatgtttccc tcagtgatag tagcctgtgc
    17281 ttctggggct atcgccgtat taattaaact ttctaattca ggcatagtgt caattttgtt
    17341 gacaatgatg tcaagctcag gttgattgaa ggactgtaaa atcattttaa tccgaggaat
    17401 ttgtgataag gtctgtccga gttgcaatag atctttaggg ttggcttttc cgaaagatac
    17461 tcgacttgct aggcgttcaa tatcatatac accctttaaa ctttctgtga gatcgtttct
    17521 ctcaaaaaaa taatcaagaa acacttgaat aatatcttga cgttccttga ttcgattcat
    17581 acttaccaaa ggcctgtcaa tccaagttct cagcattcga gttcccatcg ctgttttagt
    17641 ctcatctaac aaccagtaaa gacttccgtg cttcttgctt gttctagcat tctccagtaa
    17701 atctagacta tttttcgttg catatgacat ttgtaaatag tcctttattt cataatgaac
    17761 tactttctgt aaatgactaa gttcacgctt ttgtgttcta tgcacatatt gtaaaagttt
    17821 ttccgcagca gatatttcca aatcagttaa ctgctcatca atcaaatgaa tgtcattaag
    17881 tcgttcgtct tcgtatgata aaagcaagtt catctgcttc gtcagtaggt gatttttttc
    17941 gtccgtcaat tgatatccta ctacaatctc acgagctttt aaatttagta tttcactacg
    18001 gacggactca aaatctgtta aaagggttgc ctgaaactct ccagttgaga catccatata
    18061 tgctagacca aatgtttgtt gatcttgcga atcaatcgct actaagaaat tattagcact
    18121 atccggcttc gttgactcca caaccgttcc tggggtaaca acttgcacta cctcacgctt
    18181 gaccactccc acagcttttt taggatcttc catctgctca gcaatggcta ctttgtaacc
    18241 taattcaact aaaacgtcaa tatactgttg agctgagtga tagggaactc ctgccattgg
    18301 gattggcttt tctgcgttct tatttcgact agtcaagcta atttccagga tttgtgctgc
    18361 ttttaccgca tcatcataaa ataattcata aaaatcaccc attctaaaaa gcaaaaaagc
    18421 atctggataa ttctctttta tatccagata ctgttgcatt cccggtgata tcgttggctt
    18481 ggccatttct cactcctaac taacggggct ggctgccatg ctcagctcct caatcattat
    18541 atttataaac ctactgatag ttctttttca aaaactttgg caatatcttc attttgacaa
    18601 atcaccaaca aggtgtctgc tcctgccatt gttcctaaaa tctctggcag ccctttctca
    18661 tcaataaagt ttgctaaaac gctagcctcc cctaaattag tatttaaaac taacataaaa
    18721 cttgctcgcg atactttcaa aatataggag cgtaaggctg ggctaaatcg atttgaagta
    18781 gctgttgata aactataata tagcttacct tctgggctac gcaattttaa aagaccaatc
    18841 tcacgcaagt ctcttgatag agtcgcttgc gtgacatcga ttccttcatt ttttaaatgt
    18901 aatttaattt cttcttgtgt gccaatctgc cctgattgga ttaaacgttt aatacgtttt
    18961 tggcgttcta ttttattcat gttattcaat cctttttagt tagaattatg cttaccaaat
    19021 tatatcaaaa aaaattgtct ttttcactta gtagtgatat cgtaatcatt ctgtaaaaaa
    19081 gctataaagt aggctagttc tttacagaat gcattatgaa tttaatcaac aagttctcgt
    19141 ctatttttca taattatgtt aaaatagggt gactatatta ctaattaaaa taggagatac
    19201 catggataca aaacatctaa ttgcgagtga aattcaaaaa gttgttccat atatggaaca
    19261 atcggccatt ctttctttat tagaaacccc aaaaaattcg agcatgggag atttagcttt
    19321 cccagcattc tctttggcta aaactctacg caaagcacct caaataattg ctagtgacat
    19381 tgctgaacaa attaaaagcg accaatttga aaaggtggaa gctgttggac cttacgttaa
    19441 ctttttcctt gataaagccg caatctcaag tcaagtttta aaacaagtct tatctgatgg
    19501 ttctgcctat gctactcaaa atattggtga aggacgaaat gttgccattg atatgtctag
    19561 tccaaatatt gctaagccat tctcaattgg acaccttcgt tcaacagtta ttggtgatag
    19621 tttagctaat atttttgata aaattggcta tcatcctgtt aaaattaatc accttggcga
    19681 ctggggtaaa caatttggaa tgttaatcgt tgcctataaa aaatggggca atgaagaggc
    19741 tgtccgtgct catcccattg atgaactttt aaaactttat gtccgtatta atgctgaagc
    19801 cgagatagac cctagcgttg atgaagaggc acgtgagtgg tttcgtaaac ttgaggctaa
    19861 cgatcctgaa gcaactgaat tatggcaatg gttccgtgac gagtcattat tagaattcaa
    19921 ccgtctttac gataaaatga atgttacatt tgatagctat aacggcgagg cattctacaa
    19981 cgacaaaatg gatgaagtgc ttgaactgct agaatctaaa aacctcctag tcgaatctaa
    20041 aggtgcacaa gttgttaacc tagaaaaata cggtattgaa catcctgccc tcattaaaaa
    20101 atccgatgga gcaactctct acattacacg tgacttagct gctgcacttt accgtaaacg
    20161 tacctacgat tttgcaaaat caatctacgt tgtaggaaac gaacagtctg cacactttaa
    20221 acaactaaaa gctgttctta aagaaatgga ctacgattgg tccgatgata tgactcatgt
    20281 tccttttggc cttgtaacaa aaggaggagc aaaattatca actcgtaaag gaaatgtaat
    20341 tctccttgaa cctactgttg cagaagctat taatcgtgcg gcatctcaga ttgaagctaa
    20401 aaaccctaac ctagcagata aagataaagt tgctcaagca gttggtgtag gtgctattaa
    20461 attttacgat ttgaaaactg atcgtacaaa tggttatgac tttgacttag aagcaatggt
    20521 atcatttgaa ggtgaaactg ggccatatgt acaatatgca catgctcgta ttcaatcaat
    20581 cttacgtaaa gctaacttta acccatctaa tagcgataat tacagtctca acgatgttga
    20641 aagctgggaa atcatcaaac tcatccaaga ttttcctcgt attattgtgc gtgcagcaga
    20701 taattttgag ccctctatta ttgcaaaatt cgctatcaat ttagctcagt gcttcaataa
    20761 atattacgcc cacacacgta ttttagatga agatgctgaa atcagtagtc gtctagcact
    20821 ttgctatgca actgctacag tattaaaaga atctcttcgc ctcctaggag ttgatgctcc
    20881 gaatgaaatg taagaaatga cattactaag actgtcaaat ttatgacagt cttaattttt
    20941 tatattataa taatagcaca ataagttaca ggagaatgcc atgccatcag aaaaagaaat
    21001 attagatgct cttagtaaag tttatagcga agaagtaata caagcagatg attattttcg
    21061 tcaagctatt ttcgagctcg ctagtcaact tgaaaaagag gggatgaact cactcctagc
    21121 tacaaaaatt gactcactta tcaatcaata cgtcttaaca catcaatttg atgctcctaa
    21181 aagcatattt gatcttagtc gcttggtaaa aacaaaagct agccattaca agggaactgc
    21241 tatctcagct attatgcttg gtagtttctt aagtggagga ccaaaataaa aatacaggcc
    21301 agacagcctg tatttttatt tatcgtaatt tcttcgtcga attttaaaat ctgagctaat
    21361 aacttcatct acatttataa cagaaataaa agcatctggg tctaaatcag ccatgatttc
    21421 tttcacatca cgcacttcac ttgggttaag agctacgtac aagacatcat gctctctacc
    21481 tgagtaggca ccttggcccc gtaaaaaagt tacaccccta ttaatttcat gcaaaatggt
    21541 tgctgctgca gattctgatt ctttggtaat aatgatcatt ccacgaacag tgtagccccc
    21601 attttcaaca tttgttaaca cttgactaaa tacaaagcta gctactaacg tatatagcat
    21661 ctgttgtaaa ttaatataaa caagtgaaga cgttagaact atagcatcaa taaacaataa
    21721 ggtttgccct aattttattc cagatttttc ctctacaata cgaccaataa tatctgcacc
    21781 acctgtagtt gcaccatatc taaatactaa accactacca gttcctgcta aaattccagc
    21841 agctactgca actaacatca tatcgttttt caaagggact gttacaggga tttgttgcca
    21901 aaaccacatg aaaaaagaca ttaacactgt accatatata gttaataata aggatttttt
    21961 ccctaaaatc ctagccccta atataaataa cgggatatta aggagaagtg atgaaagtgc
    22021 tggattaact ccgaaaagtg catggataat caaagtgaca ccggaaatac ctccttcagc
    22081 caaatgatta gccatattaa atttgacaaa gccgaaagta tagagagcca caccaaaggc
    22141 aattaaaata gcttctttta tctttgtttt taaatccagt ttcaacatag gcttaatcct
    22201 tccttattca aaaaagctaa agttgtttaa acaacttagc ttaataacat cataatgttt
    22261 ccttagtggc ggttgaccaa aagtcatata tacagttaat catcatcaac gaatcgtcca
    22321 ataatatgaa cattttctgc aacggaaaca aaggcttttg gatctgcttt tagcattaaa
    22381 tatttaaaat cactaaattc ttcacgtgtt aatatagtta ttaaaactgc ttttttctca
    22441 tgattataag taccttcagc atcattgata caggttacac cacgatgtaa atcacgatgg
    22501 atacgtttaa tgacgcaatc aggcttactg gtaacaatca tcgcttgcat ctttttttgc
    22561 tttgtaaaaa tggcatctgt aactctactt gatacaaaaa ttgtcaccat cgagtacaaa
    22621 gcgtacttcc aaccaaataa aagacctgca aataataaaa taataccatt aataataaaa
    22681 gaaatactac ctacatctct gcctgttttc tttctaattg tcaaactgat aatatctgtt
    22741 cccccactag aaattctaga cttaaagcta aaaccaacac cagctcccat aattaagcca
    22801 ccaaagatag cattgattaa gggatcagtt gtaagggtga tttgaggcat tagttgaatg
    22861 aagatagagc ttacagttac tgtgataaac gtaaaaatag taaatttatg tccaatctta
    22921 cgccaagata atatcagcaa agggatatta atggcataaa aagctaaagc aactggtatt
    22981 tcaaaagaaa accagtgttt actgacagct gaaataacct gagctaaacc agttgctcca
    23041 cttgaataaa catgtccagg ttgaaaaaag aaattaacag caacactgga tagaatacca
    23101 tacagtaatg aagccgaaat tttctctgca tatttttctc gtgagatact ttttaaagtg
    23161 tgtaagagac caaatttttt agcccataca ctgataatat attttacctt tttttctaaa
    23221 ggggttcgtc tagttttcat aactttctac agtaagtgat aattcttcta attgttgttc
    23281 tgaaacgaga ctaggagctt gagtcattgg atcactagcc ttattgtttt tagggaatgc
    23341 aataacttct cgaatattgt ccttgcctgc taacaacata acaaagcggt caaggcctat
    23401 tgccaatcca ccatgtggtg ggaagccgta atccatagct tcaagtaaga aaccaaactg
    23461 ttcctgagct gattcagcgc taaatcctaa agccttgaac atacgctctt gtgtatcttt
    23521 ttggttaata cgtaggctac caccgcctaa ctcatatcca ttcaaaacaa tatcataggc
    23581 aactgcacgt actttagcca aatccccttc caactcatgc gctgtctccg ctgttggcaa
    23641 ggtgaatgga tgatgagccg acatatatcg tccctcttct tctgaccact caaacattgg
    23701 ccaatcaaca acccatagga aattaaattt agaataatca atgagttcaa gttcttttgc
    23761 aatgcgtgta cgaagcgctc ctaaagtctc gttagctact tcaagactat ctgctacaaa
    23821 tagaattaag tcattattct ctagctgcaa agcttctgtc aacctacctt caatagctgt
    23881 caagaactta gcaaccggac ctgaaatagt attatctaca tattttagcc atgctaaacc
    23941 tttggcaccg tattgttttg caatttctgt taatttatca atattttttc gagagtactt
    24001 gtccgccttg tctttgacaa caatcgcttt aactactgaa gcttctgaga acaccttgaa
    24061 gtcaacttct tttacaattt ctgtcaagtc ttgtaaaagc atatcaaacc ttgtatcagg
    24121 cttatcagac ccgtagttat tcattgcatc atcatatgcc atccttggga atggtaaaga
    24181 aacctctaaa cctttagtgt ctttcattac tttagcaatc ataccctcaa caatgtcttg
    24241 aatttcttga tcactaagga atgatgtctc taagtcaact tgtgtaaact caggttgacg
    24301 atctccacgt aaatcctcat cacgaaaaca tttgacaatt tgataataac gatcaaatcc
    24361 tgcattcatc aatagctgtt ttgtaatttg agggctttgt ggaagggcat aaaaatgtcc
    24421 ttggttgaca cgtgatggaa ccaaatagtc tcgtgccccc tctggagttg attttgttag
    24481 catcggagtt tcgacatcaa taaactctaa gttgtcaagg taattacgga ttgagtgtgt
    24541 tactttcgca cgtaatttga aattttcaag catttccgga cgacgaagat ccaagtaacg
    24601 gtaacgcatg cgtgtatcat catttgcctc aataccatct ttaatttcaa atggtgttgt
    24661 tttcgaggta ttaagaaccg ataattcttg cacttttaat tcaacttctc ctgttggtaa
    24721 attatcatta gcttgttctc gtgctgtcac tacaccgcta acctctataa caaattcact
    24781 acggagactt tctgcggttg ccataactga ggctgctact tcttcaggat taataactaa
    24841 ttgcatgatt ccctcgcgat cgcgaagatc aataaaaata aggcctccaa ggtcacgacg
    24901 acgaccaacc catcctttta aagtaatcga tgttcctatg tgctcactac gaacacgccc
    24961 agcatacata ctacgtttca tctattttct ccatttaaaa tattcattcc aacctattat
    25021 aacaaaaagc acctaaaaac cccacccatt aaggagagtt tttaggaact gttttacaat
    25081 cctaattgtt taagcacaga tgaaaaatct gttttaatgt tttcaaaact aactttaact
    25141 tcttgacgtg tttgattatt ttttaaaccg acctctttcg aatctacttc actgctacct
    25201 aatgtcataa tcactttagc attaaaagta tctgcagact taaattgtgc tttgatttta
    25261 cgacccaaat aatcacgctc tactttaaaa ccttgatgac gtattgattg tgccaaatct
    25321 aaggcagcta gattagcctc tgatcctaat acagctatat acagatctat agtattttct
    25381 attggtaaag aaatcccttg cttatctaat attaagagca agcgttctaa tcctaatcca
    25441 aaaccaaatg ctggtgtttc tggtccacca aaataggata ctaagccgtc ataacggcct
    25501 ccagcacaga tcgttaattc attatcttca atctcagtga taaactcaaa aatagtatgg
    25561 ttataataat caagaccacg aaccatatta gtatcaatga tgtatggaat atttagagca
    25621 tcaagcatat gacacacagc atcaaagtgg gcttggcttg actcatctaa ataatctaaa
    25681 atagaaggag cattttcaac tgctagtttg tcttctggct ccttagagtc tagtacgcgc
    25741 aatggatttt cattcaaacg acgttgacta tccttagaca attgttcacg tatgggtgtt
    25801 aagtaatcaa taagagcttg gcgataggct tgacgacttt ctggacttcc taaactatta
    25861 agatgaagtt taacgttttt gatacctaaa tcttcaaata gatgatgtcc cattgcgatt
    25921 gtttcaacat cagttgttgg attatttgat ccaaagcact caacgcctac ttgatgaaat
    25981 tctcgtaaac gaccagcctg tgggcgctca tatcggaaca ttgatccaat ataatacatt
    26041 ttggttggtt tttgaacttc tggtgcaaat agtttatttt caacataaga acgtacaaca
    26101 ggtgctgttc cttctggacg aagcgtaatg tgacgatctc ctttatcatg aaaatcatac
    26161 atctcttttg taacgatatc cgttgtatcc ccaactgagc gactaataac ctcgtaatgt
    26221 tcaaacattg gtgtacgaat ttcatcataa tgatattgtt taaagagatt gcgtataaca
    26281 ttttcaacgt attgccactt tgctgattct ccaggtaaga tatcctgagt cccttttggt
    26341 ttttgaagtt tcataagttt acctcttcga ttagttttct ctttaaacat tctaccattt
    26401 tttcttatat tttaaacaag catatatcat ttgctttagt ttgatagtat tttcaccatg
    26461 aaattgtaaa aataaaaagt tttgtcaagt aactttactt tacaaaaaag atatgttatc
    26521 atagtgaagt tgatgaaaat caaaacttac acatcgaaag gaagaataag aaaatggcag
    26581 tacctgcacg tcacacttca aaagcgaaga aaaacaaacg tcgtacacac tacaaattga
    26641 cagctccatc tgtacaattc gacgaaacta ctggagatta ctcacgttca caccgtgtat
    26701 cactcaaagg atactacaaa ggacgtaaaa tcgctaaagc taacgaagct aaataataga
    26761 agggagatac catgcgcgta aatattacac ttgaacataa agaatctggt gaacgcttgt
    26821 accttacttc aaaaaacaaa cgtaacactc cagaccgtct tcaattgaaa aaatactcac
    26881 caaaacttcg taaacacgtt gttttcactg aagttaagta agggtttcag tacacgtcaa
    26941 tagacgtcaa aatattgatt ttaagcgatt tttgattttt tgaaactcat tatatcgcac
    27001 tacaaatcaa taaaaactct accttttact ctaccttttt tagataaata tatcatctgg
    27061 atattgaagt taagccctgc tatcatttcg atggcagggc tttttaatat ttgtacacta
    27121 acttcttatc tatattttgg tggattttaa gttgacatct acaaagttta atgttagaat
    27181 acattcaaaa atatatttga atgaggtatt ttatgattca aaatgttgtt acttcaataa
    27241 tcctgtattc tgggacagcc gtagacttac ttattatcct aatgttattt tttgccaaaa
    27301 gaaaaagcag aaaagacatc attaacatct atttaggaca atttctaggc tctgttagtc
    27361 taatattcct aagtttgctt tttgcatttg tcttaaatta tattcctagt aaagagattt
    27421 taggtttact cggtttgatt ccaattttcc taggcctaaa agttttgctt ttaggagatt
    27481 ctgatggaga agctattgca aaagatggtt tgcgaaaaga caataaaaac ctgatttttc
    27541 tagtcgctat gattactttt gcaagttgtg gcgctgacaa tattggtgtc tttgtcccat
    27601 attttaccac cttaaattta gcgaatttga tagtgacttt acttactttt ctagtcatga
    27661 tttatctctt ggttttttct gcacaaaaat tagcacaagt cccttctgtt ggagaaactt
    27721 tggaaaaata tagcagatgg tttattgccg ttgtctattt aggattgggg atgtatatcc
    27781 tgattgaaaa caacagcttt gacatgctat gggctgtgtt aggctaggag aaaaaattat
    27841 gaaaaaagat agtatctgtc aagtggatgt tataaatcaa caaaatgtta caaccgcaac
    27901 gaactacctt gaaaaggaaa aagtccaaaa atcacttcgc attttatcaa aatttaccga
    27961 taataaacag ataaatatca tcttttatct ccttgccgtc gaagaactct gtgtctgcga
    28021 tatagcctgt ttattaaatc tcagtatggc atctgcctcc caccatcttc gtaaactagc
    28081 caatcaaaac atcttggaca ctagaagaga ggggaaaatt atatattatt ttataaaaga
    28141 tgaggaaatc agagattttt ttaatcaact aggataacaa ctatttctac tactttctac
    28201 ttttccatga ttataggggg attatatatg aaattttttg aagaaaatta ttcacaagaa
    28261 atacctactc gtatcaaaaa tttaagaaaa aaatacaata ttacacaaag cgaactaggc
    28321 aatgcaggtc aagttagtca agttgaaagt ggtaagcgac caattacgag ttcgatgtta
    28381 gtttatttaa atgctctaac cgcttcgagc tatacgtata tcgtttttgg agagttagat
    28441 gagtttattg agaatctgtt tcattatttt ttcagttcta ttttatatag agatttagag
    28501 gctgtagatg aaaaactata ttctttcatg agtgatgatt taatttcaat ccaatcaagt
    28561 tgtctaagta ttgccaagac atttgcaaat ttcaatattc aaagaaaaag atttatgatt
    28621 tctactgaaa ctgaaatgga tacttttcac aagaaagatg acattgacgt atgggttggt
    28681 ggaaaaagtt ataaccccgc tagaagtttt agaacccgta ccatcaatga attgactgtc
    28741 attgattttg aagaaatgtt tgatattctt tggttgatgt taggggataa tttaattaaa
    28801 tcatttgaag ttaatgtctg tggtatccta tttgaattag gcggaaatga cataccctca
    28861 acatttaggc aagaaaacat tgatcctctc atcaataaat ggtggtatga taatgtttcg
    28921 acagaaatta tccctaatct aataaaaaaa ctcaaagaaa atcccttgtt taatatcgga
    28981 tttatggtaa atgatatatt agaaagaatg tataaagaga atattccaaa atcttacctt
    29041 acatccgtcc cattagttat ttctcaaaaa ggaagaacaa cctattcgtt tagtatgact
    29101 ggtggtcaac aaatagatgg agtgaaattc acacagatat atgaggacta tatgaaatta
    29161 ctcagtcaag gtaaggatat cgcagagtta tatcaaaaat attctaaaga agagttggca
    29221 aatctaggca ttaatattta tcaatccaat gatatagaaa ggactgagga aagaactttt
    29281 gatgaaatta tcagttgggt ttccaaccct tatgcaacaa gaccaattca agaaaggcac
    29341 actattcaat tagagccaac aagattttca ctagaggata agaaaagaat tgaagaagct
    29401 gcagctcaag gactaagcga aatcgacctt attgatttag ttgacctata tgatattaat
    29461 ttagacaata caagcgtcaa tcgccatatt gtggggttat tgactaataa cacccaagta
    29521 acatactatt tccaagaaca attaaataag gagttgctgt caatggctca cgctttagat
    29581 aacgtacaac aggcctttat taaattatta agtgaagagg agatacgaaa atttgctctt
    29641 taaagactag gtgaatcgcc tagtcttttt tgcgcctcgt tttacaaatt agtctattga
    29701 agtaaaaaaa gaagtaaaaa ataaaagaac attacgcctt atactgaata tatcaagaaa
    29761 aactcttgat ataaatttta atagtccaaa ttgatggcca tcaaaggaca gattgaaaca
    29821 aatatgatga catgacgata aaggtcatgt cttgcataga attgtttcgc tgtttctttg
    29881 ttgtgccttt tggtatactc agcgttcaat atttgctcat tttgtttcct tctgtccaaa
    29941 ggacagaaag gagctcatca ttatgagtaa taaagtacaa gaacgtcgtg agcgtaaaat
    30001 caaagaagct attaaagcta aaaattggga tgaagtaact cgtttgcttc aacaagaaca
    30061 aagtaacgca gaacgtcgtg accgatacca caatagacga atcaaagacg aaacaatagc
    30121 tagcaaaaat gccaaaaagt ctgtacgtta tgatgtcatt gcaagttctg atttgaatcc
    30181 cgaagaagct ttaatcctag aggagttaag acaagctatt cgtgaagcta aagcatccct
    30241 atcagaaatt gacagtaaga ttgttgaaat gatagcggaa caaggttcaa gctataaaga
    30301 aacggctcgc tatatcactg aacattataa gaaaatgagt gatgtaactg ttaaatccca
    30361 ttattgtaaa gcgctaaaaa aattagctcc tttattaaaa gcttaccgct aatccctcaa
    30421 atccagtgtc tctgtcactg gattttttgt aaaaaaaaat taaaaattca acaaataact
    30481 ttgcgtttag ctgtctcatt ctccttagta atgaggaggt ggtcagataa cctaaaaaac
    30541 aaagtccagc tcatgaccca aaccgagcct gtcgcattca cactatctct tattcataag
    30601 gaggatttgc cctatggcgt ttaaaatgaa aaccacaaaa ggtggttata caactacatt
    30661 ggctgataaa attatcagtc aaaaacaacc gatttactca cttagtaccg aacttgaacc
    30721 acaacaacgg tttgaagaag gaaaaccaac cggagaaatc gtagcttata aagcatggtt
    30781 tgtacaggaa gggcaagacc ctttccaagt aaaatttgaa gaaacgattg agcttccagc
    30841 ttttcaatcc atgattcaat ttgacacact acaagcctgc gaagtaaaat ataacatcta
    30901 cttcaaagca aatggtatca aggaggttcg gtaatgactt cacaatctcg tgatgtgctg
    30961 aaccaatctt acctgcaatc ttacctgctc cagagcctaa acatggctct gggggcttta
    31021 atgcagggag aaaccagcta caccaattct tttaatattg ttattcaagc agacggcttt
    31081 atctttgtcc ctcgtctacc atgtgcttat atccttgatg atgacttgta caagaaaatc
    31141 tttctgattg ccaatgcttc actttatcca caatatacgc ttctaaaaca aaacgctacc
    31201 tattttgtcc ctctagaaac agatgactta cacactcagc gtggattatt tttcccttgg
    31261 aaaaagggaa tttcagaacg tttagtcatt ccagatttgg ataagttttc agctagcttg
    31321 ccacagggga aaatccctat catgaagtac tttgaactca atttagataa ggttaatcac
    31381 tgggctattg ctggaaattc aggttctggg aaatcgtacg ctctcactta tttcttgagt
    31441 gtactaaagc atatgtctga cttgattatc attgacccta aatttgatac cccaagtcga
    31501 tgggcaagag aaaatcacgt tgctgttata caccctgttg aaaaccgttc aaaatcagat
    31561 tttgtctcac aggtcaatga acaattaagt cagtgtgcaa ctctcattca gaaacgacaa
    31621 gctatcttgt atgataatcc caatcatcaa tttacccatc taactattgt tattgatgaa
    31681 gtcctcgctc tatcagaggg agtcaataag aatatcaaag aagccttttt ctccctactc
    31741 tcacagattt ccttattagg acgtgctacc aaaatccatc tgtttttggt aagccagcgt
    31801 tttgaccaca atactattcc aatttcagtg agggaacagc taaacgtatt gttgcaaatt
    31861 ggaaatatca atcagaaaac tactcaattt ttatttccag acctagaccc agagggtatt
    31921 gttattccaa taggacatgg gacaggtatc attcaagtta tcgataatga acatccctac
    31981 caagttctgc ccttactctg tccaacctac tacactaaac gaggtattct atgaccaata
    32041 aaacctactt tatgagaagt tttaatctca tactatattt atttctattt gcacctatct
    32101 gttctgcatt tggatggtta ttcaattatc tttccgttca attaacaaat atcacgtttg
    32161 tcaatttaga tacggtcaaa agtattacgg atatcattag ttccgtttgt catggatttg
    32221 cacttatttc tcttctactt gccttatttt tggttggatt agaaataata caacggttga
    32281 aaacagatag tctttggaac tatatccaat ctgtctatca cacttttctg ttgcgacact
    32341 tcctgtttca gcgtgaacga gtacagaaga taagcaatct tgaacatcag acggttacta
    32401 ctatcaaccc aatccataac ggatttaacc gagctgttcg taagtgtatc gtagatatac
    32461 gaaaagatac catcactatt tttctaaaag tccctagaga ccaacaaggg caaaaaatcc
    32521 taaaagagat ggaagcacaa ttaaaagaag aaatcgtcag ccaacacgca gactattatt
    32581 tctcctctcc tattcgagtt cgtaaccaat tgtggttcac tggacaaaaa cgttaatccc
    32641 ttggggctgt ggcttgcgtt aagcaaaagc cagacagccc ctttttatat tcttttctat
    32701 catgacctat tggggtgact acgaccccca ataggtcaat aatcaataat cacaaaaaat
    32761 tcaatttttt caaaaaaact ttgcatacga cctgtcattt ctccttagta atagaaccca
    32821 aaagaaatga ggtaactgcc tatggcaaaa gaacagcgct caaccaaatg gacttttctc
    32881 ttctacgagg aaagcgcccc agagaactac ttgaatatat tagaggaact ccatattccg
    32941 tttattctta gtccttggca cgataaggac gttaatagac agacgggaga gttcaagaaa
    33001 tcgcacaaac acggtgcttt cttctttgat tcactgaaaa gctattcaca agtatcaaat
    33061 atcatcagtg acaaactaaa cggtccagca catgtggagg tagttatgtc tcccactggt
    33121 ttatttgact actttaccca tgcggaaaat ccagataaga ccccctacaa cattgaggat
    33181 attgaagtcg gttgcggctt taatttagaa aaattcttga tggagatgaa ctcctcggat
    33241 tttatacatg aagtagttga tattatcgaa gagaacgact ttaccgaatt tgaagaattg
    33301 gtctggtatg cccgagcaaa caataccaat ttattagggc ttatcattga acgtacatat
    33361 ttcttcgcta aatacctaga ttcacgaaga tacaatccta atcgactcca taattcaaac
    33421 acagaggaga aagagaataa tgaataacga aaaattagaa caaattgaac aattattgca
    33481 aacacttatc aaactcattc aaatcaaaga acagaacata ccactaaaga taatacaaca
    33541 gcgagaattg ctaaaacagc taaatatctc cccaaacaca ctaaaaactt gggaacaaaa
    33601 aggattaaaa agattagaac ctcctataga gggaactcga accgtcttct atctactaga
    33661 tgatattatc aactttttac aatcctaatt ctgaaaattt gtgctaaaat agttttatct
    33721 aaaagaaaga gtggtaacta ttttagccta cttcaatatc catgaagaaa ggcaaatatg
    33781 gaaactgaaa caattattca taatggcaaa aaagtcatta aaaaaatcaa aaaagataag
    33841 tccatctcat acactttgaa ggggacattt ctgggaaaag atatcaaaac tggtaagcaa
    33901 gtcactacta ccattacagc caagacacta aaacagttag atagagcaat cattcaagca
    33961 cgactagaat ttgaaaaaaa tggctcaaca cgagaaaaag ttatcgtcat tgatacactg
    34021 gaagatttag ctgaagaatg gtttaaatca tataaaacct gggttaactc gcataatact
    34081 ctgaacagag taagaggtta tcttgataat tatattatcc ctaaatttgg agattacaaa
    34141 ccagataaaa ttgaatctgc ggatatccag ctttggttaa acgatttggc aaagaaatca
    34201 aaagagtcca ttgaatctgg tataaagcgt gctgataaag gtagtgctaa agattttggt
    34261 gctgttatcc ataagttgaa agatattttt gactatggaa ttacaaacta tggactaatc
    34321 actaatcctg ttagcactgt taagatacca cctaagccaa aatcaaataa gcaacgcatc
    34381 atggtattac atgatgatgg tcttgtaata tggcttaatt atcttgaaag tttagataac
    34441 aatagagcca atcgtagatt taagttaatt tgtaatactc tattagcctc cgctttgcga
    34501 ataaatgaat tacttgcatt gacgattgat gacctagatt ttgaaaattc ttctattaac
    34561 gtcagtaaaa cattgatgtg gaaaaccgca aataaaaaag atgggacaaa aggggaagtt
    34621 atctgtaaag ctacaccaaa aactgatgca ggaaatcgct ctgtccctgt ccctcccccc
    34681 ataatagaaa aactcagaga tttccaccac gaaatgaaca agtatcttga attgcacaac
    34741 agacatagaa caaaactgat tttccctaca atctatggga actatatgtg tgacagaaac
    34801 gagagaacaa ctcttaagaa aaggttagta ggtcttggat taccaaacta tggtttccat
    34861 ttattccgac atactcatgc ttcaatgcta cttaatgctg gaacaaattg gaaagaactc
    34921 caagtcagaa tgggacataa atccatttca actacaatgg atacttacgc tgaattagca
    34981 ccacaacgaa agttagaagc tgtggggatt tatcttgaaa agattgctga gctaacgcaa
    35041 taactactct accattcact ctacctttta ttatgtagca ttctagaaac attgatgtac
    35101 caacgtttct aacagctaaa atagaaatta tttaactctt actgaagtta aataattgat
    35161 tataaaaaag cctatgaaat caatatttct gggcttttta tttttaaaaa taaaatctga
    35221 caaaaaagta taaatttacc tcttaccttc aggtagttag tcattctttt aaaactcact
    35281 tttttataac tgaaaatatt gttcttaatt ttaaaaa
//
LOCUS       DATRME010000012        33276 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRME010000012 DATRME010000000
VERSION     DATRME010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33276
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            42..2150
                     /locus_tag="VBC23_000615"
     CDS             42..2150
                     /locus_tag="VBC23_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6842585.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAEQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLM
                     LDEVNQTIGKKGIDLVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     gene            complement(2243..3187)
                     /gene="mmuM"
                     /locus_tag="VBC23_000616"
     CDS             complement(2243..3187)
                     /gene="mmuM"
                     /locus_tag="VBC23_000616"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6842586.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(3192..4568)
                     /locus_tag="VBC23_000617"
     CDS             complement(3192..4568)
                     /locus_tag="VBC23_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6842587.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            complement(4698..5348)
                     /locus_tag="VBC23_000618"
     CDS             complement(4698..5348)
                     /locus_tag="VBC23_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6842588.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            complement(5360..6009)
                     /locus_tag="VBC23_000619"
                     /pseudo
     CDS             complement(5360..6009)
                     /locus_tag="VBC23_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            6101..6649
                     /locus_tag="VBC23_000620"
     CDS             6101..6649
                     /locus_tag="VBC23_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6842589.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            complement(6711..7307)
                     /locus_tag="VBC23_000621"
     CDS             complement(6711..7307)
                     /locus_tag="VBC23_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6842590.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(7318..8544)
                     /gene="clpX"
                     /locus_tag="VBC23_000622"
     CDS             complement(7318..8544)
                     /gene="clpX"
                     /locus_tag="VBC23_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6842591.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(8762..9256)
                     /locus_tag="VBC23_000623"
     CDS             complement(8762..9256)
                     /locus_tag="VBC23_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6842592.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(9336..10175)
                     /locus_tag="VBC23_000624"
     CDS             complement(9336..10175)
                     /locus_tag="VBC23_000624"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6842593.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            10224..11501
                     /locus_tag="VBC23_000625"
     CDS             10224..11501
                     /locus_tag="VBC23_000625"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6842594.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            11503..12786
                     /locus_tag="VBC23_000626"
     CDS             11503..12786
                     /locus_tag="VBC23_000626"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6842595.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            12878..13327
                     /locus_tag="VBC23_000627"
     CDS             12878..13327
                     /locus_tag="VBC23_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO6842596.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            13320..13976
                     /locus_tag="VBC23_000628"
     CDS             13320..13976
                     /locus_tag="VBC23_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6842597.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            14063..14977
                     /locus_tag="VBC23_000629"
     CDS             14063..14977
                     /locus_tag="VBC23_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6842598.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            15078..15932
                     /locus_tag="VBC23_000630"
     CDS             15078..15932
                     /locus_tag="VBC23_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6842599.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(15978..16196)
                     /locus_tag="VBC23_000631"
     CDS             complement(15978..16196)
                     /locus_tag="VBC23_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6842600.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(16264..17259)
                     /locus_tag="VBC23_000632"
     CDS             complement(16264..17259)
                     /locus_tag="VBC23_000632"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6842601.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(17256..18248)
                     /locus_tag="VBC23_000633"
     CDS             complement(17256..18248)
                     /locus_tag="VBC23_000633"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6842602.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(18241..19185)
                     /gene="mvaD"
                     /locus_tag="VBC23_000634"
     CDS             complement(18241..19185)
                     /gene="mvaD"
                     /locus_tag="VBC23_000634"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6842603.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(19167..20045)
                     /gene="mvk"
                     /locus_tag="VBC23_000635"
     CDS             complement(19167..20045)
                     /gene="mvk"
                     /locus_tag="VBC23_000635"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6842604.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(20161..21390)
                     /locus_tag="VBC23_000636"
     CDS             complement(20161..21390)
                     /locus_tag="VBC23_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6842605.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(21390..22076)
                     /locus_tag="VBC23_000637"
     CDS             complement(21390..22076)
                     /locus_tag="VBC23_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842606.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(22078..22704)
                     /locus_tag="VBC23_000638"
     CDS             complement(22078..22704)
                     /locus_tag="VBC23_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6842607.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(22812..24566)
                     /locus_tag="VBC23_000639"
     CDS             complement(22812..24566)
                     /locus_tag="VBC23_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842608.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(24556..26373)
                     /locus_tag="VBC23_000640"
     CDS             complement(24556..26373)
                     /locus_tag="VBC23_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842609.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGLTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(26420..26860)
                     /locus_tag="VBC23_000641"
     CDS             complement(26420..26860)
                     /locus_tag="VBC23_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6842610.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            complement(27087..29915)
                     /gene="uvrA"
                     /locus_tag="VBC23_000642"
     CDS             complement(27087..29915)
                     /gene="uvrA"
                     /locus_tag="VBC23_000642"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6842611.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            complement(30047..30718)
                     /locus_tag="VBC23_000643"
     CDS             complement(30047..30718)
                     /locus_tag="VBC23_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6842612.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            complement(30743..31687)
                     /locus_tag="VBC23_000644"
     CDS             complement(30743..31687)
                     /locus_tag="VBC23_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6842613.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            complement(31857..32096)
                     /locus_tag="VBC23_000645"
     CDS             complement(31857..32096)
                     /locus_tag="VBC23_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6842614.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(32141..32632)
                     /locus_tag="VBC23_000646"
     CDS             complement(32141..32632)
                     /locus_tag="VBC23_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6842615.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            complement(32644..32931)
                     /locus_tag="VBC23_000647"
     CDS             complement(32644..32931)
                     /locus_tag="VBC23_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6842616.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
BASE COUNT        10763 a         6623 c         5507 g        10383 t
ORIGIN      
        1 attaactttc aaaaattaaa atcaattgag gtatttaata tatggcaaat aataattttt
       61 ataatcgtga cccttttgga aatatggatg atatttttaa ttctctaatg ggaaatatgg
      121 ggggctataa cagtgagaat aagcgatact taatcaatgg acgggaagtt actcctgaag
      181 aattcagtca atatcgtcaa actggaaaac tccccggtca agagctaaat aaccaaaaca
      241 ctcctacaaa ccaagtctct gcagatagtg ttttgactaa attaggaact aatttaaccg
      301 atcaagcacg tcagcatttg ctagatccag ttattggacg aaacaaagaa attcaggaaa
      361 ctgcagaaat tctagctcgt cgtactaaaa ataatcctgt tctggttggt gatgcaggtg
      421 ttggtaaaac agctgttatt gaaggtttgg cacaagcaat tataaatggt gatgtccctg
      481 ctgctatcaa aaataaagaa atcatttcga tagatatttc tagccttgaa gctggcaccc
      541 aatatagggg tagttttgaa gaaaatattc aaaatatcat aaaagaagtt aaggaaacag
      601 gaaatattat tctcttcttt gatgaaatac accaaatatt aggagccggt tcaactggtg
      661 gtgatagtgg ctctaagggg ctcgcagata tacttaagcc tgcactttca cgtggtgagt
      721 taacggttat aggtgcaact actcaagatg aatatcgtaa caccattctc aagaatgctg
      781 ccttagcacg tcgctttaac gaagttaaag tcaatgcacc ttctgcacaa gatactttca
      841 atatccttat ggggataaga aacctctatg agcaacatca taatgttgtt ttacctgact
      901 ctgttttgaa agcagctgta gatttatcta ttcaatacat cccacaaaga agtctccctg
      961 ataaagcaat tgaccttatt gatatgactg ctgcacacct agctgcgcaa catcccgtaa
     1021 ctgaccttaa atccttagaa aaagaaatcg ctgagcaaag agacaagcaa gaaaaagctg
     1081 tcaatactga agattttgaa gaagctctta aggtcaagac acgcattgaa gagttacaaa
     1141 accagattga taatcatacc gaaggacaaa aagtcactgc aactatcaac gatatcgcta
     1201 tgtcaattga gcgtctaact ggagttcctg tatcaaatat gggtgctagt gatattgaac
     1261 gcttaaaaga acttggcaat cgtctaaaag gtaaagtcat tggtcaaaat gatgctgttg
     1321 aggcagttgc gagagctatt cgccgaaatc gtgctggttt cgatgatggt aatcgtccga
     1381 ttggtagctt tctatttgtt ggaccaactg gagttggtaa aaccgagcta gcaaagcaat
     1441 tagcttttga tatgttcgga tcaaaggatg ctatcgttag acttgatatg tctgaatata
     1501 atgatcgtac agcagtttct aagctaattg gagcgactgc tggttacgtt ggttatgacg
     1561 acaatagtaa tacactaaca gaacgtatcc gtcgaaatcc ttattctatt gttcttcttg
     1621 atgaaatcga aaaagctgac ccgcaagtta tcacgcttct ccttcaagtt ctagatgatg
     1681 gccgtttaac tgatggtcaa ggaaacacta ttaactttaa aaatactgtt atcattgcaa
     1741 cctcaaatgc aggttttgga aacgaagcat ttacaggtga cagcgataaa gacttgaaaa
     1801 ttatggaacg aatttctcca tatttccgtc cagaatttct aaatcgtttc aatggtgtta
     1861 ttgaattctc tcacctaagc aaagatgact taagcgaaat tgtagatttg atgcttgatg
     1921 aagttaacca aacaattggc aaaaaaggaa ttgaccttgt ggtagatgaa aatgttaaat
     1981 cacacttaat tgaactgggt tatgacgaag caatgggagt acgtccattg cgccgtgtca
     2041 tcgagcaaga aattcgagat cgcatcacag actactatct cgatcataca gacgttaaac
     2101 acctaaaagc taatttgcaa gatggccaaa tcgtcatttc tgaaagataa aaacaaaacc
     2161 cgtttggaag aatccaagcg ggttttctag tggattgaaa aaggaaatca aacttaccct
     2221 aaccttcttc aagatacttt acttatttta aatgtgcaga gaggtcagca atatcagctg
     2281 gtctagttcg gcaacaacct ccgacaactt gggcgccaag tttttgccag tcacttgtat
     2341 tttcaagtag ggtgtgtgaa tgatcagggc tagattgcca agattggctt gccccatcat
     2401 aaacctctcc agagtttgga taggtcacaa gaggcttgtt agtctgctct gctattgctt
     2461 gcaagaaatc agcaactaac gatggacttg aacagttaat gcccagcgcc acgacctgtg
     2521 ggctaacatc aatagctttc gccaagtctg caacagcact accatctgag atggtcttac
     2581 catcttgaga ggtgaatgac atataagcct caacctgagg gaaatcctcg gcaagtagct
     2641 caataagagc ctcagcctcc tgagcgtttg gaatggtttc aagtgccaga atgtcaacac
     2701 cctcatctaa aagaagctca atacgatggc gatggaagtt tttaagacct tgcttatcaa
     2761 tatcgtagag tccagtgtac tcagagccat ccgctaaaaa ggcagcgtag ggaccaacat
     2821 ccccagaaat caaaggatag attctctctg atttttcttc ttttgtcaaa cttttccaaa
     2881 cttgctcacg cgcagctttt gccaactgaa cagtcaagcg aatcaaatct tctgcctgac
     2941 tttcagagac accaacttga gctagtcctt gtaaagtcgc ctgataagtt gatgtcgtca
     3001 caatatcagc gccagcacga atataatctt catgaatggt ctgaatagct gctggatcct
     3061 caatcagata tttggctgac caaagtttcc cagaaacatc acaacctcta ctctcaagct
     3121 cggtccccaa ggcaccatgt aggatgagag tttttttact ctctaaaagt tctttaaatc
     3181 ttcccataag atcaccattt cttgtaacgt agtttgtgat aaatatagca gaaaataata
     3241 aatggaacac cgaagtaaag cccagcacgt tgtgagctat cccaagcaat ccctacaatc
     3301 gaaatcaaga gcaaaatgat tgtgatataa gggagaactg gtgtaaaagg tgttttgtaa
     3361 gacaaatctt ctagttgatt ctctttcaag aattccttgc ggaaattgat ttgtgcgact
     3421 ggaatggcta gccatacgac aacaacagca aagcccgcga ttgaaaccaa ggctagataa
     3481 actgtgtctg cagcgtaaat acttgaaaag agcgacagca ctgctcctgc cattgacaag
     3541 agaagagcac gcattgggac accgtgttta ttgattttca caacagattt tgacaacata
     3601 ccttcattgg caagggacca gagcatacgg cttgatgcgt agagacctga gttaccagca
     3661 gacaggatgg ctgtcaagat aacgaagttc atgatatccg ccgtaaaagg gattcccatc
     3721 ttgtcaaaga catcaacgaa tggtgctgtg gatacgcctg cctctttcat tggcaatagc
     3781 gaagctagga caacaattgt cagtacaaag aaaacaacca agcgaccgat tgtcgtttta
     3841 atagcccttg gtacagcttc tttgggatta tccgtttcac cagccgcaat accaattaac
     3901 tcagtaccag agaaggcata gttaacagcc aacatgactg agacaactgc aacgatacca
     3961 tttggaaagg caccattggc agtcagatga gtgaagagaa tagccttgtg ctgaccttca
     4021 aaggaaacta gaccaaacat agcacctaag cccaagataa taaagataat gatagcaata
     4081 accttaatac ttgagaagaa agactctgct tctgcaaaaa agcgtacgct aagagcattt
     4141 aaaccaaaaa tcacaagggc aaaaaaggaa gcaaatgccc aagccggcac atttgggaac
     4201 cagcgctgca tcagcatggc agcacctaaa aattcagtcc ccaaggcgac cgtccaacaa
     4261 atccaatata gccaagcaac agtaaaacct gttccaggac tgataaactt agtggcataa
     4321 gtgtggaatg accccgtcac cggcatggca accgccaatt ccccaagtga tagcatgacc
     4381 aaataaacca caacggcacc aatcaaataa gacagcacag ctccaagcgg accagcctgt
     4441 gcaatggtat aacctgaact caagaaaagc ccagtcccga taacaccacc tagcgataac
     4501 ataaagagat gacgactggt catcttacgc tggaatttac cttcatcttc ataacgatga
     4561 ttttccataa caacctccaa gaaactaata cttttatcct accacaatct agtcgtattg
     4621 gggaataggt attagctatc atatatgata aagaaaaact attacaaaaa atcagcgaat
     4681 cataaatagc ggtaacctta tttttttatc cttacaaaat taacgacctt atgaaaacgg
     4741 tccatttgcc aaggactaat aatataattg gtctccttat ccttcaatag ctgtacaggt
     4801 gcaataatgc tagttagtac acagacaatc aacatgataa aaccacctag ccaatctccc
     4861 caagtgagca ttccttctgc actagcttga tagatgtatg cgcaagctat tggtactagg
     4921 agaaatactg tcgttgctag accaggatta taccaagttt taagtttaat attcatctga
     4981 aaaccatgac ccaagagctg cgttagacta aagaacatga ctcctaatcc aagccaataa
     5041 gcttgaggaa aagcaatact agcaatgtaa agcaaccaag caatagtatt aaccaacata
     5101 atagactgag tattgcctgg aaaacaatcc atcagctctt cttcatcata aacaacatag
     5161 ttaatgatag gtgatgcccc accgggaaac tgatattctt caaattgatg cgctagaagt
     5221 gccataaagt aaaaatacaa tagatagtga agcattgacc agtcaggtct aaaaacaagc
     5281 aatattactg ctaaaataat aaataaaatt gctccaaaac gataccagta atttctaaaa
     5341 tatgacatga taatacctcc tatttaaact gtgtttcacg aaaatgtgtt aaaaatggtt
     5401 ttgagcgtga aagatcaaca tcactaaatg cgtaatttga atgtcgacct aaacgcttat
     5461 agattgggga acgaaaggct ataaagtagt taagaacaat ccatatcagc ctaaaaacca
     5521 atcaagtcca gaatacaact tccaatcatg agctaaataa tagacagaaa cagggacaag
     5581 acctactaag gtcgtaagaa gtcctggatt ataccacttc ttcaaggaaa gattaaaaaa
     5641 gaaaagatgc atggcaagtt ctgcaaatgc aaaaatcatg actgcaagtg ttaaaaatgg
     5701 tatattggga caaaaaatcg gcaaaagata aacaatcact gcaaaccact gattcccgaa
     5761 aaaggctgat agatggttta agtcccactt actagtatca gtttcaaccg atttaagttc
     5821 aactagcata gtgataagcg gaaaacctcc agggaaacca aattcttcga aaaagtgtaa
     5881 ttgaataaaa agtcccgaaa taagtagtga ctgcactcgt aaatcccaat ttccaaaaaa
     5941 taatgctaag gcaaaaagag aagcaagata gggacaaaag aggtaccatt ttgttatcaa
     6001 tgctttcata agatgacctc caagatttgt gacatttgat accatatagt ataatcaagt
     6061 gaacctataa tatcaatacc tttggaggtt ttatgcacaa atgactgaaa gtgtgacagt
     6121 taataaaaag agaataaaac aaatcactca aacacaagag gatatcttaa cagcttttgg
     6181 ccaacttttg gaagagtatc cctatgagaa aattcagatt agccaaattg ccaagaaatc
     6241 tggctatgct agaaggacct tttaccgaca ctttgacagt cgagatgacc tacttaccct
     6301 tttcatcgaa agactaacat taaatctctt taagcaactt ggtcaattgg agcaacctac
     6361 ttttagccaa gttttccaaa atttttttag ttattggtct gattataaat cccttcttct
     6421 cattcttaga aagaatgacc tactccctca atttcaacaa tcctggttcc gacatattga
     6481 tttgatagag ttaggtcgtg gtgatttatc ttccaatacc tatgctcagc gatttgcgat
     6541 tggtggtatt ttcagtgttc taattgagtg gattcatcag gactgccaaa caagtatcga
     6601 agaattaact caagtttcct tcgatattat caatcattta aaaaattaaa aagagctttg
     6661 cagtagtctc aacagctact gcaaagctct ttttgtcata aactctcaaa ttataactca
     6721 gataaaatag tatcccatga ttcatcaaga cctgtgcggt caacagatga aaagacgatg
     6781 aaatggtcct ttttatcaaa gtttaatttc ttcttaatag acgactcatg tttattccac
     6841 ttgccacgag gtattttatc tgcctttgtc gctacaataa ttactggaat ctcatagtac
     6901 ttaagaaatt catacatttg aatatcgtcc gcacttggat catgacgaaa atcaactaaa
     6961 ctaacaacaa cacgaaggtt atcacgtgtt acaaggtact cttcaatcat cttgccccac
     7021 ttagcacgct cagttttaga caccttagcg tagccatatc cagggacatc gacaaaacgc
     7081 agtttgtcat caatgttata aaagttgagc agttgtgtct tacctggctt actcgacgta
     7141 cgcgctaggt ttttacgacc aagaagcgtg ttgataaagc ttgatttacc aacatttgaa
     7201 cgaccagcga gagcaacttc tgggagatca tcctgaggat aatgtgactt gttagctgca
     7261 ctcaataaaa tagacgcatt atgagtattt aaaaattctt ctgccatctc tatctcccta
     7321 agccgtctct aaaacaggct tgtcagtacc ctcaacagca gcctttgtga tacgaacttt
     7381 tgttacatct tcttggcttg gaatttcaaa catgatatca agcatcgttt cttcaataat
     7441 agaacgtaaa ccacgtgcac cagtcttgcg ctcgatagcc ttatcagcga tagccaatag
     7501 agcatcctgg tcaaattcca attctacacc atcataagat aataaggttt ggtattgttt
     7561 aaccaaagca ttgcgtggtt ctgtcagaat acgaaccaga tcttctgcag taagaagttc
     7621 taacgctgca actactggta aacggccaat aaactctgga atcagtccaa acttttgaat
     7681 atcctcagaa attatctctt gcatataaga agcgttgtca tcaattttac ggcttgtctg
     7741 tccaaaacca ataacttttt cgcctaaacg ttgcttcaca aggtcttcaa taccatcaaa
     7801 agcaccaccg acaataaaaa ggatgttctt ggtattaatt tgaatcattt cttggttagg
     7861 atgtttacgc ccaccctgtg ggggaacact tgctaccgta ccctcaataa ttttaagaag
     7921 ggcttgctgt acaccttcac cagacacatc acgtgtgata gaaacatttt cgcctttctt
     7981 agcaatttta tctatttcat caacgtagat aataccacgc tcagcacgtt cgacattata
     8041 atcagcagct tgaatcaatt taagaagaat attctcaaca tcttctccaa cgtatcctgc
     8101 ttcggtcaat gaagtcgcat ctgcaatagc aaacggtaca ttaaggcttt tagccagtgt
     8161 ttgtgctaag aaggtttttc ctgagccagt tggaccaatc atcaaaatgt tggatttttg
     8221 caaatctaca tcatcgtcac tactctcggt ataactaaca cgcttgtaat gattgtagac
     8281 agcaactgct aaagcacgtt tagcacgatc ttgccctaca acatattgat ttaatatttc
     8341 taatagttcc ttaggttttg gtacttctgc taaatcagcc agtacttcct cagctaattc
     8401 ttccttaata atttcttgtg ataaggccac acattcatta caaatgaaaa caccattacc
     8461 tgcaataatt ttttttactt catcttggct tttgccacaa aatgaacaat agacattcat
     8521 atcgttatta cgattcccag ccattttttc ctcaactttt ctattcattt aatttaaaat
     8581 aatgttccat aaaaggcatg gattgtgttg acaaggttag tgaaagcatt catcaaaaaa
     8641 actgttccta atccatatct ttttttcttt atagcaggaa ttgcacataa aacacaaatc
     8701 acagctaaaa atgcggtaaa gaccccaaaa aggctacgtt gcattgaata gtattccttc
     8761 cttatttttc aaaatatgtc acagtaaaag catgagcatt ctgctcatcc ttttcaaaat
     8821 aatctctaga gataacttta aactcagata gattaacatc aggaaaatag gtatcacctt
     8881 tgaattttcc atgaacttta gtttttatga ttgcttcaca ataaggcaga aatgctttat
     8941 aaatacttgc accacctaca ataaataagg tcttattatg ttcctgaaac cattttataa
     9001 cttgttcaac actatttagg actgtcactc catctgcttg gaattgttca tcttttgtta
     9061 agatgattgt ctctctacca ggtaaaacac gacggttcat tccatcaaag gtctttcgtc
     9121 ccataagcaa agcctgcccc atggtcgttt ctttgaagtg atgtaactct ttaggaagcc
     9181 tccatggtaa accgccatta actccaatca aatggtcttc atcttcagcc caaatggcaa
     9241 ttatttgctt tgtcatgact tttttcctca ctaacttatc tctagatatt ctaacaaatt
     9301 ttacctcaaa ttgcttgtta aaaaacatga gatagttaga ttgctaaatc aaaacgtaat
     9361 tgaggtttaa ttgggtcata atcaactaac gcaaaatcat ctggtttaat atcaaaaaag
     9421 tctgttccat ctggaacatt caatactagt ttggggttac actcagacgg ttggcgtttt
     9481 aagagttcct gagcttgttc aaactggtta tcataaatat gaagattatt gataaaatag
     9541 aaaaacttcc caactctcca accaaaatgc tttgcaatca tcatctgaag tgctacatac
     9601 tgcatagcat taatatgatg tgcaactaac atgtcattag aacgttgtgt taatgtggca
     9661 tccaaataaa gctcaccatt aacgcgtctc acatcaaaca tcgtttgaaa agcacaaggt
     9721 agtaggcctt ctgtttcttc aaatgcttca tagtcccata atgaaataac attacgacga
     9781 ttccaagggt tatcttcaag ctgttttaag agttttgata taatattatg ttttttaaca
     9841 actgcaccgt agcgctgacc tatcgtgcca gtatggccaa cttcccaatc attccagtat
     9901 gtcacaccat atttatcatt caaaactgct aaatcatttg tctgatcttg ataaatccag
     9961 aaaatttctt taatagcaga ttttattgga attggacgta gagttgtaat aggaaattca
    10021 ccctttgata aatcatactc tgcaaaggca cctgtaatat acttagaatt agccatttcc
    10081 ccatttttat atctaggtct ggcttgctcg ctaaatacac cttcagacat aattttcgtg
    10141 atattgtctt taaataataa atcggctttt gtcatgagat acctccaaag tcttatttta
    10201 tcagatttta aaaagatatt ctagtgagaa acaagacttt gaaggttaaa gctttttttc
    10261 atatttttag cttctttatg gtaaaatgga atttagcaaa aatgactttt attcagaaag
    10321 gtttaccaat gaaaatagga attgataaga ttggttttgc aaccagtcaa tatgtccttg
    10381 aaatgactga tttggctatt gctcgccaag ttgaccctga aaaattcagc aagggattat
    10441 tgttagattc acttagtatt acgccagtta cagaagatat cgttacactt gcagcttctg
    10501 cagctaatga tattttatct gatgaggaca aagaaactat tgatatggtg attgtcgcta
    10561 cagaatcaag tatcgatcag agtaaggcgg cttctgtcta tgtacatcaa cttttagaga
    10621 ttcaaccttt cgcaaggtca ttcgagatga aagaagcatg ctatagcgca actgctgctc
    10681 ttgattacgc taaattacat gtagaaaaac atccagattc taaagtatta gtgatagctt
    10741 cagatattgc taaatatggc attaaatcta ctggagaatc cactcaaggt gcaggcagta
    10801 tcgctatgct tattagtcaa aatccatcca ttctagagct aaaagaggac catctagcgc
    10861 aaacccgtga tattatggac ttttggcgac caaattattc tgatgttcct tatgttaatg
    10921 gcatgttttc aacaaaacaa tatctagata tgctaaaaac tacttggaaa gtatatcaaa
    10981 aacgttttaa tactagtcta agtgactatg ctgctttttg tttccatatt ccttttccaa
    11041 aattagcttt aaagggcttt aataaaattc tagataataa cttagatgaa caaaagaaag
    11101 ctgaactaca agaaaatttt gagcactcta ttacctatag taagaaaatt ggaaattgct
    11161 atactggctc attgtatcta ggacttctat cgttactaga aaatagtcaa aatttaaaag
    11221 caggtgatca aattgccttc ttttcatacg gtagcggagc tgttgctgaa atttttaccg
    11281 gtcagttagt agacggctac caaaataaac ttcagagcga tcgtatggat caactcaata
    11341 aacgtcaaaa aattacagta actgagtatg agaaactctt ctttgaaaaa acaatcctag
    11401 acgaaaatgg aaatgctaac ttcaacacct accggactgg cactttttct ctagactcta
    11461 tttgcgaaca tcaacgtatc tacaagaaaa ttaataatta atatgacaaa aatttcctgg
    11521 acaggatttt caaaaaaatc ccctgaagaa cgtattcatt accttgaaga acaagatttt
    11581 ttagctgatt cttccctaga aatagtgacg aatcaggact tactctcttt atcactagcc
    11641 aatcaaatgg ctgaaaatgt catcggtcga atagccttac cattttcact tgtcccagat
    11701 gttttggtta acggtaaagt ctatcaagtc ccatatgtca ctgaagaacc gtcagttgtt
    11761 gctgctgcaa gttttgctgc taaaattata aaacgatctg gtggttttct gacaaccgtc
    11821 cataatcgta aaatgattgg acaagtagcc ctttatgatg tacaagatag tcaacacact
    11881 aaagaatcta tattaaatca aaaacagcaa ttattagaaa tagccaacgc tgctcatcca
    11941 tcaatagtca agcgtggagg cggtgcttgc gacctaacta ttgagattaa agaagacttt
    12001 ttgattgttt accttatggt tgatacaaaa gaagccatgg gtgctaatat ggttaacacc
    12061 atgatggaag cattatcttc gccattagaa gacatttcaa aaggcaaaag cttaatgtca
    12121 attttatcta actatgctac cgaatcttta gtgactgcta catgccgagt tgatttaaga
    12181 tttctaagtc gccaaaaaga ggaggccatc aagttagctc aaaaaatgac gatggcaagt
    12241 caattagctc aagtagaccc ttatcgtgct agcacacata ataaaggtat ttttaatggc
    12301 attgatgcta tcgtcttagc tactggaaat gattggcgag ctattgaagc tggagcgcat
    12361 acctatgctg tcaaagatgg gcaatatcga ggattatcta gatggtctta taaggttgat
    12421 gataattgcc tagaaggtac tctgacgcta ccgatgcctg ttgcaacaaa aggtggatcc
    12481 attggtatta accctagtgt ccacctagct cacgatctac taggtcgtcc taatgctaaa
    12541 gagttggcaa gtattattct ttcgattgga ctagctcaaa attttgcggc tcttaaggca
    12601 ttggttagca caggaattca agctggtcac atgaaacttc aagcaaagtc gttggcacta
    12661 cttgctggag ctaaagaaga acaaatttca gaagtcgtta aacagcttct agactctaaa
    12721 cacatgaatc tagagacagc acaaaaaatt gtcaataaac ttactaaaag taaaaaaaga
    12781 atttgagaaa aatcatctca aattcttttt ttacttgact tgaatttcca taagtgtata
    12841 atctagttat aaagactaga ttatatggag ttttaaaatg aataaaaatc aatacccaaa
    12901 atgggaagaa ttacctgaac tagatttgta tcttgaccaa gtacttctct atgtgaatca
    12961 attaattaat cctaaaacaa taactaacga taaactactc accgcttcga tgatcaacaa
    13021 ttatgtcaaa cataattata tatccaaacc tataaaaaaa aaatacaatc gccgtcaagt
    13081 agcacgattg attgttatta cagcttttaa acaagttttt gctattcaag aaatcagtca
    13141 aacattagaa cttctgacag ctgacaatca ttctgaagaa gcatataacg gatttgctgc
    13201 ttgtatgaac aaagaagagg ttcacgattt accaccagtt gtcattagtg cctgtcagac
    13261 gttgaacctt tatcaagaaa cacaaaaatt agttttagaa ctaggaggaa aaaataaaaa
    13321 tgcttaaaga aatgtctatc aaattaagtc ctcaactttc ctttggcgaa gagcttgcaa
    13381 atagtatcac tcacgctgta ggagctttgc ttatgctaat cctattacca atcaccgctg
    13441 tctatagtca caaccacttc ggattacaag cagcacttgg tacctcaata ttcgtcactt
    13501 ctttattttt gatgttttta tcatcaagta tttaccactc catgacttat aacagtcttc
    13561 aaaaatatgt tctacgcatg attgatcact ctatgattta tattgctatt gcaggtagtt
    13621 atactcctgt cgcattgtca cttattggcg gctggttagg atatctcatc atttttttac
    13681 aatgggggat tacattattt ggtatccttt ataaaatctt cgctcccaaa atcaatgata
    13741 aatttagcct tgtactttac ctcattatgg gatggctggt tatctttatt ttccctgcta
    13801 ttatcacaaa aactggacca gctttttggg gattactctt ggctggtggt atttgctata
    13861 ctatcggtgc actcttttat gctcgcaaac gtccttatga tcacatgatt tggcaccttt
    13921 ttattctatt agcttctatt ttacaataca ttggaattgt ctacttcatg ctataaaatt
    13981 taactacatt ttattgatta tttttttgct aaaccatgat ataataataa tcatctaaat
    14041 cttagagaga aggcgtggac atatgaaaag tgcttatatc ttttttaatc caaaatctgg
    14101 taaagatgaa caagcattgg caaaagaagt aaaatcttat ttaatagaac atgattttca
    14161 agacgattat gttcgtatta ttacaccttc atctgttgag gaagctgtcg ccttagcaaa
    14221 aaaagcatct gaagaccata tcgacttggt tattccttta gggggagatg gcactattaa
    14281 taaaatctgt ggtggtgtct acgctggtgg tgcttatcct actatcggac tagtacctgc
    14341 cgggaccgtc aataactttt caaaagcttt gaacatcccg caagagagga atctcgctct
    14401 tgaaaacctt ctaaacggtc atgtaaagtc cgtagatatc tgtaaagtta atgacgatta
    14461 catgattagc agtttaacac ttggtctcct tgctgatatt gctgctaatg tcacttctga
    14521 aatgaagcgt aaattaggcc cttttgcttt cttaggtgat gcctaccgta tattaaagcg
    14581 taatcgatca tattctatca ctcttgctta cgataataat gtacggtctt tacgcacgcg
    14641 gttactcctt attaccatga ccaattcgat tgccggtatg ccagccttct ctccagaagc
    14701 aacaattgat gatggacttt ttagagtcta cactatggaa catattcact tcttcaaatt
    14761 attgttgcat ctaagacaat ttcgtaaggg agattttagt caagcaaagg agataaaaca
    14821 ttttcatacc aataatctga ctatttcaac ttttaaacgt aaaaaatcag ctatccctaa
    14881 agttcgtatt gatggcgatc caggtgacca attacctgta aaagttgaag tcatccctaa
    14941 agccttaaag tttatcattc ctaactcatt accataatat aaaaggacct agacataagt
    15001 attagagtaa atactatctg tctaggttct tttttgttat acttaaagcg ataaatatca
    15061 ttgaaaggaa gtatacgatg acaacattta ctgctaaatt tattgaccaa gagtgggaag
    15121 tcccagtaga atcaggtcgc taccatatga ttgttgggga attttgtcct tatgcacaac
    15181 gtccgcagat tgcgcgccaa ctgcttggtt tggataaaca tatttctatc agttttgtgg
    15241 atgatgttcc aagtgatata ggacttattt ttagccaacc agaacaggtc actggtgcta
    15301 agtcgcttcg tgatatttat catttgactg acccgaccta ccaaggtcct tatactatcc
    15361 caattttaat tgacaagacc gataatcgta ttgtctgtaa ggaatctgca gatttgcttc
    15421 gactttttac tacagatttc tctgatttgc atcaagagga tgctcctgtc cttttcagcc
    15481 aagaaactgc ttcacttatt gataatgaca ttaaagacat taataagaac ttccaaagct
    15541 taatgtataa gttagctttt cttgataaac aagcagatta tgatacttat agtaaggaat
    15601 tcttcacttt tctagatcaa aaagaacact tactaggaca acgtcctttc ttgctcggag
    15661 ataatctaag tgaggttgat attcatttct ttacaccatt agtccgatgg gatattgctg
    15721 ggcgtgatct tctactactt aatcagaaag cgctagaaga ctacccaaat attttttctt
    15781 gggctaaaac tttatacaat gactttaatt taaaaacatt aaccaatcca caatccataa
    15841 aaaataacta ttacttaggc aaatttggaa gagctgttcg tcatcatacc atcgtcccaa
    15901 caggtccaaa tatggcaaaa tgggaaaaat aaagctaaaa agctaaggca attcacagcc
    15961 ttagcttttt agctatacta gtccaaagtg gaccaatcaa attcaataaa agtcttgaag
    16021 agatctccat aagtaccact gtaagcaata tctttatctt tataagtcat tttcgtaata
    16081 ggataattgt caggaacagt aacacatccc attaaaactc gaataaaatc atttggattt
    16141 tcaaaaactt ggcatcgttc agtgtcgtaa gcatctttat aatacagttt aatcatctta
    16201 ttacctctga cgttttacaa ggaaactttg acaggaagct atatctactt cacaaaaata
    16261 atatcattta gcattggctt ccttcaattg accatacaat atataattga cttgtcgtaa
    16321 atcagttatt tttttacaat tcagtgcaca cattatcatt cttaaatctt ctttccaaga
    16381 attgagaatt gcaataacat catctacagg ataacgctca actaattcta aaactgttct
    16441 agaaagtcca acagctttag cacccaacac taaacatttt accatatcta aaggatgacg
    16501 aatgccacca gatgctagga tatccatttt gtccatcata gattgagcgt ttattaagga
    16561 ttgagctgtt gtttgccccc aggtattgag atacgaacgg tcgcgtccac gttgattttc
    16621 gatgtaagca aaactagtcc ccccacgccc cgaaatatct acagttgtga tacctatatc
    16681 gtaagcatcc tttattgatt gtaaatccat cccaaaacca acctctttta aaataagtgg
    16741 gcattggata ttgtcgacgt attcttttaa gtgagaacgc cacatgtgaa attcacgctc
    16801 tccttcaggc atcaaaagtt cttgcatcac attaacatga acttgtaaaa aaatggggtt
    16861 catagcctta acggttgact ctgctgctgg tacaggcttg tctaacccta tatttgtcgc
    16921 cagttttaaa tcaggataca agtctgttgt tgggtaggaa tcatcttcat cgtttttgag
    16981 agctgccgaa tatgaacctg tcaccataac aatccctgta gcttgagcaa cctgtgccaa
    17041 tttatgattg actgctttcc ctttttcact accgcctgtc atagcattga tataaaaagg
    17101 aaattcaaag ctttgaccag caaaatgtgt tgataaatcg atatcgttga cattatattt
    17161 tggtaaggat gaatgtatta actcaatatc gtcaaaacta ttataatgcg actgatattt
    17221 aagagcatat tttatgtgat catcttttcg atttgtcatg acagtgtctc cttactaagt
    17281 agttcaatac cagcactctc ccaacgcttg attagagttt gattatcatt tttattaaag
    17341 gatatggcta taccacaatc gcctcctcca gatcctgaag attttgtgac tatatctagg
    17401 cctttgcttg cctctttaag cttttgaagt ttatcggtgt aaatagatgg atgaagatta
    17461 aataatagca agctaatttc ttctaaacat ctcttaatcg cttctttatc tccttcttta
    17521 aaggctgaaa gtaactggac aacttgatac tttgtatcat ctaaaaaacg ctgcgtaata
    17581 gttgatttaa ctatatttat catttctttt gaaatggacg gttgcattgt ccatcccact
    17641 aaaaaatcca tctccaataa agcttgtatt ttggtaatac gataacccca ttcagcttcc
    17701 aatacttgtt ccaaaggttt tgtttcaatc atttttgaaa cagccctacg atcaaaagct
    17761 gaataagaaa ttaaatgctc ataagcaata caagcaatat ctcccattga gccattatca
    17821 cctcttttta agagaacata ggcagataat ttaaaaagca agtcgcttgg catctctatc
    17881 tcataaagcg cagccatagc tttaatagtc aaaacgacaa cacttccgct agagcctata
    17941 ccaaatttaa gtccatagcg ctctaatttt cccgtaattt cgaggtgaat cggttttata
    18001 tttttgccaa aattaatcaa ccattcttcc ataagcaata tcgtttcctg aatgagacta
    18061 tattgcttat ctggctgtaa gcttgccgta tagttaaaca tatcagaata aaggctgtag
    18121 ttatctgcaa aagtagctaa tgcagtcata taaataggaa cgttctttaa aatagctact
    18181 tgcccaggat acagtatcgc gtattcacct gcaatatata atttgccacc tgtttgcacc
    18241 ttaaccatct gctaattcct ttgtcgttga aacaataata cgataatctt tctccaagat
    18301 agcagctaat gcctcaaggt cttgacgtaa gcaaagtact ttaacatttg gacccgcatc
    18361 catcgtataa taacattcat gacctttttc gcgaagtttc ttcacaacat ccatagcttt
    18421 atatgtctct tctgtcaaat atgaaaaggc tggagttgct gttttagttg ttgagtgcat
    18481 tgcaagtgca ttacgctctg tcagttgacc taccttttga aaatcattat tttttaggta
    18541 tactaacata tcttgataat cttgttcaga ttgccttacc cattcattaa aagtagttga
    18601 agtttcggtg cagattttca taccttcacg gcttgaaatc ggctttcttt tgtcatttaa
    18661 gaccagcata atcatagcta aatctaaatt agtgtgaact ttatagatat caccactatc
    18721 tttatcccaa gcagcaacag gtccaaaaaa agagcgtgaa gaggagcctg aagcaaactt
    18781 ggcttcttga gctagctgtg attggctaag gttagtacca aaaaaatcat tgcaggcctt
    18841 tactaaggct gataaaccac ttgatgacga tgataagcct gcagctgttg gcatattatt
    18901 agttgtttca atctttacaa agccggacct attttgtcga aagcgatcca agatagctga
    18961 cattttgtca tgttcatgat cattttgaag aacaccagag atgtaaaatt catctttctt
    19021 agcatccttc ccaagggcag tcaaacgtgt ttcagtgtac atgttttcaa gtgttagaga
    19081 aatactactg gtagctggaa tcattttttc agcatctgct tttccccaat atttgataat
    19141 agcaatgtta gcataagact taacacttat actttttccg tccatgtgtt gacagctcct
    19201 tctctttcta attgctgact taagcgctca gcttctcttt tttcttttac aagtgcgata
    19261 atacaaccac ctaatccgcc gccactcatt ttagcgccta gagcaccaca actaatagct
    19321 acctctacca gatgatcagc ttctaaactt gagacaccca attgttttaa actttgatgt
    19381 gctttagtca tcaagctacc aagttgtttg gaatcacttt tatgaatagc atcctctgat
    19441 gcctctgcta aataacctaa ttcttttaaa aagggtagta ctgcctcgcc agatgatttc
    19501 accttatcca ctgcttcttt agtatgccca tgaattcctg tatcagcaat aactaaataa
    19561 gcattcaaat gcatgggtac tgttgaaaaa ccaatattgc ggataaattt aatagtattt
    19621 tcactaagac aagtttttgc atctaatcca ctcggatttg aatgtgctat catttctgct
    19681 cgattaacaa ggcattctaa caaatctgcc tccaaatctt catcaaaata atcaaaaact
    19741 gcacgaatag ctgcaatagc aactgcagct gatgatccca taccacgtct ttctgggact
    19801 tgtgactcaa tatgatatgc aattttagaa gaagttttac ctaaatagtc caaggcagca
    19861 aaaatggctg tcgacaaagg atcagtcata tctaatgcta ttaattgagg agcctcttca
    19921 ataaggcaag ttacttcaat attttttaaa ggaatagcaa tggctggata accatatact
    19981 actgagtgct cacccatcaa gataatcttg ctatgagcct ttcctatacc aaacttttct
    20041 ttcatctttt tctctttaca attctattac cttactattt taatataaat ccttaaaaaa
    20101 agcgacttta acgtgataat aacataaatt aaaggaacta caattgcagt ccctttaatc
    20161 ttaaatgtaa atgacaaagg taatggtatc atgtttataa ttcacatcga tacttccttt
    20221 aaatagatgc accaaactag ttacgataga aagaccaata ccatatcccg attttttgtt
    20281 attatgtgac tcatcctctc gataaaatcg ttcaaaaaac ttgctatagt ctatgtcttt
    20341 accattctta taagtatttg aaacttcaag cttagctcgt ctcaaacgac ttgaacgtga
    20401 taacttaact gtcacagtcc ccataggatc acaatactta ttagcattat caaccaataa
    20461 tgtaaccaat tcaaataatg atttttcttc agctttgaca tgaatacctg gttggatact
    20521 cattataaag tctttaccat ctttaatgat tggaccttta aaatcttctg cagcatcttt
    20581 tgtaatatgt gaaaagtcta agtcaaccat acttatatca ggctgttctt caaagcgggc
    20641 aagactgacc atgccattaa ttagacccgt taaacgttgg atttgatcat ttgtagattt
    20701 agtccactct gattcaccac tcatcatttc caccaattca ttattagcgg agatgatagc
    20761 aagtggagtt tttagttcat gtcctgcatt ggtaataaaa cgcctttgct tttcatagtt
    20821 agcaacaaat ggtaagataa ctctccctga taaaactgaa acaattacca tgaagacgat
    20881 aaaacttacc aaggacatcc atattgaaag ttgcaataat ctttgattat caagatattg
    20941 gttggttgta tctaaaaata ccactaaata atcattttta ccaacctttt ttagtagata
    21001 agaataagta ctaaaattat aggtgatatg acctgaatga ctattttgtg atacgatacg
    21061 cttggcataa ttttttattt gcctatcgct tacttcaaag atatttctag aatcaaaact
    21121 agtcacgtta ccttttgcat ctgttcgcgc actgaaataa cggaattgga aaatagcgtc
    21181 cgttgatagg tcatcaccta attctttact aaattcaagt ttattcggta tacgtccatt
    21241 gttgtctgca aggatagtta acacacgata aatttcattc ttcgtttgat agtgattagc
    21301 agagttgagg acaccgacta ctgaaaataa gacaacaaga atagctagcg cagctattcc
    21361 gataaaacga agtcgtaaat ttctaaacat tagccaccat ttatttcact taatttatag
    21421 ctactacctt cttgaccaat aatctccaag ttagcaccaa ttgcctgcaa tttttgtctc
    21481 aaataagaaa catagataaa gacgtatccc tcatctacat cttcttggtc cttatctgct
    21541 ccccagacat gttcgaaaag ttgttgtgta gataattctt tatcatggtt caacatgaaa
    21601 aatgctaaca ttttagcttc tttacccgct agacgaatag tgttttcaca ttgcaactct
    21661 tgttcaccga ctgataaggt tactcttccc aatgacaata cattaggtgt gaaatcttcc
    21721 aatcttcggg acatagagcg aagccttgct aataattctt ttagagagaa gggttttgtt
    21781 aaataatcat ctgcaccagc atctagacct gttactctat catcaatttc tgccatggct
    21841 gttagcataa taatgtgaga tttatttcct gattgtctaa tttctttgac agcctcaaca
    21901 cctgttttaa caggcatcat gacatccata accatgacat catatgcatt ttgattagct
    21961 aaatcaatag cagtctgacc atcgtaggcg acatcaacaa cataaccttg atgactaatt
    22021 gccgtactaa ggacacgtga catctgttct tcatcttctg caattaatac tctcatttta
    22081 ttccttcttt gttccactct ctatcaattg acgaatcgtt tgcatcgtta aatcaacaga
    22141 tgctatttca tcagcacaac gttttaattc tcttacaatt cgttcgggat tttcaatatc
    22201 atgcttgtat ttcatctgat gttctaaact agcccaagaa tcttgcgcaa ttgtacgcaa
    22261 ctgaatctcc atgtaatatt taccttctga attacccaaa cagtccggat agggagtatc
    22321 aatttctaaa atcacatggt aagagcgata cccatttggc ttaacatgct ggatatagtc
    22381 tttttcaact acaatacgac aatcatcaaa tgtcttaata cgttctacaa tttgataaat
    22441 gtcgttaaca aaaccacaaa taatacggac tccaatacta tctctaataa cttttagtgc
    22501 agaatctact gttacaggta actgtttacg acgacatttc tctatcatac tatctgatgt
    22561 cttaatacga ccaatgatat gttcgtacaa tttatagcct gtttcaacct tagctttatc
    22621 attttctaat tggatacgct gactgaaatc ttctaaaatt aatggtagat atcgaccata
    22681 atctccataa atatttgttt ccataagaaa ccttcctatt ctctcttaag ctccattata
    22741 tcatagaaac ttaaggcaaa ctaaaaagga atgagaattt tttcttccca tttccttcta
    22801 aaatgtttat gttaagctga ttcgaattga gaattataca gatcagcata aaaaccactt
    22861 tgagacatca aaacatcatg attgccttgc tcaatgatat tcccatcttt catgaccaaa
    22921 atcaagtctg cgtttttaat cgttgataaa cgatgtgcga taacaaatga tgttcttcct
    22981 tccataagtt tgtccatagc tttttgaatt aactcttctg tacgtgtatc aacagatgaa
    23041 gttgcttcat ctaagataag taagggagaa tttttcaaaa gagctcgagc aatggttaat
    23101 aattgctttt gtccaactga caatgtcaca gcgtcatcaa ggacagtatc atacccttta
    23161 ggtagtgtca ttataaaatg atgtataccg actgccttag cagcttcttg aacctcatca
    23221 tcactgattg tcgtctggtt aaaaatcaaa ttttctttaa tagttccctc aaacaaccat
    23281 gtatcttgaa gaaccatcga gaaagcatca tgaacttgac tacgtttcat gtcttggata
    23341 ttgatgtcat ctatggaaat actacctgaa ttaacatcat aaaaacgcat caataaatta
    23401 acaattgtcg ttttacctgc gccagttggt cctacaatag caactttctg accaggctgt
    23461 gcctccgaag taaagtcatg aataatagtc ttgttatcac tataaccaaa cttgacatgt
    23521 tcaaaggaca catgcccctt aaacttagga agatttaaca ctttgtgttt atcctcttgc
    23581 atatttgttt catttaaaaa ttcaaaaaca cgtcccatgg ctgcactagc agactgtaat
    23641 tgtgtaattc cttgagcaat ctgaccaata ggctgtgtaa aaatacgaac ataggtcata
    23701 aaagcaacaa ttgtacccat agtaattgtt cctttgatag ctaacatggc accaaccaca
    23761 cacaccatca cataaccaaa attaccaata aactgcatca gaggcatcat aattccagag
    23821 aaaaattgtg atttccacat ggaactgtga agtttatcat ttaatagttg aaaatctgac
    23881 ttagcctgct gtcttgcatt atgactagta acaatgttgt gtcctgagta aatttcttct
    23941 acatatccag atatattagc caaattcacc tgttgctttt taaataaagg ctgactcttc
    24001 accataatga ttgaagaaag agcgaatcct gaaaataccg atataatcgc cgttgttgct
    24061 aaatgccaat ctgttttgaa catcataatt aatgaaccaa ccaataacac tgtagatgca
    24121 accatagtaa ctaaactttg gttaaaagac tgtgtcatca aatccacatc atttgtaaca
    24181 cgagataaag tatcaccttg agtatggcta tcaaaataac tcaaaggtat tttattgatt
    24241 ttatcagcaa tagcattgcg cataccttga gcaaattttt gaataatagt cgtcacaata
    24301 aaacttgcta gataggatac taatgcacct aaaacatata atactgccaa ttgccctgca
    24361 atatgaccga ttttggctaa gtcaatatta ccagccaagc cttttaccat aagatccgtc
    24421 atctctttaa gacggtctgg tcctataact gttgtcaagc ttgataaagt tgctaacaca
    24481 attgctaacg caaaaagaag tttgtaacct ttgaggtacg gagataaatt agagaaaaaa
    24541 gacgtttttt gtgatttagt tttcattttc caattcctcc ttagacaatt gagaataggc
    24601 tatctcttga tatacttcat tatttgataa caattcctta tgtgttcctt gcccaacaac
    24661 tttaccttga tctagcacta aaatgagatc agcatccatg atagttgaaa tgcgttgagc
    24721 gacaattaat ttcgtcatag cagctgtttt ttctttcagc acatggcgta aaatatgatc
    24781 tgttttataa tctaacgctg aaaatgagtc atcaaaaatg agaatttcag ggtgacgtgc
    24841 taaagcacgt gcgattgcca agcgttgttt ttgtcctcct gaaaagttac taccaccttg
    24901 agcgacttct gtatctaatc ctttttcttt agcttctata aattctttag cttgcgctaa
    24961 ttcgagtgct tgccaaattt gttcatcact aagttcctcc ctgccactct ctccaaaaat
    25021 catattagaa cgtactgtcc cactaaagag aacagctttc tgtggaatat agccaatttt
    25081 attattcaaa tcattgtgag agtattcctt tatatcaatg ccatcaagtt taattgaccc
    25141 ctctgtggcg tcataaaaac gtggaactaa attgactaaa gtggattttc cagaacctgt
    25201 agacccaata aatgccactg tctgaccagt atctgctttg aaagatacat gttcaataac
    25261 cgcttcagaa tctttactat aatgaaatga cacatcttga aactcaattt gaccttttgg
    25321 tgcactttgg atatgactat tttctttaaa agttactgtt ggggctaaag ttagtacctc
    25381 attaatacgt ctagccgata ctaaagcacg aggcatgatg ataaaaatag ctaccatcat
    25441 cataaatccc ataacaactt gcatagcata agatgaaaaa acaaccatgt ttgaaaaaag
    25501 atccactcga tcagtgatag ctgatttagc agccaataca gatacacttg ttcctgtaac
    25561 tttaggcaca gaaatatcat taatcaaata agagccaatc caataaatag ctaaagtcaa
    25621 tccagatgaa accactgtca tcacaggatt cataagcgac atcaatctat aaacaaaaag
    25681 gttcaatttt gttaaatcgt cattttcacg tttgaactta tctgtttgat acttttcagc
    25741 attgtaggca cgaataactc taacaccaga gagtgcctct ctagtagttg cattaagcgc
    25801 atctgtaaga ccttggattt gacgttgtct cggaaaagca agtaagagta gaacgctaag
    25861 taatatcata acgataataa ccgctactcc aactgcccca gtccattgat cgcttttacc
    25921 cccaatttta gtcaaggccc atattgccat aattggtcca cgggtaacaa cctgcattcc
    25981 cattacaata aggatctgta gctgagtcaa gtcgttagtt gtgcgcgtta atagagaggg
    26041 gatagaaaat cgcttaattt ctgcatcaga ataatccatc acatggttaa aaatatctga
    26101 acgtaatcga ctagtaaaac tagctgctgt cctagcacct aaaaaaccta ccattactgc
    26161 tactaaaaaa ctaccaaatg aaaaagcaag catttttagt ccaggatccc aaagagcctc
    26221 taactttgta cctttagttt gcaacatacg tgtgatatca gacatatatt ctggagtctt
    26281 taggtccatc caaaccgcta gacacactaa tatagtactc gccacaatca tcaaccattc
    26341 tttaccattg agtcgcttga aaatattaaa catcgcaaac tccttttact ttcatattta
    26401 tcctattatc ctaaaatagt taatcaaaat cttccatcgc catattttta gctaatgttc
    26461 taataacctt tttggtagta cgtatctctt ctttagtaat acctgctaac atggtactgt
    26521 gcaatttttc taagaaaatt tcaaattgtg tagccttttg tttacctaga tgagtcaaat
    26581 aaaggtattt cacacgctta tctgtcttag atggaacaat cgcaataaat ccattttttt
    26641 ccatccgttt taccaaatta gacgctacag acttagaaat gtgtaaaatt tcttcagcat
    26701 cttttataga catatccttg tcaggatgtt tatagagatg catcactaag tgtccttgtg
    26761 gacccgctaa atgttcaaca tcatactctt ttgcataacg atctaaatac ttttcaagtt
    26821 gattaacaag tattcgtgct ttttgaagag gattctccat aataatactc ctttaatagt
    26881 tatcgtgaga actattttat aatagctaaa ttttattgtc aagaaaaaga ttctcataga
    26941 aactattttt ttcaaaacat cattatttcc tttttttaaa atatctcccc actatttttc
    27001 ctaataaagc gttctccatt taatgacttc tacgcaatgt tagcttacaa aaaggaactg
    27061 agtaatatta ctcagctccc aataaactat cttgctaact tctcttttaa ataatatcct
    27121 gtataggatt taggattttc agcaacttct tctggtgttc cgatagctac aatttgtccg
    27181 ccaccaatac ctccttcagg gccaagatca ataatgtggt cagcagtttt aataacatca
    27241 aggttatgct caataacaag cactgtatta ccatcatcta caaaacgatc caaaacctta
    27301 agtaaacgag caatatcatc tgcatgaagc cccgttgttg gttcgtctaa tatgtaaaga
    27361 ctcttacctg tcgaacgctt atgtaattca cttgccagct tcattcgctg agcttctcct
    27421 cctgataagg ttgtagctgg ttgccccaaa gtgacatatc ccaaaccaac atccttaata
    27481 gtctgtaact tacgagcaat tttaggaata gctgcaaaaa atgtaactgc atcattcact
    27541 gtcatatcaa gaatttgtgc aatatttttt tctttgtaat gtacttccag agtctcacta
    27601 ttatagcgcg tgccgtggca tacttcacaa ggaacgtaga catctggtaa aaaatgcatc
    27661 tcaattttga tgataccatc gccagaacaa gactcacaac gtccaccttt gacgttaaat
    27721 gagaagcgtc cttttttata acctcgaatc tttgcctcat tagtttgagc aaaaagatca
    27781 cgaatatcat caaaaacacc tgtataggtt gcgggattag aacgtggagt ccgaccaatt
    27841 ggactttgat caatatcaat caatcgatca acatattcaa tgccttctaa agaaacatat
    27901 ttacctggtt tatctgagtt acggttaagc ttctgcgcaa cagctttttt caaaatacta
    27961 ttaattaagg ttgattttcc agaacctgag acaccagtta cggcaataaa tttaccgaga
    28021 gggaacttga catctaaatt ttgtaggttg ttttctgccg cacctttgat ttctaaaaag
    28081 cgaccattac ctactcttcg ttcagatggc acaggaatca ctttcttacc tgacaaatat
    28141 tgtcctgtaa tagatttagt atttttagct acttgcttcg gtgtaccaga ggccacaatc
    28201 tcaccaccaa acgcaccagc gccaggtccc acatcgatta accaatcagc tgccatcatt
    28261 gtatcttcat catgttcaac aacaataagc gtattaccca aatcacgcat tttcttaaga
    28321 ctatcaatca aacggtcatt gtctcgctga tgaagtccaa tggacggctc atcaagtaca
    28381 taaaggacac ctgataaatt agacccaatt tgagtagcca aacgaatacg ttgactctcg
    28441 cccccggaaa gtgttcctgc agaacgtgac agatttaaat aattaagtcc aacattattt
    28501 aagaaactta gacggtcatg gatttctttg ataattggtc gagcaattaa ttgttcattt
    28561 gaagataaac gcaatgtttc taaaagttct aaatggtctg ctatcgataa atctgagact
    28621 tgcccaatat taagaccttc ttctccccca actcttacgc aaagtgcttg atcattgaga
    28681 cgatagccat gacaagtgtt acattttagc tcgttcatat attctcgcat aacattgcgt
    28741 gtgtaatcgc tattagtttc atggtaacga cgattaatat tattgacaac cccttcaaaa
    28801 ggcaaatcaa tatttctctc gcctccaaaa tcgttaatat aatggaaatg gaactctcgt
    28861 tcgcctgaac catatagggc taggtcctgc tccgccttgc ttaatttttc aaatggcgta
    28921 tccacatcaa caccaaattg agtcatagct tgctctagca tcgttggata ataatttgat
    28981 gaaattggat tccaaggaac taaggcccct tctctaaggg tcttacttct atctggaata
    29041 accaaatcta tatctacttc taacttaatt cctaagccat cacaggtcgg acaagaacca
    29101 aagggagcat taaaagaaaa aaggcgtggc tctaattcag gaacagtaaa accacattca
    29161 ggacaagaat aatgctctga aaacaataac tcattgccat ccattgtatc aataacgaca
    29221 taaccatccg acaaacgtag agccgcttca acggaatcaa ataagcggga acgaatgccc
    29281 tctttattaa ttaatcgatc tactacaata tcaatattgt gcatcttact ttttgataat
    29341 tcaggcacct cagtaacatc gtgaatatca ccatcaatac gaacacgaac gtaaccatct
    29401 ttttgtattt tctcaaatgt tgatttgtgt tgtccttttt ttcgacgtat aattggggct
    29461 aaaatttgca tctttgttcg ctctggaagc gctagtacct tatccacaat ttgctctaca
    29521 gaagatgctg tgatggctcc gtgaccattg atacaatatg gggttccaac acgagcataa
    29581 agcaaacgta aataatcgtt aatttctgtt acagtcccaa cagtagagcg aggattctta
    29641 cttgttgttt tctgatcaat agaaatagct ggactaaggc catcaataga atctacatca
    29701 ggcttttcca tatttcccaa aaattggcgc gcgtaagcag aaagactttc aacgtaacga
    29761 cgttgtcctt ctgcatagat agtgtcaaag gccaaactag acttacctga tcctgatagt
    29821 cccgtaacga caactaactt gtctcgtgga atgtcgacac ttatattttt taaattgtga
    29881 gctcttgctc ctcttatcat taatttatct tgcataatgt tgtgcctcag cactcctttt
    29941 tgaatagtgt tgacttatta tatcaaattt taataaagtc agtatttttt tagttttaaa
    30001 tttcatagaa aaaagagatg aactaatacg ttcatactct ttgttattat cttctttgtt
    30061 gtggtgccac tggtgccatt gagctttgaa tattatattt acgttgtaaa taaaagcgca
    30121 aaccaaacga tgctaaagca ataataaaca aaacaacctc aggaagttta ggattaatag
    30181 ttgcaggtag aagaggtact actgcaaata aggcaaacca aactaacata actagtgcca
    30241 gtactgcgaa agatttaagc cagcctggtc tttcagattt tggtttaccc atatggcgat
    30301 aaatatagta ataagtaacg tacatggccg caccaccacc aaaaccaaga gttaaaattg
    30361 aaataagccc tgtattgaca ttagattgac caaatagcaa cattaaacca ttcaaagcag
    30421 ttactaagcc aagaaatagt aaggaagtat ctaaccacat caaccaagga ttggtatttt
    30481 tttgtacaga tactctagct ttatcctcag gtcgctcagt gaatgaggct gcccaagtag
    30541 ttggtgctcc taagagtgag cgtgcggtaa ttcctttttt ctgattatct ataatctctg
    30601 ggagatgttc ttctaaaata gctttgattt ctgcatctga ttttccatca gctaaaagtt
    30661 gattagttgc aatatgaaca aactcttgat tctttttagt taagtcttgt aaagtcatgt
    30721 gaactccttg attaatatac tattaaaacc attttttacg aataaaataa aatacaacaa
    30781 atgaactcat caaaaaggcc aaaagaacaa catagataaa gccatgtgcg agaccattca
    30841 aaggcatcca attattttta aagttcatcc catacgccga aaaaataacc gttggaatat
    30901 ccaatgtcat agttactagg gctaaggttt tcataatggt attctgatta ttaccgatga
    30961 ttgaagcagt cgtttctgtc atggcattta agacattttc atagatattt gccatttcaa
    31021 tagcctgctg agtttcgatc aatgtatctt caagcagatc ttcatcttca atgtattttt
    31081 ttaaactact ggtactactg gtcaattttt tgacgattcg ctcattaaat tttaaagagg
    31141 cctttagata gacaatagat ttttccaatt ccatcatatc gatcaattgc tcatttcgag
    31201 ttgcactctc tagttgtgct tcaattttat cgctctgcct atcgatagta cgcaaagcct
    31261 gtaagtataa ttcggcattg cgataaagca attggaaaac aaaacgtgtc ttcataaagg
    31321 tataaaaatt tttgacacgt cgtctataaa agtgatcaaa cagcgtcaaa tgttctaggc
    31381 aagtagtaat aacagcgttg tcagtaacga taatgcccat aggaatagtc atataataac
    31441 ttttgttatt gcgttcttca taggtaggaa cgtcaacaat aactaaagta taatcatctt
    31501 caaccgagat acgggaagtt tcttccacat cgaggggagc acgtaaatca tcaatatcaa
    31561 taccaaattg atcagctact tgttcggatt cttcctggga gggattaacc agatttatcc
    31621 aagcacctgg ctcgaatgtt tcaatttctt taaattcaat tgccgttgaa agaaacattt
    31681 gcttcatggc gattcctcct ttgttataaa aaatccatac cttagtatta tacaggaaaa
    31741 ccatttgttt tgcacggtta atacggtggt atcctaaatt tttctattat ttgactcgta
    31801 aataccaata ataatggtaa aaagaataaa aaaatagccg ctagggctat tttatattaa
    31861 tcttcattta cgtaaggcat aagagccata acacgagcac gtttgattgc tgttgttact
    31921 ttacgttggt ttttagctga agttcctgtt acacgacgtg gtaaaatttt tccacgttct
    31981 gaaacgaaac ggctaagaag ctctgtatct ttgtaatcaa catattcaat cttgttagct
    32041 gcgatataat caactttttt acggcgtttg aatccgccac gacgttgttg agccatgttt
    32101 atttctcctt tatagtttta aattctgtcc atctattata ttagaatggt agatcatcat
    32161 ctgaaatatc cattggattt gagttaccaa acggactctc atcacgacta aaattaggtg
    32221 tttgttgaga agaagctgaa taactattat ttgacggagc attgttatag ccaccgttat
    32281 tataagagtt aggtgaacca ccttctcgtg tagcacgact ttccaataat tggaaacttt
    32341 ccgcaacaac ttctgttaca tagacacgtt gaccttgttg gttttcataa ttacgcgttt
    32401 ggatacgacc tgtaattcca accaaagcac ctttttttgc ccagttagcc aagttttcag
    32461 cttgttggcg ccaaataaca cagttaataa aatcagcctc acgttcgcca gattgatttt
    32521 taaaattacg attaactgca agtgaaaaag tggctaccgc ttgattactt ggtgtataac
    32581 gaagttctgc atcacgggtc atgcgaccta caagtactac attattaatc ataaactacc
    32641 ttcttagtct actttaacga tcatgtgacg aagaatgtca ccgttgattt ttgaaagacg
    32701 atcaaactca ttaagagcta ctgcatcttc agcttcaacg ttaacgatat ggtaaagtcc
    32761 ttcagtgaaa tcttggattt cgtatgcaag acgacgtttt tcccaatctt ttgattcaac
    32821 aacagttgca ccgttgtcag ataagataga atcaaaacgt gctaccaaag cgtttttagc
    32881 ttcttcttca atgtttggac gaataatata aagaatttcg tatttagcca ttgatatgtt
    32941 cctccttttg gtctaatgac tcatgaattt ttcatgagta agtgaggtta tgctcacaag
    33001 atattattat agtagaaata agttaataat gcaaggtttt tatgttttta tttccctgat
    33061 atcatttaaa gacatttttt atttttcttt attgaaatat gccttatatc aaacctatgt
    33121 agccattttc ttttactgac ttatgttcaa tccccattct ataattcaat taaaaaataa
    33181 aaaggcgagg tttacacctc accttcaaca tattatttaa agaattcttt attggtttaa
    33241 gttttcgttg gagaaaaatt cagtgtctcc gatttt
//
LOCUS       DATRME010000013        32423 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRME010000013 DATRME010000000
VERSION     DATRME010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32423
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..451
                     /gene="atpB"
                     /locus_tag="VBC23_000648"
     CDS             <1..451
                     /gene="atpB"
                     /locus_tag="VBC23_000648"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6842617.1"
                     /translation="NNLGLMTSLKTHEHNFWTSPTANFGVDITLSLLVAFICHIEGIR
                     KKGIGGYLKGFLSPTPAMLPMNLLEEVTNVASLALRLFGNIFSGEVVTGLLLQLAVLS
                     PFTGPLAFALNIVWTAFSMFIGFIQAYVFIILSSSYIGHKVHGDEEE"
     gene            469..966
                     /gene="atpF"
                     /locus_tag="VBC23_000649"
     CDS             469..966
                     /gene="atpF"
                     /locus_tag="VBC23_000649"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6842618.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            966..1502
                     /locus_tag="VBC23_000650"
     CDS             966..1502
                     /locus_tag="VBC23_000650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6842619.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            1518..3023
                     /locus_tag="VBC23_000651"
     CDS             1518..3023
                     /locus_tag="VBC23_000651"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6842620.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            3039..3920
                     /locus_tag="VBC23_000652"
     CDS             3039..3920
                     /locus_tag="VBC23_000652"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6842621.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            3994..5400
                     /gene="atpD"
                     /locus_tag="VBC23_000653"
     CDS             3994..5400
                     /gene="atpD"
                     /locus_tag="VBC23_000653"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6842622.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            5413..5826
                     /locus_tag="VBC23_000654"
     CDS             5413..5826
                     /locus_tag="VBC23_000654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6842623.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            5891..6121
                     /locus_tag="VBC23_000655"
     CDS             5891..6121
                     /locus_tag="VBC23_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO6842624.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            6184..7455
                     /gene="murA"
                     /locus_tag="VBC23_000656"
     CDS             6184..7455
                     /gene="murA"
                     /locus_tag="VBC23_000656"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6842625.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            7457..7648
                     /locus_tag="VBC23_000657"
     CDS             7457..7648
                     /locus_tag="VBC23_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6842626.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            7723..8580
                     /locus_tag="VBC23_000658"
     CDS             7723..8580
                     /locus_tag="VBC23_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6842627.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            8871..9911
                     /gene="pheS"
                     /locus_tag="VBC23_000659"
     CDS             8871..9911
                     /gene="pheS"
                     /locus_tag="VBC23_000659"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6842628.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            9994..10514
                     /locus_tag="VBC23_000660"
                     /pseudo
     CDS             9994..10514
                     /locus_tag="VBC23_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            10568..12973
                     /locus_tag="VBC23_000661"
     CDS             10568..12973
                     /locus_tag="VBC23_000661"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6842629.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGGDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(13042..13944)
                     /locus_tag="VBC23_000662"
     CDS             complement(13042..13944)
                     /locus_tag="VBC23_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6842630.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            14122..17355
                     /gene="rexB"
                     /locus_tag="VBC23_000663"
     CDS             14122..17355
                     /gene="rexB"
                     /locus_tag="VBC23_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6842631.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGSKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            17345..20968
                     /gene="addA"
                     /locus_tag="VBC23_000664"
     CDS             17345..20968
                     /gene="addA"
                     /locus_tag="VBC23_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO6842632.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            20981..21898
                     /locus_tag="VBC23_000665"
     CDS             20981..21898
                     /locus_tag="VBC23_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6842633.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(21930..23306)
                     /gene="mnmE"
                     /locus_tag="VBC23_000666"
     CDS             complement(21930..23306)
                     /gene="mnmE"
                     /locus_tag="VBC23_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6842634.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            23434..25344
                     /locus_tag="VBC23_000667"
     CDS             23434..25344
                     /locus_tag="VBC23_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6842635.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            25494..26462
                     /locus_tag="VBC23_000668"
     CDS             25494..26462
                     /locus_tag="VBC23_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6842636.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFIQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            26537..27535
                     /locus_tag="VBC23_000669"
     CDS             26537..27535
                     /locus_tag="VBC23_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6842637.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            27662..29050
                     /locus_tag="VBC23_000670"
     CDS             27662..29050
                     /locus_tag="VBC23_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6842638.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            29110..30867
                     /gene="lpdA"
                     /locus_tag="VBC23_000671"
     CDS             29110..30867
                     /gene="lpdA"
                     /locus_tag="VBC23_000671"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6842639.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            30965..31954
                     /locus_tag="VBC23_000672"
     CDS             30965..31954
                     /locus_tag="VBC23_000672"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6842640.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT        10623 a         5250 c         6593 g         9957 t
ORIGIN      
        1 taacaactta ggattgatga catcattgaa aacacatgag cataattttt ggacatctcc
       61 gaccgctaat tttggtgtag atattacatt atcattatta gtagcattta tttgtcatat
      121 tgaaggtatt cgcaaaaaag gaattggtgg ttatctcaaa ggtttcttat caccaacacc
      181 agcaatgctt ccgatgaatt tattggaaga agttactaat gttgcttcat tagcattgcg
      241 tctttttggt aatatctttt ctggtgaagt tgtcactgga ttacttttac aacttgcagt
      301 tttatcacca tttacaggtc cactagcttt tgcattgaat atcgtttgga cagcattttc
      361 aatgtttatt ggtttcatcc aagcgtatgt gttcattatc ttgagctcaa gttatattgg
      421 acacaaagtt catggtgacg aagaagaata agaaaagaga ggagaaatat gtcaatactt
      481 attaatagta caacgattgg tgatatcatt atcgtatctg gctctgtgct tctcttattt
      541 atcttaatta agacttttgc atggaaacaa atcacaggta tttttgaagc ccgtgaacaa
      601 aagatagcta atgatattga tactgctgaa caggcacgtc aacaagctga agcttttgca
      661 actaagcgtg aggaagaact atctaacgct aaaactgaag ctaatcaaat cattgataat
      721 gctaaagaga caggtcttgc aaaaggtgat caaattattt cagaagcaaa aactgaagct
      781 gatcgattaa aagaaaaagc acatcaagat attgcccaaa ataaggcaga agcactagca
      841 gatgttaagg gtgaagtagc agatttaaca gttttattag ctgaaaaaat tatggtatca
      901 aatcttgata aagaagctca gagtaatctc attgatagct atattaaaaa gctaggagat
      961 gcctaatgaa taaaaagacg caggctctaa ttgagcaata ttcaaaaagc cttgttgaag
     1021 ttgccattga acataaaatt gttgaagaaa tccaacaaga agtagcagct ttaattgata
     1081 tttttgaaac aagcgagctt gagggtgtcc tgtcaagtct tgccgtttca catgatgaaa
     1141 aacaacattt tgtgaagaca ttgcaaacat catgttcaac ttatcttgtt aatttccttg
     1201 aggttattgt acaaaatgaa cgtgaagcac ttctatatcc tattttgaaa tcagttgatc
     1261 aggaacttat caaagtgaat ggtcaatatc caattcaaat tacaactgca gttgctttgt
     1321 ctccagaaca aaaagaacgt ctttttgata ttgctaaaac taaattagct ttaccaaatg
     1381 gacagttagt tgagcacatt gacccatcta ttgttggtgg atttgttgtc aatgcaaaca
     1441 ataaagtaat tgatgcaagt gttcgtaatc aattacatca atttaaaatg aaattaaaat
     1501 agaaagtggt gtgacttttg gcaattaatg cacaagaaat tagcgcttta attaaaaagc
     1561 aaattgaaga cttccagcca aattttgacg tcacagaaac tggtattgtt acatatatcg
     1621 gtgatggtat tgctcgtgct cgtgggctag ataatgctat gagtggagaa cttcttgaat
     1681 tttcaaatgg tgcgtacggt atggctcaaa accttgagtc taatgacgtg ggtatcatta
     1741 ttttgggtga tttctcagaa attcgtgaag gtgatgtggt aaaacgtact ggtaaaatta
     1801 tggaagtacc agtaggggaa gctatgattg gacgtgtcgt caatccgctt ggtcaaccag
     1861 ttgatggact aggtgaaatt gagacgacag caacacgccc agttgaaaca ccagcacctg
     1921 gtgttatgca acgtaaatca gtttttgagc ctcttcaaac aggtctcaaa gcgattgatg
     1981 ctttggttcc tatcggacgt gggcaacgcg aattgattat cggtgaccgc caaactggta
     2041 agacgtctgt agcgattgat gcaatcttga atcaaaaagg tcaagatatg atctgtatct
     2101 atgtggctat cggtcaaaaa gagtctacag tacgtacaca agttgaaact ttgcgtaaat
     2161 atggtgccct tgattacaca atcgttgtga cagcttcagc atcacaacca tctccattac
     2221 ttttcattgc tccatatgca ggagtagcaa tggctgagga attcatgtat aacgggaaac
     2281 acgttttgat tgtttatgat gatttatcaa aacaagccgt agcttatcgt gaactttcgc
     2341 ttcttcttcg ccgtcctcca ggtcgcgaag cttacccagg ggatgttttc tatcttcata
     2401 gtcgtctttt ggaacgttca gctaaagtat cagatgcttt aggtggaggt tcgattacag
     2461 cgcttccgtt cattgaaaca caagcaggtg atatctcagc atatattgct acaaacgtta
     2521 tttcaattac tgacggccaa attttcttgc aagaaaatct ctttaactca ggtattcgtc
     2581 cggcgattga tgcaggttca tcagtatctc gtgttggtgg tgcagcgcaa attaaagcga
     2641 tgaagagagt tgcaggtacg cttcgtctag atcttgcttc ttatcgcgaa cttgaggcat
     2701 tcactcaatt tggttcagac cttgatgcag caacacaagc aaaactaaat cgtggtcgtc
     2761 gcacagtgga agtgttaaaa caaccattac acaaaccatt gccagttgag aaacaagttg
     2821 tgattttata tgctctaaca catggattcc ttgatgatgt acctgtaaat gatattttag
     2881 cttttgaaga agctttgtat gattactttg atgctcatta tgataatctc ttcgaaacaa
     2941 tccgcacaac aaaagattta ccagaagaag cagaattaga tgctgctatt caagcattta
     3001 aagatcaatc acaatttaaa taataggagg gttctaatat ggcaggctct cttagtgaaa
     3061 ttaaagacaa gattctttct acagaaaaaa caagtaagat tactagtgcg atgcagatgg
     3121 tctcatctgc caagttggtt aaatctgaac aagctgctag agattttcaa gtgtatgcct
     3181 ctaaaattcg tcaaattact actaacctct tgaagtcaga cttagtatct ggatcagata
     3241 accctatgtt agcttcacgt ccggtgaaga agacaggtta tattgttatt acttctgata
     3301 agggtttagt tggtggttac aattcaaaaa tcttaaaagc gatgatggac actataactg
     3361 actatcatac tgagaatgat gattatgcca ttatttcaat tggtagtgtt ggttctgatt
     3421 ttttcaaagc acgtggaatg aatgtttctt tcgaattacg tggtttagag gaccaaccaa
     3481 gttttgatca agttggtaaa attattgctc aagcagttga aatgtacaaa aatgagttgt
     3541 tcgatgaatt atatgtttgc tacaatcacc atgttaacag tttaactagc caagtcagaa
     3601 tgcaacaaat gcttcctatc aaagaattgg atgcagaaga ggcttctgag gatagagtga
     3661 ttactggttt tgaacttgag ccaaaccgtg aagttatatt ggagcaatta ttgcctcaat
     3721 atactgagag tttgatttat ggagctatta ttgatgctaa aacagcagag catgcggctg
     3781 gtatgacagc tatgcagact gcaaccgata atgctaaaaa tgttatcaat gatttaacta
     3841 ttcaatataa ccgtgctcgt caagcagcta tcacacagga aattacagaa attgttgcag
     3901 gtgctaatgc acttgaataa ttaattagtc tatagaaaaa atagtgaaca aaggtatttt
     3961 aaatcctttg tatcatatga taaggagaaa tacatgagct caggcaaaat tgctcaggta
     4021 gttggacctg tcgttgacgt tgtatttgca agtggcgaca aacttcctga gattaataat
     4081 gcattgattg tctataaaaa tggcgataag tcacaaaaag tagtacttga agttgctctt
     4141 gaacttggtg acggcctcgt tcgtacaata gctatggaat caactgatgg gcttacacgt
     4201 ggtttggaag tattagatac tggtcgcgca attagtgtgc cggttggtaa ggatactttg
     4261 ggtcgtgtct tcaacgttct tggagatgct attgaccttg aagagccttt tgcagaagat
     4321 gcagaacgtc aaccaatcca taaaaaagca ccatcgtttg atgaattatc aacatcatca
     4381 gaaatcttag aaacaggtat taaagttatt gacttattag caccttactt aaaaggtggt
     4441 aaagtcgggc ttttcggtgg tgcgggtgtt ggtaaaaccg ttcttattca agaattaatc
     4501 cacaacatcg cccaagaaca tggtggtatt tcagtattta ctggtgtagg agaacgtact
     4561 cgtgaaggga atgaccttta ttgggaaatg aaagaatctg gcgttattga aaaaacggct
     4621 atggtctttg gtcaaatgaa tgaaccacca ggagcacgta tgcgtgtggc acttactggt
     4681 cttacaatag ctgaatactt ccgtgatgta gaaggacaag atgtgcttct cttcattgat
     4741 aatatcttcc gtttcacaca agcggggtca gaagtgtcag cgcttttagg tcgtatgcct
     4801 tcagccgttg gttatcaacc aacacttgct acagaaatgg gacaattaca agagcgtatc
     4861 acttcaacta aaaaaggttc tgttacatca atccaagcta tttatgtgcc agcagatgac
     4921 tatactgacc ctgcgccagc tacagccttt gctcacttag attcaacaac taaccttgaa
     4981 cgtaaattga cacaaatggg gatttatcca gccgttgacc cattagcttc aagttctcgt
     5041 gcactcacac cagaaatcgt tggagatgag cactatgaag ttgcaacaga agtacagcgt
     5101 gttcttcaac gttaccgtga gttacaagat atcattgcga ttttaggtat ggatgaatta
     5161 tcagatgagg aaaaaacgtt agttggacgt gcacgtcgta tccaattctt cctttctcaa
     5221 aacttcaatg ttgcagaaac atttactggt caaccaggtt catatgttcc ggttgaagaa
     5281 actgttcgcg gattcaaaga aatcctagat ggtaaacatg atcaaattcc agaagatgcc
     5341 ttccgtatgg ttggtggtat tgaggatgtt atcgcaaaag cagaaaaaat gaactattaa
     5401 tgaggtgatg ctatggcaca attgactgtt caagtagtaa catcagatgg tattagatat
     5461 gaccatcacg ctagtctcat tactgttaga acgccagatg gtgaaatggg aattttacca
     5521 ggtcacataa atttgattgc tccccttatt gttcatcaga tgaagattaa tcgttctcat
     5581 caagaaggtg tagactgggt agctgttaat ggtggtataa ttgaagttaa tgaggatcag
     5641 gtgacaattg ttgcggattc agcagaacga gcacgtgata tagaccttaa ccgtgcagaa
     5701 cgggctaagg aacgcgctga gcgtgcatta gaaaaagctc aaacaactca gaatattgat
     5761 gagatgcgta gagctgaggt tgctcttcgt cgcgcaatta accgtattag cgtcggtaaa
     5821 aagtagaaaa atggtctaat tagttttgtg actaattagg cttttttagt gtataataga
     5881 aggcatgaat atgatattaa atatagtaac tatagttagt aattttattt ttattgccct
     5941 tttttatcaa ctttttgtag atttatttga ctggtctaaa atgattaaaa tgtcccctca
     6001 aaacatctca aaattaaaag tttttatttt gttgataagt atagttggtg gctatgtggt
     6061 tagtcatttt ttattggaag tgattcagtt gtgtcaatca atcttttggg ctctccaata
     6121 aaggaaatat tagctgaact atgatataat caacttacta ttgttataag aatggagatt
     6181 caaatggata aaattattgt ggaaggtggg caaacgcggc ttcaaggtca agttgttatt
     6241 gaaggagcta aaaatgcagt tttacctctt ttagcagcca caattttacc aagccaaggg
     6301 aaaacattat tgaccaatgt tcctatcttg tctgatgtat ttacaatgaa caatgttgtt
     6361 cgtggtttag atattcaagt tgactttaat tgcgataaaa aagaaatctt agtggatgcc
     6421 tcaggtgata ttttagatgt tgcaccttat gaatttgtta gtcaaatgag agcttctatt
     6481 gttgttttgg gacctatttt agctagaaat ggccatgcta aggtttcaat gccaggcggt
     6541 tgtacaattg gtagccgacc aattgacctt catcttaaag gtttagaagc tatgggagct
     6601 acgattacac aaaatggtgg tgatattact gcccaagctg aaaagcttaa gggggctaat
     6661 atatacatgg attttccctc agtcggtgcc actcaaaact tgatgatggc tgctacactt
     6721 gcttcaggca caacaacgat agagaatgct gcacgtgaac cagaaattgt agaccttgcg
     6781 cagttactaa ataaaatggg agctaaagtc aagggtgctg gtacggaaac attaacgatc
     6841 attggtgttg atgctttaca tggtacagaa catgatgttg tccaagatcg tattgaagct
     6901 ggaaccttta tggtggcagc tgctatgact tctggaaatg ttctagtaaa ggatgctatt
     6961 tgggaacata accgtccttt aatctctaaa ttgatggaga tgggtgttga agtgtcagag
     7021 gaagaagatg gtattcgtgt aaaagctgat actaaaaaat tgaagccagt gactgtgaaa
     7081 acactccctc atccaggatt tcctactgat atgcaagctc aatttacagc tctaatggca
     7141 gtagttaatg gcgaatcaac tatgattgag acagtttttg aaaatcgttt tcaacatttg
     7201 gaagaaatgc gtcgcatggg attacaaacg gaaattttac gagatactgc aatgattcat
     7261 ggtgggagag ccttacaagg tgcaccggtc atgtctactg acttacgagc tagtgcagct
     7321 ttaatcttgg caggaatggt tgcgcaaggg caaacagtag ttggccaatt aacccattta
     7381 gaccgtggtt attatcaatt ccatgaaaaa ttagcggctc ttggtgctaa tattaaacgt
     7441 gtaagtgagg cgtaatatgg catctagtgg ttggaaatat gtactaaaac aaatcggtct
     7501 aatagtatta gtcatactgt tagccttgct gtttttagcg gttggattga tgttgggtta
     7561 tagtgttttt ggtgacggag agcatgctta ctctatttta tcgctggata aatggcaaaa
     7621 tattattgga aaatttttag gaaaatagaa agagccacta taagttattt gatgcttata
     7681 gtggtttcct ttgagagtta attttagcag tagaataata caatgacaaa aaaacaaaaa
     7741 caaataacag cttcgattgt aactttgctt ttttcattac tggcaatagg gttaggtgaa
     7801 agtaaatctt tgcctaatga tcacatttta aaacaggtga cagatttaat tattagtaat
     7861 gaatctggca aaaatacttc ccttaaaaat atatcaggaa cccctagtag agagttatct
     7921 gaatctgttt tgactagcaa tgttaaaaaa caattgggaa caaatatagc ttggaaccag
     7981 tcaggtgctt ttatcattaa tcaaaataaa acagatttga atgctaaagt gtcaagtgca
     8041 ccatatgcta ttaatgaaat aaagaaggtt aataaccaaa ttgttccaac taaagcaaat
     8101 gccttattaa caaaagcaac tcgtcaatat cgaaatcgag aagaaacagg gaatggtaga
     8161 acttattgga aaccagctgg atggcatcag ataaatggat taaaagggag ctataaccac
     8221 gctgtggaca gaggccattt aattggttat gctttagtag ggagtttaag aggatttgat
     8281 gcctccacaa gtaatcctaa aaatattgcc acacaagcag cttgggcaaa ccaagctaat
     8341 agcaaccaat caacagggca aaattattat gaaactttag ttcgtaaagc attggatcgt
     8401 cataaaactg ttaggtatcg tgtgacttta atttatgaca gagataatct tttatcttcg
     8461 ggatcacata ttgaggctaa atcttcagat ggaagtttag aatttaatgt ttttattcct
     8521 aatgtacaat cgggattatt atttgattat gcaacaggaa aagtgaagca gacaaagtaa
     8581 tctttatcta atagtgcttg ttaaagactg aaaaatttga taaaatagtc cttaattgaa
     8641 tagaggtctg tgagactagt aaaccaaggg aagctgctta gggagttcag ccgtgactgc
     8701 aagctgaatc agtgaaagag ggtggaattc actcacaaat aggactataa ttaaggtctg
     8761 attggtcgat cagatagaaa acggatggta ccgcgtgtca acgctccgat taagggggag
     8821 tggcacgctt tttttgtttt caaagctact tattttgact tggaggaaag atggatttac
     8881 aaaaacaatt agaagagttg aaaatatcaa ctcaagaaaa gcttaaagaa atgactggga
     8941 accacactaa agaattacaa gacttacgtg tacaagtttt aggaaaaaaa ggttctctaa
     9001 cagaattact aaaaggtctg aaagatttat ctaatgattt acgaccagtt gttggaaaac
     9061 aagtcaatga agtacgtgat attttaacaa aagcgtttga ggaacaagct aaagtagtgg
     9121 aagctgcgaa gattcaagca cagttagagt ctgagagtgt cgacgttacc ttgcctggtc
     9181 gtcaaatgac attaggtcac cgtcatgtct taacacaaac tagcgaagag attgaagata
     9241 tctttttagg tatgggattc caagtggttg atggttttga agttgagaaa gactactata
     9301 attttgaacg aatgaattta ccaaaagatc atccagcccg tgatatgcaa gatacttttt
     9361 atattacaga agaaatcttg ctacgtactc atacttcgcc tgttcaagca cgaacaatgg
     9421 accaacatga tttttctaaa gggcctttaa aaatgatttc tcctggacgt gttttccgtc
     9481 gtgatactga tgatgctact catagccatc aattccatca aattgaaggc ttagttgttg
     9541 gagaaaatat ttcaatgggc gatctcaaag gaacacttca actgatttct caaaaaatgt
     9601 ttggagcaga gcgtaaaatc cgacttcgtc cttcatattt cccattcaca gaaccttcag
     9661 ttgaagttga tgtctcttgc ttcaagtgcg gaggtaaggg atgtaatgtc tgcaaacaga
     9721 caggttggat tgagatattg ggtgctggta tggttcaccc aagtgttttg gaaatgtctg
     9781 gtattgattc tgagaaatac tccggttttg cctttggctt aggtcaagaa cgtattgcaa
     9841 tgttacgcta cggtatcaat gacattcgtg gcttctatca aggtgatgtt cgcttcacag
     9901 atcaatttta agattttaga tagcaagtag catcattggt gtgatgataa atttatgtta
     9961 acgctattac cttactcaat aggaggtttt ctaatgtctc aaattacttt taaaaaggtt
    10021 ggccttgata atgtgaatat tttacagaat cttgctatag aaacctttcg gcagacgttt
    10081 tctcatgata atagtgagga acaacttcaa gcatttttca atgagtctta cactttacca
    10141 gtattaaaat cggaaattac ccatgctgaa tctgatactt attttgttta tcttgataca
    10201 gatttagtgg gttatttaaa agtaaactgg ggaagtcagc aaactgaaaa aaacttgata
    10261 aagcatttga aattcaaagg atttatcttc tagatgccta ccaagggaaa ggtataggta
    10321 aggctacttt cgaatttgct ttggatttag cttataaatc aggcttagat tgggcttggt
    10381 taggtgtttg ggagtttaat cataaagctc aggcatttta cgctaaatac ggatttgaaa
    10441 aattttctga gcaccaattt tcagtaggtg ataaagtgga tacggattgg ttattaagaa
    10501 aaagtttaca ctaaaaacta caaagcactt gtataaaaat aaaagaaaat aaatagagag
    10561 aaattacatg ttagtatcgt ataaatggtt aaaagagtta gtggatgttg atgtcacaac
    10621 agcagaacta gctgagaaaa tgtcaaccac tggtattgag gttgaaggtg tggagacacc
    10681 agcggaaggt ttatcaaaat tagttgtagg tcatattgtt tcgtgtgaag atgttccaga
    10741 tacccactta catttgtgtc aagttgatac agggggcgat gaacttcgtc aagttgtctg
    10801 tggtgctcca aatgtcaaga ctggtattaa tgttatcgta gcggttcctg gtgctcgtat
    10861 tgcagataac tataagatta aaaaaggtaa aatccgtggt atggagtcac ttggaatgat
    10921 ttgttcactt caagagttgg gcttatcgga aagtatcatt ccaaaagagt tttctgatgg
    10981 tatccaaatt ttacctgagg gagctatacc tggtgatagt attttctctt atcttgactt
    11041 agatgatgaa attattgaat tatcaattac accaaaccgt gcagatgctc tttcaatgcg
    11101 tggtgttgcc catgaggtag cagcaattta tgggaaaaaa gttcattttg aagaaaaaaa
    11161 tctcattgaa gaagcagaaa gagcagctga taagatttct gttgtcattg aatcagataa
    11221 agttttatct tactcggctc gtattgttaa gaatgtaaca gttgcaccaa gtccacaatg
    11281 gcttcaaaat aaactgatga atgctggtat acgtccaatt aataatgttg ttgatgtgac
    11341 aaactatgtt cttttgactt atggtcaacc aatgcatgct tttgattttg ataaatttga
    11401 tgggacaact atagtagcac gtaatgctga gaatggtgaa aagttaatta ctcttgatgg
    11461 tgaagagcgt gacttaatag ctgatgactt ggtcattgcg gtaaatgatc aaccagttgc
    11521 tttggcaggc gttatgggag gacaatcaac tgaaattgat agttcttcta agacagtggt
    11581 ccttgaagcg gcagtattta atggaacaag tatccgcaaa acaagtggaa gactcaacct
    11641 tcgttcagag tcatcttctc gctttgaaaa aggtatcaat tatgatacgg tttcagaggc
    11701 tatggatttt gcggctgcaa tgttacaaga attggcaggc agtcaagttc tttctgggca
    11761 agttacagaa ggagttcttc caactgagcc ggtagaggta tcaacgactc ttggttacgt
    11821 taatactcgt ttgggaacag aacttaccta tactgatatt gaagaagtat ttgaaaaact
    11881 cggctttgct atttctggtt cagaagtgaa atttactgtg ttggtaccgc gtcgtagatg
    11941 ggatattgcc attcaagctg acttagttga agagattgcc cgtatttatg gttacgaaaa
    12001 attaccaaca acattgccag aagccggagc gactgcagga gaattaacga gtatgcaacg
    12061 cttacgtcgt agagtccgta cggttgctga aggagtagga ttatctgaaa ttatcaccta
    12121 tgctttaacg actccagaaa aagcagtgca gttctcaact caagcaacta atataactga
    12181 acttatgtgg ccgatgactg ttgatcgctc agcacttcgc caaaatgttg tttcaggaat
    12241 gcttgataca attgcttata acgttgctcg caaaaacagt aaccttgcag tttatgagat
    12301 aggaaaagtt tttgaacaaa ctggtaatcc aaaagaagat ttaccaactg aagtagagac
    12361 gtttactttt gccttaacag gtctagtaga ggaaaaagat ttccaaacaa aagccaagcc
    12421 agttgatttc ttctacgcca aaggtattgt agaggcgctc tttattaaat taaaattaga
    12481 tgttactttt gtagcacaaa aaggtttagc aagtatgcac ccaggtcgta cagcaactat
    12541 cttattagat gggaaagaga ttggttttgt tggtcaagta cacccacaaa ctgcaaaaca
    12601 atatgatatt cctgaaactt atgttgctga aatcaatcta tcaactattg aaagtcaaat
    12661 gaaccaagcg ttgatttttg aagatattac gaagtatccg tctgtatctc gcgatattgc
    12721 attattactt gcagaatctg ttagtcatca tgatattgtg ttagcaattg agtcatcagg
    12781 ggtaaaacgt ctaacagcga ttaaactgtt tgatgtctac gctggaaata atattgcaga
    12841 agggtataaa tcaatggctt atagcttaac tttccaaaac cctaatgaca atctaacgga
    12901 cgaagaagtg gctaaatata tggaaaaaat cactaaatca ctcgttgaga aagttaatgc
    12961 agaaataaga taatttatat cttacgttga aaaataaata aaaaacagca tcaattaaga
    13021 tgctgttttt ttatttaaaa tttatagatg ttctgcggag aaagtatcac cgtgttggat
    13081 gtcaccatat tgaaagcctt tgttaaacca gcgttggcgt tgttgagctg ttccatgggt
    13141 aaagctatca ggcactaatt ttccgtaggt ttctttctga agggtatcgt ctccgacggc
    13201 gtgggcagca ttcatggcct cttcaaagtc tccttgttct aagagatttt ttcccctgat
    13261 gtagtgagcc catacccctg cataataatc tgcttgaagt tctagccgaa catttaaagc
    13321 atttgcttct ttcttagtaa gtccgtgtcg cattctatta tacttatcca taatgcctaa
    13381 ctctgtttga atgtggtgac caacttcatg ggcgatgacg taggccatag caaaatcacc
    13441 agtagcacca tatttatgtg ataattcatt gtaaaaagaa atatcaagat agattttttt
    13501 atctgctgaa caataaaatg gttctgaagc agattcacct ataccacaac ctgtttgaat
    13561 cgaattggtg taaagaacaa gttttggttc cttataattt ccaaaacctt gggtttggaa
    13621 ttcttgtgac cagaaatcct cagttgatcc aaggacttta ttaacgaaat cgatttgttc
    13681 tctcgttgcg ctattatcaa cagaacgtga gacattctga gattggtaac tagaaggtga
    13741 ggatgagtca ttaaaaatgc tggttagtcc ccctccgcca agtagcagta agatgattaa
    13801 aaccacaagc ttggttttcc aactccctcg catcaataaa agttgaagaa tcggtaatcc
    13861 acttcctccg ctagagaatg aacctccgct actggagcga cgatcttcaa cattgtcgct
    13921 ttttcttagg tcatctattt tcataagtat ctccaaagtg ttttataggc tcattataaa
    13981 ctattttaat aagaaatgct tgaattttta actaaatatc ttcccctaga tttgaagcta
    14041 tttttggtat aatattaata aactgtaaag aataagttga agaaggttta tttctcagtt
    14101 gacttataag ataggagatt catgaaatta ctttatactg atattaatca tgatatgaca
    14161 gaaatcttag tcaatcaggc cgcgcatgct gctgaagctg gatggcgtat tttttatatt
    14221 gctccaaact ctttatcttt tgaaaaggag cgtgcagtat tagagaccct tcctcaagaa
    14281 gcctcttttg ctattacaat tactcgcttt gctcaattgg cacgttattt tactttaaac
    14341 cagcctaatc aaaaagaaag tttaaacgat attggattag caatgatctt ttatcgtgct
    14401 ttagcaagtt ttgaagatgg acaattaaaa gtttttggac gattgaagca agatgcttcc
    14461 tttatcagtc aattggtaga cctatacaaa gaattgcaga cagcaaatct gagtatttta
    14521 gatttaaaat atttacattc tcctgaaaaa tttgaagatc tattagctat ttttctagtg
    14581 gtttcagatc ttttaagaga aggagaatac gacaatcaat ctaaaattgc cttttttaca
    14641 gaacaagtaa ggtcaggaca gctagatgtg gatttaaaga acactatcct tatagtcgat
    14701 ggttttaccc gtttctcagc tgaagaagaa gctttaatta aaagtctgag ttcacgttgt
    14761 caagagatta tcatcggagc ttatgctagc caaaaagcgt ataagactaa ctttaccaat
    14821 ggaaatattt attctgcagg ggttgatttt ttaagatatc tagcaacgac atttcaaaca
    14881 aaaccagaat ttatcttgtc taagtgggaa agtaagtctg gttttgaaat gatttcgaaa
    14941 aatatcgaag gtaaacatga ttttacaaat agtagtcaca ttttagacga cactgctaaa
    15001 gattgtataa caatttggga gtgcattaat caaaaagatg aagtggaaca tgtggctaga
    15061 gctatacgtc agaaacttta tcagggatat cgctacaaag atattttagt tttactagga
    15121 gatgtagata gttataaact acaattaagt aagatttttg aacagtatga tattccttat
    15181 tatttcggta aagctgaaac aatggctgcg caccctttag ttcattttat ggattccttg
    15241 tcgcgaataa aacgttatcg atttagagct gaagatgttt taaatctttt taagacaggt
    15301 atttatggag agattggtca agatgattta gattattttg aagcttatat ctcatatgca
    15361 gatattaaag gttctaagaa gttctttaca gattttgttg tgggggctaa aaaatttgat
    15421 ttagggcgtc ttaacactat tcgtcagtcc ctccttactc cactggaaag ttttgttaaa
    15481 acgaaaaaac aagacggtat aaaaacactc aaccaattca tgtttttcct tactcaggta
    15541 ggtttgagtg acaatttatc gcgattagtt ggtcaaatgt ctgaaaatga acaagaaaaa
    15601 catcaagaag tttggaaaac atttacagat attttagagc agtttcaaac aatatttggt
    15661 caagaaaaat taaatttaga cgaattttta tcgttattaa attcaggtat gatgcaagcc
    15721 gagtatcgta tggtccctgc tactgttgat gtggttactg ttaagtctta cgacttggta
    15781 gaaccacata gtaatcaatt tgtctatgcc ctaggcatga cacaatcaca ttttccaaag
    15841 attgctcaaa acaaatcttt aatatctgat attgaacgtc agcttataaa tgatgccaat
    15901 gacactgatg gtcattttga cattatgacg cgagaaaatt tgaagaaaaa ccattttgct
    15961 gctctctctc tttttaatgc tgctaaacaa gcattagtgt tgactatacc acaattattg
    16021 aatgagtctg aagatcagat gtcaccatat cttgtcgaac taagagatat aggtgttcct
    16081 tttaatcata aaggacgcca atcgttaaaa gaagaagctg ataatattgg gaattacaaa
    16141 gctttattat cgcgtgttgt tgacttgtat cgctctgcta ttgacaaaga gatgactaaa
    16201 gaagaacaaa ctttttggtc agttgctgtt cgttatttgc gtcgtcaatt aacttctaaa
    16261 gggatcgaga taccaataat tacagatagt cttgatactg tgacagtttc atcagatgtg
    16321 atgaccagac gcttcccaga agatcaccca cttaaattat cctcatctgc tttgacaact
    16381 ttttataata atcaatataa gtatttttta caatatgtgt taggtttgga agaacaagac
    16441 tcaattcatc cagatatgcg gcaccacgga acgtatcttc atcgtgtttt tgaaattctg
    16501 atgaaaaacc aagggataga atcttttgaa gagaaactca actctgcaat taataaaacc
    16561 aatcaagaag atgttttcaa atccttgtat tcagaagatg cagaaagtcg ttattcttta
    16621 gaaattttag aagatatcgc gcgtgcaact gcaaccatat tacgacaaga tagtcagatg
    16681 actgttgaaa gtgaagaaga acgatttgaa ctaatgattg ataatactat aaaaattaac
    16741 ggtattattg accgtatcga tcgtttatct gatggttctt tgggtgtcgt ggactataaa
    16801 tcaagcgctc aaaaattcga tatccaaaaa ttttataacg gtctaagtcc acaattagtc
    16861 acctacattg atgctatcag tcgtgacaag gaagttgaac aaaaaccacc gatttttgga
    16921 gctatgtact tacatatgca agaacctaga caagacttgt ctaaaattaa aaatttagat
    16981 gatttagtaa caaaaaatca tcaagcttta acttataaag gattattttc agaagccgaa
    17041 aaagaattct tagcaaatgg gaaataccat ctcaaagatt ccctttattc agaggctgaa
    17101 attgctattt tacaagcaca taatcaatcg ctttacaaaa aagcatctga gactattaag
    17161 tcaggtaagt ttttgatcaa tccatataca gaagatgcta aaacagttga cggtgatcag
    17221 tttaagagta ttacggggtt cgaggcggat aggcatatgg cacgtgccag agcgctttat
    17281 aaattacctg ctaaggaaaa acgtcaaggt ttcttaacat taatgcagca agaggaggaa
    17341 aatgatgacc tttaagcctt tcttaaatcc agaagatatt gctgttattc agacagaaga
    17401 aaaaaattct gataaaaaac aaaagcgaac gcctgaacaa attgaagcaa tttatacttt
    17461 tggtaacaat gtcctggtat cagcttcggc tggttctgga aagacttttg taatggttga
    17521 gcggatttta gataaacttt taagaggggt tccgattgag tctttgttta tctcaacatt
    17581 tacagtaaaa gcggcaggtg agctcaaaga acgtttagaa aagaaaataa acgagtcttt
    17641 gaaatccgca gaatcagatg atttaaaaca gtttctcacc cagcaacttg tcggcattca
    17701 aacagctgat attggtacta tggatgcttt tacgcaaaaa atagttaacc aatatggtta
    17761 tactttgggc atatcaccta tatttaggat tttacaagat aaaaatgagc aagacgtcat
    17821 aaagaatgag gtatatgctg atttattttc tgactacatg actgggaaga atgcagcttc
    17881 attcataaaa ttagtcaaaa atttctcagg taatcgtaaa gatagcaaag cttttcgaga
    17941 aatggtttat aaagtttatg cttttagcca gtccatcgat aaccccaagc gctggatgca
    18001 aacagttttt ctaaaaggtg cacaaactta cactgatttt gaagctattc cagatcaaga
    18061 agtttcaagc cttttaaatg taatgcaaac aactgctaat caattacgtg atttaacgga
    18121 tcaagaagat tataagcaat taactgctaa aggggttccg acagcaaatt acaaaaaaca
    18181 ccttaaaata attgaaaatt tagtccattg gtctcaagac tttaatttgc tgtatggaaa
    18241 aaaaggattg gctaatttgg cacgtgatat cactaatgtg attccttcag gcaatgatgt
    18301 tacagttgct ggcgttaaat atcctatctt taaacaattg cataatcgta tcgttggact
    18361 taagcactta gaagttattt ttaagtatca aggagaatct cttttcttgt tagaattatt
    18421 gcaatccttt gttttagatt tctcagaaca atatttacag gagaagattc aagaaaatgc
    18481 ttttgaattt tctgatattg cccattttgc aattcaaata ttggaagaaa atcatgatat
    18541 taggcaaatt tatcaagata agtatcatga agtaatggtt gatgagtatc aggacaataa
    18601 ccatacccaa gagagaatat tagaattatt gtctaatgga cataatcgtt tcatggttgg
    18661 agacataaaa caatcaattt accgttttcg tcaggctgat ccacaaattt ttaatgataa
    18721 atacaaagct taccaagaca atccaagtca agggaaatta attattctga aagagaattt
    18781 tcggagtcaa tcagaggttt tagacagtac aaatagtgtg tttacacatc ttatggatga
    18841 agaagtagga gacattcttt atgatgaaag tcaccaatta aaagctggta gtccaagaca
    18901 gcaagaaaga catccgaata ataaaacaca agttttacta ttagatactg atgaagatga
    18961 tatagatgac agtgatagtc aacaatatga tattagccca gctgaagcaa agttagtcgc
    19021 aaaagaaatt attcgattgc ataaagaaga gaatgttccc tttcaagata tcaccctctt
    19081 agtttcttca aggacaagga atgatggtat cttacaaact tttgaccgtt atggtattcc
    19141 tttggtaact gacggtgggg agcaaaatta cttaaaatca gtcgaagtga tggtaatgct
    19201 tgatactttg cgatcaattg ataatccctt aaatgattat gctctagttg ctcttttgag
    19261 atctcccatg tttggcttta atgaagatga tttaacaaga attgctattc atgatgtcaa
    19321 aatggctttt tatcataaag tgaaattgtc ttatcataaa gaaggtcatc atagtgattt
    19381 aattactcca gaattatctt ctaaaataga ttactttatg aagacattcc agacttggcg
    19441 tgattttgct aaatggcatt cgctttatga tttaatatgg aaaatatata atgaccgttt
    19501 ttattatgat tatgtaggag ctctgcctaa agctgagcaa cgtcaagcca atctatatgc
    19561 cttggcactt cgagcaaatc agtttgaaaa aacaggtttc aagggtttat cacgttttat
    19621 tcgaatgatt gataaagttt tagaaaatga aaatgatttg gcagatgttg aggtggcatt
    19681 accgcaaaat gctgttaacc taatgactat acacaaatca aagggtcttg agtttaagta
    19741 tgtatttatt ttaaatattg ataagaaatt ttcaatggta gacataactt cccctcttat
    19801 tttaagccga aatcaaggaa ttggtattaa atatgttgct gatatgcgac atgaactcga
    19861 agaagagata ctgcctgcag taaaggttag catggaaaca cttccttacc aacttaataa
    19921 aagagagtta cgcttagcta ctttatcaga gcaaatgcgt ttactttacg tagctatgac
    19981 acgtgcagag aaaaaattat atctggttgg taaggctagt caaaccaaat gggctgatca
    20041 ttatgactta gtatctgaaa ataattatct cccattagct agtcgtgaga cttttgtgac
    20101 ctttcaagat tggttactgg ctgttcatga aacctataag aaacaagagt tgttttatga
    20161 catcaacttc gtatcattag aggaattaac agatcaccac atagggatgg ttaatcctag
    20221 tctacctttc aatcccgata ataaggcaga gaatcgtcag tctgaagata tcgtgcacgc
    20281 gattagcgtg ttagaatctg ttgaacagat taatcaaact tataaagcgg ctattgagtt
    20341 accgacagta agaacaccaa gtcaagtcaa aaaattctat gagcctattc tagatataga
    20401 aggcgttgat gtgatggaaa ctattactaa aacatcagta gattttaagt tacctgattt
    20461 ttctacaagt aaaaagcaag acccagcagc tttaggatct gctgtacatg aattaatgca
    20521 acgcatagag atgtcatctc atgtcaaaat ggaagatatt caaaaagcct tgacagaagt
    20581 gaatgctgaa acaagtatca aagcggctat tcagattgaa aaaatcaact attttttcca
    20641 agaaaactca ctcggtaaat atatacaaga agaagtagaa catctacatc gagaggcacc
    20701 ttttgccatg ttaaaagaag atccggagag tggtgagaaa ttcgttgtac gtggtattat
    20761 tgatgggtat ctactattag aaaatcggat aattctattt gattataaga ctgataaatt
    20821 tgtcaatcct ctggaattaa aagaacgcta tcaggggcaa atggctttat atgcagaagc
    20881 tttaaaaaaa tcttacgaga tagaaaggat agacaaatac ttaattcttt taggtggtaa
    20941 acagctagaa gtggtaaaaa tggattaaag aaaggaaaaa atgatagtcg aacaaaaatt
    21001 tggaaatggt tttacttgga ttaacattga agcagagcaa ttgcgaacag aaacatcgga
    21061 aattcaagca aaatatttgg atagtgagat tattacatat gctctggatg attatgaacg
    21121 agcctttatg gaatgttctc atattaaagg aaaagaagtc ttgacgatta tttttaatac
    21181 tattgatcta aagcagaaag aaagttatta tgagaccgtt ccgatgacct tttgcttatc
    21241 acacgaccgt ttgataacgg tgacacggtc tcgaaatagt tatatgctag aactattgca
    21301 aaaatatcta gaccgtaatc cagatgtctc tcctaaaaaa tttttatttg cagctttaac
    21361 tttgattaca aaacaatatt ttaatgttgt atcgaaaatt gatagagaaa aggatattct
    21421 caaccgtcaa ttgagagaac aaacaaccaa taagcgctta ttagccatga gtgacctaga
    21481 gacaggctcc gtttatcttt tgacagctgc caatcaaaat gcattggtat tagagcaatt
    21541 agatgttcat cctagtcagc gatttaattc tgaagtggaa aaagaacagt tatcagatgc
    21601 tctcattgaa gcgcatcagt tagtcagtat gacacaatta aacagtcagg tactttcaca
    21661 attatcatct acttttaata atgtcttaaa taataacttg aacgaaaact taacaggttt
    21721 gaatatcatt tcgattaatt tagcgattat tgcagcaatt actggctttt ttgggatgaa
    21781 tattccatta cctcttacag aatcacggag ttcatggtta attgttattg ctacttcggt
    21841 cttactttgg gttattattg ctcaaatttt aaaacgagtt tttaacttaa gaaaataaaa
    21901 atgttcagtg cgtaagcacc gaacattttt tatttaccta agcagaattg gctgaagagc
    21961 tgagttatta gctcatctgg agctgcatcc ccagtgattt ctccaagtat ttcccaagtt
    22021 ctagtcatat cgacttgtaa gagatcaaca ggcattccta attccaagcc ctcattgaca
    22081 gcttttaaac tatcaacggc tttttcaatt agtgagatat ggcgagcatt cgatagataa
    22141 gtagcgtcat gttctaccat accagcattg tcaaagaaaa tatcgttaat acgttcttcg
    22201 attttatcga tgttttcatt ttctaaaacc gaaattggaa taacattttc aggtaattcg
    22261 ttaacttcta ttttttgagg aagatccgtt ttattgagta atacgatacg attactttct
    22321 ttacttagtt ctagaaggga gcggtcttgt aatgttagtg gttcggaact attgagaacc
    22381 aacaacacaa ggtcggcttc ttctaaagcc ttctttgaac gttcaacacc aattttttca
    22441 acgatatcat ctgtatcacg gataccagca gtgtcaacta gttttaaagg aactccttta
    22501 atattgacgt attcttcaat aacatcacga gttgttcctt caatatctgt cacaatcgcc
    22561 ttttcttcac gtaagaggtt atttagtaag ctggatttac cgacatttgg acgaccaatg
    22621 attgctgttg aaagtccttc tcgaagaatt tttccacgtc gggcagttcg aagtaaattt
    22681 tccataagtg cttgaaactc ttgtgttttt tcacgcatca gtgttgtagt catctcttca
    22741 acgtcatcat attcagggta atcgatatta acttcaactt gggctagagt atttagaatt
    22801 tcttgtcgag tgttattgat tagtgtcttt aaagaaccat ctaattgctt aacggcaata
    22861 tccattgctt tatctgtttt agctctaata agatccataa cagcttcagc ctgagtcaag
    22921 tcaactcgac cgtttaaaaa agcacgttta gtaaattctc ctggttcagc cattcgtgct
    22981 ccatgtcgta aaattaactg aagaatttcg ttagtaactg caattcctcc atgcgtatta
    23041 atttcaataa catcttctct tgtgaatgtt tttggagcaa gcataacgcc taacataact
    23101 tcatccaaga tttcattttt atctgggtcc acaatatgac catagtttaa ggtatgggat
    23161 tggatagcgg atagatcttt tccacgataa atttttgatg ctatctttaa ggcatctgta
    23221 ccagatatac ggacaatacc aatagctccc tcaccgagag gagttgaaat agctgcgatg
    23281 gtatcaaatt cttttgtaat agacataaag tctctctctt tcttgggaat tactaacatt
    23341 cattgtaacc taaagcccta aaaggtgcaa tcatttgatt aaaagagaag atttccataa
    23401 tggttttatt tgaaggtatg ctataataaa actatgatta tactacaagg aaacaaaatt
    23461 gagcgctcat tttcaggtga tgtgcttttt gataatatta atattcaagt tgatcaacgt
    23521 gatcgtattg ctcttgttgg acgtaatggt gctggcaaat caacgcttct taaaatttta
    23581 gttggtgaag aggctccgac aaaaggagaa ataaataaaa agcgtgattt gagtttgtcc
    23641 tacttggcac aagatagtcg ttttcaatct gaaaatacaa ttttccaaga aatgttacaa
    23701 gtttttgatt cattaagaga agtagagaag cgcttacgtg aattggaatt acaaatggga
    23761 caagtttcag gaagtgacct ggaacaattg atgaaaacat acgatatttt gtcagaagag
    23821 tttcgtgaaa aaggtggctt tacctatgaa tcagatataa aggctatttt aaatggcttt
    23881 aagtttaaca gtgatatgtg ggaaatgcct attagtgaat tgtctggtgg gcaaaataca
    23941 cgattagcgc ttgcaaaaat gctacttgaa aagccagaat tacttgtttt agatgaacca
    24001 acgaaccatt tagacattga tacgattgct tggttagaaa attatcttgt aaattatcaa
    24061 ggagccctta ttattgtcag ccatgaccgc tattttttag acaaggtagc tacagtaact
    24121 tatgatttaa cgacacattc tctagaccgc tatgtaggca attattcgaa atttatggat
    24181 cttaaggcag agaaaatagc gactgaagaa aagaattttg aaaagcaaca aaaagaaatt
    24241 gctaagttag aagattttgt acaacgtaac attgttaggg cttcaaccac taaacgagca
    24301 caagctcgtc gtaaacaatt agaaaagatg gaacgactcg acaaaccgaa tgtagagcaa
    24361 aaatcagcta atatgacttt tcatgctggg aaagtctcag ggaatgttgt tttaacactt
    24421 gagaatgcag ctattggtta tgaaggtgtt tctttatcgg agccaatcga tttggacgtg
    24481 aagaaatttg atgctattgc aattgtaggt ccaaatggta tcgggaaatc aactttaatc
    24541 aaatcgcttg ttgggcaaat tccttttatc aaaggagaag caaaattagg agcaaatgtc
    24601 gaaactgggt attacgatca aagtcaaagt aatttaacaa aaacgaatac tgttttagat
    24661 gagttatgga atgctttttc aacaactcca gaagttgaaa ttagaaaccg tttaggagct
    24721 tttctttttt caggtgatga tgttaaaaaa tcagtttcaa tgctatccgg tggtgaacga
    24781 gcgcgtttgc tattggcaaa attatctatg gaaaataata atttcctcat attagatgag
    24841 ccaacaaacc acttggatat tgatagtaaa gaagtcttag aaaatgcact catcgaattt
    24901 gatggcacat tactttttgt tagtcatgat cgctatttta ttaatcgtgt ggcaactaag
    24961 gttctcgaaa tttctgataa gggaagtact ctctatctag gagattatga ttattacctc
    25021 actaagaaag ccgaactaga ggaattagcc cgtttaaatg aagaagaagt gagtgcttca
    25081 aaaacagaaa tcgacgttac atcagattat gagacacaaa aagcaaatca aaaggaattt
    25141 aggaaaatta cccgtcgcgt tgcagaaatt gaggcacgtt tggaagttct tgaaaatgat
    25201 gaaaacaata tcaatggttt aatgttagaa accaatgaca tcggaaaact ctctgattta
    25261 caaaaagagc tggaatcaat ccaagaagaa caattgctat taatggaaga atgggaaaat
    25321 ctaaatatga gactagattt gtgagaaaat ttcaaaaaca atagtttgtg aaaaaaagta
    25381 cattttaaat tgagatgcct tttacgaaaa caaagaaagc ggttcaaatt cttgcatctt
    25441 gtgaaaaaaa cttatacaat gattaagtaa taaagatttt tttggaggtt agaatggtaa
    25501 ctctatctaa agaacaacac ttggatatgt ttctaaaaat gcaacgtatt cgtgatgttg
    25561 atatgaaatt taacaaattg gttcgtcgtg gtttcatcca agggatgact cacttctctg
    25621 ttggtgaaga agctgcttca gtaggtgcta tccaagattt aacagattca gatatcattt
    25681 tctcaaacca ccgtggacac ggacaaacta ttgcaaaagg tattgatatt ggtggaatgt
    25741 ttgctgaact tgctggtaaa gcaacaggaa catcaaaagg tcgtggaggc tcaatgcact
    25801 tagcaaacct tgaaaaaggt aactatggaa caaacggtat cgtcggtgga ggatatgcgc
    25861 ttgcggtagg tgctgccctt acacagcaat atgaaggaac tgataacatt gttatcgcct
    25921 tttcgggtga ctcagcgact aacgaaggct cattccatga gtcagttaac ttagctgctg
    25981 tttggaactt accagttatc ttctttatca tcaacaaccg ttatggtatc tcaacagaca
    26041 tcacatactc aactaaaatt cctcaccttt acatgcgtgc tgatgcgtac ggtattccag
    26101 ggcattatgt tgaagatggt aatgatctta tggctgttta cgagaaaatg catgaagtta
    26161 tcaattacgt gcgttcagga aatggaccag ctattgtaga ggttgaatca taccgttggt
    26221 ttggtcattc aactgctgat gctggggtat accgtactaa agaagaagtt gattcatgga
    26281 aagctaaaga tccagtaaaa cgctaccgtg catacttaat tgaaaatgaa atcgctactg
    26341 aagaagagct tgcagctatt gaagcacaag tgattaaaga agttgaagaa ggggttaaat
    26401 tcgctgaaga aagtccattc cctgatatgt ctgttgcatt tgaagacgtt tttgtggact
    26461 aaaataacct aaagtagatg agatttatct cgttctatta ttcaattgat taaaaaacgg
    26521 atattcggag gaaataatga gcgaaactaa agttatggct ttgcgcgaag cgattaatgt
    26581 cgctatgagc gaagaaatgc gcaaagatga aaaagtattt ttaatgggtg aagatgtagg
    26641 tgtatatgga ggagacttcg gtacatctgt tggtatgtta gaagagtttg gtgctaagcg
    26701 cgttcgcgac acaccaatct cagaggctgc gattgcaggt tcagctatcg gtgctgcaca
    26761 aacaggtttg cgtcctatcg ttgatttgac attcatggat ttcgtaacaa tcgcaatgga
    26821 tgctatcgtt aaccaaggtg ctaaaacgaa ttatatgttt ggtggaggtc tatcaactcc
    26881 agttactttc cgtgtggcat cagggtcagg tattggttcg gctgcacaac actcacaatc
    26941 attagaagct tggttaacac acattccagg tctaaaagtt gttgcacctg gtactgttaa
    27001 tgagtcaaaa gcacttttga aatcttctat tcttgacaat aacccagtta tcttcttaga
    27061 gccaaaagct ctctatggta agaaagaaga agttaatatg gatcctgatt tctatattcc
    27121 acttggtaaa ggggatatta agcgtgaagg tactgactta acaattgttt cttatggccg
    27181 tatgcttgag cgtgttatgc aagctgctga agaagttgct gaggaaggta ttaacgttga
    27241 ggttgttgat ccacgtacac ttattccgct tgataaagaa ttaattatcg attctgttaa
    27301 gaaaactggt aaattgatct tggttaacga tgcttataaa actggtggtt tcactggtga
    27361 aattgcaact atggttgctg aaagtgaagc atttgattac cttgatcatc ctatcgtgcg
    27421 tcttgcaagt gaagatgttc cagtaccgta ttcacgtgta cttgaacaag gaatcttacc
    27481 tgatgtagca aaaattaaag atgctatcta caaagtagtt aacaaaggta aataattcat
    27541 aaaacaaaga gggactgagg aatgagtgaa aacttagcct ctttccttgt ttataggttt
    27601 taatttatat cgttagatga aacatatatt tgacgatata gtttagtgtg aaaggaaaac
    27661 tatggctgtc gaaattatta tgcctaaact cggggttgac atgcaagaag gcgaaatcct
    27721 tgagtggaaa aaacaggtag gtgatgttgt taatgaaggt gacgtcctac ttgaaattat
    27781 gtcagataaa acaaatatgg agattgaagc agaagactca ggtgttttac taaaaatcac
    27841 acatgggaat ggagatgttg ttcctgtaac tgagacaatt ggctgtatcg gtgctgaagg
    27901 tgaagaagta acagaagcat cttcttctga aaatacatca gttgaagaaa acgcgacaca
    27961 agtaacttca gaaccagaaa aagttgaaga aacatcagaa ccaagtgtgc cagcagctac
    28021 aagtggtgaa aaagtacgtg ccactcctgc agctcgtaaa ttagcaagag agatgtctat
    28081 tgaccttgca ctagtatctg gaacaggggc aaatggtcgc gttcacagag aagatgttga
    28141 aagcttcaag ggtgctcaac cacgtattac accattggca cgtcgaattg ctgaagatca
    28201 aggagttgat atagcagaaa tcactggcag tggtattcgt ggtaaaattg ttaaaaatga
    28261 tgttttggct gctatgtcac cgcaagcagc agaggctcct gtaaaaacga aagctacacc
    28321 aacaacagaa gaaaaacaat taccagaagg tgttgaagtc attaagatgt ctgcaatgcg
    28381 taaggctatt tctaaaggta tgactaactc ttacctcact gctccaagct ttacacttaa
    28441 ctatgatatt gatatgactg aaatgatggc tcttcgcaag aaattaattg atccaatcat
    28501 ggctaaaaca ggcttgaaag tcagctttac tgatttgatt ggaatggccg ttgttaagac
    28561 attgatgaaa ccagagcatc gatacctaaa tgcttcatta attaacgatg cacaagaaat
    28621 tgagcttcat aattttgtta atattggaat cgcagttggt cttgatgatg gtttgattgt
    28681 tcctgttgtt cacaatgctg atcaaatgtc actttctgac tttgttatag catctaaaga
    28741 tgttattaag aaaactcaag aaggtaaatt aaaatcagct gaaatgtcag gttcaacatt
    28801 ctcaattact aacttaggta tgtttggtac taagacgttt aacccaatta tcaatcaacc
    28861 gaactcagca atccttggag taggtgctac tattccgact ccaacagtgg ttgatggtga
    28921 aattgtagct cgaccaatca tggcaatgtg tttaacaatt gaccaccgta ttgttgatgg
    28981 aatgaatggt gctaagttta tggttgatct taagaactta atggaaaacc catttggttt
    29041 acttatttga ttccgtaggt cttaatttga tataataagt tattcgaaaa acgaaagagg
    29101 aaataaaaaa tggcttttga tgtaattatg ccaaaacttg gggttgacat gcaagaaggc
    29161 gaaatccttg agtggaaaaa aaatgaaggt gacaccgtta atgaaggtga tgtccttctt
    29221 gaaatcatgt ctgataaaac caatatggaa attgaagcag aagacactgg tgtactttta
    29281 aaaattgtac atcaggcagg agatgttgtt cctgtcactg aagtgattgc ctatatcgga
    29341 gaagaaggtg aagaagtagg tacgtcatca ccttcggctg atgcaactat cacagctgaa
    29401 gatggtcaat cagtttctgg acctgctgct ccttcacaag agacagttgc agcagcaact
    29461 cctaaagaag aacttgcagc tgatgagtac gatatcgtag tcgtaggtgg tggccctgca
    29521 ggttattacg cagcaatccg cggtgctcaa cttggaggaa aaattgccat tgttgagaaa
    29581 actgaatttg gcggtacgtg tttgaatgtt ggttgtatcc ctacaaaaac atatcttaaa
    29641 aatgctgaaa tcctagatgg tttgaaagta gcagctggac gtggtatcaa ccttgcttca
    29701 actaactatg caattgatat ggataagact gttgccttta aaaattctgt agttaagaca
    29761 ttgactggtg gtgtacgtgg tctcttgaaa gcgaacaaag ttgaaatttt caatggactt
    29821 ggacaagtaa accctgataa atctgttgtt attggcgata aagttatcaa aggtcgtaac
    29881 gtcgtccttg cgactggttc taaggtatct cgtattaata ttccaggtat tgaatcacca
    29941 ttagtattaa catcagatga tattcttgat ttacgtgaaa ttcctaagtc acttgctgtt
    30001 atgggtggtg gtgtcgtagg tattgagctt ggtcttgtat gggcttcata tggtgttgac
    30061 gtaactgtta ttgaaatggc tgatcgcatc attccagcaa tggataaaga agtatcgcta
    30121 gaacttcaaa aaatccttgc taagaaggga atgaagatta aaacatcagt tggtgtttct
    30181 gaaattgttg aagccaataa tcaattaacg cttaaattaa acaacggcga agaagttgtt
    30241 gctgataaag ctcttctttc aattggacgt gttccacaaa tgaatggtct tgaaaatctt
    30301 gagcctgaac ttgagatgga acgtggacgt attaaagtta atgcttatca agaaacttca
    30361 attccaggta tctatgcacc aggtgatgtt aatggaactc gtatgttagc tcatgctgca
    30421 taccgtatgg gtgaagtagc tgctgaaaac gcactcggcg gtaataagcg taaagcacat
    30481 ctagatttca ctccagcagc tgtttatact catcctgaag tagcaatggt ggggatgaca
    30541 gaagagcaag cacgtgaaca atatggtgac atattagttg gtaaaaatag ctttacaggc
    30601 aatggtcgtg ctatcgcttc aaacgaagca catggttttg taaaagtaat tgctgaacct
    30661 aagtataaag agattcttgg tgttcacatt attggtccag ctgcagcgga attgattaat
    30721 gaagcttcta cgattatgga aaatgagttg actgtctatg atgtagcaca atctatccat
    30781 ggtcacccaa cattctctga agttatgtac gaagctttct tagatgttct cggtgaagct
    30841 attcacaacc ctccaaaacg caaataattt tgtttaatat atttgagatt gagcttttat
    30901 agcttcaatc tcttttcacg tatgtattgt ctgaatatta taaaatatta gttaggaatt
    30961 taaaatgaaa tatatcgtta acacgtctaa tgatccggct tacaatgttg ctttagaagc
    31021 ttacgccttt caaaagttga cagatatcga tgaaatcttt attctttgga ttaatgaacc
    31081 ggctatcatt attggacgcc atcaaaatac aattcaagaa attaacaaag agttcattga
    31141 taaaaatggc atccatgttg tccgtcgttt gtcaggaggc ggagcagttt atcatgactt
    31201 gaataattta aactacacta ttatttctaa taatacacaa gaaggtgctt ttgatttcca
    31261 aactttttca aaaccagtta ttgatactct tgctaaatta ggtgttaaag cagaatttac
    31321 tggtcgtaat gatttagaaa tcaatggaca aaaatttgca ggaaatgctc aagcttatta
    31381 caaaggtcgt atgatgcatc atggttgtct tttatttgat gttgatatgt cagtcttagg
    31441 gcaagcactc aaagtatcta aagacaaaat tgaatctaaa gggataaaat ctgtacgcgc
    31501 acgtgttaca aatattgttg accatctttc agataaaatt acagtacaag aatttagcga
    31561 tgccatttta gctcaagtga aggaagaata tccagagatg gatgaatatg ttttgagtga
    31621 tgccgagtta tcagaaatcc aagctatgcg cgataatcaa tttgctacat gggattggac
    31681 gtatggtaaa gcacctgaat acaccattga acgtggtgtc cgctatccag ctggaaaaat
    31741 tacgacttat gctaatgttg aaaattcaac gataaaatct gtcaaaatct ttggtgactt
    31801 ctttggagtg aaacctgtag atgatattga aaaaatgttg gaaggtgtcc gctacgatta
    31861 caaagatgtt ttagcagcgc ttaaaacagt tgatacaagt caatacttct cacgtatgac
    31921 acccgaagag attacaaaag ctattgtaga ctaaaatcaa aaaacctagt caaagtcttt
    31981 gcctttggct aggttttttg tatggttaag tggccttata gtacgacggg catgtcgtac
    32041 atctgaggtg caagtcctct gtgggcaccc gctaccggtg aacccaatag cgactcccaa
    32101 gcttgactat cgtgaggtag cggggagagg aagggatagc gaaatcgtgg ctctacgaac
    32161 agaaacgtga tatgaaggcg tatatagcgg atgagggggc tataaactct aaagtccaaa
    32221 taggtagtcg taacctatat acgtaaatca cgagagtaaa cgaggaaaga tgtccgactt
    32281 atcccgtgag gtcctgtcaa gggtaaaacc tagtaacaac gaacgacaag aagtcagccg
    32341 aacccatagt agtgatgaag tttctgtaat ggaaatggaa cgaaggggtg aacaactaat
    32401 cgaagtaatc ctacttcact tgt
//
LOCUS       DATRME010000014        31462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRME010000014 DATRME010000000
VERSION     DATRME010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..944)
                     /locus_tag="VBC23_000673"
     CDS             complement(<1..944)
                     /locus_tag="VBC23_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEO6842641.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEA"
     gene            complement(1097..1576)
                     /gene="perR"
                     /locus_tag="VBC23_000674"
     CDS             complement(1097..1576)
                     /gene="perR"
                     /locus_tag="VBC23_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6842642.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            complement(1658..2098)
                     /locus_tag="VBC23_000675"
     CDS             complement(1658..2098)
                     /locus_tag="VBC23_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6842643.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            complement(2128..4770)
                     /gene="polA"
                     /locus_tag="VBC23_000676"
     CDS             complement(2128..4770)
                     /gene="polA"
                     /locus_tag="VBC23_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6842644.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            complement(4883..5695)
                     /locus_tag="VBC23_000677"
     CDS             complement(4883..5695)
                     /locus_tag="VBC23_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6842645.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            complement(5710..6324)
                     /locus_tag="VBC23_000678"
     CDS             complement(5710..6324)
                     /locus_tag="VBC23_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6842646.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            complement(6434..6640)
                     /locus_tag="VBC23_000679"
     CDS             complement(6434..6640)
                     /locus_tag="VBC23_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6842647.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            complement(6681..8915)
                     /locus_tag="VBC23_000680"
     CDS             complement(6681..8915)
                     /locus_tag="VBC23_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6842648.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMEPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAVGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            complement(8928..9344)
                     /locus_tag="VBC23_000681"
     CDS             complement(8928..9344)
                     /locus_tag="VBC23_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6842649.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            9507..10511
                     /locus_tag="VBC23_000682"
     CDS             9507..10511
                     /locus_tag="VBC23_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6842650.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            complement(10583..10951)
                     /gene="rbfA"
                     /locus_tag="VBC23_000683"
     CDS             complement(10583..10951)
                     /gene="rbfA"
                     /locus_tag="VBC23_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6842651.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(11041..13824)
                     /gene="infB"
                     /locus_tag="VBC23_000684"
     CDS             complement(11041..13824)
                     /gene="infB"
                     /locus_tag="VBC23_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6842652.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            complement(13844..14146)
                     /locus_tag="VBC23_000685"
     CDS             complement(13844..14146)
                     /locus_tag="VBC23_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6842653.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            complement(14139..14435)
                     /locus_tag="VBC23_000686"
     CDS             complement(14139..14435)
                     /locus_tag="VBC23_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6842654.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            complement(14457..15617)
                     /gene="nusA"
                     /locus_tag="VBC23_000687"
     CDS             complement(14457..15617)
                     /gene="nusA"
                     /locus_tag="VBC23_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6842655.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            complement(15644..16120)
                     /gene="rimP"
                     /locus_tag="VBC23_000688"
     CDS             complement(15644..16120)
                     /gene="rimP"
                     /locus_tag="VBC23_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6842656.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            complement(16478..16565)
                     /locus_tag="VBC23_000689"
     tRNA            complement(16478..16565)
                     /locus_tag="VBC23_000689"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16529..16531),aa:Ser,seq:gga)
     gene            complement(16609..17244)
                     /gene="trmB"
                     /locus_tag="VBC23_000690"
     CDS             complement(16609..17244)
                     /gene="trmB"
                     /locus_tag="VBC23_000690"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6842657.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            complement(17234..18034)
                     /locus_tag="VBC23_000691"
     CDS             complement(17234..18034)
                     /locus_tag="VBC23_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6842658.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            complement(18092..19126)
                     /locus_tag="VBC23_000692"
     CDS             complement(18092..19126)
                     /locus_tag="VBC23_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842659.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQAILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            complement(19128..19853)
                     /locus_tag="VBC23_000693"
     CDS             complement(19128..19853)
                     /locus_tag="VBC23_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842660.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            19925..20111
                     /locus_tag="VBC23_000694"
                     /pseudo
     CDS             19925..20111
                     /locus_tag="VBC23_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20163..20666)
                     /locus_tag="VBC23_000695"
     CDS             complement(20163..20666)
                     /locus_tag="VBC23_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842661.1"
                     /translation="MKLNKIILSTAALTALFLGYNSVTADTYNNYQPHRSNNMDLTEE
                     YNYNNQIELQERIKNLNIPFKEKLALLRKVKSRQDSYVLDILRKEVENKNSEYSELEQ
                     EYQRISALINAKFPQGKSSLAEKLHKELIWDLDGVKYDEGISKRTTLKKLEGKLNEYT
                     KKYSYLF"
     gene            21002..21421
                     /locus_tag="VBC23_000696"
     CDS             21002..21421
                     /locus_tag="VBC23_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6842662.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            21418..21714
                     /locus_tag="VBC23_000697"
     CDS             21418..21714
                     /locus_tag="VBC23_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842663.1"
                     /translation="MKIGRLIALGLVSLGAFELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(21778..23085)
                     /locus_tag="VBC23_000698"
     CDS             complement(21778..23085)
                     /locus_tag="VBC23_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6842664.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(23094..23654)
                     /locus_tag="VBC23_000699"
     CDS             complement(23094..23654)
                     /locus_tag="VBC23_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6842665.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(23608..24117)
                     /gene="tsaE"
                     /locus_tag="VBC23_000700"
     CDS             complement(23608..24117)
                     /gene="tsaE"
                     /locus_tag="VBC23_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6842666.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(24200..25621)
                     /locus_tag="VBC23_000701"
     CDS             complement(24200..25621)
                     /locus_tag="VBC23_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6842667.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(25724..26335)
                     /locus_tag="VBC23_000702"
     CDS             complement(25724..26335)
                     /locus_tag="VBC23_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6842668.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            complement(26425..27009)
                     /locus_tag="VBC23_000703"
     CDS             complement(26425..27009)
                     /locus_tag="VBC23_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842669.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            27098..27910
                     /locus_tag="VBC23_000704"
     CDS             27098..27910
                     /locus_tag="VBC23_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6842670.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            28212..29222
                     /gene="manX"
                     /locus_tag="VBC23_000705"
     CDS             28212..29222
                     /gene="manX"
                     /locus_tag="VBC23_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6842671.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            29255..30067
                     /locus_tag="VBC23_000706"
     CDS             29255..30067
                     /locus_tag="VBC23_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6842672.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            30082..30993
                     /locus_tag="VBC23_000707"
     CDS             30082..30993
                     /locus_tag="VBC23_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6842673.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            31112..>31462
                     /locus_tag="VBC23_000708"
     CDS             31112..>31462
                     /locus_tag="VBC23_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6842674.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFK"
BASE COUNT         9900 a         6235 c         4888 g        10439 t
ORIGIN      
        1 aacgcttcat atggaatcgt ttcgttaaca cctgctaaaa atggaccttt agcttttgca
       61 gcactaacat attttgctgg gacgtctact ttttctttaa aggccatctt tagagaattt
      121 tttacttcca tatcagttgt aatgtgagga atgttaatgg ttaccatttt ttggctaatc
      181 atgtttttta aatcaacttt accatcttta gcaccataaa ctctaattgt ggcttggtag
      241 ctatgtttat cattagcttt aatttgatct aagagagctt gcccctcttt acctgtcgta
      301 ttaccattta aagaaacttg atagaaatat ttacctttag ttaagccttt caatggtaat
      361 aatggactat ttttatcaac accattgtct gaccattttg ctccctctga agctttaagg
      421 attaaacgcg ttaacatgcc gtctccacca aacgcttcat atggaatcgt ttgatttaca
      481 cctgctagaa atgggccatt cccttttgct gcattaacat aatgagccgg aacgtctact
      541 gtctgtttaa aagcagtatc agttgatttc tttacctcat ctacactagt cgttttggtc
      601 atcgctacag gtgtcggcgt tgccatcatt tttctaacag aggtagccac catcataggt
      661 ttgtcactcg ttttttcaac ttttgccata ttttgcattt taggcatttc tgaacttgtc
      721 tccatcgtag taacttttga taatgcctta tcagtactac tttctacatt tgtagaagtg
      781 ttagctgttg gggctgtaat ggttgtttta ggttgggaca tctcatctgc tgaaacaata
      841 tgagattgcc ctaaagcaac aatgcttcca ctagcaatca ttacagatgc agctccaact
      901 ttcaatcggc gaattgacca tttattgtat tgtgatattt tcataaggga ttcctctttc
      961 tttttgttaa tgagagtata ccaacaaaaa ataaatcatt ttttaagcaa ttataaatta
     1021 tttcttaatt attacttagt aaaacttaaa ctttttctct tagtctagaa acaaaaaaag
     1081 attgaacaat tacgtttcaa tcttttttat atttatcctt acgcttcctc tgacactccg
     1141 gacagatacc ataagctact aactgaactc tagtcacttc aaaacctgtt tgttggtgtg
     1201 cttctctaga aatatccaat aagtcaacat cgacaaaatc aacgattttc ccacaatctt
     1261 cacaggcaat gtttaggtgc tgatgcccca taaaatcata ataagttgta ctataattgc
     1321 aaagttttag ctctgtcaca aacccctcat ccacaaggac cttaaggtta ttgtaaactg
     1381 ttgccaagct catgttagga tattctggta ataaatcatt gtaaatcttt tctgcactcg
     1441 gatgctcacg actattaacc atataggaaa taatagcttt tcgagtctca gtaattcgaa
     1501 tacgtttttc acggagatgc tctaggacgt tttcataagc atcgagaggt ctatgaccat
     1561 gtgaatgact gtccatagga ctcctttcca gcacgaactt taatttagca tggttattat
     1621 agcataattt atattatttg cctattaaac acggtaatta gttagtttct gagtgtaatt
     1681 tttggtattc aactttcaga catttattca tgacaatctg tttgtgtcct gcttgcttta
     1741 gaattgtctc tgcttcttga ctttcaagtc caagctgagc ccagaaaata tcagcgtcta
     1801 cttctaaaaa ttctcttgca atatcaggta aatactcgct acgtcgaaaa atattaacaa
     1861 tatcaatatg ttcagtgaca tccttaagtg aggcatatgt catttgccca agtattttct
     1921 gtcctgcatt tttaggatta actggaataa tttgataacc tgcttcttgc attatttttg
     1981 atacttgata agctgcagtt tcttgacggt ctgacaatcc tacaactgcg atggtctttg
     2041 cttttgttaa ataatttttt aacataaaat cacttagatt ttgaaaatga taaaccatat
     2101 taatgactcc tttacgtatg ttgtccacta cttcgcttca taccaagtct cacctgcgtt
     2161 ctcatctgca atcaaaggta ccgataaact aattgctgac tccattgttt ttgtaaccaa
     2221 ctccttgatt gctcctatct cctcgttagg gacttcaagt acaatttcat catgaacttg
     2281 caacagcatt tttgatttat aaccaccttt atcaagtacc ctatctaaat ttatcattgc
     2341 aattttaaga atatcagctg cacttccttg gattggtgaa ttgattgctg tgcgctctgc
     2401 aaattggcga atgttaaaat tacgagaatt aatatctggt agtgaacgac gacgatggaa
     2461 aagtgtttca acgtaaccct tatcttttgc ttcacgaaca actgtttcca tatagttttt
     2521 tattccagga taacgctcaa aataggtgtc aatatattgc ttcgctagtt tacgaggtat
     2581 tcctaaatta tgcgatagac caaaatctga aataccataa acaattccaa agttaacagc
     2641 cttagcattt cgacgatcat tgggagtgac attctcaggt ttttcgatac caaaaacacg
     2701 catagctgtt gaagtatgaa tatctgctcc ctctttaaag gcagcgatca aatgttcgtc
     2761 ttttgaaata tgtgctaaca cgcgcaattc tatttgagaa taatctgaac tcaataaaac
     2821 agcattgtct tctgatggaa caaaagcctt tcggataagc cgtccttgct ctagtctaac
     2881 tggtatattt tgaaggtttg ggtcagaact tgaaagacga cctgtctgag tcaaatcttg
     2941 aacataacgt gtatgaattt taccatcttc taaaatataa tcttgtaaac caataatata
     3001 ggttgattgc aatttagtga tttgacggta ctctaaaatt ttagttacaa gaggagaaat
     3061 cggagctaag cgctctaata catctactgc agttgaataa cctgtttttg tttttttagt
     3121 catttctaca ggaagaccta acgtttcaaa caacaacttt cctaactgtt ttggagaatt
     3181 aatattaaac tcttgcccag ccaattcata gatctcttgt gttaaggttt cgatgacttt
     3241 ttgattttca atagccattt cattcaaggt atttttctta accttaatac ctcgaatttc
     3301 cattttagct aaaacattcg ctaaaggttg ctccatttca aacagcaatt cttcttgctc
     3361 attagcttta agctgttgca taatattagc ctcagaatcc actaaaactt ttactttttt
     3421 agctagatgc tcaagaaatc gtgttttttc tggtatagcg cgtttagcac ctttcccata
     3481 aacagcatca tcagtatcca gtggcagatt tgtatataac ctagcaattg ttgatactag
     3541 attatcctca gtagtagaca aaaggtactt ggccaatcta gtatcaaagg ctggctctgg
     3601 taaatcaata ttaaaacgat ttaaaaggac cttacttctt ttaaaatcat aagttttgat
     3661 aggttttgat aaagcttttt taaataaagc ttcttgcaat aacgatacat cactagtagc
     3721 gtagattgta tcgctatttc cccaagctat cccaattaaa tcttctacgt ggtagttatc
     3781 acctaacatc tcaaagtaat aaaaatcatt atcggagaac atttctggtt ttaattgctc
     3841 cacttcagta aagtcaacct caaaatcttc ctgacttgtc tcctcaccca gagctgcttt
     3901 aaactgttta aaatccatct catcataaaa ttgagacaag gcttttatat cttgcggtcc
     3961 actataaagg atatcttcta agccgattgt tataggtgat gctatgttga ttgtagctag
     4021 ggtctttgat aagaaagctt gctccttatc attgataagg ttttctttca tcttagactg
     4081 tttcatagcc tcaatatttt catagatccc ttctaaggaa ccatattctg atagcaactt
     4141 caagccagtt ttttcaccga ttttagtaac cccaggaata ttatctgatt tatctcccat
     4201 caaagctttt aaatcaataa attgactcgg cgtaattccc attttttcca taaggtaagc
     4261 tggcgtgaat tcttcaaact cagcaactcc ctttttagag atttctacta ccgtgttagt
     4321 gtctgttaat tgaattaaat ctttatcccc agaaaccacc gttatatcaa aatgctcttt
     4381 actagcttcg gcttgttttg caagagtacc aatgatatca tcggcttcat aatgttccaa
     4441 ttcataatgt ttgatgccta agacatctaa ttgttgacga atataaggaa attgttcacg
     4501 aaattcatcc ggagttttag cacgcccacc tttataatct gcatacattt ccgtacggaa
     4561 agtagttttt ccagcatcaa aagccactaa aatatgacta ggctggacac gacctaaaat
     4621 atgattcaac atcaaatgga aaccatatat ggcattagta tgaagtccag aattgttttt
     4681 aaagcggtca atttgattat aaagagcaaa aaaagcacga aaggctacag atgagccatc
     4741 aattaaaagt aatttattct tgtttgtcat aggtctatta tagcataagc tatctgtaaa
     4801 cttctgaatt ttgttagttt attaaacaaa agaaccctaa acaaacagtg tttaaagttc
     4861 cttttattat tctatatcta ttttaacgta aagcatagtc ataaatagct ttagcaaccc
     4921 cggcttggtt attatccacc gttatatatt tacatctaga tttaatctca tctgatgcat
     4981 tccccatagc aacgctattg ggaactaaat ctaataattc aatatcgtta ggagcatcac
     5041 caatagccat aacttgatta atatccagtc ctaattttgc tatcaaggat tttaagcctc
     5101 ttgcttttgt tactccttga ggcataatct cataaatata gtcttggctt cgaactccac
     5161 taaatttcga gattaaggct tcttcaacag cttcttgaaa agcagttaca tctgcatcta
     5221 atcccatgta cattgcttga aagacaattt cttcttgatt gcgaagagca tctaaactta
     5281 ttgactttgc ttttgtaaaa actaaatcag catcataagc aacaatttca ggaacttcct
     5341 caccaacagc gtaaaatgta ttttcagctg ttaacgttaa acaaacgttg ggaaattctt
     5401 taacaacctc ctcaagaaag atgagttcat catgtgttaa agttgcagaa tcaatcaatt
     5461 gccaatcttt cgtgctataa gtgctacaac cattattcat gataatatac tcctcttttg
     5521 ttaaacctag ttcattaaaa taaggaagta tacctgacat cggtcgccca gtacacagta
     5581 caattttaat acctgcttga gttgcttctt taatagcttg gatattttcc tttggaattt
     5641 tcttttcatc atttaaaagc gttccatcca tatcaatagc aattaatttt atcatctctt
     5701 tctcctctat catttcttac gtggtctgac aactggcaag tgccattgtt tcttataccc
     5761 caacatcctt aggctagtta caactaacac taacaaataa tagtcagttg ttgttttagc
     5821 aattttaaaa tataaaatga tccccactaa tattgaccaa cctgcatata tttcacttct
     5881 tagtacccca ggttttcggc cagccagaac atctctgaca ataccaccac ctgcaccagt
     5941 tagtactgct gcgacaatga cagcactaag aggttgatga gattgagtcg catatagtgc
     6001 tccttgaaca ctaaacgctg ctaacccaat agcatctgat acaacttcgg catcccgtcc
     6061 cttaccactc aacaagttag ggaaaatcat aataaacaaa atagctgctg ctgctgcata
     6121 aaaagcaatt ccttgactcc aaagtgtttc aataggcaag ccaattagta catttctaat
     6181 agctccacca ccaaatgccg ttacaaaacc taagataaaa agccctagaa catcaaattc
     6241 ttcctcaatc gccacaatag ctcccgaact tgcaaaagct acggttccaa ccaaacttaa
     6301 aatgttccaa atatcgattg acatctgcac tcctcacaac taattctatt ctagtttatc
     6361 atttttagaa taaaaaagaa gtgagaaact gctcaaatta agaaattaaa agctgtaacc
     6421 acttctttag ttattaattt atttcctctt ctagtgaata ctttgtgtct tttaaagaac
     6481 gttttaaaag acatttgaat gtttttccac ttacgacaac ttgattttta gtcaaatcaa
     6541 cattaacctc gtcaacaccc atcacacttg ataacttatc agaaactgtt ttggcacaac
     6601 catcacattt catgccacta actcgatagg tatgtttcat attcttctcc cttcataaaa
     6661 attcagtctt attatcttta ttattttaag acttttaagc gaagtgcgtt taaaactact
     6721 gaaactgaac taaacgccat agctaagcct gccaacatag gattgagtaa gggaccacca
     6781 aaaagatgta aaacacccat tgccactgga atcgctaaaa cgttataaaa gaaagcccaa
     6841 aataaatttt ccttaataat ttgaatcgtt tgtttactga tagtcattgc ttttaagaga
     6901 tctgttaact caggtttcat caacacgata tctgctgatt caatagcaat atctgttcca
     6961 gaactcatag agatacctac atcactgcta gctagcgcag gagcatcatt gatgccatca
     7021 ccaaccatcg caatctgtcc gcctttttct ttaagttcta aaataacgtt tgctttctga
     7081 tcaggtaaaa cttctgaaac aactgtcgtg atacccaatt tttgtgctat ggcatctgca
     7141 ctagccttat tatcaccagt aagtaaaact aaattaaatc ctaatctttg taactgctta
     7201 actgtttctt ggctatctgt ctttagctgg tccgttaaag ttattaaacc gctcaacact
     7261 ccattttcgg aaacgtaaac cacagtttgt gcttcttctt gagctatttc tatatcagcc
     7321 tttgctaaat ctaaattaac atcgttctta gtcattaagt tagcattacc tacaagcata
     7381 gttttaccat taataattgc actcaaaccg taacccaaaa tatttttaaa agccatgact
     7441 tcccttaagg gaagttcttt ctcttttgct ttgttaacaa ttgtttggct aagtggatga
     7501 tttgaaagtt tttctactga tgctacttga cctaaaaact cctctttatc taccccatat
     7561 gttttgatag caactacttc tggtttccct ttggtcaagg ttcctgtttt atcaaaaact
     7621 attgtatcaa tgtggtgagc cttttccaac acttcaccac ctttaaataa aataccattc
     7681 tcagctgctt taccactagc caccataata gctgtaggag ttgctaaccc taaagcacat
     7741 ggacaagcaa tcactaagac tgcaatcata atcttcagag agaaggcaaa actttgcccc
     7801 agaattaagt accaactcaa acctgataaa attgcaatga caataacaaa agggacaaag
     7861 actcctgata ctttgtctgc taagttagca attggagcct tacttgcctg tgcaacttct
     7921 actaagttaa taatttgtga tagaagggaa ccgacagctt ctttttcaac gaaaatcgtt
     7981 aagctacctt gcccattaat gctggcaccg taaaccttac taccaacctt tttttcaact
     8041 ggaatacttt cacctgttaa catggattca tcaatacttg attctcctgc tacaacatgg
     8101 ccatcaagag gaattttttc tccaggtttg acaagaattg tttcacctat tttaacttta
     8161 tccaaaggaa ccatatagtc ttcaccatct ttgataactc ttgcttcttt tgctgataaa
     8221 gttaataatt tttcaattga ggccgaagta cgccctttgg ataatatttc aaaatatttt
     8281 cctagagtaa taagtgttaa aataactgca acagattcga aataaagctg gtgagcgtag
     8341 tgtatgtcac cctgtaatat ttcgtacaat ccatataagc tataaatgaa agccgctgtt
     8401 gtggctatag caactaatga atccatattg ggatgccttt taaaaagtgc tttaaaacca
     8461 ttttggtaaa agatacgccc aaaatacatc accggtagtg ttaacaataa ttgtatcaca
     8521 ccatagttta gaggtccctt atctggcatc aaaaatgatg gtaaccataa gttaatcatt
     8581 ggctccatgg aaatatagag aagaggtata gttgcaactg ctgacatcca aaagcgatgc
     8641 cacatctttt caattgctaa ttggtgccgt tcagattggc ttttagaagt ttcctctttg
     8701 aaaagcgatg cttcgtatcc agtctcagaa acacactctt caatcacgtt tggcgataag
     8761 gttgtatcat caaacgtaac tgtcattttc tctgttgcta aatttactac tgcactatcc
     8821 acatggtcta acttgttgac agctttttct atcgttaaag cacaagatgc acaagtcatc
     8881 ccatcaatca aaaatgtttc ttttctatta gccatatttt ctcctttcta atggcattga
     8941 cactgaccag gaatgcaatt acaagctaca cgttcaagtg ttttgccttt cttagcttct
     9001 agtgatgctt gcaaatttaa tcgatccttc tctgtcattg gtatactttc caataagtgt
     9061 ctaacaatag caacatgttt ggtctggcaa actttctgca atacactatc tacctctgac
     9121 atcatagcta aatcttcatc aattaaagaa gaatagctaa atccttttcc cattttttct
     9181 ctgctcacgt agcctttatc taagagcctt cttaataagg ttttgacagt tgaaggagtc
     9241 caatcatact tctctagtaa aactgctagt atctcattac tggttgtatt ctgctgtgcc
     9301 caaaccactc tcataatttc ccattctgcg tttgaaatag tcattttctc acctctttcg
     9361 atttacgttt gtaaaccaat tttagcaaat aaatctacat ttgtaaaccg aaaaacattt
     9421 ctcatctttc tttattctat tgacaaagat gttggtttta ttataataat attacacaaa
     9481 aataacatta tattaaggag aataccttga aaccgtcctt caaaaaacta ttgctactat
     9541 ttagtattat caccatttta tccatcgcct gtacaccaca tgcaaaagca agtgggcgtt
     9601 catggaaaag ttggtttatc gaacaatact tttggctaaa gcgcgataaa agttattata
     9661 aagttcaaga tgaaagctca tttcaaaagt acttaaatgc tagccgtgaa caatctgata
     9721 agggttatta cttagaccca aactctgtta atggagggct tgttcaagag agactttttg
     9781 atatgcaagt ctattcttgg aatgacaacg gtaaagctaa tcaaaagacc attttctatt
     9841 tagctggtgg ctcttactta aataatccaa cgccttacca tatcagcatg cttaagacgc
     9901 tctcaactag tcttgatgca aaaattattt taccaattta tccaaaaact cctcgttata
     9961 cctatgatta tgctatccct agattggtta acctttatag acactttcat gaaaaaaacg
    10021 ctaatctcac tttgatgggt gattcagctg gcggaggatt agcactagga ttagcgcatg
    10081 cattgtctca ccaatcaggg caagaggcta ttcctcaacc taaaaatatt atcctcttat
    10141 ctccatggct agatgtgaca atgaaacatc cagaaattcc caaatacgaa gataccgatc
    10201 ctatcctatc tgcttggggt ctcgcacgtg taggtgaaat ttgggcaaat ggttctaata
    10261 atactaacca tacatatgtt agtcctaaaa atgcccctgc tactaaactc gctcctatta
    10321 cgttatttac tggaacacga gaaatattct tcccagatat tcgcgattat gctgcacaac
    10381 tccaagcagc taatcacccc gttaactata ttgcacaaga gggaatgaat catgtttatc
    10441 caatttaccc tattgaagaa gctaaaacag cacagtatca aatgattgat atcatcaata
    10501 aaactcctta atactagcta tctcaactct aaagctataa aaaataaacc atgattttga
    10561 catggtttat tttttattat tcttaatctt ttttatctag atttcgcaac atttcatcaa
    10621 tcttatttcc gtattcaata gattcatctt taacaaattg taaatctggt attttataca
    10681 tagtaaggtt tttgccaagt tcacgcttaa tagtaccagt tgctttttct aaaccaattt
    10741 gtgctttttg attatcagaa gcaagtgtcg aatgaatagt ataaaatact ttagccattg
    10801 aaaggtcacc caacatctga acatcagtaa ttgtgacatc ttgtacacgt ggatcattaa
    10861 ctcggagacg caaaatctca ttgacttcac gtttaatttc cattccaacg cgatcaatac
    10921 gatgattagc cataattaat cctttcttca ttgtgctcat aacttgaaag ctaggacgag
    10981 cctagccata attttgtatt ccctaccaac taaagataca agttgggttt ctcatagggg
    11041 ttatttacgt ttaatttctt ccataatgta tgcttcaata gtgtcatctt ctttgagatc
    11101 attataattc tcaatcatta gaccaccttc ttgagcattg ccgacttctt taacatcatc
    11161 tttatagtgt ttaagacttg ctaatttacc atcaaaaatg acaacaccat cacggataac
    11221 acgaacactt gaatcacgag taactttacc attgataacc ataaatccac caatagttcc
    11281 cacttttgaa actttaaagg tctcacgaat aatggcttca ccaaggattt tttcttggta
    11341 ttcaggatca agtttacctt tcatcgcttc ttcaacttct tcgataactt tatagataat
    11401 gctatgttga cgaatttcga catcatcagc atcggcttgt tgacgagctt gtggagtagg
    11461 gcgtacgtta aatcctataa taacagcatt tgatgcttca gcaagagtta catcagactc
    11521 attaatagca ccaactgctg agtgaacaac attaactttt actccttcaa catcaatctt
    11581 aaggagagaa gctgcaagtg cttctacaga accttgcaca tcagctttga taataacatt
    11641 aactgactta acttcaccag ctttaagggt atcaaataag ttctcgaggc taacacgttg
    11701 cgtgttttgg cgttgtttta agagagcacg ttttgcacgt tcttcacctg cagcacgcgc
    11761 agctttctca tctgcataaa cagcaaaatg atcaccagcc attggagctt cgttcaaacc
    11821 agtaatagaa actggtgtag atggacccgc aactttgaca cgacgcccaa gatcattagt
    11881 catcgcacga acacgtccaa atgtattacc aacaacgatt ggatcttgta catttaaagt
    11941 accttgttgg acaagaagcg ttgcaacggc tcccttacct ttatcaagac gggcttcaat
    12001 aacagtacca atagcacgaa catctgcatc tgcttttaat tcttccattt cagcaactaa
    12061 aagaactgtc tcaagtaatt cttgaatatt tttaccaaac ttagctgaaa tttcaacaaa
    12121 ttcagactca ccaccccatg cagtagagat gacaccatgc tcagctaatt cactgataac
    12181 gcgttctgga tttgcaccag gtttatcaat tttgttgata gcaacaataa ttgggacacc
    12241 cgcagcttta gagtgattaa ttgcctcaac tgtctgcggc atgacaccat catcagcggc
    12301 aacgataagg atagtaatat cagttactga tgcaccacgc gcacgcatag aagtaaaggc
    12361 cgcatgccct ggtgtatcca agaaagtaat tttcttgcca gcttcttcaa tctgataagc
    12421 accaatatgt tgagtaattc caccagcttc gccagtagcg acacgtgaat tacgtagagt
    12481 atcaagaaga gttgttttac catggtcaac gtgtcccatg atagtaacaa caggcggacg
    12541 ttccgttaag ttatcaggat taaggtaatc ctcatctgca aagaagcgtt caatatcagc
    12601 ttcatcaact tcaaccttag catgtgcttc aatcccgtag tcaaccatca atagctcaat
    12661 agtgtctcca tctaatgatt gattctgcgt tgccataact cccatcataa agagtttttt
    12721 aacaatttct gctggttcgc gtttaatacg ttttgcaatt tccgcaacgg tcatgccctc
    12781 agtatattca aattcttttg gtaattcatg gaatttacgc tctgttactg gttttggagc
    12841 tccattagtc ttaccttttt tatttttctt attatgattc cagttactat ttctttgatt
    12901 tctcacttgg ttttgattat tccagttttt cttatttttt gtttgctttt gaccatcttc
    12961 attttcattt gaaaattgac gagacttatc agaacgagtt tgttttttac gacgactatc
    13021 tgctgtctgt gatggcgtag cggctacttt attgacaatt tcttttacct tagcaacagg
    13081 tttagaagca agtttttcct tagctttttg agcttcttct tgcgctttta acgctgctgc
    13141 tttagctagt tcttgctcct tagctgcttg acgtttggct tcttgctctt cacggaaacg
    13201 ctgctcacta tgacgtgagt attcagcatt ttgctcagct ttcaaagctg ctgctcgagc
    13261 tttaaagtca atgcgattgc ctgcaggttg ctgaggacgc gtttggggtt tctgtttata
    13321 atcagcataa cgcccatcat ttttattacg gttattttgc tgaccttgtc ggttatcatt
    13381 acgattacca tttgaacgct tttgaccttg atggcgtctg tcgtcagagc gtgtatcatt
    13441 atggcgatta ttcttacggt gatccccatt atgttgacgc ttctccgctt cagctttagc
    13501 acgagcctcg cgctccgcct taaagttacg actttgtggt ttcggtttag ctacaggttg
    13561 agttgaaggt ttatcatcgg acaagccttg tttggtttca acagttttag attctgtttt
    13621 cacaccttga ttagtttgaa cagaatttgt tggagcttta gttttagaag agctaaaact
    13681 ctcaacaata cgtgtagcat cattttcttc aacgctagaa gcatgacttt ttacaggtaa
    13741 gccaagcgat tgagcttgct caaccacttc cttactcgtt tttccaattt ccttagcgat
    13801 ttcgtgtaat cttttctttg acaattgatg tcctcctatt ctgttatgcc ataagagtcc
    13861 tcattttctt tgaaaatcca gcgtctgcga cagcaaccac ttttcttggt ttgcctaacg
    13921 catcacttaa ttccagtgtt gaaaacactg tggagacttc tgttttgtag taattacttt
    13981 tatcagttac ttttttcgtc aaatttgggc cagcatcatt tgctagaaag attagcgata
    14041 cttgttgatt ttgaattgct ttaataacca attcttcacc tgtaataagg cgccccgcac
    14101 gctgtgctaa cccaatgaga tttaatactt tttctgaatt attcaaggcc taactctctt
    14161 cttttgacct tatggtcgac atatgccaat agttcatcat agaattcatc agaaacttcc
    14221 attgaaaaac tgcgatcaaa cactcgtttc tttttcgcta ggatagcttc atcattgtcc
    14281 aattttatgt aggcacctcg accattttgc tttccggtag gatctataaa aacctgtcct
    14341 tctttatttt taacaattcg taaaagatct cgcttatcga tcacttcacc agaaacaact
    14401 gattttctta aaggtatttt ttttgtttta gccataaaac acctctctca tttatattat
    14461 tctgccttat ctgaaataac ttcagattct tgagctactt cctcagtttg aagttcttgc
    14521 gcttccattt tttcgtattc acttgcagat ttaatatcaa tacgatagcc agtcaaatga
    14581 gctgctaaac gcacattttg tcctcggcga ccaatagcta gcgataactt gctatctgga
    14641 acaactacag tagcacgttt tgtatcttca tcatcaaaaa gtaccatatc aacttcggct
    14701 ggtgcaatag cattgtaaat gaattccgct ggatcttcaa cccattgaat aacatcaatg
    14761 ttttcttcaa ctgggatctc caatcctgtt ttagcatcaa cgcgtttagg atggaaatta
    14821 ctgataactt ttttaatgtt acttccaccg cgtccaacaa ttgttccaat cgcatcaacg
    14881 tttgaattat gactgcgtac agcaacttta gtacgatctc cagcctcacg agagacgctc
    14941 ataatctcaa cagttccatc aaatacttct ggaatttctc gctccataat gcgtttgata
    15001 aactctggat gactacggct aacaaaaacg ttgacacctt taggattatt ctccacttta
    15061 tagacataaa cgtcaatcat atcgtgagat ttaaagctct ccccaggaat ctgatcttga
    15121 tgcgataatt gcgcctcgag tgaaccgaga ttgacataga taaaacgttg gtcaaatcgt
    15181 tcaacagtac cagtcataat ttcaccttcg tgttgtttgt attcattgaa ggttacttca
    15241 cgcatttgac gacgcatttt ttccataata gtttgttttg ctgactgagc tgccacgcgt
    15301 ccaaattctg taacagattc ctcaaagcga atcttatcac caagttcata cgctgagcta
    15361 atagcaagtg cgtcttttag gctaatttcc aaacggctat caaaaacttc atcaacaact
    15421 tcacgaacag tatagacggt aaagtctgct ttcttctcgt taaattcaat aacgcatgat
    15481 tctgattgac cataacgacg cttataggca gattttagtg actcagtaac tgcatcaata
    15541 atatcttctt tattaatgtg tttttcttct tccaaaatac ggaaggcttc tagcatttct
    15601 ttgctcatag tgtccattct tgcttcgcaa gaatcatcct ttcttaaagt ttaaccgcaa
    15661 gacgggcttt tgcaaccgtc tgatagggaa tatctacagt tttatgacgt gttttgtcca
    15721 tataatcaat tgtcaaggtt tcaccatcaa atgataataa gtcaccttca aatattttaa
    15781 ctttatcaat ggatttatac agacttacat tgatataaga cccaacagca tttgataatg
    15841 cttctgctgt tttaagtggc ctttccaacc caggacttga gacttcaagc atatactgtt
    15901 ctggaaatgg atctggttga attgtgtcaa gtaagggact tacaacgtct gtcaattgtg
    15961 ctgtatcttc aaccgtaatg cctccgggtt tatcaattaa aatactgagc acatagtcgc
    16021 cacccatttt ttcgtattct acatctacta attcaaacgg tgcttgaata gcaggagtta
    16081 cgacttgagt aacaatatca acaatggttt gatttgcgat aacagctacc tcttctttct
    16141 aacttgcttt agaacttaca atgactataa attctatgaa tcacacacat aagaggcgaa
    16201 gtaaaactcc gcccctttct gtattattta atactattat agcacaaaat acgattattg
    16261 caatcattta ctttaaatta gcatcaccgt acactaatct tatatcaaaa aattaaaatt
    16321 tgtcctcaat tctttgtaag agcttagaaa actttatgct aatacttcaa gctcaaagag
    16381 aaacgatgat aaaataaaag aaatttttcg aaacttggac ttttgacgag tgaaaaaggt
    16441 gaacacttaa taaacactga tacattaaca agattaaagg agaggagggg attcgaaccc
    16501 ccgagccccc attagggact acacgctttc caagcgtgcg cactcagcca ctatgcgacc
    16561 tctccataaa ggagtagcaa ataactaccc acttactatt tacttattct aaaatcgtgc
    16621 ttctacacga tagatgacct gtcctttatt tgaaaatttg cgttcgtact cagtcatgat
    16681 attttgctgg taatcacttg catgtaaatc taaccaaact tgttttaaag tcataccgta
    16741 ttgagaaaag ctagcaaggc tatattcaaa taaacctcta ttatctgttt taaaatgaat
    16801 ttccccctgc tcaggaagta tgtctttata ggtatccaag aaagtcttat aggtcaatcg
    16861 acgtttctca tgctttttct taggccatgg atcactaaag ttaagataca tcaaatcaac
    16921 ttcgccagta tcaaagtagt ttgatagact agaaccatca actaatagta atttgatatt
    16981 cttagcaccg ctatctagaa ctttatccag agcataggaa agcactgaca attgaatatc
    17041 aattccaata taattgatat ctggattttg ctctgccata ccagtgataa aagccccttt
    17101 tccggaacca acttctatgt gaatggggtt attatttcca aaaatctcat gccaacgccc
    17161 tttggcttct tctggatttg aaataacata gtgcggatta ttttctaagt gttcctctgc
    17221 acccttacgt tttctaactc gcattatact ttttaattaa ctctctaaac tttcgtaatt
    17281 cataaatttc ttgattgaca tgctccatat ctcgtttatc aaaacatttg attatctgtg
    17341 ataaatatga aaattgccca taccaaataa ttttgctcca gactttctca ttatctttat
    17401 aaccgtagta agacagccaa tctttccagt gtttttgtgg aatataatga cttaaaatat
    17461 atgcaacgtc atacatgcga tctgttaaac gaacagaatc ccaatcaacc aaataaatta
    17521 aaccacttgt tgtaataacc cagttgctat gtttgatatc tccgtgaacg attgttgcaa
    17581 cctctgttcg aaactcaggt aaactacgct ttaactcagt cacaatagat tgcaaataag
    17641 tgttttctcg gatttgaata ggagtttgtt tttcccaatc catcaacaaa tcataagggt
    17701 tttcaatttt ataccctaat tgtaacagtt gatttactaa gggacgagac ttgtgcaggc
    17761 gcagtaaaat atgaataatc tgtttactac ccatatcttc tttagtcaat gttcgaccat
    17821 ctaaccattc ttgagcactc atcatatcgc cattacttgt acggcgtgcc cacagtagtt
    17881 gtggcgcaat ctgttcctta gctaaggctg gcaaaatagg tgtcgtatta tatttgacaa
    17941 agacacgttc accatttgga taagtaccaa tataggcttt gccacttttt cctcttaagg
    18001 gagtaagcgt caattcttga tttgatattg tcaccagcag tccctccttt tcatttagtc
    18061 gtttcctctt attttacttg ttttataggc tttagtcaat catcttcttt attttgtaag
    18121 ggatgtaata ttccgtcaca attaacatca aaactatcaa taatataaca ggtttcaatt
    18181 ctcttataag tatgatatca attactgtca cagcatatga taacactctg agaaaacctt
    18241 ttagctgact tgctttagca tttaatctaa ctggatagag ctgattcatg tattgataat
    18301 caaaatggtg acctaaagct aataattgga acaataataa gtagttaaat acaaatgcca
    18361 gagcaagggc gaggtaggtt tcgttaacaa agatgactga taagatattt aaagttacca
    18421 accttatggt taaaccaaga taatctgagc ttcttagaaa tgccctcaca aataagttag
    18481 tccataaacg cgatggcgtt ttagaaataa gttttaaaat aacatctaaa aatgaacgac
    18541 gttttacact tgtcgaaatt cccttaacat ttgtaaacaa agagaaaaat tttaatatag
    18601 cctgttttct ttcctgctca tgatttatag ctgcatccca gttcaaattt tgattgttat
    18661 aaaagacctt taccttgtaa gttatcacca accatttgat tattcctaga ccaaaaatta
    18721 acaatgtaat cataaaaact gacaagccta actttatcaa aattggtgat attaatacta
    18781 aaaataaggt ttgaacaacc aaccaaaaga tataagtccg ttttttagca gaatttataa
    18841 tttcctttat tgcttcttct tttataagta aaaattgttt atcagccggt tctaaatagc
    18901 tggctatttc ccccatcgct agtaacatta agataatgat tgacacaatc acgataattg
    18961 gccaatgtgt ttttggaaag tcttttagta actggctata ctgatataac aaaaaacctg
    19021 acaaaaacat taatactaat acaaaatgat cattaaagac gtatctcaaa tatttactat
    19081 tttgagttaa aaagagactt cgacgtttat taaataactt tttcatatca cacctctttt
    19141 gtcagagcta tgtagatgtc atttaaatta gcattactgt ctccaaaaat agcccgtaat
    19201 tcctctaggg ttccaactgc ccgtatttct cctttatgta aaataacaaa acgatcacac
    19261 attttttcgg cagaatctaa tacgtgtgtt gacatcaaaa tagatttccc tttagctttt
    19321 tcttctgcta gaagattaat caaatctgaa atagctagtg gatccaaacc taaaaatggt
    19381 tcatcaacaa taaataaact tggagataca acaaaagcac agataatcat aaccttttgt
    19441 ttcattccct ttgaaaaatg cattggaaac cagtctaatt tatctgttag acgaaacatc
    19501 tctaatagtt tttgcgcacg tgccataact tcatcggtgg caatatcata cgccatagct
    19561 accgtttcta aatgttctct gagagttagt tcctcataga gacttggtgt ctcaggaata
    19621 aagcctattt tctttctata aagttcttgg ttggcttcaa gactaatgcc atcaatggta
    19681 atatctcctt gatagggtct caaaatacca ataatctcgt taatagtggt tgattttcct
    19741 gcaccattaa gcccaattaa tccaactaac tctccatcat ttacttcaaa agaaatattt
    19801 tttaaaactg ggatattaac atacccccct gtaacatttt caatttttaa cattgtcatt
    19861 ctctctatct ttgatatact ttattaacta ttataacaaa ataagaaagg gaaggtctat
    19921 tcttatggat aattgtatct tagaattccc ttatagtttt gaaaataact ttaaacctat
    19981 tttgtccgct tttacatcta attgaaatgc caagctaccc catgctccca ttatgtttac
    20041 aaaaagcacc tccatcactc atcggacaaa aaaagacacc tcaaaaaggt gtctttttag
    20101 tatcaatata ataaatgcaa agtaatcaga atattaggct ataaaattac caaagtcaat
    20161 tatcagaata aataactgta ttttttagta tattcgttga gtttaccttc taattttttt
    20221 agagtagttc tcttagaaat cccctcatca tatttgacac catccaagtc ccaaattagt
    20281 tctttatgta atttttctgc taaactagat ttcccttgcg gaaacttagc attaataaga
    20341 gccgaaattc tttggtattc ttgctctagt tctgaatact cactgttttt attttcaact
    20401 tcttttctta atatgtctaa tacataagaa tcttgtcttg atttaacctt tctgagtaag
    20461 gcaagttttt ctttaaaagg tatatttagg ttttttatac gctcctgaag ttctatctgg
    20521 ttattatagt tatattcctc agttaaatcc atattatttg atctatgtgg ctgatagtta
    20581 ttatatgtat ccgcagtaac gctattatat cctaaaaaga gagcagtaag agctgcagta
    20641 gataatatga ttttatttaa tttcaaaata gttttccttc tatgataaat ctcaattatc
    20701 atttttattt tatttatatt atttattata catttcatta gataaaaatg tcaatcatta
    20761 tatttaaatt tattttagat acttccaaaa ttctagaaat gtacttactc aataataaac
    20821 atcattaaaa attgcaatac cctttaggtc atgctaagat aagtatgatg gtaaatatag
    20881 atatcacaat caatacaggc tacttttatc cacctgtacg acttatgacc tatcaaacga
    20941 taaaaattta ccattcctta atagctaccc aataaatttt tcaacatgaa aggaattagt
    21001 tatggataat tgtatttttt gtaaaatcat cagtggagaa atctcttcat caaaagttta
    21061 tgaagatgat gaagtattag cttttctcga cattacacaa actacgactg ggcacaccct
    21121 tcttattcct aaaaaacatg ttcgaaatgt gttagagatg gatgaaaaaa ctgcacagat
    21181 aacctttgaa cgcttgccta aagtggcgcg tgctgtccaa gctgctacaa aagccaaagg
    21241 aatgaatatt attaacaaca atgaggaaat tgctggacaa actgtttttc atgcacacgt
    21301 ccaccttgtt ccccgctttg atgaatcaga cggtatcaaa attcactaca ctactcatga
    21361 acccgacttt gaggcacttg caaaattagc caaagaaatt agaaaagaga ttcaattatg
    21421 aaaataggtc gtttaattgc actaggttta gttagtttag gagcgtttga actttacaaa
    21481 aatagaaaaa ctatcaagga ttcctatcaa aatacaaaaa atgagacaga ttcagctaaa
    21541 ttaaaattag aacgtatcaa aaatgatctt gctattatta gtcaggaaaa agaaaaaatc
    21601 agattaattt ctcaagaact caatcataaa ttccaagtgt ttaataaaga tattcaacct
    21661 cgtttagaag aaattaatca acgcatggct aaatatcaag agaaagacga ataagccgac
    21721 ggtataaaaa tatctactct aaatataaaa agagcaaata atgctctttt ttatatttta
    21781 tggattagtt tgacttggta ctggttgcgt tcctgtgttt ggattaggcg ttgcagcccc
    21841 gttataatta ttaacacttc cacttgaatc agcctgacct gtgttagtgt tactgctata
    21901 gttactagca ggagtgctac tattatagta actttgatga gttgtttgat tagtctcaga
    21961 actataagta gtattaccac tgtaacttgg tggtgcttct gaataaggtg ttgtattata
    22021 attattctct tgtgttgatg aataagtaga agaatcatta ctagcagtag taccatagta
    22081 atcttcatat agaatcgcgc ttgtcttcag agttttacta cgctttttat ccagctcttt
    22141 cttaattcta ttttgaactg caagtagatg tttcttagtt aaaatttgat aagagccacc
    22201 atctgataga gtagcgtctt cacccttcaa ctgataagat ttaatatgtt ccaatgaatc
    22261 tttataagct aacaaattag gaatcgtttt tgatgatatc tcaatattag tttgcatgtt
    22321 attacttact gcggaaagaa tttttttgta tgaactaata ctatttaacg ccaatatttt
    22381 tttaaggact ttttgaatta cttcacgttg tcttttttga cgcccataat ctccctctgg
    22441 atcatcatag cgcatacgag aataaacaag tgcttgttct ccatttattt tatgtgtccc
    22501 tggttcaaca acagccttgt actctggttc attggcagca attgatattg gaaagtcaaa
    22561 tttattagtt actgttatac caccaacagc attgaccaaa tcaactaatc cttgcatatt
    22621 aatttgcata aagtaatcaa cattaatatc taataagtct tgaacagtca tcaatgccat
    22681 ttccgcacca ccagaagcat aggctgcatt tagctttgct tctacgccag tctgtccatt
    22741 atttttggga ccactcaatt taatcaatac gtcacgttct aagcttgtca tcgttgtttt
    22801 attagtttta ggatttatag tgactaagat catagaatcg ctatttcctg accacttaga
    22861 ttttcgatgc tctgaacctg tgtccacacc cattaatagt attgaaaacg gctttgtttc
    22921 ttcaatagca tgactttttg atttagctag cttaaaatct ttaaaagtct tagacaattc
    22981 attagtcgaa aaattataag ctgaggcaac atagactccc aatgcaatta ctgttgttaa
    23041 aataattgca gaaaacatta aggttatttt tttccaaatt ttcatgtata ttcctaatct
    23101 atcaacttac tcattctgta aatgtctaga aattctccat tttctctttt aatacctctt
    23161 gtttgaagcc catctatttc aaaactgaat ttttggtaga ggtgaatagc acgttcatta
    23221 cgtccttgga ctgatagttc taagcggcgt gtaatatcgt tatctgacgc ccattctatt
    23281 gcttcttcca tgagaatatg accaatacca tacccccaat aatccttctg aacagcaata
    23341 aaaagatccc caatgtgttc aatttcaata tcagactgac taatgattgt tatcaagccg
    23401 atcactttat tttttaattt agcaatcaaa caaatgcaat ttttcgttat taaggtattt
    23461 tcaataaacg tttccatttc tggcaaagat agttgattgc tcaccgtatg caaaataaaa
    23521 tctgtttcct cagtaacagt atttaaaaat tcaagaagcg cagatgcttc ctctctcgaa
    23581 gcttctataa aatcaacagc aaattgttta gacatcttga atcgcctcaa taatttctct
    23641 tgcgcgatgc ccataagctt ctaactgaac ttgacgtcct tgattgctcc tcgtaataac
    23701 aatttctaag tagttattga taaggttatc actaagcaac tctccccatt caataactgt
    23761 tactccttga ccaaacaaga agtcatctaa gtcaatagaa tcaggatcat ctccaatacg
    23821 ataaacatcc aaatggtaaa gtggtacacg cccttcatat tctctaacaa tagtgtaagt
    23881 tgggctctta atcatttgtt ttatgtctaa tccctttgca ataccttttg taagagttgt
    23941 tttgcctgca cctaaattac cagtcaataa tacgatatct cctgacttta aaacagtacc
    24001 cagtttttgc cctagggcaa taagctcttc ttcattttga gtataaaaca tagagatatt
    24061 ataccagaaa tatctctgtc ttccaacagt ttgctgattc tgacagcccc atagcatata
    24121 tttcttcact tttcttaaga aaaaaggaaa ccaggctaca aatctgattt ccttttatct
    24181 tatttatcaa actataatat tacaaaatag ccaagctgat aaagtttaga ataaataata
    24241 tatctaaaat ccacagtacc acatgaatat cttttgcttg tcctttgatg actttagcta
    24301 gtgtataggt caagaaacca gctgcaatac cataagtaat gctataagta aaccccataa
    24361 aaagagaagt aaagaaagct gggatagctt ctgacatatc atcccatttg atatctttta
    24421 aatttgaaag catcataata ccaacaataa ccaatattgg tgcagtagct tgactcggaa
    24481 cgatagatac tagtggacta aaaaaactag atatagcaaa caaaccagca actactaagg
    24541 ctgtcaatcc tgtacgacca ccagctccga ttcctgctgc tgactcaaca taggttgtaa
    24601 cgttagatgt tccacaaata gcaccaaaag ttgtcccaat taagtctgaa tagagtgctt
    24661 tgtctaaact cttagattca tgattatcac cagtcgttgc taagatacca actttttctc
    24721 cggtaccaat gagcgttcca atggtatcaa aaatatcagt cagtgaaaaa gcaagaatgg
    24781 ccattaatac acttggtaga cgtgaaggat tagaaaataa agaaattaat cctttctctc
    24841 ctaatgcaac tccaaatact tgcttcaaat ctctaaaaga ggccgagagg ttagttgctt
    24901 cccaattaat agcatctaac ttaaccactc ccattaaaat acctaagagg gtcgttgtta
    24961 atatcgatag aataatccct ccacggatac cttttacaat aaagaagatg gtaatgctta
    25021 aaccaatcaa agataataaa attgctggat tatcaaacga cactagccct ggcgttgctg
    25081 aagaatttgc tgtaatagta gccaatcctt tcgcagcacc ctttccaaca acatcatacg
    25141 tcccaggatc aattgaaaat ttcaaaaaac cagcattttt aatcccaacg tatgctaaaa
    25201 aagttccgat tcctgctgta atggctgatt ttaaagttgt tgggatagac tcaataatca
    25261 tctttctaac tttcgttaaa gtaataatca gagaaatcaa accacaaata aataccatag
    25321 ctaaggcttc ttgccatgta tatcccaatg caaagacaac tgtatatgta aagaaagcat
    25381 ttaatcccat cccaggagct tgtgcatagg gaaggttagc atagaaagcc ataacagatg
    25441 tagctacaac ggcaccaata atcgtcgcta agaaaactcc ttgtgctggc atcccagtct
    25501 gagataaaat agctggattt acaaacaaaa tgtacgacat tgcaaagaaa gtcgttagac
    25561 cagcagtgat ttcagtccga attgtcgtcc cgtgttcctt tagcttaaaa aacttttcca
    25621 ttttaaaatc ggattctcct ttattttttg ttattttaca cttttaaaaa aattaatcgt
    25681 tcgtatattt acgaactatt aatatatatt aacaaaaaat aattcacttt tcaagctttt
    25741 tccctgcaga ataatccata aaaataagac acaatacctg taaaaatgta aatcctaaaa
    25801 taaagtggaa tgaatcccta ctcggaaccg ctaaaagttc gttaattacc acttgtggct
    25861 tcgttaagat atcccatgaa tttagacggg cataacgacc aatatgaata ccaaaacttg
    25921 aaacaaatga tacaattcca ataattcccc atcttaaaaa tatattgcta attctaaatt
    25981 tacgcataat cacagaaaaa ctttcgatac ctgataaaaa accaaacaag atactagata
    26041 catacaagat ataaagtatt aaatttgtct tattataaag aacgtctccg acaaaatgca
    26101 tatgaatgat atccgtcaac atataaaagg tattaggata gaaaaaaaac caagcaagtg
    26161 caaaaagacc gataagaatt tttttcttaa ataaactagt aaggtatgca aaatctaacg
    26221 ctattagggc tagagtcata ttccaaatca aatttggtcc ctgcaaatga taatatttaa
    26281 tcccaaaaga aataacaata aaaaataaat gtatcgcaaa tagttttact ttcattctaa
    26341 cctccaatta atatctcatt atacaacata ttattaaatt aaggttaaat aaacttcaaa
    26401 cctaaaagac taaccctgtt gtggttagtc ttccattaat attccccaaa gtaataattg
    26461 tactaaaaaa aggcaagaca aggctttaaa atcaaggtta gccatattag tctgtattcc
    26521 attaaaaaat gctgacggat ttaaaatgat tccttctaaa cctaatgatc ctgatttttc
    26581 taagcaaaag gcaaaactac taaaaagcat tacaggtata acagttgcta attgatacct
    26641 tttactaata tcatatgcat ttaccaatag cttcaaactt agtaaagctg aagagataat
    26701 aaaaagaatt gcaagaaggt atgccatata ttgcataaag tgattagact gccaaatgtc
    26761 ttttgttgac cactctaagc tcactaaact aagaaaaatc cctaaactct gtgggtaaaa
    26821 aaataaaagg cctaaacttg ctaaaagacg aataaaaata ttacgactat aataagcaat
    26881 gaatcccatc tctaacgaca agactgatgc caatagaatt accatcaaat gatcaatttt
    26941 tacatgataa taccatatcc ctaaaatact gactatggca aaaagatgac tccaaattaa
    27001 ctttttcata atccaccaac ttccatattt ccacagttat tatatcatat ttctactgtc
    27061 tctttttatt gaaatctatt tttgatataa tgtcagcatg acaaagaaaa ttatcgccgt
    27121 agatttagac ggtactctat tacataataa taacactatt tctgactaca ccgctgatac
    27181 acttcgaaaa gttcaagctc aaggacacaa agtaattatt acaaccggac gcccataccg
    27241 tatggcactc gctcattatt tacgcctaga tttaaaaaca ccaatgataa attttaacgg
    27301 tgctttaact catatccccg aaaaaaagtg ggcgtttgag catagtgcaa cgattgataa
    27361 aaaattactc cttgaaacct taaatttatc agatgctatc caagccgatt ttattgctag
    27421 cgagtaccgt aaaaattttt acattactat ggataatcgt gataaaatta acccacaatt
    27481 attcggtgtc aatgaaatta ctgataaaat ggcattagat gttactaaaa tcacacgcaa
    27541 tccaaatgct ctattgatgc aaacacgtca taaagataag tatgagttag ctaaagagtt
    27601 acgacaacat tttaatcacg aactagaagt agattcttgg ggtggtccat tgaatatact
    27661 cgaattttca cctaaaggtg ttaacaaagc ttatgctctc aaacatttat taaaatcact
    27721 caatctttca caagaaaatt tgattgcctt tggagatgaa cacaatgaca ccgagatgct
    27781 ggcctttgca catacaggat atgctatgaa aaatgctagt ccaactctac ttccatatgc
    27841 cgaccaacaa atccaatgga caaacgaaga agatggtgtt gctaaaactc tagaaaaatt
    27901 attattgtag tattttcaaa aacgatctct attgaaacag aattctaata gaaatcgttt
    27961 tttaggtttt ctactatatt attaacaaaa aaagttacca ctctcttccc ccattataaa
    28021 aggtatttgt gaaaaaaata tctttttttt aatttttatt agaaatagta ttgatttttt
    28081 tctgaaaacg ttttaaaata tatctgttct ataatttcgt tgaaatgaaa aagaatggcc
    28141 aattttacga ttggaaggag attattcttc tttatgtcaa cgaaaaaatt ttttaaggag
    28201 gaagaacaag aatgggtatc ggtattatta ttgccagcca tggtaaattt gctgaaggta
    28261 ttcatcaatc aggttcaatg atctttggtg aacaagaaaa agttcaagtt gtaactttca
    28321 tgccaaatga aggaccagat gatttgtatg gacacttcaa caatgctatc gcacaatttg
    28381 atgctgatga tgaggtgctt gtgttagctg acctttggag tgggtcacca tttaaccaag
    28441 caagccgtgt tatgggagaa aatcctgaac gtaaaatggc tatcatcact ggtctcaatt
    28501 tgccaatgct tatccaagcc tatactgagc gtatgatgga cgctaatgca ggtgttgaac
    28561 aagttgccgc aaatattatt aaagagtcta aggaaggtat caaagccctt ccagaagaac
    28621 ttaatcctgt tgtagaagct actcctgtag caggtgttcc tgcagatgtt cctgcggaag
    28681 ttaaacaaag tggatctatc cctgaaggaa ctgttattgg agatggcaag cttaaaatta
    28741 atcttgctcg tattgacaca cgtcttttac atggtcaagt tgcaactgca tggactccag
    28801 catcaaaagc aaaccgtatt atcgttgctt ctgatgaagt ttctaaagat gaacttcgta
    28861 aacaattgat taagcaagct gctcctggag gagtcaaagc aaacgttgtc ccaattagta
    28921 aattaattga agtagctaaa gacccacgtt tcggaaacac acgtgccttg atcttatttg
    28981 aaactgtaca agatgcactc cgtgcagttg aaggtggtgt ggaaatccct gaattgaacg
    29041 ttggttcaat ggctcactca actggtaaaa caatggtaaa caacgttctt tcaatggata
    29101 aagacgatgt tgctgccttt gaaaaattac gtgacttagg tgtatcattt gatgtacgta
    29161 aagtaccaaa tgacgctaaa aagaatttgt ttgatttaat caacaaagca aacgtaaaat
    29221 aacttattat ctctgaaaac gaaaggatat caaaatggcg gatatttcaa ttatctcaat
    29281 gattttggtc gttatcgttg ccttctttgc tggtcttgaa ggtatcctcg accaattcca
    29341 aatccatcaa cctcttgttg cttgtacttt aatcggtctt gtaactggtc acctcgaagc
    29401 gggtattatt cttggtggta gtctccaaat gattgctctt gcttgggcaa atatcggagc
    29461 tgccgtagca ccagacgctg cacttgcctc tgttgctgct gctattattt tggttaaaag
    29521 tggcgacttc actcaaaaag gtatcaactt tgccttctct actgctgtac ctctagctat
    29581 tgctggactt ttccttacaa tgattgttcg tacaatctca actgcacttg ttcacgctgg
    29641 tgacaaagct gcttctgaag gaaacttcgc agcaattgaa cgttttcact ttattgccct
    29701 ccttctccaa ggattacgta ttgcattccc agctgcgctt cttttagcta ttccatcttc
    29761 ttcagtacaa agtattcttg aagcaatgcc tgattggtta aatggtggta tgcaagttgg
    29821 tggtgccatg gttgttgccg ttggttacgc tatggttatc aatatgatgg ctactcgcga
    29881 agtttggcct ttctttgctc ttggatttgc tctagcagct ttgaaccagt taactcttat
    29941 tgcaatgggt actattggtg ttgctatcgc cctcatctac atcagtttat caaaaatggg
    30001 tggaagcaaa ggtacttcta acgcaggttc taacgatcct atcggcgata tcttagagga
    30061 ctactagaaa ggagctaaac aatgactgaa caaattaaat tatcaaaatc agatcgtcaa
    30121 aaagtttggt ggcgttcaca attccttcaa ggttcttgga actatgagcg tatgcaaaat
    30181 atggggtggg cctacgctct tatcccagca ttgaaaaaac tttacactac taaagaagat
    30241 cgtgctgctg cgcttgagcg ccatatggaa ttctttaaca cacatccata cgttgctgct
    30301 ccaattatcg gtgtaaccct tgctcttgag gaagaaaaag caagcggtac acctgtcgaa
    30361 gacaaagcaa ttcaaggggt taaaatcggt atgatgggac ctcttgctgg tattggtgat
    30421 ccagtattct ggtttactgt acgtcctatc ttaggtgctc ttggtgcttc acttgcctct
    30481 gctggtaata tcctcggacc aatcatcttc tttgttggtt ggaacctcat tcgtatgtca
    30541 ttcttatggt atacacaaga gttaggttac aaatcaggta aagaaattac taaagatatg
    30601 tctggcggta tcctccaaga tattactaaa ggtgcttcaa tccttggtat gttcattctt
    30661 gctgtccttg ttaagcgttg ggtagcaatt aactttactg tagaccttcc taaaaagaca
    30721 ttgtcagaag gggcatacat caatttccct aaagaccatg ttagtggaca acaattgcat
    30781 gatatcctag gacaagttca gagtggctta agccttgata aaatgcaacc acaaacactt
    30841 caaggacaac ttgactcact tattcctggt ttagctggat tacttcttac attcttctgt
    30901 atgtggttac ttaagaagaa agtatcacca attacaatta tcatcggttt gtttatcgtt
    30961 ggtattctag ctcgccttgc aggtgtgatg taataaaact aagagatggt tacaatagta
    31021 ttccatctct tatattttat taccaaaaat ccacttagag tcccactatt atgatataat
    31081 caaactacta taaattaaaa agggattagt catggcacaa tcattaaaca aaactgttga
    31141 atttcaaaca acaggagttt cgtatttagg tatggggaat aaagttggaa aatttttagt
    31201 tggtgaccag gcattagaat tctataacga taaaaatgtc aacgactata ttcaaattcc
    31261 atggacatca atcaatcaaa ttggagctaa tgttagtcga aaaaaaatta gtcgtcactt
    31321 tgaggtcttt acagaccaag gaaaattttt atttgcctct aaagactctg gaacgatatt
    31381 aaagcatgcg agacgacata ttggagacga taaagttgtt aagcttccta ccctcatcca
    31441 gaccatttta aaaatattca ag
//
LOCUS       DATRME010000015        29084 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRME010000015 DATRME010000000
VERSION     DATRME010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29084
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..292)
                     /locus_tag="VBC23_000709"
     CDS             complement(<1..292)
                     /locus_tag="VBC23_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842675.1"
                     /translation="MIQDILKSVSREDSNTLETDTLAIKDNAIIVKNSSIQITSISMV
                     SRSNFSSPITLLDYVILFVLFIIGLIPPFIGLIFLVGYAYLVYKKHKQHLNSK"
     gene            complement(424..1668)
                     /locus_tag="VBC23_000710"
     CDS             complement(424..1668)
                     /locus_tag="VBC23_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO6842676.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKAEAFEYRQSLMTK"
     gene            complement(1678..2751)
                     /gene="purK"
                     /locus_tag="VBC23_000711"
     CDS             complement(1678..2751)
                     /gene="purK"
                     /locus_tag="VBC23_000711"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6842677.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            complement(2738..3226)
                     /gene="purE"
                     /locus_tag="VBC23_000712"
     CDS             complement(2738..3226)
                     /gene="purE"
                     /locus_tag="VBC23_000712"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6842678.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(3512..4774)
                     /gene="purD"
                     /locus_tag="VBC23_000713"
     CDS             complement(3512..4774)
                     /gene="purD"
                     /locus_tag="VBC23_000713"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6842679.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            5056..5859
                     /locus_tag="VBC23_000714"
     CDS             5056..5859
                     /locus_tag="VBC23_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6842680.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(5882..6859)
                     /locus_tag="VBC23_000715"
     CDS             complement(5882..6859)
                     /locus_tag="VBC23_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6842681.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(6867..7748)
                     /locus_tag="VBC23_000716"
     CDS             complement(6867..7748)
                     /locus_tag="VBC23_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6842682.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(7765..8682)
                     /locus_tag="VBC23_000717"
     CDS             complement(7765..8682)
                     /locus_tag="VBC23_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6842683.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(8679..9341)
                     /locus_tag="VBC23_000718"
     CDS             complement(8679..9341)
                     /locus_tag="VBC23_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6842684.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(9361..9804)
                     /locus_tag="VBC23_000719"
     CDS             complement(9361..9804)
                     /locus_tag="VBC23_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6842685.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(9817..10647)
                     /locus_tag="VBC23_000720"
     CDS             complement(9817..10647)
                     /locus_tag="VBC23_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6842686.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENISTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(10657..11544)
                     /locus_tag="VBC23_000721"
     CDS             complement(10657..11544)
                     /locus_tag="VBC23_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6842687.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(11632..12948)
                     /locus_tag="VBC23_000722"
     CDS             complement(11632..12948)
                     /locus_tag="VBC23_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6842688.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGSEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(12995..13693)
                     /locus_tag="VBC23_000723"
     CDS             complement(12995..13693)
                     /locus_tag="VBC23_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6842689.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(13940..15244)
                     /locus_tag="VBC23_000724"
     CDS             complement(13940..15244)
                     /locus_tag="VBC23_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6842690.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(15391..16290)
                     /locus_tag="VBC23_000725"
     CDS             complement(15391..16290)
                     /locus_tag="VBC23_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6842691.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGTATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(16483..18030)
                     /gene="purH"
                     /locus_tag="VBC23_000726"
     CDS             complement(16483..18030)
                     /gene="purH"
                     /locus_tag="VBC23_000726"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6842692.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(18050..18802)
                     /locus_tag="VBC23_000727"
     CDS             complement(18050..18802)
                     /locus_tag="VBC23_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6842693.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(18825..19373)
                     /gene="purN"
                     /locus_tag="VBC23_000728"
     CDS             complement(18825..19373)
                     /gene="purN"
                     /locus_tag="VBC23_000728"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6842694.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(19540..20562)
                     /locus_tag="VBC23_000729"
     CDS             complement(19540..20562)
                     /locus_tag="VBC23_000729"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6842695.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLLDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(20590..22044)
                     /locus_tag="VBC23_000730"
     CDS             complement(20590..22044)
                     /locus_tag="VBC23_000730"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6842696.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(22278..26003)
                     /locus_tag="VBC23_000731"
     CDS             complement(22278..26003)
                     /locus_tag="VBC23_000731"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6842697.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(26126..26830)
                     /locus_tag="VBC23_000732"
     CDS             complement(26126..26830)
                     /locus_tag="VBC23_000732"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6842698.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(26954..27193)
                     /locus_tag="VBC23_000733"
     CDS             complement(26954..27193)
                     /locus_tag="VBC23_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6842699.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(27204..28196)
                     /gene="plsX"
                     /locus_tag="VBC23_000734"
     CDS             complement(27204..28196)
                     /gene="plsX"
                     /locus_tag="VBC23_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6842700.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(28274..>29084)
                     /locus_tag="VBC23_000735"
     CDS             complement(28274..>29084)
                     /locus_tag="VBC23_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6842701.1"
                     /translation="NWFVPVIGAVLIQVIGIIVSRRLVVKLLPDDYKFNLYIALLLFI
                     PILASILLFLIYFQKRDFTSLGFTKDNNVLTTISGFITGSLLMFFIYICHWLSGFVEI
                     SSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYVMTTVTKFKGSFAGVLCNSMLFSFIH
                     FRNYGITAIALFNLFLLGIIFSILFNMTKNILFVTGVHTAWNFTMGCVLGNKVSGGYS
                     PVSLFRITENSSFAIWSGGDFGFEGGVLCSLILVGCLAYFSLERVKVSVRY"
BASE COUNT         8768 a         6286 c         5057 g         8973 t
ORIGIN      
        1 attttgaatt taaatgttgc ttatgctttt tataaactag gtaagcatag cctaccaaaa
       61 atataagtcc tataaaagga ggaatcagtc ctataataaa taatacaaat aaaatcacat
      121 aatctagcaa cgttattgga gatgaaaaat tacttctact taccattgag atagacgtta
      181 tctgaatact gctatttttt actattattg cattatcttt gattgctaat gtatctgtct
      241 ctaatgtatt agaatcttct ctacttacac tttttagaat atcttgaatc aattcttatt
      301 tctcctttta ttttttgaaa ttaatttttg gaatgaaaga tgcctacaaa taagtcacaa
      361 ttatcttttc aacaaagcta caactatatt ctgttatata caaatacagt cttttctata
      421 taattatttt gtcatcagac tttgtctata ttcaaatgct tctgcttttt cagtcaactc
      481 gatagcaagt tctttatgct cctctaacac tgctttgatg tcagaaaatg ggactttaaa
      541 atactctttt cgcccattaa ctttattaat tttatttttt tcaaatctgt tatgtaactc
      601 tttttctaat gaaaaagctt cctcactaaa aattaaggca tgtacatcaa attgaaatgg
      661 aacagatgca ctccctaatt cgcgaatacg ttccatagga tctagacgcc ttgtaacacc
      721 aatcttgtaa acatcttctc caaatgcacc aatatttgaa atgatataaa catatccagc
      781 agttgcatgc ccttctcgat aatcaatatc ttcttctaac tcatctagtt ctgataattt
      841 atcttggtat tctgctaatt gaagatttaa tttttccttt tcttcctcat tagctgtttt
      901 aagtaattct tcaacgttat ctaacatctg tttaaagtgt tttctatctt tttcaatttg
      961 ttttctctta gcagctaatt ctgcttgaag tttcttatct tctctttcct tcgcacgttg
     1021 ttcacgaaga agttcttttt cttctaattt ctgaagttca tattctactg ctaattgtaa
     1081 ctctgaaatt tttagctcaa gatagtcatt agatagacat accaaatttc ttgaatacat
     1141 cttattaagc tgattaaatg atttaacaac tgcatctact tttttactta gattagtagc
     1201 cgttaccttt accaggtgag catcaacttc tccattaaaa cctcttatca tcgctttaat
     1261 aagttgattt tgcattgctc gtccttttga tttactgtta tccagcaaca taggtcttac
     1321 aataacaccc gcttttttat cttttactaa ctgtttttgc ctctttctca gagtttccaa
     1381 ttcttcttta tacttagtac tatctgagaa cttatatttt ctctcaaaaa atccgtactc
     1441 ttgaagatct aaatcatcat taagaactat tacctctttt tgtaaagctt taagttctga
     1501 ttggcgtttc ttaagttcat catcaagttt taaaatatct ttagcaacct ttatctgcct
     1561 tgttaaatca tccaaaatag attgtttatc ttttaactta ctttctaaac tactgatttt
     1621 agcttccaaa gcctcatctt taggctttcc gaatccaaat agacccattt attcccccta
     1681 aaactcctcc acctcatccg catcttccac aaacaccgtt acatgtccca ttttgcggtt
     1741 atgtttcgct tctagtttac catacatgtg gaggtgagcg ctagggtttt gggcaacata
     1801 gtcaatcgcc tgctggacat gttgtccaag gacattaagc ataacggctg ggtcatggag
     1861 cttgattggt gggagaggtt gccccagtac acccaagatg tgggtgtcaa actgtgaaaa
     1921 gtcacaggct tcgatagaat agtgccctga gttgtggggg cgtggggcga tttcattgac
     1981 gatgatatca tctgcggtca caaacatttc cacacagagg gttcctgata gctggagttc
     2041 cttggcaatc tgtaccgcca ttttcttagc cttgtcagct agttggtctg agatgcgagc
     2101 tggtacgata gtttttgata ggatattgtt gcggtggata ttttcctgaa cgggaaagac
     2161 cgtcacatcc ttgccatttc cagacacgat gactgagatt tcaaggtcaa agtcgacaaa
     2221 ctcttccaag acacactcag ctgagttggc taattgttgc gcctctggca ggtcttctgc
     2281 tgagcggata accttttgcc catgaccgtc ataaccacct gtcgctgtct tgaggacata
     2341 ggtcttggtc aagtcaagcc cctctaggtc aaggctagac gtcaccacct tatagggagc
     2401 gacagtcact ccagccttat ttgccagaaa gtctttttca aagatacggt tttgagaaat
     2461 gcggagcaga tcagtcccct gcggtagctg accagctgac acaacggcat ccagaccatc
     2521 ggcatcaaca ttttcaaact cataggtcaa aacatcacaa cgcgcagcta attgtcccaa
     2581 agcctcaacg tcatcgtaag gcgccacaat cacctcgcta acgcgggagg cagggcagtc
     2641 gctagcagga tccagcgtaa tgaccttgtg gcccatgtag atagccgcaa tcgccatcat
     2701 ctgccccagc tgaccaccac cgataatccc aatggtctta aatgagttca ttgcttgact
     2761 cctctgcgat ttttccttgt tcctcatgga aatgagctag cgcatcagcc agattttggt
     2821 cctcgataga gagaatacgg agggctgtta gggcagcgtt agtcgctccc gcttctccga
     2881 tagccatggt tgccacaggc acaccgccag gcatctgcac aatggaatag agcgaatcca
     2941 agcctgaaag ggcacgtgat ttgacaggca caccgataac aggcagggtt gttttagctg
     3001 ctaccatacc aggcaagtgg gctgccccac ctgctccagc aatgatgatt ttgataccac
     3061 gaccacgcgc ctcctcagca tgcttgaaca tgaggtctgg cgtacggtgg gcagagacga
     3121 ctttcttctc gtaagcaatg ccaaagttat ctagaacttc agccgttttt tgcatggttg
     3181 tccagtcgga tttggaaccc atgataatag aaataattgg ttgcataata ttaagataca
     3241 aagggacgtt tcgacttgtc gaaaattttc gtagaaaaat atgcggtaag tccgaaatct
     3301 ttgatttcga agacgcaccc acgtcagggc gactaggtgc tttagcagca gacgcctacg
     3361 acgcaaaaat ctgacgaaga tgctttagca tctaggaaga tttatctttt tcactagaaa
     3421 attagtcccg tgttcagttc ctttcttctt tctttttgga gcgaccttcg gtcgctgatt
     3481 gctgacgcta atatttgaat gagtagtctt attacttaat agctttgctt ccgatgtcgt
     3541 ttcggtagaa gaggcctgtg gtgtcttgtt gggctagttg ggtatagatt ttgtcctgcc
     3601 ctgctttgac gctgtcttct gtggtgacaa gcatatagac tcgtccaccg tttgagagca
     3661 gtgctttgcc attttccgca aacttagctc cggcatagta ggtgatgatg tcgccgtcgg
     3721 tcttttcagg cagtggcaca cctttctcgt aatcgagcgg atagccttct gaggcaacga
     3781 caacgcccaa agtcacgccg tccttctgcc aagtgatgta aggctcgatg cccatcatga
     3841 tgtcgtcgat gttctgagca aaatcagaag tcaggcgagg gaggataatc tgagtttctg
     3901 ggtcaccgaa acgtgagttg aactcgataa ccttcggacc atcagccgtc aggataagcc
     3961 ccgcatagag gacgcctaga taaggacgcc cttcagtaat cataccctca aggataggct
     4021 taacgatggt ttcaaccgcc gtatccacta cgctctgagg caggtgggga actggcgcat
     4081 aggcacccat accgccagtg tttggtccca tatcaccgtc ataggcacac ttgtgatcct
     4141 gagctgtcgg catgatgtaa aacttgtcgc cattagcgaa ggcaaagagg gagaactctt
     4201 caccatccaa aaattcctcg ataaccacac gcgctcctga gtcgccaaac ttgttgtcca
     4261 aaagcatctc ttgcgccgcc tctaccgcct gctcaacggt ttcagccacg accacgccct
     4321 tgcctaacgc caagccatca gccttgacca cgattggtgc accctgctct tcgatgtagg
     4381 ctttagcttt ttcaaaatct gaatatgtgc cataggctgc tgttggaacg ttgtatttaa
     4441 ccatgatttc cttagcaaag tcttttgacc actctagctc cgctgctgcc tttgttggcc
     4501 caaaagctct aagtccagca ctgttgaaat catcaacgat accagccgct agcgcatcat
     4561 caggaccaat aaaagtccaa gcaatctcat tctccttagc aaagtcaatc agcctagaat
     4621 gttcggaaat tacgatattt accaagccta gaccatccaa ggtcatacca tcattaccag
     4681 gtgccacaaa aacctgatct acacccttag acactaacaa cttcttagca atcgcatgct
     4741 cacgaccacc agaaccaaca acaagcaatt tcatatcaga ataactccta ttaaataata
     4801 taaaaagaaa tttgcaataa aaacgtatta tttataaaat tataacacat ttgttcgctt
     4861 ttaagttttt tgttgcatct tttttgaaaa attaataaca taatatatac ttcacatata
     4921 ttatgttatt aatccttcat attgctattt aaaacttatc attgctgttt cttagaactt
     4981 agtttggccc ctcgctaact ttatgttata ctgaatatgt taagcgcttt cttaaagagg
     5041 agaggaccgt gcattatgac tatttatgac caaattgagt ctgctcttga tttaatgact
     5101 gatttagaac atgaaattgc ttgttatttt atggggcagc ccatttcaaa agacgctctt
     5161 gcttcttcca ttgtcactaa gcaactacac atttcacaag cagccttaac gcgttttgct
     5221 aaaaaatgtg ggtttaaagg ctatcgtgaa tttgtttttg aatatttaaa aagccatgag
     5281 accatcagtc aacaattata cggtcttcaa aatgacaaca ctaagaaagt atttatgaat
     5341 tatcaagaga tggtttctaa atccgctgat attattgatg aagaacagct tctagaagta
     5401 tcacatatga ttgagcaagc tgatcgtgtt tatttttatg gcaaagggag ctcatcgcta
     5461 gtagctaaag aatttaaaat acgccttatg aggctgggag tgatttgtga ggctctggat
     5521 gatacagata gtttctcatg gactaatagt attgttaatg atagatgcct cgtcattgcc
     5581 ttctcacttt ctgggaacac caactctgtt atcggtgcct taaaaatcgc aagttgtcac
     5641 ggagctaaaa ccgttctatt tacaaaacaa cctcacacaa ttgactatgc ttttgacaag
     5701 attattcagg tggcctctgc taggcattta gactacggca atagaatttc gcctcagatc
     5761 ccaatgttaa tcatggtaga tattatctat gcacaatttt tagatattaa taaaatagaa
     5821 aaagagcgaa tttttagaga aaccattatt caacgctaaa tgaaaatcga gtagaaataa
     5881 tctactcgat tttttgcttt gtaaattgac ttcctgtcag ttgatcaaaa atatagtctt
     5941 ttacttgtac tgtcattgga tcatgtccat agtctggtaa taaaagatgc tttttagttg
     6001 atgtcaaacg gttaaaaatg gcaaattgac ttgatggagg acaaatatca tctttcaagg
     6061 ctgttagtaa aacaacgggg caagaaatcc tatgtgcaaa atttttaaca tcaatataag
     6121 ccaaagtttt taacacatta ttttctgttt tatgaaaagg atcactatat ttaaaatagc
     6181 ggaagagttc gtcataaggc tcactgacat tccctaaatc taatactcgt ctaaagtctg
     6241 acaaaaatgg gtaaacagca acggtcttga cgattttggg attaagagct gcagctatca
     6301 aagcaagtgc tccaccttga gaccagccat aactataaag ttgattacta tcaacactct
     6361 ctaatgtagc aatgatatca atcaattgga aaacatcaag ataaatgtct ttataaaaaa
     6421 gatgatttgg tcctgagatc attcctcgca caatctggcc cttaactgta atgccatcaa
     6481 aatgcccctt gtcttgagac tgtccagctt gacctcgaac atccattgaa acaacgccgt
     6541 aacctgctgc tacataatta agttggtcac tccagtcagg actttggttt tggtaaccat
     6601 gaaaataaaa aactacagga tagggtttat ttgttttagg gaataaacat ttggcataga
     6661 caatagagtt attgctgcct ttaaaatgaa gatcataagc ttgtgcaaaa gtaatattga
     6721 aatctttttt gtctagacga tactcaatat tcccttgata tttcattgtt tgttttttcc
     6781 agaagtcatc aaaatcctcg ggaatttggt cttgacctag gtattctctc atgtcatcta
     6841 gcgacattgt ttcaatcata tacccgtcac cccctgtttt taaaatggta gtaagctcct
     6901 agcataccag cttggttttc gtgttgtgca aaaactatta ttgttttttc agcaagactt
     6961 gtcacaagat ttcgtttcaa agattcacta agtttatctt gtaaataatc tttttgagcc
     7021 atgattccac cacctaaaac aactttttca ggatttacca cataaaccat attagcgatt
     7081 ccttgaccaa ggtagtttat catacggtct atacttgcaa tacatttctc attgccttct
     7141 ttagcctcct gaaaaatacg acgtccatcc catcggttaa tttcatctcc atgcgctttg
     7201 gcaacatcag caatcagtgc agttgtagag gctaaatctt gaaaatcacc atctgaaagg
     7261 tgcatatagc caacttcaca agcagaatta ctaaagccat gaaaaactgt tttatcaata
     7321 atcagacaac caccaatacc tgttccaata gtaagacaaa gcgctattga actatctttg
     7381 gcactacctg agacagcttc cgctaatcct gcacaattaa catcattttc aatttctgtg
     7441 cgtacttgat aagtgtcttc taaaactttc ttaaattgtg tcccagcata gtttggaatt
     7501 tgaggaccag agtaaaatat gcatccttca tctggatcta ccatgccagc agatgagata
     7561 gcaattccat caatgctccc ttctgttaga tagttatcga taatttgaca aactttttgt
     7621 aaaatgcctg gaccaccttt gtaggcttct gtggcaagct cacttgcctc aacaatacaa
     7681 cctaaattat ccacaatgcc atgcttaatc attgtaccgc caatatcaat tgctactgtt
     7741 ctggtcataa ttatctacct cactttaaca aaactgtttt ttagcatgtc taatcatttc
     7801 tgctgcctct ttaataatag gtttatcttc ttctgttaca gatgctaaag gtaaccgaac
     7861 ggaacctata gttagttgct cattgatttc caatacagct ttgatcacag catacatatt
     7921 accatgaccc gaacaaagct ttgtgataat atcgttaatt gtaaattgaa gttcacgcgc
     7981 tttttctaaa tctttatcaa caattaactg atttaaagtc aagtataatt ctggcattac
     8041 gccataggta ccaccaatac ctgcagctgc tcccatgaga cgaccaccca aaaattgttc
     8101 atctggacca ttaaaaacaa tatgattttc tcctccaata gccacaaaat tctgaatgtc
     8161 ttgaactggc atggaagaat ttttaacacc aattacttgc ggattttgta acatttttcg
     8221 atataaatcc gatgtcaaag caacccctgc caattgagga atattgtaaa taataaaatc
     8281 agtttgtggt gctgcttgac taatagtatt ccaataatct gctatggcat actctggtaa
     8341 tcgaaaataa attggtggaa ttgctgcaat agcatcaaca cccatagctt cagcatgcat
     8401 agccaactcg acgctatcct tagtattatt acatgctaca tgcgctatca ctgttagttt
     8461 acccttagca acggacataa cattttccag tacaagttta cgatctgcta cactttggta
     8521 aatacattct cctgatgaac cattaacata aagtccttga acacctttat caataaaata
     8581 attggtcagg gcttttactc gttcgggaca aatatcaccc ttatcatcat agcaagcata
     8641 aaatgcagga ataattcctt gatatttttg taaatctttc attgataatc cccttgttgt
     8701 ttgctgaaat aggcaaattg tttttgtaaa ataataattt taaagtaagc aaaggcggta
     8761 taagcaaaaa taaatatagc tgacactgta agcataaata agagactaga ctgcaccata
     8821 ataaaccaag tcatgattaa taaaaacatc aatacaccag tccatttcaa gtttaaacat
     8881 gccattataa agctcttttt cacggtatct aaaagagaca tttcatagcg ggcagccata
     8941 ggaaataggt atatgctcgt catcacagtc aataacaaaa aggcgtaaca aattatcttc
     9001 aagaaaggca caatgaaacc tatttgataa tgaagaattg ttaaatcaaa tatgatcaca
     9061 accaaaatac ttagttcaac caaacctaaa agcatacctt ggaaccactc tttttttagg
     9121 tgttttatat acaatattaa aagatttgtc tgatcacctt ctaatttttg ccacaacgtt
     9181 cgatacaaac taattttagc agcaccaata gttaccaatg gtaggcaagt tatcataaag
     9241 agaaggttta gcaatgccag atcaaaaaca acattgcaac cttgcataaa ggggttgtta
     9301 agatcaaaaa tcgctgctat taattgattt gcttttttca caatatactc cctgtatttc
     9361 ttacgccatt aacactttca ataccccttt tttgacataa ttacctagac ttcccatacc
     9421 atttggctga tgagcttctt ctgggaaaaa gaaggctata taattatctc tcaaatcaaa
     9481 agaagtctct tctgagcact tgacaaatcc tatatcgcct aagtattcct ctacctctac
     9541 cttatcacct tttcccaatt tgataacttc atatccttca atgacataat gtatatcaag
     9601 gtatttttta tggtattcaa aaatatgatc aaatgactca ctaagttgat ttgattggac
     9661 catgagtatc acttctgggc taatatgata ggtgcctata tctaaattcc ttaaatcatg
     9721 acttagtaga taatcaatcg ctaaatctaa attaggatta atatctttat aatgcgttaa
     9781 attcaataaa tgatcgtaaa tcataataaa tcctacctat cctttaacag ctcccattgt
     9841 aatcccctga gtaaaggatt tttggaaaac taagaatact gtcacaatag gaacagcagc
     9901 taaggctgca cctgccataa ttaagccata gtttgttgcc atttcagcct gcatagtagc
     9961 taccccaaga gaaatggtca gattatttct agaagttagc ataactaatt gcataaaata
    10021 gtcattccaa gtattgataa atgtgaaaat agctagggca gcaaatccag gtttgacaat
    10081 tggaaatgct acattaataa atgttcgaat ctctccacaa ccatcaattt tagctgattc
    10141 cagtaattct gttgaaatat tttcagaaaa ctgtttcatc aagaagaccc caaaaggcca
    10201 accaacaaga ggtaaaataa ccgcccataa ggtatcatgt atccccataa aattgataat
    10261 tctaactaat ggcactaaaa caacttgttt aggtaaagcc atagcagcaa taaaaaggga
    10321 gaacaatatt ttttgaccgt aaaatcgttt cttagctaaa acatagcctg ccattgacga
    10381 tgtacagcaa actaggaaca ttgtcataat tgagataaag acactattcc aaagccatct
    10441 caaagctggg ttttgtacag ttaaagcttt aaaattttct aaggtcggtg ctttgggcca
    10501 ccattgtggg gggatgataa tagtatcagg ttgagattta aaagctcctg tcataatcca
    10561 ataaaatgga aagataaaaa gcactgtcaa aagacaaagg ataagagccg ttaaaaagtt
    10621 ataagcactg aatgttttct ttttcatgtt ttcctcctaa tattccacat catttcctaa
    10681 gatcttaaat tgagcaaaac ttattaaagc aatcattact gctaggaata ctcccatagt
    10741 attagcatag ccatattctg atagtttaaa ggctttttca taaagataat acatcaaagt
    10801 gcttgttgaa taatttggtc caccagatgt taggagctgg attaaggcaa aacattgaaa
    10861 tgaattaatt gttgttataa ctgcaatata gagtgtagtt ggtaagagac tcggccattt
    10921 aatttgccaa aaaacttgca tttcattggc tccatcaaca cgcgcagctt cgcaaagaga
    10981 attatcaata tttcccatcg ccgcaatata tagaataata ggttgaccaa ctgaggtcgt
    11041 tagcaaaata ataataatag caagtaatgc ccagtgctta tcaccaagcc agctaatatt
    11101 ctgttcaatg acatgacctg atttcaagat gtaatttaaa attccggaca tagggtcata
    11161 aatccatttc caaacaactg tgactgcaac actaccagtt actactggca agaagaagac
    11221 acaacggtag aaagagcgtg aaaaaacatt tttttcataa gtattggcag caacaaaaag
    11281 tgagaagaaa accactactg gcacagagcc aatcacaatg attactgtat ttatcaagga
    11341 tttcatgaaa atagaatcat gaaacatacg attgtaatta gctaacccaa taaaagtgaa
    11401 ctgtgtcata gaataattaa aaaaactagt gacaaagccc ataaccatcg gtgcaaaaac
    11461 aaaaattaag aaaaataaga gaataggcgc tagaaaagca taagctatca tcgtttccct
    11521 catctttaac ttattagtac gcatacggta ctctccttta catcttaact tgactaaaaa
    11581 ctcctcttct atcccaatat gtgattgacc atagaaaagg agtctcctta attatttagc
    11641 tgctttttta atggtatcat ttgctttttg agtaaagtct ttcaaagcat ctgctggttt
    11701 ttcatcacca ttggatacag attgaaccat tgggaaccat aaggttctca tttcagaaaa
    11761 tccatcgata gtgttgtaat atggtgaata atattgagtc cattttgaaa tcttcatcat
    11821 acgtttatca cctttataaa gatccccaaa tgatgttcta actgggaaag cacctgtacg
    11881 tataacatct tttggtcccc attttttatc atcagcaata aaagtgataa atttcttaga
    11941 ggcttttact ttgttttcat ctttattatt aaagaccgca aaaccattaa caaggtattc
    12001 taaatctggt tttccatctt ctgatgggaa tggcacttca aggtaatcca ctttacttga
    12061 ctctaataat tttgcttgag tttttggttg agctggagcc cataggatag tgaaagcagt
    12121 ttgtccattg gcgaagtttt gaatgtcagc tgagccatca tactgagacc cattcatcaa
    12181 gtagcctttc ttaatccatt caactatttt tttcattgat tttacagaat ttttagtgtc
    12241 agtggtatat tttgttactt ctttatctgt tattggagca ctataaagat tagcaaaaaa
    12301 tgcacgtggt ccttgatctc ctccttgccc gtttgcaaag aatgaacctg gtgtatagcc
    12361 tttatttttt agtgctttta gtactttttc aaaatcacta gtagtccaac cttcttttac
    12421 aagtttcaaa actcctgcat ctttaagcat ttttttattg aacgccatat aaaatggggc
    12481 agaacttatt ggatacatgt aggctttatc gccagactta gaagcttgaa tgatgttctt
    12541 attattgaca tccttaataa attggtctgt aaataaatca ttcaaatctg ctaatttacc
    12601 atttttacca tattgaataa ttcgccctgg tgcatcaaaa agcacatcag gtgctgtccc
    12661 tgcttcaatt gctgtagtga ttttttcaga tccagatgtg aaatcaattg tctctagttt
    12721 tacttttata ttaggatttt tcttttcaaa agccttgatg acttttttct cataagtacc
    12781 tactccatcc ttagcctttt cttgagtaaa ggttggaaaa gcccaccacg taatttcgac
    12841 tttatccttc tcggcagcca taacagattg actactgtct acacaagcaa acattgataa
    12901 tgctgcagct ccgaggcaaa aaccaatcac actttttttg atactcataa ctattcccct
    12961 tttttatttt tattttgatt tttgagtaac acttttaact aagtcctgag atgaaacgct
    13021 ccgctatttc ttttggtcta gtgatagcac ccccaactac aattcctgca acacctatat
    13081 gattaatttc attagcctgc ttaggagtat gaattttacc ttccgcaatc acatctatac
    13141 cggcttgaca aagcttatta aggagttcta tatccggtcc ttcttcttgg cggctgtaat
    13201 ctgtgtatcc cgatagagtt gtacccacaa aatcaactcc tgcttcaaaa gcatttttgc
    13261 cttcttcaaa agtacttata tcagccatta acaactgttt aggatatttc ctttttatct
    13321 tttgaataaa ctcagctaca ctcaaaccat catgacgctc tctaagtgta caatctaagg
    13381 ctattactgc aatatctaaa gtagctaatt gatccacctc tgtcatcgta gcagtgataa
    13441 atggttcttg tggaggatat tcacgtttaa taatgccgat gataggtaaa ttagtaactt
    13501 cttgaatttc cttaatgtcg cggacactat tggctcttat accaaccgct cctgcttctt
    13561 gagctgccaa agctaaaaga ggcataacac ctccactttc agtataaaga ggctccccag
    13621 gcaaagcttg acatgacaca ataatgccat tttttatttg ctttttaaaa gcttctttac
    13681 ttagatgtgg catattctca cttccttatc ttttttaatt ttagatatct acaaacctta
    13741 ttatatctat ttttaaagcg ttttcaataa tctttacaga cttagatttt agttttaacg
    13801 ttttgattta gtcacggtta acgtgaaagt agtttcagac ttatagctta atctcctcaa
    13861 tttaataaaa attatataag aaccttgaaa gtctattcaa ggcatataaa aaagaagcca
    13921 aatagcttcc ttttttatat tatttgttaa atgatacgtg aacgtggtca tagtggttgg
    13981 cagtaacgcc accacgatct ggcattgcat tccaagtatt agcaggtcca taaatactat
    14041 ttgtatttga ataaaacttt tgttgccaga taacatatga aatgttattt gctgccatat
    14101 tttgtgtaga gtactgtgca acttcattac caagtgcttg gtttttacct acaataaagt
    14161 caactgctaa acctttacca tgatcacctg gatctcccgc acggtatgta ctgaattcat
    14221 taactccata agttgacgct actttttctt tataagctgc aacatgaggt tggagccctg
    14281 cattttcagg atgtgcagct actgcatttg ttgttgaagc tggttgtgct accggtgttg
    14341 ctgttggagc tttttgtgct accggaacgc tcttaacttc agtcgcttgt aacttactgt
    14401 ctgtagctgg tgaagtcgta gtcacaggaa ctgctggagc tgatacatgc tctggtgatg
    14461 caccagtttc tactttagga gtgactactt tagcacttgc cactctaggg gctgccacag
    14521 ttcttaccgg tgctacttta gctactggag ctggtgtttc agcggcaaca gaagctggtg
    14581 atactgttgt taactgactg actgacgtct gagttggttt aacttcctct ttagctgctg
    14641 gaacttctga agtaatcgac ttcacaggag ctggtgatac ctgttcatta gctgctactt
    14701 gactaacagc ttgctcttgt gctaatactt cttttgattt caaagctggc gcagaagaat
    14761 atgtcttcat tggcgaaaca atcgttgttg ctgcttctgg tgtcatacct tccgaaattg
    14821 tattgagaga aactttttgg tctgcaacaa aaacttgatt ggttttcaaa tcgacagtag
    14881 ctgttgtttg accagcagca tttgttgctg gtgtttctat tttcattgaa gtggcagtat
    14941 gactcttctg atcgtaagtt actgtcagtg ttgtctcagg ataaataaga ttgatatctg
    15001 caatgttatt aatttttgct aagacattca tatcaattga cattgcttct gaaataacgc
    15061 ttagtgtatc accatatttc acagtatatg atgatttatt gtcttgcttt accaaatcag
    15121 cctttacctc tgaaacagta cgtgctgtcc acgtcgtatc tgtttcttgt gcttgaacac
    15181 ttgcgactga taatagcgaa gctgccattg tcgatgtcaa tagtaccttt ttattcattt
    15241 tcattgcttc aattcctatt tcctttttat tagtccgaga ctataatatc acactttact
    15301 ggactataaa attattttca gacggtttaa agaaattgta cttagttagt aacaaaactg
    15361 tcatttaaaa caatgaatca atacctcaaa ctatttgtaa agcagttctt gactgttgtc
    15421 aacgcgaagc caaactgaac taccattagc aaagcgtgtc ttagcccaag ttaaaccagc
    15481 tgttccttgg atgggttttt ctacagtctt aagagtttta ggattaaaga taaagtaacc
    15541 acctttttga agaacctggt cagctgtcaa attaccatta gcatcaacct catcaatttc
    15601 tgatgctggt ataccattat cgttccaatc aaacccagtc ggcgttaggg tgttattttt
    15661 gactaaccaa acaccattaa ccttttgtaa ttcatctact cgatacactt ttaatttact
    15721 agattgattc tgtacaggag ctgattgtaa tggcttaatg cttggagctg atgcttgcgt
    15781 ttttccacta aaggtcgcaa cgttagcaat tagtgacgtt ggattgatac gtccatggaa
    15841 accattttga aaattagggt tagctggtaa aaattcaaaa tgaaggtgag gtcccgtcgc
    15901 cgtaccagtt gctcctacgt aaccgatgat atctccttgt ttgacttttt ccccagtcct
    15961 agccaccaca cgtgacatat gagcgtaacc actatgcatt ccatccgcat gttgaatcat
    16021 gacacaattt cctgctaagt ctgtcatcca agaaaagttg gctccagctc ctgcaaattt
    16081 cacagtacca tctgccactg ccctaataat cgttccagtc ggaacagcat aatccacccc
    16141 acaatgtcca ggataaccat tgaaacctgt tgtaattcta ccattatcaa ttggacggac
    16201 ataagtatcc gctaaaactc gggaacccgc agataaaacc ataccaccta aaacaactaa
    16261 ggaacttgcc ttaactaacc atttattcaa gaaaaacctc caaatagaat taatataatg
    16321 aagtgctcaa acacttgcaa ttaacttatt cgaaaattta aatcaaaata tcaaaaaaag
    16381 tactcacctt gatgagtact taggaggttt aataaaaaga aaagttttta ggatatacgt
    16441 agtatacctg agttaactta agaaaaccta aactactatt ttttaatgtc taaaatgtct
    16501 cacgcctgtg aagatcatgg tcaagccatg tttgtttgcg gcgtcaatag attcttgatc
    16561 acgaactgaa ccacctggct ggatgattgc tttgatgcct gcggcagcta tttcttcaat
    16621 gttgtccgca aatgggaaga aggcatctga tgctagaacg gcaccgtcaa ggtggtcctt
    16681 agcctgctcg atagcaatct tgactgagcc gacacggttg gtttgaccta cacccagtcc
    16741 aagcgtcatg tgatcgttag taataataat cccgttagac ttaacatact tgatagcctt
    16801 ccaggcaaac tcaagggcag tcacctcttg ttctgttggc tggcggtctg tcaccacttg
    16861 ccagtcagat ggattttcag ccacaacgtc ttggttttgc accaaaagtc caccaactac
    16921 gccagtgtac tcagcttcca cttcgctggc agcttgggca tcaaacggca actcaaggat
    16981 acgcaagttt ttctttttat ttgtgagaat agctagcgct tcttctgagt atgatggtgc
    17041 gatgatgatt tccaagaaga tagggtgcat cttctcagct gtcgctgcgt caacttcacg
    17101 gttaaggaca acaattccac caaagattga aactggatca gcttcataag cgtaatccca
    17161 agctgtctca atatcatcag cctgtccgat accacatggg ttcatgtgtt tgagggcaac
    17221 aaccgttgga ctgtctttga aatcgcggat aatacggatt gctgcatcag catcacggat
    17281 attattgaag gacaattctt ttccattgag ctgtttagct gaagcgattg agtagtctgt
    17341 tggtaaggct ttttggtaga aatcagcgtc ttgctgtgga ttttctccgt aacgcatagc
    17401 ctgtttaaga tcataagtga tggtcaattt ttcaggctta gcctctccca cttgagctgt
    17461 gaagtactca gcaatcaaag cgtcgtaggc tgccgtatga cggaaggcct tagctgccaa
    17521 gcgttgacga gttttaaatg tcgtctgact agcgtcagcc aattctccca aaacagtggc
    17581 atagtcagct ggatcaacca caacggttac gctagcgtgg tttttagcag ctgagcgaag
    17641 cattgatgga ccgccgatgt cgatgttttc caccgccaaa tcgttggtca cgtctgggcg
    17701 aaggatagtc tccttgaagg gatagaggtt gacaaccacg aggtcaatca actcgatgtt
    17761 gttgtcctta gcagcctgaa ggtggctgtc agcgtcgcga cgagccaaaa tcccaccgtg
    17821 aatgtttgag tggagggtct taacacgacc atccatcatt tctggggagc cagttacatc
    17881 gtcaatagca atggtctcaa caccagcatc gtcaagggca accttagtcc caccagttga
    17941 gataatatcc caacccaagt ttttcaattc ttttgcaaag tcaataattc ctgatttgtc
    18001 agaaactgag attaaagcac gtttagtcat gagttctcct tttcttactt caatctattt
    18061 cataggctat gtattcatga gaaaattcat aaccgagttt ttcagataaa ttcaatgaag
    18121 tccttgtatg agcatcccag ctaggataaa ttcccttatc taaacaagtt agtatcaact
    18181 gagctgcaac tattgttgct aaaccacgcc gacgaaaatc tggatgcgta tctacttcta
    18241 tctcgatccc atttttatag gttgaataag atgaagctcc tgcaatgata ttcccctgat
    18301 aatatataac ataacctata ccttgttttt tatagtactg ataagtagcg taatttgcta
    18361 ctaaatcctg tgaccattcc ttttctaagc aagagttgta aactttctca tcaataacac
    18421 gtaattcaaa accatttggc aactgagtaa caaacttctc taacctactt cgctcaaaca
    18481 aagtatcttt tttcgtcgca taacgcttaa aagaatgggc attctggcca taagttgatt
    18541 caatcaaatc tgaccatcct ttatgctgag gaacaaggat aatatcctcc ccagagcaaa
    18601 cttctagtag aaataaagta ggctgacctg ctagaaagcc aaaagatgat ttccttccca
    18661 attttgccaa agaagatttt ggttggtcta aactatctac aaaaacttct cccataatac
    18721 cttggacaca cgaccagatt atggtctcgt cccaatcacc aaaaatagac tctactatct
    18781 ctaaattttt cactagtttc attagcttat tataacaaat tttactattt tctctttatg
    18841 cccaaactat ccaaaacatc tggatagagt tgatattcgg tttcatggat gcgggtttca
    18901 aagctttcta ggctatcgtc tgctaggcgt ggcacgcgca cttgttggat gacctgaccg
    18961 gtatccacac cagagtccac ccaatggatg gtcacaccag actggtcaac acctgcctcc
    19021 caagcatcct tgataccgtg agcacctgga aattcaggca gataggctgg gtgaatattg
    19081 atgatacgcc cttcataggc tgagagcaag gtttctccga caatcttcat gtagcccgcc
    19141 agacagacca agtcaatctc gtgtttgtcc aacaaatcaa cgacggcttg ctcgtaagcc
    19201 gccttattct caaactcttt gagttcaaaa gcgaagcttg gaatggctaa gttctgagca
    19261 cgctctaaaa cataggcatc acgatgatct gaaaagacaa aactaactgg aaactgctct
    19321 gctatgacct gaaagttgga accattagca gaagcaaaaa cagcgatttt catattcgat
    19381 acaaagggat gattcggctt gccgaaaaat ttctagaaaa ataggaaaac gaacaacgac
    19441 gcttgcgtct aagtcgtttt atcttttttc ccagaaattt aatcccgtgt tcagttaggc
    19501 aaactaacta aacactcctt tcttataaaa tttagtgact tatttaatca ccacactatc
    19561 gtctgctttc ttgatgatac gaccgatttc ataaactggt tcgtccaaga gctctttgac
    19621 gcggttaaca ttttcaggac taacagctag cataagaccg acacccatat tgaagatttc
    19681 aaacatttct tcgtgcttga tgtcaccata tttttcaagc gccttgaaaa tcggaagtac
    19741 tggcacctta tcctcatcga tttccgcagc caaatcatcc gcaaacatac gaggaacatt
    19801 ctcgataaag ccaccacccg tgatgtggcc gataccgttg actagttctt ccttgattaa
    19861 tggcagagct gcttgaacat agatacgagt tggctcaaga aggacatcct tgagttgttt
    19921 accttcaagc tctggaagca gctctttacc agtatagtca gcaaagacac gacgcaccag
    19981 cgaataaccg tttgagtgga taccacttga agcaagtccc aaaagaatat cgccttcctt
    20041 tactttggaa ccatcaagga gctgagattt ttcagccaca ccaacagcaa agccagcaag
    20101 gtcataatca tcttcgccat acatcccagg catttcagcg gtctcacctc caatgagtgc
    20161 tgctcctgct tgaacacaac cttctgcaac gccagaaaca acttcttcaa gtttgactgg
    20221 gttatttttt cctgtcgcaa tataatctaa aaaatataat ggctcagcac ctgctgcaat
    20281 aatatcattg acacacatgg caacacaatc ttggcctatg gtgtcatgct gttgatattt
    20341 aatcgcaagc atgagttttg tgccaacacc gtctgttcct gaaaccaaaa taggctctct
    20401 aactcctgtt ttactcaagt caaacacccc accaaagcca cctagagctc ccatgactcc
    20461 cgcacgttct gtacgagaaa cgtgtttttt tattctttca accacttcat aaccagcttc
    20521 aacatcaaca ccagattttg cataagcatt tttttcagac atgttttctc cttatataat
    20581 tagcagagct tatttcactt tttgaatata aaaacttgtt tcttcttcta aacttctgag
    20641 gtattcttct tcataatcat aaagcggtgt tggataatgt ccatcaaagt aagctacgca
    20701 taaaccacca tttggtgctt ttgtttcgag tccaattgat tcaatcaacc catctagtga
    20761 gagataggtc agactatctg cgccaataat atcacacact tcgtcagcag cgtgatttgc
    20821 tgaaattaat tctcgtcttg tttgaatatc aataccataa aaacaaggat actttaattc
    20881 tggactagct atagcaacat gtacttcact agctcctgct tctcttaata atccgacaat
    20941 ccttctagaa gtcgttcctc ttacaattga gtcatcaatc ataacaacgc gctttccttt
    21001 gacaacacca gataccgctg atagtttcat tcgaacacct tgttccctta attcttgtgt
    21061 cggttgaata aaggttcgct gcgtatactg attttttaca agacccatct catttggtaa
    21121 tccggattct tcagcaaagc ccatagcagc cgataaggac gaatttggga caccaattac
    21181 aatatcagca tcctgtttaa attcttgtgc aagacgcttt cccatatttt ttcgagccgt
    21241 atgaacatta acaccatgta tagttgaatc cggccttgca aaatagacat attccatcga
    21301 acaaattgcc agttgcgttt catcagtata acgatcacat tgaataccac tatcgtctat
    21361 aaggataact tcgccaggtt caacatctct aacccatttt gcgcctacca cctcaaaagc
    21421 acaggtctca ctggaaataa cccaagcacc attttgcatt tgtccaattg acaaaggacg
    21481 aaaggcatta gggtcaagag cagcaattaa tttatcttct gtcatcagta gataggcgaa
    21541 gcctcccttt acagtgctta aagcttcttt taccttcccc ataaaacttg ggttatggct
    21601 tcgacgaatc aagtgcatca aaatttcagt atctgaggag gcattgaaaa ttgcaccttg
    21661 cttttctaat tctttcctta aagaaatagc atttgtcaaa ttaccattat ggcataaagc
    21721 aaattgcccg tcatgaaatt tataaagaaa aggctgaata ttgcgaatat ctgcagaacc
    21781 tgcagtagca taccgaacat gtccaatagc cgcattccca gttaaattat ctaattcaga
    21841 ttgattctta aaaacttcag aaaggagccc aacatttcga taaccataga gtttcccatt
    21901 atcattcgaa acaataccag caccttcttg accgcgatgt tgaaggctat gaagcccaaa
    21961 gtaagtgact tgagctgcct gaggatgccc ccagatacca aagactccac attcttcatt
    22021 tagagatttt acttcgtatg tcattctttt ttcctaattc tttttgtatc tcatcttaag
    22081 tagctttgat gattttctca attttggatc tatcgtcacc aatgccaaca tcaaaataga
    22141 aaatctatca agctattttc aagctaatca ttgagtctta gcctcaacgg ctcccaaata
    22201 ccactacgac agaaagatac agttaagctc gcttcgctct tgtttcctta tctgttttat
    22261 gaaaaaattt gaaaaaatta ttttcctgta aaatatttta ctgcactagc aaacaaggct
    22321 tggtctttgt taccagggat attttggaag aggccgtctt cccagcgttc tgagtgcccc
    22381 atcttaccga tgatttgacc attcttgctg gtaatccctt cgatggcatt gacagagcca
    22441 tttggattgt atttagaatc catagatggt tgtccgtcaa agtccacata ttggctccag
    22501 atttgaccat tgtctcttag ctctgcaaat tcagaagcgc tgacaacaaa tttaccttca
    22561 ccgtgtgaaa ctgggatggc atgaatatcg ccgacctcaa ctcctaccaa ccaaggtgag
    22621 ttggtatttg cgatacgagt ctcaaccatc ttggcaacgt gctggttggc atcgttatag
    22681 aagagggttg gacttgtctc accagcttcc tcgaagtttc cgtatggaag aagacctgat
    22741 ttaacaaggg cttggaaacc attacagata ccgatgataa ggccaccttt ttcgatgaag
    22801 ctgtcaatag ctgcgcgaac cttcttgtta agcaagatat tgacgataaa cttagcagac
    22861 ccatctggtt catccgctgc tgagaatcct ccagcaaaga agatgatatt tgccttagca
    22921 atattagcga ccattgtgtc aactgagtca gcaatagcag cctcattcaa ggttacaaat
    22981 ggtaccaagt tgacactagc tccaacctgt tcaaaggcct tagctgaatc atattctgag
    23041 ttggtaccag ggaagactgg aatgtaaacc actggttttt caattgtttc cttagccttg
    23101 ataacagtat ctgataccac agcaggaact tcttcaagag cgtctgcctg ttcaaactct
    23161 gttgggtaaa cctcttccaa tttgccttcg aagactgata gaaggcttgc cccagcaagg
    23221 tcatttccat tgacagtaag tgtaaaggct gcctgagttt gaccgatttt caccgagtca
    23281 gcaatttcct cagctgatgt aaagacaaag cctccgagtt gagctgtcaa gctactgtca
    23341 agctctgcaa tttcaacaga ggccccgata cggttaccaa aagtcatgag agcaagactt
    23401 tctaggacac caccgtattt aacagctgaa gcagcagtaa tcttatgttg agcttgaata
    23461 gtctcgaact ggctaaagtt agccttgata aggtcaaaat caatatcttc tgaaatagct
    23521 tgacctggaa tgtagtagat attctcacca gccgctttaa actcaggaga gagaaccttg
    23581 cggctgtccg ctgttgtcac accgaaagct accaaggttg gcggtactgt caattcttcg
    23641 aaagtaccag acatagagtc cttaccgccg attgatggca atccaagttg aatctgagcc
    23701 tcaatagaac caagaagagc tgatactggc tgatcaaaac gctctgcctg tttatccata
    23761 cgctcgaaat actcttggta agagaaacgt gcacgagacc agtcagcacc cgttgctacc
    23821 aagcgagctg tcgcttcaat gacagcatag gcagcaccgt ggtaaggtga ccattctgca
    23881 atataaggat tgtaaccttg agccataaca gatgcagttg ttgtgacacc gtgttgaact
    23941 ggcaattttt gaacagagct ttctgttggt gtgatttggt aacggccacc gattgggtgg
    24001 ttaacggttg agcgaccaac agatgagtca aagatagttt ggagaccttt ttgactagcg
    24061 tggttgaggt cagacaagac cttaagcgta tccgcttcaa gtgtctctgc tgatgttgtg
    24121 cgtacctctg gaactgtcaa gtctttgtca acgactttag catcaacaac gacacggaca
    24181 ccgttggtat caaggaaacg gcgttccaag tcaacaattg tttcaccatc ccaagtcatg
    24241 acaaggtttg gttttgcagt aatagtagca accacaacgg catcgatatt ttccttgttg
    24301 caggctgcga tgaaggtatc cacgtcacta ggacgaacaa cgactgacat acgctcttgt
    24361 gattctgaga ttgcaatttc agtaccatta agaccttggt atttaagcgg taccttgtcc
    24421 aaatcgattt caagaccgtc agccaattca ccgatggcaa cacagacacc acctgcaccg
    24481 aagtcatttg atttcttgat taggcgagtg acattaccat cacggaaaag gcgttgaatc
    24541 ttacgttctt cgatggcatt ccctttttgt acctctgcgc cagctgtttc cacagattca
    24601 accgtttgaa ccttagatga acctgtcgca ccgccgacac cgtcacgacc tgttttacca
    24661 ccgagcaaga tgaccacatc gcctgcttct ggtttttcac gaaccacatt ttccttaggt
    24721 gcagcaccaa ccacagctcc aagctccatg cgtttggcta caaagccagg gtgaaagtac
    24781 tcacgcacat aagttgtcgc aagcccaatc tggttaccat atgaagaata gccgtgcgcc
    24841 gcagttttag aaataacctg ttgcggcaat ttaccagcac gtgtttccgc aatcggagtc
    24901 gtaatatcgc ctgcgcctga aatacgcata gcctgataaa cgtatgaacg tcctgacaac
    24961 gggtcacgaa tggcaccacc gatacaagtt gctgcgccac caaatggctc aatttctgtt
    25021 gggtgattgt gagtctcatt cttaaacatg aggagccaag gctcttttac accatcaaca
    25081 tctacttcaa tttcaactga gcaggcattg atttcatctg agacttccat atcatccagt
    25141 cgtccattgg cacgttcata acgaccaaag atagtcgcca tatccataag tgtctgcggc
    25201 ttttcagaac gaccaagttc atcacgcatg gcgatatatt tgtcataagt cgcctgcaat
    25261 tgtttttgga atttagaagc tgaaaagtcg atgtttttca actcagtttc aaaggttgtg
    25321 tgacggcagt ggtctgacca gtaagtatcc aaaactttca actcagtttc agttggcaca
    25381 cgcccgattg atttgaaata atcttggatg aagagaagat catcgacctc catagccaag
    25441 ccctgctctg ccttataggc cgcaaaatcg tcagccttat aagtttcaaa gaaatcaaga
    25501 ctagggattg tcttatcaga cacagagaaa gcctgctctt caagcggcaa ggtaatgtcc
    25561 ttgaaacgtg aatcaacagg attcaaaaga tagttcttaa cggcttcaag ctctgcttct
    25621 gcaatatcct tattgaccaa gtaaagctgg gctgtattga ccttaacctg actgtcactt
    25681 ccaagcaata gcaaagcttc ttgcgaacta gcagcacgtt ggtcaaattg accaggaagc
    25741 gcctcaatgg caaagaaggc aaccttatca agctccgcag tgatttcagt ttccgtcaaa
    25801 agacagtctg tcacctgctc agagaaaata tgcttctcag cacgcgccag caaatcctca
    25861 gccaaattga agacatcata gacctgcaca atacgcaaat ccttcaaaga agtcagttgt
    25921 agattatgcg tcaactcttt cacaagacta gccgatttaa tgtcaaagtc agcctttttc
    25981 tcaacaaaaa tacgtttatt catataagat accaagtccg acaagggagc gactgaaaaa
    26041 taggaggata tctgaaattg agatttcagt aagcccaatc ttttttctga gtttcccgaa
    26101 cggattcaat tccttcctct tctaattaca atgctatcaa cttctcaagt acaacttgat
    26161 agacatcggt caagctacca agatctctac gaaaaacatc cttgtccata tgatttcctt
    26221 cagcatccca tagacggcag ttgtcaggtg agaactcatc agccaagatg attttgccat
    26281 ccttgtcaaa tccgaactcc aacttgaagt caatcaagtt aagcccaatc tgagcaaacc
    26341 aatctttcaa gagttcatta atacggcgag tctctccttt cagataagca atttcttcat
    26401 cattaacaat acccaaaaac ttcatatgct catcattgat aaaaggatca ttcagatcat
    26461 catttttgta gtaaaattcc acaattggag tttcaagaac gcgtccttct tccactccaa
    26521 aacgttttga aaatgagcct gctgtcacat tccgtaatac cacctcaagt ggaataatat
    26581 caactttttt attcaattgt tcgttttttg aaatttgctc aatataatgt gtcaccacac
    26641 cagccatatt taatttctca aaaataagag atgaaatctg attatttagt gctcccttgc
    26701 catcaatagt ctccttacga gctccattca acatagtagc ttggtcttta tagaccgttc
    26761 taatcacatt ttcatctttt gtactataaa tatctttcgc ttttccttta taaataagct
    26821 ggttagtcac ttgattattc gcctttctct tattttaaga gttattctaa cacacaacat
    26881 tatctaatac aacaaaaact ttatttaaaa tcaaaaaaaa gaagaaacgt tcgtattttc
    26941 ttccttttta ttattactta tgttcaatga tataatctaa tagttgttgg acgcttgaca
    27001 tgtgttcgac agcctcatct ggaatctcta gatgaaacac atcttccaaa ttgataataa
    27061 actctgttaa agcgatagag tcaattgcta aatcatcatg taacgatgac tctggtgtga
    27121 tatctaactg tgggtcacca agttgctgcc ttaataattc cagcatcttt tcaaaaactt
    27181 catctcttga catgagttca ctcctattca tctgtaaagg catcaactaa ttgatcaact
    27241 acttgagttt ccaacattgt acgaacttgc ttcaaagtag aataaacagc tttagaatca
    27301 cttgatccat ggcattttac tattggagct tttaaaccaa aaagaaccgc acctcccgcg
    27361 cttgaatagt ccatactatc ttttagctga tacaggctat cttttaatag aagtgctcct
    27421 aatttagctt tcacgccacc tgatttaatt gaagatttta gacttcccat aatattcata
    27481 gctgtacctt ccattgtctt tagaacagca tttccagtaa aaccatcagt gacaacaata
    27541 tctgctacac tagacatcaa atcacgtgct tcaatatttc caataaaatt aatagaaggc
    27601 tctgctgcta acagttcata ggcttcttta tgaagagaat cgccttttgt ttcttccgtt
    27661 ccgttattta agagacctac acgaggaact tcaataccac ggacattttt tgcataaaac
    27721 gaccctaaaa tagcatattg atgaagatga gaagctgtgt tttctgcatt agctcctaaa
    27781 tcaagcatat caaaacccac accatctaac gtaggcatag ttgacattaa gcctggtcga
    27841 tcaacacctt ttatacgtcc tactacaaac aaaccagcag ctaataaagc tcctgtattc
    27901 cctgcagaaa taaaggcctg tgcaacacca tctttgactg ccttagctcc taagaccatc
    27961 gaagactgtt ttttacgacg aacagcttta gcaggctcat catcagaatt aattttttct
    28021 tcagtatgaa taatactaac tcgctccgtt actgttaaat atttctcaat ttttttctga
    28081 tcgccataga gttgtacttc aatatcacta aaatctgaaa tagcttgatt aacaccctct
    28141 acaatagctt tgggagcgta atctccaccc atagcatcta cagcaatttt cttcatgaat
    28201 gaactcctca taaaaactta gtggctatat tatagcatgt ttctttattt ttttcacaag
    28261 aaggcaataa tagctaatat ctaacagata ctttcactct ttccaaagaa aagtatgcta
    28321 aacaacctac taatatcaaa gaacatagta ctcccccttc aaaaccaaaa tccccaccac
    28381 tccaaatagc aaagctagaa ttttcagtaa ttctaaaaag agaaacagga gaataccccc
    28441 cactaacctt attacctaat acacatccca tagtaaaatt ccatgcagta tgaactcctg
    28501 taacaaaaag aatatttttg gtcatattaa aaagaataga aaaaataata ccaagtaaaa
    28561 ataaattaaa aagtgctatt gctgtgatac catagtttct aaagtggata aaggaaaaca
    28621 acatagaatt gcataaaaca cctgcaaaac tacctttaaa tttggtaact gttgtcataa
    28681 catatcctct aaataaaatt tcctctgtca agctttggat aaagtatgca agtaataaaa
    28741 agaaaaaagg tccaaaagaa aaggaattgc tggaagaaat ttccacaaaa cctgataacc
    28801 agtggcaaat ataaataaaa aacatcagta aactaccagt aataaatcct gagatcgttg
    28861 ttaaaacatt attatcttta gtaaatccca aggaagtaaa atcccttttt tgaaaataga
    28921 ttaaaaatag taaaatactt gccaaaatag gaataaatag taaaagcgca atgtataaat
    28981 tgaatttata atcatcaggt agcaatttta ctacaaggcg tctacttaca atgatgccga
    29041 taacttgaat tagaactgca ccaataactg gcacaaacca gtta
//
LOCUS       DATRME010000016        28750 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRME010000016 DATRME010000000
VERSION     DATRME010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28750
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(465..851)
                     /gene="rplQ"
                     /locus_tag="VBC23_000736"
     CDS             complement(465..851)
                     /gene="rplQ"
                     /locus_tag="VBC23_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6842702.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(866..1804)
                     /locus_tag="VBC23_000737"
     CDS             complement(866..1804)
                     /locus_tag="VBC23_000737"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6842703.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1854..2237)
                     /gene="rpsK"
                     /locus_tag="VBC23_000738"
     CDS             complement(1854..2237)
                     /gene="rpsK"
                     /locus_tag="VBC23_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6842704.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2255..2620)
                     /gene="rpsM"
                     /locus_tag="VBC23_000739"
     CDS             complement(2255..2620)
                     /gene="rpsM"
                     /locus_tag="VBC23_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6842705.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2638..2754)
                     /gene="rpmJ"
                     /locus_tag="VBC23_000740"
     CDS             complement(2638..2754)
                     /gene="rpmJ"
                     /locus_tag="VBC23_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6842706.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2780..2998)
                     /gene="infA"
                     /locus_tag="VBC23_000741"
     CDS             complement(2780..2998)
                     /gene="infA"
                     /locus_tag="VBC23_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6842707.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3114..3752)
                     /locus_tag="VBC23_000742"
     CDS             complement(3114..3752)
                     /locus_tag="VBC23_000742"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6842708.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3847..5151)
                     /gene="secY"
                     /locus_tag="VBC23_000743"
     CDS             complement(3847..5151)
                     /gene="secY"
                     /locus_tag="VBC23_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6842709.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5172..5612)
                     /gene="rplO"
                     /locus_tag="VBC23_000744"
     CDS             complement(5172..5612)
                     /gene="rplO"
                     /locus_tag="VBC23_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6842710.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5737..5916)
                     /gene="rpmD"
                     /locus_tag="VBC23_000745"
     CDS             complement(5737..5916)
                     /gene="rpmD"
                     /locus_tag="VBC23_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6842711.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5931..6425)
                     /gene="rpsE"
                     /locus_tag="VBC23_000746"
     CDS             complement(5931..6425)
                     /gene="rpsE"
                     /locus_tag="VBC23_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6842712.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6444..6800)
                     /gene="rplR"
                     /locus_tag="VBC23_000747"
     CDS             complement(6444..6800)
                     /gene="rplR"
                     /locus_tag="VBC23_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6842713.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6901..7437)
                     /gene="rplF"
                     /locus_tag="VBC23_000748"
     CDS             complement(6901..7437)
                     /gene="rplF"
                     /locus_tag="VBC23_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6842714.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7547..7945)
                     /gene="rpsH"
                     /locus_tag="VBC23_000749"
     CDS             complement(7547..7945)
                     /gene="rpsH"
                     /locus_tag="VBC23_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6842715.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(8100..8285)
                     /locus_tag="VBC23_000750"
     CDS             complement(8100..8285)
                     /locus_tag="VBC23_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6842716.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8303..8845)
                     /gene="rplE"
                     /locus_tag="VBC23_000751"
     CDS             complement(8303..8845)
                     /gene="rplE"
                     /locus_tag="VBC23_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6842717.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8869..9174)
                     /gene="rplX"
                     /locus_tag="VBC23_000752"
     CDS             complement(8869..9174)
                     /gene="rplX"
                     /locus_tag="VBC23_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6842718.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9254..9622)
                     /gene="rplN"
                     /locus_tag="VBC23_000753"
     CDS             complement(9254..9622)
                     /gene="rplN"
                     /locus_tag="VBC23_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6842719.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9647..9907)
                     /gene="rpsQ"
                     /locus_tag="VBC23_000754"
     CDS             complement(9647..9907)
                     /gene="rpsQ"
                     /locus_tag="VBC23_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6842720.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9933..10139)
                     /locus_tag="VBC23_000755"
     CDS             complement(9933..10139)
                     /locus_tag="VBC23_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6842721.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10149..10562)
                     /gene="rplP"
                     /locus_tag="VBC23_000756"
     CDS             complement(10149..10562)
                     /gene="rplP"
                     /locus_tag="VBC23_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6842722.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10566..11219)
                     /gene="rpsC"
                     /locus_tag="VBC23_000757"
     CDS             complement(10566..11219)
                     /gene="rpsC"
                     /locus_tag="VBC23_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6842723.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11232..11576)
                     /gene="rplV"
                     /locus_tag="VBC23_000758"
     CDS             complement(11232..11576)
                     /gene="rplV"
                     /locus_tag="VBC23_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6842724.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11592..11870)
                     /gene="rpsS"
                     /locus_tag="VBC23_000759"
     CDS             complement(11592..11870)
                     /gene="rpsS"
                     /locus_tag="VBC23_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6842725.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11969..12802)
                     /gene="rplB"
                     /locus_tag="VBC23_000760"
     CDS             complement(11969..12802)
                     /gene="rplB"
                     /locus_tag="VBC23_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6842726.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12820..13116)
                     /locus_tag="VBC23_000761"
     CDS             complement(12820..13116)
                     /locus_tag="VBC23_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6842727.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13116..13739)
                     /gene="rplD"
                     /locus_tag="VBC23_000762"
     CDS             complement(13116..13739)
                     /gene="rplD"
                     /locus_tag="VBC23_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6842728.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13763..14389)
                     /gene="rplC"
                     /locus_tag="VBC23_000763"
     CDS             complement(13763..14389)
                     /gene="rplC"
                     /locus_tag="VBC23_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6842729.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14462..14770)
                     /gene="rpsJ"
                     /locus_tag="VBC23_000764"
     CDS             complement(14462..14770)
                     /gene="rpsJ"
                     /locus_tag="VBC23_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6842730.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14977..16257)
                     /locus_tag="VBC23_000765"
     CDS             complement(14977..16257)
                     /locus_tag="VBC23_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6842731.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16362..17852)
                     /locus_tag="VBC23_000766"
     CDS             complement(16362..17852)
                     /locus_tag="VBC23_000766"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6842732.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17972..18988)
                     /gene="adhP"
                     /locus_tag="VBC23_000767"
     CDS             complement(17972..18988)
                     /gene="adhP"
                     /locus_tag="VBC23_000767"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6842733.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19166..21808)
                     /gene="adhE"
                     /locus_tag="VBC23_000768"
                     /gene_synonym="adhC"
     CDS             complement(19166..21808)
                     /gene="adhE"
                     /locus_tag="VBC23_000768"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6842734.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(22077..23855)
                     /locus_tag="VBC23_000769"
     CDS             complement(22077..23855)
                     /locus_tag="VBC23_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6842735.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23852..24232)
                     /locus_tag="VBC23_000770"
     CDS             complement(23852..24232)
                     /locus_tag="VBC23_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842736.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24239..24676)
                     /locus_tag="VBC23_000771"
     CDS             complement(24239..24676)
                     /locus_tag="VBC23_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6842737.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24828..25826)
                     /gene="ruvB"
                     /locus_tag="VBC23_000772"
     CDS             complement(24828..25826)
                     /gene="ruvB"
                     /locus_tag="VBC23_000772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6842738.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(26123..27034)
                     /locus_tag="VBC23_000773"
     CDS             complement(26123..27034)
                     /locus_tag="VBC23_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842739.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLND
                     LIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFV
                     GLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQS
                     IMFSKMIDDKHLIKKLELEWQEDITGH"
     gene            complement(27187..28485)
                     /gene="purB"
                     /locus_tag="VBC23_000774"
     CDS             complement(27187..28485)
                     /gene="purB"
                     /locus_tag="VBC23_000774"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6842740.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            complement(28503..>28750)
                     /locus_tag="VBC23_000775"
     CDS             complement(28503..>28750)
                     /locus_tag="VBC23_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842741.1"
                     /translation="IHKSVEIPWTDMVEQLNLIINTQLSTADKAIYSELLEISEAEDE
                     RKLKNFVNKMSSFFKNDYVQNTITGVLGNVISSILMK"
BASE COUNT         8654 a         5971 c         4855 g         9270 t
ORIGIN      
        1 tgcctctctt gagacaactt ctatattcta gcaaacttat tctgctttgt caaccacttt
       61 ttttaagttg ttaactacgc gctactagaa actgctctct cgagacaact tattcattat
      121 actaaatatt tctacttcct gtcaatacta tttttgtatt ttataaattt agtatagaca
      181 taactattcc tctatattca attaagagaa attatataac cactattgag aaatgtagtc
      241 tagctattaa actctttgct catcgaaaat atccaataaa tataatacat aaaacgcctg
      301 cttacgaaat ataaacaaaa ttgtttgcat ttcgtaaaca agcgttacct atttaacaat
      361 atatgatgag tgttcccgct gagaataatt ctcagcggta gaccagagct agactaagaa
      421 tcgattgatt ccatcatcat aacactcaac aaaattgata aaaattatac taattcaata
      481 attgccattg gggcagcatc gccacggcgt ggttctgttt taagaatacg agtgtatcct
      541 ccgttacgtt cagcataacg aggtgcgatg tcgtcaaaaa gtttttgaag agctgttgtt
      601 gatgtatatt tatcagaagc ttcatcatag ttttctgatg caatttcatt acgtacataa
      661 gcagcagctt gacgacgagc atgtaaatca ccacgtttac ctagagtaat cattttttca
      721 actgttttac ggatttcttt agcacgtgct tcagtagtta caattgattc gttgataaga
      781 agatcggttg tcaaatcacg aagcattgcc ttacgttgtg agctagtgcg tcctagttta
      841 cggtaagcca ttatgtcctc ctattttatt tatcgttttt taatccaaga cctagatcgg
      901 caagtttgat tttaacttct tcaagactct tacgtcctaa gtttcggact ttcatcattt
      961 caggctcagt tttttctgtt aaatcaaata ctgtattaat tccagcacgt tttaaacagt
     1021 tatatgagcg cactgacaaa tcaagttcct caattgtgcg gtcaagcact ttctcatcgt
     1081 tcactttctc tgtttccttc attacttcag ttgctttagc aacctctgtt aaatcagtaa
     1141 acaagtttaa gtgttcaatt aagacgcgag ctgaaagacc aagagcatcc tcaggaatga
     1201 ttgtgccatt tgtcatgatt tcaatagtta atttatcaaa gccatcatta ctacctacac
     1261 gagcaggttc aacttgataa ttaacttttt tcactggtgt atagattgaa tctacagcca
     1321 atgttcctac tggtgcatca tcttttttat ttccttctgc tggaacataa cctcggtttt
     1381 tagcaacggt cattgttgct ttcaaactgt gaccttcagc aattgtgaaa agataatggt
     1441 cagggtttac aatttcgata tcactatctg ttaaaatatc tccggcagta atttctgcag
     1501 gaccttcaac gtctagttcg ataatctttt cgtcttcgac gtaagatttc acagcaagtc
     1561 cttttacatt aaggatgatt tgcatcacat cttcacgtac acctgggata gtatcaaatt
     1621 cgtgtaatac tccatcaatt ttgattgatg tgactgctgc acctggaagt gaagacagaa
     1681 gcacacgacg aagagagttt cctagagttg taccgtagcc acgttctagt ggttcgatga
     1741 caaatctgcc gtaatcttta ttttcatcaa tttttgttat tattggtttt tcaaactcaa
     1801 tcatttagtt actccctctt aaacgaaaag ttgtgtacta taaaatgcta tgattataca
     1861 cgacgacgtt ttggaggacg agcaccatta tgtggtacag gagtcacatc acgaattgca
     1921 gttacttcaa gaccagctgc agcaagggca cggatagcag attcacgacc tgaaccagga
     1981 ccttttacag taacttcaac agttttaaga ccgtgttctt gtgcagattt agcagcagct
     2041 tcagcagcca tttgagcagc aaatggagta gatttacgag aacctttaaa tccaagagca
     2101 ccagctgatg accatgcaag agcgttacca tgcacatctg taatcataac aatagtgtta
     2161 ttgaatgtag cgtgaatatg agcaacacca gattcgatgt tctttttcac acgacgtttg
     2221 cgtgttggtt tagccaattg ttttacctcc tattttattt tttcttacct gcaatcgcaa
     2281 cagctttacc tttacgagtg cgagcgttat ttttagtatt ttgtccacgg acaggaagtc
     2341 cacgacggtg acggattcca cggtatgaac cgatttccat caaacgttta atattcaagt
     2401 ttacctcacg acgtaggtca ccttcaactt tgattgcatc aacttcacga cggatagcgt
     2461 cctcttgatc tggtgtcaaa tctttaacgc ggatatcttc agaaatacct gcagctgcta
     2521 agattttttt agatgtagaa agaccaattc cgtatacata agtaagtgaa attactacac
     2581 gtttgtcatt tggaatatca actccagcaa tacgagccat tttatctcct ttctatatta
     2641 tccttgacgt tgtttgtgtt ttggatttgt cggacaaatt accataacac gaccgttacg
     2701 acgaataact ttacagtatt cgcaaattgg tttaaccgat ggtcttacct tcatgtttta
     2761 atccctccaa ttatttcgac tatttaaagc ggtatgtgat gcgtccacgt gttaaatcgt
     2821 atgggctcat ttcaacagta acacgatcac ctaccaaaat acggatgtag tttttacgga
     2881 tttttcctga tacagttgcc aaaatttggt gtccattttc caactcaaca gtaaacattg
     2941 cgttaggcat agtttcaaca actttacctt caatttcaat cacatcttct tttgccacgc
     3001 aaaagtaccc cctaaatttc gatttgatgt cctctataaa gaggtaacaa ttataagtca
     3061 gactaactta gtatatcatt actaatctac ttatgcaagt agattagcat aatttatttg
     3121 agttctagca acgctttttc aacatctgca aaaacttctg ttatttcttg attaccttca
     3181 atatctgtaa caagaccaag cttacgatag tgttcaagaa taggttctcc ttgagcaata
     3241 ttaacgtcca agcgacgttt gacagtttca ggcttatcat cttcacgttg atagtaatct
     3301 tcttctttat aatctactgg tgggttgaac actttgtgga aagtttcacc agttttacga
     3361 ttgataatac ggccactcaa acgctctata aggcatgttg gatccacttt aatattaata
     3421 acaccatcta agcgtagtcc tagttcttca agcgtagcat ctaaggcgtg tgcttgctca
     3481 atagtacgtg gatatccatc aagtaaaaaa cctttttctg cgatatcatc ctcagctaag
     3541 cgctctttta caatcccgtt tgttacttca tcaggaacca attcaccttt atcaatataa
     3601 cttttagcta aacgtcccat ttgggtttga ttagccattg cggcgcggaa catatcccct
     3661 gttgagatgt gagcaacacc aaattcttca acaatcttag ctgcttgagt acctttacca
     3721 gcaccaggca aacccataat taaaagattc attatagtct ccttattttg tttttaaata
     3781 aatccaacac agcatgtcgg acttattaaa aaacaaaata gaaggttgac agagtcaacc
     3841 tatattctat tctgttgtat tcatgaatcc aacatagcgt ctcttcaaaa gatagccctc
     3901 aagttgtttc ataccttcaa tacctgttgc aatcaaaatg agtaagcttg taccacctaa
     3961 cgcaatactt gatgacaaat gcaacgcttg ttgagcaata attggtaaca aggaaataaa
     4021 tgctagaaat actgaaccaa ttgttgctaa ttttttcaat agcgatgaca tatactcttc
     4081 tgtttctcgt cctggtcgaa tacttggaat gtaggacgaa tttttttgaa gattctctgc
     4141 tgtcttttca gggtttactt gtacaaaagt atagaagaaa gagaacagaa taatcaagat
     4201 agcatagata atcataccga ctggggtttg ataattaagt aattcttgga gtttagtaag
     4261 ccaaggaatt tctttaccat tttgaaagaa aggaataatc gtacttggaa ttgttgtaat
     4321 cgagctagca aagataacgg gaataacacc agctggattt acttttagag gaagatatga
     4381 acttgtagga gctccttgaa caagttttgt atattggatt ggaattttgt attcagcttg
     4441 ttgaataaat gttgtaaaaa atacaattgc taatactgct acaataagaa tcccgacaaa
     4501 gatgtaagaa tttgtaattg ctgaagagcg tacatttaca aagaaatctt cgtagatagt
     4561 tgtaattgca cttggaatgg aagaaatgat ccccgcaaag ataatcatag acactccatt
     4621 tccgaatccc ttatcagtaa tttgttcacc tagccaagta accaccatac tacctgttgt
     4681 aaggattgca ccaataagca aatatgtttg tacattcgga gttttaacta aagcaacact
     4741 tgatagagtg ttaaaaccag cagtaatacc aattgattgt acgaatgcaa ggaacaaaga
     4801 aatataacgt gtagcttggt taagcttacg acgacctacc tctccttgtt ttccccattc
     4861 aacaaacttc ggaagaatat ccatttgaag tagctgaaca acaatagacg cagtaatgta
     4921 tgggctaact cccatagaga ataccgaaaa gtttctcatt gcattaccac taactaagtt
     4981 aagcatattt agaaatggaa gttcacccat ttgttctaaa ctcttaacat tgatgcccgg
     5041 tactgtgata tgcgttccga tacgaaatac aagaaggata aagatagtaa ataaaatttt
     5101 atttcttacc atctttactt tgagtgcatc tctaagtaat tttaaaaaca taatgagtta
     5161 cctctcatta attagatgac ttcgattgaa ccacctttag cagtgatagc tgcttcagca
     5221 gatttagaaa atttagctgc tttaacagac aatttcttgg ttaattcacc attaccaaga
     5281 attttaacgc ctgatttttc agcacgcacg attccagctt ctttaagaac aactggagtt
     5341 acttcagtac catcttcaaa gacatttaat tggtcaaggt tcacaagagc atattctttt
     5401 gcattgatgt ttgagaaccc acgttttggc atacgacgga acaatggagt ttgtccacct
     5461 tcaaaaccaa ggcgtactcc accaccacta cgcgcttttt gtcctttttg accacgtcca
     5521 gaagttttac cattacctga agatgtaccg cgacccacac gattgcggac tttacgtgat
     5581 ccttctgcag gttttaattc atgaagtttc attatttttt ctccttattt gtaaaatgct
     5641 aacgccttta cgaggggaaa ggtactcccc gcaaagactc gcctatacga tttgtttaaa
     5701 tcgcaccaat aacaatcagt gcgatttagc ttttaattaa gcttcttcaa cagttactaa
     5761 gtgagagata gcgttaacca taccacggat ggcagcatta tcttccttaa caactgaaga
     5821 gtttaattta ccaagtccaa gggcaacaac tgttttacgt tgttctggtt tgcgaccgat
     5881 tggagactta gtcaaagtaa ttttaatttg agccattata atcccctttc ttatgctaag
     5941 tcagaaacag aaatgccacg taaagcagca acttcttctg cacgttttaa ttgtttcaag
     6001 ccctcaacag ttgcgcgaac aatgttgatt ggagtgtttg aaccaagtga tttagatgta
     6061 atatctgcaa cacctgctaa ttcgatgacg gcacgaacag caccaccagc ggcaacccca
     6121 gcaccctcaa cagctggttt caacaatact ttcgctccac caaattctga acgaacttcg
     6181 tgaggaattg ttgtaccgac cataggaact tcaaccatgt ttttcttagc agcttcaaca
     6241 gctttgcgaa tagcttctgg tacttcttga gctttacctg taccaaaacc aacacgacca
     6301 ttacggtcac caacaactac aagagctgca aaacgtagac gacgtccacc tttaacaact
     6361 tttgtaacgc ggttaatcgc aacaacgcgc tcttcaagtt ctactgcatt atctttaaat
     6421 gccattatta agtgtcctcc ctattagaat ttcaatccgt tttcacgagc tgagtcagcc
     6481 aaagctttta cacgtccgtg atagagatat ccaccgcggt caaacaccac ttcagaaata
     6541 cctttagcta ctgcgcgttc agcaacaagt ttaccgacaa caacggcttg ttctgttttt
     6601 gttccgttag aaacttcttt atcaagagtt gatgcacttg cgagcgttac acccgctacg
     6661 tcatcaatta cttgagcgta gatgcctgta ttagaacgga aaatgttcaa acgtgggcga
     6721 tcagcagttc cagagagttt accgcgaacg cgacggtggc gtttttggcg gattttattt
     6781 ttatctggtt tcgaaatcac aattttcacc tcttaaatat attgttatgt caaaatgaca
     6841 atattaaaat gaaaagatag agaatttgca gaaaagattc tacaaaatct atcaatccta
     6901 ttatttacct gttttacctt ctttacggcg tacgaattca ccaacgtaac gaataccttt
     6961 acctttatat ggttctggtg aacgtaagct acgaacgtaa gcagcagttt gtccaacgct
     7021 ttctttgttg ataccgatta cgttgattgt tgttggagtt ggcacttcaa aagtaacacc
     7081 ttctggagct tcaacttcgt cttggtgaga cttaccaaca gaaagaacaa gttttgatcc
     7141 ttgaagttgt gcacggtaac caacaccgcg catttcaaga gcttttttga aaccttcaga
     7201 aacaccaacg accatgttgt tcaagttcgc acgagttgta ccatgaattg ttttcatttc
     7261 ttttgagtcg ttaggacgag taactgttac ttcagtccct tcaacagtga ttccaatatt
     7321 tttattaaac tcacgagtga gttcgccttt aggtcctttt acagtaacaa cattatcctt
     7381 gttaatgatt tcaacacctg caggcaatgt aattacttta ttaccaatac gtgacatgtt
     7441 tattttctcc tgttaaattg tcaagctgct tgagcaacta gttttcacgg ggactcatga
     7501 gaccacagga ttatactgtg actcaatgag cattttaggt aaaattttac caaatatatg
     7561 caagtacttc tccaccaaca tttttttggc gagcttcttt gtcagtcaaa agaccttcag
     7621 aagttgatac aattgcaata ccaagtccgt taagaacttt aggcatatct tcatgtttag
     7681 tataaacacg aagacctggt tttgaaatac gtttcaagtt agtgataaca cgttcaccat
     7741 tttgtccata tttaaggaac acacggatga tgccttgttt gtcatcttca ataacttcaa
     7801 cgttttttac aaaaccttca cgtttaagga tatcagcaat ccctttttta atgtttgatg
     7861 caggtacttc taacacttca tgttttgctt ggttagcatt acgaatacga gttaaaaagt
     7921 ctgcaattgg gtcagtcata accatttttt aattctcctc ttattagcag tttgaattta
     7981 atcacttgct aattaatgag tccttacgga cgttgtaaga ctaggtctta gtatatttgc
     8041 gtttaaaaat cagtgccatt atcaatgaca ccttttaaac acaaagcatc attaatttat
     8101 taccaagatg ctttagttac gcctggtact tgacctttat aagccaagtc acggaagcaa
     8161 acacggcaaa gttggaattt gcggtaaact gagtgtggac gtccgcattt ttcacaacga
     8221 gtgtaagctt gcgtagagaa cttcgctggg cgtttgttct tagcaatcat agatttttta
     8281 gccaatttat ttacctccta gattactttg caaaaggcat tccaaggcct tttagcaatt
     8341 cacgtgactc ttcatcagtg ttagcagtag taacgataac gatatcaaga ccacgtactt
     8401 tatcaacgtc atcaaagttg atttctggga agatcaattg ttctttaact ccaagagtgt
     8461 agtttccacg tccatcaaat gatttcgttg gaacaccatg gaagtcacgt acacgtggaa
     8521 gtgaaacaga aactaattta tccaagaatt cgtacatacg ttcaccacgg agggtaactt
     8581 ttgcaccgat cgctacacct tcacgaagac ggaagccagc gattgatttc ttagctttag
     8641 taataagtgg tttttgacct gaaataagtg ctaactcagc agcagctttc tcaagatttt
     8701 tagcgtttga tacagcatca ccaacaccca tgttaagaac gattttctca acttttggca
     8761 cagccataac agatgaatag ttaaattttt ctgtcaacgc aggaacaact tcattagtat
     8821 atttttcttt taagcgattt gccattatgc ttctcctttc cttcgtgatt aatcaagcac
     8881 ttcgcctgat tttttgttgt aacgaacttt tttgccgtca acaactttgt aaccaacacg
     8941 tcctgctaca ccatttttat caagaacttg aacgtttgac acatggattg gagcttcttt
     9001 ttcaacgata gcgccttgag ggttttcgtt atttggtttt tgatgtttct tgatgagggc
     9061 tacgccttca acaacaactt tgtttacttt tggaagagct ttaagaacta cagcttcagt
     9121 gcctttgtcc ttaccagcaa taacgcgaac tttgtcgcct ttttttacaa acatttgagt
     9181 ttttctccta tttttcttac gcctcgaaag gcaccctagg aaatcctagg ggactacgtt
     9241 tgttaatctg agattaaagt acttctggtg ccaatgaaac gatcttcatg tagccacctt
     9301 cacgcaattc acgtgcaact gggccaaaga tacgagttcc gcgaggagtt ttatcgtcac
     9361 ggatgattac agcagcattg tcgtcaaatt tgatgtatga accgtctggg cggcgtgcac
     9421 cagtttttgt acgaacgata acagctttaa ccacatcacc ttttttaact gctccaccag
     9481 gagtagcttg ttttacagaa gcaacgatta cgtcaccgat gttagcgaat ttacgtcctg
     9541 aaccaccaag tactttgata gtcaagatct cacgagcacc gctattatca gcaactttca
     9601 agcgagtttc ttgttgaatc atttcaattt tctcctttta gtttgattag ataataacag
     9661 ctttttccac tacttccaca agacggaaac gttttgtagc tgaaagtggg cgagtttcca
     9721 tgatacgaac gatatcgcct tctttagcaa cgttgttttc atcatgtgct ttatattttt
     9781 tagaatagtt gatacgttta ccatagactg ggtggttacg tttagtttca actacaactg
     9841 tgattgtttt gtccatcttg tcagacacta cgcgtccgta aagagtttta cgttgattac
     9901 gttccattta gaatttctcc tttcccaatc tattatttca tttctgattg cacagtctta
     9961 acacgtgcaa tttgtttctt aacttcgtca aggcgagcag tcttttcaag ttgacctgct
    10021 gcagcttgga aacgaagatc aaacaattct tttttgagtt cgttttcttt cttagcaagt
    10081 tcttcttgag acaatccacg aagctcttta acaaaatctt taatttcttg aagtttcatg
    10141 tcttctcctt attctgcttc acgttttacg aatttgcatt taactggtaa tttgtggcta
    10201 gcaagacgta atgcttcacg tgcaacttct tcagaaacac cagcaatttc aaacatcact
    10261 ttaccacgtt taactggtgc tacccaacct tcaggtgtcc ctttaccaga acccatacgt
    10321 acaccgatag cttttgcagt gtatgatttg tgtgggaaga ttttaatcca aactttacca
    10381 ccacgtttca tgtaacgagt catcgcaata cgggcagctt cgatttgacg gtttgtaatc
    10441 cagtggctag ttgtagcttg aagtccgtat tcaccgaatg aaacttcttt accaccttta
    10501 gcctcaccgc gcatttttcc acggaattcg cgacggtgtt taacacgttt aggtactaac
    10561 atttgttatt tgcctccttt agtgttttta cgagctggaa gaacttctcc acggtaaatc
    10621 caaactttaa cgccaagttt accataagtt gtgtcagctt cttcccaagc atagtcgata
    10681 tcagcacgaa gcgtgtgaag tggaacagtt ccttctgaat aaccttcagc acgagcgata
    10741 tcagcaccgt tcaaacgacc agaaacttga gttttaatac ctttagctcc tgcacgcatt
    10801 gtacgttgga ttgcttgttt ttgagcacga cggaaagcaa cacgttgctc taattgacga
    10861 gcaatatttt caccaacaag gtgagcatct aaatcaggtt gtttgatttc aatgatgttg
    10921 atgtgtactt gttttccagt taatttgtta agttgaccac gaagagcgtc aacgtttgct
    10981 ccacctttac cgataaccat acctggttta gcagtgtgaa gtgaaacaat aactttattt
    11041 actgcacgtt caatttcaat agttgaaact gatgcgtcag ctaattcttt attaatgaat
    11101 ttacggattg caagatcttc atgaaggtaa tccgcgtatt ctttttcagc ataccatttc
    11161 gcatcccagt cacggatgat tccgacacgc ataccaattg gatgtacttt ttgacccacg
    11221 attttacctc cttatttttc tgatacaact acagttacgt gagttgtacg tttgttgatt
    11281 ggtgaagctg aacctttcgc acgtggacgg aaacgtttca ttgttggtcc ttcgttagcg
    11341 aaagtttcag atactaccaa gtttgctttt tccaaaccaa agttgttttc tgcatttgca
    11401 attgctgagt taagagtttt ctcaataaca cgagctgctt tgtttggagt gaattttaag
    11461 attgcgattg cgtcagcaac attcttacca cggataagat caagtactaa acgtgtttta
    11521 cgaggtgaaa cacgcactgt acgagccatt gctttagctg aagtaatttc tgccattgtg
    11581 tcctcctcct attaacgacg tgttttcttg tcgtcagctg cgtgaccttt gtaagtacgt
    11641 gttggtgcaa attcaccaag tttgtgacct accatgtcct cttggatata aacaggaaca
    11701 tgtttacgtc catcataaac tgcgattgtg tatccgatga aacttgggaa aatcgttgaa
    11761 cgacgtgacc aagttttgat tacttttttc ttttcgtcat ttgcttgagc ttcaactttt
    11821 ttcattaaat gctcgtcgac gaaaggtcct tttttaagac tacgtcccat tttgtagttt
    11881 tctcctttaa ataatgtacc acaacggcct gtacactata gtgtactacc gagctggcgg
    11941 atttgtataa gattatgcta ttaacctatt atttttggtt acgacgacga acaataagtt
    12001 tgtctgattt agctttctta ttacgagttt taagtccaag cgcaggttta ccccatggag
    12061 tagatggtgc tttacgacca actggtgctt taccttcacc accaccgtgt gggtgatcgt
    12121 tagggttcat tacagaacca cgaactgtag ggcgaacacc tttccaacga ttacgtcctg
    12181 ctttaccaat gttaacaagt gattgctgtt cattaccaac agtaccaatt gttgcactgc
    12241 aagtaccaag gatcatacgt acttcacctg attgaaggcg aacaagaaca tatttacctt
    12301 cttgaccaag tacttgagca gaagcaccag cggcacgaat caattcagca cctttacctg
    12361 gttgcaattc aatattatgg ataactgtac cgactggaat gttagcaagc ggaagtgcgt
    12421 taccaacttt gatgtctgct tctggaccag aaataatgcg ttgacctact tcaaggcctt
    12481 taggcgcaag gatgtaagct ttaacaccat cagtatagtg tacaagagca atatttgcag
    12541 tacgatttgg atcgtattca attgttttaa caactgcttc aacaccatct ttattacgtt
    12601 tgaagtcaat caaacggtaa tgacgtttgt gtccaccacc ttggtgacga acagtaatac
    12661 gtccgttgtt gttacgtcct gctttattct ttagtgaaac aagcaatgat ttctcaggag
    12721 tgtttgtagt gatttctgca aaatccaaag aagtcatatt acgacggcca tttgtcgttg
    12781 gtttataaac tttaataccc acgttatttc ctccttagat tattcagctt cagcagcaaa
    12841 caactcgatt gcttttgaat cagctgtaag agtgataata gcttttttag tttttgaagt
    12901 aaaacctgtg taacgaccaa cacgttttgc tttgggttta actgtcacag tgtttacact
    12961 tgcaactttt actccgtcaa atgcagcttc aacagcttgt ttgatcaaaa gtttgtgtgc
    13021 acgtgtgtca acttcaaatg tatatttccc tgcttcaaga gcaaccattg atttctcagt
    13081 gataacaggt tttttgatta cgtcatacaa attcattatg caagaacccc ctcgattgta
    13141 gagattgctt ctttagtaac aagaagtttg tctgcgttaa cgatatcaag aacacttgca
    13201 gtagttgcag tagcaactgt aacgtttgga aggttacgcg ctgaaagtgc agcaaattcg
    13261 ttaccttctt caaggattac aagtactttt gaatcaatgc taagtgctga aagaactgac
    13321 gcaaattcag cagtttttgg agctgcaaat gaaaggtttt ctacagctac aaatttatct
    13381 tcagcaactt ttgctgaata aactgatttc aacgcaaggc gacgaacttt ttgtggaagt
    13441 ttgtagccgt atgaacgtgg agttggtcca aagacaacac caccaccacg ccattgtggt
    13501 gagcggatag aaccttgacg agcgcgtcca gttccttttt gacgccatgg tttacgtcca
    13561 ccaccagata ctgctgaacg gtttttaacc gcgtgagtac cttggcgaag gctagcgcgt
    13621 tggctgatta caacatcaaa aacaactgat tcgtttggtt cgataccgaa gatagcttcg
    13681 ttaagctcaa ctgagctaac ttctttacca gtttggtcaa atagtttaac gtttgccatt
    13741 ttaactgttt tctcctttct tattatttag cagctttaac tgctgatttg atagtgataa
    13801 gagatttctt agcacctggt acgttacctt tgatgaggac aacgtttttc tctgggataa
    13861 cttgtacaat ttcaaggttt tgaactgtta cacggttacc acccatacgt cctgccaagc
    13921 gtttgttttt gaaaacgcgg ttaggcgcaa caggtcccat agaacctgga cggcgatggt
    13981 aacgagaacc gtgagccata ggaccacgtg attgaccatg gcgtttgata acaccttgga
    14041 aacctttacc ttttgatgtc cctgtgacgt caacaacatc accagcttca aattgttcta
    14101 cagataattc tgcaccaact tctaagcctt caatgttttt gaattcacga atgaagcgct
    14161 taggagctgt gtttgctttt gcaacatggc ctttggcagg tttgttactc aatacttcac
    14221 gtttgtcatc aaaaccaacc tgaactgctt cgtaaccgtc tgtttcaaca gttttaactt
    14281 gaagcacaac gtttggagtt gcttcaatga cagtaacagg gatgaattcg cctgattcag
    14341 tgaagatttg agtcattccc acttttttcc ctaagattcc ttttgtcatg agaaaatatt
    14401 tccttttctt attttatttc aaaaagtttt ttacgagcgt tttttgtgct cagaaatcaa
    14461 attaaagttt gatttctacg ttgacaccac ttggaagatc aagtttcatc aaagcatcaa
    14521 cagttttttg tgttgggttg atgatgtcta ccaaacgttt gtgtgtacgc atttcaaact
    14581 gttcgcgaga atctttatat ttgtgagtcg cacgaataat tgtgtaaaga ctgcgttcag
    14641 ttggaagcgg aactggtcca gcaactgtag cacctgtacg tgttgcagtt tcaacgattt
    14701 tttctgccgc tgtatcaagt gtacggtgtt cgtacgcttt caaacggata cggatttttt
    14761 tgtttgccat cttttctcct ctcgtctatt tacgataata ggctagctcc tcaagaaaac
    14821 caacacgagt tgcgtggcaa tgcaacctag cgtgtcgcaa cctcttgcat catagctaaa
    14881 gctgttcata cacagcacca gtatatgata tcaaaaaaaa acagcacacg caagcattat
    14941 tttatttttt ttcaaaaaaa atgcaaccat ttctggttac attttttgaa tatatcgatg
    15001 atagcgaact ttcaaaaaca gccacctaaa gatattatct gctatcgtag ccatccaaac
    15061 cccaagcaaa cctaattcaa aaactattcc taataaataa ccaagaacta ctctgattaa
    15121 ccacatgcct atagtcgttg tgtaaaatgg gagtttagca tttcctaatc cttgccaagc
    15181 tgctgtataa accaaggtcc caatagtagc aggtactcct acgaaagata gcaaaataac
    15241 aatcaaactt cctttaatgg cagaaggatt atttgtaaat aactgtgtca atggtttgcc
    15301 ggcaagaagc ataaaacttg atataaggat cattagaact aagccaatat aatacgaact
    15361 ttgaatagtt tcttctatag attctctatt tttttgtcct acatattttg cagttaggat
    15421 aattgtagca gttgcgatgc caagtcctgg catataatta aattgtgtca aggtttctcc
    15481 aatagcattt cccgctacaa catttgtacc taactgaaca acaatagcaa ctataacaac
    15541 atctccagct cgcatcatca gacgttctcc tgctgatggt aaggcaagat tccaaatttg
    15601 tgcactgatt ttccaagtca ttctttttat tattttttta ataggaagat acttcgcaag
    15661 gatacaaata ccaattaatc ttgcgataag agttgatact gccactccga ccaaaccata
    15721 gcgccactca aaaatagcaa aacctgataa gactgcattg aggaaattga caaaaatact
    15781 gacatacatt ggtaaacgag gttgcccttg cacacgtaaa aatgagccta gggtggttaa
    15841 tattcctaaa gtaacaacac ctccacccac aattgctaaa tataaaccac caacttgtgc
    15901 gacacttttt gttgtcccta aaagtttaaa gaaagtttgt ccaaaaacaa tcgagataat
    15961 tcctaatact gcccctatca gtgatgttag aaaaatggct tgagaacata ctgaaattgc
    16021 atcatccttc tcactacctg ctaacgactt ggccaataga cttgctatac tcgcccctaa
    16081 agctataaaa atagcttgat aaatagtaat tatattatta gcaactgaaa cacctgatac
    16141 tgctacaaca cctaactgag ccactagata attatcaact actcccatga gcatttgtaa
    16201 gatattttca atcatagctg gtatagctaa ctgaataatc tctttactat aacgcacagt
    16261 atctcctttc tattacccag gatcattaaa aagagagtga tgggaaaatt tctaaaatgt
    16321 ttctttctcg ctctaaatat tttaaaacaa tcgtattttt attattgttt caaacctaga
    16381 cactcttcaa ccgtttctcg catatttgag gtaggtacta ccattctatg gattacttcg
    16441 gccttttgta gatctgtaac cgccttaggt aaagaaacac ctgacaaatc gtttaagatt
    16501 gaaatagcag cgaaatcctg tatctcttct ttatttgtaa tagcctttgt aacaacacac
    16561 gggaacttat aaggacttgc tgttgacaca attactgttg gagtttgatc tttagtttct
    16621 gtctgataag cttgataaac agctgatgct acagctgtat gcggatcctc tatatactga
    16681 tactggtcaa ataaacgttt gatgtctaat tcaacaaatt gttctgtcgc aaatcccgct
    16741 acaaagctct ctaaaatatt tgcttgccta gcctctaagg catactcccc agtagtcact
    16801 aaatcttcca ttaatttttt agtcgtttct gcatcatcgc ctaaaaggtg aaagattaat
    16861 cgttctagat tagaggagac caaaatatcc atagagggac ttgatgtcac tttaaattct
    16921 cgatttttat cgtaggtttg tgtcttaaaa aaatctgtta gaacgttatt atcatttgag
    16981 gcacaaatga gtttggtaac aggtaatcca atttgactag cataatatgc tgctaaaata
    17041 ttaccaaaat ttcctgtagg tacactaaaa ttaattagtt gaccaattga gatttccttt
    17101 gacttaacta attgagcata tgcatatata tagtaaacaa tttgaggaac aaggcgaccg
    17161 atgttcattg aattggctga tgataactgc atatgctgct gtgataattt tgatcgaaga
    17221 agggaattat tgaacatttc tttaaccgaa gtctgtgcat catcaaaatt tccttcaatc
    17281 gcaacaacat gggtattttg cccggcttgt gttatcatct gcaattcttg aatataacta
    17341 acaccatgtt taggataaaa tacaataatc tcagttcctg gaacatctgc aaaacctgcc
    17401 atcgctgcct taccagtgtc acctgatgtt gctgttaaaa taacgatttt atcatcaacc
    17461 ccttgttttt tggctgctgt tgttagcaaa taaggaagaa tggataaagc catatcttta
    17521 aatgcaattg taggaccgtg aaataattca aggtgatacc ggtctccaat ctttactatt
    17581 ggggctattt cagttgtatc aaatttagta tcataagctt gactaataca gtaatccaat
    17641 tcttgttcag taaaatcatc aaaaaaagct gacaaaacga gtttagccac ttcttggtag
    17701 gaagcatcct taagtttagt gaaatctaaa tcaacttttg gaaatgtgat gggggtaaag
    17761 agtcctccat cgtcagctaa tccctttaga atcgcttgac tagctgtcac tttattacta
    17821 gcatcacgtg ttgattgata tactattgcc ataagaatcc ctctttatat aaatatcttt
    17881 tagttattgt aacacaattt aataacatgt ccattgttcg ttatataatt aacccccctt
    17941 tattaaaata aaggggggcg taatatcatt atcactttgt aaagtcgaga actttacgtc
    18001 cttgaatcaa tcctctttcc atttcatcga atacttgagg agctgtatcc acaggcactt
    18061 tttcaaccac tggtactacc aaaccttctg ctccaaatgc gaaggcttct tctaaatctt
    18121 tacgagttcc aactaaggaa ccaaccacac gaatcccatc aagtactgtt tttacaatac
    18181 tcaattccat atattctgat ggaagaccaa cagctaccac tgtacctcca gcacgaacgc
    18241 tgtcaatcgc ctgattaaac gcaactttag aaacagcagt cacaaccact ccatgacaac
    18301 cgcctgtctt ttcttgaata taggccgcaa catcttttat ttctttgcca ttaactgtca
    18361 aatctgctcc aacctcttta gctaattgaa gtttatctgc gttaatatca acagctacaa
    18421 catgagcatt gaatactttt tttgcatatt ggactgctaa gtttccaaga ccacctgcac
    18481 catacactgc aatccactga ccaggagcag ctccagcttc tttgatagcc ttgtatgttg
    18541 ttactccagc acaagtgatt gatgatgctt gagctggatc taatccctca ggaaccttaa
    18601 ccgcatagtc cgcggtaacg atagcgtatt cactcatacc accatcaaca ctgtatccag
    18661 catttttaac actacgacaa agcgtctcac gtcctgtagt acagtattcg caatgaccgc
    18721 acccttcaaa gaaccatgca atagagacac gatcaccaac cctcaaagac gttacgcctt
    18781 ctccaatttc ttctactata ccaatgcctt cgtgacccaa aatgcgtcct ggtacctggc
    18841 cgaaatctcc atgtgcaacg tgtaaatcag tatggcacac accacagtat tctactttta
    18901 caagtgcttc accatgacca acttttggaa gatcatgctc aacaacttca acacctgtac
    18961 tagcttgatt aacaacaacc gctttcatga taaaacctcc tagaaataaa tatatcttat
    19021 ttgtaatcgt ttacatttta tattgtatta ttattcacaa actatgtcaa gtgattttca
    19081 gaaaattttt tgaaaatatt attaactggt taatcttttt gaaactaaaa atcccactca
    19141 atagagtgag attcttcgac tgttgttact tacggcgtcc tggacgttcc tcataaccat
    19201 aataagcatc ttcgatgatt tcttgcatgt gatctaccat tggaaggcgt ggatttgctg
    19261 gtgaacattg atcttcatat gctaagaaag ctagttcacg agatttttct ttccattctt
    19321 tttcatcaat gccttggtca cggaaattca tcttaatacc taaacgtgtt ccaaggtcat
    19381 acacagcttt tgcatatgat tcgacagctt cttctggtgt tgctgctggt aagccgagta
    19441 atttagcgat atcttgatac ttttcatcag cacgatagta attgtactta ggccatgttg
    19501 ctgttttagc tggacgagtt ccattatagc ggataacata cggcaaaaga attgcatttg
    19561 tacgtccgtg tacggtatgg aattgtgcgc caattttatg cgccattgag tgagaaatac
    19621 ctaagaatgc attggcgaaa gccatacctg ccatagttga cgcattgtgc atcttttcac
    19681 gtgcttcaaa atcagcatct tttactgagc gttctaagta gtcaaataca attttaattg
    19741 cttgaagagc aagtccgtct gtataatcat ttgccatctg tgaaacataa gcctctgttg
    19801 catgtgtcaa tacatccata cccgtatcag ccgcaataaa accaggaaca gtcataacaa
    19861 gggccggatc aacgatagct acagttggtg ttaatgaata atcagcgatt ggatatttac
    19921 gattatttgc tttgtcagag ataacggcaa atggtgtcac ttcagaacct gttccagaag
    19981 ttgttggaat agcaacaaat tttgttttct taccaagttc tgggaatttg aacgcacgtt
    20041 tacggatatc catgaatttt tgaacgaggt catggaaatc aacttctggt tgttcataga
    20101 agagccacat aactttcgca gcatccatag gagaaccacc acctaatgca ataatagtgt
    20161 ctggtttgaa ggtacgcatt aaatcagtcc ctttcattac tgttgtaatg tctggatctg
    20221 gttctacttc agcaaaaatc tgataaacaa ctttattgcg acgaagatca agttgttcaa
    20281 tgatacgatc caagaaacca agctcaacca tggcgtgatc tgtaacaatc atgacacgtt
    20341 caacatcgcg acatttttgc aaatattgaa tagaatcacg ttcgaagtat gtttttgaag
    20401 ggactttaaa ccattgcata ttatttctac gtcttcctac ttttttgata ttcaagagat
    20461 ttatagcact aacgttatca ccaactgagt tacgtccata cgaaccacaa ccaagagtca
    20521 atgatggcaa gaaagcattg taaacatccc caataccacc aaaagtagaa ggagaattcc
    20581 aaataacacg gatagcacga attctagttc caaattcacg agctaagtct gcatcttttg
    20641 tatgaatagc agctgagtgc cctagaccat taaactcaac catttgacga gctttttcaa
    20701 caccatcttc tgtagattca gctttcaata ctgcaataac aggtgatagt ttttcacgtg
    20761 tcaaaggttc tttttcacta acttcagtac attctgcggc aagaatattt gttccttctg
    20821 gaacagtaaa tccagcttgt tcagcaatcc aaactgctga ttttccaaca atatttggat
    20881 ttaattttgc accagcacag tttttgctat ttgcttttgc accaaaacag aattcttcaa
    20941 gaagagcttt ttctttctta ttaacgaaat aggtatgata agatttaaat tcttcaacaa
    21001 attctttata aatttcttta tcaataataa cagcctgttc tgacgcacat accataccgt
    21061 tatcaaatga tttagacata acaatatcgt gagcagcttg acggatatta gctgattttt
    21121 caacataagc tggaacattc ccagcaccta caccaagggc aggtttccca catgagtagg
    21181 cagctttaac catcgcatta ccacctgtcg caaggatagt ggcaatacca tcatgtttca
    21241 tcaaagcatt agttgcatca atcgatggtt gttcaatcca ttggacacaa ttttcaggag
    21301 ctccagcagc gatagcagca tcacgaacaa tacgtgctgc atgtgctgat gattcttgtg
    21361 ctgatgggtg gaaggcaaaa ataattggat tacgtgtttt aagagagatt aatgacttga
    21421 aaatagcagt tgaagtcggg ttggttgttg gcgtaatacc acagacaact cccactggtt
    21481 cagcaattaa tgtcaagcct gtgacatcgt cttcttcaat aactcccact gtctttgtat
    21541 gacgcatgtt gttaacgacg tgttcacatg caaataaatt ttttgtcgct ttatcttcaa
    21601 agacaccacg accagtttct tctactgcat gaagagcaag ttctccatgg gcatccaagg
    21661 ctgcaacaga agctttagct acaatataat caacttgttc ttgattaagt tgacgcatct
    21721 catcaagtgc ttttaaaccg ttttgaacta atgtatcaat tgcaagagca acatctgtag
    21781 tttctaccgc tttagttttt tcagtcattt gagactcctt ttgatttgtt aaatgtttca
    21841 caaatttgtt tacgagatta ttatagcata tttaaaaact ttgtaaatag tttcacaact
    21901 tttttgtgaa ctttttttca atctttcgga aacatttctt gaaagcgctt taattaaatt
    21961 tgttttttca ttactaattt tcagattaaa gccaatcgat ttcatttgta tcaccataaa
    22021 cggaggtatc tattattcgt tttcacaaaa aagccgagta catgactcgg acttgtttat
    22081 tttgcagctc gttgagctga tttttgaata gcttttttga cgttactaac atataaatca
    22141 gcccctgtag tatcgctatc actataatgc acaccatctg tatttcccca gatttctgga
    22201 tgttctgtcg caaccttata ccagtcagca accgttatgt aattatattt tttcattagc
    22261 gagagttcat aatcacgcac tgtcgtaact tgactcatat ttttagcatt ataaggcgtt
    22321 actataatca aacggtgtcc cttaggcaaa gatttaataa attggctcaa gtctgtcttg
    22381 tagttatcta atgaattcac accaactgcc aaaacaacaa ttttggacaa ggctttattt
    22441 tgaatacggt tttcaaaaat atcaaatgat ttactaaaat tacgactcac cgcagcatct
    22501 aattgtactt ctggtaacaa tttattaaaa gcagcacttg accttaaggc aactgaatct
    22561 ccgattaccg tcacatcact aagagcacct gcatcccctg cggctagtgt atgtgttcgg
    22621 ttagtatttg ttctagcttg ttgtaacgaa ttctgcaata attctgtctc aaaacttcct
    22681 atacttggag ctgtcaacat tgtcactaca gtaataagag ttagtacccc tccgatacta
    22741 aataaccact ttttgtaagg caacaagtca aattcataat caaggaattt aggttttcta
    22801 cctaaaataa aaggttcaat gatataaaat gataatgttg agaagactgc tgataatact
    22861 actgtcaaaa taacagctat ccagtttggg gataaacgac taaaaataat ataaaatggc
    22921 caatgaaaaa ggtacatacc gtaactgata tcagcaagat aagtaatcac gaaaggttct
    22981 gaaatatctg gtgtatgctc gtgtaaaata cgtgcattgt aaatcattac actagcaaaa
    23041 atgctagaca aaacaaaacc aaaaagatat gttagccgat tatcaaaatc aagtgcaaaa
    23101 gttaaaatca ttagaccagc aaaagcacta cccatcatga ttaaattatg cttcaaagtc
    23161 agatttttga tatttttctt gaatcgtccg gtaatctctc gaataccaga tattgtagcc
    23221 accatcgctc ctaaaaagaa cggaaatata tgtgataatg tagagaaata tatcgttgaa
    23281 aaattatcaa cgaaaaaggc tctaacaaac atggtaagga agctgactcc aaaaactccc
    23341 atggaaacaa ggaacaaagt ccccctcaat tgcttttgat cttttgatct tttcgataag
    23401 agccataccg ttaagcccca taaaacataa aaatggactt caatagaaag actccaagta
    23461 tgcacaaata agtgagggat aaattgactt tcgtagttac caccagtcaa gatttcgtaa
    23521 aagttgctgg taaaacctaa agcagtcata atttgagagc caatacttgc tctaaaatcg
    23581 cttttaacca gaaatacaaa aggtattgtc accaaaacca tcaatactaa tggtgggaaa
    23641 atacggtaaa aacgtctacg acaaaaacta acaaaatcaa tttttttcgt tttagaaaat
    23701 tcatcaatca acaaggccgt aatcagaaaa ccagaaaatg taaagaagat gtcaacacca
    23761 acaaatcctc ctggaaaact attcttaaag aaatggtaaa gtaaaacaag tagtagtcct
    23821 gttatacgta ccaaagaaaa ccattttatt ctcatttttg ataaatcccc tgtttaaatg
    23881 tccccttata gactttctta tttttagcat tgaggcgacc ttgtccatca ggttgacctt
    23941 ttttaaagtc acctgtataa gaccagccat gaactgaaac aaatgttcct ttcccttcaa
    24001 agaccccatt tacaaaatcg cccttatagt aatccccatt ctcataggtt aatttacctt
    24061 taccagtcat atggtgatta acaatagagc ccacatattt tatcttaccg ccatcatagc
    24121 taaggactcc ttgactagaa gtcgttaagg taaatactga taaaccaaaa acaacaataa
    24181 ttatcaaaga taatatttct agatgagttc gtgttatttt aaaattcttc atttctactt
    24241 aatctctact attatttatt agcgttaacc actgtttaca gccatcactg actagacgat
    24301 aggtttcctc aaaatcacca gtataccatg gatcaggaac accaccatca caaaaaaggc
    24361 taatcttatc atcataggtt ccttgtgata gagcttttaa atccttgaca ttttgctcat
    24421 ccataccgat gatataatca aaatatgcta aatcgtcttt agaaataggt tgtgaccctt
    24481 tatcgttagt gtaaggaatt tgataacgct gcaaaatcgc ttgcgttccc ctatgtatag
    24541 gatttccatg ctcccatgtt gaagtcgcgc gactctcaac cgagaggcta tcctccttat
    24601 ctaaattcgc cataacaaac tcagccatag ggctacggca gatatttcca agacaaacaa
    24661 aacagacttt cttcatagca aactcctttt ggtctattat aacagaaaaa aggtacttat
    24721 aatacgttta actgctcaaa gaataactac aacatatcta atacttaaca ttgcatgaaa
    24781 attaaaaaat taagactaga taccctagcc ttaaaatgac ttatgtttta tttatcaaat
    24841 cgctgataac ctaaatgttc gtatgcctta tccgtagcta cacgaccggt acgggtacgc
    24901 ataataaaac ctttttgaat taaataaggt tcgtacatgt cttcaacagt atctcgttct
    24961 tcagtaatat taacggatag agttcctaaa ccaacaggac ctccattata catttcaatc
    25021 atggttctta agattttttg atcgacgtaa tcaagcccct cgtgatcaac atctaacatc
    25081 gttaatgctt tatctgtaat attgtcatct atcaaaccat ctcccatgat ttgagcataa
    25141 tctcgaacac gtttcaataa acggttagcg atacgtggcg ttccacgact gcgacgcgct
    25201 aattcagaag cagcttcata agtaattttc atttcaaaaa tatctgctgt acgctcaata
    25261 atttctgtca aatcgttttc ttcataatac tccatatgcc ctgtaatacc aaagcgagca
    25321 cgtaagggat tagataacat acctgcacgt gtcgttgcac caattaaggt aaagggcggc
    25381 aaatctaaat gaacacttct actagtttct cctgcaccga tcataatgtc aatatagaag
    25441 tcttccattg cactataaag tacctcttca accgccatcg gcatacgatg aatttcatca
    25501 ataaaaagaa catcacctgg ttctaaatca tttaaaatgg ctactaaatc ccctgatttt
    25561 tcaattgcgg gacctgatgt ttgtttgaga ttgacaccca actcattagc aattacaaat
    25621 gccatggttg ttttccctaa accaggaggg ccaaataata acacatgatc caatgactca
    25681 tcacgcaatt tagcagcttc aataaatatt tttagctgat ctttaacctt atcttgtcca
    25741 atatactctc ttaaatactg cggacgaagt gtacgttcta ccaattcttc gtcacccatt
    25801 gcatcactat ctaaaaatct tgtcatgata ttattataac atttgtatag gaaaagttaa
    25861 gaactaagtt ggtgtattag tatctccctt atttaaagga agaataaact gatcttatag
    25921 ttaatccaac ctcaagaaat cacagaacag cttacttagc ccatattcca ccaacccatt
    25981 cctaaaaaca aatcaaatga acctaaattt gataatagaa caagtaatac tattaaaaaa
    26041 cattttttaa tcactataat aaactcctaa ataatatttt gtacgtttat tataaatttt
    26101 tagacctcag agtcacagga catcaatgtc ctgttatatc ttcttgccac tccaattcta
    26161 attttttaat tagatgcttg tcatcaatca tttttgaaaa cataattgat tgttggtaag
    26221 cattttctgc agactttata tcattgtcaa ttgtaatgaa gtatttccat tggtacataa
    26281 aatataaagg cattttatga aaatcatgtg ttgacaccat cgtttgtcgg ctaacttcta
    26341 acatttctgc aagacactct tcactattta aacaaagtcc aacaaataca caattcagga
    26401 gtacatgatt aaaccaaaac aaatcttctg ctgtaagttt gtggttctga cttatcaaag
    26461 ttttacatat agtttgaaat acttctttag taaatgctct attgttaaat ttagatacaa
    26521 ctgcacaagt taaaaacaaa tctatgataa tcaaatcatt aagagtatat ttttctttat
    26581 attttacttt atcaaagcat tcgcataaaa cctccacacc aaaattaatg tctcctgttt
    26641 gatagacttc aaatttagct tgtaaactgt caattgctaa gcactcgtct tctggaagat
    26701 tatcatagaa ttcttcgaaa atatgatcaa actgacattc tcttaatttt aatcgttcag
    26761 catctgcata agttggaatt cttaaaatca aatacttcaa ctctttatac cgtttaggta
    26821 attctaagct ctctcctcct gttaaaatat ctaattttac attcaattgt ttagctatgt
    26881 agtgagcttt agtcaagttt gggattgact gtccactttc aatcctagct aattgtctta
    26941 cagataactc agtttcatca ccacaaaaat cttcgcgagt aatatttcta gttactctca
    27001 aatttcttac tttactacca aaatgtttta acattttcct ttaaaaacta actcctaaat
    27061 ttttcttctt attatatatt atcataataa actcatttta agtatgtttt tttaatatta
    27121 attcctttta aatcaataaa aattccatta ctggttaaaa aaacgttaac cagtaatgga
    27181 attttcttat ttttctaatc ctagtctttc aaaaatatca tcaacacgtt ttgtgtaata
    27241 aatcggatta aatagttcat caatttcttc ctgtgtaaga caagaggtaa ctttggtgtc
    27301 ttcttctaaa agtggtttga aatccacttg attgtcccag gaataagctg tcttaggttg
    27361 aactaagtca taagcttctt ctcgtgtcat tcctttttca atcaatttga gcataacacg
    27421 ttgactataa atcaaaccaa aagttgattc catattgcgc atcatatttt ccgggaagac
    27481 agtcaagttc ttaacgatat tgccaaaacg gttgagcatg tagtcaatca agatagttgt
    27541 gtctggtgtg atgatacgct cagctgatga gtgcgaaatg tcacgctcat gccaaagtga
    27601 cacgttctca taagctgtca ccatgtgacc acgaatcacg cgcgctagcc ctgtcatgtt
    27661 ctctgaaccg attgggttgc gtttgtgagg catagcagag ctacctttct gacctttggc
    27721 aaagaattct tcaacttcac gttgttctga tttttgcaga ccacgaatct ctgtcgccat
    27781 acgttcgata gatgttgcga tgcttgcaag tactgcaaaa tattctgcgt ggaggtcacg
    27841 tggaagaacc tgtgttgaaa tttcttgcgg acggatgccc aatttgtcac agacatattt
    27901 ttccacaaat ggaggaatgt tggcaaagtt accaacggca cctgaaatct taccagcttc
    27961 cacacctgcg gcagcatgtt caaaacgctc aatattacgt ttcatctcgc tgtaccaagt
    28021 cgccaacttg agaccgaaag tcgttggctc ggcgtgaaca ccgtgggtac gacccatcat
    28081 gatggtgaac ttgtgctcct tagccttgtc agctacgatg tttgtgaaat tctcaagatc
    28141 acgacggata atgtcgttag cctgcttgta gaggtaaccg taggcagtgt ccaccacgtc
    28201 agtcgacgtc aaaccgtagt gcacccactt gcgctcctca ccaagcgtct cagaaaccgc
    28261 acgagtgaaa gccaccacat cgtgacgcgt gtcctgctca atctcaagaa tgcggtcaat
    28321 gtcaaaatcc gccttctcgc gaatcttagc cacatcctct ttaggaatct cacccaactc
    28381 agcccatgcc tcgtcagcca aaatctcgac ctccaaccaa gcacggtatt tattttcctc
    28441 tgtccaaatt gccgccatct cagggcgtga ataacgtgaa atcattgttt gtctccttta
    28501 acttatttca ttaaaatact agaaatgaca ttgcctaaca ccccagttat ggtattctga
    28561 acataatcat ttttgaagaa actagacatt ttattgacaa aatttttcaa ttttctttca
    28621 tcctctgcct cactaatttc caacaattct gaatatatcg ctttatccgc agtagatagc
    28681 tgagtgttaa taattaaatt taactgctca accatatcag tccatggaat ttccacactt
    28741 ttatgtatat
//
LOCUS       DATRME010000017        27523 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRME010000017 DATRME010000000
VERSION     DATRME010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27523
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..158
                     /locus_tag="VBC23_000776"
     CDS             <1..158
                     /locus_tag="VBC23_000776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842742.1"
                     /translation="TYKEDYQKDKGLHEINLFNEEKSVFYGEKIIFETDLSDYIINQI
                     EKKLLLR"
     gene            199..996
                     /locus_tag="VBC23_000777"
     CDS             199..996
                     /locus_tag="VBC23_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEO6842743.1"
                     /translation="MKIITFAAIKGGVGKTTLAYNYAEWLAEQNYQVLLIDLDHQCNL
                     TQTYDVFESENTVANIFKRKGDVNIHHLKDNIDLIAGYIRLDSLEKELETKNYKDMLL
                     YMWLEDNYESKNLGKYDFILIDCHPDFSTATRNAVVVSHAVLSPVVPSKFGYSATFNI
                     KERLEEFKAETFDFKTRKSYVTADLYFLGNMIKHNTNSSRELVKQLQDNDNVLSTIPY
                     KELFNRSTLDKISLSEMKQDKALYQKHRKFFDSIDCTFKEITDKIAV"
     gene            1064..1288
                     /locus_tag="VBC23_000778"
     CDS             1064..1288
                     /locus_tag="VBC23_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842744.1"
                     /translation="MAFDNNQEKISTLLKSTETTTKNNTNSNFEFERTKQFMFSLQPS
                     VREKITQIAHNRGYRSASSFLNDYFKNFEL"
     gene            1408..2232
                     /locus_tag="VBC23_000779"
     CDS             1408..2232
                     /locus_tag="VBC23_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEO6842745.1"
                     /translation="MDLITRLKVERDGRVYGGIYDITQKRFAFNSNKIEGSRLTEEQT
                     SFIYETKTIANIGGTGIKIDDLVETTNHFKCFDYIINTVDEQLTEDYVKKLHSILKAG
                     TSSEYNEYVPVGRYKVFENEVGQIATAAVDQVEEEMYSLLLGYNFKKEKDLAYLASFY
                     ARFEQIHPFADGNGRVGRLILYKECLKEGITPFIVDDTRKADYYNALEQFQVYDNHQP
                     LIDYFAIEQKFYANYLKNKGFVGKINDSKDRDVINKKSKSADDSHKRPNKTKGFSL"
     gene            2314..3663
                     /locus_tag="VBC23_000780"
     CDS             2314..3663
                     /locus_tag="VBC23_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO6842746.1"
                     /translation="MTGMRFDEKVFVVELDDKRKYDFLKRVKTYDANIASAMRIAGYK
                     RINQTERTVAFTFGEVTFSRSRWRKGNKTRCPIDEWLGLKKYMRFSPELICHIAKMAT
                     VMSYRQVCHFVKEFYHLEITKDTVLKAVKRAGDLLSEKEKFRFWLEEEPTKKIKPKIL
                     YIEGDGVMVKSSDSARDDKRNVDLTHFLVHTGSKKIAQNRYVLENKHEIIHTNYEKAK
                     EELLDYLYNHYEITDETILITNSDNGKGYTPKVFQEIKKALGIKRHEHFWDAFHLHEK
                     IKIFFKKYPAELKELAFEAINTHKRELMKTVLDTTESLIDGEGDEYYNYFNFRRKILN
                     NFKYTKPAKLRNLSSKGIGVMESQHRKITYRMKHRGMYWSLKGAYTMSRLIILERINL
                     VDDLFFGDWRKEYQYYQNQRFSVGYLKEIKAETIIKTRKIFQRAGKFTSLDRQKFKY"
     gene            4085..4219
                     /gene="rpmH"
                     /locus_tag="VBC23_000781"
     CDS             4085..4219
                     /gene="rpmH"
                     /locus_tag="VBC23_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6842747.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            4575..5249
                     /locus_tag="VBC23_000782"
     CDS             4575..5249
                     /locus_tag="VBC23_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842748.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            5249..6436
                     /locus_tag="VBC23_000783"
     CDS             5249..6436
                     /locus_tag="VBC23_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6842749.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            6447..6569
                     /locus_tag="VBC23_000784"
     CDS             6447..6569
                     /locus_tag="VBC23_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6842750.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            6582..8117
                     /gene="dltA"
                     /locus_tag="VBC23_000785"
     CDS             6582..8117
                     /gene="dltA"
                     /locus_tag="VBC23_000785"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6842751.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            8114..9379
                     /gene="dltB"
                     /locus_tag="VBC23_000786"
     CDS             8114..9379
                     /gene="dltB"
                     /locus_tag="VBC23_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6842752.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            9394..9633
                     /gene="dltC"
                     /locus_tag="VBC23_000787"
     CDS             9394..9633
                     /gene="dltC"
                     /locus_tag="VBC23_000787"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6842753.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            9626..10888
                     /gene="dltD"
                     /locus_tag="VBC23_000788"
     CDS             9626..10888
                     /gene="dltD"
                     /locus_tag="VBC23_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6842754.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
     gene            11063..11455
                     /locus_tag="VBC23_000789"
     CDS             11063..11455
                     /locus_tag="VBC23_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6842755.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            11455..12747
                     /locus_tag="VBC23_000790"
     CDS             11455..12747
                     /locus_tag="VBC23_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6842756.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            12738..13130
                     /locus_tag="VBC23_000791"
     CDS             12738..13130
                     /locus_tag="VBC23_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842757.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            13140..13421
                     /locus_tag="VBC23_000792"
     CDS             13140..13421
                     /locus_tag="VBC23_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842758.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            13603..14385
                     /locus_tag="VBC23_000793"
     CDS             13603..14385
                     /locus_tag="VBC23_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6842759.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            14372..14932
                     /gene="rnmV"
                     /locus_tag="VBC23_000794"
     CDS             14372..14932
                     /gene="rnmV"
                     /locus_tag="VBC23_000794"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6842760.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            14951..15442
                     /locus_tag="VBC23_000795"
     CDS             14951..15442
                     /locus_tag="VBC23_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842761.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            15522..16394
                     /gene="rsmA"
                     /locus_tag="VBC23_000796"
     CDS             15522..16394
                     /gene="rsmA"
                     /locus_tag="VBC23_000796"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6842762.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            16398..17249
                     /locus_tag="VBC23_000797"
     CDS             16398..17249
                     /locus_tag="VBC23_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6842763.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            17405..18277
                     /gene="rsgA"
                     /locus_tag="VBC23_000798"
     CDS             17405..18277
                     /gene="rsgA"
                     /locus_tag="VBC23_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6842764.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            18284..18946
                     /locus_tag="VBC23_000799"
     CDS             18284..18946
                     /locus_tag="VBC23_000799"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6842765.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            18939..19571
                     /locus_tag="VBC23_000800"
     CDS             18939..19571
                     /locus_tag="VBC23_000800"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6842766.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            19573..20847
                     /locus_tag="VBC23_000801"
     CDS             19573..20847
                     /locus_tag="VBC23_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6842767.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            20837..21778
                     /locus_tag="VBC23_000802"
     CDS             20837..21778
                     /locus_tag="VBC23_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6842768.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            21875..22687
                     /gene="purR"
                     /locus_tag="VBC23_000803"
     CDS             21875..22687
                     /gene="purR"
                     /locus_tag="VBC23_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6842769.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            22906..23319
                     /gene="rpsL"
                     /locus_tag="VBC23_000804"
     CDS             22906..23319
                     /gene="rpsL"
                     /locus_tag="VBC23_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6842770.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            23341..23811
                     /gene="rpsG"
                     /locus_tag="VBC23_000805"
     CDS             23341..23811
                     /gene="rpsG"
                     /locus_tag="VBC23_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6842771.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            23966..26044
                     /gene="fusA"
                     /locus_tag="VBC23_000806"
     CDS             23966..26044
                     /gene="fusA"
                     /locus_tag="VBC23_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6842772.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            26248..27258
                     /gene="gap"
                     /locus_tag="VBC23_000807"
     CDS             26248..27258
                     /gene="gap"
                     /locus_tag="VBC23_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6842773.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
BASE COUNT         9356 a         4297 c         5412 g         8458 t
ORIGIN      
        1 atacttataa agaagattat caaaaagata agggattaca tgaaattaat ctttttaacg
       61 aagaaaaatc agtcttctac ggtgaaaaaa ttatttttga gacggatcta agcgactata
      121 ttatcaatca aattgagaaa aagttgttgt tgagataata caaatataaa tatattgaaa
      181 aagtaaagga gaaataacat gaaaatcatt acatttgctg ctattaaagg tggtgtagga
      241 aaaacaacat tagcatataa ttatgctgaa tggttagccg aacaaaacta tcaagtgctt
      301 ttgattgatt tagatcatca atgtaatcta actcaaacgt atgatgtctt tgaaagtgag
      361 aatacagtcg ctaatatctt taaacgaaaa ggagacgtta atattcatca tcttaaagat
      421 aacattgatt taatagcagg ttatattcga ttagatagtc tagaaaaaga gcttgaaacc
      481 aaaaattata aagatatgct attgtacatg tggttagaag acaattatga atctaagaat
      541 cttggcaaat atgattttat tttgattgat tgtcatcccg atttttcaac agcaacacgt
      601 aacgctgttg tagtaagtca tgctgtttta agtcctgttg ttccgtcaaa atttgggtat
      661 tctgcaacat tcaatattaa agaacgtcta gaagaattta aagcagaaac atttgatttt
      721 aaaactcgta aaagctatgt tacagctgat ttatatttct taggtaatat gataaagcat
      781 aacactaatt cttcgagaga acttgtaaag caacttcaag ataacgataa tgttttatca
      841 actatccctt ataaagaatt attcaatcgt tcaactttag ataagatttc tctttcagaa
      901 atgaaacaag ataaagcact ttatcaaaag cacagaaaat tctttgatag tattgactgt
      961 acttttaaag aaattactga taaaatagca gtataaatat attgatattg atgattatat
     1021 ttatatttgt aaatataaat ttactgaaaa gatgggagaa aatatggcat ttgataataa
     1081 ccaagaaaaa attagcacac ttctaaaatc aaccgaaacc acaactaaaa ataacactaa
     1141 ttcaaatttt gagtttgaaa gaactaaaca gtttatgttt tcgttacaac catctgtacg
     1201 agaaaaaata acacaaattg ctcataatcg cggttatcga tctgcatcat catttctaaa
     1261 tgattatttt aaaaattttg aattataaaa aataatttta tttatggttt tacataaaat
     1321 agtatatata atcataaata tatttacaaa tatatttata tttaatgtta taaatatgaa
     1381 tatactgccc attaaaggag aataatcatg gatttaatta caagacttaa agttgaacga
     1441 gatggcagag tttatggtgg catttatgat attactcaga agcgttttgc atttaattca
     1501 aataagattg aaggcagtcg tttaactgaa gagcaaacga gtttcattta tgaaacaaaa
     1561 acaatcgcaa atattggtgg aacaggtatc aaaattgatg atcttgttga aactactaat
     1621 cattttaaat gttttgatta tattattaat acagttgatg agcagctaac agaagactac
     1681 gttaaaaaac tacatagcat tttaaaagct ggaacaagct cagaatataa tgaatatgtc
     1741 ccagttggac gttataaggt atttgaaaac gaagtaggac agattgctac agctgctgtt
     1801 gatcaagttg aagaggaaat gtactcacta cttttgggtt ataatttcaa aaaagaaaaa
     1861 gatttagctt atctagcatc tttctatgca cgttttgagc aaattcatcc gttcgctgat
     1921 ggtaatggtc gtgttggacg tttgatcctc tacaaggaat gtttaaaaga aggaataact
     1981 ccattcattg tagatgatac aagaaaagca gattattaca atgcactaga acaatttcaa
     2041 gtttatgata accatcaacc tttgattgat tattttgcga ttgaacaaaa attttatgca
     2101 aattacctta aaaacaaagg atttgtggga aaaataaacg atagcaaaga cagagatgtt
     2161 atcaataaaa aaagtaaaag tgctgatgat agccataaaa ggccaaacaa aacaaaaggt
     2221 ttttcgttat aaatatagtt atatttaaaa atcttcattt ccatgaggag ttttttgtgt
     2281 gatagtttat ccaaaattag gtaaaatacc tgtatgacag gtatgagatt tgatgaaaag
     2341 gtttttgttg ttgaattaga tgataaacgt aaatacgact ttttaaaaag agttaaaacg
     2401 tatgatgcaa atatcgcctc agcaatgcgg atagctggat acaaacgtat caatcaaaca
     2461 gagagaacgg ttgcttttac ttttggagaa gtaacattct ctaggtcacg ttggcgaaaa
     2521 ggaaataaaa cacgttgtcc tatagatgaa tggttagggc ttaaaaagta tatgcgtttt
     2581 tctcctgaat tgatttgcca tatagctaaa atggctacag tgatgtcgta tcgtcaagtg
     2641 tgtcattttg ttaaagaatt ttatcatctt gagattacta aggatactgt tttaaaagct
     2701 gttaagcgag ctggggattt gctgtctgaa aaagaaaagt ttcgcttttg gctagaggaa
     2761 gaacctacca agaaaattaa accaaaaatc ctttatattg aaggagatgg cgtgatggtg
     2821 aaatcatcag attctgctag agatgacaaa agaaatgttg atttaacgca ctttcttgtt
     2881 catactggtt ctaaaaaaat tgctcaaaat cgttatgtcc tagaaaataa acatgagata
     2941 atccatacaa attacgaaaa ggcaaaagaa gaattactag actatttgta taatcattac
     3001 gagatcacag atgagacgat attaatcact aattctgata atggtaaagg ctatactcca
     3061 aaagtttttc aagaaataaa aaaagcgctt ggtatcaaaa gacacgagca cttttgggat
     3121 gcttttcact tacatgaaaa gataaaaata ttttttaaaa aatatccagc tgaattaaaa
     3181 gaattagctt ttgaggcaat taatactcac aaaagagagt taatgaaaac agttcttgat
     3241 accacagagt ctttaatcga tggtgaaggt gatgaatact ataattattt taattttcgc
     3301 cgtaaaattc ttaataattt caaatatacg aagcctgcta aattaagaaa tttgtcttct
     3361 aaaggaatag gtgtcatgga aagtcagcat cgaaaaataa cctatcgaat gaaacacaga
     3421 ggtatgtatt ggtctttgaa aggggcatat acaatgtcca ggttaatcat tttagagaga
     3481 atcaatcttg ttgatgattt attttttggt gattggcgaa aagagtatca atactatcaa
     3541 aatcaacgtt ttagcgttgg ttaccttaag gagataaaag cagagacgat tataaaaaca
     3601 agaaaaatat ttcagcgtgc tgggaagttt acttcacttg atcgtcaaaa atttaagtat
     3661 taaagacttt aaaatttttt gattttaagg cttttttcag ctgttttcgt ttgcattttt
     3721 ctgacaattt tgttataata tcattgtaga aaatcaaaaa aggagcagaa gcggtaactt
     3781 ctactccacc gattaagaca aatctacact ttgcttaatc agtctgaagt tattgtaaca
     3841 cgaaaaagcc aataaagtca agactgaagc aaggaaacct ttgtttcggt atttttttga
     3901 aaaaataatt gagaaatctc ttgatttata ggctggtgag tattctcaaa aaattagaca
     3961 cttacaactt gaaagtattt cttgacaaga ggcctaaaat tgttataaaa tagtatggta
     4021 tgtttagtaa ctgatcaaaa tagccggcta aacgaatacg aaaatctatc aggaggtatt
     4081 caacgtgaaa cgtacttatc aaccaagtaa aatccgtcgc caacgtaaac acggattccg
     4141 tcaccgtatg tcaactaaaa acggacgtcg cgtgcttgct agccgccgtc gtaaaggacg
     4201 taaagtgtta tcagcttaat atagatagca aaataatatc aagccaacag gtactcgagt
     4261 ctggtggctt ttttggtacc ttcataacga taaaggtttt tgtgagagta gtttgagtat
     4321 attatagata aagttaatga aaagtgagtt tataatgcgg aaattcgtag acaaatagcc
     4381 cctatttttg ttagaatatg aggagatttc ttattgagaa atatctttat agcgagtgat
     4441 attttatttt ttagacgctt gtattattga agtagtttag tgacttagga aatactttta
     4501 ttaatatcgt tttttcaagg ttatttcaat tttgctagtt tatactataa tatataaggt
     4561 aaggtgacta gaaaatgaga cttcttgtag ttgaggatga aaaaagtata gcagaggcta
     4621 ttcaggcttt attggcagat aaaggttatt ctgttgattt agcatttgat ggagatgatg
     4681 ggttagaata cattttgaca ggtctctatg atttagtcct gttggatatt atgttaccca
     4741 aacgctcagg attatctgtt ttaaaaagag tgagggaagc tggtttagaa accccaataa
     4801 tctttttaac tgcaaaatca cagacttatg ataaagttaa tggtttagat ttaggggcag
     4861 atgattatat cacaaaaccc tttgaagcag atgaattgct agctagaatt aggctgagaa
     4921 ccagacaatc ttctctaatc agagcaaatc aattgcggct tggaaatata aggcttaaca
     4981 cagattcaca tgagctagaa tctaaagaaa gtagtgtaaa attatccaat aaagaatttc
     5041 tcttgatgga agtttttatg cgaaatgcaa agcagattat tcctaaaaat cagttgattt
     5101 ctaaggtttg gggaccttca gacaattcag aatataacca gttagaagta tttatttctt
     5161 ttttgcgaaa aaaattgcgc tttttaaaag cagacattga aataataacc actaagggct
     5221 ttggatattc cttggaggag aggacataat gtttagtgat ttacgcaaaa aatttgtctt
     5281 tttgaccatg tcaatcctta ttgtcgttgt tctctttcta tttgcagtta gtaatcgcta
     5341 caatcaatat tgggatgaat atgatgcata taggattgtt aaattagttg ccaaaaatga
     5401 ttatttagga ataccaggcg atgaaccgat tgctttggtg actatagata accaaaagat
     5461 ggttaagatt caaagcaata atactgattt gacaaatgat gttatcgaaa aatctagttt
     5521 gaagctcctt gagcaaggaa agaagtcacg aaaatggaag tcttttatat attccataaa
     5581 agaatataaa gataaaacct acaccatagc tattatggat ttggctagtt atgaagtccc
     5641 ttatgcaagg cgttttctaa ttcttgtttt tactattttt ggattttgtt tattagcagc
     5701 tgtaagtctt tatctttctc gatttattgt aggacctgta gagacagaaa tgactcgcga
     5761 aaaacagttc gtttcagatg cgagtcatga gctgaagacc cctattgcag ctatacgagc
     5821 caacgttcag gttctggaac agcaaattcc agggaatcgt tatctggatc atgttgtttc
     5881 ggagactaag cgaatggagt ttttaattga agatttgctt aatttgtcta gacttgatga
     5941 aaaacgctcc aaggtaaatt tcaaaaagtt aaacttatct gtcttatgtc aagaagtact
     6001 tctaacatat gaaagtttgg catatgaaga agaaaagtgt ttaaatgata cgattgaaga
     6061 tgatgtctgg attgttggag aagagagtca gattaagcaa attttaataa ttcttttgga
     6121 taatgctatt aggcattctt tgtcaaaatc agcaatccag tttagtctta aacaagctcg
     6181 aagaaaagct attctgacaa tatcaaatcc aagtgctatc tattccaaag aggttatgga
     6241 taatctcttt gagcgttttt atcaagctaa agatgatcat gcagattctc tcagttttgg
     6301 tctaggctta tctattgcta aagcaatcgt ggaacgccat aaggggagaa ttagagcata
     6361 tcaagaaaaa gaccaattac gtcttgaagt ccagttacca attgatggat tttggaccaa
     6421 taccatgatt aattaaagga agaatgatga aaccatattt atcttttatt ggtagaacgt
     6481 tattatactt cggtatttta ttgttactaa tttacttttt tgcatacctt ggtcgcggac
     6541 aaggcagttt tatttataat gaattctagg agatggtaca aatgatacat gatatgatta
     6601 aaacaattga gcattttgct gagacacaag ctgattttcc agtgtatgat attttagggg
     6661 aagtccatac ttatggacaa cttaaagtag actctgactc tctagctgct catattgata
     6721 gcctaggcct tgttgaaaaa tcacctgtct tagtattcgg tggtcaagaa tatgaaatgt
     6781 tggcgacatt tgttgcttta acaaagtcag ggcatgctta tataccggtt gaccaacact
     6841 ctgctttgga tagaatacag gctattatga cagttgctca accaagcctt atcatttcaa
     6901 ttggtgaatt tcctcttgaa gttgataatg tcccaatcct agacgtttct caagtttcag
     6961 ctatttttga agaaaagact ccttatgagg taacacattc tgttaaaggt gatgataatt
     7021 actatattat tttcacttca gggactactg gtttaccaaa aggtgtgcaa atttcacatg
     7081 acaatttatt gagctttaca aattggatga tttctgatga tgagttttca gttcctgaaa
     7141 gaccgcaaat gttggctcaa ccgccatatt catttgactt atcagttatg tattgggcac
     7201 caacactagc tatgggaggc accctgtttg ccctaccaaa aacagtagtt aatgatttca
     7261 aaaaactatt cgctaccatt aatgaattgc caatacaggt ttggacttcg acaccatcat
     7321 ttgctgatat ggcgctacta tctaacgatt tcaattcaga gaccttgcca cagttaacac
     7381 atttttattt tgatggggaa gagttaactg tcaagactgc acaaaaactt cgtcaacgtt
     7441 ttccaaaagc tcgtatcgtt aatgcatatg ggccaacaga agcaacagtt gctctatccg
     7501 cagtagcaat tactgatgaa atgttagaaa catgcaaacg ccttccaatt ggttacacta
     7561 aagatgactc accaacgtat gtgattgatg aagaaggtca taaattacca aacggagagc
     7621 aaggtgaaat cattattgct ggaccagcag tatcaaaagg ctatcttaac aatccagaaa
     7681 agacagctga ggcatttttc caattcgaag gtctacctgc ttaccatacc ggtgacttag
     7741 gaagtatgac cgatgaaggt cttctgcttt acggtgggcg tatggatttc caaattaaat
     7801 ttaacggcta tcgtattgaa ttagaagatg tttctcaaaa cttaaacaaa tcgcagtatg
     7861 taaaatcagc agtagcagtg ccacgttata acaaggatca taaagttcaa aacttattag
     7921 cctatattgt cttaaaagaa ggtgtaagag atgattttga acgtgatttg gatttgacaa
     7981 aagcaattaa ggaagactta aaggacatta tgatggatta catgatgcca tctaaattta
     8041 tctatcgaga ggatttacct ttgacaccaa atgggaaaat tgatatcaaa ggtcttatga
     8101 gcgaggtaaa caagtgatac aggcattttt agaaaaactt cctcacttgg atgtttatgg
     8161 gaatccacaa tatttcttct accttatctt agctgtccta ccaatttaca tcggcttatt
     8221 ctttaagaag cgttttgcct tatatgagat tatttttagt ctaagtttta ttgtaatgat
     8281 gttgactggt agtactttta atcaattgaa gtcactattg gcatacgttg tcggacagtc
     8341 tctgctagtt tttatctata aagcttaccg gaaacgattt aatcatactt tggtctttta
     8401 tgtaacggtt tgtttatcta tttttccgct atttttggta aaattaattc cagctatatc
     8461 tgaggatggg catcagtcac tttttgggtt tctaggaatt tcttacctta cttttagagc
     8521 tgttgctatg attattgaaa tgagagacgg tgtcttgaaa gaatttactt tatgggaatt
     8581 cttaagattt ttactcttct ttccaacttt ctcaagtgga ccaattgatc gttttaaacg
     8641 attcaatgag gattacatta atatcccaga tcgaaacgaa ctcctagata tgttaggtca
     8701 agcgattcat tatttgatgt taggttttct ctataagttt attttagcct atatttttgg
     8761 aagtctgatt atgcctcctc taaaagaatt agcgctagaa cagggtggtg tgtttaattg
     8821 gccaacactt ggggttatgt atgcctttgg ttttgatttg ttctttgatt ttgcaggtta
     8881 cacaatgttt gcgttggcta tttctaacct aatggggatt aagtctccga ttaactttga
     8941 caaacctttc aaatcacgcg acctaaaaga attttggaat agatggcata tgagcctttc
     9001 tttctggttt agagactttg ttttcatgag gcttgttaag cttttagtta aaaataaagt
     9061 ttttaaaaac cgtaatgtta cttcaagtgt agcttatatt atcaatatgc ttcttatggg
     9121 attctggcat gggttaactt ggtactatat agcctatggt ctctttcatg ggattggcct
     9181 agttattaat gacgcttggg tacgtaagaa gaaaaatatt aataaagaaa gaagattggc
     9241 taaaaaacca cttttaccag aaaacaaatg gacttatgct ttgggtgtct tcatcacctt
     9301 taatgtagtt atgttttctt tcttgatttt ttcaggattt ttagatcttt tgtggttccc
     9361 acaaccgcat aacaaataat aaggagtatt ataatggata ttaaatcaga agtattagca
     9421 attattgatg acctatttat ggaggatgtt tcttctatga tggatgaaga cttatttgac
     9481 gcaggagttc tagacagcat gggtactgtt gaactaattg ttgagttgga aagccacttt
     9541 aatattgata ttccgattgc ggaatttggt cgtaatgact ggaacacagc taataaaatt
     9601 gttgctggag taacggagct ctgcaatgct taaacgtcta ggtaaagtat ttggacctct
     9661 agtttgtgct ttactattgt tggtaggatt atattttgtt tttcctgttt ctcagcctca
     9721 tcatttaggt aaggaaaaaa acagtgcagt agcgttgacg aaggcaggtt ttaaaagcag
     9781 agttcaaaaa gttagagctt tcagtgatcc taaagccaat tttgtccctt tctttggttc
     9841 aagtgagtgg ctaagatttg acgcaatgca tccatcagtt ttagcagagg cttacaatag
     9901 gtcttatatc ccatatcttt taggtcaaaa aggggcggct tctctgacac aatactatgg
     9961 cattcaacag attaaaggac aaatcaaaaa taaaaaagct atctatgtta tttctccgca
    10021 atggtttgtt cgcaagggag ccaacaaagg tgcttttcaa aactatttca gcaacgatca
    10081 aaccattcga tttttgcaaa atcaaacagg gacaacctac gataggtatg ctgctcgtcg
    10141 attgttaaaa ttatatcctg aagcttctat gtcagatttg atagaaaaag ttgcagatgg
    10201 ccaaaaacta tcaaataaag acaaacaaag actaaagttt aatgattggg tatttgagaa
    10261 gacagatgct atttttagct atctaccact aggaaaaact tataatcagg caataatgcc
    10321 tcatgttggt aaattaccga aagcattctc atataatcat ttatcgcgta ttgcatcaca
    10381 agatgctaaa gtagcaacga gatcaaatca atttggtatt gatgatcgct tttaccaaac
    10441 aagaattaaa aagcacttaa aaaaattgaa agggtcacaa cgacatttca attatactaa
    10501 gtcaccagaa tttaatgatt tacagttggt tcttaatgaa ttctcaaaac aaaatacaga
    10561 tgtccttttt gtcataccac cagtaaataa aaagtggaca gactacacag ggcttgatca
    10621 aaaaatgtat caaaaatctg tagaaaaaat aaaacaccaa cttcaaagtc aaggtttcaa
    10681 tcatatcgct gacctttctc gagatggagg taagccatac tttatgcaag atacaatcca
    10741 tttaggttgg aatggttggt tagagctaga taagcatatc aatccatttt taacagagga
    10801 aaacagcaag ccgaattatc acattaataa taaatttttg aagaaatctt gggcaaaata
    10861 tacagaacgt ccaagtgatt acaagtaaat agtagagtca gatgatttat agcattagag
    10921 ctattaatca tcttttttaa tatctttgta tttggtaatc ctaagataac taatccatgt
    10981 tttatagttt ttcatttata atgagaataa atttatgata aactagatat caaatgagtg
    11041 ttatatgata aaaggaggag agatgaagtc aataaaatta cttagtttta ttgtaataat
    11101 ccttttaggg cttggtttca ctgtaggagg tttaaaagtg actgaaaaca ataaacaaca
    11161 agaattatgg gaaatagcta atagcaaaga cgctaaaaat gcttatcaaa agtggattta
    11221 tgcagaagat gaagatgctt tcaaagagaa tgctgttatt aagtcgtatg atattgacaa
    11281 agaaagtatc aaaaaaaatc ctatgggagg gatttcggta aggttaatca ttaataaaga
    11341 tcctaactta tatattactt gtaatttgga tagggataat caagggcatt tggtcagtca
    11401 aagtagccat caatctcccc agttaactca tttattggaa agtagaggtc attaatggaa
    11461 aaagtcttga ctgatttaga tagggtaaat atagcgaagc aagagtatga attaggtagc
    11521 caacttgata cactagtcaa aataatgagc caagataagg tactccccat cggtaaagtt
    11581 gctcacgttc aagatggagg caaagaaacc ggggaacaaa tttataccat cactccaaat
    11641 ggaactttag acaagccaga ggatgttaag gaagttacgg ttttatttaa ggggtcaaca
    11701 gcaccgtttg gaggggatga ttggaaaaca gactggttta aaaatgatat tccaatagct
    11761 agtaaactat tattaaagaa atttggttca caaagtgtat ctcataaaca aggtacaaag
    11821 cagttagaac aatcagctca tctgctaaaa gaagttatga ataaatatcc taacgcaaaa
    11881 atttcggtct acggacactc gttagggtca atgaatgcac agtatgctat tgcagaattg
    11941 gatgataatc aaatttaccg agttcaagga gcttggatat ataacggtcc aaacctttat
    12001 agtattttga cagataaaca aaaagaacag gttgataaaa taaaaggggt ggtcaataat
    12061 tacgttgatt ccaaggataa aattggaata ggttatccta aagaaggaag cgataatgct
    12121 gtcggaattg ttaaaatagt tgacagtaaa gctgtttcag gaggtagctg ggttgctaat
    12181 tttaccaatc agcatatttg gggaggctac cagtacaatc cagatggttc tttaaaatta
    12241 gcagaagata aatttgctta caaagaaaac tattcagtag ttcatacgaa tgtgaatcaa
    12301 agaatgcatg ctattaaagc gaaacttagt agctttaaaa gtacgggaaa agaagttagt
    12361 aagaatcaag ctattttcct agatagtcaa caagcaaaag tgatctctag tggtttggta
    12421 acgatatcaa aagaagcagt tgaagaagtt aaaaaagttg agcaaaaagc agttgctaaa
    12481 gcagaagagc tttatcaatc tttaaaagta gctcctacag gagttactga actaagtcct
    12541 caagaattgg aagaagcgta tgctcagtca ggtgttagct atgcgagcat tgtcggagaa
    12601 gctcaaacct ttttctcaaa taaaactcaa gcggcagaaa gcatttctca cgcttacgtt
    12661 gaattagaaa accaaattaa taaaggagtt gaagctatgt tgcgacaaga tagtaacttg
    12721 tcaggggagt ttaaacaatg gcactgattg atatcattga aaaacaatta gctgatactc
    12781 agagaaaaat atcagattta gatgatgcct atcaccatag ttgttgccag tttgaagaaa
    12841 aattagacga tctctcagta agaaaaaata agattattaa tatgcttcaa gaaacttacg
    12901 acgctgttga atatgatttg agatatagta atgatagctc agatatgatg acgctcaatc
    12961 gtattttagg ttcttatcat gacgacttag agcaagctta ccataaagag tactatgcct
    13021 tgtccgctca agaagaagaa tatagagcta actatattcg gcaacgttca gaacatgaac
    13081 taacctttga agagttgcaa agggaaaaga aaagagaatt aatgaagtag gagaaattat
    13141 tgagtttaat gtgggatgtc ctgactggta aggagtcagt aaggacttta aaaaaacaat
    13201 tggataaaga attgatacat agcgtgtacg cgccacgcaa gagatctaag tttcacgata
    13261 ttattacgtc gcatcataaa gaaatagacg aagtagccat caacacaaag acagctattc
    13321 ataaagttgc taatcaattg gacgaggcaa taaaaggaga atttcgttca aaggttgagg
    13381 aggctattga aagcaatcag caaaaatata atcatttata gtctagtttc tctaccatta
    13441 gctagacact tgtagaggat ttttacagca agtattcaaa agtcagagca aaaatgtttt
    13501 gacttttttg atttactctt tctgatcaga atgcttcagt cttatgttat aataataaag
    13561 attaatgctt gtagctacga taagcaaaga aagaacgatg atatgataaa aatatttgat
    13621 acccataccc accttaacgt agaaaatttt gaaggaaaga tagacgagga aattaattta
    13681 gctagcgaac tcggtgttac aaaaatgaat gttgtcggct ttgaccaaga taccatttca
    13741 aaatctttgg aattatcttc ccaatatgca caggtatata gcacaatagg atggcatcca
    13801 actgaagctg gttcttatga tgataatatt gagtcaatga ttatcagtca tttagaaaat
    13861 cccaaggtta ttgcacttgg tgaaattggt ctggattatt attggatgga ggatcccaaa
    13921 gatattcaga tcgaggtgtt taagcgtcaa attgaacttt caaaagagta taaccttcct
    13981 tttgtcgttc atacacgtga tgcgttagaa gacacttatg aagtcattaa ggaatctgga
    14041 gttgggcctt ttggtggtat aatgcactct ttttctggtt ctttggaaat ggctcagaaa
    14101 tttattgatt tagggatgat gatttccttt tctggtgttg tgacctttaa gaaggcatta
    14161 gatgtacaag aagctgcacg agagttgcca ttagataaga tattagtgga aacagatgct
    14221 ccttatttgg ctcctgtacc taaaagaggt cgagaaaaca aaaccgctta tacgagatat
    14281 gtcgttgaaa aaatagcaga gctacgaggc ataactgtgg aagaggtagc ggaggctaca
    14341 taccaaaatg cagtaaggat atttagactt gatgaaaaaa attgatattc aagaagtgat
    14401 cgttgtcgaa gggaaagatg atactgctaa tctgcgacgc ttttacaatg ttgataccta
    14461 tgaaactaga ggttcagcaa ttgatgaaga tgatttagag cgtatcgaac gtttgcacaa
    14521 tttgcgggga gttatcgtct ttacagaccc agattataat ggcgagcgta tccgtaaaat
    14581 catcatgaat gccattccga cagtccgcca tgccttcctc aatcgagacg aggcaaagcc
    14641 aggctcaaag accaagggac gatctctggg tgtcgagcat gccagctttg aagatttgca
    14701 gaaggcactc tcaaaagtga ctcagcattt cgatgatgaa gatcatttcg atatcaccca
    14761 agcagacctc attcgttggg gattcattac tgctagcgat agccgaaaac gccgtgagta
    14821 tcttgggaat caactccgta tcggctactc aaatggtaaa caactcctca aacgcctcag
    14881 actcttcgga gtgaccaagg cagaagttga agagtgtatg gaggggtatt aatagaaaag
    14941 gagaaaaata gtggagtata atgtaacgga agcattgact agagctgggg tagatttagc
    15001 aacgcttgca gtcaaaggga ctgcaactat ggtaaccagt aaaattcaat caataaagga
    15061 tgtaaaagat gctgcaacat tgagagctac ttatgatgat ttaattaacg aattattagc
    15121 tgaacgggag caagcaattc gcattgcaca gtcatataaa gaagagtatg aaaaaattaa
    15181 tatagatgat gaggatattg aatatctatc caatactctc gagcaaggaa tctcattgtt
    15241 atataatttt tctggtctaa atgaggaaca ggaacgaaca atgaaacaat tggttacatt
    15301 attgaataaa gatactttaa aaacaatgca gttgattgga tttaattata agcaagcaat
    15361 aggagaacca ctaaccgaag cttgtgcaac tgcaattaaa aataagttta taactcagaa
    15421 tagtaaaggc aaaaggaagt agtggtataa tccagttgta atatcacaaa tagcctcaaa
    15481 tgttatttca aaaatgatca atattaagaa atagaggaat tatgagaatc gcagataaaa
    15541 ccgttacccg cgctattttg gagcgtcatg ggtttacatt taagaaatca ttcggtcaga
    15601 actttttgac cgatactaat attttacaga agattgttga cacggcagag attgacaagg
    15661 gtgtcaacgt gattgagatt ggtcctggta tcggggcttt gacagagttt ttggctgaga
    15721 atgcagctga ggttatggct tttgagattg atgatcgtct cattcctatc cttgctgata
    15781 cgcttgcgcg ttttgacaat gtccaagtgg tcaatcagga tatcctcaag gctgatttgc
    15841 agacccagat tcaggctttc aagaatcctg atctgcctat caaggtggtc gcaaacctac
    15901 cttactacat cacaacacca atccttatgc acttgattga gagcaagatt ccttttgcgg
    15961 agtttgtggt catgatacaa aaagaagtgg cagatcgtat ttcagctatg ccaaatacta
    16021 aagcatacgg ttctttgtca atcgcagtgc agtattatat gactgccaaa gtatcattta
    16081 tcgttccgag aactgttttt gttcctgctc caaatgtaga ttcagctatt ttgaaaatgg
    16141 tctgtagaga tcaaccagta gtgagtgttc aagatgagga tttctttttc agagtgtcaa
    16201 aagtagcctt cgtacatcgt cgtaaaacac tttggaataa tctaacgagt cattttggaa
    16261 aatcagaaga tactaaagca aaattggaaa aagcattaga aatagcaaaa attaagccat
    16321 ctattcgcgg agaagcctta tctatccctg attttgcaag tttagcagat gccttgaaag
    16381 aggttgggat ataaactatg ttggttgaca agaagtggcg ttttgaagat agtgcaagtt
    16441 atttcgcttg tcctaaatgt caaaatccct tgataaaaga aagtaacagc ctcaaatgct
    16501 ctgataatca ttgttttgat ttatccaaat ttggatatgt taatcttctt ggaggaaaga
    16561 aggtagatga acattatgat aaaaaatcat ttgaaaatcg gcagcttgtc ttagaaaacg
    16621 ggtattataa tcatatttta gaagctattt caaaggtttt ggaaaataat tctcagtttc
    16681 attcggtttt agatattggt tgtggagagg gcttttattc tcgtcaatta gttaacaagc
    16741 atgagaagac tttcttggct tttgatattt ctaaagattc gattcaatta gctgctaaaa
    16801 gtgatcagtc taggcttgtg aaatggtttg tttctgattt agctaacctt cctattcaag
    16861 actcatcaat tgatatcatt ttagacattt tttctcctgc aaattataaa gaatttaggc
    16921 gggtgctgag tgatgatggt atccttgtaa aggttgttcc tgtagctgaa catgttcaag
    16981 aattgcgaga aaaagctagt caatacttga aacagaaaga ttactctaat caaaaaattc
    17041 tagatcactt tagagaaaat tttgaaatta tttctgagca gaaagttgtt caaagctata
    17101 actgttctca acaagaacgt caagctttta ttgatatgac accgttgtta ttcagcgtag
    17161 ataaaacgac tattgattgg gcgagtatat cagaaattac agtaggtgcc ttaatagtaa
    17221 ttggaaaaaa gcgcagtgta tctaaataag atcactgctt ttatgttggg aattcataaa
    17281 attgaggtta tttgagagaa atcgaacacc tgcaaatagt ttctatttag cttatctctg
    17341 agagtagagc ttatttctca ttttatgata taatagtggt attctaaaca attaaggaga
    17401 acaattgcaa ggtagaattg tcaagtcgct agcaggtttt tattatgtgg aatctgacgg
    17461 tgttgtttat cagacacgtg cgcgtggaaa ttttagaaaa aaaggtcaaa ttccctacgt
    17521 tggggattgg gttgaatttt cttcgcaaga tcaatcagaa ggatatattc tatcaattga
    17581 agagcgtaaa aatagcctcg tacgaccacc tattgttaat attgatcaag ctgtcgttat
    17641 catgtctgct aaagaacctg attttaacgc taacttatta gaccggttct tagtacttct
    17701 agaatataag atgattcagc ctatcattta tatttccaag ttggatttgt tagacgattt
    17761 ggttgttatt gatgatattc gcgaacttta tcagaatata ggctacgttt tctgttattc
    17821 tcaagaggaa ctcttgcctc tgttagcaaa taaagtgact gtttttatgg gccagacagg
    17881 tgtcggaaaa tcaacgcttc tcaacaaaat tgctcctgag ttgaaacttg aaactggtga
    17941 aatttcgggt agtttaggcc gtggccgaca caccacaaga gcagttagtt tctataacgt
    18001 tcataaagga aaaattgcag acactcctgg tttttcttct ttggactatg aagtggataa
    18061 tgcagaggac ttaaacgaat cctttccaga gcttcgtaga cttagtcatt tttgtaaatt
    18121 ccgctcgtgt acgcatacac atgagcctaa atgtgctgta aaagaagcct taactcaagg
    18181 tcagttatgg caagtccgct atgataacta cctccaattt ttaagtgaaa ttgagaaccg
    18241 tcgtgaaaca tataagaaag ttattaaaag aaagtaggtt tctatgtcaa ccaataaaat
    18301 tgccccatcg atattagcag cagactatgc taattttgct aacgaattga aacgtattga
    18361 agagacaaca gcagaatacg ttcatattga tatcatggat ggtcaatttg tccctaatat
    18421 tagttttggt gcggatgttg tatccagcat gcgtaaacac agtaaacttg tttttgattg
    18481 tcatctgatg gttgttgatc cagaacgtta tattgaggct tttgctcaag ctggtgctga
    18541 tatcatgacc attcatgttg aagcaacgaa acatatacat ggtgcgcttc aaaagataaa
    18601 agaggcagga atgaaagcag gtgtggttat taacccagga acacccgtag aatctcttat
    18661 tcctatctta gatttggttg accagatttt aattatgact gttaatcctg gctttggtgg
    18721 acaagccttt attccagaaa tgatgtcaaa agtaaagaca gttgcagcat ggcgcaaaga
    18781 gtacggtcat cattatgata ttgaagttga tggcggtata gataatacta caattaaggc
    18841 agcagcagag gctggtgcaa atgtctttgt tgcaggttct tatctcttta aagcaagtga
    18901 tttacctgca caagtagaaa ccttaagggt ggcgctcgat gactaaaatt gctttatttg
    18961 ctggcggtga cttaacttat tttgaatatg attttgacta ttttgtcgga attgaccgtg
    19021 gaagcctatt tttattaaaa aatgggcttt ctttggacat ggcagtgggt gattttgatt
    19081 ctataacaga agatgaactg ctttatatca aacactattg tagcaatatc gtttctgcta
    19141 gtgcagaaaa aaatgataca gatacagaat tagctttaaa aactattttc aaagaatttc
    19201 cagaagctca ggtgaccgta tttggagcat ttggtggcag gattgaccat atgatgtcta
    19261 atatcttttt accaagtgac agagatctag aaccatttat gtcacaaatc agactcaaag
    19321 atgaacaaaa catagtgact tatctaccat ccgggaaaaa tcaagtctca aggattgaag
    19381 ggatgtccta tgtctcgttc atgcctgaga gtgagtctac tttgcagatt tctggggcta
    19441 aatatgaatt gaacaaaagt aattatttta agaaaaaaat gtattccagt aatgaattta
    19501 tgacttcgcc aattgaagta gagctcaaag atggttactt aatcattatt tatagtaagg
    19561 atagaggtta atatgcttgt catcattttg atcattgtac tagctagtct gacagtgacg
    19621 ataatttctt acccaaaaat gacggaatta acaaagtccg ttgaaaaaca acttgaagat
    19681 aatgctgata atctatcaga ccaactgaca tatcagatag aagtggcgca aaaagatcaa
    19741 attctaacgt tgactaatca gctaaaccgt atgcaacagg aaatttatca gttgcttacc
    19801 gatatgcgta cagaattaaa tcagcattta acggagagtc gagatcgatc agacaaacgc
    19861 ttggaattga ttaactccaa tttatctcag tcagttcaga aaatgcaaga ttccaatgaa
    19921 aaacgcttgg atcaaatgcg ccaaactgtt gaggaaaagc tagaaaaaac gctacaaacg
    19981 cgtttgcaaa cttcttttga aactgtatcg cgtcaactag agagcgtcaa tcaaggtctt
    20041 ggggagatga aaactgttgc ccaagatgtt ggtacactga acaaagtctt gtcaaatact
    20101 aagacaaggg gaatattagg ggagttacaa ctcggccaaa ttatagaaga tattatgaca
    20161 gttagtcaat atgagagaga atttcctacg gtgtctggct cttctgagcg tgttgaatat
    20221 gctattaaat tacctggaaa tggtcaggga gattatatct atttgcctat tgactctaag
    20281 tttcctctag aagattatta ccgattggaa gatgcttatg aattgggtga caaggttcaa
    20341 atagaactct atcgtaaatc tttactggca agtattcgta aatttgcaaa agatataaac
    20401 aataagtact taaatcctcc tgaaacgaca aattttggta tcatgttctt accaactgaa
    20461 gggctctatt ctgaagtggt aagaaatgca acattctttg atagtctaag acgtgacgaa
    20521 aatattgtag tagctggacc gtcaacctta tctgctttac taaattcatt atctgttggc
    20581 tttaaaacct taaatattca aaagaatgct aatgatatta gtaaaatttt agggaatgtc
    20641 aaggttgaat ttggaaaatt tggtggcatg ttatcaaaag ctcaaaaaca attaaataca
    20701 gcaagtaaaa gtattgatag tttactcaca acgcggacaa acgctattat cagagtctta
    20761 aatacagttg aagaacatca agatcaagca accactagct tgttaaacct tcctataaca
    20821 gaagaagagg aaattaatga aaattaacca aatgaaaaaa gacgagcttt ttgaggggtt
    20881 ttaccttata aagaaagcag aagttcgtaa aacgcgtgca ggtaaagact ttattgcctt
    20941 tacctttcaa gatgatactg gcgaaatttc aggaaatatg tgggatgcac agacctataa
    21001 tgtagaagag tttgtagccg gtaaaattgt tcatatgaaa gggcgtcgtg aagtttataa
    21061 tggaactccc caagtcaacc aaattacatt aagaaacatc aaagatggtg aacctaatga
    21121 tcctagagat tttaaagaaa aacctccaat caatgttgac aacgtgagag agtacatgga
    21181 acaaatgctc tttaaaattg aaaatgcaac ctggcagcgt gtggtaagag cactttatcg
    21241 taaatacaat aaggaatttt ttacctatcc agccgccaaa acaaaccacc acgcttttga
    21301 atcaggattg gcatatcaca cggcaacaat ggttcgtttg gcagatagta tcggagatat
    21361 ctatccagaa cttaataaaa gtttgatgtt tgctggtatt atgctacatg atttagccaa
    21421 ggtcattgag ttatcgggtc ctgataatac agaatatact attcgaggta atcttatcgg
    21481 tcatatttca cttattgatg aggaattaac aaaaatacta gcagagctta atattgatga
    21541 tacaaaagaa gaggttacag tgttacgcca tgttattctg agtcatcatg gacaattaga
    21601 atatggaagc ccggttcgtc ctagaatcat ggaggcagaa attatccata tgatcgataa
    21661 cattgatgca aatatgatga tgatgacaac agctttaaac cgtgtcaacg aaggtgaaat
    21721 gacaaatcgc atttttgcca tggataaccg ttctttttat aaaccgaaca ttaaataatt
    21781 taggtagaat aattaagacc taaaataaaa tgttcgtttt tagcatttgt tatgttatac
    21841 tatatgtaaa gaatgctatg aataaggaga aaatatgaaa ttaagacgta gcgaacgcat
    21901 ggttgttatt tcaaattacc taataaataa tccctataca ttaactagtt taaatacatt
    21961 tgctagtaaa tacggtgccg cgaaatcttc gatttcagaa gatattgcta ttatcaaaaa
    22021 agcttttgaa caagctcaaa tcggagatat taaaactgtg actggtgcaa gtggaggtgt
    22081 tatctttaca ccaactattg ctgaagcaga agcgaaagaa atcgttgaag aattaagaca
    22141 gcgtttatct gaaaacgatc gtattcttcc gggaggttac atttatcttt cagatctact
    22201 cagtacacca aaaatgttac aaagtattgg tcgcattata gcgaacgctt acagaggtca
    22261 aaaaattgat gctgtcatga cggttgcaac aaaaggtgtt cctttagcaa acgcagtagc
    22321 aaatgtttta gatgttcctt ttgttattgt gagacgtgat ttgaagatta cggaaggttc
    22381 tactgtgtca gtaaactacg ctagtggttc aagtggccgt attgaaaaaa tgtttctttc
    22441 taagcgtagc ttaaaaccta acagtcgagt tttaatagtt gatgatttct tgaaaggtgg
    22501 aggtactgtt tcggggatga ttagtttact ctcagaattc gatagcacat tagtaggtgt
    22561 cgctgtattt gcagaaaatg ctcaggaaca acgtgagaag atggcctata aatctcttct
    22621 gagagtatct gaaattgatg taaaaaataa tagagtctct gtagaagcgg ggaatatatt
    22681 tgattaaaga gtcaaggtga catccttgat tctttttgct tgtaaaagta ataaaagctt
    22741 gacacattat gtgtaaaatg atactatgat aaacggtact tttacttttg gtctctcaaa
    22801 agtgtacaga gacgtgctga caattgttgc aaaagtacac gcagatatag gctgtcacca
    22861 agtgctatat caaccaaaaa taaaaaactt acaggagaaa tgtagatgcc tacaattaac
    22921 cagttggtac gtaaaccacg taaatctaaa gttgaaaaat cagattcacc tgctttgaat
    22981 attggttaca acagtcacag aaaagttcac actaaattat ctgcaccaca aaaacgtggt
    23041 gttgcaactc gtgttggaac tatgacacct aaaaaaccta actctgccct tcgtaaattt
    23101 gctcgtgtac gtttgagcaa ccttattgaa gttactgctt acattccagg tatcggacac
    23161 aacttgcaag aacacagtgt tgtgcttatc cgtggtggac gtgtaaaaga ccttccaggg
    23221 gtacgttacc atatcgttcg tggtgcactt gatactgcag gtgtagctga tcgtaaacaa
    23281 ggccgttcta aatacggtgc taaacgtcct aaagggtaat tagaaggggg ataagagaaa
    23341 atgagtcgta aaaatcaagc gcctaaacgc gaagtattgc cagatccatt gtataactca
    23401 aaaatcgtta cacgtcttat caaccgtgta atgcttgatg gtaaacgtgg tactgctgca
    23461 actatcgtat atgatgcttt tgaagcaatt aaagaagcta ctggaacaga tgctcttgaa
    23521 gtgttcgaaa cagctatgga aaacatcatg cctgtacttg aggtacgtgc acgccgtgtt
    23581 ggtggatcaa actaccaagt tcctgtcgaa gttcgtccag aacgtcgtac aactttagga
    23641 cttcgttggt tggttaacgc atcacgtgct cgtggtgaac acactatgaa agatcgtctt
    23701 gcaaaagaaa tcatggatgc tgcaaacaac acaggagcat cagttaagaa acgtgaagat
    23761 actcacaaaa tggctgaagc taaccgtgcc tttgcacact tccgttggta agattactct
    23821 ctttttgaga cttatcatag ctactaaaca aattgtttgg tagatagcaa tgttagcaat
    23881 tagaacgggt aggtcctgcc tatccgtttt ttataaatag tgttataata aagaagtaaa
    23941 taatttaaaa tataggagaa accaaatggc tcgcgaattt tcacttgaaa aaactcgtaa
    24001 tatcggtatc atggctcacg tcgatgctgg taaaacaaca acaactgagc gtattcttta
    24061 ctatactggt aaaatccaca aaatcggtga aacacacgaa ggtgcttcac aaatggactg
    24121 gatggaacaa gaacaagaac gtggtatcac aattacatca gctgcaacaa cagcacaatg
    24181 ggatggtcac cgtgttaaca tcatcgatac accaggacac gtggacttca caattgaagt
    24241 tcaacgttca cttcgcgtac ttgatggtgc ggtaacagtt cttgatgctc aatcaggtgt
    24301 agaacctcaa actgaaacag tttggcgtca agcaacagaa tacggagttc cacgtatcgt
    24361 ttttgctaat aaaatggata aaatcggtgc tgacttcctc tattcagtac aatcacttca
    24421 tgaccgtctt caagcgaatg cacacccaat tcaattgcca attggttcag aagacgattt
    24481 ccgtggtatt attgacttaa tcaaaatgaa agctgaaatc tatactaatg accttggtac
    24541 agatatcctt gaagaagata ttccagcaga gtatgttgat caagcaaatg aatatcgtga
    24601 aaaattagta gaagcagttg ctgatactga tgaagatttg atgatgaaat accttgaagg
    24661 tgaagaaatc actaacgaag agttgatggc tgctattcgt aaagcaacta tcaatgttga
    24721 attctaccct gtattgtgtg gttcagcctt caagaataaa ggtgttcaat tgatgcttga
    24781 tgctgttatt gattaccttc caagcccact tgatattcct gcaatcaaag gtattaaccc
    24841 tgatactgac gaagaagaaa cacgtcctgc ttcagatgaa gagccatttg cagcgcttgc
    24901 cttcaagatc atgacagatc ctttcgtggg tcgcttaaca ttcttccgtg tgtactcagg
    24961 tgttcttaat tcaggttcat acgtgttgaa tacatctaaa ggtaaacgtg aacgtatcgg
    25021 acgtatctta caaatgcacg caaatagtcg tcaagaaatt gaaactgttt atgctgggga
    25081 tatcgcagcg gctgttggtt tgaaagatac aactacaggt gattcattga ctgatgaaaa
    25141 atcaaaagtt attcttgaat caattgaagt tccagaacca gttatccaat tgatggttga
    25201 gccaaaatct aaagctgacc aagataaaat gggtatcgcc cttcaaaaac ttgctgaaga
    25261 agatccaacg ttccgcgttg aaactaacgt tgaaactggt gaaacagtta tctcaggaat
    25321 gggtgaactt caccttgatg tccttgttga ccgtatgaaa cgtgaattca aagttgaagc
    25381 aaacgttggt gcacctcaag tatcataccg cgaaacattc cgcgcttcta cacaagcacg
    25441 tggattcttc aaacgccaat ctggtggtaa aggtcaattt ggtgatgttt ggattgaatt
    25501 tacaccaaac gaagaaggta aaggattcga attcgaaaat gctatcgtcg gtggtgtggt
    25561 tcctcgtgaa tttattccag ccgttgaaaa aggacttgta gaatctatgg caaacggtgt
    25621 tcttgctgga tatccaatgg ttgacgtaaa agctaaactt tacgatggtt cataccatga
    25681 tgttgactca tctgagacag ccttcaagat tgctgcatca cttgcactta aagaagctgc
    25741 taaatcagcg caaccagcta tccttgagcc aatgatgctt gttacaatta cagctccaga
    25801 agacaatctt ggtgacgtta tgggtcacgt gactgcacgt cgcggtcgtg ttgatggtat
    25861 ggaagcacgt ggtaatacac aagttgttcg tgcctttgtt ccacttgctg aaatgtttgg
    25921 ttatgcaaca gttcttcgtt ctgcaacaca aggacgtggc acattcatga tggtatttga
    25981 ccactacgaa gatgttccta agtcagttca agaagaaatc atcaagaaaa actctggtga
    26041 ataatttctg aaaacttatg aaaggtactc gaaagagtat ctttttttct ttttgaaagt
    26101 ggtaaattca ttgcaatttt tctcgaaaaa agatataata attgtgttga aagatttttg
    26161 cacatatgta caagttaatc ttttcacatt gattaggggg atccctaaaa taaaatattt
    26221 gagattttca taaggaggaa atcactaatg gtagttaaag ttggtattaa cggtttcggt
    26281 cgtatcggtc gtcttgcatt ccgtcgcatc caaaacgtag aaggtgttga agttactcgt
    26341 atcaacgacc ttacagatcc aaacatgctt gcacacttgt tgaaatatga cacaactcaa
    26401 ggtcgtttcg acggtactgt tgaagttaaa gaaggtggat tcgaagttaa cggtcaattt
    26461 gttaaagttt ctgctgaacg cgaaccagca aacattgact gggctactga tggcgtagaa
    26521 atcgttcttg aagcaactgg tttctttgca tcaaaagaaa aagctgaaca acacatccat
    26581 gaaaatggtg ctaaaaaagt tgttatcaca gctcctggtg gaaacgacgt taaaacagtt
    26641 gttttcaaca ctaaccacga tatccttgat ggaactgaaa cagttatctc aggtgcttca
    26701 tgtactacaa actgtcttgc tccaatggct aaagctttac aagacaactt tggtgttaaa
    26761 caaggtttga tgactactat ccacgcatac actggtgacc aaatgatcct tgacggacca
    26821 caccgtggtg gtgaccttcg tcgtgctcgt gcaggtgctg caaacatcgt tcctaactca
    26881 actggtgctg caaaagctat cggacttgtt atcccagaat tgaacggtaa acttgatggt
    26941 gctgcacaac gtgttcctgt tccaactgga tcagtaactg aattggttgc aactcttgaa
    27001 aaagacgtaa ctgtcgaaga agtaaatgca gctatgaaag cagcagctaa cgattcatac
    27061 ggttatactg aagatccaat cgtatcatct gatatcgttg gtatttcata cggttcattg
    27121 tttgatgcta ctcaaactaa agttcaaact gttgacggta accaattggt taaagttgtt
    27181 tcatggtacg ataacgaaat gtcatacact tcacaacttg ttcgtacact tgaatacttt
    27241 gcaaaaattg caaaataatt tttaattaat atgatgtaaa agagggattt atccctcttt
    27301 tatttttata aataaaaaat aatacgaatg ttttttcatt ttattataga aaattatcag
    27361 aaaataaatg aaaaatgaat tatattgact ataagttaaa ataaggtttg attttgaaag
    27421 taagcagtgg tatgatttaa ttgaaaaaat atagaggaga agttttatga agactacaaa
    27481 agttttttca gttttatcgt tatctgttgc tgtatttatg atg
//
LOCUS       DATRME010000018        27085 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRME010000018 DATRME010000000
VERSION     DATRME010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27085
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            238..1260
                     /locus_tag="VBC23_000808"
     CDS             238..1260
                     /locus_tag="VBC23_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6842774.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            1631..2923
                     /locus_tag="VBC23_000809"
     CDS             1631..2923
                     /locus_tag="VBC23_000809"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6842775.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(2946..3239)
                     /locus_tag="VBC23_000810"
     CDS             complement(2946..3239)
                     /locus_tag="VBC23_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842776.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
     gene            3578..4195
                     /locus_tag="VBC23_000811"
     CDS             3578..4195
                     /locus_tag="VBC23_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842777.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            4363..5802
                     /locus_tag="VBC23_000812"
     CDS             4363..5802
                     /locus_tag="VBC23_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6842778.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            5830..6108
                     /locus_tag="VBC23_000813"
     CDS             5830..6108
                     /locus_tag="VBC23_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6842779.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            6175..6660
                     /locus_tag="VBC23_000814"
     CDS             6175..6660
                     /locus_tag="VBC23_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6842780.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            6773..7438
                     /locus_tag="VBC23_000815"
     CDS             6773..7438
                     /locus_tag="VBC23_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6842781.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            7442..8305
                     /locus_tag="VBC23_000816"
     CDS             7442..8305
                     /locus_tag="VBC23_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6842782.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            8307..9023
                     /locus_tag="VBC23_000817"
     CDS             8307..9023
                     /locus_tag="VBC23_000817"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6842783.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            9034..9681
                     /gene="fsa"
                     /locus_tag="VBC23_000818"
     CDS             9034..9681
                     /gene="fsa"
                     /locus_tag="VBC23_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6842784.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            9844..10827
                     /locus_tag="VBC23_000819"
     CDS             9844..10827
                     /locus_tag="VBC23_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6842785.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            11062..12081
                     /locus_tag="VBC23_000820"
     CDS             11062..12081
                     /locus_tag="VBC23_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842786.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINRDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            12100..13056
                     /locus_tag="VBC23_000821"
     CDS             12100..13056
                     /locus_tag="VBC23_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6842787.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            13154..14605
                     /locus_tag="VBC23_000822"
     CDS             13154..14605
                     /locus_tag="VBC23_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6842788.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            14627..15616
                     /locus_tag="VBC23_000823"
     CDS             14627..15616
                     /locus_tag="VBC23_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6842789.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            15682..17199
                     /locus_tag="VBC23_000824"
     CDS             15682..17199
                     /locus_tag="VBC23_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6842790.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIKTVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            17219..17980
                     /locus_tag="VBC23_000825"
     CDS             17219..17980
                     /locus_tag="VBC23_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6842791.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            18046..19725
                     /locus_tag="VBC23_000826"
     CDS             18046..19725
                     /locus_tag="VBC23_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6842792.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            20042..21133
                     /gene="ulaG"
                     /locus_tag="VBC23_000827"
     CDS             20042..21133
                     /gene="ulaG"
                     /locus_tag="VBC23_000827"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6842793.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            21223..23601
                     /locus_tag="VBC23_000828"
     CDS             21223..23601
                     /locus_tag="VBC23_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6842794.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            23978..25201
                     /locus_tag="VBC23_000829"
     CDS             23978..25201
                     /locus_tag="VBC23_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6842795.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            25220..26947
                     /locus_tag="VBC23_000830"
     CDS             25220..26947
                     /locus_tag="VBC23_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6842796.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         8513 a         4424 c         5439 g         8709 t
ORIGIN      
        1 taaaaaatta ttattgacta tggttatgct tttaggtaaa atgtgattat attagcaact
       61 aaggagtaat ccatgagata agcctaataa ttatgtcgga cttctttgtt gcaccttatc
      121 cagttcgtag tacaattaag tttcagtatt acctaaaggc gcctttattg aaattgaagc
      181 agtagtacac agacactgag aataaaaaac ttaactattt ttagcgggag tatatttatg
      241 aatatcatta ttgggaccag tcttttaata cttgtattag cgattttcac tttatttaat
      301 tataaagcac cctacggaac aaaagcaatg ggagcattgg catctgcggc ttgtgcatcc
      361 ttccttgtag aagcttttca agattctttc tttggtaaag ttttgggatt tcaattttta
      421 agtgaagtgg gtggtgctaa tggttcactt tctggtgtag ctgcagctat attggttact
      481 attgctattg gtgtaacccc aggatacgca gttttaattg gtttatctgt ttctggaaca
      541 ggtattattc ctggtttcct tgcaggatat ttagtgggat ttcttgttaa gtggatggaa
      601 agaaatattc ctggtggttt agatcttatt tcaattatta ttattggtgc accattaaca
      661 cgtcttgtgg ccaaactgtt gacgccgctt attaatagca ctttgctcac cattggagat
      721 atcttaacat caggtgctca tagcaaccct attttaatgg gggttatact tggcgggaca
      781 attgtagtag tggcgacagc accactttct tctatggcat tgacagctat gctaggatta
      841 accggaatgc ctatggctat aggagccttg tctgtctttg gttcgtcatt tatgaatggt
      901 gtacttttcc ataaattaaa acttggaagt cgtaaagata atatagcttt tgctgttgag
      961 cctctaactc aagctgacgt gacttcagct aaccctattc caatctatgt cactaatttt
     1021 gttggtggtg cagcttgtgg tattttaatt gccttgatga aattagttaa tgatactcct
     1081 ggaacagcga caccaattgc aggatttgct gtcatgtttg cctataaccc aatgataaaa
     1141 gtactaataa ccgctctagg ttgtattatc ctatctttac tagcaggcta ttttggaggc
     1201 attgttttta aagattataa attagttaca aaagaagagc tacaggctag agataactag
     1261 ttacttcatg aaaagctaat agtaaatatc attaatatag atatgatatc tgacttgtga
     1321 taactgttat tgataattta aaattcaacg actctaataa aaagattgtt gaattttttt
     1381 attgttaaga atgctggtat cagtaaattt caagatttct ttatcaacta taatgttagt
     1441 tttttaccgt ttttttacac ttctaataat tatttttttc tttttttccg ataatatttg
     1501 aggaatttgg caaaaaaaaa cattttttgc tataataagg tgtcagacaa actcgcaggt
     1561 gaaattcctg ccatgttttt ccagaaagga ttggggagtc ccaaaaaggc tcactcagtg
     1621 ttaaataaat atgacttcag ttgttgtagt aggtacccaa tggggtgacg aaggtaaagg
     1681 taaaattaca gattttttat ctgctgacgc agaagtaatt gcacgttatc aaggtggtga
     1741 taacgctggg catactatcg ttattgataa caaaaaattt aaacttcatt taattccttc
     1801 aggaattttc ttcaaagaaa aaatttctgt cattggtaat ggtgttgtcg ttaatccgaa
     1861 atcattagtt aaagagttag cttatcttca cggagaaggt gtaacaacgg ataaccttcg
     1921 tatttcagat cgtgcgcatg ttatcttgcc ataccacatc aagcttgacc aactacaaga
     1981 agatgctaaa ggtgataata aaattggaac aaccatcaaa ggaattgggc cagcttacat
     2041 ggacaaggct gcgcgtgtcg gtatccgtat tgctgacctt cttgatagag aagtttttgc
     2101 tgagcgtttg aaaattaatt tagctgagaa aaatcgttta tttgaaaaaa tgtatgacag
     2161 tacacctctt gaatttgatg atattttcga agaatattat gaatatggtc aacaaattaa
     2221 gcaatatgtg actgatacat ccgttatctt aaatgatgct cttgatgccg gtaaacgtgt
     2281 tctttttgaa ggagcacaag gagttatgct cgacattgat caaggaacat acccatttgt
     2341 aacatcttcc aatccagtag caggtggtgt cacaattggt tcgggagttg gaccaagtaa
     2401 aattaataaa gtggtaggtg tatgtaaagc ttacactagc cgtgttggcg atggaccatt
     2461 cccaacagaa ctttttgatg aggttggtga ccgtattcgt gagattggta aagagtatgg
     2521 tacaacgact ggtcgtcctc gtcgcgttgg atggtttgat tctgttgtta tgcgtcacag
     2581 ccgtcgagta tcaggtatta ctaacctctc tctgaattca attgatgttc tttcagggct
     2641 tgatacggtg aaaatttgtg tggcttatga ccttgatggg aaacgtattg actattaccc
     2701 agcaagcctt gaacagctaa aacgttgtaa accaatctat gaagaattac cgggctggtc
     2761 tgaagatatt acagcttgcc gtagcttaga tgatcttcca gaaaatgcac gtaattacgt
     2821 tcgccgtgtt ggcgaattgg ttggtgttcg tatttctact ttctcagtag ggcctggtcg
     2881 tgaacaaaca aacattcttg agtctgtttg gtcaaatatt taatattaaa aatctgactg
     2941 tacagtcaga ctttttgatg tttacttttt ctttttaata gatagatact aataagactt
     3001 aaggacttcc cagaaatcaa taaaactact ataattgata aaaaaagaag tgaattgata
     3061 ttgtgaatca aagtatgtaa taatactagc gatacaactg ttagaatact accagtcatc
     3121 aataagcgtt ccagaggata gtagccatgt tgcttatgca tttctaattt taatagtaga
     3181 ccagctgaca attctgctag gaagcaaata ggtagagcta tcaaattaat aagaatcata
     3241 gaccaaccaa atccttgacc ataacctata tatgatagac tatttttggg aattgttgac
     3301 gtgtgaatta taaaatcaat cgtaaagata atgtctataa gaagtagtag cgctaacaga
     3361 taatgtagta agaagattgt tcttaaccgt ttattaatca atatttcatt tcctcctact
     3421 tatttaccaa taagcttatt atatcataat gtataatcaa acattcgtta gtactgatta
     3481 aaaggtttat ggaggggcgg ttactattgt ttcattgaat tagtaacttt aacgacgaaa
     3541 tgttataatg ttattagttt ataaaagagg agattgtatg cgtaaatttt tccaagatgt
     3601 tattgtagag gtggcttatt atttagagat tgctttgtca gttattctgg caacagctat
     3661 tattttcttt agtttagcac tctttcatga tttgtttagc atgttgtctg ttgatcaaaa
     3721 tgttgataag ttgttccaga ctttccttgc tcgcgccatg acaatagcag tcgggattga
     3781 gttgatcaaa atgttttcaa aaccttcccc tgatacttta attgaagtgt tattgtttgc
     3841 cttagctcgt caacttgtaa ttgaacatgg tagtgtttca gattacctta tggggattgc
     3901 ttcagttgct attttgtttg ctgttcgtaa atatcttttc acccattttg atgagtcaag
     3961 caacattgtt ttacgtgcta ataacaaagt caaaaaagct aatattttag ctagggttca
     4021 actaccttgt caacccaatg aaactttagg agacttactt gttcgtcatt taaatgatga
     4081 ggaaaaggac gttgctattg gttcagtagt ctatattaat aacgttgcat tgcgtgttga
     4141 tagtatgcac gatggtcttg tgacgcgtgt agaaatcatt aggagtctac attaaaaaat
     4201 ataaaaaatc atgtatctta ggatacatga tttttttgta aattaggttc agcacaattt
     4261 tgcacactta agtgtgtact tcttggtctt ttttaaaacg cttacaaaaa atataataaa
     4321 gatacaaaaa aacattactt aaataaagag aggtctttag ttatggaaaa cttcctagct
     4381 ccccttaatt ggttctcgca aaatatcttg cagaatccag ccttcttcgt aggtctgttg
     4441 gtactcattg ggtatctttt attgaagaaa ccacttcatg atgtctttgc tggttttatc
     4501 aaagcgaccg ttggatattt gattttgaac gtaggtgcag gaggattggt taatacattc
     4561 cgtccaatct tagttgctct tgctaaaaaa tttcatttag aagctgctgt tattgaccct
     4621 tactttggtt tagcatcagc aaatgctaag ttagaaacaa tgggctttat cagtgttgca
     4681 acaacagcac tcttgattgg ttttgggatt aacattctct tggttgctct tcgtaaagta
     4741 actaaagtcc gtactctttt tattactggt catatcatgg tacaacaagc tgcaacaatt
     4801 tcagtatttg tactgctctt gattccacaa cttcgtaatg gttttggtgc atgggctgtt
     4861 ggtatcattt gtggtctata ttgggcggta agttctaata tgaccgttga agcaactcaa
     4921 cgccttacag gtggtggcgg atttgccatc ggacatcaac aacaatttgc aatttggttt
     4981 gttgataaag ttgcgccatt tttcggtaaa aaagaagaaa atcttgataa cttaaaatta
     5041 ccaacattct taaatatttt ccatgataca gttgttgcat cagcaacatt gatgctagtt
     5101 ttctttggag gaatcttagc tgtccttggt ccagacatta tgtctaatgt taaattaatc
     5161 ggacctggtg catttgtacc aactaagcaa gctttcttca tgtacatcct tcaaacatca
     5221 ttgacattct cagtttacct tttcattttg atgcaaggtg tacgcatgtt cgttacagaa
     5281 ttgacaaatg ccttccaagg tatctcaaac aaacttcttc caggatcatt cccagcggtt
     5341 gacgttgcag cttcatatgg ttttggttct tcaaatgcag ttctttcagg atttgctttt
     5401 ggtttgattg gtcaattaat tacaatcgct ctacttgttg tcttcaaaaa tccaatcttg
     5461 attatcacag gattcgttcc agtattcttt gataatgcgg caatcgctgt ttatgcagat
     5521 aaacgtggtg gctggaaagc agcagtagct ctatcattta tctcaggtat tattcaggtg
     5581 gcacttggtg ctgttgctgt aggtctttta ggacttgcag gtggttatca tggaaatatc
     5641 gactttgaat tcccatggtt agcatttggt tacatcttca agtatttagg tattgctggt
     5701 tacgtgattg tatgtctctt cttcttagct attccacaac ttcaatttat gaaatctaaa
     5761 gataaagaag catactatcg tggtgatgcg cctgcagaat aatagaaatt tataacggag
     5821 gaaataaaaa tggttaaagt tcttacagca tgcggaaacg gcatgggatc atcaatggtt
     5881 atcaaaatga aagttgaaaa tgcgcttcgt caattaggcg ttagcaactt tgagtcagca
     5941 tcttgctcag tcggagaagc aaaaggttta gctgcgaact atgatatcgt ggttgcttca
     6001 aaccacctta tccatgaatt agacggacgt acaaaaggtc atttagtagg tcttgacaac
     6061 cttatggatg acaatgaaat aaaaacaaaa ctacaagaaa ttttgtaagt taatagacaa
     6121 agggagtgag acaaaagttt ctctcccata tctttagact tttaaggaga aatcatgaat
     6181 ttaaaacaag cctttatcga aaatgactca attcgtctta aattatctgc aagcgattgg
     6241 aaagaagcaa tcaaattatc aattgatcca ttaattgaaa gtggtgctgt tgatgcagaa
     6301 tattacgatg ccattattga atctacagag gaatttggtc cgtactacat tcttatgccg
     6361 ggtatggcga tgcctcatgc tcgtccagaa gcaggcgtaa aacgtgatgc attttcatta
     6421 attacattaa cagagccagt agtctttcca gatggcaaag aagtctcagt attgttagca
     6481 cttgctgcaa caagttcagc tattcatact tctgttgcta tccctcaaat tattgccctt
     6541 tttgaattgg aaaattcaat tcagcgcttg acggaatgcc aagaagcaaa agaagtcctt
     6601 gcaatggtag aggaatcaaa aaacagccca tatttagaag gtttagattt agaaagctaa
     6661 tcaataaact gatattaata gaaaggatag gacttataaa aaatctctgt gataagaaac
     6721 tctttgccac aaataaagtg agtattttta tcaggtcctt agaataaaca acatgacaaa
     6781 acgtttacca aatttacaag ttgcacttga ccactcagat ttacaaggag ctatcaaagc
     6841 tgccgtatca gtaggacatg aagttgatgt tattgaagct ggaactgttt gccttcttca
     6901 agtaggtagt gaattagttg aggtccttcg cagtctcttc cctgacaaaa taattgttgc
     6961 tgatacaaaa tgtgctgatg ctggtgggac tgttgctaaa aataatgctg ttcgcggtgc
     7021 agactggatg acttgtatct gttgtgctac tattccaaca atggaagcag ccttaaaagc
     7081 tatcaaagaa gaacgcggcg atcgtggaga aattcaaatc gaactatatg gtgattggac
     7141 ttatgagcaa gcgcaacaat ggttggatgc aggtatttca caagccattt atcaccaatc
     7201 tcgtgatgcg cttcttgctg gcgaaacatg gggagaaaaa gatcttaata aggttaaaaa
     7261 attgattgat atgggcttcc gagtttctgt aactggtggt ttatcaactg atactcttca
     7321 attatttgaa ggtgttgatg tctttacttt tattgcgggt cgtggtatta cagaggcaga
     7381 tgatccagcc gcagcagcac gtgcatttaa ggatgaaatt aaacgtattt gggggtaatg
     7441 tatgacacgt ccaataggaa tttacgagaa ggcaacacca aaacatttta actggttaga
     7501 acgcctacaa tttgctaaag aattgggatt tgattttgta gaattatcca ttgatgagtc
     7561 agatgaacgt ttggcacgtt tggagtggtc aaaagaagaa cgccttgagt tggtcaaagc
     7621 tatttttgag acaggagttc gtgttccaac aattacattt agtggtcatc gtcgtttccc
     7681 aatgggatct aatgatcctg agaaagaagc gcgtgcgatg gatatgatga aaaaatgtat
     7741 cgtctttgct caagatattg gtattcgtaa tattcaatta gcaggatatg atgtgtacta
     7801 tgaagaaaaa tctccagaga cacgtgcacg ctttattaag aatcttcgtc aagcttgtac
     7861 ttgggccgaa gaagcacaag ttattctgtc tattgagatt atggatgacc catttatgaa
     7921 ttccattgag aaatatttgg ctgtggaaaa agagattgat tctccttatc tctttgtcta
     7981 cccagatact ggaaatgtat cagcatggca taatgatctt tggagtgaat tttataatgg
     8041 acatcgctca attgcagcgc ttcatattaa agatacctat gctgttaccg aaacgtctaa
     8101 aggtcaattc cgtgatgttc catttggtca agggtgtgtt gattgggaag aaatgtttgc
     8161 tgtcattaag aaaacaaatt ataatggtcc attcttgatt gaaatgtggt cggagaactg
     8221 tgagactgta gaggaaacac gtgcagccat taaagaggcg caagattttc tttacccatt
     8281 aatggagaaa gcaggagtga aataatatgg caaaatcatt gcaagaaatg cgtgaacgcg
     8341 tgtgtgaagc taataaatca ctaccagttc atggtttagt taaatttact tggggtaacg
     8401 tttctgaagt tgaccgcgaa gcaggattga ttgttatcaa accttcagga gttgactatg
     8461 accaattgac acctgaaaat atggttgtca cagacttaga gggaaatata gtggaagggg
     8521 atttgaatcc atcatctgat cttccaactc atgttcaact ttataaagca tggccagaag
     8581 tcggtggtat tgttcatact cattcaacag aagcagttgg ctgggcacaa gcgggtcgcg
     8641 atattccatt ttatggaaca acacatgcag attattttta tgggccagta ccatgtgcta
     8701 ggtcacttag tgaagatgaa gttaacactg cttatgaaaa agaaacaggt agtgttatta
     8761 tagaggaatt tgaacgtcgg aatttagatc caatggctgt tcctggtatt gttgtacgta
     8821 atcatggacc atttacttgg gggaaagatc cagcacaagc agtttatcac tcagtcgttc
     8881 ttgaagaagt tgccaagatg aatcgtttca ctgaacaaat taatccacgt gttgaaccag
     8941 ctccaaaata tattatggat aaacactatt tacgtaagca tggaccaaat gcttattatg
     9001 gtcaaaaagg acaagaacat taaggagaat aatatgaaat actttttaga tactgccgat
     9061 gtttctgaaa ttcgtcgtct taatcgttta ggaatcgttg atggtgttac taccaatcct
     9121 actattattt cacgtgaggg gcgtgacttt aaagaagtta ttaacgaaat ttgccaaatc
     9181 gttgatggtc cagttagtgc agaagtgaca ggtttaactt gtgatgaaat ggttaccgaa
     9241 gctagagaaa tcgctaagtg gtcaccaaat gttgttgtta aaattccaat gactgaagaa
     9301 ggtttagcag cagtttcaca attatcaaaa gaaggtataa agacaaatgt gacattaata
     9361 tttactgtgg ctcaagggct ttcggcaatg aaagctggtg caacctttat tagtcctttt
     9421 gtgggtcgtc ttgaagatat tggaacagat gcttatgctt tgattcgtga ccttcgtcac
     9481 attattgatt tttatggttt ccaatctgag attattgctg ctagcattcg tggattggcg
     9541 catgtggagg gcgtcgctaa atgcggtgcg cacattgcca ctattccaga taaaacattt
     9601 gcatcactat ttactcaccc gcttactgat aaagggattg aaactttctt aaaagattgg
     9661 gatagtttca agaagaagta gtgttacgat aaagcaaact cttaattata ctagtgaaag
     9721 acaaactttg ataatgggtt tgtctttttt ataatcttct ccaataagaa cacttatttt
     9781 catttttctt attaaatgat ataatacgga taaattaagt ctaattagaa taaggaaata
     9841 gagatgtttg agaaggtaac cattagtgat attgcaaatt tagtaggtgt gtcaaaagct
     9901 acagtttcct attatctcaa tgggaactat aagaaaatgt ctctgcaaac gaaagaaaaa
     9961 atccgtttag ctattaagga gactggttat caaccaagta agattgctca aagtttagtt
    10021 acaaaaaata cccgtactat tggggtcgtt attgctgata ttaccaatcc atttatttcg
    10081 tctgtgatga agggaataca tgatacttgt caacaatttg gttactcggt taactttact
    10141 aactcggata atgatattga cattgagttg gaaaatttaa accgtttaaa tcagcaaaac
    10201 gtttcaggaa ttattttaga ttctgtagat cctaatcatt catttataga aactttgagt
    10261 aatgatcgtc ttgtcatggt tgatagacaa gctaaagata ttaaggtaga tactgtagta
    10321 tccgacaata aagaatcgac tcaaatattt cttgaaaaaa tgcaagaaac tgggtatcat
    10381 gacatctatt ttgtcactta tcccattgaa gggatttcaa cccgtgagct tcgttacgaa
    10441 ggttttaaag aagtggtcag ttcaaatcct gataagttga ttattatcac tgaagatggt
    10501 agcacacaga gaattcttga tattattgaa cattcagagc aaaaaccagg gttcttaatg
    10561 atgaacggtc caactctttt gaattttatg aaaaaattaa atcaatcaac ggtcagctac
    10621 cctgaggatt atggtcttgg gagttacgaa gatttagaat ggatgcaggt gcttacacca
    10681 aacgtttcct gtataaaaca ggattcatat ggcattggtt gtctggctgc ccaatgttta
    10741 attgaaaaaa ttagtcaagg aaacgaacca actactgcaa ggttactaga agtaaaaaat
    10801 caaatcgtta ttcgtcaatc tttttaaaaa tgtttgcgag agcaagcatt tttttataaa
    10861 aaagcaaaaa acactatgta aacgcttgct tttataaaaa atgatagtat aatagaacta
    10921 taaatgagtt aaccgtttaa cttaagaata ggtaagcttt taacgaacca tctctaactc
    10981 tcttaactca aatgttttta tttttttgca ccattagtta aacggttaac tcaagtttta
    11041 tcttcaccaa aggaggatag aatgaagatt aataaattat tgtcagacag acttgaagct
    11101 tgttattcag tttgtcgttt gcaaaatcaa gactatgatt ttttattagt agcttcagag
    11161 gttgatcaag cctgctttgc ttatgatttg aatagcgatt tagcaaaaat tgttgtttgg
    11221 gaagatgtgg gaggtaccat gagtattatt ccaattcccg gaaccatgga ttttttggca
    11281 acccagaaat tttacccagg gtttaaggcc aaagattgtc aggttgttta tgcaagtttt
    11341 caaggagatc attgggacat ttgtaacgca tttgattttc cgtatttaca tcgctttgat
    11401 ctcattaaag gtaaagaaaa tcaactttac tttataggtt gtacaattgc taacagtaaa
    11461 gcctacactg aggattggag tgataaaggc cgaatttttg ttgctcgttt taatactcaa
    11521 aaccatacat tggaaggatt gcaacaattg cctcaaactt tattaaaaaa tcatggatac
    11581 tatgccattc aggatgaagg atattcattg attacttcag tagaaggggt actcaaactc
    11641 acttatccag aattttctac tacaggcgac tggcaattag aacggctttt cgatgaggag
    11701 acaagcgatg tggtgaaagt ggatattaat cgggatggta aggatgagta tgtgatcatc
    11761 caaggttttc atggagatcg tttacgtatc ttcactgaag atttcggtcg agaattattc
    11821 cattatcctg aaaaaacccc atttggtcac gctatttgga gtggtcgttt acttaatcag
    11881 acttgtttcg tattcgggtg gcgatcagaa aaagcagaat taaggctttt tcactttgta
    11941 gatgggcact tggtttcaga attagtagat gcaaaagcag cttctagtaa tgtcttagct
    12001 tttgaaaaag atggaaaagc ttatcttttc tcagccaata acggacgtgg cgaagttgct
    12061 ctttatcaat tagtaaaata aatttaagaa ggagacaata tggacaagaa aaaaatctta
    12121 gtaacgggta ttgtgcctaa agaaggtcta agaaagctta tggaccgatt tgatgttact
    12181 tattcagaag atcgcccatt ttcacgtgac tatgtgttag agcatttatc tgaatatgac
    12241 ggatggttac tcatgggaca aaaaggtgat aaagagatga ttgatgcagg tgaaaactta
    12301 caaattattt ctttaaatgc ggttggtttt gatcatgtag atactgcgta tgctaaagaa
    12361 aaaggaattg tagtctctaa ttcccctcaa gcagtacgag ttccgacagc ggaaatgact
    12421 tttgctttaa ttttagctgc cagtaagcgc cttgcatttt atgatagtat tgtaagaagt
    12481 ggagagtgga ttgatccgag cgaacaacgt tatcaaggtt tgactttgca aggttcaaca
    12541 ttaggtattt acggaatggg acgcatcggt ttgacagtcg caaattttgc taaagcattt
    12601 gggatgactg ttgtttacaa tgatgtttat cgacttcctg aagataagga aaaagagctt
    12661 ggtgtaacct atctcgaatt tgatcagctt attgaaacag ctgatgtcat aaccatccac
    12721 gccccagcat taccatcaac aatccacaaa ttcaataaag atgtttttgc taagatgaaa
    12781 aatcgaagct acttaatcaa cgctgctaga ggcccaatcg tttctgaaga agcacttatt
    12841 gaagctctga aagaaggtga aatcgcgggt gcgggattag atgtttttga gaatgaacca
    12901 caggtatctg aaggcttgag aagtcttgat aatgttatca tgagtccaca tgctgggaca
    12961 ggtactattg agggacgtag aactttggct gaagaagcag ctgacaacat cattgccttc
    13021 tttgatggca agccacaaaa tattgtcaat aagtaattgg ctagtgagtg cagagccatc
    13081 ttttgaaaaa atgggacctc ccattttttt acgggtaaat gtaagcgctt aataatttaa
    13141 taaaggtgaa cttatgctag aagctttaca taaattcttt gaagtttttg gtgcagcaac
    13201 tgttgtaccc gctatgattt ttattgtcag cctttttcta aaggtcaatc caaggcgagc
    13261 tttctttagc gccttacgag ctggtgttgg actcactggc tttggttgga ttatatcagc
    13321 ttttgctcct atggtgacta agtatatcca gcagatggtt aaaactactg gtctccattt
    13381 accaattgta gatattggtt ggcaggctgg ttctctcacc gccttttctt ctgagattgg
    13441 cctctctttc tttgtttttg gcttgctaat tgaattgggg cttttcttgc ttgggattac
    13501 ccgagtattt gtcccatcta atctttggaa taactttggc tatatgattt ggggtacaat
    13561 ggcctatgca gcaacaggta attttatatt gtctttcgca tttatggttt ttgtcttact
    13621 ctattcatta gtgatgtcag aagtattggc agatcgatgg tcggagtatt acggtgttaa
    13681 aaatgcaacg atcaactcga ttcataatat tgaaaccctt attccagctc ttattcttga
    13741 tcctctttgg aacctacttg gtgtcaataa ggtaaagttg aatccagaat cattaaagac
    13801 taagttaggg atatttggtg agccaatgac acttggtttt atacttggtg tgattattgg
    13861 tgtattaggt agtttacgta accttgcaag tattgatact tggggaggta tacttggttt
    13921 tgcagttgct cttgcagcgg ttatgactat ttttcctttg attacggggg tatttgctag
    13981 tgcttttgct cctcttgctg aagcagttga acgcaataag aaaaaagaaa gtcaagctga
    14041 gcaaggagca ctggataaaa aacgttggtt tattgcagta gatgacggtg ttggttttgg
    14101 agaaccagca actattatcg caggtctcat tttagtacca ataatggttg ttatttcctt
    14161 gattttacct ggaaatgaag ctttaccagt cgttgacctt attgctattc cttttatgat
    14221 tgaagctatg atagctgtgt caaaaggaaa tatccttaaa gctattttga atggaatcat
    14281 atggttttca ctaggtcttt atgctgctag tgccctaggt ccaatctaca cagaagctgt
    14341 taagcatttt ggaactgctt taccagcagg ggtaactttg attatgagct ttaatctcct
    14401 agctcatcct ttgacagctc tagtcttctt tgcttggata tctgggaatc cactctatat
    14461 tggtgtgaca gttattatct atcttgcttt cttgttcttg cttcgtactc gtcgtgaatc
    14521 tatttacgca taccttaaaa ctatggctga taagaatagc ggatatgaag gtagttgtga
    14581 aaaaatcttt attgctgaag attaaaaatc tgttggagga atactcatga aaaaaattga
    14641 tggacatgct catattgttg atacaattgc aggatttaat ggtaaagggc gtcttaatgc
    14701 ccttggaaac ggttatgcta tttgggatga tggtgattta attaagctca tcccagacga
    14761 atacggtgac aaagcattta cagctgaatc ttttttgaag tatatggatg ctaatggtgt
    14821 agataaagca gtcattttgc aaggtcatct taatggttat caaaattatt atacgcattt
    14881 agctattaaa cgttatccag aacgttttac aggcgcattc tctgttgatc catttgcaga
    14941 taatgcaatg caaattgtaa aacgtcatgt tgaagtcctt ggttttcgag ctattaaatt
    15001 tgaaattagt caaggagggg gtatccatgg ataccgtgga caaaaaacgc ctttccgtct
    15061 tgatacagac ccacatgtga gtcgtatttt gacttatctg ttagactatc caggatttgt
    15121 agttacagtt gattatggca attgggatca aattagtcac caacctgatg ctattgctaa
    15181 tttagctcgc ctttatacga gtctcgattt tgttgtttgt cacctctctt ttccacatgt
    15241 tgagcattca aatcgattgc gtgctgaact taatatgtgg aaaaacttca acaatatcta
    15301 tactgatatt tcagctattc aagatattga tgccccagat agttttcctt ttccaaaatc
    15361 agaacaaaat gtacgtattg ctaaagaagt tcttggggct aagcgtatca tttggggaac
    15421 tgattctcca tggtcagcga ccttcaatac ttacgaagaa ttagctactt ggctagagaa
    15481 tgttgatatt ttcagtcaag aggaactaga agatgttatg tacaataatg ctgagcgtgt
    15541 ttactttaaa gaagaacatg taaaggctaa cagagcaggt atagatgata caaccaaaga
    15601 gctcaatctt tattagaaga gttttcaagg tacaagagtt tcttgtacct cctttttaaa
    15661 tcaacaatga ggagacaaat tatgacttac taccttagca ttgactacgg cggtacaaat
    15721 accaaggcgc ttatttttga caaattagga caccaaatcg ctgtttcgag ttttgaaact
    15781 ttaaaaaatg aggctcaatc tggtcatcgt caagtaaacc ttgttaaaac gtggaatgct
    15841 ataacttctg ctattagaga ggttattcaa atctcaaaac tcagccctga gcagattagt
    15901 gcagtagcat gtattggaca tgggaaaggt ctttatctgc tagataataa gttggagcca
    15961 cttgaacaag gaattttgtc tacagataat cgtgccaaag atttggcgca atatttcgaa
    16021 tctaaacttg ataatatttg ggagttgact cggcaacata ttttcccttc acaaagtcca
    16081 gttattttac gttggcttaa agattatcag cccgaaacct ataaatcaat aggggcggtc
    16141 ctttctgcaa aggactttat tcggtataag cttacaggaa aagtacagca agaatatggt
    16201 gacgcttcag gtaatcattg gataaatttt caaacaggaa cttatgatcc agctatttta
    16261 gatttttttg gcattagaga gatagaaaac tcacttcctg aacttataga tagtgcagat
    16321 ttagttcctg ggggaattag ttctcaagca gcaaaagaga ctggtcttgt agaaggaacc
    16381 cccgttgttg gagggctctt tgacatcgat gcttgtgctc ttggatcagg tgttttagag
    16441 tcagatactt ttagtgttat ttcgggaact tggaatatta atacttatcc aagtttaaaa
    16501 ccagcaaagc aagatagtgg tcttatgact tcctattttc cagatcgtcg ttatctctta
    16561 gaggcaagta gccctacttc tgcagggaat cttgatttta tgttaaaaat gctcatgcat
    16621 caagaaattg ataacgctaa atctagtgga ggttctatct atgataattt agaagaattt
    16681 ctcactcata ctgatgccac acatcatgga cttattttct ttccgtttct ttacggtagt
    16741 aacacatcac aagatgctag cgcttgcttt tttgggctaa caactaaatc gacgaaatct
    16801 cagatgatac gtgcggtata tgaaggtatt gcgtttgcac ataagcagca tatcactgat
    16861 ttaataaaaa gtaggggcgg tgtgccaaaa ataattcgtt tctctggcgg agctaccaac
    16921 tcaccagcat ggatgcaaat gttttctgat atcttaaacc ttcctattaa aacagtagaa
    16981 ggcacagaat taggaggatt aggaggagct attttagcac gtcatgcttt agataagatt
    17041 tcgttaaagg aagcagtcca agatatggtt cgtgtaaaag ctatttataa acctcaatta
    17101 tccgaagtaa aggggtacga aaaaaaatat cacgcctacc aaaaattatt ggaaacactg
    17161 gatcctattt ggtcggaact cggtcatctg aataagtagg gatatcaaag gagttgttat
    17221 gaaacaagaa gatattatta tcaatagtat tgtattcaaa aaacagttag acaaaggttt
    17281 tacacaagtt cagctcatta agttagtaaa ggatttagga ttttcacgat ttgagatacg
    17341 tcaagaattg cttcaagatc cagatagaga gctacccgct ttaaaagcag aggctgattt
    17401 ttatgatatc aatctttact atagtgctaa tgaagacctt atcaaaggag ggaaagttaa
    17461 tccttatctt aataaaggcc ttaaagaagc atcacagtta ggagctcctt tcattaagct
    17521 aaatgttgga caaaccagaa acttaagtaa agaagagtta gaacctctta aagagatatt
    17581 gaaaagccaa actattggta taaaggtgga aaataatcaa gaccctaaag cagctacagt
    17641 ggagaattgc caatatttta tgactctagt gaaagaatta caaataccaa taagttttgt
    17701 ttttgatact gctaattggg cctttatcaa ccaagaccta taccaagcgg taaataactt
    17761 agcttgtgat accacctacc ttcactgtaa aaattttata caggtagctg gaaagcccca
    17821 cctctcgaag agcctcttcg aaggagagat taatctgact gatttgttaa agagtttctc
    17881 taattgtgag tatctagctc tagaataccc cacagagttg gagatattaa aaagggatgt
    17941 tcaacgttta atctctatca gtaatagtca aaacttttaa tgaaaaggct ttaaaaagat
    18001 gttataataa gtctgtatga attcgagtag aaaagaggta tattcttgat tattctcgat
    18061 aaaaaaagtt atgacctcct cttttaccta ttgaaattag aggaacctga aacagttatg
    18121 gcaattgcca acgcacttaa tcagtctaga cgtaaagtgt attatcactt agagaagata
    18181 aacgatgcac tgcctagcga tgtgcctcag attgttagtt atccacgtgt aggaatcttg
    18241 ctaacagaaa aacaaaaagc agcctgtcgt cttttattag atgaagtgac tgattatagt
    18301 tacgtcatga aaagtagtga gaggttgcag ctgtctttag tatctatcgt agtagctaag
    18361 gaccgcataa cgattgatag gttgatgcaa ctaaacgatg tttctcgaaa tactatctta
    18421 aacgatttaa acgaattaag aagtgagtta gcagagaaag aatataattt acagttacaa
    18481 tcaacaaaat gtcgtggtta ttttttagat ggtcatccat tgtccattat ccagtactta
    18541 tataagctct tagatgatat ctaccataat ggaagtagta gttttataga cctttttaat
    18601 cataaactgt ctcaagcttt tggtgccagc acttattttt ctaaagaggt tcttgattat
    18661 tttcatcatt atctcttcat ttctcaacga agtctaggta agaaaatcaa cagtcaagat
    18721 ggtcagttta tgattcagat tttgcctttt atactaatgg cttatcgtaa gatgcgatta
    18781 agtcctgaag tacagacctc tcttaatagt gattttagct tggtttggca acgtaaggaa
    18841 tatgagattg ctaaagagtt ggctgatgag ctggaagaaa attttcagtt atcactggat
    18901 gagattgaag tgggactagt agccatgctt atgcttagtt tccgcaagga ccgtgacaat
    18961 catttagaga gccaggatta tgatgatatg cgagctactc taaccagttt tttgaaagaa
    19021 ttggaagaac gatatcacct tcactttgtt cataaaaagg acttactaag acaacttctt
    19081 actcactgca aggcactctt atatcgtaaa cgttatggta ttttttctgt taatccttta
    19141 acagagcata ttaaagacaa atatgaagaa ctttttgcca taacctcgtc ttctgtaaag
    19201 cttttagaga aagcttggca aatcaaattg accgatgatg atgtagcata tctaacgatt
    19261 catttaggag gggaacttcg taatagtcaa caatctccta ataaacttaa gttagttatt
    19321 gtatctgatg aaggaatagc gattcagaaa cttcttttaa agcaatgtca acgctactta
    19381 acaaatagtg atatagaagc tgtttttaca accgaacagt atcaaagtgt gagtgatctt
    19441 atgcatgtag atatggttgt ctctactagt gacgctttag aatctcgttt tccgatgtta
    19501 gtagttcacc ctgttttgac agatgatgat attattcgct tgattcgctt ttctaaaaaa
    19561 ggtaactgtg caaatagtaa tcaatttacc aatgaacttg aaaaaacaat tgctcaatat
    19621 gtcaaggaag atagtgaacg ctacgtgctg aaatctaaga ttgagaaact tattcatcaa
    19681 gaattgctcc aagacgtcct tccccttcaa agtacagttt gttaaaatat aattttatgg
    19741 tagcctctca gcttttctga agacagaaaa agaagctggg gcttttttgg tgcttttaaa
    19801 caatgtgcta gatatttaat cgcaatgagt tcctctatga catgataatt aaagttgaga
    19861 aagctagtac ttttaatggg agtacttttt atttgtgctt attttaatta aaattatgag
    19921 tctaatttgg aacagacagc ccagttttga acacataaat gtgtgacact tggtcttttt
    19981 ttcaaaagta tttgaactta tactagtaga tgtaagaaac aattaatcag gaggaaataa
    20041 aatgcctaac gttaaagata ttactagaga atcatggatt ttatcaacat tcccagaatg
    20101 gggtacatgg ctaaacgaag aaattgaaga agaagttgtt gctgagggca actttgcaat
    20161 gtggtggtta ggaaactgtg gtgtttggat taaaacacca ggtggtgcta atgttgttat
    20221 ggacctatgg tcaaaccgcg gtaaatcaac aaaaaaagta aaagatatgg ttcgtggaca
    20281 ccaaatggct aatatggcag gtgttcgtaa actccaacca aacttacgcg cacaaccaat
    20341 ggttattgat ccatttgcta ttaatgaatt ggattactat cttgtttcac atttccacag
    20401 tgaccacatt gatatcaaca ctgcggcagc cattattaat aatcctaact tagaccatgt
    20461 taaatttgtt gggccatatg aatgtggtga aatttggaaa aaatggggag ttccagaaga
    20521 acgaatcatc gtgattaaac caggtgaatc atttgagttt aaagatatca aagttactgc
    20581 tgttgaatca tttgaccgta cttgcttggt gacacttcca gtagatggtg cagaagaaca
    20641 tgatggagaa cttgctggtt tagccgtgac tgatgaagaa atggctcgta aagcagttaa
    20701 ctatattttt gaaacaccag gtggaacaat ctatcatgga gctgactcac atttttcaaa
    20761 ttattttgct aaacatggta aagactacaa gattgacgta gctattaaca actatggtga
    20821 taacccagtt ggtattcagg ataaaatgac ttcaatcgat cttcttcgta tggcagaaaa
    20881 tctccgtgcc aaagtcatta ttcctgtaca ctacgatatc tggtctaact tcatggcttc
    20941 tacagacgaa atcttacaat tgtggaaaat gcgtaaagaa cgccttcaat atgacttcca
    21001 tccatttatt tgggaagttg gaggtaaata cacttatcca caagataaag atagaattga
    21061 ataccatcac ccacgtggtt ttgatgattg cttcgaacaa gaatcaaata ttcaatttaa
    21121 agcacttcta taatggctaa tgagagactg tatgtcgcat gatatgcagt ttttcaattt
    21181 taaaatatgt tactaaaatc acaattaaga ttaggagaaa taatgtcaga atttgataca
    21241 aaatcatatt tagaaaaact tgatgcatgg tggagagcag ctaattatat ttctgcagca
    21301 caaatgtatc taaaggataa ccctctcttg agacgagagc tggttgaaaa cgacttaaag
    21361 gttcatccaa ttggtcactg gggcactgta cctggacaaa attttatcta tgctcactta
    21421 aatcgcgcta ttaataaata cgatttagac atgttctata ttgaaggtcc aggtcatggt
    21481 ggtcaagtta tggtatctaa ttcatattta gatggttcat atactgaatt aaacccaaat
    21541 attgagcaaa cagaggatgg ctttaagcag ttatgtaaaa tcttctcttt cccaggtgga
    21601 attgcatccc atgctgcacc agaaacacca gggtcaattc atgaaggcgg tgaacttggt
    21661 tacgcacttt ctcatgcgac aggtgccatc ctagacaatc cagatgttat tgctgcgact
    21721 gttattggtg atggtgaagg tgaaacagga ccacttatgg ctggttggct atctaatacc
    21781 tttatcaatc cagtaaatga tggtgctgtt ctaccaatct tctatttaaa tggtggtaaa
    21841 atccataacc caactatctt tgaacgtaag acagatgaag aattatctca gttttttgaa
    21901 ggattaggtt ggaaacctat ttttgcagat gttgttgaac tctctgagga tcatgcggct
    21961 gctcatgctt tgtttgcaga aaaattagat caagctattc aagagattaa aaccattcaa
    22021 tcagaagcac gacaaaaacc agcagaagaa gctatccaag caaaattccc tgtcttagtt
    22081 gcacgtattc ctaagggttg gactggtcca aaagcttggg aaggaacacc aattgaaggt
    22141 ggcttccgcg ctcaccaagt accaattcca gtagatgccc accatatgga acatgttgat
    22201 tctcttttgt catggcttca atcataccgt ccagaagaat tatttgatga aaatggtaaa
    22261 atcgttgatg agattgctgc tatttcacca aaaggtgatc gtcgcatgtc catgaatcca
    22321 ataaccaatg ctggtattgt taaagcaatg gatacagcag attggaagaa atttgctctt
    22381 gatattaatg ttccaggtca aattatggca caagatatga ttgaatttgg aaaatatgca
    22441 gcagatttgg tagatgctaa tccagataat ttccgtattt ttggtccaga tgaaacgaaa
    22501 tcaaatcgtc ttcaagaagt gtttacacgt actagccgtc aatggcttgg tcgtcgtaaa
    22561 ccagattatg acgaagcttt aagtccagct ggacgtgtta ttgattccca attgtctgaa
    22621 caccaagcag aaggtttctt agaaggttat gttttaactg gtcgtcacgg cttctttgct
    22681 tcatacgaat cattccttcg cgttgtagat tcaatggtaa cacaacattt caaatggtta
    22741 cgtaaatcaa aaacacacac aacatggcgt aaaaattatc cagcgcttaa cctaattgca
    22801 gcttcaactg ttttccaaca agatcataat ggttatactc accaagatcc aggtatttta
    22861 actcatttag ctgaaaaaac acctgaatac attcgtgaat atttaccagc agatactaac
    22921 tcacttctag ctgttatgga taaagcgttt aaagctgaag ataaaattaa tttaattgtg
    22981 acatctaagc accctcgtcc acaattttac tctattgctg aagcagaaga gttagtggca
    23041 gaaggatata aggtgattga ctgggcttca aatgtgtcgc ttaatcaaga gccagatgtt
    23101 gtctttgctg cggcgggaac agaacctaac ttagaagctt tggcagctat tagcattctt
    23161 cataaggctt tcccagaact taagattaga tttgttaatg tattagacat cctaaaactt
    23221 cgtcatcctt cacaagatgc tcgcggttta tcagatgaag agtttaacaa agtctttacc
    23281 acagataaac ctgttatttt tgctttccat ggttatgaag atatgattcg agatattttc
    23341 tttagtcgtc acaatcataa tttgcatacg catggttacc gtgaaaatgg tgacatcaca
    23401 acaccatttg atatgcgcgt gatgtcagaa ttggaccgat tccatctggc acaggatgca
    23461 gctcttgctt ctttaggaaa taaagcacaa gccttcagtg atgaaatgaa tcaaatggtt
    23521 gcttatcaca aagactatat tcgtgaacac ggagacgata ttccagaagt acaaaattgg
    23581 aaatgggaaa atatcaagta gtcacttttt tcaggcctat acttacaaac aaaaacactt
    23641 ttagattttt tctaagggtg ttttgttatt ttatagaaga atgaccgctt tttcaaaagt
    23701 attaagtggt cacttttaaa gttgtaatat taatactaat aagtctttaa ataattcaat
    23761 aatgccataa caataatggt gtgggctaat taaaatatct ctaagcaaag gtgttgacat
    23821 aaagagacca tacatgatat tatagttatg ttctgtttcg gcagtttatt tttttgagta
    23881 aaaaagtgac taccctttta cgataactta acgtcacttt atagataaga cacataacct
    23941 taaaaataag atacgaaatt tttagtagga gataagaatg acaaatattt tagaagtgaa
    24001 gaatttaacc aaaatttttg ggaaaaagca aaaagctgct cttgaaatgg ttaagcaagg
    24061 aaaaagtaag acagaaattc ttgaaaaaac aggagcgact gtaggtgtct atgatgcgag
    24121 ttttgaaata aaagaaggcg aaattttcgt tatcatggga ctttctggtt caggaaaatc
    24181 aactttagtt cggatgttaa accgtttgat tgacccatca tcaggaaata tctatttaga
    24241 tggtaaagat attgcaaaga tgaatgtaga agatttacgt aacattcgtc gtcatgatat
    24301 caacatggtt ttccaaaatt ttggtctttt cccacatcgt acaattttag aaaatacaga
    24361 gtttgggtta gagatgcgtg gtgtttctaa ggaagaacgt acaacgttag ctgaaaaagc
    24421 tttggacaat gcaggtctat taccgtttaa agatcagtac cccagtcaat tatcaggagg
    24481 aatgcaacag cgtgttggtt tagcacgtgc ccttgctaac agccctaaaa tccttctgat
    24541 ggatgaggcc ttctcagctt tagacccatt aattcgtcgt gaaatgcaag atgagcttct
    24601 tgatcttcaa gacacgaata aacaaactat tatttttatc agccatgatt tgaatgaagc
    24661 gttacgtatt ggtgatcgta ttgctctgat gaaagatgga gaaatcatgc agattggtac
    24721 aggagaagag attttgacta atccggctaa tgattttgtt cgtgagtttg ttgaagacgt
    24781 tgatcgctca aaagtgttaa ctgctcaaaa tattatgata aaacctttaa caactgtttt
    24841 agaaattgat ggcccacaag ttgctttaac aagaatgcat cgtgaggaag tctcaatgtt
    24901 aatggcaacg aatcgtcgtc gccaacttct gggatcccta actgcagatg ctgcaattga
    24961 agctcgtaag aaaggtttgc cgctttctga agtgattgat aaagatgttg taacggtctc
    25021 aaaagataca gttattacag atatcatgcc tttaatttat gattcatctg ctccgatagc
    25081 cgttactgat gataatgata gactcctagg ggttatcatt agaggacgtg tgatcgaagc
    25141 acttgctaac gttcaagatg aaacagtagt agaatcgcca aaagaaacgg tagaagctta
    25201 atagagagga caattcattt tggaaaattt attacaacat aagttaccgg tcgcaccatt
    25261 tgtagagtca acgaccaatt ggattacgaa aactttttct ggtttatttg actttatcca
    25321 gacaatcggt aatgcattga tggattggat gacaaaaaca ttactattca ttaatccttt
    25381 actttttatt gtactaatta caattgctgt atttttctta gctaagaaaa aatggcaatt
    25441 accgacattt actttcattg gtttgctatt tatctataac caagggctgt gggaacagtt
    25501 gattaatact tttaacttgg ttttagtagc aagtttaatt tctattatta ttggtgtacc
    25561 actaggtatt tggatggcaa aaagtgataa agttaaacaa gttgttaatc caattcttga
    25621 ctttatgcaa actatgccgg cattcgttta ccttattcct gcagtagcct tctttggtat
    25681 cggtatggta ccaggtgttt ttgcctctgt tgtatttgct ctgcctccaa cagtgcgctt
    25741 tactaacttg gctattcgtg aaatcccact tgaattaata gaagcatctg attcatttgg
    25801 ttcaacagct aagcaaaaat tattcaaagt tgaactacca cttgctaaga acactattat
    25861 ggcaggaatc aatcaaacta tgatgttggc tctttctatg gtggttacag gatctatgat
    25921 cggtgcacct ggacttggtc gtgaggtgtt atcggcgctt caacatgctg atattggtac
    25981 aggatttgtt agtggtctat cgctagttat tttagcaatc gtgttagacc gtgttagtca
    26041 attctttaat tccaaaccgg gtgagaagca agctaaaaca tcaaaagtta aaaaatgggt
    26101 aggtttaggg gccctggctc tcttcattct agcagctctt ggtcgcattg ttgtcaatat
    26161 gacttcagga aatgaagcca aaggtcaaaa agtgaagata gcttatgtcc aatgggattc
    26221 agaagttgct tcaaccaacg ttattgctga agttttaaaa tctaaaggat atgatgttga
    26281 attgacacca cttgacaatg cggtcatgtg gcaaacagtc gccaatggga atgctgattt
    26341 tacaacaagt gcttggttac caaagaccca cgggcaatat tttaataaat acaaaaatag
    26401 tttagatgac ttaggtcctc acgttgagaa cgtcaaaata ggtttagttg ttcctaaata
    26461 catgaacgta aatagtattg aagaattatc aaatcaagca gataaacaaa ttacaggtat
    26521 cgaaccaggt gcaggtatta tgaagtctgc taaacaatcc cttaaagatt atccaaacct
    26581 ttcaagttgg aagttgttat cagcatcaac aggagctatg acaacaacct tagggaaggc
    26641 tatcaaaaac aaagatcaag tagtcattac aggttggtca cctcactgga tgtttgctaa
    26701 gtatgattta aaatacctta aagatcctaa gaaatcattt ggtggtgaag aacatatcaa
    26761 tacaatagca cgcaagaatt tgaaaaaaga tatgccaaaa gtatataaaa ttatcgataa
    26821 gttcaagtgg actaaagaag atatggaatc tatcatgtta gatatggata aaggtatgga
    26881 gcctgctaaa gcagctcaaa aatggattaa aaaccacaaa aaagaagttt cagagtggac
    26941 taaatagtat agaaaaaaac acgtctctct tagagcgtgt tttttaatgt gacttaagtc
    27001 gttttagcta tttaatataa gttttatatt attttaatac atcaaaagtg agagaatgaa
    27061 agaaatggca ggttataaag tatta
//
LOCUS       DATRME010000019        26790 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRME010000019 DATRME010000000
VERSION     DATRME010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26790
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            114..1476
                     /locus_tag="VBC23_000831"
                     /pseudo
     CDS             114..1476
                     /locus_tag="VBC23_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1648..2262)
                     /locus_tag="VBC23_000832"
     CDS             complement(1648..2262)
                     /locus_tag="VBC23_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6842797.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(2395..2820)
                     /locus_tag="VBC23_000833"
     CDS             complement(2395..2820)
                     /locus_tag="VBC23_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6842798.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(2947..7353)
                     /locus_tag="VBC23_000834"
     CDS             complement(2947..7353)
                     /locus_tag="VBC23_000834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6842799.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(7476..8060)
                     /locus_tag="VBC23_000835"
     CDS             complement(7476..8060)
                     /locus_tag="VBC23_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6842800.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(8185..10038)
                     /locus_tag="VBC23_000836"
     CDS             complement(8185..10038)
                     /locus_tag="VBC23_000836"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6842801.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(10130..11389)
                     /gene="rseP"
                     /locus_tag="VBC23_000837"
     CDS             complement(10130..11389)
                     /gene="rseP"
                     /locus_tag="VBC23_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6842802.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(11420..12214)
                     /locus_tag="VBC23_000838"
     CDS             complement(11420..12214)
                     /locus_tag="VBC23_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6842803.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(12229..12981)
                     /locus_tag="VBC23_000839"
     CDS             complement(12229..12981)
                     /locus_tag="VBC23_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6842804.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(13148..13489)
                     /gene="yajC"
                     /locus_tag="VBC23_000840"
     CDS             complement(13148..13489)
                     /gene="yajC"
                     /locus_tag="VBC23_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6842805.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(13566..13910)
                     /locus_tag="VBC23_000841"
     CDS             complement(13566..13910)
                     /locus_tag="VBC23_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6842806.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(14101..15264)
                     /locus_tag="VBC23_000842"
     CDS             complement(14101..15264)
                     /locus_tag="VBC23_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6842807.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDICPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(15289..16626)
                     /locus_tag="VBC23_000843"
     CDS             complement(15289..16626)
                     /locus_tag="VBC23_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6842808.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            16780..18306
                     /locus_tag="VBC23_000844"
     CDS             16780..18306
                     /locus_tag="VBC23_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6842809.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGS
                     "
     gene            18308..18988
                     /locus_tag="VBC23_000845"
     CDS             18308..18988
                     /locus_tag="VBC23_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6842810.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(19009..20004)
                     /gene="galE"
                     /locus_tag="VBC23_000846"
     CDS             complement(19009..20004)
                     /gene="galE"
                     /locus_tag="VBC23_000846"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6842811.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(20092..21699)
                     /locus_tag="VBC23_000847"
     CDS             complement(20092..21699)
                     /locus_tag="VBC23_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6842812.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(21828..22961)
                     /gene="ugpC"
                     /locus_tag="VBC23_000848"
     CDS             complement(21828..22961)
                     /gene="ugpC"
                     /locus_tag="VBC23_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6842813.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(23062..23913)
                     /locus_tag="VBC23_000849"
     CDS             complement(23062..23913)
                     /locus_tag="VBC23_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6842814.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSYSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(24008..24904)
                     /locus_tag="VBC23_000850"
     CDS             complement(24008..24904)
                     /locus_tag="VBC23_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6842815.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(24958..25935)
                     /gene="lacD"
                     /locus_tag="VBC23_000851"
     CDS             complement(24958..25935)
                     /gene="lacD"
                     /locus_tag="VBC23_000851"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6842816.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(25937..>26790)
                     /locus_tag="VBC23_000852"
     CDS             complement(25937..>26790)
                     /locus_tag="VBC23_000852"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6842817.1"
                     /translation="VTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRSGL
                     DALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDAAT
                     IITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPNLE
                     ELTQLIGRPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPKIE
                     VINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQELIS
                     QIQVKEV"
BASE COUNT         8723 a         5366 c         4259 g         8442 t
ORIGIN      
        1 ttaaaaatat agaggaattt aagacgataa cccaaaaaag gctaactgag agaggaaagt
       61 tcccatatga taccgttcat tctacgtttg aaattaagga tgaaaacttt atcatggaaa
      121 gattgaaatc atcagggtta tctatgggta aaccagttga ttatatgggc gtaaatggta
      181 tccctattta cactaaaaca ttatctatag ataataagtt tgcctttgaa aataattcaa
      241 aagattcttc atattcttca aacattaata ttagtgaaga taaaataaaa gaaaatgacc
      301 aaaaaattct agatttaata gttaaatctg gagctaataa tcaaaactta acagatgaag
      361 aaaaagtaat tgctttcact aaatatatag gtgaaataac taattatgac aatgaagctt
      421 acagagctag aaatgtcgac acagaatatt acagagcttc agatttattt tctgtaacag
      481 aaagaaaact tgctatgtgc gttggctata gtgtaacagc cgctagagca tttaatatta
      541 tggggatacc tagttatgtc gtttctggta aaagtcctca aggaatatct catgcagctg
      601 tgagagcgta ttataataga agttggcata tcatagatat cacagccagt acatattgga
      661 aaaatggtaa ttacaaaaca acgtattccg attttattaa agaatactgt atagatggtt
      721 atgacgttta tgatcccgca aaaacaaata atagatttaa agttaagtat atggaatcaa
      781 atgaagcgtt tgaaaattgg atacataata atggaagcaa atctatgtta tttattaatg
      841 aatctgcagc tctaaaagat aaaaaaccaa aagatgattt tgtaccagta acagaaaaag
      901 aaaaaaacga gctgatagat aagtataaaa aattattgtc acagattcct gaaaacacac
      961 aaaatccagg agaaaaaaac ataagagatt atttgaaaaa tgaatatgaa gaaatattaa
     1021 agaaagataa tttatttgag catgaacatg cagaatttaa agaaagtcta aatttaaatg
     1081 aatcattcta cctacaatta aagaaagaag aaaagaaacc gagtgataat ctcaaaaaag
     1141 aagaaaaacc aagagaaaat tcagtaaaag agagagagac tccagctgaa aacaatgatt
     1201 ttgtttcagt aacagaaaaa aacaacttga ttgataaata taaagaatta ttatcaaaaa
     1261 tcccagagaa tacacaaaat cctgggggaa aagaatatac gaaattatct agagaaagaa
     1321 tatgaagaac tactacaaaa agatagatta ttcaagcatg agtacacaga gtttacaaaa
     1381 agtctaaatt tgaatgaaac attctattca caattaaaag aaggagaaat gaaactaagt
     1441 gaaaatcccg aaaaaggaga aacgaacacg aattaacctt tcaaatatct acatcaaata
     1501 acagaagtct ccgtatcaag cgaattgtag cttttttgaa tatactaaaa acactcctac
     1561 ctctatttta ctagaagtag gagtgttttt ttcattttag ttcatagatg cagtgccatt
     1621 gctatccttg cctttttttg tacagtatta tccgatgact ttaaagcggt catcatcatt
     1681 catgaattct ttttctcctg tttcagcttc gattgaacca aaatttagtt gtccacgaag
     1741 tttccaacta gctggaacac cataaacagc ttgtactgat gcatcaataa ctggattgta
     1801 atgttgtaaa ttaccaccaa gtccaagttc tgcagaaagg gctgtccaag tatttactga
     1861 ggcaatacct gttgattgct cagaccaaac tggaaagtta tccgcataaa gaacgaattg
     1921 ttcttgaaga gatttgacaa catcttgatc ttcaaagaag agtactgttc cctttgaagc
     1981 tccaaaacta gctaattttt ccttagttcc agcaatagct gtctctggag caccttgtac
     2041 tttcattgtt tctaccaaat cattagcaac caattcatcc cagaacttag taacttcatc
     2101 attcaataaa atgacaacac gggaagtttg actattaaaa gcagatgggg attggcgaac
     2161 tgcctcttta ataatctcta ctattttctc atcagaaact tctgtattgc gaccaagagc
     2221 gtaaatagag cgacggtttt ttaattcttc caaaaatgac atattttttc tccttgttct
     2281 tcaatctcag atatcttact acatttgttc tagtgacata tgaattagat taaagttatt
     2341 tattactatg tagtaattat attaccacta tatcgtaaag tcaaagaatg tgactcacaa
     2401 ggttttaaat ttttttaaaa ggttaatcaa gtcttcttgc tctgtttctg ttaagactga
     2461 aaaagcatct tctacacgtt taatatgctc tggtagagct ttttttatca cttcttctcc
     2521 ttcagttgtt aaactcacca aaaaggctct cttatcattc ggacaagaat ggcgtaatac
     2581 ccatcccttc ttttccatat ttttaataac aacagtcata ttacctgatg tagcaagcat
     2641 attttcaatc aattctccaa ttttcattgt gcctttagca tagagaacat ccaaaactgc
     2701 aaactgagtt ggtgttaatt caaattcttt aaatgattct gagacttttc cgtctattgt
     2761 tctaaatgct tttcgcatga caaccataga tttaaccgct gaatttttaa agtttcccat
     2821 ttcatctccc aaacgtattt tttcttattt tatcactaat tagttatttt aagcaagaat
     2881 atcatctttg ccctatcaaa agcagctggc aaataataag gttgagtaac tactcaacct
     2941 tattgcttag aagaaatcat caaaaagact caactgattg tcttctggta aatttcccaa
     3001 aatacccatc tcatcaaatt tttcaactaa ggtacttgag actcctcctc gtttacgaag
     3061 ctctgttttt gataaaaatt caccatcttc acgtgctcga actatctgtt ttgcaacgtt
     3121 ttctcccaag ccttccatag caacaaatgg agggattagc gtatcctctt caataataaa
     3181 gtcagtcgca tggctacgat aaagatctaa tttcccaaat ttaaagccac gctcaagcat
     3241 ttcattaacc aactctaatg tcgtaaaaag atcattttcg acattcgtcg cctcattacg
     3301 ctgacgcttc tcagtgatat ccttcatgcg cgccttaacg gcatcaagtc cagcactcat
     3361 tgtgcgtaat tcaaaagctt tggcgcgaat agaaaaataa gcacaataat aaaatattgg
     3421 ataatgtact ttaaagtacg ccacacgtaa ggccattaaa acataggctg ctgcatgggc
     3481 tttagggaac atatacttga tttttccaca cgattcaata taccaatccg ggacattatt
     3541 atcacgcatt gcttggatat aaccgttacg ctcatcttct gaaattttca accataaccc
     3601 cttacgcacg cgctccatga ttgtaaaggc catctttggt tgtagaccag cgtgcattaa
     3661 ataaaccatg atgtcatcac gacaaccaat aacggttgat aaggtagcaa taccttcttt
     3721 gattaagtct tgagcattac ctaaccaaac atccgtacca tgtgatagtc ctgacagttg
     3781 caacaattct gcaaatgtgg ttggatgggt ttcgttcacc ataccacgaa caaagttcgt
     3841 accaaactca ggaataccaa gcatccctgt aggagttcca atttgctctt ctgtcactcc
     3901 taaaacttcc gtaccagaaa ataacttcat aacgtctgga tcatctggaa ggatatttga
     3961 tggatcaatt cctgataaat cctgaagttt acgaatcata gtaggatcat catgtcctag
     4021 aatatctaac ttcaagacat tctcatcaat atcatggaaa ttgaagtgag ttgtctgcca
     4081 agcagctgtc atatcatctg ctggatattg aaccggagta aaatcataaa catccatata
     4141 gttaggaata acaacgatac caccggggtg ttgacctgta gaacgtttga ctcccgcagc
     4201 accagtcgct aaccgttcga cctctgcatc gttataaaat ttattgtaat ctcgttcgta
     4261 acctttgaca aaaccaaagg cagttttttc agctacggta cctaccgttc ctgcacgaaa
     4321 agcatattct tctccaaaga tatcacgaac atctaagtga gcagaaggtt gatcatctcc
     4381 agagaaattt agatcaatat caggaacctt atctccatca aatcctaaga aagtttcaaa
     4441 cggaatatcc tgtccatctt tcttgtacaa agtaccacat ttaggacaat ttttatttgg
     4501 taaatcgtaa cctgaaccac aagaaccatc tgtgataaat tcagaatgct gacaatttgg
     4561 gcaaacataa tgaggtggca ttggattaac ttcagtaatt cctatcatgg tcgctacaaa
     4621 actcgaccca acagacccac gtgatcctac tagataacca cgttcattag aacgttgcac
     4681 caacatttgc gaagcaagat agataacggc aaatccatta ccaagtatag atgctaactc
     4741 tttttcgata cgtaaatcaa taatatcagg aagaggatta ccatatattt cgaatgcttt
     4801 agcataagtt aattcagcaa ctcgttcttc agcacgatca acaaacgggg tatagagatc
     4861 cccttttaca acttcaacgt cttcaaatcg atctgcaaaa gtattcgtat tggttacaac
     4921 gatctcatag gctaaatctt taccaagaaa ggcaaattca tctaacatct catttgttgt
     4981 acggaaatgt gcttttggca aaggcgctgg ttgagcatcc tccccacgac cgatagtcct
     5041 attaatcatg gcaccttgtc caagactacg aacaataatc tcacggtaaa tctcatcttc
     5101 aggctcaata taatgaacat ttcccgtagc taaaacaggc ttatctaaac gccgaccgac
     5161 ctctattagg tctctaataa tttgttgaat ccccgcctca tctttaatta aatcacgaac
     5221 caccaagggg cgataaatag caggtggcat gacttcaata aaatcataat atttagctag
     5281 ggtcactgct gcatcaattc catttgataa caaggcatca aaaacttctc catcagaaca
     5341 ggcagtacct agcaagagac cttctctgtg agcatctaaa actgatctag gaattcttgc
     5401 caccccttca aaatatttaa catttgaaag gctaactaac ttaaatatat ttttaagtcc
     5461 aacctgattt tggacataaa tggttgcatg ctttatgcgt gcctttttgt aagagtcttc
     5521 agctaccaat ttagtattga gctccatcaa attagtcaca tcacgatttt cccgcgcctc
     5581 ttttaagaaa ataaagagta agcgacccgt tgcttcagca tcgtagttgg ccatatgatg
     5641 atgttccaaa gcaacttgga aacgtttggt tagaggcccc aaaccatgac gcttatattc
     5701 aggataaaga tttcttgcaa attctaaggt atcaatgacc ggctgtgtga tcaaaggcag
     5761 attatgacgt tcataattcg cattcataaa accaacgtca aaggttgcat tatgcgcaac
     5821 taaaaccgta ccttgacaaa aattctgaaa ttcctgaagg acttgtaaaa taggttttga
     5881 acctctgaca tgattatccg taatacctgt caactcagtt gtaaagtcag aaagaggatg
     5941 gcctgggtca ataaactcat caaattgctc aataatatta cccttgaaca tttttgatgc
     6001 tgcaatctga attaggtcat tattagctgc agaaagacct gtagtttcca catcaaatac
     6061 aacataagtt gcttcgtgca aattcatatc aacttcgttg taggaaatag gcactttatc
     6121 ttcaacaata tttgcctcta atccaaaaat tgcttttata cctgcttttt ttgctctgtg
     6181 gtagccgtgc ggaaaacttt gaacattggc atggtcagta attgcaatag ctgggtgtcc
     6241 ccacttagct gcagtatcaa tgaggctctc gacagttggt aaagcatcca tcgttgacat
     6301 attggtatga gcatgaaatt ccacacgttt ttgatctgct ggcattaagt ctttacgttc
     6361 atgatgcaca atctctttga tatcttgaac attcattgtc aaagatttag taaagttatt
     6421 gttctcaata ttccctctga cccgcaacca cgaaccttta gaaatcatat cgtacttctt
     6481 caattcttca tcatctttag cccacttttg catagcaaaa cttgaggtat aatcagtcat
     6541 cttaaaatta atgatatgac gtccagtgcg ggttgttttt cgttcaacac taaaaaccat
     6601 tccttcaaaa acgatacgat tttcttctgt ggtcacctca atcatgggtg taatctctgc
     6661 tttttcaaag ccagcttgac gttgtttgat acgctctttg aaatcataat tttgagttgg
     6721 tgtcacttct tctgatggag gcgctgaatc ttctagtgat ttttgtgctt ccaaggcttg
     6781 catagcctct tggttagctt tttcaagcaa ttgctcgcgg ttagtttcaa atgatgattt
     6841 taagttttgc gtcatttgtt catcagaaac catatcaata gcaagtttac caaatccaaa
     6901 caatgaaaat tgttgttcca agcgcggcaa atgattctga cgaaaatgat tattattgac
     6961 aaattgaggt gctgaaataa tcatctgact gccattgtaa tgaactttta attgtgaaaa
     7021 agaactctta aaacttgcac tattacacaa gggttcacaa aaagcttgtt gataataatc
     7081 ctgtagcaga tcatcagaaa aatcaatagt ttctgctctt atatcaaaag tagcctttat
     7141 atctgctttt tcaaaactat tcactaaacg agtttgtaat tcacaataca cctcaattgg
     7201 caataattct ggaaatgaaa aatgaaattc ccataagcgt gatagagaat gaacctttac
     7261 ctcgataata tcagcagacg aaaaaacact tgaattctta atttcaagcg gcatttcaat
     7321 ctgatccata agtttcttga acaattctga catagttttc tcctgaatat cttacttcca
     7381 ttttatcaca tatctgtaaa tatttcgaga taacaaaagg aggctgttaa aaaaggcatt
     7441 tattagcctt cttaacaaca ctctcttaac tgagactatt tatcataagc atctagactg
     7501 taggtttcaa taatttggtt taatttacta gcataagcag tatctgtcgc ataaagacct
     7561 gttagagctg cagtagcatc cttataagaa gaagtatttg atttccaaac agatgcatac
     7621 ttttgactag atactaactc agcataatca tatagtgaag cagaataatt tggataggcg
     7681 cgaaaaggag cttggatttg agtcatattg cctttcccat catcttctaa agtaggcatt
     7741 tggacagatt tacctttata ttctcctttg atgccaaaga ggttataatt aggagcctta
     7801 gacaaatctg attgtccact ggatgattcc aaaatagctt gtgctaataa gactgacgca
     7861 tacaaatcat aggtcttaga aatttcaata gctgttggag aaatcgtttg aataaaagtc
     7921 gtcgtttgat ccgatgcact agtgactgtc ggttcgacat tatcattttt ataattgtaa
     7981 aaccaaaccc cacccaaacc aaaaactaat agtgttgttg ttaacctcaa taccaattta
     8041 tctttttttc gacttttcat gatggctcct ttctaaaata tcatttcatt ataacaaaag
     8101 gcagttgctt tttaccaata agaaccatac taaatttcac tccatataaa aaagttagca
     8161 catcagacta acttttttat cggcttattt ttttgttaga atctctaaag tttcaattaa
     8221 gttatcagcg tgaacctcaa tcgtatcacc acttgcttta attttcactt caacgatacc
     8281 ttcagaagct ttcttcccaa cagttactcg aattggaagt ccaattaagt cactgtctga
     8341 gaatttagaa ccaacacgtt cattacgatc gtctgtcagg acttcataac ctttaagcat
     8401 caaatcagct tcaattttct cagtaagatc ttgtgactct tgatctttga cattgacagt
     8461 aatcaaatgt acatcaaatg gggctaattc ttctgggaaa ttaatccccc aagcaaagcg
     8521 ataagctcct tttggtgtct tattcacaaa aagtcgggca tgttgttcaa tgacagctga
     8581 gagaatacga ctaacaccaa taccataaca ccccataaca ataggatttg agcggccatt
     8641 ttcatcaagg atgtttgctc ccatactatc tgaataacgg gtacctagtt taaagatatg
     8701 tccaatttca ataccacgag caaatttcag agtaccttta ccgtctggag aaatctcccc
     8761 ttcttttact tcacgaatat caacgtattc agctgtgaaa tcacgttctg gattaactcc
     8821 agtaaaatga taaccatctt ggttagcacc agacacagca ttagcaaggt cttgaacttt
     8881 acgatcagca ataattttaa cagactcagg taggttaact ggcccaagag aaccaaaacc
     8941 tgcaccaaaa atctcttttg cttgctcttc acttgcaggc tctaagaaat cagccgctaa
     9001 ataatttttt aacttcacat cattgacttg atcgttacca actagtaaag caaccacggg
     9061 ctgttcatca gcaataaaca atagtgtctt gatagtttga ttttcatcaa ttgataaaaa
     9121 tccagctacc tcatcaattg atttgcagtt aggagtttct acttttgtta cttcttcaaa
     9181 ggtagcagcc ttagtagatg gtttgtactc attcgttgcc atctcaaggt tagcagcata
     9241 gcttgattca gtggagtatg caatagtgtc ctcaccagaa actagccaag ccgagagttc
     9301 tgccttaatt tcttctaaaa catcctctgg aatatcatca atagaaggaa tagttttatc
     9361 aaggactaac caacgattta aatctgtacg attgggagtt actgccataa attcttggct
     9421 atctttacct cccatagcac ctccgtcacc tataatgcct ttaaagtcaa gtccagcacg
     9481 tgtgaagatg gcttcatatg ctttacggta atcttcgtaa gtgacatcaa ggtcttcata
     9541 atctttgtgg aagctgtatc catctttcat gataaattca cgtgttcgaa gaagtccatt
     9601 acgaggacgc ttctcatcac gatatttaga ctgaatttga taaaggttta gtggtaattg
     9661 cttataagat tttactgcat cacgaacaag agttgtgaaa gtctcttcat gagttggtcc
     9721 taagataaaa tccgattgat cacgattctt taatttataa aggtcttcac catatgtttc
     9781 gtagcgccca gattcacgcc ataaatctgc tgtaagaaga gctggagcta acatttcaac
     9841 tgcaccaatc ttctcaaatt cttgacgcat aattgtttta aatttttcaa tagtacggtt
     9901 agctagaggc aaataagcat agattccagc cgaaacttgg cggacatatc ctgcacgaac
     9961 cataagagca tggctgataa cctgagcatc acttggcatt tcgcgaagtg ttggaataag
    10021 cattttactt tgtttcatta caataagata cgactaatca aagttgattc tttttcaatt
    10081 gagaattgca attgaaaaat tcgtatttcc tttcacgttt tattatcttt tagaagaaag
    10141 ctcgcatgat atcgttccag gttacagcaa tcattaatac taacataact gctaccccag
    10201 cgagcgtaat ataagtttct gtttcttgtt taagtggttt gcgacgaatg gcttctaaaa
    10261 tattcatcac tatcttaccc ccgtcaaggg ctggtatagg gatgagattc ataataccta
    10321 agttgataga gattaatccc attaagttga gaactgtgac gaaaccattt ttagcagctt
    10381 ggctactagc ctgatagaga gctactggac ctcccaattt attaatacta aaatgagcaa
    10441 taagtttctt taattcattt aaaattctaa agaaactttc ccatgctagt ttaaatccac
    10501 ctaaaagctt gtctttaaag cttgtcttta gagctggcat aatgccgatg atgtaggatt
    10561 tatcaacttt ctgtggctta acgttgatcg ttttcaccac ctctttgctc ttaattttaa
    10621 gggcaagttt ttggtttttt tctaaatgac tagttgactt ttctactgct gctgtcaact
    10681 gttcccagtt agaaactttg tgcgacccta tttgtaaaat acggtcatta ttttttaaac
    10741 ctgcactagc agcaggacca ttttctgata cacggacctg attggttgat aaatcttgaa
    10801 cacctccttg aataaaagca agtgcaataa agactacaag acctaaaata aaattattca
    10861 tcggacctgc aaaattggta attaagcgcc cccatacaga agcattctga tactgcacat
    10921 ctaagggagc aatacgaatt tcagtaccat cctcctcaat gatagtagca tcgtgatcaa
    10981 cactataggt ttttgtctca cttaagacca atcctgtgat agttagttta tcttctaaat
    11041 cataagctgt tacattgatt ggtaaagaag tattgtctaa ttgttttcct gataaattaa
    11101 tacgagttac aatgccttct ttatttaaag taaggctagc cggcgtccct gtcttaattt
    11161 cagtcttatc atcaccccaa ccagccatac ggacataacc acctagcggt aatatacgaa
    11221 tagtataggt tgttccttct ttatcaatgt gagaaaagat ttttggaccc ataccaatag
    11281 caaattcacg aacaagaatt cctgatttct ttgcaaaata gaaatgccca aattcgtgaa
    11341 caactacaat gacaccaaaa ataataataa aggttaaaat tcctagcatt caattcctct
    11401 ctaaatacaa tatattattt tagaataagc caaagaagtg catgattggg aaaacaaaaa
    11461 tcatactatc aaagcgatcc aaaatccccc catgacccgg aatcaattta cccgaatctt
    11521 taacaccaaa atgacgttta atactacttt caactaaatc tccaaattga ccaaaaatgc
    11581 taaagatagc cactaaaact aacatgacta ggaagctatg aggcgcatag acggtcttat
    11641 caaaaagcat aaaaaagaaa gctaccacaa tggctgaagc aattcctccc agactccctt
    11701 caattgtttt atttggagac actgaaggta gtagtttgcg ttgtccaaat tgacgaccaa
    11761 tcatataagc accgatatca gtcgcccaga caataaataa agctaacaac accttatcta
    11821 ttccagccat acgagcactg actagatttt gaaagccaat acccacataa aaactagacg
    11881 caattggaaa ggcagcatct tcaaaactgt aagaattact gttcaaaaca gtacctgcca
    11941 aaatcataaa aataacaata ccgtatgctg ataaactagc gtcaactggt aaaaaggaca
    12001 aataactatc taaaggaact gttaaaacaa aggctcctat catagctaat gcaccttcaa
    12061 aggaaaatat ttctaaacga cgcatacgaa gtaattcaga aacaccaatc atagctaaaa
    12121 gtccaactaa aaattggaaa ggcaaaccac ccattactaa aaaagggata aatatggcaa
    12181 gcgcgacagc tccccaaata acacgttctt tcattttctc tcttctttct atacactacc
    12241 aaatcgacga tggcgttgat tataatccac aatggcctta tgtagttcgt cttttttaaa
    12301 atctggccat aaaacgggag taaagtaaaa ttcactataa gctgattgcc aaggtaaaaa
    12361 gttactcaag cgtaactctc cactagtgcg gataatcaaa tcagggtccc tataaagata
    12421 gggtaagcta tttgtcatta aatgatttgc tactaaatcc tctgtaatat cgtcaggatt
    12481 caacttagct tccaaaacat cctgagcaat ttccttaatg gcattagtga tttctgaacg
    12541 tccgccataa tttagcgcaa aattcaatac gagccccgaa ttatgttttg tcctcaaaca
    12601 agcgcgttgc attgcatcgt atgttgcctt aggtaattta tgagtatctc cgatgacctg
    12661 tacacgtaca ttatttttat ctaactctgg tacatattta tcaaaaaatt caaccggaag
    12721 gttcataata aacttaactt catcatctgg acgtgaccaa ttttctgtag aaaaggcata
    12781 gacagttagt actttaacac ctaattctga agctgctatt gtaacttctt gaagagcatc
    12841 catccccgcc ttatgtccca taacgcgtgg ttttaaacgt ttttttgccc aacgtccgtt
    12901 accatccatg ataataccaa tatgtgctgg tattttctcc aaaggcattt ctacttgttt
    12961 ttttctttta aatataaaca taatgttcct taatttagca atcgtttttc ttatcagccc
    13021 ttttaaaaga gctggaaata tcccatacta taacatttta ccacatttta acttattttt
    13081 gagtattaaa atgccatttg tgacagcaaa ctaaaaagac cagaacaatc atctggtcct
    13141 acatatctta ttcagtttcg atggctgttt ctttattttc gtcttcattg acttcaataa
    13201 tcttaacatc ttcaactggt tctgttggtg ttgctgcctt agagacaaca gatttgatag
    13261 ctgccaactc aaacgtcaag tagacaccat caacatctaa aaccattcgt tgtgcttcag
    13321 tattaacttc atcaactact ccaaaaagac caccaattgt gacaatttca tcaccttttt
    13381 gaacagcatt caattgtttt tgacgttctt gtgcttgttt cttttgctga cgttgcatga
    13441 agaacatcat accaaccata acaacaagca taataatagg taagctcatt tgattctcct
    13501 ttccgtgttc tacgtaatca ttactatagc agatttatgc tatatctgca aggagatact
    13561 gtctcttagc caataaattc acggatttct tcttttgtca tttttgaatc gcacttcacc
    13621 ttgatagttc cgtttttaac aaccatcaaa cctggcactg taggaatgtc atatttattt
    13681 cgaaaatctt tatttgcagt ccaatgatca aaatcttcac tatctaaatg gaataattca
    13741 aattcatctt catcagctac ttctacaatt ttaggaacaa agaggtgaca aaatggacaa
    13801 gaaatacgac caatgaacag tacaaaatct tgatttgaat caattttatc ttcaatttcg
    13861 gcagccgtta tcaaagtcat ggttgccaaa agttcttcaa aagaatacat agttaaccaa
    13921 ctttctaaat cttatattta aagaataacc tatgatggca aaacttgcaa ggcaagagta
    13981 acaacgattc tatcaaaatc attaactcac ttctcgcttc aaactatcct caaaaatgct
    14041 cactcatatt taaatgactc atcaaaaaaa ctgagcatag ctcagttttt attttacacc
    14101 ttatttttgt acagcattac taacgctttt agcaactaca tcggccacat cattttgaaa
    14161 agcatttgga atgatgtgag ttgtgcttaa ttcttcctcc ggtattaaac tagctatgcc
    14221 tctcgctgcc gcaatttgca tttcaacagt tatcgttttt gctctagcat ctaatgctcc
    14281 tctgaaaata cccggaaatg ctaaaacatt attaatttga tttggaaaat cactacgacc
    14341 agttccaact atgtaagctc cggcttttaa ggcttgatct ggataaattt ctggaatagg
    14401 attcgccatt gcaaatacaa taggtttatc agccatcttg gaaatccatt ctgcatgtaa
    14461 tgcttccgga gcagaaacac ctataaaaac atcggcattc tccaaagcat cttctaagga
    14521 tccagattgg aattctctat tagtcaattt tgcaatcgct ttgtgatgag gggctaatga
    14581 ctcccgatct ttatcgttaa taatgccaaa tcgatcaact accgttacat gttttgctcc
    14641 tgctgataga agcttccttg ttatagataa gcccgctgaa cctccaccat taacaactac
    14701 tttgatatct tcaatgtctt ttttaactaa cttcaaactg ttaaacaaag ccgctaatac
    14761 tacgattgct gtaccatgtt ggtcatcatg gaaaactggt atatcacatt cttcaattag
    14821 gcgttgttca atttcgaaac aacgtggtgc tgaaatatcc tctaaattaa tccctccaaa
    14881 agtcggcgaa atcgctttta ctatagaaat aatttcctct gtatcatttg ttttcaacac
    14941 aattggcaca gcatctacat ttgcaaaacg tttaaagagt gctgctttac cttccatgac
    15001 tggcattgct gcctcaggac aaatatctcc taatccaaga acagctgaac catcacttac
    15061 tacggctact gtattttttt tagtcgtcaa ctcatatgca agctccacat cctctgcaat
    15121 agccgatgat actgacgcca cccctggggt ataggcaata ctaagatccc tcttatcttc
    15181 caccttaact tttgaaatta cttctaattt tccaccattt tctctagctt ggttgatagc
    15241 taattgacct aaattttcac tcattttgat aactcctttt gataaatatt agaataacat
    15301 gcgtaaaatg gctgtcatcg tgataacagt aatagctcct cctaaacgcg tagcaacttg
    15361 agcaaatggc atcaagttca ttcggtctgc tgtacttaaa atggcaacat ctcctgttcc
    15421 tcccatacca ctttgacaag ctgaaataat acctgcttca acaggattca tattaaggaa
    15481 tcgcgataca aagaatccaa cagaaataac tgtaaataca acactaataa caactatgaa
    15541 atattgtatg ctaagcgttg ccacaacatc ttttaacggg atataaagca atcctagccc
    15601 tgccattaga ggaaatgtaa aattaccaga aataaattta taaagttgct tagctccatt
    15661 ttgtgtttct ctaggaataa cattgatata tttcaaaata gctgctgcga taatcattaa
    15721 tacgggacca ggaaatccag ttaattgctg caacaaatgt ccaaaaataa agagactaca
    15781 tgctgttaaa acacctccac ccattttctt aacgtcaatt gggccagaat gatcagcaat
    15841 aatatctgac atgtcctctc ctttatttaa ccttactaat tgcccttgac cagacaagtg
    15901 tggtttcttt tctcccaaac gattcaatag tgcagtacat aaaatggcaa agaaattacc
    15961 aataatagtg gctgggatga gttgagcaac tagttgttcg ctagctacac cggtaattga
    16021 actatagcct aacgataatg gtaaaatacc ttcaccaata ccaccagcta aaacgggtgt
    16081 tacgatataa aacaaagtgt gtcgccactc taagcctaaa ataacaccga caaatgtccc
    16141 taccatcatc gcacaaacca ttcctaataa catggggaaa atcattctta gtaatccctg
    16201 aatcaacatt ttccgattca tccctaaaat actaccggac actaaacaag caatataaaa
    16261 ataaagaaag ttagcttgct tcatcaacat atttgttgac tctaaaacat ttttattaat
    16321 gaggttgaaa aacaccaaaa tagatggtac taataaagat aaaatagctg ggccaccgaa
    16381 gtttttaaaa ccagggatgc tagctccaat agtacctaag aaccatccca ttgttaagat
    16441 gactgcaaat cctcctagca tattgactgg caatttttgt aaaaagcctg ctagaagaat
    16501 caataatgct aaacaaacat atactggtaa gggaactgag cctaccttaa tatgaaatag
    16561 tttagtcttt aagtcttgct tttgacctct agaaccctca ttattcacta ccttcacgtc
    16621 tgccatgaaa gcacctcctt ttacttctta tgtacagttt actttgggac ttagtgataa
    16681 ggaagtttat ttaagtaatt gaattatttc aacttattta attaatttaa gctggattct
    16741 agtttttagg ctatgttata ctaaactcgg aggatgctga tgaaaaaaaa actatctctt
    16801 tgggcatttc tatcattgat tttagtcact atgaccattt gtatattttc tattttttat
    16861 tatgtcacta tacatcagtc ctatcgtatg gtaagggtac aggaagaaaa aatcttaaaa
    16921 aatactggtt atgctttatc gagaaatcca caagttatac aaacactcaa agataatcac
    16981 tataatcaaa gtctccaaaa acaaatgcta ttccttagca aaaaatcaaa tttagactat
    17041 attgtcctta taaatttaaa agggatccgt tttacccacc ctgactccac taagattggt
    17101 aaaccatttc aaggcggtga tgaacaagct gtttttaagg gaaaagctat tatgtctact
    17161 gcagaaggtt ctttaggaaa atctttacga tacttaattc cagtatatga tcaccaaaaa
    17221 caggttgggg caattgcagt tggcttaaaa ttaacgacac ttggagactt atctcaaagc
    17281 agcataaaag agtttagtaa gcctctcttg attagtattt tgattagctt agtcgtcaca
    17341 agtataattt cttatggctt aaaaaaacaa cttcacaacc tccatccaag tgatattttt
    17401 caacatttag aagagcgcaa tgcaacactg gaccaaattc aagccgctgt atttgttatt
    17461 gaccagaggc atatcatcaa aagaaataat cctgcagcta gtttattatt caaaaaagag
    17521 gggcaacgag acctattttc aggtaaatta ttagaatctt taattcctca actcaagcag
    17581 gatcattttt caaaaaaaac tgaacaagta ttacattttc aaggacaaga ctatttacta
    17641 tctatctcgc caattacggt taaaacacaa aatagaggct atgttgtctt cttaaggaat
    17701 gttactgaaa cacttttcac tttggatcaa ttagcacaca ctacagctta tgcttctgct
    17761 ctacaggccc aaacacatca atttatgaat caacttcatg ttatttatgg acttgcagac
    17821 atcgaatact acgacgagtt gaaaatttat ttaaaagaat tattggagcc acaaaatgaa
    17881 tttttagcac gcttatcaat gttagttaga gagcctcgac tggctagttt cattattggc
    17941 gaaagagaaa aattcgcaga aaaacacatc aatctatcca cagaaatttt agtagaaatc
    18001 cctaccaagt caactgttga ggatgttaat aactatctct tgctccacag atatattaat
    18061 acgaagatac tcacgctatt aaattcaacg actttagttt ctttacgttt aaattatcaa
    18121 aataacctca ttgaaacaga ttatcaatgg gagaatgaga agtggttact caatgactac
    18181 catcagtatt tcaacgacgc ttattttcaa cagttacttg tagattcaag agctacttat
    18241 aagatctctt taaaacaaaa taaggttatt ataagaacaa atattatcta ctcagaaggg
    18301 tcttagtatg gacgtattaa ttatcgaaga tgatcctatg gtggaattta tccaccgcaa
    18361 ttaccttgaa aaactcaatt attttcaaaa catctattca actgctagtc aaacacaagc
    18421 tattgcttat cttaatgaca ttaaaatcca acttgttctt ttagatattc atattaaaga
    18481 aggcaatggt ctagaattat taaagttatt acgcaatcaa caccaaaata ccgaagtcat
    18541 tgttatttca gcagccaacg aggcgcacac tgtcaaagaa gcatttcatt taggtattgt
    18601 agattacctg ataaaaccat ttacatttga acgctttgag tctagtattg aaaaattcct
    18661 aaatcattat catacatttg aagctgataa aatctatcaa gacaacatcg atcactttca
    18721 aaaaatagat tctggatggc tagagggaga ggttaaactt gatgagaaag ggctctctga
    18781 aattacctat cagcatattc ttgatgccat tcaagagtta gagcagcctt tcactatcca
    18841 agagttagca aaatgcagtc aattttctca tgtctctgtt aggaaatata ttgcctatat
    18901 ggaagaaaaa ggccttctga caagtcagca aatttatact aaggtcggtc gtccttacaa
    18961 agtttataag cttataaaaa aaacttaggc ctgtagacct aagttttttt aatcttcgta
    19021 gccatttgga tgactagaat gccatgccca tgccgatgaa ataattttat caatattatc
    19081 aaatttaggc tcccacccaa gcatttgacg agctttctct gaggaagcaa taagcgtatc
    19141 tggatctcct ggacgacgag ctgccttttg agcaggaatt tctttcccag taatacgacg
    19201 agccgcttct aacatctgaa ggttagaaaa acctgtcgaa gatccgagat taaacacgtt
    19261 tgattcattc ccttggcgaa ggtaatcaac tgctaatata tgagcatctg ccaaatcaaa
    19321 tggatgcaca taatctcgaa cattagttcc atctggagta ttgtaatcat ctccaaaaat
    19381 cattattttg tcacgtactc cttgagcaac ttgaagaatg attggtaaca aatgtgtttc
    19441 tggtttatga tcttccccaa ttgagccatc cggtttatct ccagcaacgt tgaagtaacg
    19501 tagagcgaca aacttaatac cgtaggcttg atcagcccat ttcataattg tttccatcat
    19561 aagcttactt tcgccataag gattaatagg attttgagga gctgtttcta aaataggaac
    19621 ttgttctgga ataccatatg ttgcagctgt tgatgaaaag acaatatttt tgatatcaca
    19681 ttcattcatt acttctaaaa gttttatcat tccagcagtg ttattatcaa aatattttaa
    19741 aggattttcc attgactcag caactagtga aaatgccgca aaatggataa cagcatctac
    19801 atcaggattt tccctaaaaa cttgacgcat aaacgtttta tcggataaat ctccttcata
    19861 aaaaatcgca tctgaatgca ccgcttgacg atgacctgtt accaagttat caacaacaat
    19921 aactttctct tttccttgtg tgattaactg atcaaccata tgcgagccaa tgtaaccggc
    19981 gccacctaaa atcagaactg ccatattttt ctcctatttc aatactcttt taacatcatt
    20041 tgttacctca ttatactgag aataagcttt ttttgcaaat atctaccgaa attagaatag
    20101 ctctatgcag aaggcatccc acggcttgag agtgttactt gatagtactt cttgaacctc
    20161 tgtatttgaa ataatagttt ctttaacagc ttctggaaat tcaaaagatt ggttttgatc
    20221 ggataagttt gcaactatta aataacgttc tttatctgtt tgacgaacat aagcaaatac
    20281 cttatctgct gtttccaaaa gtttaaaatc agcatcaact aaccagtcgt gttctttacg
    20341 taaagcaact aatttttgat aagtataaaa aatactttcc gtatcattta acgctgcctc
    20401 cacattaatt tcctgataat tgggattgac tgctagccat ggggtaccgt tagtaaaacc
    20461 agcttgatgt ccttgactcc actgcattgg agtacgagca ttatcacgac cgactttacg
    20521 aatactatct aaaattgttg cttcagatac cccattatcc atggcttctt tagcatagtt
    20581 aagggattca atatcatcta catctgctaa acactcaaat ggataattgg tcataccaat
    20641 ttcttcacct tgatagatgt aaggcgttcc acgcatcaaa tgtagtagga ttgctagagc
    20701 cttggcagat tgttttctgt tatcactatc atttccccaa atcgagagca cccgaggaag
    20761 gtcgtgatta ttccagaata aagaattcca accttggcca agctccaatt gtgtttgcca
    20821 ttttgtaaaa atatctttca atgctggaac atctaatccg tcactgtaat cccacttagg
    20881 agcgtcaggt ttatgttgaa gacctacatg ttcaaattga aagaccatag ataattcttc
    20941 attatcggga tttgagtatt gttttgcaat gtctggcgtt gctccccaag tttcacccac
    21001 cgtcaataag tcatgctttc caaaactagc tctgttcatt tctttaagat aatcatggag
    21061 ttttggtcca ttaccagtaa tgcccttatc tggaattttc ccaattaaat caatgacatc
    21121 catacggaaa ccaccaatac ccttatcaat ccagaaattc atcatatcat aaattttatg
    21181 acgtaaagct tcattctccc agttgagatc tggctgacgt ttgctaaaaa ggtgcaaata
    21241 atattgacct gatgctttat catattccca agctgagcca ctaaaaatag aagtcaaatc
    21301 gttaggttca tctcgccaaa tataaaaatc acgttcagga ctattagggt tttctcgcgc
    21361 ttctacgaac caagcatgtt catcagaggt atgattcact accaaatcca tgatgatttt
    21421 aatcccgcgt tgatttgcct cttgtaacag ttggtccata tcattcatgt caccgaaaat
    21481 atctgcaatg gcttgataat ctgaaatatc ataaccattg tcatccattg gactttggta
    21541 aacaggtgat aaccagatag cagtaatccc taatttttca aggtagtcta gtttgctagt
    21601 aatcccttta atgtccccca caccatcacc atctgaatcc ataaatgaac gtgggtaaat
    21661 ttgataaatc gtagctttat gccaccagtg tttcttcatt attcttctcc tatcaataat
    21721 tcattcatct tttattttaa cgtttttcct cttttagtgc aagcgtttgc gcaaataaca
    21781 ttataaaaag gctggaaccc aaccttttta tgaatatcgc ttatacctta acgaatagct
    21841 tgttcagtat ctgcatcaaa gaaatgcccc ttagcaacat taaacgttag gttaactttt
    21901 tctcctggat tatggaagtc acgcgcttca acacgagaag caaattctgt ttgacctaac
    21961 ttaacataaa gcatagtttc gctaccgagt aattcagaaa ctaaaacttc tgcttcaaca
    22021 ttggcatttg gataagtatc ttggacaaga agattgcttg aaatgtcttc tggacgaata
    22081 ccaaaaatca attcttttcc tttatagcct cgtgattcta aaagtttttc ttgaccttca
    22141 gggatagcga ttcgaagacc atcttcacta ataatcatac cattttcaac tttaactttg
    22201 aagaagttca tagatggact accgatgaat cccgcaacaa atttattagc aggaagatta
    22261 tagagttctt gtggtgaacc aacttgctca attttaccaa ttgtaccatc accatcaggg
    22321 ttcttagtag ctgacataat gacaatacgg tctgccaaag tcatcgcctc tgtttggtcg
    22381 tgggtaacat aaatagttgt tgaaccaata cgttgatgaa tttttgcaat ttcagcacgc
    22441 attgaaacac gtaatttagc atctaagtta gataaaggct catccattaa gaacactttg
    22501 gcatcacgca caatagcacg tcccatagca acacgttggc gttgtccacc tgacaaatcc
    22561 gcaggtttgc gctctaagaa ttcagtaagg ccaatattag ctgctgcttc acgaacgcgc
    22621 ttatcaattt cttgttttga gaatttacgc aatttgagcc caaaagccat attatcgtaa
    22681 acagtcatat gtggataaag agcatagttt tggaaaacca tagcaatatc acgatcttta
    22741 ggtgatttgt cattgacaac ttcgccatca atcttgagct caccttcact aatatcttca
    22801 agacctgcga tcatacgaag tgttgttgat ttcccacaac ctgatggtcc aacaaaaaca
    22861 ataaattctt tgtctttaat atccaagtca aagtcttcaa cagaataatg cgatgcactt
    22921 ggatattttt tgtaaatatg atttaaattt aattctacca ttagattgac ctcttttatt
    22981 attgtgattt tattatagca aagaaagggt tttcatataa tggtaacctt gcctaaattt
    23041 cttgtgcaaa ctaactaaat tttaggtatc catcgaaatt aaataggcat aggctaaatc
    23101 atctaatatt tttaaattaa gccccgaaga ttgggtaaat ttatctaatt tatattgcaa
    23161 tgaattccga tgaatgaaaa gtgccttagc cgttttagca aggttccctt gctcttgcca
    23221 caaggctttt attatagccg aagtatcact cgtatcaagg atatgttgta aacattttgc
    23281 tttaatagaa ggcatgggcg attgatttgc caaagcccat aaagccatct ttggaaatcg
    23341 acagtaatgt tgttcggcct tgtgactaag aaaaagcgta aaaagtttat attcttcttc
    23401 atataattct ttccagtcca ctgcttgtag atgcgtccaa ctatttccaa aatagcatct
    23461 taaacgcgtg ttaaagtcat tttcaatcgt tggtaaaata tctgttagta attccgtagc
    23521 attaaagaaa ttatcctgat ttaaaatcat gacactttgg gtttgattaa ttgtcataat
    23581 tgtttctacg tttattacta aaggcgacaa gaaatcctta agttcttctg ataaggtaaa
    23641 ttgatgctca ataaaaataa attggactgc cgaataagat tggggcaacc ttccacgacc
    23701 ttcaactaga tagcggtacc aaggattctg agaatccact gaactcgcct cttccaggct
    23761 ttgtgtcatt tgtaataagc atagttcacg cttagttagc gaagattttg gaaagtggag
    23821 gacatgacca tcttgctcaa cgatgatata atcctcactc tgtttaactt cctgtttagt
    23881 aataacttct ggaaacagtt ccttaaaatc catggcatcc tccactacag ttttgttcat
    23941 tatatcatat ttataacctc tttttgagca cagaaaaaga ggagaatgtc tcctcagaaa
    24001 gtttctgtta taaaatagtg atccgaatag aatgctgttt cgactgcttt ggattaagcc
    24061 gtacgatatt ttgtttatct tctaaaatat cagactcttc aatagaagtt gacaaggaac
    24121 tccagggttc caaagccaaa aaaggaccac cattgttact tgtccaaaga ataagattct
    24181 caaaatcatc aaagtcaagt tggattcctt ttgcatgatt tctagatttt aagtagaccg
    24241 tcttagatcg tagttggtct aaagtaatag catctttttc aaataaagca taattaagag
    24301 ataattgttt ttgattttct aaaaaaggat gtctagcttg taaatctaac aagcctgtat
    24361 ccggaaacga ctcaggaatt gtgcaagttt caaactgttc aaactctaga tagtaatcac
    24421 taaatacttc cttttcaaac aaagggcaat taaagcctgg gtgagctccg atagcatatg
    24481 gcatattttt ttctgattct aagtttttga caatatatgt aatggcaatg cttttatttc
    24541 ttaaggtgta agttatttct aaactaaaac gataaggata atttatcaac gtttcttcac
    24601 tacttttaat tgtaaaacga acagaagaat ccgatatgta atcatagtcg aatgttttgt
    24661 gacgtacaaa gccatgcctt ggtatgatac ccgtaaaatg tggagcttga gctggtctgt
    24721 aaatgacgca atcatttcga acacttccgc atataggaaa taaaatagga gcttgcccac
    24781 cccaataagt gggttcccct tgccaaaggt actcaacgcc ttgactatct gtaatagaag
    24841 tcattgttgc acctaacgct tttaatgtca cttgcaactc atgattttgt atcgtaatag
    24901 ccattatttt tcctttcata aagagcaata attgctttac caacttttct ctagcaatta
    24961 tctttccttc caagaagttg ctgttttttg caaaatttta tttagttcat caatattttg
    25021 aaaacctatt gtacgtaacc attgacgtgc accagcttct cctttttcaa catactcttt
    25081 aacacttcca gcccaagttg ctctaccaca aaggacaccg ttaaatttag caccagcttc
    25141 tttagcaaac acaagagtct cttgaaaaag ctgagcagga actcctgcac tcaaaaaaat
    25201 gtaggggagt agtgtcgcct gatcttgttc tctaaaataa gccgcagctg tcgctttatt
    25261 ataagcagtc tctccttgcg caaaaccttc tacatagtcc atattgacag gcacttccac
    25321 cttcaagaca tcaatattaa atcgaggatt tgaaaaaact ttcatagctt caataacctt
    25381 acgtggctta attttggcat actcaatacc tgagctatcc gtaatctttt catcatatga
    25441 caaaatttca aggaaaaaag gaatgtcttc tgcaacacat tcagaaccaa tccgttcaat
    25501 ataagcttct ttttcttcgt ttacttcatc cgaactatca acatcataat aaagtaagaa
    25561 cttaactgcg tctgccccct cttctttaat gcgtttcgca gaccaaatgt ctaaacagtc
    25621 tgggagtcgc ttggtactac tggtatcata acctgttttt tcgtacgcaa gtaaaagacc
    25681 ggcattgtca tctaaaactt tcgtagctgg caacccatat tctggatcta acaacattga
    25741 tgaagcgtaa ggggttaatt cttcagctac caaaacttta agtgcttcta tttgagaaac
    25801 agtgggctcc tcagattgat attttgccat taatcgcttc aatgctccac gttggtcaaa
    25861 agccaaggcc gaaataatac catttttatc actcaattgt tccatatgtt tttgtttttg
    25921 ttccgttaat cccatcctat acctctttca cttggatttg actgattaat tcttgatagt
    25981 aggtcagatt gatatgccct gttaatgttt cttgtgcatt aagcataccc aagacattag
    26041 ctctcttaag taaatttata tcatctagtt gatgttctaa agcagacgcg attcctgcaa
    26101 ccgttgcatc ccctgaacca actggattga taacttctat ttttggaatc gtcacttgat
    26161 agtagtgatt cccatgttta gcaaaagctc ccctagcacc taaggaaaca ataacccaat
    26221 caattcctct aaaaatatct tgctgtaagg tagattttaa ttcgtctaaa ctatagctaa
    26281 ttggccttcc aattaattgt gttaattctt caaggttagg tttaatgacg gtcggcttag
    26341 cactagattt taaaaccgaa cgtaatgctt ctcctgaaca gtctaaaaca actttcttat
    26401 taaagtgatt cgctagggag attaacctag cataataatc tgaggggagt ccctttggta
    26461 aacttcctga tactgttatg atagttgcag catcaaagat aagttttaga tgattcaaaa
    26521 aggcgcctgc ttcatcttta gtaatatgtg gacctttttc tagaatttca gtctgctgac
    26581 cctcatgtaa aactgcaata caatgacgtg tttctccacc aatacttaaa aattggtgtc
    26641 tgatttccaa tgcatctaag ccacttcgga tgaaatctcc aaaatgcccc cctaatagac
    26701 cagttgccac tacattatcg ccaagttgag aaagaacacg agtcacattt aatcccttac
    26761 caccaggtgt tttggagacg tcggtaaccc
//
LOCUS       DATRME010000020        26548 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRME010000020 DATRME010000000
VERSION     DATRME010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26548
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..785
                     /locus_tag="VBC23_000853"
     CDS             <1..785
                     /locus_tag="VBC23_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842818.1"
                     /translation="KINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNSTYILIVLF
                     TLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILAYALILTVF
                     ADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGLLFIASLNF
                     FILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTSALLLKLSR
                     NGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            1014..2048
                     /locus_tag="VBC23_000854"
     CDS             1014..2048
                     /locus_tag="VBC23_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6842819.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            2295..2720
                     /locus_tag="VBC23_000855"
     CDS             2295..2720
                     /locus_tag="VBC23_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6842820.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            2738..3241
                     /locus_tag="VBC23_000856"
     CDS             2738..3241
                     /locus_tag="VBC23_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6842821.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            3245..4054
                     /locus_tag="VBC23_000857"
     CDS             3245..4054
                     /locus_tag="VBC23_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6842822.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4051..4878
                     /locus_tag="VBC23_000858"
     CDS             4051..4878
                     /locus_tag="VBC23_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6842823.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            5016..6665
                     /locus_tag="VBC23_000859"
     CDS             5016..6665
                     /locus_tag="VBC23_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6842824.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            6669..7442
                     /locus_tag="VBC23_000860"
     CDS             6669..7442
                     /locus_tag="VBC23_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6842825.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            7454..8491
                     /locus_tag="VBC23_000861"
     CDS             7454..8491
                     /locus_tag="VBC23_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6842826.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(8713..9210)
                     /locus_tag="VBC23_000862"
     CDS             complement(8713..9210)
                     /locus_tag="VBC23_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6842827.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(9210..10247)
                     /locus_tag="VBC23_000863"
     CDS             complement(9210..10247)
                     /locus_tag="VBC23_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6842828.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGSYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            complement(10258..10713)
                     /gene="nrdI"
                     /locus_tag="VBC23_000864"
     CDS             complement(10258..10713)
                     /gene="nrdI"
                     /locus_tag="VBC23_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6842829.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(10870..13272)
                     /locus_tag="VBC23_000865"
     CDS             complement(10870..13272)
                     /locus_tag="VBC23_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6842830.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQKEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            13532..15748
                     /locus_tag="VBC23_000866"
     CDS             13532..15748
                     /locus_tag="VBC23_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6842831.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            15758..16201
                     /locus_tag="VBC23_000867"
     CDS             15758..16201
                     /locus_tag="VBC23_000867"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6842832.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            16456..17379
                     /locus_tag="VBC23_000868"
     CDS             16456..17379
                     /locus_tag="VBC23_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6842833.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            17406..19988
                     /locus_tag="VBC23_000869"
     CDS             17406..19988
                     /locus_tag="VBC23_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6842834.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPIKKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(20269..21045)
                     /locus_tag="VBC23_000870"
     CDS             complement(20269..21045)
                     /locus_tag="VBC23_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6842835.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            21255..21728
                     /locus_tag="VBC23_000871"
     CDS             21255..21728
                     /locus_tag="VBC23_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6842836.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            21730..22035
                     /locus_tag="VBC23_000872"
     CDS             21730..22035
                     /locus_tag="VBC23_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6842837.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            22075..22562
                     /locus_tag="VBC23_000873"
                     /pseudo
     CDS             22075..22562
                     /locus_tag="VBC23_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
     gene            22803..25253
                     /locus_tag="VBC23_000874"
     CDS             22803..25253
                     /locus_tag="VBC23_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO6842838.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSTEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAGEDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAMQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTQKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEIHDAYVQLSAIRFEHDKKEYILLVNANGPGKKCQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
     gene            25503..25927
                     /gene="lacA"
                     /locus_tag="VBC23_000875"
                     /pseudo
     CDS             25503..25927
                     /gene="lacA"
                     /locus_tag="VBC23_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            25948..26463
                     /gene="lacB"
                     /locus_tag="VBC23_000876"
     CDS             25948..26463
                     /gene="lacB"
                     /locus_tag="VBC23_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6842839.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
BASE COUNT         8687 a         4213 c         5096 g         8552 t
ORIGIN      
        1 ataaaataaa tattaaagat caaaaacagt tgggttctgc gctaccacta aaacaactaa
       61 agctaggtaa ctttgctcct tggaatcaac tttttgaagt aagtaatagt acttatattt
      121 taattgtctt atttactcta ttcttatcag ctcctctttt ttcaggagat agggctaaga
      181 agattaatcc tatactactg acgactcggt atggacgtgg tcagttgacc tgggctaagt
      241 tagctagtac aatgaccatc ggtatttcca catttatcct agcatatgcc ctcattctga
      301 cggtatttgc agattatttt ggttttagtg gttgggagac aagtgttcaa ctgaatttat
      361 actggacatc acctttggct aatattatgg gatttcctgt acctatgaca atatcaactg
      421 cttttgttca tattattgtc ttacaatttg ttggactcct ttttatagcc agtcttaatt
      481 tctttatatt aagtatcaca aattcgccat taacatcttt tgctgtttca acgcttgcct
      541 tttttgctcc tagcttttta atggctatgt ttaataaagg tgtgataaac aagttattaa
      601 ctattttctc agtatcaacg acagacactt cagctttact tttaaaatta tcacgtaatg
      661 gtagtcatgg ctttttcttt gatagttttt ctgctaatga aatctgtctg attagtctac
      721 gtatgttaat tgcagtgtta ttgatagctt atacctatcg tttaatgcga cgaagaagtt
      781 tatagttccc actggttatc tgaacaagaa attattctaa gctgattttc ttttgcgaaa
      841 tcagcttttt tatttcctaa tatggttgat aaggtattaa caataattat cagataccat
      901 ttaaggcgtt tcaatgactt ttggggataa ttgagatatt cttctgaaga gattggtatc
      961 atagtaatga taacttatag aaagtaatgt taaggatgaa gagaagtcag tgcatggata
     1021 ataagcagca taatacatca atgattagct tgctacaata tcttttttcc atactggtca
     1081 ttttagttca tagtggaagg ttatttagtc aggatgtcat tcattttacc tttaaaagtt
     1141 tcctaggtcg gatggctgtt ccttattttt tgatttgtac tgctttcttt ttgagaggtc
     1201 gtatacagca aggtttgtgt aatcactcct actttcgtaa actgattaaa aagtatagca
     1261 tgtggactat catttatttg ccttatggtt atttcttttt tgaatctcta aatatagcga
     1321 agatttattt gctgccgggt tttattgtgg catttttgta tttaggtatg tcacatactc
     1381 tttggtacat ccctgctgtt attctaggtt ggttcattat tcaaggatta ttgaagtatg
     1441 taggaactag ggggacattt atcacagttg ttgtactcta ttgtattgga gcagtagaga
     1501 cctattctgt ctttgtacag tcaacaaaat tttatcccct gatgtccact tatatgtcaa
     1561 tttttcagac aacacgaaac ggattatttt acactccagt ttatcttcta gcaggatatt
     1621 tactctatga ttattttaat actgacctat ttaccaaatc gcgtggtctt aagtatattc
     1681 tcttcctttt acttctagct ttagagaatg ttcttattta ttttaatcaa gggttggata
     1741 aaaatttctt cttattagct cctttatgtg cagtattctt atttaactgg agtatacgta
     1801 catcactgtt taagggctat cgtttaagtc ctttaaaaca gttgagtgtc tattatttct
     1861 tccttcaccc tctttttgtt ggcatagtgt cttactgttt aaaatcaact tccttagttg
     1921 cacaccatca aggaaaagtg atttttgtgg tgaccttggc attaacacat gcttgtgctc
     1981 tactcataat aaaggttgtt aataagatac cctatcaaaa aattattcca ttatctcttt
     2041 cgaaatagtg attcatataa gagaaagttt aaagttatag tgatgtaaag gttgtgtaaa
     2101 ctattaggat acatgacctt tttattgttc tgtttttaaa tattaattat atagaatagt
     2161 ccacacatag agtagaatag tccattttta aaccgttttc atttgttaga atgatagtaa
     2221 aggttatctt attcaaagta ttgataaaac tttcagaact aatttagggt aatgtaataa
     2281 ataggaggag aaagatgaag cggaaattcc taatcggcag tcacggaaaa cttgcgagtg
     2341 ggttgcagag ttctattgat attctgacag gcaagggaca agagattcag acaatagatg
     2401 cttacattga tgatagtgac tataccaagt ctattgtaga atttattgat gagattgcac
     2461 cagatgaaca ggggcttatt tttacagatt tgttaggtgg cagcgttaat cagaaaatgg
     2521 cgactgctgt aatgaatagt gggaaaaata atgttttttt aataacgaac agcaatttag
     2581 cgaccttgtt gtcattacta tttttgaaac ctgaagagga attaactaaa gaagaaattg
     2641 taaccgttat caatgaatct caagtgcagc tggtagatct cagctttaaa gcgtgttcag
     2701 aagatgactt tttcgattaa taattaggag gaaaaaaatg attactcaaa ttcgtgttga
     2761 cgaccgtttg attcacgggc aagtggcagt tgtatggact aaagaattaa atgcaccact
     2821 tttagtggtg gctaatgatg aggctgccaa aaatgaaatc acacagatga ctcttaaaat
     2881 ggctgtgcct aatggcatga aattattaat ccgttcagtt gaagaatcaa tagctctatt
     2941 taaagatcca cgcgcaactg acaagcgcat ttttgtgatt gtcaattctg ttaaagatgc
     3001 ctgtacaatt gctaaaaata taaccgattt agaagcagta aacgtcgcta atgtaggacg
     3061 ctttgataag tctgatccag ctacaaaagt caaactaaca tcaagtctct tattaaatcc
     3121 agaagagttg gaagctgcaa aagaattagc cagcttacca gatttagacg tctttaacca
     3181 agtattgcct tcaaatacta aggttaactt gagtcagcta gtcaaacaaa gtgaggtatg
     3241 acatatgata atgccagcga cgatggcagc tttagctgtg ttgatttgtt ttgggggaaa
     3301 ttacttaaca ggtcaaagta tgatggagag accacttgta gttggtcttg taacaggaat
     3361 gctactaggt gatattaagg ttggtatctt aatgggtgct tccctagaag cgctattttt
     3421 gggaaacgtt aatattgggg gtgttattgc cgcagagcca gtaacagcaa cagcgatggc
     3481 aacaactttt accattattt caaacattga tcaaaaagca gcaatgactc tagctgttcc
     3541 gattggaatg ttggcagcat ttgttgttat gtttttgaaa aacgtcttca tgaatatctt
     3601 tgcaccgatg gtggataaag ccgcagccgc aaaccatcaa ggtaaacttg tgatgcttca
     3661 ctatggtaca tggattattt actatctcat tatcgcttct atttctttca ttggtatttt
     3721 agtaggtagt gggcctgtta atagttttgt ccatcatatt cctcaaaacc tgatgaatgg
     3781 cttgagtgcg gcgggaggcc tccttccagc tgttggtttt gccatgctga tgaaattatt
     3841 atggacaaat aaattagctg ttttttactt gcttggtttt gttttaacag cttatttaaa
     3901 attaccagcg gttgcagttg ctgccctagg agcagttatt tgcgtcatta gttcacaacg
     3961 tgatatagaa ttagacgcaa ttactagagg tgcgatcagt aaacagacaa cctttgactc
     4021 taaagaatca gaagaggagg acttcttcgc atgaaatcac aggataattt gactaaagaa
     4081 gaccgtaaaa tgcttcgctc agtcttctgg cgctcatgga cgatgaatgc cagtcgtact
     4141 ggggcaacac aataccacgc agtaggagtt atttatactt tactaccagt catcaatcgt
     4201 ttttataaga ctgataagga taaagcagag gcactggtcc gtcatacaac atggtttaat
     4261 gccaccatgc acattaataa ttttattatg ggtcttgttg catctatgga aaagaaaaac
     4321 agtgaagatc ctgattttga cgctagtgct atcaccgcag ttaaggcttc tttgatggga
     4381 cctatatctg gtgttggtga ttcattcttt tggggaattc taagggttat tgcggcaggt
     4441 atcggtatct ccttagcaag tacaggctca gccatgggag ctgttgtctt tctactcttg
     4501 tataacattc cagcttttct gattcattat tatagtctct atggaggtta ctctgtaggt
     4561 gcagggttta tcaaaaaatt gtatgaatca ggtggtatta aaattgttac taagacttca
     4621 agtatgcttg gtttaatgat ggtaggctca atgactgctt caaatgttaa attcaagaca
     4681 atcttaacgg tagcagctaa aggagctaag gaagcagctt ccatacaaag ttaccttgac
     4741 caattatttg ttggcgtagt gccactgttg gtaacgatat tagccttctg gcttctacgt
     4801 aaaaaagtca atatcaactg gatcatgttt ggtattatgg tgttaggtat tgtacttggt
     4861 ctcttaggta tttgctaata atctcctagc attaaaggta agtggaacaa gacgatactt
     4921 gtttagctta tctttttttc ttttgaaagt tattgtatga aaggagttga ctatctttat
     4981 tggatagcta tttgctataa taaaaggaga tggaaatgag aggatataga atggaagaac
     5041 gctttaagaa acgactacaa gatgatatct ctaaacattt ttcccgccag tctttaattt
     5101 tatccttgtt attgattgca cttttcgtcc ttttttcttt agcgcctcag cagataggcc
     5161 tttataaaga tgttaattca gtttcctata gttataagca attgattcaa aaacatgata
     5221 ccttattaga tgacttaggt aaaaatagtt tgaaaccatt tgtctcagga cacttaggaa
     5281 gtgctgattt gagtaagcaa tattatcatt taaggaatca tttacaaagt caaacagaac
     5341 ttttagtatt ctcacctaat caagaacttt tgtttgctag taattcacat ttaggtaatt
     5401 tttttagtaa gtctatttac attagtgaag ttttagataa ggctaaaata aatcaaaggc
     5461 tactaaaaat tattgtagat agtgagggag ggcattacct tgcactcatt aagccaatta
     5521 tagttaataa aaaagtttca ggttatgcct ttttattgat gaatgggaaa gactttttac
     5581 ttccaacgaa ggctattaac tcagacttga ttattgccga tcagctaaat aatagcttta
     5641 cttttactaa tcgtgatttt atctcctcta gtttagataa ggtagatagt caattcttaa
     5701 ctagatattt tagttttcac gatcatcgag cttttgttgt taggaaagta gctctgcaag
     5761 ataatatcct actttatatg tatcgtcccc tgataccagt aactctggtc gtcctttttt
     5821 ctctagtatc atcagttatt atttttgtga ttttacgacg aaaatctaga gttctagccg
     5881 atcgcatcgc agtaaaaaat tctggcgcca taaatcaaat ggtgctagat atggatgcca
     5941 tttcccgcca agaaaaatct agtattgagc ttgatagtca agatgaattt caatatctat
     6001 ctgttcaaat caatcagatg gtttcacggt taaaagattt acatgagaaa actctcgatt
     6061 tggaaacaca aaagttgctg tttgaaaaga ggatgttaga agctcagttt aatcctcact
     6121 tcctttataa tacccttgag acaattttaa taaccagtca ctatgattca caattaacag
     6181 aaagaatcgt tatacaacta acaaaattat tgcgctatag ccttagtggt agcacagagg
     6241 cagctgtgct taaggatgat ttagcaatca ttgaatccta ccttttgatt aatcaagtga
     6301 gatttgaaga attaacttac actatttcag tatctcctga acttgaacac atgcgtgtac
     6361 caaaactttt cttgttaccg ctaattgaga atgctattaa atatggctta aaagaacgtc
     6421 atgatgtagc aataaacata gatatttggc aagatagtga tggaatttgg tttactgttt
     6481 caaataatgg atcgggtatt agtttggcta ggcaaaaagc tatacgtaca atgttaagat
     6541 caaatcactc gcatcatggg cttattaatt cttatagacg attacaatat caattttcaa
     6601 cagtattgtt agagtttacg aagacagatg atgcttttcg agttagctat atagtaaagg
     6661 agtgagttat gtatagatta cttatcgttg aggatgagca ccttattcgt aagtggttgc
     6721 gctatgctat tgattatcaa tcattgaata ttttagtggt tggtgaagca aaagatggta
     6781 aagaaggagc tcaattaatt caggaagagc agccagacat tgttttaagt gatattaata
     6841 tgccaattat gacagcattc gatatgtttg aggcaaccaa aggacaatct tatgccaaaa
     6901 ttattttatc tggctatgct gattttccaa atgcgcaatc agctattcac tatggcgttc
     6961 ttgaattttt aacaaaacca ctagaaaagc aagctttgat tgattgttta aaaacaatta
     7021 tggctagaat tgaggaacat aaggagaagc acctacaaga gcatacagaa ttgtatttac
     7081 ctctaccaca agctaatgac caagttcctg aagttattaa agatatgcta gcatggattc
     7141 atagccattt tcatgggaaa attgtgatta gccagttagc tcacgattta ggttacagtg
     7201 aaagttatct ttacacagtc actaaaaagc accttcatat aactttgagt gattatataa
     7261 atcaatatcg tattaatcaa gccattcagt tgatgtttag agaaccagac ctaatggttt
     7321 accaaattgc agaagctgtt ggtatttatg attatcgtta ttttgatagg gtttttaaaa
     7381 agtacttggg tcagacagta aaagcgttta aggaagaaca tatttttaaa cagatggatt
     7441 aggtaggtga aagatgaaag aaaaacagtc gaaaaggctt atttatatac tactgattgt
     7501 tcccattatc tttataagtg tttttacata cagtattagc cagccttcta aactacttcc
     7561 accaaaagaa ttagttattc taagtccaaa tagtcaagcc attttaacag gaacgattcc
     7621 agcttttgag gaaaaatacg gtataaaagt taagcttatt caaggtggga cagggcaact
     7681 aatagataga ttaagtaagg agggtaagca gttgaaggcg gatattttct ttggaggaaa
     7741 ttatacgcaa tttgaaagtc ataaggcatt gtttgagtct tacgtatcaa agaatgttca
     7801 tactgttatt ccagactata tccatccgag tgatacggcg acaccttata ctataaatgg
     7861 gagtgtcttg attgtaaata acgaattagc taagggactt accatcaaga gttatgaaga
     7921 tttattacag ccttccttaa aaggtaaaat tgcctttgca gatccgaata cttcctctag
     7981 tgctttctca caactcacta atatactctt ggccaagggt ggttacacca atccaaaagc
     8041 gtggaactat gttaaaaagc tacaacataa tattaatgct atcaaatctt ctagctcttc
     8101 agaagtttat caatcagttg cagaaggaaa aatgattgtg gggttgactt acgaagaccc
     8161 tagtgtcaat ttgcaaaaaa gtggtgccaa tgtttctatt gtatacccga cagaagggac
     8221 agtttttgtc ccatcttcgg ttgcaattat aaagaatgct ccttctatga aagaagcaaa
     8281 gttatttatt aattttatgc tttctttaga tgttcaaaat gcctttgggc agtcaacgag
     8341 taaccgacct attcgtaaag atgcccaaac aagtaatggc atgaaagctt taaaggatat
     8401 cgctactctt aaagaagatt atcgctatgt cactaagcat aagagccaaa tccttaaaac
     8461 ctataatcgc attcgtagaa atgctgatta aatgaaaata tagaaaaact cctcggaatc
     8521 attgattccg aggagtttaa tcatcgtcct cgtcgctttc gaaatctttt ttccaataac
     8581 gataaagact aatgcatgcc agaaatccgg ctataatagg aacgataata aataagaggt
     8641 caatcactgt atccatgggc aatatactcc aatgataaga agtcattatt gtttatagtg
     8701 cgtacaataa tgttaaggta aaacgtcggt cattataagt tttttgacat cctctaaact
     8761 catagatttt gttaatattt tttcaggtat ctcttttaag aagtctttct caagccacca
     8821 ccttgcaaga gaatcttctc caaattctaa agatttaagt cgagttgaat gtcttttaac
     8881 agtttcttga aagcttaaat catagtaata agcataagta ttgttggggt tatgttttaa
     8941 aatatgtttc caaacaggcg tataccaatc agaacgaagt atgccttcaa ggatgatgta
     9001 ggaacagtta tggtaaccat aatttagtaa attgatcaaa aggggaatgg tagtagtatt
     9061 ttcaccatcc ttagttccta acatctctct acgaaggtaa tcctgagata agagaagggt
     9121 atcttcccct aattcggctt gaagttgttt agctattgta gactttccgc tagctgaatt
     9181 tcccctgata ataatgagcg tggctaccat tagttcacca tattgctttt gagataggca
     9241 tccactaaga gttcagttgc ttgaatagaa tcgatatggg tacgttcata agaatgacta
     9301 gattcaatac ctgcaccaag tagcgcgtgt ttaacttccg caccagcacg catggcagcg
     9361 gaggcgtcac taccataata aggataaata tcaagtttat aaggaatatt attgttttca
     9421 gctagagaaa caagatgttg acgtaattca taatgataag aaccagaagc atctttaaca
     9481 cagatagaga cagtatattc gtcagtttct tgatcgtctc ccatagctcc catatcaact
     9541 gctagatatt ctacagtttc atttgggatg cttgaatttg ctccatgtcc tagctcttca
     9601 aaggcactaa agtagaaatg agtagtataa ggaagttgaa tgtcttcttt cttgtaaaca
     9661 gaaagaagtt ccattaggat accagcggat accttgtcat ctaggtaacg tgatttaata
     9721 aagccgctgc ctgttatgat tgtacgcgga tcaaatgaaa tgaaatcacc gacctggatg
     9781 cctaaagcac gtgtttcgtc agcagttgtt actttctcat ctaaacgaat ttccatattt
     9841 gtttgattac gttcagcagt tccagcatct ttgtaaacat ggacactagt ttgatgaata
     9901 agagcagttc cagaaatttc tttaccattt tttgagaggt gtatagtaca gttttctcct
     9961 tcaatggcat tgtatgtgta tccgccaata aggtcaattt ttaaccgacc atctggtttg
    10021 atggccctaa ccatagcacc aagtgtatca aggtgagcag ttatcatgcg atgtttagta
    10081 ttattttttc cttttagaga aaccatgacg cctcccttgt ttgtgcgaat tggtgagtag
    10141 ccaaacgctt ccaattcttt gatgatatag gtcatgattg tttgggtaaa ccctgttggt
    10201 gaagggattg atgtcagggt tttaatgtag tttaatgttg tttccatact atttccttta
    10261 ggctttaaag ttcgataatg tttcaacgat aatatttgcg atacgttcaa cgtcgctaga
    10321 agttcctcgc aattcaaagt ctcctaacat aggaaatcca aaccgttcag agtattgttt
    10381 tgcagttaaa cagtactgat tgttaaaatt tctatttcca gagccaacaa ttccgataca
    10441 atttttgaca ttattgtgtg cagctataaa atcaccaagt ggaatggtaa gaatttcttg
    10501 atcgccattg tcaatgccat ttcctccctc gaggtaagtg ggtaatagtg ctacgaaagg
    10561 ttggtcaaca ggaaaggttt gatgattttg ttccttgata ttaatagtcg aaacctgaca
    10621 gttatgccaa gttgacagtt gttcactaag acgtttgaca aagctttgag tattgccgct
    10681 aagactgata tagactaggg ttaatttagg catttagtac tccttaaatc taaatttctt
    10741 gttatcagtt tagcaaaatc atgtcagata aacaagctag agtggtatct caatatctga
    10801 ctgtttagag agaaaatcta ttgttgagac taaaaactga ggttgattgg cctcagtttt
    10861 tagtgatgac tatatttgtt tctttttaag taaatttccc acgagtccaa gtattgcgac
    10921 gccaaagaaa gccagataag attgtttttc ttgtccagtt ttaggtaatg ttttttcttt
    10981 ctgttgtgta tagatagtgc ctgcgatatt tggagaagct tttgcttttg tataagcagt
    11041 tgcaaccatg tggtgaattt tttgtttctc tttttggctt aacttttctt gatgtgatgg
    11101 ctcagtaagg tctacttttt cttgttcagt atagacaaat cgataatcac caaatccttc
    11161 atctttggta gaactagcga tatactgaat atccttgcta ttagtaacaa gatttttagc
    11221 tttatcggct gtttggaatc gtaaatcaag gcctttaata gactctgtga agccccagtt
    11281 attatcagct gtaggattga tggttttctc tgaaataata tagtttataa tagtttggcg
    11341 attttctaag ttcagcaaac ggttcattgt ggcattttta gccccaggga aggtaccact
    11401 agcacggtag ttattggtca caatcataaa ctcttgtttg tctgcaacag gcttaccatt
    11461 gtacattaaa tcgcgtactc gactagcttg actattaaca acttttccat ctttgttgta
    11521 tttattaggt tgtgtaatat caaatttata agtcaagcca tcaataacgt caaaattata
    11581 ggtacgatat tcggtattga taatattttg cggtgcttta ttgttagggt caatttggtt
    11641 aaattgacca gcggacattt ctaaccattc acgtaagtct gctccggtca cttttaaaag
    11701 tgcagtaaca ttatcataaa ggtaaagatc agctacgttt ttaattgcaa caggtccagc
    11761 tggaatatca gtatatgcgg tagcatcgcc acgtgttccg gctttaaatg gtgctgcagc
    11821 agatagaagt ggtaaatttg cctcaggtgt tccttttaat tcatttgcaa cgtaccaacg
    11881 ttgagcgttg ttaacaattt gaactgaagg gtcatcttta actagtgcaa agtaacttgt
    11941 aattggcgca gttgttgtac ctacttgttg gcgaacatag ttgatagtgc catcgtgtgc
    12001 ttccttagcc aatgcaatga tacgctcgtc agcagttgtt gagttcatat caattttacg
    12061 gattttagca ctgctttcgg agacttgcca ttttccatta gtataactaa gtcctaaatc
    12121 aataatacca aggtgatccc cgtacttgcc tgccattgta acaggtgttc catttatttt
    12181 tccattgata ccatcaactc cagtgtattt ttcatagaag ccagtaccgt tacctgatgg
    12241 aaattcagcg tgtgagtgtc ccgtaacaac ggcatccact cccttgatgc tggcaatttg
    12301 atagccaacg ttttcttcac cttcttcata tcgatcatct ccaatgccag agtgtgataa
    12361 aacgagaatg acatctgctc ctttagcacg catagtagga acgatttctt cgattgcttt
    12421 aactgaatct ttgacaataa cctttccctc taagttagcc ttgtcccagt ttaagatttg
    12481 aggaggtaca attcctgtaa ttccaatatt tagtgcaaca gctcttcctg ttgaatcttt
    12541 gaaggtttta gtaatgatgt cataagtttt aaaaactggt tgatgtgttt tgaaatccaa
    12601 aacatttgcg ttgaggattg gaaggttggc tgtagcaatg atttttttga ggtaatctaa
    12661 accgtaatta aattcatgat tacctagtgt tgatgcatca taaccaagag cattcatggc
    12721 ttggtacata ggatgttctt cattttctgc gacaggttta acgattgctt tataagttcc
    12781 taaaggtgtc ccttgaatga catctccgtt atcaactaag acggtattag gattttcttt
    12841 ttttgcttct tcgataagga cagcagtttt tgctaaccct attttttgag attctttatc
    12901 ttgataataa tcatagttaa ctaaattggt gtgtaggtct gtggttgaca tgatgcgaag
    12961 gtcaactttt tcaccttcaa taggtttgag atcaccagtt gtggtttctg tggcaacagg
    13021 ctgtggattt tgattattgg cagtggtatt tgtgcttgtg gctgttgaag catcagttat
    13081 tgcagttgga gtagaagtaa cgtttgactt ttcctctgca attgtgctag tgggggtagt
    13141 tgattcaacc tttgtagcgg ttgaacttgg tgttgtaaca atgtcttctg cttgaacagc
    13201 taaaccactt gttgttgtag ctgttaagac agttaaagcg aatatgcttt ttgaaaaata
    13261 atgttttgac ataaatctcc tccattaaaa cattacatgt atattatcct aatctaaaaa
    13321 ctattatagc ttattgaaaa cgtttaatca atctcattta cgaattattt ttagtaatta
    13381 cactaaaaac aatcgtttat ttataaagga taacttggtt agacacattt acttggtatt
    13441 taattctttt tgagcctcta acttctttta tttttaggtt aaaaagtgat aaaataggta
    13501 caatctatca ctatgattaa agaggataac tatggtcaaa gaaattaatt taacaggaga
    13561 agaggttgtt gcaatcacat cccagtacat gagtgagacg gatgttgctt ttgtaaaatt
    13621 tgccttaaat tacgcaaccg cagctcatta ttatcaagca agaaagtcgg gcgagcccta
    13681 cattattcat ccaattcaag tagcaggtat tcttgcagat ttacaccttg atgccgttac
    13741 ggtagcttgt ggttttttac atgatgttgt agaagatacg gaaattaccc ttgatgagat
    13801 tgaaactgat tttggcaagg atgttcgcga tattattgac ggtgtaacaa aattgggtaa
    13861 agtggagtac aaatctcatg aagaacagtt agcagaaaat caccgtaaaa tgttaatggc
    13921 tatgtctaag gatatccgtg ttattttggt taagctcgct gatcgcttac ataatatgag
    13981 aacccttaaa cacctcagaa aagataaaca agagcgtatt tcacgtgaga ctatggaaat
    14041 atacgctcct ttagcgcatc gtttggggat tagtcgtatc aagtgggaat tagaagattt
    14101 atcttttcgt tacttgaatg agacggaatt ttataagatt tctcatatga tgagtgaaaa
    14161 acgtcgtgaa cgcgaagagt tggttgatat aattgtcgat aaaatcagat cctatactga
    14221 ggaacagggt ttatatggtg atatttatgg tagaccaaaa cacatttatt ctatttacag
    14281 aaaaatgcgt gataaaaaga aacgctttga tcagatttat gatttaattg cgatacgctg
    14341 tattatggaa actgctagtg atgtttatgc catggtgggg tatatccatg agttatggcg
    14401 tccgatgcca ggaaggttta aagattatat tgcggcacca aaggccaatg gttatcaatc
    14461 tattcacacg acggtttatg gacctaaagg gccaattgaa attcagattc gaactaaaga
    14521 aatgcatcaa gttgccgagt ttggtgttgc agcccattgg gcctataaaa aaggaatcac
    14581 cagtaaggtt aatcaagcag agcaatcggt tggtatggga tggattcaag aattagttga
    14641 gcttcaagat gaatcaaaag acgccaagga ttttgttgac tctgttaagg aagatatctt
    14701 tacagaacgc atctatgttt ttaccccaaa tggtgctgtt caggaattgc caagggaatc
    14761 tggaccaatt gattttgctt atgctattca cacgcaagtt ggagaaaaag ctactggcgc
    14821 taaagtaaat ggacgtatgg ttccattgac tgctaagtta aaaacaggag atgttgttga
    14881 gattatcaca aaccctaatt catttggacc aagtcgtgac tggattaaga ttgttaagac
    14941 caataaagct cgcaataaaa ttcgtcagtt cttcaaaaac caagataaag aaacttctat
    15001 taataaaggt agagaattac tggttgatta tttccaagaa caaggttatg tgcctaacaa
    15061 atatttagat aagaaacata ttgaagaaat ccttccacgt gtcagtgtta aaagtgagga
    15121 agctttgtat gcagccgtag gttttggtga tttgagtccg attagtattt ttaataaact
    15181 gacggaaaaa gaacgccgtg aggaggaacg tgctaaggct aaagcggaag cagacgaact
    15241 tattaacggt ggcgaaatca aaaccgataa gcgtgatgtt ctcaaagtta agagtgaaaa
    15301 tggtgttatc attcaaggag cttcaggttt attaatgcgt attgcaaaat gttgtaatcc
    15361 cgttcctgga gatctcattg aaggctatat aactaaaggt agaggcgtgg ctatccatcg
    15421 ttcagattgt caaaatttaa agagtcagga gaactacgaa caacgtttga ttgatgttga
    15481 gtgggatgat gatggatcta aaaaagagta catggctgaa atcgatattt atggtttgaa
    15541 tcgtagtggc ctccttaatg atgttcttca aaccttatca aatgcaacaa agctagtatc
    15601 gacagtaaat gcacaaccaa caaaagatat gaaatttgct aatattcatg ttagctttgg
    15661 aatttcaaat ttagcacaat tgacaactgt ggttgataaa atcaaaatta tcccagatgt
    15721 ttattctgtt aaacgtacga atggttagga gagcaaaatg aaaatcatta ttcaacgtgt
    15781 tagtcaagca agtgtttcta ttgaagatga tgtagtcgga tctattgaga agggtttagt
    15841 acttctagtt gggattgctc cagaagatac aacagaagat attgcttatg cagttcgtaa
    15901 aattactagc atgcgtattt tttctgatga tgaggggaaa atgaaccttt caattcaaga
    15961 cattaaagga agcgtcttat ctatctcaca atttacactc tttgcagata ccaaaaaagg
    16021 aaatcgtcca gcttttactg gggcagctga tcctgttaaa gcgaatcagt tttatgatat
    16081 ctttaatcaa gaattagcaa atcatgtttc ggtagaaaca ggtcaatttg gagctgatat
    16141 gcaagtcagt ctgattaatg atggaccagt aactatcgtt ttagatacta aaaataaata
    16201 aaaaatcaaa gagttgggca aaatgcttgg ctctttttat tgtggttcta attatactcc
    16261 ttaacccatt catgtttata aaaagattca ctgtagcttg gaaaggcatg aataaaggtt
    16321 gttgatatga aattaagtgc acttaacaat attaaaaata gaaaaatatt agatttatgg
    16381 tttttcgttg acactaaaat aagaatactc taaaatgtta actggttaag tactttttat
    16441 tttaaaggag gtttaatgaa aaggatacgg aaaagcctta tttttgttct cggaatagtt
    16501 accctaattt gcttatgtgc ttgtactaaa cagagccagc aaaaaaatgg cttgtcggtc
    16561 gtgactagct tttatccagt atattccatt acaaaagcag tttctggtga tttgaatgat
    16621 attaaaatga ttcgatcaca gtcaggcatt catggttttg aaccctcatc aagtgatgtt
    16681 gctgccattt atgatgctga tctatttctt tatcattcgc acacactaga agcttgggcg
    16741 agacgtttgg aacctagttt gcatcactct aaagtatctg taattgaagc ttcaaaaggt
    16801 atgactttgg ataaagttca tggcttagaa gatgtagagg cagaaaaagg agtagatgag
    16861 tcaaccttgt atgaccctca cacttggaat gaccctgtaa aagtatctga ggaagcacaa
    16921 ctcatcgcta cacaactagc taaaaaggat cctaaaaacg ctaaggttta tcaaaaaaat
    16981 gctgatcaat ttagtgacaa ggcaatggct attgcagaga agtataagcc aaaatttaaa
    17041 gctgcaaagt ctaaatactt tgtgacttca catacagcat tctcatactt agctaagcga
    17101 tacggattga ctcagttagg tattgcaggt gtctcaaccg agcaagaacc tagtgctaaa
    17161 aaattagccg aaattcagga gtttgtgaaa acatataagg ttaagactat ttttgttgaa
    17221 gaaggagtct cacctaaatt agctcaagca gtagcttcag ctactcgagt taaaattgca
    17281 agtttaagtc ctttagaagc agttcccaaa aacaataaag attacttaga aaatttggaa
    17341 actaatctta aggtacttgt caaatcgtta aatcaatagg tagttatata aagaaaggac
    17401 gcaggatgaa tcgtaaaaaa acagttatta ttagtgcttt atcagtagct ctatttggta
    17461 ctggtgtagg tgcttaccaa ttaggtagct ataatgccca aaaatcagac aatagtgttt
    17521 cttatgtgaa aacagataaa tctgatagca aagcacaagc aacagctgca aataaaacgc
    17581 ccgaccaaat cagtaaagaa gaaggcatat ctgcagaaca aatcgttgtt aaaattactg
    17641 atgatggtta tgtaacttct catggagacc attaccatta ttacaatggg aaagtgccat
    17701 atgatgccat cattagtgaa gaattgatta tgaaagaccc tagctatgtc tttaataaag
    17761 ctgatgtcat taatgaagtc aaagatggtt atattatcaa agttaatggt aagtattacc
    17821 tctatcttaa agaaggtagc aaacgaacaa atgttcgtac aaaggaacaa atccaaaagc
    17881 aacgcgaaga atggtctaag ggtggttcta agggagaatc aggaaagcat agtagtgcaa
    17941 aaactcaagc tttatcagcc agcgtacgtg aagcgaaagc tagcggtcgc tatactacag
    18001 atgatggtta tgtttttagt ccaacagatg ttattgatga catgggagat gcctttttag
    18061 taccacatgg cgatcatttc cattatatcc cgaaagctga tttatctcca tcagagttat
    18121 cagccgcaca ggcatattgg aaccgcaaga ctggccgatc aggtaatagc tcaaaaccat
    18181 caaattcaag ttcatacatt catgcaagtg caccaagcgg taatgtatca acaggaagac
    18241 atgctaatgc ccctatttct attccaagag tcactcacgc aaatcactgg tcaaaaccag
    18301 ctggtaatca tgctacggca ccaaaacacc atgcacctac aaccaaacca atcaaaaaag
    18361 attctgcttt agacaaaatg ttgaaacgtt tatatgctca accgttatct gctcgtcacg
    18421 tagaaagtga tggtttggtt tatgacccag cacaagttaa tgctttcaca gcaatcggtg
    18481 tttctattcc gcacggtaat catttccact ttattcacta taaggatatg tctccattag
    18541 agttagaagc aacaaggatg gtggcagagc atagaggaca tcatattgat gcattaggga
    18601 aaaaagattc tacagagaaa ccaaagcata tttctcatga acctaataag gaacctcaca
    18661 cagaggaaga acaccatgca gtaacaccga aagaccaacg taaaggcaaa ccaaatagcc
    18721 agattgtcta cagtgctcaa gaaattgaag aggcaaaaaa agctggtaaa tacacaacat
    18781 ctgatggtta catttttgat gctaaagata ttaaaaaaga tacaggtaca ggttatgtca
    18841 ttccacatat gacacatgag cattgggtac caaagaaaga tttatcagag tcggaattaa
    18901 aagcagctca agaatttctt tcaggaaaat ctgaagcaaa tcaagacaaa ccaaaaacag
    18961 gtaaaacagc tcaagaaatc tatgaggcaa ttgaaccaaa agcaattgtt aaacctgaag
    19021 atttattatt tggaattgca caagcgacag actataagaa tggtacattt gtaattcctc
    19081 ataaagatca ttaccattat gtggaattaa aatggtttga tgaagaaaaa gatcttttag
    19141 ctgattcaga taagacatat tctttagaag actatttagc tacggctaaa tattacatga
    19201 tgcacccaga aaaacgtcct aaagttgaag gatggggtaa agatgctgaa atttataagg
    19261 aaaaggactc taataaagca gataaaccaa gtcctgcacc aactgataat aaatcaacat
    19321 caaattctag tgacaaaaac ttaagtgctg cagaagtatt caaacaagca aaaccagaaa
    19381 aaattgtacc gcttgataaa attgctgctc acatggcata tgcagttgga tttgaagatg
    19441 atcaattgat tgttcctcat catgatcatt atcataatgt tcctatggca tggtttgaca
    19501 agggtggttt atggaaagca ccagaaggct atacattaca acaactcttc tcaacaatta
    19561 aatactacat ggaacatcct aatgaattac caaaagaaaa gggttgggga cacgacagtg
    19621 atcataacaa aggctcaaat aaagacaata aagccaaaaa ttatgctcca gatgaagaac
    19681 ctgaagattc agggaaagta actcacaact atggttttta tgatgttaat aaaggttcag
    19741 acgaagaaga accagaaaaa caagaagatg aatcagagct agatgaatat gaactaggaa
    19801 tggcacaaaa cgctaagaaa tatggtatgg atagacaatc ttttgaaaag caactcatcc
    19861 aattatcaaa taaatatagt gtaagttttg aaagctttaa ttatattaat ggtaatcaag
    19921 ttcaagttac taaaaaagat ggctctaaag tgcttgttga tattaaaaca ctagcagaag
    19981 tgaaataata aaagagttga ataaacttag ctgacaacta aaaacaaaca agtcaccgca
    20041 cttgtttgtt ttttagttat gatttctagc gtgcgaagat tttaatgata tatctaattc
    20101 ttcttttgaa caaccattgt tttacgctta aaagagagtt aatgtatttt tgtttgagga
    20161 agttttcttg ttttgacttt tataaaacga ctaaagttat aatcactaaa taaagagcca
    20221 ctactaaaag ctgtataaca ggtttctagt agtggctcac ttgttttatt acatgtaatt
    20281 gttattagtt aaaattttgg tgaagctact gtattgctcc ttaacaacat tatcaatatt
    20341 atgatctgtt aatacaaaat caatatcgtc taattgataa aaagtataaa aatcatattt
    20401 accaaatttt tggctatcaa ctaaaaggaa tttttcaaag gaattattaa gagcaatgcg
    20461 ctggatttca ccttctccct cgtcataagt agcgatagac ttttcaaaaa caccattact
    20521 actaacaaaa gcttttgaaa aattaagtga tttaatagag ttaatagtaa cagaaccgac
    20581 aaaagcacca gtaatttctc tgtactctcc tcctacaagg ataaggtcaa gtgttgaact
    20641 ttgattcaga atattgaaaa caggaaggct attggttact accctaattt taaaatcaag
    20701 taattgcgta gctaatttct ctaaggttgt tccaggccca ataaagattg tttcaccatc
    20761 attgatgaat tgttttgcaa attgggcaat ttctaatttt tcatttgttt gaatatcgta
    20821 tttttcagtg tttgattttt cataattttg tggaattggt gctgcgttta ctttttgtgc
    20881 accaccatgg atgcgtatta acagtcctgc tttatctaat tcatccaagt cacgcctaac
    20941 agtcatgtct gatacatcaa gttcttccat gatttcatta acagtgacaa taccatttat
    21001 attgactttt tctattattt tttgtaatct ttctcgcttt aacataataa tctctcctac
    21061 ctcttcattg tatatatcat accataaata gagctaaaat aataataatt ttgaaaaaaa
    21121 caaacaataa taaacaaaaa taaataaaaa acgtcttgtg atgttttatt ttgttttgta
    21181 taatattgat gtagataagt tgataaggct tgtttgcctt acaacaaaat gtaaccgctt
    21241 aactaaaaag aaaggtgttt gctgtgactc aagatatttt atttattgat gcccattctc
    21301 aggaagaatt atttgactta gtatcaaaag cattgataaa acaacactat gttagtcctg
    21361 actaccgaca ggcagtaaaa gaaagggaga gagagtttcc aacggggtta aaaattgatt
    21421 taaaagatgg gactcctatt caatatgttg ctattccaca cactgaaacg cagtattgtc
    21481 tagtagatag aatcttttat gtcaaaaata gtcagccaat tactttcaaa catatgatta
    21541 atccagaaga ggagtgtagg gttcaggatt ttttcttcat cattaatagt aggaatagta
    21601 accaaagtga tattttatct aatttgatta ccttctttat tacaaaagga aacctagatc
    21661 ggctacatga gcttggcgac aataaggaaa aaattaatca ttatttaata gaaaaaggag
    21721 tgttttaaca tgatcaaaat attagcagct tgtggtgcag gcataaattc aagtcatcaa
    21781 attaaagatg ctatcgagac acaattagga gatagaggtt acaatgtaca ttgtgatgct
    21841 gttatggtta aggatatcac tgaggaaatg gtcaacaaat atgatatctt tactcctatt
    21901 gctaaaactg atttaggttt taatgtacca attccagttg tagaagcagg acctattctt
    21961 tatcgcatac cagttatgag cgaaccggtc tttactgctt tagaacaagt tatcaaagaa
    22021 cacaacctat cataacatat ttaaaattgg aattagtctt aaaggagaag gaacatgcag
    22081 gtatttttaa atattgtcaa taaattcttt gatccagtta ttcatatggg ttcgggagtt
    22141 gtgatgctaa ttgtcatgac aggtttagcc atgatatttg gagtgaagtt ttctaaagca
    22201 cttgaaggtg gtattaagtt agctattgct cttacgggta ttggtgctat tattggtatt
    22261 ttaactggtg ctttttccga atcacttcaa gcttttgtta aaaatacagg aatcaatcta
    22321 agcattattg acgttggttg ggctccatta gcaactatta catggggatc accatatacg
    22381 ctttacttct tattaatcat gcttattgtc aatattgtta tgattgttat gaaaaaaaaa
    22441 cggatacctt agatgttgat atttttgata tttggcattt atctatcaca ggtcttttaa
    22501 ttatgtggta tgctaaacaa atgaaacgcc gtaatgcaga atatgcagct aatcaaaaat
    22561 aaatagaata aacaaaaacc taggaaatat cacattctcc taggtttttg atatattaaa
    22621 tattttaaaa ataatatata taattaattt tcgctcattt taataataaa aaactagtaa
    22681 aattataaaa atatattgaa aatagtgata agttggtata caatcctttt tggaaacatt
    22741 actgaactcc catttttcta gagttgatgt atgcaaccaa tattttaaga cttggagttt
    22801 ctatgacatt aacctttggg aaaccttcta taacgatacg ccgtctagga gtgataacta
    22861 gtacatatat gatgctaggt tttatcttat tatgtggact tttgcatcct agtacaattt
    22921 atagtgagga aataaaatct gatataaact cttttatatc aatagatgat gaaaaaacta
    22981 ctgctcccat atttgactct actttagata agcttactaa ttcaacagag gagaggcagt
    23041 tagaattaga ttctgataca tcggacggtt taatagtaga tgatcaaaaa gataaagata
    23101 tggtagtagt cttaggtgat tctgacagtc aagaaaaatt gtcacctcct ctaaaatcgg
    23161 aaatagcgac agaagaaaat cgtaaacagg caaatttagc agaactaaat gcaactcaac
    23221 ctaataatag aactacctat attatacccg aaagcagtca ttccattgca gaacaacaga
    23281 gattcctgat agaatcaaag ggttcttcgg ttgcattact taatagcgat gaatttagaa
    23341 agacagcggg agaggataga ggttttgaaa gggataagtt gaggtctttg gatatcattc
    23401 ctaagggaga tttatcgaca agtaatgtca taggtaatac ggacattgct agtcagatat
    23461 cgttgggctt taaaaagaat gcgatgcagg aacaccatct tactaaaaca ttctctcaaa
    23521 aggatggaaa gttatcgtct gttatagagg ggatgcttgc tattggcaaa gagaaagtag
    23581 agaaagaaat aaaatatagt ggtaatttat ggcaaaaatt aaaagctaag gcacactgcc
    23641 ttgtttgctg tgttgataat ttgaattttg aagatataaa atcttatttt caatattatt
    23701 gtcatctaaa ccatcagctc aaattaccta aaggtgctat actttctgct aaaacagaag
    23761 tatatagggg aggagatttt gggagaaaaa ataaagataa tgtgtttggt taccgtatcc
    23821 cctcattatt gaaaacccaa aaaggaactt tacttgcggg agctgatgaa agaattgagc
    23881 aagcttgtga ttggggaaac ataggaatgg ttattcgccg tagtgaggat gatggtgtca
    23941 cttggggaaa aagagaaact attgtcaatc tccgtaataa ccctagagtt ccgctagtta
    24001 ctagtggtga ctatagtggc tcacctatta atatggatat ggcattagtt caagatacta
    24061 gctccaagac gaaacgtatt ttttcaatat atgatatgtt tccagaagga agaggcgtta
    24121 ttagtattgc taacacacct gaaaaagaat atacccaaat cggaggacag tcttatctta
    24181 atttatataa taatggaaag aaatcgaagg tttttactat ccgtgacaaa ggtattgtat
    24241 ataattttaa agggaaaaag actgattatc atgttataac agaaactact aaaagtgacc
    24301 attcaaatct aggggatatt tataagggaa aacagctact tggaaatata tattttacaa
    24361 aacataaaac gtcaccattt cgtttagcaa aatcaagcta tgtgtggatg tcatatagcg
    24421 atgatgatgg taggacatgg tcatcaccta gagatataac agcaagtctt cgtcagaaag
    24481 gcatgaaatt tttgggaata ggacctggaa aaggtatagt tttaaaatgg gggccacacg
    24541 ctggtcgtat tattattcct gcctattcta cgaattggaa atctcatcta agaggttcac
    24601 aatcttcacg cctaatttat tcagacgacc atggaaaaac gtggcatact ggaaaagcag
    24661 tcaatgataa ccgtatactt tctaatggtg aaaaaattca ctccttaaca atggataata
    24721 aaaaagaaca aaatacagaa tccgtacccg ttcaattgaa aaatggggac attaagttat
    24781 ttatgaggaa tctaactggt aacctagaag tagccacaag taaagacggc ggggagactt
    24841 ggcaaaacca tgttaaacga tataaggaaa ttcatgatgc ttacgtccaa ctatcagcta
    24901 ttcgctttga gcatgacaaa aaagagtata ttttattagt gaatgctaat gggccaggga
    24961 agaagtgcca agatggatat gcacgtctag cgcaagttaa tcgaaatggt agttttaagt
    25021 ggttatatca ccatcacatt caagatggtt cgtttgctta caactctgtt caacaactta
    25081 ataatgatca atttggtgtc ctttatgaac atagagaaaa acatcaaaat agttttactt
    25141 taaattacaa agtttttaat tggagttttc ttagtcaaaa tacagagaag caaggcactt
    25201 tatgggagaa aatggcagca aattggcatg ttttgtttaa attttattta tgattatggc
    25261 ttgcggaata accgttccta caaataaaaa taatgagaag tatcaaactt cgtgatagct
    25321 agctagtagt gaattctagt tgccatagtt atcacggagt tttttttgtt tatttcaaac
    25381 aaaaacaaaa acaaaacatt gacaaaaaca aaaataagat atatgatgat attacaaaca
    25441 aaaaacaaac aatatcttcc aactacatac aattgtatta aaagtgaaat aaggagaaag
    25501 aaatgacaat tattattggg gcggatgcgc atggtgttga acttaaggaa gtgattagac
    25561 aacaccttac acgtttaggt aaggaaatta ttgatttaac ggatactagc aaagatttcg
    25621 ttgataatac cttagcaatc gttgctaaag tgaatcaaaa agaagataac ttaggaataa
    25681 tggttgatgc ttacggtgct ggtcctttta tggtagccac taaggttaag gggatgattg
    25741 cagcagaagt ttcagatgaa cgctcagctt atatgactag agctcataat aacgctagga
    25801 tgattacttt aggttcagag attgttggtc cggggttgct aaacatatag tagagggttt
    25861 tgttgatggg acttacgatg caggtcgtca tcaaattcgg gtcgatatgt tgaataaaat
    25921 gtgctaagcg taagagaggt aaagcagatg aaaattgcgg tcggttgtga tcatattgta
    25981 acttatgaca aaattgcagt tgtggattat ttaaaaacaa aagggtatga ggttattgat
    26041 tgtggaactt atgataacat ccgtactcac tatcctattt atggtaagaa agtaggagaa
    26101 gctgtcgcta gtggaaaagc cgacttaggt gtatgtattt gtggaactgg agttggtatt
    26161 aataatgctg ttaataaagt tccaggaatt cgctcagctc tggtacgtga tttgacatca
    26221 gctatctatg ctaaagaaga attaaatgct aacgttatcg gatttggtgg gaaaattact
    26281 ggtgggctgt taatgacaga tatcatcgag gcgtttataa gagctaaata taaaccaaca
    26341 aaagaaaaca aagtattaat tgaaaagata gccgaggttg agactcataa tgcccatcaa
    26401 gaagagaatg actttttcac agagttctta gataaatgga atcgtggtga atatcacgac
    26461 taaggagata tctatgattt taacagtaac tttgaaccct tctattgata tttcgtactg
    26521 cttagaaaac tttaacatgg atactgta
//
LOCUS       DATRME010000021        26397 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRME010000021 DATRME010000000
VERSION     DATRME010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26397
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(7..555)
                     /locus_tag="VBC23_000877"
     CDS             complement(7..555)
                     /locus_tag="VBC23_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6842840.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(622..1407)
                     /gene="codY"
                     /locus_tag="VBC23_000878"
     CDS             complement(622..1407)
                     /gene="codY"
                     /locus_tag="VBC23_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6842841.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(1533..2744)
                     /locus_tag="VBC23_000879"
     CDS             complement(1533..2744)
                     /locus_tag="VBC23_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6842842.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            3011..3463
                     /locus_tag="VBC23_000880"
     CDS             3011..3463
                     /locus_tag="VBC23_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6842843.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(3518..4900)
                     /locus_tag="VBC23_000881"
     CDS             complement(3518..4900)
                     /locus_tag="VBC23_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6842844.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIKTPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            4969..5931
                     /locus_tag="VBC23_000882"
     CDS             4969..5931
                     /locus_tag="VBC23_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6842845.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(5966..6844)
                     /gene="aroE"
                     /locus_tag="VBC23_000883"
     CDS             complement(5966..6844)
                     /gene="aroE"
                     /locus_tag="VBC23_000883"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6842846.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(6981..7856)
                     /locus_tag="VBC23_000884"
     CDS             complement(6981..7856)
                     /locus_tag="VBC23_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6842847.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKE"
     gene            complement(8147..10162)
                     /gene="recG"
                     /locus_tag="VBC23_000885"
     CDS             complement(8147..10162)
                     /gene="recG"
                     /locus_tag="VBC23_000885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6842848.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFE
                     INAHLELKENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(10242..11780)
                     /locus_tag="VBC23_000886"
     CDS             complement(10242..11780)
                     /locus_tag="VBC23_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6842849.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(11873..12973)
                     /locus_tag="VBC23_000887"
     CDS             complement(11873..12973)
                     /locus_tag="VBC23_000887"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6842850.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(12970..13329)
                     /locus_tag="VBC23_000888"
     CDS             complement(12970..13329)
                     /locus_tag="VBC23_000888"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6842851.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(13354..14361)
                     /locus_tag="VBC23_000889"
     CDS             complement(13354..14361)
                     /locus_tag="VBC23_000889"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6842852.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(14487..17015)
                     /gene="secA"
                     /locus_tag="VBC23_000890"
     CDS             complement(14487..17015)
                     /gene="secA"
                     /locus_tag="VBC23_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6842853.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(17124..18071)
                     /gene="manA"
                     /locus_tag="VBC23_000891"
     CDS             complement(17124..18071)
                     /gene="manA"
                     /locus_tag="VBC23_000891"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6842854.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(18189..19070)
                     /locus_tag="VBC23_000892"
     CDS             complement(18189..19070)
                     /locus_tag="VBC23_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6842855.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(19138..21057)
                     /locus_tag="VBC23_000893"
     CDS             complement(19138..21057)
                     /locus_tag="VBC23_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6842856.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            21242..22681
                     /locus_tag="VBC23_000894"
     CDS             21242..22681
                     /locus_tag="VBC23_000894"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6842857.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            22683..23645
                     /locus_tag="VBC23_000895"
     CDS             22683..23645
                     /locus_tag="VBC23_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6842858.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(23729..24163)
                     /gene="nusB"
                     /locus_tag="VBC23_000896"
     CDS             complement(23729..24163)
                     /gene="nusB"
                     /locus_tag="VBC23_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6842859.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(24156..24545)
                     /locus_tag="VBC23_000897"
     CDS             complement(24156..24545)
                     /locus_tag="VBC23_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6842860.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(24634..25194)
                     /gene="efp"
                     /locus_tag="VBC23_000898"
     CDS             complement(24634..25194)
                     /gene="efp"
                     /locus_tag="VBC23_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6842861.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(26122..>26397)
                     /locus_tag="VBC23_000899"
     CDS             complement(26122..>26397)
                     /locus_tag="VBC23_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6842862.1"
                     /translation="VYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFPEGLNLEQV
                     YYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT         8373 a         5238 c         4224 g         8562 t
ORIGIN      
        1 attcaactaa agaatagttg caccaagaac agttttaaaa tgatttaaag cccattgatg
       61 attactatca tttaagctgg ctacagcagc tgctgggacc tcaattttat aacctaagtt
      121 ataagcatca attgctgtgt gtaatacaca aatatctgtc agtactcctg tcaatatcaa
      181 agtatctact cgtctttcac gtaggcgaat atctaaatca gtaccagaaa aggcagaata
      241 atggcgctta tctatccaaa aaacacgact atcttcctta atagtttcat atagagttcc
      301 caaaggacca tataggtttc taccagaagt ccctttaata ttatgctcag gaaaaagatt
      361 ggtctctgga tgaaaaacat ctccaatatc atgattatca atcgcaaaaa aaatataatc
      421 ccctgaccgg tatgctttct ctgtaacgtc agctatagct gaagcgatag attgcgctgg
      481 tttacctgcc gttaatttcc catcatctgc cacaaaatca tatgtgtagt ctattgagat
      541 gagtgctttt gtcatttttc ttcttatctc ctcttgattt caaaaaagga actgaaagct
      601 actcccagtc ccatgctatg attaattata ttcttttaat ttgtcaaaaa taccttcgtt
      661 aataactttg aggtatgttc ccttcatacc gagtgagcgg ctctcaatga ttcccgcaga
      721 ctccaattta cgaagagcat ttacaataac ggaacgtgta attccaatac ggtctgcaat
      781 aacagatgcc gttaggcgtc cttcaagtcc atctaattct cctaagatag cagctacagc
      841 tttcatttct gaataagata gagtgttgat agccattgtc accgctgttt gtttacgaat
      901 attttcttct agattttctg tttgaaggtt aagtaattga ataccaacaa cggtgcttgc
      961 aatttcaact aaaatcaaat catcgtcgct aaattcttta tcattacgcc agataataaa
     1021 tgttcccaag cgcattccac caccataaat tggagcgatt gttgtaatac catcttggaa
     1081 gttttccttt gtctcaactg ggaaaattga caaatcatta tctactgata agttagcttc
     1141 tgtatcataa acacgactag cagatttaac atagtaatct gggaattgtt ttgtttcaaa
     1201 aaattcttcc acacggtcag tgtttgtttt atatttcatg gcatagccaa gtaagttacc
     1261 accaccatta ataatacatg cgttacagtc aataatatca gctaattgag ccgccattgt
     1321 attatatggt aattcggcat ctaagctatc tacggaacgc tgtaagatgg atgtaatttt
     1381 tctagttttt tctaataaat tcggcatttc cttcatcctt tgtatgtttt gcgttacaat
     1441 tatatcaaag cattttagct tttgcaaatc gatatcagta cattttaaga acttctattg
     1501 ttaatttaat gtttatctaa taaaaattca ttctagcgct tgtattggct taaaaagcga
     1561 gccatttttt cttgtagttc agtcaattca tcaacacgtg gtaagtaaac aattctaaaa
     1621 tggtcagcgg tattcatatt aaatcctcta ccgtgtgtta gtaatacttt ttcttgtttt
     1681 aagaaattaa gcacaaactc ttcatcatta tcaatacggt acatatctgt atcaatttta
     1741 gggaaaagat acagtccagc atttggtttt acagcagata aaccaggtat ttcattaatt
     1801 gctttatgga tataattacg ttgctcgtag atacgacctc ctggcaacaa catactatca
     1861 atagattgct gtccgcctaa cgatgtttga ataacctgtt gagcaagaac atttgaacac
     1921 agcctcatgt tagctaacat attcaaacct tcaatataac ctttaacatg ttgtcgcgga
     1981 ccagacaata ccatccaacc aacgcggaaa ccacagatgc gatgtgattt agataagcca
     2041 gataaagtaa ctgtaaaaat atcctctgca atagaagcaa ttgggatatg ctccattcca
     2101 tccataacta aacggtcata aacctcgtct gaaaaaataa tcaagtcatt ttgtctagcg
     2161 atatccacaa tctcttgcag aatttcacgc ggatagactg ctccagttgg attatttgga
     2221 ttaatgagga cgattgcttt tgttttggat gtgatttttg atttgatatc atcaatatca
     2281 ggataccaat tagcttcctc atcacaaata taatgcacag catttcctcc agcaaggcta
     2341 acacaagctg tccataacgg gtaatctggc attggaacta gaacctcgtc atcattatca
     2401 agcaaagctt gcattgacat agagattccc tcactaacac cattaacaat gtatatatca
     2461 tccatgtcaa catggatgtt ttgtaactgg taatactgca taacagcctt acgagctgaa
     2521 aaaatacctt tcgaatcaga gtaaccttct gattcacgcg cattcgtaat taaatcacga
     2581 atgacttcat ctggcgcttc gaagccaaag gcagctggat ttccagtatt taaccgcaat
     2641 atcttttcac catttgccct catgcggtcc gcctcttcta aaacagggcc tctaatatca
     2701 taagccacat gttccagctt cattgattta tcaaatatct tcataaaaat acctcgtatc
     2761 ttccattata aaacaaatat tctcaaaatt gaaacattta ctgcggttta tcaagcgatt
     2821 aagttaatat taagctattt cagaaaacta acataaaacg ctttcatttt tctagaaaaa
     2881 acgttataat atagctataa aatgaataac caacatatct aacttactca tcactcactc
     2941 cttctgataa gatggctagt tgagtgcaag aggttatctc ttttggttat atttagagga
     3001 ggacaggctt atgtcacaaa aatatgaacg catccttgtc gctattgatg gatcaacaga
     3061 atctgaactt gcttttgaaa aggcagttaa cgttgctctt cgcaatgatt ctgaattaat
     3121 cttgacacat gttatagata cacgtgctct acaaagcttt gcgacatttg atacttatat
     3181 ctatgaaaaa ttagaaaaag aagcaaaaga tgtccttgag gaatatgaaa aacaagctcg
     3241 tgaaaaagga gcagataaag ttagacaagt cattgaattt ggtaatccca aaacattact
     3301 tgcacatgat attccagaaa aagaaaaagt agacttgatt atggttggtg caacaggtct
     3361 taataccttt gaacgtttcc ttatcggatc ttcctcagaa tacatccttc gtcacgctaa
     3421 agtagattta ctcattgtac gtgaccctaa taaaacaatg taaatttaat acgctcactt
     3481 tctaaaatat cctgagttat attaactcag gatattttta ttttcgattt aaggatttct
     3541 gaatttggct atctaattca cgtcgaattg atggctcagg agcgaaatgt tcttcccagt
     3601 cgtctggttt taaaatttta tgagtaacag gatcaaaatg agctttgcca tctggaaaga
     3661 ttttacccat attggcctca tgtactgtat caaaaagagg ttttgggtcg actcccataa
     3721 gaacaaagga gccataagtc agataaagta aatctgttaa ggcatccact tctcccacta
     3781 atggtgtttc tggatggtct ttagaaataa ccttatctct tgctttatca atagctaagt
     3841 gtaaattacg gatagattgg tcaaatactt cttggttacc ttgactagca gcatacaaga
     3901 actcaacgat ttcttctact ttaaagtccg acctaaaacc agcttcctta ctgtcatata
     3961 agcgtggcgt tttaatagtt tcaccatcca ttaatagatg gaaagatttt actttattga
     4021 aattctcgtc acgactactg aatgtttttt caatctcaaa ttggataagt ccataatggg
     4081 ctaatgcctt agagatacca tcatcattat tgtcagcagt tgtataacgt gcattttctt
     4141 tcacttgctg actagcgttc cccatggcaa caccaatacc aacttgagat aacatctcaa
     4201 tatcattatc agaatctcca aatgctatca cttctgataa atcaaaacca tagcattgac
     4261 caacacgttc aattccctta acttttgact gcccttcaga aatgacatcc attgagtaag
     4321 gactgcttct tgttaattta acacgaggaa attgtttttg aattcgctct gattcgccct
     4381 ctgttgcaac taaaacgacc tgataaatag gttctcgtaa gataaccatg aggctatcaa
     4441 tattttgagg tttaatccga cgaataaaat gcttaaagct tcgttcaatc gcttttgccc
     4501 attttctcgg aacaaggcta gatacaatct gccccaaacg gctagttcct agaccaataa
     4561 tacctgaacc gagtaatcct gaggctgttc ctaatgaaat ttctcttctt cttgctccag
     4621 cataacgaat cagacgatag acagttgtct tagataattg attggtataa ataatttcac
     4681 ttctactata gatatattga ccattatagg ttacagcaaa atctaaacca aggttttcta
     4741 aaaaaggttg tacaaaacta ggacctcgcc ccgttgctaa accgactagt attccttggt
     4801 ctttcaaatt tcgaattgct ttaattgttg acttttgtac attcttacga tcattaagaa
     4861 gtgtaccatc aatatcaaaa aatactgcct taattgccac tgggtcaaca tcccctcttt
     4921 tttatgataa aataaattat atcacaactt gaaagaagta taaaatcaat gaaaaaaatc
     4981 cttgttctac acacaggtgg aacgatttcc atgaatgcta acgagaaggg acaggtcatg
     5041 tccagcgccg ataacccaat gaaatacgtt gatttatcat tagatgacct tgatttgact
     5101 gttgtagatt ttctaaatct tcccagtcct caaatcaccc ctcatcatat gcttgatatc
     5161 tatcactatc tgaagcaaca tgccagtaat tttgatggag ttgtcattac gcatgggact
     5221 gacactttag aagaaacagc atatttttta gacacaatga tactccctaa aatcccaatc
     5281 atcattaccg gtgctatgag aagcacaaat gaacttggta gcgatggcgt ttataattac
     5341 ctatcagccc tccgtgtcgc taatagtact aaagctgccg acaagggggt gctagttgtc
     5401 atgaatgatg agatacatgc tgctaaatat gtcacaaaaa cacatacaac aaacgtttcg
     5461 acattccaaa cccctacaca tggccccctt gggattatta tgaaacaaga cctgcttttc
     5521 tttaaagcaa ctgaagagag agttcgtttt gatttagata aaattacagg aactgttcct
     5581 atcgtgaaag catatgctgg catgggtgat agtggtatca ttagcttttt aaattctcaa
     5641 aatattagcg gattagttat tgaagcactc ggtgcaggga atatgccccc aaaagctgca
     5701 caagaaatcg aagaattaat tgagcaaggt gtccctgttg ttctagtgtc tcgctgtttt
     5761 aatggtattg ctgagccagt ttacggatat gaaggtggcg gagcaaaatt acaagaatct
     5821 ggtgtcatgt ttgttaaaga actaaacgct cccaaagcca gactcaaatt actcattgca
     5881 ctcaatgcag gtctcacagg gcaaaatcta aaagactata ttgaaggttg ataaaaaact
     5941 ctagaaatat ttctagagtt ttttattaat tttcagtttg taatactctc tcaacactgc
     6001 taataggcat ttcttgattc gttattaatt gaaatgcttc tgctccttga taaatcagca
     6061 tccctagacc attataagca tgtttcactc cattttgtct aacaaatcgt aacaatgctg
     6121 tttctttagg cttgtaaacc aagtcgacaa ctactaaatt cggtgttaaa atttctggat
     6181 cgttaattaa actataatta tctaatggcc tcattcctaa actagtagca tcaatataaa
     6241 aatgactagt tctaatagcg tctttaaatg ctgtcttatt ttctagataa tcaacgacta
     6301 cctttatttg aaactgtttt ttaattttat ctgataagtc aatgacctta tcgtaatttg
     6361 agctgttacg attaaataat ctaatttccg caactccctc catagctgct tgaactgcaa
     6421 ctgctttacc tgaaccacca ataccagcta ttgtaattat tttattttta gcactgaaac
     6481 cttgagctgc taaagcttta aaacaaccaa tgccatctgt catatggcct actaaacgtc
     6541 cggttccacc ttgattaacg atagtattta cagcacccac taatttagct tgaggagata
     6601 aatcatctag caaagggata acactctgtt taaatggcat tgaaacatta acaccacgaa
     6661 tactcaatgc cctgacacct cgaacagctt ctgttaattt accctcttct acttcaaatg
     6721 tcagataggc ataattcatg tttttttctt gaaaagaggt attccacatt aacggggata
     6781 gagagtggcg tgcaggattg gcaattagac cagttagtaa agtttcccca tttaatgaat
     6841 tcattgtata accttctttc ttaacaattt atttcagttc attttaccaa aaactgactc
     6901 aattaaaaag agagtatgta atttatactc tctcccttag tttaaggcaa tatattacct
     6961 gcactacggt aaatatcata ctattcttta cgggttaaag cgatatcatt agccttatca
     7021 atcgccttta aacgagatgg attcattgaa ccaacaatcg cctgcatttt ggcaggatga
     7081 cgtaaaatcc atgcaatcac aatagcttcg ctgctaacat tgtagttttt tgccagttta
     7141 gctatggttt catttaattc cttataatcc ggatgattaa caaataaacc acgagataaa
     7201 tcaatttgaa atggagacca agcttgaatg gtaactcttt taagacgaca ataatcaaca
     7261 atgccatcat catgttcaat tgaagcttta tttaacatgt taacatttaa ccctgcatca
     7321 aacattggtg tatgtgctgg cgataactga agttgattca ctgctaaggg ctcgtcaagg
     7381 taggattgta acaactccat ttgaaaccgg ttttgattgc taacaccaaa gtgttttact
     7441 ttaccttctg ccctcagttt ggtaaatgct tctgctactt cttcaggctc aactaataca
     7501 tctggacgat gaagaatgag aaaatctaaa tattctgtcc ctaatctttc taaaatacca
     7561 tctactgatg ataaaatata ctctttagaa aaatcaaagt aaccttctcg tatcccacat
     7621 ttagattgaa taatcatttt gtcacgattg acatttaaat gtttgattgc atctcgaaag
     7681 cgtaattcag atagccctcc cccataaata tcagcatgat caaaaaaatt aatacccaaa
     7741 tctaatgctg ttccaactac ttcttcagct tgttttcctt tcaagtcact cattctcata
     7801 caacctaaag caattcgagt tgcttgaatg cctgtttgtc caatgttttg taacatatgc
     7861 accccattct aatctgtttg tattaacaga aagattaatc aattcattat aacaaaattt
     7921 ctatgaaaag gcttactatt acaaatgcaa aaagctgagt aaatttaact caactttttt
     7981 tctgatactc attattgact aaataagtcg gtaagaaaaa tctattctga aaagtactat
     8041 tataaattac agtaatcatt attgtcataa catacctcca catctgtgat aggaatatca
     8101 tatgacagca agcttaaact gagcttagca cagccttaat agaaacttaa tcaaaatctg
     8161 aatgttggcg taaattatct agaataagtg cccattctgt attttctttc caattattat
     8221 ctttgacaat atcactagca acacgtctgg cttcttctaa aataggataa tcctcaacga
     8281 tatcagctac ttgaaattct ggaattccag actgacgagt accaaagatt tcaccagaac
     8341 cacgcatttt taaatccgac tcagctaaaa cgaaaccatc tgtcgtttct gtcatgattg
     8401 tcattcgttt tttcccagaa tcagttttgg gattagccac taaaacagcg tatgattgtt
     8461 tatatccacg accaacacgc ccacgaagtt gatgtagctg gcttaatcca aaacgatcgg
     8521 catccataat aatcatgatc gttgcatttg ggacatttac ccctacttca ataactgttg
     8581 tagatactaa aatatgactt tttttatcct tgaaatcttg cattatagca tctttttcat
     8641 catttttcat acgtccatgt acaagagcaa ccttagcaat tccttcaaaa taagtagata
     8701 attcagcatg caatgctact gcattcttta aatctaaagc ttctgactct tcaatcaacg
     8761 gtgaaatgac atacacttga gcatcttttt gcaattcacc ttttacccat tccaacacag
     8821 tacctagctg ctcgtgtttc acccagcgtg taataatagg tttacgaccg gcaggtaatt
     8881 catcaataat agaaacatcc atttctccaa aagctgtgat tgctagagtt cggggaattg
     8941 gggtggctgt catcattaaa acatcaggat tttctccctt ttcacggaaa attctacgct
     9001 gtttaacacc aaaacgatgt tgctcgtctg taattacaag ccccagttta tggtactgta
     9061 ccgagtcctg gataagagca tgagttccta caatcatatc aaccgagcca tttgcaatag
     9121 ctgctaaaac cgtacgctta acagctgctt tcatacccga agttaatata gcgattgata
     9181 aatctggaaa taactcttgc agactaatgt agtgttgttc agctaggatt tccgttggaa
     9241 ccatcaaagc cgattgaaaa cctgctgtat aagctgcata cattgataga ccagcaatga
     9301 ctgtctttcc tgatcctaca tctccttgca ataaacgatt catatgagct cccgatgaca
     9361 tatcagataa tatttcatct aaagagcgct tttgagcatt cgttagaata aaaggtaaag
     9421 atgaaatctt tgtctccata gcatgtttac tataaagaat agacaaacca tttgtttcag
     9481 atttattttc ggctttcaaa acttgaaggt tcatttgaaa gtaaaataat tcttcaaatt
     9541 taatccgacg gagcgcttgc ttatactctg tgatatcttt tgggaaatgc atagctaaac
     9601 aagcctgact acgacccatc aatcggtatt ttcccaataa agtagctggt aaattttcct
     9661 tcaattccaa atgcgcattg atttcaaaag ctgacttaat agctttaatc aaagtagact
     9721 gtgaaattcc ctgagatacg cgataaacag gttgcatgtc atcttcaact tgagctaaaa
     9781 ccttcatccc agtaatagct gatttagttg catcccattt accaaagaca gctacctctt
     9841 gaccaagttc tattttatca gctaaatagg gttgattaaa aaaactaaca tttaagacag
     9901 cttccccttg acgcaatttg aaacttaaac ggttacgttt aaaaccataa tattgtacat
     9961 tagctggagt aacgactaag cctgtaatga ctgctttttc accatccact aaatcaaaaa
    10021 cagattttga tttaaaatct tcatagcgaa acggataata aagaagtaaa tcttctactg
    10081 tataaatatc taatttctga aatttttctg ctgattttgg tccaaatccc ttaagattag
    10141 aaattggtga ttgtaatagc ataagattct ccttctaata tcttttatta tactaaaaga
    10201 tcaccaaaaa ggcgcctcaa gagcgccttt attatgtcta attatttatg gccaataaca
    10261 taagttaatt gtgcagcttg tgctgaacta aaagtacggt aagagacaat accacgtccc
    10321 attgcattag attctgaaat gagaactgaa ccatcttttt taacttcttc aacaatagct
    10381 acgtgaccgt aagtgccatc agcaccagct tgtcctggtg aaaatgatac agcatagcct
    10441 acttttggtg aatgtgttgt ttcaaagcca gccttatgtt gccaatctcc accattaccc
    10501 ataaatggat caaatgtata acctaactct ttagcgcggt taaagacata ccatgtacat
    10561 tcaccccatg gataagttga tgcaatatag ctagctgtgt taaccgctgt tgataagcta
    10621 aaaccacttg gtaaatctgc taaacttgct gtcgataaag ctttcaaggc agcaggaatt
    10681 tctggtgtcc ctccatgttg atcaatccag cggtctaaat cttccataat ctgggcacga
    10741 cgtttaccag ttccagagtt atctgtataa taaacaccac cctcagtaac tgtattaagt
    10801 tgtccagaag ataatttctg tgctttcaaa tcttggattt caaagctaga gttgttgttt
    10861 ttaataattg tattttttgt caacaacata attgcttctt catcattata agcactagca
    10921 tggctagagt catgatcaaa ggcaccataa ccaaacatat tagcacctgg catcttagcc
    10981 actccttgag ttcccaatga actttcaagg acagcaatag ctacaatagc acggacatct
    11041 aaaccactat ctttttccca ttgtaataac ttagcaccat tgatacgatt agaatcatat
    11101 tgaatcccta aagattttaa gtacccatct aattgttcag ctttgatacc gtaacgatgt
    11161 gacaataaat agtgagagta ggcatcctga ccagaccagt gttctacgta gaatggcgtt
    11221 gctgtcactg gtgaaatcgg tgttgataat aagttatcat caaaggataa agcaggaaca
    11281 gaagcattct gatttgtctg aacactttct atctgagacg gagcgggagt tattttaggc
    11341 tctactaatg gtaaggtagg ctcaggtttt aattcagtct ttgttgattg acctggctta
    11401 ctgtctgctt gactactgtt tgtagtatta gtgctaggag tagatgctgt tgaagcagaa
    11461 tcagatgttt tagcatcttc gttggaagaa gattgatgtg atgaacttga tgatgattca
    11521 ttgcttgatg atgtctctgt cgtaccaatc tctgtttttt ctgttgagat acttccttcg
    11581 gctgaaggtg aagcagtttc tgaatctgta ggttgtgttt tttcctgttt ctctgcttca
    11641 gaagaacttt ctgttgatgc tgttgccatt gtcaggcttg tttctggatt aatagtttct
    11701 gctaaggtca ctaaaggtga tgatagcgat aagactaaag tggataacaa caaagttgcc
    11761 gttacttttt gttttgacat atactaatga ttcccttcta taaaaattgt cactgattag
    11821 tctaacatat ttttaaattt tttaaaacag ttaactactg tgtcatttgt ttttaataat
    11881 atctcggaat acgatcgctt aaaagacaaa gtacttcata attaatcgtc tgtctttttt
    11941 gagcgacgtc cgtagttgta atgttacaac taccttgttg accaatcaag gttactttag
    12001 ttccgatagt atatttttga ggtaaacgga tagtcatttg atccatcgag acacgcccaa
    12061 taatttcaca aagttctcca tttactataa ctgaaaatcc ttgcatatct cgtgtccatc
    12121 catcagcgta gccaatcggt actgttccaa caaattcgtc tccagtaact tgataggttg
    12181 cgccatagcc aacttgagaa ccatcgtgta gttgtttaac atgtactagc tcagactcta
    12241 aacttaaagc agggtttata gggtaaggta agtctaaatc agttccactt ggatttaagc
    12301 cgtacataac aacacccaat ctaacggcat taaaaattgt ctcactatgc caaaggcttg
    12361 tagcagaatt actagcatga acaaggtccg gacaattatc taatcctgaa ataaagtctt
    12421 taaaaaaagt aagttgttga ttgaatttac agttatcggc ttcatcagct gttgcaaaat
    12481 gggtgaaaat ccccttgaca gaggcaccac tttcccccaa ttcagaaatt agcttatttc
    12541 cttcatctaa ctggcgaaca cctatacgtc ccattccact atcaactttg atatgtactt
    12601 ctagaccaga taaatctact gctgatgtct gacataaacg taaccactcc aagctagcaa
    12661 ctgttagagt aatattttca tttttggcta gtataacctg ctcaggcata acaacaccta
    12721 ataccaatat catttttaca attcctgctt gacgtagctc aatagcctca tctaaattgg
    12781 atacgcaaaa accatctaca atagactcaa tgtattttga aacttcaaca gcaccatgcc
    12841 cataagcatt agccttaact acagcaaatg tttttttatc acttggaatg tgctcttgta
    12901 cagatttaac gttatttgca atcgcctcta aatctatcaa ggctctggtt ggtctatgat
    12961 aacttgaaat catttttctt cctccaaaat aacactagct tgtgcataat taccactatg
    13021 agatattgaa acaaaagatt ttccattaaa cggttctttt gtaatcaaag gcgctccttt
    13081 atcatccgat aaaatttcga tatcatgaaa attaactttc ccaattcctg ttccaagtgc
    13141 tttgctataa gcctcttttg ctgcccatcg ccctgttaaa aaagacatct gacgcttggg
    13201 attggaaatt cctttaaaaa gaagcaattc ttgttcggtc aaaacgcgtt ctgcaaaacg
    13261 ttgattacgc tcatatgctt tagtaatcgc ctctatctct tgtaaatcaa ttccgtgtcc
    13321 aacaatcatg tgtaactcct ataagttcga tacttacaaa ctattgtaaa tatccctaat
    13381 tagcatttct gttttatccc aacctagaca tggatctgtg atagacttac caaaaacatc
    13441 tggtttatct tgtcttccat cttctaaata agattctatc aaaaaaccac gaacatagtt
    13501 tctaatttta gtatgccact gtcggtctgc aagtactgat ttcacaatgc gaatttgttc
    13561 taaaaattgc ttacctgaat tgtcatgatt tgtatcaaca acaataaagg gattttgcaa
    13621 atcagtctct tcataatgag taatagtttc taaaaggttt tcataatgat aattagggat
    13681 acttcgatag tcggcattat gatatcctct caaaataaca tgtgacagca aattaccatc
    13741 tgttctaact tgcttatttt gatagaataa ttcctgttga ttttgtgctg catagaccgc
    13801 attaaacatg actcttaaat taccagacgt tggatttttc atacctactg gtgcatctat
    13861 acctgaagat ataaaacggt gtccctgatc ttccactgaa cgcgcaccaa tagcatagta
    13921 agaaactaaa tcatccatca ctgataacat ctctggataa agtaattcat cagctgtcag
    13981 gagtccagtc tctcgaatga tgtcatagtg catcttacgt gcttgaaaaa aaactcctag
    14041 tttgtcagaa tggtgaacta agcctttata accatcacca ttcgttcttg gcttagcggt
    14101 ataaactctc ataaccaaaa acattttatc tttaaaggca gcttccaatt tagctaaacg
    14161 ctttgcataa gttagcaccg cctttggatt atccgcagaa caaggaccta ctattactag
    14221 aaaccgctga tcctcacctt ttatgatatt ttttaactct tcttctttgg ctaatttttt
    14281 ttggctaact tctagatcta agctagtttt ctgcttcaat atttcaatat taattttatc
    14341 acttaactga tgaaatccca tgttttttcc taatacaacc taagtctatt aataagctct
    14401 gctatacaat atagtcgtca tactattaga caagtaatga taaaacagtc ttctcagact
    14461 gttttatttt tattttaaaa tcattgttat tgtaaacatc tcagaccatg acaattttta
    14521 aacttcatgc cagagccaca aggacattta tcattacgtt taatcccttg gtattctggt
    14581 gattcattat tcataggtac atgttgtgcc gagatatttt gctctgctgt tgtcgtagca
    14641 tgttgagaag cacgttcacg ctcttgttca tgaatttgag ctttcatcaa tgtacgtgtc
    14701 acatcaaact caatcgaacc aatcatatct tggaacatac ggaaaccttc tgattgatac
    14761 tcaacgattg ggttattttg agcataacca cgaagtccaa cggagttacg taattgatct
    14821 aaggcatcaa tatgctctgt ccattgatta tccactacca tcaagattaa gactttttgg
    14881 aattcgataa tagcttctgg atcttttaat ttagcaattt gttgctcata aacagctaag
    14941 gcacgttcat aaagaagttc tttaatttca gcctctttta gaccacgaag ttctgaaact
    15001 gtgatagctt cttcatctaa taacgccgat cttgcaaaat taacaatagc ttctgctgca
    15061 gtatttttat cgcttctgct atgtgcatca actgctcttt taatcgtacg tttaatcatc
    15121 cctttgagct ctgatcctaa atcacgctca gcagtaatca cttctctacg gttagcataa
    15181 ataatctcac gttgttcacg cataacatca tcgtattgca agacttgttt acgtgtgtca
    15241 tagttattac cttcaacgcg gcgttgagct gattcaactt gtcgtgtcaa catttttgat
    15301 ttaataactg tatcatcttc agctaaattc attcgctcaa gaaccacttt aatacgatct
    15361 gtaccaaagc gacgcatcaa atcatcttct aacgaaagat agaattgtga ttcacctgga
    15421 tctccttgac gacccgaacg accacgaagc tggttatcaa tacgacggct ttcatgacgc
    15481 tctgtaccaa taacacataa accaccaagt tcacgaaccc cttcaccaag cttgatatca
    15541 gtaccacgac cagccatgtt ggttgcgatt gtaacagcac cacgttgccc agcattcata
    15601 atgatttgag cttctttaaa gtgattttta gcattcaaaa cttcatgtgg tactccagca
    15661 gcaactaatt ttctagaaat taagtctgac gtttcaacag ctactgtacc aaccagtaca
    15721 ggttgtcctt gttcataacg ttctttgaca tctgctacca ccgctctaaa tttagagtca
    15781 agagttggat aaagtagatc tgaatgatct atacgttgaa caggacgatt tgttgggata
    15841 ggaataacgc gcatattata aatttcacga aactcttctt cctcagtctt accagtacca
    15901 gtcattcctg ctaatttatg gtacatacgg aacatatttt ggtaagtaat tgaagcgctc
    15961 gtttttgatt cttcttggat tggcactgac tcttttgctt caatagcttg gtgtaagcca
    16021 tctgagaaac gacgtccttc catcgtacga ccggtaaatt ggtccacaat taaaatctct
    16081 tgttcttcac taaccacata atcaatattt aacaacataa tatagttagc tcgaagagcg
    16141 ttatcaatgt agtgagttaa ggcaacattt tcgagatcat acaagttatt tagatggaaa
    16201 tagttttcag ccttgtcaat acctgtatct gataaaccaa ttgtttttgt tggaacatca
    16261 atgatataat catcgctatt aagcgttttt acaaacatgt ccgcacgtgt atacagttgg
    16321 ttcatctcag agcttacagg acctgaaaca atcaatggcg tcctagcttc atcgattaat
    16381 acagagtcaa cctcatcaac caaagcatag ttgagtgggc gttgcaccat atcttcttgg
    16441 cgaacaacca tattgtctcg aaggtagtca aatccgactt ctgcgttagt tgagtatgtg
    16501 atatcacaat tgtaagcttc gcgtttttca aatggtgact tagctgctaa gttaatccct
    16561 acagataatc caagccaact ataaacctca cccatttcgg tcgcatcacg tgttgataag
    16621 tactcattaa cagtaataac atgaacacct aatcctgaga tagcatttag ataaacaggc
    16681 attgtcgctg ttaaagtttt tccctcacca gtacgcattt ctggaatatc accatggtga
    16741 agaacgatac cacccataat ttggacgtgg tatgggtata aacctaatac acgtttagaa
    16801 gcttcacgaa ctaccgcaaa cgcttccggt aacaattgat ctaaagtttc accattttga
    16861 taacgttctt taaattctgg agtttttgct tgtaaagctt catctgataa cgtagccata
    16921 tgatctgcgt aagagtcaac tttcttagct attttatcta atttttttaa ttcacccttg
    16981 tcattttcaa taacagtgcg tagaatattt gccattctaa ttcctttcga tactcttatc
    17041 accacatttt accataaata atgttgtctt tcaataaatg gtcgttttaa tcgagattaa
    17101 aacaagtggt atccccactc tcattatttt ttaacatgac ttgctataat ttctaactgt
    17161 ccatcaatat cccatttgac aacatcattt ggcaagataa agtggtcacc ttttttaagt
    17221 gtataaactt tgttatctac tgtaatgtga cccgctccgt ctaagacact aaccaaaagg
    17281 tagggtgcaa attgtttaaa gttggtaaca ccagaaattt cccatttata aactgtaaag
    17341 aaatcatttg aaactaataa agttgaagat agatgtttta acttcatatt ggcaggaaca
    17401 gtattggcag gtttaccaat cgtgagaaca tctattgatt gttcaatgcg taaatcacgt
    17461 aattttcctg aagcatctgg acgatcaaaa tcataaacac gataagtagt atcactagat
    17521 tgctgtgttt ctaaaattaa tatcccttta cctatagcat gcattgtccc acttggaacg
    17581 tagtaaaaat cccctgattt aacaggaatt cgggttaaaa gatgttccca atctccagat
    17641 tcaatcattt ggcgaagttc ttctttagtt ttagcattat gaccatagat aatttcagat
    17701 ccttcatcag cagaaataat ataccaacat tctgttttac caagttcacc ttcatgttta
    17761 agagcataag catcatccgg atgaacctgt acactaagcc agtcattagc atctaagatt
    17821 ttagttagta aaggaaagac agtatcatca gggttgccaa ataagctttt ttcaccttga
    17881 tacagcttat ctaaaaaaca acctttaaag cgaccattct taactcttga gacaccgtta
    17941 ggatgagctg aaatagccca atattctcct gttgtttcac ttggaatatc atagccaaat
    18001 tcatccctta actttgttcc accccagatt ttatcgtgca tgcttgcttc taaaaataat
    18061 ggttccgaca taagaatccc ctatcttttg taaaattcaa gcctattata gcattttatc
    18121 catcatatcg cttacttttt ttaatctttt atttgctaac taaactaagt agtggcttaa
    18181 gggttttatt aaccttttga tattttcttt gctaaagcaa aatttcctaa cgttgcagaa
    18241 ccattttctt ctatagctgg tgtcacaata taatcagaca aatcgggaac tggtaaatac
    18301 ccatttaata aagttgcaaa tgtctggcga acacgtctta acatgtgctc ttgtgccatg
    18361 acgcctccac caaatacaat gacttgtgga cgatataata cagttgcttg tattgcagcc
    18421 tgagcaatat aataggcttg aacatcccaa accattgaat tttcttcaat caattcacca
    18481 cgaataccag tcctagcttc tagagttgga ccagatgcca aaccttctaa acagctatta
    18541 tgaaacggac agataccttt aaaatcattt gcttggtcct gcggatgcat cgccatataa
    18601 gtatgacctg cttcagtatg accagttcct cctataaatt cacccttttg tatagctcct
    18661 gcaccaattc ccgtaccaat ggtgtaataa acaagtgaat caatattgtt acgagctata
    18721 acttcaccat aggcagagct attaacatct gttgtgaaat aaaatggaac attgaaatct
    18781 ttagaaatta agccgagtaa atcaacatta gcccaatgta actttggggt cgttgtgata
    18841 taaccgtatg tctttgattt tttatcaata tcaatgggac caaaagaacc aatagcaact
    18901 gcctctaact tcttttcaaa tcttttaaag aaatcaacag tctttttaat cgtttcttga
    18961 ggcgtcgttg ttggaaattg cattttttct acaactttta attcctcatc accaactgca
    19021 caaacaaact ttgtcccacc tgcttcaata ctaccatata actttgtcat cttttttcct
    19081 atctataatc tctatctcaa agacaaaggt tgggataatt atcccaacct ttaatcctca
    19141 cttaacatca ataatcatgt cacctgtagt aacaacaccc tcttttacca atggtataac
    19201 atcagcaaaa tcagctgtat tagtcacaat aatcatagct gtattatcca gacctgcttc
    19261 tgcaatcttg gctgaatcaa aggtgccaag aatatcacct tttttcactt tttggtctgc
    19321 cttgacttta gaagtaaacc ctgtgccatt catggtgaca gtatcaatac ctatatgaat
    19381 caaaatttct gcacctttat ctgatttgat accgtaagca tgacctgttt caaaagcaat
    19441 ttggacaaaa ccatcaacag gtgagtaaat tgtattacct cttggtttaa tagcaaggcc
    19501 atctcccata atacctgatg aaaaaactgg gtcacttact gtatcaagag caataacctg
    19561 tccatcaaga gggctaacaa tcgtttctgc ccttaattgg ctagtttgag taattgttgg
    19621 atcgtcatct gtttcttcaa ccaaaggact aacttcagct actgcttttt catctttatc
    19681 ttcatatcca aacatataag ttaatacaaa agccaaagac gtagttccaa ttaccataat
    19741 aaggtactta acaatttgac catttaaata aagtaatgtt cctgggataa tggtaatacc
    19801 aaaaccagtt cctgctaatt tcaatattga agctaaccag ccacctgctg ctcctgcaat
    19861 taatcccata ataaatggct taccaaaacg taagtttact ccaaatatag ccggttctgt
    19921 aataccaagt ccagctgaaa gcgctgccgg aaaagcaagg gcttttaatt ttttattttt
    19981 tgttttaaca ccaactgcca acgtggcacc tgcttgtgct gtcatagcag ctgtaataat
    20041 agcattaaat ggatctttcc catcagcagc aattaactga gcttcaagta aattaaagat
    20101 atgatgaaca cctgtaacaa cgattatctg atgaacacca cctaaaataa gacctgatag
    20161 gccaagaggt aaattaagga caaattttgt ccctgctaaa acatagtttt caactgaatg
    20221 gaaaatagga ccaatgacaa aaagtgctaa aattgacata actgtaaaag ttaggaacgg
    20281 cactaatagt aaatcaagaa catctgggat tttcttatgc aaccattttt caagtttagc
    20341 accaagtaaa cctacaaaga atgccggcaa tactgaattt tgataaccaa ctactggaat
    20401 aaagccgaaa aattttatgg gatgggcatc acctgaggcc actgcccaag cgtttggaag
    20461 ggcagagttg accatcatca atcctaatac gagaccgata acaggatttc caccaaaaac
    20521 tctaaaagct gaccaagaaa tcaaggcagg aaaaaaagca aaggccgtgt cagtcaaaac
    20581 gacagtatag gtataaaaat tagaacttga aaatgctttt gaagtcgtcc caaataaagc
    20641 taaaactgta tcattattaa tcgcaccgcg aatccccata aataaaccag tagcaacaat
    20701 ggctggcaac aatggaacaa aaacatcacc gaaagtacga atagctcgtt ggaaagcatt
    20761 cccttgttta gccgcttctg ccttttgatc agaggttgac aaagttggca aaccttgagc
    20821 cactacttca tcataaatct tattaacagt accagttcca aaaataattt gatattgacc
    20881 tgaattgaaa aatgctccct gaactttttc gatattttca acagtatttt tatcaatgac
    20941 tgtctcatct tttaccataa tgcgaagacg agtggcacag tgtgctacgc tacgcacatt
    21001 actagcacca ccaatcgcat taatgacttg tttagcaatt tctgtatttg aagccatcta
    21061 taaatctcct ttgacatttt gaaagcgttt cattttattt actctaatat tctagcatgt
    21121 ctcataaata tatgtcaagc gtttgacata aaataatatt ttttgatgtt aagagttgat
    21181 ttcaaaagag aaaacgttta ctatatagtt attagatatt aagacagtca aggagtatca
    21241 aatgaattta cccactgaaa tccgctatcg tccttatgat gaatggactg aagaagataa
    21301 ggaaaatatt ttaaagaatg tctcaaaatc cccttggaga gcaacttatc acttagaagc
    21361 taaaacaggg cttttaaatg acccaaatgg cttctcttac tttaatggaa aattccatct
    21421 tttttatcaa aattggccct ttggagctgc acatggttta aaacaatggg tacatacaga
    21481 atcagatgat ttagttcatt tcaaagaaac aggaatcaag cttaaacctg accatgtcaa
    21541 tgatagccat ggtgcatact ccggttctgc attagccatt gacgataagc tttttctttt
    21601 ttatactggt aatgtccgtg acatgaaatg gaaccgtgac ccccgccaaa ttggagcatg
    21661 gatgacaagt gatggcaaga ttacaaaatt tgataaagtc cttatttcac aaccaaatga
    21721 tgtcactgag cattttcgtg atcctcagat tttcaattat gataatcaat tctatgctgt
    21781 tatcggggcg caaaatagta aaaaatgcgg attcattaaa ttatataaag cacttaataa
    21841 tgacatccat cattgggagt ttgtgggaga tttagatttt ggtggtactg gatctgagta
    21901 tatgattgaa tgtccaaata ttatttttgt caaaggaaaa cctgtattac tttatagtcc
    21961 tcaaggacta gataaaaacg aattggacta ccaaaacatt tatccaaata cctacaaaat
    22021 cggacaatat ttcgatgcta actcatctaa gatagttgaa ccatccccta tctataattt
    22081 agattatggt tttgaagcct atgctactca aggatttaat acttctgatg gtcgtgcctt
    22141 tatcgtgagt tggataggct tgccagatat tgattatcct agcgatcaat ttgattatca
    22201 aggagctatg agtttagtta aagagttaag cataaaaaat gggaaccttt atcaatatcc
    22261 cgttcctgct atgaaaaatc ttcgtcaaca tcaagcagaa tttaaaacac aacttcaaac
    22321 taacaacacc tatgaattag aattactagt tcctagaaac gatctttctt catttgttct
    22381 ctttgctaat ccgaaaggac aaggactatc cattactatt gatactgcaa aggggaaagt
    22441 tatcgttgac cgctcacaag ccggacagca gtacgccaca gaatttggta ctagtcgcca
    22501 gtgtgacatt ccaaaagatg ctactagtat caatattttt atcgataagt ccatctttga
    22561 aatctttata aataaaggag aaaaagtatt tacgggacga gtctttccag atgcagaaca
    22621 atcaggaatt caactaaaag aaggacacgt tcatggtaaa tattttgaat taaaatatta
    22681 atatggttgc aaaattaaca gatgttgctg cacttgccgg tgttagccca acgactgtat
    22741 cacgcgttat taacaaaaaa ggctatttat ctcaaaaaac agtcaccaaa gtcaatgaag
    22801 ccatgagaac ccttggttat aaacctaata atttagcacg tagcttacag ggaaagtctg
    22861 ctaaattaat tggactcatt ttccccaaca tccgtaacat tttttacgct gaattaattg
    22921 aacaccttga aattgagttg tttaagcatg gttataaaac tatcttatgt aatagtgaaa
    22981 aggatcctat caaagaaaaa gaatacctcg aaatgctagg agcaaatcaa gtagacggta
    23041 tcatttcaag tagtcacaac ctaggaattg atgattacga gaaagttgag gctcccatag
    23101 tagcattcga tagaaattta gctccacaca tcccgatcat ctcatctgat aatttctttg
    23161 gaggaaaaat ggctgctcaa acccttaaaa aacatggctg tcaaaagatg attatgatta
    23221 cagggaatga taattcagat tctcctactg gacttaggcg gctaggtttt tcctatgaat
    23281 caaaggaatc caaagttatc acagtcacaa atgggctatc taacatgaga cgtgagatgg
    23341 aattaaaatc aattatctcg actcacaaac cagatggtat ttttacatca gacgacttaa
    23401 ctgctttatt agtaatcaaa cttatcagcc aactagggtt gtccatccca gaagatatca
    23461 aagtgattgg atatgatggt acaagtttca ttcaagatta cgtcccacat ctaaccacta
    23521 ttaaacaacc tattcgagaa attgcgcaat tgatggttga aatactccta gctaagattg
    23581 aggggcaaaa gacaaataag gattatattt taccagtcag tctcatccct ggtagtagtg
    23641 tttaggaaat catatttata aaataatttt tagtaatagt tatttttcaa agcaaattaa
    23701 aaatgtggaa gaatcttctt ccacattttt aattttcatt tgttataaat tggcttaata
    23761 aaccattgac aaattttgct gacgtctcat ctgaatattt tttagcaatc tcgataatct
    23821 catttagagc tacacgatca ggcgtttcat caaaatattt aatttcatag aggcctaagc
    23881 gtaagagaga tttatcaacc aatgttagac gttctaagct ccatcctgat tttaagtgac
    23941 tagaaatcaa agtatctaat tcatccttgt gatctacaac accattaaca agattaagta
    24001 aaaagattgg aacctctaaa actttgtctt cagatactgt tttatcataa ccataagcaa
    24061 agtgagccgc atcaataaac tctccaccag tttctaagct aaatagtgtt tgaaaagcac
    24121 gttcacgtaa atcacgtctt gaatctttaa atacactagt catctaagaa atcctcatca
    24181 aacaatgact tcaagtctgg ttttggtgtt ttctctggaa caataccttc aacatggata
    24241 ttgaccgaag aaatttttac ttctgccatg tcatatacag ctgttttgac agcttgttga
    24301 atagcaattg aaactgctgg tacatttaca ccatactgca agtaaacata aatatctgca
    24361 gctactgtat catcttcttc atttttaagg tagacacctc tacctagaga tttcttacta
    24421 aggctatccg ttaccgcttt gttacgtaac gaatgaacac catcaacttt agttgcagca
    24481 atacctgtaa ttacctcaag tacacgtggt gaaatgacaa tttctccaag attttcagtt
    24541 gtcatagctt atcctttctt ttagtgttta ctaaggtttt tccttagtag agtaaattca
    24601 tcatgaatct acttaacact tttttgagag tcattaggcg cgtgaaacgt aagttccttc
    24661 tgctgtattg attactaact tttgtccagc ttcaataaag tctggaacgt taacaacaag
    24721 tcctgtttcc atagttgcag gttttcctga accagttaca gtagcacctt tgattgatgg
    24781 ttgagtttca gcaactgtca attcaacagt tgttggaatt tgaacaccaa taacttctgt
    24841 tccatagaat tgaattttta cttctgagtt ttcaagaatg tagagtaatt cattttcaat
    24901 gttaacagtt ggaatttcat attgatcgta agtttcatta ttcatgaagt aagctgtatc
    24961 atccatctta taaaggtatt gagcaggtac agtttcaata atagcttgct caaatttttc
    25021 ttcaggacgg taacttgtat caaaagttga accagtacga acatcacgca atttcatacg
    25081 catgattgtg ttacctttac ctggtttgtg gtggctagct tcaagaacac ggattaattt
    25141 cccatcagcc gtttcaaaag tcatgcctgc tttaagctta cttgcttcaa tcatttatta
    25201 atacctcttt gtaaaaatta tttatcaact cattttatca caaataacct gctttctcaa
    25261 atagtttatt gatttggtat gtgtctgatt tcttgataaa taaataaaat caccaagagg
    25321 gcgtaaataa gactgaagca cttgacactt tcctcttact tcagttttga taacagtttt
    25381 aatattatac cgatatcttg actaagattg aaatactgtt taggtgtttt tagtgacttt
    25441 ttagtaaatt ttaataaagg tagtaaacta tagttgtctt attgaaacag ataaaataaa
    25501 tcacctacgg agctattcgt atgaaaatgt agttattctc gatataagtt ttagatacga
    25561 acttaatttg ctctattgaa gctggtaaag gaaatcgggg tggtgttagt ggacgtgcgc
    25621 taagcattgg tactgagctt ggtgatacca atcttgctta ctacatcagg tagctctctg
    25681 ttttagtgcc atatgcaatt ggaataagag ctgtcattgg tattgaagga gccctagtcc
    25741 actgatcatt gaattactaa tttaggaggt ggaatgatga attttaatat gtctcattat
    25801 agaaagttac aactatgtcg ttttctaatc gtacagatac ctgcgctggc tttattagct
    25861 attcttttat acgggatttt cctaaagtac agtctatttt caaaagtatt ttttcttatc
    25921 ttttaggggt tgaaatagtt atcaattttg ggaagtacat accagtttac accaaccaca
    25981 atttgtcgag taaagaattg taatttagta aagccttttt cgttttcttc tgataagtgt
    26041 atcatgacat ctatcaatag catattcaca ctagtagcat attgatttgt ttttgagtag
    26101 aatttacttt ttagattatt tctattctac actagtcttt ccctttggaa gattctttcc
    26161 ctctgtattt ttccattttt caatgagacg agaaactctt tcttcctcct catcatggta
    26221 accttcaaaa acaacctcat caatatcttc ttgattaaaa tagtaaactt gttcaagatt
    26281 taaaccctca ggaaaaattc cacctaggta atcaaaataa acttcttgtc cttcttgctc
    26341 aacaattgca cctctattta caataactaa tttttgattg ccatcaataa gatagac
//
LOCUS       DATRME010000022        24850 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRME010000022 DATRME010000000
VERSION     DATRME010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24850
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..139
                     /locus_tag="VBC23_000900"
     CDS             <1..139
                     /locus_tag="VBC23_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842863.1"
                     /translation="EKDYLKNTDLAYVVCDKIVDADSIGFVYHGEYETWHFKLLNTEA
                     N"
     gene            529..963
                     /locus_tag="VBC23_000901"
     CDS             529..963
                     /locus_tag="VBC23_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO6842864.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
     gene            1129..1207
                     /locus_tag="VBC23_000902"
     tRNA            1129..1207
                     /locus_tag="VBC23_000902"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1587..1925
                     /locus_tag="VBC23_000903"
     CDS             1587..1925
                     /locus_tag="VBC23_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6842865.1"
                     /translation="MRPQKLTIAGGRRTTVDYDDRSAEYRDYNRNRWKYDKQVKQFYN
                     SKLWRETSKQVLLQNDYVCAMCGGEATMTDHIVSVKKDWNRRLDWNNLQASCKACNDS
                     KAIRERCKNN"
     gene            2096..2563
                     /locus_tag="VBC23_000904"
     CDS             2096..2563
                     /locus_tag="VBC23_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEO6842866.1"
                     /translation="MGRKLKVVETTKKHLTKEEKIARETAQNKASDGLDKLQITPPRH
                     LNEVARAEYRRIINDLQTLPVRNLDRGLLELYCSWYAIYKETTKKLDEIGYFTNDPDK
                     GLIPSPLILTLEKATANIRSSASQLGLTVDSRMKMFIPKEEEKQKSIFDKFGG"
     gene            2578..4332
                     /locus_tag="VBC23_000905"
     CDS             2578..4332
                     /locus_tag="VBC23_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6842867.1"
                     /translation="MTIEYDYSAISDIYKDDTFYYAKKIVDEEIKASKKVFKACLRHL
                     NDLKRIEDEDFKFIYLPQKASDPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLASLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIT
                     DEGYFGYVAEQDNEEEIKDETNWIKSNPILEVDTLHDKLMDYLRTRRKVSLETGEVNK
                     VLIKNFNMWRQSSEESYIDKQSWELAKIDKPDTYKRRVWLGVDVGRVSDLFAISPVVM
                     MDDYWYVDSFSFVATKYGLTAKEKRDGVSYSNLERQGYCEITTLESGVIDDERVLEKI
                     EELIYINEWEVHGICFDPYQFGTLLTMIEKRHPEWPLIEVSQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAANNAYIKTDNNGMRIDKNKNSNKIDPLDAVLDGYAVCYLEPF
                     DGSGYWTSEKILGGETLF"
     gene            4329..4496
                     /locus_tag="VBC23_000906"
     CDS             4329..4496
                     /locus_tag="VBC23_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842868.1"
                     /translation="MIGFILKNIHTLILLVGLGLLIYGLFLFGDKVGFIASGIILVIL
                     AIYVDSVGGRK"
     gene            4493..4723
                     /locus_tag="VBC23_000907"
     CDS             4493..4723
                     /locus_tag="VBC23_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842869.1"
                     /translation="MNKRIKKKRKLEHYIASLIAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKNNVTLPIERR"
     gene            4730..5950
                     /locus_tag="VBC23_000908"
     CDS             4730..5950
                     /locus_tag="VBC23_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6842870.1"
                     /translation="MSFFQPLGTSKVSYDDYIASVLAGDVSQQYLGVSALKNSDVLTA
                     TSIIAGDIARFPLVKKDINGDIIHDEDINYLLNVKSTNNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTGQALQFQFYRPSETTVEETENHEIIYTFTDTLTAKEIKCFAHDVIHW
                     KFFSHDTILGRSPLLSLGDEIDLQTGGINTLIKFFKDGFSGGILTMKGAQLSGEARQR
                     ARQDFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSEIGLKTLNDGDRRLYHVEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLVELGKQKSKDPNMDRYQSSLNYVFLDKKEEYQDQVGV
                     KGKRGEVNAKENKP"
     gene            5928..6593
                     /locus_tag="VBC23_000909"
     CDS             5928..6593
                     /locus_tag="VBC23_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEO6842871.1"
                     /translation="MPKKINLKGPLIANNSQEVYDYYGMEAVSAKSIIDKLPEDNSDI
                     ILEVNSNGGLVTVGSEIYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNWVSGNSDDLDKASSALKSSDKAIVNAYVAKTGKSEDEIMQLMRNETFMSA
                     QDAVESGFADEVMSFEAIASIDNQMLPQAVIDDYYASRSKRKQEINNMLLEIEKEEIL
                     QGL"
     gene            6617..7801
                     /locus_tag="VBC23_000910"
     CDS             6617..7801
                     /locus_tag="VBC23_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6842872.1"
                     /translation="MFDEKIEEIKADITELNKVIAAKTAEVKAALESDDLETARSVKA
                     EVEATKAKLVESENDLKLYESSVEVGGAENIGGKEVTQEEKTYRESVNDFIRSKGKIV
                     NDSLRFEGKDEVLMPINETTPVEPKKDGVKKTDVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHQAKKASGKWPVLQRATEKMVSVEELEKNPKLGKPQFTNVAWEIKTYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNDAIATVLKEFEAKTVKNLDEIKKLLNVELD
                     PAYNVSFIVSQSFYQTMDTLKDKNGRYLLQDSITSVSGKVFLGKPVFVLADEVLGKDT
                     AFVGDFKRGVLFADRKDLGLRWADNEIYGQYLQAVLRFGVSKVDDKAGYYVTFKPEQL
                     PS"
     gene            7815..7976
                     /locus_tag="VBC23_000911"
     CDS             7815..7976
                     /locus_tag="VBC23_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842873.1"
                     /translation="MAQPEVSSATVVGKPTKDSTIEDIKKYLTSQNIDFSGKTLKADL
                     LKLAGVEEV"
     gene            7979..8281
                     /locus_tag="VBC23_000912"
     CDS             7979..8281
                     /locus_tag="VBC23_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6842874.1"
                     /translation="MTVSKELLDSVKLYCKIDFDFEDDIIEEMIASAQEQICFAIEEG
                     STPEMFKNYAKFSLAVKKQVKEEYDHRGLSADTNRYPLANGVLNIIHQLRLRGDGA"
     gene            8278..8625
                     /locus_tag="VBC23_000913"
     CDS             8278..8625
                     /locus_tag="VBC23_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEO6842875.1"
                     /translation="MITRKMNVRIAIFSQTGGQNEDGEVVSAIRKDLYTCWAEVSKTQ
                     LRDFNYQSRYQNASNLPTNKDTKVFLIRYNPKLSIDNTMFIEHNKHIYKIDKIEFDES
                     GKEMIILSGVSIS"
     gene            8622..8999
                     /locus_tag="VBC23_000914"
     CDS             8622..8999
                     /locus_tag="VBC23_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6842876.1"
                     /translation="MTKGLDEILANITKLEVKSPKIAKEAVTQVAEEFEKALTKNTPV
                     DYSVHDTKLKYDTAISGFKGANVGITSKKIGYGRKTGWRAHYPNNGTIYQKGQDFKEK
                     TINEMTPRAKEIYAKKIKEGLGL"
     gene            8996..9421
                     /locus_tag="VBC23_000915"
     CDS             8996..9421
                     /locus_tag="VBC23_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842877.1"
                     /translation="MIAETTAYKLLKNDRTLNELLDKLRGGPFKNGFDQGIFTYDIPD
                     NPVDLRKVELAPFMRINTTYDGPSLYADDDMISNEQRITINFWCKTASQSDQIASCID
                     RILKNGGFERYTANEKPRYKDSDIDLLMNVRKYRYFDFY"
     gene            9437..10033
                     /locus_tag="VBC23_000916"
     CDS             9437..10033
                     /locus_tag="VBC23_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6842878.1"
                     /translation="MGKVKFGLRDFQYAVLGDNDKVNEKDKGIKALPGMKSAKLDITN
                     ELVTVMADDGPYVVLSGGITETKLEIEVLDLTSEARQDFFGIKADKGIERYNKSLTPN
                     NVACMFRTSDENGKAIWVGLLKGKFNIPGMDTKTKEGAPNPEPDKVTGNFVARGTDGD
                     VVVIGREGVNGFELKKFKELVFDGVTGLEKSSEDNSLM"
     gene            10086..10406
                     /locus_tag="VBC23_000917"
     CDS             10086..10406
                     /locus_tag="VBC23_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842879.1"
                     /translation="MYEITLKKGGVDKTFSKDFVNVEDNMLAVEHQVRQSAIFSDDKR
                     RLDDKEHRKLNESYLQMFVEMYGNQFNVDDLKQSNVSVLDKLTELFVDALGGEKEEDE
                     KKEQ"
     gene            10436..10603
                     /locus_tag="VBC23_000918"
     CDS             10436..10603
                     /locus_tag="VBC23_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842880.1"
                     /translation="MWIQSLLKNGYTILDIKKMRLSDIELMVKALETDFAEKEEVVET
                     TLDKAFPFLFG"
     gene            10616..14557
                     /locus_tag="VBC23_000919"
     CDS             10616..14557
                     /locus_tag="VBC23_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6842881.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTYMKTLDNSLKAVENSFN
                     GHGSRVKGLKAIYTETGGALKSYQELLKKQSQKYNELKENIGDVNKATASQKQALINA
                     KSAMMETAAKVSELQSRLKALGAETSVFTRFGNAAEQAGKKMKSFGDSVSGVGAAFTK
                     GVTAPIVAGAGYALKAAVDYEDAFAGVKKTVDEVKDSNGKVVYSYDMLSKGIRQMAKE
                     IPASTTEISHVAEAAGQLGIKTKDVLGFTRVMIDMGKSTNLSSEEAATALARFANITQ
                     LDPSKYSNLGSSIVELGNNFATTEKEIVEMGLRLAGTGKVVGLTDPQILGLATAMSSV
                     GIEAEAGGSAFSRVMQKINTQVLSGGEDLWKFAKIAGKSADEFAASWKKNPQEAIIDF
                     VKGLKRFKEEGKDVTAHLQDMDINSVREIDTLQRLAGAGDLLGDAFKSANKGFSENKA
                     LMNEANKRYETTASKLQMLKNQVNDVAIEFGGPLIDALRDGLEAGKPIIKGVADLAKA
                     FSSLDKEQQQQIIKWGLIAAATGPALSIFGKGVGVIGGTIQGLGKLSKTLGTLSGSLG
                     AAKTGAAALGVGAEGATTALTGMSGAAALLGNPVTWGVVLGGAAALAVGYFAQKAYEA
                     HQRTQEWGTKVSQVQANELQSFKDKVDQTNQSMEGFRGGAEQVNSVKTAFQGLVAEIE
                     KLENKDLSEKIKLAEQFGFSQGTMDQIKKSSRQTVENVKQMSDEVINIYQNASNEHRR
                     LTEEEKAVVLANQNELINVQLSKLNYSAKEKKAIVKAMNGDLNSLNSQQLTKALEVTE
                     KWIKAENKSYNKLKSGLKKVYDSIKGNDKEAVKTREEIHKKQQQLEADHYLKMEAYGK
                     RYAAIQKKLLKGTAKYLDPQLQQAMVNDVKKQMKELGLSYEELMTKTTKAASKAQEVN
                     TMWARTTKKSTEDQKLANSQWNGLVWNPKTGKLKTNAKEEVAKALEAEGGWDRLKFIA
                     KNANLETNARITMAEVLVETGKWDTLTPEEKELVVDGHQGIQAIVESKQHLEIWNSLP
                     EEVKRILGDNKDFLNKKGVATRTLENWNALKPDEKKLLAKNLTKKGKDEAQKTINSLV
                     GKEVKVTAANKTLSGVNSAQRTLNSVQDKHVTIWASIKKTASDLWSKLTGYAVGTDYH
                     PGGLAMVNDQKGSLYKELVTLPNGQSFIPDGRDVILPLPRGSKVMKASATRDYMYDLG
                     IPKYANGIGFDNTKIANITQRMSELPKNTVTSTTDDKLYWMIEEMIAVLKSTKDNSVI
                     EQALDIAEQAIERPVELYLQDGQWVAKVADRITNYQTQRNSRNNRMKGMLN"
     gene            14554..16077
                     /locus_tag="VBC23_000920"
     CDS             14554..16077
                     /locus_tag="VBC23_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6842882.1"
                     /translation="MKFIYDGVDMSKFFRISRVERSIGNERTLSLNETFQLGTAIRGI
                     KTGAKIIKVHIEPLEINGVLTEQLKHELAGVLNVDKPKKMTFGDEPDKYYLGLAQGEI
                     STEKVARWYQRAVITFYIPDGVAHSITYKKFSDYTQEGNKLIFNLQNDGNTNAYPIIK
                     IKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNESLFDYSKAIAQALEGAPNVAKL
                     NYMPPTYDTELKRMRIDNILGSGKGGEYVVIGNRGTTPGYTEHIGTRTFIINPDSNGE
                     YTLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLYGIETYKRKNGFETEYNF
                     FALDDDGVGWRFYKQFKFQADRNYHNPFSMNRSRAVEIFREEDKFRIYFNGAHHHVTV
                     PSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNNIVNKYQPGDEVVI
                     NFENDTVKTKDLDSIQDMVLGSQPISIPPGKTEFVMQLSKFSQYAPNVEILMEERWL"
     gene            16068..19889
                     /locus_tag="VBC23_000921"
     CDS             16068..19889
                     /locus_tag="VBC23_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6842883.1"
                     /translation="MVIITLVIHDSKLHPVLLLDNEKQGTLNYFDDTWTRQLTTGSSV
                     FEFSVYKKTLEGDNPLNHKYQVLNDQAFVSFVHKDKVQLFNIMQIEETETTVRCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARILSIANNFDAEIEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQSIINYAVQYNILPSGIICQLYLESLWGDSAVGKRDNNWAGISGGAQT
                     RPSGVKVTTGMARPPSEGGTYMHYASVDDFLKDYTYLLAKQGLYNVVGKKNIADYTKG
                     LFRVGGAKDDYAAAGYQSYTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSIGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSK
                     TRVTVLEQNFVGRMYVVENSYDINSFASGLQTVCYPREIAQGMAVNGATTQQVSGGTQ
                     ISYEEVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEIETDSQDVLMSTGLKDLKAHAYPAITYEVDGYVDLEIGDVV
                     RIQDDGYQPPLILTARVIEQVLSKDNPNSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYEIKLATSNGVAFKNGTGESVLTPSLQKNGKDYEAVYFYKNGDSLIDIGPSLIVK
                     ASDFNHVLNVTVEAYLNEELVASTQISFTDTEDGADGNTPYWHVAYANSADGTVDFSV
                     SDSANKRYIGQYTDYDAIGSNDPKKYKWTDMVGTVVVGTNNLIDGTKSFSGEDWFTSA
                     TLEDENISNYPFTFKKWISGQKVSHAKDIMVEQGVTYTFSAYVKREVAGNLYFYLYDI
                     ADGFITSDTPRETIIKNVDSSLRRFEITFTPTKTGRIRPRFAMVSSEQGSFSSGGFML
                     VRGNKTGDWQESEADKASNLDAKADGAFTVEQLNALAERARIAEAELQSKATLDTVND
                     WVKALQDEIKAREEGQKLSEQKLIDSSNRMIAVQQTIGEMQVRTDFVNKFMSQSEDGL
                     VIGQKDGTSSVRVDNDRISFYSSGKEVAYIAQSVLVIDSGIFTTKLQIGRYRIEQYEL
                     NPDINVVRYVG"
     gene            19902..21905
                     /locus_tag="VBC23_000922"
     CDS             19902..21905
                     /locus_tag="VBC23_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842884.1"
                     /translation="MTTYYSNSDRSYRLTYIVDEVSTSIADNSSQVRFRLYLTSRTNS
                     YAQYNFSGYAWVGGRYDFSAPSALGFNSNYLLIDKTIKVPHDADGSKTVVVAAKLSGP
                     GGYAPGTLNIPDQKFTLTKIPRTSTVAVSSGYFGDTLNVNINKTNDSFTYDVRYNVNG
                     ITGTVASDISGSTTFKTSLDWASTIPNTTSTPATIYVDTKSNGSVIGTSTGIFYLTVP
                     DNIKPKISSLTLSDTNQKASTIVGANNFVQIISNPVVTFNGASGAYGSTIQNFNAEII
                     GKNQSTQSNGGSLGILNFSGKATVKATVTDSRGRVSDPVTTEINVIPYSPPAFSFTVT
                     RAGAKNDQLVVTRNAKISPLIVDGVQKNKMTLTFKTAPLNTTSFTIDTSNASGTYTTV
                     AELINSTATLSGSYGANKSFDVYGLLSDVFSASGGGTPVKQTVSTESFPLSWHKNSVG
                     IGTLPKIDDTGSLNVAGNIYSDGKQIQQKQLALNNGGAFRHDTTDLNSLQDTGFYCVF
                     KGDNRPSGAGPGYLTVVRHETANYAYQHFYDRTNKTIFTRVLENGAWSGWSEYAKKDS
                     LPQSAPAVEDTGWQYIGNGFNYRKIGSMVTIKYDFATNGINQFTVGSMPTNLIPNEMM
                     FAVTAWTVQLNVLNVQVSADGRILWFNPSKWAVNVKGQINWII"
     gene            21917..22309
                     /locus_tag="VBC23_000923"
     CDS             21917..22309
                     /locus_tag="VBC23_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6842885.1"
                     /translation="MLEFLNRYPVLLEDKSVKETKAILAFTSSTIKANFEVTLPAEEN
                     DKKFAETLKTCEKLIFEQLYKDKAEAEQFEKINDAIAKSKAQSDKAENMIKLMSATVN
                     DLIKTMADGGKLNDTTLNNASENSSTHI"
     gene            22469..22771
                     /locus_tag="VBC23_000924"
     CDS             22469..22771
                     /locus_tag="VBC23_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842886.1"
                     /translation="MNIDILQIGAASGAILSVVGLWAFVVNPFKTAMQKNEDTMSALK
                     DTIKELAYELKDSQRDREKIHKILDIHEQRLGKTEDDIIVNKEQIKTLFNRRNKYD"
     gene            22764..22991
                     /locus_tag="VBC23_000925"
     CDS             22764..22991
                     /locus_tag="VBC23_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6842887.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQVGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALNYHEPKK"
     gene            23117..24451
                     /locus_tag="VBC23_000926"
     CDS             23117..24451
                     /locus_tag="VBC23_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6842888.1"
                     /translation="MATYQEYKNRSNGNAYDIDGSFSAQCWDGYADYCRFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFDEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFFGQNQGGTNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTDNTGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPGKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWVAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGIAG
                     GLDKNVVLLADDNSQVTVPKADKPQEAVSFNQRLDVNTKLDNSNTPYYEATLSTDYYV
                     ESKPNASSSDKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLANATQV"
     gene            24615..24821
                     /locus_tag="VBC23_000927"
     CDS             24615..24821
                     /locus_tag="VBC23_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6842889.1"
                     /translation="MSLVEEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAK
                     GKELADDAKDAVEEAVDAVKEKLK"
BASE COUNT         8641 a         4158 c         5400 g         6651 t
ORIGIN      
        1 tgaaaaagat tatttaaaaa atactgattt agcatatgtt gtatgcgata aaatcgttga
       61 tgcagactct ataggcttcg tttatcatgg tgaatatgaa acttggcatt ttaaactatt
      121 aaacacagaa gcaaattaaa gtcccacgca agcgcctaag agcctgcaat ggctctgtgg
      181 gtctacgagc tggaatactc gttaaactta ccctagaagc tttctgtaag tattcagcta
      241 cgtagcgtgg aataatcgtt acgtagttat agagcgaaat ttttagaaag ggaaatatcc
      301 tccgacattt tttcataaaa atctaaagtc tgttatcgct cacagatgat tatacaagcg
      361 caatgctgca aataaagtgc tgacgcaaaa ctaaaaattt aatactcgac aatttaacaa
      421 caaaaataag tcagcagagg aaggaaagga gaacaataaa aaagccggct cacgcaagcc
      481 agctccgata tatttgtcta ctaatattat atcatacgag gagctttcat gacgtttttt
      541 ccagaaatta atatacaaaa aactaaatca aacgctaagc gaaaactaag agagtatcca
      601 cgctggcgta ggatcgctaa tgatgtagat actcaaaaag tgacagccac ttattccttt
      661 gaacctagac aatcacatgg aacccctagt aagccggttg aacgcttagc actcaatcgt
      721 gtgtcagcgg aacaagaatt agaagcaatt gagcaatcgg ttagtatgat actagagcca
      781 gaaaagcgca ggattttgta tgacaaatac ttatctcctt acaagaatgc agataaggtt
      841 atttatacag aattatgtat gtcagagagc ttttattatg acacgctaga tgctgcatta
      901 ttagcttttg cagagcttta tagggagggc tctttgattg tagagcaagg agtttttgac
      961 tagtttttat acagtaatac aatagtttat acataaaaat atgtgttaat atagtattat
     1021 caaaatagca agaagagata atcatttacc aacaggctat ttatttagtc gtcaacttta
     1081 actactatcg aacttgctat tttgtagcta aaaggcgaga tagggtgttg agacgtagct
     1141 cagttggggg agcgatatga ctataaaggg tctggaacgt acgcaggttc gaatcctgcc
     1201 gtctcaatca gcgtcagagg agacgcgacc cagtatcccg aaaggcagtc acgcactgat
     1261 atactggttt ttacttggtt gatagtcata ttcctaccaa tgacgattgg taaaaaagat
     1321 acatagatcg gctgtatagt ctacgtgtac gtattcacaa cagattgact catttgatta
     1381 accacaagta aaacaagggt cgcaaccttg cttgtggtta gtagggacaa tggttgtaag
     1441 tcggttcgat tccgactgtt cctgttcgat aaatagaagt gtcccagaat ggggtaggca
     1501 ataggcttag cattcatttg ctatttatct atggttaacc aattagtcat cacattgtgg
     1561 tggcttttta ttatggaggt tagggtatga ggccacagaa gttaaccatc gctggtggta
     1621 gacgtacaac ggttgattat gatgataggt cagcagagta tcgcgactat aatcgcaatc
     1681 gctggaagta cgataagcaa gtcaaacagt tttataactc taagttgtgg agagagacaa
     1741 gtaagcaagt cttgttgcaa aacgattatg tctgtgctat gtgcggtgga gaagcgacta
     1801 tgactgacca catagtctca gttaagaaag attggaacag aagattagat tggaataact
     1861 tgcaagcaag ctgtaaagcg tgtaacgata gtaaagcgat aagagaaaga tgtaaaaata
     1921 attaattgga aaacgtgcgt aaaattaaca acagcacgtt aatgttcgga aaataccccg
     1981 cttgtttttg aacagggcta tatcgttcgt gtttttgaga acgcgccctt ttccgtgcaa
     2041 aatattccct ttttgaaatt tttgaactgt caattttcgt gtaaaggagg taactttggg
     2101 aagaaaatta aaggtggttg aaacaacaaa aaaacacctc acaaaagaag aaaaaatagc
     2161 aagagaaacc gcgcagaata aggcttctga cggacttgat aaattacaaa taacgccccc
     2221 tcggcatctc aatgaggtgg ctagagcgga atatcgtaga atcataaatg acctccagac
     2281 cctacccgta agaaacctcg atagaggcct tctagaactc tattgttctt ggtatgcaat
     2341 ctataaagag acaaccaaga aattagatga aattggttat tttacaaacg acccggataa
     2401 agggttgatt ccaagtccgc tcattttaac attggaaaaa gctactgcga atatcagaag
     2461 tagcgctagt caattagggc tgacagtaga tagtcgtatg aagatgttta tacctaaaga
     2521 agaagaaaaa caaaaaagta tatttgataa atttggcggt tagttagaaa ggaggtcatg
     2581 acaatagaat acgattattc agcaattagc gacatttata aagatgacac tttttattat
     2641 gcaaaaaaga ttgttgatga agagattaaa gcaagcaaga aagtgtttaa ggcttgttta
     2701 agacatttga atgacctcaa acgtatagaa gatgaagatt ttaaattcat ctacttgcca
     2761 caaaaagcat ctgatccaat taatttcatt gagattttac cagacgtcaa aacaggaaaa
     2821 ccttatccat tagcaatgtt ccaaaaattt attattggga atttatatgg atggcggaag
     2881 aaaacagacc attccttaag acgttttaga aaagctatga tttctgttgc tcgtaaaaat
     2941 ggtaaaacca ttcttatagc tggtatattg ctttatgaat ttttgtttgg tcacaatccg
     3001 tctatgagta ggcaattgtt ttgtacagcg aacgaccgca cgcaagctaa aatagcttgg
     3061 gatatggcaa aaaagcagtt agcttcctta agagcaaagg atgccgatgt cagaaaagct
     3121 acaaagattg tccgtgatga actaaaaaac ttacatgatg aatcttatat aagggcgctt
     3181 agtcgtgata ctggcgcagt tgatggattt gaaccgtacg ttggagtgtt ggatgagttc
     3241 gcagcgtcaa agacaaacga aatgttagaa ctattggaat ctggtcaagg acagttggat
     3301 aatccgttta tcttaatcat ttcaacggct ggtatggatt tgaatgttcc gatgcacaca
     3361 attgagtatc catacattac taaaatacta gacggagaaa tcacagacga gggctatttt
     3421 ggctatgtcg cagagcaaga caacgaggag gaaattaaag atgaaacgaa ttggataaaa
     3481 tctaatccaa ttctcgaagt cgatactcta catgataagt tgatggacta tctaagaact
     3541 cgtcgtaagg tatctctaga gactggagaa gtcaacaaag tgttgatcaa aaacttcaat
     3601 atgtggcgtc aatccagcga agaatcatat atagataaac agtcgtggga gcttgctaag
     3661 attgataagc cagacacata caagcgtagg gtttggctag gtgttgacgt tgggcgtgta
     3721 agtgacttgt ttgccattag tcctgttgtt atgatggatg attattggta tgttgatagt
     3781 ttttcatttg tagctacaaa gtatggctta actgccaaag aaaagcgaga tggtgtatct
     3841 tatagcaatc tagaacgtca aggatattgc gaaataacaa cccttgagag cggggttata
     3901 gatgatgaac gggttttgga aaaaatagag gagttaatct atataaacga atgggaagta
     3961 catgggattt gctttgaccc ataccaattc ggaacactac ttacaatgat tgaaaaaaga
     4021 catccggaat ggcctctaat agaagtttcg caaacgacaa tggtgttaaa catgccgaca
     4081 aaacaatttc gtgacgacct taaaaaaggc aaaataaagc attctggcaa tccactattg
     4141 accatggctg ctaacaacgc ttatattaaa accgataaca atggcatgag gattgacaag
     4201 aacaagaata gcaacaagat tgacccgcta gacgcagttc ttgacggtta tgctgtatgt
     4261 tacctagaac catttgacgg ttctggctac tggacaagcg agaaaatttt aggaggagag
     4321 acgctatttt gattggtttt attttaaaaa acatacatac attgatcttg ctggtaggtt
     4381 tgggactgtt gatatacggt ctttttttat ttggtgataa ggttggtttt attgctagcg
     4441 gaattatatt agttatttta gctatctatg tagatagtgt agggggaaga aaatgaacaa
     4501 acgtattaag aaaaaacgca aactagaaca ctatatcgct tcactcatcg cagaaaacgt
     4561 tatgttttca aaggaattaa tcaaacaaca tgaacggatt gaacaacttg aaaaaattgt
     4621 agagcataac gcacaagcga ctaacaatga acttagtcgc atcaaaaaac actcaaagaa
     4681 aaaatggaaa aataacgtca ccttacctat agaaaggagg tgagaatcta tgagtttttt
     4741 tcaacctttg ggaacttcaa aggtatcgta tgacgattat atagcgtcag tcttagctgg
     4801 tgacgtctca cagcagtatc taggtgtatc agccttaaaa aatagcgatg tcttgacagc
     4861 tacgtctatt atagctggtg atatagctcg ttttccgctc gttaaaaagg atataaacgg
     4921 ggatattatc cacgatgagg atattaacta cctattaaat gtaaagtcca caaacaacgc
     4981 aagtgctagg acatggaagt ttgccatggc tgttaatgca attttaacag gtaattcgtt
     5041 ttctcgtatc ttgcgagacc ctaaaactgg tcaagcttta caatttcagt tttatagacc
     5101 gtctgaaact actgttgaag aaacagaaaa ccacgaaatc atctatacat ttacggatac
     5161 tttgactgct aaagagataa aatgttttgc acacgatgtt attcactgga aattcttcag
     5221 ccatgacaca atactcggaa ggtctccgct attatcgcta ggcgatgaga tagacttaca
     5281 aacgggtggt atcaatacct taattaaatt cttcaaagat ggtttttctg gtggtatctt
     5341 aactatgaaa ggtgctcaat taagtggaga agcgcgtcaa agagcaagac aagactttga
     5401 gaaaatgcgt gagggatctg ttggagggag tccactagtt tttgacagca caatggaata
     5461 tacgccactt gaaattgata ctaacgtatt gcaattaatc acaagtaata actttagtac
     5521 ggctcaaata gcaaaagcgt tgcgcgtgcc aagttataag ttgggcgtaa acagtcctaa
     5581 tcagtctgta gcgcaattaa tggaagatta cgtaacaaat gacttgccat tctattttga
     5641 cgctataacg agcgaaatag ggcttaaaac gcttaacgat ggagacaggc gactgtatca
     5701 tgttgaattc gatacacgaa gcgtcacggg tcgcaatgtc gatgaaattg tcaaattggt
     5761 taataaccaa attttgacac ctaatcaagg attggttgaa cttggtaagc aaaaatcaaa
     5821 agaccctaac atggataggt accagtctag tctcaattac gtgttcttgg ataaaaaaga
     5881 agaatatcaa gatcaagttg gagtcaaagg gaaaaggggt gaggtaaatg ccaaagaaaa
     5941 taaaccttaa aggtccacta attgctaata attcacaaga agtctatgac tattatggca
     6001 tggaggctgt tagtgctaaa agcattattg acaaattgcc agaagataat agcgacatta
     6061 tcttggaagt taattcaaat ggtggtctcg ttactgttgg tagcgaaata tataccgctc
     6121 tgcgaaatta caaagggaaa gtaaccgcag aaatcacagg aatggctgcg agtgcagcgt
     6181 ctgttgcagt gatgggggct gataaagtcg tcatgagccc aacggctcaa atgatggtac
     6241 ataaagcttt gtttaattgg gtttctggta atagcgacga cttggacaaa gcttctagcg
     6301 ctcttaaatc aagcgataaa gccattgtta atgcttatgt tgcaaaaaca ggtaaatcag
     6361 aagatgaaat catgcaatta atgcggaatg aaacttttat gtcagcacaa gatgctgttg
     6421 agagcggatt tgcagatgaa gtgatgtcgt ttgaagctat agcaagcatt gacaatcaaa
     6481 tgttgccaca agcggttatt gatgactatt acgcaagcag aagcaaacgt aagcaagaga
     6541 ttaataacat gttgctagaa atcgaaaaag aagaaatttt acaagggcta taagctcttt
     6601 ttattggagg aagaaaatgt ttgacgaaaa aatcgaagaa attaaagcgg atatcactga
     6661 attaaacaaa gtgattgctg ctaaaaccgc agaagtcaaa gctgctttag aatcagatga
     6721 cttagaaact gctcgttctg ttaaagcaga ggtagaagct actaaagcga aattagttga
     6781 atcagaaaac gacttgaaat tatatgagtc tagcgttgaa gttggtggtg ctgaaaatat
     6841 tggaggaaaa gaagtgacac aagaggaaaa aacatatcgc gaaagtgtga atgatttcat
     6901 tcgttcgaaa ggtaaaatcg taaacgattc tttacgtttt gaaggcaaag atgaggtact
     6961 tatgcctatt aatgagacaa cccccgtaga acctaaaaaa gacggtgtta agaaaacaga
     7021 tgtaaaacct gtctctagtg aagaaatcct ttacacacca gctcgtgaag ttaagacagt
     7081 tgttgatttg aaacaattca caagcatcca ccaagctaaa aaagcatctg ggaaatggcc
     7141 agttttgcaa cgtgcgactg aaaaaatggt tagcgttgaa gaattagaaa aaaatccaaa
     7201 actaggtaag ccacaattca caaacgtcgc atgggaaatt aaaacttacc gcggagctat
     7261 cccgttgtct caagaatcaa ttgacgatgc agacgtagac ttggttggta ttgttgctga
     7321 gacaatcggt caaatgaaag ttaatacaac aaacgacgct atcgcaacag ttctcaagga
     7381 atttgaagct aagacagtaa aaaacttgga tgaaatcaag aagcttctca acgttgaatt
     7441 agatcctgct tacaatgtgt catttattgt atctcaaagc ttttatcaaa caatggatac
     7501 attaaaagat aagaatggtc gttacctact tcaagattca atcacttctg tttctgggaa
     7561 agtattcctc ggaaaacctg tttttgtgct agctgatgag gttttaggta aagatacagc
     7621 atttgtcgga gatttcaaac gtggtgtatt attcgccgac cgcaaagacc ttggacttcg
     7681 ttgggcagat aacgaaatct acggtcaata cttgcaagca gttcttcgct ttggcgtttc
     7741 aaaagttgat gacaaagctg gctactatgt gacattcaaa ccagaacaat tgccctcata
     7801 aggccactca gggagtggcg caaccagaag taagcagtgc tacagttgtt ggtaaaccaa
     7861 ccaaagacag caccattgaa gatattaaaa aatatctaac aagccaaaat attgacttta
     7921 gcggtaagac gctaaaagca gacttgttaa aactggctgg cgttgaagag gtatagttat
     7981 gactgtatct aaagagctgc tagatagtgt gaaactctat tgtaagatag attttgattt
     8041 tgaagatgac attattgagg aaatgattgc atcagcacaa gaacaaatat gttttgcaat
     8101 agaagaaggc tctactccag aaatgtttaa aaactacgct aagttttcct tagcggttaa
     8161 aaaacaggta aaggaagagt atgatcatcg tgggttatcg gcggatacta atcgttatcc
     8221 gcttgccaac ggtgttttga acattatcca tcaattgaga ttgaggggtg atggggcgtg
     8281 attacacgga aaatgaatgt tagaatagcc atttttagcc aaactggtgg tcagaatgaa
     8341 gatggtgaag ttgtatctgc tatcaggaaa gacttgtata cctgctgggc agaggtgtct
     8401 aaaactcaat tgagagattt taattatcaa tcaagatatc agaatgctag caatctccca
     8461 acaaataaag atacaaaagt atttttaatt agatacaatc ctaagttgtc tattgataat
     8521 acgatgttta ttgagcataa caaacatatc tacaaaatag ataaaattga atttgatgaa
     8581 tccggcaagg aaatgataat attgagtggg gttagcatat catgacaaaa gggcttgatg
     8641 aaattttggc taatattact aagttagaag taaaatcgcc caaaattgcc aaagaagcgg
     8701 taacacaggt tgctgaagaa tttgaaaaag cacttactaa gaatacgcca gtcgattatt
     8761 ctgtgcatga cacaaaactc aaatatgata ctgcaattag tggattcaaa ggtgctaatg
     8821 taggtataac gtcaaaaaaa attggatatg gacgaaaaac gggctggcgt gctcattatc
     8881 caaacaatgg gacgatttac caaaaaggtc aagactttaa agaaaaaacc atcaacgaaa
     8941 tgacccctag agcaaaagaa atttatgcta aaaaaatcaa ggagggacta gggctttgat
     9001 agctgaaaca actgcttaca aattgttaaa aaacgatagg acgcttaatg agctgttgga
     9061 taagcttaga ggcggtcctt ttaaaaacgg atttgatcag ggtatcttta catatgatat
     9121 tcctgacaat cccgttgatt taagaaaagt agaactagcg ccatttatgc gtataaatac
     9181 aacgtatgat ggtccgtccc tatatgcaga tgatgacatg atcagtaatg aacagcgtat
     9241 tacaatcaat ttttggtgta aaaccgcttc tcagtctgac cagattgcca gctgtataga
     9301 taggatttta aaaaatggcg gttttgaaag atacaccgca aacgaaaaac caaggtataa
     9361 agatagcgat attgacttac tcatgaatgt aaggaaatac cgctattttg atttttatta
     9421 agaaaagagg aactaaatgg gaaaagtaaa atttggacta cgtgacttcc agtatgctgt
     9481 gcttggggat aatgataaag ttaatgaaaa agacaaagga atcaaagctc tacccggcat
     9541 gaaatcagct aaacttgata ttacaaacga gcttgtaact gtcatggctg atgatggacc
     9601 atatgttgtt ttatctggtg gtatcacaga aacaaaattg gaaatcgaag tccttgactt
     9661 gacttccgaa gcgcgtcaag atttcttcgg aattaaagct gacaaaggta ttgaaagata
     9721 caataaatcg ctcacaccta acaacgtcgc ttgcatgttt agaacaagtg atgaaaatgg
     9781 gaaagctatc tgggttggtt tgttaaaagg taaatttaac attcctggca tggataccaa
     9841 aacaaaagaa ggtgcgccta acccagaacc agacaaagta acaggtaact ttgttgctcg
     9901 tggaactgat ggtgatgtag ttgtcatcgg tcgtgaagga gtgaatggat ttgagctaaa
     9961 aaaattcaaa gaacttgtgt ttgatggggt gacaggattg gaaaaatcat cagaagacaa
    10021 tagtctaatg taacacttaa ggttgggttt taaatccagc ctttattttt tgataaggag
    10081 caataatgta cgaaattaca cttaaaaaag gcggagtaga taagactttc tcaaaagatt
    10141 ttgtcaatgt cgaagataat atgttggcgg ttgagcatca agttagacag agcgctatct
    10201 ttagtgatga taagcgccgg ttagacgata aagaacatcg caaattaaac gaatcatatt
    10261 tacagatgtt tgtagaaatg tacggaaatc aattcaatgt tgacgatttg aaacagtcta
    10321 atgtgtctgt tttggacaaa ttaactgagc tttttgttga tgctttaggc ggagagaaag
    10381 aggaagacga aaaaaaggaa caataacgcc tcaagaagct aaagacaacc tgctcttgtg
    10441 gattcaaagt ttactaaaaa atgggtatac cattttagat attaaaaaaa tgcgcttatc
    10501 agacatagag ttgatggtaa aagcgctaga aactgatttt gctgaaaaag aagaagtggt
    10561 tgaaacaact ttagacaaag cgttcccatt ccttttcggc tagaaaggag aattaatggg
    10621 gaatataggt gatttagtag cgactgctac actagacata tcacctttta tgtctaatac
    10681 aagaaattta aaaacttata tgaagacctt agataactct ctaaaggctg tagaaaatag
    10741 ttttaatggt catggtagta gagttaaagg tttaaaagct atctacacag aaactggcgg
    10801 tgcattaaaa agttatcaag aattactaaa aaaacaatct caaaaatata atgagttaaa
    10861 agaaaatatt ggcgatgtca acaaggcgac agcaagccaa aaacaagcac ttattaatgc
    10921 taagtcagcg atgatggaaa cagctgctaa agtttcagaa ttgcaaagta ggctcaaagc
    10981 tttaggcgct gaaacaagtg tttttactcg ttttggtaac gctgcggagc aagctgggaa
    11041 aaaaatgaag tcatttggcg attctgtatc tggtgttggt gctgctttta caaaaggagt
    11101 aactgcacca attgttgctg gcgctggata tgctcttaag gctgctgttg attatgaaga
    11161 tgcttttgct ggcgtcaaaa agacagtaga cgaagtaaaa gactcaaatg gtaaagttgt
    11221 ctactcttac gatatgttgt ccaaaggaat tagacaaatg gctaaagaga taccagcttc
    11281 tacaactgaa atttctcacg ttgctgaagc agctggtcaa ttaggtatca agacaaagga
    11341 tgtcttagga tttactcgtg tcatgattga tatggggaaa tcgactaact tatcatcaga
    11401 agaagcggca actgcactag ctcgttttgc taatatcacg cagttggacc catcaaaata
    11461 cagtaatcta ggaagttcta tcgttgagtt aggaaataac tttgcgacaa ctgaaaaaga
    11521 aattgttgaa atgggtcttc gcttagctgg cactggtaaa gttgtaggat tgacagaccc
    11581 tcaaatatta ggtcttgcaa ctgctatgag ttcggttggt atcgaggcag aggctggtgg
    11641 ttcggcgttt agtcgtgtca tgcaaaaaat aaacacacag gttctttctg gtggagaaga
    11701 tttatggaag tttgcgaaaa tagccggcaa gtctgctgat gaattcgctg cgtcatggaa
    11761 gaaaaatcca caagaagcga ttattgattt tgtaaaaggt cttaaacgct ttaaagaaga
    11821 agggaaagac gtaactgctc acttgcaaga catggatatc aattctgtac gtgaaattga
    11881 tacattacaa cgtttggcag gagctggcga cttgctaggt gatgctttca agtctgctaa
    11941 taaaggtttt agcgaaaaca aagctttaat gaacgaagcg aacaaacgtt atgaaacgac
    12001 agcatcgaaa ttacaaatgc taaaaaatca agttaatgat gtcgctattg aatttggtgg
    12061 acctcttatt gatgcactgc gtgacggttt agaagctggt aaacctatta ttaaaggcgt
    12121 tgcagactta gcaaaagcat ttagttctct ggataaagag caacaacaac aaatcataaa
    12181 atggggacta attgcagcag caactggacc cgcattgtca atcttcggca aaggcgttgg
    12241 cgtaattggt ggaacgattc aaggactagg aaaattaagc aaaacgttag gcacactctc
    12301 cgggagtctg ggagcagcaa aaactggagc tgctgcttta ggagttggag cggaaggtgc
    12361 aactactgca ttaactggaa tgagtggagc ggcagcattg ctagggaatc ccgtaacttg
    12421 gggtgttgtg ctaggaggag ctgctgcgct tgctgtcgga tactttgctc aaaaagccta
    12481 cgaagcgcac caacgtacgc aagagtgggg gactaaagtt agtcaagtac aagccaatga
    12541 actgcaatcc tttaaagata aggttgatca aacaaatcaa tcaatggaag gctttagagg
    12601 gggagctgaa caggttaact ccgtgaagac agcgttccaa ggtttagttg ctgaaattga
    12661 aaagctagaa aataaagatt taagcgaaaa aatcaaactt gcagaacaat ttggttttag
    12721 ccaaggtacg atggaccaaa taaaaaaatc tagccgacaa actgtagaga atgttaaaca
    12781 gatgtctgat gaagttatca atatctatca aaatgctagt aatgaacaca gaagattaac
    12841 cgaagaagaa aaagctgttg tattagcgaa tcaaaacgag cttatcaatg tgcaactgtc
    12901 aaaattgaac tactctgcta aagagaaaaa agctatcgta aaagcaatga atggcgattt
    12961 aaattcattg aatagccagc aattaaccaa agctcttgaa gttactgaaa aatggataaa
    13021 agcagaaaat aagtcataca ataaactcaa aagtggtctt aaaaaagttt acgactctat
    13081 aaaaggtaat gacaaagagg ctgtcaaaac tcgcgaagag atacacaaga agcaacaaca
    13141 acttgaagca gaccattact taaaaatgga agcttacggc aagcgctacg ctgcaattca
    13201 aaagaaattg ctaaaaggta ctgctaaata tttagaccct caattgcaac aagcgatggt
    13261 taatgatgtt aaaaagcaaa tgaaggagct tggtttgtct tacgaagagt tgatgacaaa
    13321 gacaaccaaa gcggcttcaa aagcgcaaga agtgaataca atgtgggcta gaaccactaa
    13381 gaaatctaca gaagaccaaa agcttgctaa ttcgcagtgg aacggacttg tctggaatcc
    13441 caaaacaggt aagttaaaaa ccaatgctaa agaggaagta gccaaggctc ttgaagcgga
    13501 aggtggctgg gacagactta agtttattgc taaaaacgca aacctagaaa caaacgctcg
    13561 gataaccatg gcagaagtac ttgtagagac tggcaaatgg gataccctta ctccagaaga
    13621 gaaagagtta gttgttgatg ggcatcaagg tattcaagct atagtagaaa gtaagcaaca
    13681 tttagaaata tggaatagtt tgccagaaga agttaagcgg attttagggg ataacaaaga
    13741 cttcctgaat aaaaaaggag ttgcaacacg aactcttgaa aattggaatg ctttaaaacc
    13801 agacgaaaaa aaattactcg ctaaaaactt aacaaaaaaa gggaaagatg aagcacaaaa
    13861 aacgatcaat agcttagtcg gaaaagaagt caaagtgaca gcggctaata aaacattgtc
    13921 cggagttaac agcgctcaaa gaacattaaa tagtgtgcaa gataaacatg tcactatttg
    13981 ggcgtcaatc aaaaaaactg cgagtgactt atggagcaaa ctaacaggat acgctgttgg
    14041 tacagactac caccctggtg gacttgcgat ggtcaacgac caaaaaggtt ctttatataa
    14101 agagttagta acattaccaa acggacaatc ttttatccca gatggacgtg acgttatctt
    14161 gccactccct cgtggttcga aagtcatgaa agcaagtgct actcgtgatt acatgtatga
    14221 cttaggaata ccaaaatatg caaacggtat tggttttgac aacactaaga ttgctaatat
    14281 cacgcaacgg atgagcgaac ttcctaaaaa cacagtgact tcaacaacag atgataagtt
    14341 gtattggatg attgaggaaa tgattgcagt tttaaaatct actaaagata acagcgttat
    14401 tgagcaagct ttagacattg cagaacaagc aatagagcgt ccagtagaac tttacttaca
    14461 ggacggtcag tgggtagcta aagttgcgga ccgcattaca aattatcaaa cgcaacgaaa
    14521 tagtcgcaat aatcgaatga aaggaatgct aaattgaaat ttatttatga cggtgtagac
    14581 atgtctaaat tttttaggat atctcgtgtt gagcggtcta taggaaatga acgaacattg
    14641 tcgttgaatg agacgtttca acttggtaca gctattcgtg gcataaagac gggcgctaaa
    14701 attatcaaag tacatataga gccactagaa ataaatggcg ttttaaccga gcaattaaaa
    14761 catgaacttg ctggtgtgtt aaatgttgac aagcctaaaa aaatgacgtt cggagatgaa
    14821 ccagataagt actatctcgg gcttgcacaa ggtgagattt cgacagaaaa agtagcaagg
    14881 tggtatcaga gggctgttat cactttttac atacccgacg gtgttgcaca ctctatcact
    14941 tataaaaagt tttcggacta tacgcaagaa ggcaacaagc ttatttttaa tttgcaaaat
    15001 gatggtaata ctaacgctta tccaataatc aaaattaaac ataactctga aaacggttat
    15061 atcggtatcg caaatgaaac gggcgctttt gcacttggat catcggagga agaagatggg
    15121 actatcgttc atcgcaacga atcccttttt gattactcaa aagctattgc acaagcttta
    15181 gaaggtgcgc ccaacgtcgc aaaactcaac tatatgccac caacatatga cacagaactt
    15241 aaacggatgc gcattgataa tatcttaggc tctggtaaag gcggtgaata tgttgttatt
    15301 ggaaatagag ggactacccc gggctacacc gagcacattg ggactcgaac gtttattatc
    15361 aatcctgatt caaacggaga atacactctc aatgagcacc tatggtggca acagattttt
    15421 attgctactg cgcaggatca gaaagggttt ttaaagcttt gtgtaacggg aatcgacgat
    15481 gaaggaaatg acgagttttt gtatggaatc gaaacttaca aacgaaaaaa tggttttgaa
    15541 acagaataca atttttttgc tcttgatgat gacggtgtag gctggagatt ttataagcag
    15601 tttaaattcc aagcagacag aaattatcac aatccttttt caatgaatag aagtagagcg
    15661 gttgagattt tcagggaaga agacaagttt cgtatttatt ttaacggtgc gcatcatcat
    15721 gtaactgttc catctcttaa aggaaaaaaa tctcgcaaga tacatcttgc aatggggaca
    15781 tgtagtgata gctctaaata tatcaactac aatcttttcg aaaaggtcaa ctttgaaaaa
    15841 atgggagtgt ctcactacaa caacattgtt aacaagtatc aaccaggcga tgaagtggtt
    15901 atcaattttg aaaatgacac agtcaaaaca aaagacttag attctattca agacatggtt
    15961 ttaggttcgc agcctatatc tataccaccc ggaaaaacag agttcgtcat gcagctatct
    16021 aaattttctc aatatgcgcc aaacgttgaa atattaatgg aagagaggtg gttataataa
    16081 cgctagtaat acatgattct aagttgcacc ctgttttgtt gctagacaat gaaaaacaag
    16141 gtacgcttaa ttattttgac gacacatgga ctagacagtt aactacaggc tcatctgttt
    16201 ttgagttttc tgtgtacaaa aaaacacttg aaggagacaa cccactcaat cataagtatc
    16261 aagtgcttaa tgaccaagcg tttgtatcgt ttgtacataa agataaagta cagctcttta
    16321 atatcatgca gatcgaagag actgaaacga ctgtacgttg ctattgcgaa aatcttaatt
    16381 tagagttact aaacgagtat tgcaacgcat ataaagcaac taaagcaatg tcatttgaag
    16441 agtatcttgt gcagtttgat attttaaatt ggggtgcttt gacaattggc acaaacgaag
    16501 ttaaggacaa aaaactgaca ttggaatgga ctggtcaaga cactaagtta gctcgtattt
    16561 tgtcaattgc taataatttt gatgcagaaa tcgaatttga aactcaatta cacaacaatc
    16621 acacgtttaa agcgtttatt gtaaatgtgt acaaggaata cgaagagggc gtgtcctatg
    16681 gcgtaggtcg tgaccgcagc gacatagtgt tgagatatca aaaaaatgta actggtatca
    16741 ctaaaaaatt agacaagcgc cagatttaca acgccatacg cccgtatggc aaaaagacag
    16801 tcaaaggcga gcgcgttatt tctaatcctg taactcgcaa agtcactaaa acagttgggt
    16861 caaatcgtac atatttaggc ggagacctca aatattatgg tcatacaatc aaaaaagcta
    16921 acgtacaatc tattattaac tacgcggtgc agtataatat tttgccgagt ggaatcatat
    16981 gtcaactgta cttagaaagt ctttggggtg attcagcagt tggtaaacgt gacaataact
    17041 gggcaggtat aagcggcgga gcacagacac gccctagtgg agtaaaagtc actactggaa
    17101 tggctcgtcc tcccagcgag ggtggaacat acatgcacta cgcaagtgtt gatgactttt
    17161 taaaagatta tacttatctt ttagctaaac aaggactata taacgttgtt ggcaaaaaga
    17221 atatagcaga ctatacaaaa ggtttgtttc gtgtcggtgg cgctaaagat gattacgcgg
    17281 cagcaggata tcaaagctac acaaatttga tgactaatat ccgaaatggt atcaataaag
    17341 taactggaaa tatcctcaat acgattgata agctgtggca aacaccagta cagcctataa
    17401 cagccgtaaa cgtagctaga agagctacta agacaatgca agcactaaat gaagctacta
    17461 gacttaaagg tcgcagaatc ggctcaggac agtgttatgc tttgtctggt tggtacgcta
    17521 agaagttaga cggagcttgg attgatagtt ccatcggtgg tatccgtggt cgcattggcg
    17581 gtgggatggc tgctgcttta atcggtactg actataactg gggggcgtat ggttggaagc
    17641 tagacaggtc gcctaatgct ggcaacttgc aagctggcgg tatctataat gttaaagcaa
    17701 attttggtgc tccgttttat acaacacaat gggggcacac agggattatc aagagtgtgt
    17761 ccaagaccag agttactgtt ttggagcaaa actttgttgg ccgcatgtat gttgtcgaaa
    17821 actcatatga cattaactct ttcgcatctg gattgcagac agtatgctat cctcgtgaga
    17881 ttgcacaagg aatggctgtc aacggtgcaa caacacagca agttagtggt gggacacaga
    17941 tatcatatga ggaagtcgta caagaagcac agacagaatc atacgaagaa gaacaaatca
    18001 tctacattga caactcaatc tacaaagagt ggaaagatga aaacggtaaa gtagagtact
    18061 atctcaaaaa tggatttttg tacgcacctt tatccagaga ccgttatcca tctgttttaa
    18121 caggtaatga gacacgggat aactggatac gaaaggacat ggaaattgag actgatagtc
    18181 aagacgtctt gatgtcaaca ggtctaaaag acttaaaagc acacgcctat ccagcaatca
    18241 cttatgaagt tgatggctat gttgatttag aaattggcga tgtcgtacgc attcaagatg
    18301 atggttatca accacctttg attttgactg ctcgtgttat tgagcaggtt ttgtctaaag
    18361 ataatccaaa ctcaaacaaa actaaattta gtaattttgt tgagaaagaa agtcaactag
    18421 cgtctgattt aatcagcgat atgttgcgtc tatacgatga gtcaattccg tatgaaatta
    18481 aactagcgac ttcgaacggt gtcgctttta aaaatggcac tggtgaatct gtcttaactc
    18541 caagtttgca aaaaaacgga aaagactatg aagctgttta tttttacaaa aatggcgact
    18601 cattaattga tatcggacca tcactaattg ttaaagcaag cgactttaac cacgttttaa
    18661 acgtaacagt agaggcgtat ttaaatgagg agcttgtggc aagtacacaa atatcattta
    18721 cagacacaga agacggtgca gacgggaata cgccttactg gcatgttgct tatgcaaata
    18781 gcgcagatgg gacagttgat tttagcgtgt ctgatagcgc aaacaagcgc tacattgggc
    18841 aatatactga ctacgatgca ataggctcaa acgaccctaa aaaatataag tggactgata
    18901 tggttgggac ggtcgtcgtt gggacaaata atctaatcga tggcactaag tcgtttagcg
    18961 gagaagactg gtttacttct gcaacactag aagatgaaaa tatctctaat tatccattta
    19021 catttaaaaa atggataagc ggtcaaaagg tatcacacgc aaaagacatt atggttgagc
    19081 aaggtgtgac atacactttt agtgcttatg ttaaacgtga ggtagctggg aatttatatt
    19141 tttatcttta tgatatagca gatggtttta ttactagcga taccccacga gagacaatta
    19201 taaaaaacgt tgactctagt ctcagacgtt ttgaaatcac ttttacacca actaagacag
    19261 gtaggattag accaaggttc gcgatggtgt catcggagca aggtagtttc agctctggtg
    19321 ggtttatgct cgttagggga aataaaacag gcgactggca ggaatcagaa gctgacaaag
    19381 ctagtaattt agatgcaaaa gccgatggtg cttttacagt tgagcaacta aatgcactcg
    19441 cagaacgtgc tcgcattgca gaagctgaat tgcaatctaa agcgacacta gacacagtaa
    19501 acgactgggt taaagcacta caagacgaaa tcaaagcacg agaagaagga caaaagctat
    19561 cagaacaaaa actgatagac tcttctaatc gcatgatagc agtacagcaa acgattggtg
    19621 aaatgcaggt tcgcaccgat tttgtcaata aatttatgag tcagtcggaa gatgggcttg
    19681 ttattggtca aaaagacggt acttctagtg ttcgtgttga taacgaccgt ataagctttt
    19741 actcaagtgg taaagaagtc gcttatattg ctcaaagtgt gttagttatt gatagtggta
    19801 tctttacgac caaactacaa atagggcgct atcgtattga gcaatacgag ctaaatccag
    19861 atataaatgt tgtccgctat gttggatagg aaggaggtta aatgacaact tattatagta
    19921 actctgatag gagttaccgc ttaacttata ttgttgacga ggtctcaaca tcaatcgcag
    19981 ataatagcag tcaagttaga tttagacttt atttaacatc aaggactaac agttacgcac
    20041 aatacaactt tagcggatat gcttgggttg gtggtagata tgactttagc gccccatctg
    20101 cgcttggctt taacagtaat tatttactga ttgataaaac tatcaaagta ccacacgatg
    20161 ctgacggtag taagactgtg gttgtcgcag ctaagttgtc aggtcctggc gggtatgcac
    20221 ccggaacgct aaatatacca gaccaaaaat ttacgctgac taaaatccca cgaacaagca
    20281 cagttgctgt aagtagtggt tattttggtg atacgctaaa tgtcaacatc aataaaacaa
    20341 atgacagctt tacatacgat gtcagatata acgttaacgg gataactggt actgtggcta
    20401 gtgatatatc aggatcaacg acttttaaaa ctagtttgga ttgggctagt acgattccaa
    20461 atacaactag tacaccagct acaatttacg ttgacactaa atcgaatggg tcggtcattg
    20521 ggacatcaac aggtattttt tatctaactg tacctgataa catcaagcct aaaatttcca
    20581 gcctcacttt atcagacacg aatcaaaaag catctacaat tgtaggtgct aataattttg
    20641 tccagataat ttctaatcca gtcgtcacat ttaatggggc tagtggtgct tatggctcta
    20701 caatccaaaa ttttaacgct gaaattatag gtaaaaacca atctacgcag tcaaacggtg
    20761 ggtcattagg cattttaaac tttagtggta aagccacagt taaagcaaca gtaacggaca
    20821 gtcgcgggag ggtgtcagac cctgtgacaa cagaaatcaa cgttatacca tattccccac
    20881 cagcttttag ttttactgta acacgcgcag gcgctaaaaa tgaccaacta gttgtcactc
    20941 gtaacgctaa aatctccccg ctgattgtcg atggcgtcca aaaaaataaa atgacgctga
    21001 cttttaaaac agcaccactt aataccacga gttttacaat agacacatca aacgcaagcg
    21061 gtacttatac tacagtcgca gagttgatta actcaacagc tacacttagt ggttcgtatg
    21121 gagctaacaa atcatttgat gtctacggtt tgcttagtga tgtttttagc gcaagtggag
    21181 gcggtacacc tgttaaacaa acggtatcaa cagagtcttt cccgctgtca tggcataaaa
    21241 acagcgtcgg aattggaaca ttaccaaaaa ttgatgatac aggttcttta aatgtcgcag
    21301 gcaatatcta ttctgatggc aagcaaatcc aacaaaaaca acttgcttta aataatggtg
    21361 gggcatttag gcatgataca acagacttaa acagcttgca agacacaggt ttttattgtg
    21421 tttttaaagg cgataacaga ccaagtggtg ctggaccggg ctatctaaca gttgtaagac
    21481 acgagacagc caattacgct taccagcatt tttatgaccg cacgaacaaa accattttta
    21541 cacgagtgct agaaaacggg gcatggagtg gttggagtga gtacgctaaa aaagatagct
    21601 taccgcaatc cgcaccagcg gtagaagata ctggttggca atacatcggc aacggtttta
    21661 attacaggaa aattggtagc atggtcacta ttaaatatga ctttgcaaca aatggaataa
    21721 accagtttac ggtcggttcc atgccaacga atttaattcc aaacgaaatg atgtttgcgg
    21781 ttactgcgtg gactgtgcaa ttaaatgtat taaatgtaca agttagtgca gatggtcgta
    21841 ttttatggtt caacccatca aaatgggcgg ttaatgttaa aggacaaatt aattggataa
    21901 tttaaaagga ggaattatgc ttgaattttt gaatagatac ccagttttac tggaagataa
    21961 aagtgtaaaa gagactaaag cgattttagc atttacgtct agcacgatta aagcaaattt
    22021 tgaagtgacg ctaccagcag aagaaaatga taaaaaattt gctgaaactt taaaaacgtg
    22081 tgaaaagctt atctttgagc aactttacaa agacaaagca gaagcagaac aatttgaaaa
    22141 aattaatgac gcaattgcta agtcaaaggc gcaatcagat aaagcggaaa atatgattaa
    22201 actgatgtca gcaactgtta acgatttgat taagacaatg gctgacggag ggaaattgaa
    22261 tgatacaacg cttaacaacg ctagcgaaaa tagcagtaca catatttaaa aacaaaaaag
    22321 gagaaaaaac aatgatgatt aattactttg caatgcagat tgaactaggg tggattacta
    22381 ttgatgacgt tccagcattt tgtcgtgagc gagtacgtaa actaattgaa gtttctacgg
    22441 ttggtacaga aggaaaatga ggcaatgaat gaacattgac atactacaaa ttggcgcagc
    22501 aagcggggcg attttatcgg tagttggatt gtgggcgttt gttgttaatc cgtttaaaac
    22561 agcgatgcaa aaaaacgaag atacaatgag cgcccttaaa gacacaataa aagaactggc
    22621 ttacgaacta aaagactcac agcgtgacag ggaaaagata cataaaatct tggatatcca
    22681 cgagcaacga ctcggaaaaa cagaagacga catcattgtc aacaaggaac aaataaaaac
    22741 attatttaat aggagaaata aatatgatta atttaaaatt acgactacaa aacaaagtaa
    22801 ctttgatggc tattttagga gctatatttt tgctagcaca gcaagtaggt attaaactac
    22861 catcaaatat tgcggatatt gccaacacag cagtaacgct tttggttttg cttggagttg
    22921 tgacagaccc aacaacaaaa gggctatcag atagcgaaca agcattgaat taccacgagc
    22981 caaaaaaata ggaggggaca tgcgagcaat cactaaaata gcaatggtac tagcgatagc
    23041 aatactgtac ataccgcttg cagtgattgc tttttttatt tatccgattt atttgatttt
    23101 tgaaaaggag gagtaaatgg caacgtatca agaatataaa aacaggtcaa atggtaacgc
    23161 ttatgacatt gatgggtcgt ttagtgcaca atgttgggac ggttatgctg attactgtag
    23221 atttttaggc gtaccgtacg caaactgtac aaacacagga tacgcaagag acatttggga
    23281 acaacggcac gaaaatggca ttttaaacta ctttgacgag gtagagacta tgcaagctgg
    23341 ggatgttgct atttttatgg tcgttgacgg tgtgacacct tatagccatg tcgctatttt
    23401 tgatagtgat gctggtggtg gctacggatg gttttttggt caaaatcaag gtggaacaaa
    23461 tggagcttat aatctagtaa aaataccata ttcagcaact tatcctactg cgtttagacc
    23521 aaaagttttt aaaaacgctg ttactgtaac tgataatacc ggtttaaata aaggtgatta
    23581 ctttatcgat gtatcggctt atcagcaagc ggatttaact gctacttgtc agcaggcagg
    23641 cactacaaaa acgattatca aggtatcaga gtctattgcg tggctatctg acagacacca
    23701 acaacaagct aacacgagtg acccgattgg ctattatcat tttgggcgtt ttggcggtga
    23761 tagtgcttta gcgcaaaggg aagcagactt gtttttgtct aacttaccag gcaaaaaagt
    23821 atcatactta gtcattgact acgaagattc cgcaagcgca gacaagcaag ctaacacaaa
    23881 cgcagttatt gcatttatgg ataaaatcgc tagcgctggt tacaagcctg tttattacag
    23941 ctataagcca tttacactta ataatgttga ttatcagcag attattgcta aataccccaa
    24001 cagtatttgg gtcgcaggct atcccgatta tgaggtgcgc tctgaaccgc tgtgggacta
    24061 ttttccgtca atggacggtg tgcggtggtg gcaatttaca agtgtcggaa ttgctggggg
    24121 gctagataaa aatgtcgttt tattagctga cgacaatagc caagtgactg taccaaaagc
    24181 agataaaccg caagaagcag ttagctttaa tcagcgttta gatgttaata ctaagctaga
    24241 taactcaaat acgccatact acgaagcaac ccttagcaca gactattatg tagagtctaa
    24301 gccaaacgca agtagttctg ataaagaatt tatcaaagca ggaactcgcg taagagtcta
    24361 cgaaaaagta aatggatggt cacgtattaa cgcttctcag tctgaccaat gggtcgaaga
    24421 taagtattta gctaatgcca cacaagtata aaacaggagg taaaattcct ttagataaga
    24481 caaatgccct cgcatacgcg cagacatgtt ctgatggggg gtttttttgc taaaaaattg
    24541 agggtgtttc atggcggaaa attttcaaca caaaagttac agagaacaag ttgaactttt
    24601 ggaatctaga ggtattgtct ttagtggaag aaaagctaaa agcaaaagtt gaacaagcgt
    24661 caggcagtct taaagaaggt gcagggaagc taaccggtga taaagagtta gaagcaaaag
    24721 gatttgtcga aaaaacaatt gctaaaggta aagaactagc agatgatgct aaagatgctg
    24781 ttgaagaggc agtagatgct gtcaaagaaa aactgaaata aatattaacc gctcattaaa
    24841 tgagcggtta
//
LOCUS       DATRME010000023        24429 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRME010000023 DATRME010000000
VERSION     DATRME010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24429
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBC23_000928"
     CDS             complement(34..744)
                     /locus_tag="VBC23_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6842890.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBC23_000929"
     CDS             complement(741..1439)
                     /locus_tag="VBC23_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6842891.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBC23_000930"
     CDS             1607..2371
                     /locus_tag="VBC23_000930"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6842892.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIVHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBC23_000931"
     CDS             2479..4986
                     /locus_tag="VBC23_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6842893.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBC23_000932"
     CDS             5072..6265
                     /locus_tag="VBC23_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6842894.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBC23_000933"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBC23_000933"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6842895.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBC23_000934"
     CDS             complement(7672..8229)
                     /locus_tag="VBC23_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6842896.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBC23_000935"
     CDS             complement(8226..9209)
                     /locus_tag="VBC23_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6842897.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBC23_000936"
     CDS             complement(9455..9868)
                     /locus_tag="VBC23_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6842898.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC23_000937"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC23_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6842899.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBC23_000938"
     CDS             10909..11883
                     /locus_tag="VBC23_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6842900.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBC23_000939"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBC23_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6842901.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBC23_000940"
     CDS             12806..14203
                     /locus_tag="VBC23_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6842902.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBC23_000941"
     CDS             14345..15865
                     /locus_tag="VBC23_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6842903.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBC23_000942"
     CDS             complement(15978..16238)
                     /locus_tag="VBC23_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6842904.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBC23_000943"
     CDS             complement(16347..17282)
                     /locus_tag="VBC23_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6842905.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            17549..18571
                     /locus_tag="VBC23_000944"
     CDS             17549..18571
                     /locus_tag="VBC23_000944"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6842906.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            18630..19073
                     /locus_tag="VBC23_000945"
     CDS             18630..19073
                     /locus_tag="VBC23_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6842907.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            19150..19425
                     /locus_tag="VBC23_000946"
     CDS             19150..19425
                     /locus_tag="VBC23_000946"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6842908.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            19418..20614
                     /locus_tag="VBC23_000947"
     CDS             19418..20614
                     /locus_tag="VBC23_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6842909.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            21198..21545
                     /gene="rplS"
                     /locus_tag="VBC23_000948"
     CDS             21198..21545
                     /gene="rplS"
                     /locus_tag="VBC23_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6842910.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            21656..21727
                     /locus_tag="VBC23_000949"
     tRNA            21656..21727
                     /locus_tag="VBC23_000949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21689..21691,aa:Arg,seq:cct)
     gene            complement(21690..22304)
                     /locus_tag="VBC23_000950"
     CDS             complement(21690..22304)
                     /locus_tag="VBC23_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6842911.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(22307..22573)
                     /locus_tag="VBC23_000951"
     CDS             complement(22307..22573)
                     /locus_tag="VBC23_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6842912.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            22573..22977
                     /locus_tag="VBC23_000952"
     CDS             22573..22977
                     /locus_tag="VBC23_000952"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6842913.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            23139..23339
                     /locus_tag="VBC23_000953"
     CDS             23139..23339
                     /locus_tag="VBC23_000953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6842914.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            23361..23621
                     /locus_tag="VBC23_000954"
     CDS             23361..23621
                     /locus_tag="VBC23_000954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6842915.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            23747..23956
                     /locus_tag="VBC23_000955"
     CDS             23747..23956
                     /locus_tag="VBC23_000955"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6842916.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(24129..24290)
                     /locus_tag="VBC23_000956"
     CDS             complement(24129..24290)
                     /locus_tag="VBC23_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6842917.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYENIIV"
BASE COUNT         7886 a         3878 c         4857 g         7808 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcacct gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg tccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caaggctata ttgttgacta
    17341 tattatagct taaatctttt attgaggaaa gaaattaaga tagcggccgc aatgtttgtt
    17401 tatttttata tgatggtatg ttattttttt tataaaattc ggatactgct aactaacaat
    17461 cttttttatg ataatatttt gtaaatgtat tgctttttag ctaacttttg ttaatatagt
    17521 aaaaatgctt tttaatagga gataaattat gacacaagca gtattaaaag aattggctaa
    17581 ggcggagttg cattgtcatc tagatggctc actttcttta ccagctatta gaaaattggc
    17641 taatatggct gatataatac tgccaaatag tgataaagaa ttaagaaaat atgttatagc
    17701 tccagctcaa acagaaagct tggtagatta ccttaaaacg tttgagttta tcagaccgct
    17761 tcttcaaacg aaggaagcat tacgttttgc tgcttatgat gttgctagac aggcagcttt
    17821 agaaaatgtt atttatattg aaattcgttt tgcgccagaa ctctctatgg ataaaggttt
    17881 aaccgcaagt gatacagtct ttgcagtctt agaaggttta gcagatgctc agaaagaatt
    17941 taatatcgtt gcacgcgccc ttgtctgcgg tatgcgccaa tcgtctcata aaacgaccaa
    18001 ggaaattatt aaacacattg ttgatttggc tcccaaaggc ctggtaggtt ttgattttgc
    18061 aggtgatgaa ttttcctatc caacagatag tttagtagat cttattcagg aagtaaaacg
    18121 ctcaggttac ccgatgactt tacatgcagg tgagtgtggt tgtgctaagc atattgcgga
    18181 tagtttaaat ttagggatta agcgtatggg acatgtgaca gctttaacag gacagagggc
    18241 tttaataaag cgatttgtcg aagaagatgc tgtagcagag atgtgtttaa ctagcaattt
    18301 acaaacgaaa gcagcctcaa gtattcaaag ttttccttat caagaacttt atgatgctgg
    18361 aggtaagatt acaattaata cagataatcg tactgtttcc gatacaaatt tgacgaaaga
    18421 atatagtctt ttcgtaactt attttggaac gaaaatagaa gactttcttg tttttaatca
    18481 aaacgctgtg aaggcctcct ttacaagtga ttcagagaaa gatactttac tacataagct
    18541 acaagaaaac tatgacagct atctaaaata accttcacga ttctttaaaa atatgataga
    18601 atagtggtaa tttacaagtg gaggaagata tggctttagc aaagattgtt tatgctagca
    18661 tgaccggaaa tactgaagaa attgctgaca ttgttgctga taaattacgt gacttgggtc
    18721 tagatgttga agtagaagag tgtacgatgg ttgatgctgc agattttgaa gatgctgaca
    18781 ttgcaattgt tgccacttat acatatggtg atggagattt accagatgaa atcgtggact
    18841 tttatgaaga tttagcagaa gttgatttat ctggtaaagt ttacggcgta gtgggttcag
    18901 gtgatacttt ctatgattac ttctgcaaat ctgttgatga gttcgaggct caattcgcat
    18961 taacaggagc acaaaaaggt gctgattgtg ttaaagttga tttagcggca gaagacgaag
    19021 acattgaaaa cttagaagct tttgctgaag aaattgcttc taaacttaac taagctacca
    19081 aaaagacgaa cacgagtcgt ctttttaact tttttaccat cttttgtaac tagaatatga
    19141 gatatagata tggaattaga agagattcgt caagaaatag atgaaataga tcagcaattg
    19201 gtaagccttt tagaaactag aatgggtcta attttagagg tcatagcatt taaaaagaaa
    19261 caccgacttc cagttttaga taataatcgt gaaaacgaag tgcttaacaa tgtattgaag
    19321 aaggtccaaa atcatcaatt tgacgatgtg atacgagcaa ctttcaaaga cattatgaca
    19381 gaatctcgtg tttatcaaaa ggagaatatt gtagacggtg attaaacgat ttgataatta
    19441 tgctcagtac gtacttgttt tagggtttag cttactaatc ggtggtgtgg ttggggctat
    19501 tacagcagtc tttggacgtg ttctattatt cctgacagcc tttagaagtg attatatagc
    19561 ttacctactc ccttttctta gcattgttgg tttatttata gtttttgtct atcaaaaatt
    19621 tgggggtaag agtgttaaag ggatgggatt ggtatttgaa gtaggtcatg gtaatgagga
    19681 gactattccg aaacgtcttg ttcctctcgt gatattaaca acttggttaa cccacttatt
    19741 tggtggtagt gcaggtcgtg agggggttgc tgtacagatt ggggctaccg tttcacacta
    19801 ctttcaaaaa tattgccgtt tacaaaacgc ttctcaactt ttcctagtaa tgggaatggc
    19861 agcaggtttt gctggtttat ttcagactcc tttagctgct actttttttg cgatcgaagt
    19921 tttggtagta ggtcgtttaa tggtttcgta tatattacca agtttaattg ctgctctgac
    19981 agcaaatttc gtctcacatt ctttaggttt agaaaaattt tctcatagta tagcaacatc
    20041 aatggcgtta acaccagata ttattctaaa attattggtt ttaggactat gttttggtct
    20101 gtgtggcaat ttatttgctt atttattggc taaagctaaa ttaattgctt ctagtaggtt
    20161 actaaaccca tataaacgga ttttcacact tggattgtta gctacttttt tattgtttat
    20221 cttccatttt ggacgatatt caggactggg aacaaatcta attgaggcta gcttcactaa
    20281 taagaatctt tatgattatg attggttgtt aaaattgtgt ttgacagtta ttactttggc
    20341 tgcaggttat cagggaggag aagtaactcc cttatttgca ataggagcaa gtttaggagt
    20401 gattattgcg cccattttag gtttgccagt tattttagtt gctgctttag gctatactag
    20461 cgtttttggg agtgcaacga atactctgtt aggtcctatc ttaataggtg gcgaagtctt
    20521 tggctttgcc aacactcctt attttgttat tgtatgtctt gttgcttact ccatatcaca
    20581 tgctcatacg atttatggtg ctcaatctag atagttagtg atgagtttta aaagagttta
    20641 tcagtttata gaacggaata ttccgttctc ttttttattc tcttaaagtt tataaaaatg
    20701 aagtttgtaa attttatgaa tgcaaaaatt atgtggctaa tataattttc ttcaaagaaa
    20761 ttgattttat gattgtcatt aaaatactgt aaaatccaat tgaatgtgta agaacttagg
    20821 ttaggataat gtctacaagc atttaagtcg cgatggtgaa atgcattaga agataaaaag
    20881 ttgatgtgga tttcaacttt atgggaaaga gtcaggtgac acgaaagcta atatagaaaa
    20941 cgtagtttaa aaaatcatat aatattaaat gaattaaact ctcaaaaatc agaaaaaagc
    21001 taacctagaa acagtaaatc aaatttcaat aatatacaaa taactattct aaaggatgac
    21061 accttgttct tctagataaa atagctttac aagcaggtta aaatttggta gaattgtgag
    21121 agtgtgtaat ggacgcacaa aaaacggcta atccgctgag acaagtactt aagattagta
    21181 agagaaggag aataaaaatg aatccattaa ttcaaagttt gacagaaggt caacttcgtt
    21241 ctgatatccc tgagttccgt gctggtgata ctgtacgtgt tcacgctaaa gttgttgaag
    21301 gtactcgcga acgtattcag atctttgaag gtgttgttat ctcacgtaaa ggtcaaggaa
    21361 tctcagaaat gtacacagta cgtaaaattt ctggtggtat cggtgtagag cgtacattcc
    21421 caattcacac tcctcgtgtt gataaaatcg aagttgttcg ttatggtaaa gtacgtcgtg
    21481 ctaaacttta ctacttacgc gcattgcaag gtaaagctgc acgtattaaa gaaatccgtc
    21541 gttaattttg atgatcagat tttaaaaatg cttggttgtt tgaggatagt aactatgttt
    21601 taaaactgga caaccaagac gtaaaaaatc tgcctgtggg cagttttttt actaggtccc
    21661 cttagttcaa tggatataac aactccctcc taaggagtaa ttgctggttc gattccggca
    21721 ggggacatat tcattgcatg taaatagcgg tttagagcta ttttgcccca aatccgcccc
    21781 aaatttctct gattaagttt atcgttccta tctttttgtt cttgtaattg atgtgcgtaa
    21841 acttctaaag tgatatttaa attctcgtga tctaaaactt gagagatgga aattagatag
    21901 cttgcaaatg tatgcctgag agagtgcact cgtacctcgc gaccagttat ttttcggata
    21961 gttttattga ctgcattatt tgaaagtttg tcgaataatc tgtcgttttt attttttgta
    22021 aattcatgca aaaaaaataa tgtatcattg tcaattggta tatttctgat actacttttg
    22081 ttttttgttg gcaggtatct ttggttgaaa tgataatccc aagttttatt aattgataaa
    22141 tatttgttag tgtaatcaat atcattaact gttaaaccta aacattcagc gaagcgcatg
    22201 ccagttttag cgatgaggta taacgctgca tacgattgat gttgtgattt ttctttacaa
    22261 atttttatca agcgtaagta ttcattggtt tcaagaaatt ttatctttat ttacgcccct
    22321 tattttttgc tttaacctta gtgaataaac aaaaattttt ttctatatat ccctcgtgaa
    22381 cagccatgga tacgcaggct tttacatgta tgttaaaacg ctttactgta tcttgcacat
    22441 gcgtttgact ataatgattt atgacttgtt gatatttagt ggaagtaata ttgcaaagta
    22501 atatatttcc tattatatgt ttatacgata ttcgatattc ccacccgttg tcgcgtttac
    22561 ggaaatacgc cattgatata ctccacatta gctaaagaac agggtgttca aggctacctt
    22621 gatggaaaag gctctcttag agatatttgt aaatggtatg atatctcaag tcgctctgtt
    22681 ctccaaaagt ggataaaacg gtatactagt ggtgaagact tgaaagccac tagtagagga
    22741 tatagccgta tgaaacaagg aaggcaagcc acatttgaag aacgtgtaga gattgttaac
    22801 tacaccattg cccatgggaa agactatcaa gcagctattg agaagtttgg tgtttcctac
    22861 caacaaattt attcttgggt gcgtaagctt gagaagaatg gctcacaagg tttggttgat
    22921 agacgtgtga aagggttgga gagtaggcct gatttaaccg agattgagca actttaactc
    22981 aagattaaac aattggagga acgtaatcgt ctcttagaaa tcgaggttag tttactaaaa
    23041 aagttagaag acatcaaacg aggaaacaga cggtaagact aggtaagcat ttagcggagt
    23101 tccaagtaat caagaattat tacgatgagg aatctaatgt gcctattcag gccttatgcc
    23161 aactcttgaa ggggtctcgt tcaggctatt acaagtggct caatcgtcaa aaaacagatt
    23221 ttgagacaaa aaatacaaag ctaatggcta aaatcaagga acttcataga ctctacaatg
    23281 gtatcttagg ttatcgccgt atgacaacat ttattaatcg tcaacttggg acaacttaaa
    23341 acaagaaacg gattcgttga ttgatgaaca ttctggggat tagttcagtc attcgtcgtg
    23401 ttagccatgc ttgtacaaaa gctggtgaca gattttacga agaaaatatt cttaatcgtg
    23461 aatttacagc cacagctcat aaccagaaat ggtgcacaga tgtcacctat cttcaatacg
    23521 gtctgggagc taaagcttat ctcagtgcga ttaaagacct gtataacggt tctattatcg
    23581 cttatgagat tagtcacaac aatgaaatcc acttgttatg aagaccatta aaaaggggct
    23641 agagctcaat ccaggagcca cacctatcat ccatagcgat tgaggtagtc aatatacttc
    23701 caaagaatac cgttatatca tacaacaagc tggtctgacc ttatccatgt cccggattgg
    23761 caaatgtatt gataatgcac caactgaaag tttctttggg tttttcaaga ctgagtctta
    23821 ccaccttaag aaatacaact cttatgatga gttggtcaat gatgtggcac gttatatcga
    23881 attctacaac acacaacgtt atcaatcaaa attaaacaat ctgactcctc tagaattcag
    23941 gaatcaggtt gcataactta tcttttatta tttgactgtc tacttgacag ggagccgttc
    24001 agattgctta acctttctaa atttgctaaa atagctacaa gaaaacgagc catttaatgc
    24061 ttatttctta tactgtcttg cctcacgctc tcctcgacca aaaattgagc gtgaggcttt
    24121 ttgtttcatt aaacgatgat attttcatat tcatcagttt gttttccgag agccatcaaa
    24181 gcttcgataa ggtcgataat tccaggaata aaggtaatac taaaaataat atataaaaaa
    24241 acctggccta tttttcctgc gtaaaattta tgcgctccaa tgccgcccaa aagaacgtta
    24301 ataaaacata aactactatg ttagcataag actttatttt tacaactgaa tttcatataa
    24361 atggattaga gtaagggata aaagaaatta gcatagctct tttgaaaata aaaaaattaa
    24421 tataatatg
//
LOCUS       DATRME010000024        23697 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRME010000024 DATRME010000000
VERSION     DATRME010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            66..1283
                     /locus_tag="VBC23_000957"
     CDS             66..1283
                     /locus_tag="VBC23_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6842918.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            1271..1672
                     /locus_tag="VBC23_000958"
     CDS             1271..1672
                     /locus_tag="VBC23_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842919.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            1656..1961
                     /locus_tag="VBC23_000959"
     CDS             1656..1961
                     /locus_tag="VBC23_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842920.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(2150..2222)
                     /locus_tag="VBC23_000960"
     tRNA            complement(2150..2222)
                     /locus_tag="VBC23_000960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2187..2189),aa:Lys,seq:ctt)
     gene            complement(2504..3772)
                     /locus_tag="VBC23_000961"
     CDS             complement(2504..3772)
                     /locus_tag="VBC23_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6842921.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(3765..4256)
                     /locus_tag="VBC23_000962"
     CDS             complement(3765..4256)
                     /locus_tag="VBC23_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6842922.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            4583..5053
                     /locus_tag="VBC23_000963"
     CDS             4583..5053
                     /locus_tag="VBC23_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6842923.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            5189..5647
                     /locus_tag="VBC23_000964"
     CDS             5189..5647
                     /locus_tag="VBC23_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6842924.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            5619..6089
                     /locus_tag="VBC23_000965"
     CDS             5619..6089
                     /locus_tag="VBC23_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6842925.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(6128..6844)
                     /locus_tag="VBC23_000966"
     CDS             complement(6128..6844)
                     /locus_tag="VBC23_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6842926.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYAYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            6968..7426
                     /locus_tag="VBC23_000967"
     CDS             6968..7426
                     /locus_tag="VBC23_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6842927.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            7423..8376
                     /locus_tag="VBC23_000968"
     CDS             7423..8376
                     /locus_tag="VBC23_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6842928.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            8376..9116
                     /locus_tag="VBC23_000969"
     CDS             8376..9116
                     /locus_tag="VBC23_000969"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6842929.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            9113..9699
                     /locus_tag="VBC23_000970"
                     /pseudo
     CDS             9113..9699
                     /locus_tag="VBC23_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
     gene            9900..10781
                     /locus_tag="VBC23_000971"
     CDS             9900..10781
                     /locus_tag="VBC23_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO6842930.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            10796..11641
                     /gene="pstC"
                     /locus_tag="VBC23_000972"
     CDS             10796..11641
                     /gene="pstC"
                     /locus_tag="VBC23_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6842931.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            11643..12521
                     /gene="pstA"
                     /locus_tag="VBC23_000973"
     CDS             11643..12521
                     /gene="pstA"
                     /locus_tag="VBC23_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6842932.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            12514..13263
                     /gene="pstB"
                     /locus_tag="VBC23_000974"
     CDS             12514..13263
                     /gene="pstB"
                     /locus_tag="VBC23_000974"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6842933.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            13260..13916
                     /gene="phoU"
                     /locus_tag="VBC23_000975"
     CDS             13260..13916
                     /gene="phoU"
                     /locus_tag="VBC23_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6842934.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            13916..14593
                     /locus_tag="VBC23_000976"
     CDS             13916..14593
                     /locus_tag="VBC23_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6842935.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            14586..16241
                     /locus_tag="VBC23_000977"
     CDS             14586..16241
                     /locus_tag="VBC23_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6842936.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            16396..18579
                     /locus_tag="VBC23_000978"
     CDS             16396..18579
                     /locus_tag="VBC23_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6842937.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            18618..19448
                     /locus_tag="VBC23_000979"
     CDS             18618..19448
                     /locus_tag="VBC23_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6842938.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
     gene            19664..20416
                     /locus_tag="VBC23_000980"
                     /pseudo
     CDS             19664..20416
                     /locus_tag="VBC23_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            20413..21747
                     /locus_tag="VBC23_000981"
     CDS             20413..21747
                     /locus_tag="VBC23_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6842939.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(21836..22000)
                     /locus_tag="VBC23_000982"
                     /pseudo
     CDS             complement(21836..22000)
                     /locus_tag="VBC23_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            22289..22462
                     /locus_tag="VBC23_000983"
     CDS             22289..22462
                     /locus_tag="VBC23_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6842940.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            22531..23379
                     /locus_tag="VBC23_000984"
     CDS             22531..23379
                     /locus_tag="VBC23_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842941.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            23385..>23697
                     /locus_tag="VBC23_000985"
     CDS             23385..>23697
                     /locus_tag="VBC23_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842942.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TF"
BASE COUNT         7540 a         3802 c         4908 g         7447 t
ORIGIN      
        1 ataatgattt agaaaccaat aaaaaatgaa aaaaatatat tgatgaagaa aaattacaaa
       61 tcgcaatgtc agaatataaa acaatccatc ccaacgccag attaaaggat caatatgata
      121 acacaattgg agttgtttcg gaggtttacg acaatacttc tcattctgga gaacagattt
      181 atgctgtggt tgataatcgt catattccaa aagaagatgt aaaagaggtg acggtacttt
      241 ttcgtggatc aacatctcct caggaattta ttaataatcc tgcagatgtg actttggatt
      301 ggttagaaaa tgacattcct atggcaagta atatttgggt aatgaaagat tttgggaatc
      361 ctcataattt cagtgctgta tcaccacagt tgaaagcttc atcagaacat ttaaaagaga
      421 ttatgaaaaa atatccaaat gcaaaaataa acattggtgg acattcatta ggaggtatgg
      481 atgctcaata tgctgttgta gatattacag ataagaaaga cttgaaacgg attaactctg
      541 tacatgttta taatgctcca gatatatatc ctacacttac taaaggacag aaaaagacgg
      601 cagattcact gaaatctaag attgttgttt atgttgatcc taatgatttt attggcatgg
      661 tggggcgtga aggaaaaaaa ggaagtgaag actctgtagg aacagtctat tatacagaaa
      721 gtccagatat caactggata gatcaacaca tgacctatgg ctatcgtatg gaaaatggtc
      781 agattaaagt tattgaaacg aatttaccac cggaagttag gactactaga aaagctatgg
      841 gatcttttta taaggacaag aaaaaattta aaaaatctgg taagggtcta agttcgcatg
      901 aaaaaatctt cttggatgct gaacaagctg ctgttattgc aagtggttta gtctctactg
      961 ctgaaactgc tttagaggat atcaaaacat ctgcgaaaac agctgttgga gaagcagaag
     1021 aattgtggaa tacgactaaa tcgattcctt ttggtattac ggagcttaca gaagcagagt
     1081 tggcagaagc ttatgcagaa ggtggtgtca cttatgatag catagtaact aaaactgata
     1141 ctcattttga tgagaaagtc aaaaatgcag gagatttagt aacaacatat acgactttga
     1201 aaagtgatat tcaatcaggt gttgaaacca tgttatctaa ggatagtgaa ttagctggag
     1261 attttaaagc atgggaaaat tagacgctaa agatgataaa ttaagtaaca gtaaaaagga
     1321 tttagaaaag ttggaagatg attaccatca taaaaccata gctatttcag ataaattctt
     1381 tgaactggaa gataaacgat gtgagtttga gacgatgctt caggaaactt acgaagcaac
     1441 aagttatcat cttcgtcagg atgagatgat taatgaagag tcctttatga ccatgaatca
     1501 cattatagaa gcttttcaaa gtgattttga tacagaatat actaaagaga aaagaagatt
     1561 gactgcctta gaagaagaaa ctaatcaaaa gtactctaaa aagagacaat tgcttgaaga
     1621 aaaaatagat catttaatgt cagaaaggag agactatggg aatccttggt gattatgttt
     1681 tatcagaagc ctctgattat atcagtggaa aaaaagatat caaggcttta aaaaagaaac
     1741 ttgataaaat gttagtgaca ggtgtttatg ctccaagaat caagtctaaa cgtagaagta
     1801 ttgtctctac ctatgatgca gagataacga ctactgcaac aaacgcaaaa gctagtataa
     1861 ctgccattgc aggtcaaata gacactgcaa taaagggaca gtttagaact aaagttgaaa
     1921 ctgtattgga taataacagt acaaagtatg atgaaattta aaagacatgg gctaaataat
     1981 ttagctcttt ttttatactc aaaaataaga gaaatacaaa acaaaataca tatttatgca
     2041 agtaaagaga aataaaaaaa tcaccatttt atctgtcaaa aaaaattggt gggcaagtgt
     2101 aaaaagagcg tcaaaaaagc ctataggtat aggctttgtt ttataagact gccccctaca
     2161 gggctcgaac ctgtgaccca tagattaaga gtctactgct ctaccaactg agctaaggag
     2221 gctaggaagc tgtattggca ccgatgatcc actttttgta ttgatcccgc acaaaataag
     2281 caggtgggta actcgcacct ctttaagtgg cttccgcgtg ttaacggctt gcgcactaga
     2341 gatgacttcg tttcccgaaa tacaaagaaa tagtcggtca acacttagat gtggattcgt
     2401 atgccacagc aatgtttcta tgtctttatg ataccacttt ttataaaatt ttcaagcaaa
     2461 attatcgatt tttgaaatcg attgcgattt ttaaaactgg caatcagtct gagagcttat
     2521 caatgcgctc tttatttgaa ccaagtgcac gttcgtattt accagtttca ttggcttcaa
     2581 aataattatg tcctactagt ttatctggta agtattgttg tttaacccat ttttcaggat
     2641 aggcgtgagg gtacttgtaa tcccttgcat tgcccaaggt tttactaccg ctgtaatgtc
     2701 catctcttag gtgacgtgga ataggtaaat ttcctgaacg acgtaaatca gctaaggctg
     2761 catccatagc caaataggct gaatttgatt taggtgatag agctaaatcc acaacaatgt
     2821 tagcaataag aatacgagct tctggaaaac caatgcgttg ggcagcttcc aatgcagtca
     2881 cagtatgtat ttgtgcttcg gggttagcta agccgatatc ttcataagca ataatggtca
     2941 aacgtcttgc taaactaggt aaatctccag cttctactaa acgagcggca tagtgcaggc
     3001 tggcattgac atcagaacct ctaattgatt tttgtaaggc tgataggaca tcataatgcc
     3061 catctccgtt tttatccatt gtaatataac tacgttgcag actattttcc attgtctcta
     3121 gactaatatg acggctacca tcttcgttag gagaagtaga catgactgct agatccaagg
     3181 aattataagc agatcgtagg tctccatttg tggcggtaac aatgaaatct agcgccccat
     3241 catcaattgt cactaaaaaa ggaaatcccc gttctttatc ggaaattgct agttgaatgg
     3301 cttttttaat atcctcgtta gaaagaggtt ctagctcaaa aatttgtacc ctacttctaa
     3361 tggcaggagt tactgaaaag aaaggatttt cagttgttgc tccaatcata ataatattgc
     3421 cattctcaag taaaggtaaa agaaaatctt gttttgtctt gtcaagtcta tgaatctcgt
     3481 caagtaataa aactaagcca ccagaaaatt tagcttcttc tgcaatttct tgaagacgtt
     3541 ttttactatc aacagtggca ttaaaagtac gaaaggcata tttagtagta ccagcaatag
     3601 cagaagcaat agaagtcttc ccaatacctg gtggtccata taggatcatg gaagataaca
     3661 tattagctgc aaccattcga tcaataattt tgccattacc aacgagatgt tgctgcccaa
     3721 tgacttcatt aatattgcga ggacgcatac gtagagcaag attgtcagcc atgattattc
     3781 tccaaatctt ttgcgaaaat taaagcatca acataatgat tttcaatgaa aaaggcttta
     3841 gtgattcgag cctcttcggt aaaaccgtat ttttgataga gggaaatggc tctactattg
     3901 ttgcccatga cgtgcatggc aatcttgatg taaccttgag ctttagcctc agttagcaga
     3961 gctttcaaga gagccttacc tagcccctga ccttggtagg gttcagcgat gagaatgccg
     4021 aaagtggcaa catgcttgcc agctgggaaa ggatagtagg gaccaaagtc caagacgcca
     4081 gcgatcttat catcgatctc agcgacaaag aaggacatgc catttgccag attcttctcc
     4141 aagtcactct cagtccactt gtggtcatag acaggagagg taagagcagt ccagccagta
     4201 ttttgtaagg tcagaactgc ctcacggtcg cagttctgca taggtctgat aatcataatt
     4261 gatgatacaa agggacggtt cggtttacga aaattttcgt aaagaaatag ggaacgacga
     4321 agtgtcgtta gacactagga agttcatctt ttcttttaga aaattagtcc cgtgttcaaa
     4381 tcctttctat tttttatata gagtttttct ctcagctgac tcttttaaca tagtatgaag
     4441 tcagtcaaaa acgactagtc tttgagacta ttgtaacata aaaataagta ggaactacct
     4501 tttgattaac ctgagtctgt aacgtttttc ttctatattc atgttataat aagaaagaat
     4561 aaatagaaaa taaggacata agatggctaa atttggattt ttatcggtac tagaagaaga
     4621 attggataag catttacagt atgactttgc catggattgg gataagaaaa atcatactgt
     4681 agaagtcact tttatattag aagcacaaaa cagctctgct attgaaaccg tggacgacca
     4741 gggggagatg agtagcgagg acatcgtttt tgaggactat gtactctttt acaaccctgt
     4801 caagtctcgc tttgatgctg aggactatct ggtgactatc ccctatgagc ctaagaaggg
     4861 cttgtcgcgt gagtttctgg cttattttgc ggagactctc aacgaggttg ccactgaagg
     4921 gttgagcgac ctcatggact tcctcaccga tgacagtatc gaagagttcg gtctttcttg
     4981 ggatacggat gcttttgaaa atggcagagc agaactgaaa gagacagagt tttaccctta
     5041 tccgagatat tagacttgat ggatttgcta aaatgtggat atacattgga tgatatagaa
     5101 acagccagac cagaggatat ggaacgttat tatccccctg aacaaatcag aaaatatgga
     5161 gctttaggaa tagaattgag gttattgcat ggatattttt gatgagttat ttgatttctc
     5221 aggtgcaaag atcgctcttt tctgcgaggg aaagattttg accagcctaa gagatgattt
     5281 tccagatttg ccctatgcag gcttttggga cttaccaggc gatggtcgtg agggcgatga
     5341 aacacctttg gaatgtcttt ttcgagaagt tagtgaagaa ttaaatctga ccttaactcg
     5401 aaaccatatt gattgggtca agacttatag gggtatgctg aaccctgata aattatcggt
     5461 ttttatggtt ggacatatca gtcagaaaga gtacgatagc attgttctag gcgatgaagg
     5521 acaagactac aagctcatgt ccattgacga atttttaagc cataaaaagg tcattcctca
     5581 gttgcaggag aggttgagag attatttgga ggtggaagat gattacttta caaaaggcgg
     5641 aagctagtga tttagagaag attattgcca ttcagagagc tagctttaag gcagtctatg
     5701 agaaatacca cgatcaatac gatccctatg tggaagaagt tgagcagatt cgatggaaac
     5761 tagtggagcg accagattgt ttctatcatt ttgtccttgt tgatgagaca attgttggtt
     5821 ttcttagatt ggttatcaag gacgaggaga agcgtgcttg gcttggtacg gcggctattc
     5881 ttccccaata tcaaggacag ggctacggct cagcagcgat ggctttatta gagaaaacct
     5941 atcctaagct tacaaagtgg gatttatgta cgattgcgca agaaaaacta atggtcagtt
     6001 tttatgaaaa atgtggttat cattcaaccc ataccgaacc tgagcaagaa ggaatggata
     6061 tggtctatat gacaaagaag atagaataaa aaaagaaaac tagatgattg tctagttttc
     6121 tgatagttta ttggcaatag taagcaatag cttggctgac aaattcactt gttcctgctc
     6181 caccagcttt gtcaattgat tgactaaacc ggtcatctga ttgatacatt tgtcctagtc
     6241 cagcaaggat ttcattggta caggtgtaaa aatgctgaga gatatagtct tgtagccgtt
     6301 tcacacacat ttgaacagat tcatcagata cattttgtgt ctttaattgc ccaaattgta
     6361 ccattatttt tgccatttct tggctaattt gtgcaaagtc atctgaagca tgtttttgag
     6421 caaattcttg gtaggctgct gtttgtcccc atttttcttt tgcttcagct tgtaattgtt
     6481 ctaattctgt tttattataa acatcgaaat tcatataagc atatcctttt tcttgtaatc
     6541 gtttcgcatg ggtgataacc tgctcaagat gcgatctttt ccattcaagc aatttgattt
     6601 gttgggctaa taagtcttgc ctttcctctt ttgcttgctc aagcaggtat ttgatatctt
     6661 tgagagggaa ttccaactca cgaaatagca aaatttcttg caggcgaatc aatgcttcat
     6721 cgtcataaag tcgatagcct gcttcggata gggcggtagg tgggaataaa ccgattttat
     6781 catagtgatg taaggtgcgc acacttacac cgcttaagat actgacttct ttaactgttt
     6841 tcatggtaat aacctcctct cacttacata gtataaagga taacctaagg tcagggtcaa
     6901 gcaaaaaata aattattttg aaaagtatgg ttaatcatat tgatttgaag ataaaggagg
     6961 gatagttata gaaatagttg agctactaag ggaatggtat tcttcctttg aagttaaaga
     7021 agaaaagtgg aatgaaaaat atgaaggtat cctcataaga attgaaggcg atggaaacta
     7081 taaaaggtgt cgtttagctc ataagacagc tagcaagatg gggtatttta cagtattttg
     7141 gcagaaagat ggaaatggta aaaatattcc tttttatgaa caagatgaag tggaagattt
     7201 gattattgtc attaaagatg gtaggaggaa aggtttgttc atcattccca aagaggttgc
     7261 agtgagtaag ggtattcttt catctgctaa ctctcaagga aagatggcta tgcgatttta
     7321 ctctccttgg tgtagcgatt taaatagaac agctttggtg acacagcgat ggcagttgaa
     7381 ctattttatt gatttaagta aaaataatga aggagttact acatgaacac atggaatgaa
     7441 ctaacggttc acgtgaatcg tgaggcagag gaagctgtct cgaatttatt gattgaaact
     7501 ggcagtcagg gggttgccat tagcgactcg gctgattatc ttggacagga ggatcgtttc
     7561 ggcgagcttt atcctgaggt ggagcaatct gatatgatcg ctattactgc ctattatcct
     7621 gatacgcttg atattgaggc ggttaaggct gatctggctg atcgtttggc taattttgaa
     7681 ggctttggtc ttgcgacggg tagcgtcaac cttgacagtc aggaattggt tgaagaggac
     7741 tgggctgata actggaagaa gtactatgag ccagctcgca ttacccatga tttgaccatc
     7801 gtgccgtcat ggacggacta cgaggctaag gctggtgaga agattatcaa gctggatcct
     7861 ggtatggctt ttgggactgg gactcaccca acgactaaga tgagtctttt tgccttggag
     7921 caagtgcttc gtggtggtga gacggttatc gatgtgggga ctggttctgg cgttttgtct
     7981 attgctagct ctctcttggg tgccaaggac atctatgctt atgatttgga tgatgttgcg
     8041 gtgcgcgtgg ctcaggaaaa tatcgatatg aaccctggta ctgataatat tcatgtggct
     8101 gctggtgacc tgctcaaagg tgtccagcaa gaggcagatg tcattattgc caatattttg
     8161 gcggatattt tgatccatct gacagacgat gcctaccgtt tggtcaagga tgagggttat
     8221 ctcatcatgt ctggtatcat ttctgaaaaa tgggacatgg tgcgtgagag tgctgaaaaa
     8281 gctggtttct tccttgagac tcacatggtt caaggtgagt ggaatgcctg tgttttcaaa
     8341 aagacagatg atatttcagg agtgattggc ggctaatgca acagtatttt gtaaatggca
     8401 aagcagatga ctttgtgacc atcgaggaca aggataccat caagcacatg ttcaatgtca
     8461 tgcgattgac agaagatgac caagtggtct tggtttttga tgatgccatc aagcgtctgg
     8521 caaaggtggt ggacagctct gcccatcgct tccaaattct agaagagcta gacagcaatg
     8581 ttgaaatgcc tgtccaagtg accatcgcct caggttttcc caagggagac aagttggatt
     8641 ttgtgacgca aaaggcaaca gaattgggtg ctgcggctat ctggggattc ccagctgact
     8701 ggtcagtcgt caagtgggac ggcaagaaac tagctaaaaa agaagataag ctggcaaaaa
     8761 ttgctctggg cgcagcggag cagagcaagc gtaatcgtct tcctcaggtt cgtttatttg
     8821 agaagaaggc tgattttcaa gcggagcttg caggctttga taagattttt atcgcctatg
     8881 aagagtctgc caaggagggt gaaatgtcag cgttagccca gaatttgcag actgtaaaag
     8941 caggtgacaa gctcctcttc atctttggac cagaaggggg catttctcca aaagaaatcg
     9001 cagcttttga agaggttgga gctatcaagg tgggcctggg tccacgcatc atgcgcacag
     9061 aaacagcacc gctctatgct ctaagtgtta ttagttactc agcggagtta ttatgattat
     9121 tgctattcaa ggtgatatgg ctgtagggaa aaccacagct ttgaaaatca ttgagaaagt
     9181 ttacccgaaa atcgctatta gttttgagga tatttcacca gtagttgata agattaataa
     9241 acatcagctt gataacaata aattagacga ttattgtgaa attcaacaac tatttattca
     9301 gcatgagcga gatagatacg aacttgttag agataatgaa atagtgttaa tggattatgg
     9361 agctgaggaa attgctttct atacacaata ctttccaaag gtggcgggtt atagttgact
     9421 ttaccttcta atttgttact agaggtaaat aggctaaagg aatgttttgc tgattatatt
     9481 atttttctag atgcagatga gacggtccta taccaacgtc aaaaaaatga ttttaataga
     9541 cggcgtcatt ttttcaaatg gtatactcat cacctgttgc ctgctaaaaa acattattta
     9601 gacacatttt ccaatgttat tcatattaat acaaatcatt taaatgctga agaagtggct
     9661 gaaaaaatca ttgacatcat tcgtaagtta actctataat gggatagtta tacttcaatt
     9721 atgtggtata acaactgttt caagccgact tattggctga attaccataa tagaaaaaag
     9781 gagtttggat aattttccaa ctctttttaa ttttacattg attttacaaa aatcctcaaa
     9841 gagatttgat attccttaac tataataaaa tcaaatcaaa aaatatttag gagttcatca
     9901 tgaaaaaaca caaaatgcta tctcttttgg cggtatctgg tcttatggga attggcatcc
     9961 tagcaggatg ctcaaatgat tcatcatctt catctaaagg gacaattaac attgtttcta
    10021 gagaggaagg gtctggaact cgtggagctt tcatagagtt atttgggatc gagtcaaaaa
    10081 ataaaaaggg cgaaaaagta gaccatactt ctgatgcagc aactgttacc aattcaactt
    10141 cagttatgct aaccactgtc tctaaagacc cgtcagctat tggttattct tcactaggtt
    10201 cattaaatag tagcgttaaa gtgcttaaaa ttgatggcaa aaatgcgact gtaaaagata
    10261 ttaaatcagg aagctataag atttctcgtc ctttcaatat tgtaacaaaa gaaggtaagg
    10321 aaaaagaagc aacaaaagat tttattgatt atatcttaag caaagatggg caagcagtcg
    10381 ttgagaaaaa tggctatatt ccacttgaca atgctaaagc atatcaagca aaagtgtcat
    10441 ctggtaaagt tgttatagca ggttcaagtt cagtaacgcc ggttatggaa aagattaaag
    10501 aagcctatca taaagtaaat acaaaagtag atgttgaaat tcaacaaagt gattcttcaa
    10561 ctgggattac aagcgcaatt gatggatcag cagatatagg tatggcatca cgtgaattgg
    10621 ataagacaga gtcatccaaa ggggtcaaag ctactgttat tgcaacagat ggtattgcag
    10681 tagttgttaa taagaaaaat aaagttaatg atctatcaac aaaacaagta aaagacattt
    10741 ttactggaaa gacaacgtct tggtctgact tatcaaagta aggtaagagt aaacaatgaa
    10801 acaaacaaaa gaaggtctta tgcaagctgt gtttttcatc acggcttgcg tcagtgttat
    10861 atcagctatt ttaatttgcc tctttctctt ctctagtggt cttccagcta ttacaaaaat
    10921 cggctgggga aattttatat ttggtaaagt ctggcatcct agtaataata tttttggcat
    10981 ctttccaatg attgtaggtt ccttatatgt tacagcggga gctttacttt taggaggccc
    11041 gataggtatc cttactgctg tttttatggc ttatttctgc ccagaaaata tttataggcc
    11101 tttaaagtca gctatcaatt taatggcggg aattccttca gttgtttatg gtttctttgg
    11161 actcgttgtc attgtcccta tgataaggca atatattggt ggctttggta tgggagtctt
    11221 agcagcttca attttgctag gaattatgat tttaccaaca attgttagca tttcagaatc
    11281 ttctttaaga gctgttccag agagctatta tgaaggcggt attgcccttg gtgcctctca
    11341 tgaacgaagt gtcttctttg ctgttttacc agctgctaaa cgtggtattt tagcatcggt
    11401 tgttttggga attggacgtg ctattggtga aacaatggct gtcatcatgg tagcgggtaa
    11461 tcaagctgtc ttgcctcaat cgctgacatc aggcgttaga actctaacca ctaatattgt
    11521 catggaaatg ggatattctt ctggtttgca tcgtcaagcc ttaattggta cagctgtggt
    11581 actctttatc tttatattaa tgattaatat cagtttttca gcattacaga aagaggaata
    11641 agatggaaaa tattaaccaa agaacgtcaa aacaatattt agcatcttta aaaaaagatc
    11701 ccctttcctt ctttcttttt gctattgtct atttgggagc aattctaagt tttgctacaa
    11761 ttgcatttgt tgttatttat attttagtta aggggcttcc tcatgtgaat actggcttgt
    11821 ttgcatggac ttataatact cagaacgtct cactattacc agctttcatt aacactatct
    11881 ttatcatcgc cttaacttta ttatttgcag ttccccttgg tattggtgga tcaatttatc
    11941 taacggaata tgctagacgt gataatcctt atctcaaaat tatccgtgtt gcaaccgaga
    12001 cattggcagg gataccttca attatttatg gtttatttgg tgcattattt tttgtaaaat
    12061 acacacactt aggcttatct ttgatatcgg gttctctgac tttgagcatc atgattttac
    12121 ctttgattat gaggacaact gaggaagctt tactatctgt tccggattct tatcgagaag
    12181 gagcctttgc gcttggtgct ggtaaattga gaaccatatt taaaatcgta ttacctagtg
    12241 ctatgtctgg tatttttgct ggtattattt tagcgatagg acgtatcatt ggtgagtcag
    12301 cagcacttat tttcacagct ggaacagttg caaaagtagt acattcggta tttagttcat
    12361 ctcgtacttt agcagtacat atgtatgcta tttcgggtga gggcttatat gttgaccaaa
    12421 cttatgcgac tgcggtcatc ttattgcttt tagttattat tgtcaatttt gtatctggac
    12481 tagtagccaa acgattagga agtgagtcga acagtgagta aattagtaat taataattta
    12541 gatttgtatt atggagaatt tcacgctctg aaggatgtta atttagacat tgaagaaaaa
    12601 gaaatcacag cctttattgg accttctggg tgcggaaagt ctactctctt gaaaagtatt
    12661 aaccgtatga atgatttggt caaaaattgc aaaatcacag gggatattac tttagaaggc
    12721 gaggatgtct atagacaatt ggatattaac caattgcgta aaaaggttgg aatggtgttt
    12781 caaaaaccta atccgtttcc aatgagcatt tatgataatg ttgcttttgg tccaagaacg
    12841 cacggtattc attccaaagc agagttagat gatatcgttg aacgttccct taaacaggcg
    12901 gctctatggg atgaggttaa agatcgtctt cacaaatctg ctctgggtat gtctggcgga
    12961 cagcagcaac gtttgtgtat tgcgcgtgct cttgctattg agcctgatgt tttacttatg
    13021 gatgaaccca caagtgcctt ggatcctatt tcaacagcaa aaattgaaga attggttatc
    13081 caactgaaga agaactacac tattgttata gtgacacata atatgcaaca agctgttcgt
    13141 atttctgata aaacagcatt ctttctgatg ggagaagtgg ttgaatataa caaaacgagc
    13201 caactctttt cattgccaca agatgagcgt acagaaaact acattacagg gagattcgga
    13261 tgattcgttc acgatttgca agtcaactta atgaccttaa taaagaaatt atttttatgg
    13321 gggcattgtg tgaggacatc attgggaagt ccttaggtgc tctaacaaat agtaatgatg
    13381 tttaccttga cgatatttct gaaacttatc acaagattga acaaatggaa cgggatattg
    13441 aggagagatg tttaaaactt ttacttcgtc agcaaccagt tgccaaggat ttacgccgta
    13501 tttcttctgc attaaaaatg gtttatgata tgaaacgtat tggtgctcaa gcttatgaaa
    13561 ttgcggaaat tgttagttta ggtcatatta tccaaggtgc aggttctgaa agggatcaac
    13621 ttaacagcat gtctaataat gtcattagta tgctgactaa gagtatagat gcttttattt
    13681 atgacaatga agaacaagcg catcaagtaa ttgaacaaga taggacagtt aaccaggaat
    13741 ttgatacaat aaagaaacag ttagtgttat atttttcggt acaggatgtt gatggtgaat
    13801 atcccataga tgttttaatg atcgccaagt acctagagcg tattggtgat catacagtaa
    13861 atattgctaa atgggtgctt ttctcaataa caggtaacct agatggagaa gcgtaatgat
    13921 atattgtgtt gaagatgatg ctgatattcg cgaaatgatg ctctatactt tgcaaatggc
    13981 aggttttaca gctcaaggat tctcgagttc agagcttttt tgggaagctg ttcaggagaa
    14041 agttccagac ttgattttac ttgatattat gttgccggga gatgatggtt tgactatttt
    14101 agagcgttta aggagaaaac atcaaacgga aatgatacca gtaatcatga caactgctaa
    14161 aggtagtgag tatgataaag ttaaagggtt agatcttgga gcagatgatt atctcgtcaa
    14221 accttttggg atgatggaaa tgatttcacg aataaaagca gtcttgagac gtagccgcca
    14281 agtagattca aaagctcata ttatcattgg aaatttagag attgacccga ctaattattg
    14341 ggtcaaaagg ggaacggaaa aaatccactt aaccctaaag gaatttgagt tattagtatt
    14401 attcttccgt aatcccaata gagtctttac aaggcaagaa cttctggata aggtttgggg
    14461 agaacaattt ttaggagaaa ctagaactgt ggatgttcac attgggacac tgcgaacaaa
    14521 acttggtgag gatggctatt tgatcgctac agtaagggga gttggatacc gtttggagga
    14581 aagacatgac taaaaaaatt ttccgtacaa ctttatccgc tagtttggga attgttttag
    14641 ttacaatttt gatgattatg ggatttctct ataattactt caatcgcatt cagcgagaac
    14701 agttgagaac acaaacggca ctggcgtcac aaggaatttc ctttgaaggt aaagattatt
    14761 ttgaaaatct taaaacttca aatgtgcgta ttacttgggt agataataag ggacaagtgc
    14821 tttatgatac ccaatcagat gcaaaacata tgaaaaatca cgccaatcgt caagaaatta
    14881 aagaagctat caagagtggt tatggtgaga gcacgaggtg gtcagcgaca cttactgaga
    14941 agtctatcta tgctgcgcaa cgtttaaata atggaacgat agttcgttta tcagttgcgc
    15001 aacaaactat tttttatctt ctccttggta tgatgagtcc cttagcaatt atcatcttac
    15061 ttgctattat tttatctgta ttaattgcac gatatatagc aaaaaaagta tcagagcctt
    15121 taaacaatat tgacttagat catcctttat caaatgatag ttacgaggag ataacaccat
    15181 tattaaggcg tcttgatagc catcaggcta agattcaaca tcaaaaactg ttattacaaa
    15241 agcgtcaaaa agaatttgat accattattt ctaaaattaa ggaaggaatg attcttttag
    15301 atgatcaagc aagaatcgtt agtatcaacg cagaagcttt aaagttattc caaattaatg
    15361 atgattggca tgggcgcttt atgatggaag ttagccgaga tttaactctc aaagacttaa
    15421 ttgatcaagg actaaaaggg aaaaagaaag aggctaatat tgacattgaa aataatcatt
    15481 accgcgtctt ggtaagacca actacagata acaaccgagt aacaggattg gttgttctac
    15541 tgtttgatgt gactgaccaa ctccaaatgg aacaacttca acgtgaattt actgccaatg
    15601 tctctcatga attgaaaacc ccgcttcatg ttatttctgg ttactcagaa ctgttagcta
    15661 atcaaatggt tccaaacgaa gaagtcccac aatttgcagc taaaattcat aaagaatctg
    15721 aacgattagt taaattggtt gaggatatta tcaacctttc tcaccttgat gaacaagaaa
    15781 aattaccgca agagacagta aatctgtatg acttaacaca aaaagtttta gaaggtttac
    15841 aagctaaagc agataagaag cacatacaaa tcaattttaa tggagaagaa gctattttgc
    15901 gagggaatcc agttttattg aattccttgg tgtacaactt gtgtgacaat gctatcacct
    15961 ataatcatga gaaaggtcaa gtaaatgtga ctttgaaaaa ttcacctgat acaattacct
    16021 tagaagttag tgatacggga ctaggcattg cagagaagga caaaaaacgt attttcgaac
    16081 gtttctatcg cgtcgataaa agtcgctcaa aaatagttgg agggactggt ttaggtttgt
    16141 ctattgtaaa gagtgcttta gattttcaca atggtagtat aaaagtggac agccatttgg
    16201 ggcagggaac aacaatgacc gttttacttc acaaacaata aaaactcaca aataaaagtc
    16261 tagatgatat tatctagact tttttagtaa ttcagaaaaa atatataagt aagggtttac
    16321 aaaaaacaaa taagtgctat aataaaacca taacgcaaac gtttgcacta ttaagtgcat
    16381 aaaaggagat tgaagatgaa aaataatgtc aaacagctct ttagttttga attttggcag
    16441 aagtttggga aggctttgat ggttgttatt gctgttatgc cagcagcagg attgatggtt
    16501 agtattggta actccattag gctattggat ccatcaaatg ttttattagg gcgcatagct
    16561 aatgttattg ctcaaattgg ttggggagtt attggtaact tacacatctt atttgcattg
    16621 gctattggtg gtagctgggc taaagaacgt gccggtggtg cttttgcggc tggcttatcc
    16681 tttattctta ttaaccttat tacagggaac ttttttggtg tgaaaacaga tatgttagca
    16741 aacagtaaag caactgttca aacggtattt ggtgcgacca tcagagtttc agattacttt
    16801 gttaacgttc ttggacaacc tgctcttaat atgggtgtat ttgtcggtat tatttcaggt
    16861 ttcgttggtg caactgcctt taataaatat tataactatc gtaaattacc agatgcttta
    16921 acttttttca atggaaaacg ttttgttcca tttgttgtta tttatcgttc agtcattgtt
    16981 gcattaattt tatcagtatt ttggccggtt gttcaatcag gtatcaatgg ctttggtaag
    17041 tggattgcaa gttcacaaga cagtgctcca atattagcac catttgtcta cggtacgtta
    17101 gagcgtttat tgctcccatt tggtttacac catatgttaa ctattccaat gaactacacg
    17161 caactaggtg gcacatacac tgtccttaca ggtgcaacta aaggagcaca ggtgttggga
    17221 caagatccac tgtggttagc atgggttggt gatttaatca acttaaaagg ctcaaattca
    17281 agtcaatacc atcacttatt gacaagtgtc actccagcac gttttaaagt ggggcaaatg
    17341 attggtgctt caggtatttt gatgggatta tcttatgcta tgtatcgtaa cgttgataaa
    17401 gataaaaaat tgaaatacaa gtcaatgttt atttcagcag cagcggctac tttcttgaca
    17461 ggtgttactg agccaattga gtacatgttc atgtttgcag caatgccact ctaccttgta
    17521 tatgctgtcg ttcaaggatg tgcgtttgct atggcagata ttgttaacct ccgcgttcat
    17581 tcatttggta atatagaatt tttaacacgt gttcccatgg gaataaaagc gggccttggt
    17641 ggtgatattt tcaactttgt ttgggtgaca cttctttttg cagtattgat gtactttatt
    17701 gcaaacttta tgattaaaaa attcaattta gcaacagcag gacgtaatgg taattatgat
    17761 aacgaagagg ttgataatgc tccttcaaca gcttctggtt ctgctgatgc aaattctcaa
    17821 gttgtacaag ttattaattt acttggtggt cgtgataaca ttgaagatgt agatgcatgt
    17881 atgactcgtc ttcgtgtcac agttaaagat gggaatagcg tgggttctga agcagcttgg
    17941 aaaaaagctg gtgcaatggg attggtgtta aaaggtaatg gcgttcaagc tatttacgga
    18001 cctaaagcag atgtccttaa atcagacatt caagatttgc tagattcagg tacagtgatt
    18061 ccaatagttg atttagaaac aggccagcct gtagcagcaa cccaagttac aacttataaa
    18121 ggaataacag aagaaattgt ttcggtggct aatggtcaag tagaagcatt ggatgttgtt
    18181 aaagaccctg tcttttctca aaaaatgatg ggtgatggct ttgctgtgga accaactgat
    18241 ggcaatatct atgttccagt ttcaggtaca gttactagtg tctttccaac aaaacatgct
    18301 tttggtttat taacagaaag tggcctagaa gttttagtac atatcggtct agatacagta
    18361 gctcttgatg gtcaaccatt tgaggttaaa atttctagtg gtcaaaaagt ggtagcaggc
    18421 gatcttgctg tagtagcaga tttggaagct atcaaagcag ctggtaaaga gacaagtgtg
    18481 attatcgtct ttacaaatgt ttcagatata aaaactgtta aacttgaaaa atcaggtcca
    18541 caaatagcta aaacagttgt ggcaaaagtc gaactgtaac aaacgatagg agctgggcaa
    18601 gtctcagctt ctttctgatg aaaggatact ctatggcaaa atgcttaacg ttaaatactc
    18661 attcatggat ggaagtaaat gctctaaaga aattatttga tttagcagag catatcttcc
    18721 gtgaaaaata tgatatcatc tgcctacaag aggtgaacca gagcatatct tcccctcttg
    18781 ctacgagtag tccaaattat catccgattg aaggtacacc agctcttcat caagataact
    18841 ttgccttgca attagtgcat tacctaaatc ttcaaggtct acactatcat tggacatggg
    18901 cttataatca tattggttat agtaagtacc atgaaggggt agcgatactt tctctgaaac
    18961 cattaaagcc agatgatatt ttagtttcag ctgtagacga tgagactgat tatcatactc
    19021 gtcgtgctct ggtagctgag accactctga atgacaaagt cgttacagta gttagcctcc
    19081 atttatcatg gtttgagaaa ggatttgcgg aggagtggaa acgactagaa actaccctac
    19141 ttgaggtaga aactccactt ttactcatgg gtgactttaa taatcctact ggaaatcagg
    19201 gttatgaact tgttcttaat agtccattag cattaaagga tagccaccaa atagccaatc
    19261 atgtatttgg agaccatact attatggctg atattgatgg ttgggaaggg aataaaaagg
    19321 ctcttaaagt tgaccatatt tttactagcg aagacctttc aatcagttca agtcaagtag
    19381 tttttgaagg tggtgaagca cctgtcgtta gtgatcatta cggcttggaa attacgttaa
    19441 atctttagat taatctattt aatatattaa aagcatccta aatttgtcta taaaacaaaa
    19501 cttagggtgc tttttgaaaa atatgtcaca ttatttaact tcttacgaag tttggaatgc
    19561 atgagaaaat atgataaaat gataataact attataggtg attttaataa ataatttaat
    19621 caattggttt atctattaga acaattaata cgaggagacg agagtgaata tttttatttt
    19681 agaggataat tttattcaac aggcacgttt agagagaata attgaggaag ttaaaattag
    19741 gagaagtata cacagtaaaa tggtaaatac ctttgaaaaa ccagttcaat tattggaatc
    19801 aatctgtgaa aaaggtatgc ataatttata tttcttagac attgaaataa aaaatgatga
    19861 gcagaggggc ttagaggtcg caaaacaaat tagacaaatt gatccttatg ctcagattgt
    19921 ttttgttaca actcattcgg aattaatgcc tttaaccttt cgttatcagg tatcagcttt
    19981 agactatatc gataaaggac tttcacagga agcatttagc agaaggattg aagacgtttt
    20041 actatatgct aataatgctt gcagtaaaaa cttactagaa gattcttttt attttaaatc
    20101 acgctatagt caagtacaag taccttttaa cgatatcctt tatatagaaa cgtcattaag
    20161 gtcacataga gttgtccttt atactgcaaa agacagaatg gaattcacag ccactttggg
    20221 ggatatttta aaacaagaac ctaggctctt tcaatgtcat cgctcttttt tagtaaatcc
    20281 cttgaatatt tttaaagtgg ataggtcaaa taaactaatc tattaaaaaa atggttcgaa
    20341 ttgtttgatt tctcgtaata aaataaaaga tattatggta gtaatagagg aaaatcaaag
    20401 aggtagagga atatgaattc tactctatcc atcttagaga tagctttaac gataatatta
    20461 acaatatttc tcttttctaa aatcagtgga cgtcaattga ctttaataga gattggaatt
    20521 tgtattgctc tgagattagg acttgtaatt ttatttatag cagctaatac tattattaat
    20581 ctttcatttc taaagtactg gcttctcccc atctatatgt taagtctggc tttcttgttg
    20641 ctaagaccta tatctaaaac tttagtagtc ttttatggct tgtttccaat tgttctcaga
    20701 aatctttgtt cacgttcttt agcttttttt gtctttcctt tgtttaatat tgattttcat
    20761 acaaccaacc aggggttttg gggatatttt gttgatgtct tgtctactct ttgtgttctc
    20821 ctatttttaa gatggatgca atacgacttt aaaactttgc gaagtagtga agtttcgccc
    20881 atagataaga gaattctcca atttacgaat ggttgtatgg tcagttatta cttactgatg
    20941 cagttttcaa cttatttgga atatgaatta gccatcaata ctgttattta ccggcaattt
    21001 ttgcttgttg tttattggat tatatttacg ggagcaatag ccagtttaga ccggagactt
    21061 cgaaattctt tacaggagag gttagctgtt caaagagaac tccaattggc taatatgata
    21121 gattatagcc accatattga ggggttgtat aaagaagtta ggagcttccg acatgattat
    21181 agtaacatgt taacgacctt gaaactaggg attgataatg aagatatatc tattgtcaaa
    21241 tcagtttatc agtctatctt taaagaatct aatacacatt tccgacatcc taagtataat
    21301 atagaccggt tggttaatgt agataaccca gccttaaaaa gtcttttagc agccaagctt
    21361 tcacaagcga tagacaatca tattatggta tctatagaag taccagacag tattaaacct
    21421 caagggatgg aactgattga ttttattacc attgtatctg ttttatggga taacgctatt
    21481 gaggcatctt tagagacaga cgatcctaag atagccatgt cttattttac attaaaacat
    21541 aaacaaattt ttatcataga aaattcaact aaggaaaagg caatagatac gtcaacgata
    21601 ttcaattatg agataagtag taaggggcgg aatagaggaa taggtcttta caatgtcagg
    21661 ggagttatca atcgttatcc aaatatttct attgcaacaa gtagccaaga ttttagtttc
    21721 tgccaaactt tcgaaatcat attatagttt taaaaagttc aatttatcta agtttattat
    21781 tttcttaata ccatcaatcc aaccaccacc agtgatatta tttagttgtt tcggcgataa
    21841 ttcctgaagc tgtagttcca tctcgattag ttttggctag ttctcgtaag tggtttaata
    21901 aatattttga accatagaac tttgcgattg aagcaagtgc agttactcca caatccttag
    21961 tatcaacctg acgcaccaat gcttttttgt acgtcaacat tgtgtaattc ctttcttcgt
    22021 tatcgtatta atattttgcc ataaaaatat atacttatta aaaatagctt aaacagctat
    22081 tgcaacgcgt ccaatggttt ctataaggat ataaaggtag atagtacttt tctgttttga
    22141 ggcgattcaa cgacatttca gggaataaac gtttctattt ttatttgaat gctatattaa
    22201 atgtgtcaag ttgcaacaga caaaaatttt atataggaga tattcaaaat aaatgtaaaa
    22261 tcaattgaac aattagaagt tatggataat gaagcattat cacatgttga aggtggcctt
    22321 ggtcatagat gggtatacag taagaaccgg taaacatact tatacaagtg catggcatct
    22381 ttctgaaaat actgctgcag caaatgcaag aaatcgtatg ggcctttacc ctggaagcgt
    22441 ttgtcgtgtc tatcttgtat aattgatttt ttatttaagc gatagggaat cttccttgcc
    22501 gcttaagttt gtatagggag agacaatact atgttattac agctagataa tatcactaaa
    22561 agctacggaa aaaaaatagt acttaaccag atttcctacc agtttactcc tggtttgtat
    22621 ggactcttag gagcaaatgg tactggcaaa acaaccttat taaatttgat gagccacttt
    22681 acattcgcag atagtgggaa tatttattgg aatggacagg agcagtcgga agaattttat
    22741 cgtcacattg gttttttacc gcaacatttt cgctattatg atcaatttac tggcattgct
    22801 ttcttaaatt atatcgctac cttaaaaggt gttgataaaa agaaagctaa accagaaatt
    22861 cctagattac tagagcttgt tggtttaggt gatgtaggta aaaagaagat ttcatcatat
    22921 tccggtggta tgaagcaacg tttaggcatt gctcaagcac ttatcaatga ccctgaaata
    22981 cttattctgg atgaaccaac tgttggactt gatcctaagg aacgggttaa atttcgccat
    23041 attttgagtc agttatccac caataaaatt atcattttat caacgcatat tgtgtcggat
    23101 gtggaagctg ttgccaaaga aattatagta ttaaaaaatg gtaaatttat cgaacagggc
    23161 aacaccgcac agttgttgaa aactattgag ggaaaagtgt gggaaataac gactgaacca
    23221 gatttatctc agatacccaa tattgctatt gttaatgaaa aagtcatttc agatagtctg
    23281 gtatttcgtg tagtgagtga tatttgtccg agtgattcgg cacagttggt tgttccgact
    23341 ctagaagatt tctacatcta tcattttaga ggggagtaag taagatgata tcaatttttc
    23401 cttttgaatt aaaaaaactt ctacaacgta aggcagcatt gggggctttt attatcttcc
    23461 ttcttgctat ttttggtcta ttttaccagc atttttttgt tgggcaaatt agtggctatt
    23521 ctgcagataa aatacatggt cgagaagcgg ttgcaattaa tcgtagaata gctgaaaaat
    23581 attcagggga tttatctgaa gaacgagtaa caaatattat taaaaactat gttaatccgc
    23641 cagcaggacg cccccctata tttgatgttt ttagttatta tgtggcagac acttttg
//
LOCUS       DATRME010000025        23366 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRME010000025 DATRME010000000
VERSION     DATRME010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23366
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..534)
                     /locus_tag="VBC23_000986"
     CDS             complement(<1..534)
                     /locus_tag="VBC23_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6842943.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQI"
     gene            630..1349
                     /locus_tag="VBC23_000987"
     CDS             630..1349
                     /locus_tag="VBC23_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6842944.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            1422..2573
                     /locus_tag="VBC23_000988"
     CDS             1422..2573
                     /locus_tag="VBC23_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6842945.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            2625..3572
                     /locus_tag="VBC23_000989"
     CDS             2625..3572
                     /locus_tag="VBC23_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6842946.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            3588..5393
                     /gene="pepF"
                     /locus_tag="VBC23_000990"
     CDS             3588..5393
                     /gene="pepF"
                     /locus_tag="VBC23_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6842947.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            5588..6214
                     /locus_tag="VBC23_000991"
     CDS             5588..6214
                     /locus_tag="VBC23_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO6842948.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            6288..6995
                     /locus_tag="VBC23_000992"
     CDS             6288..6995
                     /locus_tag="VBC23_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6842949.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            7056..7985
                     /gene="prsA"
                     /locus_tag="VBC23_000993"
     CDS             7056..7985
                     /gene="prsA"
                     /locus_tag="VBC23_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6842950.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            8227..8712
                     /locus_tag="VBC23_000994"
     CDS             8227..8712
                     /locus_tag="VBC23_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6842951.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            8728..11346
                     /gene="alaS"
                     /locus_tag="VBC23_000995"
     CDS             8728..11346
                     /gene="alaS"
                     /locus_tag="VBC23_000995"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6842952.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            11428..12144
                     /locus_tag="VBC23_000996"
     CDS             11428..12144
                     /locus_tag="VBC23_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6842953.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            12232..13050
                     /locus_tag="VBC23_000997"
     CDS             12232..13050
                     /locus_tag="VBC23_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6842954.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            13251..13439
                     /locus_tag="VBC23_000998"
     CDS             13251..13439
                     /locus_tag="VBC23_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842955.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            13471..13764
                     /locus_tag="VBC23_000999"
     CDS             13471..13764
                     /locus_tag="VBC23_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6842956.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            13809..14024
                     /locus_tag="VBC23_001000"
     CDS             13809..14024
                     /locus_tag="VBC23_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6842957.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            14027..14785
                     /locus_tag="VBC23_001001"
     CDS             14027..14785
                     /locus_tag="VBC23_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6842958.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMTFVSISIVLSIFNTLFNYKLNIFDDSVTLVIPIYDLSLLFVLLGLHIYFLKVYR
                     NIRGIKMTVAPTLKELKNNVLQLDEAELESNYKMCFDIVMNLSGFIFPTIYFVLFFIS
                     FVFQKVEIVAIIITTSIHIYILIKSLKAARHFYR"
     gene            complement(14870..15433)
                     /gene="thiT"
                     /locus_tag="VBC23_001002"
     CDS             complement(14870..15433)
                     /gene="thiT"
                     /locus_tag="VBC23_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6842959.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
     regulatory      complement(15490..15588)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15673..16632)
                     /gene="nrdF"
                     /locus_tag="VBC23_001003"
     CDS             complement(15673..16632)
                     /gene="nrdF"
                     /locus_tag="VBC23_001003"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6842960.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     gene            complement(16835..18994)
                     /gene="nrdE"
                     /locus_tag="VBC23_001004"
     CDS             complement(16835..18994)
                     /gene="nrdE"
                     /locus_tag="VBC23_001004"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6842961.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            complement(19072..19296)
                     /locus_tag="VBC23_001005"
     CDS             complement(19072..19296)
                     /locus_tag="VBC23_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO6842962.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            19679..19942
                     /locus_tag="VBC23_001006"
     CDS             19679..19942
                     /locus_tag="VBC23_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6842963.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            19947..21680
                     /gene="ptsP"
                     /locus_tag="VBC23_001007"
     CDS             19947..21680
                     /gene="ptsP"
                     /locus_tag="VBC23_001007"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6842964.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            21830..23257
                     /locus_tag="VBC23_001008"
     CDS             21830..23257
                     /locus_tag="VBC23_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6842965.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
BASE COUNT         7723 a         3763 c         4502 g         7378 t
ORIGIN      
        1 aatttgtcct tgtaaggaat cgggatagta atcgtcagta tttccagtaa tatgattaaa
       61 tgcagtattt aacatgcgca taatttcaga tgattcattg ttaacgattg tatggcattt
      121 tttatcccaa agaacaggga ctgtcacgcg tcctgtataa tcactttgac ttgcttgata
      181 gatttggtaa aggtattggc tttggttaat catatcagga ataacgcctt tatattcttg
      241 aaaagtccaa ccattctcca acattaaagg gttaacaatg gagatagaga tatgagattc
      301 tagattttta agctttctca tgattaatac acgtgatgcc caagggcagg ctagtgaaac
      361 gtagagatga tatcgtccag attctgcttt aaaacctgca tccccactag gccctgctga
      421 accatcttta gtaacccaat gacggaattg tgtaacggtt cggacgaatt taccaccagt
      481 tgatgcagta tcataccatt gatcaaccca tttgccatct actaaaagtc ccatgataat
      541 ctcctttgtt tttctattac taattagtag tataaagatt ttaaaaggaa ttagcaaatg
      601 tttaacttac agtatggtaa actaagggta tgcgtttaga taaattatta ggtcaggcag
      661 gatttgggtc tagaaatcag gttaagaaat taattcgttc taggcaagtt tctgtagatg
      721 gtcaaattgt tacaaaggat aatgttattg tagatagtgg tctacaatcg atttttgtag
      781 gaaaagagag agtctgccta aaagaaagtt cttattacct tctttataag ccatcaggag
      841 ttgtttcagc agttagagat tcagaacata aaacagtcat agacttaatt agcgaaaaag
      901 ataaggtgga gggattgtat ccgattggtc gtctagatcg tgatacggaa gggcttttaa
      961 taatcacgaa taatggtcca ctgggttacc gaatgttaca tccaaaacac cacgttgcaa
     1021 aaacgtatta tgttgaagta aatggtttct tggaaagaga tgctattacc ttttttgaag
     1081 aaggagttgt atttgacgat ggaacaaagt gtaaaccagc tgaattaacg attgatacgg
     1141 ctaataatga caaaagtacc gcaagaataa ctatcacaga agggaaattt caccaagtaa
     1201 aaaagatgtt tcttgcatat ggcgttaaag ttatatactt gaggcgtatt tcttttggtg
     1261 acttgaggct tgatatgaat ttgaaaccag gtcagtatcg tagattgaga gattcagagg
     1321 cagctatctt aaaaaggtat ttagattagt gaaacgactc acttgacctt gcttagtatt
     1381 cttcatatac ttataagtga ttattttaaa ggagaaaact attgaattca aaactttcat
     1441 tgaaattgac tattttatcg atttctatct ttttgatgtc tcacttagca attgctcctg
     1501 caattcctaa attgtatgcc ttttaccata ctcaaaaccc tcatattgga ctagcttcag
     1561 tagagagttt agttactatt ccagctatga tgattactat ttttgttatt ttgagtaatt
     1621 ttgtagtcac aaaacttggc aaaaaaaaca cggtcttact tggtttatgt ttaatcttaa
     1681 tgtctgggtt tatctcattt tttacaagta atttttcact tgcgatggct agtcgactac
     1741 ttttgggtat tggtattggt ctctataatt cgctatcaat tagtatcata acggatttat
     1801 atgaggctga tgagagagcc agtatgattg gtcttagaac agcatcactt aatatcggta
     1861 aagccttaac tacatttatt gttggattgg ttttagctat tggtgtgaat tatatttatt
     1921 tggtttacct tctggttatt cctgttttct ttttcttttg gaaaaatgtt ccagaggttg
     1981 aaaatcaaac acataccctt aaagctagta caacatttga tactaaagcg gctttgttga
     2041 tgctcatcac gttcttagta ggtattgctt atataggagc aactgtcaaa attccaacct
     2101 tactagtgac aaaatatcac tacgcgacga gcttctctag taatatgtta actctccttg
     2161 cttttagtgg tattttagtt ggatctgtat ttggaaaatt agtcaaagca ttccaagaga
     2221 agacgctatt aatcatgatt ctagctatgg gaataggtaa tgtgctattt gctttagcta
     2281 ataatcagat catttttatc gtagctagta ttttaatagg agctagtttt gttggcacca
     2341 tgtcttctgt attcttttat atttcaaaaa actatgcaaa agaacataat aactttatca
     2401 caagccttgc cttaacagct ggtaatatag gagttatttt gacaccgctt attttaacga
     2461 aacttccaag ccagctacat ctggaacctt ttataacacc atttttaata acaagtgggc
     2521 tgatggtgat taatgtcttt gtttacctag ttttaatgtc aaaaaataaa taaaaagtta
     2581 tccgaaagga tagctttttt cgaatagtaa aagtaggaga aaaaatgtta attgctaaag
     2641 acaaacaagg aaatttaatt aatctactcg agtctcatcc gggaaaggga cagtattttt
     2701 gtccaacttg ttgttcagct gtaaggttaa aagcaggtcg aatcatgaga agacactttg
     2761 cacatattag tctaaaaaat tgtcagtttc atcatgaaaa tgagtctaat gagcacttgc
     2821 aattaaaagc aaagttatat atgagtttaa gtagagagaa tgaaactatg ctcgagcatc
     2881 atttgccaga aattaatcag attgcagatt tatttgtgaa tgagacattg gctttagaag
     2941 ttcagtgtag ccgactatca gagcagcgac tcagggagcg aacaaaagca tatctacagg
     3001 cagactttca agtgagatgg ttactagggg aaaaactctg gttaaagcat cgtttaacaa
     3061 atctacataa acaattttta caattttctc aaagtatcgg atttcatatt tgggagttag
     3121 atttgaggtt agaagtactg agactaaaat atttgattta cgaagattta cgcggacatg
     3181 tctattactt aagtaaaact tgccctctta gtggagacgt tctagcattt ttgaagtggc
     3241 cataccaatc aaaaaatcta aatttttata aggttaaaca agacagaaat atcagagatt
     3301 atgtgcgtca gcagttacgc tatggcaatc aattttggtt acgaaaacaa gaaaaggcct
     3361 acctttctgg tcagaatcta ttaacccaag agctaatgat gttttttcct caaatacagc
     3421 caccacgtgt agatactgat ttttgtcaaa taacaaattc tctgacatcg ttttatcaga
     3481 attttacaaa ttattatcaa aaaaataaga ataacttgga ccaaacactt tatccccctg
     3541 tattttatga taaaatagtc actaagaatt aaactggagg atacaaaatg tcagacaatc
     3601 gctcacatat tgaagaaaaa taccaatggg atttaactac tgtctttgcg actgatgagt
     3661 tatgggaaac tgaagtggta gagcttactc aagccatcga tgacgcaaag ggtttttcag
     3721 gtcatctgtt ggactcaagc caatctttat tggagattac ggaggttgaa ctagagttat
     3781 ctcgccgttt agaaaaagtt tatgtctatg cttcaatgaa aaatgaccaa gacaccactg
     3841 ttgcgaaata tcaagaattt caagcgaaag cgacagcgct ctatgcaaaa tttagtgaaa
     3901 cattttcgtt ttacgagcca gaactattgc aactttcaga gtctgattac cagtcattcc
     3961 ttttagaaat gcctgacttg caaaaatatg atcatttctt tgagaaaata tttgcaaata
     4021 aacctcatgt cttatctcaa aacgaagaag aattgttagc tggtgctagt gagatttttg
     4081 gagcagctgg tgagacattt gaaatccttg ataatgcaga tatggtcttc ccagttgtaa
     4141 aaaatgcaaa aggtgaggaa gttgagctta cacatggtaa tttcatttcc cttatggaat
     4201 ctagcgatag gacggttcga aaagaagcat atcaagctat gtatagcaca tatgaacaat
     4261 tccagcatac ttacgctaaa acattacaaa caaatgtgaa atctcaaaat tttaaggctc
     4321 gtgtgcatca ttatcaatca gcacgccaat cagctctatc tgccaatttt attccagaag
     4381 aggtctacga aactctaatt aagacagtta atcatcattt accgctctta catcggtata
     4441 tgaagttgcg tcaaaaagtt cttgggctag atgatttgaa gatgtatgac gtatatacac
     4501 ccctttctca aatggatatg agttttactt atgatgaagc tctaaaaaaa tcagagaagg
     4561 ttctggctat ttttggcgaa gcatattctg aacgtgttca tcgtgctttt actgaacgtt
     4621 ggattgatgt ccatgttaac aaaggaaaga ggtcaggcgc ttattcaggt ggatcttatg
     4681 acactaatgc tttcatgctt cttaactggc aagatacttt agacaatctt tatacgctgg
     4741 tgcatgaaac tggacatagt ctacactcga cgtttactcg tgaaaatcaa ccttatgttt
     4801 atggtgatta ttctattttc ctagctgaaa ttgcatcaac aacaaatgaa aatatattaa
     4861 cagaaaccct tttgaaagaa gttaaagatg ataaaaatcg ctttgcaatt ctcaatcatt
     4921 atttggacgg tttcaaagga acaattttta gacaaacaca gttcgctgaa ttcgaacatg
     4981 ccattcatgt agctgatcag gaggggcaag tactaactag cgaatactta aacaaccttt
     5041 acgctgagct taatgaaaaa tattatggtt tgactaaaga agataatcat tttattcaat
     5101 atgaatgggc aagaattcca cacttttact acaattatta tgttttccaa tatgcaaccg
     5161 gcttcgcagc agcaaattat ttagcagagc gtattgtaaa cggtaatccg gaggataaag
     5221 aggcctacct aaattacctt aaagctggga attcagatta ccctctaaac gtcattgcta
     5281 aagccggtgt tgatatgact agtgcagact atctggatgc agcttttaga gtatttgaag
     5341 agcgtttagt ggaactggaa aatcttgtag caaaaggcgt ccataatgat taagttgaaa
     5401 atatagctat tgaagtaaaa ccagtcaact agactggttt ttgatagtga tcagttattt
     5461 tatagtttgt tttgagagtg gttttggtaa agttagttag tggaaaactc aataggtgga
     5521 aatacctacc caagctatga tttttatgag accaaaactg ctattgtcaa aaaagaaggg
     5581 aaataggatg aaaaagctta cttttatttg ggatttagat gggacattaa tagattcgta
     5641 tgtaccaatt atggaagctc ttgaagaaac ctatcgtcat tttggcttaa tatttgataa
     5701 agaattaatc catgaatata ttttacagga atcagtgggg caattattgg taaacctttc
     5761 agaggaagag caaatacctc atgaaaaact gaaagcatat tttacaaaag aacaagaaag
     5821 tcgagattct aaaatacatt taatgccata tgcaaaagag attttagaat ggaccaaaga
     5881 acaagatatt cccaatttta tgtatacaca taaaggagca agtacgcatt cagtgttgga
     5941 aaccttgcag atctctcatt attttgatga aattttaact ggtgtttcgg gattcgagcg
     6001 aaaaccacat ccacaaggga ttaattattt agttaaacga tattctttag ataaatcaat
     6061 gacttattac ataggagatc gtccactaga tttggaggtt gctcaaaatg ctggtataaa
     6121 atccataaac ttaaggttag agaattccaa agaaaactat aatatttcaa gtctcaaaga
     6181 tataatatca cttgatttca ctcgtttgga ttaaatcatt ctcagagtta gacatttttt
     6241 gctataataa aaaagttatt atttaaaata gaaaagagga gccaaaagtg gttcaatctt
     6301 acagtaaaaa tgctaatcat aatatgcgcc gtccagtagt taaagaagaa attgttcaat
     6361 atatgcgtca gcaccaaaaa caaaataatg gctgtttagc agagttagaa gcatttgcta
     6421 aacaggaaaa tatccccatt atacctcatg aaacagcgac ttattttcgt tttttgatgc
     6481 aaacattaca acctaagcac attttagaaa tcggaacagc tattggtttt tcggcccttt
     6541 tgatggcaga aaacgctcct gaagcaaaga taacaacaat cgatcgtaat gaagagatga
     6601 ttgccttggc taaggaaaac tttgcaaaat atgataatca caatcagata acccttttgg
     6661 aaggcgatgc tgtggatgtg ttacaaacat tggataaatc ttacgatttt gtatttatgg
     6721 attctgctaa gtctaaatat atcgtttttt tacctcaagt attaaagcat ttagatgttg
     6781 gaggagtagt tgttttagat gatattttcc aaggtggaga tattgcaaaa cctatcgatg
     6841 aagtgagaag gggtcagcga actatctacc gaggtctgca acgtttattt gactcgactt
     6901 tgcaacatcc tgatttaact gctactttgg tacctttggg agatggttta ttgatgattc
     6961 gtaaaaatgc agatcatatt gtactagaag attaaggcat ttttaagaaa ttatgatata
     7021 ataatggagt taatgacaaa aaggagttat aaatcatgaa aacaagatca aaacttgcag
     7081 caggcttttt gacacttatg tcagttgcca cattagctgc ctgctcaggt aaaacatcaa
     7141 atggaacaaa tgttgttaca atgaagggcg acactattac agtctctgat ttttatgatc
     7201 aagtaaaaac atcaaaagct gcacaacaat caatgcttac attgatcctc tcacgtgttt
     7261 ttgatacaca gtatggtgat aaagtttcag ataaaaaagt atcagaagct tataataaga
     7321 cagctaaagg ctatggtaat tcattttcaa gcgcactttc acaagcaggt ttgactccgg
     7381 aaggttacaa acaacaaatt cgcacaacta tgcttgtgga atatgctgta aaagaagcag
     7441 ctaagaaaga attaacagaa gcaaactata aagaagcata taaaaactat actcctgaaa
     7501 cttctgtaca agtaatcaaa ttggatgcag aagataaagc taaatctgtc cttaaagatg
     7561 taaaggctga tggagctgat tttgcaaaga ttgcaaaaga aaaaacaaca gctactgata
     7621 aaaaagttga gtataaattt gattctgcag ggacaagcct ccctaaagaa gttatgtcag
     7681 cagcctttaa gctagataaa aatggtgttt cagatgtggt ttcaacggtt gattcaacaa
     7741 cttataaaac aagttactac atcattaaag taactgataa gacagagaaa aaatctgatt
     7801 ggaaatctta caaaaatcgc ttaaaagaag ttattcttaa ggataaaaca agcgatagat
     7861 ccttccaaaa taaagtgatt tcaaaagcct tagaaaaagc taacgttaag attaaagata
     7921 aagcatttgc aggcatctta tcacaatatg ctacaacaag tggttcatca tcacttaaaa
     7981 aatagttgac caagtcttaa aaagactgta taattaatac tattgagaat gaaatttcaa
     8041 cgattagata cagaaaagtg acggttgctg cgagtcatgg ataatcagaa aaagatgcta
     8101 ctcaatgggg taaaactaaa aataaatgag aatgacaagt tcattgaatg aaggtggtac
     8161 cgcggttttc gcccttcgta atggacttgt cttttgctat ttgtcaactt tcgaggagga
     8221 agagatatgg ctacttttca aattaaagaa aaaatgtttt ctctaggcgg taaatttact
     8281 attacagacc tgactgggct tccctgttat cacgttgaag gaagtttgtt ccctctacct
     8341 aagactttta aagtctttga cgaagaggaa catctcatta gtcagattga gaagaaggtt
     8401 ttaagttttt taccaaagtt taatgtgaca ttagccaatg ggaatcattt cacgattaag
     8461 aaagatttct catttttgaa acctcattac accattgaag accttgatat ggaagttaag
     8521 ggaaattttt gggatatgga tttccaactc ttaaaagaca atcaagttat agcaaacata
     8581 tctcaacagt ggtttcgtat gacttcaact tatcaggtag aagtttacag tgaaacttac
     8641 aacgatttga caatttcgct tgtcatagcg atcgactatg tcaaagagtt agaaaaaaat
     8701 gcatcaaact aattttagga gagaataatg aaagaattat cgtctgcaca aatccgccaa
     8761 atgtggttgg atttctggaa atccaaagga cattccgttg agccttcagc taacttggtt
     8821 cctgttaatg acccaacgct tctttggatc aactcaggtg ttgcaacctt gaaaaaatat
     8881 tttgatggtt cagtgattcc agaaaatcca cgtattacca atgcacaaaa atcaattcgt
     8941 actaatgata ttgaaaatgt tggtaaaaca gcacgtcacc atactatgtt tgaaatgctt
     9001 ggtaacttct caattggaga ctatttccgt gatgaagcta ttgagtgggg atttgaactc
     9061 ttgacaagtc ctgaatggtt tgacttccct aaagacaagc tctacatgac ttattaccca
     9121 gatgacaagg attcatataa ccgttggatt gcttgtggcg ttgaaccaag tcacttggtg
     9181 ccaatcgagg ataacttctg ggaaatcggt gctggtcctt caggtccaga tactgagatt
     9241 ttcttcgacc gtggtgaaga ttttgatcca gaaaatattg gacttcgtct tttggctgaa
     9301 gatatcgaaa acgatcgtta catcgaaatc tggaacattg ttctctcaca attcaatgct
     9361 gacccagccg taccacgttc agaatacaaa gaattgccaa acaaaaacat tgatacgggt
     9421 gcgggtcttg aacgtcttgc agctgttatg caaggggcaa aaacaaactt tgaaactgac
     9481 cttttcatgc ctatcatccg tgaagtagag aaattgtcag gtaaaactta cgatccagat
     9541 ggcgacaaca tgagcttcaa ggttatcgct gatcacatcc gtgcgctttc atttgccatc
     9601 ggtgatggtg cgcttcctgg aaatgaaggt cgtggttatg ttcttcgtcg tcttctccgt
     9661 cgtgcggtta tgcacggtcg ccgtcttggc atcaacgaaa ctttccttta caaattggtt
     9721 ccgactgttg gacaaatcat ggaaagctac tatccagaag tgcttgaaaa acgtgatttt
     9781 attgagaaaa tcgttaaacg tgaggaagaa acatttgctc gtactatcga tgctggtagc
     9841 ggtcacttag attcattgct tgcgcagctt aaggctgaag gtaaagacac acttgaaggt
     9901 aaagatatct tcaaacttta tgatacttat ggattcccag ttgaattgac agaggaattg
     9961 gcagaagatg caggctataa gattgaccac gaaggcttta agtcagccat gaaagaacaa
    10021 caagaccgtg cgcgtgcagc tgttgttaag ggtggttcga tggggatgca aaatgaaacc
    10081 ctagctggta ttgttgaaga atcacgattc gaatacgaca catatagtct tgaatcaagt
    10141 ctttcagtta tcattgctga taatgaacgt actgaggctg tttcagaagg tcaagccctt
    10201 cttgtctttg ctcaaacacc attctatgct gaaatgggtg gacaggttgc tgacacaggt
    10261 agaatcaaaa atgataaggg tgacacagtt gctgaggttg ttgatgttca aaaagcacca
    10321 aatggtcaac ctctacacac tgtaaacgtt ttggcatcac tttcagttgg aacaaactac
    10381 acacttgaaa tcaacaaaga gcgtcgtttg gctgttgaga aaaaccacac agcaactcac
    10441 ttgctccatg cagcccttca caatgttatc ggtgaacacg caactcaggc tggttcattg
    10501 aacgaagaag aattcttgcg ctttgatttt actcactttg aagcagtaag caatgaggaa
    10561 cttcgtcaca ttgaacaaga agttaatgag caaatttgga acgctcttac aatcacaacg
    10621 actgaaactg acgttgaaac cgcaaaagag atgggagcaa tggcactttt tggtgagaaa
    10681 tatggtaaag tggttcgtgt ggttcaaatt ggtaattatt ctgttgaact ttgcggtgga
    10741 actcacttaa ataattcttc agaaatcggt ctcttcaaga ttgtcaaaga agaaggtatt
    10801 ggttcaggca ctcgtcgtat tattgcagtt actggtagac aagcttttga agcttatcgt
    10861 aaccaagagg atgcactaaa agagatcgct gctactgtaa aagctccgca attgaaagat
    10921 gcaacagcta aagtacaagc tcttagcgat tcacttcgtg atcttcaaaa agaaaatgta
    10981 gagcttaaag aaaaagcagc agcagcagca gctggtgatg tctttaaaga tattcaagaa
    11041 gctaagggcg tgcgcttcat tgctagtcaa gttgatgttg cagacgcagg tgcacttcgt
    11101 acatttgctg ataactggaa acaaaaagac tactctgatg tgcttgttct cgtagcagct
    11161 attggtgaga aggttaatgt ccttgttgca agcaaaacta aagatgtcca cgctggtaac
    11221 atgatcaaag gattggcacc aattgtagca ggtcgtggtg gtggtaaacc agacatggct
    11281 atggcaggtg gtagcgatgc aagtaaaatt gcagagctgc tagcagcagt tgcagaaaat
    11341 ttataagaag tacctctcat ttagttagta aataaaacag gcaaagggca gaacatcgtt
    11401 ctgttccttt gtgtatatag gagaaatatg gttggtttag gaacagttat caatgttatt
    11461 cttattatag ttggaggctt tgtaggttta tttttgaaaa attttttgaa agaatcactt
    11521 caaaagagcc tcatgcaagc catgggagtc gcagttttat ttatttcaat atcaggtgtt
    11581 ttggagaaaa tgatgttagt cgaaaagtca catctcatct ctaatcatac aaatatgatg
    11641 attattactc ttgctctagg tacggtactt ggagagttgc tatcactaga ttcgtatatt
    11701 gatagatttg gggactatct taagcaaaaa acaggaagtg gtaacgatat taaatttgtg
    11761 gaagcattcg tcacctcaac ttgtactgtt tgtattggag caatggccgt tgttggtagc
    11821 attcaagatg gtatagcagc agatcattcg attttatttg ctaagggaat gttagacatg
    11881 attattattg caattatgac agtgtcttta gggaaagggg ctctattttc tgcacttcct
    11941 gtcgctttac tacaaggtag ccttacgatt gttgcttttt tcatggggag cctacttaat
    12001 ccatcatctt tagattacct taacttagta ggaaatatgc ttatattctg tgtcggtgtt
    12061 aatttactct ttaacctaaa tattaaagtt atcaatatgt tgccagcaat tattttagct
    12121 atattatggg gaagttttat ataaaagata accaagtttt aactcaaact tggtttttct
    12181 ttagacatat gttaggatga aagaagatat actacggaga ggatccaact catgaatcta
    12241 ttattttcta ttgatgatat gtacgtagat cattttaaag tgatgcttta ttcgcttgtc
    12301 cgacaaacca agaatcgtaa gttagagata tatgtcttac aaaaaacatt actcaaacgt
    12361 catacagaac ttattcaata tactcaaaat ttagaagttg gctatcatcc aataattgta
    12421 ggtaccgaag tatttgctca ggcacctaca actgatcgct atccagatac aatatattat
    12481 cgacttttgg ctcataaatt tttacctgag accttagata ggatacttta tttagatgca
    12541 gatatgcttt gcttaaatga tttctcaagt ttatatgaca tggagttagg tgatcaacta
    12601 tatgcagcgg caagtcataa tactgatggt aagtttctag attatgtcaa taaattacgg
    12661 ttaaaaaatg tagagctaga atcctcttat ttcaatacag gggtactctt gatgaacttg
    12721 cctgctatac gaaaagttgt tcatcaacaa actatcttag attacataat gcaaaaccgt
    12781 ggtcgtctaa tcttaccaga tcaggatatc ttaaatggct tatacgctaa tttggttaaa
    12841 ccaatacctg atgaaattta taattatgat gctaggtata gtcttattta tcaattaaaa
    12901 agtcgcaatg aatgggattt agagtgggtt attaaccata ctgttttttt acattttgca
    12961 ggacgagata aaccatggaa aaaagattat agaggacgtt actcgggcct ttataagttt
    13021 atggctaaag aggctttaac taaaaattga agatttaaaa ggttactaaa ttatttctat
    13081 ttagtaactt tttttggggt gatattttaa tgatatcaat aatctttgct aataattatt
    13141 tataaaaaag aaaaataata tcaaaggagg agcgtgttaa aatcacttaa tcattgaaca
    13201 atcaaattaa tcattgtttc aaaagcataa tggttttagg agttactcaa atgaaaaagg
    13261 aatttattat tacaggtcgg atggttgcaa gagttatgga aaccctagtt tgcttatgta
    13321 tggtaagtaa tcttgttctt gatatgcttg atataataga aataaatgaa attgtatttt
    13381 gtgtgttggt agcatcaatt gcagggcttg agttttgttt gcaaaaagaa agggagtaga
    13441 tagatggaat ttttggctta atggcttttt atggtcatga cgtatattgg tgtacctaaa
    13501 gagcaaaagg cactcagtcg taggggtaaa caattagaaa aaataaatca gggaggaaaa
    13561 acacttatgt cacaaatgat tcaagattta gtaggaagca catgtatgat ttatttagag
    13621 gatggttttc aaacgcatcc ttatgagatt ttaaaggtag atgaggattg ggtacagatt
    13681 cgtcgcaact tgaaacgaag taaagaggag gtacgtattc tccgtattca tgatatcaag
    13741 gaattgcgtc cagtcaataa atagagtgag gaataaatct atatatcatt ttataaagga
    13801 gaaaaatgat gggattaatt ttaaaaaaca ggctcaaaga attgcgtgca cgagatggta
    13861 tcaatcaaac agaactcgcc aaattaacgg gagtctcaag acaaacaatt agcctaattg
    13921 aacgcggaga atatacgcca tctgttatta ttgctatgaa gatttctcaa gtatttaaag
    13981 aatccctaga gaatgttttt agtttagtgt caacggagga ataacgatga aaaattttat
    14041 aaaacaaagt gatactacat ttgttcgaat tatcaaaagt cttttaattg gtggttttat
    14101 aggggccata cttggttccg taggagctct ttttaacata tttggtcaag ataagtactt
    14161 atctgaaatt aatattgttc aatatttcct atgggttagt cgtattgttg taattataac
    14221 agctctcttt agtctcatct acctctatca gattcaaaaa tatcagaaag tttttttcaa
    14281 tgttgatgaa agtcaatctg aagaaattta tcgccagatt aatttaagac attcttatgg
    14341 gatgactttt gtgtcaattt caatagtact cagtatattt aatactttat ttaactataa
    14401 actaaatatt tttgatgatt cagttacttt agttattcca atatatgatt tatcactgtt
    14461 atttgtttta ttgggtttac atatatattt tttaaaggta tatcggaata tccgaggtat
    14521 aaaaatgaca gtcgctccaa ctttaaaaga gttgaaaaac aatgtcttac aattggacga
    14581 agcagagttg gaatcaaatt ataagatgtg ttttgatatt gtcatgaatt taagtggttt
    14641 tattttccct acgatctatt ttgtattatt ctttatctca tttgtttttc aaaaagtgga
    14701 gattgtagca attattataa caacatctat tcatatctac attctcatta agtctttaaa
    14761 agcagcacgt catttttata gatagtagta gctagatttt ttatcaaaaa acttatatta
    14821 gtgtaaaata aaaggattga ttctctgaaa atcagtcctt ttattttttc tatttcttag
    14881 gtaaaaagaa gctagggtag ctgataacta aaataataat actaatgact acaaaaaata
    14941 gagttaacaa accagcagta ccattgacgc ttaatgagta taatgtagca gacatacctt
    15001 ttggtgcata actagcccaa aaaataacac cagcgatata atgccaaaca taacgaacaa
    15061 atgttgctaa tattgctcca gataatgcta atgataaagc tttagtttta gagctgctgc
    15121 taagtgcatc tttaaattta gcagaaaata cgcctgcaag tcccatactt ataaatgcta
    15181 agatgtattc tataaaaact tgggataaac taaggtaata tactttggaa agaacaaagt
    15241 gcaataagcc ccaaattaag ccagcaaata gaccgtattt tgtaccacga cgtaatgcaa
    15301 aaagtatcag aggaattgct ccaaaagagg gagtgaacca tgacgcaaaa tcaggaatca
    15361 ttgataaagc catagcaagc gctgcaaaaa tagcagtttc aataagacag gtagtgttgt
    15421 tattttttga cataaaaaat ctcctttttt atacagcaaa ggagattctt ttgtcatttg
    15481 tcatgtttaa ccaattcaaa ccacaattcc tacgctcgtc ctaacgagat caggttagag
    15541 gattttgcat atctgcaatc tcagctcaag gcactccctt gtgtatttac tctaacattc
    15601 tatcttgaaa gggtttgctt gtcaagaaat ttgtatttaa ctagaaaaag acactaaatc
    15661 agtgtctttt tcttataatc catagttata gtcatcatca tgcatagctt caacgctacc
    15721 aagtaggtaa ccattaccta cttgagagaa gaagtcatgg tttgatgttc ctgttgaaat
    15781 accattcata acaattgggt tgacatcatt tgctgtatct gggaataaag gatcttgtcc
    15841 taaattcata agagctttat tagcattgta gcgtaaaaag gtcataactt cttcagtcca
    15901 tcctacgcca tcataaagtg tcttggtgta tttttcttcg ttttcataca gctgataaag
    15961 gaggtcatac atccaatcac gaaaattctc ttgctcatct tctggtaatt cgttaaaacc
    16021 aagctggaat ttgtaaccga tataagtacc atgtacagat tcatcacgaa taattaattt
    16081 aatgatttca gcaacatttg ctaacttatt atttcccaaa tagtaaagag gtgtgaaaaa
    16141 gccagaataa aaaaggaaag tttcgaggta ggtggaagcc accttttttt gaagggcatt
    16201 tccattagca taaatgtcat tgataatacg tgctttttct tgaaggaact cattattatt
    16261 agtccactcg aaaatttctt caatttctga tttagtattt aaagttgaga aaattgaaga
    16321 ataagattta gcgtgaacag attccatgaa ttgaatattg tttaagacag cttcttcgtg
    16381 aggcgtgcga acatcggcac gaatagcttc aacaccagtt tctgattgca tggtatcaag
    16441 tagggttaag cctccaaaaa ccttgccaac aagatctttt tcttgagcgg aaagtttgcg
    16501 ccaatcgtct aagtcatttg ataaagggat acgtgtatcg agccaaaatt gttcggttag
    16561 tttttcccaa gttgatttat caataacatc ttcaatttcg ttccagttta tagcttcgta
    16621 gtaagttgtc atggttttct ccaatggtaa ataggttttt atataatgat gatggaagaa
    16681 gagtagaata tagaattctg atctagcaat atttcaagta ttatcgattt ttagaaaaat
    16741 aaagatgaaa ataccttaga aaataagata acatcatgaa actgagatag tttatctcag
    16801 tctagtttct gatgttttaa gtagagtttc ttcgttagat gacacaactt tcgcattgat
    16861 tagctccgac ttcgctacta tcatctgtaa aagtacggat atagtagatt gatttcacac
    16921 ctttgttgaa agcatagtta cgtaggattg aaaggtcgcg agtagtctgc ttactttctg
    16981 ttttccactc gtacaactcc tttggtaatt cgcttcgtaa aaagagtgtc attgagagac
    17041 cttggtctac gtgctcagtt gctgcggcat agacgtctat tactttacgc atatccatat
    17101 cataggcaga agtgtaataa gggatagtat ctgtagctaa gccattagct ggataataaa
    17161 ttttcccgat ttttttctct tgacgctctt caatacgttg tgtaataggg tggatagatg
    17221 ctgaacaatc gttgatatac gaaatagaac cattcggagc aactgcaagc cgattttgat
    17281 gatatagtcc gtctttcata actgcgtatc taaggttttc ccaatcttta gcttcgggga
    17341 taaaatgatt agtaaatagg gattttactt tgtcagattg tggaacaaat tttccagata
    17401 catatttatc aaagtatgta ccgtcagcgt attttgattt ttcaaaacct acaaatgttg
    17461 tttgacgttc gcgggcaata ttgtttgatt ctactaaggt ccagtagttc attagcatga
    17521 aataaatatc agtaaattca attgattctg gactaccata ttcgatatga ttttttgcca
    17581 aataggagtg gagtcccatt gctccaagtc caaatgtatg tgcttgagca tttccgttct
    17641 taatagttgg aacagcttca atatttgatg agtcagtaac gaaagttaga gcacgagtca
    17701 ttgttttaat tgattttcca aaatcaggag atgtcatcat atttaaaaca ttagttgagc
    17761 caaggttaca agaaatatca gttcccattt caaggtattc ttgagcatca ttgataagac
    17821 ttggtttttg tacttgcaaa atctcagagc aaagatttga cataataatt tttccgtcaa
    17881 caggattagt acgattagca gtgtcaatat tgatgatgta aggataacca gattcttgtt
    17941 gaagtttaga aatctctgtt tctaaatcac gagcattgat ttttgtttta gtgatattag
    18001 ggttagcgac taattcatca tatttttcgg taatatcaat atagctgaaa ggaacaccat
    18061 attcacgttc gatactataa ggactaaaca agtacatttc ttgattgtta cgtgctagtt
    18121 catagaattt atcgggtaca gtaatcccta atgacaatgt tttaacacgt actttttcat
    18181 cagcattttc ttttttagtt gataggaatg aaataatatc tggatggaaa acgtcaaggt
    18241 aaacaactcc agcaccttgg cgttgtccaa gttggttgga gtaagaaaaa ctgtcttcaa
    18301 ataatttcat gactggaacg actccagaag cagcaccagc aaaacctttg ataggagctc
    18361 cagcctcacg aaggttacta agtgatattc caaccccacc accaatacga gataattgaa
    18421 gagctgaatt aatcgaacga ccaatagcat tcatatcatc agttacttgg attaagaaac
    18481 atgagactaa ttctccgcga cgacttcgac cagcatttaa aaaacttggt gttgcaggtt
    18541 gatagcgttg tgaaatcatt tctagagcta agtcagttgc tagttcttca tcaccgtctg
    18601 caaaataaag agcgttaaag agaacgcgat cttcaatact ttctaaatag tattctccat
    18661 catttgtttt taatgcatac tgctgataaa acttataggc tgccatgaag gatttaaaac
    18721 gaaacttttt atcgaagagt ttggtagata attcttcaat aaattcagga cgatatttat
    18781 tgaggaaagc ttcttcaaga taatcatact taaggaggta agcaatttta tcagtgattg
    18841 agtcaaaagc ttttgaatta ggctgtacgt tttctttgaa aaaagcagtt aaggcttctt
    18901 tatctttatg gagtgggatt tgtccattaa caggacggtt aatttcatta tttaggcgaa
    18961 agtaagatac atcgccaata ttttttaaac tcattgaatt tatcctctta acttaacaat
    19021 acaaaatggg aataaagcaa ttaattgctt tattcccaga ctctaatttg cttaaacaag
    19081 ttcttttagt ttgcttggtt ggaaacctga aaaaacaaca ttaccagctt caatcactgg
    19141 agccgcagaa aagccgagat ttttcacata ttcaattttt tctggttttt catcaatatt
    19201 aatttcttca aaatcagcgc cgtgctggtc aagaaatttc ttagtcattt tacattgcat
    19261 acagttgttt ttcgaaaaaa cagtaatagc tgccatgttg tcctcctaat tttttttcat
    19321 ggtaatagca tataaaaaaa gaatggaaaa agcaagtgtt ttatttctaa caaaacacaa
    19381 tatatagtgt cttaaaataa aatgagacac tatatttcgt ttttagggtt agtttaaaag
    19441 ctttcagcct gttttccaat tcctttcaga agaacataaa aaaatcattt ttagaattct
    19501 atttttaaat tctaagactc tcatttttaa tatgaaaagc tgtggaaaac attcaagttt
    19561 ttttctgttt gagacattta atcttgaaaa ataaaaagca atatgttata cttttaactt
    19621 gtaagggtta ccaataggtg actcaaatat ttacaaatga caaaaggaga accataatat
    19681 ggcttcaaaa gatttccaca ttgttgcaga aacaggtatt cacgcacgtc cagctacttt
    19741 gcttgtccaa acagctagca aatttgcttc agacatcacg cttgattaca aaggtaaagc
    19801 agtaaacctt aaatcaatca tgggtgttat gagtcttggt gttggtcaag gtgctgatgt
    19861 tactatttca gcagaaggtg cagatgcaga tgatgcaatt gcagctatcg aagaaacaat
    19921 gacaaaagaa ggattggcat aagaaaatga cagaaatgct taaaggaatc gcagcctctg
    19981 atggtgttgc agttgctaaa gcatatctac ttgtccaacc ggatttgtca tttgagactg
    20041 taacagtcga agatacaaac gcagaagaag ctcgccttga tgttgcactc caagcttcac
    20101 aagacgagct ttctgttatc cgtgagaagg cagtagaaag cttaggcgaa gaagccgcag
    20161 ctgtatttga tgctcattta atggttcttt ctgacccaga aatgattaat caaattaaag
    20221 agactattcg tgcaaaacaa gtaaatgcag aaacaggtct taaagaagtg actgatatgt
    20281 tcatcactat ctttgaaggt atggaagata acccatacat gcaagaacgt gcagcagata
    20341 tccgtgatgt tgccaaacgt gtcttagcac atcttttggg tgttaaatta ccaaacccgg
    20401 caactatcaa tgaagaatca atcgttattg cacacgattt gacaccttct gatacagctc
    20461 aattaaataa acaatttgtt aaagcttttg taacaaatat tggagggcgt acaagtcact
    20521 cagctattat ggcacgtaca ctcgaaatcg cagctgttct tggtacaaat gatattactg
    20581 aacgtgttca agatggtcag ttgattgctg ttaatggtat tactggtgaa gttatcattg
    20641 aaccaactga agctcaaatt tcagccttca aggcagcagg tgaagcttat gccaagcaaa
    20701 aagctgaatg ggcacttctt aaggatgcac aaacagttac tgctgatggt aaacattttg
    20761 aattagctgc taatatcggt acacctaaag atgttgaggg tgttaacgaa aatggagcag
    20821 aagcagttgg tctttaccgt acagagttct tatatatgga ttcacaagat ttcccaacag
    20881 aagatgagca atatgaagca tataaagcag ttcttgaggg tatgaatggt aaaccagttg
    20941 ttgttcgtac aatggatatc ggtggagata aagagcttcc ttactttgat cttccaaaag
    21001 agatgaaccc attcttagga ttccgtgcat tgcgtatttc aatttctgaa actggtgatg
    21061 caatgttccg cacacaaatc cgagcacttc ttcgtgcttc agttcatgga cagctacgca
    21121 tcatgttccc aatggttgct ttgcttaaag aattccgtgc tgctaaagct atatttgaag
    21181 aagagaaagc gaacttgtta gctgacggtg tagcagtagc tgaaggcatt gaagttggta
    21241 ttatgattga aatcccagcg gcagctatgc ttgcagacca atttgcaaaa gaagttgatt
    21301 tcttctcaat tggtactaat gatcttatcc aatacacaat ggctgcggac cgtatgaatg
    21361 agcaagtttc atacctctat caaccataca atccatcaat ccttcgtttg attaacaacg
    21421 ttatcaaagc tgctcatgct gaaggtaaat gggcaggtat gtgtggtgag atggccggtg
    21481 atcaaactgc ggttccactt cttgttggta tgggattgga tgaattctca atgtcagcaa
    21541 cctcagtact tcgtacacgt agcttaatga aaaaattgga cacagctaaa atggaagaat
    21601 acgctaatcg tgctctttca gagtgttcaa ctatggaaga agttatcgaa cttcaaaaag
    21661 aatacgttga ctttgactga gaataataaa aaagtaggga ttaaaaaatc tctacttttt
    21721 tattgaaagt ttggatattg ttaagtttct aagaaaaata tgtcagataa ttgaaataaa
    21781 tagataaagt gatagaataa acctattaca atgtaaaagg agatttactt tgacaaaaga
    21841 atatcaaaat tatgtcaatg gcgaatggaa atcatctgtt aatcagattg agattttgtc
    21901 accaattgat gattcttcat tgggattcgt gccagcgatg actcgagaag aagttgatca
    21961 tgctatgaaa gcgggtcgtg aggctttacc agcttgggct gctttaacag tatatgaacg
    22021 tgcacaatac cttcataaag ccgcagacat tattgaacgt gataaagaag aaattgctac
    22081 tgttttagca aaagaaattt ctaaagctta caatgcttca gtaactgagg ttgtaaggac
    22141 agctgatctt attcgttatg cagcagaaga aggaattcgt ttatcaactt cagctgacga
    22201 aggtggaaaa atggatgctt caacaggtca taagttggct gttattcgtc gtcaaccagt
    22261 aggtatcgtt ttagcaatcg caccttataa ttaccctgtt aacctctcag gatcaaaaat
    22321 tgcgccagct ctaattggtg gaaacgttgt gatgtttaaa ccaccaacac aaggttcagt
    22381 ctcaggactt gttttagcaa aagcttttgc agaagcaggt cttccagcag gtgtctttaa
    22441 tactattaca ggacgcggtt ctgagattgg agattacatt gttgagcatg aagaagttaa
    22501 ttttattaac tttacaggat caacgccagt tggacaacgt attggtaagt tggcaggaat
    22561 gcgtccaatt atgcttgagt tgggcggtaa ggatgcaggt atcgtcttag ctgatgctga
    22621 ccttgataac gctgctaaac aaatcgttgc aggtgcttat gattactctg gacaacgctg
    22681 tacggcaatt aagcgtgtgc ttgtcgttga agaagttgca gatgaattgg cagaaaaaat
    22741 atctgaaaat gtagcaaaat tatcagtagg tgatccattt gataatgcaa cggtgacacc
    22801 ggttattgat gataattcag ctgactttat tgaaagctta gtagtagatg cacgtcaaaa
    22861 aggtgcgaaa gaattgaatg aatttaaacg tgatggtcgt ctattaactc caggattgtt
    22921 tgatcatgtt actttagata tgaaactagc ttgggaagag ccttttggac caattctccc
    22981 aattattcgt gtcaaggatg cagaagaagc tgttgctatt gccaacaaat ctgattttgg
    23041 attacaatca tcagtcttta cacgtgattt ccaaaaagca tttgatatag caaataaact
    23101 tgaagttggt acagttcaca ttaacaataa gactggacgt ggtcctgata acttcccatt
    23161 cttaggactc aaaggatctg gtgcaggtgt tcaaggtatc agatattcaa ttgaagcaat
    23221 gacaaatgta aaatcgattg tttttgatat gaaatagatg taaaacgact caacgattgt
    23281 cagttgagtc gttttataaa cctcttttac attaaaaata aagaattata taatcttcta
    23341 aagtggtttt atttttgtga tataat
//
LOCUS       DATRME010000026        23265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRME010000026 DATRME010000000
VERSION     DATRME010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            149..1483
                     /locus_tag="VBC23_001009"
     CDS             149..1483
                     /locus_tag="VBC23_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6842966.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1551..2339
                     /gene="pflA"
                     /locus_tag="VBC23_001010"
     CDS             1551..2339
                     /gene="pflA"
                     /locus_tag="VBC23_001010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6842967.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2456..3391
                     /locus_tag="VBC23_001011"
     CDS             2456..3391
                     /locus_tag="VBC23_001011"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6842968.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3451..4104
                     /locus_tag="VBC23_001012"
     CDS             3451..4104
                     /locus_tag="VBC23_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6842969.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4134..5135)
                     /locus_tag="VBC23_001013"
     CDS             complement(4134..5135)
                     /locus_tag="VBC23_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6842970.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5132..6157)
                     /locus_tag="VBC23_001014"
     CDS             complement(5132..6157)
                     /locus_tag="VBC23_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6842971.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6173..7105)
                     /locus_tag="VBC23_001015"
     CDS             complement(6173..7105)
                     /locus_tag="VBC23_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6842972.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7129..7923)
                     /locus_tag="VBC23_001016"
     CDS             complement(7129..7923)
                     /locus_tag="VBC23_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842973.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(8082..9533)
                     /locus_tag="VBC23_001017"
     CDS             complement(8082..9533)
                     /locus_tag="VBC23_001017"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6842974.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            9657..11291
                     /locus_tag="VBC23_001018"
     CDS             9657..11291
                     /locus_tag="VBC23_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6842975.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            11426..12646
                     /gene="pepT"
                     /locus_tag="VBC23_001019"
     CDS             11426..12646
                     /gene="pepT"
                     /locus_tag="VBC23_001019"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6842976.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            12675..13166
                     /locus_tag="VBC23_001020"
     CDS             12675..13166
                     /locus_tag="VBC23_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6842977.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(13153..13350)
                     /locus_tag="VBC23_001021"
     CDS             complement(13153..13350)
                     /locus_tag="VBC23_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6842978.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            13396..13920
                     /locus_tag="VBC23_001022"
     CDS             13396..13920
                     /locus_tag="VBC23_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6842979.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            13931..14614
                     /locus_tag="VBC23_001023"
     CDS             13931..14614
                     /locus_tag="VBC23_001023"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6842980.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    14635..14760
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14775..15305
                     /gene="infC"
                     /locus_tag="VBC23_001024"
     CDS             14775..15305
                     /gene="infC"
                     /locus_tag="VBC23_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6842981.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            15345..15545
                     /gene="rpmI"
                     /locus_tag="VBC23_001025"
     CDS             15345..15545
                     /gene="rpmI"
                     /locus_tag="VBC23_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6842982.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            15603..15962
                     /gene="rplT"
                     /locus_tag="VBC23_001026"
     CDS             15603..15962
                     /gene="rplT"
                     /locus_tag="VBC23_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6842983.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(16205..18349)
                     /locus_tag="VBC23_001027"
     CDS             complement(16205..18349)
                     /locus_tag="VBC23_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6842984.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            18484..19641
                     /locus_tag="VBC23_001028"
     CDS             18484..19641
                     /locus_tag="VBC23_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6842985.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHVYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            19641..20318
                     /locus_tag="VBC23_001029"
     CDS             19641..20318
                     /locus_tag="VBC23_001029"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6842986.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            20412..21479
                     /locus_tag="VBC23_001030"
     CDS             20412..21479
                     /locus_tag="VBC23_001030"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6842987.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            21480..22646
                     /gene="aroC"
                     /locus_tag="VBC23_001031"
     CDS             21480..22646
                     /gene="aroC"
                     /locus_tag="VBC23_001031"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6842988.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            22730..23065
                     /locus_tag="VBC23_001032"
     CDS             22730..23065
                     /locus_tag="VBC23_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6842989.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         7598 a         3660 c         4459 g         7548 t
ORIGIN      
        1 tgctataaaa tgccataaac aatattatcg actatgcgct cagtgctatt atatgatata
       61 ataatagcat tatctaatag aagaacagtt ggtagagtta atcgctatca gctaaattca
      121 aataattttt atttaggagt aattttccat ggaagaccct ggcagtcaga gtttgttact
      181 acaatttgtt attttattaa tattaaccct gtttaacgcc ttcttttcag catctgaaat
      241 ggctttagta tcgctaaacc gctctaaagt agaacagaaa gctgaagaag gggacaaaag
      301 gtatcgacgt cttttggacg ttcttgaaaa tcccaataat tttttatcaa caattcaagt
      361 tggtattact tttattagcc ttttgcaagg ggctagttta tctgcctcac taggacatgt
      421 gatttcagga tggctaggaa attcagcaac agcccgtaca gcagggagta ttattgcgct
      481 aatcttttta acatatgtct ctattgttct aggagaatta tatcctaagc gtattgctat
      541 gaatttgaaa gatcgtctag cgattgtttc agctcccatt attatctttc tggggaaaat
      601 agtaagcccc tttgtatggt tgctctcagc ttcaactaac ttattgagta gaatcactcc
      661 gatgactttt gatgatgctg acgagaagat gacacgtgat gaaattgaat atatgttgac
      721 gaatagtgaa gaaactttgg atgctgaaga aattgagatg ctgcaaggga ttttctcgct
      781 agatgaaatg atggcgcgtg aagttatggt tccgcgtact gatgctttca tgattgacat
      841 caacaatgat gcacaatcaa atattgaagg aattttatca caaaactttt ctcgtgtccc
      901 tgtttatgat gacgataaag atagagttgt tggtgtttta cataccaagc gcttattgga
      961 agcgggcttt aaaactggtt ttgatactat cgacttgcga aaaatacttc aggaacctct
     1021 ttttgttcca gaaacgattt ttgtagatga tcttttaaaa gcattacgca acactcaaaa
     1081 tcaaatggcc attttactag atgaatatgg tggcgttgct ggtttagtaa ctctagagga
     1141 tttattggaa gaaattgtcg gcgaaattga tgatgaaaca gatacagctg aacaatttgt
     1201 cagggaaatc gatgagaata tctatattgt tctaggaact atgacgctta atgaatttaa
     1261 tgattatttt gagacagagt tagaaagtga tgatgttgat acaattgcgg gttattattt
     1321 aactggtgtg ggtagtattc caaaccaaga ggaaaaagta gcttacgaag tagacagcaa
     1381 agataaacgc atcactttga ttaatgataa agttaaagat ggtcgtataa caaaattgaa
     1441 agtgctattg tctgatatag aacagaatat tgaaaaagac taagattact tagtcttttt
     1501 ttcatgaaaa tgatataata aagtcataaa aacggttaca aggtgatgaa atggcagaaa
     1561 tagactataa aaaagtaaca ggaatgatcc attctacaga aagtttcggc tctgtggatg
     1621 ggccaggtat tcgttttatc atttttatgc aaggatgtaa aatgcgttgt caatactgtc
     1681 ataatcctga tacttgggaa atggaaacta acaattcaaa ggaacgcacc gttgaggatg
     1741 ttctaaaaga agctcttcgt tataaacatt tttggggaaa agatggaggt atcacagttt
     1801 caggtggtga agcaatgctg cgaattgact ttattacagc tctctttatt gaggctaaga
     1861 aactaggtat tcatacgacg ctagatactt gtggttttgc ttatcgagca acaccagaat
     1921 accatgctat ccttgaaaaa ttgttagatg ttactgattt ggtactcctt gacttaaaag
     1981 aaattgatcc agaacaacat aaaattgtaa ctcgtcagtc taacaaaaat attttacagt
     2041 ttgctagata tttatctgat agaggtactc cggtatggat tcgtcatgtt ttagttcctg
     2101 gattgactga tattgatgac catttaaaac gtttgggtga atttgtccaa acacttgata
     2161 atgtcgataa atttgaagta ttgccttatc atactatggg agagttcaaa tggcgtgaat
     2221 taggaattcc gtatccatta gctggtgtga aaccaccaac tccagaacgt gttaaaaacg
     2281 ctaaagatat catgaaaaca gaatcctata cagaatatct aaaaagaatt caaaattagt
     2341 tttaagttga attttattca acttttttag ttgcaaaggg cttcctagta agattttttg
     2401 ctaagttatt caatttttgc tacaataagg tgaatataaa agaagaggta aaaacatgtc
     2461 taaaatttta gtctttggtc accaaaatcc agattcagat gctattggat catcagttgc
     2521 ttttgcatat ctagctaaag aagcttgggg gcttgacaca gaggccgttg ctttaggaac
     2581 tccaaatgaa gaaacagcat atgttttaga ttattttggt gttcaagcac ctcgagtagt
     2641 agaatctgca aaagcagagg gtgtagagac agtcatttta actgaccata acgaattcca
     2701 acaatctatt tctgatatta aagatgtgac agtttatggt gttgttgatc atcatcgtgt
     2761 tgctaacttt gagactgcca atccgttata tatgcgtttg gaaccagttg gctcagcatc
     2821 atcgattgtt taccgtatgt ttaaggaaaa tggagtatca gtacccaaag agttggctgg
     2881 tttattatta tcaggtttga tttcagatac tcttcttttg aaatcaccaa caactcatgc
     2941 ttcagatatt ccagttgcaa aagaactagc agagcttgca ggtgtaaact tagaagaata
     3001 tggtttagaa atgcttaaag caggtactaa cctctcaagt aagactgcag cggagttaat
     3061 tgatattgat gctaaaacat ttgaattaaa tggagaagct gttcgtgttg cacaagttaa
     3121 tactgtggac attaacgata ttttagcacg tcaagaagag attgaagttg ctattcaaga
     3181 agcaattgtc acagaaggtt attcggattt tgtattgatg attactgaca ttgttaattc
     3241 aaattcagag attttagctc tcggttcaaa tatggctaaa gttgaagcag cttttgaatt
     3301 tacacttgaa aataaccacg cttttcttgc tggggcagta tcacgtaaaa aacaagttgt
     3361 tcctcaattg acagaaagct acaatgctta attaatcatg taaaacgggc tgcgtcccgt
     3421 ttttttagtc atacatttag gagcgttttt atgatattaa cgtttaatcc aggaaagtta
     3481 gaaagacaag agttttttaa ggagcttatt aactatcttt ggatacatga tgatgtaaca
     3541 ctaagacaaa taaagtccca ctttacagac tattctaaaa ttgaccgttt gttagaagaa
     3601 tatattaatc atggatatat ccttcgccaa aataaacgct acagccttaa tcttcctttt
     3661 ctgagttcgc tggatggatt agcattagat gatttggttt tcatagattc tgctagtcaa
     3721 atctatcagt tattacagaa gcgaaaattt gtgacaaatt tagataacca aaccaatcac
     3781 ttggtatttg tagaagagac agattttgaa agaaacactt tgactctttc taattatttc
     3841 tataaactaa ctaactgtta tccactttca agagaacaaa aaaagcttta tcaactttta
     3901 ggagatgtta attcggaata tgctttaaaa tatatgtcaa gctttatatt gaaatttctt
     3961 cgcaaggata gtgttaagca aaaacgtaca gatattttta tccaagcttt agaattactt
     4021 ggatatatta gcttaaacca agatactacc tatcgtttaa atgctaaact tgatgttgaa
     4081 gctttaaaga tttacttaac ataaaaaaag actggaagtt tgtccagtct tttttagtca
     4141 acttttgtca tcaaccagag aaaatagggg gcaccaatta ttgatacaac caatccagtg
     4201 ggtatacctg taccaactaa gaggagacga cctatagtat ctgataccaa taggataata
     4261 gcaccaataa gaagacagct aggtagtata attttatggt tatttcctaa tagtcgtcta
     4321 cttaagtgcc cagcgagtaa gcctataaaa gtaatattac caattaagac gacgctaata
     4381 gcagcaaggc tacttgccag catcaaagta tatattcttt ccttttttaa atgaagccct
     4441 agagcgagag cagtctgctc gtttaggttc ataatattaa ggtggcgact gcgactatat
     4501 gctaatcccc ataacaaaat gagaatcggg ataaaagtga taatggtagt ccaggaacta
     4561 attgtaattt ttccactaag ccattcaacg atcatatcca ttttttgatt attagactga
     4621 ctaataatag aaatcataac gcctgaaatc attgtagata ttccgatacc agtgatgatt
     4681 aatcgtgtag ggctaatatt gtgatttctt ctgtatgaca taagatagac aagaaaaata
     4741 gttaccaacc ctccaaacat agcaaatagg ggtaaaaagt ataatatagt tggattgcta
     4801 acattaaatg ttcctatggt tagagcgatt actaatccag ctccagcatt gataccaaga
     4861 ataccggaat cagctagtgg atttttggtt aatgtctgta atagcaaccc agcaattcct
     4921 aaagaagctc ccccaaagat acagacaata atacgaggta aacgaatgtt agtaatgata
     4981 aaaaggaagg catcatcaga ttttccactt attagtttta aaacgtcaat aacggaagta
     5041 ttagcatagc caagtgaaat tgatactagt attaataata gtaggataat gacagatgat
     5101 attaaaacaa aaggagcttt atttttttga atcatagatt tttccctttt ctaattaacc
     5161 ataggaaaca tggaagccct acgatactga ttatagaact aattggtgtc tctgaagggg
     5221 gatttataat acgagatgat aagtctaccc agatcatgaa agtagcccct gaaaatccta
     5281 ttaaaggtaa taacagacga taatctttgg gaataaaaag cttaataaaa tgtggaataa
     5341 tcaatccaat gaaagaaacg gttcctatca acgcgactga tgaagccgat aaaaacaaaa
     5401 caataagtaa tagaaacgca gtcattaatt gagttttttg tcccaatgct ttggccacag
     5461 attcattaag gctcaagata gttaattgat gtgctaggag ttgtgaaata agtaaaccta
     5521 aaataatgat aggtgctatt atgattagca ttttccagtt aatttgggat aaaccacctg
     5581 cttgccaacc gataacagtt cgattgagtt taaagtataa tgtaaccact tgtccaacag
     5641 acgtaaaaag tgtggagatc atcgctccag ctaaaatgag tcgtagttgg tggtaaccct
     5701 tttgctttgt ataagagagt gtgaagacta aaagacaaga aatgacagac cctagtaagc
     5761 agacaatgag aatcgtacta tagtgcatgc tccctaaaaa agcatatgca acaacaaggg
     5821 ctaacccagc tccggcgtta atacctagta gtccaggatc agcaatggcg tttcgagtga
     5881 ctccctgcat aatagcacct gcttgtgaca tagctgcacc tactaagata gctgcaatta
     5941 tgcgtggtag tctaatgtca aaaataacat cctgagcatt agagtttttt aagggatttt
     6001 ttaaaacagt tattaaatca ctagtcttga aattgatagc accaaatcgt aagctggcgt
     6061 aacaaccaat aagaaatagt ataagtgtta tgaaaaaaac aagccaaaaa tgatttgact
     6121 tatttttttg aacgagtttt tttgaagctt taaacataag aggagctttc ttttaatttg
     6181 tattttcttt gatagccttt gtaaatgatt ttaattgagc ttctaaagat agagggtcag
     6241 agaaataaaa cacgtcgtag ttactttcta tgatgtgccc ttttttgaca gctggtaaat
     6301 tcttccagac atcactttct ttaagtgatg aagctgcttt tttagtcgtt ttgtttatat
     6361 taacaagggc ataatctcca acgtaatcac cgattgcttc ttgcgaaacg gtaaaccacc
     6421 cttttttaaa gacatctttt ttgacttttt ctggggcagc ataacctagt gaatcataga
     6481 ttagttctcc accgcgtcca aaattattac catataaata gatattttta tcataaaaat
     6541 ccataatagt aaaagtagtg ttaggcttta agatatggtg taaatctttt ttggcagcga
     6601 gagttttagt tttccattgg ctaacccact gattagcttc tttttcttta ccgaatactt
     6661 tccccaaggc tggcatcata tctaaataat tttgtgcacc atatttaata actaaagttg
     6721 gtgcaatttt tttcagagta ttgatgtttg gatcttgatc gaaaaccatg attaaatcag
     6781 gtttttgtgc ggcaatagct tctgtatcat cagcagttaa ttttttagct tctttcagtt
     6841 gcttaccaaa aacggggcta tctttttcta agtctaaact gtaacttgaa acattaacac
     6901 ctagttttaa taaataccca gtgtaagaat atgtaaaatt aattactttt ttcggatttt
     6961 caggaatttt tccataatag gtgaagcctt caattttagg cattttagaa atagttgttt
     7021 tagtgctttc ttctttagtt tgttgtctgc aagataccaa aaagaaggtc agtagtgtga
     7081 ggacaataat tccaattttt ttcataagat aacatctcct aaataatatt aattttgatc
     7141 agttgataat tgataagata acaaaacagg ttgtttacta atagggtcct gaatgatatg
     7201 agcatcaatc ttaaaaatat ctttgataat atctttagtc atgatctgac taggactacc
     7261 ttcatagata attttaccgg ttttcattgc aactagataa tcagaatagc gcgcagacag
     7321 gtttaggtcg tgtaggacca taataattgt tttttgcgtt tcgttattaa gttttttgag
     7381 caattctaag acttctaatt gatgattcat gtcaagataa gttgtaggtt cgtctaaaaa
     7441 gatagtatct gtatcttgtg ctaaagccat agcaatccat actttttgcc tctgacctcc
     7501 tgaaaggtca tcaacatctc tattagcaaa ttgcgctacg ttagtcattt ccatcgccca
     7561 atggatttta ctacgatcat catttgtcaa attgccaagg tatttttggt gtgggaaccg
     7621 tccataagat attagttcat aaactgttat cccttcagtt gcttctaaca cctgtgggag
     7681 aagtgcaatc ttcttagcaa tttcctttgt gggtaaatgt gcaatatcat gaccatcaat
     7741 agtgatagta ccatctttta ttttatggat ccgtgtgaga gccttcaaaa gagtagactt
     7801 tccacatcca ttggcaccaa taatcgttgt tattttttgg ttaagtatac tgagggaaag
     7861 attattgatg atttctttct gatcgtaaga tacaataatg ttttctgctt ttatgtgaga
     7921 catagagaac ctttctagca aaaaattaag ttcattataa catagaaaaa aggcttacaa
     7981 aagccttttt tagcattaat taagaaaaat gtgaacgaaa tgataattat ttagaatgat
     8041 tcaaaatagt atcaataatc attattgttt agcttttttt atcatttaag gtattttttc
     8101 gcaacttcta aatctccaga gtaattgtca cgatttcctt tgataatttg ataagcatct
     8161 gcccctttac cagcgataat aattgcatct agtttgcaat tagtaagagt actggcatta
     8221 gcaattgctt cttctcgatc aatgatgata gtaaccggac gattaatatg gcttgcgatt
     8281 tcttgtgaga taactaaggg atcttcaaaa ttaggatcat ctgcagtgag tatcacttgt
     8341 agattaggat gttgattaat gacatctccg aagtctttac gacggctttg acctttatta
     8401 ccaggagctc cgagcactaa tataatatcc cctttttggt gttcctcaac aacagctaat
     8461 aatttcttca aactgtctcc gttgtgggca tagtcaacga aaatctttgc gccgttggtt
     8521 tgtgttagga cttccatgcg gccagggacg gtagtttgtg caattccgtt ttttatatct
     8581 tcaattgaaa caccaagtcg gagacaagca agtccagctg cgagagcatt ttcttgattg
     8641 aattttccaa tcaggttgat attgtaggtg ttctccaact ttcctttgac atggaaagaa
     8701 aaggcctgtg attctgtgat aacattttca gaataatctc catagaaatc atgaggaata
     8761 tattctacct gttctttgac aatattaaaa tgatccatct gactatttac aacgacagca
     8821 ttgctattct ccatcaagag ccgtttgtgg aaaaaataat cttcgaatgt agggtgctcg
     8881 ataggaccaa tatgatcggg actaatattt aagaaaacgc caacgtcaaa ggttaaacca
     8941 taaacacgtt tggttaggta tgcttggctt gatacttcca tgactaagtg tgtcatttga
     9001 ttctcaacgg ctgtagccat catccgaaat aaatcaagac tctcgggggt tgtgagatgt
     9061 gatttaaaga aagatttacc atctaatgtt gtgttcatag ttgagagcat agcaggacga
     9121 tggtttactt ttagcatatg ataagcaaag taagccgcag ttgtttttcc ttttgttcca
     9181 gtaaaagcta gaagtttaag tttgttttgt gggttattat agaaagacat agatatcaaa
     9241 ctcatggctt ttttgatatc attgactaat atcactggaa tatcggcgcc gtaatctatc
     9301 tcagaaacgt aaaaagaaag gcctgctgtg attgcagaat ctagatattc ttttttaaaa
     9361 gtggcaccct tagcaaaaaa taaagtatca gatgaaattt gacgactatc atatgataag
     9421 gcattaaaag ttacattttg tgtccagtta taataataat gctcatgaaa caaaatctct
     9481 ctgaaattat ggtcattttt taaaatttct aaaattttat ctattgtaat catactcctt
     9541 attttagact ttatgttctc gttttacaag tctaaggtaa aagtttataa taaagtgata
     9601 tttctgagat ttttgtcttt taaatgctaa ataatattga aaatagaaag tttaccatgt
     9661 cacaaaaaac aacaaaagtt agtcagcaag aacaaatggt caaagggacg gcttggttaa
     9721 cagcagggaa tttcattagt cgcttgctgg gagccatcta tattatccct tggtatgttt
     9781 ggatgggaaa gcatgctgcc gaagcaaatg ctttatttgg gatgggatat gaaatctatg
     9841 ctttatttct tttgatatca actgttggaa ttccagtagc agttgcaaaa caagtatcta
     9901 agtataacac tttaggtaaa gaagagatga gcatttatct ggttcggaaa atcttgcagt
     9961 ttatgctgat tttaggtgga gtttttgcat taattatgta tattggttca cctctatttg
    10021 cttcacttag taaaggaggg caagaactcg ttccaatatt gcgtagtcta actctagctg
    10081 ttttagtttt tccttcaatg agtgttttac gtggtttttt tcaaggtttt aataatttaa
    10141 aaccgtatgc cattagtcaa gtcgctgagc agattatacg tgtcatatgg atgttattaa
    10201 ctgctttcta tattatgcgt ctaggatcag gagattatat tgctgctgtg acacagtcaa
    10261 cattcgccgc ctttgtcggt atgtttgcta gtatagctgt gctgctttat ttcttatggc
    10321 gttataatat gctatctgcc ttgattagta aaactccaga gcacattaaa ttagatacta
    10381 aagaaatcct aattgagact gttaaggaag caatcccttt tattattact ggtgcagcaa
    10441 ttcaaatttt caaattgatt gatcagtttt catttgggaa tacgatggcg ctctttacga
    10501 attattcaag tgaagaatta cgggttatgt ttgcttactt ttcaagcaac cctggtaaag
    10561 ttacgatgat tctaattgct gttgctaccg caattgctgg agttggtatt ccattgttaa
    10621 cagagaattt tgttaagaat gataaaaaag ccgccgctcg tttggttgtt aataatttac
    10681 aaatgttact aatgttttta ctgccagctg ttgcaggatc cgttatttta gcaaaacctt
    10741 tgtatacagt cttttatggg ttaccacagg gacaagcttt agggcttttt gtcatatctc
    10801 ttattcaaac aattatttta tctatttata ctgttttagc accaatgcta caagcattgt
    10861 ttgaaaatcg taaagctatt atttacttcc tatatggtct ggtggcaaaa gttattttac
    10921 aagttccctc cattttcctt ttccatgcct atggaccact tttctcaacg acagtagctc
    10981 tttgtatccc agtaattctt atgtatttga aaatacatga aattacagga tttaaacgcc
    11041 aagctattcg tcgtacaagt gctttagtcc ttattctaac gctattgatg agctttatta
    11101 tttctatgat aatatggtta atgaacctgg ttattgtacc cgatagtaga ctggtaagtc
    11161 tagtttacat tatcgttatc ggtgcaatag gtttaggggt atatggcttt atggcactgg
    11221 ccacccatct acttgataag atgattggta gtcgtgctca agacttacgc cgaaagttgc
    11281 accttaacta atgaaaaaat aggttagaca gaattgttta acctagtttt ttgacgacca
    11341 aaattatttg tgaaatttta aggtagtcac aatatagtga gcttattttt atgttataat
    11401 taaatggaga aatatgaggt gaaagatgtc ttacgaaaag cttttagaac gatttttaac
    11461 atacgtcaaa ataaatacaa gaagtaatcc taatagtaca caaacgccaa caactcaaag
    11521 tcaagttgac tttgctttaa cagttttaaa accagaaatg gaagcaattg gtttaaaaga
    11581 tgttcattat ttaccttcta atgggtattt ggttggaacc ttacctgcta caagcgaccg
    11641 cttacgccat aaaataggtt ttatatccca tatggataca gctgatttca atgctgaaaa
    11701 tattactcca caaattgttg actataaagg tggagatatt gaacttggag actcaggtta
    11761 cattttaagt ccgaaagatt ttccaaattt aaataattac catgggcaaa cattgattac
    11821 aacagatggt aaaaccttac tgggagcaga cgataagtct ggtatagcag aaatcatgac
    11881 agctatggaa tatttggctt cgcatccaga aattgagcac tgtgaaatta gagttggctt
    11941 tggaccagat gaagaaattg gtataggtgc agataaattt gatgttaaag attttgatgt
    12001 tgattttgcc tatacagtgg atggtggacc actaggagaa ttacagtatg aaacctttag
    12061 tgcagctggt ttggagctta catttgaagg acgaaacgtt caccctggaa ctgcaaaaaa
    12121 tcaaatgatt aatgctttac agcttgctat agattttcat agtcaattac cagaaaatga
    12181 acgtcctgaa caaacagatg gctatcaagg attttatcac ttatatgatt taagtggaac
    12241 agttgatcaa gctaaaagtt catatatcat tcgagatttt gaggaagtta atttcttaaa
    12301 acgtaagcaa ttggctcaag ctatcgctga taatatgaat gaagcattac aatctgaacg
    12361 tgtaaaggtt aaactatacg atcaatatta caacatgaag aaagttattg aaaaagacat
    12421 gacacctatc aacattgcta aagaagtaat ggaagagtta gacatcaagc cgatcataga
    12481 accgattcgt ggtggtacag atggctctaa aatttccttt atgggaatcc ctactcctaa
    12541 tctttttgca ggtggtgaaa acatgcatgg acgctttgaa ttcgttagtc tacaaacaat
    12601 ggaaaaagca gttgatgtta ttttaggcat cgttgctaag gattagtaaa gaaatatcag
    12661 attggagtgg ttaaatgata aaattatttg ggaagatacg ttatcactgg caacctgaac
    12721 tttcttgggc tattatttat tggtcaatag ccattgcacc aatttttatt ggtttatctc
    12781 tgctatatga aagaactgag attccgagtc aggtatttgt tctatttgcc atctttattg
    12841 ttttagtagg cattggattt catcgctatt ttgttattga agaagatgga tatcttagga
    12901 ttgtatcttt taacttttta agacgatcta agtttccaat tgaagatatt gctaaaattg
    12961 aggtcactaa atcaagtgtc actatcaaat ttaataataa ccatgagcgt attttttaca
    13021 tgcgaaaatg gcctaaaaaa tattttttag atgcactagc tattgaacct aaatttaagg
    13081 gagaagtgga gcttctcgat aatttgatta aaatggatta ttttgaatgt tatcgctacg
    13141 ataaaaaagc tcttacaaaa tcgtaagagc tttggtggga catgatttaa ctgctaaaac
    13201 agtatcttga tcagaacttg gaatttcttt ttctaggtta tctgtatcgg aaaatttaac
    13261 gataccatca tcttgataat caaaaatatt tgaatatgtt tggcaaagcc cgcaggcaat
    13321 acatttttct ggaattagtg caactttcat agtattattg taatacttat tgatacgatt
    13381 aacaaggggg aagtcatggc gaaaaaacca tgggaaaaaa aagttgtgga aaacaatagt
    13441 catagaaaag acaaaattac aagaacgagt agaggtgtgg tgtcaagtac tccttggatt
    13501 accgctttct tgagtgcatt ctttgttatt gtcgttgcta ttttatttat agtattttat
    13561 acttcaaata gaggagaaga cagagcaaaa gaaacttctg gtttttatgg tgcttcaagt
    13621 caaaaagtta atagttctaa aacgaaaaaa gcatctacaa gtaagaaaac aacgtcatca
    13681 tcaaaaacta aaactagtga taaatcaaca acatcttcta gtacagtagc aagttcgagt
    13741 gagacatcga catcatcttc agtaaacggt ggaacaatcg tagttcaacc aggtgaggga
    13801 gcagcctcca tagctgctcg tgcaggtatc tcagttgctc gtttacaaga acttaatcct
    13861 ggtcatatga cctcagggta ttggtatgct aatccaggcg atcctgtggt tgtaaattaa
    13921 ggggaggatt atgaactcaa ttaatattgc tattgatgga ccagcatcaa gtgggaaaag
    13981 tactgtcgct aagattattg ctaaaaattt gaattacact taccttgata caggtgctat
    14041 gtatcgttgt gctacttatc tggctttaca gcatggatat gaagcacaag atgtttcgaa
    14101 aattttagga ctcctagctg aaagaccgat ttcttttggt aaagcagaag atggcagcca
    14161 gacagtcttt cttggcacag aagaagttac tttagctatc cgtcaaaacg atgttactaa
    14221 caatgtctcg tgggtttctg ctatcccaga gattcgagaa gagttagtaa accagcagcg
    14281 tcgtatagcc aaagatggtg ctattataat ggatggtcgt gacataggaa cagttgtttt
    14341 gccagatgca gaattgaaga tttttttagt ggcttctgtt gatgaaagag ctgagcgtcg
    14401 ctttaaagaa aatcaagaaa aaggcattga gagtgatttt gaaacactga aatcagaaat
    14461 tgctgctcgt gattataaag atagccatcg agaagtttca ccattaaagg cagcagaaga
    14521 tgctattgaa tttgatacta caggagtatc aattgaaggt gttgtaactt ttattcaaga
    14581 aaaagcagaa aaaataattg acatgaagaa ttagtcatga tatactagtt gcaatgagaa
    14641 aagcagaagt gagaacttct cgccttgcga cttaggttgt ctggcccaac atgtcaagat
    14701 tccatctagg aagattatat gtgcgggctt gatttatcaa gtccgctttc gtttttctct
    14761 aaaaaaataa agaggtgaag atcatagcta aaaaagatct attcattaac gatgaaattc
    14821 gcgttcgtga agttcgtctg gttggtctcg agggtgaaca attaggtatt aaaccattgt
    14881 ctgaagctca agcaattgca gatgatgcta atgttgattt ggttttaatt cagccacaag
    14941 ctacaccacc tgttgcgaaa attatggact atggtaagtt caaatttgag tatcaaaaga
    15001 aacaaaaaga acaacgtaaa aaacaaagcg ttgttactgt taaggaagtt cgtcttagcc
    15061 cagttattga taaaggggat tttgaaacaa aacttcgtaa cggtcgtaag ttccttgaaa
    15121 aaggtaacaa agttaaagtt tctatccgat tcaaaggacg tatgattact cataaagaaa
    15181 ttggagcaaa ggtattggcg gaatttgctg aagcgacaca agatatcgct attattgagc
    15241 aaagagctaa gatggatggt cgccaaatgt ttatgcagct tgcaccaatt ccagacaaga
    15301 aataattgtc gactttatag tattatttaa gaggagaatt taaaatgcca aaacaaaaaa
    15361 cacaccgcgc atcagctaaa cgctttaaac gcacaggttc aggtggattg aaacgcttcc
    15421 acgcgtttac ttcacaccgt ttccacggta aaactaaaaa acaacgtcgt catcttcgta
    15481 aagctactat ggtttcatca ggagatttca aacgtattaa agcaatgctt actcgcctta
    15541 aataataagg tctctgtaaa catttactac atattataaa ggattatttg gaggaaatat
    15601 aaatggctcg tgttaaaggt ggagttgttt cacgtaaacg tcgtaaacgc gtacttaaat
    15661 tagctaaagg ttactatggt gcaaaacata tcttgttccg tactgcaaaa gaacaagtaa
    15721 tgaattctta ctactatgca taccgtgacc gtcgtcagaa aaaacgtgac ttccgtaaat
    15781 tatggatcac tcgtatcaat gcggctgctc gtatgaatgg tttatcatat tcacaattga
    15841 tgcacggttt gaaattagct gaaatcgaag ttaaccgtaa aatgcttgct gatttagcag
    15901 ttaacgatgc agcagctttc acagctcttg cagatgcagc taaagctaaa cttggtaaat
    15961 aagaaataaa aaagttgggt ctgcctaact ttttttaatt actagaaaga aagtacctat
    16021 attataattt aagtgaaaag tttcgttcta tatatgtaga atgaaacttt ttttgtttta
    16081 ttccgatacc tttattattg ttataatttt cctttttgat ttttattgtt ttatagtatt
    16141 attccgtata aaaagcacct caatcgttgg attgaggtgt ggattaaact ttaaatggta
    16201 aagattattt tttagatgtt ttatgtgtct tattaggatc ttgcaattcc aagtaacttg
    16261 gagcgtggaa gagatcaact gttgatttgt tgttatgttt gctgtatatg cttgttgaac
    16321 gatctttcaa tttacgttct ttagctttga gtgctttaag agaatgtgtg taattgaatt
    16381 tttcaacttc tacagttttc aaaccattgt tttcatcaaa tcgtagtaaa tctccagttt
    16441 gaatcgaatc acttgtagat aattgtttat tagtagcatc tcgaatagtt tttatttcag
    16501 ctttagttgt ttcatcagga tttgtgattt cttgtcctga atcagtgtaa tagagatgtc
    16561 cactatagtt agtgtattta ggagtaatat actgaccagt tgttctcata gcgaccattt
    16621 gtttattatc tttagataac aagtcttgtc ctaactgagt atatttagac gtgtctatcc
    16681 ctagaatatg aagtaaggtt ggtaaattat ctacctcacc accgtatgta ttactgataa
    16741 aaccttttga atagccagga atgtggatca tatatggaac acgttgtaac atagcgttgt
    16801 cgtattcaga ccatgtttca gggtctttac ctaataattc agctaagcta ggattgcgtg
    16861 tattagaaat accgtagtga tccccataca ttacaataat agaattatca tacacaccag
    16921 cagcttttag atattcaaaa aatgatttaa gagctgtatc aaggtagttt gctgtggcga
    16981 aataaccgtt aatcgtttca tcgttagtct ttgcaagtgg gaatccctct tcgtcagact
    17041 ctcccttcaa acttgtgtaa gggtaatggt tacttactgt aataaattta gtataaaacg
    17101 gttgttgcat gtgctctaga tatttgatag agtcagcaaa catatattta tcattcaaac
    17161 cgtattggaa tgaattatct ttggtttgtt ttgagaagta gctagagtca aagaagtagt
    17221 cataacccca ctgtttgtaa gcattatttc tattccaaaa tgttccgacg tttccgtgga
    17281 agaccgcact actgtaacct ccattttgag ctaagatatg tggagcagca aattgtgtat
    17341 tttcaccacc ataattaacc atgaatgaac cactacttag accaaaaagt gagttttcca
    17401 tgagtgtctc agcatcggat gtcttaccag ctttgacttg atggaagaag ttagagaagg
    17461 agatagtctc tttcgaatga taaaggctgt taataaatgg agtaaccaca tgttctttac
    17521 catcaacatt taatttatag tcaatcaaga attgttgaaa actctccaaa tgaatcatga
    17581 taacgtttcg cccttttccg ataccataat attctggatt gggtttggca taatgctctg
    17641 ctacatattt tttggcagtc gctaattctt gtgcagtggc accatttctt tctttctgtg
    17701 cttgataagt ttgattacca ctataaatcg aaaatgatgg caagccaaga gctttaacaa
    17761 tataggtgtt tgaaaaacca cgacttagta gttctggacg atcaatttca gctaagaata
    17821 agttaattga aaagagtaaa ccagacaaag ctgtgataga aaaactagca cgtttattaa
    17881 atggacgatt atctaagtgg attttcttgg ttgcaaataa gaaaataagg ataatgaaat
    17941 cgaaaacgta gacaagatcc caaattctca atagattaag agcagagtct ccaaggccag
    18001 ccgaagtttt actactagcc aagacagcgc taactgtaat gaaatcggaa aactctctat
    18061 agtagattgc gtttgcaatt agaaggatat ttacgatagc ataggtgata aaagcggtaa
    18121 tataaaaagc tttggttcgc ttaacatata aggccaaacc tatgattaag agtcctaagg
    18181 ggatggggtt tataatggtc aaaaacaact gataggaatt ttctaaacct aaattaaaat
    18241 cagtatggta agcccaaatt gtctttaacc aatatataac tactaacaat aaaataaagc
    18301 cgagtcgtgt attgatcgca cggctagcaa aatctttaat ttttttcaca gtaggaactt
    18361 cctttaattt ttttcctaaa gataaaatct cagattaaat tataacataa aattttgtaa
    18421 catacggtaa agaaaggtcg aattcagtat gaaaaaagat aaaaatttgt tataatactg
    18481 tttatgaata aacttaaagt gaattctgtc gttgaaagaa aaatcaaatc aggtgctcag
    18541 ttactggaaa aaaaagattt tgataccagt ttagttaacc agttggttca acttttttca
    18601 cagtcaaatc aattcttagg gatggcctat ctttcaccac aaaataaagg gattggttgg
    18661 ttactatcaa gacaaatttt tgattttaac catgtttact ttgtatcgct attcgaaaaa
    18721 tctagagaga agcgtcaaaa atttgaaaaa tctagccaaa caacagccta ccgcttgttt
    18781 aatcaggatg gagataactt tggtggccta actatagatt tttatagtga ctatgctctt
    18841 ttttcgtggt acaatgaatt tgtttatact aatcgacaaa tgattgttgc agcctttaag
    18901 caggtctatc ctaatattaa aggggcatat gaaaaaattc gtttcaaagg tttagacttt
    18961 gaaagtgctc atttgtacgg tcaagaggct cctgaatcat ttttgatttt agaaaataat
    19021 atcaaatata gtgtcttttt gaatgatggg ttaatgacag gtattttcct tgaccaacat
    19081 gatgtcagaa aagccttagc aactaatcta tcagaaggta aaaaagttct aaatatgttt
    19141 tcatacactg cggctttttc tgtagctgca gctgttggag gagcattaga gacaacttct
    19201 gttgatttag caaaacgctc gcgtgaactt tcaaaagcac actttgatgc taatcagatt
    19261 gtcacagata accatcgatt tatcgttatg gatgtttttg aatattataa gtatgccaaa
    19321 agaaaacatc tatcatatga tgtgattgtt attgatccac caagttttgc tcgaaataaa
    19381 aaacaaactt tttcggttac taaagattac tataaattaa ttgaacaagc tttagatatt
    19441 ttagcccctg gtggaactat cattgcatca actaacgcag ctaatctaac cgtatcacaa
    19501 tttaagaaac aattggaaaa ggggtttggt aaagcttcac ataattacat tagcttacag
    19561 cagttgcctg aagatttcac tgtaaatgat aaggaccaac aaagtaatta tttaaaagta
    19621 tttacaataa aggtaaaata atgaaaatag tagtaccagt aatgcctcgc agtcttgaag
    19681 aggctcaaga aatagattta tcaaaatttg atagtgttga tattattgaa tggcgagctg
    19741 atgccttacc aaaggatgac attattaatg tagctccagc tatttttgag aaattcgcag
    19801 gtcatgaaat tatttttact cttcgtacaa cgcgtgaagg tggtaatatt gtcttatctg
    19861 atgctgagta tgttgagtta atccagaaaa ttaattctat ctacaatcca gattatattg
    19921 attttgagta tttttcacat aaagaagttt ttcaagaaat gctagaattt ccaaatttag
    19981 tcctgtctta tcacaatttt caagagacac cggagaatat tatggagata ttttcagaat
    20041 taacagccct agcaccacga gttgtgaaaa tcgcagtaat gccaaagaat gaacaagatg
    20101 tcttagacgt tatgaattac actcgcggtt tcaagactat taatcctgat caagtttatg
    20161 cgacggtatc tatgagtaaa attggacgta tttctcgttt tgctggtgat gtaactggat
    20221 ctagttggac atttgcatat ttagattcat ctatcgcacc cggacaaatt actatttcag
    20281 agatgaagcg tgtcaaagca ttgcttgacg ctgactgata cgagataaca aaatgttaaa
    20341 tatactgatg agatccctag caattgctta gggtttttgt cttattaaaa caaacatgag
    20401 aggacattag aatgcaggtt gaagttgact taccgaatca cccttatcat attaaaattg
    20461 aagaaggatg tttttctgag gctggtgact gggtatcaca tttatggcag aaacaaatga
    20521 taactattat tacagatagt aatgtagaaa tattatacgg ggagtcgctt gtcaaccagt
    20581 taaaaaaaca aggttttaca gtacatgttt ttagttttgc tgcgggtgaa gctagtaaaa
    20641 ctttggaagt tgctaatcgt atttatggtt ttttagcaaa gcatcatatg accagaagtg
    20701 atgggatcat tgcacttggt ggaggcgtcg tcggagattt agcagcgttt gttgcttcta
    20761 cttatatgcg gggaatccat tttcttcaaa ttccaactag tttaacagca caggttgatt
    20821 cttccattgg cggaaaaaca ggagtcaaca cttcattcgc taaaaatatg gtaggtactt
    20881 ttgcacaacc tgatggtgta ctgatagatc ctgttactct aaaaacattg ggcaatcgtg
    20941 aattagttga aggtatggga gaagtcatca aatatggctt gattgatgat ataaaactct
    21001 ggcatatctt agaagagatg gatgggtcta ttgatagtat ccttgataat gcattagcta
    21061 ttatttatca ctcttgtcaa gttaaacgaa agcatgtttt agctgatcaa tatgataaag
    21121 ggttaaggat gcatttaaat tttggccaca ctatcggtca tgctatcgaa gtacatgctg
    21181 gctatggaga aataatgcat ggagaagctg ttgcaattgg aatgatccag ctttctcgtg
    21241 tggctgagcg taaaaatctt atgccaagag gtatcagtca agatatttat aatatgtgtt
    21301 taaaatttgg ccttccggtc cactatgcag aatgggataa ggacgtctta tttaacatat
    21361 tgagtcatga taaaaaagca agtggacaat ttattaaaat tgttatactg cctcaattag
    21421 gtagtgcaac agtccaccaa atacctttag aagaaatgag agattatttg gagaaataga
    21481 tgagatattt aactgctggt gagtcacatg gacctagttt aacagctatt atcgaaggga
    21541 ttcctgcggg actgaaatta tctgctaaag atattaatga agatttaaaa cgccgccaag
    21601 gtggttatgg tcgtgggaat cgaatgaaaa ttgaaactga tcaagtgatc attagttctg
    21661 gtgtgcgcca tggaaaaacc ttgggttcac ctattacact gacagtaact aataaggatc
    21721 atagcaaatg gctggacatc atgtcagtcg aagacattga agagcgtcta aagcaaaaac
    21781 gtcgtattaa gcatcctaga cctgggcacg cagatttagt aggtggaatc aaataccgat
    21841 ttgatgattt gagaaatgct ttagagcgtt cttcggctcg tgagaccaca atgcgagtag
    21901 ctattggtgc gattgctaaa cgtatcttga aagaaattgg tatcgaaatt gctaaccata
    21961 ttgtagtttt cggtggcaag gagattactg tccctgataa gctaacagtt caacaaatca
    22021 aagtgttatc tagtcaatcg caagtagcta tcgtcaatcc atcatttgaa caagaaatta
    22081 aggattatat tgacagtgtg aaaaaggctg gagatactat cggaggagtg attgaaacta
    22141 tcgttggagg agttcccgtt ggtttaggct cttatgtaca ctgggataga aagttagatg
    22201 ctaagatagc gcaagcagtt gtatctatca atgcttttaa aggtgttgaa tttggtctag
    22261 gttttaagtc agggttttta aaaggaagtc aagtaatgga ctccatatca tggacaaagg
    22321 accaaggtta catacgtcag tcaaataatt taggcggttt tgaaggcggt atgactaatg
    22381 gagagcctat tgtcgttagg ggagtaatga agccaattcc tacgctttac aagccactaa
    22441 tgtcagttga tattgatact cacgagcctt atagagctac agtggaacgt tctgatccca
    22501 cggcacttcc ggctgcaggt gtcgttatgg aagctgtagt agccaccgtc ctggtgacag
    22561 aagtgctaga aaaattttct tcagacaaca tgtatgaact aaaagaagct gtaaaactat
    22621 atcgcaatta tgttaatcat ttttaagtga aaactagtag cattcaattg caaaaaaagg
    22681 tataaaagtc tacaatagtg gtaagttcaa gtagaaaaag gagtaactta tggctaatgt
    22741 atatgattta gcaaatgaat tagaacgtgc tgttcgtgct ttaccagaat accaagcagt
    22801 tttaactgca aaagcagcta ttgaaaatga tgcggatgca caagtgcttt ggcaagactt
    22861 tttggctacc caatcaaaag ttcaagaaat gatgcaatct ggccaaatgc caagtcaaga
    22921 agaacaagat gaaatgtcta aacttgggga aaaaattgaa tccaatgacc ttttaaaagt
    22981 ttattttgac caacaacaac ggttgtctgt ctatatgtct gatatcgaaa aaattgtctt
    23041 tgcacccatg caggacttga tgtaatagta taaaagagag agctccttga gttttctctt
    23101 ttatgctatt tacattcaga gcatcctaat tgctgcgctt ttacattatt gcattatcct
    23161 ataaaaatgt atttttatac tttttttgag agcggctatt tatttttaga cttagtcatg
    23221 tctctaaata cccctagtta gtgataaaaa caaggttttt atagg
//
LOCUS       DATRME010000027        22369 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRME010000027 DATRME010000000
VERSION     DATRME010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22369
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..372
                     /locus_tag="VBC23_001033"
     CDS             <1..372
                     /locus_tag="VBC23_001033"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6842990.1"
                     /translation="QIKEFIPNIVSLTYLRNYGAAFSILQNQQWLFTLITIFVVCVAI
                     IYLMKHINGSYWLLISLTLIISGGLGNFIDRLRLGYVVDMVHLDFINFAIFNVADSYL
                     TIGIICLMIALWKEESNGNHN"
     gene            356..1246
                     /locus_tag="VBC23_001034"
     CDS             356..1246
                     /locus_tag="VBC23_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6842991.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            1422..1943
                     /gene="pyrR"
                     /locus_tag="VBC23_001035"
     CDS             1422..1943
                     /gene="pyrR"
                     /locus_tag="VBC23_001035"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6842992.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            1992..3062
                     /locus_tag="VBC23_001036"
     CDS             1992..3062
                     /locus_tag="VBC23_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6842993.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            3118..4716
                     /locus_tag="VBC23_001037"
     CDS             3118..4716
                     /locus_tag="VBC23_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6842994.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            4918..6162
                     /locus_tag="VBC23_001038"
     CDS             4918..6162
                     /locus_tag="VBC23_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6842995.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            6165..6875
                     /locus_tag="VBC23_001039"
     CDS             6165..6875
                     /locus_tag="VBC23_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6842996.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            6890..8137
                     /locus_tag="VBC23_001040"
     CDS             6890..8137
                     /locus_tag="VBC23_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6842997.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            8266..8538
                     /gene="rpsP"
                     /locus_tag="VBC23_001041"
     CDS             8266..8538
                     /gene="rpsP"
                     /locus_tag="VBC23_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6842998.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            8548..8790
                     /locus_tag="VBC23_001042"
     CDS             8548..8790
                     /locus_tag="VBC23_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6842999.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            8922..10433
                     /locus_tag="VBC23_001043"
     CDS             8922..10433
                     /locus_tag="VBC23_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6843000.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSLKNDSGVAIQDILEIGMNNKQYHELLLQAIIEKRDQKVFEYISKMKHKIL"
     gene            10541..10612
                     /locus_tag="VBC23_001044"
     tRNA            10541..10612
                     /locus_tag="VBC23_001044"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10573..10575,aa:Gln,seq:ttg)
     gene            10621..10692
                     /locus_tag="VBC23_001045"
     tRNA            10621..10692
                     /locus_tag="VBC23_001045"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10654..10656,aa:Glu,seq:ttc)
     gene            10755..11273
                     /gene="rimM"
                     /locus_tag="VBC23_001046"
     CDS             10755..11273
                     /gene="rimM"
                     /locus_tag="VBC23_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6843001.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVNLSNQRV
                     QVELMEGLDDED"
     gene            11260..12015
                     /gene="trmD"
                     /locus_tag="VBC23_001047"
     CDS             11260..12015
                     /gene="trmD"
                     /locus_tag="VBC23_001047"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6843002.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            11996..12988
                     /locus_tag="VBC23_001048"
     CDS             11996..12988
                     /locus_tag="VBC23_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6843003.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            13125..14195
                     /locus_tag="VBC23_001049"
     CDS             13125..14195
                     /locus_tag="VBC23_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6843004.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            14208..15131
                     /locus_tag="VBC23_001050"
     CDS             14208..15131
                     /locus_tag="VBC23_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6843005.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            15343..16977
                     /locus_tag="VBC23_001051"
     CDS             15343..16977
                     /locus_tag="VBC23_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6843006.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            17121..18206
                     /locus_tag="VBC23_001052"
     CDS             17121..18206
                     /locus_tag="VBC23_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6843007.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            18231..18356
                     /locus_tag="VBC23_001053"
     CDS             18231..18356
                     /locus_tag="VBC23_001053"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO6843008.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            18421..19230
                     /locus_tag="VBC23_001054"
     CDS             18421..19230
                     /locus_tag="VBC23_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6843009.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            19227..20138
                     /gene="pfkB"
                     /locus_tag="VBC23_001055"
     CDS             19227..20138
                     /gene="pfkB"
                     /locus_tag="VBC23_001055"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6843010.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            20135..22099
                     /locus_tag="VBC23_001056"
     CDS             20135..22099
                     /locus_tag="VBC23_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6843011.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
     gene            22288..>22369
                     /locus_tag="VBC23_001057"
     CDS             22288..>22369
                     /locus_tag="VBC23_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO6843012.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFA"
BASE COUNT         7531 a         3318 c         4439 g         7081 t
ORIGIN      
        1 caaattaagg aattcattcc aaatatcgtt agtctaactt atctgagaaa ttatggtgct
       61 gcattttcta ttttgcaaaa tcaacaatgg ctttttacct taattacaat ctttgttgtt
      121 tgtgttgcta ttatttattt gatgaaacat attaatggaa gttattggtt attaatttct
      181 ctaaccttaa tcatctctgg tggattagga aactttatag atcgcttaag attaggttat
      241 gtcgttgata tggtgcattt agattttatt aattttgcta tctttaatgt agcagactca
      301 tatctgacaa tcggtattat ttgcttaatg attgcacttt ggaaagagga aagtaatgga
      361 aatcacaatt aaaatagcag gagttaggct agataaagct ttagctgata attctgaact
      421 gtcgcgtagt caagctaatg aagaaattaa aaaagggatt gtgttagtaa atggacaagt
      481 aaaaaaagcg aaatatactg ttcaagaagg agatcggatt acttttgata ttcctaaaga
      541 ggaagtacta gattatcaag ctgaaaatat ccctttagat attatctatc aagatgatga
      601 cgttgctgtg gttaataagc cgcaagggat ggttgttcac ccttctgcag gccacagttc
      661 gggcacctta gttaacgcct tgatgtatca tattaaggat ttatcatcaa ttaatggcgt
      721 tgttcgccca gggatcgttc atcgtattga taaagatact tctggtttat tgatggttgc
      781 aaaaaatgat agagctcacc aagtgttggc tgaagagttg aaagataaaa aatcgcttcg
      841 taaatattta gcaattgtgc atggtaattt accaaatgat cgaggcgtga ttgaggctcc
      901 gattggtaga agtgataaag atcgtaagaa gcaagccgtg actgctaaag gtaagccagc
      961 aataactcgt tttcatgttt tagaacgttt tggtgattat accttagttg aattgtcatt
     1021 ggagacaggt cgaacgcacc aaattcgggt tcatatggct tatattggcc atcctctagc
     1081 cggtgatcct gtctacggtc cccgcaagac tttagggggt aaaggacagt ttttgcatgc
     1141 acaaacacta ggttttacgc accctagtaa cggagagaat ttgatttttt cagtagaggt
     1201 tccggaaatt tttcagacaa ctttagacaa gttgagaaaa aattaagttt ttcttgaaaa
     1261 atacttttac catttgctat aatataagca aatcattcct ttaaccttat ccagtgagat
     1321 agttaaggag aaacttcaga ctaaatcaat gcctgagcgg ctttgtcatt ctagagagtc
     1381 tccttttgaa ggggctcttt tatcttagag aggatactgc aatgaaaaga aaagaaatta
     1441 ttgatgatgt gaccatgaaa cgtgctatta cacgtattac ctacgaaatt atagaacgta
     1501 acaaaaattt agataatatt gttttagcag gcattaaaac aagaggtgtt tttttagcca
     1561 aacgcattca agaacgtttg aagcaattag aaaatttaga tattcccgtt ggagaattgg
     1621 atactaaacc tttccgtgat gatatgaaag ttgaagttga caccacaact atgccagttg
     1681 acatcactga taaagatatt atattaattg atgatgttct ctatacaggt cgcactattc
     1741 gagctgctat tgataatctt gttagtttgg ggcgtccgag tcgtgtcagt ttagctgtat
     1801 taattgaccg tgggcatcgt gaactaccca ttcgtgcaga ttatgtgggg aaaaatattc
     1861 caacaagtca atttgaagaa attctagttg aagtgatgga acatgatggt tacgatcgtg
     1921 tgagtattat tgatccgagt taagtagacg actattattt acgatgattt tatagaaggg
     1981 gaagaaccac aatggcaaaa aaattgttaa ttctagagga tggtacggtt tttgaaggac
     2041 tatcctttgg ttcatctctt gatgtaactg gcgaattagt tttttgtacg gggaataccg
     2101 gctatcagga aataataacg aatccttcac ataatggaaa aattttagtt tttacctcac
     2161 cgctaatagg aaattatggt attcatcgta gttactctga agccattatt cccacttgtt
     2221 taggagttgt tgttgcagag tattccagat gtgtaagtag cgatactagc aagatgaatc
     2281 tagatgaatt tttgaaaatg aaaaaggttc ctgcaatgtc gggggtcgat acaaggtatt
     2341 tgatgcaagt tattaaagaa aaaggatttg taaaagctac tttagcagaa gcaggtgatg
     2401 ttttatcaca tttacaagat caattaatag cgacagtatt accgacaaat aatgtggaac
     2461 aagtttcgac aaaaacagct tatccgtcgc ctgctagcgg tcgaaatatt gttgtacttg
     2521 attttggcct taaacattct gttttgagag aattgtctaa acgtcagtgt gacgtgactg
     2581 tcattcccta caatacgtct ttagaaggta tcaaaaatct atacccagaa ggtatcatat
     2641 tatctaatgg tccgggaaat ccagaaaagc ttcaggaagt cttaaacact attaaagaat
     2701 tacaaaaatc tgttcctatg ttaggtattg gtttggggca tcaattaatt gccatggcaa
     2761 atggtgcaga aattatgaga ctcccagttg ctaagaaggg gccaaattat cccatgagag
     2821 atattgctac ggggcgttta gaaacagtta ttcagtttaa tcattttacc gttaaccggc
     2881 taaatcttcc gcatgattta ctggtaacac acgaaggttt gaatgatcaa gagattgttg
     2941 ctctaaggca ccgctcattt ccagtgatgt ccgtgcaatt ttacccagag gctgctccag
     3001 gccctcatga tgtgacctat ttttttgatg aatttttaga aatgattgga tcacgcgctt
     3061 aaacataagg tattatttaa agtcagtcgg tgagctgatt aataaggagg tgcgtatatg
     3121 ctaaatacaa aggataaaca gcctattttg cttattattt tcggacagaa aatgaaagaa
     3181 tatgacgatt taattagatc aactgttgag aggttatcta aaaggaatag acctgttagt
     3241 attcttatct cgaaggagct aaaccatgtg attcaccatt atgatgcaca atttgaaaat
     3301 cttgagacgc ttgtggggta tataaataca catgaagata gtttgattta tcccctttgc
     3361 ttagataaaa atcatgagct atttcaagag tttttagcgt ggcagagtca ttattcaagg
     3421 cagtatatca gtgataattt atcagaaaaa agtgttcagg caatagacaa taatcaagac
     3481 tttgtgcaag tagattgttt agtaatgaga gactctttga ataattgttt atatgtctct
     3541 gatttagaat atattgaatc taataaaaca actggtaagt ctctagcgat tgtaccaagt
     3601 caaactttat ccgatgctgc taggcaaaca attcgagatg ttgcatttga tgtgtgtcga
     3661 aaggcaaaca ttattggagt atgttacttt agtttcttaa tagatttgaa cagtttggat
     3721 tatcatataa ttagcttatc atctggtttg tcttatcaaa gcattttatt tgagactatt
     3781 acgacctatc ctgttttaga gatagcaact aaattgacag tgggatatac ttttagtcaa
     3841 ctgaagcatt cttattaccc aaatacctct gcctttttag agccgcaatt agactatgta
     3901 gctaccgtat cttttagttt tgaaaaagtt gactatatct tttttgctcg taatatagag
     3961 caattatttt taaatttact cgaggctagc agtcatgatc attttccatt tctgagtgat
     4021 attagcgagg aagatttgat gtttgctttg attcagaaaa aagaaaatcg tctagcttat
     4081 cttttagagg cttttagacg tggctttgat ttgtatgatt tatcctcagt tactaaaatc
     4141 aacccttttt acttagataa atgtttacat attgtagagt tgtatgaaaa cctgaataaa
     4201 agccaatata atgtagatat ttacaaggat gctaaaaggt atggtttttc ggatgactat
     4261 attgctagta gttggcaaat atctttgata gacatgttag aatatcgaaa aaaacatagt
     4321 gtcgcacctg ttctgaagca agtcgaacaa agttctggtg ttctaactgg ccatcaaatt
     4381 cagtattttc gaagctatga ttggcattca gactacagtt ctagcggttg tcaaaaggct
     4441 ttgataatgg tagacaaggg ttattcccta gtaaaattaa atgagttaat taagcaaatc
     4501 aaacaaactc atttagaatt attaattgtt accaatcaac ctctcctcat agaacaatta
     4561 aatgatacat ctataatttt tgacactata ggtattgaga ccatattaac aattatgggt
     4621 atagaagagg tggaagaagt gtatttattg ggagaaagtc aagcagacat gttttcaaaa
     4681 atgaaaaatt tttctatctc ttgtcagtct atttagcaat tcttaataaa agctgagata
     4741 tagatctcag cttttatttg tggtaaaaag taaagtactt tcaattttgt aagttctttc
     4801 tatgagcaaa atagtatact taatgagtaa aaatattatt tttaaagaat tttcttgaca
     4861 atattattta aaaataatac acttgttcta tattaataat acaaacagga gatggacatg
     4921 agtaaacgac aaaatttagg gattagtaaa aaaggagcaa ttatatcagg gctctcagtg
     4981 gcactaattg tagcagtagg tggcttttta tgggtacaat ctcaacctaa taagagtgca
     5041 gtaaaaacta attacaaagt ttttaatgtt agagaaggaa gtgtttcgtc ctcaactctt
     5101 ttgacaggaa aagctaaggc taatcaagaa cagtatgtgt attttgatgc taataaaggt
     5161 aatcgagcaa ctgttacagt taaagtgggt gataaaatca cagctggtca gcagttagtt
     5221 caatatgata caacaactgc acaagcagcc tacgacactg ctaatcgtca attaaataaa
     5281 gtagcgcgtc agattaataa tctaaagaca acagggagtt ttccagctat ggaatcaagt
     5341 gatcaatctt catcatcatc acaaggacaa gggactcaat cgactagtgg tgcgacgaat
     5401 cgtctacagc aaaattatca aagtcaagct aatgcttcat acaaccaaca acttcaagat
     5461 ttgaatgatg cttatgcaga tgcacaggca gaagtaaata aagcacaaaa agcattgaat
     5521 gatactgtta ttacaagtga cgtatcaggg acagttgttg aagttaatag tgatattgat
     5581 ccagcttcaa aaactagtca agtacttgtc catgtagcaa ctgaaggtaa actccaagta
     5641 caaggaacga tgagtgagta tgatttggct aatgttaaaa aagatcaggc tgttaaaata
     5701 aaatctaagg tctatcctga caaggaatgg gaaggtaaaa tttcatatat ctcaaattat
     5761 ccagaagcag aagcaaacaa caatgactct aataacggct ctagtgctgt aaattataaa
     5821 tataaagtag atattactag ccctctcgat gcattaaaac aaggttttac cgtatcagtt
     5881 gaagtagtta atggagataa gcaccttatt gtccctacaa gttctgtgat aaacaaagat
     5941 aataaacact ttgtttgggt atacaatgat tctaatcgta aaatttccaa agttgaagtc
     6001 aaaattggta aagctgatgc taagacacaa gaaattttat caggtttgaa agcaggacaa
     6061 atcgtggtta ctaatccaag caaaactttc aaggatgggc aaaaaattga taatattgaa
     6121 tcaatcgatc ttaagtctaa taagaaatca gaggtgaaat aatcgtgaca actaatcgaa
     6181 aagaactgat tagattgcat cagattgtta aaagttatca aaatggggat caaaaacttc
     6241 aagtcttaaa gaacattgat ttgacggttt atgaaggtga atttcttgca atcatggggc
     6301 catctggctc agggaaatcc acactaatga atattattgg cttgttggat tccccaactt
     6361 caggagatta tagtttaaat ggtaaacgtg tcgaagaact aagtcaaaca aaattagctc
     6421 aagttcgaaa taaggaaatc ggttttgtct ttcaacaatt tttcttgtta tctaaactaa
     6481 cggctttgca aaatgttgaa ttacccttaa tttatgcggg tgtagctcct aaaaagcgta
     6541 aaaatttagc aaaacaattt ttagataaag ttgaattgag agagcgtatg aaccatctgc
     6601 caacagaatt atctggggga cagaagcaac gtgtagcaat cgcgcgtgct ttggtgaata
     6661 gcccatctat tattctagca gatgagccga ctggtgcctt ggatacaaag acgggtgagc
     6721 aaattatgca gttcctaacg gaattaaatc aagaaggtaa aaccattatc atggtaactc
     6781 atgaaccaga aatcgccgat tatgcgacac gtaaaattgt tattcgtgat ggcgaaatta
     6841 cagcagatac tactgatagt attagaattg attaagggga gattcgatta tggaaaattg
     6901 gaaatttgcc ctatcatcga ttctagggca caagatgcgt gcttttctga ccatgttagg
     6961 gattatcatt ggggttgcat cagttgtgtt aattatggcc ttaggtaagg gcatgaaaga
     7021 ttcggttaca aatgagatta caaagtctca gaagaatctt caaatttatt ataaaacaaa
     7081 agaagaccaa aaaaatgagg ataactttgg agctcaaggg gctttcatgc aaggtagtga
     7141 taccaaccgt aaggaaccaa ttattcaaga atcatggtta aaaaagattg ctaaggaagt
     7201 agatggtgtt agtggctact atgtaaccaa tcaaacaaat gctccagtag cttaccttga
     7261 aaaaaaggca aagacggtta atataacagg aattaaccgc acttaccttg gtatcaaaaa
     7321 gttcaaaatt aaaagtggtc gtcaattcca agaggaggat tataaccaat tctcgagagt
     7381 tattttacta gaagaaaaat tagctcagag attatttcag acaaatgaag cagcgttaaa
     7441 taaagtggtg actgtaaaaa ataaatctta cttagtagtg ggggtttatt cggatccaga
     7501 agctggctca ggtttatatg gaagtaattc tgatgggaat gctattttaa ccaatacaca
     7561 gttagcttcg gagtttggtg ctaaagaagc agaaaacata tattttcatc ttaatgatgt
     7621 gtcgcaatcc aatcgcattg gtaaagaaat aggcaaacgt ttaacagaca ttagccatgc
     7681 taaggatggg tactatgata attttgatat gacatctata gttaaatcta tcaataccca
     7741 agttggtatt atgacaggtg ttattggagc gattgcagca atttctctcc tagttggtgg
     7801 tatcggagtg atgaatatta tgttagtctc tgtaactgag cgcacgagag aaattggcct
     7861 tcgtaaagcc cttggggcaa ctcgacgtaa aattttagct caatttttaa ttgaatcaat
     7921 ggtcttaacg attttgggtg ggttaattgg gttattacta gcctatggag gtacgatgtt
     7981 aattgctaat gcgcaagata agattactcc ttctgtgtca cttaacgtgg ctataggcag
     8041 tttaattttc tcagcattta ttggaattat atttggttta ttacctgcta ataaagctag
     8101 caaattaaac ccaattgatg ctttacgata tgaataaaaa aacatgtact ctgtcaagat
     8161 tttttcttga cagagtttgt atttatgtta tacttagtaa ggtaaagttg ttagttaagc
     8221 tatcaacgac caaatataaa gaaaaagaaa agagactatt taaaaatggc agtaaaaatc
     8281 cgtttaactc gtatgggttc taagaagaaa cctttctacc gtatcaatgt tgcagattca
     8341 cgtgcaccac gtgacggacg ttttattgaa actgttggaa catacaaccc attagttgca
     8401 gaaaaccaag ttacaatcaa agaagaacgt gttcttgaat ggttatcaaa aggtgcacaa
     8461 ccttcagata cagttcgtaa ccttctttca aaagctggag ttatgactaa attccacgat
     8521 caaaaattct caaaataata aaagcctatg gataccattg aaaatctcat tattgctatt
     8581 gtgaaacctt tgatttcaca accagatcag ttaaccataa aaattcagga tggcccagaa
     8641 tttttagagt accatcttga tttagatacc caagacatcg gtcgtgttat cggcaaaaag
     8701 ggtcgtacaa ttacagcgat aaggtcgatt gtctattcgg taccaacaca agggaaaaaa
     8761 gtaagactca tcattgatga gaaagaataa tttattctaa aagataggaa cttcctgtct
     8821 tttttgttat atctacacaa aaattgtgag ttttttagtc ttttagaata taatggatgt
     8881 gaaagggaat tttctgattt tttattaagg aggataagat attgctattt gattttctcg
     8941 aagaaaaaat aaaactgaaa gcatacctga tgaccgcctt acttgatgat ggagaaatta
     9001 accttgatca ctttaaaaca gaatatgctt tgagtgaaaa gattttacaa ggtcttattt
     9061 cggaactaca aatactttat tctgatttct accttgttgt caaaagtaga acaatggtat
     9121 tgttccatta cgaaacactt tctaagctag agactttgca taccatttat cgagagtcta
     9181 atacgttaca gtttttggca tattttattg ctcctcaaaa tgtcagtttt tctgaattca
     9241 cggctaagaa attcatatcc gtcgcaagtg cctaccgaat aaagaaaaac tgtaaacttt
     9301 atttggagag tgttaagtta tcaatacaaa aaaataaagt gacaggtcca gaatatcaga
     9361 ttaggctgct gattgctttg ttacagtatc attttggttt tattatttat ccttttgatg
     9421 ctacaagtga ggtaatgata agggatttta ttgaatactc gaattttaaa gggtgtgact
     9481 acgaccttaa agacttatct gaagaatatt actattttta tattttggtg aatttagttt
     9541 ggaaaagaaa agaatataaa gtagaaattc ctcaaacaga atcctttaat caactaaaaa
     9601 aatctattct ctacatggag ttatccaaag cagctcaaaa gagtattgct atacatcttg
     9661 gtatagagtt gaatgaaggt ttacttgatt atctatattt agtgttttgt attagtgatg
     9721 gtcctctatt tagcaatcga gatgatatca atatcaacga agaatttatt tttgcttctt
     9781 ataagatatc aaagtctaaa gcctttaaga atttttgtgg tcgattattg ggtagagaat
     9841 ttattgactc gcagcatttt aaagatatta atgcctattt tttgagacat tttatttgtt
     9901 attgttatta ttttattcct gatttttatt ttttaaatac atcaaggctg agctattcta
     9961 aggatttata tcacctatta gataaaggtc tggctgatat ttttaacctt aaaggaggga
    10021 atttaacttt ttcaaaacat gagacagtgc tactaacaat gcaattatct aacttaatag
    10081 agactttctt agctcctctt tctgtatatg taatttcatc tagtaatatt agattacaaa
    10141 cctatcaagt aatgttaaac caatatttta catctaagat agcggaattc ttttttgtaa
    10201 actatcaaac gactcagata gatgaaaaat tactaaaaaa agcagatatc attattgctg
    10261 agagacgcta tatatcttct ttaaaaaatg actcaggtgt ggctatacaa gatatattag
    10321 aaattgggat gaataataag cagtaccatg aactactttt acaagctatt atcgaaaaaa
    10381 gggaccaaaa agtttttgaa tatatttcaa aaatgaaaca taaaatactt tgatagactc
    10441 tatatgagct ttattaaaag ggataaaatc gaaatttaaa atttaattcg aaaagatatt
    10501 gacagtaatc tagaatttcg atatactaga ttttgtcttt tggggtatag ccaagcggta
    10561 aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc agtttcacat
    10621 ggtccgttgg tcaaggggtt aagacaccgc cttttcacgg cggtaacacg ggttcgaatc
    10681 ccgtacggac tatcgtggcc agttgcattt tagcggctgg tttttctttt attttcaaaa
    10741 atatcggagt tactatggaa tactttaacg ttggaaaaat tgtcaataca caaggattac
    10801 aaggcgaaat gcgtgtatta agcgtgactg actttgtaga agaacgcttt aaaaaaggtc
    10861 aagtgttagc tttattcgat gaaaagaatc agtttgttat ggatattgaa attgctagtc
    10921 atcgtaagca gaagaatttt gacattataa aattcaaagg aatgtatcat attaatgata
    10981 ttgaaaaata taaaggtttc acactgaagg tggcagagga tcaactgagc gaccttaaag
    11041 atggtgaatt ttattatcat gaaattattg gcttagatgt ctatgaaggg gaagagctga
    11101 ttggaaaaat caaagaaatt ttacaaccag gagcaaatga cgtttgggtt gtggaacgtc
    11161 atggaaaacg tgatctttta ttaccctata tcccgccagt agtattagaa gtgaaccttt
    11221 ctaatcagcg tgttcaagtt gaattgatgg aaggtttaga tgatgaagat tgatatttta
    11281 actttatttc ctgaaatgtt tgcccctctg gaacattcta tagttggtaa ggcaaaagaa
    11341 agaggacttc ttgaaattaa ttatcacaat ttcagagaaa atgctgagaa gtctcgacat
    11401 gttgatgatg agccttacgg tggggggcaa gggatgcttt tacgtgctca acctattttt
    11461 gatacaattg ataaaataga tgctcaaaaa gcacgtgtta ttttgctaga cccagcgggc
    11521 agaacatttg atcaggattt tgcagaagaa ctatccaaag aagatgaatt aatttttatt
    11581 tgtggtcact atgaaggtta tgatgaacgt ataaaatcat tagtaacaga cgaagtatct
    11641 ttgggagatt ttgttcttac gggaggagaa ttggcggcta tgacaatggt cgatgctacc
    11701 gttcgtctga ttccagaagt tataggtaaa gaaacaagtc atcaggatga ttcattttca
    11761 tcgggcctat tagaatatcc tcagtatacg cgcccatatg attatttagg catgacagtt
    11821 cctgatgttc tcatgagtgg tcatcatgaa aatatccgaa aatggcgttt agaacaaagt
    11881 ttgcgaaaaa cactagaacg tcgaccagac ttgttagaaa attatgcaat gacggatgaa
    11941 gagagactta tcttagagaa aattaaaaca gaaatagaaa gaacagatac agtaaatgaa
    12001 caaaacaatt tatgatatca ctattgtagg aggcggacca gtaggtctct ttgctgcttt
    12061 ttatgctgga ctacgcggag tatccgttaa aattattgaa agcctttcgg aattaggagg
    12121 ccagccagct atcctttatc ctgagaaaaa aatctatgat attcctggtt atccggtgat
    12181 tactggacgt gaattaattg ataaacatat tgaacaatta gagagattta aagactctat
    12241 tgaaatctgt ttaaaagaag aagttttatc atttgaaaaa gtagatgatg ttttcacaat
    12301 tcaaacagat aaagatcaac atttgtcacg tgctattgtt tttgcttgtg gcaacggtgc
    12361 ttttgctccg cgtttactag gtttggaaaa tgaagaaaat tatgcagata ataatttatt
    12421 ctataacgtt acaaagttag aacagtttgc aggtaaacat gttgttattt gtggtggagg
    12481 cgactcagct gttgattggg ccaatgaatt agataagatt gcagctagtg tagcgattgt
    12541 tcatcgtaga gatgctttta gagcgcatga gcatagtgtg gatatactaa aggcatcagg
    12601 tgttagaatt ttaacccctt atgtcccaat tggtttaaat ggagatagtc agagagttag
    12661 cagcttggtc gttcaaaaag ttaaaggtga tgaagtaatt gaattacctt tagacaactt
    12721 gattgttagt tttggctttt ctacttctaa taaaaattta cgttattgga atttggatta
    12781 taaacggtct agtatcaatg tttcttcact ttttgagacg actcaagaag gcgtgtatgc
    12841 tattggagat gctgctaatt atccaggaaa ggtagaattg attgcaacag gatatgggga
    12901 agcaccagtc gctataaacc aagctatcaa ttatatttat cctgatcgag ataaccgtgt
    12961 agtccattca acatcattga taaaataact ataaataaaa ctgataagtt tataaaaaag
    13021 gattatatat attgttaaaa aaataacaaa acttcctatt tattttgtga tataattaac
    13081 tttgtcgtta tgaatgatat taaataaatg aagcgaggta ttttatgaca aatacggtaa
    13141 caccaaaaga gacggctggc tcttttatca ataaagtgtt aggtgggaca gcaactgcaa
    13201 tcgttgtagc actcatccca aatgctatcc tagccacttt tttgaaacca tttttatctt
    13261 acggacttgc tgcagagttc ttacacatcg tgcaggtatt tcaatttttc acaccaatta
    13321 tggcaggatt tttgattggt caacagttta aattcacccc aatgcaacag cttgctgttg
    13381 gaggtgctgc atatattggt tctggtgcgt gggcttacac agaagttatt caaaaaggag
    13441 ttgctactgg tagtttccaa ttgcgtggga ttggtgatct catcaatatg atgctaacag
    13501 cagctttagc agttttagca gttaagtggt ttggtaataa atttggttcc ctaaccatta
    13561 tcttgttacc tattatcata ggtacaggcg taggctattt aggatggaaa ttactccctt
    13621 acgtttcata tgtgacaaca cttattggtc aaggaattaa ttcttttact actttacaac
    13681 caattgcgat gtcaattcta attgcgatgg ctttttcaat gcttattgta agtcctattt
    13741 caacagtagc gattggtcta gcgattggtc ttaatggtat gtcagcaagt gcagcttcaa
    13801 tgggagttgc ttcaacaaca gcagtgctag tttgggcaac gatgaaggct aataagtctg
    13861 gagttccaat tgctattgct ttaggtgcaa tgaagatgat gatgccaaat ttcctaaagc
    13921 atccagtaat ggcgattcca atgcttatga ctgctacagt aagttctcta acagtcccat
    13981 tgtttaaatt ggtggggaca ccagcttcgt ctggttttgg gcttgttggt gcagtaggtc
    14041 cgattgcttc atttgaagca ggagcaagta tgttgattgt tatcttatct tggctcgtta
    14101 ttccttttgc agtaggtttt gtatcacaca aaatttgtaa agacattctg aaactttata
    14161 aagacgatat tttcgttttt gaaggtcaaa attaatttgg aggttgtatg ttagtttata
    14221 ttgctggttc aggcgctatg ggatgtcgtt ttggatatca aatttctaaa acaaatcatg
    14281 atgttatttt acttgataat tgggcagacc atattatggc tattaaagaa aatggcttga
    14341 aggtaacagg tgatacagag gaccttgtta aacttcctat catgaagcca actgatgcga
    14401 cagaagaagc agatttaatt attttgttta ctaaagcaat gcagttgcca aatatgcttc
    14461 aagatattaa aaaaattatt ggtaagaaaa caaaggtctt atgtctttta aatggattag
    14521 gacatgaaga tgtcattcgt caatatatcc ctgaacacaa tattttgatg ggagtaacag
    14581 tttggactgc agggttgaaa ggaccagggc atgctcatct ggaaggagtt ggttctgtaa
    14641 atcttcaaag tatcgatcct aacaatcaag aagcaggtca tcgcgttact gaattgctta
    14701 atgaagcaaa attacaggct acttatgacg aaaatgtttt gccaaatatt tggagaaaag
    14761 cttgtgttaa cggaacgatg aattcaactt gcgccttgct agattgtaca attggtcaac
    14821 tctttgctag tgaagacggc gtaaatatgg tacatgaaat tatccatgaa tttgttactg
    14881 ttggaaaagc agaaggtgta gagcttgatg aagaagaaat aacaaagtac gttatggata
    14941 catctgttaa agcagcccat cattaccctt caatgcatca agacttagtt caaaatcagc
    15001 gcttaactga aattgatttc ttaaatggcg cagtgaataa aaaaggtgaa aacctaggca
    15061 ttgatacacc ttactgtcga ttaatcacac aattaattca cactaaagaa aatgtactta
    15121 gcataaaata ataatcaacc gggagaatag tgcccggttt tttgatatgc ttaatgataa
    15181 tgattttgtt atactaagaa taataagagt tgggtatttt tttcgagaag tatttacagt
    15241 ataaaatatt acatttgtat tacaaatatt tctaaaatat gttgacatgt tacaaaaagg
    15301 atattataat agtagtgtaa ataaataagg aagtaggtgt ttatgatgaa aaaaggacaa
    15361 gtaaatgata ctaagcaatc ttactctcta cgtaaatata aatttggttt agcatcagta
    15421 attttagggt cattcataat ggtcacaagt cctgtttttg cggatcaaac tacatcggtt
    15481 caagttaata atcagacagg cactagtgtg gatgctaata attcttccaa tgagacaagt
    15541 gcgtcaagtg tgattacttc caataatgat agtgttcaag cgtctgataa agttgtaaat
    15601 agtcaaaata cggcaacaaa ggacattact actcctttag tagagacaaa gccaatggtg
    15661 gaaaaaacat tacctgaaca agggaattat gtttatagca aagaaaccga ggtgaaaaat
    15721 acaccttcaa aatcagcccc agtagctttc tatgcaaaga aaggtgataa agttttctat
    15781 gaccaagtat ttaataaaga taatgtgaaa tggatttcat ataagtcttt tggtggcgta
    15841 cgtcgatacg cagctattga gtcactagat ccatcaggag gttcagagac taaagcacct
    15901 actcctgtaa caaattcagg aagcaataat caagagaaaa tagcaacgca aggaaattat
    15961 acattttcac ataaagtaga agtaaaaaat gaagctaagg tagcgagtcc aactcaattt
    16021 acattggaca aaggagacag aattttttac gaccaaatac taactattga aggaaatcag
    16081 tggttatctt ataaatcatt caatggtgtt cgtcgttttg ttttgctagg taaagcatct
    16141 tcagtagaaa aaactgaaga taaagaaaaa gtgtctcctc aaccacaagc ccgtattact
    16201 aaaactggta gactgactat ttctaacgaa acaactacag gttttgatat tttaattacg
    16261 aatattaaag atgataacgg tatcgctgct gttaaggtac cggtttggac tgaacaagga
    16321 gggcaagatg atattaaatg gtatacagct gtaactactg gggatggcaa ctacaaagta
    16381 gctgtatcat ttgctgacca taagaatgag aagggtcttt ataatattca tttatactac
    16441 caagaagcta gtgggacact tgtaggtgta acaggaacta aagtgacagt agctggaact
    16501 aattcttctc aagaacctat tgaaaatggt ttaccaaaga ctggtgttta taatattatc
    16561 ggaagtactg aagtaaaaaa tgaagctaaa atatcaagtc agacccaatt tactttagaa
    16621 aaaggtgaca aaataaatta tgatcaagta ttgacagcag atggttacca gtggatttct
    16681 tacaaatctt atagtggtgt tcgtcgctat attcctgtga aaaagctaac tacaagtagt
    16741 gaaaaagcga aagatgaggc gactaaaccg actagttatc ccaacttacc taaaacaggt
    16801 acctatacat ttactaaaac tgtagatgtg aaaagtcaac ctaaagtatc aagtccagtg
    16861 gaatttaatt ttcaaaaggg tgaaaaaata cattatgatc aagtgttagt agtagatggt
    16921 catcagtgga tttcatacaa gagttattcc ggtattcgtc gctatattga aatttaaagc
    16981 taaatctttt tagatttagc tttactaata ttatgtgtgt tggaaaaaac agtggagatt
    17041 tacgtcaaaa ttatattatt tggatagtat cgaatagata gaatgtggag aaaagtagga
    17101 aatagttaag aaaggtattt atgacgcttc gagaattaac aatagaagaa tttaaagaac
    17161 attcaggaaa ttatgattca caatcatttt tacaaacacc tgagatggct aaacttttag
    17221 aaaaacgcgg ctatgatgtt aggtatttgg gatatcaagt agaaaataaa ctagagataa
    17281 tcagtttatc ttatattatg ccagtcactg gtggttttca aatgaaaatt gattcaggac
    17341 cagttcattc aaattctaag tatctaaaac aattttataa agcattgcaa ggctatgcca
    17401 aatccaacgg tgttctagaa ttaatagttg agccttttga tgattaccaa ttattcacta
    17461 gttcgggagt tcctagtaat cagggaaatg ataatctgat tgaagatttt accagttcag
    17521 gttatcacca tgatggttta acaactggtt ttactggtaa atatttatct tggcactatg
    17581 ttaaaaattt agaaggtgtc acttctgaaa cgttactatc ttcattctct aagacaggac
    17641 gagctttggt taagaaagca atgtcttttg gaatcaaggt tcgcgttctt aaacgtgatg
    17701 agctacattt atttaaagag ataacaactt ctacgtcaaa tagacgtgat tatatggata
    17761 agtccttaga ttattatcaa gatttttacg atagctttga aggcaaggct gaatttgtga
    17821 ttgccacttt aaattttaga gaatacgacc ataacttgca aataaaagct gaagcattgg
    17881 aaaataagct taaattgctt gatgaacgtt ttagggaaaa tgcagactct cctaagtatc
    17941 ataggcaacg ctctgaaata attaatcaac tggctagttt tgaaacacga cgacaagaag
    18001 ttcagtcatt tattcaaaag tatgataacc aagacgttgt tttagctggt agtctatttg
    18061 tttatagtct taaggagaca gtttatttct tttcaggatc ctacacagaa tttaataaat
    18121 tttatgcacc agcagttctt caggaatatg ttatgcaaga ggctctcaaa agaggttcta
    18181 ctttctataa cctcctaggt atttagggaa cttttgatgg ttcagatagt attcttcgct
    18241 ttaaacagaa ttttaatggt tatatcattc gtaaaatggg aacttttaac tattatccaa
    18301 gtccttttaa atacaaagga attcagcttc ttaaaaaggt tttgaagaga aattaaagta
    18361 tttgtaaaag ctaatcttta gattaggctt ttttagttta ttgatagttt ttggaagaaa
    18421 atgcttgaca ttgaaagaaa aagagatata attaaattgc aaacgatttc aattcaagga
    18481 gggactatgc taaagtcaaa gagaaaagaa attatcttat cacgacttga gcaaaataag
    18541 agtgttactt tagatgaatt aacgtctatc ttagagactt ctgaatctac agttcgcagg
    18601 gatttagatg aacttgaatc tgaaggtttt ttaaaaaggg tgcatggtgg tgctgaatta
    18661 ccatactcac ttggccagga attaagtaat caagaaaaat ctatcaaaaa cgttcaaaaa
    18721 aaattggata ttgctagaca aactgcaaag ctgatagcaa agcaagatgt tatatttatt
    18781 gatgccggaa ctacgacaga attattaatt gattttctcc ctcacgagca attaacggtg
    18841 gtgacgaatt ctattcatca tgctgcaaag ttagttgata gagggataaa aacaatcatt
    18901 atcggaggag cggttaaaca ttcaacagat gctagtattg gccaggtagc cattaatcag
    18961 attaggcaga taacggttga caaagctttt ctggggatga atggtataga tgaagtttat
    19021 ttaactacac cagatttaga agaagctgct attaaagaag ctattattaa taatagtcaa
    19081 caaaccttta ttctaatgga ttcatctaaa attggacaag ttacatttgc taaagtgaaa
    19141 gagataaatg acattaattt agtaactaat aagactgatt cagaacttat gacaataata
    19201 aaagagaaaa tgaaggtgat tcaagtatga tttatacagt aacattaaat ccatcgattg
    19261 attttattgt tcgtctagat acactattgt tgggtagtgt taaccgtatg actagcgatg
    19321 ataaatatgt aggtggtaaa ggaattaatg ttagtcgcat attaaaacgc ttaaaaattg
    19381 acaatactgc tacaggtttt atcggtggat ttacaggaca ttttgttgaa gatggacttg
    19441 tatcagaagg tatcaaaact gactttgttt ctgttaatga agatacacgt atcaacgtta
    19501 aagtaaaagc aaaaattgaa actgaaatca atggcggggg tcctaggata acaaatgaac
    19561 aacttcatcg tctagagaaa cttctttcga gactaacacc tgaggacaca gtagttttcg
    19621 caggttcagc accagctagc cttggaaata aggtctataa tacattgatt ccaattgcta
    19681 aaaagacagg agcggaagtt gtttgcgatt ttgaaggtca gacactttta gacgctcttg
    19741 cttatcagcc tcttttagta aaaccgaata accatgaatt agcagatatt tttggcgtag
    19801 agctagaagg tctgcctgat atcgaaaaat atgcacataa gattctagat aaaggagcta
    19861 agaatgttat cgtttctatg gcaggggatg gtgcattgtt agtaactcca gaagcttcat
    19921 attttgcaaa accgattaag ggtgaagtga aaaattccgt tggggctggt gattcaatgg
    19981 tagctggctt tacaggagaa tttgttaagt caaaaaatcc agtagaagca ctaaaatggg
    20041 gcgtagcttg tggtacagca accactttct cagatgattt agcaacagct gagtttatcc
    20101 aagatattta taataaagtt gaggtagaaa aattatgaaa atccaagacc tactaaaaaa
    20161 agaagttatg attatggatt taaaagcaac cagtaaagag gctgcaattg atgaaatgat
    20221 tacaaaatta gttgacacag gtgttgtgac aaactttgct atttttaaag atggtatcat
    20281 gaagcgtgag gcacaaacat caactggatt aggtgatggt atcgctatgc cacatagtaa
    20341 aaatgctgcc gttaaagaag ctactgtcct ttttgcaaaa tctgcatcag gtgttgatta
    20401 cgaagcactt gatggacagc caactgattt attctttatg attgctgcgc cagatggtgc
    20461 taatgatacg catttagctg ccttggcaga actatctaaa tatcttttaa aagaagggtt
    20521 cgctgatcaa ttacgtcaag caaaaacgcc agatgacatt attgctactt ttgatagtaa
    20581 tagcatttct caagaaacag tagctccaca aacagtacaa tcaacaagta aaggttcaga
    20641 ttatattgtt gcagtaacag cttgcactac gggaattgcc cacacgtata tggcagaaga
    20701 agctttaaag aaaaaagcag ctgaaatggg tgtaggtatt aaagttgaaa caaatggcgc
    20761 ttctggtgta ggaaataagt tgacttcaag tgatattgca agggctaagg gtgttattat
    20821 tgcagcagat aaggctgtag agatggatcg ttttgatggc aaacctttgg tatcacgtcc
    20881 ggtagctgat ggtattaaaa aaagtgaaga cttaattaac attattctcg ataataaggc
    20941 acaaacctat catgcaaaaa atcaaaatga taaacaatca ggtgaatctg atggaaaatc
    21001 aggcttaggc tctgcttttt ataaacacct tatgggtggt gtatcacaaa tgttaccatt
    21061 cgttattggt ggtggtatta tgattgcgat tgctttcctt tttgataaca tacttggtgt
    21121 accaaaagac caattaagta atttaggttc ataccatgaa attgcagcct tattcaaaaa
    21181 tatcggtggc gcagcatttg catttatgtt accagtattg gcaggttata tcgcttattc
    21241 aatagctgaa aaaccaggac tagtagctgg cttcgtagct ggttcaattg catcaagtgg
    21301 actggcattc ggtaaagtac cttttgcaga aggtgggaaa gcaaccttag cattagcagg
    21361 tgtgccatca ggtttcttag gggctttagt aggtggtttc ttagctggtg gagttatcct
    21421 actacttaga aaattacttt caggtctccc aaaatcgctt gaaggtatta aatcaattct
    21481 tctataccca ttgttaggtg ttttgattac aggtttcttg atgttgctcg taaacattcc
    21541 aatggcagct atcaacacag ctcttaacac tttcttacaa ggtttatctg gtagttcagc
    21601 agtcttgatg ggcttactag ttggtggtat gatggctgta gatatgggtg ggcctgttaa
    21661 taaagcagct tatgtttttg gtacaggtac tcttgcagca actgttgcaa atggcggctc
    21721 tgttgttatg gcagctgtaa tggctggagg aatggttcca ccgttagcag tatttgtggc
    21781 aactttatta tttaaagata aatttaataa tgaagaacgt caatctggtc taacaaatat
    21841 cgttatgggg ttatctttta tcactgaagg tgctattccg tttggtgctg ctgacccagc
    21901 tcgtgcaatc cctagcttta ttgtaggttc agccttaact ggagcacttg ttggtttagc
    21961 tggtatcaag ttgatggctc ctcatggagg gatttttgtc atcgcattaa caagtaatcc
    22021 tttactttat attctattta ttctaattgg agcgatagtc tcaggtgtac tctttggctt
    22081 gttccgtaaa acaaaatagt taatctcaac ttatccaaat ttacattgaa taaaaggtga
    22141 ataaactatc gttattgggt ctattcgcct ttttatgttg taaaagaatg gtagattaat
    22201 cattaataat tgctaatctt ttttcaatag gatatgattt atttaagcaa aaaaatggat
    22261 aatatgattg aggaaaggag gtaagcaatg attaaatgga atgaaaaaga aacaaaacta
    22321 gttgatacaa gtttttcaat tatgttgtat ctaaaaacgc tatttgcag
//
LOCUS       DATRME010000028        21834 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRME010000028 DATRME010000000
VERSION     DATRME010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21834
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..147
                     /locus_tag="VBC23_001058"
     CDS             <1..147
                     /locus_tag="VBC23_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6843013.1"
                     /translation="KGYISSDTVMVVRKNGKIFDYVLPGEPVRLWEVATEEKVEEVLM
                     ELDK"
     gene            360..1052
                     /locus_tag="VBC23_001059"
     CDS             360..1052
                     /locus_tag="VBC23_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6843014.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            1049..1801
                     /locus_tag="VBC23_001060"
     CDS             1049..1801
                     /locus_tag="VBC23_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6843015.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            1798..2373
                     /locus_tag="VBC23_001061"
     CDS             1798..2373
                     /locus_tag="VBC23_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO6843016.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            2468..3550
                     /gene="hrcA"
                     /locus_tag="VBC23_001062"
     CDS             2468..3550
                     /gene="hrcA"
                     /locus_tag="VBC23_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6843017.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            3553..4125
                     /gene="grpE"
                     /locus_tag="VBC23_001063"
     CDS             3553..4125
                     /gene="grpE"
                     /locus_tag="VBC23_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6843018.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            4306..6135
                     /gene="dnaK"
                     /locus_tag="VBC23_001064"
     CDS             4306..6135
                     /gene="dnaK"
                     /locus_tag="VBC23_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6843019.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            6424..7539
                     /gene="dnaJ"
                     /locus_tag="VBC23_001065"
     CDS             6424..7539
                     /gene="dnaJ"
                     /locus_tag="VBC23_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6843020.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            7657..8904
                     /locus_tag="VBC23_001066"
     CDS             7657..8904
                     /locus_tag="VBC23_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6843021.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            8975..9751
                     /gene="truA"
                     /locus_tag="VBC23_001067"
     CDS             8975..9751
                     /gene="truA"
                     /locus_tag="VBC23_001067"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6843022.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            9714..10472
                     /locus_tag="VBC23_001068"
     CDS             9714..10472
                     /locus_tag="VBC23_001068"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6843023.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            10482..10946
                     /locus_tag="VBC23_001069"
     CDS             10482..10946
                     /locus_tag="VBC23_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6843024.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            10943..11512
                     /locus_tag="VBC23_001070"
     CDS             10943..11512
                     /locus_tag="VBC23_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6843025.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(11549..12391)
                     /locus_tag="VBC23_001071"
     CDS             complement(11549..12391)
                     /locus_tag="VBC23_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6843026.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            12548..13831
                     /locus_tag="VBC23_001072"
     CDS             12548..13831
                     /locus_tag="VBC23_001072"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6843027.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            14020..14604
                     /locus_tag="VBC23_001073"
     CDS             14020..14604
                     /locus_tag="VBC23_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6843028.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEEEEV"
     gene            14877..16481
                     /locus_tag="VBC23_001074"
     CDS             14877..16481
                     /locus_tag="VBC23_001074"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6843029.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            16590..17516
                     /locus_tag="VBC23_001075"
     CDS             16590..17516
                     /locus_tag="VBC23_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6843030.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            17563..17648
                     /locus_tag="VBC23_001076"
     tRNA            17563..17648
                     /locus_tag="VBC23_001076"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17597..17599,aa:Leu,seq:aag)
     gene            17711..18157
                     /locus_tag="VBC23_001077"
     CDS             17711..18157
                     /locus_tag="VBC23_001077"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6843031.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            18319..19683
                     /gene="radA"
                     /locus_tag="VBC23_001078"
     CDS             18319..19683
                     /gene="radA"
                     /locus_tag="VBC23_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6843032.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            19819..20316
                     /locus_tag="VBC23_001079"
     CDS             19819..20316
                     /locus_tag="VBC23_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6843033.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            20470..21789
                     /locus_tag="VBC23_001080"
     CDS             20470..21789
                     /locus_tag="VBC23_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6843034.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
BASE COUNT         7176 a         3362 c         4632 g         6664 t
ORIGIN      
        1 aaaggatata tcagcagtga tacagtgatg gttgtgcgta agaacggaaa gatatttgat
       61 tatgtgttac ctggtgagcc tgtaagattg tgggaagttg cgacagagga aaaagtggaa
      121 gaagtgttga tggaattaga taaataaaaa aagacttttc caagtccaaa aatcagatgc
      181 tccccagcgc aaaaattact acgttattta ctacgttgtt tttaaaatca gtgtaattga
      241 ccagataatg taaggttgta aaatgttgat ttatcaagtg attgggcaat gatagcaaat
      301 gataccatat taaggttctt ttttgttata atataataaa atgcttgtga ggtgtcagta
      361 tgagtaaagt tcgtttatat atcgctcgtc atggtaaaac aatgtttaat acaattggtc
      421 gtgctcaggg gtggagtgat actcctttaa ctacttttgg agaactcggg attaaagagc
      481 ttggttgggg actaaaggct tcaaatatta gttttaaaga ggctttttct agtgatagtg
      541 gacgtacttt acaaactatg gaaattattc tcagggaagt gcaacaagaa aatattccct
      601 atacgagaga taaacgaatc cgcgagtggt gctttggtag tttggatgga ggatatgatg
      661 gcgacctttt taatggcgtt ctgcctcgag tttcaaacgg agatatgagc catttgacac
      721 acgaagaaat tgctaatttg atttgtcaag ttgatacggc tggctgggca gaaccgtggg
      781 caatattgag taaccgtatt ctgtcaggct tcactgctat agctaaaaaa atagaggata
      841 ttgggggagg taatgcgatt gttgtcagtc atgggatgac tattgcgact tttttatggt
      901 taatcgacca tagtacccct cgtagtttag gcttagataa tggtagtgtc tcagtcgttg
      961 attttgaaga tggaacattt tctattcaat ctattgggga tatgtcctat cgagaaaaag
     1021 gaagagaaat tttagaaaaa acattgcaat gaaaaaaaat aaaattatcc gattcagttt
     1081 agttggtgtt ctacttgcga tactatgctt tagtcttttt gctttattga agcctaacag
     1141 tcaacaatca tcatctcaaa agttgaggaa tgaggatata aaaaagacat cctctcaaaa
     1201 aagaaataag aaattacgat taccagctgt atcatcaaaa gattggaact tgattttggt
     1261 caatcgtgac cataaacatg aagaattaag tccagatgtg gtgcctgttg aaaatattta
     1321 tttggataaa cgtattacga agcaagctac tcagttttta gaggctgcta gagcaattga
     1381 ttcacgagaa catttaattt cgggttatcg tagtgttgcc tatcaggaga agttgttcaa
     1441 ttcttatgtt actcaagaga tgactagtaa ccctaatttg acgaggggac aagcagaaaa
     1501 gttggtaaaa acttactctc agcctgcagg tgctagtgaa caccagactg gattagcgat
     1561 ggatatgagt actgtagatt ctttgaatga gagcgatcct agagtagtca gtcagttgaa
     1621 aaagatagct ccacaatatg gttttgtctt acggtttccg gatggtaaaa cagcagaaac
     1681 aggggtaggt tatgaagatt ggcattaccg ctatgttggg gtagagtctg caaaatatat
     1741 ggtcaaacat catttaacat tagaagaata cataacttta ttaaaggaga ataaccaatg
     1801 aggaaacgtt tttccttgct aaattttatt gttgttactt ttattttctt tttctttatt
     1861 ctttttccgc ttttaaacca taagggaaaa gtagatgcta attctaggca gagtgttacc
     1921 tacaccaaag aagaatttat acaaaaaatt gtgccagatg cgcaagatct aggaaagtcg
     1981 tacggtattc gtccttcatt tattattgca caggcggctt tggattctga tttcggagag
     2041 aaaatattag ctaataagta tcataatctg tttggtttgc ttgcagaacc aggaacgccc
     2101 tcaattacct taaatgatag tagtacagga aaaaaacagg aaaagcaatt tactcattat
     2161 aaatcttgga agtattcaat gtatgattac cttgctcata taaaatctgg agcgacaggc
     2221 aaaaaagatt catatactat aatggtgtct gttaaaaatc caaaaacttt agtgcaaaaa
     2281 ttacaagata gtggttttga taatgacaaa aagtacgcta aaaaaatgac ggaaatcatt
     2341 gatttgtatg atttaacaag atatgataag tgatattagc actctttgaa taagagtgct
     2401 aatttttgat gttttttcct tgactatctt ttaaaaagga gtataataaa atcatagtta
     2461 gcagtcgatg tgtaagagtg ctaatttcag aaagaccgag aggggtgatg tttttgtgat
     2521 tacacaacgt caaaatgata ttttgaattt aatcgttgag ttatttacac aaactcatga
     2581 accagtaggg tctaaagctt tacagagaac gattgattct agtagcgcca ctattcgtaa
     2641 tgatatggca aaactagaga agttggggtt attggaaaaa gcacacactt ctagtggacg
     2701 gatgccgagt ccagctggtt ttaagtattt tgtagagcac tcccttagac tagatagtat
     2761 tgatgagcaa gatatttatc atgttataaa agcttttgac tttgaagcat tcaaattaga
     2821 agacatgctt caaaaagcta gtcatatttt atcggagatg acgggctata catccgttat
     2881 tttagatgtg gagccagctc gtcaacgttt gacagggttt gatgttgttc agcttagcaa
     2941 ccatgatgct ctagcagtca tgactttaga tgaatctaag ccagtgacag tgcagtttgc
     3001 tattccaaga aactttttga cacgtgactt gatagcattt aaagctatcg tagaggagcg
     3061 cttgctggat ggtagtgtca tggatattca ttataagttg aggacagaaa ttcctcagat
     3121 cgttcagaag tattttgtaa ctacggataa tgttttacaa ctatttgatt atgttttttc
     3181 agagctgttt ttggaaacgg tatttgtagc tggcaaggta aattctttaa catattcaga
     3241 tctatcaacg tatcagtttt tggataatga gcagcaagta gcaatttctt tgaggcaagg
     3301 tctgaaagag ggtgagatgg cttctgtcca agtggcagat agtcaagaag ccgcacttgc
     3361 agatgtgtca gttttaactc acaaattttt gattccttac cgtggatttg gactcctgag
     3421 tttgattggt cctattgata tggattatcg taggtctgtt agtttggtaa acattatcgg
     3481 aaaggtattg gctgctaaat taggtgatta ttatcgatac ctaaattcta accattatga
     3541 agttcactga taatggcagt attcaataag ttatttaaaa ggaggcactc agtgtcagaa
     3601 gaaattaaaa aagacgattt acaggaagaa gtggaagcca ctgaaactga agagatagtt
     3661 gaggaagtta ttgaagaaat tcctgaaaaa tcagagttag aactcgcaaa cgagcgtgct
     3721 gatgagtttg aaaataaata cttgcgtgct catgcagaaa tgcagaatat acagcgtcgc
     3781 tctagtgaag aacgtcaaca acttcaacgc tatcgttctc aggatctggc aaaagcaatc
     3841 ttaccatcgc ttgacaatct agaacgtgcc ttggctgttg aagggttgac agatgatgtt
     3901 aaaaaaggtt tagaaatgac tcgtgatagc cttattcaag cactaaagga agaaggcgta
     3961 gaagaagtag aagtcgactc atttgaccac aactttcata tggcagttca gaccctccca
     4021 gccgatgatg aacatccagc cgatagcatt gcagaagtct ttcaaaaggg atataaattg
     4081 cacgagcgtc tgctaagacc agcgatggta gttgtctata actaaggtaa atgagttttc
     4141 gtttttgtcc gtaatgacag taaactagat agcaagttag aagctattca gcttgctgat
     4201 taaactatag tgattgctta gaattggaag taaaataatt cgagtgctta ctaagataaa
     4261 ttgaaataaa aagtaataaa gtattataaa ataagaggta ttaacatgtc taaaattatt
     4321 ggtattgact taggtacaac aaactcagca gtagcagttc ttgaagggac tgaatcaaaa
     4381 atcattgcta acccagaagg caatcgtaca actccttcag tagtatcatt caaaaatggt
     4441 gagattatcg tgggtgatgc tgcaaaacgt caagcggtaa caaatccaga tactgttatc
     4501 tctatcaaat caaagatggg aacttctgaa aaagtttctg caaatggtaa agaatatact
     4561 cctcaagaaa tttcagcaat gattcttcaa taccttaaag gttatgctga agactatctt
     4621 ggagaaaaag tagaaaaagc agttattact gttccagctt acttcaacga tgcacaacgt
     4681 caggcaacta aagacgctgg taaaattgca ggtcttgaag tagaacgtat cgttaacgaa
     4741 ccaacagcag ccgcacttgc ttatggtatg gacaagactg acaaggatga aaaaatctta
     4801 gtttttgacc ttggtggtgg tacatttgac gtatcaatcc ttgaattagg tgatggtgtc
     4861 ttcgacgttc ttgcaacagc aggtgataac aaacttggtg gtgacgactt tgaccagaaa
     4921 attattgatt tcttggtaga agaattcaag aaagaaaatg gtattgatct ttctcaagac
     4981 aaaatggctc ttcaacgctt gaaagatgct gctgaaaaag ctaaaaaaga cctttcaggt
     5041 gtaactcaaa ctcaaatttc attaccgttc atcactgctg gttctgctgg tcctcttcac
     5101 ttggagatga gcttatcacg tgctaaattt gacgatctca ctcgtgacct tgttgaacgt
     5161 acgaaaactc cagttcgtca agctctttca gatgcaggct tgtcattgtc agaaattgat
     5221 gaagttatcc tcgttggtgg atcaacacgt atcccagcag ttgttgaagc tgtaaaagct
     5281 gaaactggta aagaaccaaa taaatctgtt aaccctgatg aagtggttgc catgggtgct
     5341 gctatccaag gtggtgttat cactggggat gtgaaagacg ttgtacttct tgacgtaaca
     5401 ccattgtcac ttggtattga aacaatgggt ggtgtcttca ctaaattgat cgaccgcaac
     5461 acaactatcc caacatctaa atcacaagtc ttctcaacag cagcagacaa ccaaccagcc
     5521 gttgatatcc atgttcttca aggtgaacgc ccaatggcag cagataacaa aacactcggt
     5581 cgcttccaat tgactgatat cccagctgca cctcgtggaa tcccacaaat tgaagtaaca
     5641 tttgatatcg ataaaaatgg tattgtatct gttaaagcta aagatctcgg tactcaaaaa
     5701 gaacaacaca ttgttatcca atctaattca ggattaactg atgaagaaat tgataaaatg
     5761 atgaaagatg ctgaagcaaa tgctgaagca gatgcaaaac gtaaagaaga agttgatctt
     5821 aaaaatgaag ttgaccaagc catctttgca acagaaaaaa ctattaaaga aactgaaggt
     5881 aaaggctttg atacagaacg cgatgcagcg caatcagcac ttgatgagtt gaaaaaagct
     5941 caagaatcag gtaaccttga cgacatgaaa gctaaacttg aagctcttaa cgaaaaagca
     6001 caagctcttg cagttaaact ttacgaacaa gcagctgcag cacaacaagc agctcaaggg
     6061 gctgaaggtg cacaatcagc tgattcatca agcaagggtg atgatgttgt agatggcgaa
     6121 ttcactgaga aataattatt aatattgttc agattcattt gaatataagc atgaaaacta
     6181 tactagcata gtaaagttct tcgtgatagg ggttgctcaa taatctagat aagtttcaga
     6241 ttacataagc taatttcgct atcactaaat aaaaacatat taataataaa taggcggggc
     6301 gcctcgctcc gtctgtttta ttaagtgtca tatatatgtt aactatttag agctgtaact
     6361 gggcaagaat aattgttaat ctcttcaagt gtagtatatg aacaaaatat aaaggattag
     6421 ataatgaaca atacagaatt ttatgatcgt cttggcgttt caaaagatgc ttctcaggac
     6481 gaaataaaaa aagcttaccg taggatgtct aagaaatacc atccagatat taataaggaa
     6541 gcgggagcag aggaaaaata taaagaggtt caagaagctt atgaaacctt gagtgataca
     6601 caaaaacgtg ctgcctatga tcaatatggc gctgctgggg caaatggcgg ttttggtggc
     6661 ttcgatggtg gtggctttgg tggttttgaa gacatcttct caagcttttt tggtggagga
     6721 gggatgcgca atcctaatgc accacgtcaa ggagatgacc tacaatatcg tgttaattta
     6781 agttttgaag aagctatctt tggagctgaa aaagaagtat cttataatcg ggagtcatca
     6841 tgtcatactt gttcaggaag tggagctaag ccaggtacta gccctgtgac ttgtcaaaag
     6901 tgtcatggtt cgggtgtgat taatgttgat acacaaacac ctctaggaac aatgcgtcgc
     6961 caagtgactt gtgatgtttg tcagggtagt ggtcaagaaa tcaaagagaa atgtccaaca
     7021 tgtcatggaa ctggtcatga gaagaagacg cataaagtat cagttaaaat acctgcagga
     7081 gttgaaactg ggcaacaaat tcgtttaaca ggacaaggag aagctggctt caatggtggt
     7141 ccttatggtg atctctttgt tattattaac gttctcccaa gccaacagtt tgagcgtaat
     7201 ggatcaacaa tttattatac tttgaatatt ggctttgtcc aagctgcttt gggtgataca
     7261 attgatatac ctactgtcca tggagcggta gaaatgtcaa ttcctgctgg tactcagaca
     7321 ggaaaaacgt tccgtcttcg cggaaaaggt gcccctaaac ttcgaggggg tggacaaggt
     7381 gatcagcatg ttactgttaa catcgtaact cctactaaat tgaatgatgc tcaaaaagag
     7441 gcattgcacg cttttgcaga ggcaagtgga gataagatgg tacatccgaa gaagaaaggt
     7501 ttctttgata aagtcaaaga tgctttagat gttgattaaa aaataaaagt ctagaatcga
     7561 aagtattcta gacttttttg tagttattgc tagagggatt gcttgaacct aaattttaca
     7621 gtacaatgaa tctaatattg tagggggaca atttttatgg taaccaaagt tgaagagatt
     7681 cgctcatatt tgatagcttc tatacaaaat ggtaagttgg ctccaggaga tcgcctacca
     7741 tctataagac agttagctaa tcaattttcc tgtaacaaag atacagtgca acgagttttg
     7801 atggaattgc gttttgataa ttatatctat gcaaagccta ggtcaggcta ttacgtcttt
     7861 gattctcatc aagaggaagt tgaagaaggg gttagtttac caaactctga gattgcaaat
     7921 atagcttatg atgattttag attgtgtttg aatgagaccc ttattggtag ggaagattac
     7981 cttttcaatt attactatcg tcaagaaggt cttcttgatt taagtaaagc agtggctaaa
     8041 ttaatggaag aaacaggggt ctatgttccc cttgatgata ttgttattac ggctggtact
     8101 caacaggcat tatttatttt gacacaggtt acctttccaa atcgaaaatc tcgagtttta
     8161 atagaagaac cgacttatcc tcgtatgatt gaactaatca aaacacaaaa tttaccctat
     8221 gaaactattc ctcgaggtac tcatggaatt gattttcagc gcttagagga gattttccag
     8281 acacaatcaa ttaagttttt ttatgttata cctcgcatgc ataatccttt gggaacatcc
     8341 tataatccgg tagagatgaa aagattaata gagatggcag agaagtatga tgtttatatt
     8401 gtggaagatg actatatgtc tgattttgca agtcagtcac cattacatta ttatgatact
     8461 cacgggcgtg ttatttatct aaaatctttt tcaaaggcta ttttccctgc tttaagatta
     8521 gctgcgattt gtttaccaca agctttaaaa tcaacattta tggcttacaa gaagttgatg
     8581 gattatgata ctaatctgat tttacaaaaa gcattagcgc tttatattga aaatggcctt
     8641 tatgctaaga atagtcaata tttgaaatat cgttatcaga aagaccttgc aaattcaaaa
     8701 tctattttag ctgatcaccc taatctaccc ccatatagtt tacatcacga tagtgtatta
     8761 tttgattgtt cgaaactcga taacttcaaa atattacggc aatacggcga tactttggaa
     8821 aattattttt gtcaaaaatc gcatcaatct ctcttacaag taaaaaatga ttcctgctta
     8881 aagcagttct tgggatcgtt gtagtataat tgtaggggga cagtttaaaa tagatattat
     8941 agttttcata aatgaaaggt ggtaaaataa acccatgaca aggtataagg cacagatttc
     9001 ttatgatggt tcagcttttt caggcttcca aagacaacca aattgtagga cagttcaaga
     9061 agaaattgag agaactctta agcgcctaaa tagcgggaac gatgtgataa tctatggtgc
     9121 tggtcgcaca gatgctggtg ttcatgctta tggacaagtt attcattttg atttaccaca
     9181 ggcgcgtgat gtagaaaaat tacgctttgg tttagataca cagtgtccag acgatattga
     9241 tattgttaaa gtagagcaag tgtctgatga ttttcattgt cgatatgata aacacattaa
     9301 aacttatgag tttttagtag atatcggtag gccgaaaaat cctatgatga gaaactatgc
     9361 aacacattac ccttatcctg tcattattga gcttatgcaa gaagctatta aagatttagt
     9421 aggcacccat gactttacag gctttactgc ttcaggtacg agtgtagaaa ataaagtacg
     9481 tactattttt gatgcgaaaa ttcagtttga agcgtctaaa aatcttttaa tttttacctt
     9541 tacaggaaat ggttttttgt ataaacaggt gcgtaatatg gttggtaccc tgctaaaaat
     9601 tggtaatgga cgcatgccta ttagccaaat caaaacaatt ttacaggcta aaaatcggga
     9661 tttagcaggt ccaactgcgg caggaaatgg tttatatttg aaggagatta tttatgaaga
     9721 cgaggaatgt tttagcaatt tcaggaaatg atattttctc tggcggcggc ttgcatgcag
     9781 atttagcaac ttacgttgtt aataaattac atggttttgt ggcagtaaca tgcttaacag
     9841 caatgtcaga taaagggttt gaagtcattc ctatagaggc atctatacta aagcagcaac
     9901 tagaaagttt aaaagatgta gagtttgggt ctattaaact aggattgctt cccaatgtag
     9961 aaacagcaca agttgttttg gaatttgtaa aatcaaaaca agaatgtcca gtagtcttag
    10021 acccagtctt agtttgtaaa gaaaatcatg atttagaagt tagccaatta cgtgagcagc
    10081 taattgcgtt tttcccatat gctgatgtga ttactcctaa tttagtagag actcaattat
    10141 tgacgggtct ttcaattgaa aacttagatc aaatgaaaat tgctgctgag aaactttacg
    10201 atatgggggc taaacatgtt gttatcaaag ggggcaaccg tttaaatgct gaagaggcta
    10261 cagaccttta ctatgatggt gaacgttttg aaacctatgt ttttcctgta gtagatgcca
    10321 ataatacagg tgctggatgt acatttgcgt caagtattgc cagccaatta gctatgggta
    10381 aaaatgtaga agatgcagtc aaaatgtcta aaggtttcgt ctatcaagct attaaagcat
    10441 cggataagta cggagttgtc caacattttt aggagatttt aatgaggaaa gagaaaacta
    10501 gtcaattggt tcaattggca attgtaacag ctatatctat tgtattgggg atgtttataa
    10561 gtattccgac accaacaggt tttttgacat tactagatgc tggaatattc tttgctgcct
    10621 tttactttgg caaaaaagaa ggggctgtgg taggtgcctt agctggtttt ttaattgatt
    10681 tattgaaagg ttatcccaat tggatgttct ttagtttatt gattcatggg gcgcaagggt
    10741 atttagcggg tttaccaggt agaagacgtc ttttaggtct tattagtgca actcttgtta
    10801 tggtattagg ttatgctata gctagtggat tgatgtatgg ttggggagct gttttaccag
    10861 atattcctgg caatattatg caaaatatgg ttggtatggt cgttggtttt gctcttaata
    10921 agtctctaga aagagtgaag aaatgatact agattataat aagttaaagc aggaaactaa
    10981 agctattgta gttgatatta ttgagcgtag tgctcttaaa aaaggtcaaa tttttgtact
    11041 aggtctatca tctagtgagg tttctggtgg tttaattgga aaaaattcta gttctgaaat
    11101 tggtgaaatt atcgttgaag ttatcctcaa agagttacat tcacgcggca tctatttagc
    11161 agttcaaggt tgtgaacatg ttaatagggc gttggttgtt gaagcagaat tggctgaacg
    11221 tcaacagttg gaagtcgtta atgttgttcc taatctacat gctggcggta gtggtcaggt
    11281 tgcagctttt aagttgatga catcaccagt tgaggttgaa gaaattgtgg cacatgctgg
    11341 aattgatatt ggagatactt ctattggaat gcatatcaag agagtacaag ttcccttgat
    11401 tccaattagt cgtgagttgg gaggggcaca cgttacagca ctagctagtc gtcctaaatt
    11461 aattggcggt gctagagcag gatatacgag cgatccaatc cgtaaatttt aaaataaacc
    11521 tctgctatgc agaggtttat tgtttatctc aacgagagtg gttattggag aaattgtatg
    11581 ttggtaagtc aataccttct tcaagaatag ctttttgata aagtttgtaa aattcagcat
    11641 agatgtcaaa ctgagcaccg ttttgcgtga agatattaat acgataaaca aattgaccat
    11701 tagcattggt tgtaggacca aaaacagtag gctctccaac gatagcagga tgttttgaaa
    11761 catactcttc attgattttt gttacaatat cagaaatctg atcaaggttt gtatgaacaa
    11821 ataaaggaat atcaatttga gcacgcatat tgctacgaga cttattgctg acaattgtaa
    11881 tattacgatt aggaatgaag tgtaaggttc catcgaaatc atgaatttgt gtggtgcgta
    11941 tcccaacatt agtaacagtg cctgatacag caccaacatt aataatatca ccaacatcaa
    12001 attggttttc tagcagaata aaaaagccat tgacaacatc tgataagaag ccttgagcac
    12061 caagtccaat agcaacccca gcaataccag ccccagctaa taaactagaa attggaacgc
    12121 ccaatatact taaaatccaa tagaatagaa agaaataaag ggtatagttt aaaacattgt
    12181 gagaaagttt agctaatgtt ttttgacgag ctactttttg tctagaaaag gcgagtgagc
    12241 gattaacagt cttttcgaaa agataattga tgacttgcct aacgataacg aatgcaatga
    12301 ttaataataa gattgatatt agtttggtga agaaagtaaa taaaacttcc tctacattaa
    12361 gatggtcaat aaatttttct aaagtaatca tagtaatagt ctagtaaaaa aaaagccata
    12421 gagcaagtat tttaaaagct tatcgttaag cagtttaaag cttctttttt gcataaaaac
    12481 tttgtaaaac gttttaaatt atggtaaact aaactgtatc taaataattt taaggagaaa
    12541 tgacttaatg tctacatcat ttgaaaataa agctacaaac cgtggtatta tcacatttac
    12601 aattagtcaa gatgagatca agccagctct tgatcaagca ttcaacaaag ttaaaaaaga
    12661 tttgaacgtt ccaggattcc gtaaaggaca tatgcctcgt acagttttca accaaaaatt
    12721 tggtgaagaa gcactctacg aaaatgcatt gaatttagtg ttgccaaagg cttacgaagc
    12781 agctgttgca gaacttggtt tagacgttgt tgctcaacca aaaattgatg ttgtatcaat
    12841 ggaaaaaggt caagattgga agttgacagc tgaagttgtt acaaaaccag aagttaaact
    12901 tggggattac aaagaccttt cagtagaagt tgatgcttca aaagaagttt cagatgaaga
    12961 agttgatgct aaagttgaac gtgagcgtaa caaccttgca gaattgactg ttaaagatgg
    13021 tgaagcagca caaggtgata cagttgttat tgactttgta ggttcagttg atggtgttga
    13081 atttgatggt ggtaaaggag ataacttctc acttgaactt ggttcaggtc aatttatccc
    13141 aggatttgaa gaacaattag taggttctaa agctggtcaa actgtagacg ttaatgtaac
    13201 attcccagaa gattatcaag cagaagatct tgctggtaaa gatgctaaat ttgttacaac
    13261 aattcatgaa gtaaaaacta aagaagttcc agcacttgat gatgaacttg caaaagatat
    13321 tgatgatgaa gttgaaacac ttgatgagtt gaaagcgaaa tatcgcaaag agctagaatc
    13381 agcaaaagaa attgcatttg atgatgcagt tgaaggtgta gcaatcgaat tagctgttgc
    13441 caatgcagaa attgttgaat taccagaaga aatggtccat gatgaagtac atcgtgctat
    13501 gaatgagttc atgggtaata tgcaacgtca aggaatttca ccagaaatgt acttccaatt
    13561 aacaggtaca actgaagaag accttcataa gcaataccaa gcagatgctg acaaacgcgt
    13621 taaaactaac cttgttattg aagcaattgc agctgcagaa ggctttgaag caacagatga
    13681 agaaatcgaa aaagaaatca cagatcttgc ttcagaatat aatatggaag ctgaccaagt
    13741 acgcggtcta ctttcagcag atatgcttaa acatgatatt gcaatgaaaa aagcggttga
    13801 cgttatcact agctcagcta ctgtaaaata attgcttatt aaattttgaa aagattgggc
    13861 ttcccagtct ttttatttgt gattattctt ttgacgaata gctaaaaata gcgtaaaata
    13921 gtaaatagcg aaaatttgaa aatatgactt atctattttc tatctataaa gaatattgct
    13981 aaacaaaatg atagtcttac acaattaagg agaataactt tggaattaga agtatttgct
    14041 gggcaagaaa aaagtgaact ttcgatgatt gaagttgctc gcgctatttt agagcaacgt
    14101 ggtcgtgata acgaaatgta cttcagcgat ttggtaaatg atattcaaac ctaccttgga
    14161 aaatctgact cagcaatccg tgagtcattg ccatttttct attcagattt gaatacagac
    14221 ggaagtttta ttccacttgg cgaaaacaaa tggggacttc gttcatggta tgctatcgat
    14281 gaaattgacg aggaaatcat cactcttgaa gaagatgaag atggcgctcc aaaacgtaag
    14341 aagaaacgtg tcaatgcttt catggatggt gatgaagatg ctattgatta caatgatgat
    14401 gatccagaag atgaagattt cacagaagaa acaccaagtt tagaatatga tgaagaaaac
    14461 cctgacgatg aaaaatcaga ggtagaatcg tatgattctg agattaatga aatcattcct
    14521 gatgaagatt tagatgagga tgtagaaatc aacgaagaag aggaagaaga ggaagaagag
    14581 gaagaagagg aagaagaggt ttagatgggg attttgtatt tgacaaatag tcagatatag
    14641 tttaaactat tattcgggca cctcttatga ggtcagataa agctccctag tgataggggg
    14701 ctattttgtt tttgggaaag atacagaatg gttccagata gttttctgag taggcccttg
    14761 atcagatagt ttctactagt tttgactaga gaagctggta ttatttatca atcatgtgtt
    14821 ttgggtttta cccgtaaact aactgttaga ttaacattta atttaaggag tttttaatga
    14881 caaagtatat ttttgttaca ggtggtgtgg tctcttctat tggtaagggg attgttgcag
    14941 caagtcttgg gcgcttacta aaaaaccgtg ggctaaaggt gacaattcaa aaatttgatc
    15001 catatattaa tattgaccca gggacaatga gcccttatca acatggggaa gtttatgtta
    15061 cagatgatgg tgcagaaact gaccttgacc ttggacacta cgagcgtttt attgatatta
    15121 acttaaacaa gtattcaaat gttacaacgg gtaagattta tagtgaagtt cttaaaaaag
    15181 aacgccgtgg agaatacctt ggtgcaactg ttcaagttat cccacacgtt acggatgctt
    15241 tgaaagaaaa aatcaaacgt gctgcgacaa ctacagattc agatgttatt attactgaag
    15301 ttggcggtac tgttggagat attgagagcc ttccgttttt agaagcactt cgtcaaatga
    15361 aggcagatgt tggttctgat aatgttatgt acattcatac aacgttatta ccatacctaa
    15421 aggctgccgg cgaaatgaaa acaaaaccga ctcaacattc tgttaaagag ttacgtggcc
    15481 taggtatcca acctaatatg cttgttatcc gtacagagca accagcaggt caaagtatta
    15541 agaataaatt ggcacaattt tgtgatgttg caccagaggc tgttatagag tcccttgatg
    15601 ttgaccatat ttatcaaatt ccacttaaca tgcaagctca aaatatggat caaattgttt
    15661 gtgatcactt gaagcttgag acacctgctg ctgatatgac agaatggtca gcaatggttg
    15721 ataaagtaat gaaccttgag aaaaaagtta aaattgcatt ggttggtaaa tatgttgaat
    15781 tacctgatgc ttacctttca gttgtggagg cccttaagca ctcagggtac gtcaacgatg
    15841 ttgctattga cctaaaatgg gtaaatgctg ctgaagtgac agaagataat attaaagaac
    15901 ttgtcggaga tgcagatggt atcatcgttc ctggtggatt tggccaaaga ggtagtgagg
    15961 gtaaaattga agcaattcgc tatgcacgtg aaaatgatgt tccaatgctt ggtgtttgtc
    16021 tcggaatgca gctaacttgt gtcgaatttg cgcgcaatgt tttaaacctt catggagcta
    16081 attcagcaga gcttgaccct aaaacaccgt tcccaatcat tgatatcatg cgtgatcaaa
    16141 tcgatattga agatatgggt ggtacacttc gccttggtct atatccatgt aaattgaaag
    16201 caggttcaag agctgcggca gcttataata atcaagaagt tgtccaacgt cgtcaccgtc
    16261 accgttatga atttaataca aaattccgtg agcaatttga ggctgctgga tttgtcttct
    16321 caggggtatc accggataat cgtttgatgg aagttgttga gttaccagag aaaaaattct
    16381 ttgttgcagc acaataccat ccagaactac aatcacgtcc taaccatgct gaagagcttt
    16441 acacagcttt tgtaacagca gcagttgaaa atatgaaata actttatcgc ctcccagtat
    16501 taggaggcga ttttattttt ttagatagtt tcactatcaa agtattatta gctcttttct
    16561 ttttctaaaa ataaggtata ataggttgca tgaaaaagat tcgattatca aagtttatta
    16621 aaatgattgt tgttattttg tttttaatta gtgtagcagc tagtttttat tttttccacg
    16681 ttgcccaagt tcgagatgat aaatccttta tttcaaatgg tcaacgtaag cctggaaact
    16741 ctttatatgc ttatgataaa tcctttgata agctattaaa gcaaaaaata gaaatgacaa
    16801 accaaaatat aaagcaagtt gcttggtatg ttcctgctgc taagaaaact cataagacag
    16861 ttgttgtcgt tcatggtttt gcgaatagca aagagaatat gaaggcatat ggttggctgt
    16921 ttcataagtt aggatacaat gttcttatgc ctgacaacat tgcacatggt gaaagtcatg
    16981 ggcagttgat aggctatggc tggaacgacc gcgagaacat tatcaaatgg acagaaatga
    17041 tagtggataa gaatccatca agccaaatta ctttatttgg tgtttcaatg ggtggagcaa
    17101 cagtcatgat ggctagtggt gaaaaattac ctagtcaggt tgttaatatc attgaagatt
    17161 gtggttattc tagtgtttgg gatgaattaa aatttcaggc taaagagatg tatggtttac
    17221 cagccttccc actcttatat gaagtttcaa caatttctaa aatcagagca ggtttttcgt
    17281 atggacaagc aagtagtgtc gaacaattga aaaagaataa tttaccagcc ctctttattc
    17341 atggtgataa ggataatttt gttccaacaa gtatggttta tgacaactat aaagctacag
    17401 caggtaagaa agagctttat attgtaaaag gggcaaaaca tgcgaaatct tttgaaacag
    17461 agccagaaaa atatgagaaa cgtatctcta gttttttgaa aaaatatgaa aaataatatt
    17521 gacaacaata tgataagttg atataattta ttatgttgtt aagcggggat ggcggaattg
    17581 gcagacgcgc aagactaagg atcttgtgac cgcttttggt cgtgagggtt caagtccctc
    17641 tctccgcaga ctagtcgagt tcatttggaa ctcgactttt tgcatattta agataaagat
    17701 ggagtatcac atgtcaaaag tacgtggctt tgaattagtt agccaattta gcaataaaga
    17761 gttattacca aaacgagaaa cagctcacgc agctggctat gatttaaaag tagctaaaaa
    17821 aacggttatt gaacctggtg aaattacatt ggtcccgacc ggtattaaag cacacatgca
    17881 accaggggag gtcctgtacc tttatgatcg ttcatcgaat ccacgtaaaa aagggattgt
    17941 cctaataaat tcagttggtg ttattgatgg tgactactat aataaccaag tcaatgaagg
    18001 acatattttt gcacagatgc aaaatattac tgatcaggct gttattttag aagaagggga
    18061 acggattgtt caggctgttt ttgccccatt cttattggca gatgacgatc aggctacagg
    18121 aatgcgtaca ggtggttttg gtagtactgg aaaataaaat tattttacga ataatgttag
    18181 aaaaggctag acttacttct tagccttttt tgatgagagt aaacttatat tttctctaac
    18241 ttggtgtgat ataatgagtg tactagttat ttggtaccaa gctatcatat taatttagaa
    18301 gttataaagg aggcctgagt ggctaagaaa aaatcagtct tcacctgtca agagtgtggt
    18361 taccaatcac ctaagtattt gggacgttgt ccaaattgct cagcctggtc ttcatttgta
    18421 gaagaggttg aagttcaaga agttaaaaat gcacgagtta gtttgaacgg tgaaaagtcc
    18481 cgaccgacga agttaaaaga tgtttcctca attaactatt ctagaactaa gactgatatg
    18541 gatgaattta accgtgttct aggaggcggt gttgtaccag gaagtttggt tctgattgga
    18601 ggagaccctg ggataggtaa atctacttta ttactacaag tatcaactca gctagctaat
    18661 aaaggtacag tattatatgt ctcaggtgag gaatctgcag aacagattaa gttacgaagt
    18721 gagcgtctag gcgatatcga taatgaattt tacctttatg ccgagactaa tatgcaaagt
    18781 attcggtcgg agattgagaa gattaaacca gattttctta ttattgattc tatacaaact
    18841 attatgagtc ccgaagtatc aagtgtccaa ggctctgtta gccaagttcg cgaagtgacc
    18901 gctgagttga tgcagttagc taaaactaat aatattgcta cttttattgt aggtcatgtg
    18961 actaaggagg gaacattagc aggccctcga atgctagaac atatggtgga tacggttctt
    19021 tattttgaag gggagcgtca tcacacgttc cgtattttac gggcagtcaa aaaccgtttt
    19081 ggttcgacca atgaaatagg tatctttgaa atgcagtcgg gtggtctagt tgaagttttg
    19141 aatccaagtc aagtttttct tgaagaacgt ttagacggtg caacaggatc tgctattgtt
    19201 gttactatgg aaggaacaag gcctatttta gcagaggttc aagctttagt gactccaact
    19261 gtttttggaa atgctaagcg cacaaccaca ggtcttgact ttaatcgtgt cagtcttatt
    19321 atggcagttc tagaaaaacg ttgtggttta ttgcttcaaa atcaagatgc ctatttaaaa
    19381 tcagcgggtg gtgttaaatt ggatgaacca gcaattgatt tagctgttgc tgtagctatt
    19441 gcttccagtt acaaagaaaa acccacaaat ccacaagaat catttattgg agaaattgga
    19501 ctcactggtg agattcgtcg cgtgacacgt attgagcaac gtattaatga ggctagtaaa
    19561 ttgggcttta ctaaaattta cgcaccaaag aattctctag ctgggattga aatacctaaa
    19621 ggcattgatg ttattggtgt gacaactgtt agtcaggtct taaaagcagt gttttcaaca
    19681 taatgattag attcggttca gccgaatctt ttttggtggc gagagacatt tcgtgaaaaa
    19741 taatgggcaa aatgataata taaaagggtt ggtatttagt aaatatcgaa tgatgttaca
    19801 ctaacaaagg agatctctat gaccacttat tttgataact ttttaaaaac caatcaagcc
    19861 tatgctgact tacatggaac agctcatcta cctatcaagc caaagacaaa ggttgctatt
    19921 gtaacctgta tggattctcg tttacacgtt gctcaagctc tagggctagc tttaggagat
    19981 gctcatattt tacgtaatgc tggtggacga gttacagccg atgttctacg ttctttagtt
    20041 atttcgcaac aacaattagg aacacgagaa atcgttgttc tacaccatac agattgtgga
    20101 gcacagacgt ttactaacga agcttttgct gctcaattac agagagattt gggtgttgat
    20161 atgcatggac atgattttct tccttttaat gatattgaag aaagcgttcg tgaagatgtg
    20221 gcaaaattac atgcttctcc attaatccca aatgatgttg ttatttctgg tgctatttat
    20281 gatgtagata ctggtcgtat ggtggaagtt aaatagaaaa aatcctgtcc ttagcgatgg
    20341 gtttttttat tttagaaaac tagatttaaa ggtaacagtt aggcttatca tgccttatgc
    20401 tggaattcag acagaggaaa catcttttgc tatactgaaa ctatattata aaagcggagg
    20461 aaatttaaaa tgaaaaaata tgatgtcatt gttcttggtt ttggtaaagc aggtaaaaca
    20521 ttagcagcaa aattagcaac acaaggtaaa agcgttgcaa tggtcgaaga agacgataaa
    20581 atgtatgggg gaacatgtat caacattgga tgtattccga caaaaacgct tttagtatca
    20641 gctagcaaaa atcacgattt tcaagaagct atgactacta gaaatgaagt tacaagtcgt
    20701 ttacgtgcta aaaattttgc tatgttggat aataaagaca ctgtggatgt ttataatgct
    20761 aaagctcgct ttatatcaaa taaagtggtc gaattaacag gaggagctga caaacaagaa
    20821 cttacagctg atgttattat tatcaatact ggtgctaagt cagtacaatt accaattcca
    20881 ggattagctg attctcaaca tgtttatgat tcaacggcaa tccaagaatt ggctcatctt
    20941 cctaaacggt taggtatcat tggaggcggt aatattgggc ttgaatttgc gactttatat
    21001 tccgaattag ggagcaaagt gacagttatt gatagtcaat ctcgtatctt tgcgcgtgag
    21061 gaagaggaat tatcagagat ggctcaagat tatttagaag agatggggat atcttttaaa
    21121 ttatcagccg atatcaaatc tgtgcaaaat gaagatgaaa acgttgttat tagctttgag
    21181 gatgaaaaat taagctttga tgcagtatta tatgcaacag gacgtaagcc aaatacagaa
    21241 ggtttagcac ttgaaaatac agatattaaa ttgacagaac gtggagctat tgctgttgat
    21301 gaatattgcc aaacttctgt tgagaatatt tttgcagttg gtgatgttaa tggcggccct
    21361 caattcacat atatttcttt agatgactca cgtattgtct taaattattt gaatggtgat
    21421 aaggattatt ctctaaaaaa tcgaggagca gtaccgactt caacctttac aaatcctcct
    21481 ttagcaacgg taggtctaga tgaaaagacc gctaaggaaa aaggatatca agttaaatct
    21541 aatagccttc ttgtttctgc catgccacgt gcacatgtta ataatgacct tagaggtatt
    21601 tttaaagttg tggtagatac tgagacaaat cttattttgg gagctcgctt atttggtgca
    21661 gaatcccatg aattaattaa tatcatcaca atggcaatgg ataataaaat cccatatact
    21721 tatttccaaa aacaaatttt cactcaccca acaatggtag agaattttaa tgatttgttt
    21781 aacttttaag aatattttta aaaagtaagg caattttgct ttactttttt tatc
//
LOCUS       DATRME010000029        21493 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRME010000029 DATRME010000000
VERSION     DATRME010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21493
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            76..726
                     /locus_tag="VBC23_001081"
     CDS             76..726
                     /locus_tag="VBC23_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843035.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            737..1567
                     /locus_tag="VBC23_001082"
     CDS             737..1567
                     /locus_tag="VBC23_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6843036.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            1580..4225
                     /locus_tag="VBC23_001083"
     CDS             1580..4225
                     /locus_tag="VBC23_001083"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6843037.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            4363..4665
                     /gene="gatC"
                     /locus_tag="VBC23_001084"
     CDS             4363..4665
                     /gene="gatC"
                     /locus_tag="VBC23_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6843038.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            4665..6131
                     /gene="gatA"
                     /locus_tag="VBC23_001085"
     CDS             4665..6131
                     /gene="gatA"
                     /locus_tag="VBC23_001085"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6843039.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            6131..7573
                     /gene="gatB"
                     /locus_tag="VBC23_001086"
     CDS             6131..7573
                     /gene="gatB"
                     /locus_tag="VBC23_001086"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6843040.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            7703..8617
                     /locus_tag="VBC23_001087"
     CDS             7703..8617
                     /locus_tag="VBC23_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6843041.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            8701..9261
                     /locus_tag="VBC23_001088"
     CDS             8701..9261
                     /locus_tag="VBC23_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6843042.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            9261..10379
                     /gene="yqeH"
                     /locus_tag="VBC23_001089"
     CDS             9261..10379
                     /gene="yqeH"
                     /locus_tag="VBC23_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6843043.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            10472..10789
                     /gene="yhbY"
                     /locus_tag="VBC23_001090"
     CDS             10472..10789
                     /gene="yhbY"
                     /locus_tag="VBC23_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6843044.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            10919..11551
                     /locus_tag="VBC23_001091"
     CDS             10919..11551
                     /locus_tag="VBC23_001091"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6843045.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            11548..12135
                     /gene="yqeK"
                     /locus_tag="VBC23_001092"
     CDS             11548..12135
                     /gene="yqeK"
                     /locus_tag="VBC23_001092"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6843046.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            12198..12719
                     /locus_tag="VBC23_001093"
     CDS             12198..12719
                     /locus_tag="VBC23_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6843047.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            12721..13077
                     /gene="rsfS"
                     /locus_tag="VBC23_001094"
     CDS             12721..13077
                     /gene="rsfS"
                     /locus_tag="VBC23_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6843048.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            13143..13877
                     /locus_tag="VBC23_001095"
     CDS             13143..13877
                     /locus_tag="VBC23_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843049.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            13900..14151
                     /locus_tag="VBC23_001096"
     CDS             13900..14151
                     /locus_tag="VBC23_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843050.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            14148..15257
                     /locus_tag="VBC23_001097"
     CDS             14148..15257
                     /locus_tag="VBC23_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6843051.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            15350..16064
                     /locus_tag="VBC23_001098"
                     /pseudo
     CDS             15350..16064
                     /locus_tag="VBC23_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            16203..16607
                     /locus_tag="VBC23_001099"
     CDS             16203..16607
                     /locus_tag="VBC23_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6843052.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(16872..17585)
                     /locus_tag="VBC23_001100"
     CDS             complement(16872..17585)
                     /locus_tag="VBC23_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6843053.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGVISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(17595..17969)
                     /locus_tag="VBC23_001101"
     CDS             complement(17595..17969)
                     /locus_tag="VBC23_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6843054.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            complement(17969..18547)
                     /gene="dhaL"
                     /locus_tag="VBC23_001102"
     CDS             complement(17969..18547)
                     /gene="dhaL"
                     /locus_tag="VBC23_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6843055.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            complement(18585..19574)
                     /gene="dhaK"
                     /locus_tag="VBC23_001103"
     CDS             complement(18585..19574)
                     /gene="dhaK"
                     /locus_tag="VBC23_001103"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6843056.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            19748..20284
                     /gene="dhaS"
                     /locus_tag="VBC23_001104"
     CDS             19748..20284
                     /gene="dhaS"
                     /locus_tag="VBC23_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6843057.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     NDFSSYFESYCEQLVDKLLSDYSKSNFNQKERVTFINYHSKALSYFIKYELINNSKEE
                     LFNRKVIEKIIRTSIENY"
     gene            20294..21280
                     /gene="dhaQ"
                     /locus_tag="VBC23_001105"
     CDS             20294..21280
                     /gene="dhaQ"
                     /locus_tag="VBC23_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6843058.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
BASE COUNT         7122 a         3387 c         4313 g         6671 t
ORIGIN      
        1 taaaatagta tcttttttta cttgatttca gtaaaaaaag atatataata taacatataa
       61 aataactagt atacaatata taaagaaggt gatcttattc aattaaatca aaggcaagaa
      121 agaattattg atattatcaa acagtttcag cccattactg gtgagctaat agctgagaaa
      181 ttaggagtaa caagagcagc actaaggtct gatttggcgg ttttaagtat gctaggtatc
      241 atagatgcaa aacctaaggt tggttatttt tatttaggac agtatcatgc ttcaataggg
      301 acaagtcatt ttgaaaagat gacagtttca gaaattatgg ggatccctct gacagttcat
      361 caaaaagatt cagtttatga tgttattgta catattttta tggaagatgc tggttgtgct
      421 tttatcttgg atgatgatga ttttctctgt ggagtcgtgt cacgtaaaga tttactaaaa
      481 accagtattg gcggaggaga tctttctaaa atgccaatag gaatggtgat gacacgtatg
      541 ccacacgtga caactgtttt agaaaatgaa agtctttttg cggcagctga taaattagtg
      601 agcagaaaag tggatagtct ccctgtcgtt cgtcatgata agcaatatcc cgaaaaattt
      661 aaagtgatcg ggaaattatc taaaacaatc ctcgctagtt tgtttttaga aataagagat
      721 tattaggagg aaactgatgg aagatcaact cactatattt attatttcag attctttagg
      781 ggaaacagcc aaagctattg cgaaagcatg tttgtcacaa tttccaggac atgatgattg
      841 gcattttcaa cgtttttcct atattaacag tcaggaacgc ttagaacaag tttttgaaga
      901 ggctagccag aagacagtat ttatgatgtt tagtttagtt gatgttgctt tggcttccta
      961 tgcacagaaa cgatgtgaaa gtgaacatta tgcttatgtt gacttattaa caaatgttat
     1021 tcaaggaata tcaaggatat cgggtataga tcctttggga gaaccaggga ttttacgacg
     1081 cttagacaat gattatttca aaagagtaga atcaatagaa tttgcagtaa aatatgatga
     1141 tgggagagat ccgcgaggta ttttacaagc agatttagtt attataggaa tttcaagaac
     1201 ttctaaaaca ccattaagta tgtttttagc agataaaaat attaaagtta ttaatattcc
     1261 tttggttcca gaggttcctg ttcctaaaga attacgtatg attgattcta gaaggattat
     1321 cggactaact aattcggttg atcatcttaa tcaagtccga aaagttcgat taaagtcctt
     1381 aggtctttct agtacggcaa attatgccag tttagaacgg attttggagg agacgaggta
     1441 tgctgaagaa gttatgaaaa atttaggttg cccaatcatt aatgtatctg ataaagcaat
     1501 cgaagaaaca gcaacgatta ttttagaaat cttaaaaaca aatggacaag tagctaaaaa
     1561 tctgtaaaag gagacgctga tggaaactaa gtttgtgtat cattttgatg aaggttgtaa
     1621 ggaaatgaag gaactgctag gtggtaaggg ggctaatcta gctgaaatga cgagtattgg
     1681 gttaccggta ccacaaggtt ttactattac aacacaggct tgtaatgatt attatgataa
     1741 cgcttgccat atccgagaga gtattcttag tcaaattgat caagcaatgg cacaattgga
     1801 ggttgaacag aacaagcaat taggaagtgt tgatgatcct ttgttggtgt cagttcggtc
     1861 aggatcagtt ttttcaatgc ctggtatgat ggacacagtt ttaaatttag ggttaaatga
     1921 tcgtagtgtc caaggtttag tgaaaaagac agaagacgag cgttttgctt atgacagtta
     1981 tcgtcgtttt atccaaatgt ttgcagatgt ggttacaggc attccaaaat ataaatttga
     2041 taccatttta gatcgactta agacagataa atgttatcaa gatgacacag aactaactgg
     2101 ttcagattta aagagacttg ttgaatttta caaggaacta tatcaaaaag aagcaggaga
     2161 aaaatttccg caagatccta aaaggcagct tttgttagca attgaggcag tttttaaatc
     2221 atggaataat ccaagagcta agatttatcg taaattaaat gatattccgg agacgcttgg
     2281 gacagccgtt aatatccaag ctatggtttt tgggaatatg ggaaataaca gcggaacggg
     2341 agttgctttt acacgaaacc catcaactgg agcagcaaat ttatttggtg agtatttgat
     2401 taatgcacaa ggagaagatg ttgttgcagg tatcagaaca ccacaatcca tatcaaaatt
     2461 ggcagaacaa atgcctatta tttaccaaga gtttgtaagc gttacccaaa agttagaagc
     2521 tcattatcgc gatatgcaag atatggagtt tacaattgaa aatggtaatc tttatatgct
     2581 tcagacgaga agcggtaagc gtacagcgaa ggcagctatc aaaatagcag tagatcaagt
     2641 aaatgaaggc ctaattagta aagaagaagc tatactacgt atcgagccca aacaattaga
     2701 tcagttatta cacccttctt ttgacttgaa aagtttaaag aaagctatta ttttgacgac
     2761 aggacttcca gcttctcctg gagctgctta tgggaaagtt tatttccatg cagaagatgt
     2821 tgttaaagag atgaaaaagg gaaatccagt gctattggtc agacaagaga catcacctga
     2881 agatatcgag gggatggtta gtgcaaacgg gattataaca gcaagaggcg gaatgacttc
     2941 ccatgcggca gtagtagcca gaggaatggg gaaaccatgc gttgcaggct gtagtcaatt
     3001 attggttgat gaagtaagac gagaaatttc tattgggcat caaacgataa aagaaggtga
     3061 gatgttatca atcgatggtg ctacaggaaa tgtttatata ggacaagttc cgatggcaga
     3121 gactagcgta gatcgtgatt ttgagatatt tatgaaatgg gtagatgaaa atcgagatat
     3181 gatggtgtgt tcaaatgcag ataatccgag agatgctcaa aaggccttgg actttggagc
     3241 agaaggaata ggtctttgtc gcacggaaca tatgtttttt gacgatgaac gtattccagt
     3301 cgttagagaa atgattttag cggatgaaat attgagccga aggaaggctc tagaaaggct
     3361 tttatctttc caaagagacg atttttatca aatttttaaa gtattgaaag gtaaagcttg
     3421 tacgattcgt ttgcttgacc ctcctttaca tgaattctta ccccatgata aagagtctat
     3481 agaatctatg gcaagacaaa tggggatatc tactttagct attgaaaaac ggattcaaac
     3541 attagaagaa ttcaacccta tgttaggtca tcgtggttgt agacttgcga ttacctatcc
     3601 ggaaatttac caaatgcagg tgagagctct tgtacaaggc gctatacttg caatgaaaga
     3661 gggatatgag gcaaagccag aaattatgat tcctttagtg acagcgcatg aagaaatctc
     3721 tataatacgt gatctgattg aagagacaat tgttgaggag agtaaatcta aaaaaattaa
     3781 cttatcattc cctattggga caatgattga aacaccaaga gcttgtatga tagcggatga
     3841 tattgcaaag tttgcggatt tctttagttt tgggaccaat gatttaacac aaatgagctt
     3901 tggattttca cgagatgatg ctggaaaatt cttaggagaa tacgttgata aaggacttct
     3961 aaaaaaagac cctttccaag ttttagatca aaaaggaata ggaaggttta ttgagcaggc
     4021 agtgaggtta ggaaaagaag ttaagccaaa ccttaaaata ggtatctgtg gagagcatgg
     4081 tggagaacca tcatctattg agttctgtta ccaacagggg ttacattatg tatcgtgttc
     4141 gccgtttagg attcctattg ctaaattagc ggctgcacag gctaaaatca aacaaagcag
     4201 aaatgatata acaatagata aataaacagt aaacgcttct tatcataggt aagaagcgtt
     4261 tattatgaaa agctgggata atgtgataac attgagagtt taatatgcta taatagtaga
     4321 gactatctta aaacgaaaat gatacagaga taaggagaac tcatgaaaat ttctgaagaa
     4381 gaagtgcgtc atgttgccca tctgtcaaaa ttgcgctttt ctgatcaaga gactaaagaa
     4441 tttgcgtcaa gtttgtcaaa gattgttgat atgattgaac tcttaaatga agttgacaca
     4501 gaaggcgtac cagtaacaac gaccatggca gatcgcaaaa ctgtgatgcg tgaagatatt
     4561 gctcaaccag gccataatcg tgatgactta tttaaaaatg taccacagca tcaagattat
     4621 tatattaaag tacctgcaat tcttgaggat ggaggagatg cctaatgtcg tttaataatc
     4681 aatcaattga tcagttgcat gattttctcg ttaaaaaaga aatttcagca actgaattaa
     4741 caaaagctac attggaagat attcatgcgc gtgaacaagc ggttggatct tttattacta
     4801 tttctgatga gatggcaata gctcaagcca aagaaattga tgataaagga attgatgctg
     4861 ataatgttat gtcaggtatt cccttagcag ttaaagataa tatttcaaca aaggggattc
     4921 ttacaacagc tgcttctaaa atgctctata actatgagcc aatctttgat gctacggctg
     4981 ttgaaaagtt atatgctaaa gatatgattg tgattggtaa agcaaacatg gatgaatttg
     5041 ccatgggagg ctctacagaa acatcttatt ttaaaaagac aaataacgct tgggaccata
     5101 gtaaagttcc gggaggatca tcaggtggtt ctgcagcagc agtagcttca ggacaagtac
     5161 gtttatcatt gggctctgat acaggtggtt caattcgcca accggcttca tttaatggaa
     5221 tcgttggtat gaagccaact tacggacgtg tatcacgctt tggtttgttt gctttcggta
     5281 gttcgcttga ccagattgga ccgatgtctc aaacagtaaa agagaatgca cagcttttga
     5341 cagttatttc tgggcatgat gtaagagatt caacttcttc tgagcgtact gtaggcgatt
     5401 ttactgctaa aattggtcaa gatattcaag gaatgaaaat tgctttacca aaagaatatt
     5461 tgggagaagg aatagcgcaa ggagttaaag aaacgattat taaggccgca aaacatctgg
     5521 aaaaactagg tgcagttatt gaggaagtta gtttaccgca ttctaaatac ggtgttgctg
     5581 tttattatat tgttgcttca tcggaagctt cttcaaactt acaacgtttt gatggtattc
     5641 gttatggata ccgtacagaa aattataaaa acttggatga tatctatgtc aatacacgat
     5701 ctgaaggttt tggagacgaa gttaagcgtc gcattatgct gggaacgttc agtttatctt
     5761 ctgggtatta cgatgcttat tataaaaaag caggtcaagt tcgtagtttg attattcaag
     5821 attttgaaaa agttttcgca gattatgact tgattttagg tccaacagct ccaacaacag
     5881 cctttgactt agattcttta aatcacgatc cagttgcaat gtacctagct gatattctaa
     5941 caataccagt taatttggct ggtttaccag gtatttcaat ccctgctggt tttgatcaag
     6001 gtctaccggt tggtatgcag ttgattggtc cgaaattcag tgaagaaaca atatatcagg
     6061 ttgctgctgc cttcgaagca acgacagatt atcataaaca acaacctaaa atttttggag
     6121 gtgagaacta atgaattttg aaacagtcat tggacttgaa gtccatgttg aattaaatac
     6181 gaactctaaa attttctcac cttcttccgc tcattttggt caggaacaaa atgctaatac
     6241 aaatgtaatt gactggtctt tcccaggtgt ccttccagtc atgaataaag gagttattga
     6301 tgcaggaatc aaggcggcac ttgccttgaa tatggatatc catcaaaaca tgcattttga
     6361 tcgtaaaaat tatttctatc cagataatcc taaagcttat caaatttcac agtttgatga
     6421 gcctattggt tacaatggtt ggattgaaat cgaattagaa gatggtacac gaaaaaaaat
     6481 tcgtattgag cgtgcccacc tcgaagaaga tgcaggaaaa aatacacatg ggacagatgg
     6541 ttattcctat gttgacctta atagacaagg tgttccactg attgaaattg tatcagaagc
     6601 tgatatgcgt tctcctgagg aagcttatgc ttatttgaca gctctcaaag agattatcca
     6661 atatactgga atttctgacg ttaaaatgga agaaggttct atgcgtgtag atgccaatat
     6721 ttcacttcgt ccttatggtc aagaagagtt tggtactaaa gcagaactta agaaccttaa
     6781 ctcattcaat aatgtacgta aaggtttgat acatgaagaa aaacgtcaag cacaagtttt
     6841 acgatctggt ggtcaaattc agcaagagac tcgtcgtttt gatgaaacaa caggtgagac
     6901 tattttgatg cgtgtcaagg aaggctcatc agattatcgc tatttccctg aaccagatct
     6961 accactattt gatatctcgg atgaatggat tgatcaagtt cgtttagaat taccagaatt
     7021 tccacaggaa cgtcgtgcga aatatgtatc gtcttttggt ctatcatctt atgatgctag
     7081 ccaattaaca gctacaaaag caacttctga tttctttgaa aaagctgttg ctattggggg
     7141 tgatgcaaaa caagtttcca attggttaca aggagaagtg gcgcaatttc ttaacagtga
     7201 atcaaaatca atcgaagaaa ttggtctgac tcctgagaat ttggttgaga tgattggctt
     7261 gattgctgac ggtacaattt catccaaaat tgctaaaaaa gtttttgtgc atttagctaa
     7321 aaatggtggt tcagctgaag aattcgttaa aaaggctggt ttagttcaaa tttcagatcc
     7381 tgaagtatta atcccaatta ttcatcaagt ctttgcagat aatgaagctg ctgtgattga
     7441 ctttaagtca ggaaaacgca atgctgataa ggccttcaca ggttatctaa tgaaagcaac
     7501 aaaaggacaa gctaatccac aagttgctct gaagctgtta gcgcaagaat tagcaaaatt
     7561 aaaagaagaa tagaaatcag ccttttggct ggtttttatt cgctaattta gcacgaacac
     7621 ctagggataa ataattaaga agctaagata tttatcagat gactttaact acagtgttta
     7681 agaaataaaa atgaggggaa aaatgactaa aaaagaaaaa ggaacaatga tgaccttagc
     7741 tgccggacta gcatggggca tttctggaat tagtggtcaa tacttgatgt cgcacggagt
     7801 acacgttaac cttttgactt ctttacgatt gttgataaca ggtatctttc tcttgtcttt
     7861 agctagatca aaacaaaagg agcatttagt agcagcgtgg aaacagccaa aatttttaaa
     7921 gcaggtttta cttttttcta tatttggttt agttttaaat caatatgctt ttctcagagc
     7981 gattcattta acaaatgctg gaactgcaac ggttttgcaa tatatggcac caatacttat
     8041 tttgagtatt gtttgtatcc taaacagaca aagaccaaca tcttttgaaa taatagcaat
     8101 tgctatggca attttaggaa cctatatgat tgcaacgcat gggaagttag gaagtttagc
     8161 tatcacaccg aaaggattaa tgtgggggct tggttccgcc attacttatt ctatttatat
     8221 tttattacct gtgaaactta ttcatgaatg gggaagtacg attgttattg gttcaggaat
     8281 gtttataggt gggattttat ttagtttggt gacaaaagcg tggcaatacc ccttacaaat
     8341 taatgtgatg agtatacttg cttatattgg cattattggt attgggacta tttttgctta
     8401 cacttttttc cttaaagggg ttagtattgt aggtgctgtc aagggtagct tattagcatc
     8461 ggtagagcca gtttcttcgg tatttttgac agtacttgtt ttaggagaaa ttttttatcc
     8521 aatagattta cttgggatgc tgtttatttt tcttgctgta actctaatct cttacaaaga
     8581 tttaattgca ttgaagaaaa tgaaacgagt aagatagtat cttttggggt tgaaacccta
     8641 atttaaaggt ctttaaagac cttttttctt tactcgctat ctcttaagaa ttatggtaga
     8701 atgaatagta aaatatattt tttggagatg aaattgagta tagatgatta taggccgacc
     8761 ttcgtggttg aggcagttta tgatttaaga gcagaagatt tattacgtca tggcattcgt
     8821 gcggttttag ttgatttaga taatacactc attgcttgga ataatccaga tggtactgct
     8881 gaggtgcgtg catggttaga tgagatgaca acagcggata tttcagttgt agtagtgtct
     8941 aataacaatc atgctcgcgt agaacgtgca gtatcacgtt ttggtgtgga ttttgtaagt
     9001 cgtgcgatga aaccatttac tcgtggtata aacatggcaa ttgagcgtta tggctttgat
     9061 cgtgacgagg tgattatggt tggagatcaa ttgatgacag atattcgtgc tagttatcgc
     9121 gcaggaataa agtccgtatt ggtaaaacca attgttaaat ctgatgcttg gaataccaag
     9181 ttcaatcgct tacgtgaacg tcgcgtttgg aaaaaaatag aagagaatta cggtaaaatc
     9241 gtatatcaaa aaggaattta atggaagagt tattttgtat tggctgcgga gccaggattc
     9301 aaactgaaaa taaagatgct gctggctata cacctcgtgc agcacttgaa aaaggtttag
     9361 agacaggaga attatactgt cagcgttgtt tcagattgcg ccactataat gagattactg
     9421 acgttcatat cactgatgat gaatttttga aattactaca tgaggttgga gatagtgatg
     9481 cccttgtggt gaatgtcatt gatatttttg atttcaatgg ttcaattatt cctggtctta
     9541 gtcgtttcgt tgcaggaaac gatgttcttt tagtaggaaa taaaaaagat attcttccca
     9601 aatcggttaa agatggaaaa gtaacacaat ggttaactga gagagctcat gaagaggggc
     9661 ttcgtcctgt tgatgtcatc ttgacaagtg cccaaaatca tcatgctatt aaggatttga
     9721 ttgatacgat tgaaaagtat cgtcatggtc gagatgttta tgttgttggg gttactaatg
     9781 ttggaaagtc aactcttatt aacgctatta ttcgtgaaat tactggtagt cgtgatgtta
     9841 ttacgacctc acgtttccca ggtacaacgt tagataaaat agaaatacct cttgatgatg
     9901 gtagctatat ttttgatacc ccgggtatta ttcatcgtca tcaaatggct cattatttga
     9961 cagcaaaaaa tcttaaatat gttagtccta aaaaagaaat aaaacccaaa acttatcagc
    10021 ttaattcaga acaaacatta ttcttggctg gcctagcacg ttttgatttt atttctggtc
    10081 aaaaacaagg ttttacggca tattttgata ataacctgaa tttacaccgc actaaactag
    10141 ttggagcaga tgaattttac actaaacatg ttggaaaatt attgacacca ccaacaggta
    10201 aggaagtttc agattttcct aaattagttc gtcatgaatt tacaatcaaa gataaaatgg
    10261 atattgttta ttcgggtctt ggttggatac gtgtgaaatc agaagcagaa aatccagtag
    10321 tagtagctgc ttgggctcca gaaggcgtgg cagttgtttt acgcaaagct ttaatttaat
    10381 aactaaatta accagcaaca tatgacggtt tttcatctaa tttagctgat gatacagtca
    10441 taatcctata atagataaaa aggaaactaa tatgttaaca tctaaacaac gtgctttctt
    10501 aaaatcagaa gcacattcta tgaaacccat tattcaaatt gggaaaaatg gtttaaatga
    10561 tcaaatcaaa acaagcgtac gtaatgcact cgatgcacgt gagctaatca aagtaaccct
    10621 tttacaaaat accgatgaag atatccacga tgtcgctgaa gttctggaag atgaaattgg
    10681 ctgcgatact gtccttaaaa taggacgtat cttgattctt tataaagagt cagcgcgcaa
    10741 agaaaatcgt aaaatttcag ttaaagtaaa agcagtaaaa tcaaactaaa ctacaataga
    10801 ttaagaaata gagtatagaa ctaaaaaacg atttacctaa gttacacttc cgtatgatag
    10861 ctgatcatga taatcagtgt ttgttttttt agagatacta cttgttcatg gaggaattat
    10921 ggcactcgaa ttgctaacac catttacaaa agttgaatta gaagagaaaa agcgagatac
    10981 taaccgaaaa caaattggaa ttatgggagg aaattttaac cctgttcata acgcccacct
    11041 tgttgttgca gatcaagtac gtcagcaatt gtgtttagac caagttctgt tgatgcctga
    11101 atttcagcca ccacatattg ataaaaaaga gacaattgat gaacagcatc gtctcaagat
    11161 gcttgaatta gcaatagaag gcattgatgg tttatctatt gaaccaattg aaattgaacg
    11221 taaatgtatc agctatacat acgatacgat gaagcttcta attgaaaaaa atccagatgt
    11281 tgattattat ttcattattg gggcagatat ggtggaatat ctacctaagt ggcatcgtat
    11341 tgatgaactt gttaaaatgg tgcagtttgt tggtgttcaa cgtccaaaat ataaagctgg
    11401 tacatcatat cccgttattt gggtggatct gccattgatg gatatctcat caagtatgat
    11461 tcgtcaattt attaaaagta atcgtcagcc caactattta ctgcctagag aggtattaga
    11521 ttatatcagg aaagaaggtc tttacaaatg acctacaaag attacacagg tttagatcgg
    11581 actgaacttt tgagtaaagt gcgtcatatg atgtccgaca aacgttttaa tcatgtgtta
    11641 ggagtagagc gtgctgcaat agagttagca gaacggtatg gttatgacaa ggaaaaagcg
    11701 ggtctagctg ctttattaca tgattatgct aaggaactat ctgatgatga atttcttaga
    11761 ttaattgata aataccaact agatcccgac ctaaaaaaat ggggtaataa tatttggcat
    11821 ggtcttgttg gcatttataa aatacaagag gatctagcta tcaaagatca agatatttta
    11881 gctgctattg caaaacatac tgttggttca gcccaaatgt ccacattgga taaaatcgtt
    11941 tatgtagcag attacattga acacaatcga gattttccag gagttgaaga agctcgtgag
    12001 ttagcgaaag ttgaccttaa taaagcagta gcttatgaga ctgctagaac agttgcgttt
    12061 ttagcaagta aggctcagcc tatctatcct aaaacgattg aaacctataa tgcttatata
    12121 ccttatctcg actaattctt ttattgaata atggtcatga taggtttggc gatgcagtgg
    12181 tcattttgga ggaaagtatg acttgtgcac ttttactaat tgatatccaa caaggtatta
    12241 tggataagaa accaaagcat ttaacaaatt ttgcagagct tttagatgat ttacttttaa
    12301 gtgccaaagg ttctaattgt gaagtgatat ggataaggca tcatgataag gaattgcctc
    12361 aaggttctcc acagtgggaa atttgggaac agcgacatct tgtgacgcat cacaaaataa
    12421 ttgataaaac ttacaatagt tgttttaaag atactcattt acatgattac ttgcaaagta
    12481 agcatattag tcaattaatc atgatgggac ttcaaacaga atactgtttt gatacatcag
    12541 taaaagttgc ttttgaatat ggttatgata tctttattcc acaaggtgga cacctaacat
    12601 ttgatacacc aactttaagt ggggattcta ttaaaaaaca ttacgagaat atttggcatc
    12661 atcgttttgc gacaatggta gcaaaagata gcttattgaa attaataaac gaggaataaa
    12721 atgacagaaa aagacttact acagctagtc gttaaggctg cggatgaaaa acgagcagaa
    12781 gatattgtaa ttttagattt acagcctgta acgagtgttg cagattactt tgtgattatg
    12841 agtgcaagta atagtaggca gttagaagca atcgcagaca atatccgtga acaagtaaaa
    12901 ggaaatggtg gggatgctag ccaccttgaa ggcgatagta aagctggttg ggtacttctt
    12961 gacctcaata gcgtcgtggt tcatatcttc tctgaagatg agagacagca ctataacctt
    13021 gaaaaacttt ggcatgaagc accattgctt gatgctgaag tctttatgac agaataaagc
    13081 taagcagtct tgggactgct ttttgagaga tgtaatatca agaaagattt gagaactatt
    13141 atatggcaac gtatgaaact tttgcagctg tttatgatgc tgtcatggat gatactttat
    13201 atgataaatg gactgatttt tcacttcgtc attttccaaa aggtaagaaa aaacttttgg
    13261 aattagcatg tggtacggga attcagtctg ttcgatttgc acaagctggt tatgctgtta
    13321 caggacttga tcttagtggc gatatgctga aacttgccaa aaaacgtgca acttctgctc
    13381 accaaagtat ccagtttatt gaaggaaata tgttagattt atctaatgtt ggtaaatatg
    13441 atctcatcac gtgctattca gactctattt gttatatgca agatgaagtc gaggttggtg
    13501 atgtttttat cgaagtctat aaggccttag aagaaaatgg cgtctttatt tttgatgttc
    13561 attcgactta tcaaacagat aaagtatttc ctggttattc ttatcatgaa aatgcagatg
    13621 actttgctat ggtgtgggat acttatgaag atgatgcacc tcattctatt gtccatgaat
    13681 taacattttt tgttcaagaa gaagatggtc gatttacacg tcatgatgaa gttcatgaag
    13741 agcgtactta tgatattttg acttatgata ttttacttga acaggcaggt tttaaagatg
    13801 ttaaggtgta tgcagatttt gaagataaaa aaccaactgc aacttctgct cgctggtttt
    13861 ttgtagctca taaataagta ataatttgga ggtaaaaaga tgataatatt cttatacgct
    13921 tgtctgatga tatttgggat actttatatt attgaacgtc gggtaagtca gaaaaagtct
    13981 aaagttattt tggaattatt gatgaaccct aaacttcatt tggatttatt atcggcatta
    14041 aaaccatttg aaaaagaaac agatcgtctc atataccttc gtaaaactta tcacctagat
    14101 attattgatg ctgttacttt aaataatcaa ttgaaagaga aagaagtatg acagttacag
    14161 gaattgttgc cgaatttaat ccctttcata atgggcataa gtacctttta gaacaagctc
    14221 aaggcataaa agtaatagcc atgtcgggta attttatgca acgtggtgag ccggcgattg
    14281 tagataaatg gacccgtgcc cagatggcac tagaaaatgg tgctgattta gtgattgaac
    14341 tcccttttct ggtttcggta cagtcagcgg attatttcgc tagtggagct gtgtctatcc
    14401 tggcacgatt aggtgtggat aacttatgct tcggtaccga agaaatgctt gattatgccc
    14461 gtattgggga tatttatgtg aataaaaaag aggagatgga agcttttttg aaaaaacaat
    14521 cggattctct ttcctatcca caaaaaatgc aagctatgtg gcaagaattt gcagggatta
    14581 cattttcggg acaaacccca aatcacattt taggcttagc ctacaccaaa gcagctagcc
    14641 aaaatggtat tcgattaaat ccgatacaac gtcaaggagc tggttatcat tcatcagaaa
    14701 aaacagagat atttgcttct gcaacaagtc ttcgtaaaca tcaatctgat cgtttctttg
    14761 tagaaaaagg tatgcctaac agtgacttgt ttttgaatag tccacaagta gtttggcaag
    14821 attattttag tttactcaaa tatcagataa tgacgcattc tgatttgaca caaatttatc
    14881 aagtgaatga agagatagcc aatcgtataa aaagtcaaat tcgttatgtg gagactgtgg
    14941 atgagttagt tgataaagtc gcaactaaac ggtataccaa agcaagaatt cgacgtttat
    15001 taacctatat cttgataaat gctgtggaaa gtcctatacc aaatgctatt catgtcttag
    15061 gttttacaca aaaaggtcaa cagcatttaa agtctgtgaa gaagtctgtg gatatcgtga
    15121 cgaggattgg ttcacaaacg tgggatagtt tgacgcaacg agcagattct gtttatcaga
    15181 tgggaaatgc taatatagca gagcaaacat ggggacgtat ccctttttat caacctgtta
    15241 gtcaaagtga cctttgattt aagctttata aattaatcat cacaaaataa tatttttccc
    15301 ttgaccctta cttaacgtta tacattatac tctttacaga gaggttatta tgaaaacagt
    15361 taaagaaata agtcacattt ctggtattag tgtgcgcaca cttcattatt atgatgaaat
    15421 agatttgtta tctccttcat ttgtaggaga gaatggttat cgttactatg atgatgaatc
    15481 actgattaag ttacaagaaa ttttgttatt taaggaactg gaatttcctc ttaaaaaaat
    15541 aaaagagata atggaaagtc caaattatga tcgtaatcaa gcacttcttg atcaaattag
    15601 gtggctggaa ctaaagaaac agcgactaga agaagtaatt gaacatgcaa agtctatcca
    15661 gaggggtaaa aatatgtcag attttacagc ttataatcaa gaagaattag aagtatttca
    15721 agaggaagct agaacacgct gggggggata cagatagtta caaagaattt gaaaatagcc
    15781 atagtaagaa tgatttttca atgatttcac aagctatgag tcagattttt aaagactttg
    15841 gacagttgaa agaactttct ccgacagatg agaaagttca aaaacaagtt cagattttac
    15901 aagattatat cacagctcag ttttacaact gcactaacga tttattagca agtcttgaaa
    15961 taatgtacat ctaagatgag cgtttccaaa agtctattga taattgggga ggccagggaa
    16021 cagcattatt tgtttctaaa gctatagata gctattgtca ttaggctata ttttaggttt
    16081 gcaatgaaat gataggaatc aaggaacagt agatattatt aaaaatgtca gactcttatc
    16141 taaatttttg acagtaagga gcaaaaagag tagactgtag gcatagaaag gtaggtatcg
    16201 ttatgaaagg atttcatggg aatatcgaaa aattgacact aggaaataca aatttccgcc
    16261 aagtcctcta taccgcagaa cattgtcaac tcgttttaat gacactgcct gttggtggag
    16321 aaattggtag cgagattcat gcagaaaatg atcagttttt ccgttttgaa gctggtcatg
    16381 gtaaggttgt cattgatggt aatgaatatg aagttgcaga tggagacgct ataatagttc
    16441 cggcaggtgc tgaacataac gtaattaaca cttctgaaac tgagatgtta aaactctata
    16501 caatttacag tccagctcat cacaaagatg gtattatccg tgccacaaga gaagaagctg
    16561 aagaaaatga agaagatttt gatggagtta ctactgaaac taaataatac tagactaaaa
    16621 agcgctatgt caatagcgct ttttagctta atagtagtca tttcttggag aatataaata
    16681 gtaatttagg aagtaatgtt aataaccgag cttttaagga aacgttaagt aagggcggtt
    16741 atactataaa catcctaaac ttttttcata aaatctccag aaagaacact aagtattttg
    16801 gtgttttttc tattttgaaa gcatactgca actatgaaaa accagaaagt attaactttc
    16861 tggtttttta gttaaactaa agtcaaagca atattgtata agatagcccc aaggacaccg
    16921 ccgataattg gacctacaac tggaatccaa gcgtatgacc aatctgagtt acccttattt
    16981 tcaataggta agatagcatg cataagacga ggccctaggt cacgtgcagg gttaatagca
    17041 taaccagttg ttggaccaag agagaatcct actgcgaaaa taactatacc aacgataatt
    17101 ggatcaagac ctgctgcaac tttactagga ccaattgcca taattgtaat cacaagaata
    17161 gcagtaccga taatttcacc aaggagattc gatggtgtat ggcggatagc aggtccggtt
    17221 gagaagctgg caaggataag tccactatct tttgtttctt tccaatgagg ataatagtgt
    17281 aaccataaaa tagttgccgc aaccattgct cctaacatct gtgcaatgat aaaggggata
    17341 acttgtcccc aagaaattac tccaatagaa gccattgcta atgtgactgc tggatttaga
    17401 tgagcgggac tgaataatcc agaaacataa acagcaacgg tacaagcaat tccccagcca
    17461 aaaacaatag cagtccatcc agttccttct tctttggttt tactgagcac atttcctgca
    17521 actacgccat ccccaagaag aaccaaaata aatgttccta aaaattcccc aagaaattga
    17581 gtcataatat ctccttattt attgatagtt aattcagcta attgtgattg aatactatcc
    17641 aggtcagcac ctgcctgtag aagagcagca gcggtgtaag ctccctccac tactgggaca
    17701 gagttaatga taatattttt ttctgaaaaa tcagctacta actctaaatt catttttgct
    17761 gaacctaaat caaagaaagc taataacgtc tttttatcat tttgctctac aatctgttga
    17821 acttgatcaa aagaagtacc aatctcacca tcttctgttc cacccacgta agttattgag
    17881 acatcctttg ctacctctga aataagatca acaactcctt gagcaatatt tttagagtga
    17941 gaaacaacaa taataccaat atcagacatt agacacctcc aacttctagt aatgctttaa
    18001 ataataatcc tgaagaaaat gatcctggat caatgtgtcc tactgagcgt tctccaacat
    18061 aagaagcgcg tccttttgtt gctttcatcc cttttgtagc atctactaag gaatcgatac
    18121 ggtcttcagt caattcattt ttttcaatcg cttcaataac accgtgccaa acatcaacca
    18181 ttgttttttc attaagttca gctttaccac gtttttgaat catttcaagt cctgcgccaa
    18241 gtgcctctga aatggtactt tcactctgtt cagcttttgt aattcccata aatgcagaac
    18301 catagagtgg tccagatgct ccgcctactt tgcttaaaag ttgcatggac acggttttga
    18361 aaacatcggc agcactacta aattcattgt tatcaaggtt ttcaataaca gcagtcatac
    18421 cacgcgccat attgccacca tggtcgccat cacctattgg agtatctagt tcggataaat
    18481 agtccttgtt ggattgaatt ttttggttaa aagtgtgcat ccattctatt gcagttttta
    18541 cttccataag agttccttcc ttttgttcta attaacagga atgattacca agcaatggtt
    18601 gtaacgtcac tattaagtgc ttctaaccac tcttgatttt ccagtttaat caaggttagt
    18661 gataaaccag ccatatcaat tgaagtcata tagttaccaa gttttttgta attcacttca
    18721 atgcctttat cctcaaggag tttggcaaca tcgttagcaa agacatattg ttccataagt
    18781 ggggttgcac ccataccgtt aataaggaca ccatattttt cgccagattt agcatcgaac
    18841 gattcaatta acttatcaac taattctgtc gctaaagcct ttgaaggttg cattttttct
    18901 ttacgataac ctggttcacc atgaataccg ataccaaatt caatttcatc ttctgcaaga
    18961 acaaatcctg gcttgcctac ttcaggaaca gtagccccgc ttagtgcaag tccaacagtg
    19021 tgaatgtttg gtactaattc atcagcaata gctttgattt cctgaagtga tttgccatga
    19081 cgagcagcat gtcctagaat tttatggacg agtatcgtac cagctacacc acgttttcct
    19141 tgtgtgtaaa gactgtcttc gactgcaata tcatcatcaa cgaccacact tgcaacttct
    19201 atgccttcca tttcagccat atcttgtgcc atttcaaagt tcatgatatc ccctgaataa
    19261 tttttgataa ccatgaaaac tccagccccc tcatcagctt ctttaattgc ttcaagaact
    19321 tggtcaggag ttggagaagt aaatactgca ccacacacag ctgcagataa cattccttcg
    19381 cctacgaaac cagcatgtga aggctcatgt ccacttcctc cgccagaaat gagagccact
    19441 tttcctgact tctcttcatt gcgagcaatg atatcaaaac cttctatgcg atgtactaaa
    19501 tcattatgaa catatgctaa tccatctagc atttcagtaa caacatcagt tggttgattg
    19561 agaatttttt tcatttggaa actcctttta tgtttaattc tcttttatta taaaatttgt
    19621 aagccttttc tggaatggac agattttctt gtttgtccgc tttgttaggg ttttcaataa
    19681 tcataaatca tgttacacta aaccataata aatcatcagc aattcaatat aaaataaagg
    19741 agaaattatg acttcttcgc ttattactaa gaaaaaaata gccaagtcat ttaaacgttt
    19801 atttatttcg caagcttttg acaaaatatc ggtgagtgat attatggaag atgcaggtat
    19861 tagacgtcaa acattttata atcactttgt tgataaatat gctttattag aatggatttt
    19921 ccaaacagaa ttaagcgaac aagttacgga taatttagac tatatctcag gttttcagtt
    19981 gttatcagag ttgttgactt tttttaaaat gaatcaagaa ttctatatta aattatttca
    20041 aattgaagat caaaatgatt tttcttcgta ttttgagtct tactgtgagc agttagtaga
    20101 taagcttctt tctgattata gtaaatctaa ctttaatcag aaagaaagag ttacatttat
    20161 taattatcat agtaaagccc tctcttattt tataaaatat gagttaataa ataactcaaa
    20221 agaagaatta tttaatagga aagtcattga aaaaattatc agaacatcaa tagaaaatta
    20281 ttaggacaag gatatgagaa aaacagaaca tcataaaatg gaatatgttt ttgaaacaat
    20341 aaaagcaatc actaaaacta atcctaaagt agagcaaata aaagatttgc ccgctattca
    20401 ttatttaggg caatccgagc aagtaattcc gattttatca ggaggaggca gcggacatga
    20461 gccggctcac tttggatatg ttggtgaggg aatgctatcg gcagcgatta gtggtcctat
    20521 ttttgtacca ccatgtgcta gtgatattct agagactatt agatttatca atcgaggcaa
    20581 aggtgtcttt gttattatca aaaattttga agctgatttg gaagaatttt cccaagcgat
    20641 tgaacaggct cgtcaagaag gcattccaat caaatacatc gtttctcatg atgacatctc
    20701 tgttgaaaca tcaaatttta aaattcgtca tcgaggcgtt gctggtactg tgttgctaca
    20761 taaaattatt ggacaagctg ctttagaagg agcaagctta gatgaattag aacagctagg
    20821 actatctttg acaacttcaa tggctacttt aggtgtagca agtaagtcag ccactatttt
    20881 aggtcagcat caacctgttt ttgatttaga agaaggttac atctcatttg gaatcggtat
    20941 tcatggtgag ccaggatacc ggacaatgcc tttcgtgtcc atggaacatt tggcaaacga
    21001 actagttaac aaattgaaaa tgaaattacg ttggcaagat ggagaagcat tcattctatt
    21061 gataaataat cttggaggaa gttctaaaat ggaggaatta ctttttacaa atgctgtcat
    21121 ggaatttctg gctttagacg atttacaatt accttttatt aaaacaggcc acctaattac
    21181 aagtttagat atggctggtt tatcagttac cttgtgtcgt gtaaaggata gtagatggat
    21241 tgattactta aaacataaga cagatgctag agcttggtga tagaagtaag tggtaagagt
    21301 ttttagaata gaaaatactt taaggagaac agagaaatct gttctctttt tgttcgctta
    21361 aagatcatag aaaggtgaag agaacatgga aggtattgaa ataatattag atatagaaac
    21421 gttaggaatt tttcaagtca tagatcatgg tatctctcca gatgatagag aaacatatga
    21481 acaattaact ttg
//
LOCUS       DATRME010000030        20876 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRME010000030 DATRME010000000
VERSION     DATRME010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20876
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(22..633)
                     /locus_tag="VBC23_001106"
     CDS             complement(22..633)
                     /locus_tag="VBC23_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6843059.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            726..1075
                     /gene="ssrA"
                     /locus_tag="VBC23_001107"
     tmRNA           726..1075
                     /gene="ssrA"
                     /locus_tag="VBC23_001107"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1344..1982
                     /locus_tag="VBC23_001108"
     CDS             1344..1982
                     /locus_tag="VBC23_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6843060.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(2100..3203)
                     /locus_tag="VBC23_001109"
     CDS             complement(2100..3203)
                     /locus_tag="VBC23_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6843061.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLE
                     FEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
     gene            complement(3203..5995)
                     /locus_tag="VBC23_001110"
     CDS             complement(3203..5995)
                     /locus_tag="VBC23_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO6843062.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIVI
                     GLDEVRKLFSSRKNKR"
     gene            complement(6144..6581)
                     /gene="msrB"
                     /locus_tag="VBC23_001111"
     CDS             complement(6144..6581)
                     /gene="msrB"
                     /locus_tag="VBC23_001111"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6843063.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            complement(6581..7045)
                     /locus_tag="VBC23_001112"
     CDS             complement(6581..7045)
                     /locus_tag="VBC23_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843064.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            complement(7054..7713)
                     /locus_tag="VBC23_001113"
     CDS             complement(7054..7713)
                     /locus_tag="VBC23_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6843065.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            complement(7813..10446)
                     /locus_tag="VBC23_001114"
     CDS             complement(7813..10446)
                     /locus_tag="VBC23_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6843066.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNS
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            complement(10692..12524)
                     /gene="lepA"
                     /locus_tag="VBC23_001115"
     CDS             complement(10692..12524)
                     /gene="lepA"
                     /locus_tag="VBC23_001115"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6843067.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGFTIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            complement(12660..13076)
                     /gene="ndk"
                     /locus_tag="VBC23_001116"
     CDS             complement(12660..13076)
                     /gene="ndk"
                     /locus_tag="VBC23_001116"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6843068.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            complement(13386..13646)
                     /locus_tag="VBC23_001117"
     CDS             complement(13386..13646)
                     /locus_tag="VBC23_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843069.1"
                     /translation="MLGHRCGVLVFCYTEINFQRMWGKAVIRLIQDVIQNLVGQANEI
                     TPVYQFDWETYILATKKYERHLEVCLLVENSNYFSDSKRMCR"
     repeat_region   13699..14592
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=13699..13734
                     /rpt_unit_seq="gttttggaaccattcgaaacagcacagctctaaaac"
     gene            complement(14693..15358)
                     /gene="csn2"
                     /locus_tag="VBC23_001118"
     CDS             complement(14693..15358)
                     /gene="csn2"
                     /locus_tag="VBC23_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6843070.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     gene            complement(15345..15686)
                     /gene="cas2"
                     /locus_tag="VBC23_001119"
     CDS             complement(15345..15686)
                     /gene="cas2"
                     /locus_tag="VBC23_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6843071.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            complement(15683..16552)
                     /gene="cas1"
                     /locus_tag="VBC23_001120"
     CDS             complement(15683..16552)
                     /gene="cas1"
                     /locus_tag="VBC23_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6843072.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            complement(16554..20666)
                     /gene="cas9"
                     /locus_tag="VBC23_001121"
     CDS             complement(16554..20666)
                     /gene="cas9"
                     /locus_tag="VBC23_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6843073.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
BASE COUNT         6588 a         4016 c         3228 g         7044 t
ORIGIN      
        1 atctccaacc tttttttatt tttaattcaa aaaatttttc aaaatatcac cgccataaat
       61 ccacaaatag ctataaccga tgatagaaat aggtttcgtt atcataatga ttgtcacaaa
      121 gcgcttaata gacatattag atagaccggt aatcatcacc atgatgtccg ctggtgaaat
      181 gggagatgcc atacaaaaga taaaaaactt ttcatatccg gatgtttcta gcttagactc
      241 atatttatca aatgtcttct gatccataaa tagcaaaacg aattttcgtc cataaaattt
      301 gactaaccaa aaaaggatga cacttccgat gataatccca atatagttat aaataaagcc
      361 taaggtagga ccaaaaatta agaaacctgc tactgttgta acacctccag gaattactgg
      421 aaaaacaatc tgtacaattt gagccacaat aaaaatcatt ggaccccaaa attcatactt
      481 gtgtactagg tcttttaatt cattgctatc attcaaaata ccaagtttgt acaaataaac
      541 aactaagaca agggtcgcta ttaacgctaa gacacccaaa gccttaatga ccttctgcca
      601 aaagcggtaa cgttttgata atttcatatt catgctttaa tcataacaca actgttaaat
      661 ttttgctatt ttaaatagta tttgttatac tatattggta agtttttatg ctttattcta
      721 cttttggggt cgttacggat tcgacaggca ttatgaggta tattttgcga ctcatcggca
      781 gatgtaaaat gccagttaaa tataactgca aaaaatacaa attcttacgc attagctgcc
      841 taaaaaacag cctgcgtgat cttcacaaga ttgtttgcgt tttgctagaa ggtcttattt
      901 atcagcaaac tacgtttggc tactgtctag ttagttaaaa agagatttat agactcgcta
      961 tgtgagggct tgagttatgt gtcatcacct agttaaatca atacataacc tatagttgta
     1021 gacaaatata ttagcagatg tttggacgtg ggttcgactc ccaccggctc catatcattt
     1081 tatcataact tactatacat acttaaaacc ttgttattac aaggttttta ttttgtttct
     1141 ttgctcatac attgccccta tcttaggaaa aaaatacttg gaacagactc taacattgaa
     1201 ttaggccacc tcattagctc actctttgtc aatatccgag aataagttct ttatcattct
     1261 aatttataga atttattaaa aaagataagg tttttagagg aaaaaatgtt ataatctatt
     1321 tagcctacga ttggaggcta gttatggcta gtattttaaa aaaacattta gagaaacgtt
     1381 tagagcttaa aaatgatcct atgaggacag atatgagtac tggtgccagt taccctaaat
     1441 atggattctc ggtaggaaag tacacatatg gatatcaaca attcttttac gaaggcgtta
     1501 acttaaaaga aattggagca ttttgctcaa tagcccagaa cgttactatt actggtatga
     1561 atcatccaac cgatcacatt accactaacc cttttattta ctataagtca aggggattta
     1621 taaatgagga tagggcggac ttaatagatg aaaagaaaaa tggaaaagtt attattggca
     1681 acgatgtctg gataggaact aatgtcacta tacttccatc tgttacaata ggtaatggag
     1741 caattattga agcaggttca gttattacca aagacattcc tgactatgct gttgtcgctg
     1801 gaacacctgc taaaattatc aaatatcgct tttcagagga agaaataaca ttattaaatg
     1861 catctcagtg gtggaattgg tcagatgaag ctattaagga acatattagc gaattttcag
     1921 ataagaggga attcttcaat actctaaaaa gtatttctga aaacaaaaat cataaactct
     1981 agaatgactt catcaaatag cacactaaaa tactactcag cataatatga gtagtatttt
     2041 agtatgctat tttgctagtt tatttagcac atgtttagac taatgcaatt ctaggaaagc
     2101 tacttagtct ctctgctatt atcatgataa gctccccaaa atatggttac taataatttt
     2161 aaagatttgt taaataacct ttttttatca tcatctagtt ctctaaagct atgaaaagct
     2221 ttcatcattt ttgctctgga agccaagttg aagtcatcca aactagatac gccacttgat
     2281 aggaagctat caaataataa atcaaaaaat aattttcttt cttgactttt taattcagcc
     2341 atccactgtt gaaatgtctt ttcaaaacaa atacttttta gcgatagtcc tgctccagtc
     2401 tttaaaatag ctggttcttg aatttcccaa gttgtaacat cgtgttgcag aattccacgc
     2461 gcattgctac taataattag cggctctata tcactataaa gcatcatacc aacaatagat
     2521 tcttctggta gtaagcgaat gcacttttgt ttagtagttt tatactctgt agtaggtaac
     2581 aaagtttttt gtagtcctgg ggagtcaata agccaaatca aatcaatttt tgcttttaaa
     2641 tctggctttg taaacattgc agcgtacaag gctaaattac cacccttaga atgacctgag
     2701 agaactactt tatcaaaata aggcagtatt gtatttaagt atttaatcgc tgaacgatga
     2761 gccgatattt ctctcatata cgtcaattta aaatcttctt tccaaccaat aaggttggca
     2821 tcagtaccac gaaatacaac ctgatgataa tcaatattag gaatagtaaa aaccatagct
     2881 gcaaattgtt tttcaaattc taaactaatc tcagcttgat aataagataa agttaagttt
     2941 ttaaaacgtt tcgaggtcat cattaactga cataaatcat gtcgttgtga cgtgaacata
     3001 aagctatatg atttagccat agtttgaagg tactcatgat ataactcaca gactaaaact
     3061 gacttcattt ctgtagtgtt tataagtttt tcaaaagaaa tatagccaaa ttcatttaaa
     3121 caaagaatgt caacatcgtt taatgctaat tcatcaaaag ttaagtttga attatttttt
     3181 aaataagtta ttatatttga catcaccgtt tattctttct actgctgaat aatttccgaa
     3241 cttcatctaa acctatcacg ataaacgggc agcttattaa atagagccaa tgtgataaac
     3301 ctaggcttgc tgtgttgaat aagttatgga atagtggcac gtatactaag actaaaaatg
     3361 ctaaaatctc cattattata ccaaaattaa ttaatttatt accaaaaata gatagtgttt
     3421 taatagattg atagctcgtt ctgctattca taaccatacc tatctggcta aagataatag
     3481 ctccaagtgt catagttgtc gcttctctgt acggtatgcc gtttgcgacg aaaaaagtaa
     3541 aattaccata acgtaagtaa tgagcccaga aaaaaccacc catagctaat acggattcaa
     3601 tagtgccgta ccaaaggaaa gattttataa gtagcccttt gtccaataag cgatctgtta
     3661 gccgtcttgg aggacgattc ataacatctg tttctggtgg ttccacgcct agacctaaag
     3721 cgggaagcat atcggttccc aaatcaacag caagtatttg cattacagtc aatggtaaag
     3781 gaataaaacc tttagaaaaa aggaagaatg ccgacggaac cgcctcagga gtattactat
     3841 taaaaatata cgtcaagaat ttcttaatgt tttgataaac agcccgccct tcttccacgg
     3901 catgtacaat agaagcaaaa tgatcatctg tcagaatcat atcagctgat tctttagcaa
     3961 catcagtacc ggtaacaccc atggctacac caatatcgga tttttttagc gcaggcgcat
     4021 cattgacccc gtctcctgta actgctacta cttcacccat ctcctgtaat atcgttacta
     4081 ctcgatattt ttgttctggt gctacacgag caaaaacgac ttcacctgac aattcttttt
     4141 tcaattggct atccgtcatt tctgataact ctaaacctga aattacttta gcatcatcgt
     4201 tacgaataat accaatattt ttagcgatac tcagtgccgt taatccgtaa tctccagtca
     4261 ccataataat acgaatgctt gctgcatgac atttatcaat agcttcacga acgccttcac
     4321 gaggtggatc actcattgct atcaacccta taaacaccat gtgttgttcc aaattttctt
     4381 gcgtccattg ttcttttgaa aagccttcaa tatcgcagta tgaaacagct aagacgcgaa
     4441 gtccatcttt agcaaatgta tcattagcca actgtatttt attacgttct tccttattta
     4501 aggggataac tttgccatca gataagacat aatctgataa atcaagtatt tctttcggcg
     4561 caccttttgt gattgaaatt tttttatcct tttcatcgcc tcctaagcta tgaatagttg
     4621 tcatgcgttt tctaaccgaa tcaaaaggta actctttcaa acgtggtgca aacttacgat
     4681 tttcttgtat gtttatccct gatttttcca ataaaacatt caaacaagct tctgtcggat
     4741 ctccaagtac agtataagtt gaacgatcat catttggtgg taatacttga gcgttgctac
     4801 agagatgagc aaaacgaata agtttttcta agtctcctct atcactatta ccaaagcaaa
     4861 tattgtcacc ttcaaaaaga atctgtcctt ctggagcata acccagtcct gtcacttggt
     4921 aactctttcc attttgccac aaatgattga cggtcatttc attttgcgtt aatgtaccag
     4981 tcttatcaga acagataact gaggttgctc caagagtctc aactgaagaa agttttttga
     5041 caagtgcgtg ttctttagcc attcgttgga ctgccatagc tagagaaagt gtaacggtag
     5101 gtagtaaacc ttctgggata aatgcaacga tcatccccaa ggcaaagata aatgattttg
     5161 acactggctc cttaacaaaa aaagtcgctg ctaagaaaaa gataattcct acagtaattg
     5221 aaatgataga aatctgtttg gtcaaccgat caagttctct ttgaagaggg cttttttctg
     5281 atttcatccc ttgtgttaaa tcagcgattt gaccaaattg ggtttgcatt ccaattgccg
     5341 taacaaccat tgttgcgcta ccactggaga ctgtcgttcc tgcgaaaacc atattatcac
     5401 attcaatcaa tgttttagac tggtagtttt caacattgct atctttataa ataggatttg
     5461 attcccctgt caaagctgat tggtttactt gcaaatctgt tgttttaatc aagcgaccat
     5521 ctgctgaaat acaatctcct tcttcaataa gaacaatatc tcctgggact agttgctctg
     5581 ataatacctt ttcttctgag ctttttctaa gaacacgtga ataagatggt aacattttct
     5641 tgagagcttg tgttgcttgg cttgcacggt attcctgaat gaaactaaaa ataccattaa
     5701 tcacattaac catccaaatg gcaagaccta gctctaagct attagaaaca atagccacaa
     5761 atcctccaac ccataatagt attgccataa gactagtaaa atttttcaga aacaataaaa
     5821 taattgattc ttgctttact tcagataaca ggtttaaacc atattttttc tgacgtacag
     5881 caacctcttc atctgataaa ccaccttgag ttgtccctaa ttcttcgaaa acctcttcta
     5941 attcctcttt agcaaaacgt gtgtttgtat ctgaaacatt atttgtagaa gccatattct
     6001 agtctccttc tctaaaacgt tttcattttt gatacctata ttatactctt ttttcagaaa
     6061 ataaactgat gaagaataca cctgccacca attcttttta ccatacataa aaaacgactt
     6121 aagatatacc cttaagtcgt ctcttattca tcaaaaagtg ataagtagtc cccatagcct
     6181 ctctttgcca tctggtcata aggaataaaa tctaaggcag ctgaattgat acaatagcgt
     6241 aagccacctg catcaaccgg tccatcatta aatacatgcc ctaaatgaga gtcagcttga
     6301 cgactcctaa cctcaatacg atgcattcca tgagagtgat cttgatgatt tgtcaccatt
     6361 cgattctcaa ttggttttga aaatgctggc caaccacaac cagattgaaa tttatctaaa
     6421 gaagagaata aaacctctcc actgacgata tctacatata tcccctcttc aaaaaaatca
     6481 tcatacttcc cagtaaaagc atgttccgta gctgaatttt gggttacttg ataagctgta
     6541 tgtccgatac gctgacgtaa ttcttcttgt gtttctttca ttatagtatc tcctccattt
     6601 gagtttgttg aggtttaaaa cctaattttt cataaaaaga gaccgctctc tgattatcat
     6661 tccaaacatt taaagttata ttataacacc catgtttctt agcatattgt cttgcaaaat
     6721 ccatcaactt ttgtccaatt tgctgacctc ttacttcctc atcaatgcac aaatcatcta
     6781 tatagagagt tttaaaggac tttcgaggta agtcacgttt ttcttgtaat tgaagaaaca
     6841 agtgggcaac aactttaccc tcgtctgatt cataaacaaa gataggcctt gattcgtcca
     6901 tcagcatttc tttcaattgt tctttagaaa acttacttcc tcttgtatga aaaatatctg
     6961 ggcgaacccc atgatgtaac actaaaattt gtttcaatag ctctgcaata gcaggcaaat
     7021 ctttttcttt cgctctacga atcatatcta ctcctaacct ttaccattcc attcagcata
     7081 aaattcggct aaatatagtt ctaaaaaatc atgacgtttt tgagcaagca ttttcccttg
     7141 ttttgtattc ataaggtctt ttaactttaa taatttttcg taaaaatgca tgatagctgt
     7201 atcctgacca ttacgatact cttctaaagt gaggttttct cgtggtttta agttagggtc
     7261 atgaattaaa cgtcctttac tccctgaata agccatcgtt cttgcaatac caatagcccc
     7321 catagcatct aggcgatctg catcttgaac aatttgacct tccaatgttt tcatagtcaa
     7381 tccagttcct gctttgaatg aaatattttc aagaatatca aggatatgtt ctacttctgc
     7441 tatggcaaga tcttgactat ataaccattg agtcaattca tacgacgctt gcttactatc
     7501 ttggcagatt ttgtcatcaa taatatcatg caaaagtgca gccatttgac aaataaaggt
     7561 attagcacct tctttgtgtg ctaattctac tgcaagattt cttactctaa caatatgaaa
     7621 ccagtcatgg ccacttggat cattttttaa tttatggtaa accatatttt cagttgcatt
     7681 taaaatcaca tcttctcttt taaccatagt catattatag ctaattttct aaaaaaataa
     7741 atgatttagc atataaatgc aaaatcctcc aagaaattct tgaaggattt tatatattag
     7801 ctccgtaaaa gattaatggt gatgatgacc tacatgtgca tgatctgttt cttcatggtc
     7861 atgatgttca tcatgtgttt tagggttttc ttttttacta tcagcattcg cttgagcaga
     7921 tttttcagtg tttttttggt gattagtatt atctgaaact tgtttgtcct ctttagaagc
     7981 catagctgct ttttcagcaa gactaccatt tccaaccatt gaaccttcta caactgctgg
     8041 aaaattctta gctactgctt caggaatatt tttttcattc aatttcaatt gtgatgttgg
     8101 aacattgtta tggctaaaat caagatttgt aactgttttg ttaggtgttt taatatctaa
     8161 agatttaagt ttatttctag aaacacttaa ggtctcaagt gaagtataat tattaacacc
     8221 ttcaagtgat gttaactgat tattagtcac attcaagaat tttaacgaac cttgtttatt
     8281 tttcaatact agtgacttaa tttggttccc ttcagcttct actttaacaa tttcatcgct
     8341 ctcttcaata ccatctaatg atgataattt cgcattatta attgttaaat tagatacctt
     8401 agaattttgc tttaagaaac ttagattgct tgtattcgtc tcatttaaaa gaagcgtctc
     8461 aagactagtt gtttttagag tcgataagtc tgcggattta ttgtttgata aatctagaac
     8521 tttcaaatct tttttaccag aaagtgcact taaattcttc acattattat gatcaaggtg
     8581 caattcttgg agttttgtca agtttgataa cggtgtcaag tcagagatat tgttatgact
     8641 caaaactaag aattgaaggt ttggtaattc agccaatggt tttagcgaag taatgccatt
     8701 attagctgca gcaataagac ttaattgttt atattttgta aggaaagata aatccttaat
     8761 accgttttgt gaaatatcca atccttctaa tagtggcatc ttatcaagga aagaataatc
     8821 tgtaatacca gtatttgtca tccaaagctg tttaagtttt ttgaattgca aaactggcgt
     8881 catatcatta atcggagtaa acccaattcc tagaacttca atattaggca tcaatgatag
     8941 accaaaacgc tggaatggat tggttctttg tccaatgtta atcttagtaa cagttgcaag
     9001 ccactgacgc attttttcag gattagtttc atttgatggg aatggtgttg gagcatctgc
     9061 atgcagaata tcctgaataa tctcatcatc aaaaccaatt tgttttagag ttgccatacc
     9121 aactttatct tttgcatgag agtgatcgtg gtgtccatct gggataggct tcccaatttc
     9181 gatatcctta cttgctatag tgtgtgagtg gtcatcatgt ggatacttaa agatgatact
     9241 tccatcctct gacgttaata cttcaatttg attaggattt aaaccaagct tctctgctaa
     9301 ataaattaat ttcttttgat tttcatcaga taatttattt gatttagtgt gctcgtgttc
     9361 ttcatgctta tggtcggact catcatggtg atgacctttg tgatgatctt cgtcatcatg
     9421 atgtggattt ggaattggct tacttagatc aatatctttg gccataataa catgtgtatg
     9481 atcatcatgt gggtattcaa atccaatttc accatttagt gttttaagga ctctgatacg
     9541 tgaaatatct aaatgtaatt cacgagcaag gtaagctagc ttatctttga tgtccttagg
     9601 taaagtattt tcatttcttg gctttttact tgatggtgac tgttttttag aatcagcttt
     9661 cttttgtggt tggtaacggt cagcaattga tccccacccc ccttttctta aatcagcaaa
     9721 actaataaaa tgcagatgat tatgatgttc tactaaaata cctgttgggg tagtgccttt
     9781 tattcctcgt ccattaaata agaaaccatc acttgttggg tagtgaagcc ctgcaatttt
     9841 agaactatta accctgtaat ggctattact ttgatttcct aaacgtgaaa cagctctact
     9901 tgcttgtact tgcttttgct gagataaact tgcttttgga atatagtgga aatggtcacc
     9961 atgtctgacc acgtagccat tagcatcttc tgaaacaata tctgcgggat taaaaacata
    10021 gccatcattt aatgtattaa cagctaccgc ccctgcttta aagtttctta ccgcttgatt
    10081 tttattagtt tttgcattag cattagggat taaatagcta aattttgaac cttttaaatc
    10141 agagtaaaaa ataaaatgag aatgatttcc atgtgctaca acgattccag aatctgtatg
    10201 agataaaatc ttcgaatctt tagtcagtaa aaagccatca tcagttggaa agtcaacccc
    10261 tgcaacaccc ttgcgttttt tatgactttc cttaacagag actttctttt tattagcttt
    10321 tttcttagca acctgctttg ttttaatatg ataagaattt ttgtgttctt gagtatgaca
    10381 tgccgtaaga gcaaaatggc atgacaggac aagaccagct agagcaataa ttttttgacc
    10441 tttcatgagt cctccataaa tgttttttcg ttttagttaa ctagttaatt atatcagatg
    10501 tttttctttt tgcaatacta tttgccatct ttttattatt tatttgctaa caaaaactca
    10561 tttatagctc atttatattt aaataaagat attataaagc catattattt gataattgca
    10621 gacaaagaaa aagtaactta agcacgaagc taagttactt taaaatagat aaatgatatc
    10681 atctttctga attatttttt gtcatcatcg tccattgata aaacacttaa gaaagcttcc
    10741 tgaggaactt cgacggaacc aattgctttc atacgctttt taccagcttt ttgtttttca
    10801 agcaacttac gtttacgtga aacgtcacca ccataacatt ttgctaagac atttttgcgt
    10861 agtgctttta tatcagaacg agccacaatt ttttgtccaa tagctgcttg gataggaact
    10921 tcaaactgct gacgaggaat gatttttttc aacttatcaa cgatcaattt accacgttca
    10981 taggcaaatt ctttgtgaac aataaagctt aaagcatcca ctttatcacc atttaaaaga
    11041 atatccattt taactaactg tgagcgacga tattcggaaa tttcataatc aaaactagca
    11101 tagccacgtg ttgatgattt aagcttatca aagaagtcaa acacaatttc agctaaagga
    11161 atttggtaaa taacattaac acgattatca tcaatataat ccattgttac aaagtcacca
    11221 cgtttacgtt gtgctaactc cattacagca ccgacaaatt cttgaggaac cataatttgc
    11281 gccttaacat atggttcttc aatgctgtca acacgagtag gatcaggaaa ttcagatgga
    11341 ttcgaaactt caagcatttc accatcagtt gtattaacat gataaactac tgatggtgca
    11401 gtcataataa gatcaatatt gaactctcgt tccaagcgct cttggataac gtccatatgt
    11461 aaaagtccta agaaaccaca acggaaacca aaccctaatg cttgtgatgt ttccggttca
    11521 aactgtagac tagcatcatt caattgtaat ttttcaaggg cttcacgcag atcgttatac
    11581 ttatttgatt caattggata aagaccggca aaaaccattg ggttcatttg cttatagcca
    11641 tgcaaggggt ctatagctgg gttattagca agggtaatgg tatcaccaac acgtgtatca
    11701 gcaactgttt taattgaggc agcaatatac cctacatctc cagttgctaa aaaatcacgt
    11761 ccaactgctt tgggagtgaa aataccaacc tcagtaacgt cgaaggtttt accattagac
    11821 atcatttgaa tcttatctcc tggcttaacc ataccattta caatccttac ttgcagaatt
    11881 actccacgat aagcatcgta aacagaatca aaaattagag cttgaagagg agcatctact
    11941 tccccagtcg gagcaggaac tttttcaacg atctgctcta aaatttcttc aataccaatc
    12001 cctgccttag ctgaggcaag tacagcttct gaagcatcta gaccaataac atcctctact
    12061 tctgcacgta cacgttctgg atctgctgct ggtaaatcaa ttttattaat aactggtagt
    12121 atctctaaat cattgtctag agccaaataa acatttgcca gagtttgagc ttcaattcct
    12181 tgggctgcat caacaaccag tatagcaccc tcacaagctg ccaatgaacg tgatacttca
    12241 tatgtaaagt caacatgccc tggcgtgtca atcaagtgaa aaatgtaagt ttcaccatct
    12301 ttagcagtat aattaagctc aatagcattt aactttatag taaacccacg ctcacgttct
    12361 aggtccatag agtccaacag ttgcgcctgc atttcacgac tagaaaccgt ttctgttttc
    12421 tctaaaatac ggtctgctaa ggttgatttt ccatggtcaa tatgagcgat aatcgaaaag
    12481 tttctaatct tttcttgacg tttcttcaaa tcttcaatat tcattctatt tcctatacaa
    12541 tttgatatgt ttaacttcta aaatattaaa acagtcctat agactctaga atattatatc
    12601 aaaaagaaga ccgatgtaaa agagaattaa cacttgaaca tcaatcttca ttaagtctat
    12661 tagttattga accaaatggc tatctcgcga gctgcagatt caggtgagtc tgacccatga
    12721 acaatattac atgtcgcttg gtttggagag ggagcttgtg caaaatctcc tcgaatagtt
    12781 cctggaaggg catctttggg gttagtactc cccatcatgg tgcgccaagt tgaaatgacc
    12841 tcttctcccg aaataactcc aatgattact ggaccactag tcatataatc tactaatgtt
    12901 ggaaagaatg gtctatccgt cagttctgca taatgtcttt tcaaaatatc tgcatctgca
    12961 tgtctaacct ctaagcgatc aatagagaag cctcgacgct caatacgact aataacttct
    13021 ccaataaaac cacgttttac accatcaggt tttatcataa aaaaggtttg ttccataata
    13081 ttagcacctc atttctttct ccactattat aacatgaaaa tatgttttct gtaacttcca
    13141 actgtatttc aaaaaaggag tgtcggacac tccttaacta tattaatagg tgagatccat
    13201 catagctgtt agttgattat ggataggttc tccactgctt tccttgtaag cttgcaagta
    13261 ttagtgacaa agaaacacca gcaatcacta aaagaaccaa cgctgtcaaa ctaataatta
    13321 agtaaagcgt agaacaatct agccctttaa ccataaggtt ggaattatag tctttcgtat
    13381 ctcttctatc gacacattct ctttgaatcc gaaaaataat tcgaattttc tactaataga
    13441 cacacctcta aatgacgttc atatttttta gtcgccaata tataagtttc ccaatcaaac
    13501 tgataaactg gggtgatttc gttagcttgg ccaactaagt tctgaattac gtcttgaatt
    13561 aatctaatta ccgccttccc ccacattctc tgaaaattta tttcagtata acaaaaaact
    13621 agaacaccac aacggtgccc tagcataata accgcacagc tctaaaacac atatcaggaa
    13681 tataaaaatc gttcaaatgt tttggaacca ttcgaaacag cacagctcta aaactgcctt
    13741 ttaattcagc tcccactttt tatcgttttg gaaccattcg aaacagcaca gctctaaaac
    13801 ttgtatatga cccgttaccg ctatcaaata gttttggaac cattcgaaac agcacagctc
    13861 taaaacttgt atatgacccg ttaccgctat caaatagttt tggaaccatt cgaaacagca
    13921 cagctctaaa acttgtatat gacccgttac cgctatcaaa tagttttgga accattcgaa
    13981 acagcacagc tctaaaacca tgatgttatg gtatacggac aatacttagt tttggaacca
    14041 ttcgaaacag cacagctcta aaactaaata ctttagaaga aagagttggt ttttgttttg
    14101 gaaccattcg aaacagcaca gctctaaaac tcagaaaggt tgatagaata atgtttccaa
    14161 gttttggaac cattcgaaac agcacagctc taaaacagtg tattcttggt tatctttagc
    14221 tatattgttt tggaaccatt cgaaacagca cagctctaaa acaccaaaat aagtctttaa
    14281 aattgctttg gcgttttgga accattcgaa acagcacagc tctaaaacta gaaatcctta
    14341 ggcgttcctc ttaaagtagt tttggaacca ttcgaaacag cacagctcta aaacttttcc
    14401 aatcacggtt catgttccaa cggtgttttg gaaccattcg aaacagcaca gctctaaaac
    14461 ttgactgatt ttgcccaatt gctcgtgcaa gttttggaac cattcgaaac agcacagctc
    14521 taaaactttg cttctaaaaa aaatacagat gaagatgttt tggaaccatt cgaaacagca
    14581 cagctctaaa acctcgtaga aaattttctt ttgagctttc gtaatcgcgc cattcgtctc
    14641 agcaggactt cagtttcgat gattccttgt tattactgtg cttttactaa tattatacca
    14701 tattttcgcc tatcaagaaa taatccttat caataacata ttgcggtaaa tcatagagtc
    14761 ttctaggttc tagaaagagt actgactttt gcattaaatt gatgtattca cataatttta
    14821 taacttcatc tttggtaaga taagctccgc tattaacaaa aaccaagaga ttctttttcg
    14881 ttaaataatg gtaaacttgt ataatttcaa aacatttttc aaagatagtg tcgctctgtg
    14941 tctcaatttt gactcctagt gccttaatga gttctaaaat cgtgatttca tcgtattcta
    15001 aatcaagctc attctctaga cactcaaatg cgataagttc tgtaatagta gctgctaatt
    15061 tttctaccat tgatttcact tctggctttt cgttgaattg attttctaag tcaccatgta
    15121 tcaactttag aataggtgct gagttgacat cgtatcctaa gatatctgta accatcatta
    15181 actctgttgc cttcagactt ttctgcttat catcaaataa tttcaaatgt tcatctacgt
    15241 catattgata aaaatgtttc accaatgaag aataaactga aacatcttct atcgttaaaa
    15301 tcgtagcatt acttaacact aatggttcat ctaaaattgg aaaattaatc ttgatcataa
    15361 gaatctccta ggaaaaccaa gcgactatca aagttggcaa cacttgtgtt gcgttctcca
    15421 tggaggtaaa ccattctcgc aaattgtttt tccgtaactg ttaagagtgt gatattaccc
    15481 ttcttaggat tattcacttt aagccgacct atcatagcat tattagctgt attattgagt
    15541 aataatttac tataaacaga gaactgatgc ataataaaac cttcgctcaa aagaaacttt
    15601 ctaaacttac gatacgcctt acgttcttct actgtttcag taggcatatc aaacattaaa
    15661 atcattctca tataccgata actcatatcc taaactccgg aatcccattc ccatcactat
    15721 ttagcgactt aataactttt ttggtgtaat cgctgacaat atttgaaaga tacatttctt
    15781 taccgtcgta actgtatgtc tctgaaaaca tagaaaagag ttctcttttc attttgacaa
    15841 aatcactctg cctattttca taaataatcc tatcaacgat tgggcgaaat ggttccataa
    15901 tatcgcttgc taggttgaac tgattaaatt gattagcatg ctttaaccca aattgtgtca
    15961 tgcaaccaca cttaacgact tcacgcgcaa acatactcaa aagcaatgaa tatccgtagt
    16021 ctaaaccagc atttattgga ttatcatgtt ctcttgaaaa atcatttcca aaaagtgtat
    16081 tgaaataaat cctagctgca tgcccttcac gattggaagt atcaaaagat tctaaatcat
    16141 gatagagatt cataatagac tgggattttt caaagaaaga acactcacca agataaaaag
    16201 actgattcaa aattttttgt gcaatgattg acgtccaaac atccgctttg acatcttcaa
    16261 tccatgacat ctgcctagat aattgtaaac tcgaatcata tctggcatag tacggcatta
    16321 acatagctgt tggcaagcgt ttgtcatcac aaaatataac taaaatattt tcatcaacca
    16381 aacgtttaat cagcatggtc gacaaaacga tatctgttgt ttccatgatt agaatgtcaa
    16441 tctctgatag atgaatcatt tccgtctgat aagaatcttt aaaaaccaga tgattatttt
    16501 tataagagag cttagaatgt gtatttacaa caacggttcg ccaacctgcc atatcaatct
    16561 tctcctaact tacccaaatc aatacgtgtt tcataaagtc cagtaataga ttgatgaatt
    16621 agggtagaat taagtacttc tttagttgat gtatagcgtt ttctatcaac tattttatca
    16681 aaaaatttaa aagctgctgg agctcctaga ctggtaaaag taaatagatt gataatatta
    16741 ttagcaagtt catctactga tatattttcc ttattatctt ggtaaagctt attgattttc
    16801 tctaaattag catctgctag aataactcga tttgaaaaat cgttgattat ttgaagaata
    16861 tcgtcgaaat aagaaacatg ttgaactaca aattcctgtt tttgttcaat ttcttctggt
    16921 tttcctttta gctcattgta acgacttgca aggtataaga acttcatata ttgtgttggt
    16981 aaggctagct cgttaccttt ttgtaattca ccagcactag caagtaatcg acgacgccca
    17041 ttttctaatt cgaacagact atacttcggt aaaatcatta atttatcgtc cctaatattt
    17101 aaataacctt ttgattcaag gaaagctgat ggatttttct caaatctgaa tctctccatg
    17161 atggtaattc ctaaaagttc cgtaactgtt tttagttttt gtgctttacc ttttttgata
    17221 tcagctacaa ctaaaacaga gtaagctact atcggactat caaaacctcc atatttctta
    17281 ggatctaaat aaatatcctt cgtttttctt ggaatcaact tatctgagtt accatgcgcc
    17341 aagattgatt ccttagagaa accacctgtc tgaatctctg tcttcttcac gatattgacc
    17401 tgagggtatg acaagacttt tctaactgtc gcaaagtgtt tcttcttatc ccaaacaatt
    17461 tcacccgtat cattattaac ttcaatatca tcttttacaa caacggttcc atccgccaaa
    17521 gttaccttag ttttaaagaa gttcataata tttgaataga aaaatagctt ttctgtagcg
    17581 gatttacgcg ttttgtaact gttatatttt ggatagtcac cgtagacaaa ttctggctct
    17641 aactgaggat atttggttag aatagcttta gcaactactg cattaagata ggcatcatgt
    17701 gcatggtgat aatcattaac ttcacgaatt ttatagaatc caaattcttt tcggaaattt
    17761 gaaaccaaat ttgacttcaa ggttacaatt ttaactttgc gaatccttct acctttacta
    17821 tcaagctcat tattaaagcg ttcatccaag atacgggcaa catgcttggt aatttgtcgt
    17881 gtctcaacca actgacgttg gataaatctt gccttatcat cggaagttag gcctccgcgc
    17941 tctgccttag tcaaattatc atacttacgc tgactcatta acttagcatc aagtaatttt
    18001 ttccagaatg ctttgcgtgc ttttacaata tcaatgctag gaacattatc tgattttcca
    18061 cggtttttag cagatgatac caaaacacga ttatcaatag aatcatcttt tatgaaagct
    18121 tgaggaacaa tgtgatcaat atcatattga cttaaattgt caatatctag tgcttcccct
    18181 gtgtacatat ctcttccatt ttgtaagtaa taaaggaaaa gtctttcatt ttgcaacgct
    18241 tgattatccg taggatattc tttcaaaata ttgccattca agtcactagc tagattctta
    18301 acgccttcct caagaagttt atagcgttgt cgagtgttac gacgaccttg atttgttgtt
    18361 tgattctcac gcgccatctc aaccacaact tgttcaggtt cgtatcccat gactttaaca
    18421 agctcatcaa caattttcaa actttgtaga atcccctttt taatagcagg gctacctgca
    18481 agctcaccta caacttcttt tagattatct gaatgactac cagcctgtgc cttgtcaata
    18541 attggtttaa atgatagacc atcatcatgt atcaactgca taaagttgcg atttgctctt
    18601 ccatcagaaa tcagatagtc caagattgtt ttttgactct ctttatctct aatgccatta
    18661 attaacttag cagacaatcg tccccagcca gtatagtgac ggcgatagag tttttttagt
    18721 tgtgactctg taaaaagatc tttatagttt tcaagacgct tcttaatcat ttctctatct
    18781 tcaaataagg ttagagtttg gacgatatct tccaaaatag actcattatc tggattatca
    18841 agaaaatctt tgtctagtat ttttttgaga tcgtggtaag ttcccaatga ggcgttgaaa
    18901 gctttatttt ctttatctag accaataaca tctactatcc taaactcctc atattcttta
    18961 gccagaaaat ctagcaactt cttcttggat accttacgat gttccttgaa tactccatca
    19021 aagatttctt gtttaatatt gctatcaaaa aaataagtct caccttgctc atttttatat
    19081 ctaaccttag tcaactcatt atagaccgta aatttttcat aaataagact atgttttggt
    19141 aggacttttt cttcaggaag atagaggtca ttgttggtca tacgatggat aaaagcttcc
    19201 gcagatgctt ccttatcaac caattcttca aaattccaag gtcgaatact gtcatctgtt
    19261 ttgcgagtca tccatgcaaa atcactcttc tcacgtgcta gtggcccgac ataataagga
    19321 attctaaagg taaggatttt ttcaatccta tctagattct ctttcaagaa tggatagtat
    19381 tctgattgac gtcgaataat agccttcaat tctgtcaaat ggacttggtg tggaattgag
    19441 ccattatcaa aggtcctttg ttttctcaaa aaatcttcat tcttgatttt ttcaagaaaa
    19501 tactcgctac cttcttgctt ggtcaacaat tttgacaagt atttataaaa tgctccttga
    19561 ttagttttgc cttcaatata accagcgtag ccatcttttg atgaatcagt aaatatttct
    19621 tgatatttct caggcaatga agcttttacg aattgtttta actgtttcaa gtcctctcta
    19681 tgttcatcgt aacgctgaat catagaggca gaaagtggcg ccttggtact gagatcagtt
    19741 actgtaagaa tgccagataa aagaacacta tcatatagct ttttcgctac tgagaataag
    19801 tcagcaaatt catcaccaat ctgcccaaga agattttcta aatcttcatc gtagctatcc
    19861 ttagcaaatt gaagcggcgt tttatcttcc aaattgaaat gtttcttgaa gtcagcttga
    19921 tttccgacaa tcaatttcaa aaattctgca aaaatgccag tagatttttg gttagggtac
    19981 tgcgctaaaa tgcgatcttt cttcgcagac ttgctaatct tatctgttag aattccctct
    20041 acatctatgt tttgagataa caaatggtta ttttcgaagg tagtatcaaa gatttctaaa
    20101 aaagcttgat attgtctttg aatatcagta ttcctcacat caaagctatc atcctcaatt
    20161 aggaaatgtc ctctgaattt aatgatatga gctagagcca gataaacaag acgaaggtct
    20221 gctttttctt tcttgtcagc taattctttt ctcaagtgat aaatcgttgg gaacttttca
    20281 tgataatctt tctcctcctg cattgttgca aagataggat acttgctccc tctcttgtcg
    20341 tcctcaacta gaaaagaatc ctctaatcga tgaaagaaac tatcatcaac tttactcatt
    20401 tcctctgcaa aaatttcttg taaatataga atacgatttc tacgacgtgt ataacgacgg
    20461 cgagcagttc gcttcaagcg tctatctgaa gcagtatttc caccatcaaa aagcagagca
    20521 cctataagat tcttctttat gtattcttta tctgtattcc ctaagaccct catcttctta
    20581 gcaggtacct tataatcatc tgtaaccaca gaatatccga cggaattagt tccgatgtcg
    20641 agacctattg aatatggttt gttcataatt tatctccttt tatttaaaat atcactttaa
    20701 aaacgcaaac tttaaagttt tgctaatttt tacttgatta ttacattaaa ataacatata
    20761 ataattttgt tggaattatt cgaaacagca cagcacgtta aaataaggca gtgatttttt
    20821 aatccagtcc gtatttagct tgaaaaagtg agcaccgatt cggtgctttt tttatg
//
LOCUS       DATRME010000031        20255 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRME010000031 DATRME010000000
VERSION     DATRME010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20255
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            210..1139
                     /locus_tag="VBC23_001122"
     CDS             210..1139
                     /locus_tag="VBC23_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6843074.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(1196..2728)
                     /locus_tag="VBC23_001123"
     CDS             complement(1196..2728)
                     /locus_tag="VBC23_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6843075.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2837..3910)
                     /locus_tag="VBC23_001124"
     CDS             complement(2837..3910)
                     /locus_tag="VBC23_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6843076.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3903..4679)
                     /locus_tag="VBC23_001125"
     CDS             complement(3903..4679)
                     /locus_tag="VBC23_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843077.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4676..5470)
                     /locus_tag="VBC23_001126"
     CDS             complement(4676..5470)
                     /locus_tag="VBC23_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843078.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(5454..6608)
                     /locus_tag="VBC23_001127"
     CDS             complement(5454..6608)
                     /locus_tag="VBC23_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843079.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6657..7559)
                     /gene="murB"
                     /locus_tag="VBC23_001128"
     CDS             complement(6657..7559)
                     /gene="murB"
                     /locus_tag="VBC23_001128"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6843080.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(7703..8191)
                     /gene="folK"
                     /locus_tag="VBC23_001129"
     CDS             complement(7703..8191)
                     /gene="folK"
                     /locus_tag="VBC23_001129"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6843081.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(8188..8550)
                     /gene="folB"
                     /locus_tag="VBC23_001130"
     CDS             complement(8188..8550)
                     /gene="folB"
                     /locus_tag="VBC23_001130"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6843082.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(8552..9355)
                     /gene="folP"
                     /locus_tag="VBC23_001131"
     CDS             complement(8552..9355)
                     /gene="folP"
                     /locus_tag="VBC23_001131"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6843083.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(9359..9922)
                     /gene="folE"
                     /locus_tag="VBC23_001132"
     CDS             complement(9359..9922)
                     /gene="folE"
                     /locus_tag="VBC23_001132"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6843084.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9941..11203)
                     /locus_tag="VBC23_001133"
     CDS             complement(9941..11203)
                     /locus_tag="VBC23_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6843085.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(11205..12092)
                     /gene="rarD"
                     /locus_tag="VBC23_001134"
     CDS             complement(11205..12092)
                     /gene="rarD"
                     /locus_tag="VBC23_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6843086.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(12079..12945)
                     /locus_tag="VBC23_001135"
     CDS             complement(12079..12945)
                     /locus_tag="VBC23_001135"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6843087.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(12947..14230)
                     /locus_tag="VBC23_001136"
     CDS             complement(12947..14230)
                     /locus_tag="VBC23_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6843088.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            14407..15294
                     /locus_tag="VBC23_001137"
     CDS             14407..15294
                     /locus_tag="VBC23_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6843089.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNTSSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(15317..16864)
                     /locus_tag="VBC23_001138"
                     /pseudo
     CDS             complement(15317..16864)
                     /locus_tag="VBC23_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(17007..18383)
                     /locus_tag="VBC23_001139"
     CDS             complement(17007..18383)
                     /locus_tag="VBC23_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6843090.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(18457..19464)
                     /locus_tag="VBC23_001140"
     CDS             complement(18457..19464)
                     /locus_tag="VBC23_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6843091.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(19572..>20255)
                     /locus_tag="VBC23_001141"
     CDS             complement(19572..>20255)
                     /locus_tag="VBC23_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843092.1"
                     /translation="KKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQV
                     EKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTFI
                     DKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNTN
                     TGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNEK
                     SYPFMKVTK"
BASE COUNT         6871 a         4063 c         3026 g         6295 t
ORIGIN      
        1 agattaaacg accctgtaag gattttgaag ttattagaaa cactctaagc aaaaatttta
       61 aagcgttttc tctatgactt atattatatg tcatgttcct atacaaaact tacactatcc
      121 tttcaaaaaa taatactatc ctacgaaacc cattttttga tagttttctg ttataataat
      181 actatcctac gattttagaa agaaaaacga tgaactcact tgataattta ctatcacata
      241 atttagaaga taaccggcat ttattaccct atgaacacat gcatactgag gttagaaatg
      301 gttaccctga cattttattt cactggcatc cggaacttga aatttcatat gtccacgagg
      361 ggaccgcacg ttatcacatc gattatgatt tttttaatag ccaatctggt gatattatct
      421 tgatccgtcc aaatggtatg cattctattc atccgattga aaataaagag catatcacag
      481 atagcattaa atttcactta gatttaatag gatattctat tgtagaccaa gttagccttc
      541 gttatttaca accgttgcag actagctctt ttaagtttat tcaatgtatc aaacctagta
      601 tgacaggata taatgatatc aaaaattgtt tgtttgatat ttttaatatt tcaaaagaag
      661 aaaatcgaca ttttgagcta ctcctaaaag ctaaattaaa cgaactcttg tatttactct
      721 attatcatca atatgtcatt aaaaaacata ctgatgatac ctacagaaaa aacgagagaa
      781 ttcgtgatct gattgattat attaataata attaccagca aaatcttacc attgaatttt
      841 tagctgatta tatggggtat agtaagacac attttatgac cgtatttaaa cagcatactg
      901 gaacatcctg tacagaattt atcatacaag ttcgtcttaa taaagctagt gagcatctta
      961 ttaactccac tacggccatt attgatattg ccaacagcgt tggttttaat aatctatcaa
     1021 attttaatcg ccaatttaaa cgctattatc atacaactcc tcgccagtac cgcaaacaat
     1081 ttaccaacaa aaaagacggt agtacgcttg tctatagaaa tataaataca aaaaaataga
     1141 aactagttaa atccatgact agtttctatt ttttattatt taagcgttaa ttagtttaca
     1201 tcagctgctc acgtacttct ttaatttccg attcattcac aactaaaaaa atggtgtcgc
     1261 cagcattcaa gatagtattt cctgatacta cagccgatgt cttatgatga atttgagtcg
     1321 ttattagcac attttctggt aattctaaat ctcggacata ctttcctgat attttatctg
     1381 aaacagttaa ttcaatcaat gtaggtgtca ttactttatc ttttggattg aaggtcattt
     1441 tagccaacat cgcttcataa ataggttctc cctttaataa atccatgaca atataagata
     1501 ccattgttac aattccaata gccattaatt gttttaaatc gccaaccatt tctgtaacca
     1561 aaatcatagc tgttagtggt gcttttgaga ttgcaccaaa atagcccgcc attcctagaa
     1621 caacaaataa agatatctgc tgatgagata ttataccaaa gtactgcata cctactgcaa
     1681 aaaaagcacc tgctaaagat cctaaagcta gaattggtaa aaaaattcct cctggcaagc
     1741 cactactgta actcaacata ctccataaaa aacgaatgag gaaaaataat cctaacatca
     1801 tcagactaag attactccta ggcagtgata caattaagcc atttccaccc cccaataatt
     1861 gtgggaaata gtaaccaata ggaagaataa aaattaccgc taatatacca tataggtgag
     1921 atggtagatg aaatagttta cctaaataat catagatcac atgaaatctc aaaattaccc
     1981 actcatagat aaagcctaag atacccaaaa agagacccat gagtaaaaaa atccaataaa
     2041 agttgagatt taaagatggt aattctgatg gtaaagctaa aactggggtt aaaccaaaga
     2101 tattaagtga gacaaagttt gccactaaac ttgctaccaa agctgttatc caaactaagc
     2161 gagaaaaatg atggtaaatt tcttcaacta caaataaaag gcctgcaata ggagcattga
     2221 aggctgctga aagacctgct gctgcaccac tagcaattaa aacacgtttt tccattcgac
     2281 tagcattgag atactgtgca attcccttac ctgtcatagc tcctaactga atactgggac
     2341 cttcgcgacc aagcataagt cccgaagaaa tagctaaaat acccgcaatg aatttcttcc
     2401 atacaatact aaaccagtct ggcgatagca ttcctttaag ttcaccttca acatgcggga
     2461 tacctgaacc tttaatatca ggatcagatt tgataaaaaa acctattatc actgcagcga
     2521 taatactagt aaccgtaatg ctcaataaaa taattggttg ataatgggca cttttataaa
     2581 gttccataac aactaaaaaa attttttcaa tcgctaatcg aaatatgctg acaacaaatc
     2641 ctgctattaa gcctactact attcctctcc aaacaaatcc cagaattgat tcaagtgaaa
     2701 actgaaattc atttttatga ttttccatat tttcctcctt ttcttacaat tattttaaca
     2761 aaaatatcac aaactttcta ggaactcttg gtaactaaaa atacctctca tcagagaagt
     2821 acttttagtt aatagattac ttacgataca ttttaaattg taagtacaaa tcattataaa
     2881 taccaagcca tttttgaccc aagttattat aaacttctaa atgatcaatt gttttatcag
     2941 gaggataaaa tgctttatca tttttgatat ctgctggtaa cagcgctttt gctttgagat
     3001 taggggtcgc ataaccaata tattctgcat tttgtgccgc atttttaggt tccatcatga
     3061 aattaataaa ggcataagct tctttgcgat gctttactgt tttggggatt acaatattat
     3121 caaaccaaag gttcgatcct tcactaggaa caacgtaatg caaatgcttg ttcccatcaa
     3181 gcatttctcg tgcttctcca gagaatgtaa caccaatcgc tgcatctccc tgaatcatat
     3241 agcctttcat ctcatcagca acaatagcct taacgtttgg tgttagagca tctaactttt
     3301 tagaagcggc ttttaattct gatatgttct ttgtattcaa tccatatccc aaggaattta
     3361 aaccaactcc aataacctca cgtgcactat caactagcat gattttattt cgaaactctg
     3421 gacgccataa atcatcccaa tgtttaggag gagttttaac taattgatca ttataaacaa
     3481 tcccaacagt tccccaaaaa taaggaatcg aatatttatt tttaggatcg aaactcaaat
     3541 ttttaaacct agctccaata gcatcccaat tagctatctt tgaatgatca agtttaacca
     3601 gaaggttttc ttttatcatt ttgtcaatca tataatcact aggtacagca atatcatatg
     3661 tcgtcccacc ctgcttaatt ttggtgtgca tagcctcatt agagtcaaac gtttcgtatt
     3721 gtacttcaat tccagtttct tttgtaaatt tttttaataa ggctggatca atatagtctc
     3781 cccagttata aataactaac ttatctgagt taggtgttga actactcttc ttttctaagt
     3841 aagtggttaa accaaagagg atcaggataa caagaataat accgcctaaa aaggaataaa
     3901 ctctacgcat tcttttctcc cttctctttc gaaatatagt aataaccaat taccaataaa
     3961 atactaaata aaaagacaat agttgacaag gcattaattt ccaatgatat accgcgacgt
     4021 gcgcgtgaat agatttctac tgaaagcgtt gaaaagccat tacctgtaac aaagaaagta
     4081 accgcaaaat catctagcga gtaagtgaaa gccataaaga aacctgaaat aatacccgac
     4141 gacaaatagg gtaacataac ttctttaagc atttgccaag ttgaagctcc taaatcatag
     4201 gaagcattaa tcatatcatc attcatttct tttaaacgag gtaagaccat taaaacaaca
     4261 ataggaatag agaatgcaac atggctcaat aatacagaag tgaaacctaa ctgtaaacca
     4321 ataacagtaa atactaagag aaaacttgca ccaatcataa catctggtgc tactaaaagg
     4381 atattattaa gcgataaaat agaggtttga tgtctacgtc ttacctgata aatccaaata
     4441 gctccaaagg taccaataat tgtagctaat aaggcgctaa gaaaagccaa aaagaatgtt
     4501 tgcactaaaa ttagcattaa gcggctatcc tgaaacagtt ctccatagtg actaaaggtg
     4561 aatccagtaa aagaattcat atccccacct ttattgaaag aataaaaaat caaataaata
     4621 ataggaatat aaaggataat aaaaacaaat gctaaataga tatttgcgaa ttttttcatg
     4681 ctttcctctc ctttgttaac cacataatag caaccatcac taaaattaaa ataaccccta
     4741 tggttgagcc cataccttta ttttgtgtga ttaaaaaatg ttgttctatg gctgtcccta
     4801 aagtaataac tcgattacca ccaataagac gcgtaagcat aaagagactt aaactaggaa
     4861 taaagacaga ttgtacacct gctcttacac cattcagaga tagaggaaat ataaccttct
     4921 gaaaagtttg ccatgcattt gcacccaagt catcacttgc ataaatgaga ttttgatcaa
     4981 tatcatccaa ggcattaaaa atgggtaata acataaacgg taattcaatg taagctgcta
     5041 caaaaagaaa tgaaaaatct gtaaatagaa tctgttttgg gccgatacct ataaaggtta
     5101 agaaggcgtt aattccccct tgttgaccaa atatccccat aaaagcatag gcttttaaca
     5161 aaagattaat ccatgttggc aaaataatta gcattagcca taattgcttg tgttttaatt
     5221 ttgtaagcaa ataagcagct ggatagctaa tcaaaagcgt tatgaaacta acgatgcctg
     5281 catataggac cgagttaaag ctcattttaa gataggttcc tgagctaaaa aacgtctggt
     5341 aattagctaa agtaaagtgc ccattaatat caaagaacga gttccaagca atcaaagcaa
     5401 caggtgctaa tacaaataag aataaccaag ccatataagg aattgaaaat agactagagg
     5461 ttttcttcat ttctctcctc ctcgatagca ttgattaaac catcttcatg atcgtcagtg
     5521 tctacatatt cttcaatgcg cgcatcaaat tcttcttctg tttcattaag acgcataata
     5581 tgtatatctt ctggagtgaa atctaaccca ataacttcac cctcaatagc tttacgagtt
     5641 gaatgaatca tccattcatt acctaaatca tcataagcaa taatttcata gtggacacct
     5701 cgaaaaagtt gtgtgtccac tttaacttga agttttcctt catcgggtag ggtaatttga
     5761 agatcttctg gacgaataac tacttggact gattcattag ggcgcatacc gccatcaaca
     5821 gcctcaaagc gcttaccgtt aaattcaacc aaataatcct caatcatctt gccagataag
     5881 atatttgatt cacctataaa agttgctaca aaatgattga taggttcatc gtagatatct
     5941 actggtgttc ctgattgaac aatttcacct tcattcatga cgaaaatcca atcactcatt
     6001 gccaacgctt cttcttgatc atgtgtgaca aaaacaaagg taattcctaa acgttgttgc
     6061 agttcgcgca attcatattg catctctgta cgcaatttca aatccaatgc tgataagggt
     6121 tcatctagta agactacttt aggttgattg atgatagcac gcgctatcgc aacacgctgg
     6181 cgttgtcctc ctgataattt ttgaatagct cgcttttcaa acccttctaa acgaaccatt
     6241 tttagagttt cttgaacacg tttttgaatt tctttcttat ccattttttt caactttaat
     6301 ggaaaagcaa cgttttcaaa aaccgtcata tgtggaaaca aagcatagtt ttggaaaacg
     6361 gtatgaacat cccgtttatt agtgggaaca tcattgatgc gtttcccatc taagtaaata
     6421 tcgcctgtac tcgcctctag caaacctgca ataatattta agatagttga tttaccactt
     6481 cctgaggccc ctaataaggt gtaaaattta ccttcttcaa gttcaaaatt aatatctttt
     6541 aagacaaccg tgttgctgtc ttcgaatacc tttgatacat ttttaaatgc aatgattggg
     6601 ttagtcaatt ctcataaatc ctccattttt ctgactaaag taagtactaa actactttat
     6661 aatagcgact caccgataat acgaacttct ggttccaaat ggacacctga agttgattca
     6721 acggtgttaa taacatgttc aataagattt tcataatctt gagctgaacc ttctgcaatg
     6781 ttaaccataa aaccagcatg tttttgacta acctctactc caccaatacg ttgaccttta
     6841 agatgtgctt cgctaatgag ttgccctgca aaatgtcctg gaggacgttt gaacacagaa
     6901 ccacaagaag ggtattctaa aggttgtttc agctcacgga gataggttaa acgatccatt
     6961 tcctgagtaa tcatgagatg atctcctggt tttaaggcaa acttagctga tataacaata
     7021 tctccactct cttgaatgac agaatgacga taaccaaatt gcatattcct agcttcaatc
     7081 gttttgagtt caccctgtgg tgtcaaaact tgtgcagaca ataaaatatg agcaatttca
     7141 ccaccgtaag cgccagcatt cataaagact gctccaccta cacttccagg aattccgcaa
     7201 gcaaactcaa agccagttaa actatgataa cgtgcaatac gagttgtttc aatgagatta
     7261 gctcctgctt ctgcttcaat aacataaccg ttgactgtaa cagttgacag tttatcaaac
     7321 atgataacaa aaccacggat accaccatct ctaacgataa tattactcgc atttcctaaa
     7381 accatccaag gaatattttg actgttagca aacttaacaa tacgcgatag ttcaaggcga
     7441 ttccttggga atgcaagata atcagcaggt cctcctactt tagtataagt atatttcttt
     7501 aaaggctcat caaagcgaat atcgagacct tctaattctt tttgtattgt tttaatcata
     7561 acctttatct caattctgtt ttattgtgtt ttatagacac aaaaaatcgg cataaaaccg
     7621 atttctatta taccaaaaat ataggttgtt gacgatacat tatcacaatt tctgtgtatt
     7681 ttaattttaa aatcctaact gactattcga ctggttttaa ataatgaact tctcctaaat
     7741 tcaatttttc gagatagtct tctagcttag aaccattagg taaacttagt tgaggtgcta
     7801 tttcgagtaa cggaactaga acaaatgctc gcttcgtcat atacggatgg ggtactacta
     7861 aatcttcttg gttaatcact tgattaccaa aaagtaagat atcaatatct attgtccttg
     7921 gaccccagtg ctcatgtctc acgcgcttta agcgtttttc aatttcttga caataatcca
     7981 ataattcaaa aggtgctaag tctgtctcta cctgacaaca tatattaaaa aaatcttctt
     8041 ggttagtatt cccccaagca gctgtttcat aaatagcgga aatttgcgcg acttttgtct
     8101 tttgcaaatg atcaattgaa aagagagcct gcttcagaaa agtttctcta tcaccaatat
     8161 tactacctaa actcaagtaa acggttgtca tactcgcttg cgctccaatt caattccaac
     8221 tgattcatag tgcccattaa ttggtggatt gtcttttgta atctttaatg taatagcttg
     8281 tactggatga aactgtaaga aaatatcttc aacaataaca cctgctaatt tctctattaa
     8341 ttgatactgt tcgttttcaa catgattttt aatacagtca aaaacttcac cataatgaac
     8401 cgtatcaatt aaatcatcag tttgtgatgc ttttgctaaa tcaagtgata gaacagcatc
     8461 aacttgaaaa acttgcccca atgtttgttc ttccgaaaaa gcaccatgat aaccataaaa
     8521 ccgacattta tttaaataaa tcttatccat attacaccaa ctgactaatt actgtcacaa
     8581 tgtccttatt tacttcaaca ttgtgaacac gtacaatctg acaccccttt gctattgccc
     8641 atgccgataa agcagcagtc cccatatcac gttcagttac ttccctatta ccacctagaa
     8701 gataattaac agtacgtttt cgagagatcc caaataacac aggataacct aaatcacaca
     8761 cttgttccaa accttttaat agctctaaat tttgctcctc tgttttagca aaaccaaagc
     8821 caggatctat ccaaatatta tcctttgaaa cacctgcttc taaagcacgc tctgctcttt
     8881 caagtaagaa ttcacaaact tcttttttga tatcttgata aacagcttct tcctggttat
     8941 gcatcaaaat aattggaaca tttcgttctg ctgctaaaga tagcatttta ccatcatata
     9001 aacctgacca aacgtcattt aaaatatgta cacctgcatc aagggctgct tgagctacct
     9061 ctgttttata agtatctatt gataacaaaa gatcagggta gacctctcta atagctttaa
     9121 tcattggaac tacacgttta atttcttctt cagcagtcac aaatactgca cctggccgcg
     9181 ttgattctcc cccaatatct acaattgcca ccccttgttc aagcatctca ccaacttgat
     9241 ttaaagctga atcaattgtg gtataagaac caccatctga gaaggaatca ggcgttacat
     9301 ttaaaatccc catgatacaa gctttacctg taatatcata ttgtcctatt ttcatctttt
     9361 atcctttttg tattagcgat agaatttctt gtctttcata tcgatcgtcc ttgtataaac
     9421 cacgagctac cgttgtaatc gtcttacttc ctggtttttt aatgccacgc attgtcatgc
     9481 acatatgctc tgcttctatc ataacaaaaa tacctttagg cgctaaagca tcctctaaag
     9541 cttgtgcaac ttgtgctgtt aaacgttcct gaagttgagg acgcttactc gccacttcaa
     9601 ctgcacgtgc aagcttacta agtccagtaa cacgtccatc gtttggtaaa taagcaatat
     9661 gagctttacc ataaaaagga accaaatgat gttcacacat ggaatagaat ggaatatcct
     9721 tgacaatcac aacctcttca tgattttcag aaaagactgc agtgaattgc tcctttggat
     9781 cttgattaag tcctgagaac atttctatat acatttttgc cacacgcttc ggtgtatctt
     9841 tcaacccttc tcgatcagga ttttctccta aagcctctaa aaattgataa atagcttttt
     9901 ccattttttc ttgattgggc attaatttat cctatccttt ttaagttgtt gtagttttga
     9961 ttaatagtag ttcctgacga acttgagaaa taaaataaag ggagccagta atcacataga
    10021 aatctttatc tgatgtcaga tttatatcat gtagccattt cttccaatca ctaactctag
    10081 tgtacgcttt aggatatttg tctaagttta ttgatttagg gtaatcaaag ctagtaaccg
    10141 aaaccggagc gatactagac aatgattcta acatactctc tatcggttta gtattaattg
    10201 cagcaaatag aatatgcacg tttttctgct gatatttttt tataacttga acgagagcat
    10261 cgacactttc attattatga gcgccatcaa tcattacatt tggaaagatt aattctgtac
    10321 ggcctaccca ttttgtcatt tcaagcccat ctttaatggt ctgtagggtt aactttggat
    10381 atttgtctct taataataaa ctggtagtta tagctatgct agcattggaa acttggtgat
    10441 gtcccggcat ttgcaactga atatgatcaa tatttgcttg aggacctata tagttaaaat
    10501 aattttcctc ttcttttata taaaagtcat tgtgtaaacg ataagtctgg ctgtgggttt
    10561 ccttggcttt tatttgaaag acttcttcaa catcctgacg atcattagca tatacgaatg
    10621 gaactccttt ttttaaaaca cctacttttt gttcagctat atctacataa gttcgtccta
    10681 aaacctcctg atgatctaat ccaattgacg gacaagtaac cgctaaagcc ttgaacatgt
    10741 tggtagaatc ataataaccg cccattccag cctcaataat gacgatatca actggacagc
    10801 tattgccaaa ataatagaac atcaaaactg ttataacttc aaattctgta gctggttcta
    10861 ggttggtctc aaggtgcaac cgttcaacaa ctggacgaac agtctcaaca agtttgataa
    10921 aatcagactc tggaatcatt tgaccatcaa tgctaatacg ctctctaaag tcaacaatat
    10981 atggtgatgt aaatgttcca acttgataac cagaatttgt aaaaatatgt tgtaaataac
    11041 tagttgttga tcctttacca ttcgttccaa caacatgaat ggcagatagg ttgttttgag
    11101 ggtttcctaa ctgctctagc atccatctca tacgttccaa acctggttta ataccaaaag
    11161 ctaatttgct atgaatccat tctagcgctt cttgataagt catcttattt acctttcttt
    11221 aacatcgtat gtacttgtcc aatactaaaa actaaaatag caagccagat aaaaataaaa
    11281 cctattacct ccccactaac gatagtttct ttaaaaataa acaatgctag caataattgg
    11341 atggttggat tgatgtattg gataaaaccg ataatattta gaggagccct cttaatggct
    11401 tctgcaaata ataaaagagg aactgctgta atgattcctg aaagtgagag taaaactaat
    11461 tgtaatatat tataatcagt taaaaaatct ttggcgaaaa acactatata aatgagtgca
    11521 aacggagcaa taaaactact ttctaccagc atcgaaaaat cgctagataa agaaattgat
    11581 ttttttaata acccataaaa accaaaagaa agcgctagag ttagtgaaat taaaggaaaa
    11641 tgaccagttt gataaactaa aattccaact cccatgattg caatcaaaat tgcaaggcta
    11701 actactcttg ccaggtgttc acgtaaaaca aggaccgaca acaaaattga aataatcggc
    11761 atcatataat aaccaagact tgcctcagta gcgtgaccat gcgtgactgc aaaaatatag
    11821 accaaccaat taattgaaat taaaatactg gctacaaaca ttgtccaaaa tgacttttta
    11881 ttattaacta agccttttaa gtcttttaaa tagactgttt ttctacccga aacgagcata
    11941 taaataagca tagttaaaac agtgaatata atcctataag caaacgtact atatgcttca
    12001 attccagata ataatttcca ataaagagat aaaagccccc ataaaacata ggctgacaag
    12061 ccaaggataa ttcctaaatt atcttttctc aactctcact ccttttcgct caatattaag
    12121 tacttgaatc tgtccatcta aacataattt acttaactct gtgacaattg gttgttcttt
    12181 ttctttcgaa cacagaacca ttaccgtcgg tcctgctcca gacaaataag tagcatacgc
    12241 accgttttga gtgctgattt gttttataac tccaaattcc ttcaccaaag gttgacgata
    12301 tctttcatga aagaggtccc tttctatagc ccatccagca gttaccaaat cacctttcaa
    12361 caacgacgca accatcacat tagcgacgct gcttgaagca actgcatcct cataactcaa
    12421 tttttgaggt agtacatttc gactatcact tgtttttaat tcataatttg gaataaaagc
    12481 gataaaggaa ctatctggaa acatcacttc caaactttta atatcatttt tcactattga
    12541 tgaaataact aaatcaccaa aaatggccgg tgctacatta tcaggatgtc cttctaatcg
    12601 ggtagctatc tctaattttt ctttttgaga aagagccaaa tttcctaatt gattggctaa
    12661 ttctattcct gcaacaatta cagaggaaga agaacctaaa cctctggcca aaggaatatc
    12721 tgaaaacatt tttaagcggt gtggtgccaa atccgactta acatgcaaag ctgtttgaat
    12781 aaggaggtta gtatgatctt ttggaatatt tactaaattg tgttcaacta accactctgt
    12841 actttcttct aatacttcaa tgcttaaata ctttgataac gcaactccta ttgaatcaaa
    12901 gcctggacca atattcgcac tagttgcagg tacaattatc ctcatttcac cctcctatta
    12961 ctttgaaaac attgattaac tgaatgacac cactctcagg aaactcctca ataaattgag
    13021 ttattttctc ttggctaact tcatctgtta atacatagat ttcttgttgt ttatcatcca
    13081 atgctttttc aataacattt ttcaaaccta tattttcttg ctcaaagtac cttccaagtg
    13141 tatcactatc ttcagcattt ctcaaagaaa tagctagata gtactcattg aagatatcag
    13201 actgcattga caacctacac ggacaatgat aagtattcat aggcttaatt tttatctgtg
    13261 atcgaatact acgactaata tcgatgatat cggctaaaac agaggttgct gttggatttt
    13321 gtcccgcacc tggtccataa aaaagagact gaccaatccc tattgattca atgaatactg
    13381 cattcatcac atgattaact gcagctaagg gatgctcttg agaaattagt gtcggagaaa
    13441 cgtctacact tataccagtt ggagtttcac gaagatcagt taccaactta atggcaaagc
    13501 ccatctcctg agctacttca acgtcctctg agcgaatact agaaatcccc ttataattaa
    13561 catcatcaaa atcaataggc ataccaaatc caaattgact taagatagtg gctttgtagg
    13621 cagtatcaat accttcaaca tcatttgtag gatcactttc ggcataacct aactcttgtg
    13681 ccttttttag agcttgttca taagaccatc cctcttcaaa catttttgtt aacatgaagt
    13741 tggaggtacc gttgagaatt cctaataaac gtgtcatttt atctgaggca aacgaatgcg
    13801 atagggtcct taaaatagga atgcctccag caactgctcc ctcatagaat aaagctagac
    13861 ctctagcgtc tgctaaatcc atcaactctt ttccgtgtaa agcaatgaga tctttattgg
    13921 ctgtcacaat atgtttacca aatcctaatg cttgactcaa ataagtttta gctggctcaa
    13981 tcccccccat taattccact acaatatcaa tttgtggatc ttgaagaata gtatttatct
    14041 ctgtcacaaa gttataagta aatccctgat tgatgaaacg ctggcggctt tcattatctc
    14101 ttactaatac tttatcaatt acaatatctt cgccttctaa agaaagtagc ttatgttgat
    14161 tttcttttag caaatatgga atacccttag caaccgttcc aaaacctaat aaagctattt
    14221 taatagtcat gaatttctcc ttagaaattg tctctaactt caagacaaac attggctata
    14281 ttataacaaa ttaccaatat tttttatagg gtaaacgcgt cttgtcctat gaaagttctt
    14341 aaaaagagaa tattatttca gaaaagatga tacaataaaa gtactttaaa agttaaggag
    14401 aaataaatgg cacacacacc tactagtcat cgtaaaccac gaaaacgttc cccatggtta
    14461 gctattgctt ctgtcttttt tttgttaata gctttaatcg gcatatttct attcttcaac
    14521 aatcgttcaa agcaagaaat aaaaacaaaa acgaacacct ctagtcatcg aaaaattgtc
    14581 acatctatca aaaaaaagaa atgggttaag caaaagactc ctgttaaaat acccatttta
    14641 atgtaccatg ctgttcatgt tatggaccct tctgaagccg ccagtgctaa cttaattgtg
    14701 gcacctgaca tttttgaaag tcatataaaa cgtctcaaaa aggaaggata ttacttctta
    14761 gcccctaacg aggcatatcg tgcactaaac gaaaatgctt tacctgaaaa aaaagtcatt
    14821 tggattactt ttgatgatgg aaatgccgat ttctacacta aggcataccc tatactcaaa
    14881 aagtacaagg ttaaagccac taataatatc atcactggct ttgttcaaga aggacgagaa
    14941 tctaacctca acgttcaaca aatgctagaa atgaagcaaa acggcatgtc attccaagga
    15001 catacggtta ctcaccccaa tctttctctc ttaactccag aactacaaac ccaagaaatg
    15061 acactatcaa aactgttttt agatcaaaaa ctctctcaag atactcttgc gattgcttat
    15121 ccatcaggac gctacaatcc tacaactttg gatattgcta gtcaatatta taaactaggg
    15181 cttacaacaa acgaaggagt tgcaacaaaa gataacggtt tgctttctct taatcgagtt
    15241 cgtatcttac caactactag cgatgatgat ttgatcaaaa cgattaacca ataaaaagaa
    15301 gccttctggc ttctttttat tcttcataag atttaaatgg ttcattttta cgtggataac
    15361 gatctggaga aatcgataag cccatttctt ttttctcatg gaaaagctcc actaatagcg
    15421 ctagcatcat caaaatcatt acgaatgaaa atggcagagc gacaataatt aaaacgttct
    15481 gcagcgctaa cagaccacca gacaagagta agacaatagc aatcactgcc aacactagcc
    15541 cccaaattac tttagttcga ttttttggat ttaaattacc atcttctgtc aacatagcca
    15601 aaacgtacgt tgcagaatct gctgatgtaa taaaaaatga aaaaattaaa atgatagcaa
    15661 tagtggataa aagccaacct aaagtgtagt gattaaaagt agcaaacaat acttcctctg
    15721 ttgcaaactt tgtcaaatta atacccagtt gttgtacctg agttgataat gtgccaaatg
    15781 ctgcaaacca tacaaaactc aataatgtcg gtattaaaag aactaccgtt aaaaactccc
    15841 gaatgctacg ccctttagaa atacgagcga tgaaaactcc aacaaaagga gaccaggaaa
    15901 tccaccacgc ccaataaaaa atcgtccaat tatcaatcca agaacgttta gtattgtcaa
    15961 aagcagctgc acgaaagctc attccaaaga aattttgtaa ataagagcct aaagactctg
    16021 ttagggtatc aaaaatacga accgttggtc ccaatacaat aactaaagct aagagggcta
    16081 ccgctaaaat caaatttaag ttcgataaaa ttttaacacc ttttcctaaa cctgataaag
    16141 ctgacataac aaataaagct gttgtaataa ggataataac aatttgaaca aatgcattat
    16201 tgggaacacc caataaaaaa cttaaccctc cgttgatttg agcagctcca aatccaagtg
    16261 tcgtagcaac tccaataact gtagcaacaa ctgtggtgat atcaacaatt tttcctagtc
    16321 aaccatctgt cttatcaccg aaaagaggct ttaaagtgac agacaagagg tatttctctt
    16381 gctttcgaaa tccaaaataa gctagagcca aagcaaccaa tgcatatact gcccaagcat
    16441 gtatccccca gtgaaaaaat gtaaaacgaa atgcatcagc taatgctgtt tgcgattcct
    16501 taggtgcgcc aggtgtcgaa atagcaaaat gcgataaggg gtcagctgct ccgtagaaga
    16561 ccaaaccaat ccccatacct gctgaaaaca tcattgctat ccatgataca gttgaatatt
    16621 caggacgtga atgaggtcgc cctaaacgta tttgtccaag aggactcata ataaaaaaga
    16681 cacacaaagc aagaattgaa gtaactaaca acaagtacaa ccaaccgaaa ttggtagata
    16741 cctgttcacg caagatttgt gtccaagatt gaaaaccacg aggaacaata attcctaata
    16801 agactaaaat aagcgataca attagtgaac ctgtaaacac aggcgtaata tgttttttac
    16861 tcaaaatttc ctcctatact aaaatagtat agcatacttt aataaatact cctctcattt
    16921 taatcacctg tatcaagacc tctccttgtc tcctcaacaa aaaagaacca gataacatta
    16981 tctggttctt gtgtttgact tagtgattaa tcactatttg gtgtaaatac caaatgttcg
    17041 ttgacaaagg tatcaaatcc aagttggctc aattcacgac catatcctga atttttaata
    17101 cctccaaacg gtaattccgg taatgatgtc caccctgaat taataaatgt catccctgtt
    17161 tcaatctgag ccgccacttt tttagcatgt tctggatcag aagagaaaac agtacttcct
    17221 aaaccataac tagaatcatt agctaaagca atagcttctt cttcagtatc cactttatag
    17281 atagaggcta cggggccgaa aatttcttga ttatagattg gatttgcttt agtgatattc
    17341 gttaaaacag ttggcatgac gaagtttcct ggatgatcta tagtgtcatt tccaaaaact
    17401 acctcagcac cgtggtctac cgccaattta atttgtttta aaacatcatc ctttgcacct
    17461 gcagatgaca gtggagccaa ggttgtttcc gaatccattg ggtcacccca tttagcagac
    17521 ttaaaggtct caactaccat atttacaaat tcgtcatact tatcagccat aacaatgaaa
    17581 cgttttgaag atgtacatac ttgacctgca ttatataaac gcgcaaagaa tattgtttta
    17641 ctaagtaaat caaaatcagc atcatctaaa ataagaaaag catcgttccc acctaattcc
    17701 atagacgatt ttttcaaatt tttaccagcc tcagctgcta ttgatgcccc accacgctca
    17761 gaaccagtta aacacacacc tgcaacacga ggatctgata taaggtttga aacttgatca
    17821 tacgaagcaa aaatattttt aaacgctcct tctggagctc ctgcttctcg gactaaatcc
    17881 tcaaatgctt gagctgatgc aggacaaata gaagcatgtt ttaaaagcat agtattaccc
    17941 actataaagt taggggcaaa tacacgcata atctgataaa atgggaaatt ccatggctcg
    18001 acagcaagta aaacaccgac agcttgtttc aaatagtaag cctctccatt tggactttct
    18061 aacggaacag gttttaaaaa cttttgccca ttatcagcat aatagtcagc tatgtctgca
    18121 cacaagtcca cttctccttg tgcttcagtg aaaagttttc ccatatcttt agtcataact
    18181 tcagcgtact tatcacggtc cttacgtaat aaatctgcta ctttatgcaa ttgattttgg
    18241 cgttcttcaa cattatcttc tttgcgccat gtcttgtaga gggcatgcgc tatgtctaat
    18301 gactgttcga gatcactgtc actgatatta tcgaattcat gcaatacttc gttagtgtac
    18361 ggataaattg ttttatatgc cataatgttc cctccttttg atatcctcat tataacattt
    18421 atgaaatcga ttacaaatat ttagctttag acaagatcac caagttccaa ggatagcttg
    18481 cattgttaaa ctaactataa taatgcctaa ccaacacgct gcacctaaaa gaattgattt
    18541 tcctccagtt ttaattagtt tgctgacatt agtatttata ccaattgccc ccattgccat
    18601 aacaataaaa aatttagata aaaccttaag tggctcaaag atacgtaacg aaaaacctaa
    18661 tgaagcaact atcgttgtta gcaaagaagc taagataaaa taaagcacaa acttcggaaa
    18721 tatcttagta acacttacag attgttcttt ctgcgtttgt ttcatatgat aaatagataa
    18781 tacaatcgta attggaatga tggctaatgt tcgtgtcaat ttaacaatag tagctccgcc
    18841 caaagtattg ctgtgattga tagcatccca tgcagtagca gttgctgtaa ctgacgaggt
    18901 atcattgaca gcagtccctg caaataatgc aaaaccgtga tcagagagac caataaaatt
    18961 ccccagcgtt ggaaaaatta aagccgctaa gatattgaat aaaaaaataa ccgaaatagc
    19021 ttgtgctact tcatcatctt tagcattaat aactggtgca gtcgctgcaa ttgcggatcc
    19081 accacaaata gatgaaccaa cacctattaa agtagcaatc gttttatcta acttaaataa
    19141 cttttgcaaa acataagcaa taatcaatga aatggaaatc gtcataataa tgatgggtaa
    19201 cgatgatata ccaactttca tcacttgcat gaggtttagt ccaaaaccca ataagacaac
    19261 tgctgtttgt aaaatatatt tagacgtaaa agcaataccc ttattaaaca attgtcgatt
    19321 ttgataaaag gatccaacaa ttattccaat caagatagca aagactggtg ctcctattaa
    19381 agggagataa agcccaagca accaagatgg tatagcaata ataaagcata atattagtcc
    19441 aggaattttt tccttaaata acatgatagt cctcctcaca ttgactatta tatcacaaac
    19501 tttaaaacgt ggcaggaaaa ccgaaagagg accagaatat gctctgatcc tctttcatta
    19561 ctaattcaca attatttagt tactttcata aatggatatg atttttcgtt acctaaaatc
    19621 aattttgagc cctctttttt ataggctgtt ttttcaccat ttttcacaat attaccatag
    19681 aacgcatttg cctttttatt gatatgcgat actttaataa cacttgttaa gcgatgtact
    19741 ttaccatcca taactggcaa tgtcatttca ccagtattag tattatattt tcctccgaca
    19801 gcttgtgcag cactttggaa acttctatca atttgttggt caatttgttt gtcagtgatt
    19861 tttgtttcat caaaaatctt tttacctgct tcacttaaat cattgtaagt caaaccttga
    19921 tcttttaatt gtttttcaag agctttatca ataaaagttt ttatagcttg atggaatttt
    19981 acctcatcaa tttgagcagt gactgttatc tttgcctcat cgtttttgac gttcatatgc
    20041 atattagcgc ccttaaccaa atcagctgca ctaatcccca tatccgctgc ctgacttcca
    20101 agttgttgtt ctatttcttt ctcaacttgc ttctctaaag aaactgtacg ccatttcccc
    20161 tctagtgact gacgattatt ccagaaatag agaccgcctg cacctacaat tagaataagt
    20221 actagtacag ttaaaaaaac ttttaaaaac ttttt
//
LOCUS       DATRME010000032        19839 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRME010000032 DATRME010000000
VERSION     DATRME010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19839
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            132..248
                     /locus_tag="VBC23_001142"
     CDS             132..248
                     /locus_tag="VBC23_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6843093.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKHYGKK"
     gene            complement(340..1224)
                     /locus_tag="VBC23_001143"
     CDS             complement(340..1224)
                     /locus_tag="VBC23_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6843094.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(1372..3036)
                     /locus_tag="VBC23_001144"
     CDS             complement(1372..3036)
                     /locus_tag="VBC23_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6843095.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(3036..3401)
                     /locus_tag="VBC23_001145"
     CDS             complement(3036..3401)
                     /locus_tag="VBC23_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6843096.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(3533..3721)
                     /locus_tag="VBC23_001146"
     CDS             complement(3533..3721)
                     /locus_tag="VBC23_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6843097.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            3960..4877
                     /locus_tag="VBC23_001147"
     CDS             3960..4877
                     /locus_tag="VBC23_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6843098.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(4962..5843)
                     /locus_tag="VBC23_001148"
     CDS             complement(4962..5843)
                     /locus_tag="VBC23_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6843099.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(5983..7371)
                     /gene="argH"
                     /locus_tag="VBC23_001149"
     CDS             complement(5983..7371)
                     /gene="argH"
                     /locus_tag="VBC23_001149"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6843100.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            complement(7390..8580)
                     /locus_tag="VBC23_001150"
     CDS             complement(7390..8580)
                     /locus_tag="VBC23_001150"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6843101.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            complement(8734..9813)
                     /locus_tag="VBC23_001151"
     CDS             complement(8734..9813)
                     /locus_tag="VBC23_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6843102.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVSSQLEIGTEFCVELPLS"
     gene            complement(9797..10468)
                     /locus_tag="VBC23_001152"
     CDS             complement(9797..10468)
                     /locus_tag="VBC23_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6843103.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(10499..11185)
                     /locus_tag="VBC23_001153"
     CDS             complement(10499..11185)
                     /locus_tag="VBC23_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843104.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(11185..12273)
                     /locus_tag="VBC23_001154"
     CDS             complement(11185..12273)
                     /locus_tag="VBC23_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843105.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            12455..13441
                     /locus_tag="VBC23_001155"
     CDS             12455..13441
                     /locus_tag="VBC23_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6843106.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            13434..14345
                     /gene="rbsK"
                     /locus_tag="VBC23_001156"
     CDS             13434..14345
                     /gene="rbsK"
                     /locus_tag="VBC23_001156"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6843107.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            14320..14718
                     /gene="rbsD"
                     /locus_tag="VBC23_001157"
     CDS             14320..14718
                     /gene="rbsD"
                     /locus_tag="VBC23_001157"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6843108.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            14734..16212
                     /locus_tag="VBC23_001158"
     CDS             14734..16212
                     /locus_tag="VBC23_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6843109.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            16214..17155
                     /gene="rbsC"
                     /locus_tag="VBC23_001159"
     CDS             16214..17155
                     /gene="rbsC"
                     /locus_tag="VBC23_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6843110.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            17208..18176
                     /locus_tag="VBC23_001160"
     CDS             17208..18176
                     /locus_tag="VBC23_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6843111.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(18216..19670)
                     /locus_tag="VBC23_001161"
     CDS             complement(18216..19670)
                     /locus_tag="VBC23_001161"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6843112.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         6207 a         3829 c         3324 g         6479 t
ORIGIN      
        1 attacttttg attgatattt aattactttg ctatttattt ttaaatttat caacaaagta
       61 ctttaccagt taattttttt gtggtatact ggtgtctgtt aacgttaata aattgtctat
      121 aggaggtaat catggctgaa aaaacagacc tatcgtcagc ctatcgcaga cttaaaagtc
      181 ctaacattaa aactagaaaa cgtgctctga aaatcattca tgagtacaaa cattacggta
      241 aaaaataact tttttccgtc tctccaatta taataaataa gtcagacaca aaataaccag
      301 agcaaatgct ctggttattt tgcttctata aaaagttcat tatcttgttt taagagcaga
      361 taaaacttgt gtacgaatat cttcaacacc ttctgggtta tttggtaaga aaatcgtttg
      421 attaccatta atagcaaatg tatttaaagt atctaaatat tgatttgtta acaagataga
      481 catgatttgt tcttctgtca atgtcacgtt agcatctttc aattcttgaa tcgaatcagc
      541 taacccatct acaattgctt tacgttgttg cgcaataccc acaccatgta gacgatcttt
      601 ttctgcttct gcttctgcag cagttacaat tttgatctta tcagcattcg ctaattcttg
      661 agctgctaca cgtttacgtt gtgcagcatt gatttcgttc atggattgtt ttacttctgc
      721 atctggctca accttagtaa ttaaagtctt aacaatgatg tagccgtatg ttgacatttc
      781 ttctgccact tgatgttgaa cttccaaggc tatttcatct ttcttttcga atagctcatc
      841 taatgtaagt ttaggaactg atgaacgcaa agcatcttcg atataagatt taatctgagc
      901 ttctggcttt attaatttgt aataagcgtc tgtaacgtta ttttcattga cacgatattg
      961 tgtagcaata tttaaagtga caaaaacatt atcttttgtt tttgtctcaa ctataatttc
     1021 actctgtaaa agacgtaact gcactcgtgc tgcaatctta tcgataccca atggtacacg
     1081 aatatgaata ccgctagtag cagtcttttg atatttacca aagcgttcaa tgatggctac
     1141 cgtctgttgt ttaacaacgt ataaagaagt tatcaacaga acaatcacta aaaccaaaat
     1201 aaccgtaaga ataattaatg acatcataaa tctcctttgt atgaatgttt taaaacaatt
     1261 ctagcacaat ttgtcaaaat tttcaaaata ctgatgcttt atcatactaa aatagaaact
     1321 ctgataaaat aaaaaattag aagtatctct cctaattttt tattttatca attattcaac
     1381 gctcatgagg tatggatata caggttgatc tccttggtgg atctcaactt caacatcttc
     1441 ataagtctcc tcaagatatt ccgacaaggt ctcggctaat gcttgtttac catcttcacc
     1501 aacatagatt gtcacgattt cactatcttc atcaatcatt ttatcaaaag tgtctttaag
     1561 tgctttttcc atatctggtg tagatactag aattttacca tcaaccatcc caaggatgtc
     1621 gttttcatga atttcaaggc catctatcgt tgtatcacga acagctaaag tcacactacc
     1681 actaatgaca tctgagaggc tatttgtcat atcagctaca ttagtttcaa gagacttagc
     1741 aggatcaaaa gcaagtaagc tagtaaatcc ttgtggaact gtacgtgtct ctactactgc
     1801 tgctggaata tcaacgacat ctgcagctga ctgtgcagcc atgaagatgt tcttattgtt
     1861 tggtaaaata ataacatttc tagcgtttac tttttcaatc gcttttacaa tatcttctgt
     1921 agatgggttc atagtctggc cgccagagat gatataatca acaccttgtg atttaaaaat
     1981 ctcagctaga ccgtcaccag ctacaacagc aataatacca tattctttag tttctttgac
     2041 agtttcttca acttcaacct tttgcatttg ggcatcatgt tggttacgca tgttttcaac
     2101 tttaaccttc acaagcgatc catatttcaa accttcttgc ataacaagac ctggatcttc
     2161 tgtatgaaca tgaaccttga caatttcatc atcattaaca actagtaaag aatcccctag
     2221 attgctaaga taaccttgaa attcttcgta gttaaattcc ttaacgtaag ttggtccttg
     2281 ctttaaacca accatgactt ctgtacaata gccatattta atatcttctg tggctacgtg
     2341 gcctactaca gctttatggt gctctgcatt aaccatttca gtcattgttg cgggagttgc
     2401 tttaaaatct tctgatgcga tatattctcc agtcagtgct gagaggaaac cttcgtaaat
     2461 aaagacgagt ccttgaccac cagaatcaac aacaccaacc tctttaagaa caggtagcat
     2521 atccggtgtt ttagcaagag cacgcttagc acctttgaga gttgcgcgca taacttcaac
     2581 tgcatcatca gtttcttcag ctttttttag agcagcggta gctgcaccgc gtgatactgt
     2641 caagatagtt ccttcaactg gtttcataac ggctttataa gctacttcaa caccattctg
     2701 gaaagcgtgt gccaaatctt gacccgttaa ttcttcttta tccttgatac tttgaccaaa
     2761 accacggaat aattgtgatg taattacacc tgaatttcca cgtgctccca tcaatacacc
     2821 tttagaaaga atttgaccaa cttctccaac agtcgtcgct ggtttgtccg aaacttcctt
     2881 ggctccgttt tccatagtca tccccatatt cgtaccagta tcaccatctg gaactgggaa
     2941 tacatttaat gagttgacat actctgcctg tttactcaga cgtgtactgg cagcttgtac
     3001 catttcttgg aataaacttg tcgtaatctt tgacactaat cttctcctac aaccttaata
     3061 ctttgcacat aaacattcac catatcagca gataaaccaa gctgattttc gagattgaat
     3121 ttcactcgct cttgaatatt ttttgaaact tcactaattt tgacgccgta actcatgaca
     3181 gtataaacat ctactgcgat gcccgcttca ctagacctca caacaacacc ttttgcgtag
     3241 ttttccttac gcaaaagtgc ttgaaagtta tctttgatag cacttttact tgccatacca
     3301 acaacgccaa aaatttccgt tgctgaacct ccaacgacag ttgcaataac gtcatcagag
     3361 agttcaattt gaccatctct tgtattgatt tttacagtca tatctactac ctcaaaaagt
     3421 ttttatcact ctattctatc atatctcact attattgtaa aaaaatagag gtatctaact
     3481 atcaaatcag ccttagaaac actaaaaaaa gccctaaggc tttctcagtt tattaaacgc
     3541 gttctacttt accagattta agcgcacgag ctgaaaccca aacttttttt ggtttaccgt
     3601 cgataagaac agtaactttt tgaaggtttg gtttaacagt acgttttgtt tggttcatcg
     3661 cgtgtgaacg gttgttacca gaaactgttt tacgtcctgt gaaataacat actttagcca
     3721 ttttacgagt tcctcctata ttgatcaata tttacggatg tgctagcacc acatactaga
     3781 ctattctatc acaacttact tcttctagca agttcttttc aaaattactt tttcatttgt
     3841 tttatctaaa tcgcttacat attttttata ataagacaca cttccttcta attcattttt
     3901 tagtttacat tttctttcaa aatattataa tagacaacaa taatcaaagg aggtttcata
     3961 tgacacgtaa aattggaatt ataggcatgg ggcacgttgg atcaactgtt gcacacgctc
     4021 ttattgcaca aggtattggt gatgattatg tctttattga tactaatgtt gataaggtta
     4081 atgctgatat gattgatttt caagatgctc tggcaaatgc tacttatcac gctaatatag
     4141 tattaaacga ctatgacgcg ttgtcagatg ctgatgttgt gatttcagcc ttaggtgata
     4201 tttcactaca gaataacaaa gaagctaatc gctttgctga actaccattt accagtaaag
     4261 aagtctacga cgttagccaa aagttaaaat catctggttt tagagggatc cttattgtcg
     4321 ttactaatcc tgttgatgcc atcacccagc tttatcaaga atatactcaa tttcctaaat
     4381 cacgcgttat cggaacagga accttattgg atactgctcg gatgaaaaga gtgcttggtc
     4441 aacgcctaca agttgatcca aggagtatcg gtggttataa tctaggagag catggtaact
     4501 cccaatttgt cgcttggagt caggtaactg taaaaggaca accagctata tcactattaa
     4561 aagtagaaga attagaacag ataaaagaag cttctttaaa aggcgggcac actgtctttt
     4621 ttggtaaaaa atatactagt tatggtatcg ctaccgcagc tgtccgcatc gttttagctg
     4681 tgttaaatga ttcccatgag ttactacctg tatctacctt ctatgagtcc gaaggggttt
     4741 accttggata tccagctatt attggtagag taggaattgt agaaagaatt tctctttcac
     4801 taagcccaaa agaagagcaa caactcacag attcagcggc atttattcga aattatctta
     4861 agcatatcaa tctttaaaga gtatctattt aataaaaagt catataatcc aaaaaaagct
     4921 caccataagt gagcttttaa ctattatctg ttttatgtta attatgcttt gtttgctgaa
     4981 ccaaacacgt caatacgttc ttcaactgct tctgtaattg catcgaaacc aggtttcaag
     5041 aatttacgtg ggtcaaagag ttttttcttc atgtattcag cttcgttagc gttgaattca
     5101 acagcgtagt cacgagttgc ttgacagaaa gctaattgac attctgtgtt aacgttaact
     5161 ttagcaacac caagtttgat agcttcttgg atttgatcat caggaatacc tgaaccaccg
     5221 tgtaacacga ttgggaaacc aggaacagct tcagtcaatt tcttcaagtg atcaaggtca
     5281 agtccttccc agtttgctgg gtatggtcca tgaatgttac cgatacctgc agccaagaag
     5341 tcaataccag tttcaaccat agctttagcg tcttcgattg gtgctaattc acctttaccg
     5401 acaataccat cttcttcacc accgatagta ccaacttcag cttctactga aataccttta
     5461 gcatgtgctt tagcaacaac ttcgcgtgct ttttcaaggt tttcttcaac tggaaggtgt
     5521 gaaccatcaa acataattga agtgtaacct acttcaatac actcaagggc atcatcgtaa
     5581 tgaccgtggt caaggtggat agcaactggt acagtaatac ccattgattc aaccaaagtt
     5641 tcaataagtt gtttacaaag tttgtaacca cccatgtatt tagccgcacc cattgaagtt
     5701 tggataagta ctggagcttt tttagcttct gctgcgcgca agatagcttg agtccactca
     5761 aggttgtttg tgttaaatcc acctactgca tagccgttat cacgagctgc ttggacgaat
     5821 ttttctgctg aaacgattgc cattattcag gcctcctatt tattttttgg gcgttgccca
     5881 cttacatgtt aattctagca caattctata aaaaatgcta gttttttact gaaaatacgt
     5941 gagaaactta acagagttaa acgaggtttt ttcagaaagc agtcattccg ctttcaattc
     6001 tttacgtgcc aacaggatct gttctttaac ctggtcaaac cctgttcctc ctaatgaatt
     6061 tcttcgttta actgctgtct ttgcagttaa tatctcatag atatcgtttt ctatcaactc
     6121 tgaaatttct tgataatatt ttaaaggtat atcctgtaaa taagaaccat ttttactgca
     6181 ttctaaaacc aatttaccta caatttcatg tgcttttctg aaaggtaccc ccttacttgc
     6241 taaataatct gctaattctg ttgcatttga aaaatcagtc tccgttgagg tcatcattat
     6301 gtgctcattc acagtcattt tctcaagcat attagccatg atttcaatag ctatgctgac
     6361 tgtttctacg ctatcaaaca tcccttcttt atcttcttgt aaatctttgt tataagctaa
     6421 cggcaatgat ttcataacag ttagtaaact aaataaattt ccataaacac ggcctgtctt
     6481 ccctcgaatt aattctgcca tatcaggatt tttcttttga ggcataatag atgatcctgt
     6541 agagaaagta tctgataatg tgatgaattg atattcataa ctgcaccaat taatgatttc
     6601 ttcacaaaaa cgtgataaat gcatcattaa aatgctagca ttggataaaa attctatgat
     6661 aaagtcacga tctgagacag catcaagcga attactatat ggtttttcaa aatctaataa
     6721 acgagttgtc atatggcggt caataggaaa tgttgtccct gctagagctg ctgctcctaa
     6781 tggtgataga ttggtgtgtt tcatattaaa ctctaatcgc tctgtatcac gcgtaaacat
     6841 attatagtaa gccattagat gatgtccaaa tgaaatcggc tgagcatgtt gtaaatgtgt
     6901 atatcctggc ataacagtat agatatgatt ttctgctaaa ttaactaagg ttgttcgtaa
     6961 atgaagcaat ttcttcatca tttcttgcaa tttatctttc aaataaaggt gcatatctgt
     7021 agccacttga tcatttcgtg aacgtgcagt atgaagctta cctgctactt ctccaatttc
     7081 agcggttaat aagctctcta tgttcatatg aatgtcttca tttgatatgc taaaagtcaa
     7141 ttgacctgct ctgtatttat cttgcagtat ttttaaacca tcttttatca agcctgcttc
     7201 ttcttgacta ataatatccg ttttgcctaa cattgtgaca tgggccatag aagccttcat
     7261 atcataggga gctaactttt ggtcgaaact gattgatgca ccgaactctt caacccattt
     7321 ttcaagacta ctttcaaaac gaccacccca taacttatga ttttttgtca tcctcttatc
     7381 ttcctttatt tatcctttgt ttacttgtgc attaacttgg gttggaagtc cccagagttt
     7441 gataaaacca actgctgcat cttgatcaaa gttatcagca gctgtataag ttgctaagtt
     7501 ctcatcatat aatgaattag acgagtggcg agcaacgact ttggcagaac ctttatataa
     7561 cttaactttt gtagtaccat taaccacttt ttgtgtttct ttgacatagg caatgatagc
     7621 ttttgtagct ggactaaacc ataaagcatt ataaataaga tttgatagtt cattttcaag
     7681 gataggttta aaatgagaca cttcacggac taatgttaaa tcttcaattt ctttatgagc
     7741 agctaacagt accattgccg caggacattc ataaatttca cgtgatttga taccgactaa
     7801 acgattttca acatgatcaa tgcgtccaat accatgtttt cctgcaattt catttagcga
     7861 taaaatcaaa tctgctagtg tcatttcttg attgtttata gctataggct tcccattttg
     7921 gaacgtaata tcaatatatt ctgcacaatc aggagcttct tctggtgatt ttgtaatacc
     7981 aaaagcttct tctggcgctt gattccaagg attctcaagc acaccacatt cattggcacg
     8041 tccccataaa ttttggtcta tagaataagg gttatctaaa tctgctggaa taggtacgcc
     8101 gttagctttt gcaaaagtaa tttcctcttc acgatgccac tgccactcac gaacaggggc
     8161 aattactttt aattcaggat ctagtgctgc aattgcaact tcaaaacgca cttggtcatt
     8221 tcctttacct gtacatccat gtgcaattgt ggtcgcacct gtttgatgtg ccatttcaac
     8281 taatttttgg gcaataattg gacgactaag cgctgaaaca agcgggtatt tttgttcata
     8341 catcgcatgt gcctgtaaag ctggtaaaac aaaatgctca gcgaactcat ctttaacatc
     8401 taaaatataa gattctatgg cacctattgt taaagcttta tcgtgaataa aatcaagatc
     8461 tttaccttcg ccaacatcca tacagacagc aataacgtca taatcttttt ttaaccaagc
     8521 tatagcaaca gaggtatcta aaccaccaga ataagctaaa attaattttt cttttcccat
     8581 aaaaaatccc cttccaaatt cctatatatt gtaatattat acacttgcta tatttattat
     8641 caaatatttt tatagttttt gtctattttt agtatattta tttctaaaca aataaaacaa
     8701 taactgccaa ttggcagtta ttgttttaaa taattatgat aaaggtagtt ctacacaaaa
     8761 ttctgttcca atttccaact gactggagac actaatatgt cccttacaaa gaacaattat
     8821 acgtttaact attgctagtc ctaatccatt tccttgttga ttatgcgact gatccgcttg
     8881 gtaaaattta tcaaatatat gctgaacatc atattgagaa attcccctac ctttatcaga
     8941 aataataact ctcagataat gattattagt ttcttccatt cgtacactta agtctacaat
     9001 atcctcggaa tattttattg cattatctaa aaggtttatc caaacttgca tcagcaagtc
     9061 agagtttgag taaacagtat atggttttga atccagttga aaatttatcc ttttagcttg
     9121 ccaacgttct gttaaactaa tcattgcttg acgaatttgt tcatctacac gaacagagga
     9181 tagctcccca atagtctcct gattatccac gcgcgacatt tgcaacatcg tatcacataa
     9241 tcttgttaac cttaggctct ctgttttaac aagacctaat aactcacttt gtgtttcttc
     9301 atctaattct ggatcttgta atagttctac aatattagct actgcagcaa tcggagtctt
     9361 tagctcatga gaaacattag tgataaagtc ttttcgcata tgatccatat gagataaaga
     9421 aacaatcatc tgattaatac tttcagatag ctcatccaat tcattataat actcatagga
     9481 tttacgacga ttactgtttc gataaatatg cccttcaaag tcaccgcctg caacattgga
     9541 cacaatatgg ctaatatcta aaattggttt tgttaaatga taagaaccgt accacatact
     9601 aatactagct atcacaaggc ttaaaaaaac aacagcgaga gcgctaactt gtaatacatg
     9661 agtagttaag actccttgaa aaaaagtaaa aactattgct gcaactagta aaagagaagc
     9721 taggactgag aaaagcatcg ttaacacaaa cattaatgtg aaataaaatc ttaacggaaa
     9781 ccgaaatcgc cttagcttag tcattttttg cacgataccc tacccctctc accgtttcaa
     9841 tcttaaaatc tggtaagtgt tctacttttc gtctaatttt attgatgcaa gcatctacca
     9901 cccgctcatc taaatcagta tccattcccc aaatactatc caatagttct aaacgtgtga
     9961 aaatcctatt aggatagcta agtaagtgga aaagaaccct aaattctttc tgaggaaata
    10021 caatttcttg ctcaaaatat ttcaagctta attcatcttc attaagacaa gtattcccaa
    10081 taattagctg atgttgagct acaatattag ctctcctcaa gagtgcctta atacgtaaag
    10141 ctaattcttg taactgcaca ggcttaacca tataatcatc tgcaccaagg ccaaaccctt
    10201 ttgtcatatc ttccatttgt gattttgctg tcataacaat gattgggaca ttgtaagcag
    10261 ctgacctgag ttcttgaatt aattgataac catccatcat gggcatcatg atatcagtta
    10321 taatcaattg tacttcttct gtatctagac aatttagagc ttcttgtcca tttgaagcag
    10381 tgataaattg gtagccctct tgtgttaatt ttgtggtaat gattttttgt tgaacaatgt
    10441 tatcctctac aactaatatt ttaatcatta aatcacctct agcttattgt aacacaagtt
    10501 agaagttttc tttaactaat ttcccgtcct ttaaacgata aacggtatct actaaatcta
    10561 gaacgcgctc atcgtgtgtc accataatag ctgcagtatt atgtgattta acttcctgac
    10621 gaataagttc tgtaacttga cgcccacgtt cttcatcaag gctagctgtt ggctcatctg
    10681 ccaagataac cttaggatga ttaataaatg ctctagcaat tgctgcgcgt tgcttttgtc
    10741 ctcccgataa ttggttaggg tatttatgtg cacactgctc tattcctaaa tctgataata
    10801 atttattaat ttcttccaag cttgttttct tatcatagtg ttttgcaaat cgagcctctt
    10861 gttgcaactg ttcttggatt gttaaataag gaagcagttg atgactttga aatataaaac
    10921 ctagtgcttc acggcgcaat tgtgttcttt gtctttgaga caaatccgta acgttctcat
    10981 gattgacata caaactacct tgatctgcac caagaagtaa ccctgcaatg gataaaagtg
    11041 tgcttttacc tgaacctgag ggtcccagta tagctacaaa ctcacctgca gaaactgata
    11101 aatctaaatt atctaatatt gataaaagct catctccatc tggatagtgt tttgagatgt
    11161 gttttaattc taagattgct gacattattc tcctccccca ttaataactt cgactgggtc
    11221 gattttagca acttttacaa ttgacaaagc actactaatc attgcaatga ctaaaaatac
    11281 aaacgatact agaataatat tttgccaatt aataacaaat ggcatttgag ctggtaatac
    11341 ataagagaga gcaatcgcca atcgatctcc aacaatgata ccaaacagtg ccaatatgat
    11401 gacttgactc aattgaaata gggcaatctc tgacattttt gtcccaatag ctttcatgac
    11461 gctaaattca tgacgttttt gaagtgtgat gatgtagaaa aagacaccta aaataccagc
    11521 tgaagctacg acaaggaccc ataaaatcat attcaaagtg gattgttcag cactataacc
    11581 aggaatatgt tgaatgacat cttttttact aacagcttct aattgagttg gtaaatgaga
    11641 tatactttta tcatttttca taactaatgc ctgaggtaag aactggtatt gaggattaat
    11701 ctttttatta atttcggtat aaatgtcttt atcaataaat gccacaggtc cgtgaccata
    11761 catactatta tggacaaatc caacgactgt tagtgatata gatgatgact tatcaatcac
    11821 tttatcacct aactttatcc cttcagcttt taaactatca tttaaaataa tagctttttt
    11881 gtaacttgtc agttgttttc cctcagaaag tgtaggtgcc ataaaagaat ctttatcaat
    11941 agcaaaataa gaaatatcaa ttttcttttc atgtccttgt cgagttaaac tcgaacgttg
    12001 aatatttaaa gtggtactat cttttaaatt caaactgttt aaatctgtct gatctttagt
    12061 tgtcaataca gatgatgtaa tcacttgttc agcaccttca ttcaaaatat aggtttgagc
    12121 tgggttattt tcaatagcag cactaacagc tctacctaaa ccgtttgcta aacctgataa
    12181 aaaaactacc atgaacatca taacaatgat tagcaattca atcaggaggt attttttctt
    12241 ataaaaggtt aattctttcc aagcatgtga catggttttt ctcctttgat cttttgatgt
    12301 gaacatagta tcacagcttt atgacttgaa tatgacctct aaaaataatg atattttatc
    12361 attaaattat ttaaaaaata gcttgacaat tttttagtaa gcgattacaa ttctaattat
    12421 gaaaccgttc ccacaataaa agaaaggagt ttctatgagt acgattagac aagtcgccga
    12481 gaaagctggc gtatcaacat ctactgtctc aaggtatata tcccaaaatg gttacgttag
    12541 tcaaaaagca agtcaaaaaa tcgagcaagc tatccgcgaa ctccattatg ttccaaattt
    12601 ccttgcacaa tctctgaaaa ccaaaaaaaa tcaacttgtt ggattactac ttcccgatat
    12661 ttcaaaccct ttctttccaa gacttgctag aggagttgaa gaatttctaa aagaacaggg
    12721 ttatcgtgtc atgcttggca acacaaataa caagagccat ttagaagaag agtacttaaa
    12781 tgtgctatta caaagtaatg ctgcagggat tattacaaca cacgatttca ccaaaaatca
    12841 tcctgaaata gatattccag ttgtagttgt tgaccgcgtt aatcaagaaa cacagtacgg
    12901 cgttttctct gataataaag agggaggaaa attagcagct caagctattt ggacagcagg
    12961 tgctaccaat atattactca ttcgtggtcc gctagataaa gctgataacc ttaatcaaag
    13021 gtttcaagga agccaaaatt acctattaaa caaaggggct cgttttgcta tcgaagatag
    13081 tgccagtttt gattttgcag agattcaaat tgaagcgaaa actttacttg accaccatcc
    13141 agatattgat agcattatcg ctccatctga tatacatgct attgcctacc tacacgagat
    13201 tcttaatcga gggaagcgaa tccctgaaga tgtacaaatt attggttatg atgatatttt
    13261 aatgagccaa tttatctacc cttcgttgtc aactattcac caatcatcat atattatggg
    13321 acaaaaagca gctgaattaa ttttcaaaat tactaatcaa ttacctatta ccaataagcg
    13381 tattaaatta cccgttcatt atgtcgaacg tgaaacccta aggaggaaaa tagatgagta
    13441 acattgtcat tatcggtagt atttccatgg acttagttat ggaaaccaat cgtatcgcca
    13501 aagagggaga aaccgtattt ggccaacgtt tttcaatggt tccaggaggt aaaggtgcta
    13561 atcaggctgt cgctattggg cgtcttagcc aagatcgaga taatattact attcttggtg
    13621 ccattggcga ggattctttt ggtcccattc ttttggataa tttaaacaaa aaccatgtta
    13681 ctacggattt tgtgggaacg ataccatctt cttcaggtgt tgcccaaatc actctttata
    13741 ataatgataa tcgaatcatt tactgtcctg gagccaacgg aaaagttgat actaaaaagt
    13801 ggtctcagga gtggtcaata attaaagaag ctgatttagt tgtattacaa aatgaaattc
    13861 ctcatcaagc aaatatgaaa attgctaatt tttgtaaaga acatagtatt aaattactct
    13921 ataatcctgc accatctaga aagactgata ttgagatgct cgataaggtc gattatttca
    13981 caccaaatga acacgaatgt caagagctgt tcccaaacca aaaactagag gatatattag
    14041 ccacatatcc tgaaaaacta attgtaacac tcggcacaaa aggagccatc tactctgatg
    14101 gaaaagaaag ccatttaatt cctgccttag aaactaaagc tgttgataca actggtgcag
    14161 gtgatacttt taacggtgca ttcggttatg ctatctcgaa aaaatttaag attgctaatg
    14221 ccttacgttt tgcaactcta gctgctcact tatccgtgca aaaatttggt gcacagggtg
    14281 gcatgcctac aattaaagaa atggaggaca accaacatta tgaaaaagac tggtatatta
    14341 aatagtcatc tcgctaaatt agcagatgac cttgggcata ctgatcaagt ttgtattgga
    14401 gatctgggtc tacctgttcc aaatggcatt ccaaagattg atttgtcttt gacctctgga
    14461 atacctagtt tccaagaggt acttgatatc tatttagaga atatcttagt tgaaaaagtc
    14521 attttagcag aagaaatcaa ggaagcaaat tcggatcaat tatcaaggtt attagctaaa
    14581 cttgataact ctgttaacat agagtacgtc agtcacaatc atttaaaaca aatgacccaa
    14641 gatgtcaaag cagttattcg tacaggtgaa aatacaccgt attcaaatat tattctacaa
    14701 tctggtgtta tcatctagaa aggagtttag tccatgaaaa ttgacatgag gaatatctcc
    14761 aaatccttcg gtactaataa agttctagag aagattgatt tggaattaca atctggtcaa
    14821 atacacgcac ttatgggtga aaatggtgcg ggtaaatcaa ccttaatgaa cattctaaca
    14881 ggactttttc cagcaagtac aggaactatt tatattgatg gcgaggaacg aactttttca
    14941 aaccctcaag aagcagagga atttggtatt agttttattc accaagagat gaatacttgg
    15001 ccggaaatga ctgtcttgga aaatctcttt cttggacgtg aaatcaagac aacttttggc
    15061 ttactcaatc aaaaattaat gagacaaaaa gcacttgaag cttttaaaag attaggtgtg
    15121 actattcccc tagacatccc gattggtaac ttgtctgttg gacaacaaca aatgattgaa
    15181 atcgccaaaa gtctcctaaa ccaactctct attttggtaa tggatgaacc gactgctgct
    15241 ttaacagatc gtgaaactga aaatcttttc cgagttatcc gaggtttaaa acaagaagga
    15301 gtgggcgttg tctatatctc ccatcgaatg gaggaaattt ttaaaatcac tgacttcgta
    15361 accgtcatga gagatggtgt cattgtagat acaaaagaaa catcactcac aaattctgac
    15421 gaattggtta aaaaaatggt tggccgaaaa ttggaagatt actatcctga aaaacattct
    15481 gaaattgggc ctgttgcttt tgaagttagt aacctctgtg gagataattt cgaggacgtt
    15541 tccttttatg tccgtaaagg cgagattctt ggattttctg gattaatggg agctgggcgt
    15601 actgaagtta tgcgaactat ctttggaatt gataaaaaga agtctggtaa ggtgaaaatt
    15661 gataaccaag aaatcactat tactagccca agccaagcta tcaagcaggg cattggtttt
    15721 ttgaccgaaa atagaaaaga tgaaggtctc atcctagact tcaatatcaa ggacaatatg
    15781 actctcccta gcactaaaga ttttagcaaa catggtttct ttgatgaaaa aacaagcaca
    15841 acatttgtgc aacaattgat taatcgcctc tacattaaat cagggagacc agatttagag
    15901 gttggaaatc tttctggagg taaccaacaa aaggtcgttc tcgctaaatg gattggtatt
    15961 gctcctaaag tcttaattct tgatgagcca acacgtggtg ttgatgtcgg agctaaacga
    16021 gagatttacc aattaatgaa tgaactagca gacagaggtg ttccaattgt catggtatca
    16081 tcagacttac ctgaaattct aggtgttagt gaccgcatca tggtaatgca cgaaggtcga
    16141 atcagtggag aactcagtcg aaaagaagct gaccaagaaa aagttatgca gctagccaca
    16201 ggaggtaagt aaagtgaaaa atatgatgaa atatatatca gagttaacaa ccctgattgc
    16261 attagctggt ttaatggtta ttatcacact cattaatcct aatttcctca caaccaataa
    16321 tctactcaat ttattattac aagtaactgc aaatggtttt atcgctttcg ggatgacctt
    16381 cgttattttg actggcggta ttgatttatc agtcgggtcc atattagccc tatctagtgc
    16441 attaacagct ggattaatcg gccacggagt acccgttggt attgctatcg ttttatctgt
    16501 tgccttaggt ggcatcttgg gaatgctaaa cggactcttc atttcttacg gaaaattagc
    16561 accatttatc gttacactgg caacaatgac tatctttaga ggagcaacgc tagtttattc
    16621 aaacggcaac ccaattaccg ctggattatc cgatagtttt ctattccaat ttttgggaca
    16681 aggctatatt gttggtatcc cattcccagt cattctcatg tttcttactt ttattatttt
    16741 gtatattttg ttacataaaa cagccttcgg gaaatctgtt tacgctttag gaggtaatga
    16801 aaaagcagcg tatatctcgg gtattaagtt aaacaaagtt aaaattatca tctacactat
    16861 ctcaggtatc atggcttcca tttctgggtt aattatcaca tcacgcctta gctcagcaca
    16921 accaacagct ggagcaagtt atgagatgga tgctattgcc gccgttgtct taggtggaac
    16981 gtctttatct ggtgggaaag gacgtatcat tggaacattg attggtgctc tcattattgg
    17041 tgtcctgaac aatggattaa acatcattgg tgtctctgca ttttggcaac aagttgtgaa
    17101 aggtatcgtt attttaatgg ctgttttact tgatcgcttt aaagtagcaa aatagtcttt
    17161 actctaattt ctataagttc attatatttt atctaaagga gtttcaaatg aaatttggaa
    17221 aaaaacttgg ctttctagcc ttattgatgt ctattgtcct cattctcggt gcatgtggta
    17281 aaactggact tggaaattct tctgggaatt ctacaaaaaa tgtaaccaaa aaatctgcta
    17341 aagatttgaa actaggtgta tctatttcaa caactaacaa cccctacttt gttgcgatga
    17401 aagacggtat tgacaaatat gcgagcaata aaaaaataag cattaaggta gctgatgctc
    17461 aagatgacgc cgcacgtcaa gcagatgatg ttcaaaactt tattagtcaa aacgtagatg
    17521 ctattttgat taatcccgtt gactcaaaag ctattgtaac agctattaag tctgcaaaca
    17581 acgctaatat tcctgttatt ctaatggacc gtggtagtga gggaggcaaa gtattaacta
    17641 ctgtcgcttc agataacgtt gctgctggta agatggctgc ggattatgcc gttaaaaaac
    17701 taggcaaaaa agcaaaagcc tttgaattat ctggagtacc aggtgcctct gctactgtag
    17761 accgtggtaa aggatttcat tcagttgcaa aatctaaact tgacattctt tcaagccaat
    17821 ctgccaattt tgaccgcgcc aaagctttga atactacaca aaatatgatt caaggacata
    17881 aagatgtcca aattattttc gcacaaaatg atgaaatggc acttggggct gcacaggctg
    17941 tcaaatctgc tggcttgcaa aatgttctta tcgtaggaat tgatggtcag ccagatgccc
    18001 atgatgccat taaaaaaggt gatatttcag ccactattgc acaacaacca gctaagatgg
    18061 gagaaatagc tattcaagca gcaattgatc attataaagg caaaaaagta gagaaagaaa
    18121 cgatttctcc aatttacctt gttaccaaag acaatgttga aaaatacaat tggtaaaagt
    18181 acacaaaaag tcgtgaaaat tcacgacttt ttatattata attttgctaa catattatcg
    18241 atatgttgaa ctgatttttc tttacctaat aaataaatag tatctggtaa ctctggtcca
    18301 tgcatttcac cagaaaccgc aatacgaata ggcataaata ggttcttacc tttgatgcct
    18361 gtttcttttt gaacagcctt aatttgaggg aaaatagtat ctctagtgaa ttcttcgtca
    18421 gaaagcgata ctaacttttc cttaaaagca gataagactg ttgggactgt ttctgctgcc
    18481 attacttctt tttcggcttc tgttaattct ggaaaatctg caaagaaaag gtctgttaat
    18541 ggtacaatct catcagccga ttttaattga ggtttataaa gctcaactaa tttttcagct
    18601 ttgtctgtaa gtcgaccagc ttcttctaag aatggtttgc aaagtgcaaa aacactctca
    18661 aaatcagcat tttttaggta atcattactc atccaatcca tcttcttctg atcgaatgct
    18721 gctggtgatt tgctaagacg attttcatca aatagattaa ttaattgctc gcgtgagaag
    18781 atttcttctt ctccaccagg attccaacca agaagggcaa tgaaattaaa gacagcttcc
    18841 gacatataac cttttttacg atagtcttcg ataaattgaa gagtatttgt gtcgcgtttt
    18901 gataatttct taccagtctc tgagtttata atcaatgtca tgtgaccaaa ttgtggcgct
    18961 tcccaaccga gtgcttcata aaccattaat tgttttggcg tatttgcaat atggtcatcg
    19021 ccacgaataa catgcgaaat ttgcatgtca tgatcatcaa tgactactgc gaaattgtaa
    19081 gttggataac catctttctt ctgaataacc cagtctccgc cgatattaga gccttcaaac
    19141 tcaatgtcac cttttaccat atcagtccac ttataaatcc cagattcatt aacagcaagg
    19201 cgtactgtcg gaatgatgcc tgctgcttca cgttctgcaa tgtaagcttc tttttcagtt
    19261 tctgacattc cgataaattc gttgatatag cgaggtgttt cgcctgccaa ttcttggcgt
    19321 tcacgctctg ctgctaattc ttcctcagtc acataggact tgtaggcttt accttctgct
    19381 aacaactgat caatataacg ttggtagagc tcaaggcgtt ctgattgacg ataattttca
    19441 tgtgtttcag ggctttcatc ccagtccata cctagccaac gtaagttctc caattgagag
    19501 cgttcaccat cttcgacgtg acgttttcgg tcagtatctt caatacgaat aacaaaatcc
    19561 ccaccatgat ggcgagcata aaggtagtta aagagcgcag tacgcgcatt tccgatgtgt
    19621 aacaatcctg ttggacttgg tgcataacgc acacgaatct tattagccaa aattctactc
    19681 ctatattttc tgatagtaaa ctttttcagt ttaactgaat tagtcaaggg ggcaaaacat
    19741 cattcaaact cttacagatg attgccaatc aaccctatta taccactaaa tcactaaaaa
    19801 tgataatcag taataatagt cacactaatt ttcgataaa
//
LOCUS       DATRME010000033        19570 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRME010000033 DATRME010000000
VERSION     DATRME010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19570
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            100..2601
                     /locus_tag="VBC23_001162"
     CDS             100..2601
                     /locus_tag="VBC23_001162"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6843113.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(2691..3299)
                     /locus_tag="VBC23_001163"
     CDS             complement(2691..3299)
                     /locus_tag="VBC23_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6843114.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(3392..4780)
                     /locus_tag="VBC23_001164"
     CDS             complement(3392..4780)
                     /locus_tag="VBC23_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6843115.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDKFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(4777..5462)
                     /locus_tag="VBC23_001165"
                     /pseudo
     CDS             complement(4777..5462)
                     /locus_tag="VBC23_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5695..10410)
                     /locus_tag="VBC23_001166"
     CDS             complement(5695..10410)
                     /locus_tag="VBC23_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6843116.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISP
                     NDDQNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     QSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLRVTSQTDNQKIKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            10733..11425
                     /locus_tag="VBC23_001167"
     CDS             10733..11425
                     /locus_tag="VBC23_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6843117.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            11415..11738
                     /locus_tag="VBC23_001168"
     CDS             11415..11738
                     /locus_tag="VBC23_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6843118.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            12108..14345
                     /gene="metE"
                     /locus_tag="VBC23_001169"
     CDS             12108..14345
                     /gene="metE"
                     /locus_tag="VBC23_001169"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6843119.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            14390..16234
                     /locus_tag="VBC23_001170"
     CDS             14390..16234
                     /locus_tag="VBC23_001170"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6843120.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            16581..17288
                     /locus_tag="VBC23_001171"
     CDS             16581..17288
                     /locus_tag="VBC23_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6843121.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(17453..18538)
                     /locus_tag="VBC23_001172"
     CDS             complement(17453..18538)
                     /locus_tag="VBC23_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6843122.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(18739..19278)
                     /locus_tag="VBC23_001173"
     CDS             complement(18739..19278)
                     /locus_tag="VBC23_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6843123.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         5885 a         3329 c         3756 g         6600 t
ORIGIN      
        1 tggggagaga acctgctgtt gagggtattt tcaccttttt tattgctgtt tttcgtgtta
       61 taataagagg actatacttt tttgacatgg agaagtgcga tgacattcta taatcacaaa
      121 gaaatcgaac ccaaatggca agctttttgg gcagataatc acacctttaa aacaggaaca
      181 gacgcatcaa aacctaagtt ttatgcccta gatatgttcc catacccatc tggagctggt
      241 ctccacgttg gtcacccaga aggctataca gcaacggata ttctcagccg tttcaaacgt
      301 gcccaaggtc acaatgtcct ccacccaatg ggttgggatg cttttggact tcctgctgag
      361 cagtacgcta tggatactgg taatgaccca gctgagttta cagctgaaaa catcgctaac
      421 ttcaaacgcc agattaatgc tcttggtttc tcatacgact gggatcgtga agttaacaca
      481 actgacccta actactacaa gtggacacag tggatcttta ccaagcttta tgaaaaaggt
      541 ctagcttacg aagctgaagt tccagttaac tgggttgaag aattgggaac agctatcgcc
      601 aacgaagagg ttcttccaga tggtacatca gaacgtggtg gctacccagt tgtcagaaaa
      661 ccaatgcgcc aatggatgct caaaatcact gcctatgcag agcgcttgct agaagacttg
      721 gaagaagttg actggccaga atcaatcaaa gacatgcaac gcaactggat tggtaaatca
      781 actggtgcta atgtaacctt caaggttaag gatacagata aggacttcac tgtctttaca
      841 acacgtcctg acaccctttt tggtgcaact tacgcagttc ttgctcctga gcatgccttg
      901 gttgatgcta ttacaacagc tgaccaagca gaagccgttg ccgaatacaa acgtcaagca
      961 agtctcaaat cagaccttgc tcgtacagac cttgccaaag aaaaaacagg tgtctggaca
     1021 ggtgcctatg ccattaaccc agttaatggt aaagaaattc cagtttggat tgcggactat
     1081 gtccttgcca gctacggtac aggtgctatc atggctgttc cagcccacga tgaacgtgac
     1141 tgggaattcg caaaacaatt taagctagac atcattccag ttcttgaagg tggtaatgtt
     1201 gaagaggctg cctttacaga agacggtctt cacatcaact cagacttcct agatggtctc
     1261 gataaagcgt cagctattgc caaaatggtc gaatggcttg aagcagaagg tgttggtaat
     1321 gagaaagtca cttaccgtct ccgcgactgg ctcttcagcc gtcaacgtta ctggggtgag
     1381 ccaatcccaa tcatccactg ggaagatggt acatcaacag cggttcctga gagtgaattg
     1441 ccattggttc ttccagtaac taaggatatc cgtccttcag gtactggtga gagcccactt
     1501 gctaacttga cagactggtt ggaagtgact cgtgaagatg gtgttaaagg tcgtcgtgag
     1561 actaacacca tgccacaatg ggcaggttca agctggtact atctccgtta catcgatcca
     1621 cacaatactg aaaaattggc tgacgaggaa ctcctcaaac aatggttgcc agttgatatc
     1681 tatgtcggtg gtgcagagca cgccgtgctt caccttctct acgctcgctt ctggcacaag
     1741 gttctttacg atctcggtgt cgtacctacc aaagagccat tccaaaaact ctttaaccaa
     1801 gggatgattt tgggaacaag ctaccgcgat agccgtggtg ctcttgtggc aactgataag
     1861 gttgaaaaac gtgatggtag cttcttccat gtggaaactg gggaagagct tgaacaagca
     1921 ccagctaaga tgtctaaatc acttaagaac gttgtcaacc cagatgatgt tgttgaacag
     1981 tacggtgctg acacccttcg tgtttacgag atgttcatgg gaccacttga cgcttcaatc
     2041 gcttggtctg aagaaggtct tgaaggctca cgtaagttct tggatcgtgt ttaccgtttg
     2101 atcacgacta aagagattac tgaagaaaat agcggagccc ttgacaaggt ttacaacgaa
     2161 accgttaagg cagttactga gcaagttgac caaatgaaat tcaacacagc tatcgctcaa
     2221 ctcatggtct ttgtcaatgc agccaacaag gaagacaaac tctttagcga ctatgccaaa
     2281 ggctttgttc aattgattgc accattcgca ccacacttgg gtgaggaact ctggcaagtc
     2341 ttgacagcct caggtgagtc aatctcttac gtaccatggc caagttatga cgaaagcaaa
     2401 ttggttgaaa acgaaattga aatcgttgtc caaatcaaag gtaaggtcaa agctaaactt
     2461 gtagttgcta aagacctttc acgcgaagaa ttacaagaag ttgcccttgc taacgaaaaa
     2521 gtccaagccg aaatcgcagg caaagacatc atcaaagtca tcgcagtacc aaacaaactg
     2581 gttaatattg tggtgaaata agataaagat tttactagaa atagtgatat ttacgcgtat
     2641 aaaatcccaa aagtttatga acttttggga tttttctatt tttagctaaa tcagtcagct
     2701 cgtactgttt ttttagcata gtaagttttt aatacctctt ttgtaatacc atgtttttga
     2761 tagatttttt ctatttttga cgttaatatt tttagttctt tattttggtt ttcaatagag
     2821 ttatcaaatt tttgatagcg gtcgttcaac cttgtctcaa ggtaagcacg ttgttcaaaa
     2881 tataatttta atctatcttt ttcgttagtg gatagggttg tcttatcaat caattttttg
     2941 acttcacttg tgctatccca tgtttgatta tcaatagaag tatagaattt agttagtaga
     3001 cgtttatttt gcttctctag ttggttgata tctttgttaa tatacaattc aataactttt
     3061 aaatcttttg cttttttgac ttcaatatgt cgttggatat ggtcgatttt agcgtagaga
     3121 ggagcagctt ctttatctgc aacttttaac ttatgttcaa tagttttttg actcatatga
     3181 acagttgatg ttttttgtga ttgttcagcc gctacatgat ttggttgtaa actcgtatat
     3241 gttgctaaag agagaacggc aataagcgat aaggaatgta tttttttaga atgtaacata
     3301 taaaaactca ctttctttta gaataacttt ttgatctata ggtattatag gttaaaaaag
     3361 caataacttc tttgaatttt tgtaggaaaa gttaggcaat tttgtggcaa ttgaaataaa
     3421 tcgtaaaagt tgttgaggtc gaatcactct cagcgatgat attaccatga tgttgcttga
     3481 taatttcttt agtcattgca agtccaatac ctgtttggcc gccatgtcct ttatagaagc
     3541 gatcgaaaat ataaggcaag tcttcttttg aaatggctgg tccgtcgttt gagatagtta
     3601 gtttttgatt tgtgtaagag atttcaatat gagaaacagc atagcgtagt gcgttactaa
     3661 ggatattatt gatagctcgt tgaatcaact cttcgttacc ttttagtaac ttttcatgct
     3721 tgtctggtct ataagtgata ctaatctttt gtttattagc taggggtttg aaataagcaa
     3781 taatactatt aagtaattca tttaaagaaa atttatcttt ttgatcactt aagttactat
     3841 ctaatttaga gagtaagata atatcgtcta cgagctgttt catttttttg ctctcttgca
     3901 gaataacaga gtgtgcgagc tcggcatcaa taataccctc ttgaactccc tcagtatatc
     3961 cttggatgga cattaaaggt gtccgtaact cgtgggaggc attttgaaag aagtttcttt
     4021 ggctctcttc tgcctggtag attcgatttg ccatttcttg gctctcaagt agaagaggat
     4081 cgaaatcaga aaagacaatt tctttttgac tagattgatg attgccttga tttttccaaa
     4141 ggctggaaat ataagtttgt actgattgaa tagattctct aattccagtg aaagtcttcc
     4201 tcataataaa tagcatggga atataggtta gtactaagat aactataaga aaatgattaa
     4261 ttttagtaat tagatgctgg gtgtaagtaa tgtctgaaaa gttaatcaca tgaaaaagtt
     4321 gcgactgtgc tttaggagct ttttttatgc taccatcaac aaggatacca gagtagtctt
     4381 tggtcattac ataataggtt tttttcttga tggttataaa cataccatct ttttccttgg
     4441 aaatttgcca caatcttttt tgggtaagat agcgaataat atcatggctc tcttttttat
     4501 gattatcagt gccatatttc atacgataat gctggtcaaa aatagcatag taagaccaaa
     4561 caaagatgct atcttggtta accatatttt catctggtat tttcttgttg ttatagatac
     4621 tatctaaaat ggtgaaattc gcctgcatgg aattccttgt catttgatgg acatagtctt
     4681 taatgacata gttgacagaa aaatagatga ggtaaaaacc aagaaataga gtgatgctat
     4741 taataaggat aagattgcta attaaacttt tctttttcat ttgtcctctt tgattaattt
     4801 gaagccgtaa ccccagacag ttttgatact aacctgactg tggtattgct taagtttttt
     4861 acgtaatcgt ttaatagtgt catcaactgc tctgctttca atatcatcaa atccccaaat
     4921 acgattaagc agtgtttccc tagaaaaagc agtttcaggt ttttcaatta atactaaaag
     4981 aaaatcaaat tctgtcttgg ttaagggcat ttcttcattt gataataggg ctattcgctt
     5041 ttcagtatcc agaattaaat ctagaactga tactgagtgt catttttgat tgctttttca
     5101 tcaaggcgtt tgaacaatgc tttaacatgt agactaagtt ttataggtga aaaaggcttg
     5161 gtgaagtaat cgtcagttcc taaattaaaa gccgtaatga aatcaacatc agaatctcta
     5221 gctgttaaca tgataatagg aatattatca tattggcgga tgcttttcat aacatctaaa
     5281 ccattagttc caggcatcat tatgtctaat ataactaaat cgggtttttg gtttttatag
     5341 gcttctaaga gaaggtctcc agtttcaaat gctcgaacag tgaaaccatc atgttctaaa
     5401 aaaggaacca ccaagtcacg aatattcttt tcatcatcag cgaaataaat tatttttgtc
     5461 atgtatacct caatacccat agttataatt tgacttttag ctagctgttt atgttatatc
     5521 aaatataaca aaaaaagagt ttttttccta ttctttgaag tgttatagtg ttataaacca
     5581 agatattgca gagaaaaggt ctttaggaac atcattttaa gctcctcaaa aaggcaccag
     5641 tcactaacta ttaatagcta gtaattgatg ccttgctcta ttttttaaag tattttattc
     5701 tcttttgata gactttagtt tgattgttgc cagacctagc atagagagac taaatagcaa
     5761 tattgtatag gttattttag gagtgctttc gcctgtttta ggaagcttgt ttgtctttat
     5821 cttttgatta tcggtttggc tagtcaccct taagacacgc ttctgagcaa cctcactttc
     5881 tttagtcatg ccttttttga atatcaacgc ctggggctga tttggcggat tgataggtgt
     5941 ggtattgaga gagtcattat cattgagtga ttgactataa ttctctgcgg aagaaactct
     6001 atcgcgacca tcaagttctt gttcggttgc ttttatttgg tttaataact cttcaacttg
     6061 agcttgtgta gcttgtctat tggaaagcag caattctgct ttcttgataa ggttattata
     6121 attttcttta gcaaattgac tggcgttctg atatttagat gatgtttgtt tcagaacatt
     6181 tgctttaatt atcatatggt gtaaaccaga gtaatcagtt tcatgaccat ttaattgatt
     6241 tttagcaata tcaagatttt ctaaggcttt gtctacttct gattgagtga cgttaggttt
     6301 ttgtagaaga tttttcgcca attggacttg attgatataa gttgattggc ttaatggact
     6361 agcattaaca aattgatagt ttcccttgat ttctgtttca gctaaaatag cttttgccag
     6421 taaagaagta tctgtttctt taccgttaag agcttggcga ctttctctca aagcagcaat
     6481 agcattatca atacttgttt gttccactct atttttaatt aaagtttgtg ccttttcaag
     6541 attagctagg taattatttt ttaagtgagt tccagcatta taaaacacag gttgggaaat
     6601 aatgtcagtt tgttctgcta gggcattaat tagaggagct ttattaatca gcgtcaattt
     6661 tgttagattt acttgacctt cttttacgga tacaagtaaa tcatctaagt tctctaaagt
     6721 gctataccca ctaggcagcg ttacgtttaa acgatagtct cctacaggta tgtttttacc
     6781 atagtcagta ggagaataag ttgcttttgg tagatcaaca acagtatttg tcttagtaac
     6841 caactggact gttgctcctt ttggtaaaag cttgtcaaat tcaacgagta aggctgcttt
     6901 cttgagtaag ttaactttaa ataagacgtc tttaaggcta tctttttcag aaatagttac
     6961 agtgacactt tggggactga ttagatttgc tcgttcctca tcgtataaga ataggtcaaa
     7021 cgtataggta ccaaaaggca atttcagtaa attgaggtct ttcccgtgat gttgcttttt
     7081 gatgacatta cctttgtcat ctttgacaga gtaagagaaa tcaatatcga cattactgtt
     7141 aagctcaggg ctaaacactt tgacgtttac taaaccattt ttattgccaa tattgatcaa
     7201 atcttcgagt tttgctgaca ctagattgcc tgcataatct tcaacagtgt agtagacatg
     7261 agagaaatca ttaacctctt taggtagaat aaagctacca tccttttctt gtttgataaa
     7321 tactttgtta tcttcaacaa ggataccgtc ttctcctttt tgacgtaaga cgctattata
     7381 atgaccatct ttatcttttt ttaagtaaaa aacttgttct ctaaagatac cagattcccc
     7441 gtgttctact gcaggacgag ctttgaagat acgtctatct ttgtcataag ttgctgttgt
     7501 tagtgtagga gcttgtctat ccaaagtgat atcaaatacc atttgttgtg gcttagagcc
     7561 agggacatca gagtagtatg ttaaaacgta tttgtatttt ccatcttcta aaggaatacc
     7621 atcagtagtg gttcctttcc attctgtgtc atataaaaat gtagattttg gattttcagt
     7681 atttccgctg tagtaatttt tatctcctgc ttggggagca cttacccata gtggtttttg
     7741 taaattaaca tcatctgcac gatagacttt ggctcttaaa ttattgaaat tacgtaagaa
     7801 aacacctttc agtgcaacgg catcttgatt ttggtcatca ttaggtgaaa tggctagatg
     7861 aggctggcct ttttgctcta gttgtaagat ccattttcca tctatagact tatagcttcc
     7921 caaggctttc aattggatag gactagtacc gtctgttgag tagataggct ctgaacttgt
     7981 agtcattaag gcagtataat cttctgaacc aggaacttcg tttgtttgtt tcggttggaa
     8041 ataaaatccc ttttctttgt tagcaacaag cttataaatg gatttttcta aaacttctaa
     8101 gttttggaat tctcccttaa atccaacata aggaatacta agaacttccc caccatcaac
     8161 agggtctgta aaacgtacgt agccttctag gaaatagccg tttggcatta ctttttttaa
     8221 catgtcatgg tatttcgaaa catctatatc aattgtaatc gttttagttt gccctggttc
     8281 aatccgtatc gttttccctg taaatgtacc aagttgttga ggagccaatg tgacaaagcc
     8341 atctttaact tgatcagtat ttaaataggt tgtatagtgt aaatcttttg caactttatt
     8401 gatattatga acagtaacat caaaggaaat tttatcttta atatttccta atgtaacgct
     8461 accatagttg ttttccccac cagttaggta taatcctgtc tgaactgctg ctgcgacatc
     8521 gataatacct gctccttgtt ggcgtggtga ggtgtaagcg cctgtatctt tgtttaggtg
     8581 tgctttagca gtactcataa gaaggtattt gacagttctt tcaatgtcac cttttttaag
     8641 ttctggatgt tctttcaata agtattgttt aactagagca gtagcaccag cgacatgggg
     8701 agaagccata cttgtcccac tcatcatatc atattcatta tcattgatag cggcgtaaat
     8761 agagcctcca ggagcagata agtcaggttt taattgtccg tctgctgtta atccccagct
     8821 tgaaaagtca gataggacac ctgcattagg gttggcttgt ttatttttga tattgttaaa
     8881 tacaatttta tagttgtttt tagctaaaac atctccaaac tctttttgag taaaaatggc
     8941 aggaatagcg cttgcttcag gatctaaact cattgttaga ttagcttctc cagctttgtt
     9001 attaaagata atagctccca cagcaccgtg attaatagcg ttgacgactt tttttgtaaa
     9061 agtaatatct ccacgttcaa ttaaggcgat tttaccattt aaagttttgt ccttataatc
     9121 attgtcgttt ccttttccta cgaaaacata atcatattgc ttccctactt caaaggtctt
     9181 atcactaact ttaggatctg cataagcagc taatccgttg ttgagatttt tgttgttttc
     9241 taatccgata atgttgaaga ctttattaac cagtgttgta ttattatatg atgctacaga
     9301 aatggcctca cgagctgttg atggactacc aactaaacca taatcaggat aaagagcaga
     9361 aggctttgat gctccactcc caaatgtacc gtcgttacct gctgctataa caacagaaac
     9421 gccagcgagt ctagccatct ctaaagcttt tataagtcgg tcatcggcat taactaaaga
     9481 gccattagct ccacctaaac ttaaattaat gctatcagca ccgagtttaa cggcatcttc
     9541 aatagcttta acataaaggg ctggtccagt tcctcttttt tcatcagaga agactctcat
     9601 aaacattact tgtgcttcag gagcaacacc atagattaat tcatttgtat ctttcttgct
     9661 aggattagct gttgcgatac tggttacgtg cataccatga gaagtgcttt tcacctcttt
     9721 tagctctgta ttgacatcaa catagttgtg accaaagatt actttgttgt tataccattt
     9781 tccatagttt ataccagctt ttgctttagc agcattcatt tgctgttcgt tttggtattt
     9841 tgcttttgtc gaatcattta attggagagc atcgtgattg gtatctagtc ctgagtcaat
     9901 aattgctaca acagttccct ctcctttgta gcctgtgttc catactttgg ggacagtgat
     9961 aattttatta ctatcaatgt tttgaggggt ttctttagaa atagctggtg taggtttgac
    10021 tgctaaattt tgtttggtta aaggagtttt agaaacttct tgagtgcttt caaccggttg
    10081 atttgcggct ggggaaaatg tctcggtggc ttgagagtga ggctcagtta ctggttgtgt
    10141 ttctgttgga ctattattat tagcctttgt aacaagattg tcttgagctg ttgacgcaag
    10201 ggctgatgac gtgttgttag taggtgtctc atttgctatg ctcgttggac tgtcttttac
    10261 ttgctgttca actgcaatat cagatggctt atttatgaca gaatcagcag ctacatttgt
    10321 gataccgcct aaaaaaaata gggttcccaa aagtacagat acggcaccta acttatattt
    10381 ccggattgaa aaacgctgtt ttgtgttcat aaaaatctcc ttttcttgta acataataat
    10441 tacaaaaact attaaaacat aaaaaatcaa aattttctac aattttctga attttcatta
    10501 caaattattt tgtggtcaat ttaagaccgt acctaaagca ttttttaaaa atcattctat
    10561 gaaaataata atattgtcca actatatggc tacaatatca aaggtttata gaataattac
    10621 aggatattta ttagtctaat tgctggcaaa gctaaataat cattcctctt ctttcttagt
    10681 aatcaccgtt gtgctataat aactgaatat actgaaaatt tagggggata tgatggaaaa
    10741 aggatttaaa gagggagtga aagatgcctt gcccacagct ttagggtata tctcaatagg
    10801 cttggcgttt gggatagttg cttcagcatc agatttatct gcaatagaag ttggtcttat
    10861 gagtgccttg gtatatgggg gatctgctca gtttgctatg tgtgcgctat tattagcaaa
    10921 agcagacttg atgactatta cgatgacagt atttttagtt aatttgagaa atatgttaat
    10981 gagtttacac gcgactacta tcttcaagtc tgctcaccta atgaatcaat tagccatagg
    11041 aactctaata acagatgaga gctacggcgt tttacttgga gaagcattgc atcacaaagt
    11101 cgtatcgcct agttggatgc atggtaataa tgtgatgagt tatttgacat gggtaatctc
    11161 aacgataata ggaactttac tggggagtac tattcctaat ccagaaatgt ttggtttaga
    11221 ctttgcatta gtcgccatgt ttataggtct ctttgtattt cagttgtttg ggatgttatc
    11281 tgatggaaag cgacttgttg tatatgttct tgcttctgta ggtttaagtt acttcttatt
    11341 atccactttt ttgtcaggag ccttatcagt attgcttgcg actgttgttg gatgtagtgt
    11401 aggagttgtt ttagatgata aatagtaccg tttttcttgc tattctttta ggttttgttg
    11461 tttcttgggt gccaagagtc gctccattca tatttgtaaa atacaaaggg ctaccaaata
    11521 gtcttgtgaa atttttaaaa tttcttcctc tttctatttt atttgcccta acattctcta
    11581 gctttttttc agttaaatta ggacaattgc caacactaaa atggttagat ttattggctg
    11641 gcataccaac tctagttgtc actattcgat atcgaaattt attttacact gttcttactg
    11701 gtatcgttac tatggcttta ttgagattac tattttaacg tgtaggaatg gaagattaat
    11761 aaaaacacaa aaaacgcatc tatttctaga tacgtttttt gtgtttttgt ctagacagga
    11821 acttttgata gccgatgtgt aatagtttga ctaattgtta ataatatcaa cagatttttc
    11881 tgtatcttga taatatgagt tgattgtaag attgcttttt aaagatatct acatgctgct
    11941 attttaaaat ttataaaaag gtccatagtg gtttatatag tttaaaacta tataaattgc
    12001 taaaaatata aaatattaaa agctattgat attatagttt tcatggacta aaataattca
    12061 taaaactaaa agggttagtt attagctata acttgatgga ggaaattatg gtaaaagttt
    12121 caaatttagg gtatccacgt cttggtgaac agcgcgaatg gaagcaagcg atcgaagctt
    12181 tctgggcagg gaatcttgaa caaaaagatt tagaaaaaca actaaaacaa ttacgtatca
    12241 atcatttaaa gaaacaaaaa gaggcaggta ttgaccttat tccagtgggg gatttttctt
    12301 gttatgatca tgttttggat ttgtcatttc aattcaatgt aatcccaaag cgtttcgatg
    12361 agtatgagag gaatttagac ctttattttg ctattgcaag aggtgacaaa gataatgtcg
    12421 catcatctat gaaaaagtgg tttaatacca actaccacta catagtccca gaatgggagg
    12481 ttgagactaa acctcacttg cagaataatt acttacttga tctttatcta gaagctaggg
    12541 aagtagttgg tgataaagca aagccggtta tcactggtcc aataacctat gtttccttat
    12601 catcaggaat tgtcgacttt gaagcgactg ttcagcggtt attaccactt tataagcagg
    12661 tctttcaaga tctgatagat gcaggcgcca cctatattca gattgatgag ccgatatttg
    12721 taactgatga aggtgaactt ttagtagata tagctaagtc tgtctatgat ttttttgcaa
    12781 gagaagtacc acaagcccac ttcatctttc aaacctactt tgaatcagca gtctgtttag
    12841 ataaactctc taagctgcca gtaacgggat ttggccttga ttttatacat ggtagggcgg
    12901 aaaatgtagc tgctgttaag caaggtctat tccgcgaaaa agaattattt gcaggaattg
    12961 ttaatggtcg aaatatctgg gcagtaaatt tagaagaaac gttggcttta ttggaagaga
    13021 taggtccctt tgttaaacgt ttgacccttc aaccttcttc aagtctttta catgtaccgg
    13081 tgacgactaa atacgaaaca catttagacc ctgtgttaaa gaatggctta tcatttgctg
    13141 atgaaaaact aaaggaatta gaactattag cgagtgcttt tgatggtaat aaaacaaagg
    13201 gatatcacga agctttatct cgtttttcag ctcttcaagc tgctgatttt cgtcatgtag
    13261 cgttggaatc attagcagaa gtaaggcttg aacgaagtcc gtataaatta cgccaagctt
    13321 tgcaagctga aaaattacag ttaccgattt taccaacaac aactattggg tcctttcctc
    13381 aatcacctga aattaggaag aaacgccttg cttggaaaag agaaaatcta tctgactcag
    13441 attataaaga tttcataaaa actgaaatta gaagatggat tgctattcaa gaagatcttg
    13501 atcttgatgt gttagtacat ggcgaatttg agcgcgtgga tatggttgaa ttttttggtc
    13561 aaaagttagc tggttttacg acaaccaaat taggctgggt acagtcttat ggttcaaggg
    13621 cggtcaaacc acctatcatt tatggtgata tcaaacatat tcaaccctta agccttgaag
    13681 aaacggttta tgcccaaagc ttgactaaga aacctgttaa aggcatgttg acaggtccta
    13741 ttactataac gaactggtca tttgagcgag atgatatcag ccgatctgat ctttttaatc
    13801 aaattgcttt ggctataaaa gatgagattc aacttttgga acaatcaggt attgctatta
    13861 tacaagtgga tgaagcagcc cttcgagaag gcttaccctt acgccagcaa aagcaacagg
    13921 cttacttaga tgatgctgtt gcggccttta aaattgcaac ttcatctgtg aaagatgaga
    13981 cacaaattca tacacatatg tgttattcaa aatttgacga aattattgat tctatccgtt
    14041 cactagatgc agatgttatt tctattgaaa cgagtagaag tcatggggac atcattgaaa
    14101 gttttgaaac agcagtttat cctctaggaa ttggtctggg tgtttatgat attcattctc
    14161 ctcgcatacc tactaaggaa gaaattattg tcaatattca acgatcacta aaatgtctat
    14221 caaaagagca attttgggta aaccctgact gtggcttaaa aacacgtcgt gaagcagaaa
    14281 caattgctgc cttggaggtt cttgtttcag ctaccaaaga ggttcgtcag caattagata
    14341 attaataggt taatcagtaa aggattgcca tgagggagga gtaaagaaga tgtccaagtt
    14401 tttagagaaa ttaaaaacag atattctagt agctgacggt gcaatgggaa ctctccttta
    14461 tacttatggg ctagatactt gtcatgaatc ctacaatgta acacatccag aaaaggttct
    14521 agccattcat caagcttata ttgaagcggg tgcagatgtt attcagacga atacctatgg
    14581 ggctcaacgt catcgattaa agaactacgg tttggaagat caggttgtct ctatcaatca
    14641 agcagcagtt aatatagctc atcaagcgac tttgggtaaa gaaacattta ttttaggtac
    14701 tgttggaggt tttcgttctc aaagacaatg cgatttaaca ttggataata ttgtagaaga
    14761 aactttggaa caagttgagg ctttattggt gacaggacag ctagacggat tattgtttga
    14821 aacttattat gatattgagg agataacgac agttttaaaa attgtgaggg agatgactga
    14881 tctaccaatt attactaata tttctcttca tgaagcaggg gtaacatcaa atggaaagcc
    14941 tattgtagag gcgctcagcc aattagttat gctaggtgca gatgtgattg gcttaaattg
    15001 tcaccttgga ccctatcata tgatccaatc attgaagcaa gttcctttat ttgcccagtc
    15061 ttatttgtca gtttatccta atgcaggtca gttatcttta gatggtgaga atagtcaata
    15121 ccagtttagt cagaattcag agtattttgg aaaatctgca gaacttctcg tagcagaagg
    15181 agttaggctt attggtggtt gttgtgggac aactccagat catattagag ctgtgaaacg
    15241 atctattaga ggattgaaac ctattgaacg aaaggttgtt actcctataa tacctgtaaa
    15301 agattttgtt aggcgtatca gacgaacaga tactctagtg gataaggtaa aaaaagaagt
    15361 aacaattatc gcagaattag atcctccaaa acatttagat atcgttcagt ttcaaaaggc
    15421 tatacgagcg attgaccaaa aaggtattgc agcaattact ttagcagata attcactatc
    15481 taatacacgt atttgtaatt taagtatagc tagcttgctt aaagacgaaa ttagtacacc
    15541 ctttttacta catatagctt gtcgtgacca taatttaatt ggtttacagt ctagactgtt
    15601 gggcatggaa ttattaggtt ttaaccatat tttagccata acaggagatc cgactaaact
    15661 tggtgatttc ccaggagcga ctagtgttta tgatgtgact tcctttaaac tcctttctct
    15721 gattaaacaa cttaatcaag gtttatctta tagtggtgct agcttgagac gtccaacaga
    15781 ttttacagtt gctgctgcct ttaatcctaa tgtgaaaaac cttactcgaa ccgtaaaatt
    15841 gattgagaaa aaagtagctt ctggtgctga ttattttatg acacaaccaa tatttgatca
    15901 ctctgtgtta agggaattag ctgatcttac taaaacagtt gaacaaccct tttttatcgg
    15961 aattatgcca ataacaagtt ataataacgc tgtttttcta cacaatgaag ttcctggtat
    16021 taaactttct gagtcatttt tatctgctct tgaaaaggta aaggacgaca aagaagcttg
    16081 tttaactttg gccttaaacg agagtaagtc cttgattgat gaagccttga accattttaa
    16141 tggtatctat ttgattactc cctttttacg ctatgatctg actcttgagt taattgacta
    16201 tatacaaaaa aagcaagtaa gaaagtcctc ttaactttct tactttttag ttggtcgttt
    16261 attattataa taaaagttac gtaccaaaac agaggttaaa ctagattttt aagccacttt
    16321 ctatactaag ataaattagt taatttaagt tttctttaag tttatagact tagaataaag
    16381 cgttattttg atgggtagca taaaatcaag gcataatata gatgtcagcg atttttttag
    16441 actaacgaaa taacgatgta aagcttaata ggggtgtatc gaatactcac ttatatagta
    16501 ccaaaagcct atagcctgtt ggctagcttg accgtattca aatgttgaat aaatcttggt
    16561 tgagaaaggg aggtataaaa atggtatgtg tcgattttat tgatcaaaaa gtttatagtg
    16621 aagcagaagg tctatttgtt tccgatttag acttaggcat taaaggttta ataagagcac
    16681 gttatccttt cgctctggat agtgacttta tttcgtatgg gaatttaagt cattattgta
    16741 tgaattatct tgatgaaatt gttcagcgag cgaatcagaa aaatgagaag ataaaatata
    16801 atgtgactac cttgatgaaa gaagaagaga agcccttcaa cgttgaagag cgtttgaaca
    16861 agcaagcaac tatcggtcaa cgaattgctg atgatgttgc acgttttggc ggatcttgga
    16921 cttttattat cgtttttgta tccattatgg ctatttggat gttggttaat atcatgaagc
    16981 cttttggtat tcaatttgat ccttacccat ttatcctgtt aaacttagct ttatcaacga
    17041 ttgctgcgat ccaagcacca cttattatga tgagtcaaaa tcgtgctgct gattatgatc
    17101 gtttgcaagc tcgaaatgac tttaatgtca ataaaacctc tgagttagaa attagattat
    17161 tgcacgaaaa aattgaccat atggtacaac aagatcagtt tgaattgtta gagattcaaa
    17221 agttacagac agaaatgcta gtatcattgg gaaatcagtt agcacaattg aaacaactac
    17281 aaaaataatc ttttagcagt tatttaaaaa tactgataca atactaagaa tttaaattaa
    17341 tactgcaacc tcctataatg gagagcagta tttttacttt attaataaca aaaacccctt
    17401 agatgatgtg tctaagggat ttttgtatcg gaataatcga gttggtgaat tattaggcta
    17461 gtgtctgtcc gaaagcgtct gctgctaaaa tagcttgagc aaacattttg ggatcgaaag
    17521 gggtattttt gtattcatta gtagtcattg caatttcaac aatttttgga atatcttttt
    17581 ctgataaaga gacgtcagct agagttgttg gcaaacctag tgacttatta aaacgagcta
    17641 ctttttcaag ttcttctagt tcgttgaaat aagcatgtaa aactaacaca ccaaacgcaa
    17701 ctacaacgcc atgtaagaat tcaccttgtc tttgaatagc ggttgtacca tagtaaaatg
    17761 catgcgcgtg acaagaattg tagtagaaat caggttgatt aactaaatta gaagcatagc
    17821 cagttgagat taagatatca agcgcaattt cttcaactgc acgcgaagct aaattagctt
    17881 caacgtcttt tagaccttgt tcaccaaatt gataaaaagc ttcctttgaa gacagagcga
    17941 ctgcttgtcc taacactgca gtatgtggta gtttattggt cttagcctct aaggtagcac
    18001 gttctacttc aggggctttt gaaataccgt ccccaatacc agcccaaaaa tatttgcttg
    18061 gtgcctgtgc aatgatccgt gtattgataa aaatatgaag tggagactcg ggatagccgt
    18121 atcttaagaa agaatggtca tcattatata caactgcaat ggcagtacct gcggagcaat
    18181 tagaacaaat agttgggata gtaaagctat ttttacctaa ctcttttgct accattttag
    18241 ctgtgtctaa agcttttcca ccgccaaaac ctaagatagc atcagcagct tgaacttgtg
    18301 ggttggctac taacttgtca atgttagatt gtgttgaatc cgttccatag acaaagcttc
    18361 ctgtgacttt aatatttgct tcctctaaaa tagagcgaac ctcggcttct gagctgtcta
    18421 gggcacgttt gccacccacg aaagcaatgc tttgaatacg gtaagttgcc aaaacgctag
    18481 gaatttcttt gaagcaatct tctccgtagc tataatttgc cattacattt gttgtcattc
    18541 agtgtcctcc tctattccaa aggtcgatta gagacagcta ttttctaaaa gtgaatagtt
    18601 ttttgtctct tgaccaagtg attatcatat tatagcagaa tattcagaaa attggaacat
    18661 gaaaaataat ttaattttca agaataagaa tagaaaaggt aaagcgagat atacgagtaa
    18721 atactgtcag tataataatt ataagctctt gagagggtct gtattggatg caacagggag
    18781 ttcttttagg tattgatcaa gatcgcgttg gtgttttagc acctttattt tttgagggta
    18841 gtcctcaaca acttgtttat agtttgattt ttgtttgtca gagcgaccgt ctttaagaat
    18901 ccaactgata aattcaaaat ctaatttttc cgggcatcca ttggccatat caggtctagt
    18961 cttaccacga aacttacagt aacgtttgaa tgctctccac aagcagttga agcgattaaa
    19021 gtcaaaaaaa ataatctcat ctgcatctgc tagacgttct tgatatagca gttttttgta
    19081 gttaccttct ataatccatg ttcttttttc taacatgctg ctgacatcgt caatcatgtc
    19141 atcatatttt ctctcttccc aatttggggc gaagtgaata ctatcaaggt gtaatacatt
    19201 gcaattatag taatttccca attttctcgc taaagtggac ttacctgacc cactatatcc
    19261 gattattgct attttcatat actacctccg ttaaattgtt tacttgaagt taatgctgta
    19321 cttaggattt acttatttta taagggttat tcactttttt agattaatat atttttgata
    19381 taagttatcc atacacatgg taacataatt accagtaaat gtaatctaga tatcaatact
    19441 atggttgacg atattgttaa cttttgggtt caaaaaattg gggtaaaaat catcggaggg
    19501 aactgccaaa caaactcaca gtttaaaata ctaagtaata aaaagtcttg aatttattat
    19561 gtttaaggct
//
LOCUS       DATRME010000034        19027 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRME010000034 DATRME010000000
VERSION     DATRME010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..637)
                     /locus_tag="VBC23_001174"
     CDS             complement(<1..637)
                     /locus_tag="VBC23_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
                     /protein_id="HEO6843124.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDADNKSQVGQL
                     IGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTI"
     gene            complement(1326..2921)
                     /gene="cls"
                     /locus_tag="VBC23_001175"
     CDS             complement(1326..2921)
                     /gene="cls"
                     /locus_tag="VBC23_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6843125.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(3040..4710)
                     /locus_tag="VBC23_001176"
     CDS             complement(3040..4710)
                     /locus_tag="VBC23_001176"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6843126.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            complement(4799..5818)
                     /locus_tag="VBC23_001177"
     CDS             complement(4799..5818)
                     /locus_tag="VBC23_001177"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6843127.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(5845..6723)
                     /locus_tag="VBC23_001178"
     CDS             complement(5845..6723)
                     /locus_tag="VBC23_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6843128.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(6692..7510)
                     /locus_tag="VBC23_001179"
     CDS             complement(6692..7510)
                     /locus_tag="VBC23_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6843129.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(7503..7835)
                     /locus_tag="VBC23_001180"
     CDS             complement(7503..7835)
                     /locus_tag="VBC23_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6843130.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(7864..8850)
                     /locus_tag="VBC23_001181"
     CDS             complement(7864..8850)
                     /locus_tag="VBC23_001181"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6843131.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(8847..10046)
                     /locus_tag="VBC23_001182"
     CDS             complement(8847..10046)
                     /locus_tag="VBC23_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6843132.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(10039..10875)
                     /locus_tag="VBC23_001183"
     CDS             complement(10039..10875)
                     /locus_tag="VBC23_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6843133.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            11014..11712
                     /gene="coaB"
                     /locus_tag="VBC23_001184"
     CDS             11014..11712
                     /gene="coaB"
                     /locus_tag="VBC23_001184"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6843134.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            11709..12251
                     /gene="coaC"
                     /locus_tag="VBC23_001185"
     CDS             11709..12251
                     /gene="coaC"
                     /locus_tag="VBC23_001185"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6843135.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            12297..12869
                     /locus_tag="VBC23_001186"
     CDS             12297..12869
                     /locus_tag="VBC23_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6843136.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            12979..14697
                     /locus_tag="VBC23_001187"
     CDS             12979..14697
                     /locus_tag="VBC23_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6843137.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(14761..14958)
                     /locus_tag="VBC23_001188"
     CDS             complement(14761..14958)
                     /locus_tag="VBC23_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843138.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(14972..16705)
                     /locus_tag="VBC23_001189"
     CDS             complement(14972..16705)
                     /locus_tag="VBC23_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843139.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(16706..18427)
                     /locus_tag="VBC23_001190"
     CDS             complement(16706..18427)
                     /locus_tag="VBC23_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843140.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(18439..>19027)
                     /locus_tag="VBC23_001191"
     CDS             complement(18439..>19027)
                     /locus_tag="VBC23_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6843141.1"
                     /translation="KRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAENGSEA
                     NVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNISQAKKA
                     KVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTYFYYHP
                     LALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
BASE COUNT         6187 a         3667 c         2961 g         6212 t
ORIGIN      
        1 aaattgttct agaattatta gttaacatcg gtctattagc tacattagta acctgagata
       61 cttttttaga gaatgactgt ttgttagagt caccttgact agagtgatta ttttctctag
      121 atacagttgg ctttgaaaaa agtggatttt tccctataag ttgacctact tggctcttgt
      181 tatccgcatc acgttggcgg cgctctagaa cattgccctg acttttgttt tccgcatcac
      241 gttgacgacg ctctaaaaca ttgccttggc tcttattctc aacatcgcgt tgacgacgct
      301 ctaaaacatt gccttggctc ttgttttccg catcacgttg gcgacgctct agaacattac
      361 cttggctctt gttctcaaca tcgcgttgac gacgctctaa aacattgcct tggctcttgt
      421 tttccgcatc acgttggcga cgctctaaaa cattaccctg acttttgttt tccgcatcac
      481 gttggcgacg ctctaaaaca ttaccttgac tttgatttcc aacactatcc atagcagata
      541 caggacttga tcctaaaata atagttgatc ctagcactcc catataaagc caaagctttc
      601 ctgatttcca agttctaaaa cctattttat tgaacaatta attttctcct tacttttttt
      661 aaattttaaa agtcatttta ttattatttt tatttagatt atatatatta aaacatttta
      721 taatctatgc caagttaatt tgttatttaa ttacaattta ttattaattt gtatgcttaa
      781 aattaaatag ttgatatcta aaacatgata tttttaaatt ttcctttaat attataaaac
      841 atgatagata gtcatactaa cattaggatt tacaatgaga taatagtata tttttaatac
      901 ttcatctaaa ccatcagaaa ctagattatt taaaaatttt acaaagaatg tgatggtagc
      961 atattcaatt aaagttaagt aaaaacataa tttttagaaa gaaattgtga cttatttatg
     1021 caaaaaaaag aaagaaccaa actggttctt tcttttttgc ttttcagcta cttaccacag
     1081 tttgtaatga attacaagta gtagtaagct aagggcacct tttcaagtgt gataagaagg
     1141 ttcacacaac ttatcaagat ccgctcctgc gtttataatc tccctgcgca atagtggtaa
     1201 aaccacatat cgactcatcg cataacagta gtttaacata atttagttaa aatgcaacct
     1261 ttacttttta aatataacta gattcttcag tttactttta atattaaatt aaaaataaat
     1321 tatttctaaa gcaggggagc aatagttttt gtcactatgc caattacttt ttggaatttt
     1381 ggaatattat ttatatcttt caagctaact aaccttgatt tatttaacgt atccatataa
     1441 tctcgataaa tgtcttggat tgcatctacc ttataaagat aaacagcaca ttcaaaatgg
     1501 tgatacaggc tacggtaatc aagatttatc gtacctacaa cagcctttgt attatcacta
     1561 aggaatattt ttgaatgaac aaatccaggg gtgtattcat atattttaac acctgatttt
     1621 attaatgctt gatagtaagt tttagcaaga gcataaggga ttggcttatc tgggataccc
     1681 ggcataataa tacgtacatc tacaccacgt tccgcagcaa actgaatggc atgttccaat
     1741 tcactgtcta atattaaata aggtgtcatg atgtaaacat attctctagc atgattaaga
     1801 atatctatat aaacattttc ccctacttta tcagtgtcta aaggagaatc tccataagga
     1861 ataacatagc cttcattttc aactaaatca tcgtgtacac cgaggtaagg cgctactaac
     1921 attttttcct cagtaatgga ccacatctgt aagaaaagta ggaggaaact atccactgct
     1981 ttccccttta acataatacc tgaatctttc caatgcccaa ataactcaat gtgattaata
     2041 tactcatcgg ctaagtttac accaccagtc atgcctacta ctccatcaat aacaacaatt
     2101 tttcgatggt cacgatagtt ataataagtt gaaataaaag gagaaatcgg agaaaatgct
     2161 tttgctttta ttcctatttt ctcaagtcgt ttcgtataat caaaggataa ttttgtaatt
     2221 tctatcatac catcatataa gatacggact tcaacacctt cttccacctt ttgttctaaa
     2281 atactaagta tttctcccca cattattcct tcgtcaatga taaagaattc taaaaagata
     2341 tacttcttag cttttagtag ttcttctttc attttttcaa agaatgcttc tcccgttggt
     2401 agaaatgtca catcagtatt tcgataaact gggaaatttc catgtgcttt ttcaaaatat
     2461 tgaaccaaat gatatgttgt ggatgtactc gacttaagct gattcaaagt ttcaggatca
     2521 tcctctagat aaatctgact ctcatcaatc aatgtctcca atctttgttt caaaccacga
     2581 taaccccagt cgaatttggt gtacattagg aacatcgccc ctagtaaagg ggcaatcatt
     2641 accaatatta accaagttac tcgggacaaa gcatccattt cactattaac taaatataag
     2701 accgctccaa ttgttaagat atgctcaacg gttgctaacc aaacacgata ctgctctagc
     2761 catgagtaag aggctagtaa aaacaataat tgtaaaatca ataaaatagc gataacggtt
     2821 gttcgactaa atatacccct tagtaaactt ttcttactct tattaagtaa acgaacaatt
     2881 ttgactttat tactgataag tttttccccc ctccaaatca atcattttat tatagcatac
     2941 tgtcacttga atagcattct attattctaa attatctcta gttaaaatct aatccccaat
     3001 aaaaagaggc tagcatcaca agccctacct ctttattatt taaaataatc caacggctgt
     3061 accgtcttct aaaacatcca tattgagagc tgcaggtttt ttaggtaaac caggcattgt
     3121 caatacgtct ccagtaagag caactataaa accagctcct gtttttggaa caaattcacg
     3181 aacagttata tcaaagtcag ttggagcacc aaataaattt ggattatcag aaaagctata
     3241 ttgtgtcttt gccatacaaa taggcatctt gtcccagcca ttgtcactaa actcttttaa
     3301 ttgtatttgt gccttaggtc caaaatgaac tttattacca ccatatattt tagtaacaat
     3361 ttttttaatt ttttcttcaa tagtgtcttc atcattatat aagcgtttgt aattggatga
     3421 ttgtgtttca atcacattag caacagtctg tgcaagttca aggccaccgt ctgctccatc
     3481 ttcccacaca cttgctaatt caactgccac atcaatatta ctacataagg ttttaagagt
     3541 tgcaatttca ctttctgtat ctgcagtaaa ctgattgata gctacaacta ccggaacacc
     3601 atattgacgc atattgttaa cgtgacgctc gagatttgta aaacctcttt taactgcttc
     3661 gacgttctct tgtgagagat cttcttttga aacacctcca tgcattttca atgcacgcaa
     3721 tgtagcaaca ataacaatcg catcgggaga agtcggaagg tttggcgttt ttatatcaag
     3781 aaacttttca gcgcccaagt ctgctccaaa accagcttca gtaacaacgt aatctgctaa
     3841 gcgtagagct gttgaagtcg ctaagacaga attacatcca tgtgcaatat tagcaaatgg
     3901 tcccccatgg acaagagccg gtgtcccata aatggtttga accaaattag gtttgatagc
     3961 atcttttagg ataagtgtga gagctccttc aatcttcaaa tctttaacat agattggttt
     4021 acggtccctt gagtaagcca caacaatatt tgacaggcgt ttctttaaat ctgataggtc
     4081 agtagctaaa catagaatag ccattatttc agaagcaaca gtaatatcaa agccatcttc
     4141 acgtggtatc ccattgacag ggcttcctaa gccaacgatc acctgacgaa gagcacgatc
     4201 attaagatca actacacgct tccaaatcac tcgacgttgg tcaatatcta attcattgcc
     4261 ttgatgaata tgattatcaa gtagagctga taaagcatta ttagcagttg tgattgcgtg
     4321 catatcacca gtaaaatgca aattaatatc ttccattggt agtacttgtg cgtatccacc
     4381 tccagcagct cctcctttaa tacccattac aggacctaaa gaaggttcgc gaagcgcaat
     4441 catcgttttt ttaccaattt tatttaaagc atcagctaat ccaatgctca tagtggattt
     4501 tccctcacct gctggtgtcg gattaatagc agttactaaa attaactttc ctactttttg
     4561 agatcttaca gcttcaatct tatcaaaaga taattttgct ttgtatttac catacaattc
     4621 tatatcatca aaaccaattc ctacttgctc aacaatctca gcaataggct ttagggcgac
     4681 gctttgcgct atttcaatat ctgttttcat gatattctcc ttctaaataa taaaaatagt
     4741 atagcaatat tccctataaa aacatatttt tttgcaaaaa aaatttaaaa tgttcgtctt
     4801 atgaaaataa aactgctttt atttcttctg gactagtctg accaaaataa tcttctaatg
     4861 ataaagtact taatttttcc aagactaatt cttctttcat agggcaacca atgagaaggt
     4921 tttcaatatc tgaaatatct ttagaactga aaaagtcacc ataaatacgg caattcgtaa
     4981 taataccttt ctcaatttct aagtgaaaat cgtaagttcc tgacggaaaa cgagcatctc
     5041 tgtgatacga atactgtggt gagttaccat aattccaatc ccaattctta tatttttcat
     5101 cagctagagc gtcaatcgca gcccaatctt tttcggttag atgatagcgt tttgcctgat
     5161 caatatgatc aatatgtaat aattgacaaa ccattaaatc tttaaattga tcggttgtga
     5221 tatgacgata atcttctgac aagtggctac gaatatctcc tactcgactt cggacagatt
     5281 ttatcccttt agattcaatt tttttccgat taggatttaa cactttttcc atagcatcaa
     5341 aatcaacatc taataaaaga gagtaaccac cgtaaatacg accgtttact atcgtcattg
     5401 ctgcacctga tactttttta ccatcaataa ctaagtcatt tcgtcccctc atttcaacac
     5461 tagaagcacc taaagttttt agggccttaa tagctggctc ataagctcgt ttgaaatcac
     5521 caaattgtcc atgatctttc attagatagc aaatatttac agcacctgag tcaacgtaaa
     5581 cagctcctcc accagtatct cgacgaacaa caggaatatc atgttctttc aaatattctt
     5641 gatttgtctc tataactgca ttctgaaatt ttccgatttc aactttagga tcacaatagt
     5701 atggaaataa aatatcatca tctaaaaaaa cattttgtaa aatataaact tgcatggcaa
     5761 gtgcaatcgc accgtctgtg atacgcttac catttctaat tggttcaata agatacattt
     5821 aggtctcctt ttatagcaat ttagttatcc aataatttct tttttcgctt cagcaattaa
     5881 ttcagcgata tgctgtgtca gttgcacact ccttgagcga atcttttgag gaatgcgata
     5941 atgcttgtga ttaagagtaa caaataaaga gttttcattt aaagagacga atcgccaaaa
     6001 cggatgctta ataaattgtg gcgtggtatg tccaactcca atctctagat aaagaacttt
     6061 gtcattttga tgttgtgata agaaattttc ataacgttgt cgttgagcat gaaagtcagc
     6121 atcttccacc ataccttttt cttcgttacg tttattaatt tcaaacggtt gatcacactt
     6181 tgggcatttg gggattaagt tactaggaat cttcatatcc ttttgttgtg caatcatttg
     6241 acgaattgcc tgatcatttc gataagtttg ttgatgacaa tgttgactac actgccataa
     6301 gccatattca ccttgaatat gaaaaacttt ttctaaatta taatcagcca cagcgaaggc
     6361 attatcagca ttagttgtaa taatatgata ttcttttttc gctaaaatat cctttaaatc
     6421 aagataagct tgtcctaccg gttgatctaa ataattaagt gccacaaaac gactttggaa
     6481 ggcccaatat tcttcccaat cttcaaagtc ataaagactt gcttgcaaca tatctaatag
     6541 ttgatattta gcaataaaat ccggaaaagc ttcttcaaat ctggggccaa tataggtaaa
     6601 tccatccgct gcagacatgc ctgcaccaat accaactaca atagcatctg attcttctat
     6661 taactgactt agtatttctg attgagaatg attagttttt tctagagtct tccacactgt
     6721 catctatttc ctcttttaat aataattttt gataaatctc ttggtcttta ggtgtgaatg
     6781 tattgaaaat aatatagagt ttgtgctgat tttcgcgttg ccactctaat atctcttgaa
     6841 cagccaattc agcggctctt tgattaggaa aaccaaattc tcccgttgat atacaaggga
     6901 agacaattgt tttaagatta gcttgagctg ccaattgtaa gcaggattta taagaagact
     6961 ttaataaatc ctctcttata cgagatggct tttgatcaac ttttacataa ggtccgactg
     7021 tatgaataac atacttacaa ggaagatgat aggattcggt aagtttagct tgtcctacgg
     7081 cttccattcg tccttgttgt gtcatcaatt gatgacatgc taaacgtaat cgtgaacctg
     7141 caaacgtatg aatctgattg tcaatacaat ggtgattagg aataaaacaa cctaacaact
     7201 tactatttgc tgcattaaca atagcatcca cttgtaataa gcaaatatcc ccatggtagg
     7261 taaatacatt tgaatatacc gtttgatgac agacgtctat cgtttttact ttttgagtat
     7321 tccaccaatg tgacaaatac ctatcttcta aagacaggta ctcttgacta atgttttgag
     7381 gatcttgttg attaactaga ccacgccaaa ccgtttgcaa atcggtggtc ctcaagtcat
     7441 caacgtctgt taatttttct gactgaagat actcaatcat ggctagcaat agttgttttt
     7501 gattaggcat tttctaaagc ttcaaaagca gcataatcaa catctgttaa cacaaccaac
     7561 caattttcgt cagctttttc agaatttaac aaagttggtt cttgacttgc agctgtatta
     7621 actttaacaa ctttacctgt aagaggagat aaaattgcca taaccgtctt tgaagcttca
     7681 atatttaaaa taacatcatc aacttctaaa tttgtatcat ctgtgaattc cacatatcca
     7741 acagttccca agtcatcttg aagttcggga gttaagctga tagtataaag ttcttcattt
     7801 ttttcaatta ataagtagtt cgcaattttt ttcatgagta atttcctttc tgagaaaacg
     7861 tgattaatat aactgtgata ataattttaa ggatttgatt cgttgatcaa gtcctggaac
     7921 aagaggaatc aaaagtagtt cttctgcttg actagcatta actaaagcgt caaggctttc
     7981 tttaacttgt tttggatcac ctacaatcat acggtggcga tttgatttaa tttttgcttt
     8041 ttgttcagga gtcaattggt aatgattagc ttcttcaata gtaggaaatg ttgcaaattc
     8101 attgaagtct ttatttccta gcatccagat atctaaagtc tttgctatgt tttcagcttc
     8161 ttctgaagta tcagctataa ctacaaatgg agctaccatc aaattaggag acttattagg
     8221 ataatattct tcaaactgat gataataaag agatgaaagc tttttagctt ctgtcaatgg
     8281 gtctttgtcc ataaaaggaa aaacaccgaa ggtaaagcct aagccaagtt tagccgctaa
     8341 ataagctgat ttttgaccac tccccaacac atataagtct gggaagctag aaagagtcgg
     8401 ttgaactaat ggttccttac tggatgattc atcaataagc catgacttca attcctccag
     8461 tacctcttcg taatcatgtg ctttatgtaa gctacgaagt gcatttgaaa ctttaactgt
     8521 ccctagtgaa tttcctaaac caatacttac tcgattcgga tgacatgtct ctaatgtctt
     8581 gagagtctcc gcgagtttaa aactactata gtgcagaggc attatacctc cagagccaat
     8641 tttgatagat ttagtctggt tagccaaatg cattatcatt aattctggat tgctaatact
     8701 gaatgcctta acaccgtgat gttcagccac ccaaaattga tgatagccta gttgctccgc
     8761 ctcttgtgat aaaagaatcg tttcttgtat tgcatcactt gcagtttttc cataatcaat
     8821 aagcccataa tcaaggacac ttaatttcat ctttcctccc tataattgct tcgaagttct
     8881 ctaaaaactt ctcgagcttc ctttggtaaa gattctccat aatccatcaa aggaacaata
     8941 tctttaaagg aggcttttgg aatcgccaaa ccttctaaat cattcacttt aatccttaaa
     9001 ttaatctgat cagaacgctt agcttttatt ttttgtaaaa attccggatc aactactaag
     9061 ggcgtggaag ctcctatcat atgagcatgt tgtaaggctt caaacacctt tgaagcacta
     9121 ttaatacctc cagttgccat aattggaact ctatcaccaa aatgttcaaa tataacttga
     9181 ttaaccagct gccctttgta aactccctca gaacgtatag tatttctaaa gacatcatgc
     9241 ccccagcttg caatggcaag ataatctacc tgagcaatcg ctaaaatttt ttcaagaaat
     9301 tccataaact cttcaatgct atatccaatt tgtgaaccgc gagtttcttc aggcgtcgct
     9361 ctaaaaccaa ggataaaatc tgactccgct tcttctctga taactttttg taccgcttta
     9421 aaaacttcta agcctaaacg acagcgatta gtcaaagttt gaggaccata ctcatctttt
     9481 ctttgatttg agaaggttga gaaaaaagtt tgaattagta atcgttgtgc agaagatatt
     9541 tcaacaccat caaatccagc ttgtatagct cgtcttgtag cctggacata ttcatcgata
     9601 atccttagta tatctttgtg tgtcagttct ttaacctgat gtgggtatgg cgattgcaat
     9661 tgcatagggc tgggaccata tacatatccg tgtcttgcca aagtgtgact tgagaaacgt
     9721 ccagcatgcg tcagctgcaa aacagctttc gctcccttag acttcattgc tttagccaat
     9781 ttagtcaaac caggaatgtc ctcatccttt gaaacgctaa aaccatattc aaataactga
     9841 ccatattctg tgatatatgc tgctccagta atttgtaatg gagcggattt agcacgacga
     9901 acagcatatg ctatgtcgtc atcagttaca aaaccttctg atgtagaaga attagttacc
     9961 ataggtgata gaacaaaacg attttctaag ctcaatccat tgggaagtgt taatggacga
    10021 aataaattac cttggacatt attcatctct gctcaccatc cccttattag ccatagactt
    10081 agcctgaaat ctatcaaact ctgcaactaa atcgggtcta atcatcagta aacgatcatt
    10141 aaaacctact tgtttaaggc taatcaacat acgatcaata acatcttcta ctgtcatagg
    10201 acaatctaat agatcagata gattagccat aatttcagga tctacatttg gataagcaat
    10261 tggactaccc gtatcaccta gaccaatctt ataaaaatct gaaatcattt gactccgccc
    10321 tttttgatcg ccacaaacgc taaggtaaat tgataccgca ataccattct ttatacggcg
    10381 ctgagccaag ccagcaaatt ttttgccatt tatactaaga tcaaacttac caggacaata
    10441 ggaggtctct acttcaaagt gctcaatagg ttgataaaaa tccgaaaata tacttctaat
    10501 aaaatcgacc attataagat acccatctga gatagacaat tttctctcaa aaacatctgg
    10561 aataaccaat gaaaaattta aaattccttc atcagctaca actgcgagac ctccaaaatt
    10621 ccgaacaact ggttcataac cacgactgat gatttctttt ttagctaatt ctaaatgtgg
    10681 taattctcga tctaacatcc ccagaatgac tgtcttagtc atcggccaaa tatgcaaaat
    10741 aatttcttga tttgagcgat taatctccct taaaaatact tctgtccata taaaaggttt
    10801 ttccgcgtct tgagcatctg taacatagtc tttaaaaata gatacaggta actgcgctag
    10861 atcttgccac tccaataaat ctgacctcct ttctctagaa aactctgcaa gatttatttt
    10921 atcgcaatcg cttgaaattt tccagtaagt ttccctcaaa ttccattact atcaagaaga
    10981 aatatatctt ttatttacga aaagcatttt tttatgtaca atatggctat gaaaattttg
    11041 attacatcag gtggaactac cgagaaaatt gataccgtta ggagtatcac gaatcacgca
    11101 acaggagctt taggaaaaat aattgcagaa aaatatttac gagaaggaca tcaggtgaca
    11161 ctcgtgacta ctaaaaatgc agtcaaacca gaatctgcta ccaacttatc catatttgag
    11221 attgaggacg ttgactcatt aataaaaacc ttgaagccat tagtgaaaga gcatgatatt
    11281 ttaattcata gcatggcagt gtctgactat acccccgtct acatggctga tttcgaaaag
    11341 gttaaatcaa gcgatgattt agatactttt ttgcgcaaag ataaccacga agggaaaata
    11401 tcatctgaat cagaatatca agtccttttt ctcaaaaaaa caccaaaagt tatcagcctt
    11461 gtgaaaaaat ggaatcctca aattactcta gtaggtttta aactacttgt gaacgttact
    11521 aaagaaaacc tttttaaagt tgctcgtcat agcttaatca aaaataaagc tacgtttatt
    11581 ttagctaatg acctcattga tattacctca aaacatcaca tagcctacct acttgatcat
    11641 gataatgtgt ataaagctac caccaaagaa gatattgctc aattaattta cgagaaggta
    11701 aaaaaaatat gattaaacgt attacactag ccgtcacagg ttcaatttcg gcctataaag
    11761 cagcagacct tacaagtcaa ttaactaaaa ttggctatga tgttcatatc ataatgacac
    11821 aagctgccac acaatttatt acaccgttaa ctctgcaagt attatcaaaa aatcctattc
    11881 atttagatgt tatggatgag cataacccaa aaatcatcaa ccatatcgaa ttagcaaaac
    11941 gcacagattt atttatcgtt gcacctgcct ctgctaatac tattgctcat ctagcatatg
    12001 gcttcgctga taacatcgta acaagcgttg ctcttgctat gcccgatgaa actccaaaac
    12061 ttatcgcacc agcaatgaat acaaagatgt atcataatac aatcacccaa cgtaacattg
    12121 atattttgaa aaaaataggt tatcaggaaa ttgaacctcg tattagtttg cttgcctgtg
    12181 gtgataccgg tcaaggagcc ctagctgata tctctactat acttaaatgt atacaagaag
    12241 ttgcaaatta ggctgtaaat ttgtatacta tttctagttt aatttaagga gttataatga
    12301 aaaagaaaaa caaatcctct aacattgcta taattgcaat cttttttgct attatgcttg
    12361 tcattcattt tttgtcatca tttattttta gtttttggtt agtccctatt aaacctactt
    12421 tgatgcatat cccagttatt attgcatcta tagcctatgg acctcgtatt ggtgcaactc
    12481 taggcgcctt aatggggggg atcagcgtag ctaacagcag cattgttcta ttaccaacga
    12541 gttacctctt ctcacctttt gttgaaaatg gtaattttta ttcgctaatt attgcacttg
    12601 taccacgtat tctaatcggg attattcctt atttcgttta caaattacta cacaaccgct
    12661 ttggtttggc tatctcaggt gctataggct ctctaacaaa cacagtattt gttttatctg
    12721 gaatttttat ctttttttca agtacttata atgggaatat caagctaatg ctcgctggga
    12781 ttatttcatc taattcatta gctgagatgg tcattgcagc tatcattgta tatctaactg
    12841 ttcctcgtat tctcaatatt aaacattaaa aactggccag gaagaaatct tcctggcttt
    12901 tcttacttat attttatagt catattgaaa aaaacatgtt ataatgaaag cgtttataaa
    12961 tattgaagga gtatttcaat gacttacaca gaaaattttc aaaaatggtt agattttgag
    13021 cagcttcctg actatcttcg tcaagaatta ctatcaatgg atgaaaaaac aaaagaagat
    13081 gctttttaca caaacttaga atttggtact gctggtatgc gtggttatat tggtgctggt
    13141 acaaatcgta ttaatattta tgttgtccgt caagcaaccg aaggattggc aaaattaatc
    13201 gaaactaagg gagaggaagc taaaaaacgt ggtgtagcca ttgcttatga ttcacgccac
    13261 ttctctccag aatttgcttt tgagtctgca caggtcttgg cacagcatgg aattaaatct
    13321 tacgtatttg aagctcttcg tccaacacca gaactctctt ttgctgttcg ccatttaaat
    13381 gcctatgctg gtattatggt tactgcaagc cacaatccag cacctttcaa tggttataaa
    13441 gtctatggac aagacggagg tcaactacca ccagctgatg ctgatgcact aacagacttt
    13501 atccgtgcta ttgaaaatcc atttgcagtt gaacttgctg atttagatga aagcaaatca
    13561 tcgggcctta ttcaggttat cggtgaagat gttgatattg aatatcttag agaggttaaa
    13621 gatgttaata tcaatcaaga tttaatcaat aactttggaa aagacatgaa aattgtctat
    13681 actcctctcc acggaactgg tgagatgttg acacgccgtg cccttgctca agccggtttt
    13741 gaatctgttg ttgtagtaga atcacaagct aaggcagatc cagacttttc tactgttaaa
    13801 tcgcctaacc ctgaaagcca agctgccttt gctcttgctg aagaactcgg acgagaagtt
    13861 aatgcagatg tcttagtagc tactgatcct gatgcagacc gcctaggcgt tgaaattcgc
    13921 caaccagatg gttcttataa aaacctttct ggaaatcaaa ttggagcgat tattgcgaaa
    13981 tacatcctgg aagctcataa aacagcagga acacttccag aaaatgctgc tctcgctaaa
    14041 tcaatcgtat caacagaatt agtaacaaaa attgccgaaa gttatggcgc tacaatgttt
    14101 aacgttttaa ctggattcaa attcatcgct gagaaaattc aagagtttga agaaaaacat
    14161 aaccacacct atatgtttgg ttttgaagag agcttcggat atcttattaa accatttgtc
    14221 cgtgataaag acgctattca agcagttctt ttggttgctg agattgcagc ttattaccgt
    14281 tcgcgtggtt taacacttgc agatggtatt gatgaaattt acaaagaata tggttatttt
    14341 gctgaaaaaa cgatttcagt aacactttca ggagtggatg gagctgctga aattaagaag
    14401 attatggaca aattccgtga aaatggtcca aaacaattta acaatactga tattgtatta
    14461 ttagaagatt tccaaaaaca aatcgctact aaaaatgatg gaacaatatc taacttaaca
    14521 actcctccaa gtaatgtttt gaagtacacg ttagctgatg attcatggat tgcagttcgc
    14581 ccttcaggaa ctgaaccaaa aatcaagttc tatattgcta cggtcggtaa tgatttagca
    14641 gatgcagaaa caaaaatcgc caatattgaa aaagagatta caacatttgt aaattaataa
    14701 aaaaagctag gaacttactc ctagcttttt ttatatctat ctaacgatgt tctattattt
    14761 tcaatcatga aatataactg caattaactt cacgatcaaa taatagagca gacttacaat
    14821 taaccacgtt atcccaataa ctgcaactaa acctattaaa acagataata ccgtattctt
    14881 agttgctagt tggttactga taaaccatga aactgcagaa ataaatatca gtaataatag
    14941 ttgaagcctt tttttcatat aattcctctt actacttttg tgcattttgc atttgataat
    15001 aaataccttt ttgagctatt aattcagagt ggttacccac ttctacaatt tcaccagata
    15061 ccataactaa aataatatct gctgtttgaa tggtagataa gcgatgtgca ataataaagc
    15121 ttgttctccc cttcatcaac atttggaatg cttcttgaac aagaacttca gtacgagtat
    15181 ctatagacga tgtcgcctca tctaatatca atattctagg taatttaaga aaaatacgag
    15241 caattgtcaa tagctgacat tgcccctgtg ataacgaatc tccagcatct tctaaatacg
    15301 tatcatatcc attcggtaat tgtttgataa agaaatctgc attagctgct tttgcagcct
    15361 ctatcacttc ttcacggcta gctttaggat tggcatatgc aatgttatcg tgaatcgtag
    15421 cactttttaa ccacgtttct tgtaatacca ttccaatttc ttgccttaat tgtgatggct
    15481 cataatcagt aataggctta ccatctaaaa ggatatttcc cccgtcaact tcatagaaac
    15541 gcattaataa attgattaaa gtagacttcc cagcacctgt tggtcctaca atagcgacct
    15601 tagctcctgc tggaatgtgt aaattaatac cattcaatag tggttttgat ttattataac
    15661 caaaaataac gtttttaaaa tcgatttgac ctttgaccgt tgatgaatca agtttttctg
    15721 tacctgttat atttggtgac gattcttcta aaatagagta aaggcgttct gcacaagcca
    15781 gagcactttg catctcagac aggacggaag agatgtcatt aaatggctta gtatattgat
    15841 tgacataatt taagaaagtg attaattgac caacactaaa agcacctgac ataatacgaa
    15901 gggcaccaat ccctgctaag aagccataaa tcaaactatt gataaatcgg gttgatggat
    15961 tgacagttga agaataaaaa attgctgatt gagaaaagtt agcatactct tgattaatag
    16021 tcctaaaatg atcactactt tcttcttgcg cactgaatgc ttgaataagg ctttcttgac
    16081 tcaccatctc ttcaatgaat tgagtttgtc gtcctcgtga tgccgtttga ttttgatata
    16141 agtgataact ctttttagca ataaagcggg ctagaaagag tgataaaggc gtcagaaata
    16201 ataccaaaaa aagcattaat aaatcaatct ttgccatact aaaaatagta actagaatag
    16261 ttaataaacc aacaaagaac tggttaaaaa ccattaataa accatttgat aattgctccg
    16321 tatcggtagt cacacgacta atcaagtcac ctatgccacg tttatcaagg taagctatag
    16381 gtaatagatt caacttttcc attacagcct ttcttaaaga agcaacataa tgaaaaatta
    16441 agcggttata gagcagagga ttaatccatt gaataatagt atttgctaaa ataactgcaa
    16501 ccatcttcca cattataggt aacaataata tcatagaatg aggcgataat acaacgtcta
    16561 ccgcctgtcc aattagaaca ggcaaataaa cagtgaggca aacttgtact accgtcccta
    16621 ataatgccat tcctactaag ctttttttag aaagaagatc ttgaacaagc tttttttgta
    16681 tctttttagg atgacttgct ttcatttact cctcctcctt aaaattttgt gaatggtaaa
    16741 tggacttata aactttgtta tgagctaata aagattgatg attatcaaaa ccaacttgtt
    16801 tgcccttatc aaggagtaag attctatctg cattttgtat actattaatt ctctgtgata
    16861 caataataag attagtttgg ttgaaatgtt taattatagc tttaaataaa cgagcttctg
    16921 ttaaataatc caatgcagat gttgcatcgt ctaaaatcaa aaatggaatc ttatcttgaa
    16981 ctaaagctct tgcaatcgtc aacctttgcc tttgaccacc agagaaattc ctaccaaaac
    17041 tctctacagg ggcatcaagt tgaccatctt tttcttttac aaaatcacta gcttgtgcta
    17101 tttctaaagc agtccaaagt ttttcctcac taactttacc aagtcccaaa gaaaggttag
    17161 aacgaatagt tcctttaaac aactgagcat tttgaggaac aactcttacc aggctacgcc
    17221 aatttgaaat tgtatctgga ctttttccct gatgatatat atcaatatcc ccttcttgca
    17281 ctttataaat atgaagcagt aaattaacca aggttgattt cccagagcca gttccaccaa
    17341 ttatccctaa agtttctcct acattcatag aaaaagtaat atcagataaa gctttttcag
    17401 aggagtttgg ataagaaaat gccatttctt gcacttctaa agccttattc gaatattttg
    17461 gctcaagttt gtcatcaatt atttcagacg gtctttcaaa aacggctata attcgcttag
    17521 cactgatata actttggttc agtgatgtca ccaacattgt catttttaac aattcaacaa
    17581 gaatttgcaa taagtaatta attaaagcaa ccaacattcc ttgtgataaa agatgattag
    17641 ctatatttaa attaccacgc caaataatca caaccaaagt gatattaaca actagaaatg
    17701 ttagaggcgt tactaaacta gaaagcctac ctgcttttaa ttgtaaattc gtatagtgat
    17761 aattgatatc attaaatgct tcactctctc tatctacttg attaaaggca cggataactc
    17821 gtacaccttg aagttgttgc ctagtcaact ttaccaaata atcggttgaa gttctgattt
    17881 taaggtaaat aggatttaat agtcgagaca ttacaaaaac aatgataaat aacgtgacaa
    17941 ccattatcaa gaaccaaata gtcaatgagg gactaatgga aaaagccata attatggcac
    18001 caaaaacaat aatcggagcc ctcaaaaata aacgtaaaaa ttgatttaaa ccagtttgga
    18061 tgtgaaatgt atcagcagtc aaacgagtaa tcaaactagc agttcccaat tcatctctgt
    18121 cttttttacc cagtgacatg actttttgat agagatcctc tgtcaattgt ctcgtataac
    18181 caactgcggc ttttgaagaa taatattgag cagttatcgc tactacaaca cccaccgcag
    18241 ccaaaaagaa aatcaatcca atttgcaaca gtaaactact tctatcccct cgtggaatgg
    18301 tctcgtcaat catttttgca ataacgattg gtaccaaaag ttcaaaagag gcttctaata
    18361 atttgaaaag gggtccaaag atagtttctt tgatataccc cttaaaataa taagttagtc
    18421 ttttcatact tacccttctt aaaacaactc cattattttt atcaaataaa ggttgaattg
    18481 aacttcacgt gagtagtaaa tattaccacc atttttatag agcttccctc ctgaaattaa
    18541 ggcgagagga tgatagtaaa agtaagtgtc cccgttataa tttcctaggc tgggagctac
    18601 aacattttta gagtaatttt tggccaactc aatggaattt tcacccccat gatccgctac
    18661 ataatcaata taagaagaac caaaattata tgcttggaca gcagtccata catcaacctt
    18721 agctttttta gcttgactta tattttgtga caacagagtg acgccatgtt taatactggt
    18781 gtgactatcc gtaatactgt tcgtagttcc actgatactt tcacttgatt gcatcacatc
    18841 tatagcatct cccttagttt cagtataaat catagataat actaagtgca cattagcctc
    18901 tgaaccattc tctgcaagag tattctgcac taaatcatga tattgaagca cttttttaac
    18961 attttcatgg atgataacta gacggtaccc aatgaaaata ataataatta atgctatcaa
    19021 acgtttt
//
LOCUS       DATRME010000035        18257 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRME010000035 DATRME010000000
VERSION     DATRME010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18257
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            146..571
                     /gene="rplK"
                     /locus_tag="VBC23_001192"
     CDS             146..571
                     /gene="rplK"
                     /locus_tag="VBC23_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6843142.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            675..1364
                     /gene="rplA"
                     /locus_tag="VBC23_001193"
     CDS             675..1364
                     /gene="rplA"
                     /locus_tag="VBC23_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6843143.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1753..3369
                     /gene="nikA"
                     /locus_tag="VBC23_001194"
     CDS             1753..3369
                     /gene="nikA"
                     /locus_tag="VBC23_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO6843144.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            3356..4300
                     /locus_tag="VBC23_001195"
     CDS             3356..4300
                     /locus_tag="VBC23_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843145.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4300..5106
                     /locus_tag="VBC23_001196"
     CDS             4300..5106
                     /locus_tag="VBC23_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843146.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5094..5882
                     /locus_tag="VBC23_001197"
     CDS             5094..5882
                     /locus_tag="VBC23_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843147.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5869..6549
                     /locus_tag="VBC23_001198"
     CDS             5869..6549
                     /locus_tag="VBC23_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843148.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            6670..7398
                     /locus_tag="VBC23_001199"
     CDS             6670..7398
                     /locus_tag="VBC23_001199"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6843149.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            7414..7971
                     /gene="frr"
                     /locus_tag="VBC23_001200"
     CDS             7414..7971
                     /gene="frr"
                     /locus_tag="VBC23_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6843150.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            8089..8943
                     /gene="cvfB"
                     /locus_tag="VBC23_001201"
     CDS             8089..8943
                     /gene="cvfB"
                     /locus_tag="VBC23_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6843151.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            9081..9590
                     /gene="msrA"
                     /locus_tag="VBC23_001202"
     CDS             9081..9590
                     /gene="msrA"
                     /locus_tag="VBC23_001202"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6843152.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            9587..9802
                     /locus_tag="VBC23_001203"
     CDS             9587..9802
                     /locus_tag="VBC23_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6843153.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            9959..11731
                     /locus_tag="VBC23_001204"
     CDS             9959..11731
                     /locus_tag="VBC23_001204"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6843154.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            11855..12841
                     /locus_tag="VBC23_001205"
     CDS             11855..12841
                     /locus_tag="VBC23_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6843155.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(12932..13735)
                     /locus_tag="VBC23_001206"
     CDS             complement(12932..13735)
                     /locus_tag="VBC23_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6843156.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            13927..14403
                     /locus_tag="VBC23_001207"
     CDS             13927..14403
                     /locus_tag="VBC23_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6843157.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGRMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            14725..15531
                     /locus_tag="VBC23_001208"
     CDS             14725..15531
                     /locus_tag="VBC23_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843158.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            15926..16423
                     /gene="ybeY"
                     /locus_tag="VBC23_001209"
     CDS             15926..16423
                     /gene="ybeY"
                     /locus_tag="VBC23_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6843159.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            16404..16802
                     /locus_tag="VBC23_001210"
     CDS             16404..16802
                     /locus_tag="VBC23_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6843160.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            16844..17743
                     /gene="era"
                     /locus_tag="VBC23_001211"
     CDS             16844..17743
                     /gene="era"
                     /locus_tag="VBC23_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6843161.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            17980..>18257
                     /locus_tag="VBC23_001212"
     CDS             17980..>18257
                     /locus_tag="VBC23_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843162.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFIIMKGCLTS"
BASE COUNT         6082 a         2686 c         3620 g         5869 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttca
      181 aattcctgct ggtaaagcaa ctccagctcc accagttgga ccagcacttg gtcaagcagg
      241 aatcaacatc atgggattca ctaaagagtt taacgctcgt acagctgatc aagctggtat
      301 gattattcca gttgttatct cagtatatga agacaaatca tttgatttca tcactaaaac
      361 accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtgaacc
      421 taacaaaact aaagttgcaa caattactcg tgcgcaagta caagaaattg ctgaaactaa
      481 aatgccagat ttaaacgctg caaaccttga gtctgcaatg cgtatgatcg aaggtactgc
      541 tcgttctatg ggattcactg ttactgacta atcagaaaca cccacataac ccgcaagact
      601 tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca aatcgattga ccacattaca
      661 aggagaattt taaaatggct aaaaaaagta aaaacttacg tgctgctctt gagaaaattg
      721 acagcactaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
      781 ctaaatttga tgcaactgtt gaagtttctt ataaccttaa cattgacgtt aaaaaagctg
      841 accaacaaat ccgtggtgct atggttttac cagcaggtac tggtaaaact tcacgtgttc
      901 ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc agcaggtgca gactttgtcg
      961 gtgaagatga cttagttgct aaaatccaag gtggatggct tgactttgat gttgttatcg
     1021 caacaccaga tatgatggca cttgttggac gtcttggacg tgtccttgga cctcgtaact
     1081 tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt tgctaaagca gttgaagagt
     1141 ctaaaggtgg taaaattact taccgtgctg ataaagcagg taacgtccaa gcccttattg
     1201 gtaaagtttc atttgatgat gctaaattag ttgataactt caaagcattt aatgatgtta
     1261 ttgttaaagc taaaccagct actgcaaaag gaacttacat cacaaacctt tcaattacaa
     1321 caacacaagg tgttggtatc aaagttgatc ctaactcact ttaatcaata aaaaactagg
     1381 aattccttcc tagttttatt tattctatat acttaactag ttaagtatat attttaataa
     1441 tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt ttatctgtat taaccttctt
     1501 aaagactaat gaatgtgcga tgtgttttaa gtaacagatg agaataagga aaaatgaaat
     1561 tggcgatatg gtatgtgact attatgatta ttctttagca ataatctatt aaaatgagtt
     1621 cgcagggaat aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag
     1681 acattactaa aatgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga
     1741 aagttggtaa aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact
     1801 ctgactgcat gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg
     1861 cttgcttggg gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt
     1921 gttatccagg atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa
     1981 ccagctttag cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg
     2041 agaaatgcta aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt
     2101 gatagtattt tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa
     2161 ttggaaaatt atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat
     2221 agtgctactt tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct
     2281 ttccctaaag gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa
     2341 tgggttgtga aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac
     2401 tggggaaaga aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg
     2461 cgtgctttag cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt
     2521 ttagacactt ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct
     2581 atgtcaacaa gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt
     2641 cgtcaggcaa tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga
     2701 acagaaaagc cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta
     2761 aatccttata gctataatgt tgataaagca aatcagctat tggatcaagc tggttggaag
     2821 atgggaaaag ataaggttcg tgaaaaagat ggtaaagctc taaccttacg tcttccttat
     2881 atcgccacta aggcaactga taaagacctg gtaacttatt tccaaggaga atggcgtaaa
     2941 atcggtatta atgtttctct tattgctatg gaagaagatg attattgggc taatgctaaa
     3001 aagggtaatt ttgatatgat gttaacctat tcttggggag caccatggga ccctcatgct
     3061 tggatgtcgg cattaactgc caaggcagat catggacatc cagaaaatat agctttagaa
     3121 aatctagcaa ctaaaactga aatggataga cttattaagt cagctctagt agatcctaag
     3181 gaagaaaatg ttgatagaga ctataagaag gttcttgaat tattgcatga tgaggctgta
     3241 tatatcccat taacttatca atcggtgatt tctgtttata ggaaaggtga ttttaaaacc
     3301 atgcgttttg cgccagaaga aaattcattc ccattacgct atatagaaaa aaacaatgta
     3361 tctaagtagt tcgatcatca aaaaaatatt gtcagctttt ttggcattgt ttttcatatc
     3421 acttctaaca tttattctta tcaaactatc aacagttaat ccagcagaaa attaccttcg
     3481 tttatcaaaa ataagtgtta gtccagaagc gctaaaagaa gcagagcact atttaggttt
     3541 agataagcca ttatggaagc agtactggct gtggttccaa aaagcattaa caggagattt
     3601 tggatattct tatgtgctca ggttgccagt attagatttg gttttacaac ggtttttggc
     3661 tactttattt ttaggcacga gtgccttctt gctaattgtt accatttcta ccccgttagg
     3721 tgtgtgggct ggcttacatg aatctgctcg gagtgatcac ttgattcgat ttttgagttt
     3781 ttctagtgtg tctatgccaa atttttgggt tgcttacttg ttgatgctgc tattttcggc
     3841 aaaattaaat cttctgcctg tttcaggagg taacgattta caaagtttaa ttttgccaag
     3901 tattacctta agtttttcta ccgtaggtca atacatagcc cttattcgaa aagctattag
     3961 tcaagaaaat agaagtttaa atgtggaaaa tgcccgatta agaggagtaa aagaacgtta
     4021 tattgtcaca catcacctcc ttagaaatgc cttacctgcg ataatgacag cacttagctt
     4081 gacttgggtt tacttattaa caggatcaat tattgttgaa gaaatttttt catggaatgg
     4141 aataggacgt ttatttgtga ccagtctaag aacttcagat cttccagtaa tacaagcttg
     4201 tatgctaatt tttggaacct tatttttagc taataatttt atgacacagt gttttatgaa
     4261 ttgggttgat cctcggttac gaaagtcaag ggagaaataa tatcaaaaag aatttatttt
     4321 tcgagtagct tactggtatt gttagtgatt agtgccattt ttgcccctat tctatctagc
     4381 tttgatccac aatacgtaga tttatcacaa aaattattgg ctcccaataa tgttcatttg
     4441 ttagggactg accaattggg tagagatgta ttatctcgct tgctttatgg tgctagatat
     4501 tcactgtttt tagctattat tattagctta ttggaattaa cgattggtat gtttgtgggt
     4561 cttattgttg gttggtatca aggaaagtta gaaaacctct ttttatggat agctaatatt
     4621 attttagctt ttccgagctt tttgctctct cttgcaaccg tggggatctt aggtcatggt
     4681 ctagggaatt taatttttgc aattgtcttt gtggaatggg tttactatgc taaattaatg
     4741 accaatttag ttaagagtgc aaaaaaagag ccttatgtga taaatgcgca aattatggga
     4801 ctttcagtct ggcatatttt aagaaaacat atttttcctt ttgtgtatca gccaatcctc
     4861 gttatggtcc ttatgaatat aggaaatatt attttaatga tttctggctt ttctttctta
     4921 gggattggtg tgcaacctaa tgtcacagaa tggggaatga tgttgcacga tgctagagga
     4981 tattttagga cagctacttg gatgatgtta tctcctggaa ttgcgatttt tttaacagta
     5041 ttttccttta atactttagg cgatgccata gataaaaaag attggaaacg acaatggaac
     5101 agttagaaat aagaaaattg tcattacaga taggagaggt ccctgtacta agagatttta
     5161 gttgtaaaat agatatggga gaatcgctaa ctattattgg tgagagtggt tcagggaaaa
     5221 ccctcttggc aaaattattg gttggtcaca ttccacaagg tatgacagtt agaggaaaca
     5281 tattttttaa gggagttgat ttaggtaaac taactgtaaa gcagtggcag aaattaaggg
     5341 gacgagatat tgcctattta gtgcaaaatc ccatgtctat gtttaatcct tttcaaaaaa
     5401 ttgaagcgca tattttggaa acaatcctta gtcatgagaa atgttcaaag agagtagcct
     5461 tatctaaagc gttagaatgg atgaaacgtt taaatttaga tgatgcgata tccctgttaa
     5521 aaaaataccc ttttgagctt agtggaggaa tgctacaaag aattatgtta gccactatat
     5581 tatctcttga tcctcaggta atcatattag atgagccgac ctcagcggtt gattgtcata
     5641 attgttctac tatatcagct attttgcagg aattgcaaaa taatggaaaa accttgatta
     5701 ctgtaacgca tgattatcag ttagctagag acctgggggg acaattatta gttataagtg
     5761 agggagaagt tgttgaacag ggacaaaccc aagctatttt aagcaatcct caacataact
     5821 atacgaaagc actgacagtg caaatggagt atgaaggaga tattcttaat gttggtttgt
     5881 aatcatgtcg gaaaaacatt tggacgacaa gaggttttaa aggactgtca ttttcatctt
     5941 aaaagaggag aaataattgg tataatgggt aaaagtggca gtggtaaaag tagccttgcc
     6001 cgactcatta taggactaga tagtcctact tgtggttcga tatattttca aggaaaaatt
     6061 tacacaccta aagatggtaa ggcgcagatt atccttgttt ttcaagatgc gcttagttcg
     6121 gtcaatccat attttagtat tgaggaaatt ttgaatgaag ctttttatgg aaaaaaaaca
     6181 acttttgaat tatgtcaaat attagaagcg gtgggtttag atggaactta tctaaaatat
     6241 aaagctagac aacttagtgg tggtcagcta cagagagttt gtatcgctag agctttactc
     6301 ttaaagccta aaattattat ttttgatgaa tccttaagtg gattagaccc agtaactcaa
     6361 attaaaatgc tacgtttatt acaaaaaata aaacgccgtt atgaattaag ttttataatg
     6421 atttctcatg accctaaaat ttgtcaagct atctgtaacc gggtctttct gataaaaaat
     6481 ggttatttag tagaagataa tgaattcttg aagagagcgt gttctactaa ctgtttgacc
     6541 aatctctgaa ttattatgtt tttcttattt taggagttga taaagaaatc aacttactag
     6601 tgtcattgct ctattaaaat tttgcctttt atggtaaaat aataaagaca aaataaggag
     6661 aacatagtag tggaacctaa atatcaacgt atattaatta agttatctgg tgaagcattg
     6721 gcgggagata agggtgtcgg tattgacatt cctaccgttc aatctattgc aaaagaaatt
     6781 gccgaagtac acaattcagg tgttcaaata gcacttgtca ttggtggagg taatctttgg
     6841 cgtggagaac ctgcagcaga agcagggatg gatcgtgttc aagctgatta tacagggatg
     6901 ttagggacag taatgaatgc tcttgtaatg gctgatagcc ttcaacaata tggggtagat
     6961 actagagttc aaacagctat tccaatgcaa actgttgctg agccttacgt acgtggtcgt
     7021 gctttacgtc atctagagaa gaatcgtatt gttgtctttg gagcgggtat tggttcgcct
     7081 tatttctcaa ctgatacaac agcagcgctt cgtgctgctg agattgaggc agaagcaatt
     7141 ttaatggcta aaaatggagt agatggtgtt tataatgctg atccaaagaa agatgccaat
     7201 gctgtcaaat ttgatgaatt aactcacgtt gaagtaataa aacgagggtt aaaaatcatg
     7261 gatgcaaccg catcaactat ttcaatggat aatgatattg accttgttgt tttcaatatg
     7321 aatgagactg gtaatattaa gcgtgttgtt cttggagaac aaatcggaac aactgtttca
     7381 aacaaagcat ctgaatagga gtgtataaaa aatatgacta aggaaatcgt tacaaaagct
     7441 caagagcgtt ttgagcaatc acaccaaagt ttatcaagag aatttgcagg gattcgtgca
     7501 ggacgtgcta atgctagctt gcttgatcgt attcaagttg agtattatgg agcgccaacc
     7561 ccacttaacc aattagcttc tattactgta cctgaagctc gtgttctttt aatttcacca
     7621 tttgataaat catcaatcaa agatattgag cgtgctatta acgaatcaga tttaggtatt
     7681 aatcctgcaa atgatggttc agttattcgt cttgttattc cagctttgac agaagaaaca
     7741 cgtcgtgatt tagctaaaga agtgaaaaaa gtcggagaaa atgctaagat tgctatccgt
     7801 aatattcgcc gtgatgctat ggatgaagct aagaagcaag aaaagaataa agaaattaca
     7861 gaagatgact tgaaatctct tgaaaaagat attcaaaagg ctactgatga tgctgtaaaa
     7921 catattgatg agatgactgc taataaagaa aaagaattgt tggaagttta atagtaacgt
     7981 ttgctatctt tattatgatt ggcaagtaat aaaatgatta tggaggtaac tgatagaatc
     8041 aaaaccagat ggatttggtt tgtcggttac ctttttaaaa ggagaaaaat gaatacatta
     8101 ttagcgacag tcataacagg cttagtaact gatgaaaaca aagattttta ctttatccaa
     8161 aaagatggct ttacctttgc attatcaaag tcagaaggag agcatcacat tggtgaaatg
     8221 gttaaaggat tcgcctatac agatatgcaa caaaaagcac gtcttacaac taaagaaaca
     8281 tttgcgactc gagatcatta tggttggggg acagttactg aggtacgtaa agacttggga
     8341 gtatttcttg atactggtct tccagataag caagtggttg tttctttgga tgttcttcct
     8401 gaattgaaag aactttggcc taaaaaaggt gatcgcctct acgtttgctt agatgttgat
     8461 aaaaaagatc gtctctgggc tctccctgca gatcctgaag tgtttcaacg aatggcaacc
     8521 cctgcatata acaatatgca aaatcaaaat tggccagcaa tcgtttatcg cttaaaattg
     8581 tcaggaactt ttgtttatct accagaaaat aatatgctcg gctttattca ccctagtgaa
     8641 cgttacagtg aacctcgttt agggcaagtg ctggatgcac gagttattgg cttcagagaa
     8701 gttgatagaa ccttaaattt atcattaaag cctcgttctt ttgaaatgtt agaaaatgac
     8761 gcacaaatga ttttaacgta tttagaaagt aacggaggat tcatgacttt aaatgataaa
     8821 tcttctccag aggaaattaa agctactttt ggaatttcca aaggtcagtt taaaaaagca
     8881 cttggaggac tcatgaaagc taaaaagatc aaacaagatc agctaggtac agaattatta
     8941 taaacttttg aacttttaaa gttttctcac gaggtcgttt tagttatagc tagaattaat
     9001 ggctaattat agaaaaaagc tgagaggaaa ttttggcttt ttttgttata atgtatctat
     9061 taaaaagaat ggagaacgat atggaacgtg cgatttttgc tggaggttgt ttttggtgta
     9121 tggtacaacc atttgaagaa ttagatggta ttgaatctgt tttatctgga tacactggag
     9181 gacatgttga gaatccaact tataaagaag tttgtagtaa aactactgga catacagaag
     9241 ctgttgaaat tatttttaat cctgaaaaaa tctcttatgc agatttagtt gagttatatt
     9301 gggcacaaac agatccaaca gacgcttttg gtcagtttga agatcgtggg gataattatc
     9361 gtcctgtcat cttttatgag aatgaagagc aacgtcagat tgctcaaaaa tcaaaagata
     9421 agctacaagc ttcgggtaga tttgatagac caattgtaac cagcattgag cctgctgata
     9481 ctttttaccc agcagaagac tatcatcaag cattctatcg gactaaccct gctcgttacg
     9541 ccttgagtag tgccagaaga catgcatttc tagaggagaa ctggcattga gaaaatcatt
     9601 ttacagttgg ttgatgacac aaagaaatcc aaaatcaaat gaaccagtag caatcctagc
     9661 agattacgct tttgatgaaa ccacttttcc aaaacatagt tcggattttg aaacagttag
     9721 tcgctattta gaagatgagg ccagtttttc ctttaattta acagattttg atgatatttg
     9781 ggaagattat ctcaatcatt aacaataata tagagattgt attttcaatc tcttttttag
     9841 gttatagaaa ttggacacat tgtctaagac tgtctgaaaa gcaggttttt taatggcaat
     9901 cgttttcaca aaggagtaga atatagtcat ctaaaatata gccctaggga ggttgtttat
     9961 gagatataca aatggaaatt ttgaagcctt tgcaagacct cgaaaacctg aaggtgtgga
    10021 taaaaaatcc gcttatattg ttggttctgg tttagcagga ttagctgccg ctgtcttttt
    10081 aatacgtgac ggtcaaatgg atggtcaacg tattcatatt tttgaagaac tacctctttc
    10141 tggaggatca cttgacggtg tcaaacgacc tgatatcggt tttgtaacgc gtggtggtcg
    10201 tgaaatggaa aatcacttcg aatgtatgtg ggatatgtac cgttccatcc cctctctcga
    10261 agttccagat gcttcttatc tagatgaatt ttattggctt gacaaggatg atcccaattc
    10321 atctaactgt cgcctcattc ataaacaggg gaatcgctta gaatctgatg gtgattttac
    10381 actcggaaca cattccaaag agttagttaa gctagtcatg gagactgaag agtctttagg
    10441 tgctaagacg attgaagaag ttttttcaaa agaatttttt gaaagtaatt tttggactta
    10501 ttgggctact atgtttgcct ttgagaaatg gcattcagcg attgaaatgc gtcgatatgc
    10561 tatgcgcttt atccatcata ttggtggtct gcctgatttc acttcattaa aatttaataa
    10621 atataatcaa tatgattcta tggtgaaacc aatcatcagt tatttagagt ctcataatgt
    10681 agatgttcaa tttgatagca aggtaactaa tatctccgta gactttaaga acggtcagaa
    10741 gcttgcaaaa gccattcatc tcacagttgg tggtgaagca aaaacaattg atcttactcc
    10801 aaatgatttt gtttttgtaa cgaatggttc tatcactgaa agtacgaact atggtagtca
    10861 tgatactgta gctaaaccta ataccgattt gggaggttct tggaatcttt gggaaaactt
    10921 agctgctcaa tctgatgaat ttggacatcc taaagtcttc tacaaagata ttccaaaaga
    10981 gtcatggttt gtttctgcta ctgcaacaat aaaagatcca gctattgaac cttatataga
    11041 gcgtttaaca catcgtgacc tccatgatgg taaagtaaat accggaggca ttgttacagt
    11101 tacagattct aattggatga tgagttttgc tatccatcgc caaccgcatt tcaaagagca
    11161 aaaggaaaat gaaacaattg tttggattta tggactctat tcaaatgtgg aaggtaatta
    11221 tattaaaaaa ccaattgaag aatgtactgg tcgagaaatc acagaagagt ggttatatca
    11281 cttaggtgtt cctgaaatga agattcatga tttatctgat aaacaatatg taagtacagt
    11341 tcctgtttat atgccttaca ttactagtta tttcatgcct cgagttaaag gtgaccgacc
    11401 agatgttatc ccacaaggat ctgttaacct agccttcatt ggtaattttg cagaatctcc
    11461 atccagagat acagtattta caacggaata ttctattaga actgctatgg aagctgttta
    11521 tactttctta aatattgaac gtggtgttcc agaagtcttt aattcagcat ttgatattcg
    11581 tgtcttgttg caatctttat actatctcaa tgataagaaa tctgttgaag atatggattt
    11641 accaatacct gccttaatgc gtaaagtagg catgaaaaaa attagaggaa cttatttaga
    11701 agaattactt cgagaagctc atcttttata atgtaaaata aaatcgagaa aacttgtttt
    11761 tctcgatttt ttagttttac aacgactaaa atcaccaaag aaaatgaaat tttgttataa
    11821 tgtagtcact tactgtacga aaaggaagaa taatttgcaa gaatactcta tcgaaattac
    11881 tttacaacat ccagatgata tgatgtcact ctttggcagc aatgagcgcc atttaaaatt
    11941 aatcgaagaa aatttagatg ttatcattca tgctagaact gaacgtgttc aggtattagg
    12001 tgactctgaa gaagcagtag aaaccgctcg gttaaccatt gaagcattat tagtgcttgt
    12061 taaccgcggg atgactgtga atacttcaga tgttgtaaca gccttatcca tggcacaaaa
    12121 tggttcaatt gataaattcg ttgcccttta tgaggaagaa attattaagg atagctatgg
    12181 taagcctatc cgtgttaaga ctttagggca aaaaatatac gttgatagcg ttaaaaatca
    12241 tgatgttgtt tttggtattg ggcctgctgg aacgggaaaa acttttttag cagtcactct
    12301 agcagtaacg gcactaaagc gtggtcaagt taaacgaata atcttaacga gaccagcggt
    12361 tgaagctggt gaaagccttg gtttcttgcc tggagatctc aaagagaaag ttgatcctta
    12421 tttgagacca gtttatgatg ccctatatca aattttaggt aaagagcaaa caagtcgctt
    12481 aatggagcgt gaaatcatcg aaattgctcc attggcttac atgcgtggtc gtactttaga
    12541 tgatgcattt gtcatattag atgaagcgca aaacaccact ataatgcaaa tgaaaatgtt
    12601 cctaacacgt cttggcttca attctaaaat gattgtaaat ggcgatgtta gccaaattga
    12661 cttaccaaag aatgttaaat ctggtttgat tgatgctgtt gagaaattac gaaatatcaa
    12721 aaagattgac tttattcatt tatcagcaaa ggatgttgta agacaccctg tcgttgctga
    12781 aataataaat gcttattcag attcagaaag tagtcacaag ctacaatctg ataaggactg
    12841 atgtttgcca ctaggagacg ttgttagaga gtgtgtcaat atgtaaaaaa cgcctaatgc
    12901 tttaggcgtt tttatgatgg tgtacttcaa cttaacctat aaagtgttga aaattttctt
    12961 taagcatgac gtcgagatgc attattatat cgtttaatgt ttctttaaca gtgtgttcgt
    13021 tggagttgtg atgacatagt ttaatgatat tttcttcgaa atgtttagga agcaatgtag
    13081 cattcttctt agcataggaa atcattcttt tttcgccagg atgtgtcaac ttattaagtg
    13141 caaaaataat atcaaagtag gaatctaaga aagcagtagt tctatggtga gtactaacaa
    13201 agtcttggcg tttaagggct tttataattt gtttatcgta agagggaagc ttgccagtta
    13261 ataagttacg attttgaatg ataatttgtt tttgtaactg actgggataa gaaacttcga
    13321 atctctcttt gagtgattgt aattgatttt caggatcata gagtatttgg caattgatga
    13381 ggttatgcca aaaacaagta gtgtatccaa ttcgagaatt gtggtgttca acgacatctt
    13441 ctaagtctga taaaaagtta tctatgttac gatagagaat atcaatatca gttccgtcgt
    13501 ttaaagtacc attatcttca agctcccagt aatgattgtt aagttcaatg taatgacagt
    13561 gcttgttaag gatacgttta cgactagtga tatcaggagt tgctgcacaa taaacataca
    13621 aatcataatc ggaagattgg tcaaaactgt caccagagcg tgagcctccg agtgctatag
    13681 caacaacatt tggaagttta gagaaatctt gccagagttt tgtgaaatgg tccataaatg
    13741 ctctcctaaa aatgtaaaat attgatgtgt ctccattcta cagggctatc tctttttagt
    13801 caataattaa tagtaagtta ataaaagagg taacggttct aatattcaag aagtgtatat
    13861 gagtgatttg aaaaggagat attttatgct acaatacgta catattagtt taaacaaggt
    13921 gatagaatga aacaagatta tatttcctac attaggtcaa aagttggaca tgagactatc
    13981 tttttaacat attctggtgg tattttgaca gatggtaagg gacgtgtctt actccaatta
    14041 agagctgata agaattcttg gggaattatt ggtggacgta tggagttagg tgagtcatca
    14101 gtagatacat tgaaaagaga atttttcgaa gaaactggat tgagagtgga accaatcaga
    14161 ctgttaaatg tttatactaa ctttcaagac tcttatccca atggcgataa agcacaaacc
    14221 gtcggtttta tttatgaagt aagctgtcca aaaccagtta atatagaagg ttttcataat
    14281 gaggaaacat tgcaattgga ctatttttct aaagaagacg tcaaaaatat tacgatcgta
    14341 aatgaacaac accaattaat tttagatgaa tatttttcac aaacattcca aatggggcgt
    14401 taaaaggatg ggaaatacta ggtgtgactt taaagaactt ctaactttta ttgatatgtt
    14461 gttaggcatt cttgtagaaa caaatgttaa gaaaataagg agttctcatt tgtatatttt
    14521 ataattatta aaaagaaaaa cagtagcttg caataattta gtgactgttt ttttagaaag
    14581 cataagaagt acagaaaaag aaaattatgg aatattggat ggtggggata tgctttactt
    14641 acggtattgg aatgatatac ttaaagaaat ccagttttag aaatccaaat ttctaagtaa
    14701 aatagcataa aggagagtag atgaatgaag ataacattac acggcgttgc tgaaacgtta
    14761 ttaatcacct tatatattag ggctaaagat gcgatggcta aacatccgat acttaatgat
    14821 caaaaatcct tagcaattgt tgaacagata gaatatgatt ttgataaatt cgataattca
    14881 gaagcttctt tttatgcaac attagctaga attcgcgtta tggatagaga aatcaaaaaa
    14941 tttattagag aaaatccaaa tagtcaaatc ctttcaattg gttgtggact tgatacaagg
    15001 tttgaaagag tcgataatgg acaaattagg tggtataacc ttgatttgcc agaggttatg
    15061 gagataagaa aattattttt tgaagagcat gaaagagtta ctaatatagc aaaatcagcc
    15121 ctagatgaaa cttggacacg ggaggtaaat ccccaaaatg ccccttttct aatcgtgtca
    15181 gaaggtgttt taatgtttct aaaagaagat gacgtagaga cttttcttca tatcctgaca
    15241 aattcattta gccaatttat ggcacaattt gatttgtgtc ataaggaaat gattaataaa
    15301 ggaaagcaac atgatacagt aaagtatatg gatacagaat ttcagtttgg tatcacagat
    15361 ggtcatgaga ttgtggattt agaccctaaa ttaaagcaaa taaatctgat taactttaca
    15421 gatgagatga gcaaatttga gttaggcaca cttcgctctt tacttccaac aattcgtaaa
    15481 tttaataatt gtttaggtgt gtacgaatat aaagcatctg agaaaaagta acaaaaatct
    15541 atttatataa agagacattc taaatgtaaa tttgtcataa tcgttatagc atttgtagct
    15601 ttgtttattt aggaattagt tttacgtaag ttaggcgctc taaagtttgt attttgaagt
    15661 aaatactaat tcataagtca gaattttatt gaaatggatt ataagtgtgt aatagtgatt
    15721 attagctttc tctccctttt ttaatactct tatggtataa tgatgtgaaa gaatagcttg
    15781 aaggaaggaa gagaacgcac ttttattttg agaactagtt ttagataaaa gtgaaaaact
    15841 taggaaagta gatgtgtcga ttatttagaa ttatgcatta cactttatct tttctaaacg
    15901 ttttgggtcg ttcttactat ataagatgta cgttgaaatg attgatgaaa ctggacaagt
    15961 atcagaagat atcaaaaagc aaactcttga tttgttagag tttgcagcac aaaaaacagg
    16021 taaagagaat aaagaaatgg ctgtaacgtt tgtcactaat gaacgtagcc atgaattgaa
    16081 tttagagtat cgagataccg atcgtccgac tgatgttatc agtttagaat ataagcctga
    16141 ggttgatatt tcttttgatg aagaagatct ggctgaaaat cctgaacttg ctgagatgct
    16201 agaagatttt gattcttata ttggtgaact ttttatttct attgataaag ctaaagaaca
    16261 agcagaagag tacggtcact cttatgaacg tgaaatgggt tttttagctg tacatggttt
    16321 tttgcatatt aacggttatg atcattatac acccgaagaa gaaaaagaga tgttcagctt
    16381 acaggaagaa attttaactg cttatggact taaacgacaa taatcaaaaa aaatggaaaa
    16441 atcgcacctt aacatcaagc atggaatttg ctgttacagg gatttttaca gcttttaaag
    16501 aagaaagaaa tatgcgtaag catttggttt ctgccatttt agtaatactt gctggtctta
    16561 cttttcaagt atctatggtg gagtggttat ttcttttatt gagtattttt ttggtaatta
    16621 cttttgaaat tatcaattca gctattgaaa atgtagttga tttggcaagt aattaccatt
    16681 tctcgatgct ggctaaaaat gctaaagata tggcagcagg agcggtttta gttgtttcct
    16741 tatttgccgt attggtggga ttgattattt ttatacccaa gatacttgct ttacttttat
    16801 gatgagaagt attacgaata aattgaatgg agaaagaaaa gaaatgacat ttaagtcagg
    16861 atttgtagca attttaggtc gtccaaatgt tggaaaatca accttcctaa accatgttat
    16921 gggacaaaaa attgctatca tgagtgataa agctcaaaca acacgtaata aaattatggg
    16981 gatttataca acagaaactg agcaaattgt tttcattgat accccaggaa ttcataaacc
    17041 aaagaccgcc cttggtgatt ttatggttga atctgcttat agtactttac gtgaagttga
    17101 aacagttctt ttcatggttc ctgccgatga aaagcgaggc aaaggcgatg atatgattat
    17161 tgaacgtctt aaggctgcca agattcctgt tattttagta atcaacaaaa ttgataaggt
    17221 tcatccagac caacttttgg aacagattga tgacttccgt agtcaaatgg attttaaaga
    17281 agtagtgcca atttctgccc ttcaaggaaa taatgtgcca accctaatca agttattgac
    17341 tgataatctt gaagaaggtt tccaatattt cccagaagat cagattactg atcatccgga
    17401 acgattttta gtatcagaaa tggttcgtga gaaggtactt catttaacgc aacaagaggt
    17461 tcctcactca gtagctgttg ttgtcgaatc catgaaacgt gatgaagaaa ctgataaagt
    17521 gcatattcgt gcaactatca tggttgaacg tgatagtcaa aaaggcatta tcattggaaa
    17581 acaaggtgct atgctcaaga aaataggaaa aatggcgcgc cgtgatattg aacttatgtt
    17641 aggtgataaa gtttacctag agacttgggt aaaagtcaag aaaaactggc gtgataaaaa
    17701 gcttgattta gctgattttg gctataatga aaaagagtat taatgagttt attttgagat
    17761 ccgcaagtta gtggggaaga gttagataag ttcaccgtga gacttgattc gcacagaaaa
    17821 agcaattcac gaggataaaa ccaactagtc ataattttga gattatattc acaaattaat
    17881 taaagggagc caaacatgct tgttggcccc ttcttaaata aaggagagca catgactcaa
    17941 gattatattt gctacctata aagtagagga ggtgaggata tgcataatat attaagattt
    18001 ttaggcattg ttattatttc tgcagtgatt ctatttagta taggtagttt ctacgattta
    18061 tcattaatga aaaatatact acttatctgt tggagttttc tctttgatct attggttttt
    18121 gtcttcaaac aaagacaaac gacagaagta ctcacatggt atcaagttgt taagcaattt
    18181 tggttattta ttaagtgtac tattcttatt cctatcttag ttgcttttat tataatgaaa
    18241 ggatgtttga caagtat
//
LOCUS       DATRME010000036        18255 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRME010000036 DATRME010000000
VERSION     DATRME010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18255
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            62..1321
                     /locus_tag="VBC23_001213"
     CDS             62..1321
                     /locus_tag="VBC23_001213"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6843163.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            1423..1977
                     /locus_tag="VBC23_001214"
     CDS             1423..1977
                     /locus_tag="VBC23_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843164.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            1970..3253
                     /locus_tag="VBC23_001215"
     CDS             1970..3253
                     /locus_tag="VBC23_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6843165.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            3276..4136
                     /locus_tag="VBC23_001216"
     CDS             3276..4136
                     /locus_tag="VBC23_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6843166.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            4138..5058
                     /locus_tag="VBC23_001217"
     CDS             4138..5058
                     /locus_tag="VBC23_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6843167.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(5075..5530)
                     /locus_tag="VBC23_001218"
     CDS             complement(5075..5530)
                     /locus_tag="VBC23_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6843168.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      5601..5714
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            5712..6221
                     /locus_tag="VBC23_001219"
     CDS             5712..6221
                     /locus_tag="VBC23_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6843169.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            6361..8319
                     /gene="ligA"
                     /locus_tag="VBC23_001220"
     CDS             6361..8319
                     /gene="ligA"
                     /locus_tag="VBC23_001220"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6843170.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            8331..9350
                     /locus_tag="VBC23_001221"
     CDS             8331..9350
                     /locus_tag="VBC23_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6843171.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            9354..11654
                     /gene="pulA"
                     /locus_tag="VBC23_001222"
     CDS             9354..11654
                     /gene="pulA"
                     /locus_tag="VBC23_001222"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6843172.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            11859..13727
                     /gene="glgB"
                     /locus_tag="VBC23_001223"
     CDS             11859..13727
                     /gene="glgB"
                     /locus_tag="VBC23_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO6843173.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            13769..14908
                     /locus_tag="VBC23_001224"
     CDS             13769..14908
                     /locus_tag="VBC23_001224"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6843174.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYFESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            14898..16031
                     /gene="glgD"
                     /locus_tag="VBC23_001225"
                     /pseudo
     CDS             14898..16031
                     /gene="glgD"
                     /locus_tag="VBC23_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            16028..17458
                     /gene="glgA"
                     /locus_tag="VBC23_001226"
     CDS             16028..17458
                     /gene="glgA"
                     /locus_tag="VBC23_001226"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO6843175.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            17790..17990
                     /locus_tag="VBC23_001227"
     CDS             17790..17990
                     /locus_tag="VBC23_001227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6843176.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            18022..>18255
                     /locus_tag="VBC23_001228"
     CDS             18022..>18255
                     /locus_tag="VBC23_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6843177.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLL"
BASE COUNT         5939 a         2722 c         3732 g         5862 t
ORIGIN      
        1 aagaatttaa actatgataa tatagtagag ttgaattcta gttaaagaaa gatttgaaag
       61 tatgcgtaaa attattatta acggtggcaa gcaactgacg ggagaagttg ctgtatcggg
      121 tgccaaaaat agtgtagttg ccttgattcc tgccactatt cttgctgatg atgttgtggt
      181 cttagatgga gttccggcta tttctgatgt tgacagttta gttgacatta tggaaactat
      241 gggagctaag attaagcgtt atggtgagac gcttgaaatt gatccacgtg gcgttaaaga
      301 tattccaatg ccttatggca aaatcaatag tcttcgtgca tcctattatt tctatggaag
      361 cttattgggg cgttatggtc aagcaacatt aggtttacct ggtggttgtg atttaggccc
      421 ccgtccgatt gatttacatt taaaagcttt tgaagcgatg ggagctagtg tttcttatga
      481 aggtgattca atgcgtctag caacaaacgg aaagcctttg caaggagcaa atatttatat
      541 ggatactgtc agtgttggtg cgacaattaa cactatgatc gcagcagcta aggcaaatgg
      601 tcgtacggtt attgaaaatg ctgcgcgtga accagaaatt attgacgtgg ctactctttt
      661 aaataatatg ggagctcata ttcgtggtgc tgggactgat gttattacaa ttgaaggtgt
      721 gaaatcgctc catggcacgc gtcatcaagt aattccagat cgtattgaag caggaactta
      781 tattgctatg gcagcagcta tcggacgagg aataaaagtg accaatgtgt tatatgaaca
      841 ccttgagagt ttcattgcta agttagatga gatgggagtt cgaatgacag ttgaagagga
      901 ttcaattttt gtcgaagaac aagaaaagtt aaaagctgta tccataaaaa cctcacctta
      961 cccaggattt gctacagatt tacaacaacc tctaacccct ttattactaa cagcagaagg
     1021 aaacgggagt ttattagata ctatctatga gaaacgtgtt aatcatgttc ccgaattagc
     1081 acgtatggga gctaatattt ctaccctagg gggcaaaata gtttatagtg gtcctaatca
     1141 attatctggt gcacctgtaa aagcgacaga tttgcgtgca ggcgctgctt tagttatcgc
     1201 tggcttaatg gcagagggac gaactgaaat tacgaatatc gagtttattt tacgtggata
     1261 ttctaatatc atcgagaaac taacatcatt gggtgcggat atccaattgg ttgaggaata
     1321 gtatatataa caaagagttg agaatatttc ttagctcttt tcttcttgtt ttagattaag
     1381 tcacttatag gtatttgaaa tctcttgtgg tataatatta tcatggatat atggacaaat
     1441 ttaggacggt ttgcattcat tgaaacagaa catgttaacc tacgtcctgt tgcctacact
     1501 gatagggaag ctttttggcg aatagcttca aaaaggacaa atttacaatt tatttttcct
     1561 gttcaaacca gtaagaagga aagtgatttt cttttagttc attcttttat gaaagagccg
     1621 ttgggagttt gggcgattga agataaagtt agtcataaaa tgtttggagt tatccgtttt
     1681 gaaaacattg atttatctaa aaagacagct gaaattggct attttttgaa agaaagttct
     1741 tggggtcaag gcattatgac tgaatgtcta aagacacttt ctttttttgc ttttcaagag
     1801 tttgggatgg acaaattgat catagtaaca cataaagaga atattgctag tcagaaggtt
     1861 gctttgaagg cgcatttcaa acaatctagg agttttaaag gaagcgaccg ttatacacgc
     1921 cgtataagag actatattga gtttcaatta actaggggtg attatcgtta tgagtaaaca
     1981 tcaagaaatt ttggagtacc tagaaaattt agctgttggt aagagggtta gtgtacgcag
     2041 tatttcaaat catttaaagg tgagtgatgg cactgcatac cgcgctataa aagaggcaga
     2101 aaataggggt attgttgaga ctagaccgcg tagtgggaca gtacgtgttg ctcaaaaagc
     2161 aaaggttaat attgaaaaac tgacttatgc tgaaattgca aggattagtg actcacaagt
     2221 tgttgcagga acagaggggc tcagcaagga gtttagtaaa ttttctatag gtgcaatgac
     2281 tcacagaaat attgaaaaat atttggtgca aggagggtta ttgattgttg gcgaccgtga
     2341 tgaaatacaa catttagcct tacaacatca aaacgctatc ttagttacag gtggctttaa
     2401 tgtttcccca tctgtttgtc gtttagcaga taaacttcaa attcccgtaa tggtaacaca
     2461 ctatgataca ttcacagtat caacgatgat taatcacact ttgtcaaatg ccaaaattcg
     2521 tactgattta aagaccgttg aacaagtcta tcagtcgcaa atggattacg gtttcttagc
     2581 gcaggatgat accgtaaaag aatttaatct tcttgttaaa caaactaaaa atgttcgttt
     2641 tccaatagtc aaccaagcta atgttgtcgt tggtgttgtt agtgtgcaag atattcttgg
     2701 aaaagataaa gaagtaaaat tggcaacagt catgtcaaaa aatatcattg ttgctaaacc
     2761 tcgaatgagt ctagctaata tcagtcaaaa aatgattttt gaagatttga atatgatgcc
     2821 agtagtatcg gatgactttg aattattagg cgttattaca agacgacaag ctgtagaaaa
     2881 tttatcgatg tcgcaaggga cagctttata cacctatagt gatcaaatct tgtctaattt
     2941 acaaattgaa gatggtcact tttcgttttt agtagaaccc gctatgattg accatacagg
     3001 tagcttaaca caaggagttt tgacggagtt cttaaaagaa atttgtattc gtgtcttaac
     3061 tcgtaagcat caacgcagta ttgttgttaa acaaatgaca ctttatttct tgcaacctgt
     3121 tcaaattgat gaaataataa tggtaacgcc aacaattatt tcagaaaaac ggcgcgaagc
     3181 tacactcgat ttagaattaa aattagaaaa taaaattatt gctaaagcta tgatagcggt
     3241 aaaaattaat tagatataag gagaataaat agaaaatgat tacattaaag tcagcacgcg
     3301 aaattgaagc aatggataga gcaggtgatt ttttagccag catccacatt ggacttcgtg
     3361 atattatcaa accaggagtt gatatgtggg aagttgaaga atacgttcgt agacgttgta
     3421 aagaagaaaa cgtattgcca ttgcaaatcg gggttgatgg tgcagttatg gattacccat
     3481 atgctacttg ttgtgggctt aatgatgaag tggctcatgc ctttcctcgt cattataccc
     3541 taaaacaagg tgacctttta aaagtagata tggttttaag cgaaccttta gataaatcaa
     3601 ttgttgatgt ttcatcgtta aactttgaca acgtggctca aatgaaaaag tacacggaaa
     3661 catatagtgg tggtttggca gactcatgtt gggcttatgc tgtaggtgaa gtttcacaag
     3721 aggtgaaaga cttaatgtct gtgacaagag aagcaatgta tattgggatt gaaaaagcag
     3781 ttatcggaaa ccgtattggt gatattggag ctgctattca ggattatgca gagagtcgtg
     3841 gttatggagt tgttcgtgat ttggttggtc acggtgttgg tccaacaatg catgaagagc
     3901 ctatggtgcc taactatggt acagcaggtc gaggtctccg tcttcgtgag gggatggttt
     3961 taaccatcga accaatgatt aatactggta cttgggaaat tgatacagac atgaaaactg
     4021 gttgggcaca taaaacctta gatggtggtc tttcttgtca atatgaacat caatttgtta
     4081 tcactaaaga tggtcctgtt attttaacga gtcaaggaga agaaagaact tactaaaatg
     4141 aaattaaaaa aattctttga ggatttgctg gctaaactag aatatagacc cattcaagtt
     4201 tttatgcgtc attttcaaag tgcagagatg gacttatcag ccatagcagt tgcttattat
     4261 ctattagtga cagcttttcc attactagtt attgctgcta atatttttcc ttattttcat
     4321 attaatgtgt cggatttatt gtcacttatg caaaaaaatc ttccgaaaaa tatttatgaa
     4381 cctgcctcac gcttagcagt agatgctttt tcgaaaccgt ctaccggcat tttgggattc
     4441 gcgtcattaa cagctttttg gacaatgtct aaaagtttga cctctcttca aaaagccatt
     4501 aataaagcat acggtgttga ccaacacaga gactttgtga ttagtcgttt agtgggtgtg
     4561 gggactgggc tgatcatcct atttttactg acttttgttt taattttttc aaccttttca
     4621 aaaccagttt tacagataat tgtaaacatg tatgatttgg gagatactct aacggcgtgg
     4681 cttttaaatc ttgcacaacc agttacattt ttgactatat ttttaggtat tggaatttta
     4741 tactttattt tacctaacgc acgaattaga aaagtacgtt atgtcattcc agggacatta
     4801 ttttcaacgt ttgttattgg tttttttagt aatttaatta gccaatacgt cttaaatagg
     4861 gttgaaaaaa tggtagatat taaaacgttt ggttcggtcg ttatctttat cttaatgcta
     4921 tggtttattt tcttagccca cattatgatt ttaggtgcaa tactgaatgc cagcgttcaa
     4981 gaaattgcga caggaaaaat tgaatcaagg cgtggagata ttatgtctct tatccaaaaa
     5041 tccaaagaag aaaaataaga aaagacagct cttgttagag ttgccttttt ttgtctttaa
     5101 aaataacaaa tttgctcaag atgtagttga gtaaaataat taatatttga gatacaaggc
     5161 tttcaatggt gttgatttta tttaaattgt gctgaacgaa ttgtccgata atagaaggaa
     5221 attgatcaac aaaaataaca gctaatatca aatctaatcc taatgtgcct aatctagcga
     5281 tgacaaatgt ttgtaagcgt tgaaactgtc caattttagt ttgtttaaaa acaaatcgat
     5341 catttgtaaa aaaagcaaaa agtatagcta ttatgttagc aactattgca gagaatggag
     5401 cgtcctgcga gaaattaaaa atcccttgtc gaataactaa ataaagaagg gatgttaata
     5461 gtccgaacag taaatataag ctgacttcat tttgaatact ttttttcagt aacgatttta
     5521 atttaaccat atttttatta tatcacggat gcttcaaagt gatgaggaaa tctcccataa
     5581 gcttggcttt actttttgtt tgtgatatac ttagtaagtt gttttacaca acccttgatg
     5641 cttagttccc tttcatcaag catattacaa acgttagaag tgttaactgt ctaaacgtta
     5701 aaggagaaat tatgaacaca tttacaactc gcgattacgc tcacatggct attgtaacag
     5761 ctatctatat cgttttaacc ataacgccac cttttaatgc tattgcttat ggtgcttatc
     5821 aattccgtgt ttcagaaatg ctaaatttct tggcctttta tcaccgaaaa tacctttttg
     5881 cggtaacgtt aggatgtatg atttctaatc tttatagttt tggaatgatt gatgtctttg
     5941 taggtggtgg ttcaacttta ctttttgttt atcttggaac cattttattc aagcaatatc
     6001 aaaaagatta tctatttaat ggccttatta acaaagcatt ctttttcttt tctttcttct
     6061 ttgctgcttc aatgattacg gtagcagttg aattgaaaat tgtagcagga ttacctttat
     6121 tattaacatg gttaacgaca gctgttggtg aattggcttc acttttggtt ggagcagtcc
     6181 ttgtggataa gttgagtaga cacgtcgatt ttacaaaata aatatattat aaagcgcgag
     6241 acattgatct cggctttttt gtttggtttt atagttagca tagctagaaa atgctgcaac
     6301 ttttatgaga aatgctccaa tttttattaa tatccttagt ctttgtgata aaatagaaat
     6361 atggaaaatc gtatgaatga attagtatct ttgttgaatc agtacgcaaa agagtattat
     6421 acacaagata atcccacagt ttctgacagt caatatgacc agttatatcg agagttagta
     6481 gaattagaag agcaacatcc agaaaacatt ttgccaaata gtcccacaca tcgagtgggt
     6541 ggtttagttt tagaaggctt tgaaaaatac caacatgaat atcctttgta ttcattgcag
     6601 gatgctttct caaaagaaga attgatagcc tttgataaac gtgttaaagc agagtttcca
     6661 actgcttctt atatggcaga gctgaaaatt gatggtctat ctgtctcgtt gacttatgta
     6721 aatggtattt tacaggttgg ggcaacacgt ggtgatggca atattggaga aaatattact
     6781 gaaaatttaa agcgtgttca tgatattcct ctacatttag accaaagttt agatattact
     6841 gttcgcggag agtgctacct tcccaaagaa tcttttgaag ctattaacat agaaaaacgt
     6901 gcaaatggtg agcaggaatt tgctaatcct aggaatgcag cagcaggaac cttgcgccaa
     6961 ttaaacacag ggattgttgc gaaacgtaaa ttagcaactt ttctttatca agaagctagc
     7021 cctacgcaaa aagaaacaca agatgatgtt ttaaaagagc tagaaagcta tggcttttca
     7081 gtcaatcatc atcgtttaat tagctcttct atggagaaaa tttgggattt tatccaaact
     7141 atagaaaaag atagaatttc cctcccttat gatatcgatg gtattgttat taaggttaat
     7201 agcttagcaa tgcaagaaga gttgggcttc actgtaaagg cacctcgttg ggcgattgca
     7261 tataaattcc ctgctgaaga aaaggaagca gaaatattat ctgttgattg gacagttggg
     7321 cgaacaggag ttgtaacacc aacagctaac ctaactccag ttcaactagc agggacaacc
     7381 gttagtaggg ccactctgca taatgtagat tatattgccg aaaaagatat ccgtattgga
     7441 gatactgttg ttgtatacaa agcaggggat atcatccctg cagtcttaaa tgtcgttatg
     7501 agtaagcgaa accagcaaga agtcatgctt attcctaaac tatgtccttc ttgtggtagt
     7561 gaattagttc attttgaaga tgaggtggct ttacgttgta ttaatccgct gtgccccaac
     7621 caaatcaaag aacgtttagc tcattttgct agccgggatg ctatgaatat tacaggtttt
     7681 ggtccatcct tagtcgaaaa attatttgat gctcatttaa tagcagatgt tgcagatatt
     7741 tatcgtttaa gtatagagga tttattaact ttagatggta ttaaagagaa atctgcaact
     7801 aagatttatc atgcaatcca atcttcaaaa gaaaattctg ctgaaaaact tctttttggc
     7861 ctaggtattc gtcatgtggg aagcaaggcg agccgccttt tattggagga atttggtaat
     7921 ttgaggcaac taagtcaagc aagtcaagaa agtattgcct caattgatgg actaggaggg
     7981 gttattgcca aatcacttca tacgttcttt gaaaaagaag aggtagataa actactagag
     8041 gaattaactt cctataacgt taattttaat tatctaggaa aacgagttag tacagatgca
     8101 caactttcag gtttaacagt cgttttaacc ggaaaacttg aaaagatgac tcgtaatgaa
     8161 gcaaaagaga aattacaaaa ccttggtgct aaggtgacag gtagcgtttc taaaaagact
     8221 gatttaattg ttgcaggaag cgatgcagga agtaaactaa ccaaggctca agatttaggg
     8281 attacaatac aagatgaaga ctggttactc aacctttaag tgaggaagaa atgaagaaac
     8341 aaaaaaaagc gcgtttaatt tataacccaa cttctggtca agaaattatg aaaaaaaatg
     8401 tagcggaagt tcttgatata cttgaaggat ttgggtatga aacatcagcc tttcaaacaa
     8461 cgccaactaa aaattctgcc agagatgaag ctgctagagc tgctcaagct ggttttgatt
     8521 tgattgttgc agcaggtgga gatggtacta ttaatgaagt tgttaatggt attgctcctt
     8581 tgaaacgtcg tcctaagatg gcaattattc caactggaac aactaatgat tttgctcgtg
     8641 ccttaaaaat accgcgaggt aatcctattg aagcaactaa attaattggt aaaaatcaaa
     8701 tcgttaagat ggacattggt caagctcaag aggacaatta ttttattaat attgcagctg
     8761 caggtagttt gacagagtta acttatagtg taccaagtca attgaaaaca acgtttggct
     8821 atcttgctta tctagcaaaa ggtgttgaat tattacctcg agttcgaaaa gtaccagtca
     8881 aaattactca cgataagggt gaatttatcg gtgatgcttc aatgattttt gttgccatca
     8941 caaattctgt aggagggttt gaacaaattg cacctgatgc caaattggat gatgggaaat
     9001 ttacccttat tttagtaaaa actgcaaatc ttattgagat tatgcatctc attcgtttgg
     9061 tgctagcagg tggaaaacat attaatgata aacgagtaga gtatattaaa acgtcttatc
     9121 taactatcga acccctatca gacgagcgta tgatgattaa cttagatgga gaatatggag
     9181 gagatgctcc cattactcta gcaaacctta aaaatcatat taggttcttt gctaatactg
     9241 atgaaatttc ggatgatgct ttagttttag ataaagatga attagcgata gaagctattg
     9301 cacagaagtt tgctaatgag gtaaatgatt tagaaggatt aggagattga tgaatggata
     9361 atactgtcat tgtacattac catagccgaa agaaaaatta ctttaattta agtttatggc
     9421 agtggcgcga tggctcagag ggtcaagatg ctcatttttc acggtttgac agtttcggtg
     9481 ctgttgcgat actaaaatat cccttgccat attttttaag ccattattat gtgattgtta
     9541 aggataaaaa ttggcatcat aaaacaatag attatcgcat tgattgtact aaaggtggtg
     9601 cacgtaaaga agtttggatt gtagatgggg atgacaatct gtattattct cgtcaagcag
     9661 cagttagtag ccatcattat agtagacgcc aacctcatgc ctacgatatg gcaatcaata
     9721 gtagagcgtt tgataaaaaa tggggatttg atggttggtt aggttttgaa tattcagaaa
     9781 ctaaaacggc ttttagattg tgggccccta ctgcggaacg tgtagaactg attttatatc
     9841 attcgacaga tgaaacagca agtgtatcaa aagttctttc tatgaaaaga gggacagccg
     9901 tcaattataa aaatcataaa gaaaacaccc atggcgtctg gtttactgaa cttgaaggaa
     9961 actataatta ccaagcatat acttatcgag tctactatag acgacgtacc tttaaaatta
    10021 cgagagaccc ttattccatt gcaacgactg caaatggtaa aaggtctatt gttattgctc
    10081 cagaagcatt aactccggaa gggtttaaaa taagtcatgg aaaagaagct aagtggcggc
    10141 tcgaaaaccc caatcaagca gtcatttacg aaatgcatgt gcgtgatttt tcgatatctg
    10201 agacttcagg agttaagaca gattatcaag gaaaatttaa gggattgcat caaaaagata
    10261 cagttaatca gcatggtgat aaaacgacct ttgattatgt gcaagactta ggagttaact
    10321 atatccaact tcaaccaatt ttcgatcatc atcagacgtt tgatgatgat ggtcactatg
    10381 cttataattg gggatatgat cctgaaaact acaacgttcc agaagcaagc ttctctagta
    10441 atccacatga acctgcaacg cgtattttag aattaaaatc tgctatccaa gcgtaccatg
    10501 atgcaggcat tggtgttatt atggatgttg tctataatca tactttttca tcaactgatt
    10561 ctgcatttca actaacggtt ccagattatt attatcgaat gaatcacaat ggaacatttc
    10621 aaaatggttc aggctgtggt aatgaaacag ctagtgaaaa agaaatgtgt cgtaagtata
    10681 ttcttgattc agttttgtat tgggtgaaag aatataatat cgatggtttt cgatttgatt
    10741 tgatgggact acatgatgta gaaacgatga acattataag aaatgagcta aataaaatcg
    10801 accctcgtat tttggtttat ggagaaggat gggacatggg agctggtctc acgcctcaaa
    10861 ataaagctaa aaaagataat gcttatcaga tgcctggaat tggctttttc aatgatgatg
    10921 taagagacgc tgtcaaaggt gcggaaattt acggagaatt taaaaaaggc ttagtttctg
    10981 gaaacagtac agaagatatt gttgcaaaag gcattttagg aagtgatgag ttagtatcat
    11041 acattgatcc tagtcaagtt ttaaattatg ttgaagcaca tgacaactac aacttaaatg
    11101 atttgctatg ggaattacat cctaatgaca acgagaaaca acatatttac cgagttgaag
    11161 tagctagtgc tatgaatcta ttgatgcaag ggatggcttt catgcaatta ggtcaggagt
    11221 ttcttcgaac aaagtgttat cctacaggtg ataaaggtca acttactcaa gcagataaag
    11281 aaagagctat gaattcttac aatgccccag accaagttaa tcaggtcaac tgggataatg
    11341 ttacctttca taagagtact attaatttta ttaggaaaat aattactttg aagacaaact
    11401 ctccttactt ttcttactct agctttgaag agattcgcaa acatgttttt gtagagtctg
    11461 ctcaatacca ttcaggtttc ataagtttta cagtcgaaga acataagaaa ataaaaataa
    11521 ttttcaatat taaaggaaaa cgcttgcagg atcctttcat aggtgatata ataatatcaa
    11581 atcatgaaga aggaaaagcg atagattcat tagatgatga gctttctgta atgatccttg
    11641 atatcacaaa ataacctgaa aacattcagg ttatttttgt gtatattaag gaatttgatt
    11701 tgaatttgag tttcttatat tttagttaat ttttcggaaa tatagagggt gaaatatttt
    11761 cagatgcgtc tgtatgagga aaatgcattc ctcttttttt gtaccattga ggaaatattt
    11821 ttgtcttata ttgactagta gacctgtgag gtgagtagat gtccaataaa gatgagttat
    11881 atacttttgg aataggtgaa aattttcatc tccagaatta tttaggcgta catagcgaaa
    11941 atggaagctt ttgtttcaga gtttgggctc ctaatgctga aaatgttcaa gttattggag
    12001 attttacaga ttggcgaaat cgacctctcc aaatgaacaa aattcaagca ggagtttggg
    12061 aagctaacag tttggatgcg cgcgaaggtg atttatataa atatttggtt actcgtaaag
    12121 gtggtcaagt agttgagaaa atcgatccta tggcagttta tatggaaaga cgtcctggaa
    12181 ctgcaagtgt tattaaagtt cttagaaata aaaagtggga agatggtctt tggatgggaa
    12241 ggcgcaaacg tctgggtttt cagaagcgtc caattaatat ttacgaagtt catgctggtt
    12301 catggaaaaa agatgatttt ggacatccta tgactttttc tcagttaaaa gattacttaa
    12361 tcccctacct tgttgagatg aattatactc atgttgaatt tatgccctta atggcacatc
    12421 cgcttgatat gagttgggga tatcaattaa tggggtattt tgcttttgaa catacctatg
    12481 gtactccaga agaatttcaa gactttgtag aagcatgtca taaaaataat ataggagttc
    12541 ttgtagattg ggttccagga cactttattc agaatgacga tgctttagcc tatttcgatg
    12601 gaactgctac ttatgagtat caaaatcatg atcgtgcgca taactatcga tggggagctc
    12661 tgaactttga tttaggaaaa aatcaagtac aatccttttt gatttcgagt gccttgttct
    12721 ggattgaaca ttatcatatt gatggtattc gtgttgatgc ggttagtaat atgttatatc
    12781 tcgattatga tgaaggacca tgggaagcta accaatttgg ggataatcgt aatttggaag
    12841 gatatcactt tttaaggaaa cttaacaaag taattaaaga gcgtcatcct aatgttatga
    12901 tgattgctga agagtcaaca gcttctaccc caataactaa agacttagag tctggaggat
    12961 tgggttttga ctttaaatgg aatatgggct ggatgaatga tatcctgcgt ttttatgaag
    13021 aagatcctct ctataggcaa tatgatttta atttagtgac ttttagtttt atgtatatat
    13081 tcaatgagaa ctttgtacta gctttttcac atgatgaagt agtacatggt aaaaagagta
    13141 tgatgcataa aatgtggggt gaccgttata atcaatttgc tggtttacgt aatttatatg
    13201 catatcaaat gtgtcatcct gggaaaaaac ttctctttat gggaagtgag tttggtcaat
    13261 ttttagaatg gaaatataat gatcaactag aatgggaaaa cttaaatgat gatatgaacc
    13321 aaaagatgca acgctatact aaacaattga atcaatttta taaagatcat aagtgcctat
    13381 ggcgtattga tgattccttt gacgggctag aaattattga tgcggataat aaatctgaaa
    13441 ctgttttatc ttttatacga aaagatgata aaggcgattt gttactttgt gtctttaata
    13501 tgactcctgt agaacgccct aactttacta ttggtgttcc gcaagcgggt atttatgaag
    13561 aagttttaaa tactgaaatg gaagagtttg gaggtgtttg gaaaaaccac aatcctgtaa
    13621 cgaaaacaca agtggctaca tggaaagatt atgatcatac tttaagtttt acattaccag
    13681 cccttggggc aagtgtttgg agaataaaac gtcggcttcg taaataagtc tgcaatttag
    13741 taacctcatt ttgaaaggaa agaataatat gaaaaatgaa atgttagcac tcattttcgt
    13801 cggaggacaa ggcactcgac tcggaaaatt gactcagagc attgcgaaac ctgctgttca
    13861 atttggaggt cgttaccgta ttattgactt tgcgctttca aattgtgcga attcaggtat
    13921 taataatgtt ggagttatta ctcagtatca accgctggaa ctcaatactc atattggaaa
    13981 cggatctagc tggggactag atggtatcga ttcgggagta actgtattac agccatattc
    14041 tgcaacagaa ggtaatcgct ggtttcaagg taccagccac gctatctatc aaaatattga
    14101 ctacattgat cgtattaatc cagaatatgt actcattctc tctggggacc acatctataa
    14161 aatggactat gatgacatgc tacagacgca taaagataat ttagcaagcc taacagtcgc
    14221 tgttttagat gttcctttaa aagaagcaag ccgttttgga attatgaata ctgattcaaa
    14281 cgaccgtatt gtcgaatttg aagaaaaacc tgaacatcct aagtcaacaa aagcttcaat
    14341 gggaatatac atttttgact ggaagagatt acggacagtt ttaattgatg gtgaaaagaa
    14401 cggcatagac atgtcagact ttggtaaaaa tgttatacca gcatactttg aatcgggtga
    14461 acgtgtttat acctataatt ttgatgggta ttggaaagat gttggtacta ttgaatccct
    14521 ttgggaagct aatatggaat atattggaga agataacaaa ttacatagtc gtgatcgttc
    14581 atggaaaatc tattctaaaa atttgattgc accacctaat ttcatgacag aagatgcaaa
    14641 tgttaaagat tctttagttg tagatggatg ctttgttgca ggaaatgttg agcactccat
    14701 tttatcaact aatgtccagg taaaaccaaa tgctattatt aaagactcct ttgttatgag
    14761 tggcgcgact atcggtgaag gtgcaaagat aaatcgagct attatcggtg aggatgctgt
    14821 tattggtgat ggtgttgtga ttgatggaag taaagaagta gaagttattg gttataaaga
    14881 agtagcgggg gttccaaatg aagattgata aatatactgc gattttagga aatactgtta
    14941 gttacccaga tatgcacagt ttgacggata atcgtccgct tgcttctcta ccttttgatg
    15001 gtaagtatcg tttaattgat tttcaattat cctctttagc taatgcaggc gttagaagta
    15061 tttatggtat ttttcgtggg aataatgttc gctctgtttt tgaccatatt agaagtggtc
    15121 gtgagtgggg cttaggtaca ctgttgagtc actattcttt aggattctat gatacagacg
    15181 atgatactcg tacagtcaat ggggattatt acgattaaat tttaacttac ttgaggcgtt
    15241 cgggtagcga ccagacgata tatatgagtt gtgatatgct ttgtaatatt gacttaagtc
    15301 aagtaatcca cttgcataat gcaaatggtg tgcaagccac cgtggtttat aaaaaaatgc
    15361 cgagatcagc tattgcagag gctaatcaag tcttgaccat tgatgaatct gatcatgtag
    15421 tgagtctagg taaagctgta gataatgacg ataaccaaaa aatgtctgct gagatttatg
    15481 ttatcgatac accttggtta atcgaaaaaa tggaagagga agctcaaaat aatgaaccac
    15541 gtaaactccg cttcttactt cgtgatttaa ttgttgaaag taatgctttg gcttttgaat
    15601 acactggtta tttgtcaaac atttcttcta ttaaatccta ttatgatgct aacatggaca
    15661 tgttaacacc aaataaattt tattcgctct tcttttcaaa ccaaaaagtt tatactaagg
    15721 tgaaaaatga ggaagctact tattttgata aacaatcaaa tgtctctaat tcgcaactag
    15781 cctatggaag tattattaaa ggttatttag accattctat cgtttctaga aattgtctcc
    15841 ttgaaaaagg gactcgagta gtcaatagta ttatctttcc aaaagtaaaa atcggagaag
    15901 gggcaaccat tgaaaatact attattgaca aatgcgttaa ggttgcatcc ggagttacat
    15961 tgaaaggtag tttagataaa ccgttagtga ttcctaaatt ttcagagatt aatgaggata
    16021 ttatacaatg aaaataatgt ttgttgcggc agagggtgct ccctttgcta aaacaggtgg
    16081 tttgggagat gtgattggcg cactcccaaa atcacttagt aaaaaaggtc atgatgtcgc
    16141 tgttgtcatg ccatattatg atatggtaga ccaaaaattt ggagaccaaa tagaaaacct
    16201 catgtatttc tatacagatg ttggctggcg tcatcaatat gtaggagtta agagactttc
    16261 tcaagataat gttacgttct actttattga taatcaatat tatttttata gaggtcatgt
    16321 ctatggtgat tgggatgatg gggagcgttt tgcttatttc caattagctg ctttagagtt
    16381 gatggagaaa atagatttta ttcccgatgt cttacacgtc catgattatc atacagcaat
    16441 gattcctttc ttattgaaag agaagtacca ctggattcag gcctataata atataagagc
    16501 agtttttact attcacaata ttgaatttca agggcagttt ggtccagaaa tgttaggaga
    16561 tttatttgga gttggcgctg agagatatga agatggaact cttagatgga ataattgtct
    16621 taattggatg aaagcagcaa ttctttactc agatagagtg acaacggtat caccaagcta
    16681 cgcaaatgaa ataaaaactc cagaatttgg taaaggttta gaccaaatta tgcgtatgga
    16741 agcaggtaaa ttatctggaa tcgtaaatgg tattgacagc gatttgttaa atccagaaac
    16801 agatgctttt ttgccatatc atttttcaaa aagcaactta gagggcaaaa ttaaaaataa
    16861 gttggcttta caggaaaatt tgggcttgcc tcaagataaa aatgtaccat taattggtat
    16921 tgtttcacga cttaccgatc aaaaaggatt tgatattatt gcttctgaat tggacaatat
    16981 gcttcaacaa gatattcaaa tggttatttt aggaacaggc tatcaccatt ttgaagaaac
    17041 gttctcttat tttgctagtc gttaccctga aaaactatct gctaatatta cttttgactt
    17101 aggattagcg caacagattt atgcggcttc agatatcttc atgatgccaa gtgcttttga
    17161 accttgtggt ctctcgcaga tgatggctat gagatatggt agcttaccct tggtacatga
    17221 agttggagga ttaaaagata ctgtggtagc cttcaatcaa tttgatggta gtggtacagg
    17281 tttttcattt aatcattttt caggctattg gttaatgcaa actctaaaac tagcattaga
    17341 agtttataat gattatcctg aagcgtggaa aaagttacaa tggcaagcta tgtcaaaaga
    17401 tttttcatgg gatacagcat gtgttgctta cgaacaactg tatcagcaac tacaataatc
    17461 actttatttt agtgattagt tgataagctt atcgaatctc tatgctaaac gtttcttatg
    17521 taaaaccact ttagtataga gattttatta tcttataagg taaaatatta ttaatgtaaa
    17581 gagacactat atcaaaaata aagtgttgtc atatcttaat aagggattaa attaaagcta
    17641 aaaaaactaa aaattgttat ttgaaaagga ttgtgtaaac catctcaaac tatttcatga
    17701 aaacgtaatc aaatgaggaa attcattgac atgcacaaga aataggaata aaataacgga
    17761 gtaaatattt tttaaaggag aattctatta tgaatttagc tattttagca cttggtttcg
    17821 ccgtgatggg tgtttcaatc ggtgaaggaa ttttggttgc aaatattgcc aaatcagcgg
    17881 ctcgtcagcc tgaaatgttt agcaaactgc aaacattgat gtttacaggt gtggccttca
    17941 ttgaaggtac cttcttcgtt ctatttgcgt ttacattctt agtaagatag acgacaattt
    18001 agaaaaaagg agggatgagg attggaatct acaagtaatc caaccgtatc ttttttaggc
    18061 atcgattttg atttaaccat cctagctatg tctctcttga caattactat tatctttatc
    18121 ttagtcttct gggctagtcg taagatgaca ataaaaccaa aaggtaagca gaatgtctta
    18181 gaatatgtct atgagcttgt taataataca atctcacaaa atttaggaca ttatactaaa
    18241 aattatagtt tattg
//
LOCUS       DATRME010000037        17650 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRME010000037 DATRME010000000
VERSION     DATRME010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17650
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            16..996
                     /locus_tag="VBC23_001229"
     CDS             16..996
                     /locus_tag="VBC23_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6843178.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(1081..2829)
                     /gene="cydC"
                     /locus_tag="VBC23_001230"
     CDS             complement(1081..2829)
                     /gene="cydC"
                     /locus_tag="VBC23_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6843179.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVTLHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(2822..4540)
                     /gene="cydD"
                     /locus_tag="VBC23_001231"
     CDS             complement(2822..4540)
                     /gene="cydD"
                     /locus_tag="VBC23_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6843180.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(4540..5559)
                     /gene="cydB"
                     /locus_tag="VBC23_001232"
     CDS             complement(4540..5559)
                     /gene="cydB"
                     /locus_tag="VBC23_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6843181.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(5560..6987)
                     /locus_tag="VBC23_001233"
     CDS             complement(5560..6987)
                     /locus_tag="VBC23_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6843182.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(7090..8298)
                     /locus_tag="VBC23_001234"
     CDS             complement(7090..8298)
                     /locus_tag="VBC23_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6843183.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(8311..9210)
                     /locus_tag="VBC23_001235"
     CDS             complement(8311..9210)
                     /locus_tag="VBC23_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6843184.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(9619..10224)
                     /locus_tag="VBC23_001236"
     CDS             complement(9619..10224)
                     /locus_tag="VBC23_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843185.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            11107..11406
                     /locus_tag="VBC23_001237"
     CDS             11107..11406
                     /locus_tag="VBC23_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6843186.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            11566..12756
                     /locus_tag="VBC23_001238"
     CDS             11566..12756
                     /locus_tag="VBC23_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843187.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(12826..13551)
                     /locus_tag="VBC23_001239"
                     /pseudo
     CDS             complement(12826..13551)
                     /locus_tag="VBC23_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(13678..14265)
                     /locus_tag="VBC23_001240"
     CDS             complement(13678..14265)
                     /locus_tag="VBC23_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6843188.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(14309..14845)
                     /locus_tag="VBC23_001241"
     CDS             complement(14309..14845)
                     /locus_tag="VBC23_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6843189.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            14980..16152
                     /locus_tag="VBC23_001242"
     CDS             14980..16152
                     /locus_tag="VBC23_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6843190.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(16196..16978)
                     /locus_tag="VBC23_001243"
     CDS             complement(16196..16978)
                     /locus_tag="VBC23_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6843191.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            17137..17406
                     /gene="rpsN"
                     /locus_tag="VBC23_001244"
     CDS             17137..17406
                     /gene="rpsN"
                     /locus_tag="VBC23_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6843192.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT         5972 a         3406 c         2760 g         5512 t
ORIGIN      
        1 gagggagact gattaatgtc atcttattgg aataactatc ctgaacttaa aaaaaatatt
       61 gatgaaacca atcaactaat tcaagaaaga atacaggtca gaaataaaga tattgaagcg
      121 gcgctaagcc aactcacagc tgcgggagga aaacagctca gaccagcatt cttttacctt
      181 ttttctcaac ttggtaataa ggagaatcaa gatactcagc aactaaagaa aatcgctgct
      241 tctttagaaa tccttcacgt tgctacatta atccatgatg atgtcattga tgactcacca
      301 ctaagacgtg gaaatatgac cattcaaagc aagtttggca aagacatcgc agtttacact
      361 ggggatttac ttttcacagt ctttttcgat cttattttag aatctatggc tgatacacca
      421 tttatgagga ttaatgcaaa atctatgcgt aaaattctca tgggagaatt ggaccagatg
      481 caccttcgtt acaatcaaca acaaggtatc catcactatt tacgtgcgat ttcaggtaag
      541 acagccgaac tctttaaatt agctagcaaa gaaggagctt actttggtgg tgcagagaag
      601 gaggttgttc gtctagcagg ccatatcgga tttaacattg gtatgacatt ccaaattttg
      661 gatgatatcc tggattatac tgcagataaa aaaacattta acaagcctgt cttagaggat
      721 ttagcacaag gcatttacag ccttcctcta cttcttgcca ttgaagaaaa tcctgatatt
      781 ttcaaaccta ttttagataa aaaaacagat atggctactg aagacatgga aaaaattgct
      841 tatctcgtcg tttcccatag aggtgttgac aaagctcgcc atctagctcg taaatttact
      901 gaaaaagcta ttagtgacat aaataagcta ccccagagct ctgcaaaaaa acagttgcta
      961 caattaacta attacctttt aaaacgcaaa atttaaataa taaaaaaaca ttccacaatg
     1021 ctagaaaagc agttagggaa tgttttttta ttatcattta tttatcgcac ctatcaatca
     1081 tcatagatca ccatcatcag cggctttcag ctgacggtaa cgttggctac tttgagacaa
     1141 ttcttgagga gaaccttcca actctaattg cccattttct ataaataaga tacgatcagc
     1201 atgttcaata ccttttaagt gatgtgtaat ccaaactaag gtcttacctt ccaattcttt
     1261 cataaatacc cttagtaagg cttgttcagt aataggatca agtccaacag ttggctcatc
     1321 taagataaca attgggacat cttttagtaa gattctagcc aaagcaattc tatgcctttc
     1381 gccacctgaa aacctaagtc cagcttcatc aaccattgta tagagaccat ctgataaatc
     1441 agtgaccatc tctttcaatc caactcgttc aagaactttc catacatctt cttcactagc
     1501 atcttggttt ccaatgcgaa tgttatttag cagggttgta ttaaaaaggt agggcgcttg
     1561 ttgtatcact ccaatatagt tagaaatgca atcaccaact attgaaacat cagcaccgcc
     1621 tagggtaatc tccccttgac ttgctttcaa gtcgccacga agtagactgg ctaaggtact
     1681 cttgccagaa ccactacgcc ctaaaatagc aattttttct ccttctttaa tatccaaatc
     1741 taaatgatgc aaaacccatt tctcttgtgg cttatactgg aaacttaaat tcttgacgga
     1801 aaaatcgtat ggcttattag gcaactgatt ttgagttgtt tcaaaatacg tttctgataa
     1861 ttcattaaga cgatgaattg aatcagaata tttatttgtt tcttgagcag cagcagacaa
     1921 accagcaaat gcctctgaca agggaaatac tgttagaaca aatgctgcaa tccagttagc
     1981 ttcgccacct ctgtgaccaa taaactgatt actagcccaa ataataacca aaatagctag
     2041 aaagccaaaa actaattcca caataagagc tctacgatta tcaaaggaac gaattttttt
     2101 ctgaacagcc atgagttcac tttccgaacg ctcatgcaag gtgacgtact cttgaccacg
     2161 ttgactaaaa atccagtctg aaatacctaa gacattatcc gtcaaatcag tataaagatg
     2221 attttttaat tctttttcac gagtctgacg agcaccatta gctaaaatcg accaaagtgg
     2281 aaaaagaaaa atcataatag ctaaataaag caacatcatt aaagcaaacc atagagaaaa
     2341 gaagcctgtt gcaataataa taaagctata taatatccac gctataatcg ttggaaatat
     2401 tgtcctaaga tacaaatttt gcaaataatt aatatcctcc gctaaaagcc ccataacatc
     2461 tcctaaacga aaatcacgct tcataaagat agcattgctt tctaaggaat ggtaaagctt
     2521 caagcgcagc tgtgaagtca ttcgtaatac ccaattgtga ctcgttaggc gttctatata
     2581 acgaaaaaca ggacgtccaa tgccaaaagc acgtgttagt acaattggaa catatactag
     2641 aagaatatta ctcggtagtg aagctgactt actaatcaga tagcccgaat taaacataag
     2701 agcactggca gaaaagaaag tcataaaacc aaggaaaaga gcaattacta aggaaacttt
     2761 atattgtttg aaaaatggtt ttatccattg atctgtcttt aattctttaa acaaagggat
     2821 tttaaacatc atttcctccc atagcatgct tcagttgata gagatatcct tttttagcta
     2881 ataattcctg ataagagcct acttctgcaa ctctcccctt ctccataacg actataacat
     2941 ccatttggtt caaccagtga agacgatgag ttgcaaagat aactaaacga tctgacatca
     3001 gtggcaacat tttctctttt aattctaact ctgtttcaat atctaaatga gcagttggtt
     3061 catcaaaaac cataatacga cgattttgat caagaaaagc acgagccaga gctatacgct
     3121 gggcttgtcc tccactaagc ggacgcgctc cattaccaat acgcgtttct aaaccgtcag
     3181 gtaactcgct tagcaagcta tctaaaccta ccatatgaat cgctcttacc acctcttcgt
     3241 cactagcatt gggagtatag aaggtaatat tatcacgtaa tgacatttca aaaacataag
     3301 gagtttgagg aatatagatc atctgtttat gccaatcttc ttgatcaagg ttagtcactt
     3361 ctctaccatc cacttttaaa gaaccattat caggccctaa aaaaccactc agaaggttaa
     3421 ttaagctaga cttacctgaa ccactaactc ctactaaagc aaccttttga tgtcctctaa
     3481 atcgtaaggt tacatcctca acaacacgtt tttggtcgta cgcaatagca atgttttcta
     3541 aatccaaacg actctcttta gaccaatcat ctatgactaa ttgctcacct ttaataccag
     3601 ttttgtttag aactttttga atcgcttgaa atgcattttt cccgtctaac gttgcatgat
     3661 aatcactaga aaaatctctt acgggtaaaa agtattcagg ggataaaatc aaaattgtta
     3721 aagccggtaa aaggtaaatc tgctcattta ataatcgtaa acctagaaaa acagcaacga
     3781 tagcaatgga taaagttgta aaaaagtcta aagcaaacgt tgataaaata ccaattttca
     3841 aagtactcat tgttgcttta cgaaaagatt cactagtttg ataaatgctt ttaccataac
     3901 gtttactaag accaaaatat ttgagagtat caatgcctcg taatgaatct aaaaaatgat
     3961 tagaaagtac ttgataggat tcatattgtt tatctgcttt agcttgagcc gcatagccta
     4021 aaataatcat aaagatgata attaaaggaa aaacgataag taaaattgca cctgattcaa
     4081 tatctaaata gaaaatataa gctagaataa tccatgggat aatagacatg ttaatcatct
     4141 tattcaagac taaacgtaaa taattttcaa ctaaagaaac gccatctaat gccattgtaa
     4201 caacattacc agttccttgt tcttgaacga ctttaggacc tagatcaaat aacttatcta
     4261 ataattgacg tctcaaaaga ctagattgtg ctgttgaaaa gtcatcaagt tttctatttt
     4321 tgatgtaatc aataacatga cggcctaaat atgagaccat aaataacaaa acataaaccg
     4381 tttgagaaga aagtttttgc ccttcccaca aaccagttat agaaagactc aaataataag
     4441 cttgtccgat aataaagatt gcttgaagaa catcaagtcc cgcaagcaat cctaacaact
     4501 tatgaatacc tgacaagcgc ataaccgctt tatctagcat tatttttctc ctgtcacaat
     4561 cactggcaag gtaatacgtt ttctaaagat ataatatgcc catgctgtat aagctagaac
     4621 aaagggaact agagtaattg cgacaatcga cattactttt aaagtatatg gcgttgagga
     4681 tgcattttga atcagcaaat catatttagg gctaattgat gaaatcataa ctcgtgggaa
     4741 tagaccttgg aacaacagaa caaccactga aacaagactg agaccactcg ctaaaaatgc
     4801 tgtcatctct gctcctttaa aagttgaagc atgcgctaat acagctaata caacaataac
     4861 caaaaccata attgtcgtaa ctatcggatg attgctaaag aaatctgtct tgaaatacaa
     4921 gagaactgca aacaaggcta aaccaagata gagtacccaa taaagcaact gagcataatt
     4981 acgggcacgc tctctaattg gcccttctgt cttcaatgca atgtaattta aaccatgaag
     5041 ataggctagc aataccattg caactccacc caccaacgag aatatattaa agtaatcact
     5101 gaattgagct gataagttac cactggcatc aagtggcata ccttgaatca aacttataaa
     5161 cataatccca aagaagaatg gaacaatagc ggaaccaatt gttaaggtcc agttccaaaa
     5221 ctgctttttc tctgcaggaa ctttatgtct aaattcgaag gaaacaccac gaataattaa
     5281 tccaaagaga atcgtaagga gaatcaaata gtaaccacta aatagtgaag cataccagta
     5341 tggaaaagaa gcaaacattg ctccaccacc ggtaagaagc caaacttcat taccatccca
     5401 aactgggcca atggtttcta ctacttgatc tttttcatgc tcattatgtg ttaaagtttg
     5461 aacagccatc ccaaccccaa agtcgaatcc ttcaaggaag aagaatcctg aaaataggag
     5521 gccgattagg aaaaaccaaa agaattgtaa agcactcatt tagtatgctc ctttctcaaa
     5581 gggatcaatt gaagtatatg caccatgatg tccgtgttca tcttcatact ctggcccttt
     5641 cttcaattct cttattgtta ggcttatcat catcaccgct aaaccaccaa acaacaagaa
     5701 atacacagta ttggaaacaa ataatgatgc aactgaaacg tttggagaga cagagtcctt
     5761 aatcttgaaa agaccataga cagtccaagg gtatcgtcct tgttctgtca caatccaacc
     5821 aaatgtattg gctaagaatg gtgcaaaggt tgttaaagca acaatccaaa gcatccattt
     5881 gtgtttataa agaattggtt ttttcttacg agttaaaaag agtcctaaaa cagaaacacc
     5941 cataatagct gttcccatcg ccgccattgt acggaaacca tagaaaagta ggttaaccat
     6001 tggatagtag ttatctttac catacttagc aaccaattct ttattagctg tatccatacc
     6061 tttcacagaa ccagatggtt tcccgtaaga taaaatactc aacatataag gaatcttaat
     6121 cccaaaaact tgtttgtgtt ctgcttcatt tgcccaagca actacactcc aagctgcagg
     6181 atcacccgaa tcttcataat caccttccat agcagcaaat ttcattggtt gatcgtgaat
     6241 caaggctttc atttgtaaat ccccaactcc cataactgaa attgaaccaa gaagtgctac
     6301 tactaaacca atacgaaccg atttcttata aagttcaacc gttgtatctt tcagctgttc
     6361 tgattttaag aggcggaagg ctgacatacc agcaaccact gtaccaccca tcgtaatggc
     6421 accaaaaata acatgggtaa attcatagaa gaactgtgga ttttttacaa gtgcaagaaa
     6481 atcagtcatt tgcgctctac cgtttacaac ttcataacca acaggatgtt gcatgaagct
     6541 attagcagta agaatccaca tcgctgacat taatgagcca aaaaccacta gccaaatgaa
     6601 tgtcacatgt aattttttac taattttttt gttatcccat gtaaacatcc ataaaccaag
     6661 gaaagtagat tccataaaga aagctaataa agcttcaata gccaatggag ctccaaaaat
     6721 atcacctacg aaacgtgaat agtctgacca gttcattcca aattggaact cttgaatgat
     6781 acctgtcaca acaccaaccg caaaactaag gagcatgata ttaccccaaa attttgtcaa
     6841 ttttttatac tcttcatttt ttgtgataac atacattgtc tccataatag caaccactag
     6901 gcatgtacca attgtaaatg gtacaaagaa aaagtggaaa actgtagtca tcgcaaattg
     6961 aaaacgagct aaagtttcaa ttgtcataca aaacctctta ttttgtttta atcatttctt
     7021 ttatagaaat atctgcaaga gctagtttcc tagcttcgta acaattggta aataacttac
     7081 tttaatatct taatgatata aatcaaaacg tcccttagct agaagttctt taagtccacc
     7141 catatcaaca agtgatttgt tcatgataat cttcttcata accgacgcag ggtatttttt
     7201 aattttagtt tttccaacaa caccaagtcc atgcgtattt cctactgaag caactgtgcc
     7261 ttgcggacta tacgaaaagt cttctgtagc ttcacctttg atttgatgta aaaggttttt
     7321 agcaacatga gctcccatac gagttgcaat ttgagctgtt gttgggaatg ggcgaccaga
     7381 ctcagtgtcc ataaatgcag acacatcacc gataacataa acattatcgt attttggatc
     7441 acgcaaatca ctgttgacca ttacacgtcc acgacgttga tcaaatcctg attctcccat
     7501 aaccggactt ccactaacac ctgttgtcca aatgatagta cctgcggtaa tagatttgag
     7561 ttcttcatcc tctttactag taccataaac aacttcaccc ggtttgattt ccttaatcat
     7621 acttccaagc atcaaattaa tacctaaatc cttaattaag ttgacaccat attgcgctaa
     7681 ttcatcatca aacattggta ggatgcgagt tgctgcctct acacaaataa tttcaatttg
     7741 ttcaggtttt acacccgcta aagcagcata ttttggtcgc tcatcaacca tagctccggc
     7801 taactcaata cctgtaaaac cagcaccaca tactaagagt ttgagaagat tttcatcttt
     7861 tgtctcacgg tacaacttca ttgtattaac aatgtgatta tggatgtttt ctgcttgcga
     7921 aatattggtc atctgcaaag cattctccat agcacccttg atgccaaaag tttcagatac
     7981 aaagcctaag gcaactacga cataatcata atggagttca ccgttatttt taactgttac
     8041 tgttttattt tcagcatcga ctctaagcac ctcatcttgc atgaagttaa ccttattagt
     8101 gttgataaca tccttaattg ggaatgtaat tttttctttt ggctgagagc ctgcagctac
     8161 ttcatgtaat tctgtagctt cgtagtgata atcattcatg tcaacaaggg taatatgaaa
     8221 atcacctgat tgtttttgta aattacgaac tgctttcaaa ccggcataac cagcacctaa
     8281 aactaggatt tctttcatga taaaactcct ctaaaaaatg attccaatta gatacgttaa
     8341 gacttgggtt gcagatccca gtgctagaat acgtaccgca caaacaaatg tttctctctt
     8401 aacctgtttt tgccacaata gttttgtctg cttataaata aatggtatta ataacaaact
     8461 caagagtact gtaacaggcg caagtccaaa aagaaattca aaaacaattg ccaataaagc
     8521 tatactatta gaaatagcaa acagcaagag tccacctctt atccctgtat aatgtacaag
     8581 ggtatagcga tgattttttt catcttcttc tttgtcgcac agattattcg ccaacattag
     8641 gttggcaatc cataaggtgt tcggcaagga aatcaagaaa attttgctta agttgaggat
     8701 atcccacgaa aagttttgat aagtgtttag atatacacaa attaaactaa tcataaatcc
     8761 cattgtcaag ccagaaaata cttctcctaa gggcagacta gataaaggtc ttggaccaaa
     8821 ggaatataaa acaccaattc caaaacaaaa aagtcccatc cataaaagag gcagaccgac
     8881 ttgggaaact aaaactaaac caatcatact agaagtaatg actaaacttg ccataataac
     8941 ttctatttgg cgtagggata ggttctcacg accaataatg ttagtgtcat tcttataatc
     9001 caaatccaca gcattccgat aatcattgta attattccaa atgtcaacga acatattgaa
     9061 taaaaacata gcaacgaaga ataaaccgac atatacaggg tgtactgaat tgtagtaata
     9121 agcactaaaa cacaaaccaa tcaaaaatgg taaaacacta gctgttttgg ctttcatttc
     9181 taccaactct agaaagattg gtaatgtcat tattaaagtc ctctctatac ataatcttca
     9241 acaatactat tataatgcaa tttctaccat ttttaaaaca atagggcttg atttttgatt
     9301 aaaaatacaa aaaatttttc ttaaaatgag ttaatcacac ttagaacact aaatactatt
     9361 ttgaggagtc ctattcatca cactatgcca tgtaatattc ggaaaaatag tattgcaacc
     9421 ctcaactatc ccttatgttg gcaccagcct ttgaaagtcc tctgattgca ctgcttagtt
     9481 agtattttca tcagctgtat gatctctaag agccatcagc atactaaaaa aactagtatt
     9541 acactttgaa aagtgtaaca ctagctccaa tttgttttaa atagcttaac tatttaatca
     9601 ttaatctact ttatatattt aagcatttaa agttgaatgt tggttgagga tacctgagag
     9661 agaaccttga atataatttg gcaatacttg tttttgagca cgttcataga tttctgaatc
     9721 tgttaactga cgttctgatt tggggcttga tacttgcggt gtcactgctg cattttgttg
     9781 tttgtaaagt gttgaatgct ggttaaggat acctgagaga gaaccttgga tatattttgg
     9841 taatacttgt ttttgggcac tttcataaag ttctgaatct gttaactgac gttctgattt
     9901 ggggcttgat acttgcggtg tcactgctgc attttgttgt ttgtaaagtg ttgaatgctg
     9961 gttaaggata cctgagagag aaccttggat atatcttggc aatacttgtt tttgggcacg
    10021 ttcataaagt tctgaatctg ttaactgacg ttctgctttt ggaggtgttg cttgcggtgc
    10081 tgtgaaagtt gaagctgaaa caccttgtgt agaaccaaga gctacgaatg ctcctccagc
    10141 aaacattgct gagacaagac caagtttcaa atagttagca gttgagacgt tgttgacttt
    10201 tttttctgta ataagtttgt tcataagaat tacctctttc ttttgggtta tttattttat
    10261 gaactaagta taacaacaag aaataaagta aggttaaact aaatataaaa taattcttaa
    10321 gtcattctta ggaaaccata aaatcataca ctaacaaaaa atttggtatc tagctcaaaa
    10381 gtaaaagtag aataaaggct tttacttctg agctagtaat gaataaatta tatttgcatc
    10441 tagtttggaa ttagccctag tgtattataa tgatccctac aaatatgtgc caaacacctt
    10501 cttcatatat acaagattga tttaacattc ctataccctt aatggtgggc acattctctt
    10561 catgttccat atagaaatca acagataatt ccgtattgtc cctattatta aattttccag
    10621 ttaatgataa attatatcgt ccaccttgag gtgtcctatt accaatctgt tcaaccttta
    10681 cacttgacga atcgaattga acagattctg tcttatcatt ctgtgattta gaacatcaac
    10741 aatttcttgt tcatgttctt tgaggtaagc tagttgttct tctttaacgt gcttttatct
    10801 ttcgtactta ctgatacttg agagcatttt tttgacccta cttcttacta atcagctgac
    10861 gtccctataa gatgaatcat aaacacatag ctgccactaa ccattttaac cttttatgca
    10921 tacataattc ttcttgatat cccataccat tatctttagt ataaacactt aataaaacaa
    10981 gcatttaaca actaaggtta ataaaattca ataaatctaa tccttctact tactttagtt
    11041 tatcactttt ttctctcatc ctatgctata atagtaaggt atcaaaattt aggagaatat
    11101 agtcttatga tgaatatgca aaatatgatg cgtcaagcac aaaagcttca aaaacaaatg
    11161 gaacaaacgc aagctgattt agcagctagc cagtttactg gaaaatctgc tcaagagctc
    11221 gttaccgtga catttacagg cgataaaaag ctaatttcaa ttgattacaa agaagctgtg
    11281 gtagaccctg aagatattga aacactacaa gacatgacaa ctcaagctat caatgacgct
    11341 ttaagccaag ttgacgatgc tacaaaaaaa attatgggag catttgcggg taaaatgcca
    11401 ttctaaaaaa taaatcacat ttactgaatc acctgtaaat gtgattttta tttcaaactt
    11461 ctttcatttt ttcacgaaaa caagttttat tttcaaaaaa tatttgcaaa aaatcagcag
    11521 agaggtataa taacattgaa aatattagag agaaggtgac taaccatggt tatggatagt
    11581 ctaatcataa aacagttgat taagagtaca tttgatatac ccttacaagt cacttatcca
    11641 aatgggaata ttgaaactta taatggtagc aatccccacg tcaaacttaa actaaacaaa
    11701 aatttctccg tctcggagct atcaaaagac ccttccatag tattgggtga agctgttatg
    11761 gatggagaca ttgaaattta tggttctata caagaattaa ttttatcagc ttaccgctgt
    11821 ggagacagct ttctacgtaa tagtaaattt agcaaattga ttcctaaaca atttcatgat
    11881 aaaaaacaca gtaaatccga cattcaaaag cattatgaca tcggaaatga tttctataaa
    11941 ctgtggcttg atgataccat gacttattca tgcgcctatt ttaaacatga aaatgatagt
    12001 ctagagcaag ctcaactaaa caaagtacac catattctta ataaattaaa tgctcagccg
    12061 ggagggaaat tactagacat cggttgtggc tgggggacac ttatcattac tgctgctaaa
    12121 gaatatggct taaatgcgac aggtatcacc ttaagtgaag agcaggcttc tttcattact
    12181 aagcgtatca aggaagaagg gttggaaaat aaagtaacag tcctcattaa agattatcgt
    12241 gacattcgtg aaacatacga ttatatcact agtgttggta tgtttgaaca tgtaggaaaa
    12301 gaaaatttat ctcaatactt ccaaaccatt tctaaaagac tcaacatcaa tggtctagct
    12361 cttattcatg gtattactgg acaagtgggt ggtaatcatg gtagtggaac taattcttgg
    12421 attaataagt atatcttccc aggaggatat atccctcgtt taactgaaaa tttaaatcat
    12481 atagctagtg ctggacttca aattgctgac ttagaaccgc ttcgtcgtca ctatcaaaag
    12541 actctcgaat tatggacaaa aaatttccac aatgcccttc ctgaagttca gaaaactcat
    12601 gataagcgtt ttatcaatat gtgggacctc tatcttcaat cttgtgcggc ctcttttgaa
    12661 tctggcaata ttgatatttt tcaatacttg ctttcaaaag gtgtttcaaa agacactatg
    12721 cctatgacac gcgattatat gtattctgct aattgatata gttaatatta tagtattcca
    12781 acttattcta aataaaaagg actctcttga attcaaggga gttctttaac gttcttctct
    12841 ggctaactct atacaatgtg acttttggtg acaatgtgga cagaatactt ttcgcgtctt
    12901 aggggtatgt ttcgctagat taaatgcttg atatgtcggt gtaaaggttt gatgacaatt
    12961 tggacaaaga taaacaacct gttgtttaaa ataccaaacc ataccattca tagctattaa
    13021 gaatactgtt actgctatcc acataagcca tctttgattt agagaaactg ctgctattat
    13081 taacgtaata tagaccataa taatcataac gacttgtagc actatgcttt tttgtagtat
    13141 accccatttt cttttgtttt cttccataag cacaatgtca gaaagatagc ctaatgtttt
    13201 actatcttgt ttttctacag tttttagtag gttgacagct ttgtcaagtt tgtctttttt
    13261 actatcaata gctagcctta attctcttat ctacacttgt aaaaaaagct ctaaaatctg
    13321 gctagcattg tcttcagtaa atagattttt gatattatca attgaaaaat ctaaatctcg
    13381 taaataaata atttgtctaa gcttttctaa atcagcaata ctataaaccc tcctacctcc
    13441 ttcagatagt tctgatgggg ataaaattcc tcttttgtca tagtactgaa cagtacgaac
    13501 agtaactcct gcttctttag ctaagtcacc agtggaatat ttttctgaca tgtttcacac
    13561 ctcctttaat atagtaaaca cttctttatc agaaacacct ttactatatc aaatgaccta
    13621 aggtaataag caagtaataa cgctaagtat ttttaacttt aaataaaact acaacaatta
    13681 atcaaacaaa tttcctaaag tcgcaaatgg gctatcaact gctacctctt tttgctgttt
    13741 taaatagatt ttattttcat ccaagtccag aaatttttca taaacacgcg cagtcatacg
    13801 ggcatcctct aagctattat gtgctttacc ctttattcct aaaaagtcag caacagtagt
    13861 caatttaagg ttgacaatac catttaaatc tgtactgcgt ctcacaaaag cttcatcata
    13921 taagtccacc tgatattgac tagttagatc caaaccattt tcaactaaaa ggggtaaatc
    13981 cgatttatac ccattataac caatcagtgg tgtatctcca acaaaactct gaaagtctgt
    14041 caaaactatc tcaattttag gagcccctat aatatctcta gctgtaatac ctgttaaacc
    14101 attaataaag ctttgtaatg gaacttttgt gtgaacataa gtatcaaata aagcaatctc
    14161 ttgatgatta gaatatttaa cggccgacac ttgaataata tggctatgct caccaaccgt
    14221 gttaaactct aaatcaaaag ctatatattc ttgtaattgt ttcattattt ctcctaaaaa
    14281 gcgcaaggaa actccttacg cttttattct attttttatt gaagaaacgt ccaaagaaac
    14341 tttttttaac agcttctaat tcttgaattt tttcttcttt ggcttcttga atctcctcaa
    14401 cctctgcttt agcttcattt aactcaagtt gcagacgttg cgtatcttcc atagcagtta
    14461 aagtcaattg ctgttgttga tctaattgct tatctttctc tgctatttga atatctttta
    14521 catgtaactg ctcgtctttt gaagcaatct gaatatcttt tgcttttaac tgttcatata
    14581 aacgtgtgat ttcagtattc ttctcatcaa cgagaatttc taaaagttct cgtcttgatg
    14641 cttcttcgtc aattggttcc tcttccaaaa tagttttttt atagatttct tccaatttaa
    14701 tcaagccatc acgattaaca actgtaacgc ctttactatt cttttccaca cattcttcag
    14761 ggagtgtttt aactctgtta ttcatcgctt gacgactaac ccctaaaatt tcagctaatt
    14821 cactaaccgt cttttcaact gtcataactt cctcttttgt actgattttc ttataaaaga
    14881 atatcacaat cctaatctag tgtcaattta ctgtcaactt aaagtgtcaa acaagacatt
    14941 ttgacgtcta tttctctatt tatatgctag aataagatta tgaagcatta tgacacaata
    15001 attataggtg gtggacctgc tggaatgatg gcagctattt cctcaaattt ttacggcaac
    15061 aaaacacttc ttattgagaa aaataaacgt ttaggcaaaa aactagctgg aacaggtggt
    15121 ggccgctgta atgttaccaa taatggaaac cttgatgagc tcttagctgg tatacctgga
    15181 aatggccgtt tcctatatag cgtcttctca caatttgata accatgatat tattaacttc
    15241 tttcaagaca acggcgtcac acttaaagaa gaagatcatg ggcgtatgtt ccctactact
    15301 gataaatctc gtactatcat taacgccctt gaaaataaaa taaaagaact tggtggtcaa
    15361 attatgactg acacagaagt tgtctctgtc aaaaagatag gagattcatt ctatatcaaa
    15421 actaaagata cacagttcgc atccgataaa cttatcgtaa caactggtgg taaatcctat
    15481 ccgtctacag gttctactgg tttcggtcat gacattgctc gtcactttaa attagaagtc
    15541 acagatatgg aagccgccga aagccctctt ttaactgatt ttccccataa aaaactacaa
    15601 ggtatctcct tagatgatgt cactttgtca tttgaaaagc atattattac acacgatctt
    15661 ctctttactc attttggatt atcaggacca gcagccttgc gaatttctag ctttgtcaaa
    15721 ggcggagaaa ctatatacct agatgttttg cccaatatct ctgttaaaga attggaaata
    15781 cacttccaaa atgaaagaga aaaatctttg aaaaacgctc ttaaaatact tcttcctgag
    15841 cgtttagctg aattttacgc tgaagactta cccgaaaaag taaaacaagt atctgtaaaa
    15901 gacttagaaa tgttaattca aaaacttaaa aaacttccta ttttagtaac tggcaagatg
    15961 tctctagcaa aatcctttgt aacaaaaggg ggagttgatc ttaaagaaat aaaccccaaa
    16021 acacttgaaa gtaaaaaggt agctggttta cattttgctg gtgaagtcct agacatcaat
    16081 gctcatacgg gagggtttaa cattacttct gccttatgta caggctgggt agctggtagc
    16141 ttacactatt aatacatgaa agctgaacaa ttccgtcagc ttttttatat agaatttaaa
    16201 gataatattt ggctcttaag gcttctttga ttttttccaa ttcatctggt aaatttacct
    16261 gatgaatagc atacccaatc tgataagggg atactataaa acgaggaaca attttacaaa
    16321 taagaccccc atcatgctta tagaaaaaga gattataaga tttacagcgc cctcccaaga
    16381 attcatttaa aatataatga cacaaaattg gtaaactatc tgcaatcaaa atagcgtttt
    16441 ccaatttgga tactttaatt gtgaactctg tgaaatcaag cataggacga ttggcaatac
    16501 taatctctgc ttgttgactc gtttccgaac aaacctcgat accatcaaac caatctaagt
    16561 tcaacgtttc ataagcatct acgtttttta agccaacaat ttggctatga ggatgtcgta
    16621 aacttccgcc agataagggc ccaaaatttc gataaagaac gaccgaacgg taatgaccac
    16681 tttcttccat taattgccat tttgacaaaa gataatctaa caattggcgc atctttgact
    16741 cactataaac tgaaatatcc ccattgtgat catttgattc aatcaccaat gtttgatagg
    16801 tttcttctaa agttgggtat ttattttcta cccagatcat atcgtctttc acgtctagga
    16861 tttttcctaa ttggctttta tcacaaaatg gacatttgtc tttattgctg ggtttttgtt
    16921 tcgcgatatt agggttaaat cggatatatc tttcattcat actgttattg taacacattc
    16981 atcaccatta caatataata cttatccagt taacattttc cttgcattct tagctgaaaa
    17041 gagtataata gaatcatgtc ttgtttaccg gttaattacc agttaactag gcaccctgtt
    17101 ttgggataga aaaatctttt tgggaaagga gaaatcatgg ctaagaaatc aaaaattgct
    17161 aaatttcaaa aacagcaaaa actcgtagaa caatatgctg aactacgtcg tgagttaaaa
    17221 gaaaagggtg attacgaagc acttcgtaaa cttcctaaag attctaaccc taaccgactt
    17281 aaaaatcgtg atttaatcga tggccgtcct cacgcttaca tgcgtaaatt cggtatgagt
    17341 cgtattaact tccgtaacct agcttacaaa ggccaaattc ctggcattaa aaaagcaagc
    17401 tggtaattcg tgaagacaaa tagttacttt ttatgactat tctattgagt gtggacgaat
    17461 agttaataca ttgtaattat ttaacctgtt gagtatttac tcagctatta caaacttctt
    17521 caaaatttta ttatcatagg aatgtaggat ctcatcaatc cttaaatgac taataataaa
    17581 agatgaaaaa atttttctta tctcaaatca tagatgccaa tttatttatt ttcaaaaagc
    17641 ccggtatgcc
//
LOCUS       DATRME010000038        17600 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRME010000038 DATRME010000000
VERSION     DATRME010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17600
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1330)
                     /locus_tag="VBC23_001245"
     CDS             complement(<1..1330)
                     /locus_tag="VBC23_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843193.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKR"
     gene            1622..2293
                     /locus_tag="VBC23_001246"
     CDS             1622..2293
                     /locus_tag="VBC23_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843194.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(2364..4364)
                     /locus_tag="VBC23_001247"
     CDS             complement(2364..4364)
                     /locus_tag="VBC23_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6843195.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(4499..5259)
                     /locus_tag="VBC23_001248"
                     /pseudo
     CDS             complement(4499..5259)
                     /locus_tag="VBC23_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(5321..6313)
                     /gene="pta"
                     /locus_tag="VBC23_001249"
     CDS             complement(5321..6313)
                     /gene="pta"
                     /locus_tag="VBC23_001249"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6843196.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(6339..7223)
                     /locus_tag="VBC23_001250"
     CDS             complement(6339..7223)
                     /locus_tag="VBC23_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6843197.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(7220..8056)
                     /locus_tag="VBC23_001251"
     CDS             complement(7220..8056)
                     /locus_tag="VBC23_001251"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6843198.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(8031..8702)
                     /locus_tag="VBC23_001252"
     CDS             complement(8031..8702)
                     /locus_tag="VBC23_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6843199.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            8812..9384
                     /locus_tag="VBC23_001253"
     CDS             8812..9384
                     /locus_tag="VBC23_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6843200.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            9561..10535
                     /locus_tag="VBC23_001254"
     CDS             9561..10535
                     /locus_tag="VBC23_001254"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6843201.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            10539..11654
                     /locus_tag="VBC23_001255"
     CDS             10539..11654
                     /locus_tag="VBC23_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6843202.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            11656..12003
                     /locus_tag="VBC23_001256"
     CDS             11656..12003
                     /locus_tag="VBC23_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6843203.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            12158..12796
                     /locus_tag="VBC23_001257"
     CDS             12158..12796
                     /locus_tag="VBC23_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6843204.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(12809..13489)
                     /gene="radC"
                     /locus_tag="VBC23_001258"
     CDS             complement(12809..13489)
                     /gene="radC"
                     /locus_tag="VBC23_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6843205.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(13585..14718)
                     /locus_tag="VBC23_001259"
     CDS             complement(13585..14718)
                     /locus_tag="VBC23_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6843206.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            complement(14809..16248)
                     /locus_tag="VBC23_001260"
     CDS             complement(14809..16248)
                     /locus_tag="VBC23_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6843207.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGLIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            complement(16408..17022)
                     /locus_tag="VBC23_001261"
     CDS             complement(16408..17022)
                     /locus_tag="VBC23_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6843208.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            complement(17123..>17600)
                     /locus_tag="VBC23_001262"
     CDS             complement(17123..>17600)
                     /locus_tag="VBC23_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6843209.1"
                     /translation="ERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENL
                     VSEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFG
                     DGGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
BASE COUNT         5895 a         3428 c         2714 g         5563 t
ORIGIN      
        1 ttctttttcg aagataagtt aatcctacaa tagctaagat aacaacaacg ataacgaata
       61 gaactgcaaa ttgactacca attatagctc catttttcgg aatagctact tgtccgatgg
      121 taaaaatttg tgaggctact gcggttgcaa aagctcccgc aaattgttgt agtgtattta
      181 agatactatt accatccgct gactgtgctg gctttagttt tgttaaagct gtcgtaacca
      241 tattacttgc tccaatcccc ataccaattt tagtaagaat atcaaaggct aaaagtacta
      301 caacaaactt cgcaggaatg aatatagctg taccaattaa acctatcaaa gaaagggaga
      361 tacctatcat aattggctta aatgcaccaa ttttatccag gagataacct gcaaaagggg
      421 ataaaaaaac tactgctatt gcacctggaa acataaacaa accagctttg gttgaagttt
      481 gatgtaaaac aagttgcaag aggttaggta ataagaacga aagtgctaga ggaatcattt
      541 gaaaagccag gacgccatac aataagattg agaaatcttt atctttaaaa acattcaaat
      601 tcaaaaattc tagctgttta tttaaatagt aaaaaataac aaaactaaca atagctacta
      661 ataagtaaaa aatagacatc ttttcaatag ctaaaagaag ggttgccaag ccaataccta
      721 aactgacaaa ggcaggaaaa ttgagtttat cattaattct tacttgacgt ttgggaatag
      781 aagccaaacc tataaaggta gatatgataa gtattggtgc cagaagcata aaaatcatct
      841 tccatcctaa cattcctgaa atgacaccac cataagtagg tccaactgcc ggtgctaaaa
      901 gtgtgaccat agcagctact cccatcatta gtcctctacg ctccatcgga atttgtgtaa
      961 gaatgatatg aaacaataaa gggagggcta atcctgtccc aacaccttgc aagactctcc
     1021 ctactaacaa tatagctaaa ttaggggcaa agctatcaat taaaacacca aataaaaaaa
     1081 gcacatttgc caatgtaaaa atttgtctct ctgataaatt tttaatcata aatgctgaca
     1141 aaggcacagt cacagcaaca gctaacaaat ttcctgttgt cacccattga ataacagcgg
     1201 ggttaacccc aaattctttc atcaaaagcg gaaaagtgac attcatagat gtttcaataa
     1261 gaatccctga aaaacctaat atagccgttg taattatggc aggcataata cgccatccct
     1321 ttttagtcat taaaatctcc ttactttgat tattatcata aaatagagca ccccgctaaa
     1381 tcaaggtgct ccatttaatt cattcgaatt aaaattaaat tgtttatatt agttgtcaaa
     1441 ggacaatcaa cagctcttat tatatcaaat aaagctaaga aaaacaaaga gttttttgaa
     1501 tattaatgcc ttcactttct tatctattct ctacccatca atcaccactt cagagaacat
     1561 aacaattgca atttactctt aataccgtta tcatgttcat atgctaatac ccaagaaaat
     1621 agtggctttt gcctcaaatg aatctgaaaa taatttcttt attacgttaa agacttactt
     1681 taattatttg ttttctattc aaatcattac agatatttca actctcaatc atgcagattt
     1741 tgacggtagc tttgcattcc atgatataga aactagcata cctcatctgg taattgactc
     1801 caactactta gcgattagtc agactaactc aaaaacagag gctaacgata ttaaaacgtt
     1861 ctcggaactc tccaaaacaa tgaccgagtt ttactatatg ctaaattttg acttatttaa
     1921 ccatctccca tatcgtttta gattgcataa taaggacggt caaacaattt attcgaacca
     1981 taaacctgaa gatccttttg atatttatcc tgaagaagaa tatcctatag acaaatgggt
     2041 tcaaaacagt ctaatagaga aaaaagcgaa agagctacat ttactcttac ctggcgcatc
     2101 acaagattat attctagtac aatcttataa acgcttagaa aatgatagtg ggcaactagt
     2161 tggttatatt gaacatgtcc ataatattaa accactatta gaggggtatt taaaagaatc
     2221 cggtcaagct atcgttggat ggtctgatgt cacgtctggt gcttctatct ctaacgatga
     2281 ttttgaacta taatccactc gtcactctaa aaagaaaact aataactaaa aggcactggt
     2341 ttaattccag cgccttttat taatcaatct ccagttgttg taagttcaat tttttcttta
     2401 attggtagac ctcgattagc tgtaaaaatc aaaggaacta cttctgttgt aacttcggaa
     2461 aattgtagac caaaccatcg tattggtgtt gctgtccaat gctttacaga tgatttcatt
     2521 agcataacaa atcgttcacc aggttttagg ttttgactgc ttgaaaaacg ttcatcaagg
     2581 acaataaacc taaaatctcc tactttacta tcaggtctaa cgctgtatgt ctttccttgt
     2641 ttaggcagac gtccactttc aagtaattct tctacaatcg tacgaagata gcgtgacaca
     2701 gtctgacgca tcttaaatcc aaggtaaagt tctaccttca caatgaagtc ggttcccatc
     2761 atgtcaactt tatactcagc tgtataaggt tcatcagtga ccttaatgtt tacaaaccaa
     2821 taaacttgtg cttttttagg gcgcttatca aggatagaat acatgataga cttatcaatc
     2881 atgtctcctt ccatacgatt tgtcaaataa acaacgttag tatgataaat cggataacga
     2941 tgatcatgtc taagtttatc taattgtccg atgtaatctt ttaaatccaa caacttaaca
     3001 taacgcgaaa caattttact acctttatac cagatagtca tgatgaatat aattgctact
     3061 gctataataa caacaacata gcctccatgc ataaatttaa cagctgaagc aaggaagaag
     3121 attccctcta aaataccaaa gaaaatcatc atcaataaga ctaaacctct ttttactcct
     3181 ttttgaatta aaaagaaaga cagtaaaata gttgtcatta gcatcgtaat tgttatcgct
     3241 aatccatatg ctgcttccat gtgcgctgaa gttttaaaaa gcaaaacaat agaggttgta
     3301 atggcaaata agaaccaatt aataacaggt atgtaggttt gaccaatatt gtcaccagga
     3361 taagttgaac gaaattgtgg gaatattttt agtcgcatag cttcagaaac taaggtaaat
     3421 gatccagaaa tcagtgcctg tgaagcgatg atagctgcca aagtagctaa aataacgaca
     3481 tgcattgtaa attgcgaagg aatactagca aaaaagggat tcaattcatt tcctgcgttc
     3541 ttattagcta aaatccatgc cccttgccca caataagaaa gtataatggc aaccttaaca
     3601 aacggccatg aaacatgtat atttccacgc ccaacatgac ctaagtcaga gtatagtgct
     3661 tctgctcccg ttgtcgccag gaaaatagat cctaaaataa aaataccttt atgattctct
     3721 ggactaaata acaatttcaa accgtagtat ggattaattg ctttgaaaac ttctggatgg
     3781 gcaaaactat taaggagacc gctaatacct aaaaaggcaa accatataaa cataattggc
     3841 ccaaataatt tcccaataac acccgttcca aacctttgga tggcaaaaag taacagtaaa
     3901 ataaacaagg tcgcaaaaat aacattactc tgattctgga aaatatgctg caaactagga
     3961 actactttta atccttcaac ggctgaagtt acggttacag ctggcgtcaa agctccatct
     4021 gacaacaagg ttgcacctcc aataacagcc ggaacaatta accaaggtgt catttttcta
     4081 acaagggtat ataaagaaaa aataccacct tcgtggtgat tatccgcctt taaagctaca
     4141 agcacatact tgatagttgt aataagtgtc aaggtccata tgattaaaga tatagaacct
     4201 aagataaacg attctgtgac actagaaata ccaccttggt tttcaaccaa tgattgcatc
     4261 gtatagagtg ggcttgtacc aatatctcca taaacaatgc ctaaagcaat aataaatcct
     4321 gcttttgatg ctttatcaaa agaagaatga ttgacatgtt gcatttctgc caaccctccg
     4381 attaaataat attagtcaaa aaaatggctg aactcattat agcagattaa gttaaaaaaa
     4441 caacacttat ctataaaaag accagaaatt attaaagcgt ttcctggtct ttactatttt
     4501 aatcacgata aacaggttga ggtccataag tttgactgac tggcattatt tctatcgatt
     4561 aatattaatg tgttctggtt ggctagcaac ccatgacacg gtattagcaa tatcctctgc
     4621 ttgaattgca tgcgcacctt cataaagttt ttcaactctt ttatgatctc ctttgaaacg
     4681 tacagttgaa aattctgtcc cttcacataa accgggttct aggttagtaa ctctgatttt
     4741 agttccagct aaatcggcac gcaagttgag agcaaattgt tttacaaaag cttttgatgc
     4801 gccatagaca ttagctcctg gataaggaat tgttcctgca gttgatccca aattgataat
     4861 taaccctcta ttaacttcta ccattttcgg taaaatgcat cgtgtcaagt aaattaatcc
     4921 tacaacgtta gtattaatca tagtcatcca atcttcaaaa tcagcttcat atgatttatc
     4981 caatccaagt gctaaacctg cattattaac caacaaatca attgattgca aatttttagg
     5041 cagactttct aaagctttgt caaccgattc tctacttgta atatccattt aaagtggata
     5101 aaagtaaccc tctgaaaact tttctccaag gcattttaat ttatccattc gacgagcaca
     5161 accaataacg ctataaccat ctgaaactaa tttagtaaca acagctgcac caaaaccagc
     5221 agatgccccc gtcacaagcg caactttacg tgatatcata aatactcctc ctctagtata
     5281 tagaaaaacg agctaggaaa aatccttctc gttttataac ttattttgat ttctcatcaa
     5341 tagcttgagc agctgtaatg atagcaagtt tgtaaatatc ttcagcactt gatccacgtg
     5401 ataaatcatt tactggagca ttcaatcctt gtaagatcgg tccgattgct tcaaacatac
     5461 ccaaacgttg tgcaatttta tagccaatat ttcctgattg aaggtctggg aaaataaaaa
     5521 catttgcttt tccagcaaca tcactattag gagctttaat ttctgcagtt tccggaacaa
     5581 aagctgcatc aaattgtaat tctccatcaa cagctaattc gggagataaa tctttggcaa
     5641 ttttagctgc ttcttgaact ttttcagctt gaggcgcttt tgcacttcct tttgttgaaa
     5701 aactaagcat agcaatcttg ggattaatat caaatatttt ggctgtatcc gcagtattta
     5761 ctgcaatttc tgctagctct tgtgcattag gatcaatgtt aatagcacag tcagcaaaaa
     5821 tataacgttc ttgtgtattt tcacggttca tcaagaatac acctgaagtg cgtgaaattc
     5881 ctggttttgt tttaataatt tgaagagcag gacgtacagt atcagctgtt gaatgaatgg
     5941 cccctgatac cataccatct gccaaaccaa gttttactaa cataacccca aaatagttaa
     6001 catccttcaa aagacgatct gcatcttcca tagtagcttt gcctttacga atctcaacaa
     6061 aagcttcttt catctcttca aatttttcgt attgttcagg gtcaataacg ctataatctt
     6121 gatcaacaaa accaagtctt gccaaagaat cgtgaacttc gtcagcctta cctaaaataa
     6181 caggatccac aagaccttca aatttcaaac gagctgctgc acgtaataca cgttcatcgc
     6241 ttccctcagg gaaaacaatc ttaacataat cacttgcaac tttttctctt aaaccaccaa
     6301 ataaggaacg catatatata aaaccttcta tttctaaatt aatgtaattc catgataacg
     6361 ctgtcaaagt catctgtcaa gtctagcgtc tgtttattta tttttcccat aaaagggtcg
     6421 taaaaagaca aagaatgaca atgcaatgct tgcctgttta ttcctagatc catacgtcct
     6481 ccgtacaaat catctcccaa aagaggaaag ccgatatgag caaagtgaac tctaatttga
     6541 tgagtacgcc ctgtatgtaa tttgatatcc actaatctaa catcaccata acgagcaact
     6601 acttgataac tagtatgtgc atatttgcct gattcatgga ctcgcctcgt aataatacta
     6661 tcaacatctc gtgcaatagg agctataata tcaccactat ctgctaaatc accactccct
     6721 gatactaatg cataatacct tttttcaatt gctttagcct gaagttgttt atctagacga
     6781 gcatgggcat aaccatgctt ggcgaataac attaatccac ttgtatctcg atccaaccgg
     6841 gtcacaatat ggacctgctg attggcatag ttattagaca cataatagtg ttttataaaa
     6901 ttagcaattg tatttgaatg aatagagcta ggaatactag cgaaaccaaa aggtttattt
     6961 aaaatcaaaa aatgatcatc ctcataaaca atatctaaat cgtgaggtac tggttccaaa
     7021 gtttcgtgag aaggctcgtc aggaatatct atcgtaacaa catcgccaat ctctaataag
     7081 aaaatagcgt tctgctcttc accattgaca aaaatcttac cacctctgta cttaatcttt
     7141 gctaataacc cccttgaaac atcatgactt ttcaaaagtg ttttaacttt gcacctttct
     7201 tctgctatgt attcaaattt caatgttcag gttctccgat aaaagcatcg ttaacacgtt
     7261 cccaaaagct agtgtgactt ggagttgaaa caaagttaat acttttttcg tcaatactat
     7321 attctatttt ggtaacattt ttgtaatgga ccgttttgtt atctatagaa attgtataga
     7381 caccaactcg tttaggaact atctctatag catctttttt ggggatgata accgatgacc
     7441 ccaaggtacg atagacgcga ttattaagac tagaaatttc tgttagttgc aaggcttcta
     7501 tcgttgggtg gagtactgcc ccccctagtg atttattgta agcagtacta ccggttggtg
     7561 tagaaactaa aatgccatct cccctaaacc tttcaaatac cacttgatta ataacaacat
     7621 ccgctaccat tgttttttca atacgcttaa tagtagactc atttaaagca cgcgcacgaa
     7681 taactcgacc atcttcaagg gtaatagtta ctttcaaaat aggataggag atttgttcac
     7741 ctttatcatt tttcaaatta ttaattaatg tgtcaacttc aaaatctcga tagtctgtat
     7801 aaaaccctaa atgtcctgta tgaacaccga caaaccttac tttatccagt tgtttttcgt
     7861 acatatggaa ggctgacaaa agcataccat ctccaccaat agaaatgaca atatcaggat
     7921 ccttttttga taaataaaaa tcaggatcat gtttaaaagc agcaaagagc tttgaggcta
     7981 cacgcttgct ttgatatttt ccgttagcaa ttatagcaac acgtgttgct ctatctgtaa
     8041 aattcatctg tgtcatcact atttcctaca ccatcgctca attttcgatt taaaggatca
     8101 aataaaagct gtgcttcacg aatatcttcg cgaatcttac gcatttcttc atccaattct
     8161 aaagcgattt ttgctgtttt ctccagtctt tgtttaattt cttcagggaa gtccccctga
     8221 tacttgtaat ttaatgaatg ttcgatggtt gcccaaaaat tcatagccag agttctaatt
     8281 tgtatttctg ctaaaacttt cttttgtcca tctattgtgt ctacaggata ctctacaacg
     8341 acatgatatg aacgatatcc actagatttc atatgggtaa tatagtctcg ctcctgaacc
     8401 actgtcatgt catgacgttt tctcaacaag gcaaggactt catcaacgtc atctacaaat
     8461 tgtaccatga tacgaagacc tgcaatatct tgcaagtcct gagcaagatt ttcttctgaa
     8521 ataccacgta aaaccatctt ttcttgaata ctttcaaccg actttacgcg tccagtaaca
     8581 aattctattg gagagtgacg attttgcttt ctaaattgtt tacgaatacc acgaagcttt
     8641 atttttaatt caccaaccgt ttgaatataa gggtctagaa atgtttccca atccattgac
     8701 attctaatcc tccaaaatat ttaatgtact ctcttgtaaa ataaactttt tttctataaa
     8761 attaacacaa caattaatat tataccatat ttaggaaaag gaagtgataa gatgacacac
     8821 ctagaaattg aatacaaaac tctattgaat aaagacgaat ttaatcgtct aacaagctta
     8881 ttttctcatg ttcaacctat tactcaaacc aattactatt ttgacacaga aacatttgaa
     8941 atgaaagcac accgaatgtc acttcgcatt agaacgttac ctaaccgtgc cgaactaaca
     9001 ctaaaaatcc ctagagaagt aggaaattta gagcacaatc atgaccttac tttagaagaa
     9061 gctaaatata tcgttaaaaa tggccagttc cctgaagata ctgagattgc ttctttaatc
     9121 cttgaaaagg gagttgatcc aaccaaatta gctgtctttg gacaacttac aacaacaaga
     9181 cgagagatgg aaacttcaat cggcttaatg gctctagaca gcaatatata tgctgatatt
     9241 aaagattatg aacttgagtt agaagtcaaa caacctaaac aagggaaaag agatttcgac
     9301 cagtttttaa aagaaaataa tattaatttt aaatacgcca aaagtaaggt ggctagattc
     9361 tctgctactt taaaaaataa ctaacgttga gagattggat tttccaatct cttttataat
     9421 tctcatttaa gcaagaactt aaaatctcat actatctctc ctcaacattg ttattgcaag
     9481 cattttcatg tttcttatcg tgaatttttt ctattttttt ggtaaactat tagtatcaat
     9541 ctaaaggagt catctatctc atggcagaac aatacgccga taaacaaatc aaacttttct
     9601 cattaaccgc taatagagaa attgctgaaa aaatttccca agcttcagga attcctttgg
     9661 gtaagatgtc atcacgtcaa ttctctgatg gcgaaattat gattaatatt gaggaaacgg
     9721 tgcgcggtga cgatatctat atcatccaat ctacaagctt cccagtcaat gataatctct
     9781 gggaattact cattatgatt gacgcttgca aacgtgcaag tgctaatact gttaacatcg
     9841 tagtccctta ttttggatac tctcgtcagg accgcatcgc tgcatctcgt gaacctatta
     9901 ctgcaaaatt ggtcgctaat atgttagtta aagctggagt tgatcgagtt cttaccctcg
     9961 acttacatgc agtgcaagta caagggttct tcgatatccc agttgacaat ctttttactg
    10021 tcccactatt tgctgaacac tataatcaac tcggtctctc tggtgaagat gtcgtcgttg
    10081 ttagtcctaa aaattctgga atcaagcgtg ctcgtagttt ggcagaatac cttgactctc
    10141 caattgctat cattgattat gcacaagatg attctgaacg tgaagaaggc tatattattg
    10201 gtgaggtaga aggtaagaaa gctattatta ttgatgatat tttaaataca ggaaaaacct
    10261 ttgccgaagc tgcaaaaatt ttagaacgtg gtggagcaac tgagatttat gctgttgcta
    10321 gtcatgggct ttttgctggc ggcgcagctg atattttaga gtctgcacca attagagaaa
    10381 ttatcgtaac agactctgtt ttatctaaag agcgtattcc tagcaatatt aaatatctga
    10441 ccgctagcca tctcattgca gacgctatta tccgtatcca tgaaagaaaa cctttaagtc
    10501 cattattttc atatcgttca gataagaagg attaattgat gatttattta gacaatgctg
    10561 ctactaccgc tctaacccca tctgttattg agaaaatgac caatgtcatg acaagtaact
    10621 atggtaatcc atctagtata catacctttg ggcgtcaagc aaatcaactt ttacgtgaat
    10681 gtcgacaaat tattgctgaa tatctaaatg ttaattcacg tgaaattatt ttcacttctg
    10741 ggggaactga gagcaacaat acagctatca aagggtatgc tcttgcaaat cagctaaaag
    10801 gtaaacatat tattacctct gaaattgaac atcattcagt cctacatact atgacttact
    10861 tatcagagcg atttggtttt gatattactt acttaaaacc aaaccatgga caaattactg
    10921 caaaagacgt tcaagaagct ttacgagatg atactattat ggtatctctc atgtttgcta
    10981 ataatgaaac cggggacttt ttaccaattc aagagattgg tcagcttctc aggaaccacc
    11041 aagctgtttt tcacgttgat gccgttcaag tctttagcaa aatggaactt gatcctcatt
    11101 ctttaggaat tgacttttta gctgcttctg cccataaatt tcacggtcca aaaggtgttg
    11161 ggatacttta ctgtgctccc catcactttg atagtctact tcatggtgga gaccaagagg
    11221 aaaaaaggcg tgcttcaact gaaaatataa ttggtattgc tggaatgtct caagctctta
    11281 ctgatgctac gactaacacc cttaaaaatt ggactcacat tagtcagctg agaacgacct
    11341 ttttagatgc tatttcagac cttgacttct atcttaataa cggtcaagac tgcttacctc
    11401 atgtacttaa tataggtttt cctagacaga ataatggctt gttattgaca cagttagatt
    11461 tagctggatt cgcagtttca acaggttctg catgtactgc aggaacagtc gaacctagtc
    11521 atgtcttaac aagcttgtat ggagccaact caccacgtct aaatgaatca atacgtatta
    11581 gtttttcaga actaaatacc caagaagaaa ttcttgaatt agctaaaacc ttaagaaaaa
    11641 ttataggaga ttaatatggc atttgaaaaa gaaataaatc ttccagactg caattacatt
    11701 tacactatca gcccgaacat taaaaaatat accttacgcg atactacatt cactcaaaat
    11761 cgcttaggta attatgaatt agagcgtctc ctagaaacag tacctaattc tggcgatggc
    11821 tttcctctaa agattacaat caataaagaa ctttctggtt ttaagctctt aattactgat
    11881 aaatctggcc ttagaatagt aaatatcttt aaatctaccg atcataaaat tcttcaagac
    11941 aaattttatt tcttaatgga cagcctcgtt gagcgtaata tctttacgaa aactgcaaaa
    12001 taatttaccg accactattt ttaacatttt agtggtcttt ttgcgaacca ttttgttgtc
    12061 actttcacaa tctttttgta caatagagat gtgaagaatt aagttatata actgattcta
    12121 aatcgaataa taaaattttt aacaaggaga taattttgtg attatggata agtctattcc
    12181 taaagcaact gctaaacgtt tatcactgta ctaccgtatt tttaaacgtt ttaatactga
    12241 tggcatcgaa aaagctagtt ccaaacaaat tgcagatgcc ctaggtatcg attctgctac
    12301 tgttcgacgt gatttttctt attttggtga actaggacgc cgtggttttg gttatgatgt
    12361 caaaaaactt atgaacttct ttgcagaaat attgaacgat cattctacaa caaatgttat
    12421 gctggtgggg tgtggaaata tcggtagagc tctcttgcat tatcgtttcc acgatcgcaa
    12481 taaaatgcaa atttcaatgg cttttgattt agatagcaat gatttagttg gtaaaacaac
    12541 cgaggatgga attcctgtct acggtatttc gactatcaat gaccatttaa tagatagtga
    12601 tattgaaact gctatcctaa cagtacctag tacagaagcc caagaagttg ctgacatctt
    12661 agtcaaagca ggtataaaag gcatcttgag tttttctcca gttcatttaa cattaccaaa
    12721 agatatcatt gttcagtatg tagatttaac aagcgaatta caaactttac tttatttcat
    12781 gaaccagcag cgataaatag ttcattattt ataaaatatc tgcttcttct cgaaaactat
    12841 aatatttatt ttttccaacg atgatgtgat ctaggcagac aattcccaga tgatcacatg
    12901 atcgttttat ttttttagtg aaacttaaat cactttcact gggatttgga gaacctgagg
    12961 gatgattatg tataataatc aaagaagttg ccatgttttt acaagcataa tgtagaattt
    13021 ctcttggctc tgctactgaa cgacgtacag taccaataaa aatagttctc tgttcgataa
    13081 tacgattttg tgtatccata taaatagcta ctaaatgttc ttgtttttta tcccctaatt
    13141 cgagcatcat tttcctcgct aattgttcac tacttaaaat ttgctcttta cgatcatatt
    13201 cagctttgtg aatccgtttt gctagttcta gcatagcttt tatttcgacg gatttaacct
    13261 gaccgattcc tttaatggat tgcaactcct gtaaggataa ttgaccaaaa tctgctaaac
    13321 tgcttatgtt ttctaaaatt tgcgttgaaa tttcaagaac aggtttttct ttaatacctg
    13381 tacgtaagag aatggctaat aactcctgat tactcaatct atctgcgcct aaatcaacta
    13441 ggcgttctct tggtagtaaa gcttcctttt ttaattcaat atggtacata aaacacctcc
    13501 agtcataata ttcgtaaact aatcaaaaac tcatgtttta aatcaataaa aaatacttag
    13561 atgaaaaatc taagtatttt ttatttaacg ataggttcgt gcacgatttc taagcgtttg
    13621 tcgcttgata atatgatctt ttacaatctg atagatagta gctgcaacag gaacagcaag
    13681 taacatgcct aagatccccc ataaagcacc tccgatagta atagccatta aaacccacat
    13741 agaaggcagt ccaatcgatc caccgacaac tttcggataa atgacatttc cctcaaattg
    13801 ttgtaaaagg atcaagaaaa gaacaaacaa gaatgcttca gtaagcgatt cagtagcaat
    13861 taagatgaaa ccgattgtaa caccaatgta ggctcccaca accggtatta gagctgtaaa
    13921 agcaactaaa accccaactg ttaaagcata aggaaattgg aagatcaaca taccaataac
    13981 cgtcaaactt cctaaaatca tagcttctaa agtttggctt acaaaaaaac catggaaacg
    14041 ttgatgaagg atatgacgaa cgtaatggaa tgttttgcct gttgaaccta aataggtatc
    14101 aattaacaaa ttaaattgac gtcccaactg ctccttgttt gccaaaacgt aaattgaaaa
    14161 aataaaacta acaaaaacat tcagaagtgt tgccgcaata gaggaaactg aggttagtaa
    14221 atttgttaaa acagataaaa cttgcttcaa aatctgctgg ctataaccac ttaaagtaga
    14281 aactaagtct ttatctgttc ccatataatt taaagcctca gaaatttgtt tattttcatt
    14341 gagattatta accaatttag caagtgctcc ggtatcaata accaataaag aactcaaact
    14401 agaaatcaaa tctggaatga caattgaaaa tagccagaca attaaaccaa taaaagttgc
    14461 ataggataaa atcatagaaa cactacgctt gattgccatt aatagtctag atcctttaaa
    14521 aagctttata tataatcgct catagactga cattacaata ttaataatat aggcaattcc
    14581 agctcccatc aaaaaaggaa atattgcctt atgaagcgta gttaaaatat tagaaacagc
    14641 tccccaataa gcctgtatag catagcaaat agcaaatgtt atgacaacat aatagacctg
    14701 ccgtttttca aatttcatga aatctcctta ttaatcttaa tttctttcaa ttattataca
    14761 ctattttatg aatgagaaaa agagagttaa cagttattag tctgctcttt attctatttg
    14821 actgccatta cttgcaataa ccttcttgta ccaataaaac gattttttct tgtaacgctt
    14881 gagtgttccc tctcctttat tattgcgatc aacataaatt aaaccatagc gtttttccat
    14941 ttcaccagtt cctgcagata ctaaatcaat acatccccat gtagtatagc caattagatt
    15001 aacaccatct acatatatag catctctcat tgcagcaatg tgctgacgga ggtaatcaat
    15061 acgataatca tcttctacgt aaccattctc atcaggaata tctactgctc caagtccatt
    15121 ttcaacaata aacatcggtt tttgataacg atcccagata gcatttaagg taatgcgtaa
    15181 tccaagcgga tcaatttgcc atccccattc agaagatttc aagtaaggat tcttaagaga
    15241 tgcgaaaata ttttcttgaa cctgttcact gacagttgga ttacctgaag ctactcggct
    15301 tgaatagtaa gaaaatgaaa taaaatcaac cgtatagtct cttaacaaag caagatcttc
    15361 tgctgtcatt tgaattgaaa tacctaaatg ctcaaaatat tttttagcat aattagggta
    15421 tttgccacgc gcttgcacat caataaaaaa ataattttct ctattttttt gcatcgaagc
    15481 ccaataatct tgtggatgac acgtatttgg atagtattga cctgcagcta acatacatcc
    15541 gatttgacta tttggatcaa cttcatgagc aatttttact gcaatagccg aagccactaa
    15601 ctcatggtga gctgcttgat atttaatttg ctcttgattt tcaccatctt caaaatataa
    15661 gccagcgccc ataaaaggag cgtgtaaaat catattgatt tcgttaaagg tcaaccaata
    15721 tttaactaac cccttaaaac gagtaaataa agctcgtaca agacgctcat aaaaaccaat
    15781 cattttacgg ctccgccacc caccgtaatg tttaataagg tagataggac agtcaaaatg
    15841 tgtgatggtt accaaaggtt caataccata cttacgacac tctttaaaaa tattttcata
    15901 aaaccgaaga ccagcttcat ttggatatag ctcatcaccc tttggaaaaa tacgcgtcca
    15961 tgctattgac atacgatagg ttttgaaacc catttctgca agtaatgcaa ggtcttcttt
    16021 ataatggtga taaaaatcta ttgattcttt tgctggataa aagtatcctt cctcaaaatc
    16081 aaacattttc ttttgtcctg aaataattgc aaatcgatct tctccagtag gaacaacatc
    16141 tacatttgct aggccacgtc catcaacatt gtaagctcct tcacattgat tggcagcagt
    16201 tgccccgccc cataaaaaac cttttggaaa tacttgtttt accatcattt acccctcttg
    16261 ttttttctta tcataatatt aaaaaagacc agttactgac attgattcgc caaatactgg
    16321 tactatccgt gacatgaact ttctacttag aaggtctttt cactaattct gtgtatttaa
    16381 atacttaaaa atagtatcta aacactttta aaggtggagt tttatcgttt cagaaatttt
    16441 agcataggct tgatctgtta ggtgcaaacc atcttttgta aaactgctag caagctgacc
    16501 tttttcgtca actaataaat catacaattc aatataatta atgccagaaa ttactgataa
    16561 ggttttgttt aaagctttta ttgcttgatt atttcttacc ttgacacgct ctatatagtc
    16621 atcttcttca ctaacaggta aaacactcag tatattaatc tctgtcatat aagattccgc
    16681 acgaatttct gcaattatat ccgtgatatt agctatgatt tctgactgtg aatgccctaa
    16741 accaatatca tttgttccaa ttaaaataaa tactttggat ggtaatagtt cccatacttg
    16801 ggttctcaag ttttctaaaa gccaataaga atctgatcct gctactcctc tattaactaa
    16861 aggatagtca cgtcctaaat gtttttttaa aggaaaaaac tcaacgatag aatctcctgt
    16921 gaaaacaatg gcaccttctt gtactgtctg attgttaatc tcaaaaaact gttctcgttt
    16981 catttgataa tctcttaaag ctttgtcaat aacttctaac atcgttctcc ttcattctta
    17041 aatatttctc taggctttct tgtattataa caaaatttca cctgagaatt gaatagtatg
    17101 cttcgtgaaa agctacgtgg ttctattcat tatcactcat cttgtgaaga aagttctcta
    17161 tcgtttctaa cacaccatct tgatcgttac taactgtttg atatttagca atagctttaa
    17221 tttcttctgg cgcatttgac atagcgtaag aaatatttgc tgctctcaac atttccacat
    17281 cattgccccc atcaccaaaa accatcactt gatcctgttt caatccccac ttttcaagga
    17341 gaatagcgat accatccgct ttattgacat cagctggtaa gacatcgata caaccaaaac
    17401 cactagaggt caatctgata tgatgatttt taaagtgttc actaatatct aacaacatct
    17461 cagaaacaag attttcattt acccaaagtg tcatttgaaa atatcggtca tcatctaagg
    17521 gcagcaaatg gtctttatag ataaaattag gcatatagta ttctaattca gtccgcctat
    17581 taataggagt tcttcgctca
//
LOCUS       DATRME010000039        17240 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRME010000039 DATRME010000000
VERSION     DATRME010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17240
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            43..852
                     /locus_tag="VBC23_001263"
     CDS             43..852
                     /locus_tag="VBC23_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6843210.1"
                     /translation="MALILLFTADFLTYIIIELIISLDFWKLEWDSDIVQLIILLATS
                     ISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRGWLVGSVLFGACLILTIISGSGVI
                     ETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWLLPVSATKYSKIVSVSISSIFFGL
                     LHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWGACGIHGAWNCVQGSVFGIEVSGE
                     PMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVLIVACYWLYQKSNFPYD"
     gene            959..1549
                     /locus_tag="VBC23_001264"
     CDS             959..1549
                     /locus_tag="VBC23_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6843211.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1583..2854
                     /locus_tag="VBC23_001265"
     CDS             1583..2854
                     /locus_tag="VBC23_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6843212.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            2867..3298
                     /locus_tag="VBC23_001266"
     CDS             2867..3298
                     /locus_tag="VBC23_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843213.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3390..4274
                     /gene="truB"
                     /locus_tag="VBC23_001267"
     CDS             3390..4274
                     /gene="truB"
                     /locus_tag="VBC23_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6843214.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4287..5219
                     /locus_tag="VBC23_001268"
     CDS             4287..5219
                     /locus_tag="VBC23_001268"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6843215.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5262..5675
                     /locus_tag="VBC23_001269"
     CDS             5262..5675
                     /locus_tag="VBC23_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6843216.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5668..5949
                     /locus_tag="VBC23_001270"
     CDS             5668..5949
                     /locus_tag="VBC23_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6843217.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            5939..6703
                     /locus_tag="VBC23_001271"
     CDS             5939..6703
                     /locus_tag="VBC23_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6843218.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            6771..8081
                     /locus_tag="VBC23_001272"
     CDS             6771..8081
                     /locus_tag="VBC23_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6843219.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8260..9126
                     /locus_tag="VBC23_001273"
     CDS             8260..9126
                     /locus_tag="VBC23_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6843220.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9172..10089
                     /gene="pstC"
                     /locus_tag="VBC23_001274"
     CDS             9172..10089
                     /gene="pstC"
                     /locus_tag="VBC23_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6843221.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10079..10966
                     /gene="pstA"
                     /locus_tag="VBC23_001275"
     CDS             10079..10966
                     /gene="pstA"
                     /locus_tag="VBC23_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6843222.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            10978..11781
                     /gene="pstB"
                     /locus_tag="VBC23_001276"
     CDS             10978..11781
                     /gene="pstB"
                     /locus_tag="VBC23_001276"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6843223.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            11793..12551
                     /gene="pstB"
                     /locus_tag="VBC23_001277"
     CDS             11793..12551
                     /gene="pstB"
                     /locus_tag="VBC23_001277"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6843224.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12585..13238
                     /gene="phoU"
                     /locus_tag="VBC23_001278"
     CDS             12585..13238
                     /gene="phoU"
                     /locus_tag="VBC23_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6843225.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13384..15933
                     /locus_tag="VBC23_001279"
     CDS             13384..15933
                     /locus_tag="VBC23_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6843226.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16095..16775
                     /locus_tag="VBC23_001280"
     CDS             16095..16775
                     /locus_tag="VBC23_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6843227.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            16759..>17240
                     /locus_tag="VBC23_001281"
     CDS             16759..>17240
                     /locus_tag="VBC23_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6843228.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     "
BASE COUNT         5839 a         2893 c         3194 g         5314 t
ORIGIN      
        1 gcaaaaggta aagtatcaag gcatgtcctt ttaactatat tattggcttt gatattacta
       61 tttacagcag atttccttac ctatattatt attgaattga ttatctcgct tgatttttgg
      121 aaactagagt gggatagcga tatcgtccag ttaatcattt tactagcaac ttcaattagt
      181 acggtaatgt tatttatgtg ggtgaaattt aaagagaagc gtccaattag ttctctaggg
      241 ctatttaaag aaggagccct aacagaatta tctagaggat ggttagtcgg gagcgttctc
      301 tttggagcct gcttaatatt aacaataatt tcaggctctg gagttatcga aactgttcgt
      361 tttacagctg ataaattgat ttggtttctt atttttgctg ttggatggca ggtacaaagc
      421 gcatctgttg aaatattgtg tcgtggatgg ttacttccgg tgagtgccac aaagtatagt
      481 aagatagttt ctgtcagtat cagttcaatc tttttcggtc ttctgcatag cgctaataat
      541 catgtaagcc ttatctcgat tttcaatctg tgtctctttg gactatttct atcgctctat
      601 gttattttaa aaggtaacat ctggggggcg tgtggtattc atggcgcctg gaactgtgta
      661 caagggagtg tctttggaat cgaagttagt ggagagccaa tgttgtctaa ctccctagtc
      721 catgttaaaa cctatggtgc tgactggatt agcggtggaa aatttggtgt ggaaggaagc
      781 atgattacca gtattgttct tatcgtagca tgttattggc tttatcaaaa atcgaatttt
      841 ccttacgact aatagattta atctaaataa gcttctctta ctgaaagctt attttttatt
      901 ttagtattaa aaaactatgt tttgtgatat aatattttaa agacgaaagg agtctattgt
      961 gccacaaaat ccaacaagta acccagagaa aaaaacttat ttattatctg aattggtaga
     1021 ctgctttaaa ggtaaagctg tgccttcaaa agcagaagca ggtgatatcc gtattattaa
     1081 tttgagtgat atgtctcctt taggaattga ctaccataac ttaagaacat tccaagatga
     1141 acaacgatcg cttttaaaat atctacttca ggaaggcgat gttttaatcg cttcaaaagg
     1201 aactgtcaag aaagttgcta tttttgaaga gcaagattac cctgtcgttg cctctgctaa
     1261 tattacaata ctaaggccta cacaacacat tagagggtac tacttaaagc ttttttttga
     1321 ttcagaagaa ggtcaacagg cacttgagaa tgctaacaaa ggaaaagctg tgatgaatat
     1381 tagcacaaag gaactgttaa atattgcaat accaagtatc ccgcttttta gacaggatta
     1441 tcttatccaa cgttataaac aggggctcaa cgattacaaa cgtaagatag cacgtgcaga
     1501 gcaggagtgg gaacgtatcc aaaatgacat cagacaacaa cttagctaga accaaataca
     1561 aaacatcata ggaagagtta tcatgaacga aatcaaatgc cctcattgtg gaacagcttt
     1621 tgccatcaac gagtctgaat accatcaatt actagaacaa attcgtggag atgcttttga
     1681 caaagaagta agtgaacggt tggaaaaaga acgtctaata ttaggggagc aagcaaaaaa
     1741 tcaattacag gaagttgttg tagaaaaaga caaggagata gctaaacttc agtacaaagt
     1801 caaacaattt cttatagaaa aagacaatct tctcaaagac aatgagtacc aactcgctga
     1861 gcaattaaat caaaaagaca tgatgcttcg cgaccttgaa aaccaaatcg atagactacg
     1921 tttagagcat gaaaatagct tgcaagaggc gctaacaaaa gtcgaacgag aaagagatgc
     1981 aatacaaaat cagttgcaca ttcaagaaaa agaaaaagat ttagctttag cttcagtaaa
     2041 aagtgattat gaagtacaac taaaggcagc caatgaacaa gtagaattct ataaaaactt
     2101 caaagctcaa cagtctacta aagcagtagg agaaagttta gaacattatg ctgaaacaga
     2161 atttaataaa gtgcgacatt tggcctttcc taatgcttat tttgagaagg acaatacatt
     2221 atcaagtcgt ggctcaaagg gggactttat ctatcgagaa aaggatgaaa atgaccttga
     2281 gtttttaagt atcatgtttg aaatgaaaaa tgagtctgat gatactatca agaagcataa
     2341 aaatgaagat tttttcaaag aattagataa agatcgtcgt gaaaaatctt gcgaatacgc
     2401 agttttagta actatgcttg aagcagacaa tgactattat aatactggaa ttgttgatgt
     2461 tagtcacaaa taccctaaaa tgtacgttat acgtccacaa ttttttatcc aattaattgg
     2521 tattctaaga aatgcagcac tcaatacctt aaaatataaa caagagcttg ctttgatgaa
     2581 agaacaaaat attgacatca cacattttga agaagattta gatattttca aaaatgcatt
     2641 tgctaaaaat tataattctg caagcaaaaa tttccagaaa gcaatcgatg aaatagataa
     2701 atctattaaa cgtatggaag ctgttaaggc tgctttaaca acgtctgaaa atcaactacg
     2761 tcttgcaaat aataaattag acgatgtttc tgtcaagaaa ttaacaagaa aaaatccaac
     2821 aatgaaagca aaattcgatg ctctaaaaga ctaagaggtt atgattatga agattagaac
     2881 tgctacacta gacgatagtg aaaaattagt ccctttgtat caagaattag gatacgcaat
     2941 atcattatca gagattcaat ctattttaaa ggttattctt actcattcag attatggctt
     3001 tttgatagct gaggataatg gcaaactact cgccttcgtt ggatatcata aactttactt
     3061 ttttgaaaaa agtgggactt actatcgtat tttagcacta gtagttaatg aaaagcaccg
     3121 tcgtaaaggc atcgctagtc aacttattaa tcacgttaaa cagctagcta aaacagatgg
     3181 aagtgaagta ctggctttaa atagcagttt aaaagaatat cgtcaggaag cttatcattt
     3241 ttatgaaaac ctagggttca agaaggttag tacaggcttt tcatactatt tagattaggt
     3301 ataaatcctt atagcactat accaaaagag cgtaagtttt tacgctcctt tctttgtgtt
     3361 accgcttatt tactgatata atataaaaca tgattacagg aataattaat ttaaagaaag
     3421 aagctggaat gacatcacac gatgctgtat tcaagcttcg aaagatatta catacaaaaa
     3481 aaataggaca cggcggaact cttgatccag atgttgttgg ggttttacca attgcggttg
     3541 gaaaagccac tcgtgttatt gaatatatga cagaaagtgg caaaatttat gaaggagaaa
     3601 tcactttagg atatgccaca agtacagaag attctagtgg agaggtcatt tcaagaactc
     3661 cactaactca atccgatctt agcgaggatg ttgtggatca tgcgatgaaa agttttaccg
     3721 gtccaattac acaagttcct cctatgtatt cagcagttaa agtaaatggt aaaaaactct
     3781 atgaatatgc tcgttcagga gaagaagtag agcgacctaa acgacaaata acaatttctg
     3841 aatttagacg cacaagccca ttgtacttcg aaaaaggcat ctgccgtttt agtttctatg
     3901 tctcatgcag taaaggtacc tatgttagga cgttagctgt tgatctagga ataaaactag
     3961 gatatgctag ccatatgtct ttcctaaaaa gaacctcatc tgcaggatta tctattacac
     4021 aatcattaac gcttgaagaa attaatgaaa aatataagca agaagatttt tcatttttac
     4081 ttccaattga atatggtgtt ttagatctcc caaaagttaa cctaactgag gaagataaag
     4141 ttgaaatctc atacggacgt cgtatcttat tagaaaatga agcagatacc ttagcagctt
     4201 tttatgaaaa tagagttatc gctatactag aaaaacgtgg caatgaattt aaaccacaca
     4261 aagtattgtt ataaagaaag tcaatcatga aaatccatta cattaatgat tataaagata
     4321 ttcaagctaa agaagattgc gtcttagttt tgggctattt tgatggctta catctgggac
     4381 ataaagccct ctttgataaa gctaaaaaaa tcgcgaccga aaaaaatttg aaaattgtcg
     4441 tcttaacgtt taatgagaca cctcgtctaa ccttcgctcg cttccaacct gaattattgc
     4501 tacatcttac ctctccagaa aaacgttctg aaaaatttca agagtatggc gtcgatgagt
     4561 tatatttaat gaattttacc agtcattttt caaaagtatc ttctgacctt tttattaaga
     4621 aatatattta tggtctcaga gcaaaagctg ctgttgtcgg ttttgattat aagttcggtc
     4681 ataatcgtac cagtggtgat tatctagcgc gtaatttcaa gggaccagtc tatattattg
     4741 atgaaatttc tgaaggtggt gaaaaaatct cttctacacg tattcgtcaa ttgattacag
     4801 agggtaatgt tgagaaagct aaccaacttt tgggttatga gttttcaacc tgtggcatgg
     4861 tagtacatgg agatgctaga ggacgaacta tagggttccc aactgctaat ctagctccta
     4921 ttaatcgcac ttacttacct gcagacggtg tttatatttc caatgttctg attaatggaa
     4981 aatattatag agcaatgacg agcattggaa aaaatatcac ttttggtggt acagaacttc
     5041 gtttagaagc taatatcttt gattttgatg gcgacattta tggagaaact attgaaattt
     5101 tctggttaaa aagaatcaga gaaatggtaa agtttaatgg tattgacgat cttgtaaaac
     5161 aacttaaaaa agataaagaa attgctttaa attggaaaaa agatagtcaa actctttaat
     5221 tttttgtata atagtctcta gtaaatacaa gaggcggtta tatgattacc ttatttttat
     5281 cacccagctg tactagttgt cgaaaagcgc gtgcttggct aagtaagcat gaggttgctt
     5341 ttgaagagca taacattatt acaagtccac tcaataaaga agaactatta caaattctat
     5401 cgtttacaga aaatggtaca gaagacatta tttccacacg ttcaaaagtc tttcaaaaac
     5461 ttgctattga tgtagacgaa ttatcaacgt cttcattaat ggaattaatt tcagaaaatc
     5521 caagtctttt acgacgccca attattttag acaaaaaacg tatgcagatt ggttttaatg
     5581 aggatgaaat tcgtgccttc ttaccgcgtg actatcgtaa acaagaactg aaacaggcca
     5641 ctattcgtgc tgaaattgag ggaaaacatg attagtagta attatagtta tcctcttgat
     5701 ccttcatgga atacggaaga tattactaag gtacttcgct ttttaaatca agttgaacac
     5761 gcctatgaaa atagtgttaa agttgatgat ttgctagata gctataaaga atttaaaaaa
     5821 tttgttaaat ccaaggcgca ggaaaaacaa attgaccgtg aattccagag aactagcggt
     5881 tattccactt atcaagctgt taaagcagca caacaacaag cgaaaggatt tatttcactt
     5941 ggacgctaaa tttgatttcg ctaaacaatt agtgtataaa gctggtcaat tcattaaatc
     6001 tgagatgcaa aatacttttg atgttgagga aaagtctcga tttgatgatt tagtgacaag
     6061 tcttgataaa aaaacacaaa aattattaat tcaagaaatt attcaacatt acccagatga
     6121 taatatactt gccgaagaag atgatgtgcg ctcacccatt gcacaaggta atgtttgggt
     6181 tttagaccct attgatggaa ctgttaattt tattgttcaa aaagataact ttgctgttat
     6241 gttagcttat tacgaagaag gcgttggaca atttggtatc atctacgacg ttatggctga
     6301 tattctttac agcgggggag gacattttga tgtctatgct aacgataaaa aaattgttcc
     6361 ttttcaagag tgtcctttag aacgttgctt acttggagta aattctgcta tgtatgcaga
     6421 aaatgattgc ggtattgccc atttagcttc tgaaacctta ggtgttcgta tctatggtgg
     6481 tgctggaatt agcatggcaa aagtcatgca gggcaaatta ttagcttatt tttcgtatat
     6541 tcaaccttgg gattatgctg cagcaaaaat catgggggag acattaggat ttaccttatt
     6601 aacgcttgac ggggaagaac ccaactattc aacaagacaa aaagtaatgt tcctaccaaa
     6661 gtcgaaatta aatcttatcc aatcctacct taccaaaaaa taaacttaaa accttctaat
     6721 taaatagaag gtttttttgc acgcccgtga ttatgtagta taatagaaac atgaaattac
     6781 cgaatgaatt tattgaaaaa tatcagacta tacttaaaga tgaagcagaa gctttttttg
     6841 acagttttga acaaaaaccc atatcggcat atcgcaccaa tcctctcaaa gaaaagcaac
     6901 ttgattttcc aaatgccatc ccaagtaccc cttgggggca ttatgggaaa atatcaggaa
     6961 aatcaataga acacacaact ggccttgttt attctcaaga acctgctgct cagatagtgg
     7021 ctcagattgc tgaaccacaa gaaggaatga aagtgctaga tttagcagct gcacctggtg
     7081 gaaaaacaac acaccttcta tcatatttaa acaatactgg tctattagtt agtaatgaaa
     7141 tttcaaataa acgtagtaaa attttagtcg aaaatgtcga acgttttggt gctagaaatg
     7201 ttatcgtgac taatgaaagc tcccaacgac tagccaaatg ttttaattct ttttttgatc
     7261 ttattgtttt tgacggtccg tgttctggtg agggaatgtt ccgcaaagat cctcaagcaa
     7321 tacaatattg gcacaaagat taccccactg aatgtgcaca gttacaaaga gatattttaa
     7381 aagaagcaat caaaatgcta gctcatggtg gtatacttgt atactctact tgtacatggt
     7441 caccagaaga gaacgaagaa gtggttaatt ggctccttca agagtatgat tacttagaac
     7501 ttgttgatat tcctaaacta aatgggatgg ttgaagggat taatgtacca caagttgcaa
     7561 ggatgtaccc tcatcatttt caaggtgaag gacaattcgt tgcaaaactg agagatacta
     7621 gatctaagga agcacagaaa attaagccaa aagcacagaa aataaataaa atgcagttac
     7681 aattgtggca acaatttgca caagaccatt taaagataga cttgaacggt gttttagatg
     7741 ttttcggcga ccaactctat cttctaccta atggtctccc tgatttgtct aaattaaaaa
     7801 tagcacgcaa tggtctccat ctaggcactt tcaagaagaa tcgttttgag ccatcgtttg
     7861 ctttaggaat ggcacttagc gaacatgacc tagtacagtc tattgaaatt gatatagaac
     7921 agtttgaggt gtacgtatct ggaaatgtag tcaaactaga caagactgtt ccaaacggtt
     7981 ggtatcaaat tcttgtaaaa ggcaatggat taggttttgc aaaagtgaca aataatactc
     8041 ttaaaaatta ttaccctaaa ggactacgat ttcagacata actgttattg aatactttta
     8101 ataaaaaagg aaaaagaaat acttttcaaa ccctagaaac ttatggtact atgggacagt
     8161 gatgtgtgtt tttactattt gtaaatcatt aaacactcaa tgattttagt aatatatatc
     8221 aactactaaa tatcaaaatt tattcaaaag aataggaaaa tgaaaatgaa aaaaaacatt
     8281 tttttgttaa ccttgctagc caccgtctta ttgacgctgt ctggttgtgc taattggatt
     8341 gataaaggtc aatcaatcac cagcgttggt tcaaccgcct tacagccact tgtagaagct
     8401 gcagcagatg agtttggtaa gacaaacctc ggtaagacta tcaatgtcca aggaggaggc
     8461 tcaggaacag gcttatcaca agtacagtct ggagctgttc aaattggtaa tagcgactta
     8521 tttgcagaag aaaaagatgg tattgatgct aagaaattga aagaccatca agtcgcagta
     8581 gctggattag ctgttattgt taataaaaaa gttaatgtta aaaatctaac cacccaccaa
     8641 cttcgtgata tttttgcagg aaaaatcaag aattggaaag aagtgggagg acaagattta
     8701 gatatttcca tcatcaatcg agcagcaagt tctggatccc gtgcaacctt cgataatact
     8761 attatgggaa atgttgctcc tattcaaagc caagaacaag attcaaatgg tatggtgaaa
     8821 tcaattgttt ctcaaacacc tggtgcgatt tcatacctcg cttttgcata cgttgataaa
     8881 tctgtcggaa cattaaaact aaatggtttt gcgccaacag ctaaaaatgt tactaccgac
     8941 aactggaaac tttggtcata tgaacatatg tatacaaaag gtaatgaaac aggtctaaca
     9001 aaagaattct tagactacat gaaaagtgat aaagttcaaa gtagtatcgt acaacatatg
     9061 ggatatatct ctattaacga catgaaagtt gttaaagatg ctgaaggtaa agtaacccca
     9121 aaataaaact taatcacatc tagataaagc atcttgatcc taggaggaat tatgaaaaat
     9181 caagaattag ctaaaaaatt aacatctcct tcaaaaaact ctcgattaga aaaatttggt
     9241 aaaggaataa cttttctttc gcttgcccta atcgtcttca ttgttgcgat gattttgatt
     9301 tttgttgctc aaaaaggact atctacattt tttgttgatg gcgttaaatt aacagacttc
     9361 ttatttaata caaaatggga accaagtgct aagtcattcg gtgctcttcc aatgatagct
     9421 ggatcattta ttgttactat tctatcagct atcattgcta caccatttgc tatcggtgct
     9481 gccgtattta tgactgaaat ctcacctaaa tatggttcta aaattttaca acctgcagtt
     9541 gaattactag ttgggattcc ttctgttgtt tacggtttta ttggtttaca gattattgtt
     9601 cccttcgttc gctctatttt tgggggaaca ggttttggca ttttatctgg ggtctgtgtc
     9661 ctcttcgtca tgattctacc aactgttact ttcatgacag tagatagcct tagagctgtg
     9721 ccacgtcact ataaagaagc aagtttagcc atgggggcaa ctcgttggca aacaatttgg
     9781 cgtgttatat taaacgctgc acgtccagga attttcactg caattgtttt tggtatggca
     9841 cgtgcatttg gtgaagctct agcaattcag atggttgtcg gaaactctgc aattttacca
     9901 acatcattga caacaccagc agcaacatta acgtctgttc taacaatggg tattggtaat
     9961 actgtaatgg gaactgtaca aaacaatgtc ctctggtcgc tagccttagt acttttaatt
    10021 atgagtttag catttaatac agttattaag ttaattacaa gggaaggaaa gaaaaactat
    10081 gaacgctaaa aaggctgaca aattagcaac tacaatactc tatagtattg cagctatcat
    10141 cgttacaatc ctcgcctctc ttttaatttt tattcttgtt cgcggattgc cacacgtatc
    10201 ttggtcattt ttaactggga aatcatcatc atatgaagca ggaggcggta ttggaataca
    10261 attatacaat tcctttttcc tacttattgt tactctgatt atttctattc ctctatcttt
    10321 aggagctgga atatacctct cagaatatgc taaaaaagga cgtttaacaa actttgttag
    10381 aacatgtatc gaaattttgt catctcttcc ttctgttgta gttggtctgt ttggttacct
    10441 aatcttcgtt gttcaatttc aatatggctt ctcaattatc tcaggtgcct tagcacttac
    10501 tgtttttaac ttaccccaaa tgacacgtag tgttgaagat agccttcaaa atgtacacca
    10561 cacacagcgt gaagcaggat tggccctagg tatttctcgt tgggaaacgg tcatttacgt
    10621 cgttgtccct gaagctctgc caagtattgt aactggtgtt gtgctagctt caggacgtat
    10681 tttcggtgag gcagcagcac ttatttatac agctggtcaa tctgctccag cacttgattg
    10741 gtcaaattgg aacgtcctta gtgttacaag tccaatctca attttccgtc aagcagaaac
    10801 tttagctgtt catatttgga aagttaatag cgaagggaca atccctgatg caacacaagt
    10861 atctgccggt agcgccgcag tcttattggt tgttattctt attttcaacc tttcagctcg
    10921 ctcgatcggt aagaaacttc attcgaaatt aacctcgtct aattaaaagg agtagtaatg
    10981 gcagaatata attgggacga acgccatatc attaccttcc ctgaagaaaa ttcagctctc
    11041 accacaaaag atttacacgt ttactacggt gagaaagaag ctatcaaggg aattgatatg
    11101 caatttgaaa aaaataaaat tacagcctta atcggcccat ctggatgtgg aaaatcaaca
    11161 tatcttcgta gcttaaatcg tatgaacgat acgattgata ttgcacgcgt gactggacaa
    11221 atcatgtatg aaggaattga tgttaacgct caagatatca atgtttacga gatgcgaaaa
    11281 catatcggaa tggttttcca acgtccaaac ccgtttgcaa aatcaattta taaaaacatt
    11341 acctttgcat atgagcgtgc tggtgtaaaa gataaaaaat tcttagatga agttgtcgaa
    11401 acctcactaa aacaagctgc tctatgggat caagtaaaag atgaccttca taaatcagca
    11461 ttcactttat ctggcggtca acagcagcga ttatgtattg cacgtgctat cgcagttaaa
    11521 ccagaaattc tcttaatgga tgaacctgct tcagcactcg atccgattgc aactatgcaa
    11581 ctagaagaga caatgtttga actcaagaaa aattacacta taattatcgt aacacataat
    11641 atgcaacaag cagcacgtgc tagtgattat actgcatttt tctatcttgg agacttaatc
    11701 gaatacgata aaacgaataa cattttccaa aatgctaaat gtcaatctac tagcgattat
    11761 gtttctggtc gatttggata gtgaggtaaa agatgacaca acctatttta caagtcagtg
    11821 atttgtctgt ttactataat aagaaaaaag ctttaaaaga agtctcaatg gacttctatc
    11881 ctaatgaaat tacagcctta attggtccgt ctggttctgg aaagtcaaca ctcttacgag
    11941 ctattaaccg tatgggagac ttaaatcctg aagtcactct aacaggagca gtgatgtata
    12001 acggacataa tgtttatagc ccgcgcactg ataccgtaga acttcgtaaa gaaattggga
    12061 tggtttttca gcagcctaat ccatttccta tgtcagtatt tgaaaatgtt gtttatggtc
    12121 ttagactcaa aggaataaaa gacaaagcta cgttagatga agccgtagag acttcattga
    12181 agggcgcgtc tatctgggac gaggtcaaag atagattaca cgactctgct cttggtttat
    12241 ctggtggtca acaacaacgt gtttgtattg ccagaaccct tgctaccaag cctaaaatta
    12301 tccttctaga tgagccaacc tctgcacttg acccaatttc tgctggtaaa attgaagaga
    12361 ctttacatgg tctaaaagat caatatacca tgttacttgt tacacgttct atgcagcaag
    12421 cctcacgtat ttctgatcgt acaggtttct tcttagacgg taacttaatt gaatatggca
    12481 atacaaaaga aatgtttatg aatcctaaac ataaagaaac tgaagattac attacaggta
    12541 aattcggtta ataccatata ataagatcag aaagagaata caatatgtta agatcaaaat
    12601 ttgatgaaga attagataaa ctgcataacc aattctatgc aatgggaatt gaagctatcg
    12661 gacaaatcaa aaaaacagtt cgtgcttttg tcagtcatga tcgagagtta gctaaagaag
    12721 tgatagaaga tgatgtaacg cttaacaact ttgaaacgaa gttagagaaa aaatcattgg
    12781 aaattattgc actccagcag cctgtatcac aagacttacg caccgttatt accgttttaa
    12841 aagctactag tgatgttgag cgtatgggag accatgcagc agcagttgca aaagcaacca
    12901 tccgtatgaa gggcgaggaa agaatcccag cagtagaatt agaaatcaat aatatgggaa
    12961 aagcagttaa aaacatgctt gaagaagcct taacagctta tatcaatggt gatgatgaaa
    13021 aagcttacga agtagcagca atggatgaaa tagttgatga ttacttccgt gatatccaaa
    13081 aaatggtagt tgaaacaatt caaaagcatc ctgacgtagc atttgccgcc aaagaatact
    13141 tccaagtatt gatgcacttg gagcgaattg gagattatgg caaaaatatc tgtgaatgga
    13201 ttgtttattt aaaaactgga aaaattatcg agttatgata taattattgc ttgtagagtg
    13261 gtaattgtaa gtatagttcg tgcgatagaa aagctgtaac tatcttgtta cagcttttct
    13321 atcttttcta gcccatacag gactagtaat gtttcaaatg tttctgaatt agttaggagt
    13381 tatatgaaaa ctgtagaaca ttttgttacc aaatttgtcc ctgaaaacta taaccttttc
    13441 ttggatatta atcgtcaaac taaaaccttt tcaggtaacg ttgctgtgtc tggcgaagcc
    13501 ttagataaca atatctcttt tcaccaaaaa gggttaacca ttaaatctgt cttattagat
    13561 aatcaaccat tagatttcca actagatgaa gacaatgaag ctatgcatat tcaattgcat
    13621 gaaactggta gcatggtatt agtctttgaa ttcagcggtc acattaccga taatatgaca
    13681 ggtatgtatc catcttacta tacagttaat ggcatcaaaa aagaggttat ctccacacaa
    13741 tttgaaagtc attttgctcg tgaagtattc ccaagtattg acgaaccaga agcaaaagca
    13801 acctttgatt tgagcctaaa attcgatcaa aaagaaggag agattgctct ttcaaatatg
    13861 ccggaaatca atgctgaaca acgtcaagaa acaggtttat ggacatttga caccacacct
    13921 aaaatgtcct catatttatt agcttttgcc ttgggggaat tgcatggtaa aacaacacat
    13981 accaaaaacg gcactcttgt tggctcatat gccacaaaag cccatcagtt aaatgaatta
    14041 gatttttctt tagatatcgt tgtgcgtgtt attgagtttt atgaggacta ctttggtgta
    14101 cgttatccta ttcctcagtc actccacgtt gctctacctg atttttctgc tggagctatg
    14161 gaaaattggg gattagtaac atatcgtgaa gtctatcttt tagtagatga gaactctagt
    14221 gtttccagcc gtcagcaagt agcattagtt gtagcacatg aaatagctca ccagtggttt
    14281 ggtaacctcg ttactatgaa atggtgggat gatttatggt taaatgaatc atttgctaat
    14341 atgatggaat atgtatcaat tgattatatt gaaccaaaac ttaatatatt tgaagacttt
    14401 caaacaggtg gtctaccatt agctcttaag cgtgatgcca cagatggcgt ccaatctgtt
    14461 cacgttgagg tcaatcatcc tgatgaaatt aacacacttt ttgatccagc tatcgtttat
    14521 gcaaaaggta gccgcctcat gcacatgtta cgtcgctggt taggcgatac agattttgcc
    14581 gcaggactaa aaatttattt tgaaaaacat caatatcaaa atactattgg tcgtgacctt
    14641 tggaatgcac tttctcaaac ctcaggaaaa gatgttgcag ctttcatgga ttcttggtta
    14701 gagcaaccag gataccctgt gatggcagcc aaaatagaag aagatgagct cattttaact
    14761 caaaaacaat tctttattgg tgaacacgaa gataagtctc gcttgtggca aattcctctt
    14821 aatagtaatt gggaaggaat cccagagata ttaaccgagg aaacggtagt aatccccaat
    14881 tttagtcagt tagctgaaaa gaacaaagaa aatggtgcac tgcgtttcaa tactgaaaat
    14941 actgcacatt acattactaa ctatcaaggt caactcttag agcatatcat ttctgatctt
    15001 cctcttatgg ataacatttc taaactacaa attgtacaag aacgtcattt acttgctgaa
    15061 agtggtatga tttcatatag tagtttaata cctttagtct ctcttttgag ccaagagaca
    15121 tcatatcttg ttaactctgc tattaagagt gttattgatg gtttatcctt attcgtccag
    15181 gaagatagcc aagatgaatt cgactttaaa gaatttgtta ataaattatc agcatttaat
    15241 tttaatagac ttggctttga aaaaagagaa ggagaaggag atgatagcga aatggtccgt
    15301 catctatccc taagccttgc tctctactca gataatgaac atgctattga agaagctcac
    15361 catattttta aagcccacga aaataatatc gctgctatac cagctgcaat tcgcctgtta
    15421 gttcttacca atgaaatgaa gcatttcgaa tccaaagaac ttagccactt gttattagag
    15481 acttatagta ctactaccga tgggaatttc aaacgacaac tagcgagtgc tttatctcat
    15541 actacagata gcaagacact caagaaatta ttgtcagatt ggaaaaataa agatattgtg
    15601 aaaccacaag acttagctat gagttggtat gctactttcc ttaaaaattc ttttacacaa
    15661 gaaagtgtct gggagtgggc ccaagaaaac tgggaatgga tcaaagcaac tctaggtggt
    15721 gatatgagtt ttgataaatt tgtgatttat ccctcaagta gtttcaaaac agaagaacgc
    15781 cttgaacaat ataaaaactt tttcgagcct caattaagcg atatggcgat tagccgtaat
    15841 atttcaatgg gaattaaaga aatttctgct cgtgtccttt taatcacgaa gcaaaaagaa
    15901 gaagttatca atactatcaa aaaatatatc taataaactc aaatagagat tgatggatta
    15961 ttcaatctct atttctttgt ctgtgtcatg aaattagttg acataaaaca tactttattt
    16021 aggatttatt aagcaaagtt tggtattcta atattaatag ttcagataaa atatgtacat
    16081 cgaaaggata tactatgata aaaattttat taattgaaga tgacttgagt ctttccaatt
    16141 ccgtttttga tttccttgat gactttgccg atgtcatgca aatttttgat ggagaagagg
    16201 gtctttacga agccgaaagt ggagtctatg atctgattct tctagatctc atgttacctg
    16261 aaaaaaacgg cttccaagtg ttgaaagagc tacgtgaaaa aggtattaca acccctgtat
    16321 taataatgac cgctaaagaa agcattgatg ataagggaca aggtttcgac ttaggtgctg
    16381 atgattatct aacaaaacct ttttacctag aagaactaaa aatgcgaatt caggctcttt
    16441 taaagcgttc tggaaaattt aatgataaca gcctaattta tggtgatatt cgcgtagaca
    16501 tgtctactaa ctcaaccttt gttaatcaaa cagaagttga gctcttaggt aaagagtttg
    16561 atcttttagt ctatttcctt caaaatcaaa atgttatctt accgaaatct caaatctttg
    16621 atcgtatttg gggatttgat agtgatacaa cgatttcagt tgtagaagtc tatgtctcta
    16681 aggttcggaa aaaattaaaa ggaacacttt tttcagaaaa ccttcaaact ttgcgtagtg
    16741 taggatatat tttaaagcat gtcgaaaccg attaagataa gtaaattcaa aaaaaatgtc
    16801 tcagatagtc attttatcca ttttttcaca gtttttagtg gtatttttct ggttatgact
    16861 gttatcatcc tccaagtcat gcgctatggt gtttattctt ctgttgatag tagtttaaag
    16921 tatatcagta cccatcctaa aaattacatc aatatggtta tgtcacgtac tgctgcctac
    16981 ctagataact ccaatatcgc aagtgtcaag ttaaaacccg gcggtcagac agtagctaat
    17041 actgatatta ttctttttac atcagaagaa gaagtcatta actattttga cgcattttca
    17101 aactatcagt ttttaaaacc taataaaaaa aatctaggtg gcattagtga attaacttta
    17161 accaacatct ttggtcaaga tgagacatat catgctgtca ccgtaaaagt taataaccct
    17221 gcttatccta atgtgactta
//
LOCUS       DATRME010000040        17166 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRME010000040 DATRME010000000
VERSION     DATRME010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..247
                     /locus_tag="VBC23_001282"
     CDS             <1..247
                     /locus_tag="VBC23_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6843229.1"
                     /translation="QKRQFNKMPYEEISEVSHYVKSKGLVLDDYIAYRDTLTKVEETL
                     SDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            complement(426..1523)
                     /locus_tag="VBC23_001283"
     CDS             complement(426..1523)
                     /locus_tag="VBC23_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6843230.1"
                     /translation="MIEKYTKKDGTTAYRLRAYLGVDPMTGKQVRTTRQGFKTEREAK
                     RAEVKLIDDFQRQGAWKSNDKTTFDDVAKLWFEQYRNTVKPSTFLVNQNYYKTILKPH
                     LGQLQMTKITVMICQKFVNCLSRYSGYRLYLSLANRIFKFAVNLGIIDNNPMSKTLRS
                     KCTYKNMDTLTKKYYTKEELNAFLRIVEAEETLEMRLIYRLLSYGGFRIGELIALKDT
                     DFDFRNNTISITKTIAYTKEGWAVQSPKTKKSNRTISMDAETMTLAKLYIKQSIKPLH
                     GSFKLFNFASDTVRKRLDRFILKHGLKRITPHGFRHTHASLLFEAGIPAKIAQERLGH
                     AKIAITMDLYTHLSKKSKDNVADKLAELVAI"
     gene            complement(1696..2310)
                     /locus_tag="VBC23_001284"
     CDS             complement(1696..2310)
                     /locus_tag="VBC23_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843231.1"
                     /translation="MKKPFYKKWYFWTHAILLLVLGFSFFVIYRLAETNIVNEKKIAK
                     LEKTQENKTTSGIRKTISDFTSRFDEELSVRAIKFYLNKDQVVSSFGDEVKLGGGYLT
                     INKPNNDKTRMLATTTDFKNKIIVPIEFKNTTGETKGFDTRDIFAYNGDETISFDSVI
                     SENLDNDGYSVVVKDGETAAASIVFGTNSKIKDIKVRYNSGLWK"
     gene            complement(2442..3221)
                     /locus_tag="VBC23_001285"
     CDS             complement(2442..3221)
                     /locus_tag="VBC23_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843232.1"
                     /translation="MSSKKLENKEIFSKNLAFYMERKRVDRNTLCADLGLKYTTVRDW
                     LKGITYPRIGKIELLAEYFSINKSDLIEEHSTNGATSKVNFDPRQAILLSNYSKLNNV
                     RKNKLLAISETLLSEEQGKVINLQEKMAEYGSRKRVSLSVPGKVSAGTGYWQEDDYDT
                     EVDFYADEIPDEKNYDTVAVVVGHSMEPKIKNGDFLFIKLADQVDINKIGIFQVNGEN
                     YVKKLKNDYLQSLNPKYADITPAEGDDFRTIGEVVDIYREG"
     gene            3582..3731
                     /locus_tag="VBC23_001286"
     CDS             3582..3731
                     /locus_tag="VBC23_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843233.1"
                     /translation="MSEQNEKFIGVLKDMHMQILRDFVNAEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(3720..3932)
                     /locus_tag="VBC23_001287"
     CDS             complement(3720..3932)
                     /locus_tag="VBC23_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843234.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSLEAIKQHNLPF
                     IEAEKQEQINNGKVIAEALERGESLF"
     gene            3991..4149
                     /locus_tag="VBC23_001288"
     CDS             3991..4149
                     /locus_tag="VBC23_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843235.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGLLPALLVLGGITW
                     LIAWFITK"
     gene            complement(4247..4888)
                     /locus_tag="VBC23_001289"
     CDS             complement(4247..4888)
                     /locus_tag="VBC23_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843236.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            4946..5149
                     /locus_tag="VBC23_001290"
     CDS             4946..5149
                     /locus_tag="VBC23_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843237.1"
                     /translation="MEEILSSIAKSLESIATEFKAQNSYREEMKQNMEQMEKIILDIQ
                     SDPFGLKHLKDKALSEKASKQKG"
     gene            complement(5133..5492)
                     /locus_tag="VBC23_001291"
     CDS             complement(5133..5492)
                     /locus_tag="VBC23_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843238.1"
                     /translation="MDKLTNDAKFLLSSMFVKYNERRKDKISKEKSRNFEDIQFIKEN
                     IMNEWSEEDVLDTCFELRKHGYISATAASDTLYLISLTTEAIAELEKQDQAKTLSGRI
                     EYWLEFAKKIKDAIPFA"
     gene            5565..5771
                     /locus_tag="VBC23_001292"
     CDS             5565..5771
                     /locus_tag="VBC23_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843239.1"
                     /translation="MTKMTLKALRATKNWSQEEAARALKVSKDTWGNWERGNTEPSVT
                     KAYQIAYIFDISLDDIIFLPNIAV"
     gene            5825..6784
                     /locus_tag="VBC23_001293"
     CDS             5825..6784
                     /locus_tag="VBC23_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843240.1"
                     /translation="MQEITTQNDFDYSLVDFETQEFLQERSNIIYGIQSKSAYEIGKQ
                     LVKAQEVLAKNRYGCFEEWYSSLGFRKTKAYEYINHYNFIRSQSEQLNIETFEELPKK
                     LQSEMSKPSANPELNQKVFDGDITTHKQYKELERQLKLAEADKERLKQQNERLAEQAL
                     SAKVVEKEVVIEKVPDDYESTKQLNRTLLDKNKELSTTLEEAEWELDSKKLELSTIKL
                     ESQRAIEVTNQIRHLEGKKEKLENLVTSISELSSIISDVQNFFDTKMAPLRFKPIINN
                     VNAHYSVTEVTKMVNTVQTWCDEMYKIIPSGNRKTVEEVIINE"
     gene            6777..7298
                     /locus_tag="VBC23_001294"
     CDS             6777..7298
                     /locus_tag="VBC23_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843241.1"
                     /translation="MSKELSEMNEKDALEVVKGMTIEDMMIEVLSSQKQVKASQEAIK
                     KDVEEVRSLAIEIDKKVHIDDVEASEIKSIISRQSYEFAKEYFDNRKVVPSANLFASK
                     KGQFIRVQHSRLKHHFNVTKYTHIKHTEAEKAISYLKSLSYGLFTPFETRETPKQKEI
                     IELENKNNGDDVA"
     gene            7317..7508
                     /locus_tag="VBC23_001295"
     CDS             7317..7508
                     /locus_tag="VBC23_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843242.1"
                     /translation="MNEKITDEQLNKYLEIRTELGEENFKFINACYDEVLHLKKEEAT
                     KNIKFEITDFSKFRDFAKK"
     gene            complement(7536..7712)
                     /locus_tag="VBC23_001296"
     CDS             complement(7536..7712)
                     /locus_tag="VBC23_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843243.1"
                     /translation="MISSQRIKETLDRHDVSESDKLSVALAEILNKHLSGKNLSETVH
                     EHDKRMARMRGEIM"
     gene            7827..8003
                     /locus_tag="VBC23_001297"
     CDS             7827..8003
                     /locus_tag="VBC23_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843244.1"
                     /translation="MKKKLNKILIDKGMSKKELSEKTGISYNTIMNIGKRDISFNKMK
                     KIADVLDISLNEFR"
     gene            8082..8339
                     /locus_tag="VBC23_001298"
     CDS             8082..8339
                     /locus_tag="VBC23_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843245.1"
                     /translation="MQSQLTYDLLKKQIAEELFDEFKELIQEKDLANRWVNQTALVNE
                     YGYSWQTIKRMESYGLKSFKNGKDKMYCLADVNEIKHLMKQ"
     gene            8369..8512
                     /locus_tag="VBC23_001299"
     CDS             8369..8512
                     /locus_tag="VBC23_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843246.1"
                     /translation="MKLFNFIFAKPKKQEEKPKWTIETHGWEANARRYDNMIEHNNRG
                     RTC"
     gene            8588..8788
                     /locus_tag="VBC23_001300"
     CDS             8588..8788
                     /locus_tag="VBC23_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843247.1"
                     /translation="MNNDWKKRYINNQIDFFNDIKTCVKERWPNMKGNDVDKISIAIY
                     RNWPVSDINSTLEVIETDLEKL"
     gene            8785..9075
                     /locus_tag="VBC23_001301"
     CDS             8785..9075
                     /locus_tag="VBC23_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843248.1"
                     /translation="MKCYVNKQKKLAIDMNYKDKFGKFSSDSIQILEGKLTDSIQIDV
                     ENAMKEIIDKYSQLFDTPIIDDLFTEKEKQLKQSYDVETTLTEMFEVEYEDN"
     gene            9062..9745
                     /locus_tag="VBC23_001302"
     CDS             9062..9745
                     /locus_tag="VBC23_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6843249.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLINYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPINIFVTAWESTQDLTLESGQMITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLEGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            9781..11184
                     /locus_tag="VBC23_001303"
     CDS             9781..11184
                     /locus_tag="VBC23_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6843250.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGYVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QMIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKSMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECPVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            11189..11671
                     /locus_tag="VBC23_001304"
     CDS             11189..11671
                     /locus_tag="VBC23_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEO6843251.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNDRNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPESHHIWKEKTTGFTQAEIEEDDLPF
                     "
     gene            11689..13242
                     /locus_tag="VBC23_001305"
     CDS             11689..13242
                     /locus_tag="VBC23_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEO6843252.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGTYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSIVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESVFRK
                     YWLAFTDRDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHAVSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
     gene            13511..14380
                     /locus_tag="VBC23_001306"
     CDS             13511..14380
                     /locus_tag="VBC23_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEO6843253.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDDINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEVFKPKQEGITTYFGCSVTYTSNGSHEGR
                     EAYERVATGTSYNRNDDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            14334..14681
                     /locus_tag="VBC23_001307"
     CDS             14334..14681
                     /locus_tag="VBC23_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6843254.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGYMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKNFMEVMASKGALAGVAR
                     SVEDALKIVNGYH"
     gene            14760..15047
                     /locus_tag="VBC23_001308"
     CDS             14760..15047
                     /locus_tag="VBC23_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO6843255.1"
                     /translation="MTDKINAETMQVAYNENYQTFLAKNADYGNSFEKSLNDFGFVAG
                     VVRISDKYNRLYNLISSDKNVSESLSDTLNDMANYCVMLAVWLEVKDDTKI"
     gene            15031..15438
                     /locus_tag="VBC23_001309"
     CDS             15031..15438
                     /locus_tag="VBC23_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843256.1"
                     /translation="MIPKFRVWDKVTQAMGVVEAIDYVDNKIYTFYRKVVRRYIPFEE
                     AVLMQSTGLKDKNGIEVFDGDIVKCCRLFNDSLSEYVGQVKFVDFGWNIVDKADTHDP
                     FYNYKDGCPDEIREIENIGNIHENPELLERLEG"
     gene            15460..15537
                     /locus_tag="VBC23_001310"
                     /pseudo
     CDS             15460..15537
                     /locus_tag="VBC23_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            15562..16047
                     /locus_tag="VBC23_001311"
     CDS             15562..16047
                     /locus_tag="VBC23_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843257.1"
                     /translation="MSDIKILDACCGSRLFWFDKNEEHTTFMDIRQETFDIHGKHVNV
                     KPDVLGDFRNMPFENNIFNLVVFDPPHLKYVGQKSIMKAQYGQLDKDNWKEDISKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKEVLSAIPFTPLFGQRRGTTHWMTFVKFAELTGNG
                     G"
     gene            16050..16373
                     /locus_tag="VBC23_001312"
     CDS             16050..16373
                     /locus_tag="VBC23_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843258.1"
                     /translation="MSLIDEVKECGSESHKKWFDRFFDDLKIEEKLKQSAMKGFSGYK
                     IAIPKDDEYLARRLDSQKTIDLLKQRLGDGFDVEITNLESSYKIFGKPVVLSRDLCIY
                     WTNGG"
     gene            16375..16539
                     /locus_tag="VBC23_001313"
     CDS             16375..16539
                     /locus_tag="VBC23_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843259.1"
                     /translation="MENYLCYWEKRGQNWYFLANCEDSPTGRIFNLTGVKFNYCPFCG
                     RRLKEVTNES"
     gene            16529..16693
                     /locus_tag="VBC23_001314"
     CDS             16529..16693
                     /locus_tag="VBC23_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843260.1"
                     /translation="MKVKDLHKVLAKVDPELEVWINEKDEGVLTKAKNVMTWNKHPDK
                     FFINAETYPE"
     gene            16708..>17166
                     /locus_tag="VBC23_001315"
     CDS             16708..>17166
                     /locus_tag="VBC23_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO6843261.1"
                     /translation="MNIEEAKKIVDKLSVDSDELWKIPMIPAHKVKALLDTLDQPKPE
                     VPQMIFDVIKSFDDDVDYLHQHMSRQSDEVREWLTHNECEFYEAWLAYPNITIEKEKL
                     YTVEIPNPNSDLKIILVKVNKKLKLIEAYEDQLEEYKNIRNVTESEIRKDF"
BASE COUNT         6142 a         2760 c         3210 g         5054 t
ORIGIN      
        1 tcaaaagcga caatttaata aaatgcctta tgaagaaatc agtgaagtta gtcattatgt
       61 taagagtaaa ggactagttt tagatgacta tattgcttat cgtgatactt taacaaaagt
      121 ggaagaaact ttgtcagata ttgataaaga aaaatttgaa aaactgatta atggtgaacg
      181 ttttgcgggg aagaaaaaat tcatacgaaa tattcaacct tttttcaatg cttttaaagc
      241 ggattaatcg attttatttc tgcagtagat agctgcagaa tttttttata gggaaagttc
      301 gctgatatat tcctgtgaaa gcctttgttt tttgttataa tataagatta cctctcagaa
      361 tcattgatat caaggctttt ttaaagtcta aaaacagact tactacgttc cccactacgt
      421 ttgtgttaaa tagcgacgag ttcggccaat ttgtcagcaa cattatcctt tgattttttg
      481 gataagtgag tatataaatc catcgtgatt gctattttag cgtggcctaa ccgctcttgt
      541 gcaatcttag cgggaatccc agcctcaaac aacaacgaag cgtgggtatg tctaaacccg
      601 tggggagtaa tcctttttaa tccatgcttc aatataaatc tgtccagtct ttttctcacg
      661 gtgtcgctag caaaattaaa caatttaaac gatccgtgta aaggtttgat actttgctta
      721 atatataatt tggctaacgt catggtctca gcgtccattg atatggtgcg attgcttttt
      781 ttggttttag gagattgtac agcccatcct tcttttgtat aagcaatggt ttttgtaatg
      841 ctgatagtat tgttgcggaa atcaaagtcg gtatctttta aagctattaa ttcaccaatc
      901 ctaaaaccgc cataactcag caatctataa atcagacgca tctctagagt ttcttcagct
      961 tccacaatcc tcaagaaagc attcaattcc tcttttgtgt aatatttttt ggtgagtgta
     1021 tccatgtttt tgtaagtgca ctttgatctc aacgtcttgc tcatggggtt attatcaata
     1081 atacctaagt tgacagcaaa tttaaaaatt ctgtttgcta aacttagata aagcctataa
     1141 ccgctatatc gagataggca attcacaaat ttttgacaaa tcatgacagt tatcttcgtc
     1201 atttgcagtt gtcctaaatg tggctttaaa attgttttat agtagttttg gttaaccaga
     1261 aatgttgacg gtttgactgt atttcggtac tgctcaaacc acagtttggc tacatcgtca
     1321 aatgtagttt tatcgttgct tttccaagcg ccttgacgct gaaaatcatc aataagtttt
     1381 acctcggctc ttttagcttc tctttctgtt ttaaaccctt gcctagttgt cctgacttgt
     1441 ttaccagtca tgggatcaac accaaggtat gctcttaagc gataggcagt tgtgccatct
     1501 tttttagtgt atttttcaat cattgttttt tcctctctct ttgcgctggg gagtgctgat
     1561 tttgagatag gatattggca tcacctccta aaaatgatat aattaggtac aagaaaacga
     1621 cctttttaat ggttgtttct tatactagat tcgccccacg ctcagaccct cccaaatttg
     1681 agcgtggggc tttttttatt tccataatcc tgagttatat ctcactttga tatctttaat
     1741 cttgctgttt gtcccaaaaa caatactagc cgctgctgtt tctccatctt ttacaacaac
     1801 gctatatccg tcattatcta aattttcgct gataactgaa tcaaaagaaa tagtttcatc
     1861 tccattgtag gcgaaaatat ctcttgtatc aaaacctttt gtttccccag ttgtattttt
     1921 gaattctatc ggcacaatga ttttattttt gaaatctgtt gttgttgcta acattcttgt
     1981 tttgtcatta tttggtttat ttatagttaa gtaacctcca cccaatttaa cttcatcacc
     2041 aaaagacgat acaacttgat ctttattaag ataaaattta atagctctga cagacaattc
     2101 ttcgtcaaaa cgactggtaa aatcggatat ggttttccta atacctgacg tcgttttatt
     2161 ttcttgagtt ttttctaatt ttgctatttt cttctcgtta acaatattag tctctgccaa
     2221 tctgtaaatg acaaaaaacg aaaatcctaa aactaatagt agtatagcgt gtgtccaaaa
     2281 ataccacttt ttataaaaag gttttttcat atcataaatc tcctaaatta aatttttaaa
     2341 ctcatttact accatcgctt catcgcagat ggtgtttaac ctatacgctt tcataaatct
     2401 ataaacatta aaatcttcta tgtcgtcaag ttttccacag actacccttc tctataaata
     2461 tctaccacct caccaatggt tctgaaatca tctccttcag ctggtgtgat gtcagcatac
     2521 tttggattaa gtgactggag gtagtcgttc tttaatttct tgacatagtt ttcaccgtta
     2581 acttggaaaa taccaatttt gttaatgtca acttgatcag ccagcttaat aaataaaaag
     2641 tcgccatttt ttatttttgg ttccattgag tgaccgacaa caaccgcaac ggtgtcatag
     2701 tttttttcat ctggtatttc atcagcgtag aagtcaacct ctgtgtcata gtcatcctct
     2761 tgccaatatc cagtaccagc agacacttta ccgggtacag atagacttac acgttttctg
     2821 gagccgtatt ctgccatctt ctcttgcaag tttatcactt tgccttgttc ctcagataaa
     2881 agtgtctcag atatagccag caacttattc tttcgtacgt tgttaagttt tgaatagtta
     2941 gataagagaa ttgcttgcct tggatcaaag ttgactttgg aagttgctcc attagtagag
     3001 tgttcttcaa tcaaatctga cttgttaatt gaaaagtatt cagccaaaag ttctattttc
     3061 cctattcgtg gataagttat acccttgagc caatctctaa ccgtagtata cttcagtcct
     3121 aagtctgcac atagtgtatt tctgtctact ctttttcgct ccatataaaa tgcaaggttt
     3181 ttagaaaata tctctttgtt ctctaatttt tttgaagaca tttttgcacc tccttataat
     3241 gtatatatta cggcaaaaac gcaaaaaagt aaataaaaaa ataaaaaaac cgtaaaaaaa
     3301 cgcaaaaaaa tacttgacat tgcggtttaa ccgcagtata atataatcaa gcttaaggaa
     3361 ttaagcaaaa cgaaaggagg tacagctgat gaagtcgagg ctaaacaaaa agcctaaaca
     3421 caaagaacta gaagtcgaaa tcaaaattct ttggtttaag cttagaataa aacatctgat
     3481 tactaggtaa tcggataggg ggtgaaactc ccccacccct aataggggta agtttagttt
     3541 agcacattgg ctgtatctcc gcaagaatga aaggagaagt tatgagtgag caaaatgaaa
     3601 agtttatcgg agtgctaaaa gatatgcaca tgcaaatttt gcgagatttc gtaaacgcag
     3661 aagacccctt aacaaggtgc gaacttgcta aagggttcct cgaaattggc gactatttat
     3721 caaaacaatg actcgccacg ctcaagagct tcggctataa ctttaccatt attgatttgt
     3781 tcttgctttt cggcttcaat gaaaggtaaa ttatgttgct taatagcttc taaagagtct
     3841 tcgtaaagtt tgattttatc ttcgatagac aaagtagggc tagaagaagc aacaatagcg
     3901 agagctaaat cttttgaatt agaaattttc attagcttat cctcctttct gctaggataa
     3961 gttgattata acatttttag gaggtacaaa atgaattgga aaaaactaat gtttggcgat
     4021 ctagaacaca cgtttactag tcgtaatggc aaagaaaaaa caagtattga atttgaaggt
     4081 ggcttattgc cagctctgtt agtgctaggt ggtatcactt ggctgatcgc ttggtttatt
     4141 acaaaataaa aactcccaaa aggagtggaa aggagggaag gcatggaaaa aattaaatac
     4201 ggtgtactag gttcttccaa gatatttcac acaagaaaat ctgctctcac tcatgcaaat
     4261 cttttaggat atcctcgtag tgcggttttt ttgataattc cacagggaat gacgcagaaa
     4321 atgattgata aggtgaatta acatatcctg ctttattagc tttaaatagt tttttctttt
     4381 tagctacttt aaaagcaaca aagagtctat ctgtgatttc agatgtttgt atgattaaat
     4441 ccatccttgt catactgtgg gcttttaata ctccacaatc tgcctctgga acctcgataa
     4501 ggattgtatt gccatcaggc tgtactgcgg ctatagcctc tctacctgtt aaatcgttaa
     4561 ttatattatt ttgcttttgg taataatgct gatattttct gtttttatca aaaacaatca
     4621 agtcgaagta gcttacatca acattagaag gattgatgat tttaatgtta gctcttaatg
     4681 taccatttgg attatatatg ctttcaccgt tgtctaaaat aacgctcaaa atccaatctg
     4741 aaacaggagc agcaattaac tcgacttgta agttatttct tcggtagttt gaataagata
     4801 gaaacagagc tattaaagct atccaatttt ttattagata ttcacttgta aacttaaaaa
     4861 cacacaataa aaaattaaaa aaattcattt caacctcaca tttttattta aattatacca
     4921 cagaaacaga aagggggtgg ggaagatgga agaaattttg tcaagcatag caaaaagcct
     4981 tgaatccatt gcgactgaat tcaaagctca aaattcttac agagaagaaa tgaaacaaaa
     5041 tatggaacaa atggaaaaaa taatattaga tatccaatca gatccatttg gactcaaaca
     5101 cttaaaagat aaagcgttgt ccgaaaaagc ttctaagcaa aagggatagc atcctttatt
     5161 ttcttagcaa actcaagcca atattctatc ctgccagata aagtcttggc ttggtcttgt
     5221 ttttcaagtt cagcgatagc ttcggttgtt aacgaaatta gataaagcgt atcgcttgca
     5281 gccgtcgctg aaatatagcc atgttttcta agttcaaaac aagtatctag tacatcttct
     5341 tcagaccatt cattcataat gttttctttg atgaattgaa tatcttcgaa attgcgagac
     5401 ttttctttag aaattttatc tttacgtctc tcgttgtatt tgacaaacat tgaacttaaa
     5461 agaaacttgg catcatttgt taatttatcc atatattcac ctcctttctg ctcacattat
     5521 agcagattag aggtactaaa aacagataga aagggggtgg gagaatgacg aaaatgacgt
     5581 tgaaagcttt aagagcaaca aaaaactgga gtcaagaaga ggctgcaaga gctttgaaag
     5641 tttccaaaga tacatggggc aactgggaaa gaggtaatac agagccaagt gttacaaagg
     5701 cttatcaaat tgcctatata ttcgatatat cattagatga tattattttt ttaccaaaca
     5761 ttgcggttta aacgcaaact cttagaaata gaacaaaact aacacaacac tagaaaggag
     5821 aaaaatgcag gaaataacaa cacaaaatga ttttgactat tcacttgttg attttgaaac
     5881 tcaagaattt ttacaagagc gttcaaatat tatctatgga attcaaagca aaagcgctta
     5941 tgaaataggt aagcaacttg ttaaagctca agaagttttg gcaaaaaata gatatggttg
     6001 ttttgaagag tggtattcaa gccttggatt tagaaaaaca aaagcttatg agtacattaa
     6061 tcattataat ttcattcgtt cgcaaagcga acaattgaat atcgaaacat ttgaagaact
     6121 tccaaaaaag cttcaatcag aaatgtcaaa gccgtctgcc aatccagaac tcaatcaaaa
     6181 agtctttgat ggggatatta caactcataa gcaatacaaa gagttagaac gccaactcaa
     6241 actagcagaa gctgacaaag aacggttaaa acaacaaaat gagagactag ccgaacaagc
     6301 cttgagtgct aaagtcgttg aaaaagaagt cgtaatcgaa aaagttccag atgattatga
     6361 atctacaaag caacttaacc ggactttact agataaaaat aaagagcttt ccacaacact
     6421 tgaagaagct gaatgggagc tagatagtaa aaagcttgag ttatcgacaa taaagcttga
     6481 gtcacaacga gctattgaag taacaaacca gattcgtcac ctcgagggca aaaaagaaaa
     6541 acttgagaac cttgttactt cgataagtga gctgtcctca atcattagtg acgtgcaaaa
     6601 cttcttcgat acgaaaatgg cgccacttcg ttttaagcca attatcaaca atgtcaatgc
     6661 acattattca gttacggaag taaccaagat ggttaacaca gtccaaactt ggtgcgatga
     6721 aatgtacaaa attattccat ctggaaatag aaaaacagta gaagaggtaa taatcaatga
     6781 gtaaagaatt atcagaaatg aatgaaaaag acgctcttga agttgtcaaa ggaatgacga
     6841 ttgaagatat gatgattgaa gtcttatctt ctcaaaaaca agtaaaagct tctcaagaag
     6901 ccattaagaa agatgttgaa gaggttcgtt ctttagcaat tgaaattgat aaaaaagtcc
     6961 atattgatga tgtggaagct agtgaaatta aaagcattat cagtcgtcaa tcctatgaat
     7021 tcgccaaaga atattttgat aatagaaaag ttgttccaag cgcaaatcta ttcgctagca
     7081 agaaaggaca gtttattcgt gtacagcatt cacgattaaa acatcatttc aatgtgacga
     7141 agtacactca tatcaaacat acagaagcag aaaaagctat cagttattta aaatcactat
     7201 cttatggttt attcacacca tttgaaactc gtgaaacacc aaaacaaaaa gagattatcg
     7261 aactggaaaa taaaaataac ggtgatgatg tcgcttagaa ctagaaagga aacaacatga
     7321 acgaaaaaat tacagatgaa caacttaaca aatacttaga aatcagaact gaacttgggg
     7381 aagaaaactt taaatttatt aacgcttgct atgatgaagt tttgcaccta aaaaaagagg
     7441 aagcgacaaa aaatattaag tttgaaatta cagatttttc aaaatttcgt gattttgcta
     7501 aaaaatagcc ctcagaatta actgagagct tctagttaca tgatttctcc acgcatacgt
     7561 gccatacgtt tgtcgtgttc atgcacagtt tcagagagat ttttgccaga taaatgttta
     7621 ttgagtatct ctgccaaagc tactgataat ttatctgatt cagacacgtc gtgtctatcc
     7681 aacgtctctt taattctttg tgaactaatc ataccttatc ctccttccta taggatagct
     7741 acattatata acagaaaggt acaaaacaca atatgttgtg tatcttagac gattaaaaac
     7801 acaatatgtt gatttttaaa agtattatga agaaaaaatt gaacaaaatt ctcattgata
     7861 aaggaatgag taaaaaagag ttatcggaga aaacagggat tagctacaac accattatga
     7921 atatcggtaa aagagatatt tcgtttaaca aaatgaaaaa aatcgccgat gtcttagaca
     7981 ttagcctaaa cgaattcaga taaaacaaaa aaagccactg cgggaacagt gactaaccaa
     8041 aataatttaa ctaattatac cacgaaagag gtgataaatc catgcaatcg caacttactt
     8101 acgatctact caaaaagcag atagcagaag aactttttga tgagtttaag gagctcatac
     8161 aagaaaaaga tttagcgaat cggtgggtca accaaacagc actcgtcaat gaatacggct
     8221 actcatggca aactataaaa cgcatggaga gctatggcct taaatctttt aaaaacggta
     8281 aagacaaaat gtactgtctt gctgatgtta acgaaattaa acacttaatg aaacaataag
     8341 cgctggggag tgctagagga gaaacaacat gaaactattt aattttattt ttgcaaaacc
     8401 aaaaaaacag gaagaaaaac caaaatggac gattgaaaca catggctggg aagctaatgc
     8461 acgacgttac gacaatatga ttgaacataa taatagaggg cgtacatgtt aaaagaagta
     8521 ttactagaaa ttgaattctt acgtgatgaa aatagacgac taactaatga gttgctggag
     8581 gtattaaatg aataacgatt ggaaaaaaag atacattaac aatcaaatag actttttcaa
     8641 tgacatcaag acatgtgtca aagaaagatg gccaaatatg aaggggaatg atgtcgataa
     8701 aatttcgata gcgatttaca gaaattggcc cgtaagtgac atcaatagca cactagaagt
     8761 gatagaaacg gatttggaaa aattatgaaa tgttatgtca ataaacaaaa aaaactagca
     8821 atagacatga attataaaga taaattcgga aaatttagtt ctgactctat tcagatttta
     8881 gaaggtaaat taactgattc tattcaaatt gatgtcgaaa atgctatgaa agaaattatc
     8941 gataagtata gtcaattgtt tgatacgcct ataatcgatg atttattcac agaaaaagaa
     9001 aaacaattaa agcaatctta tgatgtcgaa actacattga cagaaatgtt cgaggtggaa
     9061 tatgaagata actaaagcaa cagagattac aaagactcat aattggcgca tattaatcta
     9121 tggcaaaccc ggaaacggga aaacttactt aattaattat ttaaaaggaa agacactgat
     9181 tttagacatg gaccattcat ctaaaacgat tgccggaaac gaaaacattg atattatcca
     9241 atttgacagg acgcacccta gcgactttat gactgaattc ttgacagaat taccagaact
     9301 tatcaaagaa tatgacaatt tagtcattga taatatcaca agttggcaat cagactggtt
     9361 tatcgaacaa ggtcgcaagt ctaaaaatgg aatcacaaac gaattgcaac aatacaacat
     9421 gtggaccaat tactatttac gagtattgac tacaatttac agccaaccta ttaatatttt
     9481 tgtgactgct tgggaatcaa cgcaagattt aacgctcgag agcggacaaa tgattacaca
     9541 atacgtccca gatatccgca aacaagtcct aagccaaacg ctaggtttaa ccgatgttgt
     9601 cggacgtatt caagtaaatc caaaaactgg tggacacggg atccttttgg aaggaagcga
     9661 tggactgtac gcaaaaaata ggcttgataa taggaccgtt tgtaaggcgg aagagttgtt
     9721 taatttcgaa gggagtgatg cgtaacggta taccatttac acgaatatca aacagaactt
     9781 ataaatgagg caagaagaca tattttaaag cataatgtga tgattgttag tccacctgga
     9841 agtggtaagt cagtagtcat ttctgatatt gctaagtcag cgactcaaaa aaacggatac
     9901 gttttattct tggtccatcg caaagaatta attgaccaaa tcactaatag ttttaaattc
     9961 catggaattg acatgaataa agtggattta atgacagttg gtaaagctaa aaaccgcttg
    10021 gataaattaa caaaaccaac cttaattatc acagatgagg gtcatcatgg gaaagctagt
    10081 acctaccaaa tgatctatga atacttttca gacgtgccga ggattggttt tactgctaca
    10141 ccttggcgtc tatcaggcga cggttttaca gacacttacg atgttatggt tcttggtaaa
    10201 acggtcgagt ggcttatcaa taataataaa cttgcaccat atgattatta tagtgttcta
    10261 tcaattgata ctgcgaaatt aaaagtacaa aacggagatt actccaataa atcgattgac
    10321 gaatcatttg gtaaaaagat ttttggtgat gtagttcaag agtatataaa aaaagcgaac
    10381 ggtcagaaag ctattttata cgctcactcg gtagaagcat cgcaggcatt tgctaaagaa
    10441 ttccaatcta tgggaattaa tgcaatacac gcagatgcaa aaacgcctaa agccaaacgg
    10501 gataaaagca tgaaagattt ccgtgacggt aaaatacaag ttatctgtaa cgttgatttg
    10561 atatcagaag gtttcgatgt cccagattgt acagtaacta ttctttgtag acctacaaaa
    10621 tcattagtat tatttttaca acagtctatg cggtcgatga gatatcaacc taataaaaaa
    10681 gctatcattt tagatcacgt aggaaactgg aatattcacg gcttacctga cacaccgcat
    10741 cattgggaga attatttccg aggagggtgg aagaagaagt cgaataaaac taacacggta
    10801 cacgcaaaag aatgtcctgt gtgttcagct ttgtggccac ttagtcaaca gctctgtgaa
    10861 ttatgcaatc atgattttgg attgaaagaa aaacaagaga aagaacgcat agaggcagaa
    10921 cttgaactca taaaacgtga gcggtttaga atcaaacaac ttgctaataa gaagtttggt
    10981 aaagatttaa aaacaaactg ggaaattgcc caagctagag ttaaagacgc tggcaaagga
    11041 aaaccattat ataaacttat ctatttctac ttaaaaactg attgggtaga aacaaacgtt
    11101 aatgaacttg ccgaagtaac aggcaagtca gaaaaagaaa tatatagcgc ctacaattgg
    11161 ctaaaaaaga aattaagagg ataaaaacat ggcaggattt acaacagatt tttcagaagt
    11221 taaagaacac gcagaattca aagaacaacc atacgaaatg attgtttatg atgcatatga
    11281 agcagtaaat gaccgtaacg gcaaaaaacg tgttgttatt gactatgttg ttcgaaacga
    11341 tatcaagcaa gaaatgcaaa actaccactt atgggatgag caatatccca actcacaaac
    11401 tgggaagtat catatcggca ttttaatggg taaagcaaaa gcacttggta tcaaagaggg
    11461 gcaacactac gatagttttg aagcgttttt aaacgacttc aaaggacgca ctgcaaaagt
    11521 aaccgttaaa cttgacgaat ataacggaaa taaatacccg aaagttcgtt acgcaaatca
    11581 aagcgatgtg cctgaaagcc accacatatg gaaagaaaaa acaactggat ttacacaagc
    11641 tgagattgaa gaagacgatc taccgttcta atttagactg ggggataaat gacatcaaat
    11701 gaatttattg aagctttatc aaaattgacc accgaaaccg attggggaga cccaatcttc
    11761 ggcgagtcag tgcttaaagc tgaattacga aaacacttat ttaaaattgt cccaattgat
    11821 cacaatgggt atatccacaa actgttttat tcagaaatgg ttaaagatga agatgtcatg
    11881 tattttgtgt cagatggacg aaaaacttat cgctttttat ttggagatac aattctaaag
    11941 actgataaac aaggcaatga atatctcaca tactccgttg aaaataattt tccgccattt
    12001 gcaaaactag ttatcgacta cattctaggt acttacacgt tttttgagaa taaactttat
    12061 gacattcgat ataagcaatt taaattgatt gatgatttta cacttcagac taagtatggt
    12121 ttcaaagact ctggtcacat tttagagata ctacaaggta tccacaaaac attaaacatc
    12181 caaccaatca attatattga accatatcag attgcttgca aggatttcat aatcgacctt
    12241 gaaaattctg aaatcattaa ccaaccgcct ttgcaaaacg tatcttattt taagtattac
    12301 gaagtagact acaaaacagc aataaacagt aagtctattg cagaagaata tcttgaatac
    12361 gttattgcag atagcaattc gttaaacaat gcaatactac aatcttattt tatcgcccaa
    12421 gtggcgtgtg gtgttagacc taaaaccaac ttcttcatct caaaatctgg agtaaggact
    12481 ggtaaagggt taaggcatat agctttatct ggtctattca ataagattga tgttgagcta
    12541 gatacattaa aaagtaatgg atttgaagcg ttacaggcgt gggcgatgtt ctcgggtgga
    12601 gaaatggctc tagcgacgga acagggagat attcaaggca atgcaatgga gcgtgtactt
    12661 aaaattattg caacagagaa aacacacgtt gcacgagcaa ttgggcaaaa tcagtcaatt
    12721 gttaatttaa cgagtgttct gtgtattgac acaaaccgta ctgttgcgct gtcggatgaa
    12781 atgaatggac gaaaggtttt aattcagttt aaagatagac caaaaactga aactgattat
    12841 gaaagagaga gcgtattcag gaaatattgg ctagcattta ctgatcgtga taaaaatcca
    12901 aaaatcgatg gttgtatcgg ttttctgtta aactcactcg agcgtttcca aaaaattggt
    12961 aaatggtatc aatggaaaga tgttgaagta tttaatgata tcgatttaga cgaatttcaa
    13021 gttgctttaa taaatgcatt acaagaagtc gattttgtac agcgaactga taacaaagaa
    13081 gttattgact tatcattaca agtttacgga aaaagcaatc atgcagtaag taaagccata
    13141 tctgaaattg gtgtacgtag caggtcgaag aaagttaacg ggaaaacagt cagagggtat
    13201 gaaatcgaaa ataaaacacg ttttgataaa tatatccttt aaaaaggtaa cgcatggtca
    13261 cgcagagtta cgcaaaattt tccatgttgc gtaacccact caatcccttt agtaccaacc
    13321 gatttcagcc tttattcaag aaaggttacg cagtaacaaa caataagtct aattaatgat
    13381 ttattaatta tttatttata tataggggta gggtagggtg attttgcgtt actgcgtaac
    13441 catccttctc taatgctcta tgtatcaacg ttttagaggt tacgtagtaa aaaaggaggt
    13501 gcgttacctt atgtaccaca caacagcact ttcattctta aaaaaaggat atcaggttat
    13561 accgcttaga aaagatactg gaacgccaat gattaagttt aaagatatcc aaataacgga
    13621 agaagtgatc aaaaatacaa actggtttaa ttgtgattat gctttattaa tgcgtggcat
    13681 ttggtgtatt gatattgata ctcatgacat ggacgagaag ttagctaaag agttatacat
    13741 aatgataaaa aagatgggga ttgatttatt atctgtatta tcgactgata agtatgacaa
    13801 tggactagat ggttattcgt caattattag gcatgagtat aaaaacgaat taatcagtaa
    13861 ttttaaaaat acattcgcag aattaactgc aagtggtggt atgcacatac tctttaaaaa
    13921 acgcgatgat attaattaca cgcaaaaaat aggagtaatg cctggtgttg acataaaagc
    13981 gaatgataat aattttgtca aaatattccc atctgacgga cgtgaagttt tacaagcagt
    14041 taaaacatta ccttattatg atggaaaatt tgaagaggaa gtatttaaac caaaacaaga
    14101 aggtataaca acctattttg gatgttctgt cacatataca tcaaacggaa gtcacgaagg
    14161 ccgagaggct tatgaacgtg tagcaactgg aacatcatat aacaggaatg atgatttgtt
    14221 taaaggagcg tgttgggcgt ttgaaaatgg tatcgatatt gatgatttaa catcaattat
    14281 tgggacggtt aaaggcagag acgtatttac gagagaggag tttgaattaa caattgaatc
    14341 agcgaaacga aaagtcagct acgtcactat cagaacatga tatccaaaac cttatccgaa
    14401 tggaattatc acaagcgggg tatatggtat ttcgagcaaa tgtcggaaag gtgaaaacag
    14461 cagatggtag attttttgat acaggtttac caaaaggttt ttgtgatttg tttggattca
    14521 aaccaaacgg acaaatattt tttatcgaag ttaaaaatga aacaggtcgg ataagaccag
    14581 aacagaaaaa ctttatggag gttatggcat cgaaaggggc gttagccgga gtggcgcgtt
    14641 cggtggaaga tgctttgaaa atagtaaatg gatatcatta ataaatttaa aaagttagat
    14701 cattacaagg aaattaagcg attgatgtac gcagcaatga ctttctgagg aggtatagta
    14761 tgacagataa aattaacgca gaaactatgc aagtagcata taacgaaaac tatcaaacat
    14821 ttttagccaa aaacgcagat tacggaaatt ctttcgagaa gtctctaaac gactttggat
    14881 tcgtcgctgg tgtcgtacgt ataagcgata aatacaacag actatataat cttataagca
    14941 gtgataaaaa cgtctcagaa agcctgtcag acacgttaaa cgacatggct aattattgcg
    15001 tgatgttagc ggtttggttg gaggttaagg atgataccaa aatttagagt gtgggataaa
    15061 gtaacgcaag ctatgggcgt ggttgaagca atagattacg tcgataacaa aatatacacc
    15121 ttttatcgga aagttgtccg tagatatatc cctttcgagg aagctgtgtt aatgcaatca
    15181 acaggcctaa aagataaaaa cggcattgag gtgtttgatg gggatatcgt taagtgttgc
    15241 agacttttta acgactcttt gtctgagtac gtaggacaag taaaatttgt agattttggt
    15301 tggaacatag ttgataaagc agatacacac gatccatttt acaactacaa agacgggtgt
    15361 ccagacgaaa ttcgggaaat cgaaaatatc ggaaatatac acgaaaatcc agaattacta
    15421 gaaaggttag aaggatgatt gaagaacaag aaatacttga ctgcattacg caggacacaa
    15481 aagcaactta aacgtgctag cgagcaaaat cagagacaga caaagcggat tgctgatttt
    15541 gactggaaat gggggatagg gatgtcagac ataaaaattt tagatgcttg ttgcggaagc
    15601 cgcctatttt ggtttgataa aaatgaagaa cacaccactt ttatggatat tagacaagaa
    15661 acatttgata ttcacggaaa acatgtaaat gttaaacctg atgttttggg agatttcaga
    15721 aatatgccat ttgaaaataa tatatttaac ttagttgtct tcgacccacc acacttaaaa
    15781 tatgttggcc aaaagtcaat tatgaaagcg caatacgggc aacttgataa agacaattgg
    15841 aaagaagata tttcaaaagg atttgaagaa tgcatgagag ttctaaaagt aggtggaact
    15901 ttagttttta aatggtctga ttgtcagata aatgtaaaag aagttttatc agcaattccg
    15961 tttacaccac tttttgggca gcgacgagga acaactcact ggatgacatt tgtaaagttt
    16021 gcagaattga ctgggaatgg aggatgagga tgtcattaat tgatgaagta aaagaatgcg
    16081 gttcagaatc acacaaaaaa tggtttgata gattttttga tgatttaaaa atcgaggaga
    16141 agcttaaaca atctgcgatg aaaggtttct ctggctataa aatcgctatc cccaaggacg
    16201 atgaatactt agctagacga cttgacagtc aaaaaacaat agatttacta aaacaacggc
    16261 taggggacgg ttttgatgtt gaaataacaa atcttgaatc gtcgtataaa atatttggta
    16321 aaccagttgt gttaagtaga gatttgtgta tttattggac gaatggagga taagatggaa
    16381 aattatttat gttactggga aaaaagagga caaaactggt atttcttggc taactgcgaa
    16441 gactcaccaa ctggaagaat atttaatctt actggtgtta aattcaatta ttgtcctttc
    16501 tgtggacgta gattaaaaga ggtgacaaat gaaagttaaa gatttacata aagtgctagc
    16561 taaagttgac ccagaacttg aagtgtggat aaacgaaaaa gacgaaggag ttttaactaa
    16621 agctaaaaac gtaatgactt ggaacaagca tcctgataag ttttttatca atgcagaaac
    16681 atatccagaa tagaatgagg tgacgaaatg aacattgaag aagcgaaaaa aatagtggac
    16741 aaattgtcag tagatagtga tgagctttgg aagattccaa tgattccagc ccataaggta
    16801 aaagccttgc ttgacacact tgaccaacca aaaccagaag tgccacaaat gatatttgat
    16861 gtgattaaaa gctttgatga tgatgtagat tatttacatc aacacatgag tcgacaatct
    16921 gatgaagtta gagagtggct aactcacaat gaatgtgagt tttatgaagc ttggctagct
    16981 tatccaaata tcacaattga aaaagagaag ctgtatacgg ttgagatacc aaatccgaat
    17041 agtgatttaa aaataatttt agtaaaggta aacaagaaac taaaattaat tgaagcatat
    17101 gaagatcaat tagaggaata caaaaatatt agaaacgtca ccgaatccga aatccgcaaa
    17161 gacttt
//
LOCUS       DATRME010000041        16962 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRME010000041 DATRME010000000
VERSION     DATRME010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16962
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(41..607)
                     /locus_tag="VBC23_001316"
     CDS             complement(41..607)
                     /locus_tag="VBC23_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843262.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(600..1535)
                     /locus_tag="VBC23_001317"
     CDS             complement(600..1535)
                     /locus_tag="VBC23_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6843263.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1628..2269)
                     /locus_tag="VBC23_001318"
     CDS             complement(1628..2269)
                     /locus_tag="VBC23_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6843264.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2250..2834)
                     /locus_tag="VBC23_001319"
     CDS             complement(2250..2834)
                     /locus_tag="VBC23_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6843265.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2904..3049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3118..3717)
                     /locus_tag="VBC23_001320"
     CDS             complement(3118..3717)
                     /locus_tag="VBC23_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO6843266.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKISNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3710..4381)
                     /locus_tag="VBC23_001321"
     CDS             complement(3710..4381)
                     /locus_tag="VBC23_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843267.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4371..5246)
                     /locus_tag="VBC23_001322"
     CDS             complement(4371..5246)
                     /locus_tag="VBC23_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6843268.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5284..6792)
                     /locus_tag="VBC23_001323"
     CDS             complement(5284..6792)
                     /locus_tag="VBC23_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6843269.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6833..11137)
                     /locus_tag="VBC23_001324"
     CDS             complement(6833..11137)
                     /locus_tag="VBC23_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO6843270.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDMSVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11157..11726)
                     /gene="lepB"
                     /locus_tag="VBC23_001325"
     CDS             complement(11157..11726)
                     /gene="lepB"
                     /locus_tag="VBC23_001325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6843271.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11710..11982)
                     /locus_tag="VBC23_001326"
     CDS             complement(11710..11982)
                     /locus_tag="VBC23_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843272.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
     gene            complement(12264..13412)
                     /locus_tag="VBC23_001327"
     CDS             complement(12264..13412)
                     /locus_tag="VBC23_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6843273.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
     gene            complement(13402..14250)
                     /locus_tag="VBC23_001328"
     CDS             complement(13402..14250)
                     /locus_tag="VBC23_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6843274.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            complement(14247..15671)
                     /locus_tag="VBC23_001329"
     CDS             complement(14247..15671)
                     /locus_tag="VBC23_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6843275.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            complement(15671..16927)
                     /locus_tag="VBC23_001330"
     CDS             complement(15671..16927)
                     /locus_tag="VBC23_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6843276.1"
                     /translation="MPFFNEPDGQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTS
                     YRDGTRFEKYYLNKANFVDGNHLPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGV
                     MITVARLFSSFVSITLVALIIRYVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFG
                     AFLSLLINLLVDKSLTKNRSIALIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLM
                     GYNQKLSHLFQGLKSKKLFWLCLTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAG
                     INILSNDITSWLASPYPSQNFIPTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASF
                     FVGILGVFFIMLEYRGAPTSYIEGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPY
                     ALTGLVIVSNALLLFDTVISLIMRRN"
BASE COUNT         5421 a         3228 c         2664 g         5649 t
ORIGIN      
        1 tttatagcaa ttatcatttt tgctaattgc ctaaagatag ttacacctca ttttcatatg
       61 attgtaactt ttctaccata attaaaaagg ggggcgtatt aacttgattg agtggttgat
      121 aaagcattgt agcgaaatta ttctgtggta attgttccac gaatgctatc accgcatctc
      181 tctcactttt tccaccatca tgaccataat aaaccataag tgatagacgt ccacctactt
      241 ctaaaatgtc taaaacgttt ttgattgcct tgatagttgt gtgtggtttt gtaataactg
      301 ttttatccgc tgagggtaag tatcccagat taaatatagc cgctcttaaa ggtgtatgaa
      361 catactgttc caaattttca tgcccatcca agataagttc agcgttagag atcccttgct
      421 ccgtgagctt tgctttcgtt tttgctattg cttcttcttg aatatcaaat gcataaactt
      481 ttttagaact tttcgctaaa aaaacagtat cattcccatt tcccatagtt gcatcaagcg
      541 ttattgcttc tttatcaata acttctgcta aaaaatcatg agaaagatga attggtcttt
      601 taatcatgtt ttacctcaac tttacatcct tgatatgact gtcttttctc catttctttg
      661 tcaatggcat taagcacttc ccatttatta aggctccaca tcggtccaat taacatatga
      721 cgtggcgcat caccagtaat tcggtgaata acaatatgtt ttggaataat ctccagttgg
      781 tcacaaataa tcgaaatata gtcttcttga cttaacaaac gtaaacgacc ttcatggtaa
      841 tctctctgca ttcttgtatt aatcataaga tgcagaaggt gtaattttat accctgaata
      901 tcattatccg taacacatcg acgaacattt tccaccatca tatcatgtgt ctcccctggg
      961 agaccattta ttaggtgaga aacgatttct actttaggag ctaattctcg tactctctta
     1021 acagtttttt tgtaaaggtc atatgaatgt gctctattta ttaatgcaga agttgcttca
     1081 taagttgtct gaaggcctaa ttctaaagtc acatgcattc tttcagaaag ttcagcgaga
     1141 tagtatatag tttcatcagg taagcaatcc ggccttgttc cgatgttgat cccgataact
     1201 cctggctcat taatagcctg ttcgtaacgc tctttaatta tctctaactt agcatgggta
     1261 ttggtaaaat tttgaaaata gactaagtat ttattaacct caggccactt tctatgcatg
     1321 aaatcaattt ctttatagaa ttgttcacga ataggagctt ctggagcaac tatagcatcc
     1381 cctgaaccag aaactgtgca aaaagtgcac cctcctctag caactgttcc atctctgtta
     1441 ggacagtcaa aaccagcatc tataggtaat ttaaatattt tttctccaaa gagttctcga
     1501 taataatcat taatcgcacg ataacgtttt ttcataggat aattgtatca caattttaac
     1561 taaaataacc tcactactac aataaaacta aaaaagattg gaacgtcagt tagtcccaat
     1621 cttttattta cttcactttc tttaaccaat ccttggataa aaagatatac gcagttagat
     1681 tcaaaatacc ataagcaagt ataaaaccag ctaaaacatc tgtcggaaaa tgaaccccta
     1741 ggtaaatacg agataaccca attaaaaaaa tgagcaaacc caatgtacct tggcacaaca
     1801 gtttccatat actcttaggc atatagtact gcaataaaat aatactactc ccaaatatca
     1861 taaatgttcc catcgagtgc ccactgggaa acgaatagcc acctgcaaat actaaatggg
     1921 ttaaagttgg tctcactctt tgaaaaataa gttttaaaga aagtatacat ataccagaga
     1981 taatagcatt tactgcgata aatctagctt gaggatacca cttcttaagg taacagaaag
     2041 tgacgctcat aatcgcaata gctatctggc ttacagtatt accaaccaca gtgattaact
     2101 taaaaaatct tgtagaaaga tttggcaact gtcctctaac actttcttga atagtttggt
     2161 caaatgcgat tacagtgtcg ggccaatatt tgatgaccaa tcctaaactg aaaaataaga
     2221 taatagcaat aaatgcttga ataagtttac tattttgacg agataacatt agtcttttta
     2281 tatctttcta atattggcaa acaagccacg taagttagat agaaaacaat cgaaattaaa
     2341 attccctcaa cgatattaaa tggaataacc attgttaaaa ggtaattgcc tacaccaata
     2401 aatgttctga tatcaaagtt agcaaatata gcatacaaag gaatcgcaaa gacatagttg
     2461 agagctacca tagatacagt caagctaact gtaccaaata aactagcttt aataaaatct
     2521 tttgcactct ctctattttt ccagaaaata gcgaaacttg ctaaaaatag agctagagca
     2581 accatattca tcggtaaacc gataaaggtt tctggaccac gattagcaag tataactttt
     2641 aaaagtgatc ttaataagag tacaccataa cttgatttca aatcaaataa aataaaagca
     2701 actaacatcg gaaggattga aaaatcaacc tttaaaaatt ctgctcctgg tattaatgga
     2761 aatgaaacca tcatcaatac aaaagataag gcagaaagaa tggcgattgt caccatttta
     2821 cgtgtatttg tcataaaaaa attcctccaa tttaaataaa ttgaaagaag ctccaaaggt
     2881 aagcgtaggt acgcgaaaaa aacctttgtc ttctcccatc cagactttac tgtcggttgt
     2941 ggaatctcac cacatcagct ttcgctcgcg gactgatgct tcacaactga caaataagtt
     3001 ggaagcgatt accgccggtc gggaattaca ccctgccctg aagacaccta tagcataaca
     3061 aaaaaaactt gcaattgcaa gttttttaat cactaattag tagtagattg tataatatta
     3121 atttttaaca tcaattaatt gacagcgcac taatactcta gctactcctg cctttgtaca
     3181 agtaaacaag cttaagtccc aatcattgtc tgatgtggca gttttataaa ctttttcaat
     3241 cgctgttggt tcaataattt ctctattact gattttgtag tgatagattt gccctgttgt
     3301 agttgtaaaa taaacatccg ttcccatatc tacatttttt aaagcatcaa aatgataagg
     3361 aaaattatgc gcacaaatca ccatgttatt agttaaataa gaaccataat accttgtagg
     3421 cgtcttagac agttgttcaa aactataatt agcagctacc ggtaaatgca gttttaagtt
     3481 cggaatatcc agagttccca agtaatctgt tttatccaac ttttttacag gtaattctcc
     3541 attttctgaa ccctttactt gatgcgtaat agatttatca agcaccttga caatatgctg
     3601 agaagttaaa tcagcttgat gcgcctgatt aatattatac caaccccaat agattccaga
     3661 acttaccaga ataattccga gtatagctaa aaaatttgct gaatatcttc taatcacgtc
     3721 ttcttctcca ttttaaagct attattaaac acagaagtcc tgacataatt agtataggta
     3781 ttggccacca tacttgtcca gtaaacggaa gctttccctt tgtctgatgt gttactgtag
     3841 gagtaattgt ctcttctttt ttaggtttag catttaaagg gctcattttc tcaaatgctg
     3901 taatatcgta cttcccatcc ttaggtattg atagtataaa gggagacatt agttcataac
     3961 cttgagccgt tttagtctga ataaatagat aaatcccttg aggaagattg ttcgcaacaa
     4021 taccttcagc cggtaaatta tcaaacgttt gtaaaggttg agttttatga acagcttttg
     4081 ttagtagatt gacgtatttg gcttggttac tatcaaggtt tacttgtgtt agatcatcgt
     4141 cttttattcc aataccttga aatggggtag ttagagtaaa aacttgatta ccatcaacat
     4201 ctttagcttg tgctacttgg taaacaagta aattaccgcc agcgatacct tgattattat
     4261 acttattttg tatagtaata gaacccgttt tcatctgatc attggtatca gcagacacaa
     4321 gttgagtact tagactaaat aataagagaa gagttatctt taggatcttt ttataaatca
     4381 ttgttctctt cctttctcat tgcttgtttt aaaattttct tacgttgacg tgctctccta
     4441 gttacttcta aagagattaa aagtaaaatc aaagtaagga aaatagcgat aaatggtgcg
     4501 atataaatag gctctatttg tattgcctct gctactacca aagcgttacc attatcgttt
     4561 ggtacacgat gtcctctcac tagtaaccga tgggtattaa cgccatatgg tgtacacgtc
     4621 accaaagttt ggtagtcttt acctttaaca atttgtaaat ccctcaaatc atccggttta
     4681 actgttctga tttgatccac ttgataagta tatgtttcat ttaagatact gactgtccag
     4741 tggtctccaa cttttaactt atccaaatca gaaaaaagcc ttgaagaggg taaacctcta
     4801 tgtcctgata aaatagaatg agttgagtct cctccaattg gaagactact tccttctaaa
     4861 tgaccaatag aagtttgaag cactttttca cttgtaccat gataaagtgg taattttatg
     4921 tttatctttg gaattgaaat ataacccata ttacccgttt tatcgatagc cagttgtgaa
     4981 ttataatcca aacgctcttg gctagtcatg tgccacttca ttcctgaagt tttaaattgc
     5041 ttattatatt ctttggctcg gttaataatt tttttataat cgttttcatc catatgcgtt
     5101 acgcggtctt ggtaatccat aatcgctcga gattggtgaa atgaattcca ataattagca
     5161 agtgaaggat aagccattaa gcctaccccc actgcaatta tagtgacaag caaaatggat
     5221 actaaatgtt gtcttatttt tttcatatat tttaaatctg taccactttg ctagcccatc
     5281 ttattaagaa cgtaaacgac gacgagcaac aagcacgata cctgctccta ttactaaaat
     5341 tgcacctata atgtagaaaa ttgttgtacc aataccacct gttgaaggca actcagtacc
     5401 tttgttattt tcaacagttg ggttaactaa aaggttatct gaattagtcg tatcagtggc
     5461 tccatctcct aaaataacct tctgagagtt atctaacaaa ttgtaaccta agggagcctt
     5521 tttctcaact agatagtatg taccttcttt caagcctgta atggtaatta taccatctgc
     5581 tcctgttgta tattctgttg cattagcttc tgtgccccat tcaacgttat ttgtatcgtt
     5641 aaagtttaga aattgacccg tagcattctt taaaacaaat atagcacctt gtaatgaagc
     5701 ttttgtggaa ccatcaattt tttttatagt aatttgacca tccctcactg ttactttttg
     5761 acctgggtca tcattgctag tattggggtt gatggtcgca atgtttgtat tttctggtaa
     5821 atcagctgaa cctggtttag ctccactctt taatactcct gtataagtga ctgtgattgt
     5881 atttattccc ttataaaaaa agtcatcatt agctccattt tgagtatttc cggttggagt
     5941 attggtagct gcccacggaa tagtaatcgt gaaattatta ttttcctcta acaggttata
     6001 cttcccagtt gctttttccg aaccttgagt tagagttgta atattccctg atccatcagt
     6061 aatagttact tcataagacc cttcgttcaa atcaactaca gaagcagatg gcatagtatc
     6121 ctttataaca tattgataca ctttttctgt accatgataa ttgactgcat tcttataagt
     6181 aatagtatat ttgactgtat caccaaccga gtacgttttt tgatctacag tttttccacc
     6241 accatctccc catgtcgcat cagtattctt ttcatgaata gtagcatttg gagttacaga
     6301 tgtaaccata attacagctc cattattaac agtgctagaa acataataat atccatattg
     6361 ggaaacatta ataacctcag taccatcatt atttgactca gtaacagtgg aaactggtgt
     6421 agtattagct gatatagatt tagcccatgt cgcaatctca tttgctgacg cagtatcttt
     6481 tttagttaca tatgttctcc ctccattagt agttgtcgta aaaagagaat taaaatcagt
     6541 tgaagcttta tactcagctt ctttaccttg aggaattaaa taagaagctc catctttatt
     6601 cgaatcagat acatttgcat tatctatttc tgcatcaaaa actttatatg ctttataggt
     6661 tgcgcctttt tgagtatctt gaactgtaat tgtccctgtc tcagcggcaa aagctatcgg
     6721 cgtaactggt gatacagcca taccaaatgc taaactcgcc actaacagcg attgaatcat
     6781 tttctttttc attgaaatct ttctcctaaa atcatattga tgaatgatta attcatattt
     6841 tttttcgata gtataatatt aatcctgatg gtagagctaa agctaaacca actaggatat
     6901 aaatgtgtgt tccaatacct ccagtactag gcaattctgt tcctttactg ttagtaattt
     6961 taaaagtata tactgtactt ccatccacta aattctcttt tattggtgtc gcattattac
     7021 cattttgttc aaaggtaact cccgtagaaa tcactaatac tgacatatca tttttaggta
     7081 gtaggtaccc tggaggggcc tttgtctctg ttaggtagta ttttcctact ggcaaactga
     7141 ggtagttatt agcatccact aataacaagc ctttatcgtt tgtcaccagc cctgaataca
     7201 taggatgtga agctttattc ccattagcat ctgattcata aatatcaaaa actgcacctg
     7261 ctaaaaaatt attatcattt tcgacattaa ctttctgtag tcgtactttt tgcttgatac
     7321 gtgtattggt aaagctaata tctacgtctc ctgaaactgt cagggattgt aagccggtag
     7381 catcataagt tttatcagct tcaccagttg ctagattttt ttctgtaatt gactcagata
     7441 ctttaaattc atcgtaggct tgttcatcta ttgatataga agttccataa ggtactttaa
     7501 attccttagt ctgaccatct ctcagcggaa aattctcttg ttgcaacgtt tcacttggat
     7561 taaacaagaa gtctttcgtc ttatcttcat ctagtccaac gacagtttta cttactctga
     7621 cggtgtattc tttaggttgc caaacagcat ataaggtatt tgttgcatca gggttgttat
     7681 caatacctat tgattgacct gctgtaaatt ccacacgtcc tgtatcagct aaatccttat
     7741 catgatgcca accaataagg ttgtaacctg tccttgtaaa gtattggttt tcaggaattg
     7801 tagttgtgct attcaactcc atacgcggtg tctccacttg tgttaccaca ttaccatttt
     7861 ctactctagt accaccgtta ccattgtatt tgatggaggt atcttctaat ttgatatctc
     7921 ctactggaat aatgacaggt tttatggtga tatttttatt agcatctgct aaatgggcgt
     7981 caatatcaat ggaatcatat gggttataaa ttttaccatt gtaccaccag ccacggaaac
     8041 gatagccatc tggcattgtc ggacgtctca gtaaggctga atgggagcca tcgtcatagg
     8101 aatcagggtc agtaggctca ttacttgttt gtaattgctg accagaagca tccaatgctg
     8161 gctttccatc tgtaccaaca gcatcattgc tgtatataat atgataatct ccagcctttc
     8221 gccaaatagc tcttaaattg atatcttgag ttacagcacc tgaaaagtta taaggtctaa
     8281 tactaccatc tggattaaca taataccaac cgactaattt gtaagcgtct tttacgtact
     8341 tgtacttagt tgttgtatca acatttgaga gactagtatc tgtcgtataa taggcatctt
     8401 tagttgagtc gggatcttta tctcgtgaat catacttata ataatatgta cctgaaggat
     8461 cttggatata atcccttgta atatctgtat aatccggaat acgatcacca taatgcaaat
     8521 ctaaataggt atcatctgtt tttgataatc ggcctccgtt tggatcaata ttgacacgat
     8581 atgttacctt ttgccaacct gcatagactt taacatcatg aggaggcata gtcgtgttaa
     8641 agtcaaatac ttgtgtttgt gcttggtctt tataccattt accatcccaa acataccctg
     8701 gtcgactagg tttaggttga acagttgtcg tatcgggggc ataagaggac aaattttgct
     8761 catatagaac atcctttact ggaaaattag gaagctctgt attatcaagc ggatctaaat
     8821 atttaatctt gtatgaatta cgttcatacc ataccactaa gttcaaataa tctttgtggt
     8881 aggcaccata tctatcatag tattcatctg aaataggaac ttttactcct gcactcgttt
     8941 gacttgcgta ctgatcaaaa aggtaattat caggatataa acgttgataa tatttaacat
     9001 taaaccctag atatttttct gtaaaggtaa attcatctgc cccagcacct ccaccggtat
     9061 ctgctaaaga ataagtgcca tccaaacctt gtttgaagaa ccgataagtt tgaactatat
     9121 tcccttttgg aaagagtttg ataacatctg aatcaacggt atcattaggt aaaatgaact
     9181 cacccaaata actcatccca taggttccaa cttggttatt tccaacattg gctaaataac
     9241 cccatgcacc cgtcttccat tgataaccat tagcggccaa ggttgtaccg taaagtcctg
     9301 tgtaagtatc agcatcagag ctccaattat acgaatagta atattctggc aaggaatatc
     9361 cgccatattt tgcaaatttt attgtgatga gttttctatc ataataaaca ttaacaacac
     9421 ttgaaccatc gtcttttatc atgacagaag tttctgtcct cgtattatta actttaaagc
     9481 cagtcggtag tttatcatta atatcttgtt gtgttagcgt ctgattagat aaagataggc
     9541 ctgatcgtgt tacttgccct gcgtactcat atgtcttttg cgcatcagta gcatttttat
     9601 tatccgttgc tgattgttgc cagtagttta tcgtgtaggt tgtttgtgcg ggcgaccaat
     9661 gtgcatatag cgtcgtatcc ttggtcaaga cttgattaaa atcaaaggct gccccaccac
     9721 tagcagctgt gtaccaccct gcaaaagtat aacctggcct cgttggatca ttaggcttaa
     9781 ttgtcgaagc aggttggcct gttaacacac gacgaggtgc aatataggta actcctgttg
     9841 ataagtcgcc tgtgttgaat tcaacaccgt acgattcttt aaagacagga taaagactta
     9901 tgtctgaaga tacaggtaat gcttgaattt ctgattcaga aagtggagct ccattttgag
     9961 ttttagacca acctacaaat aacaatgttg aagtgggtgg tgaaacttta aatgaactta
    10021 aatccgcggt tccgtttcct gaactatcca atggtacttg ataacgctcc aaaatacttt
    10081 taccagcaga atcatcatat aaagttactg tcgccacttt actcataaat ggacgaacac
    10141 aaatttcttt tgtctcagtt acagttattg gctcaccaaa tttaacaggg tcaccatact
    10201 ttccagtagt aggatcatag gtataccaac cattaaaatg ctctcctgct ttaatcgtcg
    10261 gaatcccaac ttctcctaga gattctccat cttttataat ttgatgatga acttgcatac
    10321 ctgaagctgt caggaaatca taatcagttc cgtcattatt ttgaaaatgg taagttaacc
    10381 taggaacttc tgtattatcc tcttgaacaa ttgcataaat ggagaatgaa tctgttttaa
    10441 aagcaacatc actgctagtg ttcttagttt ctgcagtatc tttagatttt aatacttctg
    10501 tttgaccatc atctttaaag tgaacaactt taaggttttc atctgaagct tctaatggct
    10561 tatcatagtt aacctctact tttactggag cttggggttc tgcttcttta ccatttgact
    10621 caatagtaat gtcatagagt ttgaaatttt tgatttcact atcttgttta gcaacctctg
    10681 tcaatgcttt tttcttataa tctttaaaag tagctgaatt gtcttttaat tccgtcacct
    10741 ttaaatcagc atttttagga atcttagctt ctttggtcaa agtcactgtt acagtatagt
    10801 ctgcacctct aaacatcaat ggttcttcac gataagcagc ttcctcagaa gatgatgttt
    10861 ctgttacact agaagcagga gtctggggct tgctctgctc aacacttgat tgagaactag
    10921 atgttgatga agttacctgg ctagaatttt tattttctaa agtaatcccc acatcatctg
    10981 tcttagtttc ttcaactgtt attgctggta gaattaaaaa ataagtcgtt aaaaaagttg
    11041 ttaggatcat caatgaccac atgataattt tccactcttt agggtgtttt ttctttttta
    11101 atgattcgat tataaaagtt tgacacttct ttagcatttt tgcatcctcc ctaaccttaa
    11161 ttgatactac taatcttacc tagaggccat attctgaaag aaattttacc tacaatttgt
    11221 tcttctgaaa catctcctac agaagtactt cgagaatcaa ttgaagtttt tcggttgtct
    11281 cctaatacaa aaattttttt atcaggtact tgatatgggt attttatatt actattaccg
    11341 agtgctttat gaataacata tggttctttc aacttatgtt gattcacgta aacatcccct
    11401 tgagaatcaa tattaaccca gtctcctgac tctgcaataa cccgcttgac taggacctta
    11461 ttattgtagt aaaacgcgac aacgtctcca gttttaaaat ttgaaccttt tactgtaaag
    11521 actacatcac ctgcacttaa agtcttattc attgaatgtc cgtagattct taatacaggc
    11581 aaccataaaa ccgcaattaa aatggctatt gaggcaaccg ccatcaagat gtatatggta
    11641 tttttaatga cactccaaaa gcgtttctga taagttacgc gatccagttc ttgagataat
    11701 ttatctgaac taatctgtct tttcattgtc tacgcctctc ttatctacta aattctttac
    11761 gttttctaaa taggtatctg cagctaactg agcagtctca aaaataccac taagcattaa
    11821 ggaagcttcg gcaatagaac cagctttgct aattttgatt tttttatcat ctagcgcttc
    11881 tttaagttgc tgaatctctt tctcttgttt ttcaataaga agttgctgtt ctaacataat
    11941 ttctagcaag tcttttcttt ttaatttttt taaatcttcc atcgcgatta cttccttaac
    12001 tgaaccttaa attatcgttt agatattata tcaaagttct aacctttaaa ctcatttttt
    12061 gtcctgtgtt ttttctcaaa aaagtctatg ctaaattaac atttttgata attttttgaa
    12121 aaatctcatc gaagtcattt tctttttgaa agctcgaatt ctaggcatta aaaagccata
    12181 tatcaaattg atatatggct ttttttatta tttaaaacaa aagaatcaat aggacaatag
    12241 cgtcaattta gtgacataat ctattacaga ttaagttctt tttgaataat ataatccaac
    12301 ttttcaactg ttttttccca tgtgaaatgt tctttaattc ttttagcaat attctgttgt
    12361 agtttctctc ttaatgcctt atcttttact aataaatcaa gagattcatg gagtgactga
    12421 gtattttctt ccatgatgat tcctaactca gggctatcaa taacttcaac tgttccaccg
    12481 cgatctgttg caataatagc actcgaaagt agaccagctt ctaaaataga ggttggtaat
    12541 ccctctggat acattgatgg gtaaacaaag atatctgtct gtgccattaa agacatagtc
    12601 tgttcaaagt ttaatttccc caaaaagtta atctgtttgg actgatattt ctctttcaaa
    12661 tgtgctaatt caggtccgtc tcctgcaatc tgtaaataaa cattttcaga gtactgtgac
    12721 atcgaaaatg cttctaagag caattcaatg cctttttctt taataattct accagcataa
    12781 gtgatgaaaa tatcatcagc agatttttca aggtaagccg tgtcagcaaa atcagaacct
    12841 agactttcag ataccgaatt ataaataact cctttagctt ctatattaaa atgttttaac
    12901 cactcaacgc ttctcttaga taccgcataa aaatctggac gatagtgctt aacacgcgct
    12961 gtgagaagat gttcatagat agctccaaag aaatctaaaa aacgattatt gacagaaaaa
    13021 tgacttgacc catggtctaa aacaatacta ggtaaacggt gcttctttgc aaaagatagc
    13081 ccttctagcg ttgttaactg aaaacgtgta ttacaaatca caaaatcaat attttcatct
    13141 gaaacatgtt tcatcagcgt gttgtattct cgatttttgt taataatagg atagcgctgc
    13201 ttgacaatat ttttggtcgg taaacggtaa atttttctac ccttgtcttc atctataatc
    13261 ggtaaatcat catgattagt tgttacaata acaacacggt agccacgctt aaccaaatct
    13321 gctgtcattt tatctgtata acgttcaata cctccgagga agggtagata atatcctgag
    13381 aaaacagcaa ctgtttttac cttattttcc atatttatcc actttcatca ataagccatc
    13441 ttttaagcct ttaatcatag caactaattt tttgctcttt tgctcttctg ctaccaacac
    13501 tcgaacaaat tcatttcgca taaatactaa atatttgtgc cgcttcttct taccatattt
    13561 ttttataata taaatcgcat tgcgtatcat gtaatatttt cgaaatggtg aatgattcaa
    13621 tacatgaaaa acatggccaa attttttaac tcgtgaagag tgtccaattt cgtgtaaaag
    13681 accaataaaa ttaacctgat aagtcttata tcccatctct gacagacgat aattcatttc
    13741 agagtcaaca aaatcaataa acatctcttc tgcaaagcca gatgtttctt caaaaacgct
    13801 cgttttcatt aaagcagccg aagtaataca ctcttcaatt tctttatagt caaattcttg
    13861 cataactaaa tcttcacggt tcatatcttg atacaaacaa gataacatac cgaccttagg
    13921 taaaagaagg taattttcat aattatctat caaatcacct aggacaaccg aatcttgatc
    13981 taaagtcaag aaccaatcaa attcttgtgc tactgcaaat tgaccgatac agttcaaagc
    14041 atatgcaatt cctttatttt ctgttaaata atcaacagtt aggtgcccct cttcattata
    14101 atcggccact aattgagaaa tttcttcctt atttttcgag ccattatcta cgatgtagat
    14161 atggcttact tgaggataaa ttgctcgaat gttctgatct aagcgttcaa tattggggtt
    14221 aaaggtgaca atacccgcta aatatttcat gttctatgct cttttctaaa atctctaaat
    14281 aactgaatga ctggtgcttt ggttataaaa acgataccga catagatagt tactgctact
    14341 aaactttgaa tgacataatt taccaatgat actgacattt gagtattgat ataataaagt
    14401 acagctccac taagagtagc agcaattaaa tagcgcagca ttcctcttgt taattcttta
    14461 aaagtaaata catctcttaa agagatagct tgatagaggg agacaataaa ttcagtaata
    14521 actgtagaga taatagctcc catagcacct aaaattggta ttaaaagtat attaagcaca
    14581 acatttgcca caagtccaat aactgcagac attgtgtaag cttttgtacg tcttgaagcc
    14641 agtagatact gtgtccctaa agcgttacca taagaaatgc aaatgatcat caaagactca
    14701 acagccatta gcggaccgac agcttcatac cctttcccaa aaaagaagac ggcaaatgtc
    14761 gacgaaactc ctaccactcc cgcaatcatt aacattgaga tagcattgct taaatcaaca
    14821 agtttcaaaa gtaaagcctt agcttgcttt tcttttcctg aagaaaatag actggagagc
    14881 cttggtaaga aaactcctcc gattgctgaa acaatggtaa ataaaatacg aataatttta
    14941 tcagattgat caaaatatcc agcactgacc acactatcca ttgctcccag catactcttg
    15001 ttaagagaga gataaatctg catggtgatt tggggtaaaa ataacattaa agttggtctt
    15061 agatgtatta agatatctaa tctactaaat ggtatcttga tgatttcatg ctttaaatga
    15121 tgccagactg ttaaatttcc gagtaatgat gcaaatgcaa ttaaaaacat ataaagtgct
    15181 aggtcatctg gcgtcttcac aactaagaag gtcaaaacaa ctgtaataag cttaacaatg
    15241 gtattacgaa tgacaattat cttaaagtct tcaactccaa taaaatacca cgaaatatca
    15301 gttgcagtcg ctaataaatt aataccttga attaaataga ataattgcca ttcgtttgta
    15361 agtaagacaa atccaaagaa taataacgtt gctatagata cagaggcaag ttttaataca
    15421 actaattccc aaaatatctt acttctctta tacttatttg actgcacaaa agctatctca
    15481 cgattaccgt acaaagatat ccctaaagca ccaaataaag taaagtacat gacaatggag
    15541 ctcgtatagg cattaatacc gatgccttga ggagatagta cacgggaaac ataaggaact
    15601 gttactaaag gtaacaacaa agtcaataac tggtatgagg tattataaaa catatttttc
    15661 aaaagtttca ttagtttctc ctcataatta aactaataac agtatcaaat aacaataagg
    15721 catttgatac aattaccaag ccagttaacg catacggcac cactttcctt cctgcctcat
    15781 ttagacgcgc tggaatactt gatgcaaaaa cttgtaccaa aagcaatgta acagtaaagt
    15841 aacgtccttg aactccttca atgtagcttg ttggcgctcc acgatattct aacatgataa
    15901 aaaagacacc aagaattccc acaaagaaac tagctaagaa aagaaatccc atattttttg
    15961 acttaacaaa tttttcttca gaaaaccata ctagaaagac catgagatac catactgatg
    16021 atacccaagt gggaataaaa ttctgacttg gatatggaga ggcaagccag gaagtaatgt
    16081 cattacttaa aatattgata ccagcataat tatagccaaa tgtcattaca taacgtctta
    16141 aaacagcaag gacaccacca tgtcttgcgg tcagaacata aactatagca agtgacaaaa
    16201 aggttagaca tagccaaaat agctttttag atttaagacc ttgaaataaa tgtgatagct
    16261 tctgattata gcccattaat ttaggattgt taacctgcaa taataccggt attactagca
    16321 ataaaaacag ccagtaattc tgcttacatg caacaatgac taaaagaatc aatcctataa
    16381 gagcaatact acgattttta gtcaacgatt tatcaactaa aagatttatc aaaagcgata
    16441 aaaatgctcc aaatacaata tatccagtcg agtcataaga tagactagca aatgaattta
    16501 gaactacggg actgaggaaa atagcaaagt agattaactt tccttttttc acataccgga
    16561 taatgagggc aaccaaggtg attgagacaa aggaactaaa taaccttgct actgtaatca
    16621 ttacccccat agaaggatac acaaatttgc ctaaaaataa gccaactgct ggcacaagat
    16681 gaccccaaaa aacaaagtta tgtttagaat agtttatctc acgaggcaaa tgattaccat
    16741 caacaaaatt ggctttattt aaatagtatt tttcaaatct agtaccatct ctataggagg
    16801 tcgattgtcg tctcatacct gaatcaatat tatattctcc atagtaagaa gtatcaataa
    16861 tgttatttga aatacgacca gacacagcta aatggtattg tccatcaggt tcattgaaaa
    16921 atggcattaa taggctcaat acaatagtaa tcgttaagcc ta
//
LOCUS       DATRME010000042        16530 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRME010000042 DATRME010000000
VERSION     DATRME010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16530
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            157..1233
                     /locus_tag="VBC23_001331"
     CDS             157..1233
                     /locus_tag="VBC23_001331"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6843277.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            1403..1741
                     /locus_tag="VBC23_001332"
     CDS             1403..1741
                     /locus_tag="VBC23_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843278.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            1872..3413
                     /locus_tag="VBC23_001333"
     CDS             1872..3413
                     /locus_tag="VBC23_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6843279.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            3453..4685
                     /locus_tag="VBC23_001334"
     CDS             3453..4685
                     /locus_tag="VBC23_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6843280.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            4898..5599
                     /gene="pyrF"
                     /locus_tag="VBC23_001335"
     CDS             4898..5599
                     /gene="pyrF"
                     /locus_tag="VBC23_001335"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6843281.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            5612..6241
                     /locus_tag="VBC23_001336"
     CDS             5612..6241
                     /locus_tag="VBC23_001336"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6843282.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            6253..7545
                     /locus_tag="VBC23_001337"
     CDS             6253..7545
                     /locus_tag="VBC23_001337"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6843283.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            7710..8633
                     /locus_tag="VBC23_001338"
     CDS             7710..8633
                     /locus_tag="VBC23_001338"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6843284.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            8647..9723
                     /gene="carA"
                     /locus_tag="VBC23_001339"
     CDS             8647..9723
                     /gene="carA"
                     /locus_tag="VBC23_001339"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6843285.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            9754..12936
                     /gene="carB"
                     /locus_tag="VBC23_001340"
     CDS             9754..12936
                     /gene="carB"
                     /locus_tag="VBC23_001340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6843286.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            13062..13385
                     /locus_tag="VBC23_001341"
     CDS             13062..13385
                     /locus_tag="VBC23_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843287.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            13382..13852
                     /locus_tag="VBC23_001342"
     CDS             13382..13852
                     /locus_tag="VBC23_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6843288.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            13830..16304
                     /locus_tag="VBC23_001343"
     CDS             13830..16304
                     /locus_tag="VBC23_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO6843289.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHELVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNFKAVGM
                     GLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILENQLSSYFDNVTTGFIFLI
                     LVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            16313..>16530
                     /gene="essA"
                     /locus_tag="VBC23_001344"
     CDS             16313..>16530
                     /gene="essA"
                     /locus_tag="VBC23_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO6843290.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGLKNNDIHFDTKRLESDSQK
                     NSGFQTELTKDLFKKEDLKKLDKNRQSKV"
BASE COUNT         5575 a         2656 c         3185 g         5114 t
ORIGIN      
        1 aggaatcgga tacgagattc ctttttataa ttaggttggt tagggtgact tttttcattt
       61 ggctattctt gaaagtttat aaaaatgtag tataatagtc acattaaaat gttttgaaaa
      121 tattgatgaa taacatcaac aaatagaggt cattatatgg gatataccgt tgctatcgtg
      181 ggtgctacag gtgccgtagg aacacaaatg attcgtcaat tagaacaatc gaatttacca
      241 atagaacaag tgaaactttt atcatcaagt cgctcagcag gtaaaatttt acattttaaa
      301 gatgaggcta tacgtgttga agagacaaca aaagaatcat tttacgatgt tgatattgcc
      361 ttgttttcag ctggtggatc tatttcagca aagttcgctc cttatgcagt aaagtctgga
      421 gcagttgtag tagataacac gtcatatttt cgtcagaatc ctgatgttcc actagttgtt
      481 cctgaagtaa atgctcatgc catgattggt cataatggta tcatagcttg tcccaattgt
      541 tctactattc aaatgatgat tgctttagag cccattcgtc aaaaatgggg gatagagcgt
      601 gttatagttt ccacctatca agctgtttcg ggttcaggtg cacgtgctgt tgaagaaact
      661 aaggaacagt tgagacaagt tttaaatgat aacctttcac ctgatcaatt aattgcaacg
      721 gtcttgccat gttctagcga tcaaaaacat tatcctatcg cattcaatgc attacctcaa
      781 attgatattt ttactgataa tgattatacg tacgaagaaa tgaaaatgac tttggagaca
      841 aagaaaatta tggaagatgc tactattaag gtttcagcta cctgcgtccg cattcccgtt
      901 ttatcgggtc attctgagtc tatttatatt gagacgaaag aattagcctc aatctctgaa
      961 attaaaaagg ctattgcaaa ttttcctggt gccgtcctac aagaccttcc atctcagcaa
     1021 atttaccctc aagcgattaa tgctgtaggc catagggaga cctttgttgg tcgtattcga
     1081 aaagaccttg atcaagaaaa tggtgttcat atgtgggtag tttcagataa tctactgaag
     1141 ggggcagctt ggaattctgt tcaaatcgct gaaacattgc ataagaatgg gctggttaaa
     1201 ccagcgaaag agttaaaatt tgaattaata tagaagaata tgacattgct taatacttat
     1261 actgacttct agtgatatta atttttcatc aatatcaggt aaaacacttg acaaattatg
     1321 aaaacatttg cataatgaaa ttaacaaatg aaaatgattg caaatcgttt taacattgtt
     1381 tccacattaa ggaggggctc ttatgcaaat catcaaacgt gatggacaga ttgttgaatt
     1441 tgatcctgaa aaaatctacc aagctatctt gaaagctgct caaacagttt atgtgcttga
     1501 tgagtcactt cgtcaagacc ttgctcaagt gactaaaaaa gtaatcttag atttagagga
     1561 tgctcaggtt gaacgaccaa cgatcaatat gattcagtct ttggttgaaa atcgtttatt
     1621 ggatgcagga tacattcata ttgctgaaca ctatatttca taccgtttgc aacgtgattt
     1681 agagcgaaat ggttatgatg atcaaatcac tgttcattta cactttgaaa aaattaagta
     1741 aggtttgact tttaagttta aactctctat acttgtatat ggaacttact atgtaatgtg
     1801 ctaatttaaa aacttgtaga gtgtctctat gagtttttta ataacctcta ttatcaatca
     1861 aaggatttta aatgagtatt ttagaagtaa aaaacttaag tcacggtttt ggtgatcgtg
     1921 ctatttttga gaatgtttct tttcgccttt taaaaggtga acatatagga cttattggtg
     1981 ctaatggtga aggaaaatca acattcatgt caattgttac aggtcatctt caacctgatg
     2041 aaggaaaaat tgaatggtca aaatatgtaa ctgctggtta ccttgatcaa catactgtgt
     2101 tagaagaagg tcaaacagtt cgtgatgttt tgcgaacagc atttgatgaa ctttttaaaa
     2161 cagaggcacg tatcaatgat atatatatat ccatggcaga tgatggcgca gatgtagata
     2221 gccttatgga agaagttgga gaactacaag atcgtttaga atcacgtgat ttttatacgc
     2281 ttgatgctaa aattgatgaa gtcgcacggg cattaggagt tatggatttt ggtatggatt
     2341 ctgatgttac tgaattatct ggtggtcaaa ggacaaaggt ccttttagca aaactgttat
     2401 tagaaaagcc agatatcctc ctgcttgatg agcctacaaa ctaccttgat gctgaacaca
     2461 ttgattggtt aaaacgctat ttacaaaatt atgaaaatgc ttttgtttta atctcacacg
     2521 atattccatt tttgaacgat gttatcaata ttgtctatca tgttgaaaat caagatttag
     2581 taagatattc cggtgattat actaattttg aatcagtcta tgcaatgaaa aaggctcaat
     2641 tagaagcagc ctatgaacgt caacaaaagg aaatagctga tttacaagat tttgttaatc
     2701 gtaataaagc acgcgttgca acacgtaata tggcaatgtc tcgtcaaaag aaactagaca
     2761 aaatggatat tattgagtta caagcagaga aaccaaagcc aagttttgaa tttaaagaat
     2821 ctcgcacacc aggacgtttt attttccaag ctaaagatct tcaaattgga tatgatcgtg
     2881 ctttaacaaa acctctaaac ctaacatttg aacgtaatca aaaaatagct attgttggag
     2941 caaatgggat aggaaaaaca acattactta agagtttact aggtattatc ccccctattt
     3001 ctggtaatgt tgaacgaggt gattttattg atttaggtta ttttgaacaa gaagtacccg
     3061 gagggaatcg ccaaactcca cttgaagcag tatgggatgc ttttcctgct ttgaaccaag
     3121 cagaggttag ggcagctctt gctcgttgtg gtttaacttc aaagcatatt gaaagtcaga
     3181 ttcaggtgtt atctggtggg gagcaatcaa aggtcagatt ttgcttatta atgaatcgcg
     3241 aaaataatgt ccttgtactt gatgaaccaa ccaatcattt agatgtagat gctaaagatg
     3301 agttaaaacg tgctttaaaa gcttataagg gatcaatctt gatggtatgt catgaaccgg
     3361 acttttatga aggatggatg gatgatgttt gggactttaa ccaacttagt taagtggcta
     3421 gggtaataaa gtagaaggca gagccaaata agatgcaaaa tgatatgagc gttgttctta
     3481 gagctggtaa attactgatt gaaagcggag cagaagtcta tcgtgtcgaa gacacaatga
     3541 agcattttgc taaggctctt cagatagaaa attttgaagc ttatgtggtt agtagcagta
     3601 ttatcgcttc cgggattaac cgctatggta aacaagaagc taaagtatgc aatacagatg
     3661 gtgtaacagc aaatttaggt agacttgaag ctgttaataa cctttctagg caaattgcta
     3721 aacaagactt agtatctcca gaagaaattg taaagcagtt agacttaatt gaacatcaaa
     3781 aagattatag tttgcttgtt actttaattt cttatttttg tggagcgggt agtttttctt
     3841 tagccttggg tagttcctta ttagattcat tttccgctgc tgtaacaggc ttaattttag
     3901 gatatttttt gaatttgatg gaaagtcgta tacatacagg atttcttttg accattctag
     3961 gtagttctgt agtggcttta agtgctaatt tattatattt ttcaggatta ggtgagcatc
     4021 gtagcattat catattaggg gcattaatgg ttatggtgcc aggggctgcc tttgttaatt
     4081 cggtgagaga attttcacag aataattttt caacaggttt agctttgatt atgtctgctt
     4141 tactcatttg tatttctata tcagcaggcg tggctattac aattgaaatt attccgtttg
     4201 cagaacaaat gacaggttct ttttctggtg ttcctaatac cattcttgag attatcatca
     4261 gaacactaat ggccggttta gggacgatag ccttctctat tctctaccat gttccaaaac
     4321 gttatttcct tgacttaggg atactaggag caatatcttg gatgctctac ctgatcttat
     4381 ggcaacaatt tcacatggat gctatagccg tattttttcc agggcttttc atcacttatt
     4441 tttcacggct attagctgct aaacgtaagt gtcctgccac tgtcttttta gcgacaagta
     4501 tgtttcctct tatcccagga cttagcttct atagagccgt ttacttttta ttgacgggag
     4561 cagatgcctt agcaatggaa tattttagat cctgttttgt aacagctttt acaattgcta
     4621 tagcaatcag tattgtacag cagattcctc tatcgttctt tattagacgt aaaatgataa
     4681 aataatttag tatttatttg acaaaatgat tgtaaacttg tagaatacct acaaatgtat
     4741 ctttaagtga gtccagagag gcgaacaagg tcaccatcat aagtcaaata gcgagtgctt
     4801 tttcgctaac tttttatgaa accttgccct caggcaaggt tttttgtgtc atactggtac
     4861 atttccaaca attattattt ctatagagag gaattatatg ttagaaaaat gccctatcat
     4921 tgccttagat ttttctgatt tggcttctgt aacaactttt ttagaacatt ttccaaaaga
     4981 agaattgctt tttgtcaaaa taggaatgga gctttattat tccgaaggtc cctcaattat
     5041 tagatatatt aaatccttag gacatcgcat ttttttggat ttaaaattac atgatatccc
     5101 taatacagtt cgattatcta tgtctgtctt agccaaatta ggcattgata tgactaatgt
     5161 ccatgctgca ggaggagtcg aaatgatgaa agcagctaga gaaggtttgg gggagggtcc
     5221 aatactatta gcagtgacac aattaacctc gacttcacaa gaacaaatgc aggtggatca
     5281 acatattaac ttaagtgtag ttgattcggt ttgccattat gctcaaaaag ctcaagaagc
     5341 aggcttagat ggagtagtcg catcggctca agaggtaaag caaatcaaga aacagactaa
     5401 cgaacatttc atttgcttaa cgcctggtat cagacctcca cagaccaatc agttagatga
     5461 tcaaaaaaga acgatgactc ctgagcaagc acgtatagtt ggggctgatt atatcgttgt
     5521 gggaagacct ataacgaaag cagaaaatcc atatcaagca tatctagaca taaaagaaga
     5581 atggaatcgt attaaataaa ggagtactga gatggattta gcaagacaga ttgccatgga
     5641 actattagat attcaagctg tttacttaag accccaacaa ccctttactt gggcttcggg
     5701 ggtcaaatcg ccaatttaca ctgataatcg tgtaacctta tcgtatcctg agacaaggac
     5761 actgattgaa aatggttttg ttaaacaaat acagaaacat ttcccaaacg ttgatatcat
     5821 cgcaggaaca gcgactgcag gcataccaca tggcgctatt atagctgata agatgaactt
     5881 gccctttgcc tatatacgct cgaaggcaaa ggatcatggt gtggggaatc aaatcgaggg
     5941 acgtgtttac tcaggccaaa aaatggttat cattgaagat ttgatttcaa caggaggatc
     6001 tgttttagag gctgttacgg ctgcgcagag tcaaggtatt gaggttttag gggtggtagc
     6061 gatatttaca tatcaattag ctaaggctga acaagcattt cgtgaagctg atattccttt
     6121 ggtgactttg acggattaca atcaattaat taaagttgct aaagttaatg gatatattac
     6181 agctgaccaa ctggtattac tgaaaaaatt taaagaagat caaatgaatt ggcaaagtta
     6241 gagaggacag atatgtatat cattaaaaac ggattgatta tcgaccctca aagtggtttt
     6301 aatcaagtat cagacatgct gattgatcaa ggaaaaatta aacaaatatc gaaagaaatt
     6361 gatattaaag gcattccaat cattgatgca agcaataaaa ttgtagctcc aggattagtc
     6421 gatattcatg tacattttcg tgagcctgga cagacacata aagaaaatat ccatacagga
     6481 gccttatcag ctgcagcagg tggttttacg acagttctta tgatggccaa tactaaccca
     6541 acgatttcta gctcagaaat tgtaaaacaa gttaaggaga gtgcagcaaa agaagcaatt
     6601 aagattgaaa cagttgctac aattactaaa tccctgaatg gtaaagattt agttaatttc
     6661 gaagaattat tagaagctgg agttgctgga ttctcagacg atggtatccc tttgacagat
     6721 actaaggttt tgcaagaagc tatgaactta gctagaaaac atgatgttgt cctatcgtta
     6781 catgaagagg atccatcgtt aaatggtgtt cttggtatca acgaacatat tgctcagaaa
     6841 atttatcatg tctgtggagc tagtggtctt gctgaatatt caatgattgc gcgagatgct
     6901 atgattgctt accaaacaca ggcgaaagtc catatccagc atttgtcaag ttcagaatca
     6961 gtagaagtag ttgattttgc gcagaagctt ggtgctaact taacagcaga agtaaccccc
     7021 caacattttt caaagacaga ggacttactt ttaactaagg gtgctaatgc aaaattaaat
     7081 ccaccactgc gacttgaaaa ggatagacaa gccctcattg atggtcttaa aagtggtgtt
     7141 atttcaatta ttgcaagtga tcacgctccc catcatatta tggagaaagc agcagataat
     7201 attagtcaag cgccctctgg tatgactggc ttagagacat cattagcctt agggattact
     7261 tatttagtat ctactaaaga actctcaatg attgactttt tggcaaaaat gacatgtaat
     7321 cctgcacagt tatatggatt tgatgcaggt tatctcagag aaggtggacc tgcagatatc
     7381 gttatttttg atcaagctga agagagaata ataaaggcag aatttgcctc caaatccagc
     7441 aactctcctt ttatcggtga taaattgaag ggagttgttc actacacaat ttgcaatggt
     7501 gaaatagtct atcaaaaaga tagccacgca gcgacattgg tataggtaaa acatttctta
     7561 ttaatatcct ttaaggtatt gtccagagag gcaaacaagg aattgcgtta acaaatagta
     7621 agttagttcc ctatctagct tactattttg cagagcctcc ttgttttagg aggctttttt
     7681 ctagctaaaa tttatagaaa gaaattatca tgaatcatca aacacaaaca ctttcattag
     7741 aacattttgt cagtttagaa gaattatcaa atcaagaagt tatgtctttg ataaagagaa
     7801 gtatcgaagt taaagaaaat cctagcaaca ttggttttga taaagattac tatgtgtcaa
     7861 atctcttttt tgagaattca acaagaacac ataagtcatt tgaaatggca gaactcaaat
     7921 taggattaaa aacaattgaa tttaatgctg atacaagttc tgttaataaa ggagaaaccc
     7981 tttatgacac tatcttaaca atgagtgctc taggattgga cgtttgtgtc attaggcacc
     8041 ctgatattga ctattataaa gaattgattg ctagtccgaa cattcattca gctattgtca
     8101 atgggggtga tggctcaggc caacatccaa gccaatcact tttggatttg gtaactattt
     8161 acgaagaatt tggatacttc aaaggactaa aaattgcgat tgtaggagat ctaactcact
     8221 cacgggtagc taaatctaac atgcaagtgc taaaacgatt aggagctgaa attttctttt
     8281 ctggacccaa agaatggtat agtagtcagt tcgatgaata tgggcaatgt ttaccaatag
     8341 atcagttagt tgatcaaatt gatgtcctta tgcttttacg tgttcaacac gaacgtcatg
     8401 atggtaaagg ggtgttttca aaagaaagct atcatcaaca atttggtctt acaaaagaac
     8461 gctataaaca tttaagggat acagcaatta ttatgcaccc agcacccgtc aatagggatg
     8521 tagagattgc tagcgattta gtagaagcag ataaggctcg aatagtgaaa cagatgagta
     8581 atggcgtcta tgcaaggata gctatcttag aagcagtgct caatagcaga taataaagga
     8641 gataaaatga agcgtctatt attattggaa gatggtagtg tgtttgaggg tgaggctttc
     8701 ggagcggatg taggaacaag tggtgaaatt gtttttagta caggaatgac agggtatcaa
     8761 gaatctataa cagatcaatc ttacaacggt caaatcatta cttttactta tcctcttatt
     8821 ggaaattatg ggataaatcg agacgattac gaatctatta gaccaacatg taagggagta
     8881 attgtttatg aatgggcaga gtatccaagt aactggcgtc aacagatgac atttgatgaa
     8941 tttttaaaat taaaaggaat acctggaatt tcaggtatag atactcgagc attaacaaaa
     9001 attattcgaa aacatggaac aatgaaagct tgtttaataa acgagggaaa ttctattcat
     9061 gaagcactag aaaaccttca aaaaagtgtt cttttaaata atcaaattga acaagtatca
     9121 accaaattag cttatgcttc gcctggagtt ggcaaaaata ttgtattagt tgactttggt
     9181 ttaaaacatt ctattctaag agaactgtca caacgtcaat gtcatattac agtcgtccct
     9241 catactacga cagcgcaaga aatcttaaat ctcaatccag atggagtact cttatctaac
     9301 ggccctggga acccagaaca attaccgaat gctttacaaa tgatccaaga aattcaaggt
     9361 aaaattccaa tttttggtat ttgtatggga catcaactat ttgctaaagc caatggtgca
     9421 aagacttata aaatgacttt tggtcatcga ggttttaacc atgctgttcg tcatttgcaa
     9481 acaggacagg ttgattttac aagtcaaaat catggttatg ctgtctcgag agaagatttc
     9541 cctgaggctc tcttcattac acatgaagag attaatgata aaaccgtcga aggtgttcga
     9601 cataaatact atcctgcctt ttcagtgcag tttcacccag atgcagcacc agggccgcat
     9661 gatacatctt atctttttga tgaatttatt aatatgattg atgatttcca gcaaaaaagc
     9721 tagctttaaa atatagaatt agaggagttt gatatgccaa agcgcacaga catacgtaaa
     9781 attatggtta tcggttctgg cccgattgtg attggacaag cagcagagtt tgactattct
     9841 ggtacacaag cctgtctctc tttaaaagag gagggatacc aagttgtttt ggtcaattcg
     9901 aatccggcta ctatcatgac agataaagat attgctgata aagtttatat tgaacctatt
     9961 accttggagt ttgtcacaag aattctcagg aaggaaagac ctgatgctct ccttccaact
    10021 cttggaggtc aaactggatt gaatatggct atggctctat caaaaaatgg tatcctagaa
    10081 gaattgaatg ttgaactatt agggaccaaa ttatcagcca ttgataaagc tgaagatcgt
    10141 gatttattta aacagttgat ggaagaactc aatcaaccta ttccagaatc cgagattgtt
    10201 aattcggtag aagaagctat tcaatttgca gagcaaatcg ggtatccatt aattgttcgt
    10261 cctgccttta ctttaggagg tacaggtgga ggcatgtgcg ataatcaaga acaattggtt
    10321 gacatcacga cgaaggggtt aaagttatcg cctgtgacac agtgccttat tgaacgttca
    10381 attgctggct ttaaagagat tgaatacgaa gttatgcgtg atgcagcaga taacgctctt
    10441 gttgtttgta atatggagaa ttttgatcct gttggtatcc atacagggga ctctatagtt
    10501 tttgcacccg cacaaacgtt atctgatgtg gaaaatcagt tattacgaga tgcgagttta
    10561 gatattataa gagctttgaa aattgaagga ggatgcaatg ttcaactagc ccttgatccg
    10621 aatagtttta aatactatgt cattgaagtt aatccaagag tatcaagatc ttctgcttta
    10681 gcttcaaaag ctactggata tccaattgct aaattagcag ctaagatagc tgttggtttg
    10741 acactagatg aggtaattaa tcctattaca aaaacgactt acgctatgtt tgaacctgct
    10801 ttagattacg tagtcgcaaa aatgccgaga tttccttttg ataaatttga aagtggcgat
    10861 agaaaattgg gaacacagat gaaagctaca ggcgaagtca tggcaatagg acggaatata
    10921 gaagaatctt tgttgaaagc ttgtcgttca ctagaaatag gtgtcgatca cattaaaata
    10981 gcagatttag ataatgtatc agatgatgtt ttattagaaa aaataagaaa ggcagaagat
    11041 gatcgcttat tttatctagc agaagcttta cgtagacatt atagtattga aaaattagca
    11101 agtttgacta gcattgattc tttcttcctt gataagttaa gagtgattgt agagttagaa
    11161 gatctattat ctaaaaacag actggatatc aatattctca aaaaggttaa aaataaaggt
    11221 ttttcagata aagctatagc gagtttatgg caaataaacg aagatcaagt tcgtaacatg
    11281 cgaaaagaag caggaattct tccagtttat aagatggttg atacatgtgc agctgagttt
    11341 gattcagcaa cgccctactt ttattcgact tatgccgtag aaaacgagtc attgatatca
    11401 gataaagcct ctattttggt tttaggatcg ggcccaatcc gaattggaca gggagttgaa
    11461 tttgattatt caacggttca ttctgttaaa gccatcagag agtctggttt tgaagcgatt
    11521 atcatgaact ctaatccaga gacggtctca acggatttct ctatttcaga taagctttac
    11581 tttgaacctt tgacttttga agatgttatg aatgttattg acttagaaaa acctgaaggg
    11641 gtcatcttac aattcggtgg tcagactgct attaatctag caaaagattt aaacaaggca
    11701 ggggttaaaa tactaggcac acaattagaa gatttggatc gtgctgaaaa tagaaaacaa
    11761 tttgaagcca ctttacaagc tctaaatatc cctcaaccac caggttttac cgccactacg
    11821 gaggaagagg cagttaatgc agcgcaaaag attggttatc ccgtgttggt tagaccatca
    11881 tatgttcttg gtggacgagc tatgaagata gttgaaaacg aagaagattt acgtcattat
    11941 atgacaaccg cggtaaaggc aagcccagat catccagtgc ttatagatgc ataccttata
    12001 ggtaaagagt gtgaggttga tgctatatcc gatggtcaga atatactgat tcctggaata
    12061 atggaacata tagagcgagc tggtgttcat tcaggagatt ccatggctgt ataccctcct
    12121 caaacattgt ctgagaccat tatcgaaact attgtagatt atacaaaacg cttagcaatt
    12181 ggattaaact gtatcggtat gatgaatatt cagtttgtaa tcaaggatca gaaagtatat
    12241 gttattgaag ttaatcctcg tgctggtaga acacttccat tcttatcaaa agtaactcac
    12301 attcctatgg cccaagtagc aacaaaagtt atcttaggag ataaactctg caattttaca
    12361 tatggttatg atctctatcc agcttcagat atggtccaca ttaaagcccc tgtctttagt
    12421 tttaccaaac ttgctaaagt agatagttta ttaggtcctg aaatgaaatc aacaggcgaa
    12481 gtgatgggat cagatattaa tcttcagaaa gccttgtata aagcctttga agcagcatat
    12541 ctccatatgc cagactatgg taacattgtt tttacagtag atgatacaga caaagaagaa
    12601 gctttagaac ttgcaaaagt ctatcaaagt attggttatc gtatttatgc tacccaaggg
    12661 actgcaattt attttgatgc taatggttta gagacagttt tagtaggtaa gttaggagag
    12721 aatgatcgaa atcatattcc tgatttaatt aaaaatggta agattcaagc agttatcaac
    12781 acagttgggc aaaataacat tgataaccat gatgctctca tcatcagacg ttctgcaatt
    12841 gaacaaggag ttcctctatt tacatcttta gatactgcac atgcaatgtt caaagttctt
    12901 gaaagcaaag catttacatt aaaagtacta gattaatcaa acaatcaagg taaaattaac
    12961 ttccagcaaa acagaatcgg actagattaa atatagtttg ttctgttttt ataaaaaagc
    13021 ctaattgtga taaaataggg aacaaactta aggagttaat tatgttaaaa acacgaacac
    13081 atattaatga attagaaaca aaaatcgccc ttgatttagt acacagatat ctaggtattc
    13141 aacaacttgt tttttcaaag tggagacatt ctttagaaac tggttcatgg tcttctatta
    13201 taacaataaa tggcgctaat attatcaatt acattgttga tgatattagc gacagtgata
    13261 gtatttcagg tattatttgt catcctaaaa gttttcatct cagacctaga gaaaaaaagg
    13321 aacctattca gtctattgat gagttagata tcatctggtc tttgaataga gaggaaaatg
    13381 aatgattcca gatagtagtt ttaaagtgct atcagatatg gcatataata ttgaaaaatc
    13441 caaagcagaa agtaatggac accttaaaat tatagaaggt agtcaaatta ttgatttcca
    13501 tcaaaaacgc tcttttatgg tattagaggt taatgataat atcgataatg gtatgcaggc
    13561 tatggcatgt cagtctttgg atgagagtaa tcatattgtt atagcatatt caggaacgaa
    13621 cgcttttaat aagaaagata tcttaacaga tatcgattca gttgtattgg ggaagaacaa
    13681 taaattgcta acttgtcctt caagtattac cctgagagag gatggcattt ctctgccttg
    13741 taaaaatcca agtcaaatta caacatctaa actatttgca caaaaaatta agatgaacta
    13801 tcctgatgca accctctggt taacaggcca tggtaaatgt tcagtcaggt aatatctcaa
    13861 attaccaaaa aaatctttta gattctgcaa ctaatttcca aaatatcttt ccagccctcg
    13921 ttaaccagta tagtagttcc attactgcta acgaatcact gaaaaaatct ttagaagctt
    13981 ctgataacat gtttaatgat ttgatgacaa cccagacaaa tactggaaaa gatttatcaa
    14041 gcttaataga acagcgccat caagatagca tttcgtatga agcgttttcg gcctcattac
    14101 tagaaatgaa taacgagtta ttagagaagc aattatctga tattatcaca caagcacaaa
    14161 aagaccaaga aacattatca tcacaactca atagtattat gggtgatgat aacgataatg
    14221 atcataatca taaagaaaac tcatcagctt atctaaatgt tgcaaggcaa aaaatccaag
    14281 aactatctga agcactcaag tcacaagata acatcgctaa agatcaaagt gaacaactag
    14341 ataaaattgt tagagaggga ctagcaagtt actttgctaa gaataataaa gataatatta
    14401 ctttattaga attattgaag agtcattcta ctaatgagaa gactttgaaa gattttaaag
    14461 ttaaggtagc agatttcaca aattctctca tttcaagcat tccctcacta aacctttcgg
    14521 agttgcactt aacccaagaa gaaaaagcca ttcagtttac atcttctgat tcggaaatca
    14581 ttaaaaaagt aagcaatgaa agaactctgt atttaaatac taatttattg aatcgtcttt
    14641 ttgaagctag gaaaaataga gatgaagcca aaaataaagt taatcaatta aaattgccat
    14701 ctagttctac tcgaactggt gagcagatag tttctgttga atcaaataat ccagattaca
    14761 gggtagatac ttggacggtt aatggtaagc aaacaagaac cttggacccc agccaaaaca
    14821 ataatattat tatcaatagc agttatcaag gggaattatc taactctagt attacaaaac
    14881 caagctatac aatcactatc gggaatcaga aacaaattgt acaaaatgat gacggtgaaa
    14941 ttcaacggtc ttacttggag gcagaagcaa cgtatcaacg aactctacaa gaagtaattg
    15001 atgcatataa caccactcac gaattagtag ctaaatacta cattatttct gatggcgaag
    15061 aaccgcaaaa tttatttgat caatttttaa atcaaagtgt taacgatacg atggttgatc
    15121 tggtcaaaaa tggtataact aagtatttga tggatgaaaa cactgctgat gcgcaacaga
    15181 aagttaaaga tgtcatggag gaagttgaaa atagccaaga tgaattggcg gatcaaatgg
    15241 caaaagtaac tgagacaaat gtacgactaa cagatacaat caaaaaacag cttgagactc
    15301 ttcaaacaat taacatgaag gtccaaaaca tcacacagga tcaatctaaa gtaaacgact
    15361 cacagaaaac aactgatcaa caattatctg atttgaagaa ccagctagat gggctaatga
    15421 cctctgcaac tggcgtaaaa gatatgtcca aatctaatag tcaagaggcg gaccaagtta
    15481 atcaaatatt cacatcattc aataaagatg ttcaagatgc taaaaattcg ggcaacaaac
    15541 tttcgacgga tgcaacagat ttaatggcta acttccaaaa agaattggcc aataacggtg
    15601 atttcgtagc ttctttctct aaagttttta atactgctta taaaaatgga gtgccaaatg
    15661 atattctcct taatttctta tcacgacctg tcgctgaatc agcgtctgca gttagggcaa
    15721 ctgaaaatac ttatcgccca ttcacttgga tactattatt agaagttgtt agcctattta
    15781 tagcatatat ctttgcgact caaaacctta ttaagaaatt gacagatagg tataatgtca
    15841 atcgctggct tcaaacagac tttttaaata tcattgtcat ttcaggcctc tctcttgtga
    15901 ttggtttagc actgggagtt atctcaagca gaagtcttca tgtcatgcct gaatatgtac
    15961 catcttggtt cctagttatg actctgttta gttttttatt aattcatagt cagtatttct
    16021 ttattaaaaa ttttaaagca gttggtatgg gtttagcttt gtttatgatt attagttttg
    16081 tgtatctatc aaatgccgta ggcacagtag cgactgttag tggatttcca aaattactaa
    16141 aagctattaa tccactatct atccttgaaa atcaattatc atcttatttt gataatgtaa
    16201 caactggatt tattttcctt atcttagtac ttcttgtgga tgttgctttt attatcatga
    16261 atatttttat caccttaaat tttgaagcta aagtaaaaga gtgaggttgt tgatgaagtt
    16321 gaaacgattt ttagcctgtc tactaatatt atcctgtatg agtcaaacat cagtattttc
    16381 tgatgggtta aagaataatg atattcattt tgatacaaaa cgtttagaat cagattcaca
    16441 aaaaaattct ggttttcaaa ctgaattaac taaagattta tttaaaaaag aagaccttaa
    16501 aaaattggat aaaaaccgtc aatctaaagt
//
LOCUS       DATRME010000043        16478 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRME010000043 DATRME010000000
VERSION     DATRME010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16478
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(19..948)
                     /locus_tag="VBC23_001345"
     CDS             complement(19..948)
                     /locus_tag="VBC23_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843291.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(932..1624)
                     /gene="ftsE"
                     /locus_tag="VBC23_001346"
     CDS             complement(932..1624)
                     /gene="ftsE"
                     /locus_tag="VBC23_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6843292.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            complement(1643..2741)
                     /gene="prfB"
                     /locus_tag="VBC23_001347"
     CDS             complement(join(1643..2668,2670..2741))
                     /gene="prfB"
                     /locus_tag="VBC23_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6843293.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            complement(2810..3934)
                     /gene="queG"
                     /locus_tag="VBC23_001348"
     CDS             complement(2810..3934)
                     /gene="queG"
                     /locus_tag="VBC23_001348"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6843294.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            complement(4024..5955)
                     /locus_tag="VBC23_001349"
     CDS             complement(4024..5955)
                     /locus_tag="VBC23_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO6843295.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            6107..6967
                     /locus_tag="VBC23_001350"
                     /pseudo
     CDS             6107..6967
                     /locus_tag="VBC23_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(7012..9696)
                     /locus_tag="VBC23_001351"
     CDS             complement(7012..9696)
                     /locus_tag="VBC23_001351"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6843296.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(10040..10438)
                     /locus_tag="VBC23_001352"
     CDS             complement(10040..10438)
                     /locus_tag="VBC23_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6843297.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            10502..11818
                     /locus_tag="VBC23_001353"
     CDS             10502..11818
                     /locus_tag="VBC23_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6843298.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            11890..12702
                     /gene="yidA"
                     /locus_tag="VBC23_001354"
     CDS             11890..12702
                     /gene="yidA"
                     /locus_tag="VBC23_001354"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6843299.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            12687..13917
                     /locus_tag="VBC23_001355"
                     /pseudo
     CDS             12687..13917
                     /locus_tag="VBC23_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
     gene            13930..15141
                     /locus_tag="VBC23_001356"
     CDS             13930..15141
                     /locus_tag="VBC23_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6843300.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            15160..16395
                     /locus_tag="VBC23_001357"
     CDS             15160..16395
                     /locus_tag="VBC23_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6843301.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
BASE COUNT         5414 a         3235 c         2624 g         5205 t
ORIGIN      
        1 agatttcatc tctttttatt aaatcttaag gtaacgtctc attgatagga cagatccaag
       61 tgagccaata ataataccaa taatcaccat accaccaata attgctggaa ccataatatc
      121 cataggatac aatgaaacat ggcttgtctc aaattttgga ttaaacttgt taaacacaaa
      181 ttgataacca aaatagaaaa tcagcgatgg aacgatagcc cctaaaatcc ctacccaagc
      241 accttcaaaa aagaacggtc ctcgaatata agaattttta gcgccaacta atctcatgat
      301 ttcaatatct gtacggcgtg acataatcga catacgaata gtatttgaaa tcaaaaagat
      361 agcaaggaca attaataagg caacaccacc aaatccccat atacggatgt tatctgtgag
      421 attagatagt tttgttgaat caataggttc tttagtataa tcaacacctt caatgcgtcc
      481 aattgcttca ctgacactct tagaaacttt tgcagaactt gtcccaacta cgtaaacatc
      541 taacaaagca tctttgtagg tatcatcaat aacatctgaa ccatactctt tttgaacttc
      601 ttttaattgt tcagctttac ttgaataagt gactttctct acaccagaaa tacgtttaat
      661 cttgtcataa actttatgat aatcaggatt atcttttagc tttccgtcct tatctttgac
      721 ttgtttttga gcatccgttg agtcaacatt taaaaaggca ctgatagtaa aattacctga
      781 aacatcagtt gttaactttt caacattcag taaaactgat gaaaacaagc ctacaagaag
      841 taatgttatg gtaactgatg ttacagaagc aaaagtcatc caaaagttac gttttaagtt
      901 tttaagcgat tcccagaaat gtctaaagaa attaatcatg ataaccatat tctcccttct
      961 cttcatctcg gataacgcta cctgcttcaa tctcaatgac acgatgacga agtgtgttaa
     1021 caatttgact attgtgtgtt gccatcaaaa cagtcgttcc ttgaaggtta atacgttcca
     1081 atagatgcat gatttcccaa gcgatttctg gatccaaatt accagtaggt tcatctgcaa
     1141 ttaataattt tggattatta acaatcgcac gtgcaattgc cacacgttgt tgttctccac
     1201 cagataactg tgttggaaaa gaacgcatct tgtgttttaa acctactaat tctaaaactt
     1261 ctggcacgcg ttttttaata tgacggcgct tagctccaat aacttgcatc gcaaacgcaa
     1321 cattctcata aactgttttt gttggtagta atttataatc ttggaagaca actccaatac
     1381 tacgacgtaa aataggaatt tgacgacgtt tgagtttatt gagattgaat tcccctacct
     1441 ttaatctacc tgaacttaat ttttcttcac ggtataggag gcgaatcaag ctagatttac
     1501 cagcacctga cggaccaact aaatacacaa actcaccttg ctgaattgat aaatttaagt
     1561 ttctcaaggc agttgttgaa cggcgatact ttttagtaac accagacatt tctattagtg
     1621 ccatgtttct ccttcattat ttttagtctt caatgcgcca cttaaggtag gcatcaataa
     1681 agccattgat ttctccatcc ataaccttat ctacctgagc aagctcaaag ttagtacgat
     1741 gatccttcac catcgtataa ggggtgaaaa cataagaacg aatctgactt ccccaagtga
     1801 tttctttttt atcacccttg agagcatcta cttcttgtgc tttcttttct tgttctaact
     1861 gatatagctt agcctgcaac atcttcatag cgcgatcgcg gttcccatat tgagtacggt
     1921 caacggtaga cgataccaca attccagttg gaatatgcgt caaacgaaca cctgttgata
     1981 ctttattgac gttctgacca ccagctcctc cggaacggaa agtatccatt ttaatgtcat
     2041 catcacgtac ttctacttca atcgtatcat ctaactctgg catcacttca actgaagcaa
     2101 atgaggtatg acgacgttta gccgaatcaa aaggtgatat acgaacgagg cgatgaactc
     2161 ccatttctga tttcaacaaa ccataagcat tgggaccttc aaaagagagg gtaaccgatt
     2221 taataccagc ttcatcccct gcttgataat ccaatacttc tactttgaaa ccattagcat
     2281 tgccaaaacg ggtatacatt cgtaagagta aatctcccca atcttgcgct tcagttcctc
     2341 ctgaaccagg atggatttct aagatagcat tattgtggtc ataaggctca gataagagaa
     2401 gcgtcatttc ataagcaccc attatcttat ctagctgcat tagattttct tctaattctt
     2461 ctttcaaaga atcatcttcg tcaagcatct caagtaagag ttctgtttcg tcagataatt
     2521 cttgcatatt atggaaagta tcatacttac cttttaactc attcagttct tgagatgtct
     2581 tttgcgctgc aatattgtca ttccaaaagt ctggctcagt catttgattt tcaagaagag
     2641 caatctcctc ttccaaacga tctaagtcaa agagacctcc tgaagctagt caacttctct
     2701 ttattttcta ctattttttg gcgaatttca gccacttcca tgagatttct ccaatctttg
     2761 ttaatctgtt ttattttatc ataaggttct taaaaatacc ataaaaggct taaaattgcc
     2821 atttatgacg gattaattcc aactcttttc ggctatcttc atcttttaag gttaaattac
     2881 tcataaactc cagtatctca tcgtttggtt ttttgacaat ttctcccaat gcccatatag
     2941 ctgttgcagt atgaataggg ttattatttt tatcaataat ctctattaat ttaaccactg
     3001 cggttttatc atgagcattt gctagcgcaa taatggcatt acgctgtaaa atatttttac
     3061 ctcgccaact tccagcaatc ataccaaatt tttctttaaa ttgtccattt gataaactca
     3121 aaaatggtat taattctggt tgagccaact caggatcaat ctctgtcgcc aatggattat
     3181 ttataccttt gttataagga cagcaaattt ggcaaatatc gcaaccatag ataactgttt
     3241 taatcttttt tcgaaactct atgtccatca ttcccttatc ttgggtttga aatgatagac
     3301 aacgcttggc attcattgag ccatcaccaa ttaaacaaga agttggacag gcatctagac
     3361 aacgacgaca gtcaccacaa tcataatcga caggcttgtc tggctcgatc tctaaattgg
     3421 taattaattc tcccaaaaac atataagagc catattcttt agaaataact agaccatttt
     3481 ttcctataaa accaatacct gctctttggg caactgcagt atcaactaaa gctccagtat
     3541 cgaccattgc tttttgttgc aaaggaaaat ctcgacagag ctcttcaatg ccttttgata
     3601 gtctgtctaa cttttcgccg accacataat gataatcaag cccccacgaa ttaggtgtaa
     3661 tttttcctcg tttataaggt gttttttgag gttgctgagg aagtttgtga ggataagcaa
     3721 cgccaattga aataatcgtt ttagccgatt ctaaaagcct ttcagggtag atacgatcct
     3781 ctatgacttt atgttcaaat cctgaattgc gcccctcttc cacagaagca cgcaatgact
     3841 tttccaaata atcaaagtta tctgccgtcg taaaaccaat tttagagata ccaatttctt
     3901 ttgctaattt ttgaatttct gcctttatgt ccataatatt attttaagtc aatttaataa
     3961 aagagacaag gtaagaaact gaaaaattaa atcctacata tcaattagta aattacttaa
     4021 acactagtca tcatattcct gaaaatgacg ataaaggtga tctaagtctt taatctcttt
     4081 ggttaacttt tgtccaatag ttgtatcttt tacaagaatc ctatccttgg cttcttctac
     4141 taatcgcttc gtatcaatga tatagttggt tccacttaaa acttcctcaa tggatgtaaa
     4201 aggaaggtgt gaaattaatt gtatcccata gctattataa atcaaagtgt agccagctaa
     4261 acctgtattt ttctgataac ctttggcaaa gccaccatca attactaaca tcttaccatt
     4321 tgcttttatc ggttgctcac catttttttc tttgactggc gtatgaccat tgacaatatg
     4381 tccaaactta ggcaaatcaa actcttgtaa aagggcagtg caaatatcat ctttatctct
     4441 cagtttaaag tagggatttt ttctttcttg atgagttact ttgtcggaaa cataatagcg
     4501 ctcaaaagta gtcatggcat tcttaccaaa aagagaagaa ttttcaccac accaaagata
     4561 ccaaacgata tctgtcgcca aatcatcatg tttctctggt ctagcatatg ctagacgtat
     4621 ttgactttca aataaatcca gtaaatccct accaccataa gtttttcctg caattttaaa
     4681 gcttttaaaa tcaccattac tatgcattgg tatgcaacca tggaataaaa gattatcatt
     4741 ataggttaaa tacatacttc ctttctcaaa taggaaatca atatgtttct ttaaactttc
     4801 agaagtctgg aaggctatca tcaattgttg taaaagctct tcttcctcac ttgttaatcg
     4861 actgggattt ttcgaatcaa tgcatctact attaaaatct ttaagttgat gcacaactcc
     4921 ctttatagat atacttaaat ctttataatt tacctggtta ataagttgtc gattatgcat
     4981 ttgaaattct ggacgcctgt caatcaattg ggcttccaat ttaaactgta aaattgctgt
     5041 tgcttgttgt atcatattta acaaatctaa ttcatctgga tgagtcattt cctctccatc
     5101 taatatgggg gcgaaagaag gtagaggttc atagtaacgg cgtgagtaat caaccaagcg
     5161 acgcaaattg ataccatatc ggtcttcaat taaagtaata ttattataac gtgcagcaat
     5221 acggataaca tttaccatac aaaggtagga accagatgct gctcccatcc aagtgacatc
     5281 atggttcccc cattggatat ccaaatttgg aaaagccatt aacctattca agatacgatc
     5341 aggataccta ccacgatcgt aaatatcccc tacaacatgt aaatggtcaa tgattaaaac
     5401 ttgaattgtt tgggataata ctataattaa atcttctact tggtccaatt caaataattt
     5461 ttcaataata gtatcaaaat agcttttctt ctctgggtta cggtcaatct cagctaaaag
     5521 ttcttcgata atgtaagcaa acttacctgg caattgctta cgtactttag attttgtata
     5581 tttactagaa aaatatttta aaacttgtat ttgtaacgga atcatttccc ataacttcgt
     5641 tttataagca cttactgaca agttcatttt atcgaaggct aatttttcct ttgggtaata
     5701 aagcagtata caaaaatcat ctaaatcaac agcaactatt ttctgccaat caaagcagtc
     5761 taataattta gctctaatag accctgcacc tgtccgtaag agataatcaa cagcatcgta
     5821 ttccccgtgt aaatcactaa taaaatattc cgtccctttg ggtaattgac aaatagcttc
     5881 taaattgatc atttctgtta cgatatcttc ttttcgagga aatttttctt tcaagagctt
     5941 ataaaaatta gacatgagac ctccttaagg caccttaaat ataaagcttc taaacacaat
     6001 tatgaaagcg cattctacaa tgacatatct attatagaat gactttaaaa ctatttcaaa
     6061 aatttactgt ttatctcttt gaactacaat atggtacaat acccttatga ccaaacttgc
     6121 tatcatgagt gatttacata ttgacctcaa tcatttttct acctttgaga cgaaaacgtt
     6181 aattaatttg cttaagaaag aaaaaattga tcacctccat atcgctgggg atatatctaa
     6241 tcattttact aaagatacac ttccctttat caataactta aaaaagcata ttaaacttag
     6301 ctataattta ggaaaccatg atatgcttga tttaacagaa acagaaatcc aacgccttga
     6361 ctttcaaacc tatcgatttg acaaaaaaat gctactagct tttcatggct agtatgatta
     6421 cagcttttca aataatagag atatcaagga tgttgaaaaa ttaaaaaaga ctttttggtt
     6481 tgatagacgt ttaaaacgtc caaataatga tgtaactatc caagcatcaa ttttaaaacg
     6541 cttagacgaa atattagcaa aagtcgattc ttcaaatatc attattgcta tgcactttgt
     6601 tccccataaa cagttcacga tgactcatcc tagattctct ccttttaatg cttttttggg
     6661 gagtcaggct tatcatgatt tattccaaaa atatcatatc aaagatgttg tttttggtca
     6721 tgcacaccgc tcatttggcg acgttaaaat tggagagact acttatcatt ctagaccact
     6781 tggatatatt agagaatgga atttaactat tgactttgtc aatcagaatc ccaatcataa
     6841 ccctaattta acctggaatc ttagcaaaag gcataatgcc gttaagcatt tggatagttt
     6901 tgaaaactat ccaaaaaaat attttgaaga cgaattacgc aactctatga caatatttga
     6961 ttgctaaaaa actcacttag atattctaag tgagtttttt tatcagctaa actaaatagc
     7021 tttttcgtct tgtcctaatt tacgttggat aaatttaaca atttccgtta acactaccat
     7081 caataaactt ccgataacta cagttaacca ttgtgttgaa cttaaatgag ttacatggaa
     7141 aagtttatta aatccaggaa caacaatagt caccatcaaa agaatgaaag ctactggaat
     7201 agaccagtta aaggttctat ttttaaaagc tccaactgta aagattgatt ggtaaactga
     7261 tttaacgtta aatgcatgta ccaattggat taaaccaagc gttgcaaagg ccatcgtaag
     7321 ggcatctgcg tgaatcatgc gataaccagc atgttctgga tacatcaatg cccaaccata
     7381 gacaccaagt actaagatag tttgtaaaat accttgatag ataatagctc ccataacacc
     7441 accatcaaag aagtttgact ggcgaccacg aggtttatgt gtcataactc ctggttcagc
     7501 tggctcaact ccaagagcga tagctggaag agtatctgtt accaagttaa tccaaagaag
     7561 gtgaaccggt gcaagtacat cccatcctaa taaagttgca aagaaaattg taaatacctc
     7621 tgccatgttg gctgaaagaa gatattgaat agatttttgg atatttgaga agactttacg
     7681 tccttcctca acagccacaa taatagttgc aaaattatca tcagcaagaa ccatatcaga
     7741 agcacctttg gaaacttctg taccagttat tcccatacca ataccaatat ctgctgtttt
     7801 tagcgatgga gcatcattaa caccatcacc agtcatggca acgactttgc catcgttttg
     7861 ccaagctttc acgatacgca ctttatgttc aggagaaaca cgtgcataga cagaatattg
     7921 tttaaatact ttttgaaact cttcgtcact taattcatta agttcagcac ccgtaaagac
     7981 atgatcttct gtgtcatttg catcaataat acctagacgc tttgcaatag cttctgcagt
     8041 atcttgatgg tccccggtaa tcataatcgg acgaattcct gcttctttag caactcgaac
     8101 agcttctgcc gcttctggac gctcagggtc aatcattccc actaaacctg agaatactaa
     8161 atctgactca acaatatcag tctctaaagt tggaatttgt gtttcatatt tataagccat
     8221 catcaagaca cgaagagctt gttttgcgag ttctttattg gtatttagga tagcctcttt
     8281 atcttcagcc gtgatatcac gaaccaagcc attatcttca attttagtaa ctcgttttaa
     8341 taattgatca ggagcaccct tgactgcgat aaagtagcga ccatctgatt ctttatgaat
     8401 cgtagacatt aacttacgat ctgaatcaaa tggtaactca gcgacacgtg gttcattttt
     8461 caaaacttca cggacatcaa aattcttatc taaaccaaat tgtaccagtg cagtctctgt
     8521 tgggtcaccg attaattttc ctgaaggatc aatcttagta tcgttggaga aattcataat
     8581 acgtaaagtg ttattatcaa ctgaaatttc ttccgaagat gattggagaa cgccattagt
     8641 atacactttt tcgacagtca tttgattcat tgtcagagta ccagtcttat ccgatgcaat
     8701 gatttctgta gaacctaaag tttcaactgc agggagttta cgaacgatag aattacgttt
     8761 agcaagagtt tttgttccca ttgataagac aatcgtaaca attgcaggaa gcccttctgg
     8821 aatagctgca actgctaaag caactgaagt catcaatcct tcaatccaac cttctttacg
     8881 gacaaatatc cccactaaga aagtaataac cgcaataatc acgattaagt aagtcaataa
     8941 tttagaaagt tgaactaaac tttgtttcaa tggtgtttca gattcatcag catttgccaa
     9001 catatcagca attttaccaa cttcagtata catccctgtg ttagtaacaa caccatatcc
     9061 tcgaccatac gtgacatttg aattttgata cgccatattc acacgatcac caatacctgc
     9121 atcctctgct actacttgag atatgtcttt ctcaacaggt actgactcac cagttagagc
     9181 tgcttcttca atttttaaag acgcggcttc taaaagtcgt aaatctgcgg gaacaacatc
     9241 tcctgcttct aacataacga ggtcaccagg aactagttct ttagaatcca cctcaatagt
     9301 atgaccatca cgacgaacac gcgcaattgg gcttgacata tctttgaggg cttcaatagc
     9361 tgcttcggct tgcccctctt gataaacacc aaaagctgca tttaagataa caactgccaa
     9421 gataatcaat gcatccgtta agccatgcat accttccgta ataacagaga gagctgcagc
     9481 aaccagtaag ataataatca ttaaatcttt gaattggtca aaaaacttag ctattagact
     9541 acgttttttc ccttcttcaa gctcattgcg accatacatc tctaagcgat ttttcgcttc
     9601 tgttgttgat aatccttcac gagaagattc taaagacgtt agaacctctt cttgaccttg
     9661 tgtgtaaaac aaggattttt tctgttcttt taacaaaacc tttcctcctt tggtctctcc
     9721 acaaacaaaa agagacctgt ttttatgaaa acaagtctcg ctgtttaaga caacgccgga
     9781 aatttctttc gtaatgacga cattgccacg gtaaaccgtc tgctactccc ttgatatgtt
     9841 aatcattgta acagaaaaaa atccattttt caattaatta tccttgctat cactaataat
     9901 ctcaagggaa aaaacgaagt tcatctagtc gaaataaacc atactgcagt ttatcaaata
     9961 aatggctctc ataaagagat cccagcgtct tcagtagacg aaattcataa tgactactct
    10021 tttgttaatg gtaattatct caataccatt gaatctttaa ttcataattt gcaaaacagg
    10081 ctcctgtctc cgtttgctta aggacataat tgatgtgcaa cctttgctgt gacaaatcaa
    10141 actgatagtg acttgtctct gtaatcaaat gttgaatacc aagtggagtg gggatattaa
    10201 ccaaagcagg cgtttcacga tgaaaacgca ttacagactt aggatttgaa aacctcgtca
    10261 taagcaattc ctcatgattt gccttaataa ctactctctc gccctcagca ttaatataat
    10321 tcaaataaac gtaacttcct ttttcaataa attgacaatc gtgaatctca tgaatcattt
    10381 cggtttcgtt gtcaatttga atgttattgt taatttctag tttcatgact tctctcactt
    10441 cttttatcct tctattctac caatttctga ctcaagtttc aatttttgct ataatatata
    10501 tatgaatgaa aaagtatttc gtgaccccgt tcacacttat atccatgtaa ataatcaagt
    10561 catatatgac ttgattaaca ctaaagaatt tcaacgttta cgacgcatta aacaaacctc
    10621 aacgacttct tttaccttcc atggtgcaga gcatagtcgt ttttcacatt gtctcggagt
    10681 ttatgaactg gctcgtaaag ttacagaaat ctttgatgaa cattattctg atctttggaa
    10741 taaaaatgag tccctgctaa cgatggctgc cgccctacta catgatatcg gacatggcgc
    10801 atattctcat acatttgagc gtctcttcaa tactgatcat gaagcttaca ctcaggaaat
    10861 catcaccaat cctaccacag agattaatgc tattttacga aaggtggctc ctgattttcc
    10921 tgataaagta gctagtgtta ttaatcatag ctaccccaat aaacaagttg ttcaattaat
    10981 ttctagtcaa attgattgtg atcgaatgga ctatcttctc agagattctt actacactgc
    11041 tgctagctat ggacaatttg atttaacacg aattttacgc gttattagac caaccgattc
    11101 tggaattgct tttgctcgta acgggatgca cgctgtggaa gattatattg ttagtcgctt
    11161 ccaaatgtat atgcaagttt atttccatcc agccagtcgt gctatggaat tactcttaca
    11221 aaatttacta aaacgtgctc gcttcttatt tgatacccat cgcgattttt ttgaacaaac
    11281 ttctccaaat cttattcctt tcttcacgga tcaatatgat ttacaagatt atttagccct
    11341 agatgatggg gtaatgaata cttacttcca atcttggatg caagctgatg ataacatttt
    11401 ggcagattta gctaatcgtt ttatcaatcg aaaagtcttt aaatcaatta cctttgaaga
    11461 ggctgataaa gaaaatctag ttaaaatgaa agaactagtc tcacaggttg gttttgatcc
    11521 cgattattac actggtgtcc atgctaattt tgacttacct tacgatgttt atcggcctga
    11581 acattcaaat ccacgaacag aaattcaaat tatccaaaag aatggacaac ttgctgaatt
    11641 atcaagctta tcaccgattg taaaagcatt aactggatct aattatggtg atcaacgttt
    11701 ttattttcca aaagaaatgt tgacattaga tagcctattt tcaagtacaa aagaagaatt
    11761 tcaatcttat atcacaaatg agcatttgac actcacgaaa gataatagcc attcatgaca
    11821 aaccctttat tttttagtaa gatataaggt gagtatttta ggatattgaa ataaagagtg
    11881 ggagataaaa tgacttcaat taaacttgtt gccgtagata ttgacggcac cctattaaat
    11941 agtaagcgtg aaattacacc tgaagtggct aaagctgttc aagaagcaaa atcaaaagac
    12001 gttaaaattg ttattgctac aggacgtcca atcatcggag tccaagatct tttagaagaa
    12061 ttgaaactca atgaagaggg agattatgtt atcaccttta acggcggtct agttcaagac
    12121 acagccacag gagatgacat catcaaagaa accttaacct atgaagacta tctggatttc
    12181 gaactgttag cgcgtaaatt aggcgtccac atgcacgcca ttacaaaaga aggaatttac
    12241 acagcgaatc gtgatattgg taaatataca atacacgaag caactttagt taatatgccg
    12301 ctcttctatc gtactcctga agaaatggga gacaaagaaa tcattaaact aatgatgatt
    12361 gatcaacctg acatcttaga tgcagcaatt gctaaaattc caaaaaaagt tctcgataac
    12421 tacaccattg taaaatcaac tcctttttat ttagaaattt tacccaaaaa cgttaataaa
    12481 ggcactgcac ttctccacct tgcagagaaa atgggattaa ctgtagatca aacaatggct
    12541 attggtgatg aagaaaatga ccgtgctatg cttgaagttg ttggaaatcc tgttgttatg
    12601 caaaacggca atcctgaact aaaaaaaata gcaaaatata tcaccaaatc aaacgaggaa
    12661 agtggcgtag cctacgcact acgtgaatgg gtaataaact gatgtatcat gttaccgttg
    12721 gaatatccga aaaagaatat gatgcctttg ctattgcatc gtctcaaacc aatctcctcc
    12781 actcttcaaa gtgggcacaa gtaaaatcaa attggcaaaa tgaacgttta ggcttttata
    12841 aagatgacca attagttgct gtagctagca tcttaattaa atcattacct cttggtttta
    12901 caatgcttta tattccacgt ggacctatta tagactacag caataaagaa ttagttggct
    12961 ttgtgttaaa aacactaaaa aactttggac gaaagaaacg tgctgttttt gcaaaattcg
    13021 atccagcact tcttctacgt caatatcacc ttaaagaaga gaatgtcgcc gaagagatag
    13081 atgaaagtag acaggcaatt gataatctta agtcagcagg tgcccaatgg ataggtccaa
    13141 cgaaagccat ttccgaaacc atccaaccac gttttcaagc gaacatctat accaaagcaa
    13201 atatagaaga aaactttcca aaacacacta aacgtctgat aaaagatgca aagcatcgcg
    13261 gtgttcaaat ttatcgtgcc aacattgatg atttacctaa attcgccacg gttgttaacc
    13321 gagaatcgta aaggtgtagc tttacgtaat gaaaactatt tccatcaact aatgacaatt
    13381 tatggagaag atgcctatct ctatttggct aaagtcaatc ttcctaaaag attagcgcaa
    13441 tttaaagaac aacttcttca aatccaaaga gatttatcag aaactccatc ccatcaaaaa
    13501 agtcgcctga caaggctaaa tcaacaagaa gcttctgtta aacaatatat ccttgaattt
    13561 caggagttta gcaagaaata tccagatgag cctgtcattg caggaatttt gtccattcgt
    13621 tttggcaatg tcctggagat gttgtatgct ggtatggatg atagcttccg taaattttat
    13681 ccacagtatc tcttgaatgc tagggtgttc gaagatgctt tcaaaaacgg tattgtatct
    13741 gctaatctag gcggtgtcga gggaagtctt aacgatggtt taacaaaatt taaaagtaac
    13801 ttcaatccaa tgtttgaaga atatattggc gagtttaatc tagcaatcga tccattatta
    13861 tataaattgg ccaacctagc ctatactatt cgaaaaaaac aaagacactc tcactaagaa
    13921 aggaaactca tgtatagaga aattaccgct gtcgaacacg atcgctttgt gagcgaatcc
    13981 aaccaaacaa acctacttca atcttctaat tggcccaaag taaaagacaa ctggggtagt
    14041 caattacttg gcttttttga cggtgaaacc caaattgcca gcgctagtat tctcatcaaa
    14101 tcacttcctc ttggcttctc catgctgtat attccgcgtg gaccaatcat ggattactcc
    14161 aatctagata ttgtaactaa ggtccttaag gaccttaaag cttttggcaa aaaacaaaga
    14221 gctctcttta tcaagtgtga tcctctcatc tatttaaaaa tggtcaatgc caaagatttt
    14281 gaaaattcgc ccgatgaaaa agaagggtta atcgccattg atcatttaca gcgtgcaggt
    14341 gctgattgga ctggtcggac aacagattta gctcatacta tccaaccacg ttttcaagca
    14401 aacctatatg ctaatcaatt tggacttgat aaaatgtcta aaaaaactcg tcaagctatt
    14461 cgaacttcta aaaataaggg agtagatata caatttggta gccatgaact acttgaagat
    14521 tttgcagagt taatgaaaaa aacagaagac cgtaaaggga taaatcttag aggaattgac
    14581 tactaccaaa aactccttga tacgtatcct aataactcat acatcacaat ggcttcgcta
    14641 gatgttgcaa aacgattaga aaaaatagaa aaagaatgcc agatagctca atctgaaaga
    14701 ataaaatcac ttgaacttaa tcgtgagaaa aaagttaaac aacaccaaga tacgattgat
    14761 cgattaaata aagaaattga ttttctcaaa gaagctcaaa aagcctatga ccgagacatt
    14821 gttccattgg ctgcaaccct cactctagaa tttggtaata catcagaaaa tatttatgct
    14881 ggtatggacg attacttcaa atcttattct gctcctattt acacttggtt tgaaacagct
    14941 caaagggctt ttgaacgtgg aaatatttgg caaaatatgg gggggattga gaatgactta
    15001 tctggtggtt tatatcattt taagtctaag ttcgaaccaa tcattgagga atttattgga
    15061 gaatttaaca ttccagtgaa cagattgctt tataaagcct ctaattatgt ctatgcctta
    15121 cgcaaaaaac gtaatagcta gttaggaaag gatactttta tggcgcttaa agaattaaca
    15181 gctaaagagt tcgaaagtta ttcaggaaat tacgatttac aatcgtttat gcaaacccct
    15241 gagatggcaa agcttctaaa aaaacgtggg tatgacatta cctacatggg ctaccaaatc
    15301 gatgggaaaa tggaaatcat cagtattgtt tataccatcc caatgactgg tggacttcat
    15361 atggaagtca attctggtcc agcccattcc aattctaaat atctgaaaca tttttataaa
    15421 gaactacaga actatgccaa atctcagggc gctttagaat tattaattaa accttatgat
    15481 acctaccaag agtttactgg tgaaggtgaa cctaaaggtg caccgaacac ctatcttatt
    15541 gatggcctga ctagtatagg ttatcaccac gatgggcttc atattggtta tcctggcggt
    15601 gaacctgatt ggcattatgt taaaaatcta gaaggtatca ctcctcaaaa tcttcttaaa
    15661 tctttctcta aaaaaggacg ccccttggtt aagaaagcca tgtcttttgg tataaaaata
    15721 cgtgtcctca agcgggaaga actacatatc tttaaggata ttacttcttc tacttctgat
    15781 cgccgggact atatggataa atctctagat tattatcaag atttttatga tagtttcgga
    15841 gataaggcgg aatttgtcat tgcaactcta aactttaggg aatatgatca taacctacaa
    15901 ttaaatgcaa agaaattgga agaacagatt acagtccttg ataatcgcca tcaaaataat
    15961 acagattctg ccaaatacca tcgtcaacgt actgagcttg tcaatcaact agctagttta
    16021 gataaacgtc gcaaagaagt cgaacctttt attcaaaaat ttggaaatca agatgttgtt
    16081 ttagctggta gcctctttat ttatagccct aaagaaactg tctatctttt ctctggctca
    16141 tacacagaat ttaataaatt ttatgcgcct gcagttcttc aagaatatgt tatgcaagag
    16201 gctctaaagc gtcaatcaac tttctataat tttttaggta ttcaaggaaa ttttgatggt
    16261 tcagatggcg tacttcgctt taaacagaat tttaatggtt acattgttcg taaaatgggt
    16321 actttccgct actatccaaa tccattaaaa tataagagta ttcaattact aaagaaaatc
    16381 ttacgccgaa cataaaaaat aagcttacat aagcttattt ttttatgcgt tatagaaagc
    16441 aacatttatt tctttactat tattgtttac acaaataa
//
LOCUS       DATRME010000044        15455 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRME010000044 DATRME010000000
VERSION     DATRME010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15455
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(41..2836)
                     /gene="ppc"
                     /locus_tag="VBC23_001358"
     CDS             complement(41..2836)
                     /gene="ppc"
                     /locus_tag="VBC23_001358"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6843302.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            3045..4844
                     /gene="pepF"
                     /locus_tag="VBC23_001359"
     CDS             3045..4844
                     /gene="pepF"
                     /locus_tag="VBC23_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6843303.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(4903..5292)
                     /locus_tag="VBC23_001360"
     CDS             complement(4903..5292)
                     /locus_tag="VBC23_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6843304.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(5276..5800)
                     /locus_tag="VBC23_001361"
     CDS             complement(5276..5800)
                     /locus_tag="VBC23_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6843305.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            5957..6805
                     /locus_tag="VBC23_001362"
     CDS             5957..6805
                     /locus_tag="VBC23_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6843306.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(6838..7455)
                     /locus_tag="VBC23_001363"
     CDS             complement(6838..7455)
                     /locus_tag="VBC23_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO6843307.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
     gene            7749..8222
                     /locus_tag="VBC23_001364"
     CDS             7749..8222
                     /locus_tag="VBC23_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6843308.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            8244..8885
                     /locus_tag="VBC23_001365"
     CDS             8244..8885
                     /locus_tag="VBC23_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6843309.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(8921..9823)
                     /locus_tag="VBC23_001366"
     CDS             complement(8921..9823)
                     /locus_tag="VBC23_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6843310.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            9998..11488
                     /gene="lysS"
                     /locus_tag="VBC23_001367"
     CDS             9998..11488
                     /gene="lysS"
                     /locus_tag="VBC23_001367"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6843311.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(11563..12033)
                     /locus_tag="VBC23_001368"
     CDS             complement(11563..12033)
                     /locus_tag="VBC23_001368"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6843312.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(12048..13241)
                     /locus_tag="VBC23_001369"
     CDS             complement(12048..13241)
                     /locus_tag="VBC23_001369"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6843313.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(13259..13909)
                     /locus_tag="VBC23_001370"
     CDS             complement(13259..13909)
                     /locus_tag="VBC23_001370"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6843314.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(13890..14999)
                     /gene="ribD"
                     /locus_tag="VBC23_001371"
     CDS             complement(13890..14999)
                     /gene="ribD"
                     /locus_tag="VBC23_001371"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6843315.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(15193..15307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         5017 a         2991 c         2410 g         5037 t
ORIGIN      
        1 agtaaaaggg agaatagcaa tgatcctccc tctatcaata ttaacctgaa tttcgtaaac
       61 ctgttgcaat accattaata gtaatatgaa tcaacttctc atagtcttca tctagttggt
      121 tactacgaag acgtttaatc aattcaattt gaacataatt caaaacattg aaatatggca
      181 aacgataatc taaacttgca tgtaacattg ggttttcttc taataaatta tcatgctgtt
      241 caatagctaa aatcatatct ttagttaact gccattcatt aaggatgata ttaaaaacat
      301 ctcgaacttc tttacttcca gctaactgag catactgaag cgcaatattc atatttgatt
      361 ttgataaaac catatctaca ttagataaaa gagaattaaa gaatggccat ttttgataca
      421 tggtttgtaa ttttgctaag tttgcctcat cttgttcaat aaattgcttg aaggctgacc
      481 caacaccata ccaacccgga aacataatac gattttgtga ccatgaaaat acccatggaa
      541 ttgcacgtaa acctgaaatt tctgtaattg ttttacgtgc tgctggccga gaaccaatat
      601 ttaaacttga tacctcttta attggacttg cttcaaagaa atagtcatag aaataaggat
      661 tatcaaaaac taaatttcta taaacggcat tactttcagc cacaatacca tccattgtct
      721 cacggaaatt atcaatttca ttaaggttag taatcatacg tgttaccatg cggtctatgg
      781 aagctgaaat aagcatttca aggttataat aggctgcatc ttgattacca tatttattct
      841 caatgatttc accttgctct gttaggcgaa ttcgatcttt gattgaacca aaaggttgtg
      901 atgtgatagc ttcataagat ggaccaccac cacgaccaac agttccacca cgaccgtgga
      961 aaaatgtgat tttaataccg ttttcttcac ctatttttgt tagctcattt tgagctttat
     1021 atagagtcca accagatgat aaatagccac catctttatt actatctgaa taacctagca
     1081 ttatttcttg ataattgttg ttagttgtaa tccatttttt tactaatcca taatgtagat
     1141 actgggtcat aatatcacgt gaattatcca aatcttcgat ggtttcaaat aacggtacaa
     1201 tctgaatgcg agcttgattg gcatcaatta gtccaacttc ttttaacatg atagctaact
     1261 caaacatatc cgaaacgctc tctgtgtgag aaatgatatg ctgttttata atgtcttcac
     1321 ccaattgatc ttttaactca cgtgctgttt ggaaaatagc caattctttt tgaagtaact
     1381 cggatttagg agtatgcgta gaagataatg ttctaggatc ttctgttaat tcttttaaga
     1441 gaagttgaca tttttcttct tctgaaagag aactgtaatc atctacaata ttggcagact
     1501 tcaataattc tgctacacaa gcttcattaa tactagaatc ttgacgcata tcaatagtag
     1561 ctaaataaaa tccaaagata tctactgctt gaataagaca agctaattcg ccatcgacaa
     1621 gagaatcttg tccatgttga actaaagatt gcttcacaag taacaaatct tgtttaaact
     1681 cctcagcagt agcgtatgct ggtagttctt ctgtctgtat gtcagaagct acttgagcga
     1741 tatgtgattt tatatacctt cctacaatac cagaagattc ttggcgacta acaaatttct
     1801 cttttgggta agaaccaact tttaaattta gtagggtttg tactagttta tcttggataa
     1861 aattgaaagc tttacgataa ggttcatttt ccctatatac tgaattatct tgtgactggt
     1921 ttgccaacgt aacaagttcg ggagagactt cagttagact tattgaaagt gacatattac
     1981 gataaagttc atttagctgc tcaatataat ggttaataat cacttcactt tgtaccatag
     2041 ctgataatct tagtgtctca gcagttacaa agggattgcc atctctatca ccaccaatcc
     2101 acatccccat agtcagaggc ttaggattct ctaagtgaat acctttttta gctgccagtg
     2161 ccttatattc tgctgttaat ttggtaactg ctttaataag agaacggttg taatattcca
     2221 ttacattggt tatctcattt ttgactttca attttttctc gcgaatagtg tctgtttgca
     2281 tgataatgcc gatgtaacga cgcaagtcag cataccattt ttcttgattc acgattcctg
     2341 ctttgacatc tctgtattta cgtaataaat catgaatctt agacgttaat tcgagcaccg
     2401 ttttacgctg cacctgagta ggatgagctg ttagcacagg cacaacattg acgtgttcaa
     2461 gaatatcttt agcattttca tggccagcta caacatcaat agtcgttgat aactttccta
     2521 ggtaatcttg gttaagatta tttttataat taatttcata tgctaaatca acgtcttctg
     2581 aaatattaat taagagcggc aaaatagaga agtaacgaga aacaataacc atctcctcgt
     2641 tgcttaaagc cgaaatagtt tccttcaaac cagtataatc gtcagttgat gataatgaaa
     2701 ctattttttc aatcttgtca aatgattcac taccaatcaa tttttgcgtt gcttcatcca
     2761 aaagctgctt cagtaaaccg acctcttcgg taatgatttc tttattgcta ctactttcta
     2821 atttcgggtg ggacaaacca tcacttcctc tcttaaatct cactactatg ataccatttt
     2881 aaagtaaaaa gtaaaccatt ctaggatttt tagatagaat tttcagattt ttacttattt
     2941 atgacagtaa tcagtttttc ttgttcgact tttttgatat actgaggata attgagatta
     3001 actgattaat tgttaattaa caactattat taaaggagta tcgcatggaa cttaaaaaac
     3061 gctctgaatt ccctgaaaat gagctttggg atctgactgc tctctacaaa gatcgtcaag
     3121 attttttact ggctattgaa aaagctcttg aggatatcaa ggttttcaaa aaaaattacg
     3181 agggaaaact taattgtgtt gaggatttta ctagtgccct tatggaaatc gagcatattt
     3241 atatccagat gagtcacatt gatacctatg cttttatgcc tcaaactact gactttagca
     3301 acgaagagtt tgcacaaatt tctcaagcag gatcagactt cgcaactaaa gctaatgtac
     3361 tactttcttt ctttaatact gccctagcaa atgcagatat aaagatttta gattctttag
     3421 aaaataaccc tcatttcaaa gcaactattc gccaagctaa aattcaaaag caacacttat
     3481 taagtccaga agtcgaaaaa gctttgacaa acttaaatga agtcctaaat acaccatatg
     3541 atatttatac taaaatgcgt gctggcgatt ttgatatgga agattttgaa gttgacggaa
     3601 aaacctacaa aaatagtttt gtaacttatg aaaattactt tcaaaatcac gaaaatgcag
     3661 agatacgtga aaaatccttc cgttcattct cgaaaggtct acgtaagcac caaaatgctg
     3721 cagctgcggc ttatcttgca aaagtcaaat ctgaaaagtt aattgctgat atgcgtggtt
     3781 acgatagtgt ttttgattat cttctttccg aacaagaagt tgaccgttca atgtttgaca
     3841 gacaaattga tcttatcatg gatgaatttg gtccagtagc tcaacgattc ctcaaacata
     3901 tcgccgatgt taatggtatt gaaaagatga cttttgcaga ttggaaatta gacattgata
     3961 acgaacttaa tcccgaagta agtatcaatg atgcttatga cttagtaatg aaatcagttg
     4021 ctcctttagg aaaagaatat tcccaagagg ttgagcgcta tcaaaaagag cgttgggttg
     4081 attttgcagc taacgctaat aaagattctg gaggatatgc tgcggatcct tacaaagttc
     4141 atccctatgt actcatgagc tggacggggc gtatgagcga tgtctacaca cttattcatg
     4201 agattggaca ttctggtcaa tttatcttct cagataatca ccaaagtttt ttcaatactc
     4261 acatgtccac ttactatgtt gaagctccat ctacttttaa cgaactttta cttagtgact
     4321 acttagaaaa ccaatttgat acagcacgtc aaaaacgctt tgctcttgca catcgcttaa
     4381 ctgatactta cttccacaat ttcattactc acttacttga agctgctttc caacgtaaag
     4441 tttacactct aattgaggag ggagaaactt ttggagcaga acaattaaat gctattatga
     4501 aggaagtatt aacacaattc tggggagatg ctattgaaat agatgacgat gctgctttga
     4561 catggatgag acaagctcat tactacatgg ggctctatag ctatacttac tctgctggtt
     4621 tagtaatctc gacagccggc tatctcaatc tcaaaaataa tccaaacgga gcaaaagagt
     4681 ggttagcatt cttgaaatct ggtgggagct gtaccccttt agaaacagcc ctattaatcg
     4741 gtgctgatat ttctactgat aagcctctac gcgatactat taatttctta tctaacactg
     4801 tagatcaaat tattaattat agtaatctta ttaaacaagg ctaaactaca gtctttcaaa
     4861 aacataaaaa caagttggag atatctccaa cttgttttta tcttattttt tgtaaacttc
     4921 ctgataaaat tcaattttgt cgttcttttt aactttaatt tggtttgctg ccttaggagc
     4981 taatttatta ttgaccttga acatccaata aagccctttg gtttcatctt ggctgatgcc
     5041 atcaattgaa gtaatgaaac catctttttc tttaacttta tagttagctt taagcacatc
     5101 caaaacagtg tcacctttat ggtaaacaac cttttcactt aacacgttcg tatcttcttt
     5161 aacaaccaat tcaaccttac caacttctaa cgttgagcta gttgttgtct tattcgatgt
     5221 agatggttgc ccgcaagcta ctaaagtcgt agcgaatact gttagcaaga ataatttaat
     5281 gtattttttc attcctaaat ctccttaaaa tcggtaacaa aagaggataa aataaacaag
     5341 ttgataaagc gtgtgccata ttaaaactaa gtcctgctaa tacataagtc caccaaggca
     5401 tatgatataa ccaagcaaaa caggtatcaa taatgacgcc ataaacaacc cctaaccctc
     5461 ctgcaaagaa agtttgaagt actacctccg ttattttacc aaaacataca gcttttgtca
     5521 gcggatatat cagaaatttc cacaagcata aaattaaagt gaaggaaatg atttgcaaaa
     5581 agacccatgg cccaaagccc attaaaaaag aagaaactag cattgttaca gttacagtag
     5641 ctacaccttc ttttaaatcg aataagacaa ctgtaatcaa aaaaatggca gtaattggct
     5701 gaatgttagg cagaggcgca aaagcatatc tcaacacaac acacaaagca gataatatag
     5761 ctactctagt caatttagtt aaagttgaat tctttatcat gtctttaatt atacctcaaa
     5821 actatgacta ccgcttaaaa gtctttttta ttacaatttc atttctaatg ttacataaac
     5881 gacctctttt tttaaaagaa cgtaaataag cctgtaaact ttgatttttt aaacctttta
     5941 agttacaata tcaataatga gaagaagaat taaacctatt gttgttgcgg tttttttcag
     6001 tttatttgga cttttattaa ttattggtca tttacattcg acaaatactt tgaaaaaaga
     6061 attagttgaa gctaaaaaga caattccatc cgtaaaagct tcaaaagtac cgcaaaaatc
     6121 aacatcatcg aaagataaag agtttgttct taaaccgatt atcgatgtct ctggttggca
     6181 acttcctaag gagattgatt acgatacgct ttcaaaaaat atttcaggtg ttgttattcg
     6241 tatctttggt ggatcaaaga tatctaagac taataacgct gcttatacaa ctggaatcga
     6301 taaatcgttt aagacccata tcaaagaatt tcaaaagcga aatatcccag tagctgtcta
     6361 cagttatgca cttggttcaa gtgttaaaga aatgaaagaa gaggctcaga tattttataa
     6421 gaatgcagct ccttacaaac caacttttta ttggattgac gtagaagagg agacaatgtc
     6481 taacatgaat aaaggtgtcc aagcattccg aaaagagtta aaaagacttg gtgctaaaaa
     6541 tgttggtatc tacatcggta cttactttat gactgagcaa ggtatctctg taaaaggatt
     6601 tgacgctgtt tggattccaa cttatggtag cgattctgga tactatgaag cagctccaca
     6661 aactgaactt aaatacgatt tacaccaata cacctctcaa ggttatctac caggattcaa
     6721 tcaaccgctt gatttaaatc aaattgcagt taataaagac aagaagaaaa cttatgagaa
     6781 actttttgga aaagtaaaag attaaaagct actgttaaca gtagcttttt tgataaacta
     6841 tttattattt aattcttcaa tagaaaggat tttttgagtt ggttttttcc taagcgaagt
     6901 aacgattgct tctgccacta tcacaacctt agttggaaag actttttgta caacaatacc
     6961 taagaaaggc aaatgactaa ataattttat acacttggct tggaaaatcg agagaggtcg
     7021 ctcttcacca tacatcaaac ctggtcttac aaaaagataa tccagaccgc ttgctttgat
     7081 tatctgctct gccttccttt tacttttaat gtaagctgaa tagccgctat tggctgaaat
     7141 gtaaactaac tttggtattt gatttttgtg acagagtgct actgcttttt gggttgcttt
     7201 aacgttaagc tcatctagtt gattgggctt aatcgctcca atacagtcaa ttaatatatc
     7261 aaaatttcta tgttctaaat gaatcttatc agcttctgta atatctccct taatgtaggt
     7321 taatctagga tccttaaata tatcaccttt accttcatgc cttgataagt aagccacctt
     7381 atgccctttt gtaagcgctg cttttattat ctgcttccct agaaaaccac taccacctgc
     7441 aatcagtatt tccatagttc cctctccttc attttgtaaa tttagtatag aaatttttat
     7501 atgccttgtc aaagtaatcg tttaatacct gaccagaata tgatttcaag tcattacatt
     7561 aggacacttc atatttagct atttataaaa cttctaattt aacattccaa atatactgtt
     7621 gcgatattta ttatcattat tccctgaaat ggtattcaag cttatttcta agactcagta
     7681 gaaatttact ataatcatct ttaaatgtta taataaatat gaaacaaact tattagttaa
     7741 gggtcattat gagtaaaaag aataaaatca aaaaaacttt agttgatcaa attttagata
     7801 aagcaaaaat cgaacatgat agtctacaat tggatgcttt acagggagat ttacccaacg
     7861 gtattcaaaa acaagatatc tttaagacat tagcgctaat cggtgataaa actggtccca
     7921 taataggtat cttacctctc accgagcatt tatccgaaaa aaagttagct aaaatttcag
     7981 gaaacaaaaa ggtacaaatg atacctcaaa aagatctcca gaaaataaca ggttatattc
     8041 acggagcaaa taatccaatt ggaattcgcc aaaaacataa ttaccctatt tttatcgata
     8101 ctatcgcttt agaaaaacaa gaactcattg tctctgcagg agaaattgga cgttctattc
     8161 gtattaatag tgaggttctt gcagatttcg taaatgctaa atttgccgat ataaaggaat
     8221 aataattagg attggagaaa aatatgaaat tttatcttgt tagacacgga aaaacacaat
     8281 ggaatttaga agggcgtttc caaggtgcta acggagactc acctctcctt gaagaagcta
     8341 ttgaagaatt agaagaactt ggacaatacc tatcatctat tcattttgat gctgtttatt
     8401 ctagcgactt aggtcgtgct agagatactg ttaatatcct caatgatgct aattcatgcc
     8461 caaaagaaat ccactatact tctcagcttc gtgaatgggc attgggaact ttagagggtt
     8521 gtaaaattgc aactatgcaa gctatctatc ctcgtcaaat gaccgcattt tatcaaaatc
     8581 ccttacaatt taaacatgac atgttcggtg cagaatctct ttatcagaca acgcatcgtg
     8641 tagagagttt cttacgttct ttagcttcaa aaaattacga caaggtcctt attgtaggac
     8701 atggagcaaa tctaactgcg tctattcgtt ccttactggg ttatcaatat ggtagtttac
     8761 actacaaaga taaacttgat aatgccagcc tcacaatcat agaaactcat gattttaaag
     8821 acttcaactg tctgacttgg aacgataaat cttatttaag gcaagaggtc aaaatgacac
     8881 actaaaataa tccactagat tcatctagtg gattatttaa ttatctagca tacttcatgc
     8941 gtaatacttt ttcctgtttt gcagttaggc gcagtaaaat aacaactttg tcaacgctca
     9001 tattactctc aagcagacgc tctgctgcca tcattaaacc attattaagt cccatttcaa
     9061 ggactggatt acttttatcg ttgatatcaa aaataaattt attatcaggt aagtcaaaca
     9121 acactttaac agcaccgaat aattgctcaa agttatcaat agcaacatca taaactggtt
     9181 taattcccgg ttttaaagaa cgtcctctat ggttgaacca catagaatgc caaccaccat
     9241 taaatgcacc cataatatca ttatcatatg aatcacccac ataaagtgta gttgaagggt
     9301 tcatatcaaa ttgctctgct gctaaattaa aaatctcctt ttcaggcttt tgaaaaccag
     9361 ttgcttgact aactataaca cgttttggat caacatagtc ataaagtccc aattttttaa
     9421 cttttttcaa ttgatgttcc gtagggccat ttgtaataat tcccattggt acattttttg
     9481 atttcaaaaa gtcaagtgtc atacgcatct catcaagcat tgtaatattt tcaagttcat
     9541 gttcgtatat ttcttgaaaa taaataccag tagcttcatc aatttcacga taaccaaact
     9601 ctaaaagcgt ttctttgcat cgccagaaac gaaaatattc tgttgtccat tcaccagcca
     9661 ttactcgtgg aaagccaata tctgaataat gacgaaagcg aatataagct tggttaatag
     9721 cgctcatatc aaaatcagga aagcatttct ccacagcaat acggtaaggt gcttgttgat
     9781 cgtaaattgt atcatcaaca tcaaaaacaa ttgaagtaat catgattatc cttttctttc
     9841 tgaattttca attgttaatt ataccttttt ctacccttat tttcaaatgg tgaaaagtct
     9901 attaaaaatg agaataacac cataaactca taaaataaat tatgaattat cagtgttttt
     9961 gtggtacaat aggatgaata ctaacttgga ggaaattatg tctaaccaac atattgaaga
    10021 attaaatgac caacagattg ttcgtcgtga aaaaatggct gctttgactg aacaaggcat
    10081 tgatcctttt ggtaaacgat ttgaacgtac agcaacatct ggtcaattaa atgaaaaata
    10141 cgctgacaaa tcaaaagaag acttgcacga cattgaagaa actgctacaa tcgctggtcg
    10201 tttaatgact aagcgcggta aggggaaagt tggctttgcg catatccaag atcgcgaagg
    10261 tcaaattcaa atttatgtcc gtaaggattc tgttggcgaa gaaaactacg aaatctttaa
    10321 aaaagctgat ttaggtgact tccttggtgt tgaagggcaa gtaatgcgta cagatatggg
    10381 agaactttct atcaaggcta ctcatattac tcacctttca aaagcactcc gtccacttcc
    10441 tgaaaaattc cacggcctaa ctgatattga aacaatctat cgtaaacgtc atcttgattt
    10501 aatctcaaat cgtgacagct ttgaccgttt tgtaacacgc tcaaaaatca tttcagaaat
    10561 tcgtcgcttt atggatagca atggtttctt agaggttgaa acacctgttc ttcataatga
    10621 agctggtggt gcttctgctc gcccatttat cacacatcac aatgcacaag acattgacat
    10681 ggttcttcgt atcgcaactg aattgcatct aaaacgtctt attgtaggtg gtatggagcg
    10741 tgtttatgaa atcggacgta ttttccgtaa tgaagggatg gatgcaaccc acaatccaga
    10801 gttcacatct attgaagcct accaagccta tgctgactac caggatatta tggacttgac
    10861 agaaggtatt atccagcatg ttacaaaaac tgtaaaaggt gatggaccaa tcaattatca
    10921 aggcacagaa attaaaatta atgaaccatt caaacgtgtt catatggttg atgcggttaa
    10981 agaaatcact gatattgact tctggaaaga aatgactctt gaagaagcac aagctttggc
    11041 acaagaaaaa aatgttcctc ttgaaaaaca tttcacaaca gttggtcaca tcattaacgc
    11101 cttcttcgaa gaattcgttg aagacacttt aattcaacca acttttgtct ttggtcatcc
    11161 agtcgaagtg tctccacttg ctaagaaaaa tgatacagac cctcgtttta cagaccgttt
    11221 cgaactcttt atcatgacta aagaatatgc taatgctttt acagaattaa atgacccaat
    11281 tgatcagcta tctcgctttg aagcacaagc atctgctaaa gaacttggcg atgatgaagc
    11341 aactggagtt gattacgatt atgttgaagc acttgaatat ggtatgccac ctactggtgg
    11401 actaggtatt ggtatcgacc gtctctgtat gctgttaact gacacaacaa ctatccgaga
    11461 cgtcctactt ttccctacaa tgaaataaga atatattaaa acccaccctt atttaaggtg
    11521 ggttttaata ttacctctac tgaactttgt taggtttctg atttatcgct cacgcaattt
    11581 tttatcaagg tttaccattt caatagccga aagagcacaa tcataacctt tatttcctgc
    11641 ttttgttcct gccctctcaa tagcttgttc gatattatcc gttgttagaa caccaaagat
    11701 atgagggata tctgactggc tatttaaatg accaatacct tttgtcacct cattacaaac
    11761 ataatcataa tgatcagtgc tgcctttaat gactacccca agacagataa tagcatcata
    11821 atctttatat aatgccattt ttctagccat ataggggatt tcaaaagcac cgggtaccca
    11881 tacaatatct atatcttctt ctgaaacacc atgtctcaac agtccatcta ctgctcctga
    11941 taataattta gatgtaatca attcattgaa acgtgagact acgattccta ttttcatttc
    12001 gtttgccact aattgtcctt caataattgt cattttttct tcctccttta atctaaaaga
    12061 tgtcccattt tttctttttt agtttttaaa taattaaggt cataagctgt tgctgccacc
    12121 tgtaatggca ctctgttttt gacgcaaatt ccttctgctt caagttgttg gattttatct
    12181 ggattattgg ttagtaagtt tatagaattt atacctaaat cttttaaaag ttgagcacta
    12241 actccatagt ctctctcatc tccttcaaaa cctaaagcca aattagcttc taaagtatct
    12301 aagccttctt cttgcaaatg ataagctttt aacttattaa tcaaaccaat tcctcgacct
    12361 tcctgtctca tataaagaag gacgccacga ccttcagcct ctatttgttg gagtgcatta
    12421 gccagttgtt ccccacaatc acatctcaag gaatgaaaga catcacctgt caaacattct
    12481 gagtgtaaac gacataaaac atcttcacca ctactaatat cccctttaac aagggcaaca
    12541 tgttccttat ttgccacaac ttcactatat ccataagcct taaaatggcc gaattgcgtt
    12601 ggtagctgaa tctcaatttc tctaaccacc cctccctctt cttgacgtct atagtcttgc
    12661 aattgtttta tggtagcgat agctaaatcg tgcttttgag caaatgctaa taactcatcc
    12721 tttctcatca tactaccatc ttcagccatt atttcacaac aaagtccaca ttcctttaat
    12781 ccagctaaac gacatagatc aacggttgct tcagtatgtc catttctagc taaaacacct
    12841 ccttccttag ctaacaaggg aaataagtgc ccaggacgtc taaagtcttt agctttgcta
    12901 ctgtcgttag caaccatctt agctgtcaaa gctctatctt ccgcagaaat cccagtacta
    12961 gttgatagat gatcaatgga tatggtaaaa gctgtttcat gattatcagt attatggcta
    13021 gccatttgag ctaattctaa cttttcagca tagtaattag acagaggtaa gcaaattaat
    13081 cctttcccaa atttagccat aaaattaatc gactcttttg tcaccatttc agccgcacaa
    13141 ataaggtccc cttcattctc tctattttcg tcatccatca aaactaccat ttcccctgat
    13201 ttgatgtctt gtaaaagttt ttttattgga ctaaaggcca ttcgctttct cctctctctt
    13261 agtagccatt ttctgataaa aaagaccaat ccattactga tgaagtgtga ttagttctat
    13321 tagtcggtgc taaaaatttc tcagcatatt tgcctaaaac atccacttcg atatttaata
    13381 aatccccaac cttttttaag gataaattag tttgagccat cgtgtgggga acgaccgaaa
    13441 cttcaaaaga atgctctgta aatgatacca ccgttaggct aatcccatct agagccaccg
    13501 aacctttctc tataatataa gacataatac tgggcggagc atcaattgaa aatctgacag
    13561 caatatcatc cttttggatt ttttctattt ttccaacccc atccacatgt ccaagaacaa
    13621 aatgcccccc taaccttgag tcacttctga gtgctaattc taagttaact ttagaatgaa
    13681 gtcgtaaagc agctaagctt gttcgcatca tactttctgg catgacgtct gctataaaag
    13741 ctttttcact tttgtcaagg actgtcaaac aaacaccatt aacagctacg ctatcccctt
    13801 ttcttaataa agggactagt ttgggaacat caatacttaa ttgttgggat tttatgccat
    13861 tccgaatacg actgacttga cccatttcct caataattcc tgtaaacata atgttctacc
    13921 tttccttcaa taacaacgtc agaatccata cgactaagtt caatatcttt tactctgaaa
    13981 gcttggttaa gccaatccaa gccttctcca gaaatagctg tttttgcttt cagacctcct
    14041 attattttgg gtgcaataaa aacaattagt ctgttaacaa taccagcttc taaagctgaa
    14101 aaatgtaaac tacttcctcc ttcaataaga aggctatcaa tcccttcttg tcctaaaata
    14161 gtcattaata ctttcaaatc cagctttcca tcttttcttg ggaccttaat caaacggcaa
    14221 cccatttctt ttaatgtttg ttgctgagca agattatctg aacatgttgc aatccatgtt
    14281 ggtattgttt tagcgctctt aactaggtaa gagtctaaag gtatcttcaa ctgactatca
    14341 catacgatcc tcacaagcgc ttcaccttta ggaatacggc aagttaaacg tggattatca
    14401 gctaatacag tattgatacc gaccataata gcactacatt tttgcctcaa tttctgaaca
    14461 gattgtcttg agtgctcatt ggatatccac ttagaatcgc ccgtctttgt agctatcttc
    14521 ccatctaaag tcatagcata tttcaaataa acaaaaggct gcttataggt catgtgaaaa
    14581 ataaatcttt cattcagagc atcgcattct tctcttagga taccaacttc aacatttaaa
    14641 ccttcttttc ttaacaaggc aatccctttt cctgaaacta aaggatttgg atctaaactt
    14701 ccaactacta cttttttgat accagattta ataagagctt cagtacaagg tggttgctta
    14761 cccacatgac agcatggttc taaagtcacg tacagagtac ttccagagat atcctcatca
    14821 gcatttttaa tagcctgtct ttccgcatgt aaatctccaa atcgtttatg ataacctttg
    14881 gaaattatac gatcatcttt aacaataaca gcacctacta atgggttggg agcaacgaac
    14941 cccatacctt tctcagcctc tttcaaggct aatgccatgt agtcttcttt tttcatcatt
    15001 tcctttttcc ttctaaaata aaaaacctta gcttctaaaa tagaaaacta aggggaggga
    15061 tgagaagtcg ttgattatac taattagcat tatattttag gtagcacaaa aaaactaaag
    15121 actccctata gaaaactata gggagtctct cattaatcca gcacaaataa actgcaagta
    15181 gcagacaatc actgaattct tctctcatcc agactttact gtcggtactg gaatcacacc
    15241 agttcatgct cgaaagctcg cggactatac cgccggttag gattttcacc tgaccccgaa
    15301 gaaattctat ttgacagtta ttttactaaa ctcattttta tttgtcaata aaaataatgt
    15361 gttttttatt ataaaataat aattaagtag ctcatctatt cttatcttta aaagacagta
    15421 tatctatacc ataaagcaat ctttcgaact aatca
//
LOCUS       DATRME010000045        15228 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRME010000045 DATRME010000000
VERSION     DATRME010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15228
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..372
                     /locus_tag="VBC23_001372"
     CDS             <1..372
                     /locus_tag="VBC23_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6843316.1"
                     /translation="KVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEM
                     TSSKINTGMMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLG
                     MLSLAFATGLTALGLKKSKQR"
     gene            485..1171
                     /locus_tag="VBC23_001373"
     CDS             485..1171
                     /locus_tag="VBC23_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6843317.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            1173..2210
                     /locus_tag="VBC23_001374"
     CDS             1173..2210
                     /locus_tag="VBC23_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6843318.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(2224..2964)
                     /locus_tag="VBC23_001375"
     CDS             complement(2224..2964)
                     /locus_tag="VBC23_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6843319.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISFYYQYN
                     NMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            3151..4293
                     /gene="tgt"
                     /locus_tag="VBC23_001376"
     CDS             3151..4293
                     /gene="tgt"
                     /locus_tag="VBC23_001376"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6843320.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            4399..4710
                     /locus_tag="VBC23_001377"
     CDS             4399..4710
                     /locus_tag="VBC23_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843321.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFVATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            4717..5256
                     /locus_tag="VBC23_001378"
     CDS             4717..5256
                     /locus_tag="VBC23_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6843322.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            5395..6171
                     /locus_tag="VBC23_001379"
     CDS             5395..6171
                     /locus_tag="VBC23_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6843323.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            6171..6677
                     /locus_tag="VBC23_001380"
     CDS             6171..6677
                     /locus_tag="VBC23_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6843324.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            6712..6797
                     /gene="ffs"
                     /locus_tag="VBC23_001381"
     ncRNA           6712..6797
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBC23_001381"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6949..8298
                     /locus_tag="VBC23_001382"
     CDS             6949..8298
                     /locus_tag="VBC23_001382"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6843325.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            8620..9147
                     /locus_tag="VBC23_001383"
     CDS             8620..9147
                     /locus_tag="VBC23_001383"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6843326.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            9138..9815
                     /locus_tag="VBC23_001384"
     CDS             9138..9815
                     /locus_tag="VBC23_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6843327.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            9947..10990
                     /locus_tag="VBC23_001385"
     CDS             9947..10990
                     /locus_tag="VBC23_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6843328.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(11081..11980)
                     /gene="galU"
                     /locus_tag="VBC23_001386"
     CDS             complement(11081..11980)
                     /gene="galU"
                     /locus_tag="VBC23_001386"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6843329.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(12017..13033)
                     /locus_tag="VBC23_001387"
     CDS             complement(12017..13033)
                     /locus_tag="VBC23_001387"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6843330.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            13203..13532
                     /gene="rnpA"
                     /locus_tag="VBC23_001388"
     CDS             13203..13532
                     /gene="rnpA"
                     /locus_tag="VBC23_001388"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6843331.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            13545..14360
                     /locus_tag="VBC23_001389"
     CDS             13545..14360
                     /locus_tag="VBC23_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6843332.1"
                     /translation="MKKKFKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            14368..15189
                     /locus_tag="VBC23_001390"
     CDS             14368..15189
                     /locus_tag="VBC23_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6843333.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFWGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         5102 a         2342 c         2909 g         4875 t
ORIGIN      
        1 aaagttgata tgaatactat tttaggacaa aagacagtga tgattcatat taatgtcgct
       61 aaaaaagata tgaatagcac atcaatgatg atgaaaaaag ataagatgac aatgcctatg
      121 aagaaagaaa tgacttcatc taaaataaat accggtatga tgatgtctaa caacaagatg
      181 tcagctaata tgcaaatgtc atcacaagcc aaatcaaatg ataaagctgg taaaaaaatg
      241 tctatgatgt ctaaaaattt acctaataca ggtgaaacaa agcaacaaaa tgtaggtgtg
      301 ctaggtatgc ttagtctagc ctttgcgaca ggtctaactg cgctaggcct taaaaaatct
      361 aaacaaagat agggatagta tttaattcag aacaccaaat ttgtttaaaa ttattgaggg
      421 ttatcgtggt aaaatagtgt tatactagag gtatagcact attttgactc attaggagag
      481 tagaatgaca caaaaattat tattagttga cgatgaattt gaaattattg atattaatag
      541 acgttatcta gagcaagcag gatatgaagt gtcagtagct gcggatggca tagaagcact
      601 caaagaggta gatgagaatc gttttgacct aattattagt gatattatga tgcctaaaat
      661 ggatggctat gattttatta gtgaagttct agttcgagaa cccaaccaac cttttctctt
      721 tataaccgct aaagtatctg aaccagataa aatttactct ttgagtatgg gtgcagatga
      781 ctttatttcc aagcctttca gtcctaggga attagtttta cgtgttaaaa atattctaag
      841 acgtatctat gggaatcatc aacaatctga agtattgacg attggtgatt tggttatcga
      901 ccaaaaacaa cgtttggtaa tggtagactg caatacaatt tccttaacca ataaatcatt
      961 tgacttgctt tggatattag caaatcattt gaatcgtgtt ttttcaaaaa cagagcttta
     1021 tgaacgtgtc tggggcgaag aatttttaga tgacactaat actttgaatg ttcatatcca
     1081 tgccttacgc aatgaccttg ctaaatttag tacagacaat accccaacta tcaaaacagt
     1141 ttggggatta gggtataaat tagaggaata aaatgaaatt aaaatattat attgttattg
     1201 gttaccttat ttctatgtta ataactgtag caggtgtttt ctttgggctt aatcatatgc
     1261 tgattgagac tcgaggtgtt tactacatcc tttcagttac aattattgcc tgtatcgtag
     1321 gtggtatagt taatttgttc ttattatcaa gtgtatttac atctttgaaa aagttaaaac
     1381 aaaaaatgaa ggatatttct caaagacgtt ttgatacaaa agcacaaatt tgttcaccac
     1441 aagaattcaa ggatttagag acagccttta accaaatgtc ttcagagtta gagtcaacgt
     1501 ttaaatctct aaacgagagt gaacgcgaaa aaacaatgat gattgcacaa ctttcacatg
     1561 atatcaagac tccaattacc tctatacaat caacagtaga aggtatttta gatggtatta
     1621 tatctgaaga agaagttaac tattatctca ataccatttc tcgtcaaaca aaccgtctta
     1681 accatctagt tgaagaatta agttttatta cattagagac tatgtcagac acggctgaac
     1741 ctcataaaga agaaacaatt tatctggata aactactgat tgatatttta tctgaatttc
     1801 aattggtgtt tgaaaaagaa aatcggcaag taatgattga tgttgcgcca gatgtttcga
     1861 aattatctag tcaatatgat aaattatcac gaattttatt gaatttgatt agcaatgcag
     1921 tcaaatattc tgatcctgga agtcccttga ctatcaaggc ctattcaaat cgtcaagata
     1981 tcgttatcga tattattgac caaggctatg ggataaaaga tgaagatctt gcttctattt
     2041 ttaatcgttt ttatcgtgta gaatcttcac gaaatatgaa gacaggagga catggcctag
     2101 gtctttatat tgcgcgacaa cttgcacatc aactgaatgg tgatattttg gtcgaaagtc
     2161 agtatcaaaa aggcagtaag ttctctttag tacttaaatt acaaaaatag ctaggaatta
     2221 ttcctagcta ttttttgtaa tgtttctata aaagattaag cgcgcagtag tttggtaagg
     2281 aaggagatag atatagacta atccgaaact gaagattgta aggaaatgcc aaccaataaa
     2341 acttaagtct aaaaggaaga gtctaagttt attaccattc attagttctc gactagcaac
     2401 tagtacttta gatggtccct catagtttcc actttctaat tgagtgtaaa atactaaagt
     2461 tgagatacta taggagtaat attgttgaac gagttttata actgccttag tagtaattag
     2521 gctactaaca actattaaaa caggggcaag gataaggtta ccactcaaaa gtagcctaaa
     2581 acttgaaatg atgattagta gaccaaaagt acttagtaga taaaagaaag ataaaattag
     2641 tccttttaga acaagtactg agagtaattt ccagaaaata tggcttgaaa atacataagt
     2701 attgtccgag aatctaacgt ttagtttctg atttttgacg acatctaaca ttacaaaact
     2761 gactgaaata ataaaaagtg tgtaaaagaa ataaactgga agaggtacaa aaaaatcaag
     2821 tagtgtcata ttattatatt gataataaaa tgatatatat aacgtaataa tactaagaag
     2881 aatcgggagt aaaaaaagtt gattcttacc ttgaagattg cttaatatag tttttgcctc
     2941 atttttaatt tctgaattcg tcatatctcc tccatacata aaagtacaat tcatttcatt
     3001 ataacacatt ccttaagatg agcatgacaa gagtacattt ttttgataaa atcttaatgg
     3061 ctaaaaaaga aattaataca aagtattaca tttagatatt gattagcact aaagcccatg
     3121 actgtttttc atggaagcaa ggagaaaacg atgactgatc atccaataaa atatcgatta
     3181 attaaacagg aaaaacacac aggagccaga cttggtgaaa taatcacacc acatggtaca
     3241 tttccgacac caatgtttat gcctgttggg acacaagcaa ctgttaagac acaatctcct
     3301 gaagaactaa aagaaatggg ttcaggaatt attctatcaa atacctacca tttatggtta
     3361 cgtccaggtg atgaattgat tgctaaagct ggaggtttac ataaattcat gaactgggat
     3421 caggctattc taacagacag tggaggcttt caggtttatt ctttggctga tagtcgaaac
     3481 atcactgaag aaggagtaac ctttaaaaac catttaaatg gtgcgaaaat gtttttatca
     3541 ccagaaaaag ctatttcaat tcaaaataac cttggttctg atattatgat gagttttgat
     3601 gagtgtccac aattttacca gccttatgat tatgtaaaaa aatcaattga gcgaacaagc
     3661 cgttgggcag aacgtggatt aaatgcccac cgccgtcctc atgatcaagg actatttggt
     3721 attgttcaag gggcagggtt tgaggattta cgtcgtcaat cagcacgaga tttagtatcg
     3781 atggattttc caggttattc aattggaggg cttgcggtcg gagaaacaca tgatgagatg
     3841 aatgcagttc ttgattttac tgttccaatg cttccgaatg ataagcctcg ttatttgatg
     3901 ggagttggtg caccagatag tttaatagac gccgttatcc gaggagtaga tatgtttgac
     3961 tgcgtcttac ctactcgtat tgctcgaaat gggacatgta tgacaagtca aggtcgttta
     4021 gttgttaaga atgctaaatt tgcagaagat tttacgcctc ttgatccaaa ttgtgattgc
     4081 tatacttgta aaaattatac acgcgcttat atccgtcact tattgaaagc agatgaaact
     4141 tttggtattc gtttgactag ttaccataat ctttatttcc ttgtcaatct tatgaaagat
     4201 gtcagacaag ctattatgga tgataatctt ctagagttcc gtcaagattt tatggagcgc
     4261 tatggttatg gcacgaataa ccgtaatttt taaaaaagtt cctggtaaaa cagggctttt
     4321 tttgatctgt ttgttaaccg cgttttaaaa aaagttgaca atagaaaaac ttttgtataa
     4381 tatgttatag gaggaatcat gatgcaagag tactttggta ttggtcttga tagtagcagc
     4441 cgctgttacc actatcatac taaattggat attgtcgctt taaaatgtgc tgtttgtcaa
     4501 aaatattatg cttgttataa atgtcatgat gcactcgagg agcattgttt tgtagcaaca
     4561 aaatctgatg aaatgtttcc tgtcttatgc ggttcttgtc gtcagatgtt gacattaaaa
     4621 gagtataaaa ctggtttttg tccttattgt cgtatgcttt ttaatccaaa ttgtcaacgc
     4681 catgattcta tttatttttc gaaggagtaa tttttaatgt ttcacacaaa aagcctaatc
     4741 cagatggcta tgatggctac tttattggtg gtacttggtt tcattcctgc tctaccactt
     4801 ggtttcattc cggtccctat cgtcttacaa aacctagggg ttatgatggc tggtgttatt
     4861 ttgggcggaa gaaaagggag tttatctatt ttgctcttct ttcttgtcgg tttagttatt
     4921 cctgtattct caggtttacg ttcgactata cctgtattga caggaccaac agctggttat
     4981 gttattgctt ggttgtttgt tcctttgctt atctcatatg gtgtaaaatt agtaaataaa
     5041 aagaatttta tagctatttt ccttattgta tggctagcag gggtcctttt tgtagatgta
     5101 gcaggagcag tttggctggc atcttatatg aacatcccat tagataaatc acttcttcaa
     5161 aacttagtat ttctcccagg agatacaata aaagccatta tagctgctgt tgttgctgtt
     5221 aaatataaag atagtttttt cttgactgaa aaataatcaa gattgctata ttttagatgg
     5281 actagtagat ctaaaaattt agatttacta gtcttttttg tgcataaaat ctaaaacagt
     5341 tattcttaac aaaatttggt ataatagcat taaatcagta aggaggggat tatcatgaaa
     5401 ttaacaacgt taggttgttg gggtggttat ccttataaag atggaggaac cacaagtttt
     5461 ctattaactg gagaaaataa ttttcactta ttaatagatg cgggaagtcg ttcaatcaat
     5521 gagttagaaa aagaaataag cccgttggat ttggatgcag ttatcatcag tcattatcat
     5581 ccagatcatg tagcagattt gggtgtgttg tatcattatc gtttgctata ccctaaagat
     5641 aaatgggaac ccaaagtatt gccagtttat ggacataatg aggatgatta tgagttttct
     5701 aaattgacat tacctaacgt tgctcaagga attgcttatc ataccaatgg agttgagcaa
     5761 gttggtccct tcaatattac gtttatcaaa acagtccacc cagtcgtctg ctatgccatg
     5821 aggattacag aaagagaaac tggtcaagta ttagtcttta caggagattc tgggtatttt
     5881 gctgaccttg taccttttac aaaaggcgca gatttatttt tagcagacgt ttatctttac
     5941 gagggaaatg aaaatcatat tgcacatttg actagtaaag aagcaggtga gattgcttat
     6001 aacgctaaag ttaagaaatt agttttaaca catgttccgc agtttccggg agaaggaata
     6061 aaccccgaaa atcatttgga agaacttcga ctacagtctg aaaagtatgc aggtggcatc
     6121 tctgttgatt tagctcagcc acataaatct tgggacttag gaaaactata atgcagaaat
     6181 ttacccaaga agaaaaagaa tggtttatga cagaagctct aaaggaggca aaatcctcct
     6241 tagagaaaga agaaattcct ataggttgtg tcattgtaaa agatggtcat attattggaa
     6301 gaggacataa tgctcgtgaa gagtttaata aagctatttt acatgctgaa atcatggcta
     6361 ttaacaatgc aaatgagaag gtagggaatt ggcgcttatt agatactact ttatttgtta
     6421 ctgtagaacc ttgtgtaatg tgtagtggag ctattggctt agctagaatt ccccacgtta
     6481 tatatggcgc taagaatacg aaatttggag cagcgggaag tctctatgat attttaagtg
     6541 atagccgact taatcataga gttgaagttg aaaccggcat tttagaagat caatgcgcta
     6601 aaataatgca ggatttcttt cgaaaaggac gacaacgcaa gaaagaagct aaactaagtg
     6661 aaaaaactaa gatttgatag cttgcaataa aatattagtt ttgttataat atctttggag
     6721 caacatttac tcgtgaagtg ggtcagggga ggaatccagc agccctaagc gatgctaaat
     6781 gtgtgctctt tttattttca aaagtcaaag tgtttttgga aataattatg aaagtttcat
     6841 aaaaattggt agcgtttcaa aaaatatgaa aatacagtta aaaaaccttg aaaaaaaact
     6901 cacaaaagag taagatgata gtgaaacata attgttagga gaaataaaat gacacatatt
     6961 acatttgact attcaaaagt cttgggtcaa tttgtaggcg aacacgagtt agactaccta
     7021 caaccacaag taagtgcagc agatgctttc cttcgtcaag ggactggtcc tggatcagat
     7081 tttctcggat ggatggacct cccagaaaac tatgacaaag aagaattttc tcgcattcaa
     7141 aaagccgctg aaaagattaa atcagatagc gaagtactcg tggttattgg tattggtggt
     7201 tcgtaccttg gtgcaaaagc agcaattgac tttttgaata atcattttgc taatttgcaa
     7261 accgcagaag aacgtaaagc gcctcagatt ctttatgctg gaaattctat ttcatctact
     7321 taccttgccg atttagttga atacgtccaa gataaagaat tctcagtaaa tgtcatttca
     7381 aaatcaggta caacaactga accagcgatt gctttccgtg tatttaaaga acttctagtt
     7441 aaaaagtacg gtcaagaaga agctaataaa cgtatctacg ctacaactga taaagttaaa
     7501 ggtgccgtaa aagttgaagc agatgctaac aattgggaaa catttgtagt tcctgataat
     7561 gttggtggtc gtttctcagt actgacagct gtaggtcttc ttccaattgc tgcttcagga
     7621 gcagatatta ctgctcttat ggaaggcgca aatgctgctc gtaaagattt atcttcagat
     7681 aaaatctctg aaaacattgc ttatcagtac gctgcagttc gtaatgttct ttatcgcaaa
     7741 ggttatatca cagagatttt agctaactac gaaccatcat tacaatactt tggtgaatgg
     7801 tggaaacaat tagcaggtga atcagaaggt aaagatcaaa aaggtatcta tcctacatca
     7861 gcaaacttct caactgatct tcactcactt ggtcaattta ttcaagaagg ttaccgtaac
     7921 ctctttgaga cagttgttcg cgttgagaaa cctcgtaaaa atgtgactat tcctgaatta
     7981 accgaagatt tagatggtct tggttatctt caaggaaaag atgtagattt tgttaataaa
     8041 aaagcaacag atggtgtgct tcttgctcat acagatggtg gggttccaaa tatgtttgta
     8101 acgcttccta cacaagacgc ttacactctt ggttacacta tttacttctt tgagttagca
     8161 attggccttt caggttatct taactcagta aatccatttg atcaaccggg ggtagaagca
     8221 tataaacgta atatgtttgc tcttctaggt aaacctggat tcgaagagct tagcgctgaa
     8281 ttgaatgcac gtctttaaaa atatctaact aaaaagttcc tcaaagagag gaacttttta
     8341 gttttgttgt aggataacta aaaacccact agcactggta gcgatagtgg gttggaaaaa
     8401 gggttgagaa aagttattaa cttttctcag gagatttatg aaaaaggaaa ataatttagg
     8461 ataatatttt cctaacggct aggataaacc gcttatttta ggatgatgct agtatacagt
     8521 agaaacctta aataaacctt aaaaattcat actttatcat tttattttcc cctatactat
     8581 ttttgtataa atggtcaata ttaatggtat catagatata tggaaaagaa attattaaga
     8641 aaagaagttt tgataacctt aaaatctcaa cctcaagctt acaaatcaga agttgactct
     8701 aaacttttag aggcttttat aaaaactaaa gcttatcaaa atagttgtgt aattgctact
     8761 tacctatctt ttgactacga atacaataca caacttctta ttaaacaagc tttatgtgat
     8821 ggcaaacgtg tccttgttcc caaaacttat ccaaaaggta aaatgatttt tgtggactat
     8881 caaaaagata acttgagggc aacaccgttt ggcttattag aacctgttaa tgatagagct
     8941 gttgaaaaag ctagtataga cttaattcat gtgcccggac ttatctttaa caataaaggt
     9001 tttcgtatag gttatggtgc aggttatttt gatcgctatt tgagtgattt tgaaggtgat
     9061 actattagta ctatttatag gtgtcagaga caggattttg tagaagagaa gcatgatgta
     9121 gcggttaagg aggtattatg tctatgaaga agttcgctaa agagtatccg acgacagtac
     9181 tcttggtcgg tctgacaacc cttgtttttt tattgatgca actaacttat ggaagtcaag
     9241 cagagtctag ccaagttatt tttcaatttg gtggaataca aggtgattat ctaaaagcat
     9301 atccaacaaa tctatggaga ctaataagtc ctatctttgt tcatattggt tgggaacatt
     9361 ttttattaaa tggtttagct ctgtattttg tgggacaaat gggggaaagt atttggggaa
     9421 gtttgagatt tctaattctc tatattttat ctggcttaat gggtaatatt tttacgctat
     9481 tttttactcc acacgtggtg gcagcaggag cctcaacgtc tttatttggt gttttttctg
     9541 ctatagcaat cgcaggttat tttggaaaaa atccttattt aaaacaagtt ggaaaatcat
     9601 atcaagtgat gatcttgtta aatttatttt ttaatatctt cacacccgga gtgagtttag
     9661 caggccatgt tggtggttta gttggtggag tattggtggc tatttttttg acaaaacaaa
     9721 atggttcttt attatttaaa acttggcaaa gtattttagc tttaatgata tttattattg
     9781 tatcgatcag tttaataggt ctatcgttgg tataattcta cggctatagt ctctaaaacc
     9841 aaactttctt tgggaaaaat agaataaagt ttgctaaatg atttttttat gttatgatag
     9901 aaatctgttt ttatgtgact ataacttatt aggaggttca ttctcggtga acagaaaaat
     9961 tattggctta actctactat ctttatctgt actaacatta acagcatgtg gtaatcgttc
    10021 agataagtct gcaaataaat cagatatcaa agttgcgatg gtaactaatc aaggtggagt
    10081 agacgataaa tcgtttaatc aatcagcttg ggaagggcta caaaagtggg gaaaaaagaa
    10141 ggggttaaca aaaggtaatg gctttgatta tttccagtca tctaatgaat cagatcatgc
    10201 taataacctt gatacggcag cctctagtgg ctataattta atttttggta ttggatttgg
    10261 tattcatgat actattgaaa aagtatcaga aaataataaa gatgttaaat acgttattgt
    10321 agatgatatc attaaaggta aagaaaatgt agctagtgtt acttttgcag acaatgaagc
    10381 tgcttactta gctggagttg ctgcagctaa gacaactaag acaaaaacag ttggttttat
    10441 cggagggatg gaaggcgtcg ttgttaagcg atttgaagca ggatttaagg ctggagtaaa
    10501 atcaattgat ccggctatta aagttgcggt atcttatgca ggctcattta cagatgcagc
    10561 taaaggtaaa acgattgctg ccacacagta tgcaacaggt gttgatgtta tttaccaggc
    10621 tgctggtggt acaggagcag gaatttttag tgaggctaaa actgaaaatg agacccgcaa
    10681 ggaatctaac aaagtctggg taattggtgt tgacagagat caaagtcaag aaggcaatta
    10741 tgtttcaaaa gatggtaaga aagcaaactt tgttctggca tctactataa aagaggtggg
    10801 gaaatctctc caatctgtag cagaattaac agagaaaaaa caatatccag gagggaaagt
    10861 aacagttttt ggtctaaagg atagtggtgt agatattaaa gagcatcaat tgtctagtga
    10921 gggatctgtt gctgtcaaaa aagctaaaga ggggattgtt tcagggaaaa tacaagttcc
    10981 tatgaaatga taaaagtgtg gaggaacaat aagttcttcg tcaagtacaa taaaaaagtt
    11041 tcctaaatag gaaacttttt tattgtactt gactcatatt ttatttctta gatgtttttt
    11101 ctagagattt tcctaaatca atgatatatt tcttcaagtc atctttaact tgtgggtgtt
    11161 gaagagcata atcaatagaa gttttcataa aaccaaactt gtcaccaacg tcataacggt
    11221 cccctgtaaa ttttctagca aaaacacgtt gcgttttatt taaagtatcg atagcatcgg
    11281 ttagttgaat ctcatttcct gcaccaggtt tttgagtttc taaaatatta aaaatttctg
    11341 gagttaacaa gtagcgacca atgattgcca aattacttgg agcctcttcg ggagaaggtt
    11401 tttcaacgaa agtgttaacg ctatatagac cattaactcc ttctccttgt ggagcaataa
    11461 caccataagc agaaacatct tcatgtggaa cttccataac agcaattgtt gatgcatgag
    11521 tagcttcaaa gtcattaatt aattgtttgg tgagaggaat aactttatta ttggtaatat
    11581 ccattagatc atcacctagc ataacaacaa aaggttcatt accaacgaag gcttttgctt
    11641 gtagcacagc atctccaagc ccacgaggat gactttgtcg aataaaatgg agtcgaatgc
    11701 cagttgtttc atcaacaagt ttgagaagtt cgtttttccc tttctctttt aggttatatt
    11761 ctaattcaaa atttgaatca aagtggtctt ctatagaacg ttttgattta ccagttacaa
    11821 ctaaaatatc ttcaattcca gattttagtg cttcttcaac aataaattga atagttggtt
    11881 tatcaacgat tggaagcatt tctttagcta aagctttagt agcaggtaaa aaacgtgttc
    11941 ctaaaccagc ggcagggata actgcttttc tgactttcat atatagctcc tttaaatagt
    12001 attaatataa acgatattag tgccattcat tctctgaacg aaattcattg gacatcatat
    12061 ctaaaatgct atctttgata ttagcccctt catatattga tttataaata gcttctgtaa
    12121 tgggcatgta aacatttaag ttttgggcga tttcgtaagc aaccttagtt gttgagatac
    12181 cttcaataac catccccata tttttttcaa tatcttcaag tttctcacca cgtcctaaag
    12241 catctcccgc tctccagttg cgagaatgaa cagatgtacc agtcacaatt aaatctccaa
    12301 ctccggataa accactgaaa gtgagagggt cggcccctaa ttgtacacca aggcgtgtta
    12361 tctcagctaa accacgcgta ataatagctg ctttagcatt atcaccataa ccaagaccgt
    12421 gtagtgctcc agcaccaaca gcaataatat tttttagagc accagctgtt tctactccta
    12481 cgacatcagt gttagtataa agtctaaaat aatgattact aaacaatttt tggacatact
    12541 tagctgcttc aatatctttt gaagcagcag taatcaaagt aatatcacga acgatggctt
    12601 cttcagcatg actgggacct gaaacaacaa cgatatcact acgatattgt tcagagattt
    12661 cttcttctaa aattgtagaa agtcgttcat gagtgccagg ttctagcccc ttagaagcat
    12721 gcataattac aactttatgc tttaaaagac tagccacttg tttagcaaca agtcttgtaa
    12781 ctttagttgg gacgacgaat agaatgctgt caacattatt aatcgcttcc tcaagattag
    12841 tgtaggcttt tattttggaa tctaaggtaa tatctttgaa gtaacgttga ttagtatggt
    12901 tagtattaat ctcttctatt tgttcaacca cgtttcccca taagcgtacc tcatgtccat
    12961 tgtcatttaa tacttgtgca agagcagtcc cccatgaacc aggtccaaga acggcaattt
    13021 tttgttttgt catcttattc cttctttcta ataataaggc atatagcgct aacattatat
    13081 catactctaa tacactttgt cttgtttagt ctgttgtcga tgatagtagc attagaaaag
    13141 aattgagtct tttaggttat ttatggtata ataaaaccaa tttagataaa tggagcacta
    13201 ttttgaaaaa aacctatcgg gtaaaaagtg ataaggattt tcaaatgatt ttcagcagag
    13261 gtaaaaatgt tgctaatcga aaatttgtta tctactattt agaaaaagaa caaaaacact
    13321 ttcgtgtggg aatctctgtt agtaagaaat taggaaatgc tgttgttcgt aacgctgtta
    13381 aaagaaaaat taggcatgtt ttactctctc agaaaacagc gctgcaagat tatgattttg
    13441 tcgtgattgc gcgtaaaggt gtagaggaat tagactatca agcattagaa aaaaatctta
    13501 tacacgtttt aaaaatagca ggactcatct agaaaggtat aaaattgaag aaaaaattta
    13561 aaacatttag cttaatattg ctgacaggtt ctttattggt tgcttgcgga cgaggagaag
    13621 tatctagtca ttcagctaca ttatgggagc aaatagttta tgcatttgct aaatcaatac
    13681 aatggctctc ctttaaccat tcaattggtt tgggaataat cttattcact ctaataatca
    13741 gagcgattat gatgccgctt tataatatgc aaatgaaatc cagccaaaag atgcaggaaa
    13801 ttcagccaag gctcaaggaa ttacaaaaaa aatatccagg gaaagatcct gatagccgtt
    13861 taaagctaaa tgatgaaatg caatctatgt ataaagctga gggagttaac ccatatgcaa
    13921 gtgtattacc tcttctaatt cagttacctg tcctatgggc tcttttccaa gctttgacta
    13981 gggtttcttt cttaaaagta ggaacgtttt tgagcctaga attatcacag ccggacccat
    14041 attatatttt acctgtactg gcggccttat ttaccttttt atcaacttgg ttaactaaca
    14101 aagcagctgt tgaaaaaaat attgctttaa ctcttatgac ttatgttatg ccgtttatca
    14161 tattggtaac aagttttaac tttgctagtg gtgttgtttt atactggacg gtgtctaatg
    14221 cttttcaagt atttcaaatt ctattattga acaacccata caagattata aaggttcgag
    14281 aagaagcagt aagagtggca cacgaaaaag aacaaagagt taaacgtgct aagagaaaag
    14341 caagtaagaa aagaaagtag gaaaaacatg gtattattta caggagcgac tgtagaagaa
    14401 gctattgaaa aaggactaca agagttaaac atttcacgat tgagggcaca tataaaagtt
    14461 gtatcacgtg agaaaaaagg tttttggggc tttggtaaaa agccagcaaa agttgaaata
    14521 gaaggtatca ccgacgaagt aactgatatt aatgaatcgg tagcgttaaa aaatattaag
    14581 aatgtaccgt ctagcgtaga tgtggtggaa gaatatatag aggaggttga tgaaacgctt
    14641 gaaaaagaag acgtatcaca accagaactt ccaaaaattg atgataaaaa cgttgtaact
    14701 acaagtgaag ctattgaaaa aattgaccta cttcctaata ttgaagtagc agctgctcag
    14761 gtgactaaat acgttgaaaa tatcatctat gagatggatt tagatgcaac aatagaaact
    14821 acgacaagta agcgacaaat aaatctccaa atagaaacgc cagaagctgg gcgcatcatt
    14881 ggttatcatg ggaaagtatt aaaatcgttg cagttgttag cacaaaatta tcttcatgac
    14941 cgcttttcta agagtttttc agtatctatt aatgtccatg actacgttga gcatcgtata
    15001 gaaactttaa ttgatttttc taaaaaaata gctagacgtg ttttagagac taatgagcct
    15061 taccatatgg atcctatgag taacagtgag cgaaaaactg ttcataaaac aattgcaact
    15121 atagagggtg ttgaaagtta ttctgaaggt aacgatccta atagatttgt agtagttact
    15181 aagaagtaaa aagaggtttc acctcttttt ttcttgcgcc cgatctac
//
LOCUS       DATRME010000046        15114 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRME010000046 DATRME010000000
VERSION     DATRME010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15114
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(8..1069)
                     /locus_tag="VBC23_001391"
     CDS             complement(8..1069)
                     /locus_tag="VBC23_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO6843334.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            1368..2645
                     /gene="serS"
                     /locus_tag="VBC23_001392"
     CDS             1368..2645
                     /gene="serS"
                     /locus_tag="VBC23_001392"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6843335.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            complement(2691..3323)
                     /locus_tag="VBC23_001393"
     CDS             complement(2691..3323)
                     /locus_tag="VBC23_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6843336.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(3791..4564)
                     /locus_tag="VBC23_001394"
     CDS             complement(3791..4564)
                     /locus_tag="VBC23_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6843337.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            complement(4557..5432)
                     /locus_tag="VBC23_001395"
     CDS             complement(4557..5432)
                     /locus_tag="VBC23_001395"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6843338.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            complement(5441..6811)
                     /gene="accC"
                     /locus_tag="VBC23_001396"
     CDS             complement(5441..6811)
                     /gene="accC"
                     /locus_tag="VBC23_001396"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6843339.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            complement(6849..7271)
                     /gene="fabZ"
                     /locus_tag="VBC23_001397"
     CDS             complement(6849..7271)
                     /gene="fabZ"
                     /locus_tag="VBC23_001397"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6843340.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            complement(7268..7804)
                     /locus_tag="VBC23_001398"
     CDS             complement(7268..7804)
                     /locus_tag="VBC23_001398"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6843341.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            complement(7770..9002)
                     /gene="fabF"
                     /locus_tag="VBC23_001399"
     CDS             complement(7770..9002)
                     /gene="fabF"
                     /locus_tag="VBC23_001399"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6843342.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            complement(9018..9752)
                     /gene="fabG"
                     /locus_tag="VBC23_001400"
     CDS             complement(9018..9752)
                     /gene="fabG"
                     /locus_tag="VBC23_001400"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6843343.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            complement(9761..10687)
                     /gene="fabD"
                     /locus_tag="VBC23_001401"
     CDS             complement(9761..10687)
                     /gene="fabD"
                     /locus_tag="VBC23_001401"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6843344.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            complement(10707..11666)
                     /gene="fabK"
                     /locus_tag="VBC23_001402"
     CDS             complement(10707..11666)
                     /gene="fabK"
                     /locus_tag="VBC23_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6843345.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            complement(11821..12045)
                     /locus_tag="VBC23_001403"
     CDS             complement(11821..12045)
                     /locus_tag="VBC23_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6843346.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            complement(12103..13074)
                     /locus_tag="VBC23_001404"
     CDS             complement(12103..13074)
                     /locus_tag="VBC23_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6843347.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            complement(13074..13508)
                     /locus_tag="VBC23_001405"
     CDS             complement(13074..13508)
                     /locus_tag="VBC23_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6843348.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            complement(13604..14395)
                     /locus_tag="VBC23_001406"
     CDS             complement(13604..14395)
                     /locus_tag="VBC23_001406"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6843349.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            complement(14534..>15114)
                     /locus_tag="VBC23_001407"
     CDS             complement(14534..>15114)
                     /locus_tag="VBC23_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6843350.1"
                     /translation="RRERFLKQHGITINHLPMNFFIGGNMKQVWKSVLGDQYDTWDID
                     KLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHHKGYLLGLASSSTRHDIMLALESFN
                     LDTYFKVILSGEEFSESKPNPAIYNRAAELLDIPKQQILIVEDSEKGIAAGIAAGIDV
                     WAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
BASE COUNT         4788 a         3229 c         2291 g         4806 t
ORIGIN      
        1 tctaatatta atatgggaac ttgtcatcaa accatctcaa aaagttagaa aaatggattg
       61 ctttaaatat tcgtaatcta atacgttcta caataaaagc caacaaataa attaaaatcc
      121 ctgctcctaa aattaaaaat gggtaaagat aaatggactc ataaacaaac gtctctgcaa
      181 aatctcttac aaaatatttt actattagtg ggtggagatg acagagataa actcccaagg
      241 ttgaaggggc taatacaaca attattttct ttaagaatcc cgagggctta atgcttgctc
      301 tagcaaataa tataaacaaa cttaccgccc ctaaagttaa agtaggcgaa acataccaat
      361 accaaatatc accaatacta aacttcatga cataagtagc aaccaatgat aatagataaa
      421 ctataagtaa gtatgatgtt ttaaatatat ttaaatcaat acgtttcaaa taggcaccaa
      481 taatatataa aatcagtagc caagtcattt caaaaccctt gctcaatgaa aattctggaa
      541 cacgattgtt taaaacagct ggtaaaattg aaaaaataat aaacattaac aaaaccaatt
      601 gttttaattg tttatcagtc aaggcattta atccattatt aataacaggc ataaaaacca
      661 atagaccaaa gtaagcagta atgtaccaat actgcccact gacaataggg aaaaaagcat
      721 ctctccaatt aagcaaggtc acttcatgac ctgtgattgc aaaaagtgct gttattgtaa
      781 aggtgtagaa aaaaacctgt gtccagatag acagtaattt tgagtaacga taacgcgaat
      841 taattcccac ataaccactt attagtgcgt aacagttaac ggccccataa actaagactt
      901 ggataatcca cgttactata aaataggagt ctgcgtgtcc ctcaaccgaa gatctcagac
      961 caccttttcc aagaacatgc gtaataacaa tcatgaacat ggaaataatc cttaaaaggt
     1021 ctaagccgta atgacgacct gattgtttaa tacttgtata cttttccata atcatatttt
     1081 agcatagcta tttcactaaa acaaagagca ggcttataaa gttaaggttt acattttact
     1141 tatttttgga tataataacc ttaacggata agtagtataa tagttctttt cagagagtct
     1201 gcggtcgttg cgagcagata agattgtttt atgaaattct accgtgttcc atttatggga
     1261 aataatttaa tcaatcaatc aagtgcactt ctgtgaagct ggatggaacc gcgcctttgc
     1321 gctccagcat gacagggtgt tttttttagt ataaaggaga ttcctatatg ttagacttaa
     1381 aacgtattcg tacagatttt gatgctgtcg ctaaaaaatt ggccacgcgt ggtgttgacc
     1441 aagaaacatt aacaaccctt aaagaacttg atataaaacg ccgtgaatta cttatcaaat
     1501 ctgaagaggc gaaagcgcaa cgtaatgttg cttctgctgc cattgctcaa gcaaaacgaa
     1561 ataaagagaa tgcagacgag caaatcacag ctatgcaaac actttccgct gatatcaaag
     1621 caattgatgc ggaattggca gatgtggatg ctaatttaca atcaatggtg accgtacttc
     1681 ctaatacacc agctgatgat gtgccgcttg gagcagacga ggatgaaaat gttgaagttc
     1741 gtcgctgggg aactcctcgt gaatttgatt ttgaggccaa agctcactgg gatttagggg
     1801 aaagccttgg aattctcgat tgggaacgtg gtgcaaaagt tactggttct cgcttcctct
     1861 tctacaaagg tctaggtgct cgtttagagc gtgctatcta cagcttcatg ctggatgaac
     1921 acgctaagga agggtacact gaagtgatcc ctccttatat ggttaaccac gattcaatgt
     1981 tcggtacggg acaatatcca aagttcaagg aagatacttt cgaattagca gatagcccat
     2041 tcgtacttat cccaacagct gaagttcctt taacaaacta ttatcgtgat gaaattatcg
     2101 atgggaaaga attacctata tactttactg ccatgagtcc atcattccgt tcagaagcag
     2161 gctctgcagg acgtgacaca cgtggattaa ttcgccttca tcaattccat aaagtagaaa
     2221 tggtgaaatt tgccaagcct gaagaatcgt accaagaatt agaaaaaatg acagcaaatg
     2281 ctgaaaatat tcttcaaaaa cttaatctcc cataccgtgt cattaccttg tgtacaggag
     2341 atatgggatt ctcagcagct aaaacgtatg acttagaggt atggattccc gctcaaaata
     2401 cttatcgaga aatttcttca tgttctaata ctgaagattt ccaagcacgt cgtgctcaaa
     2461 ttcgttaccg tgacgaagtt gatggcaaag ttaggttgct tcatacttta aatggttcag
     2521 gacttgcagt tggacgtact gtagcagcaa tcctcgaaaa ttatcaaaat gaagatggtt
     2581 ctgtgactat cccagaagtt cttcgtccat atatgggtaa tatcgatatt attaaaccaa
     2641 attaggcact ctaaaatgag ttggtatacc aactcatttt ttccatgaac ttagctaata
     2701 gtttgacaaa cttggtagta aggtagcact ttctcaaatc cttctaacaa ccgttgcaga
     2761 atattttcta agctctggtt ttcagtcaaa agaacatcat attttaccaa tactttcctt
     2821 accttccctg acttaatttc ttgcattaaa cgttgacgat tttcctcatt gccttcctct
     2881 cgatgagttt caccttgcct ttggaccata aaatacaatg gagaggcaat cggaatcgat
     2941 aacactctag cctgcttctc gagtgtctta tcagattttt gcctctctac aaagcttacc
     3001 tcaagactaa ccccaaaact atcttttaca ccataaaccc ttagcgcaac agccacatca
     3061 tctaattggt ctgtatcctt tcgataataa acccaaaaat gtggacgccc tacttgagct
     3121 tgattagccc aattactcac tctttgcatt ttaaagccat ccatacgttg ttctaaggct
     3181 cttgataact ctgtaaagct attcctagca agttggccct ttgccttaaa agtttgcata
     3241 tcgtccttta aagggcctgc cttctcaggc ataatatact tcattgattg gaattctaaa
     3301 taatcattta attcttgata cattcttttt ctccatgtaa acaatagtta agttttcacg
     3361 attatatatc tatacatact ttcaagttct tttaaagtat gtatagcgaa gtctcgttgc
     3421 tagaaacaaa gtctaatcgc aattataact aaccaagttt tactttcttc atcacttaat
     3481 agaactcttt aaaaaccaat cttttcatag ccttctaaaa tctaacaata gctatttttt
     3541 ctagaaaatt tctcttcaat aacagataga gtctcaagtt aaggagagcc taaataatgg
     3601 tacttagaat aaagtgttcg aaatgactct ttctgaaaat tttggattag actacaatct
     3661 aaaaattggc taatgctagt gtcattgaca tatctccttt ttatcaatta tagcaaaata
     3721 tgaaactata actatgataa taccttgaaa aactaaatat aggctgagta aataactcag
     3781 cctttttcta ttaatatttt ctaaaacgtt ggtaacgttg ctccaataaa tcctcaagag
     3841 ataattgtga gagcatttct aactctgaaa ttaaacttgt cttaatcata tcgacaatct
     3901 cacttgaaaa ataaccatgt tcagggatta ctttgtctac aacttccata tggtaaagtt
     3961 ctccagcagt catcttcatt aattgtgctg cttctgttgt tcttgtacca tctttccata
     4021 aaatagaggc aaagccctca ggacttaaaa tagaataaac agtatgttcc aacatccaaa
     4081 ccttatcggc aacagctaaa gctaatgctc ctccagaacc cccttcacca ataataatag
     4141 ctataatagg gacttttaaa tcactcattt ctaagaggtt tctagcaatt gcttcaccct
     4201 gacctcgctc ctcagcacct accccaggat atgctccagc agtattgatg aaggttataa
     4261 caggtcttcc aaacttctca gcttgtttca ttaaacgtaa agccttacga taaccttcag
     4321 gatgtggttg accaaaatga cggtctaaat tatcttgaag gttcttacct ttttgaatac
     4381 cgacaatcgt cacaggacgc cctgccaagt accccaaacc tcctataatt gatttgtcat
     4441 ctgcgaactg gcgatcacca tgaagttcca taaaattatc aaaaatcaat tcagcgtagt
     4501 caagagcagt tagcctccct tgatcacgcg catcttttaa aattcttgtc acatcattat
     4561 tcatgaccaa cctcatttcc atggaaagca attagttgag caatgcagtc gcgtaattct
     4621 gttcgattta taatagcatc aacaaaacca tgttctaaca aaaactctgc cttttgaaag
     4681 ccttcaggta aatcttctct caccgttgtt tcgataacac gacgaccagc aaaaccaacc
     4741 aacgcttgcg gctcagccag aataatatca ccttccatag caaaactcgc agtaacgccg
     4801 ccggttgttg ggtctgttaa aattgttaaa taaaacaatc cctggttaga atggcgttta
     4861 actgctgccg atacttttgc catttgcatc aaactcataa taccttcttg cattctggcg
     4921 ccccctgacg ctgtaaaaat aacaataggt aatttctttt cagttgctag ctcaaataat
     4981 cttgtcaact tttcgcctac aaccgttccc atagacgcca ttataaaatg agaatccata
     5041 atagctaagg ccgtagtttg tccttttatt ttagctaaac ctgtcaccac cgcttcatct
     5101 aagttagtct tttgacgtgt agcagccagt ttttcacggt agtttggaaa attcaaagga
     5161 tccttagttt caatccctgt aaataattcc tcaaaactat cttcgtcaac tgttagcaac
     5221 agacgttctt gcgctgaaat tctaaaattg taagaacaag ctggacaaat ctttgctaat
     5281 cctaaatctt tctgataaat catatgttta caagaaggac acttagcaaa gagttcatca
     5341 ggaacctcag gaagactacg tttatcagaa gaccctaatg ctttgtttgg actaatacgg
     5401 atatatttat cctttttact aaataaagcc atcttccctc ctattgattg ttaccataat
     5461 accttggtaa aaagtcttcc atcaggaatg aagtatcata atctccagcc aagacttttt
     5521 tatcagaaat gagatccatt tgaaattctg tatttgttat aataccatca atttcaagct
     5581 catataaggc acgttgcatt ttcataagtg catcaaaacg attttctccg tgaacaatta
     5641 ctttcgcaat catactatca taataaggag gaatagtgta gcctgtgtaa accgctgaat
     5701 cgacacgtaa gcccataccg ccagcaggta gatgtaaccc attaatggta cctggacatg
     5761 gcgcaaaatt aaattgtgga ttctctgcgt taatacgaca ttcaatcgca tgaccagtta
     5821 acttaatatc attttgagaa actgataaag gaattccagc agcgatacga atctgctctt
     5881 taacgatgtc aacacccgtt acaaattctg taacagggtg ttctacttga acacgagtat
     5941 tcatttccat gaaataaaac tgtccgctat tctcatctaa taaaaactca attgttcctg
     6001 cattttcata agatacagcc tcagctgcac gaacagctgc ttcaccaatt tgttgacgca
     6061 gagtgttacc tattgccaca gacggacttt cttccaatac cttctgattg ttacgctgca
     6121 gagaacaatc acgttctcct aggtgaacta tcttaccaaa gctatctcca agtatttgta
     6181 cttcaatatg tctagctggg tatataactt tttcaatata catagcacca ttaccgaacg
     6241 cagccaaagc ctcctgtgaa gcagactcaa aagcaggctt caattcatct gctgatttga
     6301 ccttcctaat tccttttcca ccgccaccgg ctgaggcttt taacatcaat ggatagccga
     6361 tttcctcagc aattgataca gcttcttcta cacttgtaac ttgaccatct gatcctggga
     6421 taaccggaac atcagctttt atcatctctg cacgagcatt gattttatca cccattttat
     6481 ccataacttc tccagatgga ccaatgaatt ttaagttcat ctcttcacac atagtagcaa
     6541 atttagaatt ttcactcaaa aacccaaaac ctggatgaac agcttccgct ccagtcacaa
     6601 tagcagcaga taatatagca ttaacgttta aataagattc tgctgatttg gctggtccaa
     6661 cacaaatagc ttcatctgct aaaattgtgt gcaatgattc cttgtcagcc tccgaataga
     6721 tagcaactgt cgaaattccc atttcacggg ctgcacgaat gatacgtact gcaatttctc
     6781 cgcgattagc aatcaatatt ttcttaaaca tttttgatgc ttctctccct ctaaattcaa
     6841 tactatcttc aattacctat tgcaaatgtt aacgttccac tggcagctaa tttcccatca
     6901 acttctgcta ttgcttcaac aacggctata gttcctctac gtttgacaaa cttagcagtc
     6961 ataactaatt gatctcctgg aacaacttgc tttttaaatt tcactttatc catacctgca
     7021 taaaaaacaa gctttccttt gttttcttct ttagataatt ctaaaacacc tgcagtttga
     7081 gccaaagctt ccataattaa aacgcctggc attacaggat attctggaaa atgaccattg
     7141 aaaaaaggct cattgatact aacatttttg atagcgacta tctcatcttc tgatacctct
     7201 aaaacacgat ccactaataa cattggatag cggtgaggta aagcctcacg aatttctttt
     7261 atatcaatca tttgattcgt accaatcctt taccaaattc aataacttct tcattggcta
     7321 caagaatttc agtaactaca ccatcatgag gggcaggcac ttcattcatc accttcattg
     7381 cctcaataat catcaaagtt tgtcccttct tcacgctatc acctactgaa acaaagttag
     7441 gtttatctgg tccagaagct aaataagcaa ccccaactaa aggactttcg acaacgtcac
     7501 cttctgcaac agtcacagtc gactctggga tttctgaaac tacttcagta gcttgtttcg
     7561 tttgctcacc tgcttcatta gatacaggag ttgctatagt tggagtcgct tcaggttgat
     7621 gactcatagg agacatagtt ggtacaagtg gagcttttcc ctcattttta ctaaaagaca
     7681 gctcaccgtc acttgttttg aatgaaaatt ctctcagact tgactcatca aattgagtca
     7741 ttaaatcctt aatctcttga atatccaatt taatcctccc agcgtttgaa ggccagaact
     7801 gcattgtgtc ctccaaaacc aaatgtattt gaaatggcat aacgaatatc agcgtcttgc
     7861 ccttgaccaa aaataacatt cgctgtaata tcttctgata attctgttgt tccagcagtc
     7921 attggaacat aactatgacg tatcgcttca atagttgcaa ttgcctcaac agctcctgcg
     7981 gcacccaaaa gatgaccagt aaaagattta gtcgaagaaa ctggaacgtc tgtcccaagt
     8041 gcagcaacaa cagcttgact ctctcctttt tcgttagcag gtgttgaagt tccatgagca
     8101 tttacatagt taacttcttc aggtttaata ttagcttcag ccagtgcaag ttgaatagct
     8161 ttagtggctc ctaaaccttc tggatgtggt gaagtcatat gatatgcgtc acaagtgtta
     8221 ccataaccaa caacttctgc aagaatagta gctcctcgtt tttcagcgtg ttctaaactt
     8281 tctaagacca acattccaga accttctccc atgataaatc cattgcgatc tttatcaaat
     8341 ggaatagatg ctcttgatgg atcttcagtt gttgataatg ctgtaagact ttggaatcca
     8401 gcaatggcaa attttgtaat agcagcttct gcaccaccga ctaccataat atcttgaata
     8461 ccaaatttta tattacggaa agcatctcca atagcatcat tagaagaagc acaagctgta
     8521 ttaattgatt tacatacacc atgagcgcct aacctcatcg caacatttcc agctgccata
     8581 tttggtagag cttttggaag cgtcattggt tttacgcgtt ttggcccttt ttcatgaaga
     8641 cgaatcactt gttcttcaat ctcttgaatc cctccaatac ctgatgcaac gatgacacca
     8701 aatcgatctg catcaatttc atctaaattt aaattagcat gttgaatagc ttcacttgaa
     8761 gcatataagg cataaaggga atacatatca aatctgttca gatctttttt gacaaaatat
     8821 ttatcaaatg gaaaatcatg aatttcagca gcatttttga ccatgaaatc acttgaatca
     8881 aatttagtga taggtccaat accaacattt ccttctttaa ggctattcca aaattcttct
     8941 ggtgtgtttc caataggtga ggttacaccg taacctgtta ctactactcg ctgtaatgtc
     9001 atattatttc ctctttatta ttgcatcgtc ataccgccat caatggcaat aacttgtcct
     9061 gtaaggtatt cttgttctgc taagaaagac gctacctgcg ctacttcttt accttttcca
     9121 atacgtttca ttggaatttg agctaaaata gcttcttgca ttttttctgg gataacatct
     9181 gtcatatcag attcgataaa gcctggagca atggcattga cacgaatgcc tcgtgctgct
     9241 acttctctgg caactgattt tgtaaagcca attaaacctg ctttagaggc tgcgtaatta
     9301 gcttgcccta catttccagt taatccaacg acgctagaga tattaataat agcaccttga
     9361 cgcgcttttg tcatcggttt caagactgat tgtgtcatat taaatgcacc tgttaaatta
     9421 attttaagaa cgctctcaaa atcttcaaca gtcattttaa gcatcaattt atcattagta
     9481 ataccagcgt tgttaacaag cacatccacg ctacctagac ttgcaatagc ttctttaatc
     9541 atgcgattag catcttcaaa agaagaaaca tctcctgaaa tagcaataac ttttacacca
     9601 taatcagcaa attcagctat taagtcttct gatatttcag aacgtccatt taaaacaata
     9661 ttagcaccta attgtgcaaa ttgatgggca attgcaaggc cgattcctct acttgaaccc
     9721 gtaataaaaa tgttcttatc ttttaattgc atattttccc ctagtttaat tccttaagat
     9781 tattaaatcc aattttatct tcaacagaat atactgataa tgagctatct atttttttca
     9841 agaaacctga gagtaccttc cctggaccaa tttcaacaac ctgtgtcatt cccatttttt
     9901 tcatggtttc aatagactca tcaaagcgta caggttccat aacttggcgt gccaataaag
     9961 atttgatatc gtcttttttc attatctgcg cctctgtatt tccaattaca gggatttcag
    10021 aaactgaaaa atgaacttta tctaaaacat cgctcaattt ctgacttgca ggtttcaata
    10081 aagcagtgtg aaaaggacct gaaacattca gaggaattaa acgttttact ccttgttgtt
    10141 ttagttcttc tacagctgca tttacagcga ttgactctcc ccctataaca atttgctttg
    10201 gagtattata atttgcagga ctaacaatac caaactgaga agccttctta caagcatctt
    10261 caattatttg acgatcagca tttaaaaccg ctaccatttt gccactacca gcaggtgctg
    10321 ctgcttccat tagttggcct cgtctagaaa caagtactac agcatcttca aatcttatag
    10381 cacctgaagc aactaaagca ctatattctc ccaaagaaag tcctgcaacc atatctggtc
    10441 taagtccaat ttctttaaga attaaacgat aaatagcagt cgaagtcgtt aaaatagctg
    10501 gttgcgtata tttcgtctga tttaatttgt ctaagtcttt atcaatcaat tctctcaaat
    10561 catatcctaa aacatgactt gccttatcaa aagtttcttt aacaatcggg aaagtttcat
    10621 ataaatctct agccattcct aacttttgag caccttgacc agcaaataga aatgcaactt
    10681 tattcatagt gtctccttat ttgtatttat aaatctgacc agcggcttgc ctcagaaaga
    10741 ataacttgac gcgctcctga ataaatatct tcaagaatct cttgacaagt ctcttctgat
    10801 ttaattaatc ctgcaatttg tcctgccata actgaaccat taataacatc cccatcaaca
    10861 actgcattgc gaagagctcc cgctcctaat tcttcaatct cattgattga aatacggcca
    10921 gctaaataat ctttttctgc ttggctatag gttgttacta gtttattttt taaggcacga
    10981 actggatgac ctactacctg agcagaaaca gcagtgtcga tatcttttgc ttttaaaatt
    11041 tttgctttgt aattagggtg agcattagat tctttagcaa caacaaaacg agttcctacc
    11101 tgaacagcat cagctcctaa catgaaaccc gcagcagccc cacgcccatc tgctatacca
    11161 cctgcggcaa tgactggaat tgtaacagca tcaacaacct gtcgcactaa tgtcatcgtg
    11221 gtcagcttcc cgatgtgacc acccgcttcc attccttctg taatgatagc atcagcccct
    11281 aatttctcca tacgttttgc taatgcaaca ctaggaacaa cagggattac tgttatacct
    11341 gcttcatgaa agcgttccat gtacttacca ggattacctg caccagtcgt tacaactttt
    11401 acaccttctt caataaccaa atcaacaata tcatctacaa aaggtgataa aagcataata
    11461 ttcacaccaa agggtttatc agtcatagat tttattttat caatatttgc tttgacaacc
    11521 tctttagggg catttcctcc accaataata cctagaccac ctgccttaga aacagctcct
    11581 gcaagatctc catcagcgac ccacgccata cccccttgaa aaattggata ttttatattt
    11641 agtaattctg ttatacgtgt tttcattttt ctttcctaaa attaataatt tgacaatcaa
    11701 actatattga taagaagaag agtgagtacc gatcaacgat tgattgaata ctcactctaa
    11761 taagtgattt ggtaagaata tcttttatta tcacttgctt ctttttttct tacgacttaa
    11821 ttattttact ttttcttcaa cataagcaac taaatcacca acagtattta gtccttcttc
    11881 tgtctcaatt tggatatcaa atgcatcttc aatttctgaa atcacttgga aaacatcaag
    11941 cgaatcagca tcaagatcgt caaaagtagt gttcaacgtt acttcttctg catctttacc
    12001 aagttcttca acaataattt cttgtacttt ttcaaatact gccattttta gctccttaaa
    12061 ttttaaataa tcttttataa atgtgctatg cactatatat aactaaattt taacaattag
    12121 acttccccat gtcaaacctc caccgaaacc cgataagagg attgtttggt taccatctag
    12181 tttcaacaaa ccgttctcat aagattctga aagtaaaata gggatactag ctgcactagt
    12241 attaccataa tccatcatat tctctggaaa tttatcacgt gctatatcaa tttttcgaga
    12301 catcttatct aaaatccttc gattcgcttg gtgtaagaaa agataatcaa tatcttcttt
    12361 ttccaaatgg gatgtttcta taagatgatt gatacttttt gaaacttctt tgatagcaaa
    12421 atcaaagatt gctctaccat ccattttcaa aaatttatca tcaagtactt catctgagaa
    12481 aggtgaattt aacccgactt gactagattg aagtccctga cgactaccat ctgtattgag
    12541 agactctgca agaaaatgtt tttccttaga agcttctagc aagacacctc cagctccatc
    12601 tccaaataaa actgctgtcc ctctatcagt ccagtctaat actttagaaa cagtttctgc
    12661 tcctattact aaaccttttt gatagctacc tgatgatatc aatttttcgg cggtagacaa
    12721 ggcaaaaaca aaaccactac atgctgcact caagtcaaag gcaaaagcat tactcgctcc
    12781 aatatgagct tgcactcttg cggcagtaga aggcatcata gagtctggtg tcatcgttgc
    12841 aacaataata aagtcaattt gactagcgga ataaccggat ttttcaataa gttgttctgc
    12901 gaccttattt gccaaatcag ctgttgtttc atttttacta atatgacgtt gttttatacc
    12961 agtacgtgac gaaatccagt catcactggt atccatgatt aatgataaat cctcattttt
    13021 aatgatttga gaaggtgcat aatgtgcaag ttgactaatt ttagcaaaaa ccactagact
    13081 aagtcctcca aaaatttatg aagattccct aaagcgctcg tcaacatttt tatgtcttct
    13141 tcgcctaggt cttcaataat atgagatacc atagatttat gaaatttact atgcaatcga
    13201 tctaataacc tacctctctt agtaagactc aagtgaacaa ctcgacgatc aatacttgac
    13261 cgtttacgct caatgtagcc ttttttttct aacttattaa ggctggttgt tacagttccc
    13321 aaagttaaca ttaattctcg agcgacatcg ctaggagtta cctcagaatg tttcccaata
    13381 atttcaatag tatgcatttc ttttaaagat acatcgctaa attggctagt ctttaaactc
    13441 atttcctcaa taatcatgat tctattaaaa atgtcaacta aataactatt aatctgatcg
    13501 tattccaaga tatcaccacc tcacattaaa ctttgatagt caaattatat caaagttcaa
    13561 acttatttgc aagactttaa tcttaaataa tttgattttc aaactatttg cctaaaaaat
    13621 taggacgcct tctatcagcg tgtgcccgaa caccctcttt aaaatcttgc ttaaaggcta
    13681 atgattcttg taactgtaat tcaagctttt tatattgttc ccaatcttta aaagcagctt
    13741 cccaagctaa tgattttatg gcttgatatg agtttataga atgtctactt aaacgcttta
    13801 atagttgatc cttaatttta cctattttat ctgattcaca aagtttataa acaattccca
    13861 aagcttcagc tttttcagca gataagcttt ctccagttaa agctagttgc actgcacgag
    13921 ttataccgat tgatttacta agtaacaata aacctcctgc atctggagct aaaccgacac
    13981 ccacaaaagc ctggataaat ttactcttct tgcttgcgat aacaaaatca gctgctaatg
    14041 caatattagc tgcggcacca gctacagccc catctacaca cataacaaca ggtttctcta
    14101 gctgtagtag atcatacgat atctgattaa ctaactcagc aattttagtt aatgagctaa
    14161 tatcatcgct ctcaacagca tctttcatca cttttaaatc tcctccgatt gaaaagattg
    14221 gtccttgtgc ttcaatcact aaaaacatga catctttatt ttcagacata agccttattg
    14281 catcaataat ttcttgacac attgggatgt taaaaccatt tgaaatatct ggtcgattaa
    14341 gcataatagt ggccacgctg ccatcaatac catatattat attttcgaac ttcaaaacgt
    14401 ctcctctctt taagagtgaa agaaaaataa aaaataattt gattatcaaa ttatcatgct
    14461 ttgtatttta tcataaacct agctttcatg gcaagataaa aaataaaaaa aaacggaatt
    14521 aaacctcccg tttttaatag ttatctaatt tcacaaaaaa ttgactcaga tcgctcacta
    14581 atacattagc ctgcgactga ttcattccaa agtatttgtc ttcaatagcc cacacatcaa
    14641 taccagctgc aatgccagct gcaatcccct tctcactgtc ttctacaata agaatttgct
    14701 gtttaggaat atctaaaagt tctgctgctc tattataaat agctggatta ggtttacttt
    14761 ctgaaaattc ctcaccagac agaattacct taaaatatgt atccagatta aaactttcta
    14821 atgctaacat gatatcatgt ctagtagaac ttgaagctag acccaaaaga tagcctttgt
    14881 gatgaagctt ttcaatgact cttttacaat cctgaaaaat taaatctttg tatggcaagg
    14941 gattatcctc tttatagcga ctataatctt gttggagctt atcaatatcc caggtatcat
    15001 attgatcacc taaaacagat ttccaaacct gcttcatatt tcctccaata aaaaaattca
    15061 ttggtaaatg gttaattgta attccatgtt gttttaaaaa acgctctcgc cttt
//
LOCUS       DATRME010000047        15057 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRME010000047 DATRME010000000
VERSION     DATRME010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15057
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(75..1502)
                     /locus_tag="VBC23_001408"
     CDS             complement(75..1502)
                     /locus_tag="VBC23_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6843351.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(1519..3387)
                     /locus_tag="VBC23_001409"
     CDS             complement(1519..3387)
                     /locus_tag="VBC23_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6843352.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(3380..4231)
                     /locus_tag="VBC23_001410"
     CDS             complement(3380..4231)
                     /locus_tag="VBC23_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6843353.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(4569..5177)
                     /locus_tag="VBC23_001411"
     CDS             complement(4569..5177)
                     /locus_tag="VBC23_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6843354.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(5257..6294)
                     /locus_tag="VBC23_001412"
     CDS             complement(5257..6294)
                     /locus_tag="VBC23_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6843355.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(6348..7076)
                     /locus_tag="VBC23_001413"
     CDS             complement(6348..7076)
                     /locus_tag="VBC23_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843356.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            7252..8244
                     /locus_tag="VBC23_001414"
     CDS             7252..8244
                     /locus_tag="VBC23_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6843357.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(8303..9247)
                     /locus_tag="VBC23_001415"
     CDS             complement(8303..9247)
                     /locus_tag="VBC23_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6843358.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(9258..10067)
                     /locus_tag="VBC23_001416"
     CDS             complement(9258..10067)
                     /locus_tag="VBC23_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6843359.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVIDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(10193..12430)
                     /locus_tag="VBC23_001417"
     CDS             complement(10193..12430)
                     /locus_tag="VBC23_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6843360.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPHLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            complement(12414..13067)
                     /locus_tag="VBC23_001418"
     CDS             complement(12414..13067)
                     /locus_tag="VBC23_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6843361.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
     gene            complement(13167..13907)
                     /locus_tag="VBC23_001419"
     CDS             complement(13167..13907)
                     /locus_tag="VBC23_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6843362.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            14042..14806
                     /locus_tag="VBC23_001420"
     CDS             14042..14806
                     /locus_tag="VBC23_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6843363.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMHQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            14799..>15057
                     /locus_tag="VBC23_001421"
     CDS             14799..>15057
                     /locus_tag="VBC23_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6843364.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKN"
BASE COUNT         5035 a         2882 c         2220 g         4920 t
ORIGIN      
        1 ttataataaa aaggagtaac ctatggatac tcctttttat attaaattga aacagagact
       61 agttgtttaa catgttatct gttatgatgt tcaaacaatg attggccatt tgtcttaatc
      121 actttttggt accaaccaaa tgacttcttc ttgtaacgtt gtagactccc tgttccatca
      181 tcatttcgat caacatatat tagaccataa cgtttactta gttgagctgt agacatggat
      241 acacaatcaa tgcatcccca agatgtatat cccattattt caacaccatc ttcaatagct
      301 ttagcaactt gaactaaatg ttgattcata taatcaatac gataatcatc ttcgacagta
      361 taatcaccgt ctagtgtttc tatcaattga tcttttgctc ccaatccatt ttctacaata
      421 aacaacggaa tctgataacg ttcataatac tgatttaaga ctaggcgtag ccctatggga
      481 tcgatttgcc atacccattc tgaagttgtt aaataaggat tagtcaggcc tccaaggata
      541 tttccctgac cactttgata attttcaaag tcataagctt gtgtcacgct catatagtaa
      601 ctaaacgata ggaaatctac tgtattttgt gccaatactt cctcatctcc ttcttcaaat
      661 ttaatttcaa taccattatt tttgaaatag gactgaatat aagtgggata tttacctctg
      721 acatgtatat ctgaaaatag cagattatgt tgttcaaatt gtcttgcagc cagaacatct
      781 cttggatcag atgtcatagg ataagctggc atagccagaa tcatacagcc aattttaaaa
      841 ttagggttga tagaacgccc taattttgtc actctagccg atgctactaa ttcatgatga
      901 atagcttgat aaagctcctg aggacttagt tgtgatttgt cggccataat agcaccactg
      961 gtgaaaggca tatgtaaaat agaattcact tcattaaaag ttaaccaata ttttacctta
     1021 tctttataac gctccatgac tgtttgagca aatttctcaa aaaatgctat caaacgtcta
     1081 tcagcccaac cattataggt ctttgcaaga tgaaggggtg tttcataatg agataaggta
     1141 accaaaggct cgatattata tttaagcaac tcgtcaaata aattatcata aaattgtaag
     1201 ccagcttcgt ttggagcact atcatcacca tttggaaaaa ttcgagacca ggcaatagag
     1261 gttctaaaaa ctttaaagcc catctctgca aacagtttta tatcattttt atagttatga
     1321 taaaaatcaa ttgcttctag cttcaaattg tctggagtag gtttggcggt aaaatcgcct
     1381 agaccaccat ttggaagtac atcttgtaca gataaacctt tgccatctgt tctaaatgca
     1441 ccctctacct gattggcagc aactgcacct ccccatagaa aatgtttagg aaaaatcgtc
     1501 atattatcat ccttcctttt aaagaattac aagtaaatca tcttcatttg taaccattgt
     1561 ctcttcgtct ttaacgataa tttcaagttg gtcaattgaa tttgtaacta caactggact
     1621 aataagagaa tacccctttg aactaataaa atcaatatcc attcgcaaaa gtaactgtcc
     1681 tcgtttaaca aaatcgccaa ctttaaccaa ttgttcaaaa ccttctccat ttagttctac
     1741 agtatctatt ccaatatgga ttaacacttc aactccctta tcatccttta aggcaatggc
     1801 atgttttgta ggaaataaac ttataatttc accatcaaat ggtgcataaa cttcacccac
     1861 actaggagtg attgctaaac cttcacctag taatttagat gaaaatgttt catcgggtac
     1921 tttagataaa ggtaatacat tgcctaccat aggtgaatac aatgtctctt tttttgataa
     1981 accacttcta atatgtgtgt actcttgagc attaatacta cttttttcgt tttctccttc
     2041 atcaattccc aagaaccatg taataatgaa agtcaaaata atagtagcta ttgctacaat
     2101 aacagcatta ctgaaattat tgcccccctg tggattgatg tattgtggca aaccaataat
     2161 agatggcact acaaatgtat aggaagcaat attaactaat ccaatataag ctcctactaa
     2221 accacctgat atcattgcag cgtataaagg cttcttgaat ttcagtgtga caccataaag
     2281 tgcaggttct gtgataccag ctagtagtgc tgataaacct gctgcaaagg ccacctgacg
     2341 tgtctgtttt tgtttagcct taactgcaac agcaagggaa gctgcacctt gagctaaatt
     2401 agaagctaac attgctggta aaatcaaaac atctggtgtc gtaacagaag cagctaaaaa
     2461 gattggagca aatgcccagt gcattcctgt cataacaacc aatggcataa tagcaccaag
     2521 aatagctaaa gctaaccatg gtgccacatg gtaaatcgaa agcattgcac tagacaatcc
     2581 tttaccaata attactccca gtggccccac gaccactaag gctaaaaagc cagaaatgag
     2641 aataatcaaa gtcggttgta aaaagctctt cattacacta ggaacaattt tattaatcca
     2701 tctctcaatg tattgcataa gaaaaaccat aattaagatt ggaataacag aagaaccata
     2761 actagccagt gtaaccggag ctccaaagaa agataatggt tttccttcag ctaccattgt
     2821 cacaaaagca gggtgtaaga gaatccctcc tactgtagca gctaatacag gtgtaacttt
     2881 aaatcgtgac gcagctgaat aagcaatcat tattggcata aaataataag cggcatcacc
     2941 aaaaaaattg ataaaagcta ctgtttgaga actagagctt gttataccga gcatagggag
     3001 caaaatacct ataaccttga gcatacctcc tcctagaaga gcaggtataa gcggcttaat
     3061 gactcctgca atcgtttcaa ttaatttctc aaatattgaa ctcttttttt gtgatgaaaa
     3121 ttctctggta gtattttcaa aatgacctaa ggccaaaaaa gcattataat aattattgac
     3181 atcatttccc aaaataattt ggtattgatc atttttacgc ataacaccga taacattcgg
     3241 aatagtttta ataacctgat cgttgacaat ttcatcattg tctaaaacta atcgtaatcg
     3301 tgtcacacag tgggttacgt gttgaatatt tttttctccc cctactgctg ctaaaatagc
     3361 tttagcagtc tcttggtatt tagtcatctg atatctcctt taaattttta actaatcgtt
     3421 taacatgcac cgtcagatat aggagttcac ttgaattaag ttgatattgg tattggatag
     3481 ccatattgtt gccgatctct ttaacacatt tatattctct cggatatttt ttcttaacta
     3541 ccaaaagtaa atcagcatca tcatcttcgt atttcattcc tgataaaacg cgttgagcaa
     3601 ataatttggt atgtgtcatg aaacgataat aatcaattga actttcatct agttctgttc
     3661 gaaaatctat ttttactttc tgttcaatgt aagatacaat ctcagtgatt ttatgcgctt
     3721 cattaaacgg attatccaat ccagcattta caaagtgcat agcaataaaa gctgactcat
     3781 caattgttaa acaaataccc aattcatcct ttataagttc cagagccttc atcccaatgg
     3841 agtattcatc aggatagtaa cgctggattt ccaaccttag tggattttgg ataaccattc
     3901 tttgttcatg tctttcaata gccgaatgaa tatgatctgt caaattgata taaagaattt
     3961 catctaagtt cttacccaat ttgattttac ctaggttgat aattcgttct gaacatgcaa
     4021 ctacttcttc tggaacagta ataaataact ccgtaaaacg gttcatgtta tcactatttt
     4081 ttaaaacaaa tgacttctca atagcatcgc tattaatcct atcacctatt cttttcttaa
     4141 atgcaattcc tttccccatc agtaacacat ctaatccttg atgtgttact gatatgactg
     4201 cattatgatt taatacgcgt tttataatca ttaaagtcct agtccttcac tcgcacaata
     4261 aaaaatgacc catccaagac acaacaaaaa gtcgtcgttg aataggtccc tgctcacaaa
     4321 atgtgataac atcctttatt atgcaatttt atctgttatc agcttgctta ccttaaataa
     4381 taacacctga aaacgtctta attttatcat ttcttactaa tcttgtcaag cgctttcaat
     4441 aaacttattc gcttaattga agataatagg actgttctag ttctatctga gtttggattt
     4501 ctagactttt aaaaacatta tcaactttta gaataataaa aaaacgagcc tatcgactcg
     4561 tttttttatt aagctttagc agcttggtaa agttcattta ctttattcca gttaataatt
     4621 tcaaagaaag ctttgatgta gttaggacga acattacgat agtttaagta ataagcatgc
     4681 tcccatacat caagtcctaa aataggtttc ttaccttcca taattggagt atcttgattg
     4741 gcagttgaaa gcacttcaag tttgccttca gcattaacaa caagccaagc ccaacctgaa
     4801 ccaaaacgtc ctgttgctgc tgctgtgaaa gcagctttaa agtcttcaaa tgaaccaaaa
     4861 gttgcatcaa tgtcttcaga taactcttgt gaaatttgag tttcttctgg tgacatcaat
     4921 tcccagaaaa gagcgtggtt aagatgtcca ccaccgttat tgatgactgc ctgacgaata
     4981 tcttctggaa tttgagaaac atcagctaag agcgcctcta agtcttctcc aatttcagga
     5041 tgtttctcaa gagcagcatt tgcattagca acataagttg catggtgctt atcatgatgt
     5101 agtgtcattg tctcagcatc aatatgtggc tcaagcgcat catatgcata gggtaaatct
     5161 ggtaaaataa ttgccatgtc gaaatcctct tttcatctat tgttaccctt attctatagg
     5221 aatttggaac agctttcaac tcttttgttt ctttaattag ggctttttat gagcaatttt
     5281 taataaggct aaatcaaaga gataatcctt atcataagtc cctcttttaa tagcatagtc
     5341 agtctcgatt aaaatcctaa tcgcttcttt taaaaaagca aggggaaggt ttctagaatc
     5401 tctgacagca aattttactt gataagggtt gattttacgt cctatgtaat gggatagttc
     5461 agaaacaatt tgactctctg actttccttt actagccaat attttaactt gcaaaaacat
     5521 ccgaaactgt cctaacatga ttgcaatcaa tttgatttcg tcttcgcctt gtagacgtaa
     5581 atctcttact aaatcacgcg ctaaatctat cctaccaagt aagacatctt gagtcaaatc
     5641 aaatatatta tcttgcaggc ttttagggat agcctctcta acatcattag acgaaatatg
     5701 accgtctgtt ttatatgact ttaagaaagc gatattagtt aaagtatcac taaaatcata
     5761 gttagactta atcaataact catcaaaaac acctgcttca aaaactaatc cttcttggtg
     5821 agcatatttt tgaaaatacg tctttaaatc tgattctttt aaagtattag cctctaaaac
     5881 acgagcatca cgtttcaata actttacaag tcgacgtttt ccatcaagtt ttcctggagc
     5941 acaaataaca agtctcgtag tatctacagg attctgcaag taggcttcca agcgcttcat
     6001 tgcttgttca tctaaataag tttttttatc agttgtaata tcttgaaact gatcaaaaat
     6061 aacaaccttg tagtctgata aaaaaggtaa actttctaag tctaattccg cattttgata
     6121 ttcttcttca cttaaatcaa aataagaata ggctaaatcg tctttattga atccaattac
     6181 ctgaaatagc ttctctttca tctgagcata ttgccctaaa tcttctcctg ctaaaacagt
     6241 tactaaccct aaattatctg gggtaattct cccaatttct tcaatcgcaa tcatattcct
     6301 tattatacca taaggaaact tttcaaggtg tcaaataata cttaaaatca ttcatcaact
     6361 ctatgagaaa taattccaaa ttgacaatct cggataaaat ggtcattatt ctcttctaat
     6421 tctttttgta aattgacatc ttctaacccc tgaaggtcac tatatttttg tattctttct
     6481 ttcatgatat ttagtagcca agatagatct gtaccagttt gtgacgtttg aatgacattt
     6541 tctgatacaa aatggtcaac gattaagcct gcttctaaca ttaattgctc aagttgactt
     6601 ccaatatgaa tgtgtcctcc ttccttctca acagtttgcc agattagtga ttgtatttta
     6661 ttcatattta caaagctagg cttagctagt tctaaacaca ttgcatcact ttcttgaaaa
     6721 acataaaaac cattttgctt taacaaaggt gttaaacttt ctattacttt ctttggattt
     6781 ttttgataca ttaatacacg acgtccaata atagcatcaa aactttttgt ttcaaaggag
     6841 gaaagtgctg ctaaatctgc attttgataa ctaacattag catagccatt atcatgtcta
     6901 gcttgccata aaagttgctc attaatatca attcctacaa catcaccttc tttaccaacg
     6961 atatcagcag ccaaacgcgt taattcccca ctaccacaac caatatccat aactctcata
     7021 ccgggctgca aagctttttt taataatgct tttgaaaaat cttctctgtg aaacataata
     7081 acactctcct ctcttctatt ttagcaaatt aaaggttcat acgtactatc agcaaaaaat
     7141 tataataggt tgaaatttta tagcattatt ttcttgtttt cgtatctact ttattataga
     7201 ataacaacta atcattttta gcatgacgag ctcaattaca aaaggataat catgaaaaac
     7261 tatcgaaaac ttattgtact actacttcta atcttttttg ccatttttat gggagcatat
     7321 gcttacacgc atattgttga aaaaagatcc ctaactagca atactattga aaaaactcta
     7381 cctgtggtaa atcagattaa gcctcaaacc attaaagaat accaaaatta cttaactaag
     7441 gtagctaaac gtaatgttct tcctgtagac attcctcagg cattaaataa tgaaaaggta
     7501 gaaattactg ctactgatgg catgcaaaca ttcacttgga atgataaaaa taatcctaag
     7561 caaaaggtta tcttctatgt tcatggagga tcatatatcc atcaagcttc cgaattacaa
     7621 tatatttttg tcaataaact agctaaaaaa ttagatgcaa aagttgtctt tcctatttac
     7681 cctaaagctc ctacatataa ttatagtgat gctatcccca aaattaaaaa attataccaa
     7741 aatacattag ctagcgtcac atctcacaaa cagattatcc tagtaggtga aagtgcaggc
     7801 ggaggccttg ctttaggtat tgctgataac cttgtcacgg agcatatcaa acaaccaaaa
     7861 gaaattattt taatatcacc atggttagat attgctacta ataatccaaa gattgaaaaa
     7921 gttcaaaaaa aagatcccct tcttaaagct tggcaattac aacaagtagc tccttactgg
     7981 gcaaatggaa aaaagaattt taaaaatcct caagttagtc ctctttacag tagccaattt
     8041 aacaagatgg ctccaatatc attttttata ggaacgcacg atattttcta ccccgataat
     8101 caattattac atcaaaaatt agcaaaagaa aacattaaac accactatat cgttggtcaa
     8161 aaaatgaacc acgtttatcc agtcttaccg attcctgaag cagagactgc tttcaaacaa
     8221 atgcttaaaa ttatcaatca ttaaataaaa aggtttccaa tcaagtttag ttggaaacct
     8281 tttttctatt acttttgaat gactaattat cttgagataa aaactctgag aataaaactt
     8341 cttgactaat cttttcagct aaagcctgtt taaaagctaa tttccctaaa taataggatt
     8401 tgtggtcaat tgttgggagt ttcaaaagtt gcccaactaa taaacattcc tgaccaataa
     8461 tataaggaca ttttttccta ctttcttcat aaccttttat tagccctgcc gcaacttcat
     8521 cacttgtagc taatatacca tctatactaa cttcttttac caattgataa gataaattta
     8581 gtccatcatt aaagtcatga acattcccaa ctaccatata tggagaggat agttgcccat
     8641 agacctcttg ataagctaat aatgcggatt gataggtagc agaggattca ttattccttg
     8701 aaaataataa aactaagtgc tccaaacctc gtaacttcat atcagaaaat gcctctagaa
     8761 atgaagaata gcgatcaagg taagcagagg atagatgatt atattcctgc aatttttcac
     8821 aaactactat tcgtccatat tttgcataag tttcaataat gtcgaggcta attgctcttg
     8881 aagtgaatat taaagcatca atagcttcca tcttgagctg tttcaagtag gaaagttcta
     8941 attcttggtt gtaatccgaa ggcatcacaa caagttgata gtcggtagtt ttagcagcat
     9001 ctaataggcc atttattaat tgtgtaaaat aaggatggcg cgtgtgggga atgacgacac
     9061 ctattttttg tgttttgcct cgacttaaat cccgcgctag ttgatttggt atataatcca
     9121 attcggtgat aagagccatt actttttgtc gcgtctcatc agaaacatgt gggtgatgat
     9181 tgataacacg ggaaaccgtt gatttagcaa cacctgcctt tttagctata tcactaatac
     9241 ttgtcattat aaggctccta ctttaaaaat ttttgaatcc catatcccac tccgtcttca
     9301 tcattagact tagttagttg atatgctatc gctttgatat catcaaaagc attatctata
     9361 acaatagggt aacctaccat ttcaagcata ggcaaatcat tatgtccatc tccaaatgca
     9421 gccgtttctt ctctagctaa ctgctctttt tgaaggatgt aagcaattcc tttagatttt
     9481 ttggctaaga gatgtgtaat ttcaagatac gctttacctg aacgttgaat agtgatttct
     9541 ggtaactcca aactttgaag atatttttct aattctagca tatttgcttc atcgaaagta
     9601 atcatcataa ttttaaaagt atttgtgtga ccttctaaaa actgatcctc attatgaata
     9661 aacgttgggc attgttgagt taaactatgt tcgtatcgga ttccttcatc tattttatca
     9721 caataccagt tattaagatc gtaataagat aaactaactt gaggaaaatg gaaccgtatt
     9781 cctcttagca attgttttac tgtgcttttt ttaatgattt gtgtatgaat aggtagaact
     9841 tgattattat ctcctattct ataaatcaat cctccattaa aagcaacttg gactcctcct
     9901 aattgaagtg catctacggc atccttcatt tccataggtg ccctagcaga cactaaagtt
     9961 actggaatcg ctgcctcacg tattaaggta gcgttacttt tactcacttg tccttcacta
    10021 tttaatagtg taccatccat gtcacaaaaa atacgtttga tagtcataaa aaatacctct
    10081 cttcttctga aatcagtata aatgctggaa cccgttccat gtcaagctta aaattactga
    10141 atccttgcga aaaatagatt gtatttaaca gatacgcaat cattttaata aatcaacgaa
    10201 ctgtctgaat atgccacttc caccatcctg ttagcctaat tgtacctgat tgatccgttc
    10261 ggaaaatctt gcttttaatc ttttggaacc tttgtaaagt ctcttgatga ggatgctggt
    10321 aacgattatt tttaccagct gacactagaa ccacactagg agacaacttt ttcagaaagg
    10381 atagacttga tgagccctta gaaccatgat gtcctgcttt aaggatatct actgataaat
    10441 gaggataagc ttctaataac tgcttttctc cctcttcctc caaatcaccg gtaaataaga
    10501 agccttttcc taataaatgt ccatataaaa caattgaatc attatttttt ccatcaccct
    10561 tgtgccatgg gtaaagtact tgtaaataac ttcccatgac agaaagttta tcaccagctt
    10621 ctatagattt tacagctact cctatctttg ataaacgctt aacgtactgc gaattcgtta
    10681 aagaacctga acttgtaatc aaacgtgcaa ctttaaaatg ctttgagata acttccatat
    10741 cccccatatg atctgtatcg gtatgagtta tcactaaatt atctatcttg ctaatacctc
    10801 tgcttttcaa gtaaggaatg agcgttctct cagcattaga ctggtttact ttttgtttcc
    10861 attcttcagg ctgttcaaat ctcacccttc ccccagtatc aattaaaatt gtttttccta
    10921 accaatccct cactaaaata ctatctcctt gtccaacatc taaaatcgca acctcgttag
    10981 taattggatt cttagtgata aaaaacaagg taaagataat aagaagggag caataacgaa
    11041 aacatttaga atgataataa tcataaagta atcccaaaat tataatcatg actattaaaa
    11101 gaaaaaggct gggctttcca aaaataagtg gtcttggaaa cagttgccca gtccatttta
    11161 ataatacttc taggatttca aacaaactat taatacaact taatttaacg aacggtgata
    11221 agacaaatat aacagttaat aaaggcaaaa atataatatc aaatgcaaaa gataaaagtg
    11281 ctgttaatat aattgatatt ggttgaaaac ccgaaaaata gtaggttagt atgggaagaa
    11341 ttcctacaga gactgtcaaa gattcgatag ctactttttt tatactcgaa aaatgatcaa
    11401 aggaggtcat cgtaagtata aaagcataag caaagctaag aactcctccc gtagtcaaaa
    11461 gtgagtgggg gagagagata agacaaatta acaagcagag agcaaagttc tcatctttct
    11521 tgacaccgct atgtgataat aaagattgaa taagtgccct aacgactgag atactaaagc
    11581 ctgttaaacc agcataaatt agcgagaatg gtatttgaag taaccaaaca tggtctagac
    11641 gtaagccaat acgtagacag atataacgaa aaataccgag aaaaaaacct acttgcatac
    11701 ctgacaaagc aaataaatgt ataataccta gactactata taaatcggac atgtcatcaa
    11761 aggtcttatc tagatatcca aacaaaagcc ctgacatata gtgacgcata ggatttggaa
    11821 aagtttgact aataacaatt gcacttctac gccacttaaa cataagatca aagaaagacc
    11881 tatgttgaaa taagcgtatc tgttttatct ttgtcacttt ggcgatacga taaattccat
    11941 gtctttttaa atactgacga taatcaaagc cattaaaatg ccttctctcc tctgcttctt
    12001 caagtttgat atctgcaatt atcattatat tattatcaag attttgaaaa taatctttct
    12061 cacttttatt ttttaatgtg taaaaagctt gataagtgtt acctgaagca tctgcctgga
    12121 atgataaaac atcaccattg atagaaatag tatcaatacg cactttaatc tggctaatat
    12181 gattcggtac tctttgatat tggacattca tattgtgatt ttgccataaa aaataaaata
    12241 agaaaaaaat tactattgtt attgttttaa aaatagtttc acgatcatat tgataaatca
    12301 gaaaaataaa agtaaataaa agaaaggcac aaccaagcat tgtccaagaa aacactagta
    12361 agtaaatttg gaagaccaac aatgctaaat aaataggttt tagaggaaaa tacttagtca
    12421 agtgtaacat cttgacgtaa tttttctagt gttttctcac caatacctga tacatttttg
    12481 aggtcatcaa tagttttaaa accaccttga ctatcacgat aatctaaaat atcttgagct
    12541 cttttagctc caacgcctga tatggtctga agacttgata aatctgcttt attaagatta
    12601 attttcccct tactatttcc ctgagtagca atattatttg ctttctcctc ttccacaaca
    12661 gttatttttt cacctttttg agcaacatag ataaccgtct catcttgtaa tttctgtgct
    12721 agattaacgg atttactatc agcgagattg cttagtccac ctgcacgctt aatcgcatct
    12781 gttacacgac tttgagaagg caaactataa acacctggat gattgactgc cccttttacg
    12841 tcaaccgtta cttgattaga tactttatct ttgacgtgac taatcattgg tacctttttt
    12901 tctgttatag ttgatacttt tttctctaaa ttgatattta aatcgtcttc tttatgatga
    12961 gtgccaaaat gatttaaacc caaaattcca aacaataaga gtcctacaat aattcctgtt
    13021 gtctcccatt tatgcgattt aattttttcc aatactattt caaacataaa aatattcctc
    13081 catttactta ttcgtaacta aagagatttt cctattaaaa acaaaaaaaa ctatttacta
    13141 aaaagtaaat agtttccatg taacccttat tttagatgtt tttgtggatc ccacacaaag
    13201 cttgctaaat aactaaaaat tagagttagg acaagcaaga caatagctat aattgctaaa
    13261 ggtagacgat aaatataagt caatggattt aatttcttac catcatcata agtttcattt
    13321 tctctatcta aacgatcaaa ttcttcctga atacgacgag atacttctgc aatcccttcg
    13381 gcatccatac gcttaatatc agaaacatca atagggttac caaaattcat atcaacacga
    13441 tgccccttta atagattttt aaaaaccata ggacctctat atgctgctgg cataattctg
    13501 acttttgcca tttttgcaat aacagctacg cctcctttga catctttgga gtggcggctt
    13561 ccactcggaa acatcaccaa agaacggtta ctatttttca gcatctttac aggatatcga
    13621 atagcgtctt gccctggctt ttcacgatca atagggaaag caccgcacat tttaatccac
    13681 caaccaaaca aacgattcgt gaataattct tttttggcca taaaaacaaa ctgtttgggt
    13741 ctagcagcaa aagccatgta cacaggatcc caaaatgttc tgtgtggagc tactaaaata
    13801 taattttcat catcttttaa cattttatct tcattatgat agtgcgcatt accattagcc
    13861 acccaaatca agaacataac taaagtacgt aagtaagtat agaacatgtt tctcctctac
    13921 ctttcatcaa gtaacttcta ttttatcatg cttccttttt tttagaaaga attatcttaa
    13981 aatcaacctc cacactttca aaactagtgc taggaagctg taattttgct ataataagcc
    14041 tatgatagat acaattttaa aagaaaatga acgcattgat cagctttttt caactgatgt
    14101 caaaatcatc caaaacaaag aagttttttc ctactccata gatagtgttt tattgtctcg
    14161 gttccctaaa cttccttctc gtggtcttat cgttgactta tgcagtggta atggtgctgt
    14221 cggtttgttt gccagcacca aaacaagtgc gacaattata gagatcgaac tacaagagcg
    14281 ccttgcggat atggctaagc gttcaattaa gctcaataaa ttggaaaaac aggttactat
    14341 gattaatgat gatttaaaaa atcttcttga tcatgttcaa cgttcaaacg ttgatttaat
    14401 gctttgcaat ccaccttatt tcaaagcaag cgaaacctct aaaaaaaatc taagcccgca
    14461 ttatctcctt gcaagacatg aaataacaac aaaccttaga gagatttgtc aaattgctca
    14521 acatgctcta aaaacaaaag gacgtatcgc tatggtacat cgtccagacc gtttccttga
    14581 aattattgat acgatgcatc agttcaattt agcacctaaa cgtatccaat ttgtttatcc
    14641 aaaattagga aaggatgcta atatgctttt aattgaagct attaaagatg gctcgacaga
    14701 aggtatgaaa atacttcctc ctcttgttgt tcatcaagat aatggagact atactgagac
    14761 tatttttgat atatatttcg gggaaaacgg aaaatctcat gattaaagtt cctgcttata
    14821 tgtatgtttt agaatgttca gatggaacac tttatacagg ttatactaca gatgtcaagc
    14881 gccgtctcaa tacacacaat accggaaaag gtgctaaata tactcgtgcg agattgccag
    14941 taaaacttct ttattctgaa gcttttaata gcaaacaaga agcaatgagg gcagaagcat
    15001 tatttaaaca aaagactcgg aaagcaaaat tgacttatat taaacaacat aaaaatg
//
LOCUS       DATRME010000048        14877 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRME010000048 DATRME010000000
VERSION     DATRME010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..758
                     /locus_tag="VBC23_001422"
     CDS             <1..758
                     /locus_tag="VBC23_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6843365.1"
                     /translation="KIPIGGPLLEFGPILTFFLTVLWIISITNAINLIDGLDGLVSGV
                     SIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFPYNYHPAIIYLGDAGALFIG
                     FMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVRRKLSGKKISEADKMHLHHR
                     LLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIFLLLALLLVMEIFIEGLNIW
                     GENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            923..1693
                     /gene="sufC"
                     /locus_tag="VBC23_001423"
     CDS             923..1693
                     /gene="sufC"
                     /locus_tag="VBC23_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6843366.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDGKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            1730..2992
                     /gene="sufD"
                     /locus_tag="VBC23_001424"
     CDS             1730..2992
                     /gene="sufD"
                     /locus_tag="VBC23_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6843367.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            2994..4226
                     /locus_tag="VBC23_001425"
     CDS             2994..4226
                     /locus_tag="VBC23_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6843368.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            4213..4656
                     /locus_tag="VBC23_001426"
     CDS             4213..4656
                     /locus_tag="VBC23_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6843369.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            4756..6174
                     /gene="sufB"
                     /locus_tag="VBC23_001427"
     CDS             4756..6174
                     /gene="sufB"
                     /locus_tag="VBC23_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6843370.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(6245..7432)
                     /locus_tag="VBC23_001428"
     CDS             complement(6245..7432)
                     /locus_tag="VBC23_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6843371.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(7585..8820)
                     /locus_tag="VBC23_001429"
     CDS             complement(7585..8820)
                     /locus_tag="VBC23_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6843372.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            9062..10720
                     /locus_tag="VBC23_001430"
     CDS             9062..10720
                     /locus_tag="VBC23_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6843373.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            10835..11749
                     /locus_tag="VBC23_001431"
     CDS             10835..11749
                     /locus_tag="VBC23_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843374.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            11759..12790
                     /locus_tag="VBC23_001432"
     CDS             11759..12790
                     /locus_tag="VBC23_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843375.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            12803..13852
                     /locus_tag="VBC23_001433"
     CDS             12803..13852
                     /locus_tag="VBC23_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843376.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            13849..14781
                     /locus_tag="VBC23_001434"
     CDS             13849..14781
                     /locus_tag="VBC23_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6843377.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         4741 a         2440 c         2986 g         4710 t
ORIGIN      
        1 ttaagattcc aataggtggt ccactcttgg agtttggtcc tattttaact ttctttttga
       61 cagttttatg gattatttca atcactaatg ctatcaatct gattgatggt ctagatggtc
      121 tagttagtgg tgtttcaatc atcagtctag caacgatggc agtggtttct tatttctttt
      181 tacctaaaat agattttttt ctcacgctaa ctattgttat tttaatcgcc tcaatagttg
      241 gttttttccc ttataattat catccagcca ttatctacct aggtgatgct ggtgccttgt
      301 ttataggatt tatgattggt gtattatcgc ttcaaggttt aaaaaactcc acagctgttg
      361 cagtgataac acctgtcatt attttaggag ttcctatttt ggatacagct gttgctattg
      421 tgaggcgtaa attatcaggg aaaaagattt ctgaagctga taagatgcac ctgcatcacc
      481 gattgctatc tatgggattt acacatagag gagcagtttt agttgtttat ggtattgcaa
      541 ttatcttttc gcttatcgct ttgcttttaa atgtttctag tcgtattggg ggaatttttc
      601 ttctattggc tctactatta gtaatggaaa tttttataga aggtttaaat atttggggtg
      661 agaatcggac accgctattt aatctcttaa agttcattgg caacagtgat tacagacaaa
      721 gtgttattgc taaatatagc gataagcatc aaaaataaaa gcctgaaaag gcttttttag
      781 ttttaatcat ttagatagca gtcacacctc atttttgcta taattattta gaatataata
      841 tcgggtgagt gccctataat atcttcttta aaccatagag aaatatcaca tattctctgt
      901 taaccaagaa aaggagtgta caatgtctgt acttgaaatc aaaaatcttc atgtttctat
      961 tgaagataaa gaaattttaa aaggtcttaa tttaacgctg aaaacaggag aaattgctgc
     1021 tatcatgggt ccaaatggta ctgggaagtc gacactttca gcagctatta tgggaaatcc
     1081 taattatgaa gttacagcag gtgaaattct ttttgatggt gaagatattt tagagctaga
     1141 agtggatgaa cgtgctcgtt tgggtctatt tcttgcgatg caataccctt cagaggttcc
     1201 agggattact aatgctgaat ttatccgagc agctatgaat gcgggaaagg ctgatgatgg
     1261 taaaatttcc atccgccaat ttattactaa gttagatgaa aagatggaac tacttggcat
     1321 gaaagaagaa atggcggaac gttatcttaa cgaaggtttt tcaggtggtg agaagaagcg
     1381 caatgaaatt ttacaactcc taatgttaga gcctaagttt gccctcttgg atgagattga
     1441 ctctggcctg gatattgatg ctcttaaagt ggtatcaaag ggagtcaatg aaatgcgtgg
     1501 tgaaggtttt ggtgctatga ttattacaca ctatcaacgc cttttgaatt acattacacc
     1561 agataaagta cacgttatga tggacgggaa ggtagtattg tcaggaggac cagaattagc
     1621 ggttcgcctt gagaaagaag gttatgctca aatcgctgaa gagcttggtt tagagtataa
     1681 agaagaagtc taatttgtca cttcaactag aagagaaaag gagttacaaa tgtctaaaga
     1741 agctatatta aattttttac aagccaaagg tgagcctact tggttacaag aattacgact
     1801 aaaggctttt gaaaaaatag aagaactaga attaccagta attgaacgtg taaaatttca
     1861 tagatggaat ttaggtgatg gaactatttt agagaatgac tatacagcta acattccaga
     1921 tttcacagag ttaggaaaca atcccaagtt ggttcaaatt gggacccaaa ctgtccttga
     1981 acaagttcct atggaattaa tagagaaagg cgttgtcttt acagatttct attcggcttt
     2041 ggaagaaatt ccagaagtca tagagcgcta ctttgggaaa gcacgtcctt ttgaagaaga
     2101 tagacttgct gcctatcaca cagcttactt caacagtggt gcagtgcttt atattccaga
     2161 taatgttgaa attactcaac caatcgaagg tcttttttat caagatagtc aatctaaagt
     2221 accatttaat aagcatatcc ttcttattgt tggaaaaaat gcaaaagtta gttacttaga
     2281 gcgatttgag tcaattggtg atgggacaga acgaacaagt gctaatattt ctgtagaagt
     2341 aattgcccaa gcaggtagcc aaattaaatt tgcctcaatt gatcgcctcg gagaaaatgt
     2401 gacaacgttt ataagtcgtc gtggtcgtca cagtagcgat gcaacaatag attgggccct
     2461 tggggtgatg aatgagggca atgttgttgc tgactttgat tctgatttaa ttggtgatgg
     2521 aagccacgct aatttaaaag tagttgctgc ttcgtcaggg cgtcaagttc aaggaattga
     2581 tacgcgtgtt acaaactacg gctgtaattc agtaggacat attttacagc atggggttat
     2641 tttagagcgt gggactctta cttttaacgg tatcggacat attataaaag gtgcaaaggg
     2701 agcagacgcc caacaagaaa gccgagttct catgttatca gacaaagcac gttcagatgc
     2761 taatcctatc cttttgattg atgaaaatga tgttacagct ggtcacgcag cctcaattgg
     2821 tcaggttgac ccagaggatt tatattattt gatgagccgt ggcttgaacc aaaaaacagc
     2881 agagcaatta gttattcgtg gatttttagg aacagtcatt gctgaaatac cagtaaaaga
     2941 agttcgcgat gaaatgattg ccgttattga tacaaaacta gaaaaacgtt aatatgacat
     3001 tactagatag ttataaacta aagcaagatt tcccaatttt aaatcaatta gttaatgatg
     3061 agccgcttat ctatttagat aatgcagcaa cgacacaaaa accgaatcaa gttttagaaa
     3121 ctttgcgaga ttactaccaa aatgacaatg caaatgtcca tcgtggtgtt catactttag
     3181 cagaacgagc gactgctcag tacgagactg ctcgtgaaaa agcaaggcag tttttaaatg
     3241 ctaaatcaag taaagaaatt ttattcacac gtggcaccac aacaggatta aattgggtag
     3301 ctaagtttgc cgaaagtatt ttagaacgag gtgatgaggt acttatttcc attatggagc
     3361 atcattctaa tattatccca tggcaacaag cttgcgagag aactggtgct aaattggttt
     3421 atgcctatct aaaagatggt tctttagatt tagaagattt ctataataaa ttatcctcta
     3481 aaactaaatt tgttagtttg gcacatatct caaatgtact tggctgtatt acaccagtaa
     3541 aagcaatagc agaacgtgtg catcaagttg gggcatacat ggttgttgat ggagctcaat
     3601 cagcgccaca tatggcgatt gatgttcaag accttgattg tgatttcttc gccttatctg
     3661 gccataagat gcttggtcct actggtattg gtgttcttta tgggaaagaa agcattttag
     3721 ataagatgcc acctgtcgaa tttggtggtg aaatgattga ttttgtctat gagcatagtg
     3781 caacatggaa ggaattgccc tggaagtttg aagctggaac gcctaatatt gctggagcga
     3841 tcgcttttgg agaagcttta gattatttaa cggatgttgg tatggatgag attcatcaat
     3901 atgaacaaag tttagtttct tatgtgttgc ctaaattaca ggctatcgat ggtttaacta
     3961 tttacggacc tagtgatgct gaatctcatg taggcgttat tgccttcaat ttagaaggtt
     4021 tacacccaca tgatgtggca actgcaatgg attacgaagg agtggctgtt cgtgcaggtc
     4081 atcattgtgc acaacccctt attaatcatc ttggtatcca ttcggcggtt cgagcaagtt
     4141 tctatttcta taataccaaa gaggattgcg acaaattggt agacgctatc caaaaaacta
     4201 aggagttttt caatggcact ctctaaatta gataacctct acatggcggt ggtagctgat
     4261 cattcaaaac accctcatca tcatggattt ttagaaggcg ttgaacaagt gcagttaaac
     4321 aatcctacat gtggagatgt tatttcctta tctgttaaat ttgatgggaa tattatttca
     4381 gatattgcat ttgctggtaa tggttgtaca atttctacgg cttcatcaag catgatgaca
     4441 gatgcagtta ttgggaaaac aaaagaagaa gcattgcagc ttgctgatgt attctccaaa
     4501 atggttcaag gcaatcaaaa tccaaaacaa gagaaacttg gggatgcaga gtttctagct
     4561 ggggtatcaa aatttccaca acgtatcaaa tgtgctacgc tttcgtggaa tgctttaaga
     4621 aaggctattg agagagataa tcaggcagaa ctctgacaac catagttgaa ggaattacat
     4681 ttaaacaact tttactgaca taaggtagtc tagtagaaga atgtcttaaa tttaaatatt
     4741 aaagaaagga taagtatgtc cgaaataaat gaaaaagtag aaccacagcc aatagatctt
     4801 ggggaatata aatttggatt tcatgacgat gtaaagccaa tttattctac gggaaaaggt
     4861 ctaaatgagg ctgttattcg tgagttatct gcagctaagg gtgaacctga gtggatgttg
     4921 gactttcgtc taaaatcctt ggaaacgttt aataaaatgc cgatgcagac ctggggagca
     4981 gatttatcag atattgattt tgatgatatt atttattatc aaaaagcatc tgataaacct
     5041 gcgcgtgatt gggatgatgt tccagaaaaa atcaaagaaa cttttgaaag aattgggatt
     5101 ccagaagctg aaagagccta tcttgcagga gcatcagcac aatatgaatc agaagtagtt
     5161 tatcacaata tgaaagaaga atatgataag ctgggtattg tttttacgga tactgactct
     5221 gcacttaaag agtacccaga gctattcaaa aaatattttg ctaaacttgt ccctccaaca
     5281 gataataaat tagctgctct gaactctgct gtatggtcag gtggaacatt tatttatgtt
     5341 cctaaaggtg ttaaggtgga tattccactt caaacttact tccgtattaa taatgaaaat
     5401 actggacaat ttgaacgtac tctcattatt gttgatgagg gagcaagtgt tcactatgtt
     5461 gaaggttgta ccgccccaac ttattcttca aatagtttac atgcagctat agttgaaatt
     5521 tttgcacttg atggagctta tatgcgctat acgactattc aaaattggtc cgataatgtc
     5581 tataatttag tgacaaaacg tgctaccgct aaaaaagatg caacagttga gtggatagat
     5641 ggaaatctag gagctaaaac aacaatgaaa tacccatcgg tttaccttga tggtgaagga
     5701 gcacgtggca cgatgttgtc tattgctttt gcaaacaaag gacaacacca agatacgggt
     5761 gcaaagatga ttcataatgc cccccatact agttcatcca ttgtctctaa atcaattgct
     5821 aagggtgggg gaaaagttga ttatcgaggt caagtgacat ttaataaaga ttccaaaaaa
     5881 tcagtgtcac atatagaatg tgacaccata ttgatggatg atatttcaaa atcagatacc
     5941 ataccgttta atgagattca taattcacag gttgctttag agcatgaagc aaaggtgtct
     6001 aagatttctg aagagcaact gtactacttg atgagtcgag gtttatctga agctgaagca
     6061 acagaaatga ttgttatggg gtttgttgag ccctttacga aagaattacc aatggaatat
     6121 gcggtagagt taaatcgttt aatttcctat gaaatggaag gttcagttgg ttaattgaag
     6181 atttcaagag ataataaaaa gcccctatgt tattgatgaa ggggctttta tattagcaca
     6241 gttattatct agagcgcttt ataagtaata ctatcccagc taaaagacaa aggctagcga
     6301 tggtgcccag aacaattaaa atagatacaa aatgtgattg gataaattga tctgtttttg
     6361 tccaccactg ttcttttgtt tgaggctgtt cttgaataga tttagttttg gttttagtaa
     6421 cttctttttt aggtttaacg gcttttaata caggagtttt agaagaaata tttttagcgt
     6481 ctttaaaacc tttttctaca agagcgttga caaacggatg acgatagtac tctccgtctt
     6541 ggtctgacca atcgccaact cctaaaacca cagttatcaa gcgagtattc tggcgtttag
     6601 ctgtaactaa ggcattaaaa gcagcgctag ggctagaacc agtttttaag ccatctactc
     6661 cttctaatcc aaatttagcg ccgggggtag agtagttata agttgtaaat gtttcttcat
     6721 aaggagttcc gaccatggcc ttgacttcag gatattttgt atagttcagt atatcagggt
     6781 attttttaag gaaatgatag gttaaaattg atagatcacg tgcagtcgta acgttagtag
     6841 cattgttatc gtattctttt ggggagtaaa gtccattaaa agcactcgct accgccccac
     6901 tggggttata aaagtgagtt tttgtcatac cgagtttctt ggcggtttca ttgattcgtt
     6961 taataaaggc gtcaggattg ttttgtgata agtggttagc aatcataata gttgctacat
     7021 tagatgacgg gacagccgtc atagtaatca gttcacgaat aggataagca accccagcat
     7081 gaatattgtt attactaatt tcataaatct tacttatggc ttggtctgtt tctgttgcag
     7141 tcactgtggt gttgaggttt gtttttcctt tagctaagtc ttcaaatagt aaatataaag
     7201 taaacatttt agacatgcta gcgggatcac gttctaaatc ggcgttatct tcccacaaaa
     7261 tatgaccttt attgtcaata acgatagacg ctttaggttt attaatatct ttaacatcat
     7321 atccggcttc tcgggtaatt tgcataatat ctttatgttc ttcagcttgc acagagttta
     7381 cacaagtcag ggataataaa atgcagagga aagatacgat taatttaggc ataggtaact
     7441 cctgaaaact ttttattaat attataacaa aaaatcacta ataaaaaaga catttcatta
     7501 gtgattttta attttatata ttctcctaaa cctagctaaa gaaactgctg tgcagtaact
     7561 ccttgtgtca aagggagatt actattaaag tttttcgtta acgtagcgca caaaatgatt
     7621 ccaccacact ttgaggaaga aactgcgctc aatactatct tttgccacta aatgaacgct
     7681 aggttccttt atgagatagc cttttccaac cttattaagg tcaacgtaat aagctttccc
     7741 taggttctct ttttttgtaa taggagcagt aagctctttt ttaaagttaa tttttaactg
     7801 attttgttta gtgatatttt tcttctgtac tactttcaaa tcgtttttag caacagcgat
     7861 gaccgattgt tcttttccgt ctctcacact tgcttcttta cctttatatg cagctccttt
     7921 agctaataca aggttaggtt cgtaggtgtt tgtgatatag ttcaagagag agttagttgc
     7981 tgtaaagcga gcatatttgt ctttatcggc cttatcagca tgcataataa cagtgagtag
     8041 tctcattcca ctttcagtag atgtagcaat aaaagattgg ccagctaatt ccgttgtccc
     8101 agttttcaaa cctgtaatac ctggtctaaa gacaggcata tctggtagca tgtagttata
     8161 agaatgcata atatctttat caaatttagc aacggactta ctagtaatct taagaatgga
     8221 aggatattcg ttgaccaaat ggtaggcagc aatagcaata tcacgtgcac tcattttatt
     8281 ttcgtcgttt tgtgacgatt ttggataaat gtgattgcct aacatactat tatttaagcc
     8341 agaagcattg actaggtggc tatcatgaat tccccacttt tccaattgag cagtcatttt
     8401 atcaacaaat ttactttcag ttcctgaaat atgttcagct aaagcaatgg ctgcactgtt
     8461 agcactagaa atcatggcag cgtccacgag ttgtttaaca gtatagcgcc ttttttctaa
     8521 aggaacatta ctagcatcag attcgcgtgt tagttgataa gggtagtcag atatatttac
     8581 tttggtattc cacttgaggt taccgttatc aatttcttta tagaccatat aaacggtcat
     8641 tattttagtc aaggaagcaa tagcagcggg tttgttagca tctttttcat acaagatttt
     8701 tcctgaatct aaatcaactg ctaaggcatg tttagcggaa gcattaaaag attcagcaga
     8761 gatggttggc ataaaaaaaa tgcaactaag taatagaata gaagtaataa tttttttcaa
     8821 aataacacct ttagaaattt tttaaacttt gtccctccta ttataccata tcactatgtc
     8881 atactaggaa ttattatttt cagataattg aatgaagaaa acgtttgtta taaaaatgta
     8941 aaaaaatgta aaaaaatgaa agttaaaaaa ttatctgaaa attttgcgtt tttgtctttt
     9001 cttaagaatt ttttgtggta taatgttagt cactagataa atgaattagg agaacaaagc
     9061 tatggcaaaa cagaaaaata actggcgccg tgttggagtt ggtgtcctta cacttgcttc
     9121 agttgcgact cttgctgcat gtggaagtaa atcagcttcc caggattcta atggagcgat
     9181 taattgggct attccaacag aaatcaatac actagattta tctaaagtta cagacactta
     9241 ctcaaatcta gctattggta actctagtag taatttcctt cgcttagata aagatggaaa
     9301 gacaagacca gacttggcta ctaaagttga tgtttcaaaa gatggcttaa cttatacagc
     9361 tacattacgt aaaggcttga agtggtcaga tggcagtaaa cttactgcaa aggattttgt
     9421 ttattcatgg caacgtttag ttgatcctaa aacagcttca caatatgctt accttgctgt
     9481 tgaagggcat gtgcttaatg ccgataaaat caacgaagga caagagaaag acttgaataa
     9541 gctaggtgtt aaggcagaag gcgatgacaa agttgttatt actttatcta gtccgtctcc
     9601 gcaattcatc tactaccttg cattcactaa cttcatgcca caaaaacaag aagttgttga
     9661 aaaatatgga aaagattacg caactacttc aaaaaataca gtttactcag gaccatatac
     9721 tgttgaaggt tggaatggtt cgaatggtac tttcacgctg aagaaaaaca aaaattattg
     9781 ggacgctaaa aatgtaaaaa caaaagaagt tcgcatccag actgttaaaa aaccagatac
     9841 cgccgttcaa atgtataaac gtggtgagtt agatgcagct aatatctcaa atacttctgc
     9901 tatttatcaa gctaataaaa ataataaaga tgtcacagat gttctagaag cgaccactgc
     9961 ctatatggaa tataatacta ctggttctgt gaaagggctt gataatgtta agattcgtcg
    10021 cgccttaaac ttagcaacta accgtaaagg agttgttcaa gcagccgttg atacaggctc
    10081 aaaaccggca attgcttttg cacctactgg tttagccaaa acaccagatg gaactgattt
    10141 ggcaaaatat gttgccccag gttatgaata taataaaact gaagcagcaa aactctttaa
    10201 agaaggtttg gctgaatcag gcttgactaa gctaaaatta acaattacag cagatgctga
    10261 tgctcctgct gccaaaaact ctgttgacta tatcaagtct acttgggaag ctgctcttcc
    10321 aggacttact gttgaagaaa aatttgtaac ctttaaacaa cgcttagaag acagtagaaa
    10381 acaaaacttt gacattgtag tttctctttg gggtggggat tatccagaag gatcaacttt
    10441 ctacggcctc tttaagtcgg attcacaaaa taacgatgga aaatttgcta acaaggacta
    10501 tgatgctgct tataacaagg caatttctga agatgcgatg aaaccagcag aatcagcgaa
    10561 ggactataaa gaagcagaga aaattttatt tgagcaaggt gcttataacc cactctactt
    10621 ccgtagtggt aaaggtttac aaaatccaaa attaaaaggt gttattcgta atactacagg
    10681 tttgtcaata gactttacac atgcttataa aaataattaa attactttac actaaacctt
    10741 tgcaagaatt ggtaattgcc agttcttgct tttggttaag taaagaaagt gaaagagttt
    10801 gctattttta gcaattctaa gaaaaggaaa tgatatgatt aaatatattt taaagcgtgt
    10861 tgctattttg ttagtaacgc tatgggttgt cataacccta tcgttctttc tgatgcagat
    10921 ccttccagga acaccctaca ataatccaaa actcactgaa gaaatgatca cattgcttaa
    10981 taaacagtat ggtttagaca agcctgtatg gcaacaatat ttgacttacc tttggaatgt
    11041 acttcatggt gattttggaa cgagttatca atcagtgaat cagccagtat cacgtatgat
    11101 tagcttgaga ttaggagttt cagtacatct aggtgttcag gctctcgtat ttggtgtttt
    11161 aggcggtatt ttggtaggag ctatttcggc acgtcataag aatgataaag tcgatggtat
    11221 cttaagtgtt attgcaacgc tagggatttc tatgccttca tttatcattg gtatcttatt
    11281 attagactat tttggattta agtggaactt gttgccttta tcaggttggg ggactttctc
    11341 tcaaacaatt ttaccttctt tagctctagg actccctaca ttagcatctg tttcacgctt
    11401 cttccgtagt gaaatgattg aaactttaaa ttctgattat gtgcaactag ctcgttcaaa
    11461 aggtatgaca attcgtcaag taactcgaaa acatgcttac cgtaattcta tgattccaat
    11521 tttaactcta attggccctt tggcggcggg tctgttaact ggttctgcac taattgagca
    11581 gattttctcg attccaggaa ttgggcagca gtttgttact tcgattccaa caaaagacta
    11641 tcctgttatt atggggacaa ccattgttta tgcagtaatg ttgatggttg ctatcttgat
    11701 aacagacgtt gttattagta tcgtagaccc acgtgttcgc ttgcagtaag gagatgttat
    11761 ggcagataaa aacagaacat ttaaacttgt aggtgcagga tcttctagca cacaagaaaa
    11821 aattgaaaag cctgctcttt cgtttatgca agatgcgtgg cgtcgcttga aaaaaaacaa
    11881 attagcagta gtttcactct atttattagc tcttttactt actttttcgt tagcctcaaa
    11941 tttatttgta actcagaagg atgctaatgg gtttgattcg aaaaaagtaa cgacatatcg
    12001 caacttacca cctaaattga gttcaaacct tcctttttgg aatggtagca ttaagtacgc
    12061 tggaaataca gaatctacag acgcttataa gtctcaaaat gtccctgaaa aagttaagta
    12121 tgctttaggt acagattctc tagggagaag tgttgctaag cgcatcatcg taggtattcg
    12181 aatttcctta cttgtagcta ttgcagctac attcattgat ttaattatcg gtgttacata
    12241 cggtcttgtt tcaggttttg ctggaggacg ccttgataca ttaatgcaac gtgtcgtaga
    12301 ggtaatttca tcaattccaa acttagttat tgttacaatg ttaggtttgg tccttggtaa
    12361 cggtattacg gctattatta tctctattgc ttttaccgga tggacttcta tgtctcgtca
    12421 ggtaaggaat ttaaccctat catatcgaga aagagagttt gtgcttgctg cacgttcact
    12481 tggggaaagt ccaattaaaa tagccttcaa acatatttta cctaacattt ctggtattat
    12541 cattgtacaa attatgatga caatcccaag tgctattatg tatgaagcgg tactttcagc
    12601 aatcaattta ggtgttaaac caccgacagc ttcattagga tcattgattt cagatgcaca
    12661 agaaaattta caatattatc catatcaagt tatcttgcca gctttagcat tggtaatgat
    12721 ttcattggca tttattttgc ttggtgatgg gttgcgagat gcttttgatc caaaatctag
    12781 cgatgaataa ggaggtataa tcatggaaaa agaaactatt ttaagtgtta ataatcttca
    12841 tgttgacttc cacacatatg ctggagaagt aaaagcaatt cgtgatgtca actttgaatt
    12901 aaaaaaaggt gagactcttg caatcgttgg tgaatctggt tcaggaaaat ctgtaactac
    12961 cagaacttta attggtttaa atgctaaaaa ttcagagata tcagggaatg ttcaatttaa
    13021 ggggcgtaac cttgttgaac tatcagaaga agagtggact aaggtacgcg ggaatgagat
    13081 ttctatgatt ttccaagacc ctatgactag tttggatcca acgatgaaaa ttggtatgca
    13141 aatagcggag ccaatgatga ttcatcaaaa aatttcaaaa aaggatgctt taaaattagc
    13201 tcttgagcta atgaaagatg taggtatccc aaatgctgaa gagcatatta acgattaccc
    13261 acatcagtgg tctggaggaa tgcgtcaacg tgcggttata gcgattgctt tagctgctga
    13321 tcctgaaatt cttattgctg atgaaccaac gactgcttta gatgtaacaa ttcaagcaca
    13381 aattttaaac ttaatgaaaa aaattcaagc agaacgagac tcctccattg ttttcataac
    13441 tcatgactta ggggtcgttg cagggatggc agaccgtgta gcagttatgt atgcagggaa
    13501 aatcgttgaa tttggaactg ttgatgaagt cttttataat ccacaacatc catatacttg
    13561 gggattattg aattcaatgc cgacaaccga aacagaatca ggtagtttag agtcaattcc
    13621 gggaacacca ccagatttgt tgaacccacc taaaggagat gcatttgcag ctcgtaatga
    13681 atttgcatta gatattgatc atgaagaaga accaccgtac ttcaaagttt cagaaacaca
    13741 tttcgctgct acatggcttt tagatgaaag atcacctaag gtgcttcctc cattaccgat
    13801 ccaaaaacgt tgggaaaaat ggaacgagat tgaagggagg aaagcccaat gactgaaaat
    13861 cgaaaaaaat tagttgaagt caaaaatgtt tctttgacct tcaataaagg aaaagctaat
    13921 gaagtgagag caattgataa tgttagtttt gacatttatg aaggtgaagt gtttggatta
    13981 gttggggaat ctggatcagg aaagacaacc gttggacgtt caattttaaa actttatgat
    14041 atttctgatg gcgaaattac ctttaatgga gaagtgatct cacatctgaa aggtaaagca
    14101 ttacatagtt ttcgtaaaga cgctcagatg atttttcagg atcctcaggc tagcttaaac
    14161 ggccgtatga agattcgtga tatcgtagca gaaggtttag acatacataa attagctaaa
    14221 tcaaaatcag atcgtgatag taaagttcag gccctattag atcttgtagg gttaaataaa
    14281 gatcatttaa cacgttatcc gcatgaattt tcaggtggac aacgacaacg tatcgggatt
    14341 gcacgtgctt tagcagtaga gcctaaattt atcattgctg atgaaccaat atctgcctta
    14401 gatgtttcaa tccaagcaca agttgttaat ttaatgcaaa aattgcaaag agaacaagga
    14461 ttgacttatt tgtttatcgc acatgatttg tctatggtaa aatacatttc agatcgtatc
    14521 ggtgttatgc attgggggaa actgttagaa gttggaacat ctgatgatgt ttataacaaa
    14581 ccaatccacc cttatacgaa gagtctatta tcagctattc cagaaccaga tccggagagt
    14641 gaacgtcaac gtgttcacca gccgtataat ccagctattg agcaagacgg acaagaacgt
    14701 caaatgcacg agattacacc tggtcatttt gttttatcta caccccaaga agctgaagaa
    14761 tataaaaaac aaatattata aaagatggct agcccatctt ttattttttt actaataaaa
    14821 taaatttagg tatggaattt taaactctat gttattggtg ttagccaaca ggaaact
//
LOCUS       DATRME010000049        14621 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRME010000049 DATRME010000000
VERSION     DATRME010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..809)
                     /locus_tag="VBC23_001435"
     CDS             complement(<1..809)
                     /locus_tag="VBC23_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6843378.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
     gene            complement(809..1711)
                     /locus_tag="VBC23_001436"
     CDS             complement(809..1711)
                     /locus_tag="VBC23_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6843379.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(1717..1845)
                     /locus_tag="VBC23_001437"
     CDS             complement(1717..1845)
                     /locus_tag="VBC23_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843380.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1978..3021
                     /locus_tag="VBC23_001438"
     CDS             1978..3021
                     /locus_tag="VBC23_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6843381.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            3125..5287
                     /locus_tag="VBC23_001439"
     CDS             3125..5287
                     /locus_tag="VBC23_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6843382.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            5250..5708
                     /locus_tag="VBC23_001440"
     CDS             5250..5708
                     /locus_tag="VBC23_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6843383.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            5789..6052
                     /locus_tag="VBC23_001441"
     CDS             5789..6052
                     /locus_tag="VBC23_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6843384.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            6280..7215
                     /locus_tag="VBC23_001442"
     CDS             6280..7215
                     /locus_tag="VBC23_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6843385.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            7208..7981
                     /locus_tag="VBC23_001443"
     CDS             7208..7981
                     /locus_tag="VBC23_001443"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6843386.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7996..8394
                     /locus_tag="VBC23_001444"
     CDS             7996..8394
                     /locus_tag="VBC23_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6843387.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            8391..8822
                     /locus_tag="VBC23_001445"
     CDS             8391..8822
                     /locus_tag="VBC23_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6843388.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(8863..9138)
                     /locus_tag="VBC23_001446"
     CDS             complement(8863..9138)
                     /locus_tag="VBC23_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6843389.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            9481..10407
                     /locus_tag="VBC23_001447"
     CDS             9481..10407
                     /locus_tag="VBC23_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6843390.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            10537..11823
                     /locus_tag="VBC23_001448"
     CDS             10537..11823
                     /locus_tag="VBC23_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6843391.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRIEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            11950..12162
                     /locus_tag="VBC23_001449"
     CDS             11950..12162
                     /locus_tag="VBC23_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO6843392.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            12286..13083
                     /locus_tag="VBC23_001450"
     CDS             12286..13083
                     /locus_tag="VBC23_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843393.1"
                     /translation="MIRHSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
     gene            complement(13185..14525)
                     /locus_tag="VBC23_001451"
     CDS             complement(13185..14525)
                     /locus_tag="VBC23_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6843394.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
BASE COUNT         4908 a         2230 c         2894 g         4589 t
ORIGIN      
        1 aagacacgat aaacataagg attattaggt ttagaaactt gacgagactc ttcaatgtgt
       61 aagactggta agttttgttg taacaattga ttatactcgg atttaattgt acctctaaca
      121 gttacccaag tattgtccgg ataacttttt tgatttccag ttgttagtag accataaact
      181 cctgaatcag caatacaatg aatgattcca aagcggaaaa taaattgata tccttcgtgt
      241 ttgggttcgt tataaacaaa cccagtatat tgtatttggc gatccatgaa ttgctcggga
      301 tagagataaa ttagttccat aacttccatg tagttttcag ttgtgatggt taatgtacca
      361 gagcctttat attttttaag ctctttttgc atttcttttt cataggctga agaagtaaaa
      421 taagtgctgg tatctggttt caaatattga acgcgtgtcc catcttgact gacagtacca
      481 gtagagccgg ctgctagagg gaaattatat cccttggcag agactgtggt cgagtctaaa
      541 ctaactgtag gtactaacaa acctactaaa acgggaaata ctaaaatcat tgggcttgtt
      601 gatttagcta ttttaccatg taggtgacta tgcattttca tatttttaac ccaaataatt
      661 agttgtacaa ttgctaaaat aaaagatagg accattgaaa tataggccaa ataggtataa
      721 tgagtattaa tatactgatt tagtttacct gataacttta aatacatgct taattcaaaa
      781 taaccagcaa gaataagaaa tcgaatcatt atattacccc cacaaataaa caatagataa
      841 taataataag agaagataca ctaatgaatt ggacaataaa ccttgtttta aaagaattta
      901 ccatcatcat taaattctta atatcaatca tgggtccaat taaaaggaaa gccatcactg
      961 gcgcgattcc aaaggttgat aataaagaag cacctataaa agcgtcagct tcactacaca
     1021 atgacaagat gaaagcaagc aacatcataa ctaagatggc tgtgataggg ctgtgtccga
     1081 tagtagtaag aactcgagtt ggtaaataaa tttgcatagc tgaagcaatt aaagtgccaa
     1141 atactagata gcgtcctgtg tcaaaaaatt catcgatggc atgagctaag gctagaaaga
     1201 ttttttgata ccacttctta tcagaataat catgaaaatg agtaggttta gcgtcttctt
     1261 ttagaatatt atcatctact aaaaaggcaa gcatgactcc taaagcaatg gctacaatgg
     1321 tagcacctac aaagcgtaat atcaggaagc gtatagaatt tccaaatgct gagtaggtcg
     1381 caaataaaac aataggatta ataataggag cagtagcaag aaacggaact gccgtgtatg
     1441 aaggcacttt cttttcaagg aatcgattaa taataggaat gataccacat tcgcaagaag
     1501 gaaagacaaa gccaacgaag gttccaaata agactctgag aaatttattt ttcggtaaga
     1561 acttattgac tatatcaggt gttataaaga cttcaataat tcctgataaa atagttccaa
     1621 gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa aatggcaaac cattgaagga
     1681 cactatccgg taattgatta aagatatcca tagaacttaa tctttctgag aatcatcttt
     1741 ctgttcaggt tttgaatcag aatcaacttt tttaggtttt ggtactttag caaaactagc
     1801 gaacatactt tctaagtcat cttgacgttt gtgcgtaata gccattaatg gcacctcttt
     1861 ctttatataa taacctatta tactatatta gcttattttt aacataaaaa aacaaatcga
     1921 ttggaaatga tactatatag tgataaaatg ttattagtag attgatgagg taagattatg
     1981 attgaattag gtattacaac attcggagag acaacaatcc ttgaagaaac aaaccaaagc
     2041 tattcacatc ctgagaggat tcgccaatta gttgctgaga ttgaactagc tgatcaagtt
     2101 ggtttagatg tatatggtat tggagagcac catcgtgaag attttgcggt ctctgcaccc
     2161 gaaattatcc tagcagcagg agcggttaga actaataata tccgtttatc tagtgcagta
     2221 acgattctct cttccaatga tcctattcgc gtctatcagc aattttcaac gattgacgca
     2281 ctttcaaatg gtagagcaga aattatggca gggcgtggtt cctttattga gtcttttcca
     2341 ttgtttggat acgatttagc ggattatgat gatttattta atgaaaaaat ggatatgttg
     2401 ttagcaatta actcagcgac aaatctcgat tggaaaggtc atttgacaca aacagttaat
     2461 gagcgaccaa tttatccaag agcattacaa agacagttat caatatgggt ggcaacagga
     2521 ggaaatgttg attctacaat tcgtattgca gaacaaggtt tgccaattgt ttatgcaact
     2581 attggtggga atcccaaagc ctttcgtcaa ttggtccata tttataaaga agttggtagt
     2641 cgtaatggac acaaaccaga acaactaaaa gttgctgctc actcttgggg atggattgaa
     2701 gaggataatc aaaccgctat tgaccgttat tttttcccta cgaaacagac cgtcgataat
     2761 attgctaagg gacgccctca ttggtctgaa atgactaaag agcagtattt acgttcagta
     2821 ggtccagaag gtgctatttt tgtaggaagt cctgaagtgg ttgcacataa aattatagga
     2881 cttgttgaag cgcttgagtt agaccgtttt atgttacatc taccagttgg ctctatgcct
     2941 cacaaagacg tcctaaatgc catcaaactt tatggtgagg aagtagcacc aattgttcga
     3001 aaatattttg aaaagaatta aagcagacct ggtctgtttt ttattgttag attataaaaa
     3061 cgctatcttg aaatcgcttt ttaaaactct tcgtaagtca aaaatatgct ataataaaag
     3121 cgttatggaa aatttaaata ttacacaaat tgctattgat ttaggtatta aagcgtcaca
     3181 aatcgaaaag gttttagaat taacagatga aggaaatact attcctttta ttgcccgtta
     3241 tcgtaaagaa atgacaggta atttggatga agttcaaatc aaatcaatta ttgaccttga
     3301 taagagtatg acagctcttt cagatcgtaa aactacggtc ttagccaaaa ttgaagaaca
     3361 aggaaagttg actcaagaac tcaaaaaggc tattgaagaa gcaactaaac tagcagatgt
     3421 tgaggaactc tacctacctt ataaagaaaa acgtcgtacg aaagctacga ttgcacgtga
     3481 agcaggtctt tttcctttag cacgtcttat tttacaaaat aaggataacc ttgaggaaga
     3541 agctcaaaac tatcttacag atggttttga gacaactacg aaggcccttt ctggagcagt
     3601 agatattctt atcgaagctt tttctgagga taataaatta cgttcatgga catataatga
     3661 gatctggaac tacagctcaa tcactgcagt tgttaaagat gagtcacttg atgaaaaaca
     3721 ggtcttcaaa atctactatg acttttctga aaaaataagt aaactccatg gctatcaagt
     3781 tttagcattg aatcgtggtg agaaaatggg tgttcttaag gttaattttg agcataacct
     3841 tgaaaaaatg tttcgctttt ttgctgttcg atttaaagaa actagccaat atatagatga
     3901 ccttattgta cagactgtca agaaaaaaat tgttccagct atggaacgcc gtatccgtac
     3961 agaactaagt gaaggagcag aagatggtgc aatttcactg ttttcagaga atcttcgtaa
     4021 cttattgtta gtctcaccgc ttaaggggaa aatggtgtta ggttttgacc ctgcctttcg
     4081 tacaggagca aaattagcag tagtagacca aactggaaaa ttaatgacaa cacaagtgat
     4141 ttatccagtt cctccagcta atcaagcaaa aatcgaacaa tcaaaaatag agttagcaaa
     4201 acttatcaaa gaattcaata tagaaattat tgctataggg aatggaacag cgagtcgtga
     4261 atcagaagca tttgttgcag aagttttaca agattttcca gacgtatcct atgtgattgt
     4321 taacgaaagt ggtgcctctg tttattcagc ctcagaattg gcacgtcatg aatttccaga
     4381 cttaacagtt gaaaaacgtt cggctatctc aattgcaaga cgtctgcaag acccgttagc
     4441 agaactagtc aaaattgatc caaaatctat tggtgtgggt caatatcaac atgatgttag
     4501 tcaaaaaaaa ttagcagaaa atcttgattt tgttgttgaa actgtagtta accaagtagg
     4561 tgttaatgtt aatacagcaa gtccagcctt gttagctcac gtttcaggtt taaataaaac
     4621 aatatctgaa aatattgtta aatatcgtga agagaatggg caaattaagt cacgtgctga
     4681 aattaagaaa gtacccagat taggagcaaa agcttttgag caagctgctg gattcttacg
     4741 tattcctaat gctaaaaatt tcttggataa tacaggcgtt caccctgagt cttatgaagc
     4801 tgttaaaaag ttgcttgacc aactgactat caaagaatta gatgatcttg caaaagagaa
     4861 gttacaacac cttgacttaa ttgcaacagc agaaagtatt ggggtaggtc aagaaacatt
     4921 aaaagacatt atagaagatc ttttgaaacc aggtcgtgat ttgcgtgatg attttgaagc
     4981 gcctgttttg agacatgatg ttttagatgt cagtgattta aaagttggac aggaattgca
     5041 aggaactgtt cgaaacgtcg ttgattttgg tgcttttgtt gatattggtg ttcatgaaga
     5101 tggtctaatc caccagtctc gccttattaa gcgaaaacgc gataaaaaaa ctagaaaaat
     5161 gcctccttta ccacatccaa gcaaatatct ctctgttggt gatattgtaa ctgtttgggt
     5221 tgtagaggta gatgcggagc gtggcagaat tggacttagt cttatcaagc cagacggact
     5281 tgactaatta tgttaagaag gtgtctatag aagattttgg atgtcagttt ttacatactg
     5341 cacattggaa taatcgctta agaacaaccg gaggacgctt ctttccaaac gatagacacc
     5401 ttgactttaa tcccaaaatt tatcgtgagt ttggtattga tatttttcgt aagattgtaa
     5461 gacatgagct ttgccattat catctctata tacaaggtaa aggatatcga catagggata
     5521 aagcttttaa agagttacta gaaaaagtcg atggcttacg atatacccct agagttacgg
     5581 ctgttaaagt gaattatcat cattactctt gccaatcttg tggtcaagta taccgacgta
     5641 ggagacgtgt taatttacgc aagtttgctt gtggttattg tcatggtcgc ttgatagaga
     5701 gtttttaaaa tcagtcgtaa ggcctattta attttactgt caaagtagtt ataattaaaa
     5761 tatagagtag atagaaagaa ggataggtat gaaatcaagc ttctacaaac aacgaaaagg
     5821 taagttggtc tgcggtgttg tcgccggtct agctgataag tataactggg atttagctct
     5881 ttcacgggtg ttgattgcac tgattttgta ttttaccaaa tttggtcttt tgctatatat
     5941 tttattagct gttttcttac cctataaaga agatattatt gaaacaagaa gacaaggtcc
     6001 cagaagacgt aaagatgctg aacctgttga tgatgacggt tggttctggt aattcatata
     6061 aaggagattt gctagacgaa tctccttttt tcttagcttt atttagacca ttttttcaaa
     6121 atatcttctt tttttcagaa aaacgcgtta gcgcttactt ttagtaaaaa gtatgatata
     6181 atgtgggtgt aaattaatga aagtgtttag tacctttgct caactttaaa ttgtagggta
     6241 tagcaatagg gatattcact tttataagaa ggagtaagta tggcagttac tgttcaaatg
     6301 ttagttgacc gcttaaagtt aaatgtcatc tatggtgatg aacatctatt atctaagcgg
     6361 attacaacag ctgacatttc acgtcctggt ctagaaatga cagggtattt tgattattat
     6421 gcaccagagc gtttgcaact tgttggtatg aaagagtggt catatttaat ggctatgaca
     6481 ggacataatc gttatcaagt gttacgtgag atgttccaaa aagaaacacc agctattgtt
     6541 gtagcacgtg atttagaaat tccagaagag atgtatgagg cggctaaaga taccggtatt
     6601 gctattcttc aaagtaaggc ccctacaagt cgtttatcag gtgaagtttc ttggtattta
     6661 gattcttgct tagctgagag gacaagtgtt catggtgtct tgatggatat ctatggtatg
     6721 ggtgttctga ttcaaggaga ttccggtatt ggtaagagtg agacaggact agagctagtt
     6781 aagcgaggac atcgtttggt agcagatgac cgtgttgatg tttatgctaa agacgaagag
     6841 acgctttggg gagagcctgc tgagatatta cgtcatttac ttgaaattag aggtgtggga
     6901 atcattgata ttatgagcct atatggagct agtgcggtta aagattcatc gcaagtgcaa
     6961 ttagctatat accttgaaaa ctttgaaact ggaaaagttt ttgatagact cggaaatggc
     7021 aatgaagaga tcgaattatc tggagttaag gttcctcgga tacgtattcc tgttaaaaca
     7081 ggccgaaacg tgtcagtagt tattgaagca gcggctatga atcacagagc taaacaaatg
     7141 gggtttgatg ctacccagac ttttgaggat cgtttaacac atttgattag tcagaacgag
     7201 gtcaatgatg attaatccag tagcaataca attaggcccc ttttctattc gctggtatgc
     7261 tatttgcata gtgtctggta tgttgttagc agtttactta gcaatgaaag aggcccctcg
     7321 taagaacatt aagtcggatg atattctaga tttcattctc atggcttttc cactatccat
     7381 tgttggagct cgtatttatt atgttatctt cgaatgggct tattactcta agcacccagt
     7441 agagattatc gccatttgga acggtggtat tgctatttac ggagggttaa ttaccggagc
     7501 cattctcctt gtgatttttt catatcgtcg cttgattaat cccattgatt ttctggatat
     7561 tgctgctcca ggagtgatga ttgcccaagc cataggtaga tgggggaatt ttattaatca
     7621 agaagcatac ggtagggcag taaaaaatct gaattactta ccaaacttta taaaaaatca
     7681 aatgtatatt gatggagctt accgagtacc tacattttta tatgagtctc tttggaactt
     7741 cctaggtttt gtcatcatca tgtctattcg tcatcgaccg cgcactttaa aacaaggtga
     7801 agtggcatgt ttttatttag tttggtatgg atgtggacgg ttcattattg aagggatgag
     7861 gacagatagc ctttatttgg cgggtttgcg cgtgtcacaa tggttatcag ttattttagt
     7921 tattatcgga attgtcatga ttatttatca tcgtcgtgaa caacatattt catattatta
     7981 aacagaggag gcattatgat agaaattgct gtattaatta ttgccattgc gtttgttgtt
     8041 ttagttttag gaatactttt cgttctgaaa aaagtatcag aaacgattga ggaaaccaag
     8101 caaactatta aagtattaac aagcgatgtt aacgtgactc tttaccaaac taatgaaatt
     8161 ttagccaaag ctaatgtttt agttgatgat gttaatggta aagtatctac tattgatcct
     8221 ctttttgtag ctattgctga cttatcagaa tctgtatctg atttaaacct acaggcaaga
     8281 cacattggac aaaaggcaag tagtgcaacc tcaagtgtta ctaaggcagg gtcagcttta
     8341 gctattggaa aagcggcttc aaaaattttt cgtaaaaaag gagacaaaaa atgagtaaat
     8401 tatttaaaac tttagttatc agtgctgcat caggtgcagc agctgcttat tttttaacaa
     8461 caaaaaaagg aaaagagcta aggaaaaatg cagaaaaatt ctatggagaa tataaagaaa
     8521 atccagaaga atatcatcaa atagctaaag ataaagcaag tgaatattca aatttagctg
     8581 ttgatacttt taaagattat aaaggtaaat ttgaatcagg tgaattgaca acagaggata
     8641 tcgtctcagc cgttaaggaa aaaagcggag aagtagttga ctttgctaat gattttgtca
     8701 atcaagctaa atcaaaattc tcagacgagg atactgctaa aaaagaagat aaggctcctg
     8761 aaacaaaagt agaagatatt gtcattgatt ataaagaaaa cacagaagat aaagaaaaat
     8821 aaaaaagaag ctactataag tagcttcttt tttatgagat ttttattgtc tgagtgacca
     8881 aatgaatgaa gagactgcag atgtaactgc tagacttgcc agcatcgcta gtaagaaact
     8941 ccacacaggt gttaaactta gtgctagtaa taagaaagag attagcacag tggttatact
     9001 gaagagagcg atacgagcaa aaaagactag ctcacgcaac ttagcactgt gatcaaattc
     9061 ttgaaatact tgaaattttg gttgagcttg ctctgttttg ctttcgtgag tttgctcttg
     9121 cttaagtggt gatgacatag ctttctcctt tgatactagc atatcatatc acaaataata
     9181 gagtttgtca ctagtttttt gatactatga gttaaaataa aggttttttc tcaaaaaaac
     9241 aaaaacattg cccatattag tagtagaata ctaaaagtat tttttactac tttccttact
     9301 tttttaaatt ggaaaggaaa aaaatagata taatttcgta tagtatttca atactatgtt
     9361 tttttcttat ctgatagtgc ttaacctcta aagtgatata attttcgaaa agaatatttt
     9421 ttatttattc ttgtcaagaa atatgcttaa aaatgctgaa agtttgttat aatactaatt
     9481 atggaaaaaa taattttgac agcgacagct gaatctattg aacaagtaaa gcaattgcta
     9541 gcaataggaa ttgaccgcat ctacgtaggg gaagaaaatt acggtcttcg tttaccacat
     9601 tcattttcag acgatgaatt gcgtgaaatc gctaaattgg tacatgatgc tggtaaagaa
     9661 ttgacagtag cttgtaatgc actcatgcat caagaaatga tggataatat caaaccattt
     9721 ttggagttga tgaaagaaat taatgtggat tatttggtag ttggtgatgc aggagtattt
     9781 tatatcaaca agcgtgatgg ttataacttc aaacttatct acgacacttc tgtattcgtt
     9841 acatcaagtc gtcaagttaa tttttgggga caacatggag cagtagaaac ggttttagca
     9901 cgtgaaatac cttctgaaga actttttaag atgtctgaga accttgaatt tcctgctgag
     9961 attttagttt atggggcttc tgtaattcat cactctaagc gtccgttatt gcaaaactat
    10021 tataatttta ctcatatcac tgatgaaaaa acacgtgagc gtggtctttt tttagcagaa
    10081 ccaggtgatc cagaaagtca ctattctatt tacgaagata agcatgggac tcatattttt
    10141 attaacaatg atatcaatat gatgactaaa gtgactgaac tagttgaaca tcactttact
    10201 cactggaaat tagatggtat ttattgtcca ggagataatt ttgttgctat tgctgaaatt
    10261 tttgtcgaaa cagctcgttt gattgaaaat ggaacattca ctcaggatca ggctttcttg
    10321 tttgatgaac gaattagaaa actgcaccct aaaggacgtg gtttagatac tggattttat
    10381 gattttgacc catcaacagt aaaataagat ttatttgcta cgtgaaagtt ttttatctca
    10441 aagcgtagcc aattgcacta agaactgcat atcgattggt atgttaatta agatagaaaa
    10501 taattctttt atcttgaaaa tagaaggaaa tataagatgt ctaatgtaaa aaaacgccct
    10561 gaggttttat cacctgcagg aacactagaa aaattaaaag ttgctattga ttatggagca
    10621 gatgctgtat ttgttggagg tcaagcgtat ggtcttcgaa gtagagctgg taacttttct
    10681 atggaagagt tacaagaggg aataaactat gctcatgcga gagatgctaa agtttatgta
    10741 gcggctaata tggttactca tgaaggtaat gagcttgggg caggtccgtg gtttcgtgaa
    10801 ttacgtgata tgggactaga tgcagtcatt gtttcagatc ctgctcttat tgttatttgt
    10861 gctacagaag caccaggttt agaaattcat ttgtcaactc aagcctcttc cacgaactat
    10921 gaaacttttg aattttggaa agagatgggg cttactcgtg tcgtattagc acgtgaggtt
    10981 actatggcag agttggctga aatcaggaag aggacagatg ttgagataga agcatttgtt
    11041 catggcgcga tgtgtatttc atactcagga cgatgtgttc tatcaaacca tatgagccat
    11101 cgtgatgcta atcgtggcgg ttgctctcag tcatgtcgtt ggaaatatga cctctacgat
    11161 atgccatttg gacaagaacg tcaatcgtta aaaggcgaga ttccagaacc tttctcaatg
    11221 tcagctgtgg atatgtgtat gattgagcat attccagata tgattgaaaa tggcgtagat
    11281 agtttaaaaa tagaaggacg tatgaaatcc attcattatg tttctacagt aactaattgc
    11341 tataaagctg ctgtagatgc ctatatggaa agtccagaag cttttgaagc tattaaagaa
    11401 gacttgattg atgaactttg gaaggttgca caacgcgaat tagcaacagg tttttactac
    11461 catacaccaa ctgaaaacga acaactcttt ggagctcgtc gtaaaattcc tcaatacaaa
    11521 tttgttgggg aagtggtttc atttgacaat gctaaaatgg aggctacaat tcgtcagcgt
    11581 aatgttatta tggaaggaga tcgcatagaa ttctatggtc ctggcttccg tcactttgaa
    11641 tgttttattg aaggtctgcg cgatgctgaa ggaaataaaa tagaccgtgc tccaaatccg
    11701 atggaattat taaccataac attaccaaat ccagtaaaaa aaggggatat gattcgtgct
    11761 tgtaaagaag gattagtgaa cctttatcaa aatgatggta ctagcaagac tataagagct
    11821 tagatacttt tataactatt ttgtcatttt acatggtcaa tattactaaa aattgttaaa
    11881 atagaagaag ttcatttaaa tagaacaaaa agctataaaa ataacgaata gtataagtgg
    11941 aggtataaaa tgtcagaatt tacatttgaa attgttgaga agttattggt cctatctgaa
    12001 aatgaaaaag gttggacaaa agagttgaat cgtgttagtt tcaacggagc gccagctaaa
    12061 tttgatttgc gtacatggag tccagatcat acaaaaatgg gtaaaggtat cactttatct
    12121 aatgaagaat ttaaagtcat tttagatgct tttcgtaaat aaataaaaag tggtttcaaa
    12181 aaccgctttt tttgtcgaga caagcgagta ttcagtaaac atgttgatta aaaaaataga
    12241 atttttaaac taaaagaatt ataatggagg taaaaggagt cctctatgat tagacattca
    12301 tttaaaagta agatgttatt gttagtcttt atcttaacaa taatagtgaa ttatttatcc
    12361 gcaacaggtt tgttaacagg gaacagtcaa aagagtctgt ctgatcgtta tcaaacgctc
    12421 ttgacaccag caccattagc cttttcaatt tggtcagtca tctatttatt gaccttttta
    12481 gtaatattaa gagctatatt cagtaagagt caatcttacc aagataactt tgctagcatt
    12541 tttccatact ttttgggtct tctattagtt aataatatat ggactgtatt ttttacgagc
    12601 aatttgattg gcctatcaac aataataatt tttgcttact gtattcttct ggttatcatt
    12661 atcaagatac taagtaaaaa taaatcaaaa ttactgctta gaataacatt tggaattcat
    12721 gcaggatggt tgctagttgc gtcattggtc aatttagcgg tctacttagt taaaattgat
    12781 tttaattacc cattaccaaa ggtatatata gctatcattg ccttgatttt tataactgtt
    12841 ttaagtcttt acctggctag ggtattacaa aatgcttact taatcttatc cgttttttgg
    12901 gcttggttga tggtttttaa agcacattta gaaggaaatt ttcaacacct atattttagt
    12961 ttccaaattt tatctttgat tgcagcaatt atccttggcg tatcgttttt catcatcggt
    13021 tattataatg tcaaggagag gtacaagcaa aaacgtacta gtccttactc acgggttctt
    13081 taaaagaaat acaaaaagct ccatcattat gtactttcct ctatcattag atgtatgtct
    13141 catgatagag gttcagttca ttagtggggc ttttttgtat tgtattaaag taaatccatg
    13201 attaatttca aattgagtaa tgttaaaatg atagcgataa ggtatgctaa ataggtattc
    13261 catttagtat tagcatgctc tcccataatt gatttttgcg atgtgaaata aatcaatgga
    13321 aagattgaaa atggtaaagc taaggaaaga aaaacttgtg aatagacgat tagttgatct
    13381 aacgtatttt cttcgcctcc aactagtagg gcgatagcaa aaattggaag aagagttaat
    13441 aatctagtac aaagccttat taaccattga ggtaatttga agtgaaggaa accttccata
    13501 acgatttgtc ctgttaaagt acctgtaatt gttgaatttt gaccacttgc tagtagggca
    13561 actgcaaaaa gggtagaaag gaaagaactg gctactgcac cagtaatagt tttatcagat
    13621 aaggcaagat acatttgaga aaaggcactg atatcattag catgcccata aaatagacta
    13681 gctcctaaaa ctaatagtag cgaattcacg acaaatgcaa gtgatagctg aatgttagaa
    13741 tctaaggtca tgaaacgtac agcttttttt attgaagatt tattatgata atccacgcgt
    13801 ctagtctgag ataaagaaga atgtaaataa agattatgtg gcatgactgt tgcgccgata
    13861 atgccaagag cgagggttaa aggactattt ttcccttcat gtgatatgtt aaaaagctca
    13921 tggtgtggta aaaatccttt gaacatagca tctaaatcag gttgtgataa aacaactaaa
    13981 tatgtaaaga ttatcaaaat tgtcaatatg agaacagaaa caaaggcttc aattttttgg
    14041 acgcctaact tcattaagag taaaagtaag aatacatcca aaatagtaat catgatagac
    14101 agtaacaggg gccagccaaa taataagtga agagcaatgg cagagccaat gacttctgct
    14161 aaatcagtag cgataagggc taactcaatg ataataaata gcaggtaacg aagcggtttt
    14221 gggagtcgag aagctgttgc ttgtgctaag tcctgtcgtg taacaatacc aagcttgcca
    14281 gccatttgtt gtaattgcat cgccatcaaa gaagatacta aaacaacaaa caatagtaag
    14341 taacgatagg tagctccacc aataacgcta gtaatccagt tacctggatc catataacca
    14401 actgcaacca aagctccagg tccaagaaaa gctcttaaag tgttccaaaa agagctattg
    14461 tgaggaacct caacgctctg attaacttcg gataaggatc ttttgacgga atgattttga
    14521 ttcatgtaat actatacttt ctagtaatct caagccatta taacaaatag aaaatagaaa
    14581 aacaattaaa aattaatgct acttaataat cgattgaggt g
//
LOCUS       DATRME010000050        14445 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRME010000050 DATRME010000000
VERSION     DATRME010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            203..2275
                     /locus_tag="VBC23_001452"
     CDS             203..2275
                     /locus_tag="VBC23_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6843395.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(2312..2722)
                     /locus_tag="VBC23_001453"
     CDS             complement(2312..2722)
                     /locus_tag="VBC23_001453"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6843396.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(2792..4141)
                     /gene="gdhA"
                     /locus_tag="VBC23_001454"
     CDS             complement(2792..4141)
                     /gene="gdhA"
                     /locus_tag="VBC23_001454"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6843397.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            4309..4818
                     /locus_tag="VBC23_001455"
     CDS             4309..4818
                     /locus_tag="VBC23_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6843398.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(4859..6628)
                     /locus_tag="VBC23_001456"
     CDS             complement(4859..6628)
                     /locus_tag="VBC23_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843399.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(6633..8372)
                     /locus_tag="VBC23_001457"
     CDS             complement(6633..8372)
                     /locus_tag="VBC23_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843400.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(8439..8912)
                     /locus_tag="VBC23_001458"
                     /pseudo
     CDS             complement(8439..8912)
                     /locus_tag="VBC23_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(8909..10777)
                     /locus_tag="VBC23_001459"
     CDS             complement(8909..10777)
                     /locus_tag="VBC23_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6843401.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(10788..11996)
                     /locus_tag="VBC23_001460"
     CDS             complement(10788..11996)
                     /locus_tag="VBC23_001460"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6843402.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGSTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(12181..13029)
                     /locus_tag="VBC23_001461"
     CDS             complement(12181..13029)
                     /locus_tag="VBC23_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6843403.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(13029..13409)
                     /locus_tag="VBC23_001462"
     CDS             complement(13029..13409)
                     /locus_tag="VBC23_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6843404.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(13481..14014)
                     /locus_tag="VBC23_001463"
     CDS             complement(13481..14014)
                     /locus_tag="VBC23_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843405.1"
                     /translation="MTMKTIEEIYKNYLNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
     gene            complement(14065..>14445)
                     /locus_tag="VBC23_001464"
     CDS             complement(14065..>14445)
                     /locus_tag="VBC23_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO6843406.1"
                     /translation="YFKPTQIKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASH
                     LAAIYLLIHGGIKLVALILLHKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLC
                     VFDVILIILTWLEYQKMKLNFSKR"
BASE COUNT         4706 a         2866 c         2211 g         4662 t
ORIGIN      
        1 aagttaccgt gagaactatt tttacattaa tatttctaaa aaacacctat cattcgtcaa
       61 ataaaaaatg atttcgctta ctttttaata ttaaaaatat atgttcgctt tttatatttt
      121 taattataaa tttatattga tttttaatgt aaatgatagt aaaatacaaa tgtaatactt
      181 atttaattta caggagattt aaatgaaaaa gaaaattatt ttgaaaagta gtgtccttgg
      241 tttagtcgct gggacttcta ttatgttctc aagcgtattc gctgaccaag tcggcgtcca
      301 agttataggc gtcaatgact ttcatggtgc acttgacaat actggaacag caaatatgcc
      361 tgacggaaaa gttactaatg ctggcactgc tgctcaatta gatgcttata tggatgatgc
      421 tcaaaaagat ttcaaacaaa ctaaccctaa tggtgaaagc attagagttc aagctggtga
      481 tatggttgga gcaagtccag ctaactcagg gcttcttcaa gatgaaccaa ccgttaaaac
      541 atttaatgca atgaatgttg agtatggcac attaggtaac catgaatttg atgaaggttt
      601 ggcagaatac aatcgtatcg ttactggaaa ggcccctgct ccagattcta atataaataa
      661 tattacgaaa tcatacccac acgaagctgc aaaacaagaa attgtagtgg caaacgttat
      721 tgataaagtt aacaaacaaa tcccttacaa ttggaaacct tacactatta aaaatattcc
      781 tgtaaataac aaaagtgtga acgttggctt tatcggaatc gttaccaaag acatcccaaa
      841 ccttgtctta cgtaaaaatt atgaacaata tgaattttta gatgaagctg aaacaatcgt
      901 taaatacgcc aaagaattac aagctaaaaa tgtcaaggct attgtagtcc ttgctcatgt
      961 acctgcaaca agcaaggatg atattgctga aggtgaagca gcagaaatga tgaaaaaagt
     1021 caatcaactc ttccctgaaa atagcgtaga tattgtcttt gctggacaca atcatcaata
     1081 tacaaatggt cttgttggta aaactcgtat tgtacaagcg ctctctcaag gaaaagccta
     1141 tgctgatgta cgtggtgtcc tagatactga tacacaagat ttcattgaaa ccccttcagc
     1201 taaagtaatt gcagttgctc ctggtaaaaa aacaggtagt gccgatattc aagccattgt
     1261 tgaccaagct aatactatcg ttaaacaagt aacagaagct aaaattggta ctgccgaggt
     1321 aagtggcatg attacgcgtt ctgttgatca agataatgtt agtccggtag gcagcctcat
     1381 cacagaggct caactagcaa ttgctcgaaa aagctggcca gatatcgatt ttgccatgac
     1441 aaataatggt ggcattcgtg ctgacttact catcaaacca gatggaacaa tcacctgggg
     1501 agctgcacaa gcagttcaac cttttggtaa tatcttacaa gtcgtcgaaa ttactggtag
     1561 agatctttat aaagcactca acgaacaata cgaccaaaaa caaaatttct tccttcaaat
     1621 agctggtctg cgatacactt acacagataa taaagagggc ggggaagaaa caccatttaa
     1681 agttgtaaaa gcttataaat caaatggtga ggaaatcaat cctgatgcaa aatacaaatt
     1741 agttatcaat gactttttat tcggtggtgg tgatggcttt gcaagcttca gaaatgccaa
     1801 acttctagga gccattaatc ccgatacaga ggtatttatg gcctatatca ctgatttaga
     1861 aaaagctggt aaaaaagtga gcattccaaa taataaacct aaaatctatg tcactatgaa
     1921 gatggttaat gaaactatta cacaaaatga tggtacatat agcattatta agaaacttta
     1981 tttagatcga caaggaaata ttgtagcaca agagattgta tcagacactt taaaccaaac
     2041 aaaatcaaaa tctacaaaaa tcaaccctgt aactacaatt cacaaaaaac aattacacca
     2101 atttacagct attaacccta tgagaaatta tggcaaacca tcaaactcca ctactgtaaa
     2161 atcaaaacaa ttaccaaaaa caaactctga atatggacaa tcattcctta tgtctgtctt
     2221 tggtgttgga cttataggaa ttgctttaaa tacaaagaaa aaacatatga aataataaaa
     2281 ataggtttgg ataaaatatc caaacctatt tttatatcaa tatgccttcc aaatgatcta
     2341 actcgtgttg acaaatttga gctggaaaat ctgtcaactt gatttgctgt tctttccaat
     2401 tgatatcacg ataagaaatc gtaatgtgat gatatcgttg tgtacttctg caacctacga
     2461 gagaaaggca agattcttct gtctcatatg gatccgattt tgcaactaat acagggttaa
     2521 acattactaa atttgtaata cccacattga tgataatgac acgcttcaga ctaccaatca
     2581 tattggctgc catacctaca caataatttt gattagcatg caaagtatcc tgtaaatctt
     2641 ttgctaagct cacatctgct ctactagcca tttgtgattt ttgttgtaaa aagaaagtat
     2701 ctctaacaat tggttttatc atttatttta acctaactct attatgataa tactagtata
     2761 ccataggtta gctaaggact atgataagag attaaacaat accttgtgca gacatagctt
     2821 gagcaacttt taaaaagcct gcaatgttag cacctagaac caggttccca ctatcgccaa
     2881 attcatcagc cgctttggaa gcattcacaa aaatagtttt cataatacgt tgcaattctt
     2941 gatcaacttc ttcaaaagtc catgccctac gactagaatt ttgtgccatt tcaagcgctg
     3001 aaacagctac tcctcctgca ttggcagctt tagcaggacc aaaagcaacc cctgcctttt
     3061 ggaaaacttt aatagcaccc aaggtagatg gcatatttgc tccttctgtt actgccaaga
     3121 caccattttc aactaataac ttagcgtctt cttcgtctaa ttcgttttga gtagcacacg
     3181 ggaaagctaa atctgcttta atagaccaga tactaccttg atctgctggt gtgaaattag
     3241 cttttggatg tttctccgta tatttaacaa tacgtgctct ttcaacctct tttatctgtt
     3301 tcaatgtttc aaggtcaata ccgtctggat cataaacata acctgaagaa tctgacacag
     3361 ctacaacagt tgcacccaac tcttgtaatt tttcagtagc ataaatagcc acgttaccag
     3421 atcccgaaac cagtgcaact ttgccagtta aatcttgacc acgcgctgcc agcatttctt
     3481 tagcaaaata gactgcccca taaccagttg cttctgtacg agccaaagag ccaccataag
     3541 tcagtccttt tccggttaaa accccatttt gataaccatt taaacgctta tattgaccat
     3601 aaagataacc aatttcacgt ccacctactc caatatcgcc agctggcaca tctaaatctg
     3661 gtccaatgta tttttgaagt tcggtcataa aactttgtgt gaaacgcatc acttcattat
     3721 ctgatttccc ttttggatca aagttcgacc ctcctttgcc accaccaatt ggtagacctg
     3781 tcagtgaatt cttaaaaatt tgttcaaatc ctaaaaattt gacaatagat tgtgttactg
     3841 tagggtgaaa acgtaaacca cctttataag gaccaatagc tgatgaaaat tgtacacgat
     3901 atcctcggtt aacttgaact tggcctttat catctaccca tggaactcta aaagaaatca
     3961 cgcgctcagg ctcaaccaaa cgttctaaaa gattttcttc aatgtactga gggtatttat
     4021 caaaaactgg tactagtgac tcaaaaacct cttcaaccgc ttgtagaaat tcttcctcat
     4081 gctcattttg cttcttcact ttctctaaaa cacttgctac atatgtcttt ccgcttgtca
     4141 tttattttcc tccgtattgt tttaatatag ttagcattat aaattgtcag aattttcttg
     4201 tcaactattt gctatggtat cattattagg agcaatttta aaggtaatta tttgactttt
     4261 actgactttc gttctataat gagacaaata tttgattgga gtatctatat gaaatggttc
     4321 ccacttgttg gaggtataat tgccttatta gctggcttct atctctttat aaatcctgta
     4381 acggttgttg caactctggg ttggttcatt gctttattta tctttattta tggcatttca
     4441 aatttagtac gctacttgca agctgattct tatcaccgta gcccttggta tctggttcag
     4501 agtatccttg ctattatttt tggtatcatt ttactatcca gctcagcaat gtcactttca
     4561 aatactgtca tgactatttt agcatactgg ctactggtat ctggtatttt acgacttttg
     4621 ggtggtttcc aaatgcaaaa aattaatttc ccaggtgcca aatcctataa acgttcggct
     4681 tatattgcac tcattttagg tattatttta ctggttcaac ctaccttatc agcagccatc
     4741 attggaagat ttgtagcctt tatattaata gcaattggag cttcggctat ttacactttt
     4801 actcgtatta attattaaaa ggacatgttt cgtgtccttt ttttatctat tgatgacgtc
     4861 atttactatt tgaaaattgg ctattatata attcgtaata aaatccttta tctgctagga
     4921 gtttctggtg attaccttgt tcgatgattt gaccatcctt tagaactaaa atgttgtctg
     4981 cctcttgaat cgttgataga cggtgtgcaa tgacaaaact agttcttcct tccatcaatt
     5041 ttttcatagc tttttgaata agcaattcca accttgtatc aacagatgaa gtcgcttcat
     5101 ctaaaattaa aattttagga tctgctaata aagcacgtgc aatagtcaac agttgctttt
     5161 gtcctaagga gatattgctt gattcttgat tcatcaccat attgtagcca ccaggtaacg
     5221 ttcgaataaa gtggtcaaca ttcgcagctt tggcagcagc aactatatcc tcatcactgg
     5281 cttcaagatt accgaatcta agattttctt ttattgtacc ttcatataac caagcatctt
     5341 gcaacaccat gccaaattgt tgccggaaat cttggcgtga taggtgtcta atatcgtgac
     5401 catctacggt gatagcacct tcactaacat cgtagaatct catgagtaag tttataagtg
     5461 ttgtcttacc tgcacctgtt ggccctacaa ttgcaatcat ttgccctggt tctacatcta
     5521 agttaaagtc gcggataagt ggcttatccg gagaatagtg aaaatcgaca ccatgaaagc
     5581 tgacttgacc ggttaagtca tggctcaatt tttcagtgac ttgattggct tcctcttctt
     5641 catctaagac ttcaaaaata cgttctaagg aagattttgc actttgcaaa acacctgcta
     5701 attgtgtaat ggtttgaacg ggttgactaa tttgccaaac atactgcaca aatgcctgca
     5761 tgttaccaat tgttaaggta ccagcaataa cttgtaaacc gccaacaaat gcaatgataa
     5821 gataaataaa atcagagata ctattcaata ctggcatcat aattcctgaa atgaagctag
     5881 ctttgaaacc tacatgacgt agattatccg ttatatcacg aaattcttga ctagacgcct
     5941 cttctctacc atataatttt atgatattga aaccagttaa tttctcttgt acaaaaccat
     6001 tcagttctcc cagaatcttt gcctgttctt taaagtaagg ttgggatctt tttagtatcg
     6061 cttgtgcact aaaataagtt acaggaatac aagcaatgat aatcatggct aatggtacat
     6121 ttagataaag taccataacg actactagaa taatacttaa aaaagcattg ataatctgta
     6181 aaaagctctg ttgtaaagca tttgagaccg tttcaacatc acttgtaaaa cgtcccaaca
     6241 tatctccaaa ttggtgctta tcaaaatacg atacaggaac tttattgatt ttacgattca
     6301 aatcatgacg gatatcacgg atactttttt gaacgacagt agtcataaaa taatttgagc
     6361 cataagaacc taattcatag aaaacacctc tgaaaaaata aataatcaga ataccagcaa
     6421 tataaggaac gttcaattct gccccagaaa ctcccttagc catatcaaca aggttagcag
     6481 tcaactctgt tatcgctaac cctaaaataa aaggctccag aatactcata aaactagata
     6541 aaactttcaa aaaaatcgct aagaaaagag tagctttgta gcgagttagg taagaccata
     6601 atctcaaaaa aatagatttt tttctcatac aatcactctc cttctgataa gttttggttc
     6661 ttcagttgtg actcagcgat ttcacgataa atagcattat tttctattaa ttgagcgtgg
     6721 gtaccacgac cgacaatttc gccttcatca aggacaataa tctgatcagc atccataatc
     6781 gtacccaccc tttgagcaac tatcaaaacc gtagaatcac cggttacttc ttttagacgc
     6841 gctcttaaag tagcgtctgt cttataatcg agagcagaaa atgaatcatc aaaaatatat
     6901 aaatctggat ctttaacaac agccctagca atggataacc gttgtttttg acccccagaa
     6961 agattgctcc caccttcagc taaatgcgtt tcaaaggctt cttggtgact ctcaataaac
     7021 tctttagctt gagaaatatc aaccgcttgt ctaagatcat caatagtagc atcagctttt
     7081 ccatatttta aattctctcc tattgtccct gtaaataaaa gagctttttg ggggataaat
     7141 ccaatctttt ggcgaagtga tttaaggtta taatctctta catcaactcc atctactaag
     7201 atttttccaa gtgtcacatc ataaaaacgt ggaatcaaat taacaagaga agattttcct
     7261 gaacctgttg aaccaataaa agcaattgtt tctccaggct tagctttaaa agaaatatca
     7321 tgcaaaacgg gactctctgt ttctcctgga taagcgaatg ttacattatc aaattctaaa
     7381 tgccctttaa gtttcgtatc cgtaacacct tcggtattag gattaataga aatcggcata
     7441 tccattacct ctctaatacg atggctagat accaccatac gaggatacat agtaaaaaga
     7501 ttggcaaata gcaaaaatga aaagagagca tggaagctat attcgataaa agcaactaaa
     7561 tcccctattt taatagcacc tctttgtaag ggatccaaag caaaccaaac gatagccaca
     7621 atcattgcaa taataatttg aacgaaaagt ggttctgtta gccctgttaa tttaaaaaga
     7681 ccagttgaag tatctgtgta acgttggtta gcgacttgaa atttttgtga ttgaaaattc
     7741 tctcttgcaa aggccctaac aacgcgtaac cctgttaaat tttcacgaac atattgattg
     7801 attttatcaa gcatagtctg ttgtctttca gataaaggtt ttgtttttat agctacatat
     7861 aaaatgactc ctaccaaaag aggcatcgca accgctacaa gccaagccaa agatggactc
     7921 gtaattagta tcataaccac gctaaaaatc attaccatag gagttactag gcctaaacgt
     7981 aaagacattt cagcaaattg catcaaaaca aaagtatcgc ttgtcatacg tgtcactagt
     8041 gaagatacac ctatctgttc atattcatga tgggagtatt cttgaagctt agcatacata
     8101 tcattacgca tatctctaat cattgttgtc gttaagcgac tagatgcgta agtcatcgta
     8161 atacgcccaa taatacctag tacaacaaat ataaacatga tgaacgtcca cagataaact
     8221 ccagtccgat caccttttgt aacgccatta tcaatcatac ccgctaaggc tgtgggcatt
     8281 cctaggataa ccgtcacaaa aagcatagct cctagtaaat caagccataa ccaatttggg
     8341 taccttttca aataagacca aatatatgcc atatatgtct ccttttcttt cctcttatct
     8401 caaaataatt aatccatgtt cactaatcca ctttcaactt ataaatcttt aatcatccaa
     8461 atatccatag ctgtatgacc ttgatcattt gctattggtt gcgataaggc acaatatccc
     8521 atatgcttat aaaccgcagt tgccctactc aaaatactgg ctgtctctaa ataaagttgt
     8581 ctatacccaa tttttcgagc ttctacttca atcattttca ctaaatcagt agcaagcccc
     8641 ttaccacgga aacgttctgc aatgtacacc ttctgcatct ctgcaattag atttttcagc
     8701 ggaccaaagc cgcttcagcc aataatctca tctctctcct caatgacaaa gaatcctgac
     8761 ttctctattt tttcgtagta tgaggtcaag tgatctaagt gagggtctga atatgctgta
     8821 ccaggtttat ctaaatcata ggcgcgtaaa ctctgtcgaa ttaattgtgt aactgcttgg
     8881 tcatcatttt taataatcgg acggataatc atgtgtctaa cccactaagg tactcataac
     8941 ggtcatactt ttctagaagt gcttcatttt ttgcatctaa ttccttttgt aaatcagata
     9001 accttgtaaa atcactacca catgtttgca tatcatcttc tatacgagtg atagtatttt
     9061 ccaatatcat catatcgtct tcaattgtcg cccattcttg tttttcaaag taagacattc
     9121 ttttacgact ttgcttttca cgacttgtct tggtactctc ttttttactg ataacttcat
     9181 tattttgctc attaaatgct ttttcatcta aataatcagt ataattacca aaaaattcac
     9241 ggatatcgtt atcttcaaat gcaataattt tattagccac tttatctaaa aagtaacgat
     9301 cgtgactaac tgtaatcaca ggaccaccaa agccttgtaa aaaattttca agaacagtta
     9361 atgtagcaat atctaagtca tttgtcggct catcaagtag taacacgtta ggcttttcaa
     9421 tcaggatttt taaaaggtaa agtcgttttt tctcaccacc tgataatttt gcaatttgtg
     9481 ttccatgtgt cgaacgtgga aagagaaatt gttccaatag ttctgtcaca cttgttgtac
     9541 cgacactagt tttaacctca tctgcaacct cttgcaaata attaataaca cgttttgagc
     9601 catccatatt atgaagttgt tgtgaaaagt aacctacacg tatcgtttca ccaatagaga
     9661 cattacccga atccggctgt aaatcttgaa caattaaatt aagtaaggtt gactttccaa
     9721 caccattatc tccaacgatg ccaatacggt ctttattttg aattaacaaa ttaaagtctt
     9781 tcaagataga tttatctggg taagaaaaag agacattttc aaaattaata acctttttcc
     9841 caattcgact tgtttcaaat gtcatttcca aatcgcttgt atcgcttgtt tggtgtaaat
     9901 cgttttttag attttgaaaa cgattaatac gagcctgttg tttcgttgca cgagcttgtg
     9961 gctgagtacg catccaagct agttcctgtt tataaagctg tttcttttta tgtaaactag
    10021 cagcatcacg ctcgtcttgt tctgcacgaa gtcggacata atcctgataa ttgccttgat
    10081 attctgtaat ctgtgcctta tctaattcaa aaatacgtgt tgcaacattg tctagaaaat
    10141 aacgatcatg agttataaaa agcactgtct ttttactatt tttcaaaaaa ttcgttaacc
    10201 atgcaatagt gtcaatatct aagtggttag taggttcgtc taagagcaat aaatctgcat
    10261 catttaataa tacttgcgct aattgaacac gtcttcgtaa tcctcctgat aattcaccaa
    10321 ccgacaactg caaatcagta atacctaatt tggataatac tgttttgact tcgctctcaa
    10381 tagaccaagc atctaaagaa tccatttctg ccattacttt ctctagacgt gattgcttac
    10441 tttcttcgta gtgattaagc aataattcat attctttaat taaagccatc tctcttaagt
    10501 cagaagaaag tacggtgtcc aaaattgtct gagaatcatc aaagtctggt tcttgtttta
    10561 aataagcaat cttataatca ttagctgatg aaaaagggga acgatcacca tcaaaaccta
    10621 attcacccga aataacatct aatagtgttg tctttccagt tccattgaca ccaataatcc
    10681 caatacggtc taaactatgg atgataaatg aaacattact aaagaccgtc ttatcaccaa
    10741 ccgacttagt caatccatct actaaaaaat cgctcatata atcctctcta atccatcaag
    10801 tactgcttaa caaaatgtaa aatagcctct tcttcattaa tcagttgtcc taaaacaatg
    10861 gctaattcaa tttgagagat aatatctccc atctgtggac caggttttat tcctaatttc
    10921 ttgattaagg tgctaccatt gacaactatc tcatgcctat catggatagc taactcacta
    10981 tctagttgtt ctattttttc gaaatcaacc atcaagcctc ttgctttgcg aataccttcc
    11041 gcttgtttta ttatagtttt tccataacga taaagatgtt ctttagtcaa tcccatttgt
    11101 tcacgaatac gataaacatc cactatatgc tcaacatctt tttgaaagtg agttgatgtc
    11161 ttccattttt ttaaaaaagt ctttgtgtgt tcaacttcca tactcaagat gagtgatgcc
    11221 caagcttgtt ctgatgtctc aaataaaaaa tctttatcta actgggacaa aaaggcgctt
    11281 aattgtaatt ccctattttt taagcatggt agataattaa aagcatgact atctattagt
    11341 gccagcattc ctttacgcca ataaggtgct aatagaagtt tatcaaattc aataaaagaa
    11401 cgctcaacag atattttctc caataatggc gcgtgctttt tcattgcctc aaaagttgtt
    11461 gtttcgatat caaaatttag actagcagaa aaacgcaaac ctcgcataat tcgaagagca
    11521 tcttcattaa aacgctcact ggcgagtcct actgctctaa gaaggtgatt atctaaatca
    11581 tccaatccat gaaagagatc aataacttct ccatcttcat ttaaagcaaa ggcattaact
    11641 gtgaaatctc gacgttttag atcttcttct agcgacctaa caaaattgac ctgactcgga
    11701 cgcctataat caacataaac ctcctcagtc ctaaaagtgg taatctcata ttcaccacct
    11761 ttttccaata ctaagactgt tccatgttca ataccaacat caactgtcct tttaaaaatc
    11821 tgctttgttt cttctggata agatgacgtt gctatatcaa catcatgaat tggacggtca
    11881 agtaaaacat cccgaacact accacctaca aagtatgctt cataaccggc tttttttatc
    11941 ttttttaata ttggtaaagc cttctgaaac tcagaaggca aataatttaa tctcatcata
    12001 ctattccaaa tctaaacgaa ctaaagtcgc attattattt cttataaatg gattcagaaa
    12061 caaaaagaac tgcctaaggg acactcccta cctactcaaa ttatcaaaaa agaatctcct
    12121 aacttcttct ttgatatctg caataaggat caacctccaa gcaactcttt ttatcacaca
    12181 tcaatttttt ggggtaatac caaaggcaac cgcaccttca ccaagatgag ttgcaataac
    12241 gccaccaaat gatacaattt ctaaatcgtc tttaagacca gctttagcta acaaattata
    12301 taattgttct gccttatctt gtgctctaga gtgaataata gcaatatcat attcaccatc
    12361 tgctgtcatt tctttgacaa tctcagccaa gcgttttaag gcttttttct ctgtacgcac
    12421 tttctcatag acaacgattt taccttcttc attaaaatgt aagaccggct tgatacttaa
    12481 aaggttacca atgatagctg aaccgtttga caatctacca ccttttacta gatgattcaa
    12541 atcattaaca acaataaatc cttcaatttt ttcaatttgt gattgtatct tgttaactat
    12601 gacatcaaaa tccatccctt cacgtgaaca catcaaagca tttctaacta aattgccctg
    12661 tggtgctgaa gttattttag tatccggaaa agcaattgtc aaatttggat gttcttcaat
    12721 aagaaactga atattctgcc agaaacctga tatccccgct gcaatgaata aaccaaggac
    12781 gtgtgtatac ccttctttct ccaattgaca taagagatca tctagttcag ctaagctagg
    12841 ctgacttgtt ttaggtaatt cttttgatgc cgctagctta tcataatatt ggtctaatgt
    12901 cagattttgt ccctcaatat aagttttacc atcaatgata attggaatat ctaaaacata
    12961 gacatcttca tgattttcga tagcctcagg caagtaagct gaggtatccg taataagtgc
    13021 taatttcatt aactattaaa ctccaatgtt actcctggtc tatctaaagc aatttctgta
    13081 acttcataag tcagacgttt aacgatttct aaaagaggag ctgctagatt ctcaacttca
    13141 tcttgactaa actcactagg ctcattgata aggcgccctt ggatatgagc tatttgtgaa
    13201 atgacaccag aaataacaaa ctcatcacgt acaataacaa attgtaacac tgctacaatg
    13261 ctcgttactt ttgctgcttc atctctatta gctaattgga aagtaacttc aaaatttgtt
    13321 tttggggtac cattttcctc ttcccattct aaattgcgcg catcatagtg gtattgattg
    13381 acaaattctt gttcacgaat gacttccatg tattttctcc ttgatagaca atatgtatta
    13441 ttatagcata aatttgtaga ttcttaaagc aggtgggaat ctagaacttc ttcttaggat
    13501 gtctatcaat cacttcttga ttgttactca aggctgctaa acctttttct gtaagtgcca
    13561 accctctgac aacaaacaac ttccctaatt cctcttctgt aaaagcaata gctattgcct
    13621 gatctaaatc agagcgtttc atctttgaca agccttttac gtttttttct tttaagaata
    13681 gttttaaaag tggagcagat aagtgatcta aagaatcaag cggtgactcc attcttacaa
    13741 atccattttt tattaaaaat tctaggtgtt cttttccatt tataccataa atgtattcaa
    13801 aatattttga aaaacttgtt tcagttgtga aagttccaaa ctgtatacgc cataataaga
    13861 taatatctcc aggcaagagt ccttcactag tttcttccat atttttccta ggaacaactt
    13921 tactaaaatt aatttcagcc aagtctcttt caggtgagat ataggggata ttaagataat
    13981 ttttgtaaat ttcttcaata gttttcatag tcatattgta gcaaaaaagc gcttataaaa
    14041 acaagcgcta aggcatgggg gaagtcacct ttttgaaaaa ttcaatttca ttttctgata
    14101 ttctaaccat gttaaaatta tcaagataac atcaaataca catagaaaaa tcattaaaat
    14161 gctatgacta gaaaaatatt cccttagttg gaaaatgata aacccaacga aaactaaaat
    14221 tgataaaggg taagcaaata atttctcttt atgtaataaa ataagtgcta ctaatttgat
    14281 accgccatga attaatagat agatagcggc taaatgacta gccgaatcat taatataact
    14341 gcttattttc aagaaatgag cagaaataaa accatgaatc ccaccgtgaa cttcaccttc
    14401 agttaaaaga aataggagat gttttatttg tgtaggttta aaata
//
LOCUS       DATRME010000051        13595 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRME010000051 DATRME010000000
VERSION     DATRME010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13595
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..343)
                     /locus_tag="VBC23_001465"
     CDS             complement(<1..343)
                     /locus_tag="VBC23_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843407.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDT"
     gene            complement(347..2632)
                     /locus_tag="VBC23_001466"
     CDS             complement(347..2632)
                     /locus_tag="VBC23_001466"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6843408.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            complement(2656..3315)
                     /locus_tag="VBC23_001467"
     CDS             complement(2656..3315)
                     /locus_tag="VBC23_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6843409.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            3471..4673
                     /locus_tag="VBC23_001468"
     CDS             3471..4673
                     /locus_tag="VBC23_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843410.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            4691..5143
                     /locus_tag="VBC23_001469"
     CDS             4691..5143
                     /locus_tag="VBC23_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6843411.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            5311..6159
                     /locus_tag="VBC23_001470"
     CDS             5311..6159
                     /locus_tag="VBC23_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6843412.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(6238..7134)
                     /locus_tag="VBC23_001471"
     CDS             complement(6238..7134)
                     /locus_tag="VBC23_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6843413.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            7259..8014
                     /locus_tag="VBC23_001472"
     CDS             7259..8014
                     /locus_tag="VBC23_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6843414.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            8011..8940
                     /locus_tag="VBC23_001473"
     CDS             8011..8940
                     /locus_tag="VBC23_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6843415.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(8975..9348)
                     /locus_tag="VBC23_001474"
                     /pseudo
     CDS             complement(8975..9348)
                     /locus_tag="VBC23_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(9449..11761)
                     /gene="pflB"
                     /locus_tag="VBC23_001475"
     CDS             complement(9449..11761)
                     /gene="pflB"
                     /locus_tag="VBC23_001475"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6843416.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            11931..13040
                     /gene="dinB"
                     /locus_tag="VBC23_001476"
     CDS             11931..13040
                     /gene="dinB"
                     /locus_tag="VBC23_001476"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6843417.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            complement(13111..13593)
                     /locus_tag="VBC23_001477"
     CDS             complement(13111..13593)
                     /locus_tag="VBC23_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843418.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
BASE COUNT         4258 a         2232 c         2633 g         4472 t
ORIGIN      
        1 gggtatcgtt tagagcttcg agttgagttt ggaggagact cttccaatta tttatagcta
       61 ttaaattgag tgcaaagtca aagttcttta aattaacctg aatatagaat tttcgattta
      121 aatgagcaat cataaggatg gaagtatcag tagtattgcc aaaataccag aaataattcg
      181 aacttccgga accttgtaaa tcatcaacaa tagttgcaac aacctgtgga atgtttttat
      241 tatcatggtc aaaaactgct ccgaagttac tctcttgtaa taaattgatc tggtaccaaa
      301 agtgttgaga atcatcaaac cgttctttag tgagaagttt cataagttaa tttccttgat
      361 tagttgggat agttaaataa gattgactta aatctactgt tatagaataa ctagcattat
      421 ctcgaatggt atgttcaaaa tcagttgtat aaaggataat ccttagatta tcacctttac
      481 tcaattgata tatacttggt tggaggctaa actcgatatc aaaccattgt tctggctcga
      541 tagcctcaat tgtaagtaag tctgtacgat tttgaaggtt caagacacct ttactgatga
      601 cacgataatg atctttagta aacgggagtt cacgtaaagc ttctctggca aaatttttac
      661 cattatcaag gctgtttaag aatgtgatgg ttggtgtatc tttgaatcgt tttttaggac
      721 catagtctaa gatttgggct gataaaatgg cctttttgtc actagtttta acacgtagat
      781 ggagtttgca ctgtccattc aggagataat ttttctttag aggaagatta atagtgattt
      841 gattggtttt attatttccc ttgaaaagat catttttgaa cacattgaag tctttacaat
      901 aagtatcaaa ggcttctttg tcataatggt tatcaataag ttttttacta tcacctaaac
      961 caatttgctc ttgatggttt cctccgaaag catctaaaac ttgccaagtt tgctcagtag
     1021 tattatcttg ccaaatgacc tcttctaatt ggaaatgatt gtcaatgcca agtagttctt
     1081 ggcttagtaa ggcattcatg ctttcacgaa aatcaatcga ctgccaatta tgcatataca
     1141 catgttgacc ttgatgtaaa aaaaggtgtt ttttgatggt ttgaggcaat gcattgaaaa
     1201 ctttatagac atgtcttggc ttaacattcc aatcctgtag tccatgagtg taaactactc
     1261 gacttttgac attattgacg tgagttaggt aattacggtc atgccagtat tggttgtaat
     1321 ccccactttg acggtcaatt gcttttgatt gttcatttaa caatgcttga tagcaatcgt
     1381 tgtttttgat gtaatcacca gctaagaggt ttcgtgagta tgttaattct gttaaaacgt
     1441 ctaaatcttc accggggtag ccgcctggac tacacacaag cccattttct cgataataat
     1501 cataccatgt ggagattgcg gcttcagcga taatgacttt cagcccctca acgccagttg
     1561 ttgctaaacc agttgacatg gtaccaagat aagatttacc tgtggttgct acaaggccat
     1621 ttgaccaatt agccttgact tgtttatctc gtttatgact tgtgaatgca gtaaccttac
     1681 catttaacca atcaatgact gctttaaagc tttgtatttg ttggtaatcc ccactggtca
     1741 tgaaacccgt agagccagca gtacccacac ctgaaacgta tatattagca aaaccccgag
     1801 aaagaaaata gtcattgaga ctataactgt caatgtgtgt gaagctttca gttgcagggc
     1861 tgatgggtaa ttttgaagga tgttttactt cttctttaaa tggttttgtg tcaacttgta
     1921 tgtgtttagg ttgtttaact gctagcgcac cctccatttg gtatgttttt tggtctgcgg
     1981 caggatcatt gataccttga tgataggggc tagctgtcat catcgtaggg atagtatgct
     2041 cggtagtggg tctgataatg ttaactttta ctaaatcatg ttcaccacta gacatagtat
     2101 ctaaggatgt ttcaacatag acaacttcgc gaatcaattg gttagtattg aaagtggcca
     2161 gagacttccc attgaaaaaa tggtagtgat tatctgcatg aagtaaacca tcagcaacta
     2221 attggtcgat taatgtattg ccagattttc cacgacaggc gagtaagtga tgtagacttt
     2281 gaaaaatatt gtcatatgag actgggaagt taatatcttg taagaatgct ttagggtctt
     2341 taaagtctac aaaagggata aagcctaata actgtaaata aatccattgt aaattttcta
     2401 gtgttaagtc actatttgat ttaaaaaagg ttagagcatc ggtttttgca tcaacgataa
     2461 ggagactaag agcgtaatca gtatcagttt gatttaaaaa agaatgtctg agaaaagctt
     2521 ccaaattagc tttatcagaa ttctttttgt ttaatggaaa acctagtcct ttgagctctt
     2581 caaaagcttc gttaggttta gttggaatgt agctgaattg attataacgc ataatatatc
     2641 ctttctattg ttatattaaa atacaatatg atcttttaat ttctcaacat ttgtaataat
     2701 gatatgttgt ttttcaatac gaataatatg atcgtctttt agttgtttca aaatgcggct
     2761 cacgctactt cctgtggata ttccgcaaaa tttaccaagt tcttcgttcg tgataacaaa
     2821 attaatatag atatcaccgt tatctcgttc ttcaccaaac atcttactga ggtcatataa
     2881 ttgtgtggag atagcaccaa ttcgaccatt agtgagcata cattgcattt ttttcataga
     2941 tttttcaagg cggttatgat agaaatcttt cacatatcct tgcagttcaa tatcattatt
     3001 gatatctttt aggaaggtag agcgacgtac tttgtaaaag tgggcagttt cggattcgat
     3061 tcgaacgttg taaggttctc ccatgtgttg ggagtagtct gtatttagga tagaagtgat
     3121 ttctaagcct gtcacataac gcagattgaa ctcagtacca tatttagaga gaacactttg
     3181 tttaacaata ccatctttta ggatataagt ataatcttct tctaaacttt caaatgtaag
     3241 gtatttatgg tagtcttttt cgataattgg aagattatga ctattaatgt aattttgtaa
     3301 aatttgatga ttcatgactt cctccaaatg ttttaggttt gatagaccac cttaacattt
     3361 gttaatgcag tctgatacta gcacttatat tataccattg aaatatcgta tcttctaata
     3421 tttttgtaaa atgtttatga aaatgaaatt gaaaaaagga gccatttgca ttggaagaca
     3481 aattattcaa caaacatttt ataggcatta ctattttaaa ctttattgtt tatatggtct
     3541 attatttgtt taccgttatc atagctttta ttgcgactaa agagttaggt gttagcacta
     3601 gccaagcagg attagcaacg gggatttata ttgtagggac tttgattgct cgtcttatat
     3661 ttggtaagca attagaagtt ctaggacgta agttagtttt acgtggaggg gctatttttt
     3721 acttactaac aactttagct tatttttata tgccaagtat cggagtaatg tatttagttc
     3781 gtttcctaaa tggttttggt tatggcgtcg tgtcaacagc aactaatact attgtaacag
     3841 cctatatacc agctgataaa agaggtgagg ggattaactt ttacggtcta tcaacaagtt
     3901 tagccgcagc tattggtcct tttgtaggaa catttatgct agacaacctt catattaact
     3961 ttaaaatggt tattgtatta tgtagtattt taattgcgat tgtagtgttg ggagcatttg
     4021 ttttcccagt caaaaatatt actttaaatc cagaacagtt agctaaatca aaatcatgga
     4081 ctattgatag tttcattgag aaaaaagcaa tttttatcac aattattgca tttttgatgg
     4141 gtatctccta tgcttccgtg ttaggtttcc aaaaattata tacaacagaa attaatttga
     4201 tgacagtagg agcttatttc tttattgttt atgcacttgt catcacttta accagaccat
     4261 ctatgggaag attaatggac gctaagggag ataagtgggt gctttatcca agttatctgt
     4321 tcttaacttt gggacttgct ttattaggga gtgctatggg aagtgttacc taccttctat
     4381 caggtgcttt gattggtttt ggttatggca cctttatgtc ttgtggccaa gcagcatcaa
     4441 tcaaaggtgt tgaggaacat cgtttcaata cagccatgtc aacttacatg ataggtcttg
     4501 atttagggtt aggtgctgga ccttacattt tgggacttgt taaagatggt tttcttggag
     4561 ctggtgtgca atcctttaga gaattattct ggatagcagc gattattcct gttgtttgtg
     4621 gtattctata tttcttaaaa tcatctagac aagttgaaac taaaactata taaaagggag
     4681 gaattaagct atgacacaaa aatatgagcg tattcttatt gctattgatg ggtcttacga
     4741 gtcagaattg gcggtagaaa aagggataaa tgttgctctt cgtaatgatg ctgaattact
     4801 gctaacgcat gttatagatg ctcacgctta ccaaagtgag ggagtcttta gtgactatgt
     4861 ttttgatagg caagaacaag agtcagcaga tgtgctagcc tattttgaga aattagcaca
     4921 ttcaaaaggc cttacaaaga ttaaaaaaat tactgaaatc ggaaatccta agactctttt
     4981 ggcaaaggat atccccataa gggaaaaagc agatttaatt atggtaggcg caacaggatt
     5041 aaacactttt gaaagcctat tgatcggctc aacttcagaa tacattttac gtcattcgaa
     5101 agttgatatg ctagtcgttc gtgatagtaa gaaaacctta taaactaaat aaaatagagt
     5161 actaggtagt ccgaacctag tactctattt tttatcaaat atatttggtt aaaaatttta
     5221 ttaattttat tttgtgataa gctttacaat attaagctta cttggtagaa tggttaatgt
     5281 tgtaaatatt ttttggggag gaaacaagga ttgatcgaaa tcacttggac tgtgaagtat
     5341 atcacagaat ttatcgcaac cgcttttctt atcatcttag gaaatggggc tgtagctaac
     5401 gttgacttaa aaggcacaaa aggaaataat tcaggttgga ttattattgc aattggttat
     5461 ggtttaggtg ttatgatgcc agcgcttatg tttggtaatg tctctggaaa ccatattaac
     5521 ccagctttca cacttggatt ggcattttca ggtcttttcc cttgggcaca cgttggacaa
     5581 tacattcttg cacaaatttt aggagcaata tttggacagt tagttgtcgt tatggtttat
     5641 caaccttact ttgttaagac agaaaatcca aatcatgttc ttggatcatt ttcaactatt
     5701 tctgcattag atgatggaca gaaatcaagt cgtaaagcgg cttacattaa tggtttctta
     5761 aatgaattcg ttggttcttt tgttcttttc tttggagctt tggctttaac gaagaactat
     5821 tttggtgtag aacttgttgg taaacttgtt caagctggtt atgaccaaac gacagcagcg
     5881 acaaggattt caccatacgt aactggttca cttgctgtag ctcacttagg tattggtttc
     5941 ttggtaatga ctctagttgc ttctttaggt ggaccgactg gacctgcatt gaaccctgcg
     6001 cgtgaccttg gtccacgtat tgttcaccgc ttattaccaa aacaaattct tggacaagct
     6061 aaagaagatt caaaatggtg gtatgcatgg gtgccagttt tagcaccaat cgtagcaagt
     6121 atactcgctg tagccttgtt caaactatta tatctttaat agttaaagaa agatcggttt
     6181 accggtcttt ttttgtttta aatataaaaa tcagccagtc ttgctagctg atttcgttta
     6241 atgatgactt ttgggcatag tttttttaaa ggaagaacgt attaacattg ctccagtaaa
     6301 taaaacaata cctacaacca gtgttttcaa gctgttaaga tcaaggttag ttaagaagag
     6361 aacagcgaca ataacgccta atattccccc agtaaccaga ccggcaaagc cgttccaatt
     6421 aacacgacca ctcttgataa attgtgtact agaagctgtc attatcatag ctgcatctag
     6481 catcatgaca ggcatagcaa ctgcaggact cagtcccatt agggagaaga agataagttc
     6541 gggagcataa ttacctagtc ccatagtcat taatacacca ataataaaat tgaagacgat
     6601 accaacaaat aaccaaatgc cgtgtagacc atgcactgaa tctgaaatac cagcgccagg
     6661 gttagtgatc atacgataga gcataataat agcagctgtg atgagcagtg ttcctagaat
     6721 acgttgaaca gtaggagcgt gccaattttt agtcatttta gctccaacaa aagccccaat
     6781 aaaagctgca ccagccattg ttattagtgt taaagcctct acttttacaa caaaaataaa
     6841 acagagtgac tgcaacaaaa ctggaagtac gtgtgcagct gtcattgttg cgggtatctt
     6901 acgatcgtct tcaacaagct tagttagttt aaaaaatgtg gtcgttgttg caaaagagcc
     6961 gataccaaga gtatcgagag catcagttac aaaaccgatc cacaaaccag tgaaaaatcg
     7021 tttgaaagga tttatttttt gagacctaat atgtctaaca atctgataaa aagtgataat
     7081 catggcgata atcagaataa gctgaatccc atgtaatagt agtgtttcat tcataatatc
     7141 cagtctacaa aaaaatagtg aaactgtcaa tttgacttta tggtattgag agcacaaaga
     7201 ttcttgctaa attatctaaa attatctaaa atagactaga ctgaacaagg aggaatttat
     7261 gacgttattt cattcattga catttaaaca cccagtatta cgtgttaata atagagactt
     7321 gaatattgca ttttatcagg agtctcttgg atttaaattg ataagtgaag aaaatgcaat
     7381 cgcagttttc tcagcatggc agaacaaaga ggctagcttt attattgagg aatccccaac
     7441 ttatcgtact cgtgcggtga atggtactaa gaagttagca aagattattg ttaagtctca
     7501 agatgcaaaa gatattgaaa agcttttggc aaatggtgcg caagccattc aagtatatca
     7561 aggtcaaaat ggttatgcct atgaaacagt ttcgcccgag ggagaccttt tcttacttca
     7621 tgctgaagat gatttgtcac aattagtggc aattgagcgc ccagagttag aaaaaaaaga
     7681 tgacactaca ggtctatcaa actttgcttt ccaatctatt agtttgaatg tacctgatgc
     7741 cgtgaaagct gaagcttttt acgataaagt atttgctggg aagttcccaa ttaatttatc
     7801 tttcaaagaa gcacagggac aagatttgca aattgctcca aatgaaactt gggatattga
     7861 gatactagaa tgctgtgtta atgaagatac taatttaaat gacttgaaat caacttttga
     7921 atcatgggga cttgatgttt accttgattc taaagaaaag attttagtca tctctgacac
     7981 tagtaatatt gagatttgga tttcaaaaga atgattaaaa ctaataccgt tttagaactc
     8041 attaaggagc aaatcaaaga taatctttat catggtgcaa gcttagctat ttatgaaaat
     8101 ggagagtggc acgaacatta cttggggaca attgatggca atgagaaagt taaagcggga
     8161 ttggtctatg atctagccag tgtatcaaaa gttgtcggcg tgggaacttt actagcaaag
     8221 ttagtttatc aagggactat tgatattgat aagccattaa ggtattatta ccctacgttt
     8281 catcatcaga cgttaactgt aaggcaatta gctacacata gcagtggaat tgaccctttt
     8341 attccaaatc gtgatcagtt gaatgctact cagctgaaag atgccattaa tcatatcaag
     8401 gttttggaag ataaatcgtt taaatacact gatattaatt ttcttttact tggttttatg
     8461 ttagaagagg tattagggga tagtttggat aaacttttca aacgttatat tttcactcct
     8521 tttcagatga aagaaacctc ttttggtcct agagtagaag ctgttccaac tgtagccggt
     8581 attaacgatg gtattgtaca cgatcctaaa gctaaggtat taggaaagca tactggttct
     8641 gcaggtttat tttcaactat tgatgattta caaagatttt ctattcatta tttaaaagat
     8701 gattttgcga agcctttgtg gaataattat agtttgagta aatcaagatc attagcttgg
     8761 gatatagata aggattggat aaaccatact ggttatactg gtccttttat tgcactaaat
     8821 tatcaaaagc aggcagcagc tatttttttg acaaacagaa ccttttccta tgatgaccgt
     8881 ccactttgga ttaagaaacg acgacatgta caagaggcga taacatcgta ctttgaataa
     8941 aaaacttctc ctagaatttt ctaggagaag ttttttataa aagttggata acatttatgg
     9001 gagttacttc caatacttta cgcagaaaag ggtatttttc tttaacaatt tcaaaatcgc
     9061 ttcctgcttc taatagctta gcagttcctt caattctaaa gcctgtacct tgataaccat
     9121 cacgaccctc gacttcacgg ctgcccagag tcataataat tttagaatta ttattgagat
     9181 catttcaaga tgagtcattc ctgctgcagg agctaatata cgctggtcat ctgtaatagt
     9241 caggtagcta ttccaagtgt ttgtaacgtg aggagctaat tcggtccagc ttgttatcga
     9301 aacgacaccc tcatatttta gaacttgaag aaatttgtga tttaacatta ggcccctcct
     9361 tgaggttata taaagaagta attagtacta ttgataatat agataaaagt aggtttgata
     9421 taatcaaacc tacttcatcg aatacaaact agttagttaa tgcatcgtcc attgaaagta
     9481 cttcatggaa gacacgttgt gttaactcag ttttttgctc aggagttaga tatttagtat
     9541 tgacacagta acctgagata cgaacgataa catcttcacc attcataatc ttgtcataga
     9601 catctttaag gtccatgaca ttcaagttaa cgtgttgacc accgttttca aagtaaccat
     9661 caaggactgt taccaagtta tcaacttgtt catcaaatgt tttaccaaga gcgcgtggag
     9721 aaacttgagt tgtcaatgaa ataccatcat ttgcatgagc gaaatcaagt ttagaaagtg
     9781 aattcaagtt ttgtaaccag ccacctttag ctttgttaga agggttagca cctggtgaga
     9841 agaattctac tttagaaagg tttacactac catcttcatt gagatatact cctttatgta
     9901 ccggtgagtt accagtttgt ttagagtaag caacgtttga tgtgattgta agaagagaaa
     9961 cagttgcttc agcatcttta taaagtttgt gtttagcaag gcgtccatgg aaggcttcaa
    10021 gtaaccattc agcgattgag tcaacacgat catcatcttc accataacgt gggaagtcac
    10081 caactgtttc gtagtcgtag atgtaaccat cttcgtcacg gattggttta acagtagcgt
    10141 atttaatcgc agataatgaa tcgacagtat ttgcaaaacc acagatacca aatcccatat
    10201 tagcacgtac atgtgaaggt aagaatgcca tttgtacagc ttcatagttg tatttatcag
    10261 tcatgtagtg gatgatattc atcgcatcaa cataagtatc agtcaaccag tcaagagatt
    10321 tttcaaagtt agcttttaca gtgtcaaagt tgagtacttc gtcacgaatt gggtcgatgt
    10381 caaatacttt gtaatcttta tgaacatcat cataaccacc gttaagacca gtaagaagtg
    10441 ctttcataac gttaacacga gcaccaaaat attgaaggtt gtgacgtttg tcttcgtttt
    10501 ctggatcaag tggagataca caacatgaaa tacatgacat ttcaccgtag ccttctttag
    10561 ccattgttga tacaccttca tattggattg aagagtgttt atgactcatt gacatacagt
    10621 agcgacggaa agcatatggt aattgatctg accaaagaac agttaagttt ggttctggtg
    10681 agttaccgat attgtcaagt gtatttaaga aacgatagtc cattttggta acacgatgac
    10741 gtccatcagc tcccatacct gccattgaag ttgtgataaa ggttgggtca cctgagtaaa
    10801 gggcatcata agctttagta cgagcaaatt ttacagtacg gagtttaagg acaaaatcat
    10861 caacaaattc ttggatttct gattctgtga atgtaccgcg agcgaggtca cgttctgcaa
    10921 agatatcaag gacaatagga acacgtccaa gtgatgttgc agcaccattg ataacacgac
    10981 atacagccat gaaagcaatg ttcacccatt gaatggcttc tttagtgttc atggctggtt
    11041 tacgaacatc tacgccgtaa aggtcaccaa gttttacaac ttcaccaaga gcttggtatt
    11101 gaaggttgat ttcttcacga agacgaattg actcttcatc aatatcattc aatgcattcc
    11161 agtcattaac tttttcttgc atcaagtagt cagcaccgta aacagctaga cgagcatata
    11221 caccaatgat acgtccgcgt gaataagcgt ctggaagacc agtaacagtg tgtgcatggc
    11281 gagcacgacg aatgttagaa gtataagcgc ggaagatacc atcatttact gttgtagcgt
    11341 acttagtgaa gatttcgtga acagctggat caggttcata accattttct ttaagtgtag
    11401 tttccgccat acggatacca cctttaggca tgaaattcaa tttgaaaagc tcgtcgtttt
    11461 gaataccaaa gataagctcg ttatctttgt cgataaaacc agctggtaat tcagaaattg
    11521 aagcaacacg agtatccatt gggaagcgag tttcttcata gtgagctttt gtttcttcaa
    11581 tcactttttt gatgtgaagt gaacgttcag ttgctccagc taagaagctt tcatctccat
    11641 cgtaaggtgc atagtttgct tgaacgaagc gagcaatact tgctttttct ttccaatcaa
    11701 cacctttaaa gccttcccaa gcttgttcaa agatatctgt gtttgtttta actgttgcca
    11761 tagttttctc cttaaacatt gtgacagatt taattttgtt tacatatcca ttttaggaaa
    11821 aagtaaacgt tttcgcaagc atatttcttg acaaaattat gattctttta taatacagtt
    11881 tttttaatct gtatcattaa gttgtaatct gttataatgg agtagagaga atgggggaga
    11941 gaacaatgtt aatttttcct ttaataaatg atacgtcgcg taaaattatc cacattgata
    12001 tggatgcttt ttttgcatct gtggaagagc gtgacaatcc ctctcttaaa ggaaagcctg
    12061 ttattattgg ttctgatccc agaaaaaccg gcggcagagg ggttgtttct acttgtaatt
    12121 atgaggctcg aaagtttgga gttcattcag ctatgtcttc aaaagaagcg tatgaacgat
    12181 gccctcaagc tatctttatt tcgggaaatt accaaaaata tcgccaagta ggaatggaag
    12241 tacgagatat atttaaaaag tatacggatt tggttgaacc gatgagtata gacgaagctt
    12301 atttagatgt aactgaaaat aaaatgggaa taaaatcagc tgttaaactt gctaaaatga
    12361 tacaatacga tatttggaat gatgtccatt tgacctgttc agcgggtatt tcctacaata
    12421 aatttttggc aaaattggct tctgactttg agaaaccaaa aggacttacc ttcatcctac
    12481 cagatcaagc tcaagatttc ttgaagccct taccaataga gaaatttcat ggtgttggta
    12541 aacgctccgt cgaaaaatta catgctttag gggtttatac tggtgaggac ctcctttcct
    12601 tatctgaaat ttctttaatt gatatgtttg gtcgtttcgg gtatgacctt tatcgtaagg
    12661 caagagggat taatgcttcc ccggtaaaac ctaatagagt tcgtaaatca ataggaagtg
    12721 agaaaaccta tgggaaatta ctatataacg aagcagatat caaagctgag atttcaaaga
    12781 acgttcagag agttgttgct tctctggaga aaaataagaa agttggtaag acaatcgttt
    12841 taaaagttag atatgcggat tttgaaacat tgacgaaacg tatgacatta gaagagtata
    12901 ctcaagattt tcaaatcatt gatcaagttg ctaaagcgat atttgatacc ttagaagagt
    12961 cagtttttgg catacgactc ttaggtgtta cagtaacgac cctggaaaat gaacatgaag
    13021 ctatctattt agatttttaa aagttgggac gatgactata tttaactctc tttgaaaaga
    13081 aaaaagcact agattctagt gcttttactt ttagttagtc tcttcaagat cacgttcgtt
    13141 gagtttttgt gctttttgat aagcatggaa ataaagaaga ccgaaaatag cttggaagac
    13201 gatatttata gctattgccc atggtgcttt agtagttaga aaacccaaaa taacaagtcc
    13261 tacagtaatg gcgaagccaa ggtaataagg aaggtaatta accatttgat ttcttttaat
    13321 tgctcttaaa tttttaatac agaaaataac aatggcaatg ttgactaata ctattaaagc
    13381 agtgacaaat agcataaatg gtgttgtggc gctcaagact gcttcgatat gttctttagg
    13441 aagattacct gtgttccctt tattaatttc agttttggct agctgcactg aaattcctgt
    13501 aaacaatctg aatactaaag acaaactatt taatacaatg atagtgattg tagctgtgcg
    13561 cagagagcgg cggacttttt catatgaaac cataa
//
LOCUS       DATRME010000052        13023 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRME010000052 DATRME010000000
VERSION     DATRME010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13023
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..898
                     /locus_tag="VBC23_001478"
     CDS             <1..898
                     /locus_tag="VBC23_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO6843419.1"
                     /translation="DKENTRIFICDTITDYETWVKILNDQGIRTESFKEFAYREQLQN
                     KNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIGRYMVYLGKNYHLIEDGYNCFQAK
                     LFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVNSLVGLPHDIRSKKYKELPRKKLF
                     DSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLAQDKCYKTPTERFQSIQEQYDYFD
                     DIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPSNVPMEIIELMSTGRFECGITHSS
                     TALDFLTCVDKKITLVDLKDIK"
     gene            895..2295
                     /locus_tag="VBC23_001479"
     CDS             895..2295
                     /locus_tag="VBC23_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6843420.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2295..3320
                     /gene="neuB"
                     /locus_tag="VBC23_001480"
     CDS             2295..3320
                     /gene="neuB"
                     /locus_tag="VBC23_001480"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6843421.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3397..4551
                     /gene="neuC"
                     /locus_tag="VBC23_001481"
     CDS             3397..4551
                     /gene="neuC"
                     /locus_tag="VBC23_001481"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6843422.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4548..5177
                     /locus_tag="VBC23_001482"
     CDS             4548..5177
                     /locus_tag="VBC23_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6843423.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5188..6429
                     /gene="neuA"
                     /locus_tag="VBC23_001483"
     CDS             5188..6429
                     /gene="neuA"
                     /locus_tag="VBC23_001483"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6843424.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLFVDEVKTL"
     gene            6543..7028
                     /locus_tag="VBC23_001484"
     CDS             6543..7028
                     /locus_tag="VBC23_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843425.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            7127..7780
                     /locus_tag="VBC23_001485"
     CDS             7127..7780
                     /locus_tag="VBC23_001485"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6843426.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(7846..8484)
                     /gene="plsY"
                     /locus_tag="VBC23_001486"
     CDS             complement(7846..8484)
                     /gene="plsY"
                     /locus_tag="VBC23_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6843427.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            8598..10547
                     /gene="parE"
                     /locus_tag="VBC23_001487"
     CDS             8598..10547
                     /gene="parE"
                     /locus_tag="VBC23_001487"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6843428.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            10681..>13023
                     /gene="parC"
                     /locus_tag="VBC23_001488"
     CDS             10681..>13023
                     /gene="parC"
                     /locus_tag="VBC23_001488"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6843429.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKT"
BASE COUNT         4358 a         1987 c         2502 g         4176 t
ORIGIN      
        1 agataaagaa aatactagaa tatttatttg tgacactatc acggattatg aaacttgggt
       61 aaagatatta aatgatcagg gaataagaac tgaatcattt aaagagtttg cttatcggga
      121 gcagttacaa aataaaaata ttgaagaagt gatggagctt gtagattcag acttaaatca
      181 ctactttgag agagtagata cacaagtcta tctatttaat gatgatactt taataggtcg
      241 ctatatggtg tatttaggta aaaattatca tctaattgaa gatggttata actgttttca
      301 agccaagtta ttcttgggtg gttctgtcgt aaaacgagta attaaaactt acttatttaa
      361 aaagtatgtt ccgtatggtt tttcaaaata ttgtctctca attgaggtta attctttagt
      421 ggggttacct catgacataa ggagtaagaa atataaagaa ctaccgagaa aaaaattatt
      481 tgatagtctt aacaaagaac aaaaatcact gattttcaaa atatttaaaa caaaaccatt
      541 aactataact ccaaagtcag tattattgtt gacacagcca cttgcacaag ataaatgtta
      601 taaaacacct acagagaggt ttcaaagtat tcaagagcaa tacgattatt ttgacgatat
      661 tgtccaggaa tatagaacgt tagggtacaa tgtttattta aaagttcatc ctagagatgt
      721 agtagattat tccaaattgc cggtagagct attaccatca aatgttccta tggaaattat
      781 agagttgatg tcaacaggtc ggttcgaatg tgggataaca cattcgtcca ctgcgctgga
      841 ttttttaact tgtgttgata aaaaaataac tttagtagat ctgaaggata ttaaatgaat
      901 actaagaaat tacttcaatc tggttttatt tatacactcg ggaatttatt ggtgcaagga
      961 ttagccttta tcacacttcc catctatact cgggttatct caacagaagt ttatggccaa
     1021 tatagtttat atgtcgcttg gatgaatatt attatgcttt ttattggcct tcagacgagc
     1081 ggttctttaa gttcagcccg ggtcaaatac ggagaagaat ttaaaagcta ttcagggagt
     1141 gccttttctg taggtaatat atggtttctt attatacttt tgatagcttt tctatttaga
     1201 agttttcttg caccattagt tggtttttct gaatctattt ttttattaat ggtgtgtcaa
     1261 agttacgcta gctatgtggt gactttcttt ggtcagtatt ttatacaaca acagaggagt
     1321 ttggctaatt taatattatc cttagccaat gcagtttcat ctgttgcact atctctattt
     1381 ttaatttttc attggtccga tgacttttta tctagggttt ttggagcttt tgttcctact
     1441 ataataactg gaatagttgc ctttgcttat atttattatc atagcaaatc tttttacaat
     1501 cctaagtatt ttcggttcat tgtcactgtg tctgttccct tgattttcca tttgttagga
     1561 caccagttgt tggggcaatt agatagaatt atgctagctc gtttatacaa tactaaagaa
     1621 gttgcaatgt atagtttcgg ttattcgctt ggaatgatta ttcagattgt tttgaatagt
     1681 attaatatgg cttggattcc atggtttttt gatgctagaa aagaaaaatt actgcaactt
     1741 tcaacttata ttagccgtta tctctactta ggagttttct taacattagg ttatttgact
     1801 gtttttcctg agttagctca aataatggga ggagacaagt atagtagtag tgttcaattt
     1861 atttctttaa ttattgtcag ctacttttta gtttttctct atacatttcc tgtaaatatc
     1921 caattcttct atgctaatac gacatggatt cctattggga cattattagc tgctggtgta
     1981 aattggttac taaaccttgt attaattcct cattatgcag cctatggcgc tgctatggct
     2041 acaataatat cttatttagc cttgttgatt tttcatcata ttgtttcgaa agttaaatac
     2101 cattattcag atgtctcagt taggcagtac atcattttaa gtggtatagt atttagttat
     2161 gctatgttaa tgaacatgtt cctagataat atcgttatta gatggagttt aggaataatt
     2221 attcttatag tatatagcat tgtttttcaa aaagtcattt tagacttatt gagtaaaaaa
     2281 agaaggagaa gataatggtt tatattattg cagagattgg ttgcaatcat aatggagata
     2341 ttaatcttgc gaaaaaaatg gtagatgttg ccgtgtcttg tggtgttgat gctgttaaat
     2401 ttcagacttt taaagctgag aaacttattt ctaaatttgc tcccaaagct gaatatcaaa
     2461 aagcaactac aggaacagca gacagtcaac ttgagatgac gaaacgttta gagttaagct
     2521 ttgaagaata cttagaaatg cgtgattatg caatttcaaa aggtgtggag accttttcaa
     2581 caccttttga tgaagagtca ttagagttct taatttctac agatatgcca atttacaaaa
     2641 ttccatcagg agaaatcact aatttacctt acttagaaaa gattggcaag caacaaaaga
     2701 aagttattct ttcgacgggt atggcggtaa tggaagagat ccatcaagcg gtgaatattt
     2761 tacgtcagaa tggtacaacc gacatttcta ttttacattg tacaacagag tacccaacac
     2821 cttacccctc tctaaattta aacgttattc atactttgaa agatgaattt aaagatttaa
     2881 cgataggtta ttcggatcat tcaattggat cagaagtacc tatcgcagca gcagcaatgg
     2941 gtgcagaagt tattgaaaaa cactttactt tagatactaa tatggaaggt ccggatcata
     3001 aagccagtgc aacacctgat attttagctg ctttagttaa aggggttcgc attgttgaac
     3061 aagccttagg tagatttgaa aaaatcccag atccagtaga agagaaaaat aagattgttg
     3121 ctcgtaaatc agtcgttgct ttaaaaccaa ttaaaaaagg cgatatttat tcaatagaaa
     3181 atattacggt gaagcgccca ggtaatggta tttctcctat gaactggtat gacatcttgg
     3241 gacaagaagc gcaagatgat ttcgaagagg atgaagttat tcgtgattca cgctttgaaa
     3301 atcaattgcc cgagttataa taacacctaa ttataatcgg caacagatgt attacacggc
     3361 taagattatg taattactat atatagaagg gatacgatga aaaaaatttg tttagtaacc
     3421 ggatcgcgtg ctgaatacgg aataatgaag ccattgattc aaagattatc aaaagataaa
     3481 gaagtcaact tacaaattat tgcaacagca atgcatctgg aagaaaagta cggctatact
     3541 tatcgtcaaa ttgaagaaga cggttttgat attgcttata aagttccctt acatctttat
     3601 gatactgaca gaagaactgt atctactgca atggcgcatt tacaactagg attgaccaaa
     3661 atttttgaca aggaagacta tgatctagtc atcattttag gggatcgtta cgaaatgtta
     3721 ccagttgtga atgtagcgtt gatttataat gtcccagtat gccaccttca tggaggggag
     3781 acctcattag gcaattttga tgagtatatt cgccatgcaa ttactaagat gagtcaccta
     3841 cacttagtct ctacagagga ctttcgtcaa cgtgtgattc agatgggaga acaacctcaa
     3901 tttgtaatta acacaggagc tctcggagtg gaaaatgctc tatcaatccc ttctctaacc
     3961 aaagaagcaa tagaaaagca attaggtatt gtactagagg aatcctattt tgttgttctc
     4021 tatcatcctg ttacttttga acaaggaaaa agtgctggag aacagatgaa agcggtacta
     4081 tcagcccttt ctaaatttgg tgtccagtgt ctatttatag gatccaattc agatactggt
     4141 tctgatgata ttgcaaaggc tatcaatact tatttgataa atcatgaaaa tagttattgc
     4201 tttgcttctt tatcaaccca actttatcat tccttaattc gtcattcact aggactgatt
     4261 ggtaattcgt cttctggttt aatagaagtt ccttctttga tgaaacctac tcttaatatt
     4321 ggtgatcgcc aaaaaggtag gctacatgga gaaagtgtag taagtgtacc agtcgaaacc
     4381 ccctctgtat tggagggatt atcaaaattg aatgaagtga ccaactttga caatccctac
     4441 tacaaagaga atgcttcatc tattgcatat gaagcaataa agctttattt gaaagatgaa
     4501 ccttcaattt cccaaccatt ttatgattta aaggagaata atctaaaatg aaaaaaaagt
     4561 tggcattttt aggcgcagga agtcatgctg atgcgattgc tccggttatt gatcctcttg
     4621 tgtatgattt cgtaggtttt tttgatgata aagatattac ggagcatgat ggttatcctg
     4681 ttcttggaaa actttatgat gtgctacctt accttgaaga tggctcaata gatgcagtat
     4741 ttattacaat aggtgataat gctaaaagga aagaactatt tgaatatgta gcaaaggatt
     4801 attatgactt tattattaac atcattagtc ccaatgcttt agtattgaca ccagatagta
     4861 tttgtgggcg tggtatcttt attggttttg gggcttttat aggttctaaa gtgaagctgt
     4921 ttgataacaa cgttgttaat acaggagcgc tcattgaaca tcatactgtt gtagaatcac
     4981 actgtaatat agcacctaat gctaccataa atggtctttg ttatattaga gaagaagttt
     5041 atgtaggtag tgccagtgtt attattcaaa ccttggatat ttcatcgtgt acgacaattg
     5101 gcgcaggagc tgttgttgta aaagatatta tagaacctgg aacctatgta ggggttccag
     5161 ccaaaaaaat aaaataaagg atatgttatg aagccaattt gtattattcc tgcgcgatca
     5221 gggtcaaaag gattaccaga taagaatatg ttattcctag caggtaaacc catgattttc
     5281 catactatag atgctgcaat tgaatctgga atgtttgata agaaagatat ttttgttagt
     5341 acagattcag aattgtacag agagatttgt ttagaacgcg gtatttcagt ggtgatgaga
     5401 aaaccggaac tttcaactga tcaggcaact tcgtatgata tgttaaaaga ttttttatct
     5461 gactatgaag ataatcagga gtttgtgtta cttcaagtaa cctctccact aagaaaatca
     5521 tggcatataa aggaagcaat ggagtattat tcttcacatg atgttgacaa tgttgtaagt
     5581 ttttctgaag ttgagaaaca ccctggtctg tttacgacat tgtctgataa aggctatgct
     5641 atagatatgg tgggagcaga taaaggttat cgtcgccaag atttacaacc tttatactat
     5701 ccgaacggcg ctatttttat ttctaataaa gaaacttact taagggaaaa aagctttttc
     5761 acctctagga catatgctta tcaaatggca aaggaatttt cattagatgt tgatacgaga
     5821 gatgatttta tccacgtcat cggtcacctc tttttcgatt atgccattag ggaaaaagag
     5881 aataaagttt tttataaaga aggctatagt cgtttgttca atagagaagc ttcaaagata
     5941 attttaggtg attcaaaaac gatatctatc tcactagaaa attaccataa ttattcccaa
     6001 ggtggtgtaa cattagcaac gatgttggaa aacttaccta actttttgac agctaatgta
     6061 acagaggctt ttgtttctat aggggtaaat gatctcatta caggtcatag tgtggaagaa
     6121 atattcagca atttccaaaa actctactca ttattagctg agaataaaat aaagatgaga
     6181 ttgacaacta ttgcttacac cctttttaga gaaactgtca ataatgcaga tattgagaaa
     6241 atcaatcaat ggctaacaga attttgttat caaaatcaga ttccactgtt agatattaat
     6301 agatttttat caaaggatgg taatctaaat tatcatttaa ctagtgatgg attacatttt
     6361 actcaagagg ctaatgattt gttacaaagt caatatcaat tatttgtaga cgaagttaaa
     6421 accttataat atttatttta taaaattatt catcttattt ttgcgatttc aatagtgata
     6481 tggtatattt aaagtaagct acgtatagtt ttaccagtca gaaatagtaa ggatggttga
     6541 gtatgagaga aactgaaaca cacgatcacc aagcgctaat tcaaaaattg ttagtcagta
     6601 ttcattattt aaccttgttt cgtgatgaaa ttatattagt agaaaaaacc ccatctttac
     6661 tggggaaaca tttttctatt gctattgtgc agaatgaact aggtgagata ctttctaaaa
     6721 tagaagcttt gagtaagcaa aaaaaactga ttcgttctat ttattggtat gatgaatcgt
     6781 catttaaagt catgaataag gctctggcta ttgtagaaga atggattaag ggcttagata
     6841 atcttttaga attctgtcaa tcacagacgg tttttcaagc aattttaggt gatgaacgtg
     6901 cacatgtatt tggaatcctt attgatgttt atacttcttt aaacattata aatacttctt
     6961 tgaaagaaga aaattctaga ccagtgtcct tatctgattt ggctctaggt cttaaggacg
     7021 aagattaata aaaaaacgtt actgctttga atgaataaag cagtaacgtt ttttataaaa
     7081 tattgctata ctagtaatac agatatctaa aagtaaggag ttataaatga aacattccag
     7141 ttggcatgat ttaataaaaa gagaactacc caatcattac tacaataaaa ttaatacatt
     7201 tatggatgca gtttacgaaa gcggaatcgt ttatcctcca agagataagg tgttcaatgc
     7261 catacagata acaccactag aaaacgtaaa agtagtgatt attggccaag atccttacca
     7321 tggccctcaa caagctcaag gactctcatt ttctgttcca gacaatttac cagctcctcc
     7381 ttcacttcaa aatattctaa aagaattagc agaagatatt gggtcacgtt cacatcatga
     7441 cttaacatca tgggcgcaac aaggagtgct gctattgaat gcttgtttaa cagttccaga
     7501 acaccaagct aatggtcatg ctgggcttat atgggaacct tttactgatg ctgtcatcaa
     7561 agtggttaac caaaaggaaa ccccagtagt ctttattctg tggggtggct atgctcgaaa
     7621 gaaaaagtcg ctaattgata atcctattca tcatattatt gagagtcctc atccgagtcc
     7681 cctatctgct tatcgtggtt tctttggtag cagacctttt tctagaacaa atcatttctt
     7741 agaagaagaa ggaataaatg agattgattg gttaaattag aagggaaatc tactctcgaa
     7801 acattgttag tcttataata aaaaaccgct agtagcggtt ttttattatt tatttttttg
     7861 tttacttagg tttaagccaa acggaaccaa attttcttgc ctttttcgaa tacgtttgat
     7921 attatcctga tgtcggataa taatcgtgag ggccattaag ataaccacag tggtaaatat
     7981 ccagtcatag tccgttaata taaaacctac taatggaaaa attaagacac taaggatacc
     8041 tacgacagct actgtaatac tggatagcga aatcatgcta aaaagataaa gcgttaataa
     8101 aaagatcact aataagtaaa ggaaaaaaga aggtgcaaat cctagcaaaa ccccagcgct
     8161 tgtagcaacg gctttcccac ctttgaattg tgcaaaaata gggaaagtgt gacctataat
     8221 agcaaaaaaa ccgataaaaa atggagatac tgtcgttaca cctaatatga taggaataag
     8281 tgttgctaga gtccctttta aaatgtcaat agtcaaagtg acaatgccag cttttacacc
     8341 taaaatacgg aaggtgttcg tggtgccagt attaccacta ccgtgttgac gaagattgac
     8401 ttggtaaaaa tatttaccaa tccaaagccc agtttggata gagcctaaca gataggcaat
     8461 gataatcata attataatat tcataccctt attataacat aagcataaaa gtctttttgc
     8521 tatttttaaa aaaataattt tgcaaaaaca gcaaaaaact tgtaatataa aaaagatgaa
     8581 aaaatttgga ggtcactttg actaaacaag atataacagt gacgaattat ggcgacgatg
     8641 ctattcaagt cttagaagga ttagatgctg ttcgtaagcg tccagggatg tatatcggtt
     8701 ccacagatgg aactggttta catcatttag tgtgggaaat tgtagataat gcagttgatg
     8761 aagctctatc aggttttgga aatcgcattg atgtgattat taacaaagat gggagtataa
     8821 ctgttacaga ccatggacgt ggtatgccaa ctggaatgca tgctatggga aaacctactg
     8881 ttgaagtcat cttcacggtg cttcatgcag gtggaaaatt tggacaaggt ggttataaaa
     8941 catcaggtgg tctccatggt gttggatcat ctgtagttaa tgctttgtct tcttggttag
     9001 aagttgaaat cattcgcgac ggagctattt acagacaacg ttttgaaaat ggtggtaagc
     9061 ccgttacaac tctgaaaaaa attggcacgg caccaaaatc aaaatctgga acttctgtca
     9121 gctttatgcc agatcaatca gtattttcaa cgatagattt taagtttaat acaattgcag
     9181 agcgcttaaa ggaatctgcc tttctcttaa aaaatgttac tttaacctta acagataatc
     9241 gttcagaaga agcagagcat ctagaatttc attatgaaaa cggtgttcaa gattttgtag
     9301 agtatttaaa tgaagataaa gaaacgttga caccaatcat gtttttcgaa ggtgaagaac
     9361 aagaatttca tatcgaagtt gctttgcagt ataatgatgg tttttcagat aatatccttt
     9421 cttttgtcaa taatgttcgt actaaagatg gtggaacaca tgaaactggc ttgaaatcag
     9481 ctattacaaa gtctatgaat gactatgcac gtaaaacagg acttcttaaa gaaaaagata
     9541 aaaaccttga aggttctgat tatcgtgagg ggctatcagc cattttatct attcttgtac
     9601 ctgaggagca cttgcaattt gaaggtcaaa ctaaagataa actaggaagt cccttagcac
     9661 gaccaattgt tgatggtatt gtttctgaaa agttaactta ctttcttatg gaaaatggag
     9721 atttagcatc taatttaatc cgtaaggcaa taaaagcacg tgatgcgcgt gaagctgcac
     9781 gtaaagcacg tgatgaaagt aggaatggga aaaagtcgaa aaaagataaa ggtctacttt
     9841 ctggaaaatt aacccctgca caatcaaaaa atgctaagaa aaatgaactc tatttagtcg
     9901 aaggagactc tgcaggtggc tctgctaagc aaggtcgtga tagaaagttt caagctattt
     9961 taccgttgcg aggaaaagta ttgaatacag ctaaggcaaa gatggctgat attataaaaa
    10021 acgaagaaat taatacaatg atccatacca ttggtgcagg tgttggtcct gattttaatc
    10081 ttgacgatat taattatgat aaagttatca ttatgaccga tgctgatact gacggagctc
    10141 atattcagac actattatta actttctttt atcgctatat gagacctctt gttgaagagg
    10201 gacatgtcta tattgctttg ccacctttgt ataaaatgtc aaaaggaaaa ggtaagaaag
    10261 aaattgttga gtatgcttgg acggatatag agttagaaga attacgacaa aaatttggta
    10321 aaggctcact attacaacga tataaagggc ttggagaaat gaatgccgat caattgtggg
    10381 aaaccactat gaaccctgag acaaggacat taattcgtgt aacaattgaa gatttagcaa
    10441 gagcagaacg tcgagttaat gtcttgatgg gtgataaagt tcctccaaga cgccaatgga
    10501 ttgaagataa tgtcaagttt actttagaag agaatacagt attttaataa agagccttgg
    10561 ttattcatgt tttatagtgt acaagtaata ttgtatgcgt tagacaagag agccaatcat
    10621 tcatttcaaa taagtggaaa aataatacta agccactcca actaggagaa aattaaaaat
    10681 atgtctaata ttcaaaatat gtcccttgag gacattatgg gagagcgttt tgggcgctat
    10741 tctaagtata tcattcaaga acgggcgttg ccggatattc gtgatggctt aaaacctgtt
    10801 caacgtcgaa tcctttattc aatgaataaa gatggtaata cttttgaaaa gggatttcgc
    10861 aaatctgcaa aatcagtcgg taatgttatg gggaatttcc atcctcatgg ggattcctct
    10921 atctatgacg cgatggttcg tatgtctcaa gattggaaaa atcgtgagac attgattgaa
    10981 atgcacggaa ataatggttc tatggatgga gatcctgcgg cagctatgcg ttatacggaa
    11041 gcccgtctat cagaaattgc tgattatctt cttcaagata ttgataagaa tactgtaccc
    11101 tttgcgtgga attttgatga tacagagaaa gaaccaactg ttttaccagc tgcctttcct
    11161 aatcttttag tcaacggagc aacaggaata tctgcaggct atgcgacaga tattccacca
    11221 cataatttag cagaagtcat tgatgctgtc gtgtacatga ttgatcaccc taaagctaaa
    11281 ttagataaat taatggaatt tctacctggt ccagattttc caactggcgc tatcattcaa
    11341 ggaaaagatg aaattcgtaa ggcatatgag actggtaagg ggagagtagc ggttcgctcg
    11401 cgaactgcta ttgaaacctt aaaaggtggt aagaaacaaa ttattgttac tgaaattcct
    11461 tatgaagtta ataagtcagt tttagtaaaa cgaatcgatg atgttcgtgt taataataaa
    11521 gttccaggaa tagcagaagt gcgtgatgag tctgatagag atggccttcg tatcgctatt
    11581 gagctcaaaa aagaagccga tgaaacaatt gtcttaaatt atctttttaa atatacagac
    11641 ctacaagtta attataattt caatatggtt gctattgatg actatacccc taagcaggtg
    11701 ggtctatcaa gaatattaac tagctatatc gctcaccgaa gagaaattat tattgctcgt
    11761 tcaaaatttg ataaagaaaa agccgaaaaa cgtctccata ttgttgaggg actgattagg
    11821 gttctttcta ttttggatga agtgattgca ttaattcgtg cttctgagaa taaagctgat
    11881 gcgaaagaga atttgaaggt cagctatgag ttttcagaag ctcaagcaga ggctatcgtc
    11941 acattgcaat tatatcgtct cacaaatact gatattgtga cgttgagaga agaagaagaa
    12001 gaacttcgtc aacaaatcac aatgctcaag gcaattatta gtgatgaacg tacgatgtat
    12061 aacgtaatga aacgtgagtt acgtgaggtc aaaaagaaat ttgccaatac tagacgttct
    12121 gaacttcaag aattagctga aactattgaa atcgatactg caagtttaat tatcgaagag
    12181 gacacatacg tcagtgttac tagaggcgga tatgtaaaac gcaccagccc tcgctctttc
    12241 aatgcatcaa ccgttgatga acttggtaag cgagaagatg acgaattgat atttgtgagc
    12301 aatgcaaaga ctacccaaca tttattgatg tttactaacc ttggtaatct tgcttatcgt
    12361 ccagttcatg aattagctga tattcgatgg aaagacgttg gtgaacattt atcacaaaat
    12421 cttgttaact ttgcttcaaa tgaagaaatt atttatgctg aattagtaga tgattttact
    12481 aaagaaactt attttgctgt cactagttta gggcaaataa agcgttttga acgtcaagaa
    12541 atcagtccat ggcggacata taagtcaaaa acagctaaat atgctaagtt aaaaaatgta
    12601 gatgattacg ttgtgactgt tgctcctatc caattagagg atgttatact tgtcacttat
    12661 aatggttatg tgttgagatt tagtataaat gatgtaccag tcgttggtag taaagccgca
    12721 ggtgtaaaag cgatgaattt aaaagatagt gatcacatcg tatctgcatt tatagctaac
    12781 accacaagtt tatatttatt aactcataga ggaagtctta aacgaatggc cattgatgtt
    12841 atccccacaa ctagtcgtgc taatcgagga cttcaagttc ttcgtgaatt gaaatccaaa
    12901 ccgcatcgcg tttttaaagc aggacctgta tatttagagg attcatcttt tgaatttgat
    12961 ttatttagtt cagtatcgaa tcatgaagga gatactttcg tacttgagat tatgtctaaa
    13021 aca
//
LOCUS       DATRME010000053        12925 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRME010000053 DATRME010000000
VERSION     DATRME010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            149..1132
                     /gene="guaC"
                     /locus_tag="VBC23_001489"
     CDS             149..1132
                     /gene="guaC"
                     /locus_tag="VBC23_001489"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6843430.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     regulatory      1188..1283
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1387..1968
                     /locus_tag="VBC23_001490"
     CDS             1387..1968
                     /locus_tag="VBC23_001490"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6843431.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     gene            1968..3242
                     /locus_tag="VBC23_001491"
     CDS             1968..3242
                     /locus_tag="VBC23_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6843432.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(3341..4129)
                     /locus_tag="VBC23_001492"
     CDS             complement(3341..4129)
                     /locus_tag="VBC23_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6843433.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(4228..5463)
                     /locus_tag="VBC23_001493"
     CDS             complement(4228..5463)
                     /locus_tag="VBC23_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6843434.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            complement(5487..6089)
                     /locus_tag="VBC23_001494"
     CDS             complement(5487..6089)
                     /locus_tag="VBC23_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6843435.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            complement(6107..7045)
                     /locus_tag="VBC23_001495"
     CDS             complement(6107..7045)
                     /locus_tag="VBC23_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6843436.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            complement(7194..7376)
                     /locus_tag="VBC23_001496"
     CDS             complement(7194..7376)
                     /locus_tag="VBC23_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6843437.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            7514..8083
                     /locus_tag="VBC23_001497"
     CDS             7514..8083
                     /locus_tag="VBC23_001497"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6843438.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            8118..9197
                     /gene="prfA"
                     /locus_tag="VBC23_001498"
     CDS             8118..9197
                     /gene="prfA"
                     /locus_tag="VBC23_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6843439.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            9197..10027
                     /gene="prmC"
                     /locus_tag="VBC23_001499"
     CDS             9197..10027
                     /gene="prmC"
                     /locus_tag="VBC23_001499"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6843440.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            10020..10616
                     /locus_tag="VBC23_001500"
     CDS             10020..10616
                     /locus_tag="VBC23_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6843441.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            10708..11964
                     /locus_tag="VBC23_001501"
     CDS             10708..11964
                     /locus_tag="VBC23_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6843442.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            11969..>12925
                     /locus_tag="VBC23_001502"
     CDS             11969..>12925
                     /locus_tag="VBC23_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6843443.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNI"
BASE COUNT         4214 a         2041 c         2480 g         4190 t
ORIGIN      
        1 aaaacgaaca ttattcaaat aaattgatca aaaattgact aaaaataaat aaagtatata
       61 atttaaaaag gaaaggtatg tttttaccta gcgatgcttt gttatacaaa gttataggaa
      121 ggtcatttta ctcattagga gaaccattat gtttaacgat attcctgttt ttgattatga
      181 agatattcaa ttaataccaa acaagtgtat tatatctagc cgctcacaag ctgacacaag
      241 tgtcaaactt ggaaactata cctttaaact tccagtaatc cctgctaata tgcagactat
      301 tattgatgaa gaggtagctg aaacattagc gtgtgaaggt tatttttata ttatgcaccg
      361 ttttaatgaa gaagcaagaa gaccttttat caagcgtatg catgataaag gcttaattgc
      421 ttcgatttcg gttggggtca aagactatga atatgatttt gtgacaagtc tgaaagaaga
      481 tgctcctgag tttatcacta ttgatattgc acatggccat tctaacagtg ttatagagat
      541 gatccaacac attaaacaag agcttccagg aactttcgtt attgctggaa atgtaggaac
      601 gcctgaagct gttagagaat tggaaaatgc aggtgcagat gcaactaaag ttggtattgg
      661 acctggtaaa gtttgtatta ctaaagtcaa gacaggtttt ggtactggag gttggcaatt
      721 agctgcctta cgttggtgtt ctaaagctgc tcgcaagcca attattgctg atggtggtat
      781 tcgaacacat ggggatattg caaaatctat ccgctttggt gctagtatgg taatgattgg
      841 ttcgctgttt gctggtcacc ttgaaagtcc tggaaaactt gtggaagtag aaggacagca
      901 atttaaagaa tactatggct cagcttctga atatcaaaag ggagaacata aaaatgttga
      961 aggtaagaaa attctattac ctgttaaagg gcgactagag gatactttga cagaaatgca
     1021 acaagatttg caaagttcta tttcttacgc aggtggaaaa gaattggata gcttgagaca
     1081 cgttgattac gttattgtta aaaattctat ttggaatggt gattccattt aaattaaatc
     1141 ttgaaatctt aattaaaaat gttacaatag ttatctatca tacataacaa ttaaatatat
     1201 gatttactta tttatgctga ggattggctt agcgtctcta caagacaccg taatgtctaa
     1261 caataagtaa gctaataaat agcttgcctg ttggttcatg ttaatgaagc gataaattag
     1321 aggagatttg tgtcctctga ttgaggtaag ttattttttt attttctaaa gagtgaaggg
     1381 gttattatga agttattgga agaacgtatt cttaaagatg gtgatgtttt aggtgagaac
     1441 attttaaaag ttgattcttt tttgactcat caggtaaatt ttgagttaat gcaggaaata
     1501 ggtaaagttt ttgctgataa atataaagaa gccggcatta cgaaggttgt tacaattgaa
     1561 gcatctggaa ttgcgccagc agtgtacgca gctcaagcat tgggcgtacc aatgatattt
     1621 gctaaaaaag ctaagaacat tactatgact gaaggtatct taactgctga agtgtattct
     1681 tttacaaagc aagttacgag tcaagtttct attgtgagtc gctttttatc taacgatgat
     1741 actgtactca tcattgatga ctttttagca aacggtcaag cggctaaagg attacttgaa
     1801 attattggtc aagctggagc taaggttgct ggtatcggaa tcgttattga aaaatctttc
     1861 caagatgggc gtgatttgtt agaaaaaaca ggtgttccgg ttacttctct tgctcgtatt
     1921 aaagcgtttg aaaatggtcg agttgttttt gcggaggctg atgcttaatg aaatttaatg
     1981 agcaaagtaa ttcgcaagca gcacttctag gcttgcaaca cctgttagct atgtatgcag
     2041 gatccattct tgtacctatc atgattgcta gtgctcttgg ttataatgct aagcaactaa
     2101 cttatcttat tgcgacagat atttttatgt gtgggattgc caccttatta caattacgat
     2161 taagtaagca ctttggagtg ggtcttccag ttgtattagg gtgtgccttt cagtctgtag
     2221 ctcctttatc aattattggt gcacaacaag gttctggcta tatgtttgga gctttaattg
     2281 cttcaggaat atatgtcgtt ttagttgctg gcattttttc taaagttgca aatttctttc
     2341 ctccaattgt aacaggatca gttattacta caattggttt gacattaata ccagttgcga
     2401 tgggaaatat gggtgataat gcaaaagaac caagcttaca atctttaact ttatcactgg
     2461 taacgattgg tgttgtttta ttaattaata ttttcgcaaa aggctttttg aagtcaattt
     2521 caatccttat tggactcatt tcaggtacaa ttcttgcagc atttatgggc ttagttgatg
     2581 cttctgtggt agcagaagca ccacttgtac atattccgaa gccattttat tttggagctc
     2641 ctagatttga atttacttct attttaatga tgtgtattat tgcaacagtt tctatggtag
     2701 aatcaacagg tgtttacctt gcgctttcag atattacaaa cgataaatta gacagtaaga
     2761 gacttcgtaa tggttaccgt tcagaaggat tggcagtatt acttagcggc ttatttaata
     2821 cctttccata tactggtttt tctcaaaatg tgggactggt acagatatct gggatacgta
     2881 cgcgtaagcc gatatatttt acagctctct tcttagttat acttggcttg ttacctaaat
     2941 ttggcgcaat ggctcaaatg attccaagtc cagttcttgg tggtgctatg ttagttttat
     3001 ttggtatggt agcacttcaa gggatgaaaa tgcttaatca ggttgatttt gagcataatg
     3061 agcataactt tatcattgca gccgtatcaa ttgcagcagg agtaggtttt aatgagacaa
     3121 atctcttcat tagtcttcct aatactttac aaatgttttt gacaaatggt attgttatct
     3181 caaccctgac agctgttgtt ttaaatatca ttttgaatgg attgcctaaa aaattaatat
     3241 gatactttcc tttatttatc aaaaaagtta atttttagtt attgatttca tgatgagaat
     3301 ataaaagaaa aactgagtaa taaattactc agtttttctt ttattcatgg tgagaagttt
     3361 ttgttttatt taaccaagca taggctaggc cgatgaaaac gccccctcct atccagttac
     3421 caaaaaatac aagtgtccat tggcggataa cgtttagaag agtaaaacct ttaatatgtt
     3481 cgatatggct aaaagcagct aacataaagg atgcaaaatt agcaattaag tgttcgtttg
     3541 atagaaaaac aaacataaaa atagcagata aaataaccgt catttttgcc gattcttctt
     3601 ttaataacat gtaagcaagg atagctaaat tcacaaacat attagcaata ataccttcta
     3661 ggaaagctcc tgaactaggt tttcctaact ttttagcaac gacatgccct aaaaagctat
     3721 catttgttaa atgctggaat gagtacgatt gattaaacaa ccaagctaat atacaagcac
     3781 caacgaggtt gaaaaaagta caataaatta aaattgttat ggcttttttc caagagatat
     3841 ttttattata ggctcctgca gtgagataaa gcatattaga tgtcgccaat tcaccattaa
     3901 atattaaaac ataaataagt ccaaaactaa agatgaaagc aaatacaaaa cctgatagag
     3961 caggagaaat tttaccaata gtatcagctg cgacgatacc ggcagcagta ctcattgtca
     4021 aataagcccc tgctagcatt gatctcaagg cgtagcgtcc taaactctct ttataaagcg
     4081 cttccttttt agcgcaattg taagcaattt tttcttgaaa aagtgacata gtatattcct
     4141 tttatgtgat tttattaact tgattaaata ttaaaaagga ggatcttgat atctttttaa
     4201 ggtatcagat cctcaaaagc gaggagatta atgttcagag gcaccagaag tggcatctgt
     4261 ggcttgatca acgggagcat cctctgtgct agttgaagcg ctagaagtag cgtcaactgc
     4321 tgaagctcca ggagcagcag tagttccacc gaccaaacgt tgtccagtag tttggaggta
     4381 ccaatctaac cacggtttaa agttaaagat aatttcatta ataccagcat aagatccgtc
     4441 tgggtagtac atagcttgat agttgtgagt attgataact tctggaggtg taccaggaac
     4501 gactgtacgt acatattctt gatttccatt acgaagagta ccgataaccc attcaacatt
     4561 tttcattcgt gccggtggta aactaccatg aactgtggcc atacggtttc ctacttgttc
     4621 acgaacacgt tttcctaaca ttgtatctgg atctggatga ttattattgt aatatagaaa
     4681 ttgattatta tcatctgcat atgttagctc aaatggcata gcatttaaaa acatattaag
     4741 ttggttaaca gtaagtaaac cattgtctag tttaacgtaa gcatcccctt caacggcgtt
     4801 aactttctcg gctgccttgt ctaaccaatc aggatcatct ggatcgatac tagtaatggt
     4861 tgtatcgatt tgtccctgac agtagaggtc ttcagattca atagctttag gttttctcaa
     4921 tgatgataca actcctacat atttgataaa attatcaaga caagtttcta aaaattgaac
     4981 agtcccagga ttgataatgt ttccattatc atcaaaggct tctttagctt ttcctaataa
     5041 gaattcattt cctggaagtg tgtaagcatt aacgccagga gcatcaagga ttttacgaag
     5101 gtgtatttga gcacgtgatg tgccttgatc ataatatgaa gcaccgacaa tcataacagg
     5161 tttattttcg agtggatgca cagcaaatga gagccactca agcacacttt taagagcggc
     5221 cgtaatagta tgattatgtt caggagtggc aataataacg ccatcagctc gtgtgatctt
     5281 attattaaga agacgaagtt ggaaactttc ttcccaattt tggacttgat taaacattgg
     5341 aatatcatcg atttcaagga cttccaattc aaatttggtt ttaaaattct tgcgaataaa
     5401 ttcaagcaac atacgattat atgattgctc tgcatttgag ccaacaatac ctacaaattt
     5461 catcttagtt attcccttct atactcttat aaactttccc agtcaaaatt ttctgcttct
     5521 ttttgaagta aatcacgagc atttgataat ttaccagtaa tcttaacaaa aagacgaaaa
     5581 tcatcaaaga tagcatctag cttctgactt gtttcgatat cgtgtaggtt gccatcttta
     5641 tcaaaggctt ggagagagtg tgataacaag aattcgtcag gtaaaacact tgcttttagt
     5701 tcaggagcgt taagtatttg acgtaattgt aattgtgcac gtgatgatcc aagtgttcca
     5761 tatgaagcac cagtaatcat aacgggtttg ttgagaagtg ggtaaatacc atatgatagc
     5821 caagctaaaa cactcattaa ggccgaaggt atagaatgat catattctgg cgtaccaata
     5881 ataacaccat cagcttcttc aattttagca gcaatatcta aaattacttg gggaacattt
     5941 ttatcagcag gtttattaaa taacggaagg tctttaactt cgatgagttc aatttctgct
     6001 ttgtcagcaa agtgttgttg catgtattgt aataattgac gatttgttga tttatttgag
     6061 ttagttccaa cgatgccaat aagtttcata ttagaaatcc tctttcttaa tgataaaata
     6121 gttgaaaatg atgttttaag ccatcagata aatagatacg gtcatcttta gtgatgataa
     6181 tgccttcaat gtaggaaaca gcgtttaaaa tatcaagagc ctctttaatg gataagccaa
     6241 acaaccttgt ggtccagatt tcacagtcga cggattgttt agaaacaata gaaatgctag
     6301 ccatctctgt ttgtatcgga taaccagtct gtcgatcaaa aatatgatgg tattccttat
     6361 cgtcaataat cagacggcgt tcataagtac ctgaggttac aacagattgg tttttaattt
     6421 tgacagctcc aatattttgt ccccgtttct ttttcggatg ttgaatgccg ataacccaat
     6481 acccttctga acgattagga ttatctccgt gaactaaaac gtttccgcct aaattaatga
     6541 tagcagaatc aatcatctca tttttgagat aggtcataat tttgtcagca atatatcctt
     6601 ttgcaagtgc acccaaatca attttcattc ctatctgatt taaaaaaaca gattgatttt
     6661 ttgaatctag aagtatatga gttgggtcag ataaaatcat agcttctgat acttcactag
     6721 ggctaggtag tttagcatct gaaaatccaa ttctccaagt ttgcactaaa ggaccaatag
     6781 caatatttag gttactaggc ctagctaagc tgtgttcttt cccaattgtg attaattcaa
     6841 agaggtctgg atgtacttga ataggcttga taccagcgtt gttatttata gccattaact
     6901 ctgaattaaa gtcatttgca ctaaagcgat ttttgtagag ctccaagagt tctatgacct
     6961 cttggatttg tttttgagca tttttagaat taatttgaat atcgatagtc gttcccatga
     7021 gtcttaaact atgagaaagt tgcaaatttt ccacccccaa agcaatatat tctaatgata
     7081 ccgttttcta acttgaaaat caatacttat tttcaaatat tttcaatttg tcaagaaata
     7141 tgcttgcaaa gactatcaaa agaggttacc tgggtaacct cttttactat ttcttatttt
     7201 ttcttaagtt caccttgagg atagtaggtt ccttcaggca tatcgttgat aaaaacatgg
     7261 atattttctt taggtgcttt tgcaatgcgt gatactactt cagtaacctc gcgagctaat
     7321 tcattttttt gttcttgtga gcgtccctca aacaagtcaa tttttacaaa tggcataata
     7381 ttactccttt atgttattgg gcatcattgt aacataatgt tgtgaaaaag tacacactgt
     7441 tttcaatggc tgggaaaact tcagcctttg tggtaaaata ggtagaatac tatagaaagt
     7501 aggattttcc gctttggctc aactctatta taaatatggg acaatgaatt ctggtaaaac
     7561 cattgaaatt ttaaaagttg ctcataacta tgaagaacaa ggaaaacctg tggttattat
     7621 gacaagcgcc ttggacactc gagatgaatt tggagtcgtt tcaagtcgta ttggaatgcg
     7681 tcgtgaagca gttccaattt cagatgatat ggacattttc tcctatattc agaatttacc
     7741 acaaaagcct tattgtgttt taattgatga atgtcaattt ttgagtaaga aaaacgtcta
     7801 cgatttagct agagtagtag atgacttaga tgtaccagtc atggcttttg gtttgaaaaa
     7861 tgatttccaa aacaatcttt ttgaaggttc aaaacatttg ctattattgg ccgataagat
     7921 tgatgaaatc aaaacaattt gccaatactg tagtaagaaa gcaaccatgg ttttaaggac
     7981 agaaaacgga aaacctgtct atgaaggtga tcagatacaa attggtggta atgaaacata
     8041 tatcccagtt tgtcgaaaac attattttaa tccagatatt taaaagataa tcggttttaa
     8101 aggagataca aagaaaaatg aatatttatg atcaattgca ggcagttgaa gatcgttatg
     8161 aagagttagg tgagttactt agtgatccag atgttgtttc agatacaaag cgatttatgg
     8221 aattatcacg tgaagaagct aatactcgag aaactgtcac agcttataga gaatataagc
     8281 aagtcattca aaatatttca gatgctgaag aaatgatcaa ggatgcttct ggtgatgctg
     8341 aattggaaga aatggctaaa gaagaactta aagaatcaaa agcagctaaa gaagagtatg
     8401 aagaaagatt aaaaatcctt ctattaccta aagatcctaa cgatgataaa aacattattt
     8461 tagaaattcg tggtgctgct ggtggcgatg aagcagcgct ttttgctggt gatcttttaa
     8521 caatgtatca aaaatatgct gaaactcaag gatggcgttt tgaagttatg gaatcatccg
     8581 ttaatggtgt tggtggtatt aaagaagtgg ttgctatggt ttctggtcag tcagtttatt
     8641 ctaagcttaa atatgaatca ggagctcatc gtgttcaacg tgttccggtg acagagagtc
     8701 agggtcgtgt tcatacttca actgcaactg ttttggtaat gccagaagtt gaagaagtag
     8761 aatacgaaat tgatcaaaaa gatcttcgcg tcgatattta ccacgcgtct ggtgctggtg
     8821 gtcagaacgt caataaagtt gctactgcag ttcgtatggt acacattcct acaggaatca
     8881 aagttgaaat gcaagaagag cgtactcagc aaaaaaaccg tgataaagcg atgaaaatca
     8941 ttcgtgctcg tgttgctgac cattttgcgc aaattgctca agatgaacaa gatgctgaac
     9001 gtaaatcaac agttggtact ggtgatcgtt cagaacgtat tcgcacttat aatttcccac
     9061 aaaatcgtgt aacagatcat cgtattggtt tgacacttca aaaattagat actattttat
     9121 ctggaaaaat ggatgaagtt attgatgcac ttgttatgta tgatcagact caaaagttag
     9181 aagccttaaa taaataatga attatgcgca gttaatcaag cattatgaac agttgttaga
     9241 ggcttgtggt gaagaagtcg aaaattttat ttatgtatta aaagacctaa aacaatggtc
     9301 aacaactgat taccttttaa atcaaaatag tagtgtgagt gatactgatc agcaattaat
     9361 ggaaaatatt tttcaacagc ttaaaaaaca tagatcaccg cagtatatta caggtaaagc
     9421 ttactttaga gatcttattt tctttgttga tgaacgagtt ttaattccga ggcctgagac
     9481 agaggagttg gttgatctaa tcttatcaga gaataaagta gaagattgtt ctgttttaga
     9541 tattggtaca ggtagtggag caattgccat ttctctaaaa aaagaacgcc cctcttggga
     9601 tgttctagct agtgacattt ctgtatctgc attagacttg gcaaaggaaa atgctaataa
     9661 ttgtgatgcg gaagtgactt ttatagaatc agatgtcttt agtaacattt cagataaatt
     9721 tgatattatt gtgtctaatc ctccctatat ttcttacaat gacaaagacg aggtggggaa
     9781 gaatgtgcta gcttcagagc ctcatagtgc cctttttgca gacgaagaag gattagctat
     9841 ttatcgtaaa atcattgaaa actcaagaga atatttgcaa cctagaggta aattatattt
     9901 tgaaataggt tacaaacaag gtgatgattt acgaagttta ttgaaaagat attttccaaa
     9961 caatcgttgc agagttttga aagatatatt tggtaaagat agaatggtgg tattagataa
    10021 tgaatgattt gggacagatt ttagaggatc atggcgcagt tattatgcca acagaaacgg
    10081 tctacggaat ctttgccaaa gctttgagtg aagaagctgt gaaccacgtc tatgagctta
    10141 aaaaaagacc tcgtgataag gctatgaact taaatatatg tgattttgag actattttaa
    10201 aatattctaa aaatcaaccg acctatttga agcaattgta cgatgctttt ttaccaggac
    10261 cgctaaccat tattttggaa gcttcacaag aagttccgga ttggatcaat tctgggttat
    10321 caagcgtagg atttcggatg cctaagcacc ctgtaaccct tgatatgatt gctaaccatg
    10381 gtccattgat tggtccttct gctaatattt ctggatgtga tagtggtcgt gtattctcag
    10441 aaattcaaaa acagtttaat catcaagttt taggtattga agatgataaa gcattgacag
    10501 gtgtggattc tactattatt gatttgtctg gtgatagagt taagatttta aggcaaggtg
    10561 caatcacgca agaggtacta acggctacga tacctgaatt aatttttgaa aattaattta
    10621 atcatcttgc aactaatagt aaaaatgtga taaatataat gattgaaacc attatttaaa
    10681 agttataacg agcttaagga gtaaaaaatg atttttgata aagacaattt taaagaattt
    10741 gaccaagaac tttggcaggc tattcatgat gaggaaattc gccaacaaaa taatattgaa
    10801 ttaattgcat cagaaaacgt tgtttcaaaa gctgttatgg cagcacaagg ttctgttttg
    10861 acaaataaat atgccgaagg gtatcctagt catcgttatt atggaggaac agattgtgtt
    10921 gatgtagttg aaagtttagc tatagaacga gctaagatgt tatttaatgc tgagtttgct
    10981 aatgttcaac cacattctgg tagtcaggct aatgcagcag cttacatggc tttaatcgag
    11041 ccaggagata ctgtcttagg gatggactta gcagcaggtg ggcacttaac acatggcgct
    11101 tcagttagct tctcaggaaa aacttatcat tttgtttctt actctgttga tcctaagacg
    11161 gagatgctag actatgataa cattttgaaa atagcacagg aaacgcaacc taaattaatt
    11221 gttgcagggg cgtcggccta ttctcgcatt attgattttg aaaagtttcg tcaaatagca
    11281 gacgcagtag atgcttactt aatggttgac atggctcaca ttgcaggttt agttgcttct
    11341 ggtcatcatc ctagtcctat tccttatgct catgtaacga caacgacaac acataagact
    11401 ctacgtggac ctagaggtgg tttgatttta accaatgatg aagcaattgc taaaaaaatt
    11461 aattctgcag tctttccagg actccaaggc ggaccattag aacatgtaat tgctgcaaaa
    11521 gctgtagctt tgaaagaagc tctagatcct tcctttaaaa tatacggtga ggatattatt
    11581 aaaaatgctc aagccatggc taaagttttt aaagaagatg atgactttca tttgatttct
    11641 gatggtactg ataatcattt gttcttagta gacgtcacaa aagttattga gaatggcaaa
    11701 aaggcacaaa atgttcttga agaagtcaat atcaccctta acaagaactc tataccgttt
    11761 gagcgactat caccatttaa aacatcaggt attcgaattg gtactccagc aattactagt
    11821 cgaggaatgg gagttgaaga atctcgaaga atcgctgaat taatgattaa ggcactcaaa
    11881 aatcatgaaa atcaagatgt cctcacagaa gttcgtcaag aaattaaatc attaacggac
    11941 gctttccctt tgtatgagaa ttaatgttat gatggatttt tatatcaaac agattattat
    12001 acatcagttt tctcctaacg acacagagtt agtgctatca gatactccgt taaccctaac
    12061 tccaaggatt gatgactact ttcgtaaaaa attaagtaaa gttttttctg atgaagctaa
    12121 gcgtggctat tttggagagg ataatgtatt tatgagtcat ttacaagatg atttatatgt
    12181 ttcttcctgt caaattgcgc aactttggaa agaagagttt gtcatatcgg aagatcaaaa
    12241 aacgaatgac ttagttttta ttcaatttga caaggatgga atggaacatt ttgctttcct
    12301 tcgaatctca ttaaaggaac aatttgctca tgtttctgaa aatcaagagc agccaattac
    12361 aataacacag aataaccttc caagtgctgc tcaaactcca gatgaagctt tggttgttaa
    12421 caagtcatca aagcaatact atttaattga aaagcgtatc aaacacaatg gttcatttgc
    12481 taattatttt tctgaaaatt tattacaagt acaaccagaa cagtctgtta aaaaatctat
    12541 aaaaatggtt gaacagacag ctcaaaaaat tgctgaaaat ttcaacaaag atgatttttc
    12601 tttccaatca aaaatgaaat cggctattta taagaattta gaagaagagc aagaattgtc
    12661 tccagaaaaa ttagcagatc aattatttga tgataattta acagccaggc tgacttttgt
    12721 tgatgaattg aaagacgcaa tacctggatc tgttcaagta agtgatattg atcattcccg
    12781 tcaaattaag aagttagaga atcagaagct atctctttca aatggtattg aattgattgt
    12841 acccaacaat gtctaccaag atgccgaatc agttgaattt atccaaaacc ctgatggtac
    12901 atactcaata ctaatcaaaa acata
//
LOCUS       DATRME010000054        12654 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRME010000054 DATRME010000000
VERSION     DATRME010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(18..1799)
                     /gene="uvrC"
                     /locus_tag="VBC23_001503"
     CDS             complement(18..1799)
                     /gene="uvrC"
                     /locus_tag="VBC23_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6843444.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            1958..2725
                     /locus_tag="VBC23_001504"
     CDS             1958..2725
                     /locus_tag="VBC23_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6843445.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(2784..4124)
                     /locus_tag="VBC23_001505"
     CDS             complement(2784..4124)
                     /locus_tag="VBC23_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6843446.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(4224..4640)
                     /locus_tag="VBC23_001506"
     CDS             complement(4224..4640)
                     /locus_tag="VBC23_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6843447.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(4644..5141)
                     /locus_tag="VBC23_001507"
     CDS             complement(4644..5141)
                     /locus_tag="VBC23_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6843448.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            5363..5959
                     /locus_tag="VBC23_001508"
     CDS             5363..5959
                     /locus_tag="VBC23_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6843449.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
     gene            6148..7253
                     /locus_tag="VBC23_001509"
     CDS             join(6148..6424,6424..7253)
                     /locus_tag="VBC23_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6843450.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            7477..8609
                     /locus_tag="VBC23_001510"
                     /pseudo
     CDS             7477..8609
                     /locus_tag="VBC23_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8649..8888
                     /locus_tag="VBC23_001511"
                     /pseudo
     CDS             8649..8888
                     /locus_tag="VBC23_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9087..11555)
                     /locus_tag="VBC23_001512"
     CDS             complement(9087..11555)
                     /locus_tag="VBC23_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6843451.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(11568..12488)
                     /gene="lmb"
                     /locus_tag="VBC23_001513"
     CDS             complement(11568..12488)
                     /gene="lmb"
                     /locus_tag="VBC23_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6843452.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         4083 a         2459 c         2115 g         3997 t
ORIGIN      
        1 ctgttctact aagtttttta tttaggagga tcattaagag aatctcggag actttctgca
       61 actgtcctag gaataccaca cataataatg tcctcaatat cagctttttg gatattaggt
      121 aagcttttaa aatgtttcat tagcaattgt ttacgttttg gtcccaaccc agtgatacca
      181 tctaattttg aagaaaagga atttttggag cgtaattgac gatgaaaagt aatagcaaaa
      241 cgatgtacct catcttgaat acggtgaagt aagaaaaatt cttctgaatt acgcggtaat
      301 tctactactt ctaaaggatc tccaaaaagt aattcatgtg tttggtgttt atcattcttt
      361 tgcaaacctg caataggtat ggctaaacct aattggttct caataacatc acgcgctata
      421 tttacttgcc cctgaccacc atcaattaca atcaagtccg gaggtgtcaa gccatctttt
      481 aaaaccctac tataacgccg atgaatgact tctctcatac tagcataatc atctggtccg
      541 ataacagtct taattttaaa ctttcgatag tcttttttac ttggatttcc attcacaaat
      601 accaccatag cagctactgg acttgtccct tgaatattgg aattatcaaa tgcttctatg
      661 cgtactggtt tagggatatt aagaagatta cctaaatttt caattgcccc atgagtttta
      721 cgaatatctt tttccagtaa gtcaaacttc tgttgtaaac tgacacgagc attttttatt
      781 gccagattta ctaactgttt cttttcacca cgttgtggtt taacaatctt acagcctaca
      841 attgtttcaa ccgacttagc atcaatatcc tgtggaataa agacttcttt gggtagaaaa
      901 tgttttgtat cttgataaaa ttgccctatg taagttagaa aatcttcctc tggttcatta
      961 taataaggaa acatattcac gtcacgctga atcaattttc catttcgaac aaaaaagacc
     1021 tgtacacaca tccaaccctt atcaacgaaa taaccaaaaa catctctatc cttcatatct
     1081 tgatgaataa cacgctgctt agtacgcaac aaactaattg cttcgatcaa atctcgatat
     1141 tcagccgcac gctcaaattc catagtcata gcagcgctct tcattttttc agttaatccg
     1201 ttaacaattt tattatcttt accattaaga aactgtttta catcttctct cagactatcc
     1261 caataagctt tattagtttg acaaactgta tgtgcattgc attgccctaa atgataataa
     1321 aagcatactt tatttgctgg attagtacat ttcttaaaag gaaagagcct atctagtaac
     1381 cgcttaatct cagttgctgc cccagaatcc ggataaggtc caaaataagt gccatctgat
     1441 ttttttactt gacgtgtaat cataagacga ggataacgtt catttgttat tttgatatat
     1501 gggtaagact tatcatcttt caatcgaata ttatacttcg gcatattttc ttggattaaa
     1561 ttaatttcca ataatagagc ttcggtatta gatgttgtca caatgtattc aaagtcttca
     1621 atctcagaga ccaaaagttc tgtcttagta ttatgactcc catgaaaata acttttcaca
     1681 cgattcttaa gatttttagc cttacctaca taaataattg tcccattctt atctttatga
     1741 aggtaacaac caggactgtc tggtaacaat tctagcttat gtttaatcag ttcattcata
     1801 gaactattat aacaaaatgt actgtaatct ttacgaaata aatactattc cccaacatct
     1861 attgtatctt ccaatacttg aataaaaccc caatactcct agtctaagca attattttat
     1921 gttacacttt aaacaagtga attattgagg agtttttatg atagacgttg agaatattct
     1981 tgaaaaaatg aaacctaacc aaaaaatcaa ttacgattgg gtcatgcaac aaatggttaa
     2041 gcagtggcaa gctagtgata tccgtccgaa aattctgatg catgtttgct gtgctccttg
     2101 tagtacatat acccttgaat acttatcaca atgggcagat gttaccatct attttgctaa
     2161 ttcaaatatc catcctaaag atgaatatta tcgccgcgaa tatgttactc aaaaatttgt
     2221 tcatgatttc aacaaaaata ctggatattc tgtacaattc ttatcagcac catatgaacc
     2281 aaatgaattt ttcaaaattg ttcatgggtt agaagaagaa cctgaaggtg gggatcgttg
     2341 taaagtttgc tatgatttcc gtcttgataa gacagcggaa aaagccgttg aactaggttt
     2401 tgattacttt ggcagtgccc taactattag tcctcacaaa aactctcaaa caatcaatac
     2461 tattggtatt gatgtccaaa aaatttatga tactcagtat cttcctagcg accttaaaaa
     2521 aaataaaggt taccaacgtt ccgttgagat gtgtaaagat tatgatatct accgacaatg
     2581 ttactgtggg tgtatcttcg gtgctaaaga tcaagggata aatcttctcc aaatcaaaaa
     2641 agatgcgaaa gctttcgtga gtgataaaga cggcaaagaa gaatttccta atatacgctt
     2701 cacatttaat ggcaagtcaa tgtaaatgat aaaaaaagac ttagtaaaga actaagcctt
     2761 tttggtataa atcttgtcaa actttaataa tacaacttct taatcttttg cagacgttgg
     2821 tttagaaata gaccacaaac aaaaatacct gtgattaaac caatccaata ggcaaacgga
     2881 cctaactggc tatttttttc taagataacc gctaatggca aagcacataa ccaataagag
     2941 cccgcaccga tcataaatgg ttttgttgta tccttatagc ctcgtaaaat cccctgtaca
     3001 ggagctgcat aagcatctgc aaactggaaa aagagactat aagttaggaa ttgagctgta
     3061 atagcgacaa agtgagggtc actattatac attgctgcta cattctcacg aaatagaaat
     3121 aaaaatagta aggttcctga tgtaatccct accgctgtta agcgtcctat cctactataa
     3181 gtgtttgcgt cctgaaagcg ctctgcccct acttcaaacg atattgtaat agctagagca
     3241 gtggaaatac ttaaaggaaa tgcatacatt aatgatgaaa aattcatagc agcctgatgt
     3301 gctgcaataa tgattgaaga aaattttgcc atgaataagc ctactactgc aaaaattgca
     3361 acttctgcaa aaatttgtaa accaatcggt aatcccaatc gaatatcttc aataatcaaa
     3421 ggagctttta ttctttccag agtccatata tgatatgttt taatttgagg atgaagtgac
     3481 atcacaataa taataacaat aaaaatagcc caataagtta aagaagttcc aagacctgcc
     3541 cccgcacctc ctagtctagg cataccaaat ttaccgtaga taagcatata attaaaaaat
     3601 gaattaaagg gtagaattaa aagcatcaga tacatagata accttgttaa ccccaatgca
     3661 tcaaagaatg aacggcaaat gctaaacaac accagcggca tgattccaat caacatataa
     3721 tttaaataac cacgaccaac tgctagaact tcatcttcta aacccaaact ccccaagaca
     3781 ggttgagcaa taaattgcat gattaaaaat aatattaagg acagtatcaa acctaaatat
     3841 agaaattgat gaaattctgt gcgaatttgt tctttatttc ctctacccaa atgttgacca
     3901 actactggta ctaatgctga aatcatacct actaataaag cgaaaaacgg agtccataaa
     3961 ttactagcag ttgacacacc tgccaaatgt agctgactat actgtccagt cataaccgaa
     4021 tcaataaaag tagctgaaaa attagcaaat tgataaatca atactgggat aaataatttc
     4081 aaaaataaaa caaatttttc cttatttgtc tgagtttgat acatcttttt tctccttttc
     4141 accttaatca atttatttta gcttagctag gatactctgt caatgctatt taaaaaggtt
     4201 gagaaataat cctcaacccc attttagcta agctcagtat tgatttgcca tgtcatacca
     4261 tatttgtcag tcacatttcc ataagctgga ctccacggaa ctgcttgtag aggcatttta
     4321 atgttcattg catctcgaga aagcttattg aataactctt gcgcttcttc tacagattcc
     4381 acaattaaag ctgctgtcat attttgtcca atttgaaaat caaaaatagg attattgtca
     4441 gaaagttgca ttcgaatacc atcaatatta aattgagcgt tcataattaa atgttcaagt
     4501 tctggcggac aatctggcca accctgtgca aaagttgtga cgtgttctgc ttttgtatta
     4561 aaagcctctt cataaaatgt aatagcttcg gccccacgac cattaatatt catataaatt
     4621 tcaattgctt tcattaccat acctcacttc atattatcca accaacgctg catcagagtt
     4681 tcattttccc cttttagggt aaaataagaa ccacgtatta tacctgcgag ttttgacttt
     4741 tgacgagcaa acttaaactt gtccgctaat tcaataggaa tatccattcc atctgataat
     4801 tttaaaccta cagctctctt tttaggatta tctaattcat ataaaatatt aatggctaaa
     4861 ccatcctcat agaaaacatg tgtccataaa atacgattct ctaaaaaaag ctgtgaaatc
     4921 tctaaaggct ttgcagcaaa aacaaggtta cgttcatttt ttagaaggtg attaatatct
     4981 gccatcaatt ctggatagtc tgatactgga aatgtttcgt aggctacttt gaatttattc
     5041 cagaaatagc gagcttcatt tgcacgcaaa ccagccaagg cttctgccac tggagaagtc
     5101 tccaaacctt tagttgaaac ggttttaaag tcgataatca tgttctttcc tttttgatta
     5161 aaatcttcga taatttagta tactcttttt tgaagaaaaa gtacattcta atcacttata
     5221 aaaagtagta catttagaac actaccacct cagtaatttc ttgacctttc tctacttaat
     5281 acttataatt aatctagact taatgtcaaa gcatcaatca aggtgtaaac ctgattcacc
     5341 taaaaagctg atggagctaa acatgaccaa aaagatattc ttatttatct gcgctactct
     5401 tttagtagga atagccttat atacaccaac aacttctatt tttaatcata acaacgttta
     5461 tgctgccacc aagaaagcaa aaattaaatt taataaaact caaaaaaaaa ttgtcaaaaa
     5521 agcaagagaa tatgctaagt ctggccatat gtctaaagat agtatcattg aaaaattaaa
     5581 aaaggattct aagaaatatc gtcaagaaga catcaacttt gtgattaata acctcaaggt
     5641 tgattacaaa aaaaatgcct tgatttctgc caaaatttat tcaaagacca tgaatttatc
     5701 taaacaatct atctttgaac agctctattc ggagtctcca gataaggcaa cccactctga
     5761 taaatttaca aaagaagaat cccaatatgc tatagaccat ctcaaagtag atttcaaaga
     5821 aaatgctctt gagactgcta aaagttatca aagtagttct tctttatcca aagaagaaat
     5881 ctataaacaa ttgacatcta cgctgggcga taaattcaca aatgatgaag cccaatatgc
     5941 tgtagatcat ttgaaatgat gacataccaa tagtctatcc ttatgtaata gaattgatag
     6001 cttatgctaa tattccagct gtaaaatagc aagaaccctc ctaagttcat taagacttag
     6061 gagggttttt atcaatatga ataggttaca ctaaactaga cagaaattat aaagtgttct
     6121 acacttaaga aaaactggag aaaaaccatg tctagaaaag tacgtcgtca cttcaccgat
     6181 gattttaagc aacaaattgt tgacctttac aatgttggta gaaagcgtag cagtctcatc
     6241 aaggtatatg agctaacccc ttccaccttc gataagtggg ttagacaagc caaaacaact
     6301 ggttcattca agtctgttga taatctgaca gatgaacagc gggagctaat tgaactcaga
     6361 aaacacaata aagaactcga aatgcaatta gatatcctaa agcaagcagc ggtgattatg
     6421 gcacaaaaag ggaaataatc actgctaata aggataaata cagcatttca aagatgtgtc
     6481 gctggctgaa tatgccacac tcaagttatt actatcaagc cgtggagtca gtatctgaaa
     6541 cggagtttga agaaactatt aaaagaattt tcctcgatag cgagtccaga tacggatcca
     6601 gaaaaatcaa aatatgcttg aataacgaag gtatcacact ttcacgtcgt cggattcgac
     6661 gcattatgaa gcgactcaat ttggtttctg tttatcagaa agccaccttc aaaccacatt
     6721 ctagaggcaa gaatgaagcc cctattccca accacttaga caggcaattt aagcaagaaa
     6781 gaccactaca ggccttagtc actgacttaa cctatgttcg tgtaggcaat cgttgggctt
     6841 atgtttgcct catcattgac ctatacaacc gtgaaatcat cggcctgtct cttggttggc
     6901 acaagaccgc tgaactcgtt aagcaagcca tacaaagcat cccttacgcc ctgaccaaag
     6961 tcaagatgtt ccattcagat cgtggcaaag agtttgataa tcagttaatt gatgaaatat
     7021 tggaagcctt tggaatcaca cgttcgctta gtcaggctgg ttgtccttat gacaatgccg
     7081 tagctgaaag tacgtatcgt gctttcaaaa ttgaatttgt ttatcaagaa acctttcaat
     7141 tgctggaaga actagctctt aagactaaag actatgttca ttggtggaat taccaccgca
     7201 ttcatggtag tctcaactac caaacaccaa tgactaaaag attaattgcc tagaaaaaca
     7261 ctttataaat gttgtacaga aaagtgttgc cttttcactt aataaaagaa agattgaagg
     7321 aacacttctc tttcattccg accaagggtc acaatttaag tccggggaat ttagaaaaat
     7381 aattgatgac aacaataggc actgaatccc taaaatgaga caaaagaaaa acactcctgt
     7441 tgaaatttag taaactagaa acaggagtgt tttattatgg ttaaaaaagc atattcatgg
     7501 gaaactaagc tggcttgcat cgatatgaag aaagcaggca agtctaacag agttattatg
     7561 gagactttag gcatcaaaaa taatagtcaa atctatactt ggatgaaatg gtacgaaaac
     7621 gaggagcttt atcgttttca tcaaggtgtt ggcaagcaat atacctacgg taaaggcttg
     7681 gaacatctgt ctgaagtgga acaattacaa ttacaggttg acctcttaaa aaagtatcga
     7741 ggcttgataa ggaaatcgat aaagtaagtc ttatcaagct tgtggaagac tataaaaaca
     7801 actacccgat agttttcatt ctagactgtt ttaaggtcaa gcggtcgacc tattaccgtt
     7861 ggaaaaagga gtatgagaag ccgcaaaaaa agatgacctt attgaattaa ttgaacagct
     7921 ctgtatggag aatcacttta tttatggcta tcgtaccatt acacgattgc tcaaaaaaat
     7981 ccacggattg actgttaata caaagaaagt ctatcgtatc atgaaaaata atggctggct
     8041 ttgtcggaca cgtaccaaga aagttcccaa tttgggtaaa gcttattatt taacagataa
     8101 taaattgagt cgggatttcc atgccgataa gcctaaggaa aagctcgtaa cagatattac
     8161 ctacctatat ttcggtaact gtaaattgta tctctcatca attatggatc tctataaccg
     8221 agaaattata gcttacacta tctcagattg tcaggacacg gattttgtac tagacaccct
     8281 taatcagtta aaattgccta aataaaggag caatcttaca cagtgaccaa ggctcagtgt
     8341 atacttctat ggcttactac caggcttgca cggaaaaaga cattatccgc tcaatgtctc
     8401 gaaaaggaac acctgcagat aatgcctgta ttgaatggtt tcacaccgtc ctaaagactg
     8461 aaacctttta tttccataat aggagaaaat acaacaaaga tagtataaca aatattgtta
     8521 aaaattacat tacattttat aatgaaacta gaattcaaca gagattaaac gaccagtcgc
     8581 ctgtacagta cagaaaactg attgcctaaa agtgtttttc ttgcgtctca ctattagggt
     8641 gcagtgccaa tatcatgcat tctttttcta atcctggata tccttatgat aatgccgtaa
     8701 ctgaagcatt tttcaagtat ttaaagcata gacaaatcaa ccgaaaacat tatcaaaata
     8761 tcaaacaggt tcaattagac tgctttgaat acattgagaa tttttataac aattacaacc
     8821 cacatacggc taatctagga ctaaccccta atcagaaaga agaaaattat tttaactcaa
     8881 taaaataaca cagttttctg tctacttatt tgacattagt ccaagaatcc tcctcttttt
     8941 ttactattct aacatggata ccatttcttt gcaaaagaaa accgctatta tccaactttt
     9001 atcactactc gttacataga atgacttcaa aatcaccgta acttgctttg acaaagcagt
     9061 gctttaacaa tgagatcttc ttttggttaa gggtttattt gttgaagtgt cttgatatca
     9121 taagttacca attcaccatt tttattataa aattggacac cttctggttg gaaaatgaga
     9181 tacttaggat cgatattagc tttttgtgct aattgattga tatgatcttc tagggttgct
     9241 ctatctagac catagtctgg taaactgtct ataaagtcat ctgattcttt ttcttcttta
     9301 ctggcttcac ttggctgttg gttttcattg ctcttatctg cctgttgctt ttcttcaggt
     9361 ttatccgtat cagtagcatc accagcattt ttctttgcca ataactcttg agcttgttgc
     9421 cactcagctt gggatagatc ggatttatta atggttctca atgaactgcc atctgctctt
     9481 gggatgctaa aggagccatc tttccataca aaagtttctt ttgccaaaac atctcgtgga
     9541 tcgaaaatat agccgtctgg tgctgcaaaa cgaccttctg ctagggcttt ttgaacttct
     9601 tcagcagaat ggataatttg ccagtttggc ataccagcac gtttatcaag aggcgtaaca
     9661 tttggaatga ccgaacctga ctcttcatgc cctggcttag accatacatc cggacgaact
     9721 tcggggtgtt gcatcacata cttgattgtt gcaatctgat tgcgcgtcaa ccatgaatac
     9781 ggaacgacat ggatatgatc aatatgtggg ataacaaacg aacttccagt atcgtaagta
     9841 gcattaccag catgcatcgg cagacttgac acatctacag caagtcgtgg ctcaatgcct
     9901 gtatcaacaa tgtcataacg gtaatgcttc ttatctttaa gcattagttc ttgttcggca
     9961 aaggcaatct gagtcaaatc aagttgataa cgagcataga aatagtcctt gccatctttt
    10021 ggcataatat agcccacttt accatctttt gttactttgc gactcacttt tttagtgtca
    10081 aagagtggtt tttctttgcc ttgttcctga tccaaagcag caacaagctc atcagcttga
    10141 ccttttgctt tcacccagtt agcgacctca tccaactcat attgttcaag ttctccaaat
    10201 cctatatagt ggaaatgatc tccgtgttta gctgtaactc ctgatttatc cactgaatga
    10261 atggattctt tactaaaaac ataagcatca ctcgtatcat atggtttacc atctaagcct
    10321 tttccgtaag ctttgatgcg atgaccaaga aaggtatgtg tcacttcttt atctgatggt
    10381 tttgagtgat ctgaacctga gtcgttgtca tcagtttggc cggctaagta tcgatctgct
    10441 aattccattt caagaggtga taactgactt cttgggataa tatgataatg atctccatga
    10501 ggcaccacat acccaaaagc gtttgatttg atcacttgag tcggttcaaa aatcaaccca
    10561 tcttcttcca catgacggta tttcaaatca agacggtgta gttgatctaa aagttcctta
    10621 aaggtttttc ctttaaactc atctctttgg tgtttgtttt gtgacgcatc atttggccta
    10681 ggaggcgctg gatgataacc accgttatct ggctgatgac cttgtccagg gttaggcgtc
    10741 acatcaggaa ttggggcttt cctacgacct ggggctggtg tcgggcggta atcagacggt
    10801 ctagcacctc gaccttgttt ttgactccag taggcttgtg cagcagctag ctcacttgga
    10861 gacaaatctt ttttaggaat ataatgatag tgattaccat gaggtactaa ataagcatct
    10921 cctaaatcat caatgatatc tgtcggacta aaaatatagc catcgtctgt agtatagcgt
    10981 ccttgtcttt ttgcttcatt gactgccgca acttcttctt tactgagatg ggccacttga
    11041 gctaaacctt tttctttagc ttctttagtt cctttggcta cttgctcagc aatttgttgt
    11101 ttggttcgaa tgtttttgcg cttactacct ggcttgaggt aaacataata gttgccattg
    11161 actttaataa cgtaaccgtc taagatttca ttgataacgt ctgattgttt aaaatggtaa
    11221 ttaggatccg tcatcaacaa ctcttcacta ataatcgcat cataaggaac tttcccattg
    11281 taaaaatgat aatggtcacc gtgtgaggta acataacctt ggtcagtaat tttgactacg
    11341 atctgttcag cagagatgcc ttcttcagca ctgatttgat ccatcgtttt gtttgtttta
    11401 ggggctttta ccttaccttt gctatcatca atataggcaa tctgattgtc ctttgttgct
    11461 agacccatat gatgcttacc aagctggtaa cttccaatat gagtagctag taaaatagca
    11521 gcaactgagc cgatataacc atatgttttc ttcactaata atctccttta cttcaactgt
    11581 tgatagagca cttccaaatt tgctctaaga ttttctagat atgtcttgtt tccgcttgga
    11641 gcagcttcaa gtggacttaa tgtctttact ttagctcctg ttgatttcgc aatagcatga
    11701 gcaattttgg ggttgacgtt gtcttctgca aaaatagtct tgacgttgta ttctttaaca
    11761 aagtcttgaa tttctttcaa ttggcgagga gagggctctt gctctggaga aatacccgag
    11821 ataccaagtt gtttcaagcc gaatcgttta gccagataag aaaatgccgt gtgttgcgtc
    11881 acaaatgttt ttgagcgcac ctttttaaat ttttgagtgt attcttcagt tagttgctct
    11941 gcttcttttt tgaaagcctt agccttttta gtgtaactgt ctttgtgttt aggatccaaa
    12001 tgtcctagct ctttagcgat attaacagct tcctcaccag ctaaaacggg atccgtccag
    12061 gtatgtgggt cataaagtgt cgcagggtca atgccttgtg tgacttccat atcttctagc
    12121 cctttgactc tatctagtgt cagaggtttt gacgcttcaa acacattaac ctttgatttt
    12181 tttaaattag ggtctagatc ccttgcccaa gcttctaagg tatgtgaatg gtaaacaaac
    12241 aaatccgcgt cataaatagc tgccacatca tttacagacg gttcaaagga atgaatgcct
    12301 gcacctgatt ggatcatcct cacatcattt aggtctccag atacttcttt tgtcatcgca
    12361 tacattgggt aaaagctggt tacaactgac atgccttgcg taggctgttt ggggtttgct
    12421 gacttatcac accctgctat cattactaaa ctcacaacca tagccatgag aaaaaaaact
    12481 tttttcatag tacctcctca attataattt aaccagttat taaccagtta attttactcc
    12541 tttatcaatg ctttgtcaat gacaactacc atcttttatc acatcaactg aagggataag
    12601 caaaaggcaa ttgttgacag ctatttgtcc taacttcttt ttgatgaggt agca
//
LOCUS       DATRME010000055        12621 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRME010000055 DATRME010000000
VERSION     DATRME010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            12..3712
                     /locus_tag="VBC23_001514"
                     /pseudo
     CDS             12..3712
                     /locus_tag="VBC23_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            3922..4830
                     /locus_tag="VBC23_001515"
     CDS             3922..4830
                     /locus_tag="VBC23_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6843453.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            5384..6394
                     /gene="nrdF"
                     /locus_tag="VBC23_001516"
     CDS             5384..6394
                     /gene="nrdF"
                     /locus_tag="VBC23_001516"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6843454.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            6395..6808
                     /gene="nrdI"
                     /locus_tag="VBC23_001517"
     CDS             6395..6808
                     /gene="nrdI"
                     /locus_tag="VBC23_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6843455.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            6810..8975
                     /gene="nrdE"
                     /locus_tag="VBC23_001518"
     CDS             6810..8975
                     /gene="nrdE"
                     /locus_tag="VBC23_001518"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6843456.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            9038..12205
                     /locus_tag="VBC23_001519"
     CDS             9038..12205
                     /locus_tag="VBC23_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6843457.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPGKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQVEKNLKPVDYKTPIFN
                     KDLPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            12218..12607
                     /locus_tag="VBC23_001520"
     CDS             12218..12607
                     /locus_tag="VBC23_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6843458.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         4358 a         1961 c         2474 g         3828 t
ORIGIN      
        1 aaataggaaa agtggataaa catcactcaa aaaaggctat tttaaagtta acacttataa
       61 caactagtat tttattaatg catagcaatc aagtgaatgc agaggagcaa gaattaaaaa
      121 accaagagca atcacctgta attgctaatg ttgctcaaca gccatcgcca tcggtaacta
      181 ctaatattgt tgaaaaaaca tctgtaacag ctgcttctgc tagtaataca gtgaaagaaa
      241 tgggtgatac atctgtaaaa aatgacaaaa cagaagatga attattagaa gagttatcta
      301 aaaaccttga tacgtctaat ttgggggctg atcttgaaga agaatatccc tctaaaccag
      361 agacaaccaa caataaagaa agcaatgtag taacaaatgc ttcaactgca atagcacaga
      421 aagttccctc agcatatgaa gaggtgaagt cagaaagcaa gtcatcgctt gctgttcttg
      481 atacatctaa aataacaaaa ttacaagcca caacccaaag aggaaaggga aatgtagtag
      541 ctattattga tactggcttt gatattaacc atgatatttt tcgtttagat agcccaaaag
      601 atgataagca cagctttaaa actaaggcag aatttgagga attaaaagca aaacataata
      661 tcacttatgg gaaatgggtt aacgataaga ttgtttttgc acataactac gccaacaata
      721 cagaaacggt ggctgatatt gcagcagcta tgaaagatgg ttatgggtca gaagcaaaga
      781 atattttgca tggtacacac gttgctggta tttttgtagg taatagtaaa cgtccagcaa
      841 tcaatagtct tcttttagaa ggtgcagcgc caaatgctca agtcttatta atgcgtattc
      901 cagataaaat tgattcggac aaatttggag aagcatatgc taaagcaatc atagacgctg
      961 ttaatctagg agcaaaaacg attaatatga gcctgggaaa aacggctgat tctttaattg
     1021 ctctcaatga taaagttaaa ttagcactta aattagcttc tgagaagggc gttgcagttg
     1081 ttgtggctgc cggaaatgaa ggtgcatttg gtatggatta tagcaaacca ttatcaacta
     1141 atcctgacta cggtacggtt aatagtccag ctatttctga agatactttg agtgttgcta
     1201 gctatgaatc acttaaaact atcagtgagg tcgttgaaac aactattgaa ggtaagttag
     1261 ttaagttgcc gattgtgact tctaaacctt ttgacaaagg taaggcctac gatgtggttt
     1321 atgccaatta tggtgcaaaa aagattttga aggtaaggac tttaaaggta agattgcatt
     1381 aattgagcgt ggtggtggac ttgattttat gactaaaatc actcatgcta caaatgcagg
     1441 tgttgttggt atcgttattt ttaacgatca agaaaaacgt ggaaattttc taattcctta
     1501 ccgtgaatta cctgtggggg ttattagtaa agtagatggc gagcgtataa aaaatacttc
     1561 aagtcagtta acatttaacc agagttttga agtagttgat agccaaggtg gcaatcgtat
     1621 gctggaacaa tcaagttggg gcgtgacagc tgaaggagca atcaagcctg atgtaacagc
     1681 ttctggcttt gaaatttatt cttcaaccta taataatcaa tactaaacaa tgtctggtac
     1741 aagtatggct tcaccacatg ttgcaggatt aatgacaatg cttcaaagtc atttggctga
     1801 gaaatataaa gggatgaatt tagattctaa aaaattgcta gaattgtcta aaaacatcct
     1861 catgagctca gcaacagcat tatatagtga agaggataag gcgttttatt caccacgtca
     1921 gcaaggtgca ggtgtagttg atgctgaaaa agctatccaa gctcaatatt atgttactgg
     1981 aaacgatggc aaagttaaaa ttaatctcaa acgagaggga gataaatttg atatcacagt
     2041 tacaattcat aaacttgtag aaggtgtcaa agaattgtat tatcaagcta atgtagcaac
     2101 agaacaagta aataaaggta aatttgccct taaaccacaa gccttgctag atactaattg
     2161 gcagaaagta attcttcgtg ataaagaaac acaagttcga tttactattg atgctagtca
     2221 atttagtcag aaattaaaag aacagatggc aaatggttat ttcttagaag gttttgtacg
     2281 ttttaaagaa gccaaggata gtaatcagga gttaatgagt attccttttg taggatttaa
     2341 tggtgatttt gcgaacttac aagcacttga aacaccgatt tataagacgc tttctaaagg
     2401 tagtttctac tataaaccaa atgatacaac tcataaagac caattggagt acaatgaatc
     2461 agctcctttt gaaagcaaca actatactgc cttgttaaca caatcagcgt cttggggcta
     2521 tgttgattat gtcaaaaatg gtggggagtt agaattagca ccggagagtc caaaaagaat
     2581 tattttagga acttttgaga ataaggttga ggataaaaca attcatcttt tggaaagaga
     2641 tgcagcgaat aatccatatt ttgccatttc tccaaataaa gatggaaata gggacgaaat
     2701 cactccccag gcaactttct taagaaatgt taaggatatt tctgctcaag ttctagatca
     2761 aaatggaaat gttatttggc aaagtaaggt tttaccatct tatcgtaaaa atttccataa
     2821 taatccaaag caaagtgatg gtcattatcg tatggatgct cttcagtgga gtggtttaga
     2881 taaggatggc aaagttgtag cagatggttt ttatacttat cgcttacgtt acacaccagt
     2941 agcagaagga gcaaatagtc aggagtcaga ctttaaagtt caagtaagta ctaagtcacc
     3001 aaatcttcct tcacgagctc agtttgatga aactaatcga acattaagct tagccatgcc
     3061 taaggaaagt agttatgttc ctacatatcg tttacaatta gttttatctc atgttgtaaa
     3121 agatgaagaa tatggggatg agacttctta ccattatttc catatagatc aagaaggtaa
     3181 agtgacactt cctaaaacgg ttaagatagg agagagtgag gttgcggtag accctaaggc
     3241 cttgacactt gttgtggaag ataaagctgg taattttgca acggtaaaat tgtctgacct
     3301 cttgaataag gcagtagtat cagagaaaga aaacgctata gtaatttcta acagtttcaa
     3361 atattttgat aacttgaaga aagaacctat gtttatttct aaagaaggaa aagtagtaaa
     3421 caagaatcta gaagaaataa cattagttaa gcctcaaact acagttacta ctcaatcatt
     3481 gtctaaagaa ataactaaat caggaaatga gaaagtcctc acttctacaa acaataatag
     3541 tagcagagta gctaagatca tatcacctaa acataacggg gattctgtta accatacctt
     3601 acctagtaca tcagatagag caacgaatgg tctatttgtt ggcactttgg cattgttatc
     3661 tagtttactt ctttacttga aacccaaaaa gactaaaaat aatagtaaat aaaactaagt
     3721 aaactactcg gtaatatgat gaaaagttca gtttaacatt atgttaagct ggacttttag
     3781 ggtattgact cttatttttg attaatttta tattgattaa cggcatataa tattataata
     3841 ttataattac gattagtatg tgatactgtt cgctttgtaa gctttcataa gatgaactag
     3901 ccttaaggag ttttgatagc aatgaaaacg ataaaagatt ggcaaataaa tatttgctta
     3961 gcgacctata atggtcaaaa ataccttcgc cagcaattag atagcataat acaacaaggt
     4021 tatactgact ggatttgttt aataagggac gatggatcta ctgatgatac agtagctatt
     4081 attaaagaat atgttaatag agattcacgt tttatattta ttaattctaa cgatgataga
     4141 aagttaggaa gtcaccgcag tttttacgag ttggttaact ataaaaaagc agatttttat
     4201 gttttctcag accaggatga tgtttggaaa gaaaatcgtt tagaacgtta ccttgaagaa
     4261 gcagagaaat ttaaccaaga attaccattg cttgtttatt cgaactggac ttctgtagat
     4321 gaaaaattaa cagtattgaa agaacataat ccagcaacag ttattcaaga acaaattgcc
     4381 tttaaccaaa taaatggtat ggttatcatg atgaatcatg agttagcaaa gctttgggaa
     4441 taccgtcaaa ttggcgccca tgattcttat gttggaactc tggcttacgc cgttggaaat
     4501 gtggcttata tttctgacag tacagtcctt tggcgtagac aagtaggggc agagtcgcta
     4561 aataattacg ggagacaata tggggtggct acgttttggc agatgataaa tacttctttt
     4621 gatcgtgctt cgcttatttt tgctcaagta tccgataaaa tgtccctgga gcgtaaactc
     4681 ttctttagtc gttttataga attaaaaaat gccaatctaa taagaaggat ttatctttta
     4741 tcaaaattaa aacttcgtcg gaaatcattg aaagaaacag ttgctatgac tatcctactt
     4801 ttaacaggtt acggtaaacc aaaagcataa tttttttggt tgaaggaaat caagttgagt
     4861 tgtagttaaa aatatagaaa atatttattt ttcaaaaagt tgttgacact taacgaaatt
     4921 agtgtataat aatattaaag aacaaggtag aaccttggct cgcattaatt tcgggttttg
     4981 gaaaaggagt agcaatcgct actccttttt tgttttacac aggaaaagta tgaaggaaaa
     5041 gccttttttc ttataaaaat taagcttgct taatttttta atttttatat tctttacatt
     5101 atgatgaaaa cacaagatat agtggtgata tatgttgaaa gacacaaaac tttgtgttag
     5161 tatactgttt aagcagatcc ttaataaagg taaccgacaa aaaagtttaa cgtgacttaa
     5221 caaaacagtg gttttgaagc aagagttagc ttcttaccag aataaaaaaa catcaagaag
     5281 aacattccaa tagttgtgtg attttaacaa aaatccagtc ctcttgggag gcttttttgg
     5341 tgcttaaaac tcgcttaatt ttaatattta ggagactagt ttaatgcaat catactatga
     5401 tcgttctcaa tcaccacttg actatgcctt gtcagaaaaa gcttttccta tgcgttctgt
     5461 taattggaat aagctaaacg atgacaaaga cttagaggta tggaacagag taactcaaaa
     5521 tttctggtta cctgaaaaga tcccagtttc taatgacctt aattcttgga gaacattgga
     5581 tgctgattgg cagcaattaa ttactcggac ttttactggt ctaaccttat tagattctgt
     5641 gcaggctact gttggtgata ttgctcagat taagcattct cagacagatc atgaacaagt
     5701 tatttatgct aactttgcct ttatggtagc tattcatgcg cgatcttatg gaacgatttt
     5761 ttcaacctta tgcactagcc aacaaattga ggaagcacat gaatgggtag tggatactga
     5821 aagtctacaa gctcgatcac gtatacttat tcctttttat actggtgacg atcctttgaa
     5881 atctaaggtt gctgcagcca tgatgccagg atttttgtta tatggtggct tttaccttcc
     5941 tttttacctt tcagccagag gaaaattacc aaatacgtct gatattattc gtttaatttt
     6001 acgagataag gtaattcata actattacag tggctataag tatcaacaga aagttgctaa
     6061 attatctgta gaaaagcaag ccgaaatgaa gacatttgtc tttgacctcc tctatcagtt
     6121 gattgattta gaaaaagcct atctttacga actatatgat ggttttgatt tggctgaaga
     6181 tgcaatcaga tttagcattt acaatgctgg taaattttta caaaatttag gatatgattc
     6241 tccatttaca gaagaagaga ctcgtatatc tcctgaggtt tttgcacaac tttcggcgcg
     6301 tgcagatgaa aatcatgatt tcttctcagg aaacggctct tcatatatta tggggattac
     6361 agaagaaaca ttggatgaag attgggagtt ttaaatggat agtacagtga aggtggtcta
     6421 tttttcatcg aaatctaaca atacccatcg ttttgttcaa aagttggctt gttctaacca
     6481 gcgtattcca tcagatggta gtagtatatt ggttactgag gattatattt tgattgtgcc
     6541 tacttacgct ggaggaggag atgataccaa aggcgcagtt ccaaaacaag ttgtacagtt
     6601 tttaaatgtt cgacaaaatc gggaacattg ccaaggggtt atttcttctg gaaataccaa
     6661 ttttggtgac acttatgcca ttgctggacc tattattgct cgaaaattaa atgttccttt
     6721 gcttcatcaa tttgaattac tgggaacaca agaagatgtt acaagggtaa aggagttact
     6781 ttgccaattc actagaaagg ataaataaga tgacacaatc agatgcatat ctctcgttga
     6841 acgcgaagac acgctttaga gatcgcacag gtaattatca ttttacttcg gataaagagg
     6901 ctgttgaaca atatatgata gaacatgttg aacctaatac gatggtgttc acatcactaa
     6961 ttgaaaagct agattatttg gtttctaata actactatga atcggacctt ctaaaacaat
     7021 ataaccttga gtttatttgc caaatttttg agcatgcata cgctaagaaa tttgcttttc
     7081 taaattttat gggggcttta aaattttata atgcttatgc tcttaaaaca gaagacaatc
     7141 gttattattt ggaacactat gaagatcgtg ttgtaatgaa tgctctgttt ttagctgctg
     7201 gtgatgaaaa agctgcctat gacctagtag atgacatgct agcaaatcgc tttcaaccag
     7261 caacacctac ctttttaaat gcagggaaaa aacgtcgtgg tgaatatatc tcttgctatt
     7321 tgttgcgcat tgaagacaat atggaatcta tttctcgtgc tattagtaca agccttcagt
     7381 tatcaaaacg aggtggtggg gtagcacttt gcttaaccaa cttacgggaa tttggtgctc
     7441 caataaaagg tattaaaaat caagctactg gtattgtacc tgtcatgaaa ttactagaag
     7501 attcgttttc ttacgccaat caattaggac aaagacaagg agctggtgca gtttacttac
     7561 acgctcacca tcctgaagta ttaacgtttt tagatactaa acgtgagaat gccgatgaga
     7621 aaattcgaat taaatctctt tccctaggac ttgttattcc tgatattact tttgaattgg
     7681 ctaaggcaaa taaagatatg gccttgtttt caccgtatga cattgagcga gtatatggta
     7741 aaccaatgtc tgatatctct atcacagaag aatatgaaac tttattagcc aatgcggata
     7801 ttcgtaaaac ctttattagt gcacgaaaac tatttcaaac gatagcagag cttcattttg
     7861 aatctggtta tccttatatc ttatttgaag atactgtaaa tgctaaaaac cctcacaaaa
     7921 aagagggacg aattgttatg tctaatttat gttctgagat tgcacaagtt aatacagcta
     7981 gtcaattttc agaagacctg actttcacta aggttggaca tgatgtttgt tgtaatttag
     8041 gttctattaa tattgcgcgt gctatggatc aagcggcgga ttttgaaaaa ttaattgcta
     8101 atagcattag agcccttgat cgtgtatctc gaacttctga tttagatagt gctccatcta
     8161 ttaaaaaagg taatgcagct aatcatgctg ttggtttagg tgctatgaat ttgcatggat
     8221 ttttggcgac taaccatatt tattacgatt ctcaagaggc tattgatttt acagattgct
     8281 tcttttacgc tatggcttat tatgccttta aagcttctaa ccatttagca aaagaaaaag
     8341 gaacctttga gggcttctcg gaatcaagtt atgctgatgg cagttatttc tatcaatata
     8401 ccgaacaaaa ctttgagcca aaaacacagc gtgttaaaaa tctactagct gagtatggtc
     8461 tgactctacc aagccaagag gattggagaa agcttgtgca aagtattaaa gagataggat
     8521 tagctaatgc gcatttatta gctgttgctc cgacagggtc aatcagttat ctttcttctt
     8581 gtactccgag ccttcaaccg gttgtatcac ctgtcgaagt acgcaaggaa ggagcactgg
     8641 ggagggttta tgtacctgct tataagattg atgcagataa ttatgtctac tacaaaaaag
     8701 gagcttatga agtgggatct gaggcgatta tcaatattgc agctgccgct caaaaacaca
     8761 ttgatcaagc tatttcgtta acgcttttca tgacagatca agcaactacg cgagatttaa
     8821 ataaagccta tattcaagca tttaaacaaa aatgtgcctc tatttattat gtacgagtga
     8881 gacaggacat cctagaaggt agcgagagtt atgatgatat gctggatgat ttcacttcat
     8941 cggacttaga agactgtcaa tcctgcatga tttaactaaa tatactcaat cagaagatta
     9001 agagaaatat attggctttt agaaaggctt tattgtaatg acaaaaaaac atcttaaaac
     9061 gcttgccttg gcacttacta cagtatcagt agtgacatac agccaggagg tatatggatt
     9121 agaaagagag gaatcggtca aacaagaaca aacccagtca gcttcagaag atgattggtt
     9181 cgaagaagat aatgagagga aaacaaatgt ttctaaagag aattctactg ttgatgaaac
     9241 agttagtgat ttattttctg atggaaatag taataactct agttctaaaa ccgagtcagt
     9301 ggtaagtgac cctaaacaag tccccaaagc aaaaccagag gttacacaag aagcaagcaa
     9361 ttctagtaat gatgctagca aagtagaagt accaaaacag gatacagctt caaaaaagga
     9421 aactctagaa acatcaactt gggaggcaaa agatttcgta actagagggg atactttagt
     9481 aggtttttca aaatctggaa ttaataagtt atctcaaaca tcacacttgg ttttaccaag
     9541 tcatgcagca gatggaactc aattgacaca agtagctagc tttgctttta ctccagataa
     9601 aaagacggcc attgcagaat atacaagtag gctaggagaa aatgggaaac cgagtcgttt
     9661 agatattgat cagaaggaaa ttattgatga gggagaaata tttaatgctt accagttgac
     9721 taagcttact attccaaatg gttataagtc tattggtcaa gatgcttttg tggacaataa
     9781 gaatattgct gaggttaacc ttcctgagag tctcgagact atttcagact atgcttttgc
     9841 tcacatgtct ttaaaacaag taaagttacc agataaccta aaggtcattg gagaattagc
     9901 tttttttgat aatcagattg gtggtaagct ttacttgcca cgtcacttga taaaattagc
     9961 agaacgcgct ttcaaatcta atcgtattca aacagttgaa tttttgggaa gtaagcttaa
    10021 ggttatagga gaagcaagtt ttcaagataa taatctgagg aatgttatgc ttccggatgg
    10081 acttgaaaaa atagaatcag aagcttttac aggaaatcca ggagatgaac attacaacaa
    10141 tcaggttgta ttgcgcacaa ggacaggcca aaatccacat caacttgcga ctgagaatac
    10201 ttacgtcaat ccggacaaat cattgtggcg tgcaacacct gatatggatt ataccaaatg
    10261 gttagaggaa gattttacct atcaaaaaaa tagtgttaca ggtttttcaa ataaaggctt
    10321 acaaaaggta agacgtaata aaaacttaga aattccaaaa caacacaatg gtattactat
    10381 tactgaaatt ggtgataacg cttttcgcaa tgttgatttt caaagtaaaa ctttacgtaa
    10441 atatgatttg gaagaaataa agctcccctc aactattcgg aaaataggtg cttttgcttt
    10501 tcaatctaat aacttgaaat cctttgaagc aagtgaagat ttagaagaga ttaaagaggg
    10561 agcctttatg aataatcgta ttggaactct agacttgaaa gacaaactta tcaaaatagg
    10621 tgatgctgct ttccatatta atcatattta tgccattgtt cttccagaat ctgtacaaga
    10681 aataggacgt tcagcttttc gacaaaatgg tgcgcttcac cttatgttta tcggaaataa
    10741 ggttaaaaca attggtgaaa tggctttttt atccaataaa ctggaaagtg taaatctctc
    10801 tgagcaaaaa caattaaaga caattgaggt ccaagctttt tcggataatg cccttagtga
    10861 agtagtctta ccgccaaatt tacagactat tcgtgaagag gctttcaaaa ggaatcattt
    10921 gaaagaagtg aagggttcat ctacattatc tcagattact tttaatgctt ttgatcaaaa
    10981 tgatggggac aaacgctttg gtaagaaagt ggttgttagg acacataata attctcatat
    11041 gttagcagat ggtgagcgtt ttatcattga tccaggtaag ctatcttcta caatggtaga
    11101 ccttgaaaag gttttaaaaa taatcgaagg tttagattac tctacattac gtcagactac
    11161 tcaaactcag tttagagaaa tgactactgc aggtaaagcg ttgttatcaa aatctaacct
    11221 ccgacaagga gaaaaacaaa aattccttca agaagcacaa tttttccttg gtcgcgttga
    11281 tttggataaa gccatagcta aagctgagaa ggctttagtg accaagaagg caacaaagaa
    11341 tggtcatttg cttgagagga gtattaacaa agcggtatta gcttataata atagtgctat
    11401 taaaaaagct aatgttaagc gcttggaaaa agagttagac ttgctgacag atttagtcga
    11461 gggaaaagga ccattagcgc aagctacaat ggtacaagga gtttatttat taaagacgcc
    11521 tttaccattg ccagaatatt atatcggatt gaacgtttat tttgacaagt ctggaaaatt
    11581 gatttatgca cttgatatga gtgatactat tggcgaggga caaaaagatg catatggtaa
    11641 tcctatatta aatgttgacg aggataatga aggttatcat accttggcag ttgccacttt
    11701 agctgattat gaaggtcttt atattaaaga tattttaaat agttcccttg ataagattaa
    11761 agcaatacgc cagattcctt tggcaaaata tcatagatta ggaattttcc aagctatccg
    11821 aaatgcagcg gcagaagcag accgattgct tcctaagaca cctaaggggt acctaaatga
    11881 agtcccaaat tatcgtaaaa aacaagtgga gaaaaattta aaaccagttg attataaaac
    11941 gccgattttt aataaggatt tacctaatga aaaggtagac ggtgatagag cggctaaagg
    12001 tcataatata aatgcggaga ctaataattc tgtagctgta acaccaataa ggtccgagca
    12061 gcaattacat aagtcacagt ctgatgtaaa tttacctcaa acaagttcta aaaataattt
    12121 tatatacgag attctaggat acgttagttt atgtttgctt ttcctagtaa ctgctgggaa
    12181 aaaaggaaaa cgagcaagaa aataaggaga aattaatatg attacacaag aaatgaaaga
    12241 gattattaat agtcagttag caatggtcgc gacggttgat gctaaaggtc aacccaatat
    12301 tggcccaaaa cgttctatgc gtctctggga tgataaaacg tttatttata atgaaaatac
    12361 agatggtcaa actcgtatta acattgaaga taatgggaaa attgaaattg cctttgttga
    12421 tcgtgaacgt cttttaggtt acaggtttgt gggaactgct gaaattcaaa cagaaggtac
    12481 ttattacgaa gcagcgaaaa aatgggcaga aggacgcatg ggggttccga aagcagttgg
    12541 tattattcat gtcgagcgta tttttaattt acaatctggt gctaatgcag gtaaagaaat
    12601 cacttaatat aaaaaagcct c
//
LOCUS       DATRME010000056        12508 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRME010000056 DATRME010000000
VERSION     DATRME010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12508
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(59..964)
                     /locus_tag="VBC23_001521"
     CDS             complement(59..964)
                     /locus_tag="VBC23_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6843459.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            complement(1180..1473)
                     /gene="rpmA"
                     /locus_tag="VBC23_001522"
     CDS             complement(1180..1473)
                     /gene="rpmA"
                     /locus_tag="VBC23_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6843460.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(1495..1833)
                     /locus_tag="VBC23_001523"
     CDS             complement(1495..1833)
                     /locus_tag="VBC23_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6843461.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            complement(1840..2154)
                     /gene="rplU"
                     /locus_tag="VBC23_001524"
     CDS             complement(1840..2154)
                     /gene="rplU"
                     /locus_tag="VBC23_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6843462.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(2189..2268)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(2344..3522)
                     /locus_tag="VBC23_001525"
     CDS             complement(2344..3522)
                     /locus_tag="VBC23_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6843463.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     gene            complement(3624..4838)
                     /gene="thiI"
                     /locus_tag="VBC23_001526"
     CDS             complement(3624..4838)
                     /gene="thiI"
                     /locus_tag="VBC23_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6843464.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            complement(4840..5985)
                     /locus_tag="VBC23_001527"
     CDS             complement(4840..5985)
                     /locus_tag="VBC23_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6843465.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            6237..6689
                     /locus_tag="VBC23_001528"
     CDS             6237..6689
                     /locus_tag="VBC23_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843466.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            complement(6732..8081)
                     /gene="gorA"
                     /locus_tag="VBC23_001529"
     CDS             complement(6732..8081)
                     /gene="gorA"
                     /locus_tag="VBC23_001529"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6843467.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(8205..9917)
                     /locus_tag="VBC23_001530"
     CDS             complement(8205..9917)
                     /locus_tag="VBC23_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6843468.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDI
                     EKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQSESLNIGDINLYNKIKQKIALI
                     DYCY"
     gene            complement(9925..10920)
                     /locus_tag="VBC23_001531"
     CDS             complement(9925..10920)
                     /locus_tag="VBC23_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6843469.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            complement(10974..11141)
                     /locus_tag="VBC23_001532"
     CDS             complement(10974..11141)
                     /locus_tag="VBC23_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843470.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            complement(11134..12462)
                     /locus_tag="VBC23_001533"
     CDS             complement(11134..12462)
                     /locus_tag="VBC23_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfB"
                     /protein_id="HEO6843471.1"
                     /translation="MIILFDFFDKKSKDLYYSLITSGLHGNAVVINDDGFLPQNINSP
                     YSFFCNMEGKNGNPLYFNQVPLPDLWEIKGNNIEAEIWDFSIKRAKIFYQEPKYKRQV
                     KNIDWFDNNKKVRYTDHYNRFGWCFARTHFDKNQNVTTKSYFDKDGKEVIVENFRTGV
                     IILNWLNKDYFFDNRVAFLNFYFSLMGWNLSRIWYNSLSTPFFVSYRMTYPGEDILFW
                     QEDIEDTIPANMRVLLESTNTRTQKVIVQKKNTYHKIKSMLPKEQQEKIGYLGFIYPN
                     KKNNKGRKDIFILTNSDQIEHLEVLVHHLSDYHFHIAAYTEMSFKLMSFSQEQNVTLY
                     PNISRTDLDNLFEICDIYFDINHGNEVDDVIRRAFEYNHLIFAFDNTCHNRELVLDSN
                     IISHTTCEQLINLMKNLSGSIMYLLEQQREQTSNETKERYKEILGGYGNA"
BASE COUNT         4026 a         2465 c         1769 g         4248 t
ORIGIN      
        1 tctaaagcaa tcaacaaaat agttattatt ggtataataa tctttctcat tacttctcct
       61 atgaattaaa tcccttaaaa tactcttcca taacagcaac aaattttaaa gccatctgag
      121 ataaattacg atcttttcgt ttgatataaa tcatttggtt atctagatga tcttctaatg
      181 gaataacagt gataccattc acactgcggc tatccaagaa tcctgagccc gtcgcataag
      241 cttgggttct ttctaaaata ccatttaatg ttgctctatc agtaacatta taggtaacag
      301 aactatcaag tacttccaca aaattctccg agtaataccg ataatcatct ctatcctgtg
      361 taaaacgtac ggttggtaat ccttctaaat ctgtcattat cagagatgtt ttactagcca
      421 agggatggtc tttcccaaga taaatatgag ttttaaaagg aataagctct acaaactcta
      481 accccaattt gtccaaccgc tgaagtagac cttttttatt ttgactattg atataaatga
      541 tacctatttc gctattccct tgagcaactt catctaagat acgtattgtt gttgattcga
      601 aaatacggaa atagctaaaa ttatcattac attttgaaaa cgctgtgatt aaaggaggta
      661 gaaaatcata atgttgactt gctattgaga attcttgctc tgtagcttca gattgcgaaa
      721 actgtttttc gaatgaatca aagcttttca caacttctaa agcattctca taaaaagtca
      781 tgccttgatt ggtcaacact gcaccagtag ttgtacgcgt aaaaatctga aaccctaatt
      841 ctgtttccaa atcacgaacg gcaacagata aacttggctg actaacaaat agttttgcag
      901 ctgcttcacg aaaagtacca ctatttgcga tagcaacaac ataacgcagt tgttgaatat
      961 tcattttatc acttcctcat tttagtaccc ttcattatac caaaaaataa ctatccttgc
     1021 ggaaaattct tcatgaatta ggactatttc actataaaag aacaacaaag tttatgagta
     1081 ttaatgataa atctcttaac atattgtctt ttgttacaaa atgaaagctc tgacaataat
     1141 aaaagacctt atgaatagtc ataaagtctt aactaggcat tattttgcga ttgggtaaac
     1201 agacacttgt tttttatcgc gtcctttacg ttcgaaacga acaacacctt caactttagc
     1261 aaaaagtgta tcgtccccac cacgtccaac gttagcacct ggatagatgt gtgtaccgcg
     1321 ttgacggtaa agaattgatc cacctgatac tgtttgtccg tcagctgctt tagctccaag
     1381 acgttttgct tgtgaatcac gtccgtttga tgtagaacct ccaccttttt tgtgggcgaa
     1441 aagttgcaag ttagcaagat tcatttttaa cataatgttg tgtcctctct taaattagtg
     1501 tgtcatgact actgtactaa caaattcaga agaatcttta gacagattag tcattccaag
     1561 aaggtaagac tcaaataata attgaacctt atcctcttta tgttgtggta tagaagataa
     1621 atctatcttc atataaccac cttcaacgtc attgataatc aattgcgctt ggcaggttgt
     1681 taaagcctct aatgaattaa taaaattaat tgataaagta ctaacagctg cacaaatcac
     1741 atcaaagccg tactcgccac taccagcatg accgctgatt tcagcactat aaagatagcc
     1801 tgattgatta cgagtaaaag ttgctttaat catgaaaaat taagcgttga ttgcgttgat
     1861 aacaactttt gtataaggtt gacggtgacc ttgtttacgg tggctacctt ttttaggttt
     1921 gtatttgtaa gaaacaactt tcttttgttt gccttgtttt tcaacagtac caacaacagt
     1981 agctccttca acaactggag taccaacttt agttgtttca ccaccaacaa gaacaacttc
     2041 gttaaatgta acttctgctc ctgcttcaac gtcaagtttt tcaacgtaga tagcttgtcc
     2101 tacttcaact ttaacttgtt taccaccagt tttgatgatt gcgtatgtgc tcatgatgca
     2161 cctcctataa aaatttcttg tgaggtttcc ctctgtgaag actcgcctat aaatcgtgag
     2221 agccttgccc tacttaatct aacgattttc gcgcggttgc acaggatgtg catttagtca
     2281 acattcaaat tttatcataa actctttttt tatgcaagat aaaacaaaat aaaattatca
     2341 actttaccac ttcaaattaa ctaactcatt catttcctta tatgctttat caactttatc
     2401 tttcatagtc tgatcaattg tagaacgttt cttaccacct tcaatataat cctctaaaac
     2461 tagcgtccta taatcataag ctggaaagcc ttcttcaaca taacggccat ttgcttttgc
     2521 ttctactagt gttggatgcg ccttaacttt tttaattact gttttttgac cttttttctc
     2581 tatggttaca tccattaata atcctctttc tgtccagata tcatcaaccg tttctaaacg
     2641 ctgatttgaa ataaaatttc ccatagagta aataataaat tttttctggc catctttttt
     2701 tatcacttca gacggttcaa caacgtgagg gtgtcctccg aatataatat ctgcccccca
     2761 tttaatcata ctatgataaa gtattacttg ttctgtagta ggtttttttt gatattctat
     2821 ccccatctgt ggcatgacaa tggtgatatc tgcttctttt tcagcctttt taatatcttg
     2881 cttaatcttt ttagtatcta aatcagacat atgtttttca tagtcactct ttgaaacatt
     2941 agcttccata ccattgtagc catacgaata acctaaaata gcaattttaa taccatttac
     3001 acgcttaatt aagatatctt ctttatttct atcctttaaa taaaccccaa tagtatccaa
     3061 tcccagacga ttaaaagtct tgacagtatt gatagcacct gccagttgtg aatccagaat
     3121 atgattatgt gctaagtcaa cgacatcata tccagtttct ttcatagtag ttgcaatctc
     3181 attaggagca ttaaataaag gatagcccgc taaaggatat tctgaactaa tcgtcccttc
     3241 ataatcacca atcgctaaat cagcgctctc aatccaagat tttacctctt taaaataagg
     3301 agtaaaatta taagtgccgt cgggttggcg cgcactggca tatagtacat cgtgcagcaa
     3361 aatatctccg tttgcaacca cacgtgctgt ttttacagta ttattatttg caaccttttg
     3421 ccctttagat tgtgctaaat ccttagataa ccccaaaata aaaatagcaa taataacggc
     3481 tacacaaaaa ataagtgttt tttgataagg gattcttgcc atacttgcct cctttattcg
     3541 tattatagca ttttttataa ccttatgaca gcacaatcat tttaataaaa aactacagag
     3601 taaatctgta gtttaaaaat tcattataat aaatcgtcaa tcaagtcatc aacatcatca
     3661 ctatctgctt gaggttgaat agtagttacc ataatccctg caactgccct ctctaccaga
     3721 ccttctacat ccatacgttt ctcatactgt tctgtattct taattttagg gttagttttt
     3781 gggcgatctg gtgcaaaaat tgtacagcaa tcctcaaatg gttgaattga aatatcaaaa
     3841 gtatctattt tttgagcaat atcaataatt tctagcttat ccatcgtgac cacaggacga
     3901 ataatcggtg ttgcagtgac agcattaata gcttgcatac tttctagcgt ttggcttgcc
     3961 acctgcccta aactttcacc gttaataata acaagaccat ttcggtcctc acgaatacga
     4021 tctgtaatac gcatcataaa cctacgtgtt aacgtcatca agtaggcttc aggagctttt
     4081 tccttaattt cctcttgaat ttctgtgaat ggaacttcaa taaattgaat attaccacca
     4141 aattttgtca atttacgtgt taaatcatgt gcttttttca atgcacctgg gctagtataa
     4201 ggaggacttg caaaatggac tgcttctata tctacaccac gttttaacgc taggtaacct
     4261 gctaccggag aatcaatccc accagacaac atcagcatcc ctttcccaga tgttcctacc
     4321 ggtaatcctc ctgcacccct aatatcctca tatgaaatat aagcagcctc atctcgtatc
     4381 tcgactttaa gattgatatc tggttgcttc atctgagcct taatatttgg caagactgaa
     4441 aaaacggcat ctcctaaagt atgatttaga gcacggctat ccaattcaaa tgagtggtca
     4501 ctacgttttg cggtaatttt aaaagtcatc ccatctttat aaacggaagt cataatttcc
     4561 tggacagctt ttaccaatgt atcaacattt ttttctactt taaaagatgg agaaaaagct
     4621 tggataccaa aaatctcttt taaggactct gcaacttcat gataatctgt accattgaga
     4681 tatacatgtc ctctatcacg atctgttttt actgaaacat ctggataaat ggagagtaca
     4741 tgctccatat tattttttaa cttattgatg aagcgcatac ggtttttctt cttagtagag
     4801 agttctccat aacgaatcat aatttctgaa tactgcatct tatcctctaa ctttcttagt
     4861 tttttcataa acttgtttaa aaatagttaa aaattgctct acttgtccca tatcattatc
     4921 atcatctaag ctgatacgta cagcagtttg ggctaactta gttgaaattc ccattgctat
     4981 aagagttcct gctggtttac ctgctttaga agagcaagcg ctagttgtcg agataaaaat
     5041 atcatgtccc tcaaaggcat ggacaagaac ttctcctcgg acacccttga taccaaacgt
     5101 gataatgtta ggtgaaaaac cttccttacc cgaaaataac gtaatatcct catatttggc
     5161 taattcatca tgaataatgg ttttcatttt acttatttta ggaatagcta ctacttcctt
     5221 gtccataacc attcgtaaag ctttagcagt agctgcaatc cctgcgacat tctcagtagt
     5281 tgaacgtaaa tcagattctt gtccaccacc tgttagtaat ggtgaaatac gttttccttc
     5341 tttaatatat agaaagccaa ccccacgaac gccgtgaaat ttatgactag aaaaagatgc
     5401 aaaatcaact cgctcagtca aataatcttt agtcggtatc ttacctattg cttgaacagc
     5461 atcaacatga aacgatatag taggtttatc tgacaacaag tctgaaatag cttttattgg
     5521 ctgaatagaa ccaatttcat tgtttattgc cataattgaa ataagaatcg tttctggttt
     5581 aattaatttg accagtgctt ctacgtctac aaatccttta tcatctactg gcgcataatc
     5641 aatctcaaac ccatactctc caagccattt agcagactct ttaacagctg ggtgttcgat
     5701 atcagaaaca ataatatgat ttccaaaatg ttgtttctca aacgcaagac ccttaataac
     5761 ccaattatcg gcctctgtac caccagaggt aaagaaaatt tcatttgctt taagctcaag
     5821 taaactagca atttgtttac ggctagcctc taaaatccga gaagatgttg ttcctagctc
     5881 atgtaaactt gaggggtttc catagatttt actagctact tcttgataag tccttaaaac
     5941 ttctggatag ggaatagtag ttgccgaatt atcaaaataa atcataaatc tgtccttcta
     6001 aagtcaatca gtgttattat agcataattt tcctactatt tatattagtt tgtgaacatc
     6061 ccaaaacaat atttatgata attctcataa tttatcacaa aactcactac ctattacact
     6121 aacatagctt agcttaataa cctaggttgc ttatttaaat gttttaaatt attttttctt
     6181 tagctttttt taggataaaa tcatataata gttgatgtac atttaaggag ggcgtcatgt
     6241 caaaaggata ttcaagacaa cagaaaaagt ctactaagac taatactgat tcaactcaat
     6301 cacccgctaa gcatataaaa acaggttttt ctgcacttca aaaaacaatt gcattagtcg
     6361 gtagtgtttt aagtattatc gttgcctcta ttactattac taatgcgatt aataatcaca
     6421 aagactctag taagactgat tcttctactt caaaaaccgt cattataaga gaaagtggac
     6481 aaaattctag taaccaagtt tctactgaaa aagatgagac aagggcatca tccagccaaa
     6541 cactagaaaa ttcctctgct agtagtactc cgagtagcag ttcaaattct gctgccaata
     6601 acgatcaaac atcatctagt actaacaatg atcaaacaaa taatactact accgatacct
     6661 caactattga aaatacaaca acaccttaaa aaagctaggt tccctagctt tttttgtatt
     6721 ctttgatagc gttagcgcat tgtaacaaat tcctcagatc cagttgggtg aatagcaaca
     6781 gtatcatcaa agtctgcttt agtagccccc attttgatag caactgaaaa accttgaatc
     6841 atttcatcaa taccataacc aacaccatga agcccaataa ctttttcctc ttttcctagg
     6901 gttatgagct tcatcttaac tgcttggcga ttactggtaa cagccgtata catagaggta
     6961 aaagttgatg tatagacttt gatattatct tttccaaatt gctcgatagc tgctgcttct
     7021 gaaagtccta ccgtcccaat tacagggtga gtaaaaataa ctgaaggtac attatggtaa
     7081 tctaattttt cgttatcttt atgattaaaa agtctttctg ataagcgacg acctgctgca
     7141 attgctaccg gtgtcaaggc aattttccca ttaacatctc caatagcata cacgccatct
     7201 acagaagtat tctcaaattc atctgttttg atataatctt tatcatttaa aacaatatcg
     7261 gtattttcaa gtcccatgtc tacatttggt ccacggccga tagcccatat tacacgatca
     7321 acgacaagcg ttttcccatt ttcagcttca aaaatcaact tgccaccttc atcgcgttta
     7381 agagatttag gtacatggtt agcatgtaaa gagatacctg atttctccat ttcagccata
     7441 acctcacttg ttaccatgtc atcaaatccg cgtagaatat ggtcttttct aaatgcaaga
     7501 tgggtttcaa cgcctaattc attaaccact ccagcaagtt ctgccgcgat atagcccgcc
     7561 ccaacaatca atatagaatt tggtaaggtc tcccatccaa aaaaatcatc agaagtctca
     7621 ccaagttcac ttccaataat atcagggtaa agagggtgtc cacctgttgc aatagtaatg
     7681 tgaggagctt tatattgctg accattcact tcaatagtat ggttatcaat aaaacgagca
     7741 aatccatcaa tcttttcgac cccattacgc tcaaaattag cggcatacga ctgtctagac
     7801 cgctgcacgt aagcatcgcg attagctttt agagtagtaa aatcaaaact aagattattg
     7861 gcttcaaaac cataacctga actatactta tggagtgtct cagaaacttg tgcaccatac
     7921 cacatgattt tcttaggtac acaacctaag ttaacacaag ttccacctac ttgtccacct
     7981 tcaattaaca ggacttttgc tccatacatg gctgccctat tagcggtacc actgcctgca
     8041 ctacctccac caataacgat ataatcatat tgttttgaca ttactctctc cttgaatcat
     8101 tgatacctat actctatcac aaagtgttta cagtcacaat tttctgctac gtaaacaatt
     8161 tatattaccg attatcatat cctaacttgc tgttaaaatt atttctagta acaataatct
     8221 atcaatgcaa tcttttgctt aattttatta tataaattaa tatcacctat attcaacgac
     8281 tcactttgat aatttaataa agtttcctta taatttttga tgtatttctc agaattaaac
     8341 cctaccattg ttaacagggc taacctctct tcctttctag atagcatctt ctctatatca
     8401 tgtcccgtga atctttttat ttcagtatga gagaaaactt gagaggttat gtataaacca
     8461 atatgagaaa atgtaatgtg ccttgatagc aaataaacat tatacagtgt gtattctcct
     8521 gttaaatctt ctaaaggaaa agtgatattg ttaaaaagtg attttctaaa gagcttcata
     8581 gaaaggagag gaaatatttg ttttaaatta tcgtggtaac tatgttcttg ctctaaccat
     8641 tcttgagcaa agaatgtttt ctcaaaataa ttatcatctg ttgcaaaata aacaaagcat
     8701 ttttcctctt cattaaaaaa agtataggtg gttgctgaga tatcagattt attagctctt
     8761 aatttattgt aaagataatt taagtaagac tcatcaagaa aatcatctgg attcacaaaa
     8821 gtaacatact tccctttaga tttctctaat gcgaagttcc atacagatgt aataacttga
     8881 tgttcttcat gaaataaacg aatacgctca tccttcatcg catatttctc gcaaatatta
     8941 ccacttccat ctgtagaacc atggttgact aagatcacct caaaagttga gtaaacttgc
     9001 ctaaggatag aatctaaaca ccttgataaa gaaccagaca tattatagac aggaagtatg
     9061 attgatatta attcagtatc cctgaattca gtatcatcga tagttattgt tttaacaatc
     9121 tcatcaatat aattctgact gtcaattaaa ttaatatttt catcatcgtt aaactcagcg
     9181 gtgtgtttat gagaaaattt tattttttga gtagctccca tcttatacca ttcatattca
     9241 gaaacaatat gaccgatatc aatggcttga aatcctctat cagataaatc tcttactaaa
     9301 atttttgcag tcggacctag catcaataag attatcctcc cttgagcata tctttcaaca
     9361 gcctcaagaa tttcatcata ttttatataa gcatttttag aaggacaaat aatccttttt
     9421 aaactattgc aattattaaa aagatcatta cctacacccg accgcgaagt ttccccttca
     9481 actaccaaaa tatcgcgttt gtcccaaagt aactttaatt ttttaaatga ttctacagca
     9541 gactccttat cttgtaagtc catatatggc cttgaaataa aagaagaacc ataccaatct
     9601 tgataacaat gttgcctata gaaattgcca aatttttgaa aatgattttt ccaaaaaagt
     9661 tgtgccgatt gattatatct ttgtcgattt tgaaacatat ctggaagaca tactaataga
     9721 tcattcgtac tttgcttatg aagaagttgc aataacctat ctgctaactc tgggttgtaa
     9781 tcttgataag gaatcgattt tcccattata atgtcaaatt caccgtcccc aaagcgaata
     9841 actgacgaat gattttccaa aatgtaatcc aaacttttgt ttatagggaa aactacgatt
     9901 tcttctagtt ctttcatatt cccctcacat gctatttaat gcactaaata ctgcatcggt
     9961 taatagtttt cgcgtaaaat atccttgtct aactaaagga ttaaaccgcc tgacatcgct
    10021 aacaatttcc atatattcct cttcagtagt actttcaact atattactaa tatcatcaat
    10081 tttttcaata ataaatccca aattattttt ttctattata tcttgattag caataccttt
    10141 ttgaataatc actggaattc ctgctgctat ataagcgccc agtttgtaag ggcaataaag
    10201 agattgatat tgtttatcac ggtcatccat ccagactaag ccatatcccc cttcgctcat
    10261 tttcataatt aattgttcgt ccgattgata tggctcttta accacagtgg tgggtaattg
    10321 catgttatgg tcagtataaa catgtaactc ggtaggaatt ctccaattct ttatgaagtt
    10381 aaaccgctct gggttcccag gaaaatgaac aagcttctta tgattaactg cttgtaaagt
    10441 aatattggtg ggatgatccc acattccttg aactaagatt ttttttactg tcaagccata
    10501 actttgaagc ttatcgacca ttgcttgact aggggctatt aaaacatctg cttcattata
    10561 ataagctata gttctatcca tcaaataaaa atttccatca aacattagcg gtacaacatc
    10621 atgtataaaa ataacaatct ttacaccaaa tatttttaat ttgtgaaata atttttcatc
    10681 aaaagttgta gtgttccatg taggtgtctg aaatatgaca atatcatttt ttctaagtcc
    10741 ggaacagatt ccatctaaac gcttactcat ttcctcagga gaatcagtat caatcggata
    10801 agaatgtatt cccagctcac gaaaacctaa gcttactgct atatctgtta ccatgttttg
    10861 agctatttgt gctgtactag tgattgaatg tccattcaag tttgtaatat atgtcctcac
    10921 acacttcccc tatctaaaaa acaacaggct tcttttctac cattaatagg aatctataaa
    10981 aaatatttaa ataacgacaa tataactgct acagtaacaa gtataagtac taccaaataa
    11041 aaagtaggtc gcttgtcatg ttcactctct ggttctttat atctaagttt atcaatactc
    11101 attttaattt ctttattgag taattcattt cttttaggca tttccatacc ctcctaatat
    11161 ttctttataa cgctcttttg tttcattact tgtttgttct ctttgttgct ctagcaaata
    11221 cataatggag cctgataaat ttttcattaa atttatcaat tgttcacagg ttgtgtgaga
    11281 aatgatattg ctatctaata ctaactctct gttatgacag gtattatcaa aagcaaagat
    11341 aaggtgatta tattcaaatg cccttctaat aacatcatca acttcatttc catgattaat
    11401 gtcaaaataa atatcacaga tttcaaataa gttgtctaaa tcagttcttg aaatattcgg
    11461 atatagcgtt acattttgtt cttggctaaa tgacatcaac ttaaaagaca tttctgtgta
    11521 cgctgcaata tgaaaatgat aatcagacaa gtgatgaacc aacacttcta aatgttcaat
    11581 ttggtcagag tttgtcaaaa taaaaatatc tttcctaccc ttattgttct ttttattagg
    11641 atatataaag cctaaataac caattttttc ttgttgttct ttaggtaaca ttgactttat
    11701 tttatgatat gtatttttct tttgaacaat tactttctga gttctagtat tcgtactttc
    11761 taataacacg cgcatattag caggaatagt atcctctata tcttcttgcc aaaataaaat
    11821 atcttctcct ggataagtca tccggtaaga aacgaaaaat ggagttgata aggagttgta
    11881 ccatattcga gacaagttcc aacccataag gctaaaataa aaatttaaga atgcaactcg
    11941 attatcaaaa aaataatctt tatttagcca attaaggatt ataactcctg ttctaaaatt
    12001 ttcaacaata acttctttac catccttatc gaaataagat tttgtagtca cattttgatt
    12061 cttatcaaaa tgtgtacgtg caaaacacca tccgaagcga ttataatgat ctgtgtatct
    12121 cactttttta ttattgtcaa accaatcaat attttttact tgacgtttat acttcggctc
    12181 ttgataaaaa attttagctc ttttaatact aaaatcccaa atttcagcct ctatattatt
    12241 tccctttatc tcccaaaggt cagggagtgg gacttggtta aaataaagag gattcccatt
    12301 tttcccttcc atattacaaa aaaatgaata aggagaattt atattttgtg gaagaaaacc
    12361 atcgtcatta atgaccacag catttccatg taaacctgaa gtaattaaag aataatatag
    12421 atctttgctt ttcttatcaa agaaatcaaa tagtataatc atttaataac ctcctcaaaa
    12481 aaaaaaactc caattttcta taatttta
//
LOCUS       DATRME010000057        12311 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRME010000057 DATRME010000000
VERSION     DATRME010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..483)
                     /locus_tag="VBC23_001534"
     CDS             complement(<1..483)
                     /locus_tag="VBC23_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6843472.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
     gene            complement(635..5936)
                     /locus_tag="VBC23_001535"
                     /pseudo
     CDS             complement(635..5936)
                     /locus_tag="VBC23_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(6179..8170)
                     /gene="uvrB"
                     /locus_tag="VBC23_001536"
     CDS             complement(6179..8170)
                     /gene="uvrB"
                     /locus_tag="VBC23_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6843473.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(8231..9151)
                     /locus_tag="VBC23_001537"
     CDS             complement(8231..9151)
                     /locus_tag="VBC23_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6843474.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGDIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            9334..11517
                     /locus_tag="VBC23_001538"
     CDS             9334..11517
                     /locus_tag="VBC23_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6843475.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            11517..12257
                     /locus_tag="VBC23_001539"
     CDS             11517..12257
                     /locus_tag="VBC23_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6843476.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
BASE COUNT         3822 a         2355 c         1991 g         4143 t
ORIGIN      
        1 tcctagctct tttttgttta ataaaatttt tcttataaaa ccatcttcaa gtcgcttact
       61 ataaaacatc tctgagatgt catcttcgtc aaatactctc aaaaggttaa gcatatcata
      121 tgctagttca atcttatcaa gttgtgaacg ttcaatccat ataacatcac cttcatctga
      181 cgattgtaat tcacctgaaa aatcatatgt tttataaagg aataccaaat aacggatgcc
      241 ttcatctgtg taccaatgct tcataccaac taatttggga ttacttattg ttaaacctgt
      301 ctcctcgcga acttctcgaa cgagagcatc atacaagctt tctccttctt caatatggcc
      361 gcctggtaga gcagctcctg accaagaata acgtttagga tcacggattt gcatcacaat
      421 acgtccttta ttatcttcaa taagacacat attagttaaa ataacttttt gactgcgtga
      481 cattatttcc tctagtatat aaatttagtg atagagttac aataccattt atttatcaat
      541 ttttctacaa aataatagat taagttaaaa ctcccttaat aatttatcca aaggaatttt
      601 agcttaataa attagaaaac taatttaatc aaaatcaata tttctttgaa gcttttttaa
      661 ggcctagtaa accaagtaca attactatga gtgcaattcc tttagccagt aaattattat
      721 tgtcatcact tgtccttggc aattcatgtt ttgccgctct agacattgga atcttagttg
      781 ttttcttcgc ttccatttga ctatgaacaa atgggggtgt ctttgtactc cctttcatgc
      841 ctacagctat cacttgtggt ttatccattt gatgtttaga tatttcttta aattcaccat
      901 gaactgctgg atcaacctta tgccctttta acgactctgt ttcttttttt agttttgtgt
      961 cgtcagagct tttatgatca tttttcaaac gtctgtcaga gttgatttct tccattgttt
     1021 ttacaatttg tatacttgtt tcgaaatggt tatatgcacc atttttaaat atcaaaccct
     1081 ttatagtatg atttcccact ttattatcga ctatttctgt caataaaata gagctctcct
     1141 tttaacagat ccaaaaggtc cgtaattcct tttggatctg ttaaaaggag agtttgtttt
     1201 ttcaaatgtg gttttagttt tgaatctact ttaaggtttg aagttgataa taatgcttca
     1261 aactctctaa ccacagaacg accaaggggg tcagatattt ttgccgtaaa gctcactttt
     1321 ttgtccatga gttggactgg cttaatcaat tccgcagtct gattgtgggc tagggataat
     1381 aaattatcga tagaggtaag atcttgcatt tggaaagagg ttttgcgtaa acttgctgat
     1441 aattcaggga agttgccgat tgttataatt ttttcagtga ctttacctga tttatcaact
     1501 gctgatactt tgatgacgta acgcccttcg aaacctttta attggtaaga gtcaatactt
     1561 tgtccttctg tgtagggctt gccattaaca gttatcgtta aatttttaag gtgattatct
     1621 tctgctttag ctataaccac tttgactttt ccggataata aatctgcttc attaatagtt
     1681 aacttcggtg tatccacatc attaactacc ttataatgtt cagaaacggt attacctgat
     1741 gtatctaccg ctgaaatcgt aacaatatct cctgctgctg cttttatctt atgagtaaat
     1801 gctcatctat tactgataaa ttcaccactt gaatatttaa catcgacgct atcaccatta
     1861 atagaaagac cccaaccttt tacattatca gaaacgtgcc ctttgatttc aatttctcca
     1921 ttttgagcat agatttgacc agtgataaag ttatctttgt cagcagcttc ttctatgtta
     1981 gtcttgtcca gttctaattt tggaggttgt acatcaacgg taatatgata gccacgacta
     2041 aataccaatt tatcattctt attataaacc ttcaaattca tgatgttatt cccagacata
     2101 aatttgaccc atgtggtttt aaatgtgtca gagtcatcac catacatata aatagggttg
     2161 gatactgtac gatatgggtc ggaatgtttt ggatctttag cgttctcatg aaggttaaca
     2221 actcttattt ctgcacgata gccatctttt aataagagag aaacactatg ctccaagctt
     2281 tcattttcaa catttcgaat tttatttaca gttcgaattg aatataccgt ttctcgagtt
     2341 aataataaac catcctcgat atcagaaata ttttcttcaa aatcaaaatc tttttcttcg
     2401 ttaaggtcat catcgttatc atcccagata tcgtcatcat ctgaactggt tgcttgagct
     2461 ttcatattgc gatgagacag tgactgtggt tgtaatgtat tatcttcctc actagagtaa
     2521 gatttctctg atacttcttc tgattctgat aaagcaattt ccttgtttga attcccagca
     2581 tagtctgcaa cttttatatc cagatcagat tctcttaacg ttaactcatc aatatgatag
     2641 tgattttcgg caattttttt aactgtgata ggcttttgtg tctctttatt acgaacaatt
     2701 acttctttaa cacgatgatt atcatgggtt aaaatatcaa agccgttatt aactttttta
     2761 agagaaaaag ttggtctttc attatctatt aaaattggaa ggtattggac ttgataagga
     2821 tctgtaggat ttgaacgtgc acgtaactta aagtaatata agcctggctt tgcagcttta
     2881 aaatcaccag tcttaggatc ataaaacttt ccatcccaaa ctagcgatga ttctgtcgtt
     2941 ctcaaagcat cataatatga gtcttcttcc tcatttcttg atttgtaata tttctccacg
     3001 aaatgtttca ctgttatacg acgaagtatt ggtgaatcgt cttttgcttc tgatacaata
     3061 gagaggtcaa aatctgatgc tgagcgtaat aaagaaaatc gaggtgctac acgcacatta
     3121 gacatagtaa atagggatgg attgaccttt ttactaccta tttcttgtcc gatttcataa
     3181 aatttgttag ttccgtgtcc aaccaaagtt gcttgcatca aggtcgttaa tttattttta
     3241 gaacgactat cccacatagg tgcgtcaaaa attggttctt aatcccatgt tcctgagaag
     3301 cccatgtaag gagctgagat atcttgtcca tctttatcta aagttttgaa atacaaatac
     3361 ccttcagcaa aatcttgtat aggttttttt atctcaagat ctatatcaag ctctatggct
     3421 gattgcggtg ctagtgagat aactttaccc atttgtaaag ttgctccctc tattacttta
     3481 gcgtgaactg cctttaaaac gacattttca tacccctgat tagtcacttt atcaacaatt
     3541 ttagttgtta aaatcgctcc tggttcaata ctaaagttac gacgtttttg tcctgtattg
     3601 accaatttca aggtaaatct tttatggttc ttaatctctt ttatagagaa tgatcctgtt
     3661 ttatcaccaa aaacaagaac atcgttagct aaagcttttt ctaagttaac catacctgca
     3721 ccttgacgtc gtggtgaaat ttctaaagct ttatttagag tatctttcaa aggttgactt
     3781 gtattcatca agacaatctt tgtcaaatca acgtttgtca aaccagctaa cattggaagt
     3841 tttaatgcca ttatttcagc tactttactt tttactaatg cactaatggc agctacttga
     3901 ggggtagcca ttgaagttcc agacatgtac ccataggtac cgtcgttaaa agtagaataa
     3961 atatcttctc ctggagcaac tacatctggc ttcaaatgca aatctggtgt cactccccaa
     4021 ctagaaaagc ctgaaattgt aggatttgga cggcgaacaa aggtttcttt aggtaaaaat
     4081 tctaatttag cagttttgtt tttcttgatg tagtcaatca actgatcacc atcttttttt
     4141 gatagactaa tagcccattt accttctggt atatcatagt gataatcaaa catggggtga
     4201 gtatcaaaat tcccagaact ataataagca ggttcattta tcataataac ccctttagca
     4261 attccgataa aacgttttag cttatcttta acgccctctc ctccacgctc aacgacgata
     4321 acttttccag tataactttt aaggtactct tctgagatat cttttttcaa ctggccttta
     4381 ccaacataga ctagatcaaa tgttttattt ttgagcattt ttaatattgt ttgactttta
     4441 tgtcccagtt ctgcatatcc taatttaaga tttccaactt tcaaaacatc taaaagaaga
     4501 gcactatttt gaactgaacc aactccaata accttatcaa cagctgcaac agacaccata
     4561 gcagcagtat cttttgttcc caaagagttg tcaacgacat tatcgtaggt agtagatgaa
     4621 gccgaattac catagtttcc cattgctgct actacgataa cacccttgtc agtcaccttc
     4681 ttaattgtct cataccaaag ttcacctgga cgtcctgttc ccaaatatcc aaagcttaaa
     4741 ctaataacgt ctgcccctct tttagcggca tcttccattg catggaagac tgcatcagag
     4801 ccggtccact ctgcagatgg atcttctcta tttgttgaaa ttttgtacac taataactgt
     4861 gcattagggg caactccttt aaaaccatcc tttgcatttc ccgctaagat acctgctaaa
     4921 tgcataccat gagggcgctg aacgtcgtct ttcaattcta aattatcgcc tcgttgataa
     4981 ttgtatgcaa aaggaacttt atttgtataa ggagccttaa tatctttaat acgacgcttt
     5041 gccatcacat ctggatcatt ctcatccaat cgcatggcag gatgatggat atctacacca
     5101 ctatcaatag tcgcaataag tagccctcta ccgtcaactt tactattttg tttagctttt
     5161 aaacgtgcag ctaagacatg tgtccattgg ttagcagtaa acatttgagg ttttaatact
     5221 tgaactgctt caatatgttt tatctcttta atcttaccaa ttttttcaat ttctttttca
     5281 tctactgcta atgaatagcc attaaaaagg ctactataag ttaacttaat acgagtgccc
     5341 ggaattgctt taagggcttc atctactttt tccttattta cttgatcaaa tgttacaata
     5401 acgcgcttat acactttaga atcagatggg ggagttggct tcgtcgaagc attattcacg
     5461 acatttttca tatccacttc attagcctta ttaggcttat tagtgttttt gttttcctca
     5521 cttgaaggtt gtgatgaagt atttaataac ttttcacttg attgtacttg ctttttagcc
     5581 tgtaaagagg tttcatcttt ttgatttttc gtttgttctt gaacgacttg tttaacacca
     5641 gcaactttat gtgcaggttg ttcactcttt agtatatttg aatctacaga tgatttattc
     5701 tcaagctttt gtgagttact cgaggctatt gttggagggg ttactgcatt actttcagta
     5761 cgtgactgtt taacttcgga aggcttgata gttgtgtttt tttctaaaag ctgactagta
     5821 ttcgcactag tatgagggac tactgtgtct gcatctacca ctacagaggt cgctaccgca
     5881 tttacagcta aaaacgcaat cagtacaccg gttgttttta aagaaattct tttcataatt
     5941 ccactcattt ttaagttaga ttattttatt tgttaagcaa gcttaattta agcacttttg
     6001 tatagagttg tcaatatcta tttgctaatt aatgagtatt ttttaagaat ttttatctta
     6061 tttagattag ttctttttaa ccaaactaaa aagctaactc tacctgctag agtcagctta
     6121 tttaataatc tataaccttc tgctatctcc taaagagaga accagatgag agataagttt
     6181 aatcgattgc ttttagttct aagataacat ctcggatttg tgcagctagc tcaaagtcca
     6241 ataattcagc tgcatcttgc atttgctttt gaagagcttt aattttagct tgcctttctt
     6301 ttttagataa actactgtaa tcgacaactt tttcaggctt atcgctatca ttacttttag
     6361 taatagcaat taaatcacgg atttcttttt ttattgtttg tggcacaatt ccatgctttt
     6421 cattataatc catttgtaaa cgacgacggc gagcggtctc atccatagct ctttgcatag
     6481 aatctgttat tttatcagca tacataataa catgaccatt actgttacgt gctgcacgtc
     6541 cgattgtttg gataagacca cgctcattac gaaggaaacc ttccttatca gcatctaaga
     6601 ttgcaacaag ggaaacttca ggaacatcta ttccttcacg taagagatta ataccaatta
     6661 aaacatcaaa cacaccaagt cgtagatcac gaataatctc tgtacgttct aaggttttaa
     6721 tatcgctgtg catatactta actttaactc ccatttcttt gagataatct gttaagtctt
     6781 ctgccatacg ctttgtcaat gttgtaataa aggtacgctc acctttttca gtcctcaaat
     6841 tgatttcacc aagcaagtca tccatctgcc ccatacttgg tctaacttca acttctggat
     6901 caagaagtcc tgttggccgg ataatttgct caacaactgt atctgtttgc tccatttcat
     6961 agtctcctgg cgttgcagat acatagacaa tttgatggac atggctttcg aattcttcac
     7021 gacgaagtgg acggttatct aaggcagatg gtaaacgaaa accgtaatta accaacattt
     7081 ctttacgcga acggtctcca ttatacatcc ctttgatttg ccccattgtc atgtgactct
     7141 catcaatcat aatcaaaaaa tcttctggga agaaatctaa caaagtaaaa ggtggctcac
     7201 cttcacttct accatccatg tgacgtgagt agttctcaac accatttgta tatcccattt
     7261 cacgaagcat ttcaatatca tattctgtcc tttgtcgaat acgttgcgct tcaatcaatt
     7321 tcccttcttt ttcaaaaagt tctacttgat tttccatttc agcttgtatt tttgaaatag
     7381 cttcttccat atgttcatca tttgtcataa agtgtgttgc tgggaaaata gctaaatgtt
     7441 ccacttctcc tagcacacgc cctgttaacg attctatttc tcttatacga tcaatttcat
     7501 caccaaaaaa ttcaattcga aaggcatgct catcacgact cgcaggaaaa aattctacaa
     7561 catcgccacg aacgcgaaat tttccacgtt gaaaatcaat atcgttacgc tcaaattgaa
     7621 tatcaacgag attgttcaat aactgatcac gtgaaatttc ttgacctggc ctaaggctaa
     7681 caacactgtc tgcatactct ttaggagaac ctaaaccgta aatacaagaa acagatgcta
     7741 caacaataac gtcatttctt tctaataacg aagaagtcgc tgagtgacgt aatttgtcaa
     7801 tttcgtcatt gactgagcta tctttttcaa tataagtatc cgaagaaggc acataagctt
     7861 ctggttgata ataatcataa tatgaaacaa agtactctac ggcattgtca ggaaaaaatt
     7921 ctttaaactc tccataaagt tgacctgcta gggttttatt atgtgcaatc accaaggttg
     7981 gcttgttaac ttgggcgata acttggctca ttgtgtaggt tttaccagta ccagtagcac
     8041 cttttaaaat ctgagctttt tctcctcctt caatattgtc aaccaacgtt tcaatagctt
     8101 gaggttgatc tcctgaagga ctatatttgg aaactaattt aaatcggtta gtatcttttc
     8161 tatctatcat ttctttacct cgattacttt attttaacat agaaaaaaga aaaagactga
     8221 gctgcaacac tcaatctgtt tcttcagtct taattagcca tagaaggtat ataattgcag
     8281 ctaagagaac aaaaatagaa atcaaacttc cttctattcc gaatgcacca cctgatatcc
     8341 agtccggtgc gttttcttta acggtaaaat gcaaaagaga tgctcctgct ttctgaccac
     8401 ttactgcaat accatagaga ttaccttggc taaaattcca agctccatgt aaggctgcaa
     8461 cgctccaaat atcaccactt ttaatcatat acaaggacat taaaactccc gaacatataa
     8521 tacttacaat agaaagaaaa gtgacatggg catttaccaa gtgcaaaatg ccaaataaag
     8581 tggatgagac ccctatggca acagctaaat gaaaacgatg attaattaga ggaagaagcc
     8641 accctcgtgt tagcaattct tccgtaccgc tttgaataag ccaaaaagga aggagagtta
     8701 caacatagaa aatcgttgcc ttagaaacat catagctatg aaagtcaagt cctcccaata
     8761 tataagcagt gatgacagaa attgaaaata gaataaaacc tatactccag ccttttataa
     8821 gtgaccacag agttctttta aactttgtag aaaaacccaa ttctgaaaaa ggtctttttt
     8881 ctacgaattt tacccagata agcaaaagga atgctgaaaa actaaaggtt cctaattctc
     8941 catagatatt attaaataaa ttaataaact caaaaaaagt ataggaagaa aacttataga
     9001 gtacagaaga cactagaaca attcccacac cacctagagt tagaccgacg aaaaaaaata
     9061 ggtataccat aaaacaagca aataacaata atagccatga cggccagttt ttatagacag
     9121 cgaactgact atttaaaatc cttttattca taataatcct aaatcctttt tgtgtgatga
     9181 cactagaaca attatatcat aatactaact aaagatatat ataaaattaa cataacatac
     9241 atgttacgtt ttctaacata gaaatgacat tttgttgctc tatcttattt attttgatat
     9301 aataaaacac atatttataa aaaggagatt tacatgaagc acaagttaaa agcttttacg
     9361 cttgctttac tctcaatatt ctttgtgttt ggtggaaagg tcagtgcaga gactgtgaat
     9421 attgtttctg atacagcata cgctccattc gaatttaaag attctgatca aacttataaa
     9481 ggaatcgatg ttgacatcgt taacgaagtc gctaagcgtg ctggctggaa tgttaacatg
     9541 acgtatccag gttttgatgc cgcagttaac gctgttcaat ctggacaggc agatgcgcta
     9601 atggccggaa ctactgttac tgaagcacgt aaaaaagtct ttaatttctc agatacttat
     9661 tacgatactt ccgttattct ttatactaaa aataataata aagtcacaaa ctacaaacaa
     9721 ctaaaaggaa aagtagtcgg tgtaaaaaat ggaacagctg ctcaaagctt cttagaagaa
     9781 aataaatcta aatacggcta taaagttaaa acatttgata caagcgacct aatgaataac
     9841 agccttgatt ctggttctat ttacgccgct atggacgatc aaccagttgt gcaatttgcg
     9901 ataaatcaag gaaaagctta cgccattaac atggaaggcg aagcagttgg tagctttgca
     9961 tttgctgtca aaaaaggtag tggacacgat aatctaatta aagaatttaa cacagctttt
    10021 gcacaaatga aatcagatgg cacttataat gacatcatgg ataaatggct tggaaaagac
    10081 gctacaaaaa caagcggcaa agcaacaggt aatgccaatg aaaaagcaac tcctgtaaag
    10141 ccaagttata aaattgtttc tgattcttca ttcgcaccat tcgaatatca aaacggtaaa
    10201 gggaaatata ctggttttga tatggaatta atcacgaaaa ttgctaaaca gcaaggtttt
    10261 aaacttgata tctcaaatcc aggttttgat gccgctttaa atgctgtcca atctgggcaa
    10321 gctgacggtg ttattgcagg agccacaatc acagaagcac gccaaaaaat ctttgatttt
    10381 tctgatcctt attacacatc tagcgttatc ttagcggtta aaaaaggaag caatgtcaaa
    10441 tcataccaag atttaaaagg aaaaacagtt ggtgctaaaa atggtactgc ctcatatact
    10501 tggttatcag accacgcaga taagtacaac tatcatgtta aagcatttga tgaagcatct
    10561 acaatgtatg atagtatgaa ctcaggttca attgatgctc taatggatga cgaagccgtt
    10621 cttgcttacg ctattaatca aggtcgtaaa tttgaaacac ctatcaaagg tgaaaaatca
    10681 ggcgatatcg gatttgcagt gaaaaaaggg gcaaatccag aattaattaa aatgtttaac
    10741 aacggtcttg cttcactcaa aaaatcgggt gagtacgata aacttgttaa aaaatacctt
    10801 tccacagcca gcacttcttc aaacgataaa gctgctaaac ctgtagatga atcaactatt
    10861 ttagggttaa tttctaataa ctacaaacaa ttgctatctg gtattggaac tactttaagt
    10921 ttaactctta tctcgtttgc gattgctatg gttattggta ttatctttgg tatgatgagc
    10981 gtatcaccaa gtaatactct ccgcacaatt tcaatgattt ttgttgatat tgtccgtggt
    11041 attccactca tgattgtggc cgcttttatt ttctggggta ttcctaattt aatcgaaagc
    11101 atcacaggtc accaaagtcc aattaatgac ttcgttgctg ctactatcgc tctttcttta
    11161 aatggtggtg cgtacattgc tgaaattgta cgtggtggta ttgaagctgt tccttctggt
    11221 caaatggaag caagtcgcag cttaggtatt tcttacggca aaactatgca aaaggttatc
    11281 ttacctcaag cagtacgcct tatgttacca aactttatca accaatttgt catctcatta
    11341 aaggatacaa caattgtatc agcaatcgga cttgtggaac tcttccaaac tggtaaaatc
    11401 ataattgcac gtaactatca atcattccgt atgtatgcta tccttgcaat tatgtatctt
    11461 gtaatcatta cccttctcac ccgtctagca aaacgtttag aaaagaggct taaataatgg
    11521 cagaattaaa aattgatgtc caagatttac acaagtcgta tgggcaaaat gaagttttaa
    11581 aaggtattga tgctaaattt tatgaaggtg atgttgtttg tattattgga ccttctggtt
    11641 caggaaaatc aactttcctt cgcacattga acttacttga atcaatcaca agtggaaaag
    11701 ttgttgtgga cggctttgag ttatcaaatc caaaaacaga catagacaaa gcacgtgaaa
    11761 atatcggaat ggtatttcaa cacttcaatc tttttccaca tatgtcagtt cttgaaaaca
    11821 tcacttttgc tcctatagaa cttggtaaag aatctaaaga agcagctgaa aagcatggga
    11881 tggaattact tgaaaaagtt ggtttagccg ataaagctaa tgccaaacca gatagtttat
    11941 ctggaggaca aaaacaacgt gtagctatcg caagaagctt agcgatgaat ccagatatct
    12001 tactttttga cgaaccaact tctgcacttg atccagaaat ggttggagat gttctcaatg
    12061 ttatgaaaga tttagctgaa caaggtatga ctatgttaat tgtgacacac gaaatgggat
    12121 ttgctcgtca agttgccaat cgtgttatct ttacagatgg tggtcgtttc ctcgaagatg
    12181 ggacacctga gcaaatcttt gatacacctc aacacccacg tctccaagac ttccttaata
    12241 aagttttaaa cgtttaataa aaaaagatga cttcgtgtca tcttttttta tatcctgatc
    12301 acaaacaaat t
//
LOCUS       DATRME010000058        11400 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRME010000058 DATRME010000000
VERSION     DATRME010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            63..860
                     /locus_tag="VBC23_001540"
     CDS             63..860
                     /locus_tag="VBC23_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6843477.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            1325..2287
                     /locus_tag="VBC23_001541"
     CDS             1325..2287
                     /locus_tag="VBC23_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6843478.1"
                     /translation="MKKITKMVISSVLASVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VLFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGCQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            2303..3199
                     /locus_tag="VBC23_001542"
     CDS             2303..3199
                     /locus_tag="VBC23_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843479.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKASKTLTKEG"
     gene            3204..4007
                     /locus_tag="VBC23_001543"
     CDS             3204..4007
                     /locus_tag="VBC23_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6843480.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            4182..4466
                     /gene="groES"
                     /locus_tag="VBC23_001544"
     CDS             4182..4466
                     /gene="groES"
                     /locus_tag="VBC23_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6843481.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            4562..6184
                     /gene="groL"
                     /locus_tag="VBC23_001545"
     CDS             4562..6184
                     /gene="groL"
                     /locus_tag="VBC23_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6843482.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(6216..6580)
                     /locus_tag="VBC23_001546"
                     /pseudo
     CDS             complement(6216..6580)
                     /locus_tag="VBC23_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6593..7330)
                     /locus_tag="VBC23_001547"
     CDS             complement(6593..7330)
                     /locus_tag="VBC23_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6843483.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            7489..8268
                     /gene="udp"
                     /locus_tag="VBC23_001548"
     CDS             7489..8268
                     /gene="udp"
                     /locus_tag="VBC23_001548"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6843484.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            8289..9491
                     /locus_tag="VBC23_001549"
     CDS             8289..9491
                     /locus_tag="VBC23_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6843485.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            9521..10192
                     /gene="deoC"
                     /locus_tag="VBC23_001550"
     CDS             9521..10192
                     /gene="deoC"
                     /locus_tag="VBC23_001550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6843486.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            10259..>11400
                     /locus_tag="VBC23_001551"
     CDS             10259..>11400
                     /locus_tag="VBC23_001551"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6843487.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMCDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISA"
BASE COUNT         3627 a         1807 c         2414 g         3552 t
ORIGIN      
        1 cgaaacaaaa atttaagtgt tagactaaag taagcgcgta caattatatt ttggaggata
       61 atatggtaag gctaatattt agtgatattg atggtacttt aattaacagt aattttaaag
      121 taacacctaa gacacggcag ggaattaaac aaatagtagc tcaaggggct actttcgttc
      181 ccatatcagc taggatgcct gaggcaatta ctccgattat ggaacaaatt ggaatagatt
      241 cttatattat ttcctataat ggggctttaa tacaagatat gcaacaaaaa acaattgctt
      301 cccacacaat ggatggtcaa gttgccttac aagtttgttc ttatgtaagc aagcattatt
      361 cgaaaattgc atggaatgtc tatcgttatc atgaatggta ttcctgtgat aaagaaaatg
      421 agtgggtcca aaaagaggag gagattgttg gtttacaatc taaagagatg agtttgatgg
      481 agttagagaa gcaagatagg attcataaac ttctcttgat gggagaacca tctctgatgg
      541 gagaattgga gaacactttg aaagctcaat atccacattt atcaatcgca caatctgctc
      601 cttactttat tgaaataatg gctccaggta ttgaaaaagg aaaatctgct aaaactttag
      661 cagactattt agatatttct cttgcagact ctattgcgtt tggtgataac tataatgatt
      721 taaatctgct tgaaattgtg ggtaaaggtt ttgtcatggg gaatgctcct aaagatttac
      781 aagagagaat tggaaatgta actcaggata atgataacga tggtatttat tatgctttag
      841 tagagtatat ggaaaattaa ggatagcaaa tctaaatttc cttaaaagtg tgttatatca
      901 acgaatgact attatttaaa ttatctatct aacaagaatt ttcaaaaaaa tcaaatttaa
      961 ctattgacaa ttatttttaa atgacgtaaa cttataggca accataaaca aggagagaat
     1021 aatatgttaa tcaaatcgtc tattattaaa caaacaaata cttattatta ctttagtttc
     1081 tttagataga cgtttgtagc ctcaaggata caaacgtcac gcaaagtttg tatctatgaa
     1141 gctaaagcat tttatgatga aagcagagcc gattagatca tttcaataat ctagttggtt
     1201 aaggcaatat tttttcgccc tcccgtgcac agaggaattg agagaaataa ttaaccaact
     1261 agatgtaggg tctagttggt tttttgtttt atggaaaatt tgatggtatt acaaggagat
     1321 agtaatgaaa aaaataacaa agatggtaat tagcagcgtt ttagcatctg ttctgatgac
     1381 agcttgttca caatcttctc aaaatggtca atctgatgta aaacatattg gtattttaca
     1441 atatgtagaa catccctctc taactgccac taggaaagga tttataaaag aattagctaa
     1501 agaaggctat aaagatggca aaaatatcaa aattgagtat aagaatgctc aaggggatca
     1561 gtcaaatatc caatcaattt ctgaaaaact cattaaagat aataaattag tattgggaat
     1621 tgcgactccg gcggctcaat cgttgacaac ggtgtcaaca gaaacaccta ttttatttac
     1681 agcagtgaca gatccggtat cagccgaatt agtaaaatca atgaaaaaac ctgaaggttt
     1741 agcgactggt acaagtgaca tgtctccaat taaaaagcaa gtatcccttt tgagaaaagt
     1801 aatgcctaag gttaaacgtg ttggtattat gtataccact agcgagcgta actcagaagt
     1861 tcaggtaaaa caagcaaaaa aaatcttcca agaggcgggg attaaaacaa gtgttaaagg
     1921 tatttcttcc actaatgatg tacaagatac tgctaagagt ttaatgtcta aaacagaggt
     1981 tctttttgta ccaacagata atattatagc cagctcagta acgctgttag ggaatctatc
     2041 gaaagaatta aaagtgcctg ttgtgggagg ttcagcagat atggtaccaa gtggtttgct
     2101 cttttcttat ggggctgatt atgaggcttt aggttgtcaa acggcaagac aagctgttaa
     2161 aatcctcaaa ggtaaagacg tcgctaaagt tccttcagaa tatcctcaaa atctaaaagt
     2221 agtagttaat gaagatatgg caaaagagct tggaattgat gtttcaagta ttaaaaataa
     2281 taaataatca gaaaggtatt gtatgttaga tattatctta tccggaattt cgcaaggatt
     2341 actttggtca attatggcaa ttggcgtgtt tatcactttt cgtatcttag acatagccga
     2401 tctctctgca gaaggggctt tccctatggg ggctgcagtt tgcgccttat gtatcgttaa
     2461 tgatattaat ccaattgtgg caaccatagc gggcatgtta ggtggtatgt tggcaggact
     2521 agtatcgggt tttcttcata ctaaaatgaa aataccagcc ctcctaacag gtattattac
     2581 gttgacagga ctttactcta ttaatttact tgttttagga agatcaaacg tctcttttgc
     2641 tctcaaaaat actttggtta caatggtaac aagattaggt ttaaataaac tttcagcagt
     2701 tttattaatt ggaattgttt gtgtaggttt agttatctta attttgtatc tctttttaaa
     2761 tacccaacta ggattagctt taagagcgac aggtgataat gaggctatgg gacaagccaa
     2821 tagtattaaa gtagatcgga tgaaaatgct aggatatatg attgggaatg gcctgattgc
     2881 tttatctggt gcacttttag cgcaaaataa tggctatgca gatttgaata tgggagtggg
     2941 tacaatcgtt attggattgg cttcaattat cttggcagaa gtaatgataa aatacttacc
     3001 tcttggtaaa aggttatggt caattgtatt aggttctgtt ttataccgta tgattattgt
     3061 tttcatatta acaaccgata ttgatgcaca gatgataaag ttggtttcag caattttgtt
     3121 ggctctcatt ttgtatgtcc cagaattacg agctaaacta ggagtaaaag cctctaaaac
     3181 gcttacaaag gaaggataag aacatgtcat tacttgaatt agtcaatctg cacaaaactt
     3241 ttgaaaaagg aactgtcaat gagaaccatg ttttacgtgg tttagattta acgattgaag
     3301 acggtgattt tatttcagta attggaggta acggtgcggg aaaatcaacc ctccttaact
     3361 gtatcgctgg tctgatccct attgatcagg ggacaatcac attagacaac caatcaatta
     3421 ctaaagactc tgtagaaaaa cgatctaagg gtattagtcg agtctttcag gatcctagaa
     3481 tgggaactgc aacaaatttg acgattgaag aaaatatggc aattgcacat aaaagaggca
     3541 ataagcgtca catttttaga cagtcagtga ctgacgatga ccgtcaacta tttaaaaagt
     3601 ctctatcaca attaggtcta ggcttagaaa atcgaatgaa gactgatgct gcatttttat
     3661 ctggcggtca gcgtcaagcc ctcacgttag ctatggcaac tcttgttcgt cccaaattat
     3721 tattactgga tgaacataca gcggctctag atcccaaaac tagcgatatg gttatggaat
     3781 taacacagaa agtgatagaa gaacaacgat tgacagcctt aatgattaca cacaatatgg
     3841 agcatgccat tgcttatggt aatcgtctcg tgatgctcta ccacgggaaa attgttgttg
     3901 atgttaaagg agaagcaaag aggaacttaa cagttgcaga actaatggaa cttttccata
     3961 aaaactcagg tcaacagttg atagatgatg cccttgtctt aggttaaaat ataaatttct
     4021 ctagtgaatg gacaggataa ctagggaaat ttttcttgtt tgaaacgtgc cttttctctt
     4081 gactaattct gaccaagtga tagaatagtt tttgtagtta gcactctctt tataggaatg
     4141 ctaaaaaatc aaaatatatc tcgtatggag gttcgtttat tatgttaaaa ccattaggtg
     4201 atcgtgtgat catttcgttt gttgaaactg aagagaaatc tgtcggtggt tttgttcttg
     4261 caggagcaag ccatgatacg acaaagacag ccaaagttct tgctgtgggt gacggtattc
     4321 gtaccttaac aggggaatta gttgcaccta gcgttgcaga gggtgatact gttttagtcg
     4381 aaaatggtgc tggtttggaa gttaaagacg gtaacgaaaa agtgacagtt gttcgcgaaa
     4441 gtgacattgt tgctgtcgtt aaataggttt tattaagcaa gaaaggatat aggcctatta
     4501 gagaaaagtt gctttgtaaa gaaagtagac tttgttaaat tttaggcctg ggtattaaat
     4561 tatggcaaaa gatattaaat tttcagcaga tgcccgctca gcaatggtgc gtggtgttga
     4621 tattttagct gatacagtca aagtaacatt aggtcctaaa ggccgtaatg ttgttcttga
     4681 aaaagcattt ggttctcctt taattacaaa tgatggtgtg acaattgcta aagaaattga
     4741 gctagaagat cactttgaaa atatgggagc taaacttgtg tcagaagtgg cttcaaaaac
     4801 taatgatatt gcaggggatg gcactacaac tgctactgtt ttgacccaag ctattgtacg
     4861 ggaaggtctt aaaaatgtaa ctgcaggggc aaatccgatt ggcattcgtc gtggtattga
     4921 aacagctgtt tcagcagcag ttgaagagct aaaagagatt gcacaaccag tttcaggcaa
     4981 agaagctatt gctcaagttg cggctgtgtc ttcacgttct gaaaaagttg gggaatacat
     5041 ttctgaagct atggagcgcg tgggtaatga tggtgttatc actattgaag aatcgcgagg
     5101 tatggaaaca gagcttgaag ttgtggaagg aatgcagttt gaccgtgggt acttgtcaca
     5161 gtatatggta actgataacg agaaaatggt ctctgaactt gagaatccgt atatccttat
     5221 tacagataag aaaatttcaa atatccaaga aattttacca ttattagaag aggttcttaa
     5281 aacaaatcgt ccgttgctaa tcatcgctga tgatgttgat ggagaagctc tcccaacgct
     5341 tgttcttaac aaaattcgtg gaactttcaa tgtcgtagct gttaaagcgc ctggatttgg
     5401 tgatcgtcgt aaagccatgc tggaagatat tgctatccta acaggaggaa ctgtcgttac
     5461 tgaagacctt ggtttagact taaaagatgc tactatgcaa gttttaggac agtctgctaa
     5521 agtaacagta gataaagatt ctactgttat tgtcgaaggt gccggtgact catcagcaat
     5581 tgctaatcgc gtagccatca ttaagtcaca gatggaggct acaacttctg attttgatcg
     5641 tgaaaaatta caagaacgac ttgctaagtt agccggtggt gtagcagtaa ttaaagttgg
     5701 tgcggcgact gaaacagaat taaaagagat gaaacttcgt atcgaagatg cgttaaatgc
     5761 aacgcgtgct gcagttgaag aaggtattgt ttcaggtgga ggtacggctc ttgtgaacgt
     5821 tattgaaaaa gtagcggcac tgaaacttaa tggtgatgag gagactggac gtaatattgt
     5881 tcttcgtgct ctcgaagagc ctgttcgtca aattgcttac aatgctggat atgaaggttc
     5941 agttattatt gaacgtttaa aacagtctga aattggtaca ggatttaatg cggctaatgg
     6001 agaatgggta gatatggtta ccacaggtat cattgatcct gtcaaagtaa cacgttctgc
     6061 acttcaaaat gcggcatctg tagcaagtct tatcttgact acagaagcag tagtagcaaa
     6121 taaacctgaa ccagaagctc ctacagctcc tgcaatggat ccatctatga tgggtggctt
     6181 ctaatcacta acttatcaca acataaaact cccctttaac aaaggggagt tttatgttgt
     6241 gataatagag atatgatagt cactaccact tgcggtaaga agatcataag gataggccat
     6301 ctaatatttg ccagtagtgg gaggaaaaaa gtagcaaaac aatgattgct attatcttag
     6361 attgagattg ataagctaag aattgaatta tagctaatag aattaagtaa ctaataagta
     6421 taaataacat aaaaaagagc attccgtacc cagcacccat tgacggttcc tttggagggg
     6481 ctagaaaata acttaactta aaataagcgc caactattag gcccataatt aattcggtaa
     6541 aagttatgaa aaaagcaact tttaatagtc tattttgcat aggcttgcct ccttaatgag
     6601 aggtaatgga caattcaaag tgctcaatgg gaacgtaatg cttattacac attagtattt
     6661 tctcttggtt aaaaattgtt tcttgtatta atatgaattg tccatgatcg gatagggtgt
     6721 atttagttgc tgaaaaattg ccagatgtcg catatcccaa atggctttgt gagtggcttg
     6781 atatgccttc gtgatagata ccttcttcaa tgaagtttaa caactgttta gtatcatgta
     6841 aagaaatctc agcatctgaa ataagttcga tagggataaa agaaagtgtg taagctaatg
     6901 gtccatcatc ggtgtggtac caacgatcag caataacaac tacaggggtt tcttgtttaa
     6961 gtgaagctgt gatagcttct gcaggaacct ctacacgaaa ttctaattcc acttctgtaa
     7021 ttttggaagt tagacaagtt ttaatggggt gttgtctatt ttcgtaaccg ttttcagaca
     7081 agttgctaga attttctcta atgaaattac cttttcctct gatattttta accaaatggt
     7141 cttcttgtaa tagtgctaat gactttcgta aagtagctcg actaacatta agttgccctg
     7201 ctaattcggg ctcagaaggc aattgcattc caggagaata agtaccgtct tgaatcatgt
     7261 taaaaatctt attgtagact tgcacatgct ttagtttatt tagtttatta tttagttcat
     7321 ttttaggcat tgtaacctcc gtatacaagt tagtttaatt gaaaaaatta gaaaaatcaa
     7381 gttaaaaaat aaaacgtttt ctttcgataa aagcgtttac aaaggttgtc ttgtatgtta
     7441 ttatacaagt gtaaaaaaaa tagacaagac attaaggagg tctttatcat gcagaattat
     7501 tcaggtgaag tcggattgca atatcaccta cagattcgtc caggtgatgt aggtcgttat
     7561 gttatcatgc caggtgatcc aaaacgttgt gcaaaaattg cagaacattt tgataatgca
     7621 gttcttgtag cagacagccg tgaatatgtt acttatacag gtacattaaa tggtgaaaaa
     7681 gtcagtgtta cttcaactgg tattggtggt ccatcagcgt ctattgcgat ggaagaattg
     7741 aaactctgtg gcgctgatac ctttatccgt gttggaacat gtggtggtat tgaccttgat
     7801 gttaaaggcg gagacatcgt tattgcaact ggagctatcc gtatggaagg tacaagtaaa
     7861 gaatatgctc cgattgaatt tcctgctgta gctgacttgg aggtaacgaa tgctctagtt
     7921 aatgcagcta aaaagcttgg ttacacaagt cacgcaggag tagttcaatg taaagatgca
     7981 ttttatggac aacatgagcc agaacgtatg ccagttagtt atgaattatt aaataaatgg
     8041 gaagcatgga aacgtctggg aacaaaagcg tctgaaatgg aatctgcagc tctttttgta
     8101 gcagcaagtc atctaggagt tcgttgtgga tcagatttcc ttgttgttgg taatcaagaa
     8161 cgcaacgctt tgggtatgga taacccaatg gctcatgaca cagaagcagc tattcaagtt
     8221 gctgttgaag cactccgcac acttattgaa aatgataaat cacaataagc gaggttttgg
     8281 aagtcatcat gcaatttatt tatagtatta ttggtatttt attggtatta ggaattgtgt
     8341 atgcaatttc tttcaatcgt aagagtgttt ctctaagttt aattggaaaa gctcttatcg
     8401 ttcaattcat tattgcgcta atcttagtac gtatcccact aggccaacaa gttgttagtg
     8461 ttgtttcaac tggagttact aaagtaatca actgtggtca agctggttta aattttgtgt
     8521 ttggttcatt agcagatagt ggaacaaaaa ctggttttat tttcgccatt caaacgcttg
     8581 gtaatattgt tttcttatct gccctagtta gtctacttta ttatgtagga atccttggat
     8641 ttgtagtaaa atggataggt aagggtgttg gtaaaattat gaaatcctca gaggttgaga
     8701 gttttgttgc cgtagctaat atgtttcttg gtcaaacaga cagtccaatt ttggttagca
     8761 aatacctagg tcgtatgact gatagtgaga taatggttgt gttggtatca ggtatgggaa
     8821 gtatgtcagt ttctattctt ggtggctaca ttgcattagg cattccaatg gaatatctct
     8881 tgattgcttc aacaatggtt cctattggca gtattctcat tgctaaaatc ttatttcctc
     8941 aaacagaacc tgttcaaaaa attgatgaca ttaagatgga taataaaggt aataacgcca
     9001 atgtgattga tgcaatcgct gagggtgcaa gcacaggtgc acaaatggct ttctcaattg
     9061 gtgctagttt gattgccttt gttggtttag tttctttgat taatatgatg ttaagtggat
     9121 tgggaattcg cttagaacaa atcttctcat atgtttttgc tccatttggt tttcttatgg
     9181 gatttgacca caaaaacatt cttctagaag gaaaccttct tggaagtaag ttgattttaa
     9241 atgagtttgt ttcgttccaa caattgggtc acctaatcaa atctttagat tatcgtacag
     9301 cattggtagc aactatttca ctctgtggtt ttgctaattt atcaagttta ggtatttgtg
     9361 tttcaggtat tgctgttctt tgtccagaga aacgtggcac cctagctcga cttgttttcc
     9421 gtgcaatgat tggtggtatt gctgtaagta tgcttagcgc ctttatcgtc ggtattgtaa
     9481 ctctattcta aaagttgaca aaagaaagaa ggatagtaac gtggaagtaa aagatatttt
     9541 aaaaacggta gaccatactt tgctagcaac aacagcaaca tggccagaaa tccaaacaat
     9601 tttagatgat gcaatggctt atgaaacagc ttcagcatgt attccagcct cttacgtcaa
     9661 aaaagcagca gagtacgttt caggtaaatt agctatttgt actgttattg ggttcccaaa
     9721 tggctatagt acaactgcag cgaaggtttt tgaatgtcaa gatgctatta aaaatggtgc
     9781 tgatgaaatt gacatggtca ttaatttgac agacgttaaa aatggggatt ttgatactgt
     9841 tgaagaagaa attcgtcaaa tcaaagctgc atgtcaagac catatcttaa aagttatcgt
     9901 tgagacatgt caattaacta aagaagaact tatcgaactt tgtggagttg tcacacgttc
     9961 aggtgcagac tttattaaaa cctctactgg tttttcgaca gcaggtgcta catttgaaga
    10021 tgttgaagtg atggcaaaat atgtcgacga aggtgttaaa attaaggcag caggtggaat
    10081 ctcatcattg gaagatgctg aaaaatttat tgctttagga gcttcacgct tgggtactag
    10141 ccgtatcatc aagattgtta agaaccaaaa agttgaagaa ggcacttatt aaataatata
    10201 aaggaaatag gaaggatgct ggtctgactt cctattttca attgataaag gagaagttat
    10261 gtcacaattt gatcgcattc acttagttgt cttagattca gtgggtattg gagcggcacc
    10321 agatgccaat gattttgtca atgcaggagt tcctgatggt gcttcagaca cgcttggcca
    10381 catttcaaaa acagttggac ttgcggtgcc aaatatggct aaaattggac ttggtaatat
    10441 tcctcgtccc caagcgctta aaaccgttcc tgcagaagaa aatccaagtg gctacgcgac
    10501 gaagttacaa gaagtatcac ttggtaaaga tacaatgaca gggcattggg agatcatggg
    10561 cttaaatatt acagagccat ttgatacttt ctggaatggt tttccagaag atatcatcac
    10621 aaagattgag gatttttcag gacgtaaagt tattcgtgaa gcgaataaac cttattcagg
    10681 gacagctgtt attgatgact tcggaccacg ccaaatggaa actggtgagc tcatcatcta
    10741 tacctcagct gacccagtcc tacaaattgc agctcatgag gatatcatcc ctctagaaga
    10801 actttaccgt atttgtgaat atgctcgtag tatcacaatg gagcgtccag ctctccttgg
    10861 tcgtattatc gctcgtccat atgtaggtga cccaggcaac tttacgcgta cagccaaccg
    10921 tcacgactat gcagtatcac catttgaaga taccgtatta aataaactag atcaagctgg
    10981 catcgatact tatgctgttg gtaaaatcaa tgatattttc aatggttcag gcattaatca
    11041 tgatatggga cataacaaat cgaatagtca tggtattgat actttgataa aaacaatggg
    11101 gttatcagag tttgaaaaag gcttctcctt tactaatttg gttgactttg atgctctcta
    11161 tggtcaccgt cgtgatcctc atggctatcg tgattgtctt catgagtttg atgagcgcct
    11221 cccagagatc atttcagcaa tgtgtgacaa agacttactt ttgatcactg ccgatcatgg
    11281 taatgaccca acttatgcag gtacagacca tacaagagaa tacattcctc tcttagccta
    11341 tagcccatct ttcacaggaa acggccttat cccagtaggg catttcgcag atatttcagc
//
LOCUS       DATRME010000059        11080 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRME010000059 DATRME010000000
VERSION     DATRME010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..781
                     /locus_tag="VBC23_001552"
     CDS             <1..781
                     /locus_tag="VBC23_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6843488.1"
                     /translation="ADMSPDKVEQKTKFKNISLSSNKLLAKENTMSVLWYQNSAEAKA
                     LYLQGYNVAKMKLDDWLQKPSEKPYSIILDLDETVLDNSPYQAKNIKDGSSFTPESWD
                     KWVQKKSAKAVAGAKEFLKYANEKGIKIYYVSDRTDAQVDATKENLEKEGIPVQGKDH
                     LLFLKKGMKSKESRRQAVQKDTNLIMLFGDNLVDFADFSKSSSTDREQLLTKLQSEFG
                     SKFIVFPNPMYGSWESAIYQGKHLDVQKQLKERQKMLHSYD"
     gene            916..2112
                     /locus_tag="VBC23_001553"
     CDS             916..2112
                     /locus_tag="VBC23_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6843489.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            2375..2914
                     /locus_tag="VBC23_001554"
     CDS             2375..2914
                     /locus_tag="VBC23_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6843490.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            2994..3365
                     /locus_tag="VBC23_001555"
     CDS             2994..3365
                     /locus_tag="VBC23_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6843491.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            3399..4745
                     /gene="glnA"
                     /locus_tag="VBC23_001556"
     CDS             3399..4745
                     /gene="glnA"
                     /locus_tag="VBC23_001556"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6843492.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            4893..5402
                     /locus_tag="VBC23_001557"
     CDS             4893..5402
                     /locus_tag="VBC23_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6843493.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(5560..7239)
                     /locus_tag="VBC23_001558"
     CDS             complement(5560..7239)
                     /locus_tag="VBC23_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6843494.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(7293..7523)
                     /locus_tag="VBC23_001559"
     CDS             complement(7293..7523)
                     /locus_tag="VBC23_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6843495.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            7702..8394
                     /gene="tsaB"
                     /locus_tag="VBC23_001560"
     CDS             7702..8394
                     /gene="tsaB"
                     /locus_tag="VBC23_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6843496.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            8396..8803
                     /gene="rimI"
                     /locus_tag="VBC23_001561"
     CDS             8396..8803
                     /gene="rimI"
                     /locus_tag="VBC23_001561"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6843497.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            8879..9889
                     /gene="tsaD"
                     /locus_tag="VBC23_001562"
     CDS             8879..9889
                     /gene="tsaD"
                     /locus_tag="VBC23_001562"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6843498.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            10009..11034
                     /locus_tag="VBC23_001563"
     CDS             10009..11034
                     /locus_tag="VBC23_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6843499.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYGDGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
BASE COUNT         3586 a         1748 c         2260 g         3486 t
ORIGIN      
        1 agctgatatg tctccagata aggtggagca aaagacgaaa tttaaaaata tttctctaag
       61 ttctaataaa ctgttggcta aggaaaacac tatgtctgtc ctttggtatc aaaattctgc
      121 agaagccaag gctttatatt tacaaggtta taatgttgct aaaatgaagt tagatgattg
      181 gttacaaaag cccagtgaaa aaccatattc aattatctta gatttagatg aaacagtttt
      241 agataatagc ccatatcaag caaagaatat taaagatggc tctagtttca cgccagagag
      301 ttgggataaa tgggtgcaaa agaaatcagc taaggctgtt gcgggtgcca aagaattttt
      361 gaagtatgct aatgaaaagg gaataaaaat ttattatgtc tcagatcgta cagatgctca
      421 agttgatgcg actaaagaaa atttagagaa ggaaggtata cctgttcaag ggaaagacca
      481 cttgcttttc cttaaaaaag gaatgaaatc taaagagagt cgccgtcagg cagttcaaaa
      541 agataccaat ttaattatgc tttttggaga taatttagtt gattttgctg atttttctaa
      601 atcatctagt acagatagag aacaactact aactaaactt caaagtgagt ttggtagtaa
      661 atttattgtt ttcccaaatc ctatgtacgg ttcttgggaa agtgctattt atcaaggaaa
      721 acatctggat gttcaaaaac aattgaaaga acgacaaaaa atgttgcatt cgtatgatta
      781 ataagtatac gaattctctt aaaaacaact aggaaaatct atgaaatcta tttgtgaaaa
      841 agtaatcaat aattacaact gttcacaaaa atatgttata ataaataata tcaaattata
      901 taaggagttt tactaatggc taaattgact gttaaagacg ttgatttgaa aggtaaaaaa
      961 gtcctcgttc gtgttgactt taatgtgcct ttgaaagacg gcgttatcac taacgacaac
     1021 cgtatcactg cggctcttcc aacaatcaag tatatcatcg aacaaggtgg tcgtgctatc
     1081 ctcttctctc accttggacg tgttaaagaa gaagctgaca aagaaggaaa atcacttgca
     1141 ccggtagctg ctgatttagc tgctaaactt ggtcaagatg ttgtattccc aggtgttact
     1201 cgtggtgcaa aattagaaga agcaatcaat gctttggaag atggacaagt tcttttggtt
     1261 gaaaacactc gttttgaaga tgttgacggt aagaaagaat ctaagaatga cgaagaactt
     1321 ggtaaatact gggcttcact tggagatgga atcttcgtta acgatgcatt tggtacagca
     1381 caccgtgctc atgcatcaaa cgtaggtatt tcagcaaacg ttgaaaaagc tgtagctggt
     1441 ttccttcttg aaaacgaaat tgcttacatc caagaagcag ttgaaactcc agaacgccca
     1501 ttcgtagcta ttcttggtgg ctcaaaagtt tctgataaga ttggtgttat cgaaaacctt
     1561 cttgaaaaag ctgataaagt tcttatcggt ggtggtatga cttacacatt ctacaaagct
     1621 caaggtatcg aaatcggtaa ctcacttgta gaagaagaca aattggatgt tgctaaagac
     1681 ctccttgaaa aatcaaacgg taaattgatc ttgccagttg actcaaaaga agcaaacgca
     1741 tttgctggtt atactgaagt tcgcgacact gaaggtgaag cagtttcaga agggttcctt
     1801 ggtcttgaca tcggtcctaa atcaatcgct aaatttgatg aagcacttac tggtgctaaa
     1861 acagttgtat ggaacggacc tatgggtgtc tttgaaaacc ctgacttcca agctggtaca
     1921 atcggtgtaa tggacgctat cgttaaacaa ccaggcgtta aatcaatcat cggtggtggt
     1981 gattcagcag cagctgctat caaccttggc cgtgctgaca aattctcatg gatctctact
     2041 ggtggtggag caagcatgga attgctcgaa ggtaaagtat taccaggttt ggcagcattg
     2101 actgaaaaat aatagtcatt ataatttata ttaaaggaca tgctgatgca tgtctttttt
     2161 tagttgataa tgtagagtta ttgaactttc cttgacttca tttatcattt actagttttt
     2221 agatatagtg gtatttggtt gacactgcaa ttttcaaaag tttatgacga tttgatataa
     2281 attggtttat aaaagatata ctagtaataa ataaaacgtt tacaaaaagg gttttatgga
     2341 ttgatatgtt agaatatata gtgaagggat aggtatgaaa acgttaaaaa aattattatc
     2401 taattacaag tttgatatta aaaagtttaa atttggaatg agaacattta aaacaggctt
     2461 atcggtcttt ttagttttat tggtttttca tttatttggt tggaacgggc ttcaaattgg
     2521 tgctctaact gctgtattta gtttaagaga agactttgat aaaagcgtgc actttggttt
     2581 ttctagaatt attggtaata gtataggtgg cttactctcc ttagtatttt ttgcattcaa
     2641 tgagatattt catcaagctt tttgggtcac tttattaatt gttcctattt gtaccatgtt
     2701 atgtatcatg attaatgttg cttgtaataa taaatcaggt attattgggg gaacagccgc
     2761 attacttatt ataactttat ctatcccaag cggagagaca attctatatg tttttgcacg
     2821 tatttttgaa actttttgtg gtgtttttat tgcaatgatg gttaatacag atattgaaat
     2881 acttcggaaa aaactaaaaa ataacaaatt atagtttgat gtcataaaat ctcacatgag
     2941 agttgacatc aaacttttta aagtttataa tggtttaatg aaaggaggcc atcatgaaag
     3001 aaagagaact taggcgtaca atggctgtat tcccgattgg agctgttatg aagttgacag
     3061 atttgactgc cagacaaatt cgctattatg aggatcaagg gttaatcaca cctgaacgta
     3121 ctgaaggtaa ccgccgcatg ttttcactga atgatatgga ccgcttactg gaaataaaag
     3181 acttcattag cgatggtttg catatttctg atattaaaaa tgaatacatg caaagacaac
     3241 ataagtccaa agagaaacaa aagtcattat cagatgccga ggttcgtcga cttttacaag
     3301 atgagctccg taatcaaggg cgtttttcaa gtccttcaca gcacattgga aatatgcatt
     3361 tgtaaacatg cagtcaagct agatattaag gagactatat gactatcaca gcagaagata
     3421 ttcgtcgcga agttaaggaa aaaaatgtta ccttcctccg tttgatgttc acagatatct
     3481 taggagtaat gaaaaatgtc gaaattccgg ctacagatga acaattagac aaagttttat
     3541 caaataaagc aatgtttgat ggatcttcta ttgagggctt tgttcgtatc aatgaatctg
     3601 atatgtattt atatcctgat ttagatactt ggattgtctt tccttgggga gatgaaaatg
     3661 gagctgttgc aggtctaatc tgtgatatct atacagcaga aggagaacct tttgccggag
     3721 atccacgtgg aaatcttaag cgtaatatga aacgtatgca agagatgggc tataagtcat
     3781 ttaacttagg accagaacct gaatttttcc tatttaaaat ggatgaaaat ggtaacccaa
     3841 cacttgatgt taatgacaaa ggaggctact ttgacttagc tccaacagac ttagcagata
     3901 atacacgtcg tgaaattgtt aatgtcttaa cacaaatggg ctttgaagtt gaagctagtc
     3961 atcatgaagt agcagttgga cagcatgaaa ttgactttaa atatgatgat gttttaaaag
     4021 cctgtgacaa cattcaatta tttaaattgg ttgttaaaac aattgctcgt aagcatggtt
     4081 tatatgctac ttttatggca aaacctaagt ttggtatcaa tggttcaggt atgcattgta
     4141 atatgtctct ttttgataat gaagggaaca atgctttctt tgatccagaa gatccacgtg
     4201 gaatgcaatt atcagaggat gcctactact ttttaggtgg gttaatgaaa cacgcttaca
     4261 attacacagc tatcattaat ccaactgtta attcttataa acgtttggta ccaggatacg
     4321 aagcaccagt ttacattgct tgggcaggtc gcaatcgttc gccacttatt cgtgtacctg
     4381 catctcgagg tatgggaaca cgcctagaat tgcgctcggt tgacccaaca gcaaatcctt
     4441 atcttgcttt atcagtactt ttgggatcag gacttgaggg aatcgagaat aaaattgaag
     4501 caccagaacc aattgagaca aatatctatg ctatgactgt agaagaacgt cgtcaagcag
     4561 gtattgttga tttaccatct actcttcata atgctcttaa agcacttgaa gaagatgaag
     4621 tggtaaaagc ggccctagga actcatatct atactaattt cttagacgct aagcgtattg
     4681 aatgggcaag ttatgcgact tatgtttcac aatgggaaat tgataattac ctagatttat
     4741 attaatcata agaatgttta taactagttt taggctttat tttgtaaagt ctaaaactag
     4801 tttttttgta aattattgta aaaaattgcc taaaacttgt tacaggtttt catttgtctg
     4861 ttatatggat ttgataaact aggagtcatc agatggagga aaacatgaac ataaaacaat
     4921 taaagtctaa aacaatgctt ggaacagtgg ctttggtgtc cgctttttca tttgcttcaa
     4981 caaatgcaga tgcgaataca tataactatg cagtagatgt agactaccta gcaagtgcgg
     5041 aggaaattgc tcaagcacat ccagctagta atacgtttcc actaggtcaa tgcacatggg
     5101 gtgtaaagga gatggcaaca tgggcaggta actggtgggg aaacggtggt gattgggctg
     5161 cgagcgccgc atcagctggt tatacagtag ggacacaacc gcgcgtgggt tctatcgtat
     5221 gttggactga tggtagttat ggacatgtgg cttatgttac agctgtagat cctgtcacta
     5281 ataaaattca ggttttagaa tctaattatg caggtcatca atggattgat aattatcgtg
     5341 gttggtttga tccacaaaat acagtaacac caggggtagt tagctacatt tatccgaatt
     5401 aatctataaa gaactcctca catagacggt ataaaagtac gatactgttt ggagttcttt
     5461 tatattctat agttatccca gaagtaattt gatatattaa cttattttgc taatataggc
     5521 aattaaaaag aacggagaaa gtattttccg ttcttttgtt tatttatcag gggtaaggac
     5581 cataggaata atgataggct cacgctcagt tttttcatat aagaatgggc gtaaggcatt
     5641 aacaatagct ccatttactg attggatact tgcatcttta tttttcagtg caatacgaat
     5701 agcattgaat aagatatgtt ggctttcgcg aataaggtcc ccagattctc tcatatagat
     5761 aaagcctcgg ctaaggatgt ctggtccagc taaaatcatt tttgaatcga aatcaacggt
     5821 agcaacggca aggacaacac catcttcaga taagtcgtgg cggtcacgta gaactgccgc
     5881 accaatatcg ccaataccat tcccatcaac gtagatatct tgagcattga aatgtccagc
     5941 aatacgagca gagtcgctcg ttaaagctaa aacatcaccg ttttccataa tgaagatatt
     6001 ctctttagga atacctgtgt caactgctaa accagcatgg actttttgca tgcgatactc
     6061 accgtgtacg ggcatgaaat actttggttt aatcaagcgt aacattaatt tttgttcttg
     6121 ttgaccacca tggccggagg tatggatatt gttaatttta ccatgaataa catcgacacc
     6181 agcttcttga atggtattga ttaatttatt gacacttgtt gtattaccag ggattgggct
     6241 tgatgagaaa atcacagtat caccaggctg caaagtaact tgacgatgag taccattagc
     6301 gatacgagca agtgctgcca tagactcacc ttgactacct gtacacataa ttaaaacttc
     6361 actagcatga agatttttaa gttcgcttgg ctcaatgaag gtaccttttg gaactttaat
     6421 atagccaagt tcaataccgt taacaattgc cttttccatt gaccgtccaa aaacagcaat
     6481 tttacgacca gttttaacag cagcttcagc agcctgttgg agtcggaaga tgtttgaagc
     6541 aaaagaggcg aaaataatac gtccctcgat accttcgata atcttcataa tagattgccc
     6601 aacaattttt tcggaattag tgaaggttgg aacttcagca tttgtcgagt ctgataaaag
     6661 gcagaggaca ccatcttcac ctagagcagc catacgatgt aagtcagcgg gctccccaac
     6721 aggtgtaaag tcaaatttga agtcacctgt acaaataacc ttaccttgag gggtatgaat
     6781 aacaatacct aatggttcag gaatggagtg agtagtgcgg aaaaatgtaa cgcttaagtt
     6841 tttaaaagtc agctctgtat tagcgtggat ttcgtataat gtagcgtcac gaagtaaacc
     6901 atgttcttct aacttacctt tgattagagc aagagcgaga ggacctgcat aaatcggtaa
     6961 attagcttgt tttaatagaa aaggaatacc accgatgtgg tcctcgtgcc catgagttat
     7021 aacaagggcc ttgatacggt caatgttttc aacgatatat gaatagtctg ggataacata
     7081 atctattccc aaaagatcat cttcagggaa tttaatacca gcgtctacaa taataatttc
     7141 gtcttgatat tcgataccgt aagtattttt tccgatttca ccgagaccac caatcgcata
     7201 aaccccaact tcttcgggtt ttaagttgat atttgacata ataagtttat cataatgggt
     7261 agattactct agctattatg tttaactcct ttctagaact cagtgagctc gaaaacacca
     7321 gtttcttttt catattcaag gtgtttgtct gaaagtaact cgatgaattc gatattgtaa
     7381 gctgtatttt cttcaactag tttacgtgca gcaatgcgac cttctaattc attagctgca
     7441 tcaatagtaa cgtaaagtgt tttggtttgc tcacgacgtg ggttgcgttc ttttgtttct
     7501 tgatagaaaa ctttataaat cataataatc ctttcttatc tctcggtttt tctaactata
     7561 aaaaaatggg tcaatgccgt taagtgagac aatgacaaac cattattaag tttttataga
     7621 gtaatagaat taaccttgat tcgttgcaat tatcatgatt atatcataaa ataagaggta
     7681 gaatcaagta agggaagtta aatgatgaaa gttttagcct ttgatacttc aagcaaagca
     7741 ctatcagtgg ctgtactaaa caatatggaa tgtttagcga ctgtcactat caatatcaaa
     7801 aagaatcata gcattaattt gatgccagcc attgattttt taatgcaatc aattgattta
     7861 gaacctcaag atttggaccg tatcgtagta gcagagggtc caggatctta tacgggctta
     7921 cgtgtagctg ttgctacagc aaaaatgcta gcttatacgc ttaagattga cttagttgga
     7981 gtatctagcc tgtacgcttt aacaaatgga ttttcagaaa atgatttatt ggtaccactt
     8041 atagatgcac gacgtaacaa tgtttatgtt ggtttctatc aaaatggtga tactgttaaa
     8101 ccagactgtc acacttctct tgaagaagtc ttacaagagg tggggaataa agccaatgtt
     8161 cattttgtcg gagaggttgc agcatttttt gatcagatta agaaagcctt accacatgct
     8221 aaaattacag aaactttacc ttgtgcagta gcaattgggc gcaaaggaca aaaaatgaaa
     8281 agcgttaatg tagatgcgtt tgttccacga tacttaaaac gtgttgaagc tgaggaaaat
     8341 tggttaaaaa accactgtga aacgaataca gaagaatata ttaagagagt ttgacatgga
     8401 atcatcagaa caagcactgg ctatatggag cgtactatct gatgtctatg acaaatctcc
     8461 gtggagttta tcgcagattt cagaagattt aaaaaaagat tcaacagatt atttttttgt
     8521 ttataatgac ggtgaagtca ttggtttttt ggctcttcaa caattggtag gtgaagtaga
     8581 aattactaat attgctgtta aaaaaaatta tcaaggtaaa ggttatgctt accagttaat
     8641 gtcaatgatc gccgatatag aggtgccagt ttttcttgag gttcgttatt caaatatcgt
     8701 agcacaaaaa ctttatgaga ggtgtggttt tgtagtttta agaaagcgca aaaattatta
     8761 ccatgatcca attgaagatg ctatagtgat gcgcaaggga taaatagttg atgatagaaa
     8821 ggatatgaat agtcctcaat actaatggaa aagacgatat tgatggatta atatcattat
     8881 gaaagataga tatattttag cagtggaaag ttcatgtgat gaaactagtg ttgctatttt
     8941 aaaaaatgat aaagagttac tagctaatat tattgcaagt caagttgaaa gtcacaaacg
     9001 ttttggtggt gttgttcctg aagtggcaag ccgtcatcac gttgaagtag taacaacctg
     9061 ttttgaggat gctcttcaag aagcaggtat tgttgctagc gatttggatg ctgttgctgt
     9121 aacatatggt ccgggattag taggagcctt attggtaggt atggctgcag caaaagcttt
     9181 cgcttgggca aataaattac ctctaattcc tataaaccac atggcaggtc atttaatggc
     9241 agcacgtgac gttaaggaac ttcaataccc attgttagct ttgcttgtca gtgggggaca
     9301 tacagaatta gtatatgttt ctgaaccggg agattacaaa atagtaggag aaactcggga
     9361 tgatgctgtt ggagaagctt atgataaagt aggccgtgtt atgggcttaa cttatccagc
     9421 aggtcgcgag attgatcagt tagctcataa gggtcaagat acttaccatt ttcctagagc
     9481 gatgatcaaa gaagatcatc ttgaattttc tttttctgga ttaaaatctg catttatcaa
     9541 tttacatcat aatgcagaac aaaagggtga agcattggtt cttgaagatt tatgtgcttc
     9601 ctttcaggcg gctgttttgg atattttatt ggccaaaact caaaaagctt tgttaaagta
     9661 tccagtgaaa actttagtcg ttgctggtgg agttgcagct aatcaaggac ttcgggaacg
     9721 cttggctact gatatttctc ctgatattga tgtggttatt cctcctctta gattatgtgg
     9781 ggataatgca ggaatgattg cattagcagc agcgatagag tttgaaaaag agaattttgc
     9841 ttctttaaaa ttgaatgcca aacctagttt agcttttgag agtttatagg tgttcataat
     9901 atcaaaacgc ttgttttgat attttttatt tgttataatg attgaatatt caaaaatatt
     9961 agcaaatgat attgtcgcta tttttttaac taataaagga gttaaggaat gttacatttt
    10021 gaaaatgatt ataataaagg agctcatcca gagcttttaa atgcccttat tgaaactaat
    10081 gatcaaggtc tatcgggata tgggacagat agttactgtc aacaagcggc agataaaatt
    10141 cgagaagtct gttcctgtcc acaagctgaa gttgagtttt tagtgggtgg tactcaaaca
    10201 aatcaagtag ttatcagtag tatgcttgct tcttatgaag gagttattgc tgcagaaaca
    10261 ggtcatgtga gtagtcatga agcaggagcc atagaatttt cagggcataa ggtattgact
    10321 ttaccttctc ataatgggaa attactcgca agcgaagtcg caacttatat tgagactttt
    10381 tatggtgatg gtaattacca acacatggtt tttccaggaa tggtttatat atcacatcca
    10441 acagaatatg gaacacttta tagtaaagca gaactagaag agttatcgaa gatttgtaaa
    10501 cattatcaaa tccccttatt tattgacggt gctcgtctgg ggtatggttt ggctgcgaaa
    10561 gatacagatg ttgatttccc aacgattgca gccttatcag atgtttttta tattggtgga
    10621 accaaaatgg gggctcttgc tggggaagca gttgttttca caaagaaaaa tcgtccaaaa
    10681 cagtttacta caattgtaaa gcagcatggt gcgttattag ccaagggaag attgctgggg
    10741 cttgcttttg atcgtttctt tacggataac ctttacttaa aaattggaaa gcatgcgatc
    10801 gacttagcag aggaattaaa aatcatttta gaagaaaaag gttactcatt ctatttaaaa
    10861 tcaccaacta atcaacaatt tattattgta gaaaatacaa aactagcaga tttagcaaag
    10921 aatgtagctt atagtttttg ggaaaaatat gatgaccatc atactgtaat acgtctagca
    10981 actagttggt caactagtcg cgaagatgta actgctttaa gaaatgtctt gtgagttttt
    11041 aataaaagaa atattattca gtagtgacta ttacaaaata
//
LOCUS       DATRME010000060        11072 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRME010000060 DATRME010000000
VERSION     DATRME010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..817
                     /gene="dnaN"
                     /locus_tag="VBC23_001564"
     CDS             <1..817
                     /gene="dnaN"
                     /locus_tag="VBC23_001564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6843500.1"
                     /translation="SGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAF
                     AASQQESRPILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLR
                     EFSAVFTDDIETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFN
                     TNALRHAMERAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSL
                     NISFNPTYLIESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            875..1768
                     /locus_tag="VBC23_001565"
     CDS             875..1768
                     /locus_tag="VBC23_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO6843501.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            1778..1975
                     /locus_tag="VBC23_001566"
     CDS             1778..1975
                     /locus_tag="VBC23_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6843502.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            2178..2294
                     /locus_tag="VBC23_001567"
     CDS             2178..2294
                     /locus_tag="VBC23_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843503.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            2538..3653
                     /gene="ychF"
                     /locus_tag="VBC23_001568"
     CDS             2538..3653
                     /gene="ychF"
                     /locus_tag="VBC23_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6843504.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            3737..4312
                     /locus_tag="VBC23_001569"
     CDS             3737..4312
                     /locus_tag="VBC23_001569"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6843505.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            4309..7806
                     /gene="mfd"
                     /locus_tag="VBC23_001570"
     CDS             4309..7806
                     /gene="mfd"
                     /locus_tag="VBC23_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6843506.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
     gene            8097..8369
                     /locus_tag="VBC23_001571"
     CDS             8097..8369
                     /locus_tag="VBC23_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6843507.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            8356..8727
                     /locus_tag="VBC23_001572"
     CDS             8356..8727
                     /locus_tag="VBC23_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6843508.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            8730..8864
                     /locus_tag="VBC23_001573"
     CDS             8730..8864
                     /locus_tag="VBC23_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6843509.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            8864..10150
                     /locus_tag="VBC23_001574"
     CDS             8864..10150
                     /locus_tag="VBC23_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6843510.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            10152..>11072
                     /gene="tilS"
                     /locus_tag="VBC23_001575"
     CDS             10152..>11072
                     /gene="tilS"
                     /locus_tag="VBC23_001575"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6843511.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFK"
BASE COUNT         3933 a         1576 c         2056 g         3507 t
ORIGIN      
        1 tagtggaaaa tcagagatta ctttgaaagg taaggatgtc gatcaatacc ctcgtctgca
       61 ggaaatgaca acagatactc cattaacatt agaaactaaa ctgttaaaat caattattaa
      121 tgaaactgct tttgctgcta gccaacaaga aagccgtcca atcttaacag gtgttcattt
      181 ggttatcagt caaaataaat actttaaggc tgttgcgaca gattcacacc gtatgagtca
      241 acgcactttc caattagaga aatcggctaa taattttgat ttggttgttc caagtaaatc
      301 ccttcgagaa ttttcggctg tttttacaga tgatattgaa actgtagagg ttttcttctc
      361 agatagtcaa atgttattta gaagtgaaaa tatcagcttc tatacacgtt tgcttgaagg
      421 aaactaccct gatactgatc gcctcctaac taatcagttt gaaaccgaaa ttatctttaa
      481 tacaaatgct ttacgccatg ctatggaacg tgcttattta atttcgaatg caactcagaa
      541 cggtactgtt cgtttagaaa ttcaaaatga aacagtctca gctcatgtaa actctccaga
      601 agttggtaaa gttaatgagg aattggatac tgttagcctt aaaggtgata gtttaaatat
      661 tagttttaat ccaacttacc taattgaatc tttaaaagca gtaaaaagcg aaacagttac
      721 gattcgattt atttctccag tacgtccatt tactttgacc cctggtgaag atactgaaga
      781 tttcatccaa ttaataactc ctgttcgtac taactaaata agaaaactac ccaagttatt
      841 cttgggtagt tttcttattc taagatatgc tataatgatg agtaatatga ctctatatat
      901 aatagctaac ccccatgctg gtaataaaaa tgcctccact attgttggta aaattcagga
      961 gctttatcat actgaagata tttctgtgtt ctatacagaa cagaaagatg atgaaaaaaa
     1021 acaagtcatt aatatactaa ggtcttttaa agaaagtgat catctaatga ttataggagg
     1081 agatggtacc ttatcaaaag taatgactta tctcccccaa catattccgt gcgcttatta
     1141 tcctgttggt tcaggaaatg attttgccag ggctttgaaa attcctaacc tcaaagaaac
     1201 ccttaccgct attcagacag aaagattaaa ggaaattaat tgctttattt atgataaagg
     1261 gctgattttg aatagtttag atttaggttt tgcagcctat gtcgtttgga aagcatctaa
     1321 ctctaaaata aaaaatattt tgaatagata tcgtctagga aaaataactt acattgttat
     1381 agctatcaaa tcattattac attcttctaa agtacaagta ttagtggaag gagagacagg
     1441 acagcggata aaactaaatg acctttattt tttcgcatta gcaaataaca cttattttgg
     1501 aggtggcatt actatttggc ctaaagcaag tgctttaact gcagagttag atatggttta
     1561 cgctaaaggg catacttttc taaaacgatt aagtatttta ttatctttgg tttttaaaag
     1621 gcatactacc tctaaaagta taaaacacca gactttcaaa gctatgaccg tatattttcc
     1681 taaaaatagt ttgattgaaa tagatggcga aatagttgag ttagatcaga ttagtttaaa
     1741 atgtcaaaaa aggtatttat atatgtaagg agaaaagatg tatcaagttg gatcacttat
     1801 tgaaatgaaa aaacctcatg cttgtgtaat taaagaaact ggtaaaaagg ctaatcaatg
     1861 gaaggtactg agagtaggag ctgatattaa aattcagtgt actaactgtc agcatgtgat
     1921 tatgatgagt cgttatgatt ttgagcgaaa attaaaaaaa gtactgcaac catgaaagtc
     1981 cttttaaaca aataattata gctatatttg caaccaatac tttcctaaat aattgctagt
     2041 atgccgttgg aaaattagct attctaatgt tatcgaaaga agaaaggtgg ccattgacaa
     2101 tgacgaaagt tgcagaacag ttgtttattt ttcggtaagt catatcaaaa tgggaaaatg
     2161 gggataccac accagatttg gataatttaa tcaggttagc tgagattttt gacgtgagct
     2221 tagatgaaca tgttttagct aaaacaaatg aagttaaagt tgaacgtatt tatgaaaaaa
     2281 atcttgatct gtaaaaatat aataaaattt attggtttat ttttcgaaat attattcgat
     2341 ctctactaat tatcttagct atattaactt tcttataagc tctaggtctt ccctttgttt
     2401 cggagtggat aatttaaatg aaggtgaaag agtggcataa tgataagcaa atctgaaatt
     2461 ttcttcttat cataccgctt tttcttctat tttttgatat aatggtcttg attgaaattt
     2521 gaatggagac tcactaaatg gctttaacag caggtattgt aggcttacca aatgttggta
     2581 aatcaacttt atttaacgca attacaaaag caggagcaga agctgctaac tatccttttg
     2641 cgactattga tcccaatgtt ggtatggtag aggtaccaga tgagcgtctg caaaaattga
     2701 cagaattgat tacgcctaaa aaaacagttc cgacaacctt tgaatttact gatattgcag
     2761 gtattgttaa aggtgcttcg aagggggaag gtctaggaaa taaattttta gctaatattc
     2821 gtgaagtgga tgctattgtt catgtggtac gtgcttttga tgatgaaaat gttatgcgtg
     2881 aacaaggtcg tgaggatgcc ttcgttgatc ctatagcaga tattgatact attaatcttg
     2941 aattaatctt agctgattta gagtcaatca ataaacgtta tgcgcgtgtt gaaaaaatgg
     3001 cacgaactca aaaagataaa gaatcagtag cagagttcaa tgttttacaa aagattaaac
     3061 ctgttttgga agatgggaaa tcagctcgta ccattgagtt tacagaagag gaagcaaaag
     3121 ttgttaaagg tctcttttta ttaacaacta aacctgtttt atatgttgct aatgttgatg
     3181 aggacaaagt agctgatcct gatgatatcg actatgttaa tcaaattcgt gcatttgcag
     3241 aaacagagag tgcagaagta gttgtgattt ctgctcgagc tgaagaagaa atatctgaac
     3301 ttgatgatga agataagtta gaatttttag aagctattgg attaacagaa tctggtgttg
     3361 ataaattaac acgagctgct tatcatcttt taggccttgg aacttacttt actgctggtg
     3421 aaaaagaggt tcgcgcttgg acatttaagc gtggtataaa agctccacaa gctgcgggta
     3481 ttatccattc agattttgaa agaggtttta ttcgtgcagt aacgatgtca tatgatgacc
     3541 ttattcaata tggatctgaa aaagcagtaa aagaagctgg ccggttacgt gaagaaggaa
     3601 aagagtatat tgttcaagat ggcgatatca tggaattccg tttcaatgtc tagtaataaa
     3661 taatcaattt gttaactttt aggttaagga tattgttatt atccttaacc atttttgcgt
     3721 tttaatggag aagataatgg taaaaatgat tgttggatta ggaaatccag gaagtaaata
     3781 taatgataca aaacataata ttggatttat ggctgttgac agaattgtaa aaaaacttga
     3841 cgtcaacttt acagaagata aaaattttaa agcagagata ggtagtgatt ttatcaacgg
     3901 tgaaaaaatt tattttatca aaccaactac ttttatgaat aacagtggta ttgcggtaaa
     3961 agccctgctt acttactaca acatttctat taaggatatg attatcatat atgatgattt
     4021 agacatggaa gtcggtaaaa tacgttttcg tcaaaaagga tctgctgggg gacataatgg
     4081 tatcaagtct attattgctc atttaggtac tcaagaattt gatcgtatta aagtaggtat
     4141 aggacgtccg aatggacgta tgacagttat taatcatgtt ctagggaagt ttgataaaaa
     4201 tgatgaaatt atgatatcaa atactcttga taaggttgac aatgctgtca attattattt
     4261 acagacgaat gattttcaaa aaacaatgca gaaatacaac ggtttaaaat gaatattata
     4321 gaattattta gccaaaataa agtggtgcgg acatggcact ctggattagt aactaatagt
     4381 cgccaattag ttatggggtt ttcaggagca agtaaagcta ttgctatagc atcagcctac
     4441 gagaagttat ctaaaaaaat tatggtagtc actgccactc aaaacgattc tgataaatta
     4501 tcaagtgata tatcaagttt aattggagaa gataacgttt atcaattttt tgctgatgac
     4561 gttcctgcag cagagtttat tttctcttca ttagacaaat caatttcacg actcagtgcc
     4621 ttgaggtttc taaaagatcc agaaaaaaac ggtgttttga ttactagtat cagtggttta
     4681 aggttactat taccaaatcc ggaagttttc tcgaaaagtc aatataagtt tgaaatcggt
     4741 caagaatgtt atttagataa attgagtaag aacttagtca atttaggtta tcagaaagtt
     4801 tctcaagtat ttagtcctgg tgaatttagc caaagaggag atattttaga tatttttgag
     4861 atgacacaag aatatcctta tcgcttggaa ttcttcggag atgaaataga tggcatcagg
     4921 cagtttgata ttgacactca aaagtctctt aaacaattag agtcagtaca gatttcgcca
     4981 gcagatgata ttattttaca agatgcagat ttcgagcgtg caaagaaaaa acttgagggc
     5041 tatctagtaa cagctagtga ggaacaacgc gcctatttga gcgaggtttt atctacaacc
     5101 gagaatcatt ttaagcactc aaatattcgt cgttttctaa gtatctttta tgaaaaagaa
     5161 tggggaatat tagactatat tcctgaggga actccacttt ttgttgatga ttttcaaaaa
     5221 attgtagatc gaaatgctaa gttagattta gaaatagcta gccttctgac ggaagattta
     5281 caacaaggca agtctcattc tagtttgaac tatttttcag atccttataa gcaattaaga
     5341 caataccaac cagcaacctt cttttcaaat ttccacaaag gattaggaaa tctgaaattt
     5401 gataaacttc atcattttac tcaatatgga atgcaagaat tttttaatca atttccattg
     5461 ttggtcgatg aaatcaatcg ttataagaaa tctggagcga cagtactgtt gcaagtagat
     5521 tctcaaaaag gtttaaatct ccttcaagaa aacttaaaag aatatgggtt agatttaatc
     5581 atttcagata aaaatgatat cgttccaaag gagtcacaac ttattgttgg gcatctttca
     5641 aatggttttt attttgcaga tgagaaaatt gttctgatta ctgaacgtga gatttatcat
     5701 aggcgtgtta aaaggaaaat tagacgcagt aatattagta atgcagaacg tctaaaagat
     5761 tataatgaac tttcagttgg cgattatgtt gttcataatg ttcatggcgt aggaaaattt
     5821 ttaggtattg aaacaattga gatacaaggt attcatcgtg attatctgac tattcaatat
     5881 caaaatgctg atcggatctc tatccccgta gaacaaattg aacttctaac aaaatatgtc
     5941 tctgcagatg gcaaagagcc taaaattaat acgttaaatg atggtcgctt caaaaaagca
     6001 aaacagagag ttgcaaagca agtagaagat attgctgatg atttgttgaa actttatgca
     6061 gagcgtagtc aattacaagg atttgcattt tctcctgacg ataatatgca aaatgacttt
     6121 gataatgatt ttgcatatgt agagacagag gatcaattgc ggtcgataaa agaaattaaa
     6181 caggatatgg aaggtaatcg tccgatggat cgattattgg ttggagacgt tggttttggt
     6241 aagacagagg ttgctatgcg tgcggctttc aaagctgtca atgaccataa acaagttgct
     6301 gttttagtcc caacaacagt tctagctcag cagcattttg aaaattttaa agaacgtttt
     6361 agtaattatc ctgttacagt tgatgtactt agtcgttttc gtagcaaaaa agaacaaaca
     6421 gatacactaa aaagattatc taagggccaa gttgatatta ttattggaac acatcggctt
     6481 ctctctcaag atgttgtatt ttcggattta gggttaatag ttattgatga ggaacagcga
     6541 tttggtgtaa agcataaaga aaagttaaaa gaattaaaaa caaaagttga tgtcttaact
     6601 ttgacagcga ccccaatacc tagaacatta catatgtcaa tgcttggcat tagagactta
     6661 tcagtcattg aaacacctcc aactaatcgt tatcctgtgc agacttacgt tttggagaca
     6721 aatcctggtt tagttagaga agctattatt cgtgagatag acagaggagg acaggttttt
     6781 tatgtttaca ataaagttga cactattgaa cagaaagttt cagaacttca ggagttagta
     6841 ccagaagcta gtattggttt tgttcatggt caaatgtctg aaattcaatt agaaaataca
     6901 ttaatagact ttatcaatgg agattacgat gttttagttg caacgacaat aattgagaca
     6961 ggagtcgata tttcaaatgt taataccctc tttgttgaaa atgcagatca tatgggcttg
     7021 tcaacccttt atcaattaag aggccgtgtg ggtagaagta atcgcattgc ttatgcttac
     7081 cttatgtaca gacctgataa agttttaaca gaaatttctg agaaaagact agatgcaatc
     7141 aaagggttta cagaattggg atctggtttt aaaatagcga tgcgtgattt atctatacgt
     7201 ggtgcaggaa atattttagg agcttcacaa agtggtttta ttgattcggt tggattcgag
     7261 atgtattcac agcttctaga gcaagcgatt gccaccaaac aaggaaaatc attaattcgt
     7321 caaaaaggta atgttgaatt agccttacaa attgacgcct atttaccagc agaatatata
     7381 tcagatgagc gccaaaaaat agaaatatat aagcgtattc gtgaattaga aactagggct
     7441 gattatgaag ccttacaaga tgagttaata gatcgttttg gagaatatcc tgatcaagtt
     7501 gcataccttt tagaaatagg acttttaaaa gcttatctgg atcttgcctt tactgaatta
     7561 gtagaaagaa aaggtaatga aataagcatc atatttgaaa aagcatcttt gaaatatttt
     7621 ttaactcaag attattttga agcactatca aaaactcaac ttaaggctag aattagcgaa
     7681 acaaatggta agatggaagt tgtttttaat atcaaacata agaaaaatta tgaaattatt
     7741 gaagaacttc taaaatttgc cgaatgtttt atcgagatta agtcacgtaa accagtagaa
     7801 gaataactaa gtgaaaagtt gcaaggaaat ttggatactc ttttagaaaa tgtttggttc
     7861 aagaagaata gtacgagaaa cattatgttt cacgtcaaac atctcaactt ttgaaagaca
     7921 taaatatttt tagaggttat taaaatagaa gttttttact tgatatttga gagaattaag
     7981 agagctaata gctctctttt tgttacatta tattgacaaa tattatcagt tcagatattt
     8041 ttcaaaaaaa atgctaaaat agtagagtta atttagatag aaggatgtga tcatcagtga
     8101 gattagacaa gtatctaaaa gtgtcgcgta tcattaagcg tcgtcccgtt gctaaagaag
     8161 ttgcagacaa aggacgtgtc aaggtgaatg gcgtattagc aaagtcatca actgacctaa
     8221 agcttaatga ccaagttgaa attcgttttg gtaataaatt gttgaccgtt aaagtattag
     8281 aaatgaaaga tagcactaag aaagaagatg ctatcaaaat gtatgaaatt attaatgaaa
     8341 caaggataga aacagatgag caagcctaat gttgttcagt taaataatca atatattaac
     8401 gatgagaatc taaaaaaacg ttacgaagct gaggagttac gccgaaaaaa tcgtttaatg
     8461 ggttgggttc ttatttttgt catgctttta tttattttac ccacttataa tttagttaag
     8521 agttacagaa ctttacaaga acgtcgtcaa gaagttgtaa aattaacgaa agactatcag
     8581 acattaacta atagaactga gaaccagaag ttactagcaa aacaactaaa aaatccagat
     8641 tacgttcaaa aatatgctcg agcgaagtat tatttctcta agaccggcga aatgatttac
     8701 ccattaccag accttttacc aaaataatta tgaataattt aattgaaaca atagaacatt
     8761 ttctagccta ctcggatgac aaactagaag agttagctga taagaactat caacttaggc
     8821 aaacaattgt agaaaaagaa aagattgaga gcagagaaaa gtaatgaaga aagttttaac
     8881 cttcttatta tgtagcttat attttgtatc aatcccagct ataagtaccg aggagccatt
     8941 aacgctttct caaaatagac gatacgcctt aactcagaca gttgtggata aggaaatgta
     9001 ttttgatgct attccagaga gaccaactac aaaaattgaa ataagcagtt ttcaagatga
     9061 agctttaacc ataacaggag agactttagt tccaaatact ttattgtcga ttatttccct
     9121 tacaattaat tcaaatggaa ttccagtatt tactttgtcc aatggacagt tcataaaggc
     9181 tagtcgtgaa gctattttta atgatttagt ttctaagcag caatctgtct cattggatta
     9241 ttggttaaaa ccttcttttg tgacttatga agcaccttac acaaatggtg ttagtgaagt
     9301 taaaaataat ttaaaacctt attccagagt gcatttagta gaacaagcag aaacagaaca
     9361 tggtatttat tataaaacgg acagtggttt ttggatatca gtagaagatc tatcggttgc
     9421 tgataatcgt atggctaagg ttcaagaagt attattggaa aaatataata aagataaata
     9481 tggtatatac atcaaacaat taaatacaca gacagtagct ggtattaata ttgataggtc
     9541 aatgtactct gcaagtattg caaaattagc aacactttat gctagccaag agcaagtaaa
     9601 attaggaaaa ctgtcattgg acagcaaatt tgaatacaaa gataatgtta atcaatttcc
     9661 taatagctat gatccaagtg gtagtgggaa attagagaaa aaagctgatc ataaattata
     9721 tacagttaag gagttacttg aagcaactgc caaggaatca gataatgttg caacaaatat
     9781 gctaggttat tatgtcaata atcaatacga ttcaatgttt caaacacaag tagacacaat
     9841 ttctggtatg cactgggata tgaagaaacg ccagatttct ccacaagctg cgggtaaaat
     9901 gatggaagca atttattatc aaaatggaga tattgttaat tacctatcaa agactgattt
     9961 tgataataca agaatcccta aaaatatacc agttaaagta gcacataaaa ttggagatgc
    10021 ttatgactac aaacatgatg cggctattgt ttatgcagaa caacctttta ttatgattat
    10081 ttttacagat aaatcatctt acgatgatat cactaagatt gctgacgacg tttaccaggt
    10141 tttaaagtaa tatgtataac actattttga aagatacctt atccaaaggg ttatttaccg
    10201 ctcatcagaa agttctaatt gctgtttctg gtggcattga ttcaataaat ttgttgcaat
    10261 ttttatacca gtatcaaaaa gagttatcaa ttagtattgg tattgcacac ataaatcatg
    10321 gacagcgaaa agaatcagaa aaagaagaag aatatatcag acagtgggga cagatacatg
    10381 atgtccctgt ctttatctct tattttcaag gaatattttc agaagataga gcacgtaacc
    10441 atcgctacaa ttttttttca aaagttatgc gtgaagaagg ttacacggct ttagtaacag
    10501 ctcaccatgc tgatgatcaa gctgaaactg tatttatgag aattttaaga ggaagcaggc
    10561 tacgctattt atcagggatt aagcaagtga gtgcttttgc aaatggtcaa ttgattagac
    10621 catttcttcc atataaaaag gagttattac caaatatttt tcattttgaa gatgctagca
    10681 atgcttcgtc tgattatttg agaaatcgaa taagaaacat ctactttcct actttggaaa
    10741 gagaaaataa tcaattgaaa gattctctca taactctctc tgaggaaaca gagtgtttat
    10801 tcacggcttt aacagaccta acaaggagca tcgaagtaac aaactgctat gattttttaa
    10861 ggcaaactca ttccgtccaa gaatttttat tgcaagatta cattagcaaa tttccagatt
    10921 tacaagtgag taaagaacag ttcagagtaa tattaaaatt gattagaact aaagccaata
    10981 ttgactatac tattaaatcg ggctattttc ttcataagga ttacgagagt tttcatatta
    11041 caaaaatcca tcctaagacg gatagtttta ag
//
LOCUS       DATRME010000061        11067 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRME010000061 DATRME010000000
VERSION     DATRME010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..144
                     /locus_tag="VBC23_001576"
     CDS             <1..144
                     /locus_tag="VBC23_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6843512.1"
                     /translation="AIGLVFTAKSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWL
                     LLV"
     gene            complement(177..1772)
                     /locus_tag="VBC23_001577"
     CDS             complement(177..1772)
                     /locus_tag="VBC23_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6843513.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
     gene            2157..3227
                     /gene="xerS"
                     /locus_tag="VBC23_001578"
     CDS             2157..3227
                     /gene="xerS"
                     /locus_tag="VBC23_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6843514.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            complement(3270..4208)
                     /locus_tag="VBC23_001579"
     CDS             complement(3270..4208)
                     /locus_tag="VBC23_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6843515.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(4205..4873)
                     /locus_tag="VBC23_001580"
     CDS             complement(4205..4873)
                     /locus_tag="VBC23_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6843516.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(4983..6938)
                     /locus_tag="VBC23_001581"
     CDS             complement(4983..6938)
                     /locus_tag="VBC23_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843517.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(6940..7692)
                     /locus_tag="VBC23_001582"
     CDS             complement(6940..7692)
                     /locus_tag="VBC23_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843518.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(7718..8680)
                     /locus_tag="VBC23_001583"
     CDS             complement(7718..8680)
                     /locus_tag="VBC23_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO6843519.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            8846..9334
                     /locus_tag="VBC23_001584"
     CDS             8846..9334
                     /locus_tag="VBC23_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEO6843520.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKTLLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(9366..10214)
                     /locus_tag="VBC23_001585"
     CDS             complement(9366..10214)
                     /locus_tag="VBC23_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6843521.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(10347..11048)
                     /locus_tag="VBC23_001586"
     CDS             complement(10347..11048)
                     /locus_tag="VBC23_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843522.1"
                     /translation="METNRYKHFKNIITSERYFSNYISYKIMPTVSEFLQDMIYLADE
                     QKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSLNHLKLFEKEFDDIT
                     FTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLNNNAKVYLAIKDNQIIGD
                     VTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILISEEENRKMYEHQGY
                     EEVAYYWTALRSPRK"
BASE COUNT         3647 a         1912 c         1682 g         3826 t
ORIGIN      
        1 gctataggtc ttgtttttac tgcaaaatcc attgcacgtt acgataaaat ttctaaaagt
       61 caggtttttg ccgaatatta tttaattgga tcattgttct caattataag cgttttgata
      121 actcattggt tacttttggt atagaaaagc gctagaaata tcctaacgct tttcttttat
      181 tctgtcttat aagcataagt aaaatcaaca ttcaaaccag ttgaatttct taccaacccc
      241 tttaatttag aattttggag tccttttttc cctaggtaat aaactgggtt ataatatgat
      301 tgatcaaata aagctttttc agcagttttg taatcattag cggcatctcc tggtttcaag
      361 gcgtcagtag taattgcctt ttgataggca ttgtcatact ctttagaaga aaatttacca
      421 taattatacg cagaattagt cgtgaataac ccatagaaag ttgatccttc tggataatct
      481 cctccccatg agaagagcac gacatcaaaa ttttcatttt ttgcatcttc aaggcgttgc
      541 ttgaaagtaa caaatttttc ttcaacagtt agaccaggca aagcactctc ccatgtagat
      601 ttcacatagt caacagcatt tttagctgct ggagagtccg aatcagctgt gattgttaac
      661 ttgagagatg actgaccaac ctcagcaaga ccttctttaa aaagtttttg agctttactt
      721 gtattatacg tataacctgg ggcagtatac ttggaaagat cctcaccatt aggtaatttg
      781 gctaattttt tagggaccag agattcagca ggagttgaac ctgtatcaac tgccgcttta
      841 acaacacctt tacgatcagt cgctaaattc aaagcttgcc taattttctg atttgtcaat
      901 gctttaaccg aaccagtttg gttataaata gcagaagttc ctgatatttc agcaaaatca
      961 atctgacctt gcttgtacat ttgaactgca gtatctggtt ttttgatagt ttgaactatg
     1021 acactatttg ttttaacatg tttagaatcc caataatatt tatttttgac taatttaaat
     1081 ttaccatttg aaccattcca atccttaaca aggtaaggac ctgagtaaat ttgatttttt
     1141 gatgttgtac cataatcttt accaactttt tcaacatacg attgtttttg aggcataaaa
     1201 ttagagaagg ctaaataata tttaaattga gggcatggac ttgtcaattt aaaggtcact
     1261 tgattgcctt tagctgttac accaagttta tttaaatctt taatatctcc agaattaatc
     1321 ttatcagcat ttaaaaggtg cgattctgtc gccaagtaag catattcaga tgcagtttta
     1381 ggatcaacta ttctccgcca tgtgtaaaca aaatcttcag cgcttagttt gcttccatct
     1441 gaccatttta aattatcacg aagtgtagca gtataagtaa gaccatcaga agaaacggat
     1501 acttttttag ctaaatcggg ttttggtttg ccctctttat caattctaag taaattgctg
     1561 cctgaattac ctatagctaa gtttgaataa gcatctgtat tctttgaaat gtccaatgta
     1621 ctgatttcag taggaatata ccaattaata actttattat ctccagagtt ctttgactca
     1681 ttaccacagg caaccagggt aactgtggtg gctgttagag tcgccaaaga tatacccaaa
     1741 aacttaagtt tttttctatt aaaactagac ataataccag tcctcctaaa ccttttttta
     1801 taagacatag tagatctaat attgagaaaa ctagtatatt ttaaagtaga atttaacttt
     1861 ggttattaac ttgcaaatat aattaaaaat aattgagtac actttattac cttttcagat
     1921 accgataaaa tcaatgaaat tatgaatcag ttttctcaaa atactctgtc tttactataa
     1981 caaacatgtt aaacgataat aagtaatact gatcatcata aagaatggat atttgatgta
     2041 cttaccgaat gaattcctgt ttttaacttt taaactttcc gaaaaactat aattttcttg
     2101 aaagttatga tagagtatga tatacttcta tttatagata taatggagat tttattatga
     2161 aacgtgaatt actactcgaa aaaatcgatg aactaaaaga aattatgcct tggtatgttc
     2221 tagaatatta tcaatcaaag ctttctgttc cttatagttt cacaacactc tacgaatatt
     2281 taaaagaata tagacgtttt cttgaatggt tattggattc tggtgttgct aattgtcatc
     2341 acattgcaga aattgagctt tcagttttag aaaaccttac taaaaaagat atggaagctt
     2401 ttatactata tttacgagaa cgccctttat taaatgctaa cacaaggcaa aatggggtgt
     2461 ctcaaaccac cattaacaga actctatctg ccttatctag tcttttcaaa taccttactg
     2521 aagaggttga gaatgctgac ggggagcctt acttttatcg caatgtcatg aaaaaagtct
     2581 caaccaaaaa gaaaaaggaa actttagctt ctcgggcaga aaatattaaa caaaagttat
     2641 ttttgggcaa tgaaacgata gaattcttag agtatataga ttgtgaatat caaaacaaac
     2701 tttccaaacg tgctttagct tttttcaaca aaaataaaga gagagattta gctattattg
     2761 ctttgctact agcttcagga gtgcgtctat ctgaggcagt aaacttagat ttaaaggata
     2821 ttaaccttaa tgtaatggtt attgatgtca cacgaaaagg ggggaaaagg gactctgtca
     2881 atgtcgctag ctttgcgaag ccctaccttg ctaattattt agacatccgt aaaaatagat
     2941 acaaagcaga aaatcaggac attgctctat ttttatcaga ataccgtggg gtgccaaatc
     3001 gtattgattc ttcaagtgta gagaaaatgg ttgctaagta ttcccaggat tttaaggttc
     3061 gtgtaacacc tcataaacta aggcatactc ttgctactag gctatatgat gctacaaaat
     3121 ctcaagtatt agttagccac caactaggtc atgcttccac tcaagttact gacctatata
     3181 cccatattgt taacgatgaa caaaagaacg ctttagataa actatagtaa cataaataaa
     3241 gttatgtaaa tattttattt tacataactt tatttatgta tagaaacagc agtcccataa
     3301 ttaattttgc tatccaccga aatggacaaa ttcagactag ataagacttc ttttgtcata
     3361 tataacccca tgcctgttgc cttttggtgc tcatgaccgt tgtaacccgt aaagccatct
     3421 tcaaaaagtc tagggatatc ctcttcgagt atgccgatac cgtagtcttg tattctaata
     3481 ctctcttctc ctatgcttat tattatcttt gactcaggat tagaatattt tatggcatta
     3541 tctagcacct gtgaaagagc aaaagttaac cactttttgt ctgttttcca gatattatcg
     3601 ccttcaatta tgatagataa gcttttggat agacaaataa ccttatacga ttttataatt
     3661 tctactacta cttctctaag gctaacagct tcaaaacgaa aatcgtcacg atattgtcta
     3721 aatttcaaaa atgctaacaa cgtttcaaga taatgttgca atttcaataa ttgttgttca
     3781 acttccttag gatcgagatg atttgtctgg gccattaatg aaatagctgc caatggaact
     3841 ttcatttggt gcgaccacat ctttatcata agtgcaacat gattggtttg ttcaattgtt
     3901 tcagaaactt tttgcgcttc tctagcttct aattgtgtaa taatattaaa ataagcgtaa
     3961 tcactcggtt gaagttcgaa agaggggtcc tgactactat taaaatattt gagatgtaac
     4021 atttttttcc tgtaacgact gtattgccaa atactaattc ctagtaaaac aataacattt
     4081 aaacctaagg aattaaacaa atagggcatt ggtaaatgat atagatagaa actaataaaa
     4141 aataggacaa acatcatgat atataaaata taccaaatca agtgttctct taaaaactgg
     4201 cgtatcattg tagtaaatac ccaactcctc tcactgtatg aatgtaatca aaacctattg
     4261 ggacaatttt tttacgtaag cgtgtcatat taacattaag tgtattttga tcaataaaac
     4321 tatcattttc ccaaagtttc tctaatagag actcttttga gactacttgt ttaggatgca
     4381 tgagcaagat agataggatt ttattttctg ttggcgataa aataacctct ttatcttggc
     4441 tagacaataa cccttctctt gtcaacgtaa atcccccaaa agttaattcc tgttggataa
     4501 attgttgact tctccttaaa atagcagtta gcttagcatc taatacagct agagagaatg
     4561 gttttgaaat aaagtcatca ccccccatat ttaatgccat aaccatatcc atttcatcat
     4621 tactagatga aatgaaaata ataggaattg ttaaaaactt acgcaattct gcagtccagt
     4681 aaaaaccatt aaaatagggt aacgtaatat ccattagtat caaatcgggt tgaaatgcga
     4741 taatttcttg tttcacatca cgaaaattgc tgacactaga gacatgatag ctagctgata
     4801 aatgatcttt taaaagtgac acaatcgtca tatcatcttc tacaatataa atttttcctt
     4861 gctcttgtga catatcccct cctaaaatag tacgaaatct ctttctttca tttagtataa
     4921 caaatttttt agtctatcgg tttctattaa tgcaaaagga ttggtaaaat aaccaatcct
     4981 ttttagcgtt caataatatg ataataagtt cgactagtta tacggtaaat gataaagtaa
     5041 attatagaaa tcgcaagtac tgtcaaacca cttactacat atacgattgt tgagttaagc
     5101 accccaaata ccaaaagcat ctgttttagc attggaatag caactccaaa gtgaataact
     5161 gctaccacta aaggttggaa gaagaaaatc ataatttgag aattaatagt ccgtttaact
     5221 aattttttgc tcatacctac ttcttgtaag atacgataag atctcttatc ttcatgacct
     5281 tctgagtatt gcttatagta aacaattaga gctataccta gcaaaaagct tatacctaac
     5341 agaaatccag taaataataa cccaccaaac atttgataac cttcttttag aaaactctgt
     5401 ttagtgctaa tatttccaac atacttacca tctacctcta ttatgtcgtt tttagaaatt
     5461 gaatttatct cttgactatt caaatccaaa tatgccttaa aggtttttgg aaaagtattg
     5521 atgttcttag tataaggtaa atatgctttt ctaatattgt caatttgttt gttgtttgca
     5581 aatataatca aacccgggtt gtaagtattt gtagtctctg ggacataagc ctcttttaaa
     5641 ttttttacaa cgtcaaattt atttcccaat agatttattt ttttcaaacg gctatcacct
     5701 ttttgaacaa agtaagccac ttggttatct tttaattttg gtaattggtg acctaaacga
     5761 cggaaatcat tctgagtaat taggtacata aaacccgttt ttgtgatatc aacatgtttg
     5821 acgtttttag atgtgatatt aagttcactt gattgactaa ctggcatcga aatcattgtc
     5881 tgattataag tgatagcttc cttagatgac ttacctagtt tcttcaaaat cttttcttca
     5941 aatatatttt tcgcgtcacc ttttgaatta tctattgata aacttactga ttttggaaat
     6001 agtccggtaa caacattttg tgtatttgaa tagagtgaga ctgttgtagc aatagtaact
     6061 agagccataa cagctaataa agtgatactt gctaacccta ctgcattttg cttcattcga
     6121 aaaatcattt gcgaagttga tacaaaatgc tcagatttat aataataatg cttgttttga
     6181 cgcaaccttt ttaagtacca tgtcataaaa ctaatataaa aaagataagt accagcaata
     6241 actaaaagta ctgcaaagaa gaaacgatag ataacagcta atgcaggtgc tttacctgaa
     6301 gtaagagcca ttgtataagc gatggcgata gctactaacg ctaaaattgc aagtataaga
     6361 ttaccttttg gttctttttc tccctgttgt ttctttctaa aaagacttaa tggggatgat
     6421 aaatgaacat gtcgaataac tggaacttct aaagtcagaa aaatacctgt aaatataacg
     6481 atacaaataa taaatggcca taaacttaaa ctaagattta gtgcatgata gttaataatg
     6541 ttgacaaaaa ttaaataaat aaatttagca aaaaaagcac taaacagact tcctatagaa
     6601 ataagaaata tataaataat aaacagctct agactagcta cacgcgcaac ttgacgttta
     6661 ttcatcccca aaatgttata cagtccaaat tcactactac gctgtttcat caagatattg
     6721 tagctataat gttccatgac taccgcaaag attgacaaaa caatcactcc aagaaaaaga
     6781 accgttgccg ctgtccccat atctcttcca acagcactca ttaagattag tagcgtagaa
     6841 caagtcaatg aataaagtaa taaactggct aagaggaatg gtatgtactg gtctatagaa
     6901 tgttttaaat tatgccaggc tattttgaga taaaacatat tattcacccc ctaatatggc
     6961 agacattgtt aaagaaatat ctttattgaa ttcgctatta ttcttattac cacgataaag
     7021 ttggtgaaaa atacgtccat ctttgataaa taaaacgcgt ttggcatgac tagcagcatt
     7081 tgctgaatgg gttaccatca aaatagtttg tccatccaag ttaatagttt caaataaatt
     7141 cagcaagtct tctgaattac ggtaatctaa cgctgctgtt ggttcatctg ctaataaaat
     7201 ttgagggttg gttatcaaac tacgagcaat cgctacacgt tgcttttgcc caccagagag
     7261 ctcgaaaggt ctcttatcta ataagtcatc aattctcaga tgagaagata attctgacaa
     7321 acgatgatcc atttctttgt aacgtttacg atcaagaact aaaggaagat agatattatc
     7381 tcttacagag agagtatcca aaaggttgaa gtcctgaaag acgaaaccaa gatttttcaa
     7441 acgaaaactc gctaattttg cctctttaat tttcgtaata tcttccccat ttaaaatcac
     7501 ttgtccattt gtcggttttt ctaaagtagc taaaatattt agcagagttg tttttcccga
     7561 accagactct cccataatag caatgaattc cccttgttca actttaaaat cgacgtcttg
     7621 tagagccctt gtctcttctt ttgaaaaacg tgtacgaaaa actttttcta agtgattgat
     7681 ttctaataac atataattct cctttattta ttatacccta attttgattg attctactct
     7741 ttaaccaaga ctgtgactct tgtaaaggaa ggctggttac ttgatctggt aagatagggg
     7801 tgttctcgta tttatatcct tgtcgatcac taactattcc tgtagttaaa gctaatctag
     7861 caccgtcgta aagcatgaaa gtttcattaa cagtcgtata tcctgctgta gcttgaccaa
     7921 agcttttaac attaggtaat cctttaaatg ataaaaaagt catttctgcc gaactagcag
     7981 ttttatgatt agttataata gcgattggaa catgcttagt attgattgtt ttcctagata
     8041 tttttaatgc ttctagcggt atatttttca tcgtgattgt tttcttattt ccatacttat
     8101 cagtataatg aaatagggta tcatttggta aaatagaggc taacccccca atcataggga
     8161 tcatattgcc gccagtatta ttcgataagt ctaaaatgag tcccttataa cttttttcca
     8221 tcaatatatt ttgtaattta ccactataat ggctaataga tcgtttatct aacttactta
     8281 tagaaggaat agtaattaag agaatgtctt cgtttaatct tcgatattct ggatatcgac
     8341 tatctagggt atctcttcgt acagtctctt tggataatat ttgactatgt ttaccgcctc
     8401 cataccgtac tgcttcctgt acaatgttgc gactctcttg ataagatttt gacgttttca
     8461 actttttttc tgatcgttgt cttataattt gccattgacg accttgcgaa tacaaaccac
     8521 gctgttcaac acgatctaaa ataacacgtc cataacgttc gctactagga ggtaatagat
     8581 aaatatttaa tgctgaacca taataatgaa ctaccactcc taaaattcct aaaactataa
     8641 gcattggaac tacaaaaagt aaaactattt ttcgccgcat ctcttgctcc ccctttctct
     8701 ttaagtctat tatatggcaa aatggtttac tataacctta catcttaaga cctatttctt
     8761 acaaatttga aagttttaaa agctttataa actataattt tgatataata tcttacataa
     8821 caaatggaaa gtacggtgaa aacatatggc caagacaagt aaaaaattga caaaatcttt
     8881 agggcctatt ggtaaattaa tttcaataat ccctgacaca actgaactta ttggaaaagc
     8941 aattgataat tctcgtccaa ttattgaaaa agaacttgac cgtcgtcatg aaaaaaaaca
     9001 gacttatact cagcttgata atgtagtaca ccttgatatc aatgatgcgc aagtttactt
     9061 agagcaacgc cattttaatg tatcaaggat tgttgctgaa ccaaattcaa agtgggcaac
     9121 cctacatcct aatcaagttg tgaatatgtc gcctaaagct ggtagggtca gaatcggttc
     9181 attagtgaag ttgtactata ttacagagga tatccttgaa gaaagtaaga cactactttt
     9241 atctaaatcc gaaaaaagta aagcacgaca acaacggtta acagaagtat tgagtacccc
     9301 tagtcaatta ctcaaaaaaa atcgaaataa ataagcatga tcgaattgac catgcttatt
     9361 tatttttacc aaacaggttg atctaaacct tgagatgttt tttcgataac ttttttaacc
     9421 gcatctgtat ggtaagcttt cacaacttct ttaatggctt tagcttttga tttgtcttgc
     9481 caaccttttt gagcaactaa aaggttgtac cattgttttg catttttact ttttggttca
     9541 ataaaaatag ctgatttagg atttattcct gcttctgtca caaaatcatt attaataact
     9601 gctgcatctg tggaatccaa agaacgtgcg gtctgtgctg catctacttc ttttaaatct
     9661 aaagatttag gattggaaac aacatctgac attgttgcta atgtatcacc tgatactttc
     9721 aattttagca agcctgctga ttgtaacaag tagagagagc gactttcgtt aactgcatcg
     9781 tttgggatag taatagttgc tttatttgga atttcagaaa ctgtttggta tttaccttta
     9841 ccgttcttag taccagagta taatctaaag gaagtaaagt atgtctcagc aacggaaaca
     9901 agattggtct tatttgcttt attccaattg tttaagtaat tataatgctg gaaagcatta
     9961 atatcaatct catcactttc taaagcttta ttaggttgtg tataatctgt gaattccgta
    10021 aattttagct taacgccttt tttcttaact aattcttcga ttttatccca acgagctttt
    10081 tcagttttac tctttaccat tgttccaact ctaacgactt tggcgtcttc atttttagac
    10141 tgaccacagg ctcctaaaat aagtgctgaa attactactg ttgtcgttaa ccctaatagc
    10201 tttttgattt tcataattta tttctccttt gatatggttg tatgattaat caactgatac
    10261 tagtgtaaca aaaagctaat ggattaaaaa atacttatta tcaatcaaga gatatagctt
    10321 ttaactatat catttctttt ataattttac tttcgagggc tccgtaaagc ggtccaataa
    10381 taggcgactt cctcataacc ttggtgctca tacatttttc tgttttcttc ttctgaaatt
    10441 aagataactt tcttaaaccc cctacttgca tcaaattgta ggcgcgtacc aattccttgt
    10501 cctctgtagg actcttggac ataaaaatca tctatttcca cgtattcacc atagtcccaa
    10561 gctgttacat ctccaataat ttgattatct ttaatagcta ggtaaacttt agcattatta
    10621 tttaaaagat taacacgatt atcattatac atttgcttag cgtaaatttc tccgtaaact
    10681 atactttgtt tatatttgaa ctttaggtat ttttctaggt aattttcatt aagtttagta
    10741 aatgtgatgt catcaaattc tttttcaaaa agtttaagat gatttaatga atttgtaaat
    10801 atgatatgtt tctctgattc aaagccttgt tgtgataaag ttctctgtaa ttcactgctc
    10861 aattcagcat tttcctcaaa aaatacaaaa gcaaaatcag actgataatc tttttgttca
    10921 tctgctaaat agatcatatc ctgtaaaaac tcacttactg taggcattat tttgtagcta
    10981 atatagtttg aaaaataacg ctctgaagtg attatattct taaaatgttt ataccgatta
    11041 gtttccaaat aagctttttc ggatgac
//
LOCUS       DATRME010000062        11053 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRME010000062 DATRME010000000
VERSION     DATRME010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            707..1831
                     /gene="mutY"
                     /locus_tag="VBC23_001587"
     CDS             707..1831
                     /gene="mutY"
                     /locus_tag="VBC23_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6843523.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            2008..2601
                     /locus_tag="VBC23_001588"
     CDS             2008..2601
                     /locus_tag="VBC23_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843524.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2647..2961)
                     /gene="trxA"
                     /locus_tag="VBC23_001589"
     CDS             complement(2647..2961)
                     /gene="trxA"
                     /locus_tag="VBC23_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6843525.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(3042..3542)
                     /locus_tag="VBC23_001590"
     CDS             complement(3042..3542)
                     /locus_tag="VBC23_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6843526.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(3546..5885)
                     /locus_tag="VBC23_001591"
     CDS             complement(3546..5885)
                     /locus_tag="VBC23_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6843527.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(5970..6512)
                     /locus_tag="VBC23_001592"
     CDS             complement(5970..6512)
                     /locus_tag="VBC23_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6843528.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(6515..6826)
                     /locus_tag="VBC23_001593"
     CDS             complement(6515..6826)
                     /locus_tag="VBC23_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843529.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            6937..7830
                     /locus_tag="VBC23_001594"
     CDS             6937..7830
                     /locus_tag="VBC23_001594"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6843530.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            7846..8439
                     /gene="lepB"
                     /locus_tag="VBC23_001595"
     CDS             7846..8439
                     /gene="lepB"
                     /locus_tag="VBC23_001595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6843531.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            8568..10988
                     /locus_tag="VBC23_001596"
     CDS             8568..10988
                     /locus_tag="VBC23_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6843532.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELSGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
BASE COUNT         3734 a         2075 c         1776 g         3468 t
ORIGIN      
        1 atgttttatt acctttggta acaccaaaac atacacgaac ttttattcat atgtgttacc
       61 attttacatc tttcatattg agatatttga ttagcaatac ctccttgact aacaaaaatt
      121 actaacacat ctaggggtat accctagttt atatttgttc atgattacct caagcgagca
      181 tgcgctaatg gtaatgaact attaccacat tgtctgtctt ccttaaatta tataaggact
      241 tatgtgtcac cgttactgta gcgacctaca tctaaaccta acataagtgc ccctccaact
      301 atttctagga aatgaagaaa acttcaaatc cttgtgactg tttgcaacat caatgcattg
      361 ataacaattt tagtgtatcg ccattcttat agcgacccat attaaaaatt aacataagcg
      421 ctacctccat taactaaagt tagcttgcct accaatcttt cttttggaga ctagtctcca
      481 ttcttatacc tagagatatt gtaatagatc atggataacc tccttgattt aaactttatc
      541 tttataattt aattatagtg taaacgcttc cttaagtcaa gaaaaaaata ttgatgcaac
      601 atttttcttc atcattaagt acgatctagt caacatacct ctttgtcaaa tcctctttct
      661 ttgatacaat taatctatga tagatttaaa agactatgga attgagatgt ggcctgaaga
      721 tagaatcgct tcttttcgtc gcacactttt agggtggtat gaccaagaaa aacgtgatct
      781 cccttggcga agaactacta atccatacta tatttgggtt tctgaaatca tgttgcaaca
      841 aacacaagtt aatacggtaa tcccttatta taaacgtttt ctagagtggt ttcctcaaat
      901 aaaagatttg gcagacgctc ctgaagaaca attactaaag gcatgggagg gactaggcta
      961 ttattctcgg gtgcgtaata tgcaaaaggc tgcacagcaa gtgatggttg attttggtgg
     1021 catatttcct cacacttatg atgatattgc ttcattaaaa gggattggtc cttatactgc
     1081 aggtgctatc gccagcatct cattcaactt accagagcct gctgttgatg gcaatgtgat
     1141 gcgtgtcatg gctcgtttgt ttgaagttaa ttatgacatc ggtgacccta aaaatcgtaa
     1201 aatttttcaa gctattatgg aaatactaat tgaccctgac agacccggtg attttaacca
     1261 agcacttatg gatctgggta cagacatcga atccgctaag acacctagac ctgatgaaag
     1321 ccctattcgt ttctttaatg ctgcttattt gaatggtact tatagtaaat atcccataaa
     1381 aaatcctaag aaaaagccta aaccaatgcg aattcaggct tttgtcattc gtaaccaaaa
     1441 tggtcaatat ctactggaga aaaatactaa agggagactt ctagggggtt tttggtcatt
     1501 tcctattatc gaaacgtcac ctctttctca acaattagat ttatttgatg acaatcaatc
     1561 caaccccata atctggcaaa cccaaaatga aactttccaa agagaatatc aattaaagcc
     1621 ccaatggact gataatcact ttccaaatat taagcacact tttagtcacc agaagtggac
     1681 aatagaactc atagaggggg tggtcaaagc aacagattta cctaatgctc ctcacctaaa
     1741 gtgggtagca attgaggact tttctctgta tccctttgca acaccacaaa agaaaatgct
     1801 agagacctac ttaaaacaga aaaatgctta atcacaagtg aactatgacg gttcactttt
     1861 tgtttacaat tcctttacgc cgctgtaaag ttttcctcag attatctttt aataccctat
     1921 aaatagtggt ctatccctaa tgtcaaatac atttgataga cccgttagct tatcatcctt
     1981 atactaatat tatgaaagga gatatttatg gaaattggtc agcaaattat tcgctaccgt
     2041 aaacaacaag cgctctcgca agaagaatta gccgagaaag tctatgtaag ccgtcagagc
     2101 atatctaact gggaaaatga caaaacttac ccagacatcc atagcctctt actcctaagt
     2161 caaatttttc aagtgagctt agaccaacta attaaaggag acattgagaa aatgaaatat
     2221 accatcactc aggttgataa aaagaatttt gaacgagata ccaaagtaat ggtgacattg
     2281 atgatactat taatgatttc aagttatcca ttagtctatt tcttagagtg gctaggactt
     2341 ggcatttttg ttttattatc gataattact atgacttatg ctaatcgtgt ggaacgtttt
     2401 aagaaaaaat atgatgtgca aacttataag gaaatcctag ctgtttcaag tggaaaatta
     2461 ttagacgaaa tcgaaaaacg ggaagaaaga gcaaaacttc cttatcagaa accccttatt
     2521 gtcactgtat ttttcctcat tactgttgcc accttttttg caagtcgttt tattttcaca
     2581 tggttgttcc atttaagtta gtagataata aaataaagct cagcgtgaca ctgggcttta
     2641 ttttatttaa ctcaattcag caataatagc tttcagttgg tctttagtat gaacacctgc
     2701 aacttgttta accacttctc cgtctttttt aaacatcaat gttggaattg acataatacc
     2761 aaactgacgg gcagtttccg gattctcatc aacatccatt ttcaaaattt tgagttcatc
     2821 ttcatcgatt tcttgagata actgttctaa gattggtgct tgcatacgac atggaccaca
     2881 ccaagttgcc caaaaatcaa tgagtactaa accttcttta gtttcttcta caaatgtagc
     2941 atctgttact tctaatgcca tcttttttct ccatttcatc ttaatgataa gcctattgta
     3001 caagaattta gttgtttttg aaactatttg ctacgaactc gctaaataaa taaacaaagc
     3061 cctaaaatca agccaataag gtaacctaca ataacatcct taggatagtg aatacctgct
     3121 atcacacggc aaattgccaa tagaaccgat aatattaaac aaactattcc aaagtaaaca
     3181 taatatcgta acaaacacat actaatcatc gtagctgaaa aaacatgtct gctaggcatt
     3241 gaccttccct cagtatcctt ataaattaaa ggtttaatat tccatttttc atatggtcgt
     3301 ggaaaatcaa ggcgtttcct aatgtaagac aaagctataa agccaacagc agggataatt
     3361 aatagcctta accataattg aaatgtcatc ccttgccaaa gagtaaatat cagaaaagat
     3421 ggatagagta tgtacatcat tcctgtgact atttttagta aaaaatttaa aagaattatt
     3481 aactgtgggg attttctaaa aggttgagat agtttggcat aaaattgttc atatgattcc
     3541 ataatttacc ctaacgttac aattgtggcg ccagatcccc ctgcattttg tggggcataa
     3601 gcaaaatgct taacgtgctt attacgacga agatattttg tcactccctc acggataacg
     3661 cctgtaccaa taccatgaat gatatcaact tgtcccatat tgttaagcaa tgcttgatca
     3721 ataaaattat ctaactcttg catagcttct tcgtatcttt tacctctaag atcaagtcga
     3781 gctcttggtc cagaactatc agccttttta accacattaa tctgtttact tttgactttc
     3841 tgttcttctt ggactctaac aagggtaaat tcatcttgtg ttaatgtcat tttgataatt
     3901 cccacttgtg cttcccaacg tccatctttt aattgactag ttaaggtacc tcgctgtcca
     3961 tagctagtca ctataatatc atcaccaatt ctaggagcac gagctgcttt aattttttta
     4021 gccttattta agactttatt ttttgataaa tcaacttgag gtgctaattt ttttatttgt
     4081 gccttagcat ctataatttc gtgaggtttt aattggctct tatcattgag tttttttaag
     4141 atagtatcac tctcattcaa agccatatct acaatttctt gagcttcttg atagattttt
     4201 tctaactctt tatcgcgctc atgtgaaaat tcattataga gtttcttaac cgcacgattg
     4261 aatttgaggt tttcttgttc aacttcttta atatgatcca gtcttctacg tgtctcaagt
     4321 gtctgtgcct ctaactgttc aataatacgg ttaacatctg agtcagaatc tgtcatctgc
     4381 ttagcttgtt taacaataaa tggagctaaa ccaaggcgag aagcaatttc aaatgcattt
     4441 gatcgtccag gaactccttg cataaagcga tacgtagggc taagcgtttc ggcatcaaat
     4501 tccatgctcg cattctctac aaaatttgtc tcaatcccat aagcttttaa ttctggatag
     4561 tgcgtggtcg ccatcgtttt gatattactt aacctaaggt gttctaaaat agccatagcc
     4621 aaactagcac cttcttgagg atccgttcct gctcccagtt catcaaagag gactaaacta
     4681 ttgtggtcag cctcgtttaa aatactgact atgtgcgtca tatgactaga aaaagttgat
     4741 agactttgtt caatagattg ctcatcgcca atatctgcaa agatattgtt aaatactgca
     4801 attttactac ctttatccgc taatactggc aaaccagact gtcccattaa ttgtgctaaa
     4861 ccgagtgttt ttagcataat cgtcttacca ccagtattgg gaccagtgat gacaattgca
     4921 gttaaatctt gatcaaaatg taagtcatta gccacagggt tacttaacag aggatgacga
     4981 acattgatta atgctaacgt gctgtcatta gaaatctcag gtatcgtcgc cttattatca
     5041 gacataaaaa gatatttagc ccttacaaaa tcaagatgcc caagaatcca tgcattattt
     5101 ctaatagtgg cgacatgggg tcttaacaag tctgaaaatg cgtgtaaaat acgactttct
     5161 tcatgacgtt cgtcagctct aagctgcgtt atctcttcgt ttagtgtaac tacagcacga
     5221 ggctcaatat aaacagtact tcctgaagaa gagatgtcat gaaccacacc agaaatacga
     5281 ttccgataag tattttttac tggtaggaca cttcgtccac tacgactagc gattagattc
     5341 tctgataaaa gctctgcttt ttccttaagc atatcctgta aaatctgacg aacccgtcgt
     5401 tcactgtttg ttaattgacg acggatacgc tctaattctg gactcgcaaa atgttctaaa
     5461 aaaccaccat cattgatagc ttgaaaagac ccttgcagat tagggaattg ttccaaattt
     5521 tcaaacaaac gatccaaaga ttggaaagaa acattatcca aatcagaata aaagtgaatc
     5581 atatccgaag aactctgtaa aacttttttg atagccaaaa gttcttgaat attaagatca
     5641 gctgaaagct ctaagcgttt taaactctca gagattgaac ttaggctgac tatgccaaat
     5701 gagtgatgtt caacaaaaat ctgttccatg tcagaaattt cattaaaact tttttctatt
     5761 ttaggagcct ctgtcatcgg ctccagctct gataattctt cttgtgattg ttctgtcttg
     5821 agataaggta atatcaattc cttaactttg ttaaattcta actgttctaa aatcttgtta
     5881 ttcatactct tcattatacc taaatttatc acaaaatgac aacgttaaaa aggttggaac
     5941 tcttactcca accttactaa acattatatt caaagaccta ccgcccaagt ccttaccaaa
     6001 aactgtgaga agaatggtaa gtcgttaatt aaaaatctaa caaacagact attccataac
     6061 ttttcttgaa caaatgacat agggactgtt gccagaatag tcaaacacat actcataaat
     6121 aagagacaaa ccagaacggc taaaaaacca ctaataatat tattttgcaa gctatcaaaa
     6181 tgttcaactg gtgctaaatg tagtaaaaca cctaacaagc gacctactaa gtaagataaa
     6241 ctgtagataa aaacaaaggc aacacctgca taataaacat gactcaattg aaatttgcta
     6301 atatctttaa aaaagtaaac ttctactcct tgtacaggat tagaatacgg tacccataac
     6361 gtaatctgac tagctaattc ttggtaaaat tgactagcaa tcatcaaaga taccattgac
     6421 attaacacgt aaaagacttg taaaaaaata cctctactgt agccaatata aaaatgccaa
     6481 ataacaataa tcagcaaaag caatgatagc atgattaatc ctcactcact ttttctttaa
     6541 tgtccgaaat cgttttggag cgtaacttat tgagctcatc ttccattttt tcaatgtcaa
     6601 tttctcgact tagttgcact gataaagcat taattgccat gagaatagca atcgtttcat
     6661 tatcagcatt aggtaatttt tctttaatag cttcatactt ttctgtagct acacgctcaa
     6721 tctcttccat aaaaaggttg tctttatctg tcgtcaatgt gagaggctta tctccaaaga
     6781 caaatttata acgatttttc tgactattat ttatttttgt ttccatacta tacctctgct
     6841 agcattatac cgtaaaatag ggctttcgta aaattctacc ccttatcatt acttgtctat
     6901 tcccttattc ctctttttta tgatagaata ttgtctatga acacaatcgt catgcaagca
     6961 gataaaaaat tgcaagaaaa gataagaacc gatttagctc aacatcatat aagtaataac
     7021 aatccttatg tggtattttc agctaaaata tcaggagcta ctgttctttt atatacttct
     7081 ggaaaacttg tcttccaggg ttctaacgct tcacatatcg cccaaaagta tggttttatt
     7141 gagcaaaaag aaagttgttc ctctgagagt caagatattc ccataattgg tactgatgaa
     7201 gtgggaaatg gctcttactt tggtggactg gcagtggtag ctagttttgt aactcctaag
     7261 gaccatgctt acctcaaaaa attaggtgtt ggtgattcaa aaacacttac tgatcaaaaa
     7321 attaagcaaa tcgcgcctct tttggaaaaa gcaattccac acaaagcatt attgttatcg
     7381 cctcaaaaat ataatcaagt ggttagtcca aataacaaac ataatgctgt ctccgtaaaa
     7441 gttgctttac acaatcaagc tatcttcctt cttcttcaag atggttttga accagaaaaa
     7501 atcgttattg atgcttttac tagctccaaa aactatcaaa attatttgaa aaatgaaaag
     7561 aatcaattta aacaaacgat tactctggaa gaaaaagctg aaaataaata tctagctgta
     7621 gcggtcagtt ctattattgc acgtaatcta ttcttagaaa accttaacaa attaagcgac
     7681 gacgtaggat ataaacttcc aagtggagct ggtcaccaat ctgataaggt cgctagccaa
     7741 ttactcaaag cttatggtat ctcttcgctt gaacactgcg caaaacttca ttttgccaac
     7801 accaaaaaag cacaagcatt attaaaataa atagcaggga aaattatgaa agaatttatt
     7861 aaagaatggg gtgtctttat cctcatcctc tcactttttt tactatcgcg tatcttttta
     7921 tggcaattcg ttaaagttga cggacactcc atggatccaa ctttagctga caaggaacag
     7981 ctagtagttc tcaaacaaac aaaaatcaat cgattcgata ttgtagtggc taacgaagaa
     8041 gaaggcggcc aaaagaaaaa aattgttaaa cgtgtcattg gtatgccagg tgatgtcatc
     8101 aaatataaaa atgacacctt aactattaac aataaaaaaa cagaagaacc ttacctcaag
     8161 gaatatacta aattatttaa aaaggataaa ttacaggaaa aatattcgta taacccactt
     8221 ttccaagacc tagcacaaag ctctaccgct ttcaccactg acagcaatgg cagcagcgaa
     8281 tttactactg tcgtgcctaa aggccactac tatcttgttg gtgatgaccg aattgtctct
     8341 aaagatagtc gtgccgtcgg ttccttcaaa aaatcaacga ttgtgggaga ggttaaattc
     8401 cgcttctggc caattcgtcg ttttggaact atcaactaaa ggaaattaaa agttcacctg
     8461 acaaaagttg aaatagcata ttatattgaa aatagggagt gacaaatcca tttaaaatag
     8521 ggatttacaa tcactccctt taactgttta ttaaaaagga aggtacaatg gaagtatttt
     8581 ttacaggaac cattgaacgc attatatttg aaaatgccag caattttttt aaaatattac
     8641 ttctggaaat cgaagatacg gatagcgatt ttgatgatgt agaagtaatt attactggta
     8701 ctatggccga cgttatagag ggagaagaat atactttttg gggaacacta acccaacatc
     8761 caaaatacgg cgagcaacta caaagtgtac gctatgaaag agccaagcct acttcaggtg
     8821 gccttgtgaa atacttttct agcgaacaat ttaaaggaat tggtaaaaag actgcccaac
     8881 gcatcgtaga actctatgga gataatacca ttgataaaat ccttgaatcc cctgaacagt
     8941 taagtacaat ctcaggacta tcaaaaatta atagagaagc attcatcgct aaactgaaac
     9001 tcaattatgg aacagagcaa gttttagcta aactcgctga atatgggtta agcaatcgtg
     9061 ctgctattca aatatttgat cactataaag aagaatcact tgaagtgata aacgaaaatc
     9121 cttatcaact tgttgaggat atccaaggta tcggttttaa aatagctgac caacttgctg
     9181 aacaagtcgg tattgaaagt gattctccta aacgctttcg tgctgctatt attcatactt
     9241 tagttgagtc ttctatggag caaggagata cctatattga ggctcgtact ctcttggaaa
     9301 aaaccattac tctccttgaa gaagctcgtc aaatcgaact agatccttca attgttgcta
     9361 aggaattgac aaatttaatt gcagaagata aagttcaaca tattggaact aaaattttta
     9421 gtaatactct attttttgcc gaagaaggca tcaaaaaaaa cttacaacga atactaaatc
     9481 agcctcttga taaacaacta aatcacaaag atattgaccg tgaaatcaga gatattcaaa
     9541 aatctttaaa tatccactac gataatatcc aagaaaaagc tattagagaa gctttactaa
     9601 gtaaggtatt tatcttgact ggtggcccag gaacaggtaa aacaacagtt ataaatggta
     9661 ttattgaagc ctattccgag ttacatcata ttgatttaaa caaaaatgat attcctattg
     9721 tacttgctgc tcctacaggt cgtgctgcta gacgcatgaa tgaacttagc ggtttaccga
     9781 gtgctactat tcataggcat ttgggattaa acggagatag tgactatcaa agtctagatg
     9841 actatctaga ttgttcctta attatcattg atgaattttc aatggttgac acttggcttg
     9901 ctaatcaatt atttgacgct ttggactcgc acacacaagt tattattgtc ggagatagtg
     9961 atcaattacc gtctgtaggt ccaggacaag ttctagcaga tcttttaaat ataaatgcac
    10021 ttccacacgt taaacttgaa aagattttta gacaatcaga agaatcaact attgtgactt
    10081 tagctaatca aatgagacag ggctttttgc cagaggactt tacagctaaa aaagccgatc
    10141 gctcatactt tgaagcatct gctaatatca tcccaaatat gatttctaaa attgttcaat
    10201 ctgcacttaa gagcggtatt gaagctcatg aaattcaaat tttagctcct atgtatcgcg
    10261 gccaagcagg tattaataat cttaacctta tcatgcaaaa tctcctcaat ccattaaaag
    10321 ataataacca atttactttt aatgatataa atttccgaat aggagataaa gtgttacatt
    10381 tagtcaacga tacagagctt aatgttttta atggggatat tggttacata acggacctta
    10441 taccagccaa atatactgag agcaagcaag atgagattta tatgactttt gacggtcaag
    10501 aagttatcta tcaacgcaaa gaatggctta aaataacact agcctatgct atgagtattc
    10561 ataaatcaca gggttctgaa tttcaagttg ttattctgcc aataacgaga cagagtggtc
    10621 gaatgttaca gcgaaacctt atttatacag ctattacgag atctaaaagt aaactaatac
    10681 ttttaggaga aattggtgca tttgatttcg ctgttaaaaa cgagggggca aagcgcaata
    10741 cttatctgat agaaagattt gaaaacaagc aagaaatagc taattctcaa aaaattgaag
    10801 atagctcaat agatcaaaag attgacaaca caataattaa tacgtccatt ccaaaaacag
    10861 caacaccaat tgagcagact aatctttcaa aaataactta tcgcctaact gaagaaaatt
    10921 atttaacaat cgatccaatg attggtatta atcaacaaga tataagtgca atttttgata
    10981 gtaaataaat tttttcataa aatcttgaaa actcttccaa atatgttata atatcattgt
    11041 attaagaacg tag
//
LOCUS       DATRME010000063        10990 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRME010000063 DATRME010000000
VERSION     DATRME010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(81..767)
                     /locus_tag="VBC23_001597"
     CDS             complement(81..767)
                     /locus_tag="VBC23_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6843533.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVR
                     MRASFKTEES"
     gene            complement(896..2116)
                     /locus_tag="VBC23_001598"
     CDS             complement(896..2116)
                     /locus_tag="VBC23_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6843534.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(2303..3670)
                     /locus_tag="VBC23_001599"
     CDS             complement(2303..3670)
                     /locus_tag="VBC23_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6843535.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(3817..4863)
                     /locus_tag="VBC23_001600"
     CDS             complement(3817..4863)
                     /locus_tag="VBC23_001600"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6843536.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(5004..5600)
                     /gene="recR"
                     /locus_tag="VBC23_001601"
     CDS             complement(5004..5600)
                     /gene="recR"
                     /locus_tag="VBC23_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6843537.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(5615..7660)
                     /locus_tag="VBC23_001602"
     CDS             complement(5615..7660)
                     /locus_tag="VBC23_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6843538.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(7789..8481)
                     /locus_tag="VBC23_001603"
     CDS             complement(7789..8481)
                     /locus_tag="VBC23_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6843539.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(8658..9416)
                     /locus_tag="VBC23_001604"
     CDS             complement(8658..9416)
                     /locus_tag="VBC23_001604"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6843540.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(9597..10793)
                     /gene="tuf"
                     /locus_tag="VBC23_001605"
     CDS             complement(9597..10793)
                     /gene="tuf"
                     /locus_tag="VBC23_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6843541.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
BASE COUNT         3498 a         2163 c         1752 g         3577 t
ORIGIN      
        1 cataaaaata gctaaaaatg aaaaacatta tccagctatt tattttttaa tagctagata
       61 atataataat attaaaataa ttaagattct tctgttttaa aactagctct cattctcact
      121 ttttggaaaa tttgtttaac aaataggacg agaaaactta atagagttgt cactatcaaa
      181 aggtttaaaa agggatttgt tattcctagt attggtgtct tcatcataaa gccataattt
      241 cccccagtca agaggttaat aaaaatcaaa gacatattaa tgagaagcgt tagttgtata
      301 atcaattttg gatttaattt gtccaaattg gactgtgtca tcagatgtag taaactaagg
      361 acaaataaag caaaatgtcc aataaagaag aaaatattag tcacatgtag aaactggtaa
      421 gggtaaaagt ctgggtgaat cattgccaat actccaccta ctactcctaa caatgcgaaa
      481 taatccttta atattctagg cctcgctaag agaacgacaa acatgcctat tcgacaatgg
      541 taaaggggaa gactttcaga taaaggcaat tttgcccaga aaaaccaagt ataaatcgta
      601 attaactgaa gtcctaaaag gatactccaa atagttttat acaaaggctt atctctatta
      661 accaaagtca gccacatcag aaaaggtaag acagataaaa tcaaactatg gataagtaaa
      721 ggcatctgcg gaatctctgt tctgatatta gaaaagaatt ctatcatttt agtattatct
      781 ttctatcatt tttttactag atacttagta tatcaaatta tttgctataa gacaaaacta
      841 tttttcaaag aaaaaactct ttggtaaaac caaagaattt taaattagat atatactaca
      901 caactttagt tgttaatttt cttaaatata gagataacaa taatccgaat aagaataaaa
      961 aaccaaaaac cattaatgtc aattgatagg aatttcccca tgaatatgtc tttgataaca
     1021 ataggggccc aaacagacct gctattgccc atgctgttaa actataacca tgtaaagtag
     1081 ctaattcctt tgttccaaaa atatcactta gataagcagg taataaggaa aaacctgcac
     1141 cataacaagt cattaaaata gacatcgcta ttgcaaatac aatagcattg aatgacaaaa
     1201 ataaactaga agtcataata aagttcacaa taaataatat tataaaggtc aacgggcgtc
     1261 caatgtagtc agagagactt gcccataaca gacgtccaaa accgttaaat atccctagta
     1321 cccctactaa taaggctgca gattctgcgg aatagcctgc taaatcttgt gccattggtg
     1381 aagctgctga tattaaacct aagccacacg aaatattaat aaacaaggtc aaccaaatga
     1441 tgtaaaaggt ttttgatttt atagcgacgt ttgcttttaa tccagtgata atttgtgaat
     1501 tcatagcatt ctttttacca tcgtgagtca aaatagttat tttttcctga ggtggttgtt
     1561 taataaattg tgaggcaatc atcatgacaa aaaaatatac taatcccaaa atataaaacg
     1621 ttttacccac accaatccta atcagtaagg attgtgcaag cggacttgtt actaaagaag
     1681 caaagccaaa tcccataata gcgaatcctg ttgctagtcc cctcctatca ggaaaccatt
     1741 taataatagt cgatactgga gtaatataac ctgaaccaag tccgattcct cctaaaatac
     1801 cgtatgcaac atacagtaac cataactgct gagtttcaat ggctaagcct gttaacacat
     1861 tccctgctcc atataaaata gcagaaatca ttcccattat cctaggacca aaacgctcta
     1921 ctaagtgtcc cataaaagct gcagacattc ctagacaaaa aatagccaaa ctaaaagcga
     1981 atgaaactga tgaaatatcc caaccagtct ctgagataat tgggttacga aacacactcc
     2041 aagcataagt tgatcctagc attaaatgta aaacgactcc agaaaccgca actacataac
     2101 gatttaaatt tttcaaataa tcctcctaaa aacgaactta agttttattt ttaaaaatat
     2161 agttcgcaat aaacagatta tcaaaaaact gaatatatca ccagttttct gcttagcata
     2221 tctattttcc tcaataaaac tgaatattat tcgtgattaa aaaacaaaaa agttagggta
     2281 acttgttacc ctaacccctc tattatccta cttcaagaat atcaactatt ttagaaagat
     2341 tcattgagtt agaacctttt aataatattt gatctttctc cttaagagta tcttttactt
     2401 ttgcaagcaa ttgatcaaac tgatccactt cttgattctt tttaaagaaa tacactttac
     2461 caattgggaa catctggctt gctaattgag ctaacccctc aatatcctgg ccataacaaa
     2521 tcaaagtatc aatagagtca ggtctgatac tcataatcat ttgattatgt aaatccacag
     2581 attgttctcc cagttctttc atatcagcta aaagagcaat cttctttcca ccatcattat
     2641 tgggaatagc tgaaaaggtt tctagtatca atcgcatagc cgttgggtta gcattgtaaa
     2701 catctgataa aatatctgct ccattggctg atttcttcca ttcggtgcga ttacgagtta
     2761 gttgaagatt ttctagagca tccactatat cttcttctgc tactgctaat aattttccaa
     2821 cataggcagc gaccatcgca ttagttgcat tgtacttacc aggtactggt atacgtaatt
     2881 ggtgctccaa agcattagtt ttaaaagtta aggaatgttt ttcttctttg agttctgtca
     2941 cttgcaattc ttggtcatgg ccaaaacgaa tagtcatctg gtttgctgga agatatggat
     3001 caataattgg atctcccggt gcaattaata tgccatctga actcatacca tctgtgattt
     3061 gcattttccc ttctgcaatc ttttctcgac taccaaaaaa ttctaagtgt gcttcaccaa
     3121 tcaaagtaac aacagcaata cgtggttttg caatttcaga taacacatgg atatctccaa
     3181 gatgatcctg tcccatctcc aaaacaattt tttcagtatc ttctggcatg tgtagcacag
     3241 tatatggtaa gccaatctcg ttattgtagt ttccttgtgt tttataagtc ttatatgtgg
     3301 ttgataagat tgcagctatc atatctttag ttgttgtctt accatttgag ccagttactg
     3361 caataacatc aacgttcatc ttttcaatat aatactgtgc caagacttga aatgctttaa
     3421 gtgcatcaga cactaaaaga taaggatgac cctctatttc cttctctgaa atggttgcta
     3481 ttgctccatt atcaaaagcc atctctataa actcatgtcc atctcttgct ccttttaagg
     3541 gaagaaataa gtcaccttca ctgatattac gactatcaaa ttcaatattt cccaggggaa
     3601 cgtcttcaaa ctctgatact tgatttttag cgccaactac tttagcaact tcatgcaaac
     3661 taagcttcat atcatttcct tttctattat ttagcaacaa tgttgtagat agtatgctat
     3721 cccttataaa cgaatatact cctaaattat aacacacttt cataacatat catagatttg
     3781 aaaaagctca actaagaaag acttagttga acttttttaa attaaatgac tctcacgttt
     3841 ttcaaacatt tctttagcta acataactaa cttttcaatc aaatcactat aagttagccc
     3901 catattttcc caaagcagag gatacattga ccactgagta aaaccgggca ttgtattcag
     3961 ttcgtttaag aagatttgtc catctttcgt caaaaagaaa tcacagcgtg ataaaccaca
     4021 agccccgatt gctttaaaag ctttacttgc atattgacgc attgcttcca tagttgcttc
     4081 atcaacttta gctggaatat ccatagtaat tttattatca atatatttgg cgtcatagtc
     4141 atagaaatcg acgtctttaa caacttcgcc aggaaaagtt gtcttaacat cattattgcc
     4201 taaaatacct acttcaattt cacgagctgt cacgccttgt tcaatcaaaa tacggctatc
     4261 atacttgaga gctaagtcaa ttgcagagcg aagtgaggat tcatctgtcg cttttgaaat
     4321 acctactgat gaccccatat tagccggttt tacaaaaatt gggaaactta aagtttctaa
     4381 agagagttta atcgcatgtt ccaaatcatc accctcaaaa taagtttgat atgcaacctg
     4441 aggtacacct actgttgcaa ggacttgttt tgttgtaatt ttatccatag ccacgcttga
     4501 agatagaata ttagtcccaa cataaggcat ccttaaaact tctaaaaatc cttggataga
     4561 accatcttcc cccattggtc catgtaaaac ggggaaaaca attgcattat catcatagat
     4621 atcacttgga cgaaccattt tgtctaaatc aacagtttgg tttgtcatta acttttcatc
     4681 tgaagatggc atttcatcaa attcttgtgt tttaataaat tgacctactt gcgtgataaa
     4741 ataagtttta acaaaaaatt tatcataatt aatagcacgc atgacgcttt ctgcagacag
     4801 tacagatact tcacgttccg cagagcgacc accgtaaagc aagatcaatg tttcttttga
     4861 catatttctt ctcttttcta tctaatctaa gattgacctt ttaataatac catattttaa
     4921 cttaactttc aaaaatcaat ccacttaaag ttagtattgt aaaagaaaaa gttaggagat
     4981 tcccaacctt ttctttataa caattataac tctgtacgat tttcgattgc tcgaagcaag
     5041 gttacttcat ctgcatattc gatatctgaa ccaacagcta aacctctcgc taatcgtgtc
     5101 actttgatac cagctggttt taagactcgt gaaatataca ttgatgtagc ttcaccatca
     5161 gcagtcgcat tggtagcaac aataacttct gtcacctgac catccatcaa tcgagtaatt
     5221 aaacttttca aattaatgtc atcagggcta ataccattca taggtgaaat taagccatgt
     5281 aagacatgat acaaaccatt atactcttga attttttcca tagctgacac atctttacta
     5341 tcttcaacaa ccaagataac agactggtca cgcgtcttat ctgtacaaat taagcaagga
     5401 tcatcatcag tcaaattacc acaaacagaa caataggtta attcccgctt agcagctaat
     5461 aaattcttag caaattcatt aacatcttca tcactcatac caatggtgta aaaagctaaa
     5521 cgtgttgctg ttttagtacc tatacccggt aattttgaaa agctgtcaat caatttagca
     5581 attggtgttg ggtaaagcat tcaaatgtcc tctcttattg tcctgtgaac tgtgaaatat
     5641 attgatcaat catatctcgt gcaactaatt gatgggattt tgttgtatca gttgtaacat
     5701 gcggatacat taccgctact gctattttac gattagtatc gtaggcaata gcgtttaagt
     5761 tgtaggtaga aactgtttgt ccatttatat ttttagcaaa tgtttcagcg gtaccagttt
     5821 taccactaat ggttgtcaca ttccccctca ttgacgtacc agttgcatag ccacttccac
     5881 tattgacaac gttataaaaa ccttcttgaa taatggctaa ctcttgatct gagatagcta
     5941 tcttatttag tgttttagga gtgattgaac gaaccaattg agcgaactta ttagaatcat
     6001 tcccttcata gatatctgaa accacgtgag gtgctaaacg attcccatta ttcgcaatag
     6061 ttgagatata ctgaccaagt tgcattggtg tataggcatc atactgaccg aacgattcca
     6121 ttagagttgt tcctaagctg tattttccag gtacataacc ttctgattca ggtaaatcta
     6181 atcctgtaga aacccctaaa ccatattcgc cgtaagtttt ccttagttta gccattgctt
     6241 cttgatagcc tcgatcagtt aaagcatctc ctgtattata gtcttgtccc attaaacgaa
     6301 gtgctacttg aaccatataa gtattagatg aatactctag cgcttgtgcc gcagatattg
     6361 gagtcaaacc tcgtgtaaac catgaccgta tattagcaat ttcttggtca taaagcactt
     6421 catttcctct aagaacttta ttttcccatc ctgatgatag cgtcgcacct ttcacaacag
     6481 atcctggcgt gaaattctta gcaatagtgc ctaggctgtc atcaactaaa ttcccatgat
     6541 catttttcag cccagccatt gctaacactt taccagttga aggttctata gcaactgcat
     6601 acatcccttc ggaaaaacta gcgcgcccac tagataattc actgccatag tattgtttca
     6661 agatactttc tactttgttt tggtaattaa gatcaatcgt taatttaaga tttcttcctg
     6721 acttaccttc ttgcgtaata ttatctgaaa caactttacc ttttttatta acaactactt
     6781 tgcgaatttg tctgattcct tgtaagtcat cttcatattg tttttcaagg taagaagttc
     6841 ccactcggtc attcagtgaa tagccttttt tcaaatattt tttaacttct tctcttggta
     6901 aacctgcctt ttctgtagaa atagtaccta atatatccga taaagaagtt ccttttttac
     6961 gacgatgcca attacttgtt acagaaattc ctaacagttc cttcttatca gcatccagtt
     7021 tcttgatttg atcatcactt aattcacctg tacttaattt cactgaacca aacgttggtg
     7081 tcgcattcat ttggttaaat aaggcaacca cttttaactc atcttcagaa tagttaatag
     7141 cgctcactgg aactgcagca actgcatttg catatacggt tgattcggct agatggttac
     7201 caaatttatc gtaacgttta gaatctggta aactttcaac aacttttttg tagttggctt
     7261 tatctgctaa ataataatct ctctttgctc tatcgcttga agcaacttcg gttaaggtaa
     7321 tgtaatgtga taatttattc gctaactctt tgatagactg agatgaaata ttgtttcctc
     7381 tagaaaacgc cacagtagga cgttcatcat tttcaactaa gacaactccc ttagcatcaa
     7441 aaatctgacc tctcgctttt gattctttca ctgtataact cgtaagagct gtcagttttt
     7501 tcatgtaaaa tgattgttgc ccaatctgca tttgctctaa acgaagaatt agaacagtaa
     7561 ataataaaac aataataaaa aatagaagat ttaatcgtct aggtatgcta gcattttgct
     7621 tttttacagt gagacgatac ctttttttac gattcaacat taactccttg ctataaaaac
     7681 tctgactact attgtatcat aaaaatggtt ttttacagac actcaaaatg ttataaataa
     7741 taaaaaattc aagccaactt ggcctgaatt ttttatataa agattagatt atttacctaa
     7801 gtaatattct gaaacaaggt ttaatttttc atcaaattcg aaaacaagtg gtgggaagtt
     7861 aggaatttca acgtccatga tttcatcatc tgacaattgt ttgatatgtt ttacaagagc
     7921 acggattgag ttaccgtgtg caccaacaaa cacattttta ccatctttaa gagcaggagc
     7981 aattttatct tcccagaaag gaagagcacg ctctaaagta acttttaggt tttctgcatc
     8041 tggaataaca gaatcatcta gtgaagcata gcgacgatca gtatgtgctg aatgttcatc
     8101 atctttagcc atatctggag gcaatacatc atatgaacga cgccaaatat gaacttgctc
     8161 atcaccaaat tgttcagctg cttctgcttt attttttcct gtcaatccac cgtaatgacg
     8221 ttcgttcaag cgccatgatt tttcaactgg tacccaaagt tgatcagctg cttcaagggc
     8281 aaggttagtt gttttgatgg cacgtttaag aactgatgta aaagcaaggt cgaactcaat
     8341 acctgctgct tgaattaatt tcccagcatc aatagcttgt tgtgtacctt tttctgaaag
     8401 atctacgtca gcccatccag tgaaaaggtt agctttattc cactcagatt caccgtggcg
     8461 tgcgaatact aattttacca ttagaaaatt tctcctttaa ttttggctat accaatattt
     8521 actttaacta ttctacaaaa aatagccata aaaatctagt ctgattacta gttcaataat
     8581 caaaaatagc ttattattaa ctatctataa gaaaaggcat atcacctaga gtgctatgcc
     8641 tttagatatc atgtgattta tttaacaaaa tctaataagg caaggaagct ttctgcttca
     8701 agtgaagctc cacctacaag ggcaccgtca acgtctgggc aagccatata ttctgcaacg
     8761 ttttcaggtt taactgaacc accgtattga acgcgaactt tgtcagcaac tgcttgaccg
     8821 aagtctgctg caacaacgtc acgaactgct ttacacatgt tttgtgcgtc gtcttgagtt
     8881 gctgatttac cagtaccaat agcccagatt ggttcgtaag cgataacaag tgatgatact
     8941 tgctcttctg acaaacctgc aagagctgct gaaacttgag cacctacgaa ttcaaccgct
     9001 ttaccagctt cgtaagtttc aagagactca ccgcaacaaa tgattggagt caaaccatta
     9061 gcaaagatag cttttgcttt tttgttgata tcttggtcag tttcatggaa ataatcacga
     9121 cgttctgagt gaccgataac aacgtagtct gtacccattt cagccaatac tttagggctg
     9181 ttttcaccag tgaatgcacc tgaattctca aaatagctat tttgagcagc gattttcaat
     9241 tctgaacctt tagctgcttc aagaactgtt gataaagtaa gtgctggagc agcaatccct
     9301 gcttcaacaa gttctgatga tggtaattta gaagctactg cttcgatgaa tgcttttgct
     9361 tcttctggat ttttattcat tttccagtta ccagcgataa atggtttacg tgacattaca
     9421 catacctctt ctttttttat ttactaatct atcataccat aaattatagt taattaaaag
     9481 tttcacgagt ctagtaaaac gctttaatta tagatactaa aaaagagtcc ctgaggactc
     9541 ttcacaattt gagctgtctg actctaactg ccgtttggga acttaattaa gttaaattaa
     9601 gcttcgattt ctgaaacgat acctgaacca acagtacgtc caccttcacg gattgagaaa
     9661 gtagtacctt gttctacggc gattgggtga atcaattcaa cttcgatagt aacgttatca
     9721 ccaggcataa ccatttctgt tcctgctgga agttcgattg aacctgttac gtcagttgta
     9781 cggaagtaga attgtggacg gtagttgttg aagaatggag tatgacgtcc accttcttct
     9841 ttagaaagga tgtaaacttc acctttaaat ttagtgtgtg ggttgattga acctggttta
     9901 gcaagaactt gaccacgttc gatttcatca cgttgaacac cacgaagaag aacaccaacg
     9961 ttgtcccctg caagaccttc gtcaagttgt ttacggaaca tttcaacacc agtaacaact
    10021 gctttttgga tatcttcttt aataccaacg atttcaactt cgtcgttgac acgaacagta
    10081 ccacggtcga tacgtcctga agcaactgta ccacgtccag tgattgagaa tacatcttcg
    10141 actggaagaa gtaaaggttt gtcagtatca cgttctggtt ctggaatgta ctcatcaaca
    10201 gtgctcatca attccatgat gatgtcttcg tatttttcat cgccttcaag tgctttaaga
    10261 gctgaacctt ggataactgg aaggtcatca cctgggaagt cgtattctga aagaaggtca
    10321 cgaatttcca tttcaaccaa ttcaagcaat tcttcatcat caacaaggtc aactttgttc
    10381 atgaatacga taaggtgttt aacaccaact tgacgtgaaa gaaggatgtg ctcacgagtt
    10441 tgtggcattg gtccatcagt tgaagcaact acaaggatag ctccgtccat ttgagcagca
    10501 ccagtaatca tgtttttaac gtagtccgcg tgtcctggag cgtcgatgtg ggcatagtga
    10561 cgtttttcag tttcgtactc aacgtgtgca gtgttgattg tgattccgcg ttcgcgctct
    10621 tctggagcag catcgataga agcgtaatct tttggttggt taactgaagt aggcaagcga
    10681 cgtgccaata cagttgtgat agctgctgtt aaagtagttt taccgtggtc aacgtgtccg
    10741 attgtaccaa tgttaacgtg tggtttacta cgatcgtatt tttcttttgc cattttagga
    10801 aaagcctcca ataaaatata ttttatagat agacggtagg caatacagtc taactttact
    10861 tatctatttt aacaaaatcc tctaaaaaag caagtatttt atgctttttt gcttatttct
    10921 ttatttcctt aaaattcggg taagattgat tgaggcgttt gttatctctt atatgtattt
    10981 tttagtagtt
//
LOCUS       DATRME010000064        10904 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRME010000064 DATRME010000000
VERSION     DATRME010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            225..1442
                     /locus_tag="VBC23_001606"
     CDS             225..1442
                     /locus_tag="VBC23_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6843542.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            1426..2268
                     /locus_tag="VBC23_001607"
     CDS             1426..2268
                     /locus_tag="VBC23_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6843543.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            2345..3172
                     /locus_tag="VBC23_001608"
     CDS             2345..3172
                     /locus_tag="VBC23_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6843544.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(3212..3568)
                     /locus_tag="VBC23_001609"
     CDS             complement(3212..3568)
                     /locus_tag="VBC23_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6843545.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(3570..4046)
                     /locus_tag="VBC23_001610"
     CDS             complement(3570..4046)
                     /locus_tag="VBC23_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6843546.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(4101..5251)
                     /locus_tag="VBC23_001611"
                     /pseudo
     CDS             complement(4101..5251)
                     /locus_tag="VBC23_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(5324..5872)
                     /locus_tag="VBC23_001612"
     CDS             complement(5324..5872)
                     /locus_tag="VBC23_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6843547.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(5940..7031)
                     /gene="serC"
                     /locus_tag="VBC23_001613"
     CDS             complement(5940..7031)
                     /gene="serC"
                     /locus_tag="VBC23_001613"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO6843548.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            7164..7799
                     /locus_tag="VBC23_001614"
     CDS             7164..7799
                     /locus_tag="VBC23_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6843549.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGIKQAHGTRITQ"
     gene            complement(8069..8938)
                     /gene="rsmI"
                     /locus_tag="VBC23_001615"
     CDS             complement(8069..8938)
                     /gene="rsmI"
                     /locus_tag="VBC23_001615"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6843550.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
     gene            complement(8938..9264)
                     /gene="yabA"
                     /locus_tag="VBC23_001616"
     CDS             complement(8938..9264)
                     /gene="yabA"
                     /locus_tag="VBC23_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6843551.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(9294..10157)
                     /locus_tag="VBC23_001617"
     CDS             complement(9294..10157)
                     /locus_tag="VBC23_001617"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6843552.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(10177..10812)
                     /locus_tag="VBC23_001618"
     CDS             complement(10177..10812)
                     /locus_tag="VBC23_001618"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6843553.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
BASE COUNT         3425 a         2053 c         1707 g         3719 t
ORIGIN      
        1 acagttctaa ataaattatt aagcaatgat aaatgtagtt ttagtatatc atagcctcag
       61 cattgaagca atctcacttt atgaaaggat aaagaaaatc tcataagtca tttaccctta
      121 atttgaagtt taattagtta cttttaattt tactaccaaa aaacttgcac cattcttaac
      181 atttgttaca ataaatacaa cctattagga ggtcagtaca gattatgcac agcactattt
      241 ataaatgtca tcattgccgt tatctaattt ataaaagttt taaagatatc tttccttttg
      301 ctctcatttt ttcttggatt gaaatccttc aaaaactcgt tatatcagat gattcttttt
      361 tagctcaaac aacaaagtcc agtatttata aaaacgagtg gttatatacg gtttccaata
      421 ctctttatga tttatctcat ttactaatct actttatggc tagttatttt gtggctagtt
      481 tagtatggag aggcttatct aaccaaacta aagaaagttt ttctgcaagt ttacttggct
      541 ttgccactgt ttggttgctt ctaacttcag ttgttccagg tacagattat cactatccta
      601 gccaacccat ttggcttatg ctggctttga tttcgtatct ttttatcttt ttaactcgac
      661 tgcttcaaaa tcgacatcat attttccagt atagtatgtt agctatacta ctattaatct
      721 cttctatttt gtcacatatc ttatatagtt atcctaatat tgatttagaa taccttatac
      781 aaaatcactt ttctcaatgg ctgggtgatg ggccacatca attaattcct attatgcttt
      841 ggtctatctt agctattttt ctaaccttat caggtctttt tgtaccagat attttatttc
      901 gtccttactc ctatttttca gtagttagcg aaaatttaaa tgctgccttg agccaacata
      961 cagataaaat cccttatctg tatacctttt atactgttaa aaatagcttt gctatgtttg
     1021 gcggtattgg aatcctatta tccttatttt tagcagtttt atatgaaagt agaaaactac
     1081 aatcgaagaa ttactataaa ttgacattac tgaccttaac tcctcttatt tttgatcaaa
     1141 atttaccatt tttagtaggt ctacctgtca ttttacaacc aatattattc attccgatgg
     1201 tattaacaac tatctttgca gaggcttttg gagctcttat gctctaccta aaatttgtcg
     1261 accctgcagt ttatactgtc ccaagtggta cacctagtct actatttggc tttcttgctt
     1321 caaatgggga ttggcgttac cttccagtca ctgcaattat tctagttgtt ggcttcttta
     1381 tttatcgtcc gtttgtcaaa atcgctttcg ctaaggagga gcaatatgaa aaaattactt
     1441 agatggcttc ctcctgtact tttcattatt atccttatag gaatgactat cttaggtaag
     1501 tcctatatca ataaagtaac agctcacaaa ataaaactct ataactctcg aatgactcct
     1561 actattttaa ttccaggatc cagtgctact caagaacgat ttaacagcat gttagcacag
     1621 ctcaaccaaa tgggagaaaa acatagcgtt ttaaagttaa ctgtcaaaaa agacaatagc
     1681 attatctaca atggacaaat tagcggcaat gaccacaaac cctacattgt cattggattt
     1741 gaaaataatg aagatggtta tagtaacatc aaaaaacaaa caaaatggct acagattgct
     1801 atgaatgatc ttcagaagaa atataaattt aaacgtttta acgctatcgg tcattcaaat
     1861 ggtggcttat catggactat tttcctagaa gattattacg actctgatga atttgatatg
     1921 aaatcattgt taacaatggg aacacctttt aactttgaag aaagtaacac ctcaaatcat
     1981 actcaaatgc ttaaagattt aatcagtaat aaaggaaata ttccatcaag tctcatggta
     2041 tacaatttgg caggaactaa ttcatatgat ggtgataaaa ttgttccatt tgctagtgtg
     2101 gagactggta aatatatttt ccaagaaacc gctaaacact atacccaact aacagtaact
     2161 ggtaataatg ctacacattc tgacttgcct gataatcctg aagttatcca atatgtcgca
     2221 gaaaaaattc ttaaaaatga gaaaggtaaa ttaccaaaac ctcactaaag ctgtagaata
     2281 gcaacgcctc tggtattttg atataatata ttgcgaatta aactaaataa aaaaggatat
     2341 tattatgaaa ctcatttcat ggaatattga ttccctcaat gcggcattaa caagtgaatc
     2401 aacacgtgct ttaatgtcac gccaagttat tgatacttta gttgctgaag atgccgacat
     2461 cattgcgatt caagaaacta aactgtctgc caaaggacct actaaaaaac atttggaagt
     2521 acttgaaact tacttcccag aatatgattt ggtttggcga tcatcagtcg aacctgctcg
     2581 taagggctat gctggtacaa tgttcttata tcgtaaaggg ttaaatccta ttgttagctt
     2641 ccctgaaatc gatgctccta ctacaatgga taatgaaggt cgcattatta ctctcgaatt
     2701 ggaaaactgc tatatcacac aggtctatac tccaaatgca ggtgatggcc taaagcgctt
     2761 agctgaccgt caaatatggg atattaaata tgctgagtat ttagcaacat tggatagtca
     2821 aaaacctgtt ctagcaacag gtgactataa cgtagcccac aaggaaatag acctagctaa
     2881 tcctagcagt aatcgtcgtt ctgctggatt cacagctgaa gagcgccaag gctttacaaa
     2941 tctactagcc aaaggtttta cagatacttt tcgttacctt catggcgatg ttccaaatgt
     3001 ttatagctgg tgggcacaac gaagtcgtac atccaaaatc aacaatacag ggtggagaat
     3061 tgattattgg ctaacaagta accgtgtcgc agataaaatt accaagtcag aaatgattca
     3121 ttctggtgat cgtcaagatc atacaccaat tattttggag attgaactat aaaccaaaaa
     3181 aagatgattg agttataact caatcatctt tttatactaa gttgaggtct ttatacttag
     3241 tacggtagcc aatctgaaga attttattat ctttaactag caatggacgc ttaataagca
     3301 ttccatccga tgccaataag tccaaagctt ggtcaagagt taaatcatca aatttatctt
     3361 ttaagcctaa ttctctgtaa gaatttcctg aagtattgaa aaacttttta aggtcataag
     3421 gcgaattctc taacaattct tttaataacg aagctttggg aggattgctt tttatatcaa
     3481 tcgcttcaaa agtcaagccg agttcagtta actctttttt tgctgaacga caagtggtac
     3541 atttggggta ttcataaaac gtatacatat tatttttcct tttcgtattc taataaccaa
     3601 gattttcttt ctaagccacc agcataacca gttaattgac catatcttcc taaaacacgg
     3661 tgacaaggta taattagaga aataggattt ttacctattg ctcctccaac agcctgtgca
     3721 gattgacaat ttagctcttt cgctaattct ccataagttt tggtatctcc aaaagggatt
     3781 ttctgtaatt cctgccatac tctcgtctga aatgctgtcc ctctcaatga taatggaata
     3841 ctaggtaaat tttgctgtga aaaataagca tctaaccact tcttaccaag taaagtatag
     3901 gaatttgaca tattaacaat ttcctcttgg ttgactgcta gcatatcata tttttgccca
     3961 acaaaataaa gccctgataa acctagattg tctgctagaa ggcgaatttc tccaagaggg
     4021 gactgataga attcttgata taacatatga taagcataac ttaaaaagcc atgaaaagca
     4081 attcatgact ttttagttct ttattgttgt tttgcaatta aacgtacacg gacgatattc
     4141 tcatcacctt caaattcttc aattaaagta gaaatcttgt tattatccgt ttcatctaaa
     4201 tctattagtg tgtaggcgta gtcacccttt gaacggttaa taatgttatc aatatttata
     4261 cctaacactg atacagcagt tgaaatttta gccacaatgt taggaacatt cttgttaatt
     4321 aaagtaatac gaaatggtgc ggtttgaatt tgatgtacat tggggaaatt aactgaatta
     4381 gtgatttctc ctgtctccat aaaacaacga atggtttgac ttgccataat agcacaattt
     4441 agctctgcct catctgttga tccgcctaca tgaggaaaga ctgtaattcc tttttggttt
     4501 aataattctt tgtctccaaa atcagtaata tagcgcttga caacaccagt ttctatcgct
     4561 tcaaatagct cttggttatt gactaattct gcacgagcaa agttgataat cgtagttcct
     4621 tttttcatga ttgaaaaagc ttccgcatca aaagtatgct tagtttcatt tgttaaggga
     4681 acatgaattg tgatatagtc acaagtttca aaaatatcct taatctcttt aaccctttga
     4741 acatggcttg aaatattcca agctgtttca attgaaatat agggatcata accaagaact
     4801 gtcattccta agcgtctagc acattcgcaa ttctagctcc aatggcacca aggccgataa
     4861 ctcccaattt ttttcctgca atttcattac cagcaaaagc tttctttcct gcttcaattt
     4921 gtttgggaat atctgttcca gttagagtat taacccatcg gttagctcct aaataatcac
     4981 gagctgaaag taataaggca gcaatgaccg cttcttttac agcattagca tttgcacctg
     5041 gggtattaaa cacgactatt ccctgtgcac ttgcctcttc aataggaata ttatttgttc
     5101 ctgcacccgc cctagcaata gctttgaggt tacttgggaa atcctgatta tgcaaatttt
     5161 gactacgaat aatataggca tcaggatttt ctgacatatc cccatcaatc tggaaacgat
     5221 tccctaactc ttgtaaacca atttgattaa tattattaaa tgttttgaca ctaaggttat
     5281 ctctttagtc atcattgacc aaaataaccc ctttcttttt ttcttacatg ggatttcttt
     5341 catcaatctc acgatagatg ctcgtttctc ccttttcttt tatcttctga taagccaatc
     5401 gtaccccatc tagaggtact ttaccacaat attgatagcc taatttattt agaatatgct
     5461 gcatagtgac atttttttca tgagtatcgc aacgaaaatc aggtccttta tgaccttcaa
     5521 ttaacccttg aaggaatgtc tgtgctagtc ctctaccacg aaattgattt gagatcgcaa
     5581 tacgatgaaa tgtaagatag cgatggttat catggagcca ttttccctca taaatagcat
     5641 cataaacctc ttcatgacca tcaatgacag ccgcataagt cgctagcatc ccatcttcaa
     5701 taccaaccca agcataaccg ttcaagatat catcaataat atcgttacga ttagggtatc
     5761 catcttcttt ttgccattga tcacttcctg ttttagcaat ttcggcacgc gcttcctcaa
     5821 taagaagcat aatttggtca atttcatttg gaaaagctaa tctgatttgc attatattac
     5881 ccacttttat atattataga ctataataac ggtttaattt tctttcgtga taacatttac
     5941 tatgtatttt ctgcttcaaa tgttttcata aaatcaatta attcgatgac accttgttta
     6001 ggaaaggcat tataaaggct agctctcata ccacctaccg aacgatgtcc tttaatattt
     6061 ttaaatccac gttctgtcgc ttcctttaca aatttttcat ctaaatcttt attaatagtt
     6121 atgaaaggaa tgttacatag agaacgactc tttttatctc tgacaggatt actataaaat
     6181 tctgaggaat caatataatc atagagaagt cctgattttt cacggttagc tttttccata
     6241 gcatcaacac cacctagact tttcacccat tcaaagacta atttagcaat gtaaatactg
     6301 taagcaggcg gcgtattata aagagaacca gcatctgact gaaccttata atccaacatt
     6361 gatgataaag ttggttcctc attaatcata tcttcacgga taataactac cgttacacca
     6421 gcaggtccaa tatttttttg agctcctgca taaatcattg caaaatcttc tactttatat
     6481 tttacagcta atatattaga agacatatcc gcaataacag gcactccatt agttttagga
     6541 atatcataaa gcgatgttcc ttcaatggta ttatttgttg tcacatgaac ataggcagct
     6601 ttaggatcaa tctctttttc atcaaactct ggaatataat cataaactga ttcttctgat
     6661 gaagctaata gaattggttc aaaaggaatt gtttttgata gttttacagc ctctgtataa
     6721 gcttttttcc cccacgaacc agcaacatga tagtaagctt tacgccctct ggtaatattc
     6781 aagggaatca tactaaactg taaggatgca ccaccttgta aaaagattac tttataatta
     6841 tccggaattc ccatcaaatc tcttaagtaa cgctcagcac ctttgataat atcatcaaac
     6901 tctttggagc ggtgcgatac ctccaaaaca ctcatactag acccttgata attaaggagt
     6961 tcagattggg cttttacaag cactggtttt ggtaagactg caggacctgc agaaaaatta
     7021 taaattatca tataacacct ccatataatt gtgtaagatt atagcacagt attttgaata
     7081 ttgtaaagat ttttttgatt aattttgctt tttattcgtg atataatggt aaaggcgaac
     7141 aatcaagttt aaaggagtaa tttatgattt tgagggaatt ttgtgcagag aatttaacag
     7201 acttaaccag attagataaa gctattattt cacgtgtcga gctttgtgat aaccttgctg
     7261 tcggaggcac tactcctagc tacggtgtta tcaaagaagc taatcaatat ttacatgaaa
     7321 aaggtattag tgtcgctgtt atgattcgtc cccgtggagg aaattttgta tacaatgatt
     7381 tagaattacg tattatggaa gaggatatcc tacgtgctgt tgagttagaa tcagatgctc
     7441 ttgttttggg aatattaact tcaaataatc atattgatac tgaagccatt gaacaacttc
     7501 tacctgctac ccaaggtttg ccacttgttt ttcatatggc atttgatgtt atcccgaaat
     7561 ctgaccaaaa aaaatcaata gatcaattag ttgctttagg ctttacaagg attctcctac
     7621 atggttcatc taatggcgaa ccaattatag aaaatatcaa acatatcaaa gcattagttg
     7681 aatatgctaa taatcgtatt gaaattatgg ttggcggcgg tgtgactgct gaaaactatc
     7741 aatatatttg ccaagaaact gggattaaac aagcccatgg tactagaata actcaataaa
     7801 aaattcgaaa cagggctata agtttcttta gtatatttac tactaagttc tctcatgttt
     7861 tgtaaaacct gaaagaaccg gaataagagc ataccatctt ttataaccta ctctcagaaa
     7921 taaaatccaa tttgcaatgg tattcaaaag agctgaaatt aaaaatttta aggaaattgg
     7981 aagtatctta cttctttatt ccaatagcta aatcggagat tataaaaggt tgccaaattt
     8041 ttctttggca acctttttat attattactt ataaatcatg gaaactagca tagagttctt
     8101 gtctattgag attaaattct tttgctactt tttttatcgc ttgattagtt ttatcaccat
     8161 tagcgatata ttcttttact aatactagtg gatcttgttg gctactgtct ttcactcgct
     8221 cggtatctct cttaccatca acaataatta agcattcacc tttgagaggg accttttcaa
     8281 tatgctctaa aagttgacta atggttcctc tttgatactc ttcatagagt ttcgtcaatt
     8341 cgcgtactaa aacaacttgg cgatctccgt aaatctcttt catgtgtttt agcgtatcag
     8401 agactcgaaa cggtgactca taaaagattt gtgtttcagg gtaatcttgc tttgtttcaa
     8461 agaaagttat ttgttgacct ttcttacgtg gtaagaagcc ataaaaaata tgaggttgtg
     8521 gagctaaacc tgaagcgatg agagcagtaa taccagcgct agctcctggt atagatacaa
     8581 ctgggatatc cccttcaata gcagccttga caaggtcatg tcctgggtca gaaatagagg
     8641 gcattcctgc atcagatact tgggctaaag atttcccttc ttttaacaaa tcaattaacc
     8701 cagagatttt atcgtaagca ttgtgttcgt gaaaactaat ttgtttagta gtaatatcaa
     8761 agtgcttgag taaaagtccc gtatttcgtg tatcctctgc acaaataaaa tcaacttctc
     8821 ttaaaatcct aatggcacga aaagtcatat catctagatt accaattgga gttgggacta
     8881 gatagagtgt tccgtaatgt atatttgatt taaaactttt ttgaacttgc atttccatca
     8941 atctctatat aataattcaa tacaaaaagc acattcttcg tcattttcac gacgttgacc
     9001 atagaaagta ttacaaatgt gaaagccatc attgtaaatg ccttcgagta attctcttcc
     9061 ttgtttagaa tttttaactg cagtttctgt ttctgcttca ataacgctca gacgctcacg
     9121 aagtttacca ttttcaatgc gtagaacagt attctcctcc aataattttt ggatttgttt
     9181 tttcatagtc tcaatttcag acaacccaac taataaattt tgtgaaaaat catcaaaggc
     9241 atcaaacaag tcttttttat ccattcactc ttcctctctg cgcgtattat tttctaagaa
     9301 agcaccatat actctaaact attttgaaaa ctaacattgc tcttccacat ttgctgagct
     9361 ctataagtat tttctaaatc ataaagttgg ccttctttag ccagcattat tgtcagccat
     9421 tgaaaaacaa atgcctgatc atttttatct acaacttgcg atgtcaagcg agatacttct
     9481 aaataagcag tttgcctatc ttttgccaaa gtcgtcacaa actgttgact taaattcaag
     9541 taatctaata actttttgtt agtcaacaat atttctaaat gtttaggatc gtctgctact
     9601 tgagctaata agctagcttg tgattttagc aatcctgctt gttcagcttg atgaaccaac
     9661 atgtccaatt gttttggaaa acgaaaaatt tgcgttctac tcttaattgt tggcaaaaca
     9721 ttgttttcat cattagtcaa taagatgaca taagatgaac tttgaggttc ttcaataaat
     9781 ttcaaaagac tgttggctgc attgacatgc attttttcgc aatatttgat gataaagacc
     9841 tgtgatttcc cttcgaaacc tgatcttgaa aaatctttag tcagctcttt tatagtctct
     9901 atcttaataa gttgcccctg tggttctatg attttaacat ctgaaaattc cccattagcg
     9961 attaaacgac aggctctaca ctcttgacat ggtaaaccac tctggcgctt ttcacagaac
    10021 tgactttgtg ctaaataaag tgccatatct agacttgcaa aatttcccga aaaaagatag
    10081 gcatgactca tcctatctga ctgtaaaata gtattaaact tttccagcaa tttgggttgt
    10141 gttcgcttta aatccatgac ctttgtactt cctgattcat tggttgatac gatctaaaat
    10201 aatagagaat gtctcagcta ttacttcatc tagctgttgg gaggcatcaa tcgtcacaat
    10261 gcggtttggc tccgaatccg ccaattcaag gtaaccttga cggacgcgtt gatgcatatc
    10321 taattgctcg agatctagac gatttacctc cctttgaaca cttttttgaa tacgctctaa
    10381 acctacttca gaaggaacat caaaatataa agttaaatca ggtttatgac tatcagtcgc
    10441 atagtcatta agccatttta tatgtgactt atctaatcct cttcctgatc cctgataggc
    10501 aacagaacta tcaataaaac gatccattag gactattttc ccttcttcta gagctgggag
    10561 taccttttcc actagatgtt ggcgtcgagc agccatgtaa agcaatagct ctgttttttt
    10621 atccatttgc gtgtgtttaa catctaaaat aatatgacga atttcttctg aaatagttac
    10681 gccaccaggc tctctggtgg tgagaatatc ttgtgataat ttttctctga gcaagggtaa
    10741 aacagcttct aaaacggttg ttttacccgc accatctggg ccctcaaaag aaatcattag
    10801 tccttttttc atggtacatc ctttatttat aattactact attctatcaa aatttatcaa
    10861 aaaaagacac ccacaacttg attgtgcatg tctgatattt ttaa
//
LOCUS       DATRME010000065        10829 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRME010000065 DATRME010000000
VERSION     DATRME010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1968)
                     /gene="secA2"
                     /locus_tag="VBC23_001619"
     CDS             complement(<1..1968)
                     /gene="secA2"
                     /locus_tag="VBC23_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6843554.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDL"
     gene            complement(1965..2510)
                     /gene="asp3"
                     /locus_tag="VBC23_001620"
     CDS             complement(1965..2510)
                     /gene="asp3"
                     /locus_tag="VBC23_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6843555.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(2503..4092)
                     /gene="asp2"
                     /locus_tag="VBC23_001621"
     CDS             complement(2503..4092)
                     /gene="asp2"
                     /locus_tag="VBC23_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6843556.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWFTSQYKTILSDDFGRVFSD"
     gene            complement(4094..5671)
                     /gene="asp1"
                     /locus_tag="VBC23_001622"
     CDS             complement(4094..5671)
                     /gene="asp1"
                     /locus_tag="VBC23_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6843557.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNPSLELKIVSYKNDYDMTSLKEQVKDKILSSKNPDYFF
                     TSLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYTQIAA
                     LSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVKKMGD
                     YTSGKILQRWRKLLEKE"
     gene            complement(5681..6934)
                     /gene="secY2"
                     /locus_tag="VBC23_001623"
     CDS             complement(5681..6934)
                     /gene="secY2"
                     /locus_tag="VBC23_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6843558.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            complement(7044..10463)
                     /gene="srr2"
                     /locus_tag="VBC23_001624"
     CDS             complement(7044..10463)
                     /gene="srr2"
                     /locus_tag="VBC23_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO6843559.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYNYNGLYTRIYNYSKESTADLKNSTIKIYK
                     TTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWYDIYWGQFIASNETYVIVVETPFTN
                     AVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNAQERKILSELVSSSESVSSSESVSN
                     SESISTSESVSNSESISSSESVSSSESISTSESVSTSESISSSESVSSSESVSSSESI
                     SSSESVSNSESISSSESVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSE
                     SVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSESVSNSESISTSESVST
                     SESISSSESVSSSESISSSESVSNSESISNSESVSSSESVSNSESISSSESVSNSESI
                     STSESVSTSESISSSESVSNSESISSSESVSNSESISSSESVSNSESISSSESVSTSE
                     SISSSESVSSSESVSSSESISTSESVSNSESISSSKSVSNSESISSSESVSNSESISS
                     SESVSNSESISSSESVSSSESISSSESVSSSESVSTSESISSSESVSNSESISSSESV
                     STSESISSSESVSNSESISSSESVSSSESVSSSESVSSSESISTSESVSNSESISSSK
                     SVSNSESISSSESVSNSESISSSESVSMSTTESLSESEVSGDSEISSSTESSSQSESM
                     NHTEIKSDSESQHEVKHQVLPETGDNSASALGLLGAGLLLGATKSRKKKKD"
BASE COUNT         3514 a         2021 c         1603 g         3691 t
ORIGIN      
        1 taaatcctta tcattagcaa tatcgttaaa attatcctga ataattttta tcacaccatc
       61 aaccacaatg gaactatcag ctattaattc atttcgtaat tgataaatat tttcacgctg
      121 tatacgcaag ctctctccga acttaatagt ttgctcccta gaagatacac ctttatcttc
      181 acttctgagt tgagcatttt taactatttg ataaaaaaag cgtttctttc taggtaccat
      241 atagtaattt gaagtttctt tacgagaata tttttttaaa tactttatca cccattccgg
      301 agaatgagaa acaattaatt catcctctag cgacacataa aattggctaa tccctgggtc
      361 tccttgacgc cccgaacggc ccctaagctg ccagtccata cgtaaattag gcatcctttc
      421 cgttcctata actgcaagtc ctcctaattc tctaacccct tcacctagtt taatatctgt
      481 tccacgacca gcaagaactg ttgcacatgt tactgaacct ttttgtcccg cttcttttat
      541 aatttgtgcc tctttaacaa cattatcagc tgttaaggta ttatgggcaa tgccttctcg
      601 cagcaataac tttgaataaa tgttagcaat ttcaactgtt ccagaaatta acaatactgg
      661 ctgaccttta ctatgtcttt gtttaacatc atctatagta gcttgcaact tttctggtaa
      721 gctaacatat attttatcgg caagatctac tcgtttgatc ggaaaatttg ttggtataac
      781 aataacaggc aggtggtaag tttttataag ttcatcttca gaagattttc cagttcctgt
      841 catcccagac aaccttggga acatattaaa gagactttga tacgttattg aagcaattgc
      901 cctagattca tttgtcaaag caacatcttc ttttgcttca atagcttggt gaatccctga
      961 ttgaagtttt gtgccttcca taacacgacc tgttgcgtta tctaacaatt ttacttcatt
     1021 atttttaact acataatcat aatctctttt atacaaacaa tgagcacgca atgctaggtt
     1081 tatatgtcta tttaattccc aatactcatc attatataat tctgaaatat tgaagtactg
     1141 ttttgcgtaa cttacaccct tctcagtaag atagacaatt ttatccttag ctttatggat
     1201 atattcatca ttttccttaa gagtgagtac aaaattatta gtgataccat agagattaga
     1261 ttgtacacga ggatctcccg ctataattaa gggtgtttga gcaatatcta acaaaacaga
     1321 gtctgcttca tcaacgataa caaaatgaaa aggtcttaga aattgttcta gtttattact
     1381 agccagattg tgaacgagat agtcgaaacc taaggcacct gcagtagtat aagtaatatc
     1441 agattcatat acttctctct taatagtcgc atctgcttct tcttttttat caaaaacatt
     1501 aataccaact gtcataccta gaaattgata tacttttccc atctctaatg catctcgttt
     1561 tgccaaataa tcattggtcg tcactaagat tgctcccttg ctagttaaag cattcagata
     1621 caagggcata gttgcagtta atgtttttcc ttctcccgtt ttcatttcag ctatcgttcc
     1681 ttgatgaaga gcaatagcac ccataacctg agcatcatat ggaaacaaac ctaaaattct
     1741 tttattagcc tctcttatca cagcaaaagc atcaacaaga atatcatcta gactttctcc
     1801 acttgaaagt ctatttttta atattaaagt ttgatggctt aactccttat ctgataattg
     1861 agcgtagtac acttttttat tatttatctc gtttacaatt ttttgaattc tttgcaatct
     1921 caatttattt agaatagaga ttttgggttt aacaacaact tttttcattt tttatcctta
     1981 gaaaatgcta tcaacgttct aataagttca agctctgatg gtaagctgct ttcaacataa
     2041 ctgtttagtt caataccatc tttttcttct tgagtctcaa ttacttcaaa taattctagg
     2101 taacaaaatt ctacacttat acaacccgca ccaataagag aaatatcata aaaatatgtg
     2161 tcatcaggaa ctataaaatc tgaggagtga tctcgaataa tagaaaaacc tattttttct
     2221 ttttgacgat tataaaaatt tatctgaata atcacgcatc cctcaggcac agtagttaat
     2281 tttgatacca actcataggt atgattaggt tttagtaagg taagctctgg caacgttcta
     2341 ttagcttgaa aattcgtttt agaaagccat tttttgattg tttttcctgt agagaatctt
     2401 agattctcaa aaaaaacacg attatgctcc aaatacttta tagtcgaacc ataaagataa
     2461 acattgctac tatcagtcca aagcaccttc tgtattcttt ttttaatcac taaacactct
     2521 cccaaaatca tcactgagaa ttgttttata ctgagaagta aaccaattat ttattgattg
     2581 actattatca ttatgtcgcc cagcatatcc acttgcatac aacagggtgt tagttttaga
     2641 aaaataatca atcagccttg gagttgcatt tctatcataa tcatcatgtt tcatataaga
     2701 tatagcaaac tgcgtttttt caaaattaga acgcttaaac ttattccaaa atttttgatt
     2761 aaattgatta atagcttcat cgttttgaga accaataata ttttggagaa tatccatact
     2821 cgtttcaaaa tcatctggtc ttcttaattt taaatttact accatatcgc ctaaatttgt
     2881 aaagggtttc cccacaacta tagcatgggg ggaaaaagta gaaccataat aaagggcacc
     2941 aaaacttccc atagataaac ctgataaaat aagttgacaa tgatcaaatt gtaggtaatc
     3001 taaatatttt tgtaaaacat tttctaattc actttcgtat tctgaagtac ctaggtagaa
     3061 gcttccacct tcaatccttg gatcaccaat taagagaaac ggagctccca aacgtttcat
     3121 catgtaaaaa ccctcaaaac cttcggctcc tcgaaaacct gaaaaataaa cattcaatgg
     3181 aggtttcata tctccagggt taaaatagta gataaactct tgccttttgg aatcactgta
     3241 cctttctcca ccgattaaaa attgacccaa acctttacgc gaatatcgcc aatgtagtgc
     3301 accaaatctc accactccgt cacctctagc aaagatagaa attgatgaaa aaccaactga
     3361 ttcatcatat tctaaaatat atggatcagc caaatcattt tctgaaaggt attttgtatt
     3421 taatatcaaa tcaagtgatc ctttttggta ttgaatgact tctatcataa tactattatc
     3481 accttgcaat ttttgatatt cttgccatat ttctatcgct ttatcagtag aggataaatt
     3541 atagcgaaat gagaatagag gttgaaaatc ttctccaaaa ttccctgaaa aacaaataga
     3601 gctattgcct tcatatatta tctctccctt aaaatcagga tttagatcta tgtcagaaac
     3661 cttcaatttt gcaccatatt gttccgaaaa aagagtacgg cttaggaaaa taattttttc
     3721 tttccttgat ccattaaatg gtagtggtct caaaacctta cgtttaaata taccgttttc
     3781 tgagcaaaaa tcgagttgaa aatcctcgtc gtaaaagagt gtatgcgctt ctaagcaatc
     3841 tgaaagtagg ttcaattgca attcgctaac ctcactagtt aaaatgaccg cattaaacca
     3901 taatttaact tcattagttg aagttttacc aatatattca acattatttt tttctaactc
     3961 tttacttttt aatttctcta agaaaatttg aatagcattt ccattacagt ataaccattc
     4021 aatattgtct ggagttatta aatcagcatc ccatggttcc tcaccaatct gcaaaatttt
     4081 taatttttcc atattattct ttttctaata attttcgcca gcgctgtaat atcttaccgc
     4141 ttgtataatc acccattttt tttacgctgt aaactaacga tttattccaa ttcattagtc
     4201 catcaaaata atagtcaatt gcattattga gttctgtaga gtcactaata atccagccat
     4261 tcttctggtg ttctacataa ttagtgtgaa ctttattgat ttgaggaatt ccagaactca
     4321 gagcagcaat ttgagtatat aggtctggat catctcttaa atcaataact aacctagcag
     4381 tatctagagc atctataatt tgattttcat ttgtatatat atcaaaacta atgccacgaa
     4441 ttttagtttt ttgttcttcc ttaaaatgat tctcatcttc caaagaaaga gaggtaaaaa
     4501 aataatctgg atttttagaa gacaatattt tgtccttaac ttgttctttt agagaagtca
     4561 tatcataatc atttttatag ctgactattt tcaactctaa tgacggatta acttccattt
     4621 ttgctaataa ttggtctaaa acttcactta aataagtttg agaaagatca tctacaagta
     4681 aatatgcaat aagctcttca atattttggc tctgtccgag tctcaaacga gtatcaaaag
     4741 ggggaatacg gctaatgtga ttctcaacat agtttgacga ataatttttc tcttgtaatg
     4801 aagtaatcaa taaccgttct attttttcgc tttcagcaat aaacaattca ccttcatcaa
     4861 gaagttcatt aaagtcgagt cgattatcaa atgaatagcg ttcaccaaaa aatgagaaaa
     4921 ttttcttttg atctggaaat gattttctga ctaaattgtt atgaatatcg tttactgcta
     4981 ctataatata atcatctgga gtcacttcta gtttttttaa aaccaatatt ctctctcgaa
     5041 taagagtatc ccaatcatca tactcagtaa aattaaacac cttatcagac attggattaa
     5101 ctactattct cggaaatttt gaagcatact ctcgaatttg ccaaacacca ttttgattta
     5161 aataatcttg ataaattaat ctattttgtt tgaaatattt gatactggat acaaaaccac
     5221 gatcgtcaaa tatcataatc ttatcaagca caccatctat taaaagttca atcagtagta
     5281 aattcccatt ttccgcaaat tggatacgtg caataatctc gccaccatgt ttagctaata
     5341 ctaaaaaagg tgtataaaca aaataagtcc ctttaggcca attcagttct ttaaaattaa
     5401 ttaacttaat ctctttttgt tgaatatttt gaatatcgtc aaaaaaagac caataagaga
     5461 cagccaacat actctcttca tgaagcatgt atctcaactg aggattataa ctaaatagaa
     5521 gtaaacgcac ttcctcatct gcattctgaa acattctaac ctgactaaca gtatcatcaa
     5581 aagtcatgtt ctccaataca cgataccaga taggagcttc attatgccac ttttgatcag
     5641 aattgtacca cgaaggaata aaataaaaca tgattgctcc ttaaataata tttcggtgac
     5701 tgccccacaa cttcaaggta ttaacttgct caataatact gagcataaaa gttgaaataa
     5761 tataaatatt aatgacgatc attgcaatac taacttggtt tgtcttcccc accacagcca
     5821 acattgggag acctcccata ataaccgtaa aaattgctcc aactactgcc atgatactaa
     5881 ttttttcttg taaaaaacgt tgagtagcag ctcctggagt tacattttga aaatattccc
     5941 cccgttgacg catatttttt gcaatattat acgggtcgta attaaaatat gcaaatccaa
     6001 tgctcaaaat aaataataat ataacaaaaa gtccaatacc taaaggctta ttaattgcaa
     6061 tcattgacga gatctgaatc aataatcgct cgtttggaaa agcaagtaac aatgcttgaa
     6121 ttataaacgt tggaagcatc attaatgtca tcccatacat gaaagggtag gaccctgctg
     6181 gatttatgcg aataggaata taggtatctt gggcaaattt actaacgata tcaatacgcc
     6241 taataggtat acgatattct gccctataaa ccaccgttga gattaataat aaaacactaa
     6301 gtattatact tatagctata atagttccca gcagatccat aatactaaaa tgtgactgtt
     6361 caaaataagt tattattcca gtagtcgttg cttggagcat gctcactaat atgataacaa
     6421 tacttcctcc tagcccttta gaagcattga gattagcaag ccacattaac acaaatgagc
     6481 cacttattaa caagaccatt gtcaatgctt taaaaacaat atcactataa gaacctaata
     6541 aagagctttt tataaattga tttcctgaaa caccatatgc ttgtataact gagatagtaa
     6601 gcattaaagt atattgagct acttctgctt ttcgcttcgt tgcatttttt gcccacgaaa
     6661 ataaggaaac gaatctccaa ataatcatac ttgacatcca tggactcaac cccagtgtga
     6721 aaacatttaa tttattaaaa ctcccaccac ttactgctga aaagttattg agtaaatgtt
     6781 gatctactgc tagttttatc tcttgatgtg ataaaggaac agtggataat aaaacacgtt
     6841 gtcctaacat atatacacca acaattaaaa tagtccataa aatacttctt aatagcgttg
     6901 actttctata gctataatat aattttccaa tcattttttc ccttttcaaa tcaatttatc
     6961 acttaagaca gtataactac ttactatctt aagtgataaa tcgaaagtac tttcgattta
     7021 tcatctaaat atcactttga aaattaatct tttttcttct tgcgagattt agtagctcct
     7081 aacaacaatc ctgcacctaa cagacctaat gctgaagccg agttatcacc tgtttctggt
     7141 aatacttgat gcttaacttc gtgttgagat tcggaatcgg atttaatttc agtatgattc
     7201 atcgattcag attgacttga tgattctgta cttgagctaa tttcagaatc ccctgatact
     7261 tctgattcac ttagagactc tgtagtactc attgatactg actctgaact tgaaatagac
     7321 tctgaatttg ataccgattc tgaacttgaa atagattcag aattagatac cgatttggaa
     7381 cttgaaatag actcggagtt agataccgat tctgaagttg aaatagattc cgagcttgat
     7441 actgactctg aacttgatac tgactctgaa cttgatactg actcggaact tgaaatagac
     7501 tctgaatttg ataccgattc tgaacttgaa atagactctg aagttgatac cgattcggaa
     7561 cttgaaatag actcggagtt agataccgat tcggaacttg aaatagactc tgaagttgat
     7621 actgactcgg agctagatac cgattcggaa cttgaaatag attctgagct tgatactgac
     7681 tcggaacttg aaatagactc ggagtttgat accgattcgg aacttgaaat agactctgaa
     7741 tttgataccg attcggaact tgaaatagat tcagaattag ataccgattt ggaacttgaa
     7801 atagactcgg agttagatac cgattctgaa gttgaaatag attccgagct tgatactgac
     7861 tctgaacttg atactgactc tgaacttgaa atagactctg aagttgatac cgattcggaa
     7921 cttgaaatag actctgaatt tgataccgat tctgaacttg aaatagactc ggagttagat
     7981 accgattcgg aacttgaaat agactctgaa tttgataccg attcagaact tgaaatagat
     8041 tccgaggtag atactgactc ggaagttgaa atggattcgg agttagatac cgattcggaa
     8101 cttgaaatag actctgaatt tgatactgac tcggagctag ataccgactc ggagtttgaa
     8161 atagactcgg agtttgatac cgattcggaa cttgaaatag attctgagct tgataccgat
     8221 tcagaacttg aaatagattc cgaggtagat actgactcgg aagttgaaat ggattcggag
     8281 ttagataccg attcggaact tgaaatagac tctgaagttg atactgactc ggagcttgaa
     8341 atagactctg aattcgaaat agattctgag cttgataccg attcagaact tgaaatggat
     8401 tcggagttag ataccgattc ggagcttgaa atagactctg aagttgatac tgactcggag
     8461 cttgaaatag actctgaatt cgaaatagat tctgagcttg ataccgattc agaacttgaa
     8521 atggattcgg agttagatac cgattcggag cttgaaatag actcggagtt agataccgat
     8581 tcggaacttg aaatagattc tgagcttgat accgattctg aacttgatac tgactcggag
     8641 cttgaaatag actctgaagt tgataccgat tcagaagttg aaatagattc tgagcttgat
     8701 accgattcgg aacttgaaat agactcggag ttagataccg attcagaagt tgaaatagat
     8761 tcggaattag ataccgattc tgaacttgat actgattctg agctacttac taattcggat
     8821 aaaatttttc tttcttgagc atttacatct gaatatcccg attcagatga gtaagtatga
     8881 ttatgttcta caccattgtt ctcattataa tctgataaag tagtattcaa agttactgca
     8941 ttagtaaatg gtgtttctac aacaattaca tacgtttcat ttgatgcaat aaactgcccc
     9001 caataaatat cataccaccc tttttctggg taactatttg caaacttact agttacatcc
     9061 tccatgcttg aataatcctg taccatactc tctacaatat tatccgaggt ggttttatag
     9121 atttttatcg ttgaattctt taaatctgct gtactctctt tgctataatt atatatccta
     9181 gtatataacc cgttgtaatt atagagtgat tttccatcta cattaatttg acttatataa
     9241 gtcatctttt ttcccacatc atcaatgttg gttattcgag aatctacact tactccccct
     9301 gcaaaatcaa ttcttaccgg tggatcatat ttcactgttt ctttaattgt agcctcttga
     9361 tcctttattt ttaccgaaaa aagaacatct tcttttgtgt tttgaactgt cgtatcatct
     9421 ggccataatg gaatatctaa tattccatta atattatttt ttgaagccgc aaaatctgta
     9481 aaactataga ctatagattt tttttgagaa tcataaattc cagtcgcata gacctcaccc
     9541 gtaggtgatt ttataggttc tacaatagaa tagttagaag ctccatttgt atcgatatta
     9601 tctgataact taatttcaaa agtatcacct ctattcacag aatcaatagt gaattttaaa
     9661 gacatgtgca tactttctga cttatccata tttataacac ctgcttttgc ttgcactcct
     9721 ccagagagct ttatatctaa attttgtaac ttatctgtta catcttgtcc ttgaacagtt
     9781 gttacatctc cttcaccaga gattaatgct gctggtaaag tttccatatc tgaagcacta
     9841 gatacaactc tcatcgctct cggttgaact ctagcggtcg ttgccgcttc tgatgattga
     9901 actgattctg atgctgagac actctcacta ctagatagag ataaagaact atattctgat
     9961 aacgagctag actcagataa agaagcagag gtagaatcca ttagtgattc actggcagat
    10021 aatgaggcgc tggtagattg caaggtacta gttgacattg attgtgattc acttgttgaa
    10081 tgtgatgaag aatctgagtt tacagttttt acttctggcg caaccactac agtttcagca
    10141 gtagctaatg ttttttcatt tcctgtaagt tctgttgcag taggtgtttc ttcagctgag
    10201 acagaagttg tgtctgtcgt ccctccaact acagctccaa gagctgcgac tgctgttagg
    10261 atgctagcag acgttttagt tctgcttaaa tcttcactag tagtttgaac cttagagctt
    10321 ttctctttac cgctaaacct cgaaaggata acatagccaa tttgtgtcat aacacttttg
    10381 acccagtgct tacctgattt cctcatttta actcgaactt tgcgtgattt ttcaccaaag
    10441 tttccaaact gcttttttaa catttttcct ccatatttaa aaaataatgc tatataaaaa
    10501 acttttctaa ttctaagaat tctcaaatat agctataaca aaatactact ttgttatttc
    10561 aagttctaaa atattgcttg aaacatcatc aataaatagc atatttgcct ataaagaccc
    10621 ctcctatctt aaaaagctac aataaacaat tcttcctctc ttctgaaaat taaatcaact
    10681 attgcttctt tctaatctaa ataaggtgtt tttaaaaaca cttttcgtat tataacatac
    10741 tttaatcaaa ttggatataa aatttcagtt tttttaacac ttatttttta ctactattat
    10801 tcaaagtatt tcatagtgtt cttcttcgc
//
LOCUS       DATRME010000066        10683 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRME010000066 DATRME010000000
VERSION     DATRME010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            260..1429
                     /locus_tag="VBC23_001625"
     CDS             260..1429
                     /locus_tag="VBC23_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6843560.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1426..1572
                     /gene="rpmG"
                     /locus_tag="VBC23_001626"
     CDS             1426..1572
                     /gene="rpmG"
                     /locus_tag="VBC23_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6843561.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1619..1855
                     /gene="secG"
                     /locus_tag="VBC23_001627"
     CDS             1619..1855
                     /gene="secG"
                     /locus_tag="VBC23_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6843562.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            1968..4373
                     /gene="rnr"
                     /locus_tag="VBC23_001628"
     CDS             1968..4373
                     /gene="rnr"
                     /locus_tag="VBC23_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6843563.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4376..4843
                     /gene="smpB"
                     /locus_tag="VBC23_001629"
     CDS             4376..4843
                     /gene="smpB"
                     /locus_tag="VBC23_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6843564.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4927..6315)
                     /locus_tag="VBC23_001630"
     CDS             complement(4927..6315)
                     /locus_tag="VBC23_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6843565.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6444..7370)
                     /locus_tag="VBC23_001631"
     CDS             complement(6444..7370)
                     /locus_tag="VBC23_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6843566.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7499..7891)
                     /gene="gloA"
                     /locus_tag="VBC23_001632"
     CDS             complement(7499..7891)
                     /gene="gloA"
                     /locus_tag="VBC23_001632"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6843567.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(7979..8653)
                     /locus_tag="VBC23_001633"
     CDS             complement(7979..8653)
                     /locus_tag="VBC23_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6843568.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8920..9762
                     /locus_tag="VBC23_001634"
     CDS             8920..9762
                     /locus_tag="VBC23_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6843569.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            9953..10675
                     /locus_tag="VBC23_001635"
     CDS             9953..10675
                     /locus_tag="VBC23_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6843570.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT         3649 a         1700 c         2070 g         3264 t
ORIGIN      
        1 tttaattatt ggctatttag cagttgtagt atttgctcca aaaactttcc gtttaaaaag
       61 ttaattttaa ttgtggtata ataacagagg catgtgataa cataggttta gttacataaa
      121 ggaaattata atggaaataa catctcaaat agatgttaaa tctcaataaa actgctaaga
      181 tagttagtgg tagaaaagtt tagaaagaag atgatgtttt atctcagttg aaacggttaa
      241 ctggaagcaa aatttaaaag tggcttggct cggtaatttt tttactgggg caagcttttc
      301 acttgttatg ccttttatgg tattatatgt agaacaatta ggagcaccta gcaacaaagt
      361 agagtggtat gcaggcctat cagtatcctt atcagccttg tcttcagctt tagttgcccc
      421 attatggggg cgcttagctg ataaatatgg tagaaaaccg atgatggtaa gagcagggtt
      481 gatgatgact tttactatgg gaggccttgc tttcattcat agtgttacag gacttctgat
      541 tttacgtata ttaaacggca tttttgcagg ctatgtacct aactctacag ctttgatagc
      601 aagtcaagct cctcaagagg aatcaggata tgccttagga accttagcga caggagtgac
      661 tggaggtatg ttaattggtc cacttttagg gggactttta gcagagtggt ttggtattag
      721 ggaagttttt ttattagtgg gaaccatatt gttaatatct actttaatga ccatttttat
      781 ggtaaaggaa gatttcaaac ccatttctaa cgaagaaacc atgccaacta cagaagtgtt
      841 caaaagcgtt aaaagcttac aaattttaat agggctgttt gtaacaagca tgattattca
      901 aatttctgca caatcaattg cacctatttt aacactttac attcgtcatt taggacagac
      961 tgaaaacttg atgtttgttt ccggattaat tgtttctggt atgggattct ctagtattct
     1021 gagtagtcca aagctaggaa gaatcgggga tcgcataggc aatcatcgtt tgctattatt
     1081 agctctactg tatagtttcc tcatgtatgt cttatgttca ttggcacaga cttcattaca
     1141 gctaggggtt attagattcc tgtatggttt tggaacaggt gccttgatgc cgagtattaa
     1201 ttcaatcttg actaaaattg ctccaagaca aggtttatca aggattttca gttataacca
     1261 aatgtttagc aatttaggac aagtattagg tccttttgtc ggttcagcag tttctattca
     1321 tttaggattc cgatgggttt tctttgtgac tagcttcatc gttttagcta attttgtttg
     1381 gtgttttatt aattttagaa aatatattag agttaaggaa attgtgtgag agttaaaatt
     1441 aatttaaagt gtagctcttg tggtagtaag aattatttaa ccagtaagaa taaaagtaat
     1501 catcccgata aaatcgaggt tccaaagtat tgcccaaagg agagaaaagt aacactacat
     1561 attgaatctt aataagtttt atgttagaat gtactttgac catagtggag gaattaacat
     1621 gtataaccta ttactcacca ttctacttgt tttatcagta ttattgatta tctcaatttt
     1681 catgcaacca caaaagaatc caagtagtaa tgtttttgat agtagcggtt ctgaagccct
     1741 ttttgaacgt agtaaagctc gaggatttga agcttttatg caacgtttta caggtgtttt
     1801 agtctttttc tggctactaa tcggcttagt attatctatt ttatcaagtc attaatgata
     1861 gctaaataca gataagtgag gatgggggat agcccaacct cactttttgt tagtagaatg
     1921 gttgtagagt aataataaag tgactaaagt tactagaaaa ggaaataatg aaagaaaaaa
     1981 ttataaaata tttggaagaa cacggtaaaa ccagcgttaa tgatttagct gctgccctag
     2041 atatggcagg cgcaaaagca tttccaaaat taatcaaaac aatctcaaat ttggagagcc
     2101 acaggcaatt aagatttgac gataatggaa gtctttctct tcaaaagaaa gaagctaaga
     2161 aaaaggaaat cacagtaagg ggtcttttta gagcaaataa agcaggattt ggttttctta
     2221 gtattgacca agacgaagat gatatgttta tcggaaaaaa cgatattgct tatgctattg
     2281 atggtgacac ggtagaagct gttgtaaaga aaccagcgga ccgtcttaac gggactgcag
     2341 cagaagcgcg tgttgttaat attgttgaac gtagtttaaa gacgcttgtt ggtaagtttg
     2401 tcttagacga tgagcgccct aaatacgcag gatatatcaa atcgaaaaat caaaaaatca
     2461 atcaaaagat ttatatacgc aaagaaccgg tcgttttaga cggaacagaa attatcaaag
     2521 tagatattga taaatatccg acacgaggac atgattactt cgtagcaagt gttcgtgata
     2581 tcgttggaca tcaaggagat gttggtattg atgttttaga agtcttggaa agtatggata
     2641 tcgtctcaga atttcctgag gacgttattg cagaagccaa tgctatccca gatgctccaa
     2701 cggagaaaga tttaattggg cgtgttgatt tacgtcaaga ggtcactttc actattgatg
     2761 gagcggacgc caaagattta gacgatgctg tgcatattaa attactggat aatggtcatt
     2821 ttgaacttgg agttcatatc gcagatgtat catactacgt tactgaaggc tctgccttaa
     2881 atcgtgaagc acttagtcgt ggtacgtccg tatatgtcac agatcgcgtt gtaccaatgt
     2941 taccagagcg tctatctaac ggtatatgtt cattaaatcc aaatcttgat cgtttaacgc
     3001 agtcttgtat tatggaaatt gaccaaaatg ggagagttgt taatcatcaa attacacagt
     3061 cagtgattaa taccacttat cgtatgactt atatcgctgt aaatgatatt attgctggcg
     3121 atgaggaaat ctgctcagaa tatgagtcta ttgtgtcctc agtacagcat atggtcactt
     3181 tacaccatac tttggaagct atgcgtacgc gtcgtggagc gctgaatttt gacacatctg
     3241 aagcaaaaat tatggttaat gataagggaa tgccagttga tattgttatt cgcaacagag
     3301 gcattgctga acgcatgatt gagtcattta tgttggcggc aaatgaaacg gttgctgaac
     3361 attatgctcg tctaaaactg ccatttattt atcgtataca cgaagagcct aaagctgaaa
     3421 aattacaaaa attcatcgat tatgcttctg ttttcggtgt tcagattcaa ggaacagcaa
     3481 ctaaaatcac ccaatctgct ttacaggact ttatgaaaaa agtacaaggg cagccaggaa
     3541 gtgaagtgtt atctatgatg ttattgcgct cgatgcaaca agctagatat tctgaacaca
     3601 accatggaca ttatggactt gctgcggaat attacactca ctttactagc cccattcgac
     3661 gttatccaga cttgttagtt catcgtatga ttcgtgatta tgatgataaa gcgatggata
     3721 aagctgatca ttttgctaac cttatcccag aaattgcaac tcaaacgtct agtcttgaga
     3781 gacgtgccat tgatgctgaa cgtattgtag aggcaatgaa gaaagccgaa tatatggaag
     3841 aatatgttgg tgaggaattt gaaggtgtgg ttgcaagcgt tgttaaattt ggtatgtttg
     3901 tagaattacc aaatactatt gaaggattga ttcacgtaac gaccttgcct gaatattatc
     3961 actttaatga acgtacgctg acgcttcaag gtgagaaatc tgggaaagtt ttccgtgttg
     4021 gtcaacaaat taaggttaag ttgattcgtt ctgataagga aactggtgat atcgattttg
     4081 attatttacc atcagatttt gatattgttg aaaaggtaag taaaagctcg cgtgagggtc
     4141 gtcctaatag aagttctaaa cgagaacatc agcatagaat ttctgatcgt gataataaga
     4201 ataaaaatac atctaagaaa aaagcatctc gaaaacccaa aaggaactcg gatagcaaat
     4261 cacatcatca taaagacgat agaacaacag gttcaaccaa gaaaaaaacg aaaaaacctt
     4321 tctataaagg cgttgctaag aaaggtcaga aacggaaatc taagaaaggt taagtatggt
     4381 taaaggtcaa ggaaacgttg tagcacaaaa taaaaaagcg catcacgact atactatcgt
     4441 tgaaacaata gaagcaggaa ttgttttgac aggaacagaa atcaaaagtg tccgcgcagc
     4501 gcgtattaca ttgaaagatg gatatgcaca aataaaaaat ggtgaagcgt ggttgattaa
     4561 tgttcatatt acaccatatg atcaaggtaa catatggaat caagatcctg atcgtacacg
     4621 aaagttatta ctaaaaaaac gtgagattga aaaaatatca aatgaactga aagggactgg
     4681 tatgactctg gtacctctaa aagtttactt aaaagatggt tttgctaaag ttctattggg
     4741 tttggcaaaa ggaaaacatg attatgataa gcgagagtct atcaaacgtc gtgagcaaaa
     4801 ccgtgatatt gcccgtcagt taaaaaacta taattcacga tgattatcaa aaggttaggg
     4861 aattgcctaa ccttttttgg acgaaaaaag tgaagttgat tgaccaactt cactaataat
     4921 atagacttaa tgtgatttat gtttgaagtg actatataaa ccaaatccaa taatccagat
     4981 ggttgctcca atagccccga tgtatgttga atcttgcaag aagagagaaa cgaatacaaa
     5041 gaggaagaag agaagtgtga ttggtgaaag gattttgtag gcaggcattt tataaccatc
     5101 ttccataaaa tcaggtgatt tacgatattt ccaatgtgca atcataataa gaacatagat
     5161 agaaatgtaa acaccagaag atgatgcagt aattaaggag aaggcatctg aaataccagg
     5221 taaaacactg ataagtgctg aagctccaac gattacagct gatgcaataa tagctcgact
     5281 tggaacgctt tgacgagata attggtctaa ttttaacgct tttgtaagag catttggtga
     5341 ttcattagcc aattggaata aatgacgccc tgttgagtac aaagtagaat taagagcaga
     5401 tgctgcagac gttaggacaa caaagttaat caaagcggca gcccacttaa tcccagctag
     5461 tttgaatact gtaacgaaag gtgactcatt aactggtaac tgttgccatg ggaagatggc
     5521 cataattgct aataatgaac ctgcgtagaa taaaataata cgcatcggaa tttcttggat
     5581 tgcttttggt aaaactttac gtggattagc tgtttcagaa gttgttactc cgacaaattc
     5641 aatagctaaa taagcaaaga ataccatttg gaaagccatg aagaaattca atttaccttt
     5701 agggaaccat tcaaaatgat tggtgatatt tgagattgaa gcatggtatc cgtgaccggt
     5761 gtcaaagttt gtcaatacca taaagatacc ggttgctatt aaaccaagaa ttgcaataac
     5821 ttttatcata gcaaaccaaa actcggtttc tccgaatgct tttacagcaa ttaagttgat
     5881 agaacttagg atagctaaaa aaacaatttg gataatccag gatggccatt cagggaacca
     5941 aaattgaaca tactctccta cggctgtaat ctctgccata ccgataaata ttagggaaat
     6001 ccaataagaa agtgttgaga aaaatcccca accgggtcct aaatatttgg taatgaaatt
     6061 aataaaagta tgctgatttg gatcttgata aagcatctct ccaatagcac gcatcatgaa
     6121 gaacatgaaa gcgccagtga tagcatagac aagaactatt gaaggacccg tcaggctaat
     6181 tgaacgacct gcaccaagaa aaagcccagt tccgattgtc cctgcaatcg caattaactg
     6241 tacgtgacga ttttctaggc cgcgtaccat accattctca gatttttgag tgtggtcatt
     6301 attatttttt gacattataa atactccatc tatttttgaa aaactccttc attatataat
     6361 ttttttgcaa aattcgcaat aattactata caatattttt ctatgataaa atctcctagc
     6421 taagtgagct aagagatttc aattcatccc tcttccttga caatatagac tgggcgtttt
     6481 ttagtttcta ggaaaatctt agagatgtat ttgcctataa ttcctagcga caataactga
     6541 ataccaccca taaaaagtac aatggtaact gtgcttgccc aacctgaaac gggatcccca
     6601 aatagtaatt ttcgaattat aataaaaatg atagctaaaa gtgatagtac tgaactaagg
     6661 gttccaatcc aggtagctat agctaatgga atttctgaga agttgatgaa accgtctaga
     6721 gagtatttta agagattaaa gaaactccat gaggtcttac ccgcaattcg ttctcgattc
     6781 tcataactaa tataagcgac atcatatcca atccaggaaa aaatcccttt tgagaagcga
     6841 ttcacttcat ttagctcgag aatactgtca acaacttgtt ttgtcatgag acgaaaatca
     6901 cgcgcaccat caaccatttt agtatctgac acttgattga ttaatttata aaaaagcttg
     6961 gcaaaaagag agcgtatcaa cggctcccct ttacgatctt ttcgccttgt agcgacaata
     7021 tcataacctt cttgaagttt taggtacatt tctggtaata aagtgggtgg atcttgcaag
     7081 tcaacatcca taacagtgat aaaatcacca gtagtatgtt ctaaaccagc caataaagca
     7141 gcatctttac caaaatggcg tgaaaatgaa aggtaatgaa cattgtcaaa ttgtttagct
     7201 gctttcttaa gaagttcgag tgtacgatcg gtagaaccat cgtttacgaa aatatattca
     7261 aaatgggtgt aacgagccat ttcggattca acttttttga tttctgttaa aaaagttgag
     7321 acagtctctt gttcattata acaagggaca ataactgata ataaagccat ttgtaattct
     7381 ccgtatcatt cttgcttaaa ttttaacata atatgggaaa aaatagaaag attataaggt
     7441 aatcataaaa aggactaggt ttttgcctca gtccttaact taatatgatg agatgttatt
     7501 agtcagcttt aaattgactg agtcgaataa cttcaatttt atagccatct ggatcttgaa
     7561 taaagtagaa catattaggt ttacctggta atccagaaat ttttgttaca gagtaacctg
     7621 ctttctgatg ggcatcataa gtagtttcca aatcgtctac accaacagca atgtgaccat
     7681 acccatttcc taaatcataa gcttcgtgat catagttata ggttaactct aattcgtaac
     7741 tagggtcgtc ctctagggac atatagacta aagtaaattg attttcagga aagtcattac
     7801 gacgaacttc cttaaaacca agtgcttctt gataaaaagc aattgaggcg tccaaatctt
     7861 taacgcgaat acaagtatgt aaaaatggca tagtctctct cctatttctt tattaaacgt
     7921 attatgtcaa aataaaatta tcttttcaaa aatattgatt ataaattagg ctgtctattt
     7981 actcaaccca agtaatgacc tcttgacgtt ctttcctagc acgggaatgt ttaggttcac
     8041 gcaaacgata accaaatgaa accatacaag aaatagcttc tttcttatcg tctattaacc
     8101 cttctttact aagaatatta ttgacctttt catagtcaaa tccttcaatt gggcatgaat
     8161 ctacgccgat catagcagca gccgtcatca tattaccaag tgcgatataa gtttgttttg
     8221 ctgtccaatc ccaaagagaa cgttcagaat caattttcat atcgttttct tggaaggatt
     8281 tgtagagagg tatcctactc tcaagggcgg ctggatcacc tagaccacgt cgaatgagac
     8341 tgttaatcat gctatcagcg ttataataag cacctttttc agctagtagt aaaacaaagt
     8401 gactagcagt atctagttga tattgtgctc cccaagcaac ttccttaagt ttatctcgaa
     8461 attttgcaat tgtttgtcta tctagtacta aaaatcgcca gccttctaaa ccgactgaag
     8521 aaggactcaa ccaagcagtg tctaaaatat actccatatc ttctttagga atatcattat
     8581 tattataaac acgaatcgcc atacgaaaat caaaagctct tcgaacttgt tttttgatat
     8641 cttcttttgt cattttagat cctcctgtta ttaatgctat catatcacaa tttagaaaaa
     8701 atctcagcag gcggtatcgt ggcaaagtag agctacaaaa ttctaatgac tgtcagaaaa
     8761 ataaagggaa tgaacaaaat gatagggttt tgatgaagaa agttatataa ttttacataa
     8821 ggaggggata ataccatata tcaaactatc atttagatgt ttaaaataga tgtttttcac
     8881 tatgaaattc atttttgtca catagaaaag gagattgtta tggaaacata tactctatcg
     8941 aatactctta acattcctaa aattggtttt gggacatggc aactaactga aggtgaagaa
     9001 gcttataaag cagtgactca tgctttaaaa gttggttata ctcatattga tactgctcag
     9061 atttatggta atgaacatag tgttggacgt gctataagag acagtggttt ggcgcgagaa
     9121 agtatctttt tgacaactaa gatttggaat gacaaacatg actatcattt agccaaagct
     9181 tctattgatg aatctctgca gaaattaggt gttgattata ttgatcttct tttgattcat
     9241 tggccaaatc caaaagctct tcgtgaaaat gatgcgtgga aagctgggaa tgcaggcact
     9301 tggaaagcga tggaagaagc ttataaagaa ggaaaggtaa aagctattgg tgtttcgaat
     9361 ttcatgaaac atcacttaga ggctttattt gaaaccgccg aaataaaacc tatggttaat
     9421 caaattatcc tagcaccagg ttgtgctcaa gaagatttgg ttcgcttctg taaaggaaat
     9481 gatatcttat tagaagctta cagtccattt ggaacaggta ctatttttga aaatgaatct
     9541 ataaaagcta ttgctgaaaa atatggtaaa tcagttgcac aagtagctct tcgctggtca
     9601 cttgataatg gctttttacc attacctaag tcagcgactc ccaaaaatat tgaagcaaac
     9661 cttgatattt ttgattttca attaaacgaa gatgatattg ctaccttaat acaactagat
     9721 tcaggtatta aacccaaaga tccagataat gtctcatttt aataagaaaa gatggaaatg
     9781 tatttccatc ttttttatgt gggatagttc cttaaagtta agaaaaacat taagacctcc
     9841 attgacgagg agaatatatt aatgatagta ttactagaaa agtagtaata aaggatgagt
     9901 atggtaaaat cctttacaat atatcattat cgaaagaggt tataacatct aaatgagact
     9961 tgataaattt ttagtagaat gcggacttgg ttcacgtact caagtcaaat taatattgaa
    10021 aaaaaagcag attagtgtta atagtaatag cgagacatct ccaaaagtac aggtagatga
    10081 atatagagat gagattaaat ataatggaac tctagtgtca tatgaaaagt ttgtttatta
    10141 tatgcttcat aaacccaaag gtgttatatc agcaacggat gatccttcgc ataaaaccgt
    10201 attagatttg ctagacaaaa ctgctcgaga taaagctgtt tttccagtgg ggcgtttaga
    10261 tattgataca acaggccttt tgttaataac taataatggg gaattagcgc ataaaatgtt
    10321 atcacctaaa aaacacgttg ataaatgcta tgaggcaaaa atctcaggga taatgacaga
    10381 agacgatatt ttggcatttg ataaagggat tattctgaaa gattttactt gtttaccagc
    10441 attattagaa attgtggaag taaatcaggt gaagaagcag agtcttgtta aaatcactat
    10501 taaggaagga aaattccatc aagttaaacg tatggttgct gcgtgtggca aggaagtctt
    10561 agagctaaag cgtctaagta tgggaaatct acaattagat aaacagctag aaagtggaca
    10621 atggagacgg ctaacaataa aagaaataga aaaattagaa aaacatatgc aataatataa
    10681 aat
//
LOCUS       DATRME010000067        10649 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRME010000067 DATRME010000000
VERSION     DATRME010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(27..1499)
                     /locus_tag="VBC23_001636"
     CDS             complement(27..1499)
                     /locus_tag="VBC23_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843571.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(1730..2566)
                     /locus_tag="VBC23_001637"
     CDS             complement(1730..2566)
                     /locus_tag="VBC23_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6843572.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(2566..3936)
                     /locus_tag="VBC23_001638"
     CDS             complement(2566..3936)
                     /locus_tag="VBC23_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6843573.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4034..5281)
                     /locus_tag="VBC23_001639"
     CDS             complement(4034..5281)
                     /locus_tag="VBC23_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6843574.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            5537..6565
                     /locus_tag="VBC23_001640"
     CDS             5537..6565
                     /locus_tag="VBC23_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6843575.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            6686..8182
                     /gene="malQ"
                     /locus_tag="VBC23_001641"
     CDS             6686..8182
                     /gene="malQ"
                     /locus_tag="VBC23_001641"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6843576.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8194..10458
                     /gene="glgP"
                     /locus_tag="VBC23_001642"
     CDS             8194..10458
                     /gene="glgP"
                     /locus_tag="VBC23_001642"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6843577.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAVKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3635 a         1704 c         1990 g         3320 t
ORIGIN      
        1 tgtaacctag ttctttttag atgtctttat ttaactccag acattagttt ttcaatgcgt
       61 ggaacataaa ataagaggat aaatccaaat acaactgtta taccaccaac aacaccataa
      121 taaagaattt gagtatcagg tgtgtacaat ttaacgattt gagcgttaat agcttgtgct
      181 gcagcattac ttaagaacca gatgctcatc atttgagctt ggaatgcttt aggtgcgagc
      241 ttactagttg ctgataatcc aactggtgag attagcattt ccccaacgat gacaatagcc
      301 caactcattg ttaaccaaag agggcttact ttagcgttaa ctccaaagag taaacctggt
      361 agcatcatcc ataagaatga agctccagca aagaaaagtc catacgcaaa ttttttaggt
      421 gatgaaggtt gcttacttcc ccatttagcc cataaccaag caaagaatgg tacataaagc
      481 ataatgaaga gagggttcat actttggaaa taacttgatg ggaaattaat atgatgccca
      541 aagaagttaa ggtaaagttt tgtttgttca tctgcaaata aggctaagac aactgaacct
      601 tgttcttcaa ttgaccaaaa tagaattgag gcgataaaga gagggatata tgcccacact
      661 cgagaacgct caacagaaga aatcttttta ctacttaaaa tcttgataaa ataaataatt
      721 gggatggcga ttgcaataat actaaaaata tcgataatta cttcaattgt tagcatatgc
      781 atcaagtgaa gtagaataag tacaataaca ataccaataa ggataagccc aactttttta
      841 tatagagagg acttttcttc aggacttaaa ggatcgtttg gtctgagact ctcttcagtc
      901 aaatattttt ttccatctaa ggtatattgg aggagaccaa agaacatacc aatagcagca
      961 agtgagaaac caagatggaa atttacttct tgtccgaggt accccactac aatgggtgaa
     1021 ataaaagcac caaggttaat accaaaaaca aagatactaa aaccagcatc acgacgagaa
     1081 tcattttctc catataaatt accaaccatc tctgatacat ttggttttaa tagtccagta
     1141 ccaaggataa ttagagcaat cgaaatgaat agagctgttt gaccaaatgg tgtagctaga
     1201 gcaatatgac ctagcatgat tagaatacca ccatacagaa cagttttacg actacctaga
     1261 atacggtcgc tgacaaaacc accaattact gatgagagat aaaccagtga accatagaca
     1321 gccatgattg atgcagcgac agtcttgtcc ataccaaggc caccttgaga aacactatag
     1381 tacatatagt acagcaaaat agcacgcata ccatagtatg agaatctttc ccacatttca
     1441 gtaaagaaaa gagtggacaa accacgagga tgtccaaaga atgattttgt tttttccatg
     1501 aaaaacgctc cttttgtaga taacactatt aataatatag taaatagaag caactgtcaa
     1561 attacttagc actatcaagt tatttttttg tagaggttaa cacccttggt aaaccaagtt
     1621 ttttaactat ctagcttaga aaaaggcttg aattcaatag aacccaagcc ttttttagaa
     1681 aagaaaccac agtgaaataa aaatgtcgtg tctttaaatt atctgaagat tattttacag
     1741 atccacctgt aataccttca acatagtatt tttgcatgaa catgaaaagt attgtgattg
     1801 gaattgcaat aataacagat cctgctgtaa atgacatgaa ccactggttg atggtatctt
     1861 gttgcaacat tgaaaagaga ccaatagcga ctgtgtactt gctcgtagca tcacctaaaa
     1921 taacttttgc aaagataaag tccatccaag ggcccataaa agcgattaat gcagtataaa
     1981 cgataatggg ttttgacaat ggtagggtga ttttaaggaa aatatccaaa cgggtcgccc
     2041 catcaatcat agcagactca tccaaagagt atggaatagt atcaaagaaa ccttttgcga
     2101 tgtagaaggt taatgctgct cctgcagaat agacaaatat taaagcggtt aatgtttggt
     2161 ctagatttag agcttttagg atgtagtaaa ctgcaatcat tgacatgaac ccaggaaaca
     2221 tattcaagac taaagctagc tttaggaaac cattacggtg tttgaatttg attctgctga
     2281 gagaataagc cattgctaca gtgattaaag ttgatagtat acaagtacat gtagcgacaa
     2341 ataatgtatt taagaaccat tgaccaaatg ggaatgtgtt ttgggtgaag agtttagcgt
     2401 agttgtctaa tgtccaagtt ttaggaataa agtagttgac aaaggcactt ccttcaccac
     2461 ggaagcttgt gagaacgacc caaacaatgg gaaagagcca cataattgat agtactatca
     2521 ataaaatata aacaaaagta aggttaagac gttttttctt attcattatt tagctgcccc
     2581 ttccttgtaa gaatttgttc ttgtgtaagc tagtaaacta aatactgctg aaagaataaa
     2641 gattaagata ccaacaacag atgctaagtt ataatctgca gcagttactg tcaaattgta
     2701 tagccaagtg acaagtaaat cggtgctacc tgcttggtag tatgttgagt ttgttggtcc
     2761 accttgagtg agtaggtaga taacgttgaa gttgttaata tttccgataa attgttgaat
     2821 taaagtagga gtcataatca acaaaatttg tgggaaagta attgattgaa agacttggaa
     2881 cttattcgcc ccatcaattt cagcagcttc aatttgttct gcaggtaaat tcatgatgat
     2941 accagtagca acaagcattg ttacaggaat accaacccac atattaacaa agatgattga
     3001 gaatttcgcc cagaccggat cagaaaggaa tggaagggga tgtgctgaag aaataagtcc
     3061 ccattttatt aacaaggcgt taaccggtcc agcgtcactt aagaggtttc tcatgattaa
     3121 gagagagata aattgaggga cagccatggt aataacaaaa atagtacgcc acatcttttt
     3181 gaatttcaaa ccttttgtat taattagtaa ggcaagaata ataccaaaga agaaattcgt
     3241 tacagtagcg aaaacagccc aaattaatgt ccaagataga acagggaaga aagtactggc
     3301 catacgcccg ctaaagatat taccgaagtt agcaaaacca acccagtcaa ataaactttt
     3361 tggcggtaag tggttatgat catagttagt aaatgccaaa caaatcatat aaactaacgg
     3421 aaggattgta aatagaagca cccctattaa aggaatagac attaaagtca tatggaagcg
     3481 tccatctgtc aagcttttaa tatctttttt gaaggatgga atttctcttc cttcttcttt
     3541 caaggtcaat aaatgtgctg cgcttttaat gtttgaccag taaatatagg caaatacgca
     3601 acagaagata agcgaagcta atccaaaaat tagcattaac atagagttat caccttgtgt
     3661 cgcaacttgg attttaatac catccacagt tttagttgtc ataccttgtt cttgggtccc
     3721 taaggaaata agacctttta cagctgggat aacttggtaa acaaaggtaa ttaaaaacag
     3781 aatttcactg attaaaaata aaagtccctt gacaatttgt ttatttttga ggttagcaag
     3841 tcccataatt aagaatgata gtttagttgc taaatcacct ttttgaaaga cctcttttag
     3901 ggacatacca tcaagtgaga tcagtttttt attcataatg gtctccttta attgttattt
     3961 tacaaaataa gagatgggaa gcactctcaa ttgaaaggaa gagtactcac ctcatctcat
     4021 tactctagac gtactatttt gttttagcga tgtctttatc gaattgtttt agacgtttaa
     4081 ggtaatcgct agatttgatt ttaccactgt aagtatcgct aagaatagca gcactttctg
     4141 tccagaatgt tgacatttga ctcaacttag gcataaccgt tgtatattta tctgagctac
     4201 ccatttcgat aactgctttt gcaagttcat ctttttggac gctatcagaa gattgaattg
     4261 atgagttagc aggaacgata tgacgttttt cgaattgaat tttttgactt tcagcattag
     4321 ttagatatgc agcgagtttg tagctagctg agattcgttt agtgtttgaa ccagcaggtg
     4381 cttggttaac ggcataaagt ttaacgccca agaatgcttt ttgttgaact tctttgtcac
     4441 cgattttcat tgttgggtaa acagcaacac cgattttatc ttcaccgaca gcttttttag
     4501 cagcgtcgta atcccatggt ccactttcaa aagcatgaac agaaccatcg ccaaatttag
     4561 acattgtatt ttcagctgtc aagttgacaa aaccatcatt tttcttttga tctgcaatcc
     4621 atttaaggac agaaacacct gcttcattac cccagttagt gcctttagca tcttcaccag
     4681 atttaccaaa taaagtgtcg cctacagaaa ggaaaagagg acctgttaca tatgagttag
     4741 ctgctttaag ctgttgaccg aatttccctt tgcttgtaat tgtttcgtat gatttaacgt
     4801 cgtcagcagt taactttgtt ttattataat aaagaacttg agattcaata ccaaatggga
     4861 atgcataagt tttcccttta tattgtgcac cagtaagtga ttgtttagtg tcgtttttag
     4921 caatttcttt tgagtattgc tctggaattt cttggataac accagattct actaattgac
     4981 caagttggtc atgtggaagt gagaatacat cggctgcctt gcttgggtct ttttttacgt
     5041 tttcttgagc tttggagtca ttagactcaa tcatttttac agtaacgcct ttgttttcct
     5101 tctcgaattt tttaacaatt gctttataag acgcttttga atctgttggg acccaaagtt
     5161 tgatagttgt ttttgaagca gcatcaactg agttagagtg agtagcagca attgctcctc
     5221 ctgcaactac tgaaagagca gcagtactaa caagtaattt tttccaagta tttttttcca
     5281 ttttggttct cctcctagag aatattttaa ttacacctat attatgcaac cgtttgcaaa
     5341 aaaagtcaag tcttttttta aaaaaaataa gaaaaataaa aaaataatat caattaatac
     5401 taaatgagaa gattatttta gtgagagtat tacatttgca gagaaggcaa acgtttgttt
     5461 ttttcttaga ttagagtata atagacaaag gactatacca aaaggtataa aatgattaga
     5521 atgaagaggt cgtaggatgg taactataaa agatgttgct gccaaagctg gtgttaatcc
     5581 atcgacagta agtcgtgtac taaaagataa tgaatctatt tcaagtaaga cgaaagagcg
     5641 cgtgaaaaaa gctatggaag agctgggcta tgttcctaat gttgcagcac aaatgttggc
     5701 tagtggttta acgcaaaata ttggagttat ttttcctcca ctggttttac cagaccgctt
     5761 aagtgaaccc tttttcatgg agattctttc tacattaact aatgaagcaa aagagcatgg
     5821 gtttacagtt tctattgcaa caagtatgtc ttctgaggag ttgttagaac aagtaaaatt
     5881 aatgcatcta caaaaacgag tggatggttt tattgttctc tattcggatc aaaaagatcc
     5941 tatcagagag tatttattaa aatataaact gccctttgtt gttgttggtg ctgctaatgg
     6001 ctatgaaaat gagattactt atattgataa tgataatcag ctgatgggga aatctgccat
     6061 tgattatctc tataaaaaag gacataaaag catccagttt gttacagatg atttaaattc
     6121 agaagtatca gaagagcgct atttagggta ttttaaaggt gctagaaagt taggtttaaa
     6181 ccaaaagcca gctcttttat ttgacagagg caatccgcaa gttttagagg aatttataaa
     6241 tagagtcaaa gaagaagaaa caactgcttt aattgttatt ggtgatactg tctctgttag
     6301 agtaatgcaa tttctttctt tctataaatt aaaagttcca gatgacattt ctatcatgac
     6361 ctttaataat tctctcttct cacatctgat tcatccttac ctaagcacat ttgatattaa
     6421 tgttaataat ctaggaagga caagcgttcg tcgtctgatt gacatcatta aatctccaga
     6481 caaagtcttt agtgagacta ttattgtgcc ttttacttta gaagagcgtg aaagtgtccg
     6541 agatatttct aaaaataata attaagctat gatataaaga cttctatcaa ttttcgatag
     6601 aagttttttt aaattatgct tgacttatgc aatcgcttgc gttaagatgg aggagtaaag
     6661 taagtaatcg aaaaggagaa tcaatatgaa aaaacgtgca agtggtgtct taatgcacat
     6721 cacttctcta ccaggtgatt taggtattgg aacatttgga cgagaagcct acgcttttgt
     6781 tgattttttg gtcgaaactg atcaaaaatt ttggcaaatt cttcccttaa caacaacaag
     6841 cttcggggac tctccttacc agtctttttc tgcagtagct ggtaatacac atttaattga
     6901 ttttgatttg ctaacgcttg aaggtttcat ttcgaaagat gactaccaaa atattagttt
     6961 tggacaggat ccagaagttg ttgattatgc tggtctgttt gaaaaacgcc gtccagtttt
     7021 agaaaaagca gttaaaaatt tcttgcaaga agagagagct acgagaatgc tatctgattt
     7081 cttgcaagaa gaaaaatggg taactgattt tgctgaattt atggcgatca aagaacattt
     7141 tggtaataag gcgcttcaag aatgggatga caaggctatt atacgccgcg aagaagaagc
     7201 cttagcagga tatcgtcaaa agcttagtga agtgataaaa tatcatgaag taacgcaata
     7261 tttcttttac aaacaatggt ttgagttaaa agaatatgct aatgataaag ggattcaaat
     7321 tatcggtgat atgccaatct acgtttctgc cgatagtgta gaagtttgga caatgcctga
     7381 actgtttaaa ttagattgcg acaaacaacc acttgctatt gctggtgtac cagcggatga
     7441 tttttctgac gatggtcagc tttggggcaa tcctatttac aattgggatt accacaaaga
     7501 gtcagatttt gactggtgga tttatcgtat tcaatctggt gttaagatgt atgactattt
     7561 gcgtattgat cattttaaag gtttttcaga ttattgggaa attcgaggag attatcaaac
     7621 agctaatgat ggctcatggc aaccagctcc cggtccagaa ttatttgcaa ctatcaaaga
     7681 aaaattaggt gatttaccta ttattgctga gaatttaggc tatattgatg agagagcaga
     7741 gagattactg gctggaacag ggttcccagg tatgaaaatc atggaattcg gtttttatga
     7801 tacaacagga aatagtattg atattcctca taactacaca gaaaatacaa ttgcatatgc
     7861 tggaactcat gataacgaag ttattaatgg ctggtttgaa aatcttacag tcgaacagaa
     7921 agcttatgca gaaaattaca tgcgtcgttt gccaaatgag cccattactg agactgtact
     7981 gcgtaccttg tatgcaaccg ttagtcaaac aacgattaca tgtatgcaag atctactaga
     8041 taaacctgca gatagccgta tgaacatgcc aaataccgtt ggaggtaact ggcaatggcg
     8101 tatgagaaaa gaagacttaa cagaaaatcg caaagcattc ttaaaagaga ttacaactat
     8161 ttataataga gggaataaat aaggaatatt atcatgacaa gaaattttac aacttacgtt
     8221 ggacaacaag gaaaagtatt atcagaatta acaaatgaag agatttacgt tgaattgtta
     8281 aattttgtaa aagaagaagc tgccgctaaa tcaaaaaata gctctcaacg taaggtatac
     8341 tatatttcag ctgagtttct aattggtaaa ttactttcaa ataatttgat taacttaggg
     8401 atttataaag atgtcaaaaa agagttagaa cttgtaggta aatcaattgc tgaaatagaa
     8461 gatgtagagc cagaaccatc acttggtaat ggtggtttag ggcgtttagc atcatgtttt
     8521 attgactcga tttcttcttt aggtattaat ggtgaaggtg ttggtttaaa ctatcactgt
     8581 gggcttttca aacaagtatt tcgcaacaat caacaagaag cagaagctaa ctactggatt
     8641 gagaataatt cttggttagt accaacagat atttcttatg atgttccttt ccgtgacttt
     8701 actttaaaat cacgcctaga tagaattgat gtattaggtt ataagaaaga tactaaaaat
     8761 tatttgaacc tttttgatat tgatggactt gattataacc ttattgaaaa aggtatcact
     8821 tttgataaga cagaaatcaa gaagaattta accttgttct tgtatccaga tgattcagat
     8881 aaaaatggag aattattacg tatctatcag caatatttca tggtctcaaa tgctgctcaa
     8941 ttattaattg atgaagcaat cgagcgtggt tcaaaccttc atgatttagc tgaatatgct
     9001 tatgttcaaa ttaatgacac tcacccatct atggtcatcc ctgagttgat tcgtttattg
     9061 acagaaaaac atggttttga atttgacgaa gctgtgtcag ttgttcgcaa tatggttggc
     9121 tatacaaacc ataccatcct tgcagaagct cttgaaaaat ggcctttaga gtatcttaat
     9181 gaggtagtac cccacctagt aacaatcatt aaaaaattag atcaaatgat tcgtgaagag
     9241 caaacaaatc cagaggttca aattattgat gaagctggac gtgttcacat ggcgcatatg
     9301 gatatccact tctcaacaag cgttaatggt gtagcagcat tacatacaga aatccttaaa
     9361 aatagtgaat taaaggtttt ctatgatatt tacccagata agtttaacaa taagacaaac
     9421 ggtattacat tccgtcgctg gttggagttt gctaaccaag atttagcaga ttacttgaaa
     9481 gaactaattg gggatagcta tcttactgat gcgactcagc tcgaaaaatt gttaacttat
     9541 gctgatagca atgaagttca tgataaatta gctgctgtta aatttaaaaa caaactggct
     9601 cttaaacgtt accttaagga aaataaaggt attgaattgg atgaatactc aattattgat
     9661 acacaaatca aacgattcca cgaatataag cgccaacaaa tgaatgcttt atatgttatt
     9721 cataaatact tagaaattaa acgtgggcat ttcccatcac gtaaattaac agttattttt
     9781 ggtggtaaag ctgctccagc ctatactatt gcacaggata tcattcactt aattctctgc
     9841 ttatcagaat taattaataa tgatccagaa gttaataaat accttaatgt ccatttagta
     9901 gaaaattaca atgtgacagt agcggaaaag ttaatccctg caacagatat ttcggaacaa
     9961 atttcattgg cttccaaaga agcatctggt acaggtaaca tgaaatttat gttgaatggt
    10021 gctcttactc ttggtactat ggatggggca aatgttgaaa tagctgaatt agctggcaaa
    10081 gagaacatct acacatttgg taaagactct gatactatca ttaatcttta tgaaacatct
    10141 ggttatcgtt caaaagatta ctatgataaa gacaaagtaa ttcgtgaagc tgttgatttt
    10201 atcattagtg atgatatagt gtcactcggt aatgcagaac gactaaagag attgcatgat
    10261 gagttagtag gtaaagattg gttcatgaca ttgattgatt tgaaagaata cattgctgtt
    10321 aaagaacaag tgcttgctga ttatgaagat tatgaatcat ggaataagaa agttatacac
    10381 aatattgcta aagcagggtt cttctcatca gatcgtacaa tcgaacaata taatcaagat
    10441 atttggcatt caaattaaaa agtctctaaa agtaaggttt tcaaccttac ttttttcttg
    10501 tctaataaaa ctataagtat ttatttttgt ccgtagactt taaaaataga actgagcaga
    10561 acttattcta aatatttaat tgcatgtgta tttatattct atataatata aaaatatgca
    10621 ctctgtgata cttggttata gggtgtttt
//
LOCUS       DATRME010000068        10633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRME010000068 DATRME010000000
VERSION     DATRME010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            98..1060
                     /locus_tag="VBC23_001643"
     CDS             98..1060
                     /locus_tag="VBC23_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6843578.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            1057..2031
                     /locus_tag="VBC23_001644"
     CDS             1057..2031
                     /locus_tag="VBC23_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6843579.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            2028..2789
                     /locus_tag="VBC23_001645"
     CDS             2028..2789
                     /locus_tag="VBC23_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6843580.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            2851..3879
                     /locus_tag="VBC23_001646"
     CDS             2851..3879
                     /locus_tag="VBC23_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6843581.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            4017..4859
                     /gene="dprA"
                     /locus_tag="VBC23_001647"
     CDS             4017..4859
                     /gene="dprA"
                     /locus_tag="VBC23_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6843582.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            4954..7074
                     /gene="topA"
                     /locus_tag="VBC23_001648"
     CDS             4954..7074
                     /gene="topA"
                     /locus_tag="VBC23_001648"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6843583.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            7211..7912
                     /locus_tag="VBC23_001649"
     CDS             7211..7912
                     /locus_tag="VBC23_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843584.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            7924..10554
                     /locus_tag="VBC23_001650"
     CDS             7924..10554
                     /locus_tag="VBC23_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843585.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
BASE COUNT         3639 a         1770 c         1820 g         3404 t
ORIGIN      
        1 aaaattcttt gaaaaagtag atatttttcc aaaaaattat gttattatag atttgtactc
       61 atttttgaac actttatctt tcattttaag gagttttatg aaaaagctat taaccactcc
      121 tgtaatgggt tgtactttgg ttatactttc tctaacgtcc ctatttgttg gtgttaaatc
      181 tatcccactg gagcaaataa cacaccttaa tcaatcacag gtagatattt ttttagcgag
      241 tcgcttacca agaaccatta gtatcctaat atctggtgca agtttgtcag tctgtggcct
      301 gctaatgcaa caactcaccc aaaacaagtt cgtttcaccg acaacttcag ggactatgga
      361 ttgggcgaaa ttaggagtag ttgttacgct gattttcttt aaaaatacct ctatttttat
      421 tcaactatgt attgcttcag gttttgccat tttaggatcg ctgctttttg taactatttt
      481 aaaaatgatt acttttaagg ataatatttt tatacctctt atcggactga tgcttggaca
      541 aattgtagca gcagcaacag tatttttagg aacacatttt caagtactac aatctgtcaa
      601 cagttggctt caaggtaatt tttccatcat gactagccat cgctacgaaa ttctttactt
      661 agcattgcca tgtttgtttt tagtgtattt ttttgctcat cagtttacca ttgttgggct
      721 tggagaaagc ttcgctaaaa atttagggat tgcttatgaa aagatgattt actttggttt
      781 agttttggtt tcaattatga catccctagt cattattatc gtaggagctt tacctttctt
      841 aggtttaatc gtacctaatc taattagctt aactaaaggg gatcatatgt cctcaactat
      901 cttagagaca tcgcttttag gggcttgtat cgtcatgata tgtgatcttt ttggtcgctt
      961 agttattttt ccttatgaag tctcaatagg agttacgcta ggagtattgg gtagtgcctt
     1021 tttcttgatt tcaattataa gaaatgaggg aaaatcatga ttaaggattt agagatacca
     1081 aaattactta ttttactgat actactaatt gctaccatta ttttgttttt aatttatggt
     1141 attccaactg acgcaaatga atttttgatt atctatatcc taaaaacacg ttatcaaaaa
     1201 ttgattgcat taattttggt tggtatctgc attggtagtt ctagtcttat ttttcaaact
     1261 ctaaccaata accgactttt aactccgtcc attattggtt tagatagctt atatgttctt
     1321 atccaaacag gattaatgta tttaatcgga gctcagcgtg tcataaaatt ttcatctttt
     1381 agcagcttcc tcctttcatt attattaatg gttggattcg cttacttatt atttacgata
     1441 ctttttcgaa ataaaaaaca gtctttgtat tttgttctat tagctggact catatttaat
     1501 acattatttt cttctatctc atcctttatt caagccatta tggaccccaa cgattttatg
     1561 attttacaaa atcagctctt tgctagtttc aatgctatta ataccaagat cctttggatt
     1621 tctttcatta tcattgtcgt tagctttgtc attaattggc cttttataaa ggaattggat
     1681 gtcttactat tgggtaaaga aaatgcgata tcactcggca tctcatatca aaagcttact
     1741 actagaattt ttttatggtt agcattgatg gtagctatag caacagcatt agttgggccg
     1801 attacttttt taggcttact tgtagcacat attacctatc acagttttca tacctttaga
     1861 catcaaatat tagttcctat agccattgtt atctgtatct ttacacttgt cttagggcag
     1921 catctcgttc aaaatctatt acacctcact gttcaactaa gtgttctcct caaccttatc
     1981 gggggaagtt attttatatt caccttaatc aaaggaagga aaaacttatg attcaaataa
     2041 acaatttaca taagttctat ggccaaaagg aaatattaaa agacattaat atttcgattc
     2101 ctaaaggaaa agtaacagct attttaggtc caaatggatc tggaaagagt acattgctga
     2161 gttgtatcag tcgacttgaa ccttatgata atggtgaaat tttccttgat aaagtaccat
     2221 tagcccacta cagttcaaat gacttagcca aaacattagc catattaaga caatctaatc
     2281 acttaacact caagataaag gtgagtgact tgataggatt cggtagattt ccttatagta
     2341 aaggacggtt aagtcaaaaa gataaagctg tcatagaatc agttatatct tatatggatt
     2401 taaacgatat agctgacgag ttcattaata acctcagcgg aggtcaaatt caaagagctt
     2461 ttatcgcaat gacaatggca caagatactc aatatatttg cttagatgag ccactcaaca
     2521 atttagatat gaaatatgct gttcaaatga tggatcttat caaacgatat gcgtatgagt
     2581 ttaataaaac tatagtgatt attattcacg acatcaattt tgcaactcac tacgctgaca
     2641 atgttgtcgc tttaaaagaa ggacaagtgg tgtcttgtgg gactgtcgaa gatgttatgc
     2701 aagaaaaaat cctttctcat ttatttgata tgcctatccg tatcgaaact gttgatggta
     2761 aaccaattat ccttttttgt acaacgtaaa taaagagatt tatgtaggat aacattttta
     2821 gtttttatct attttaggag attttattta atgacaaaaa aacttattat tgctatatta
     2881 gcactatgca ctatcttaac cacttctcaa gctgttttag ctaaagaaaa atcacaaact
     2941 gttaccataa aaaacaacta ttcggtctat attaaaaaag aaaaaagaga caagccggat
     3001 aataaaaagc aaatcagcga gacacttaaa gttcctttaa aacccaaaaa agtagttgtt
     3061 tttgatatgg gagctttgga tactatcaca gctttaggag ctgaaaaatc tgttattggt
     3121 atcccgaagg ctaaaaatgc tctaagttta ttgcccaata acgtcaaatc tgtttataaa
     3181 gctaagagat accaagacgt aggaagtctc ttcgaaccaa actttgaagc tattgctcgt
     3241 atgcaacctg atgtggtttt cctaggagca cgtatggctt ctgttgataa tattgaaaaa
     3301 ttaaaggagg ctgcacctaa agcagcatta gtatatgctg gagtcgactc aaaaaaagta
     3361 tttgacaaag gagttgctga gcgtgtcaca atgttaggga aaatcttcga ccaaaataaa
     3421 aaggcaaaaa cctttaataa agatatcgca caagctgttc ttaaattgca gaaaactatt
     3481 gagaaaaaag gtaaacctac agctctattt gtaatggcaa acagcggtga acttttaact
     3541 caatcacctt ctggtcgttt tggttggatt ttctctgtag gtggatttaa agcagtcaat
     3601 gaaaatgaaa aactaagttc acatggtact cccgtatctt atgaatacat cgctgaaaaa
     3661 aatcctaact atctctttgt tttagatcgt ggagcgacta ttggacaagg agcttcatca
     3721 aaagaacttt ttaataacga tgttattaaa gcaactgatg ctgtcaaaaa caaacgtgtt
     3781 catgaggtag atggaaaaga ttggtatatc aattcaggcg gaagccgagt aacactccgt
     3841 atgattaaag atgtacagaa ctttgttgat aatcgttaat tttttgtcaa aggctaggaa
     3901 aaatcctagt cttatttttt tgaaaaaata gctattatca atgcttcaga gtatagattg
     3961 atcttatcac tttattttgt ctattatttt cgaatatagt caatggaggt aaccttatga
     4021 atcattttga actatttaag cttaaaaaag ctggactaac aaatcttaat atcaacaata
     4081 ttatcaacta tctcaaaaag aatagtttaa cttctctatc tgttcgcaat atggccgtag
     4141 tatcaaaatg taaaaatcct actttcttta tagaaaatta taaacagcta gaccttaaaa
     4201 aacttcgaca agaattcaaa aaatttccag ttctatcgat tttggattct aactatcctt
     4261 tagagttaaa agaaatatat aatccaccag ttctactttt ttatcagggt aatattgaac
     4321 ttctatctaa acctaaatta gctgtagtgg gggcaagaca ggcatctcag ataggttgtc
     4381 agtctgttaa aaagattatc aaggagacta acaatcaatt tgttatcgta agtggtttag
     4441 cgcgtggcat tgatacagca gcacatgtta gtgctttaaa aaatggcggc agtagtatag
     4501 ctgttatcgg gagtggttta gatgtttatt atccaacgga gaataagaaa cttcaagaat
     4561 atatgtcata taatcatctc gtattatcag aatattttac cggagaacaa cccttgaaat
     4621 ttcatttccc cgaacgtaac cgtattattg cagggctgtg tcaaggtatt gtggttgccg
     4681 aagctaagat gagatctgga agtttaatta cctgtgaaag agcattagaa gagggacgag
     4741 aagtttttgc cattcccgga aatattatcg atggcaaatc agatggatgc caccatctta
     4801 tccaagaagg agctaaatgc attatttcag gaaaagatat cctttctgaa tatcagtagt
     4861 gttttgcttt ctactatagg agaagttttc tattgacagt ttaagaaaag tgagttatta
     4921 tgctataagt ttacgatata gaaagtgata cctatggcaa ctacgacaaa aacgtcgacc
     4981 aaaaaaacaa gtaaaaaaaa atcagctact gctaaaaaaa atctagttat tgtggagtct
     5041 cctgcaaaag caaagactat cgaaaaatac ttaggacgta actataaagt tgtagcctca
     5101 gtaggtcata tccgtgattt aaaaaagtca agtatgtcca ttgattttga gaataattac
     5161 gaaccacaat atattaatat tcgtggtaaa ggacctctca ttaatgattt aaaaaaagaa
     5221 gctaaaaagg ctaaaaaagt ttacctcgcg agtgacccgg accgtgaagg agaagctatt
     5281 tcctggcatt tagcgcatat tttagattta gacaaagaag acagaaaccg tgttgttttc
     5341 aatgaaatca caaaagatgc tgttaaaaat gcttttgtgg aacctcgtca gattaacatg
     5401 gatctcgttg atgctcagca agcacgacgt gttttagatc gcattgttgg ttattctatc
     5461 tcacctattt tatggaaaaa agtaaaaaaa gggctatcag ctggacgtgt acaatcagtt
     5521 gctcttaaac taatcattga tcgtgaaaat gagattaagg ctttccaacc ggaggagtat
     5581 tggactattg acggttcctt taaaaaagga acacgtaaat tcaatgccac tttctacggt
     5641 ttagacggaa agaaattcaa attatcgaat aatgaagacg tcaaaacagt tctaaaacgt
     5701 attaaaactg atgaattctt agttgaaaaa gttgaaaaaa aagagcgtcg tcgtaatgca
     5761 ccattaccgt atacaacttc ttcattgcaa caagatgcag ctaataaaat caattttcga
     5821 actcgtaaaa ccatgatgat tgcgcaacag ctgtacgaag gacttagctt aggcacagca
     5881 ggtcatcaag gtctgattac ctatatgcgt actgattcta cacgtattag tccgttagca
     5941 caaaatgaag caacagaatt tattactaac cgttttggtg caaattattc taaacatggg
     6001 aataaagtta aaaatgcttc tggagcacaa gacgctcacg aagctattcg cccatctagt
     6061 gttaatcata cacccgaaag tattgctaag tatttagaca aagatcaact aaaactttac
     6121 actcttatct ggaaccgttt tattgcaagc caaatgacag cagcagtctt tgacacaatg
     6181 aaagttaatt taacgcaaaa tggtgttact tttattgcta atggtagcca agtcaagttt
     6241 gatggttaca tggctgttta taacgatact gacaaaaata aaatgttacc agatatggag
     6301 gaaggagaaa gtgttaaaaa ggttaatacg aatcctgaac aacacttcac tcaacctcct
     6361 gcaaggtttt cagaagcaag tctcattaaa acacttgaag aaaacggtgt aggtcgtcct
     6421 tcaacttatg ccccaacgct tgagactatt caaaaacgtt attatgttaa gcttgcagct
     6481 aaacgcttcg aaccaactga acttggtgaa attgttaata gccttattgt agaatttttc
     6541 ccagatattg ttgatgttac cttcactgcc gaaatggaag ggaaactaga cgaagttgag
     6601 attggtaaag agcagtggca aaagattatc gatgaatttt ataaaccatt tgaaaaagaa
     6661 cttgcaaaag cagaaactga aatggagaaa attcaaataa aggatgaacc tgctggattt
     6721 gattgcgagc tatgtggatc accaatggta ataaaactag gacgttatgg aaagttttat
     6781 gcgtgtagca atttccctga atgtcataac acaaaagcta tcactaagga aataggtgtt
     6841 atttgtccta tctgtcaaaa aggacaagtt attgagcgaa aaacaaaacg caatcgtatc
     6901 ttttatggct gtgaccgcta cccagaatgt gagtttacat cttgggacaa acctattgga
     6961 cgaacttgcc ccaaatcaaa tgatttctta gtggagaaaa aagtacgcgg tggtggtaaa
     7021 caagttgttt gctcaaatga aaaatgcgac taccaagaag agaaaattaa ataaagtgga
     7081 atctgagtgt ttagctcaga tttttaaaaa aattattatt ttacataact taatttatgt
     7141 atttattttt atgggagaat taataaaaaa gtgctataat ataggtgtat tcatagtgtg
     7201 aggtgccgtt atgtcctata ttgaaatgaa gcattcctat aagcgttatc aaacaggtga
     7261 aaccgaaatt gttgctaaca atgatattag tttcagtatt gaaagaggtg agttggtggt
     7321 tattcttggt gcatccggcg ctggcaaatc tactgttctc aatatactag gagggatgga
     7381 tagtaatagt gaaggtgaag ttttaattga tggtaagaat attgctaatt ataccatcag
     7441 ggaattgact cgttaccgta gatatgatgt cgggtttgtt tttcaattct ataacttagt
     7501 ccctaatcta acagcactag agaatgtaga attggcttca gaaattgttc caaaggcatt
     7561 agatgcccaa caggcattag aaaacgtagg tttgggacac cgtatcaatc attttccagc
     7621 acagctttcc ggtggcgaac agcaacgcgt tgcaatagca cgagctattg ccaaaaaacc
     7681 taaactgtta ctttgtgatg aacctactgg tgctttagat taccaaactg gaaaacaagt
     7741 actagccatc ctgcaaaaga tggcgcaatc aaaagaaaca actgtgatca ttgttactca
     7801 taatactgcc ttagcgccaa ttgcaaatcg cgtgatccat atgcatgatt caaaaatcag
     7861 tgatattgta ataaacgaga atccaagtga tattcagaac atagaatatt agaggttata
     7921 cagatgggaa agacattttg gaaagatatc tatcgatcaa ttacgacttc aaaaggtaga
     7981 ttttcatcaa tcctcttact tatgatgtta ggctcatttg cttttatagg actgaaggtc
     8041 tcagcaccta atatgcaaag aacagcacaa aattaccttg ctcatcatca tgttatggat
     8101 attaccgttt tcaattcttg gggacttgac aaacatgatc aaactgtttt agaaagctta
     8161 aaaggatcac aagttgaatt ttcttacttt gtagatacaa cccctcaaca gaatagtaaa
     8221 tcttatcgat tgtattccaa tactaaaacg atatcaacct ttgatcttgt taagggacgt
     8281 ctccctttaa ataaatcaga aattgctctc tcatttcagg aacgaaaaaa atatgcaata
     8341 ggtgataaaa ttaattttaa acaagataaa aacaaactgt tttcaaatac aggtcctcta
     8401 actattgtag gttttgttaa ttctaccgag atttggtcta agacaaacct tggtagttct
     8461 caaacagggg acggcgactt agatagttat ggcgtattag ataagacagc atttcactct
     8521 ccagtatata caatggcaag ggtaactttt aaagatctta ggttgattaa ccctttctca
     8581 ataagttaca aagaaaaagt agcgaaatac caagaaaagg tatcgcgaaa attaaatatt
     8641 cacaataaaa taagatatac caaaacaaaa aaagagagcc tacgtaaaat cgatgaggag
     8701 gaaaaaagcc tactaaaagc tcaaaaacaa atcaatcggt tagataatga tagcctagcg
     8761 atgccactat ctcaaagaca agctattcaa atgaaaatta aacaagaccg actatctctt
     8821 ttgaaacgaa caaaagagct tctaaaattg agacataaca cccaaataat ggaatcgcct
     8881 caaattattg tctacaaccg tactaccttt cctggaggac aaggatataa cacttttgac
     8941 tccagtacaa atagcacttc taaaatcagt aatcttttcc ccattatttt atatttagtc
     9001 gcagcattag taaccttaac aacaatgact aggttcgtcg aggaagaaag aaccaacgca
     9061 ggcatattaa aagcactagg ctatagtgac cgccaagtta tctttaaatt tattatttat
     9121 ggttttatag caggaacatt agggactact ttgggtatca ttggagggca ttatctttta
     9181 cctcgtatta tttctgatat catttccaaa gacctgacta ttccaaacac acagtatcat
     9241 cttttcttaa attacagttt actagccttt gttttttcat tattaagcat tgttcttcca
     9301 gtttttgtta tcactcgacg tgagttgaaa gaaaaagcag catttttatt attaccaaaa
     9361 ccaccagcta aaggatctaa aatagcttta gagtatatca attggatttg gaaaaaactg
     9421 tccttcacac aaaaagtaac tgctagaaat atttttcgtt acaaacaaag gatgattatg
     9481 accatatttg gagttgctgg atcagtagcg ctcttatttt caggtctagg aatacaatcc
     9541 tccttaaagc aaaccgtcaa tgaacatttc ggtcgtatta tgccctatga tatattgcta
     9601 acatataaca caaatgcttc tcccccaaaa atacttgaac tactttcaaa agattcaaaa
     9661 atagacaaat accaaccaat acaccttgaa aatcttgacg aatccattcc tggacaaatt
     9721 aataaacagt caatttccct attcataaca gacaaaaaac aattactacc ttttatttat
     9781 ttacaagaag caacaactaa taagtcattg cacttgaata ataaagggat tattatttct
     9841 aaaaaattag ctcaatttta tcatgttaat accggtgatt ttatccattt atctcattca
     9901 caaacacttc cttctagaaa attaaaaata acaggagttg tcaatgcgaa tgttggtcac
     9961 tatattttta tgacaaaaca atactatcga actattttta agaaagaagc taaagataac
    10021 gcttttttgg ttaagttaac caaacataaa atcgcaaata acttagcaga aaaactttta
    10081 gaaattaatg gagttgagtc tcttacgcaa aatgctcttc agctggctag tgtagaagcc
    10141 gttgtgcgtt ctttagatgg ctctatgact attttagttg tcgtatcttt attgttagcc
    10201 attgtaattc tctataatct cactaatatt aatttagcag aacgtaagcg ggagctatca
    10261 actataaagg tattaggttt ttataatgaa gaagtaactt tatacattta ccgcgaaacc
    10321 attattttat caaccatcgg tgtgattcta ggcaccatta gcggtactta tttacaccgt
    10381 caaatgatgt tgctaattgg ttcagatcaa atactttttg gtgaaaaagt atcaccaact
    10441 acatttataa taccaataag cgtagtagtc atcattctaa taagtctagg ttttatagtt
    10501 aaccatcaat taaaaaaact caatatgtta gatgccttga aatcagtaga ttagtttata
    10561 aaaaagatta tcttttctaa tcttttcgat aacgattgac agcaagtaat cttaattgta
    10621 taatataaca gta
//
LOCUS       DATRME010000069         9962 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRME010000069 DATRME010000000
VERSION     DATRME010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(100..2004)
                     /locus_tag="VBC23_001651"
     CDS             complement(100..2004)
                     /locus_tag="VBC23_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6843586.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            complement(2084..2899)
                     /locus_tag="VBC23_001652"
     CDS             complement(2084..2899)
                     /locus_tag="VBC23_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6843587.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(2886..3752)
                     /locus_tag="VBC23_001653"
     CDS             complement(2886..3752)
                     /locus_tag="VBC23_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6843588.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            complement(3788..4282)
                     /locus_tag="VBC23_001654"
     CDS             complement(3788..4282)
                     /locus_tag="VBC23_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO6843589.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            complement(4337..5533)
                     /locus_tag="VBC23_001655"
     CDS             complement(4337..5533)
                     /locus_tag="VBC23_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO6843590.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(5536..5970)
                     /locus_tag="VBC23_001656"
     CDS             complement(5536..5970)
                     /locus_tag="VBC23_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6843591.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            6248..7060
                     /gene="dhuD"
                     /locus_tag="VBC23_001657"
     CDS             6248..7060
                     /gene="dhuD"
                     /locus_tag="VBC23_001657"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6843592.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            7077..7715
                     /gene="dhuI"
                     /locus_tag="VBC23_001658"
     CDS             7077..7715
                     /gene="dhuI"
                     /locus_tag="VBC23_001658"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6843593.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            7741..8748
                     /locus_tag="VBC23_001659"
     CDS             7741..8748
                     /locus_tag="VBC23_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6843594.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            8760..9398
                     /locus_tag="VBC23_001660"
     CDS             8760..9398
                     /locus_tag="VBC23_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6843595.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         3188 a         1927 c         1643 g         3204 t
ORIGIN      
        1 atggtcattt ttttagacat aacaaacctc ctttctaatt ttactgattt tattttttta
       61 tggttaatat agtactagta tagcataata atagccctct taatttttta aacgactcat
      121 tttgccatta tttttatcat atacaatgac ttttcctttg cattttatcc cttgcatcaa
      181 atagagttta tccccaaaga taatatcatc catatgcact ccaattaaat aatgaaagtc
      241 ttgagaccta atatcaaatg caatgctatt acttagttcc ttatgactgt tcgtttgcac
      301 taaaggaaca catttcacct gacagttccg atcaactatc aaggtataat ccatcacctc
      361 atcaacaaat tttattttct taactaattt atgactttct gtcaattgat tataccgctt
      421 agaaataagg caatcttcta gattaaaggt cgtaggactt actaatttca agtcgttaac
      481 aagcccattt tcataaatga cctgatcatc taaaatatat tgatttgtta aaacatgctc
      541 tcctggacaa ttcaccttat caataactaa ggtaatggcc tgcggtaaag ttaacaacca
      601 tcgcttatgg taatagatat cagaagctct ttggacatgt agttgtcctt cgactaaatg
      661 gtacctatcc tttgaagtca gctgacaata gtcacatttt ggataagaat tgtatgtcca
      721 agagtccttg atcatttcta aagattgccc atcaagggta catgttgaat gactagtcga
      781 acgtttaaaa tcatacctta gttgttcttc tttgtaagta tatcttcctg catcaatgaa
      841 aataggtttc tttttatcat agagacaaac actattatta tcactatgag tatgagcgct
      901 accaaatgga ccgttcttaa aaaatatata acgcctatca tcacgtaaac acacatgacc
      961 agaatctgga aaaagataag cagactcacc tattgttgca cgcggtattt cttcaaaaag
     1021 ataaattgat ggcttcccaa acaaaagtaa agtttctaaa ttaacattat caaaagaaaa
     1081 tgattttgtt ttagaagatt tcaacacaag tgttgccaaa gttagaatat cacgagtatc
     1141 ggtcacatca ctgtccccta tagctagttg acaataatcc ggaccagtca tagcaattag
     1201 ataggtaacc attttttcaa tagtttcttc taatggtaga taatatttag gcgcaaggat
     1261 aactagttcc attaaggact tcaagacttc tacatgatac ataattgatt gttcatactg
     1321 gctcccgtca tctaaaattt gaagcttgat ttgaagtaat aattcttctt cagcaaaact
     1381 ctgaatttca ggtaggttta attcatcttc ataatagtat aaacacgcca atattgctgt
     1441 tgtttgtaaa attccccagt tacttagact atctttttca cgataatagt ccctcatata
     1501 agttatctgc tcccgtaaac tagttaatag tttaattttt ttagtttctg ttattaatcc
     1561 aaaataatct aaaaaaatca aaactttgag ccatgacatg caacgtatcc cagtatcaat
     1621 agtacgactt accgcccctt ctggctttag tgtaaattgg caatcaatcc aatggtacat
     1681 aaaatatttc atttgtctaa gataagactt atcgccttca acaatgtaga ccaccaggaa
     1741 tttaaaaaga taagtttgac gatttagcat atacaaccat tctggatcat ctgttactgg
     1801 attatcccac tccaaaggat caagacaata tgctttagaa caaggttcca tatcccaatt
     1861 atcattaaat acaaaagtgt tcctcatcat caaattaaca gaggctttca tttcttgata
     1921 ggcattagat tggtatgttt taatatactt ttgacaaaat tccctatcaa aatccttgaa
     1981 tttattatat aagcgatctt tcattttttt acaaaacgtc ctcgtccgat ataaaaagga
     2041 ctatgtcatt gatatatcat aatccttatt tatcaaaatt ttattaagct aaaataccta
     2101 accagctacc aaggatacca ataataaccg taaggataac tagcttataa gttgtccatt
     2161 ttttattttt aataaggtaa aacataagga aagtgtagag tgctggaagt agagctggtg
     2221 caattttatc aagcatacct tggacagtaa caattttttg cgctgtacta gttacttttt
     2281 ctggagcaaa agtgtaaggg attgtaagct taacttgtgt tgcagctaaa cttgagatga
     2341 ccgttacacc aacgatatta gctgcccgtg agataactga catttgttca ctcaatttat
     2401 caataaaact ggtcccaaga cgataaccat acatgccagt aactaattta attcccgtta
     2461 aaatagtatt catagccaca aagaacagaa tcggtcccat caccaaacca tctgtcgcta
     2521 aagaggctgc aatcgttgag aacaagggtg ctaagcaaaa ttgtgataaa gaatcaccga
     2581 tacctgcaag aggacccata agcgccatct taataccgcg aatttcttct tctggccgct
     2641 cattatcaag cattgctaaa tgtaaactag ttacaaaagg taagaaatga aggtttgtat
     2701 tgtaaaactc cacattttct tctagagctc ccgctaaagc ttttttgtca tctccataat
     2761 attttttgag agctggatat atgacattag cataaccaag accttgatag ttactgtaat
     2821 taaagccatt ttgtaaataa aaggcacgta aagctgtttt agtgtaatca ctttttgcta
     2881 attttttaga tccagtcatc ttggttgtct ccttctgtct tagtttcttt agtaggtgca
     2941 gctggttttc tgaaaatatc gagcaaagca aatattgttg caattatagc taccccgata
     3001 gttggaaccc caagcacact gttagtagcc actgatgtta aaactccgtc tccatttgct
     3061 gttggagtta gaactggcaa accaaaataa acagctagaa catatccaag taggatataa
     3121 ggaatgagtt cttttttagc cattacagag agaatcatcg caaaaccaat tgcaggaagc
     3181 attttcccag ctaaggttag accattaagc aaaacagggg gaatgagagc aaataaatct
     3241 gtcaaagttt gcgttgaaag agcacccagt actcctaaac caaatcccaa tccagcgaat
     3301 gcccaaattg taccatttgc tgcaatttta aatcctcgga aattacctgc ttgcaaagct
     3361 tttttagctg tttcaggcgc acctgcaaaa gcagtataag ttgctgtttg aagaaattga
     3421 ataccaactg caattggggt tgataatgca agagcagctt ccggtgtgat ttttccttta
     3481 gttccagctg ttgtaatagc catcaaagtt ccaaaaatcc caggaccgat tgggtttgga
     3541 ggaactgtcc ctcccgctcc cacaccaaat cccatgtaag ccagttcaga tattgctcca
     3601 actgccaaag ctgtttgaat atctcctaaa ataacaccaa cgcctagaga gagaacgata
     3661 caacgatttg tatagaggcc aagcaacatg ccactaaaac agaaagccgt ccacagtcca
     3721 attaagacag cctgaagaat actaatatcc atatttttct cctttatatg atagtgagtt
     3781 tgtccaacta aatataatct aaaatattaa cttccacagc accatcgtta cctgttggag
     3841 ttgttttagt attaaatgca atgtgatatt cttggtttaa ttttctaatt atctctttat
     3901 ccttcatccc taagaagata gagcgactta cctgctcttt accttctcca ttatgaatat
     3961 ttccaatatt aatctcttta attgggacgc ctcctgctac aagtctatag acatctttca
     4021 aatctttaac aattataaaa atcgtctgtg ctgggttggc cttgtgaata atatcaataa
     4081 ctttttgaat atcaaaaaat cgcaatgcaa ttgattctgg caccaccgtc ttcattaacg
     4141 tttgttgaag atgatctttg gagacatcat catttgccac aataacagta ttacaactta
     4201 aaaatttcac ccaaagttgt ccttgtccat gaataagacg ctcatcaaca cgtgtcatta
     4261 caatatttgg tcctgctgcc atttctgacc tccttgtttt ctattattta ttgtttattt
     4321 tgttagtaaa atactcttac caataaagtt cccagtcttt atagaatctt attaaggctt
     4381 ctaagtaata ataatctccc caaatattac cttcatctac tcctttacct gaatgccacg
     4441 aatacacacc gtgcaataga agaggacgtc ctgctataaa ttcactatta ctatactgtt
     4501 caatcagact acgaagcatt gtatgcatag catatttgta tgtctcttta tcaggatcta
     4561 cttctggtaa atgtttaagc atctcatgaa tcccacacac agctgttgct gttgcggatg
     4621 tatctctagg ctgacccgag ccatccgtaa aaataaggtc ccaataggat accttgtctt
     4681 caggtaaacg atttagaaaa tagtttgtca tacctttaaa aaggataatc tgctgataat
     4741 ctttcatttt ccggtaacta agcggaatac cgtagatgcc ccatgcttgc cctcttgccc
     4801 aagatgactc atcactataa ccctgtcgtg tgacaccttt taacggttct ccagtttctg
     4861 ggtcgaaata aaaagtatga aaagcagaag aatcatcacg caccacattg ttagctgaag
     4921 cgtagaagtg attcaccgca acttgtctat acttttcatc acctgtctgt tcataagcaa
     4981 aaaataagag ttggatatta agtaagcaat cgataattaa gcgatagtgt tccttatacc
     5041 cgagttctcc ccaagcctga ataaatccac ctttttcttg atagcgctcc atcaatttat
     5101 ctgcagcttt tatagtggct tctaaagctt tagcatcacc attgatacga tattctgctg
     5161 tacaagatgg tgtgtaaaga aatcctaagt cgtggtgatc taatgctata cgattattaa
     5221 tacgatttag aaatgacaat acatttttgt gggctatgtt ttttaacttt ttatcctgat
     5281 tatactcata agctaaccac aagcaccctg tccaaaaacc atttgtccat tctgtgttat
     5341 ccataacttt ataagtatta ttgaatgttg caggcgttgg atactcttca ccaaagtaat
     5401 caatattaat ataaagttgc ttcaaggctt tttcaatggc ttcctcaact tcaatcttag
     5461 ttaataatct actgtctaaa aaacgctttg gattttcaat tgactcaacc ttgaccggtt
     5521 ttattttcat cattattaaa tgcctccctc aaaagttgct tctgcagttt gtgtagcaag
     5581 gcaagtagaa aaatcaacaa ttccttcatg agatgatgtt ataactttat caacaaggtc
     5641 atctacatgt tcaaacatcg ttctagataa aacagcttcc attaacattg aaaggttcaa
     5701 gcccgataaa acttttatct ttttatctgt atattctact gataaggcgg aagaaacatt
     5761 gaaaggagtc ccacctagca aatcagtcaa tactaaaatt tctttgaaat cgataacctc
     5821 tctttgcaaa gcaactctaa catcattaga agacatgcct gcaataaagt taataccaac
     5881 aacatattcc tgatgcccag caataagttc taaagagctt aaaataccat ctggaaagtt
     5941 gccgtgtgct acaataataa tttttatcat attttctcct cttgagttta ccggtttact
     6001 tttataatca tattatagcg ctttcatttt taggtgtcaa catctttttt atattttttg
     6061 agcagtttac ttgatttttt ataatgatgt cttttttatc tttaagaatc ataacttaga
     6121 taaagctttg ttatatatgt tgccaagttt tataggagaa gaactctata gttgacaaaa
     6181 tcggtttact ttgttactat tcatgtaaac aggtttactt cctgaatttt tttacggagg
     6241 tcatattatg actgaacaat tcttaaaaga caacttttca ctagaaggta aagtcgcctt
     6301 aataacaggc gcttcttacg gcattggttt ttcaatagca actgcttttg ctagagctgg
     6361 agcaactatt gtcttcaatg atattaagca ggaactcgtt gacaaaggca tctccgccta
     6421 taaaaaactt ggcattaagg cccatggcta tgtttgcgat gttacagatg aagatggaat
     6481 caacgaaatg gtgaacaaaa tttctcaaga tgttggggta attgatattc tagttaacaa
     6541 cgcaggtatt ataaaacgca cccctatgct agagatgagc gctgcagatt ttcgacaagt
     6601 tattgatatt gatttgaatg ctccctttat cgtatcaaaa gcggtattac ctggaatgat
     6661 ccaaaaagga catggtaaaa tcatcaatat ttgctccatg atgagtgagc ttggacgcga
     6721 aacagttgct gcctatgctg ctgccaaagg gggacttaaa atgttaacca agaacattgc
     6781 ttctgaatac ggttctgcta acatccaatg taacggtatc gggcccggct atattgccac
     6841 accacaaaca gctccccttc gtgaacgtca agatgatggt tcacgtcatc cattcgacca
     6901 atttatcatc gctaaaacac ctgccgctcg ctggggtgag gcagaagatt taggtgcacc
     6961 agctatcttc cttgctagcg atgcatcaaa ctttatcaat ggtcacatac tctatgtaga
     7021 tggcggtatt ttagcttaca tcggtaaaca acctcaataa taaaggagta atataaatga
     7081 aaattgcatt aatcaacgaa aatagccaag cgagtaaaaa tactcttatc tacaaagaat
     7141 tgaaagcggt ttccaaaaaa aaaggatttg atgttttcaa ctacgggatg tatggcaaag
     7201 aagaagaaag ccagctaact tatgtacaaa atggtttact gactgctatt ttacttaata
     7261 gcggtgctgc agattttgtt attacaggct gtgggacagg aattggagcg atgcttgctt
     7321 gtaatagctt tcctggcgta gtctgtggat tcgctgctga ttctgtcgac gcttacttat
     7381 tctctcaagt aaacggagga aatgctcttt cccttccttt tgctaaagga tttggttggg
     7441 gagctgaatt gaatctgagg tatttatttg aacgtctttt cgaagatgaa aaaggagggg
     7501 gataccctaa agagagggct gtacctgagc aacgcaatgc acgtatcctt agcgaagtaa
     7561 aacagattac ttacagagat ttactctctg ttttaaaaga aattgatcaa gatttcttaa
     7621 aggaaactat ttctggggag catttccaag aatatttctt cgctaattgc caaaatcaaa
     7681 atatagcaga ctacttgaaa tctgtcttag actaaagcgt ctgttttaga aaggactttt
     7741 atgtctaaga ttcttttttt cggtgaacct ctcattcgca tcactcccaa agaaaatgac
     7801 tattttgcag atagtatctc aactaaacta ttctatggtg gctctgaagt taatatcgca
     7861 agagctctac aaggctttgg gcaagatact aaactcctct cagccttacc caataatccc
     7921 ataggtaata gttttcttca atttttaaaa gcacagggta ttgatactca ttctattcag
     7981 tgggttggag aacgtgttgg gttatatttc ttagaaaatt cctttgcttg cagaaaaggt
     8041 gaggtggtct acgaccgcga ttatagtagt ttacatgact ttaggataaa ccaaattgat
     8101 ttcgatcaat tatttgaagg agtttctctc tttcatttta gtggtattac actatcattg
     8161 gatgagtcta tccaagagat aacactgctt cttttaaaag aagctaaaaa aagagagata
     8221 acaatctcct tagatctcaa ttttcgcagt aaactaattt ctcctaaaaa tgccaaaatc
     8281 cttttttctc agttcgctac ctttgcggat atttgttttg gtattgaacc tcttatggtt
     8341 gactcacagg acactacctt cttcaatcgc gatgaagcta caatagagga catcaaagaa
     8401 cgaatgattt ctcttataaa tcactttgac tttcaagtca ttttccatac taaacgtcta
     8461 caggatgaat ggggacgaaa ccactaccaa gcttacatag caaatcgtaa acaagagttt
     8521 gtcacttcaa aagaaatcac cacagctgta aaccagagaa ttggaagcgg agatgctttt
     8581 gtggcgggag cgctatatca actacttcag cactcagaca gcaagacagt cattgatttc
     8641 gctgtcgcta gtgcatcttt aaaatgtgct ttggaagggg acaatatgtt tgagaccgtt
     8701 accgcagtta acaaagtact aaatcaatcg aaagacatta ttcgttagaa gggagaatta
     8761 tgataaaatc aaagattttg ttgcaattaa tgcaaggaaa aatagttgct gtcattcgtg
     8821 gaaatagcca agaagaagct tttcaagctg ctcaagcatg cataaaaggt ggtatttctg
     8881 ctatcgaaat cgcctatact aatagcaaag ctagccaagt cattgaacaa ctagtcacac
     8941 aatatactaa ccaagaacaa gttgtcgttg gggcaggaac tgttctagac gcagaaactg
     9001 cccgaatggc tattttagca ggagcaaagt ttattgtatc tcccgcattt aatctacaga
     9061 ctgctaaatt atgtaatcgt tatgctattc cttatttacc aggttgcatg accttatcag
     9121 agataacaac agcacttgaa gctggttgtg aaataattaa aatattccca ggaggtactc
     9181 taggaacatc tttcatctca tcactaaaag cacctttacc tcaagtacaa attatggtga
     9241 ctggtggggt aaatctcaca aatgccaaag actggtttct atctggagta acagccattg
     9301 gtattggtgg agagttcaat aaactagcag ctttggggga atttgataaa atcacagaaa
     9361 tggctaaaca atacaaaata cagctaaatc tgaattaaca caataaaaag acaaggcacc
     9421 cccacacctc aatcatgaga cagaagaaaa gaacttttat aaagccattt ttctgtagta
     9481 ccgtttatct taatcagact cattatctcg attttcttat cagaccgaga taatttttag
     9541 ctaatcttcc tgtagacata accgttctac ttccgacaac tgatccatgt cttcaccaaa
     9601 gatatactac ttgcccgcag attgatataa gtagtgaagc tcatcattct catatcattt
     9661 cagcaaataa tagatttgat atcattttta tatccaaaaa cttagcaatg acctatcagg
     9721 cttacccgct tttcctatct caacacaggc taacttagct cctatcaaat acatttttta
     9781 aacttaatta aaaacatttc tgattatagt ttattagatt tcaacagaag tatttttcct
     9841 gcgtctcagc ttttgggtgt accgttattt tgaagttttg atagttgagt gatatcatta
     9901 atagtcattt tttagacatt ctaaacctcc tttctaattt gaccgatttt attttttata
     9961 ac
//
LOCUS       DATRME010000070         9812 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRME010000070 DATRME010000000
VERSION     DATRME010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1319)
                     /locus_tag="VBC23_001661"
     CDS             complement(<1..1319)
                     /locus_tag="VBC23_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6843596.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESM"
     regulatory      1627..1759
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1916..3262
                     /locus_tag="VBC23_001662"
     CDS             1916..3262
                     /locus_tag="VBC23_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6843597.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     gene            3326..4561
                     /locus_tag="VBC23_001663"
     CDS             3326..4561
                     /locus_tag="VBC23_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6843598.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            4720..5112
                     /locus_tag="VBC23_001664"
     CDS             4720..5112
                     /locus_tag="VBC23_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6843599.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPIPGSIIGLILLFLALKY
                     KIIRLRHIDTVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            5093..5788
                     /locus_tag="VBC23_001665"
     CDS             5093..5788
                     /locus_tag="VBC23_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6843600.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            5857..6480
                     /locus_tag="VBC23_001666"
     CDS             5857..6480
                     /locus_tag="VBC23_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6843601.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(6749..6820)
                     /locus_tag="VBC23_001667"
     tRNA            complement(6749..6820)
                     /locus_tag="VBC23_001667"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6785..6787),aa:Arg,seq:ccg)
     gene            complement(6893..8095)
                     /gene="rpsA"
                     /locus_tag="VBC23_001668"
     CDS             complement(6893..8095)
                     /gene="rpsA"
                     /locus_tag="VBC23_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6843602.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(8221..8292)
                     /locus_tag="VBC23_001669"
     tRNA            complement(8221..8292)
                     /locus_tag="VBC23_001669"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8258..8260),aa:Gln,seq:ttg)
     gene            complement(8298..8378)
                     /locus_tag="VBC23_001670"
     tRNA            complement(8298..8378)
                     /locus_tag="VBC23_001670"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8342..8344),aa:Tyr,seq:gta)
     gene            complement(8439..8669)
                     /locus_tag="VBC23_001671"
     CDS             complement(8439..8669)
                     /locus_tag="VBC23_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6843603.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(8752..9774)
                     /locus_tag="VBC23_001672"
     CDS             complement(8752..9774)
                     /locus_tag="VBC23_001672"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6843604.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
BASE COUNT         3184 a         1880 c         1622 g         3126 t
ORIGIN      
        1 ttcatgcttt cttttggcaa tattaaattt aatataatac ccactatagc acttaatgct
       61 gttcctgata aagttagagc acctagatca aggactgctc caccgaggcc taaaactaac
      121 atggaactgg caataatcag attacgaacc tcagcaaaat tgacacgatt ttcaatcaac
      181 acttttaatc cattacttgc aataacacca taaagtaata tagccattcc accaagtact
      241 gcactaggaa ttgtagagat caaagctgta aatttaccaa agaatgaaaa agcgattgca
      301 ataaaagctg cattacgaat aaccgttaca gaagctatac gcgtcatgcc aataacacct
      361 gtattttcac cataagtagt ttcagctggc cctcctataa gagcagaaaa tgcggttgct
      421 aaaccatctc caattaaaag acgatttaaa cctggttttt ttagaaagtt acgcccacaa
      481 atctgaccta aaactgtatg atcaccgata ttttcagcaa tcgttacaat tgagattgga
      541 agaattgcaa gcatttctgg tccaaagtaa agtttataac tgtgaaataa acctgtctta
      601 aacggtaaat agaattttgg taattcaaac caggcagctt tttcaactgg agataaatct
      661 accaagccaa gaatgattga taaaatataa cctccaataa ttgcaaataa aaatggaata
      721 atcttgataa aacctttccc tttagtattg ataaaagctg caatcaagaa tgtaacaaca
      781 gcaactagca ttttacgcca atcaccttta gcgacaaaac ctgcattagt tacagcagag
      841 tttgccaaac ctaaaccaat aacaataatc ataggtccaa tgattattgg tggcaaaatc
      901 ttatcaaccc atgaatttcc tacgaactta ataaccgtcg ccacaacgac atagattaga
      961 cctacaaaaa gaatacctgt ttgagcagcc gaaatatcgc catgcatttg tttcatggct
     1021 aatgccatag ctgtaatata agcaaatgat gagcctagat aaacagggac tttaaatttt
     1081 gtcgcaactt gataaatgag cgttccgcaa ccagaagcaa ataaagccac tgatacaggc
     1141 atccctaaaa taagcggaac taatatcgta gctccaaaca tggcaaaaac atgttgaaaa
     1201 cttaaaagca aaccttgaaa taattctggt ttttcatcga tatctagtaa taagttagta
     1261 gaatgtactt ccttatctac tagaatttga ttactttcac ctttagcatt taaagtcata
     1321 tctaaactag tcctcttggg catacaaaaa agcccacttt ctatagcaat atagtcatag
     1381 gctttatttt tttcctgatg tttttctcac ctcacgggat gagtttaaaa acctacgctg
     1441 aaagaattat agcataagac attttagaaa acaagagttt tttgaaacta tattgacaaa
     1501 actatgcttt ttcattatga tagttaacat aataaaatat accgaatgac gtcattcagg
     1561 agaagaaagt tagactttcg ccgaaggaat tacactctca ggtgtcttaa gacaggactg
     1621 attgacagac ggacttctgg agagacctat aagtagcaac atctttgtat tgacaccaag
     1681 atgtgctcta ggcgccgaag gggcaagaag agtaaaacaa ctcctccaat ctctcaggca
     1741 aaaggacaga agctaaaagc caatattaat aatgagtagt aagcttatta agtttactac
     1801 tacctttatt tgtgcgcttt ttagctagca tctttcagaa gttatctctt ttagagataa
     1861 cttctttcgt ttcattacag aatccatagg tatgtcatgt atcaaaggag aacatatgct
     1921 aacacttttt actcatatca atagcttcgt ttggggtcca cctttacttg ctttattagt
     1981 cggaacaggt atttacctat catttcgctt aggttttgtt caattgagac aactttctag
     2041 agctttcaaa ttgattttcc gagaagataa tggacaagga gatatttcaa gttatgctgc
     2101 tcttgcaact gctcttgctg caacggtagg gacaggtaat atcgttggtg tggctacggc
     2161 tattaaatct ggaggaccag gagctttgtt ttggatgtgg gtagccgcct tttttggaat
     2221 ggcaacaaaa tacgcagaag gattattagc tatcaaatat cgcacaaaag atactaatgg
     2281 agaaatttct gggggaccaa tgtactacat tgtcaatgga atgggacaaa aatggaaacc
     2341 attagcagtc tttttttctg cagcaggtat cttagtagct cttttaggaa tagggacatt
     2401 cactcaagtc aatgcaattg cctcttctct tgagcatact ttcaaaatat caactagatt
     2461 taccagcctc attcttgctg ttatagtatt atttattata tttggaggta taaaatctat
     2521 ttcaaaagtt tcagaaaaaa tagtgccttt tatggctata agctatattt tagcaactct
     2581 gattattatt gcagttaatt acaataagat tccacatact ttccaactca tctttagcgg
     2641 tgctttttct gggacagcag ccattggtgg tttttctgga gctatcgtta aagaagcaat
     2701 tcaaaaggga attgctagag gagttttctc aaatgaatct ggattgggtt ctgctccaat
     2761 agctgcagca gcagctaaaa caaaagaacc tgttgaacaa gggcttattt caatgacagg
     2821 aaccttcatt gacactattg ttatttgtac tttgacaggg attgcaattt tagttacagg
     2881 aaaatggcta gaatttgatt tacagggtgc tccgttaaca caagcatctt ttaataccgt
     2941 atttggctca ctaggtagtt ttgccctaac cttctgcctt gtcttatttg cttttactac
     3001 tattttagga tggagctact acggagagcg ttgctttgaa tatctattgg ggactaaatt
     3061 tattaacgct tatcgcatta tttttgtcat tatggtcggc ttaggtggtt tcttacagct
     3121 cgatctcatt tgggttatcg ctgatatcgt taatggttta atggctttac caaatctaat
     3181 agctctccta gctttgtcac caattattgt taaagaaaca cagaaatatt tttcagagac
     3241 taaaaatatc actaagcctt gaacttacta gttttatagt ctgctcctaa gtgatataat
     3301 gtgtagatga aaggaaatta ggaaaatggc aacttctact gaaaacttac aacttgcaaa
     3361 aagagggcct attattagta tcattgccta tataacactg gcagtcgcta aattagctgc
     3421 aggttattgg tttgatgcta cttcacttgt agcagatgga tttaataact tatctgatat
     3481 cttaggaaat gtagcactct taattggttt acacttggct agtcaaccag ccgactcaaa
     3541 ccatcgtttt ggtcactgga aaattgaaga tttagcaagc ctgattacct cttttatcat
     3601 gtttgtcgta ggtattcaag tttttattca aactgtcact aagattatta ataatactga
     3661 tactaacatt gatcctctag gtgctatagt aggtgctata tctgctcttg ttatgcttgg
     3721 cgtttgtttc tataataaac aattatctca aagagtaaag tctagcgctc tggttgcagc
     3781 ttctaaagat aacctttctg atgctgtgac atctatcggc acatctattg ctatcattgc
     3841 ggcttctcta aattttccta ttattgatcg tttagctgct atcattatta cttattttat
     3901 tcttaaaaca gcttatgata tttttataga atctgctttc agcttatcgg atggtttcga
     3961 tgattatcaa ttaaaacaat acgaaaaagc tatattaaca attcctaaaa tatccgctgt
     4021 aaaatctcaa cgtggacgta cttacggtag taatatttac cttgatattg ttctcgaaat
     4081 gaaccctgat ctctcagtct ttgaaagtca tgccattacc gaaaaagtgg aaaaactact
     4141 cagtgataaa ttttctgtat atgacataga tattcatgta gaacccgctt ctatcccaga
     4201 agacgaaatt ttcgataatg tttaccaaaa attatacaaa aacgaaaaaa tcatcctagc
     4261 aaaaatcccc ggttatgaga cttttattag ccctgacttt tatatgatta atgaaaaggg
     4321 aaatattatc acttctgaca tgcttactaa tgctacaaat cattcattgg caagcaactt
     4381 taaatatttc aatgtaaaat caattagtca aaaaacaaaa cttgtgtctt atgaattgga
     4441 aggtaaacga catactagta tttggcgccg taatgaaaaa tggttcttaa tctaccatca
     4501 aataactgcc aagtcaagtc cgtataaaac gagacgatat caaatcactt cccttgatta
     4561 atatactatt taatcagtta ctcttgataa aaatagctta caaaaacaaa ttaaaaagat
     4621 tgagaagaat ctccgtcttt ttaatttgtt tttatgtaaa cgtttcatag ccttgaaaat
     4681 tactttcaga gtagtagact ataatggaag gagtttttca tgaaattata cgttcaatta
     4741 atggttattt taaccttttc attcgctggt gaggttctat ctactatatt caacttacct
     4801 ataccaggta gtattattgg attaatatta ctattcctag cgttaaaata caaaatcatc
     4861 aggctcaggc atattgatac tgtggggaat tttctattag caaatatgac cattttattt
     4921 ttaccaccag cagttggact gatggagcac tttcaagaca taaagcccta tctttttggg
     4981 atagctatca ttatcttagg agcgctattt ctcaatattc taactattgg actggtatcc
     5041 caatggatca aaaagagata tgaaggagat tatccagaaa ttggaggtaa aaatggcaac
     5101 tttaacgaat aaccctatct ttggtattat gttaaccgta tgggcgtatt acattggcat
     5161 aaggattttt agaaaatatc cttcccctgc aacaactcca cttcttttgg cgactatttt
     5221 attaattgct tttttaaaat taacacatat ctcctataaa gattactaca atggtggttc
     5281 atttctaacg atgttaatta cgccttcaac ggttgtattg gctattcctc tctatagaac
     5341 ctttcattta atgaagcatc acattaaaag tattagcatt agcatcatct tagcttctgt
     5401 tatcaacaca gtttttaccg caatagttgc taagtttttt ggtatgaaat atttcttagc
     5461 aatctcactt ttcccaaagt ccgtaactac agctatggcc gttggaatta caagtaaagc
     5521 aggtggatta gctactatca ctctggtcgt agttgttatt acaggcatct taacaagcgt
     5581 tttggggcct atttttctaa aattattaaa aatagaagat cctgttgcta tagggctcgc
     5641 gctaggagga actgggcatg ctataggaac tggacaagcc ttaaaatatg gccaagttca
     5701 aggcgccatg gctggactag caattggtat cacaggaatt tgctatgtta ttgtcagtcc
     5761 tttagttgca ggcttaatat tgaaatgagg aaaagaaaag taaacccaaa ataattatgt
     5821 tataatattt aaaaatagaa tttaggataa ataattatga taccgttaaa aaaaataact
     5881 gttggaacga ttgtctgtct ctcatttttg ggtttaacag catgctcttc ttcaaatact
     5941 cagcaaacct ctacttcaaa atcaaatgta tcacaacata aaaatatcaa agctgaccac
     6001 gaagaattac gtttgaaatt taataaagta aagttagggg ttaaagcaaa taactttaaa
     6061 ggaggcacta gtctcgctga attaaaacaa ttatttggtg gcgaaccaaa tgaaaaattt
     6121 gatacaccag cgggaaatgt cactcttaaa ggctatcgtt ggaatgtcga tgatatctcc
     6181 atcactatac aattattaaa cgatagtagc attgttcgtt caattagtaa ttttaagttt
     6241 attcgtgatg ccaacatcac aacaaaagat tataatagtt taaaaaatgg catgtcatac
     6301 aataaagtaa aagaacttct aggagaacct gatgatattt ctcaagcagt ttcttcagat
     6361 aaagaggaac tacaggctgc atggattagt ggcatccagt cgtcagacag tgacccggga
     6421 attaacctta catttgaaaa tgataaactc actaacaaac aacagcatgg tttaaaataa
     6481 aaaggcatta acgccttttt attaatacct taattactta ttagaaacat tttattacac
     6541 aatggtttaa tcttagtacc ttaacttagc cccttaatgg tcatactaac taattatgat
     6601 actaacttac acagatttca aactgattct cccttatttg tcaaccctag ttttacttat
     6661 cagaaatgag gagaatcagt ctgtgaggca aggttggaga taaactaata taaagcctat
     6721 aacataaagc tttatggagc atctattatc catcttaagg gaatcgaacc cctatcccaa
     6781 gaaccggaat cttgtgtgat atccattaca ctaaagatgg tagtacatgt tacattatag
     6841 catactttat caacaaaaac aaaaagactt gctttagcaa gtcttttaat tattataatt
     6901 caatatcacc gaataagtca gccatagaga atccagtttg tgtttctgga agctcatagt
     6961 cgcgtttttc ttgacgacgt ggacgacgtg gacgtgattg acgtttttct tctttttcac
     7021 cttctgcttg cgctggacgc tcttcaagag ctttcattga aagtgataca cgttctgcat
     7081 ctgaattaac ttcaagaact ttaacagtaa cttcttgtcc tgcagataat acgtctttag
     7141 gattttcaac acgtttgtgt gaaatttgtg aaatgtgtac aagtccatca atgccaggta
     7201 acacttcaac aaaagcacca aagtcagtca agcgttttac tttaccttca ataacatcac
     7261 cagcagcaag tttttgttca acgccatccc atgggccagg tgtagttgct ttaagtgaaa
     7321 gtgatacacg accagcttct tcatcaattg aaagtacttt aacttcaact tcttcaccaa
     7381 cagttacaac tgatttaggt gaaacattgc gctcatgtga taattcagtt acgtgtacaa
     7441 gtccatcaac accaccaagg tcaatgaaag caccaaagct tgttaaacga gcaactttac
     7501 cagttacaac tgaacctact tcaatgtttg agaaaacttc tttacgtgca gcagctgctg
     7561 attcttcaac aacttcacga cgtgaaagga tgaagcgatt ttctgctgca tcaacttctt
     7621 tgattttagc atcaaattct tgtccaacaa atttttcagt gttacgtaca aaacgagtat
     7681 caatcattga agctgggatg aaaccacgaa gtccttcaaa ttcaactgaa agtccgcctt
     7741 taacagcacg tgtaccttta acagtaacaa cttcaccttc acgaccaaca agtttgtccc
     7801 atgctttacg agcttccaag cgttttttag atacaaggaa agttactgta tcagtatctt
     7861 tacctacaac ttgacgaaga acaagtactt caactgtgtc gccagcttta acaaaatcat
     7921 tgatatcagc atcacgatcg ttagtcaatt cacgaagtgt caagacacct tcaacaccag
     7981 taccatcgat aacaacgttt gcttgaccgt tatcaactgt taaaacttcc gcagtgacaa
     8041 cgtcaccagg attcacttcg ctaacactgt ttagcaaatc ttcaaattca ttcattctaa
     8101 aaaatcctcc aacaaaagct agccgagcta gcttttgata acaatatatt ttctcaaggt
     8161 aacccgcaag gacaacaaat atctctttga cggtgtcaaa caaaagtttg atacctttga
     8221 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
     8281 ggctataccc caaataatgg aaagagaggg attcgaaccc ccgaacccga aggagcggat
     8341 ttacagtccg ccacgtttag cctcttcgct atctttccgt aacaacactg actattctac
     8401 cacagatgtt gttagcacgc aagcttttta aataaatatt agcgattaag attatattct
     8461 tcaataatat ttcccatagc ttgatctaaa tttttataga atccactcac ttgctcacct
     8521 ttaatcacag caaatttaga atctagctct gcaatttgac ctacaatttt ttttccaata
     8581 acgagttggt atccatcaac cttagttcca ttaactgtta cttgagcgtc taaaatttga
     8641 atttcaattt tcttatcttt tttactcact attaactcca atatctcttt tattgttttt
     8701 tattgtacac aaaatactaa aagcttgcaa gagcaagctt ttagtataga cttaatctac
     8761 tttataaatc cacccttctg gtgcttcaac atcaccaaac tggatcccta ctaactcatc
     8821 atatagttta cgtgtcgctg gaccaacttc tgtctcactg tagaagacat ggaagtcatc
     8881 tccgttttga attcctccaa ttggtgaaat aactgcggct gtaccacatg cgccagcttc
     8941 tgtgaattta tctaattcgt cgacaaagac atctccttca atcgcttcca taccaaacct
     9001 ttctttagca aggtaaagca atgaatattt agtaattgaa ggtaaaattg aaggactcaa
     9061 aggggtgata aactgattat ctttagtaat tccaaagaaa ttagctgctc caacctcttc
     9121 aattttagta tgtgtcgctg gatcaagata gataacatct gaaaattgtt tctcatgagc
     9181 gtatttccca ggaagaagac tagcagcata gttaccacca acttttgcag ctcctgtacc
     9241 attcggtgct gctctatcat attctttgga aacaataaag ttagttggtg ttaaaccacc
     9301 tttaaaataa ctacctactg gcattgcaaa taccgtaaaa atatattctt cagcaggttt
     9361 aactccaata atatctccta caccaattaa taagggacga atgtataatg ttgcccctgt
     9421 accatacggt ggaacaaact cctcattagc gcgtacaaca gatttaaccg ctgcaataaa
     9481 tttgtctgtc ggaacatgtg gcattaaaag acgatctgct gttctttgaa gtcgttctgc
     9541 attttgatct ggacgaaata actgaataga gccatcttta gttctataag ctttcaatcc
     9601 ttcaaaagct tgttgcccat agtggagggc aggagaactt tctgaaatat gtagcgttgc
     9661 atcatctgtc aacttgccat catcccattt cccatcttta aaatgcgaaa tgtatcggaa
     9721 tggtaatttt ctgtaagcaa aacctaaatt atcccaatct aaatttactg tcataaggta
     9781 cctctttctt tgggtttcta actattgtac gc
//
LOCUS       DATRME010000071         9331 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRME010000071 DATRME010000000
VERSION     DATRME010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..217
                     /gene="essA"
                     /locus_tag="VBC23_001673"
     CDS             <1..217
                     /gene="essA"
                     /locus_tag="VBC23_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO6843605.1"
                     /translation="SNKGQSDRKKRIKQLFSEESNQFKFVTRTEEATVSADIGHMIMY
                     LVLISLVLILASIMTFKIKDKKDEYTY"
     gene            201..443
                     /locus_tag="VBC23_001674"
     CDS             201..443
                     /locus_tag="VBC23_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO6843606.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            443..1717
                     /gene="essB"
                     /locus_tag="VBC23_001675"
     CDS             443..1717
                     /gene="essB"
                     /locus_tag="VBC23_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6843607.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            1724..6155
                     /gene="essC"
                     /locus_tag="VBC23_001676"
                     /pseudo
     CDS             1724..6155
                     /gene="essC"
                     /locus_tag="VBC23_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            6160..6876
                     /locus_tag="VBC23_001677"
     CDS             6160..6876
                     /locus_tag="VBC23_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843608.1"
                     /translation="MKKQRLLLLFGGLLIMIMMTACKDSKIPENRTKKEYQAEQNFKS
                     YFKYISDKNNYLDNIKVYYFSISISKDVQDKVSETTTCSYRLEKQKNQEFIGNFEHEV
                     SESSQYSTEVKNQIQYPIQYKDNSIRFTEKTPSERYDEFVFSSFDSSLLKKYKIYDYL
                     LKHPETELKGVSYKIPINSEIVAPFIKQLNIKNPKKSSISVTKTESKEYYYTISIDTD
                     SEIYSIFEGITLEDVKEFSK"
     gene            6873..7403
                     /locus_tag="VBC23_001678"
     CDS             6873..7403
                     /locus_tag="VBC23_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843609.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            7495..7718
                     /locus_tag="VBC23_001679"
                     /pseudo
     CDS             7495..7718
                     /locus_tag="VBC23_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8050..8526
                     /locus_tag="VBC23_001680"
     CDS             8050..8526
                     /locus_tag="VBC23_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843610.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            8782..9119
                     /locus_tag="VBC23_001681"
                     /pseudo
     CDS             8782..9119
                     /locus_tag="VBC23_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3287 a         1512 c         1690 g         2842 t
ORIGIN      
        1 ttcaaataaa ggacaatctg ataggaaaaa acgtatcaaa caattatttt ctgaagaaag
       61 taatcaattt aaatttgtta caagaacaga agaagcgact gtttcggctg atattggtca
      121 tatgattatg tacctggttc tcattagttt ggtactaata ttagcttcta taatgacttt
      181 taaaataaag gacaaaaagg atgaatacac atattaacat tactctagat atgggggaaa
      241 cctcaaaaga tttacgtatc cctttgaaaa ttagcgttaa gcaattagta atggaattag
      301 atagtatttt tagccgtact gaaaggcgta ctaaatatca actaagagtt ataaataaag
      361 gtattttatt gggagagaac gatatattag cgcaccatcc agtaacaact ggtgatcgta
      421 taaggataga ggagttttat taatggaaac agattcgttt gtatttgaag aacaagactt
      481 tcaatttgaa aaaggaccag atttttggca gttatctctt aagcgttcaa aagtaggaac
      541 tcaaaatctg caacaactgc aattgttaga aattcaccat tctttgttga tgcctatgac
      601 aactgcaatt aatgcagata ccattcaatt tcaattccag acagaagcgc acgctctatc
      661 ttttgaaagc tttaaaaaag aaactttatc tgaaaaatta cgattagcct tgaatgtctt
      721 ggatttagat aaggctttgt ctttgccagt aaatttcatt ttgcatccct ctaatctatt
      781 tttaacaaaa aatgcaactg ctaaaatagc ttatcgtagt cttcctggga ttatgacacc
      841 tgagaagttt ggtccagagg agtttttata tcaattcaaa tgttttgtct ttgcattact
      901 gacgcaacat gactatatag aattgtataa tggtgctatt tctgttattg aagtatcaga
      961 tttcctaaaa agcatttatc atgcagaaac tattcaggct gttagagaca tcattactac
     1021 tgattatgag cagcaagtag aagtagaaac tcatacgtta gcaaaagtat caaaggcaaa
     1081 atataagctt tataaataca taagtgtctg gctaggagct ttatctacaa tactcttgat
     1141 tccgctagta tatctggttt ttattcacaa tccattcaaa gaaaaaatgt tggcagcgga
     1201 tacttcattt attaaagttg attataatca agtgattaat cgactagaac atgtaaaagt
     1261 aagtaagtta ccttatacac agaagtacga gttggcctat tcctatatta atggaatgtc
     1321 attttctgaa gaacagcgtg aagttatttt aaacaatgtt acgctcaaga ctgatgagct
     1381 ctatcttgat tattggatta atattggccg tggtttagat gatgatgcta ttgatgctgc
     1441 caaacgttta gacgattctg accttgttat ttatgctatt gttcagaaaa tggatcaggt
     1501 tagaaaagac aatagtttat ctggtaaaga tcgtgaacaa aaactttctg agctacaaac
     1561 agactatgat aaatattgga aagaccgtaa gacagcctta acagatgagg aatccaaatc
     1621 gaaaaatagc aataatcatt cgacaaattc caacaaagag tcatctgaat catcaagtac
     1681 tacagcaagt acatcttcta aaactaaaag taggtaggtg gatatgttag ttatttgtta
     1741 ttatcaatca ctccgctacg aatttaatct tgaagaggag aaatcctttc taatatcatc
     1801 aaacggcaag tcgccaatac cagtatcaga tttagaaaat gacatcacgc taaaaaatat
     1861 gcaaggtcaa ttggtatata ttattgacca aaaagaaaaa gagttaacta atggtgtaga
     1921 gatttcagga attgtcttct atttagcgaa taatcaaaaa gaaatctata ctcccttaga
     1981 ttacgaagat atattaatcg gtgataaaga agggtatcat gtccgtttta aagaaggagc
     2041 ccctaattta ttactgaaaa aaatagaaag caattggcag cttaatttat ttgaaggtga
     2101 tatttatctg aataatcatc ttcaaaaagt agtgcagcag ttacctttaa gtttaggtga
     2161 tgagattagc ttccaaggaa caattgttaa gcttttccca gatgaaattc aaatctgggg
     2221 ggggggggac tgattatgaa acatcattaa ctaagaaagt tatgtcagct tatcaatttt
     2281 acgaaggcta tcctgatttt caccgctcac ctcgtattat ttatcgttcc agtgaagata
     2341 aaattacagt taatgcgcct ggtaataaac ctaataagtc taaagatgaa ctgttaaaat
     2401 taattgttcc tcctctagtg atgattggag tcagtattct tatttctatt tttcgtcctc
     2461 gtggtatcta tatcattgca acaatgtcaa tggctctagt gacgatgatt ttctctatca
     2521 ctggttactt taaaaatcgt aaacagtata agcaggactt acaagaacgc attgattcct
     2581 atcatgacta tttatctgat aagtcaattg aacttcagaa gttagctaaa gagcaaaagc
     2641 gtgggcaaca ctatcattac ccaacgattg aaggtttaca ggaaatggct gatacttatc
     2701 accatcgtat ttatgaaaag acaccacttc attttgactt cttgtattat cgcctaggtt
     2761 tgggagaagt accaacgagc tataatattc attattctca accagaacgt tctgggaaaa
     2821 aagatccttt agagaacgaa ggctataacc tctactttaa caatcgttat attaaaaata
     2881 tgccgattgt tgcgaattta tcacatggac cagttggcta tataggacca cgaggtttag
     2941 tattggagca actccaattg atggttaatc aattagcttt tttccattct taccatgatg
     3001 ttcaatttat tactattgtt ccagaggaag agatggataa gtggtcttgg atgcgttggt
     3061 tgccacatgc aaccttacaa gatgtcaatg ttcgtggctt tgtttataat caaagaagcc
     3121 gtgatcaagt gctcaacagt cttaaccaaa ttttaaaatt acgtcgcaca caacgcgaag
     3181 ataaatctgc caaagaaggg actttatttt ctccgcatta tgtcgttatt gtaactgatg
     3241 aaaagttaat tttagatcac gtcatcatgg aattttttac ggaagatcct acagaattgg
     3301 gatgttctct catctttgtt caagatgtta tgagttcact ttcagaaaat atcaaaacca
     3361 ttattaatat taaagaccgt aatactggtc agttagttat agaagaagga gagcttaaag
     3421 aaacagattt tgaattagat catttcttag aggattatga caaggaaaat atttcacgtc
     3481 gcctagctcc gttaaatcac ctacaaaatc ttaaatcttc aataccagaa gcagttactt
     3541 ttatggaaat gtatcaagca gaagaatttg aagatttgca tgttcaagag cgttggataa
     3601 gccatgctcc gtataaaagc ttagctgttc cacttggctt acgtggtcag gatgatattg
     3661 tttatctaaa cctacatgaa aaagctcatg gtcctcatgg attagttgca ggaacaacgg
     3721 gatcagggaa gtcagaaatt atccagtcct atatcctttc tttagctgtt aatttccatc
     3781 cacacgatgt agctttcctt ctcatagact acaaaggtgg gggaatggca aatctcttta
     3841 aggacctgcc acacttactt ggcaccatca ccaacttaga tggtgcccaa tcgatgcgtg
     3901 ctttggtttc tattaatgct gaattgaaac gtcgtcaacg tttatttgct aaagctgatg
     3961 ttaatcatat taaccagtat cagaagaaat acaaacttgg agaagtttca gaaccaatgc
     4021 ctcatctctt cttaatctcc gatgagtttg ccgaattgaa atccaaccaa cctgagttta
     4081 tgaaagagct tgtctcaaca gcgcgaattg gtcgttccct agggattcac ttaatcctag
     4141 cgacccaaaa accatctggc gtggtagatg accaaatctg gtcaaactca cgcttcaaac
     4201 tagctcttaa agtagcagac cgcggcgact ccatggaaat gctccatacc ccagatgccg
     4261 ccgagattac acaagcagga cgtgcctacc tccaagttgg taataatgaa gtctatgaaa
     4321 tcttccaatc agcatggtca ggagcagatt accaacctga aaaagacgac caagggattg
     4381 aagaccatac catttattcc atcaacgacc ttggccaata tgaaatccta aatgatgatc
     4441 tttcaggctt agaccaagcc gaaaatatta aagaagtacc tacggaatta gatgccatcg
     4501 ttgaaaacat tcaaacttta accaaagaaa tgggcatctc agacttacct caaccgtggc
     4561 taccgcctct aagcaatcag attgcagtaa ccgatctccg taaggaagaa tcagtagatc
     4621 tctggagtaa agccccatct tacaaagttg ttctgggctt tatggatata ccaagccaac
     4681 aagcacaaga agtagcttac cacgattttg aagatgatgg gcatttgtca atttttgcag
     4741 gtccaagtat ggggaaatcc acagcccttc aaactgtaac attggactta gcacgtcaca
     4801 atagtccaga atttttaaac ctttatctct ttgattttgg taccaatggc ctcttaccat
     4861 taagacgttt gccacatgta gcagatttct ttaccattga tgatgacgaa aaaatagcca
     4921 agtttatcag cagaatcaaa acggaaatgt ctgaccgtaa aaaagccctc agtcgctaca
     4981 atgttgcaac agcaaaactt taccgccagg tatctgggga aaccatgcct caaatcctga
     5041 ttgttattga cagttatgaa gggctacggg aagctcaaac actgactaac ttagaagctt
     5101 gtttccaaaa tatttcgcgt gatgggtcgt cacttggcat ttccttagtt atctcagctg
     5161 gtcgtatggc agctttgagg tcgtccttaa tggcaaacct taaagaacgt atcgctctta
     5221 aattaacaga tgactctgaa tcacggacac ttgtcggtcg tcaccaacac atcatggaag
     5281 atattccagg acgcggatta atcaaacgtg atgacattga agtgttacaa gtggctctcc
     5341 caacagaagg cactgaaacc tttgacatca tcaataacat ccaaaacgaa tctgacgcca
     5401 tgaacagcaa atggactgga ccaagaccaa aagcaatacc aattgttcca gaaacactta
     5461 cctttgagga gtttaaggca tatgaggatg ttaaaaaagc aagtgagact aatcagattt
     5521 cacttggtat tagcttaaaa gaagttgata attgtagcat tccactttcg aaattcaaac
     5581 agttactttt tacttctgac aatccagata atttgtctaa cttgacgcgc ttcttagtta
     5641 aatctgtttc attgattaaa gatagtaagg tttatctttt agacactgct tctgattatg
     5701 atgatttgac agaacaacaa attgaatata tcaataatcg aagtgaaata gcaggacatt
     5761 tagaagctat tgtttcagaa atctatttaa gagaagaatc tggtaaaata gaaccatggt
     5821 ttgttattat tcctgatatg aatagttttg tggcagaaac aaatatggat aaagaacaga
     5881 ttttattctt acttgactcg gctcacaaat atcatattta tttcttattt ggtggcttag
     5941 catcatacct tatgactaat atctcagatg ttccaaaagc tattagaaca caggcacagt
     6001 atgttctctt gggaatgaga gttatggatc agtcggtatt accgaaaaca tataattcaa
     6061 aagaacctta tttgaaacca gatatgattt atattcatga tagaagacaa gagacaatgc
     6121 ttaaaatcac tcaagaaata gaaatggagc attgagcata tgaaaaaaca aagactatta
     6181 ctgctttttg gaggcttatt aataatgata atgatgacag catgtaagga ttcaaaaatc
     6241 ccagaaaacc gcacgaaaaa ggaataccag gcagaacaga attttaagtc atactttaaa
     6301 tatatatcag ataaaaataa ctatttagat aatataaaag tttattactt ttctataagt
     6361 atttctaaag atgtacaaga taaagtcagt gaaacaacaa cttgttcata tagactagaa
     6421 aagcaaaaga atcaagagtt cattggtaat tttgaacatg aagttagtga atctagtcaa
     6481 tattcaaccg aagttaaaaa tcaaatacag tatccaatcc agtataaaga taattcaatt
     6541 cgttttactg aaaaaacacc gtcagaacgt tatgatgagt ttgtttttag ttcatttgat
     6601 tcttcattat taaaaaaata taaaatatat gattacttac taaaacatcc cgaaactgaa
     6661 ttaaaaggtg tttcctataa gattcctata aattctgaaa ttgtagcccc ttttataaag
     6721 caattaaata taaaaaatcc taaaaaatca tctatttcgg ttacaaaaac ggaaagtaaa
     6781 gaatattatt atacaatcag tattgatact gattctgaga tatattctat attcgaaggt
     6841 attacattag aggatgtaaa ggagttttct aaatgagtcc aagtgagaaa ttagatagaa
     6901 gaattgattc ttcatcagac ctctcccagt atgtatatga ttttgaaaag caatatacag
     6961 aatatatgaa gaaggggtat tctgaaaaaa atgcgagtat acaagcacga cgaaccctga
     7021 atgaaatact acaagaaaat aatccaggat tgagatctag cgacgccatg tatgataaag
     7081 aaacaggtat tgcagctatt gctgtaaaag ataaaaatac tggtgagact tatatagctt
     7141 atgcgggtac taataaggat gctgatgggg caaccgactt tatttcagat gcaaatatcg
     7201 gtgctaataa ctctgcttat ttaaaacgat taggagacaa agctactagc ttttatgatc
     7261 gtgttcaaag taatggcgat aatattactg tcacaacagg tcattcttat ggtgactttc
     7321 tcgcttctag agtggcaatt gagctaattc cattctttat taactttaga aaagttaatg
     7381 attcatttca cttttgttat tagcttgtga tcacttataa agtgacagct agttcatgaa
     7441 atttcttagg agttttctgg ctttaaaggt aacctatgat ttaaaaaaca tctcatggaa
     7501 ttcaatgcgg aatcctcaac agtcttttgt ggactaacag aagttcagga tgtcgtttcg
     7561 gcaggcctta actattctca gaaggttttg gaaatttcaa tggtacattt gatacctcag
     7621 gtaagggatt agaatggaca aaaacaattc aaacaggtat gaaaaaacga gaagagaaaa
     7681 tcaaataccc tcattttaca attgttgaca taaagtaatt aaacaaaatc cggaaaattt
     7741 atattaagta atttttctaa aaagtcatat aggcatactt ttaaagttcc tctaaactta
     7801 ataaggaaga aacgttttga aaaaagataa atatattgca ttgaattggt tgtgtgcgat
     7861 agccacgcta tactattaag ttattacaaa ctatgaatta cacctcaaat gttggagata
     7921 aaacctatcg tttggggtgt tttttggtaa aatagaatga aaagagttac tctgtgtcat
     7981 tactaagtta aaaaattata ctaacttaat tattaacgtt ttattgactg agttactctt
     8041 ttttaaaaaa tgtatatcat tccagctggt tactcacctg aacgtttaga ctcagaagtc
     8101 cttacagcaa agattgaaga tgatgaagcc ttattgcgga aaattcaaga ccttaaagat
     8161 catttagata aaaataccaa aatcttggct tacgcttttg atggcttgga agtcagagtg
     8221 aaagctagac taagaaaaaa tcgtaagaaa ctcaaaaacc ttatagaatt caatgccaaa
     8281 tctacctcag tctttaatga attgaaggat tattcaaatg ctgtatcgtc tggtttaacc
     8341 tttgtatcta aaggctttag tagtttcaat ggccagttct cttcagcctc aaatcttgat
     8401 tggcaaaaga ctattaagac aggaatgaga aaacgagaag aagatgctaa acagttgaga
     8461 aaaaaagctt tagaagattc aaataagctc agaaaaattg catcaaaaag gggttacttt
     8521 gaataatgca gttgaatttg ctaaagaaca gaacaaaccc ctaaaaataa aaagataacc
     8581 atagataaat atggtcgtgt aaaaatggat aaaacttttc tttatcgaaa agctaaagat
     8641 ggaaaatctg catatattta ttctgaaggg agtgatcaat ataaagaact aactgggaaa
     8701 gaatttgatc ctaaaactac aaatattatg aaaggtagaa aattagggac agatggtaag
     8761 atatcaattg attttggtga gattagtaac tcaggaaaaa atgcagctat taagtccctt
     8821 aaaaatgaaa gtccttgaat taaatcccaa aaatgctttt aaaggatgga atgagttaag
     8881 caaaatagct aaaactggga aagtactagg atggacaggt aaaattgcta atatttacat
     8941 gaatggaaaa aaagatcttt ttgatgataa aacatcttca atagatcaaa aaatcaaaaa
     9001 ctttgctata ggttcaggtg tagatgcatt gtccggtgca gcaataggga ctttggtagg
     9061 aggacctgtg ggaaccgttg ttggagcagg tgcagggctg gcatttgatt ttgctttgaa
     9121 ttttaaagtt aaggagttgg gtaatgaatc aattacaggt tttgctaaaa aaggtttaaa
     9181 aaatcttgca ggaggattag gattttgaaa aaaagtataa atgtaagtag gtatcatgga
     9241 cctttaattg aatttgaggt tgatgcaagt tatttaagaa ttttttcata tatcggttta
     9301 cctttatcat tgttttggta ttttgttact c
//
LOCUS       DATRME010000072         9252 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRME010000072 DATRME010000000
VERSION     DATRME010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..782)
                     /locus_tag="VBC23_001682"
     CDS             complement(<1..782)
                     /locus_tag="VBC23_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6843611.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /locus_tag="VBC23_001683"
     CDS             complement(782..2125)
                     /locus_tag="VBC23_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6843612.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /locus_tag="VBC23_001684"
     CDS             2265..3116
                     /locus_tag="VBC23_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6843613.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="VBC23_001685"
     CDS             3119..4078
                     /locus_tag="VBC23_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6843614.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /locus_tag="VBC23_001686"
     CDS             4132..5484
                     /locus_tag="VBC23_001686"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6843615.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTVYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="VBC23_001687"
     CDS             5607..5978
                     /locus_tag="VBC23_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6843616.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="VBC23_001688"
     CDS             6003..6383
                     /locus_tag="VBC23_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843617.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /locus_tag="VBC23_001689"
     CDS             6476..7606
                     /locus_tag="VBC23_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6843618.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..8347
                     /locus_tag="VBC23_001690"
     CDS             7610..8347
                     /locus_tag="VBC23_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6843619.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8348..9118
                     /locus_tag="VBC23_001691"
     CDS             8348..9118
                     /locus_tag="VBC23_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6843620.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9108..>9252
                     /locus_tag="VBC23_001692"
     CDS             9108..>9252
                     /locus_tag="VBC23_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6843621.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAE"
BASE COUNT         3054 a         1433 c         1834 g         2931 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcacaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cggtttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt
     7981 tcttgatgat attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc
     8041 gaaatatcat cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt
     8101 agatatgaat ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga
     8161 cttagatttt ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat
     8221 tcaatatgga acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga
     8281 tattattggt ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga
     8341 ggattaaatg gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa
     8401 aagtcgtatc ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta
     8461 tatgttagta actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg
     8521 cttcaatgtt gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta
     8581 tatgaatgat atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa
     8641 agctattgca gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg
     8701 tttagattgg aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg
     8761 tgctttattt attggaacca atccagattt gaatattcct actgaacgtg gtttattacc
     8821 cggtgctggc tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat
     8881 tggaaagcca aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa
     8941 tcaagcagtc atggtcggtg ataattattt gacagatatc atggctggta ttaataatga
     9001 tattgatact ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc
     9061 aattcaacca agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt
     9121 tattagtagt tttaacttgg atttggatta tatcgttagc aactctagct acaatttata
     9181 ttgcttggtt gatctatcct atagaaattc agtttttaaa attagaaaag gttgtgtatt
     9241 taaaggctga aa
//
LOCUS       DATRME010000073         9110 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRME010000073 DATRME010000000
VERSION     DATRME010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            232..1893
                     /locus_tag="VBC23_001693"
     CDS             232..1893
                     /locus_tag="VBC23_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6843622.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            1950..2738
                     /locus_tag="VBC23_001694"
     CDS             1950..2738
                     /locus_tag="VBC23_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO6843623.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            2828..3538
                     /locus_tag="VBC23_001695"
     CDS             2828..3538
                     /locus_tag="VBC23_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6843624.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            3776..4447
                     /gene="rpiA"
                     /locus_tag="VBC23_001696"
     CDS             3776..4447
                     /gene="rpiA"
                     /locus_tag="VBC23_001696"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6843625.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            4504..5715
                     /locus_tag="VBC23_001697"
     CDS             4504..5715
                     /locus_tag="VBC23_001697"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6843626.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            5766..6173
                     /gene="arsC"
                     /locus_tag="VBC23_001698"
     CDS             5766..6173
                     /gene="arsC"
                     /locus_tag="VBC23_001698"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6843627.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            6212..7021
                     /locus_tag="VBC23_001699"
     CDS             6212..7021
                     /locus_tag="VBC23_001699"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6843628.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            7023..8279
                     /locus_tag="VBC23_001700"
     CDS             7023..8279
                     /locus_tag="VBC23_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6843629.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            8263..8973
                     /gene="deoD"
                     /locus_tag="VBC23_001701"
     CDS             8263..8973
                     /gene="deoD"
                     /locus_tag="VBC23_001701"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6843630.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            8982..>9110
                     /locus_tag="VBC23_001702"
     CDS             8982..>9110
                     /locus_tag="VBC23_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6843631.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKE"
BASE COUNT         2887 a         1456 c         1884 g         2883 t
ORIGIN      
        1 tatgatatac taataatgct gtaaatcgac ttgtagagac aaggaccgta agtacaaatg
       61 gaatgaaaat aaggacacac tctttaattc atattcctat tattgattaa taaggttttt
      121 cttcaaaccc aagaaaagtt accgataaat caattactaa tgataataat tggacaattt
      181 cttgtctgct ttataaaaaa gaattaacaa aatttaagga gaaaactcat tatgagcgac
      241 ataaaaatca ttgctcttgg aggtgttcgt gaaaacggaa aaaacctata tgtagtagaa
      301 gtcaatgatt ctatctttgt tttggatgct ggactcaaat atcctgaaaa tgaacaactg
      361 ggtgttgatg ttgttattcc aaatcttgat tacttaattg aaaataaaaa acgtgttcag
      421 gggattttct taacccatgg acatgcggat gctattggtg cattgcctta tattatcgcc
      481 gaagtcaaag cacctgtgtt tggttcacct ctcacaattg aattagctaa gttattcgtt
      541 aaaaatagta cagcagtcaa gaaattcaat aatttccatg tgattgatag tgaaacggaa
      601 attgaatttc aagatgcggt gatttcgttc tttaaaacga ctcattcaat tccagagtca
      661 atgggaattg ttattgggac aaaagaggga aatatcgttt acacaggtga ctttaaattt
      721 gatcaagctg caaggaaata ttaccaaaca gatctggcgc gtttagctga aattggtcgt
      781 gatggtgttt tagcactttt atcagattct gcaaatgcta caagcaatga gcaagttgct
      841 agcgaatatg aggtcggtga tgaaattaag agcgttattg aagacgcaga aggtagagtc
      901 atcgtagcgg cagttgcaag taatctcatt cgtattcaac aagtctttga tgcagcggca
      961 gaaaacggtc gtcgagtagt tttaacgggt tttgatattg aaaatattgt tcgtacggcc
     1021 attcgtatga aacgcattca tattgctgat gaaaatatga ttatcaaacc taaagatatg
     1081 acacgttacg aagataacga attattaatc cttgaaactg gacgtatggg agagccgata
     1141 aatggtctcc aaaaaatggc gatcggacgt catcgttatg ttcaaattaa agatggtgat
     1201 ttagtattta tcgtaacaac accaagtatt gcaaaagagg cagttgtagc gcgtgttgaa
     1261 aatctgattt ataaagctgg tggctcagtt aaattgataa cacagaattt gcgcgtatca
     1321 ggtcatgcaa atggtcgcga attacaatta ttaatgaatc tattgaaacc taaatacctt
     1381 ttcccaattc aaggagagta tagggatttg tcagcacatg cgggcttagc tcaagaggta
     1441 ggaatgtcag cagatgatat ttatattgtg aagcgtggtg acatcatggt tcttgaaaaa
     1501 gatggcttct tccatagtgg atctgttcct gctggagatg ttatgattga tggaaatgct
     1561 attggtgacg ttggtaatat tgtacttcgt gaccgcaagg tattatctga agatggaatt
     1621 tttattgttg ttataactgt cagcaaaaaa gaaaagaaaa ttatttctaa agctcgtgtt
     1681 aatacgcgtg gttttgtgta tgtgaaaaaa agcagagata ttttgcgtga gagcgcagaa
     1741 ttagttaata caacggtaga agattactta agtaaagata catttgactg gggtgaactt
     1801 aaagggaaag tgcgtgatga ggtcagcaag ttcttatttg accaaacgaa acgtcgccca
     1861 gcaatccttc ctgtggttat ggaagttcgt tgatagtgtt aagaggttac caaattttgg
     1921 taacctttct tgctagaatg gaggttttta tggcattttt taatattgaa tatcactcta
     1981 aggtactagg aacggaacgt caagtcaatg ttatttaccc agatgctttt gagatgtctg
     2041 atgataaaat tgatgattgt gacattcctg ttttgtatct tctccatgga atggggggaa
     2101 atgagaattc ttggcaaaaa cgcacaaata tagagcgtct tttacgtcat actaatctta
     2161 tcgtcgtgat gccatcaaca gacttagcat ggtataccaa cacaaagtat gggttggatt
     2221 attttgatgc aatagctatt gagttaccaa aagttttgaa acgtttcttt cctaatatgt
     2281 ctgataagag agagaaaaat ttcattgccg gattatctat ggggggatat ggagcatata
     2341 aaatagctct actgactaat cgtttttctc atgctgcctc tttgtcaggt gcactttctt
     2401 ttgactttga tttattattc aataatggca acgataacac taactattgg tcgggaattt
     2461 ttggtgattt gaataacact gataacatag agcgtcattc actaaggcgt tacgttgagt
     2521 cttttgatat gaagactaaa ttttatgcat ggtgtggtta tgaagatttt ctttttgaag
     2581 ctaatgaagt cgcaattgat gaacttcgtc aattaggttt aactattgac tattccaatg
     2641 atcatggtaa acatgaatgg tattactgga atcaacaatt agaaaaagtc ttagaatggc
     2701 tccctgttga ttatgtcaaa gaagagcgcc tttcttagta ttagctaaat cgttgaaatc
     2761 caagtaaagt cttgttatac taaatcatta agttttggga aattagacac agaaggtgag
     2821 ataaaaaatg agaacactat ttagaatgat atttgctatt ccaaagttta tctttagatt
     2881 gatttggaat atcatttggg gaatattcaa gacagttctt gttattgcga ttattttatt
     2941 tggcttgtat tactatgcga atcacagtca atcagaattt gctaatcaac ttagtgacat
     3001 tattcagaca ggaaaaacat ttttaaattt tgctgacact aatcagctca aaaattcctt
     3061 tgctaatcta gctacggata atgttcatca ttcagaaaat actagatggg ataaaaatca
     3121 ggcaactatc tatatagcaa ctagagattc tgagttggcc tctgcttaca agcaagcaat
     3181 ctccaattgg aatgcaacag gtgcttttgt gttacaaaca acagataatc ctaatgctga
     3241 tattattgct aaggattact cagatgctaa aacacaagct gcaggtgtag ctgaaaccga
     3301 gagaaatgct ttaactaatc gtatttcaaa ggtaacagtt aaattaaaca cattttacct
     3361 taagaataaa cagtttggtt atgaccatac taggattgtt aatacggccg aacatgaatt
     3421 aggacatgct ttaggtctag gacataatga tcaacaacat tccgttatgc agtcaaaagg
     3481 ttcgcattat ggcattcaag aagttgatat tcaaacgcta aaaaacttat atgcataaaa
     3541 agatgagcta ataaactcat cttttttgtt tgaacactta ttttaggtga tataagtgcg
     3601 ttagcaatag attattaaaa ataaagaatt tgactatcac tactaactaa aatgcataaa
     3661 gaacaaagaa aatgttttca aaaatcactt aaaaaatggt ataatgtgtc ttgagatagt
     3721 gttttgaata gacttatcaa aaatattaga taacattaac ttaaaagggg aaaatatgga
     3781 cgaactaaaa aaacttgctg gagtgaccgc tgcaaaatac gttaaaaacg gtatgattgt
     3841 gggtttaggg acaggttcta cagcatattt ttttgttgaa gaaattggac gccgtgtcaa
     3901 agaagaggga ttgcaagtca taggcgttac aacgtcaaac cgtacgactg agcaggcaag
     3961 aggattaggt attccgttga aatcagcaga tgatattgat gtcattgatg tcactgtgga
     4021 tggagcagat gaagtagacc ctgattttaa tggtatcaag gggggcggtg gtgcgctgct
     4081 catggaaaaa atagtagcta ctccaactaa agaatatatc tgggttgtcg atgagagcaa
     4141 acttgttgaa acgttaggtg catttaaatt gcctgttgag gttgttagat atggttctga
     4201 gcgtcttttt cgggttttta aatcaaaagg ttattgtcct tctttccgtg aaacagaagg
     4261 tgaccgcttt atcactgata tggggaacta catcatcgac ctagatttga aaaaaataga
     4321 ggatccaaag cagttagcga atgaacttga ccatacagtt ggtgttgttg aacatggttt
     4381 atttaatggt atggttaata aagttatagt agcaagcaaa aatggtcttg acatacttga
     4441 aaaataatta tttaaataat cggttttagg agctaaccgt aacctcctta gaaagaagat
     4501 ataatgtcac aatttgatcg cattcactta gttgtcttag attcagtggg tattggagcg
     4561 gcaccagatg ccaatgattt tgtcaatgca ggagttcctg atggtgcttc agacacgctt
     4621 ggccacattt caaaaacagt tggacttgcg gtgccaaaca tggctaaaat cggacttggt
     4681 aatattcctc gtccccaagc gcttaagacc gtccctgcag aagaaaatcc aagtggctac
     4741 gcgacgaagt tacaagaagt atcacttggt aaggatacaa tgacagggca ttgggagatc
     4801 atgggcttaa atattacaga gccatttgat actttctgga atggttttcc agaagatatt
     4861 atcacaaaga ttgaggattt ttcaggacgt aaagttattc gtgaagcgaa taaaccttat
     4921 tcagggacag ctgttattga tgacttcgga ccacgccaaa tggaaactgg tgagctcatc
     4981 atctatacct cagctgatcc agtcctacaa attgcagctc atgaggatat catccctcta
     5041 gaagaactct accgtatttg tgaatatgct cgtagtatca caatggagcg tccagctctc
     5101 cttggtcgta ttatcgctcg tccatatgtg ggtgaaccag gcaactttac gcgtacagcc
     5161 aaccgtcacg actatgcagt atcaccattt gaagataccg tattaaataa actagatcaa
     5221 gctggcatcg atacttatgc tgttggtaaa atcaatgata ttttcaatgg ttcaggcatt
     5281 aatcatgata tgggacataa caaatcaaat agtcatggta ttgatacttt gataaaaacg
     5341 atggggttat cagagtttga aaaaggcttc tcctttacta atttggttga ctttgatgct
     5401 ctctatggtc accgtcgtga tcctcatggt tatcgtgatt gtcttcatga gtttgatgag
     5461 cgtcttccag agatcgtttc agcgatgcgt gacaaagact tacttttgat cactgccgat
     5521 catggtaatg acccaactta tgcaggtaca gaccatacaa gagaatacat tcctctctta
     5581 gcctatagcc catctttcac aggaaacggc cttatcccag tagggcattt cgcagatatt
     5641 tcagcaacag ttgcggataa ctttggtgtt gatactgcga tgattggaga gagcttcttg
     5701 caggacttgg tataaatttt tatttcctta agacacttgt agcattaaaa attgagagga
     5761 agatgatgga aaaagttagg atttaccata acccaaattg tgggacatca aggaacgtac
     5821 ttgccattat tcgtcattgt ggtattgaac cagaaattat ttattatctg aaaacaccac
     5881 ctagcaagat ggcactagtt gaactgttgt tagaaatgaa actttctgtg agagaacttc
     5941 taagaacaga tgttcctgct tatgagaaat tcaatttgga atcttctagc gttacagatg
     6001 aagaaatgat tgatgcgatg atacaagatc ctgttcttat caatcgtccc atagttgtta
     6061 cgagtaaagg tgcaaaactc tgccgaccct gtgaagaaat tttaaccatt ttacctgtaa
     6121 agatggaaaa agattttgtt aaagaagatg ggcagattat ccaaagttta tagcatattg
     6181 tatagtgaat aatagtttag gaggtaagta aatgacatta ttagaaaaaa ttaatgagac
     6241 tagagacttt ttgcaagcaa aaggcgtcac agcaccagaa tttggtctta ttttaggctc
     6301 tggtttagga gaattagctg aagaaatcga aaatcccatt gttgtggatt atgcagacat
     6361 cccaaattgg ggacagtcaa cagtagttgg tcatgctgga aaattagtgt atggggattt
     6421 atcaggccgt aaggtattag cgcttcaagg tcgttttcac ttttatgaag gaaatacaat
     6481 ggaagtcgtt actttcccag tacgtatcat gagagcattg gcttgccaca gtgtgcttgt
     6541 gactaatgca gcgggtggga ttggatacgg accaggaact ttaatgctga tcaaagacca
     6601 catcaatatg attgggacta accctctcat aggtgagaac cttgaagaat ttggaccacg
     6661 tttcccagac atgtcggatg cttatacagc aacatatcga caaaaagctc accaaattgc
     6721 tgaaaaacag aatatcaaac tcgaagaagg tgtgtacttg ggtgtatcag gacccactta
     6781 tgaaacacct gcagaaattc gtgcattcca aacaatgggc gcacaagcgg taggtatgtc
     6841 cacggttcca gaggtgatcg ttgcagctca ctcagggctt aaagtgttag gaatttcagc
     6901 aattactaac tttgccgctg gcttccaatc agagctcaat catgaggagg tcgttgaagt
     6961 tactcagcgt attaaagaag atttcaaggg attagttaaa tcattagttg ctgaacttta
     7021 aaatggtttc tcgattatac tatgctgtca agtttatgat agccgttcta tttatgacag
     7081 ttatggcagg agttggagct atcttaatgc actatgtatt aatgtttaca gagtggttag
     7141 catttggtga tagtagagaa aatactctgt ctttgttaaa tagtgtcaca ccaattaaaa
     7201 gagtgttatc acttacttta gtctcctttt tagcatccct tagttggtat tacttacaaa
     7261 tcaagccaaa gcaaataact agtattaaac aacaggttgt ttttaaggat ttttctgtaa
     7321 aaaaatcacc ttactggcta catataggtc acgcattttt gcaattgatt tatgtaggag
     7381 ctggtggtcc tattggaaaa gaaggagcgc cacgagagtt tggagccata aatgctggga
     7441 aaatatcgga tttattagca cttaaagtgc tggataaacg tttattaatc gttagtggtg
     7501 cagctgcagg tttgtcagca gtttatcaag tacccttagc tagtgtattc tttgcttttg
     7561 aaactttagc tttaggaatt tcactgaaaa atattgtgac attgctagtc tctacttttg
     7621 gtgcagctag tattgctcaa ttagttattt cgacagcccc tctttatcat atttcaaaga
     7681 tgagtctgaa ttcacagtca ttagctttta tgttcttgat cgttttatgt gtaactccga
     7741 ttgcgattag ctttagatat ttaaatcaaa aagtgacaga gagacgtatt aaaaatataa
     7801 aaatattgtt aagtttgcca gtagtgtcat tgatagtatc tgttttatca ataatctatc
     7861 cccagatttt aggaaatggt aatgccctag ttcaagaagt ctttaaaggg accacagtta
     7921 gcttaatcgc tattttagtt gttctcaaga tgatagcaac attatccact ctttacgctg
     7981 gtgcttatgg cggtatactg acaccatctt tttccattgg tgcttgttta ggttttttgt
     8041 tagctagtat ttctattcct ttacttcccc acctatcgat agtaaccagt atgttggttg
     8101 gtgcagctat ttttttagct attacaatga gagcgccact gacagctgtc ggtttagtca
     8161 tatcttttac tggtcaatct gttataacta ttgttcccct aactatagca gtactttttg
     8221 ctactgccta tgattatttt ataagaaaaa tgaggagttt atatgtcaat ccatattgaa
     8281 gcaaaacaag gtgaaattgc agataaaatt ttacttccag gagatccact ccgtgcgaaa
     8341 tttattgccg agaatttttt agaagatgcg gtgtgtttta atacagttcg caatatgttt
     8401 ggttatacag gtacctataa aggacatcgt gtttctatta tgggaactgg tatgggtatg
     8461 ccttcaattt caatttatgc tcgcgaacta attgttgatt atggggttaa aacattgatt
     8521 cgcgtaggaa cagcaggtgc tattaatcct gatatccatg ttcgtgaact tgttttggca
     8581 caagcagcag caacaaattc taatattatc cgtaatgatt ggcctgaatt tgattttccg
     8641 caaattgctg attttaaact tttggataaa gcttatcaca tcgcaaagga aatggatatt
     8701 acaactcatg taggcagtgt attatcttca gacgtgtttt attcaaacca accagatcgt
     8761 aatatggctt tgggtaaact aggtgttcat gctattgaga tggaagcagc agctctctac
     8821 tatcttgcag ctcaacacaa tgttaatgct cttgctatga tgacgatttc agacaacttg
     8881 aataatccag aagaagatac aagtgcagaa gagcgtcaaa caacatttac tgatatgatg
     8941 aaagttgggc tcgaaacact tatttctgaa tgaggataga aatgacagat ttttcaactg
     9001 ctcttaaagt attagtggaa cagtattctt atcataatgc tttcttatta ttacgaaaac
     9061 atggtccttt aaactcagat ttattatttt tattagaaat gatgaaagag
//
LOCUS       DATRME010000074         9054 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRME010000074 DATRME010000000
VERSION     DATRME010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(32..1339)
                     /gene="eno"
                     /locus_tag="VBC23_001703"
     CDS             complement(32..1339)
                     /gene="eno"
                     /locus_tag="VBC23_001703"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6843632.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            1537..1992
                     /locus_tag="VBC23_001704"
     CDS             1537..1992
                     /locus_tag="VBC23_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6843633.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            2005..2490
                     /locus_tag="VBC23_001705"
     CDS             2005..2490
                     /locus_tag="VBC23_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6843634.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(2511..3152)
                     /gene="serB"
                     /locus_tag="VBC23_001706"
     CDS             complement(2511..3152)
                     /gene="serB"
                     /locus_tag="VBC23_001706"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6843635.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(3246..4970)
                     /gene="ezrA"
                     /locus_tag="VBC23_001707"
     CDS             complement(3246..4970)
                     /gene="ezrA"
                     /locus_tag="VBC23_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6843636.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            complement(5064..7016)
                     /gene="gyrB"
                     /locus_tag="VBC23_001708"
     CDS             complement(5064..7016)
                     /gene="gyrB"
                     /locus_tag="VBC23_001708"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6843637.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(7017..7577)
                     /locus_tag="VBC23_001709"
     CDS             complement(7017..7577)
                     /locus_tag="VBC23_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6843638.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(7696..8901)
                     /locus_tag="VBC23_001710"
     CDS             complement(7696..8901)
                     /locus_tag="VBC23_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6843639.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
BASE COUNT         2864 a         1828 c         1418 g         2944 t
ORIGIN      
        1 attatatttt caactgttta ataatctgag actatttttt taagttgtag aatgatttga
       61 tacctttgta ttgagcaact tcaccaagtt ggtcttcgat acgaagtaat tggttgtatt
      121 tagcaatacg gtcagtacgt gataatgaac ctgttttaat ttgaccagcg ttagttgcaa
      181 ctgcgatatc agcgattgtt gaatcttcag tttcacctga acggtgtgat acaactgcag
      241 tgtaaccagc ttctttagcc atttcaatag cttcgaaagt ttctgtcaaa gtaccgattt
      301 ggttaacttt gataaggatt gagttagcag cttcttcttt gataccacgt gctaagtaat
      361 cagtatttgt aacgaagaaa tcgtcaccaa ctaattgtac acgtccacca aggcgttctg
      421 taagagcttt ccaaccatcc cagtcgtttt catccatacc atcttcaata gtgatgattg
      481 ggtatttgtt aaccaactct tcaaggtaat cgatttgttc tgcagcagta cgaactgctc
      541 caccttcacc ttcgaattta ctgtagtcgt atactttacg ttctgcatcg tagaactctg
      601 atgaagcaca gtcaaaacca atcatgattc cgttttcgcc agcttcgtaa ccagctgctt
      661 caatagcttt aagaatagtt tctacgccat cttcagtacc ttcaaattta ggtgcaaatc
      721 caccttcatc accaacagct gtttcaagac cacgttcttt aaggattttc ttaagagcat
      781 ggaatacttc agcaccccaa cgaagtgctt ctttgaaagt tggtgcacca actggcataa
      841 tcatgaattc ttggaatgcg attggagcgt ctgagtgaga accaccgttg atgatgttca
      901 tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa agtggtactt
      961 caaggtaatc agctgcagca cgtgctacgg caatagaaac accaagaata gcatttgcac
     1021 ctaatttacc tttattagga gttccgtcaa gagcgatcat agcacggtcg atagcttgtt
     1081 ggtcgcgtac atcataacca atgattgctt ctgcaataac gttatttaca ttgtcaacag
     1141 ctttttgtgt tccaagacca ccgtaacgag acttgtcgcc gtcgcgaagt tcaactgctt
     1201 cgtgttcacc agtagaagct cctgaaggaa ccataccacg tccgaaagca cctgattctg
     1261 tataaacttc tacttcaagt gttgggttac cgcgtgagtc aaggacttcg cgtgcgtaaa
     1321 catcagtaat aattgacatt acaatactct ccttatgagt tttaattttt tacatattca
     1381 taatatcata atctttagtt aataacaagg aaaaacccag tgaaaccgct ttattacgtg
     1441 gtttttgata aaaaaatcac taattctttt caagtttacc tgtgctaatg caacccattt
     1501 aggttataat aaaattagca aattcaagga gaaattatga ctgaacttga caataaaatc
     1561 ctacaaaaag caagcggtga aaatcgtctt gatcctgatc aacaacgtta ctaccttggt
     1621 acttataaag aaagagttgt gctccactta agtttaaaag aggcacaaga agaaaagttt
     1681 caacttgcta taacatctgc tcttaataat ctgatacttg aatatcctaa tcttagttta
     1741 aaattatcac caaatctccc tactgatgtt caaatgttct acatgaagtt agctaaccgt
     1801 ctatcactag ctgctacgat tgttaatgaa gcaaatgcca attctccatt tggtattatt
     1861 atacatagtg ataaggctga aaacgttgaa tggaacgatt ttgagacaca attccctgat
     1921 ttattcaatt caccaaaaaa agaggagtct cctaaaaaaa gtctatggca acatttcttt
     1981 agtcaaaaat aacaaggagg tctaatggaa atttgggatg cttatgacca atatgggttc
     2041 attacaggtc ttacacttaa tagggatcaa aatatcccac aaggcttatt ccacttagtt
     2101 gttgatgtta tcctttttca tgaggatggt gacgtattga tgatgaaacg gcaccctaaa
     2161 aagaaagctt ttcctgctta tttcgaagcc accgctggcg gatctgccct taaaggagaa
     2221 aatgctaagc aagctattct aagggaactc aaagaagaaa ctggtatcgt tcctcaatgt
     2281 ctaacctttc ttaatagaga gtggttctct gagcgttctt actttgtaga ccattttatc
     2341 gcaaaataca atggtgcaaa agatataatt acattgcaag aaggagagac cgttgactat
     2401 atctggttaa aaccagaata tattgattta tttttatcta aaaacaaatt aataccctct
     2461 caaataaaac tcttaaaatc cttaatctaa aaactaggct taataaaggc ctagttttca
     2521 tttgcatcaa taatatcaaa taccttaagc aaattttttt cattaatctg atacgcagct
     2581 tcttctctga caattggttt tgcacaaaaa gcaattccta ccccagcgga tttgatcatt
     2641 ggtaaatcat tggcaccatc tcccattgcg atggtttgtg aaaggtctaa gtgattttca
     2701 gaagcccact cttttaactt tatttttttc acttctttag tgacaatctc tccctcaact
     2761 tcgccagtga gaaaaccacc tttgacacca agacgattcg ctttgacata atcaacttgt
     2821 aattgcttag ctaagatatc aatcgtctcg tgaaaacctc ctgatacaag accaactttc
     2881 atctgacgct tgtgacactc tttaatcaat tcataagccc ctggagtaaa atgtattgag
     2941 gataatatat ctgtcaaaat agtaacaggc aaacctttaa gtagcgctac tcttttcttc
     3001 aaagcttctt caaaatctaa ctcgccacgc attgcagcgt cagtcagtgc agcaacctgt
     3061 ttccccaccc ctgcttcaat agctaataaa tcaatagctt cttccattat taaggttgaa
     3121 tcaacgtcca tgactaacaa acctttaatc atttaaaacc tccaaaactt ttttactatt
     3181 gtaccttaaa attagcacct ttcatagata gcaattaaaa aaagaagagt actaactctt
     3241 cttttttaga aacgaatacg ttcttgtgtc ttttcataag acgtcacaaa acggtcagtt
     3301 acacctggtt ccacagtctc aagtgcgtat gaaatttcat cgaaagatgc tttatagtta
     3361 tgttcaactt caaataagta caaagcttgt tcaaagctct tttgcacatt ttgttcaaaa
     3421 ctacgataac ggtttgaata ctgtagtaac tgctctgtga gagtcgcatc ttgaacaact
     3481 agatatgtag cttgctctaa attagcgata gcatttgtag tcacatcatt taaacgtgaa
     3541 acagcctcaa tatcaatacg accgcgactg agttcattaa taagtgcctc aatttgagaa
     3601 cttgttgtaa agaaagtgct taagaagtct tgcgggattc ctggaagatt gcgtttttcc
     3661 atgaaacgtt taatcatatg gagcttgtta acatacttat ccatattttg gcgtgctcgt
     3721 gtctcaacgc tttcaatagt tttaacggct tcaaaaactt ccatttgatt ttcttcaaca
     3781 gtagtcaatg ttttcaacgt acgctcaaat aaaatttgta gatgagtata aggtgaagct
     3841 tcttgaccaa aattttctac agaaggtaaa acctttgttt caatttcttc caaatcattt
     3901 gtgaaggaac gtaaacttaa gctttcattt tcatttaaga tatatttagc agataatcgt
     3961 ttgatttcat gccccagttg ttcattgtta gattttgcat gagcaaggaa ttgaggaata
     4021 attttactat ccttatgagc tactttataa gcagcaatct cacgttcaaa aatatcataa
     4081 agtttatcaa ttttttcttg aatatgttca ttttcatcgc gagcacgatc tagatccaaa
     4141 ctaactaaac catctgaatt cgaacgaata gcctcacgaa cttcttgaaa acgttgttca
     4201 atatcctttt cagggaaatg gtagttttcc tctaaaagac gacgatatcc tgtctccaaa
     4261 tcatctaact gatctggaaa atcgtcttct aatttagcaa caatagctgg aatttgttct
     4321 gtaatttgcc ctaaagcaat cgtatgctcc tctgctttat tcaaaacttc agaagcttca
     4381 atcgggtcac cagttgagtt taaggtcaca aaatgtgaaa attcagcctc gatatttttc
     4441 aactgctttt caatttctgg catggttgtc ccgtaattat cttctttttc agaaattgat
     4501 ttttgaagtg tttcgtatag gtccaaagca tgtgttacac gggcactatt tttctcttct
     4561 tgctctttta aaacttctaa agcttcacga atcgaaacaa tatcttcttc tataatagtt
     4621 agctgactat caacattatc aatctcgtgc ttagcacgca caaaattaaa tgaatcatta
     4681 agattttcag cttcaactaa atgaaaatca atatctttaa atgaattagt tgataaatca
     4741 gtccattttt ggttccattc tctaaaagtt gattgacttt ggccaatcaa atgaagcaat
     4801 ttgacttgct caatctcctc ttgcacagga aggtcaacta actcttgttt tctagtttct
     4861 aagtttgcaa ttaatgtatc attacgtttt cttattacga cgccgactac gtaggcaata
     4921 atcacaagca ggactatagc cacaagcagc aatatgattc cgctcgacat tcaactctcc
     4981 ttataatggt ctaattttct aaaaatctac tataacgtta agattatagc atacttgaac
     5041 tgtttttcaa agctatcttt ggactagata tctaagttac tataaacagc attagcttca
     5101 atgaattcac gtcgtggttc tactcgatct cccattagca tatcgaaaat cttatctgct
     5161 tctgctgcat catcaactga gacacgtgcc atcaagcggt tttcaggatc cattgttgtt
     5221 tcccaaagtt gatggtcatc catctcacct agacctttgt aacgttgtac agtaggtttt
     5281 gaacgtcctg aactataggt atctagagct tgacgtaatt cctcttcttg attgacccct
     5341 ggctgaatat aagctttaat ctcacttcct actttgacac catagattgg tggttgagcg
     5401 atataaacat atcctgcttc caaaacaggt cgcataaaac gataaatcag agttaaaagt
     5461 aaagttctga tgtgagctcc atcaacatcg gcatcagtca taatgactaa tttttgatat
     5521 ctgacttttg atacatcaaa atctgcacca aatccagttc ccatagctgt gaagagactt
     5581 cggatttctt cgtttgctaa aattttatcc attgtagctt tttcaacgtt tagaatctta
     5641 cccctaatcg gtaaaattgc ttgaaactca cgattacgtc ctgattttgc agaccctccc
     5701 gcagaatctc cctccactat aaaaagttca ttcatctcag cattattaga tgagcaatca
     5761 gccagtttcc caggtaagtt tgaaatctct aagcctgact ttttacgtgt tacttcacgc
     5821 gcccgttttg ctgcaatacg ggctttagat gccaaaattc ctttttcaac aatcttctta
     5881 gctacttgtg gattttctaa taggaatcga ttgaaagctt cgctaaagag acgatttgta
     5941 attttaacaa cttcagaatt tccaagtttt gttttagttt gtccttcaaa ttgtggattt
     6001 ggatgtttaa cagatataac tgctgttaaa ccttcacgaa catcttctcc agttaaatta
     6061 tcttcatttt ctttgagaat cttattcttt ttagcgtaat cattaatcac acgtgtcaag
     6121 gcagtacgaa atccttgttc gtgcgttcct ccttcatgag tgtggatgtt atttgcaaaa
     6181 ctcataacag tctcttgata tccagtagta tactgcattg ctacttcaac tgaaatacca
     6241 tctaattcac cgtcagtata aataggattt tcaaaaatca cttctttgtt ctcattgata
     6301 aactcaacgt atgaacctat accaccttca tagtgatatt ctttttcaac ttcttgacct
     6361 tctcgcttgt ctgaaatgga gattcttagt ccacgattta aaaaagctaa ttcctgaata
     6421 cgtttggcta atttatcaaa atcaaataca gtggtttcag tgaaaatttc tgggtcaggc
     6481 gtaaagtgaa ctgttgttcc tgataagtca gtatcaccga taatttcaag atcattaaca
     6541 acaacaccgc gttggtattc ttgataatga accttgccat ttttataaac cttgacatct
     6601 aattgtgttg acaatgcatt aacaacagat gaccctacac catgtaagcc accagaaacc
     6661 ttgtatccgc caccaccaaa tttaccaccg gcatggagaa ctgtaaaaac tgtctctaca
     6721 gctggtcttc cagttttttc ttggatatct accggaatac cgcgaccatc atcaacaact
     6781 gtaatagagt tatctggttc aatgtaaact ttaatatgtc ctgcaaatcc agctaaggct
     6841 tcatcaattg agttatcaac aatttcccat acaaggtgat gaagcccttc tttcgaagtc
     6901 gaaccgatat acatgcctgg acgcatgcga acagcttcta aaccttctaa tacttgaatc
     6961 tgacttgcgt catattcttg agcacgttgt tccatattct ttgtttcttc tgtcatttac
     7021 tctattatct ccatcaatga ttttgaccca tcgactaata aggtatcaaa accagccgat
     7081 tctcctgctt gcttatctat atcacgatcg cctattacta aacaattatc aatttggtat
     7141 ttttctttca aataaagcat actctcggga ctaggtttcc ttgaaaagcc gttatcagca
     7201 gtcactactt ctgtaaaata gtcaataatt tctgtttttt ccagaataac tataacttga
     7261 ttatcacgat gagacaccaa aaaattacga ctaccttgtc tttgaatttt cgctaaaagt
     7321 tcttttgccc cttggaatag aattggtgtt tccaaaacct tagcttcatt agccttatac
     7381 tcttttaaaa aatcagcttc ttcagggata aaatatgcaa ttgcatctgc tgttgactct
     7441 tttaattttt gatacacact atcgtggtct gcttgatatc caaattcttt caaagtctct
     7501 acaaaagcac gtgtggaact ttcgtaatta tcaagtagtg taccacccaa atcccaaata
     7561 taatcatgat aattcatacc ctttattata tcatattatt acgatttttt atagctacca
     7621 ccttacttaa ttaactcact cctctaaaat atttcgatta ttaaaaggct ggattatctt
     7681 tccaaccttt ttttgttata tactacgata cttcaaatgt gatccattag ccttggcctg
     7741 acgaactgta ttttgataag acatggataa gaccaatcca attccaataa gattacttag
     7801 taatgaactt ccaccttgcg aaataaacgg aagtgggatt cctgttagag gtaatatacc
     7861 aactgctgca ccaatatttt caaaaacatg gaaaacaatc atcatgataa aaccagttga
     7921 tataaaagta taaaattgat tattagattc aatggtgatc cttaacatac ggtaaatgat
     7981 aatgagatat aaccctaaaa caatcgccga accaatgaag ccaaaattct cagcaatgac
     8041 agtaaaaatc atgtcacttt cacgaacagg aaccgagagc tctaaaatat taaaaccttt
     8101 acctgttacc ccgccacttc caattgatac cataccctgt gtttgttgat aagcaattga
     8161 gtctgcaaaa ctaaatggat caatccaagc agataaacgg ttgatttgat atgtatccat
     8221 ccccaaatta taaaaccatt ccttgccgtt tggtgagata aaaatcatca aaaagctagc
     8281 tataaaaaga acgatagtag ataaaattgg caagattatc caccaagata tgcccgataa
     8341 aaggattaaa cctgacaaaa tggcaaggaa aactaatgct gttcctaaat ctttttgtaa
     8401 cattagaaga atcatcactg gcaatgtaac caaaccaaac aatcctagca acttccaatc
     8461 atcttgaaaa gttttgcgat ttttctgatg aaaactaaca gttattcgag ataacattaa
     8521 aatataggaa atcttcataa actctgacgg ttgaaacaaa gttaccgagc caatggtcac
     8581 ccaattttta gcacctgttg ccgcaaaaag ctgtggacta taaaaaatta agggaagaac
     8641 cattagggtt agtcctaaag catacagata gggcgtggct ttccataaaa attcagtact
     8701 gaaaagcata acgacaaatg caataataca ccccataata atccaagaca cctgctgaaa
     8761 catcgcgata tagatattag aaggataatc attcatcgtt gcaacataaa ttgaggctaa
     8821 accaatcatt aataaaaaaa atactggtat cacaacagca taatcaattt gccctttaaa
     8881 atagtttgat ttttgtgaca taggacttcc ttttttattc atggtaatta ttataacatt
     8941 ttttcagaat ctgacttata ttttttgcaa taaaaaatgc aactcaaatc attatgagtt
     9001 gcaaatatta ggagattgtt cttcatcagt aaaacaaaga acatgaaaag aaaa
//
LOCUS       DATRME010000075         8829 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRME010000075 DATRME010000000
VERSION     DATRME010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            84..470
                     /locus_tag="VBC23_001711"
     CDS             84..470
                     /locus_tag="VBC23_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6843640.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            576..2855
                     /gene="pcrA"
                     /locus_tag="VBC23_001712"
     CDS             576..2855
                     /gene="pcrA"
                     /locus_tag="VBC23_001712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6843641.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            3267..3512
                     /locus_tag="VBC23_001713"
     CDS             3267..3512
                     /locus_tag="VBC23_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6843642.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            3560..3808
                     /locus_tag="VBC23_001714"
     CDS             3560..3808
                     /locus_tag="VBC23_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6843643.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            3823..4404
                     /gene="amaP"
                     /locus_tag="VBC23_001715"
     CDS             3823..4404
                     /gene="amaP"
                     /locus_tag="VBC23_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6843644.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            4414..4608
                     /locus_tag="VBC23_001716"
     CDS             4414..4608
                     /locus_tag="VBC23_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6843645.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            4664..5206
                     /locus_tag="VBC23_001717"
     CDS             4664..5206
                     /locus_tag="VBC23_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6843646.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            5253..5450
                     /locus_tag="VBC23_001718"
     CDS             5253..5450
                     /locus_tag="VBC23_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6843647.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            5472..6023
                     /locus_tag="VBC23_001719"
     CDS             5472..6023
                     /locus_tag="VBC23_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6843648.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            6187..6828
                     /locus_tag="VBC23_001720"
     CDS             6187..6828
                     /locus_tag="VBC23_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6843649.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            6833..7597
                     /locus_tag="VBC23_001721"
     CDS             6833..7597
                     /locus_tag="VBC23_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6843650.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            7597..8256
                     /locus_tag="VBC23_001722"
     CDS             7597..8256
                     /locus_tag="VBC23_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6843651.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQETYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            8329..8823
                     /gene="tpx"
                     /locus_tag="VBC23_001723"
     CDS             8329..8823
                     /gene="tpx"
                     /locus_tag="VBC23_001723"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6843652.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
BASE COUNT         2970 a         1250 c         1805 g         2804 t
ORIGIN      
        1 aaactttaaa ataaaaacgt ttatagttat ggtttcctta ttttctgcta taatggtcat
       61 agaaaaagtg aggttatagt agaatgaaat tcaatttaga acaagtaaaa gtatttgaga
      121 attacgaaat tgaaaactgg gaagaaggtc aagttacctt aacaacgaag gtcgttgact
      181 cttcgctcaa ctattatggt aatgctcatg gtggttattt gtttacactg tgtgatcaag
      241 tcagtggttt agttgctatt tcaacaggat atgaagcggt aactctacaa tcaaatatca
      301 actatttgag ggcaggccga ttagatgatc tgcttactgt catcggtact tgtgttcata
      361 atggtcgtac tacaaaagtc gttgatgttc acattaaaaa tcagaaagac gaaatggtta
      421 caactgctat ttttacaatg tttgtaactg gagagagaaa agagaagtaa aaaaggtagg
      481 agttagacag cgtctgttta actccttttt ctattgggaa aaagatgaaa aaatgatata
      541 ctagagaggc aattttgaca aaattaggag tagaaatgaa tcctttaata attggaatga
      601 atgataaaca agcagaagcg gtacaaacga cagacggacc gcttttgatt atggcaggag
      661 ctggctctgg aaaaacacgt gttctgactc atcgtattgc ttatttaata gatgaaaaat
      721 atgttaatcc ttggaatatt ttagcgatta cttttactaa taaagcagcg cgtgaaatgc
      781 gtgaacgtgc tatcgccttg aatccagcta cccaagatac tttgattgca acttttcata
      841 gtatgtgtgt tcgtatttta cgtcgtgaag ctgattatat tggatataat cgtaacttta
      901 caatcgttga tccaggtgag cagcgtactt taatgaagcg aatcattaag caattaaatt
      961 tggacacaaa aaagtggaat gaacgttcaa ttttaggtac aatctctaat gctaaaaatg
     1021 accttcttga tgaaattgca tatgagaagc aagccggaga tatgtataca caggtaattg
     1081 ctaaatgcta taaagcttat caagaggaat tacgtagaag tgaggccatg gatttcgatg
     1141 acttgattat gatgacactt cgattatttg atcagaataa agatgttttg gcctattacc
     1201 aacagaggta tcaatatatc catgtagacg agtatcaaga tactaaccac gctcaatacc
     1261 aattagttaa gttattagct tcgcgtttca aaaatatttg tgttgttggt gatgccgatc
     1321 aatccattta tggatggcgt ggagctgata tgcaaaatat tcttgatttt gaaaaggact
     1381 atccgcaagc caaagttgta ttattagaag aaaattatcg atcaactaag aaaatacttc
     1441 aagctgctaa taatgtgatc aatcataata aaaatcgccg tcctaaaaaa ttatggactc
     1501 aaaatgatga aggtgagcaa attgtatatc atagagctaa caacgagcaa gaagaagccg
     1561 tttttgtagc gtcaactatt gataacatcg ttcgagaaca aggaaaaaat ttcaaagatt
     1621 ttgccgttct ttatcgtacg aatgcgcaat ctcgtactat tgaggaagca cttttaaaat
     1681 ccaatattcc atatacaatg gttggtggaa caaaattcta tagtcgtaaa gaaattcgag
     1741 atgttattgc ttatctcaat atccttgcaa atacttctga taatatttct tttgagcgga
     1801 ttgtaaatga acctaaaaga ggggtggggc caggaacttt agaaaaaata cggtcatttg
     1861 cctatgaaca gaacatgtct cttcttgatt cctcttcaaa tgtcatgata tcgcccttaa
     1921 aagggaaggc tgctcaagct gtttgggatt tagctaatct gattttgact ctacgtagta
     1981 agctagatag tttaactgta acggagatta cagaaaacct actggataag acaggatatc
     2041 tagaagcact tcaagttcaa aatacattag aaagtcaagc gcgtattgaa aatattgaag
     2101 aattcttatc agtgactaaa aattttgacg ataacccaga gattacagta gagggtgaaa
     2161 ctggtttaga tcgtctatcg cgttttttaa atgatttagc tttaatagca gatactgatg
     2221 atagcgccac tgaaacggct gaagtcactt taatgacatt acatgctgcc aagggtctag
     2281 aatttccagt tgttttctta atcggtatgg aagaaggagt atttccttta tcgcgtgcaa
     2341 tagaggatgc tgatgaatta gaagaagagc gtcgtttagc ttatgtcggg attacgagag
     2401 cagaacaaat acttttcctt accaatgcta atacccgtac tttatttggt aaaactagtt
     2461 ataatagacc aacccgtttt atacgtgaga ttgatgatga gctgatacaa catcaagggt
     2521 tggctcgacc agttaattct tcatttggtg tgaaatattc aaaagaacag cctacacaat
     2581 ttggtcaagg aatgagtctt caacaagcgc ttcaggcacg taaaagtaat tcacaacctc
     2641 aagtaactgc ccaactccag gctcttaacg cgaacaatag ccatgagaca tcatgggaga
     2701 ttggtgatgt tgcaactcat aaaaaatggg gggacggtac ggtgcttgaa gtttcaggta
     2761 gcggaaagac tcaagagtta aaaataaatt ttccaggtat tgggttaaag aaattattag
     2821 cgagtgtcgc tcctattagt aaaaaggaaa actaactttc ttctattata tgcttaaata
     2881 atcagagact aagaattgta ttcttagtct ttttgcatca tttgaataga caaaataagt
     2941 tatcatttgg tttttactta ctaaaaaggt tgatattaaa gcgttttcca aaagtgacca
     3001 tactatttta aagaaagaag cgtttctctt gcattctttt ctaaaaagag tataataaca
     3061 atatcaatta aatatcaagt ggtcacttaa aaataaaagc atttaccaag ctttttactt
     3121 ggcagtcact ttataaagtt gtataatatt agtatagtta acaacttaat taattgccga
     3181 gtggtcactt tagaaactct tgttatttag ttaagaacaa aataagtttg tacactgaga
     3241 tgtacttagg aagaaaggta tttatcatgg ggttaatttg gtcattaatc gttggtgcta
     3301 ttattggtgc tattgcagga gcaatcacta acaaaggtgg ttcaatgggt tggatagcta
     3361 acatcttagc tggtttagtt ggttcatttg ttggtcaaag tttactagga acatgggggc
     3421 ctaagttagc agatatggct ttgattcctt caatcgtagg tgcgattatc gttatcattg
     3481 ttacttcatt tgtattaggc aaaaaatcat aactacataa ctagtagaat gattagtcaa
     3541 gaaagagaag gtatttatca tgagtttaat ttggtcatta atcgtaggtg ctattatcgg
     3601 tgctattgca ggggctgtta ctaacaaagg tggttcaatg ggttggatag ctaacatctt
     3661 agctggttta gttggttcat ttgttggtca aagtttacta ggaacatggg ggcctaaatt
     3721 agcaggtatg gctttgattc cttcaatcgt aggtgcgatt attgttgtca ttgttacttc
     3781 atttgtacta ggtaaaatga ataactagaa aaggagttac ctatgtcaaa aggactaaaa
     3841 agtttatata ctttgctagg tctaatctcc cttaccctac taggatttgt agctgttatc
     3901 agtaaacaac acatttatct accatctttt aattggttag attgggattt taatttgcca
     3961 tcaccgattg acgttggtat gtatcattat ttcttttggg gagcattagt gctgtttgta
     4021 atcgttttac tggctatatt ggtagttctg ttttatccaa gacgatacac agagtataaa
     4081 ctggctgata aaactggtaa attgatgtta aaaaaatctg caatagaggg atttgttaaa
     4141 acagaagtat taaaaactgg tttgatgaaa tctcctagtg tgacagctca tttatataag
     4201 aaaaaagtga aagttgatgt taaaggtctt ttaacttctc gtacaaatgt accggaacaa
     4261 cttgaacata ttcaaagtga tgttgaactt ggcttaaaag aattctttgg gttggaaaag
     4321 aaaatgaaca ctagagtatt tgttaagcaa gttgaagaag aaaatgttgg aaatgctaag
     4381 acgaataaaa gtcgtgtaga ataggagtct aatatgtcag aattcgtgag gaaatacaga
     4441 tatccactag gaggtgcagt tattgggctc gttttagctg caatgattgt aaccattgga
     4501 ttctttaaaa caattcttgc tcttgttatc attgtgctag gtgcttatgc tgggctttat
     4561 gtgcaaagaa caggtatgct tgaccaattc tttaataaga gaaaataaaa tttttcgttt
     4621 atatttattc tacattattt aaataaaaga aagaggaact attatgactg aaacttacat
     4681 caaaaacaca actaacaatt ctggcacgac agctgttcgt ggagaattaa ctttcgaaga
     4741 taaagtgatt gaaaaaattg ttggaattgc gattgagcat gtagatggtc ttttggctgt
     4801 aaatggtggt tttttctcaa atttaaaaaa tagcgttgta aattctgatt cagttacaga
     4861 tggcgtaaat gttgaagtgg gtaaaaagca agttgctgtt gatttggata ttgttgctga
     4921 ataccaaaaa catgttccaa ctatctttgc cgatatcaaa aaagttgttg aagctgaagt
     4981 gaaacgtatg acagaccttg aagtggttga agttaatgtt aatgttgttg atattaaaac
     5041 tcgtgctcaa catgaagaag attcagtaac tcttcaagat cgtgtaacaa gtgcagcaca
     5101 agcaacaggt gaatttgctt cgaatcaagt ttcaaacgtt aaaagtgccg taggtaacgg
     5161 tgttgataaa gtggaagaca tgaagtctga accacgtgta caataattga tttaagtata
     5221 gtttaaagta ttgttattag taggaggcta ttatgtctga agaaaaattt gatgcaaaag
     5281 ttgataaggt ttctggttca gtaaaagaat ctgttgggaa acttactggc gataaagaag
     5341 ttgaatctga aggtaaagtc gataaattga aaggtcatgc aaaagaaaaa cttgctgaca
     5401 ttaaagatac cattaagggt gcttcagaga gctttaagaa aaaggactag aaggtttgag
     5461 gtcatttatt tatggaaaat caaaaacaag atattaaaac ttcagtaact tatgaagaaa
     5521 aggtaattgc taaaattgtt ggacatgctt tagaatcagt agacggactc ttggctgtga
     5581 gtggaggttt cttctctaat ctaaaaaata gcgttgtaaa ttctgactca gtaactgatg
     5641 gtgtaaatgt tgaagttggt actaaagaag ttgcagttga tttagacatc gttgttgaat
     5701 acggcaagga tatcccagct atcgttgaaa gcattaaagc gattgtcagt caaaacgttg
     5761 aagtgatgac ccaccttaaa gttgttgaat tgaacgcaaa tgtcgtagat gttaagacta
     5821 aagctgaaca tgaagctgac tcagtgacag tacaagatcg tgtatctgat gcagcacaag
     5881 caacaggaaa ctttgcttct gaacaagctg gcaaagctaa agcagcaatt tcttctggag
     5941 cagagaaaac aaaagaagca gtatcaaatg gtactgaggc tgcaaaagaa aaaatctcag
     6001 aagctcgtac tagtgaatct taattcagta aactactttt tatgataaga cactcggagc
     6061 tgggagacca gctcctttta tatgatattt actaaagaca ttagatatta acaaaaaaga
     6121 tatatttgca atattttgct aattgtggtt taatatgtat gtttttatta ggataaggag
     6181 ggttggatgg tttctgaaca atctgaaatt gtaacatcta aatatcaaaa aatagcagta
     6241 gcggttgcac aaagaatcgc aaatggagat tatgaagttg gagagaagtt gaaatctaga
     6301 acaactattg cttcaacatt caacgtatca ccagaaacag cacgtaaggg tcttaatatt
     6361 ttagcagatt tacagatttt aactttaaag catggcagtg gagccattat tctttctaaa
     6421 gaaaaagcaa ttgcattctt aaaccaatat gaaacctctc attcagttgc tattttgaaa
     6481 ggtaaaatcc gtgataatat taaggctcag caacaagaaa tggaagagtt agctacttta
     6541 gttgatgatt ttttgcttca aacacgtgcg gttagtaaac aatacccact agcaccatat
     6601 gaaattattg tcagtgaaga ttctgaacac cttggtaagt caataggaga attaaatgta
     6661 tggcatcaaa ctggtgcgac tattgtggct attgaacacg aagggaaatt tattgtttct
     6721 ccagggcctt tctcagttat agagcaagga gatcatatct tttttgttgg tgatgaagat
     6781 gtttatgcac gtatgaaaac ctattttaac ttaaggatgg ggctttgaga gaatggattt
     6841 tgtctttgat atagatggaa ctctttgttt tgatggtatg agtctatcta aagaaataca
     6901 aggaatacta gagagagccc agattgatta tggtcataga gtcacttttg ctacggcgcg
     6961 atcttataga gacactatag gtatccttgg cgacaagcta tctttatcaa aaattattgg
     7021 tttaaatggt gcgactttac atgaaaatgg tcacttagtg gatagttact atttacaaag
     7081 tgactttttt agtactatta tttcatattg tcatcgctat cagattccat attttgttga
     7141 tgaggtcttt aattacgcta cttatcaggc ttctaaaatt ccctttattg cttatgttga
     7201 tcctcaaaaa aggggagagc ttctagaagt ttctaaaatt gagaaaccga taaaaatggt
     7261 gttgtatttt ggtgatcagc tgagaagggc agaccaaatg ttagcgaaat taaatcgttt
     7321 tggacttagt agtcactttt tccacgaatt tgaaaaatgt ttgtacataa atcccattgc
     7381 agttgacaag ggaaaagcca ctaagaaatt atttgggaat cgttttatag cttttggcaa
     7441 tgataaaaat gatatttcta tgtttgatgc tgcgcattat agtgttcaag taggggattt
     7501 tgatgaactg actccttacg ctaatttacg tgtatcaaga gaatctgtac atgaaggaat
     7561 tacaatgctt tttgaaaaat acaaggggat tacgtaatgg atttattcct aagtttagag
     7621 aggaaattta aagctgcaag tgacaaagaa gtttctaagc aacaggagac ttatttacgc
     7681 catcatttta agtgctatgg tattaaatca cctgagcgac gtatgctcta caaagagctt
     7741 ataaaggccg ctaaaagaca agcaaaaatt gattggcaat tgcttgataa atgttggcaa
     7801 agtgactatc gtgaatatca tcattttgta cttgattatc tattagctat gagtcaattt
     7861 ctcacctata atgattgctc tagactagag ttttacgctc gtcatcaaca gtggtgggat
     7921 agtattgatg ttctgactaa gatttttggg aacttatcct taaaagatga caaggttatg
     7981 aatttactgt cagaatggtc acttgatcaa gatttttgga tgaggcgact agctattgag
     8041 catcaactag gatttaaaga aaaaacaaat actgatattt tgtcactttt tattttaaga
     8101 aatacaggaa gtcaagaatt ttttatcaat aaggcgattg gctgggcgct tagggattat
     8161 tcaaagtata ataaagtatg ggtaaaggac tttatcagta atcattatga tgagttgtcg
     8221 actttgagca tacgcgaagg aagtaagtac ctctaattat cagttcaatt attcgaaaaa
     8281 gaagaagctt catagtaaaa taaaggtaat tgataaggag ataaacttat gacgacattt
     8341 cttggaaatc ctgttacttt tacaggaaaa cagctacaag ttggggatat tgcaaaagat
     8401 tttttactaa tagctacaga cttatctcaa aaatctttaa aagattttga agggaaaaag
     8461 aaagttatta gcgttgttcc ttctattgat acaggtattt gttcaaaaca aacacgtact
     8521 tttaatgagg aattatcaga gctagataac acagtggtta ttactgtttc tatggacctt
     8581 ccatttgctc agaagcgttg gtgcagcgct aaaggtcttg ataacgtgat tttattatca
     8641 gatttttatg atcattcgtt tggtcaagag tacgctcttt tgatgaatga gtggcatttg
     8701 ttaacgcgtg cagttcttat tttggatgag cataataagg tgacttacac cgaatacgtt
     8761 gacaatgtta attcagatgt tgactacgaa gcggcgatta atgctgcaaa gatattacca
     8821 taaataata
//
LOCUS       DATRME010000076         8790 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRME010000076 DATRME010000000
VERSION     DATRME010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1677
                     /gene="fbsB"
                     /locus_tag="VBC23_001724"
     CDS             <1..1677
                     /gene="fbsB"
                     /locus_tag="VBC23_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO6843653.1"
                     /translation="EEFQDPLYSDAREELISFIFPYEKFLINNRDITKLTVNQYEAIV
                     NRMSVALQKFSKNIFEKQKVNKDLIPIAFWIEKSYRTVGTNEIAASVGIQGGFYQNFH
                     DYYNYSYLLNSLWYEGNVKEVVKDYENTIRQILSKKHEIEKILNQSTSDISIDDDDYE
                     KGNKELLREKLNIILNLSKRDYRVTPYYEVNKLHTGLILLEDVPNLKIAKDKLFSLEN
                     SLKEYKGEKVNYEELRFNTEPLTSYLENKEKFLVPNIPYKNKLILREEDKYSFEDDEE
                     EFGNELLSYNKLKNEVLPVNITTSTILKPFEQKKIVEDFNPYSNLDNLEIKKIRLNGS
                     QKQKVEQEKTKSPTPQKEAVKEQTEQKISGKTQNVEKKSETVATPQQSSTAQTSVQQP
                     APVQPVVQESKASQEEINAAHDAISAYKSTVNIANTAGVTTAEMTSLINTQTSNLSDV
                     EKALGNNKVNNGAVNVLREDTSRLENMIWNRAYQAIEEFNVARNTYNNQMKSETVPVD
                     NDIEAILAGSQAKISHLDNRIGARHMDQAFVASLLEVTEMSKSISSRIKE"
     gene            1744..2289
                     /locus_tag="VBC23_001725"
     CDS             1744..2289
                     /locus_tag="VBC23_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843654.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      2440..2529
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2685..3251
                     /gene="thiW"
                     /locus_tag="VBC23_001726"
     CDS             2685..3251
                     /gene="thiW"
                     /locus_tag="VBC23_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6843655.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            3248..3802
                     /locus_tag="VBC23_001727"
     CDS             3248..3802
                     /locus_tag="VBC23_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6843656.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3806..5092
                     /locus_tag="VBC23_001728"
     CDS             3806..5092
                     /locus_tag="VBC23_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6843657.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            5080..5748
                     /locus_tag="VBC23_001729"
     CDS             5080..5748
                     /locus_tag="VBC23_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6843658.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            5832..6512
                     /gene="tenA"
                     /locus_tag="VBC23_001730"
     CDS             5832..6512
                     /gene="tenA"
                     /locus_tag="VBC23_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6843659.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            6514..7311
                     /gene="thiD"
                     /locus_tag="VBC23_001731"
     CDS             6514..7311
                     /gene="thiD"
                     /locus_tag="VBC23_001731"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6843660.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            7313..8083
                     /gene="thiM"
                     /locus_tag="VBC23_001732"
     CDS             7313..8083
                     /gene="thiM"
                     /locus_tag="VBC23_001732"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6843661.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            8097..8768
                     /locus_tag="VBC23_001733"
     CDS             8097..8768
                     /locus_tag="VBC23_001733"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6843662.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
BASE COUNT         2912 a         1273 c         1754 g         2851 t
ORIGIN      
        1 gaagaatttc aagatccttt atatagtgat gcgcgcgaag aattgataag ttttattttt
       61 ccgtatgaga aatttttaat taacaatcgt gacataacta aattaacagt taatcagtat
      121 gaagcgattg tgaatagaat gagtgttgct ttacaaaaat tttcaaagaa tatttttgag
      181 aaacagaaag taaataaaga tttaatccct attgcgtttt ggattgagaa aagttacaga
      241 actgttggaa cgaatgaaat cgccgcttct gtaggcattc aaggaggatt ttatcaaaac
      301 ttccatgatt attataatta ttcatatcta ttaaattctt tatggtatga aggaaatgta
      361 aaagaagtag ttaaggatta tgaaaacact attcgtcaaa tactatctaa aaagcatgag
      421 attgaaaaaa ttcttaatca gagcacttct gatatctcta tagatgatga tgattacgaa
      481 aaaggaaata aagaattgct aagggaaaaa ttaaatatta ttctaaatct ttcaaagaga
      541 gattacagag taactccata ctatgaagtg aataaactac atacagggct tattttattg
      601 gaggatgtcc ctaatttaaa gattgctaag gataagttgt tttcattaga gaattcttta
      661 aaggaataca aaggagagaa agttaattat gaggaactaa gattcaatac ggaaccttta
      721 actagttact tagaaaataa agaaaaattt ttagtcccca atattccata taaaaataaa
      781 ttaattttaa gggaagaaga taaatatagt tttgaagatg atgaagaaga gtttggaaat
      841 gaacttctaa gttacaataa gcttaagaat gaagttttac ctgttaatat tacaacttct
      901 actatattaa aaccgtttga acagaagaaa attgtggaag attttaatcc ttattctaat
      961 ttagacaatt tagaaataaa aaaaataagg ttgaatggct cccaaaaaca aaaagtagaa
     1021 caggaaaaaa ctaaatcgcc aactcctcaa aaagaggctg taaaagaaca aactgagcaa
     1081 aaaatatctg ggaagactca aaacgtagaa aagaaatctg aaactgtggc aactccacaa
     1141 caaagttcaa ctgcgcaaac ttctgtccaa cagccagctc cggttcaacc agttgttcaa
     1201 gaatctaaag cttctcaaga ggagattaat gcagcacatg atgccatttc agcgtataaa
     1261 tcaacagtca atattgctaa tacagccggt gtaacaactg cggaaatgac ctcgctcatt
     1321 aatactcaaa cttctaatct ttctgatgtt gagaaagctt tagggaataa taaggttaat
     1381 aatggtgcag tcaatgtatt gagagaagat acatctcgtc ttgagaatat gatttggaat
     1441 cgtgcttacc aagctattga agaattcaac gtcgctcgta atacttataa taaccaaatg
     1501 aagtcagaaa cagttccagt tgataatgat attgaagcta ttttagcagg ttctcaagct
     1561 aaaattagcc atttggacaa tcgtatcgga gcgcgccaca tggatcaagc ttttgtagct
     1621 agtttattag aagttactga gatgagtaaa tcaatctcat cgcgtataaa agaatagaca
     1681 ctgctatcaa ggcgatctta aacttttgta ttgagctaac ctaaaagata gaaagagact
     1741 aatatgaaaa aaataacaac tttaatctta gctagtagct tattactagt tgcaacgaca
     1801 tcggttaaag ctgatgataa ctttgaagta ccaacgcgtt atgttaaaat gagtgaaaaa
     1861 tcaaaagcat tttatcaaag attacaagaa aaacaacgta aaacacatac tactgtgaag
     1921 acttttaata attcagaaat aaggcatcaa ctacctctta aacaagaaaa ggctaggaat
     1981 gatatctaca atttaggcat tcttatttct caggagtcta aagggttcat ccaacgtatt
     2041 gataatgcct attctttgga aaatgtctca gatattgtta atgaagctca ggctttgtat
     2101 aaacgcaact atgatttatt tgaaaaaatc aaatctacac gtgataaggt tcaagtctta
     2161 cttgcatcgc atcaaggtaa tacagattta aaaaactttt atgctgagtt ggacgatatg
     2221 tatgaacatg tttatctcaa cgaaagtaga gtggaggcga taaacagaaa tatccaaaaa
     2281 tataattagt ttctaaacta acaaacattc ctaaatataa gacattaaac cctacttatt
     2341 gattagtgag tagggtttta ctgttttaaa tagctttctg ctcagaatgt aagccttgtc
     2401 atttcaaagg aactatgtta ttattcttaa gtaaattaaa taggacattt ggggtgcgta
     2461 acagctgaga ttatacccat tgaacttgat acagttagta ctgtcgaagg gaaatgtgtg
     2521 ccattacaag ttttttgtaa tggaaaaggg cgtagtgtct tcttaggaga cagctagctc
     2581 tagttaatca gataataaac tccgtttgct tgcagatgga gttttttgtc gtcaaaattt
     2641 tttataggtt gttgcccctt atctgtcata gagaaggagt tatgatgact aatcataaaa
     2701 caaaaaaatt aacggtttta gcaattatga ttgctttaga tgtttttcta acgcccattt
     2761 ttagaataga aggcatggcc tttatgtcta gtgtggttaa tattatcgct ggagttataa
     2821 tgggaccaat ctatgcatta gttatggcac ttgtaacggc ttttattcgt attttaactc
     2881 aagcaggagt agcatctgtt gcacctttag ctattctagg agctggtcca ggagctttct
     2941 tagcgggttg gttttatcgg ttttatgcga aagactggat gtcttgggtg ggtgagttta
     3001 taggaacagg gataattgga gcacttttat cagctccaat tatggtctgg ttttggactt
     3061 ggacagcgga tggaaatcac gaagtgttag ctaaagcatc tgcagctcaa tccatttttc
     3121 tttttatgcc aaggtttata ggggcgacta taattggcgg tgttattgct ttgttgctac
     3181 ttcagggatt aaagcgggtt cctgtattta tgacaataca agcattattt aaaggagaaa
     3241 aagtaaaatg aaattgggtt taaaagatgt attattgatt gctttattag cggttgttct
     3301 gggcgttgtt tactttggag caggctatat ttcaaatgct tttgtacctt ttgtaggtcc
     3361 tatagcgcat gaagttattt atggtatttg gtttgtggca ggtccaatgg ctctttatat
     3421 attgaggaaa ccaggaaccg ctattgtagc agaattgtta gctgctttga tagaggtttt
     3481 aattggatca atttatggac caagtgtact cgttattggt acccttcaag gtttagggtc
     3541 agaattaggc tttacgctgt ttaggtatca taattacaag ttgccagctt ttatattgtc
     3601 tgctattttg acatcaattt ttagttttgc atggagtttt tatgccaatg gacttagtgc
     3661 attttcattt tcatataaca ttctgatgct tatagtaaga actgtttcat ctattatttt
     3721 cttcttatta acaaagaata tatgtgacca attgcataga agtggtgtac tgaatgctta
     3781 tgggattcat gaagggaagt agcctatgct atctgttgaa aaattagctt acacccatgg
     3841 cgatagtcat tatttatttg atgaagtgac ttttagtctt aacccaggag aacgaatttt
     3901 aattagtggc tattcagggt gtggtaagag tacattggct cttttactat cagggttaaa
     3961 agaaagtggg aaaggtcaag ttttattaaa cggctcatta atagaaccaa gcgatgtggg
     4021 ttttcttttt caaaatcctg atttgcaatt ttgtatggat actgtggcgc atgagttgta
     4081 ttttatttta gaaaatttac aaatagaacc ggagcaaatg caagatagaa gtgagtttgt
     4141 attggcacaa gttggtttaa aaggatttca gaatcgtttg atttatactt tatctcaagg
     4201 tgaaaaacag cgattagcat tggcgactat ttttttaaaa tctcctaaac ttattatttt
     4261 agatgaagct tttgcgaatt tggatcaaga gagtgccagt caactattac aattagttct
     4321 taattatcaa gcaaacaatc aatcaatgct gattgtaatc gatcatttga ttacttatta
     4381 tcaagatata atggatcact atttttggtt ggaaaaatgg ttgataaggg tcgattttga
     4441 ttatatgctc aatcgtttga atgtcttcga attagaaaag aaaagtcata atacaggtga
     4501 caaactgtta tcaattaaag atttccaagt taaactttca aaaaacaaat tcatctctta
     4561 ccttgatttt gatttagcaa gtggggagag gttgtgctta gatggaccaa gtggcgtggg
     4621 gaagtctagt ttctttatgg gattgttagg gctctatagg actaaaggaa agaaacaatt
     4681 tactcataga aagcaaattc ctataagctt tttatttcaa aatcccttag atcaatttat
     4741 tttttcaaca gtttatgacg aaatttttca ggtttgcaag gatagtaaca aagcgaggga
     4801 tattttggaa accataaatc tttgggataa aaaacagttt tcaccatttc aactaagtca
     4861 aggccaacag aggcgattag ccattggcag tattttagct agtgatagta aacttctatt
     4921 attagatgaa ccaacttatg gacaagatgc ctatcacgct aatatgatta cgactcttct
     4981 tttgagttat tgtcacaaaa atcattgtgg ggttattttt actagccatg atcctcactt
     5041 aaaagagtgt tttgcaacac gtttcttgga ggtgtcatct tgttcaattt gaaaaactat
     5101 catccagcta tcaccttcct atgtttatta ataccaacct tttcgtttag tttcacattg
     5161 aggctaagaa cgtctttgtt atttctaatt atagtagtaa cactgcaatg cttcgtaaag
     5221 gtttctttaa aaacttgggc gaaggtcaat ttgatctcat ttgtgatggg actctcttta
     5281 tttttaggga cgtatttttg ggggaaatta ccccatcaat tcgtcttagc tagtctagtg
     5341 gcctgtcgtc cgcttatttt tatgaacgtg ggtttgttat ttcatgcatc acattctaat
     5401 tatgatttta tagaatctct ttatcaaact ttcaaagtgc ctagtcattt tgcatatggt
     5461 atttttgcgg tctttaatct tttaccatta attaaattac aataccaacg taatcgactc
     5521 gcattccgac tgaagaatca ggtaacttgg gcattatcgc ctagactaat attgtcagtt
     5581 ttattaaaga cgatatattg ggtagaacag ttagagttag caatgctttc taaaggtttt
     5641 gaagtgggta aagagcgaac acatgcttca acttatcccg ttcgttttcg agattatagt
     5701 ttacttggta tgagtatttt attggctata ggaatgattt ttaaataagt acaattagtt
     5761 tagtaataca attgtgcatt tatatagagt gagcatgttc tgctcatgag atcataacaa
     5821 aggagattac tatgttaaaa gatgagatga ttgctaaagg aacaaaatat atacaatcca
     5881 tctatcaaga tccatttatt caaggaattg taaaagggcg tttagaccat gacgtaatct
     5941 gccattattt acaggcagat aatatctacc ttgggaaatt tgctgatatt tatgccctgt
     6001 gcttggcaaa atctgataat ttacgagata aacaattttt ccttgaacaa gttgacttta
     6061 cattaaatcg ggagttagca gatggtgaag gtccacatca agccttggca gcttatacta
     6121 atcgatctta tcaggatatt atcgaaaagg ctgtatggta cccgtcagca gatcattaca
     6181 ttaaacatat gtattttcat ttttacgaaa atggtattgc tggtgcttta gctgcaatga
     6241 gcccatgtcc atggatttac catcaattgg ctaaaaaaat aattgaggag aaccagtttt
     6301 tgaatggtaa cccttttaat aattggataa ctttttatgc taacgataca gttgaagagt
     6361 taatggaaaa ctattttcgt atgatggatt attatgcgca gaatttgtca aaagagaagc
     6421 aagctgattt ggtagatgct tttgtgaaat cttgtcagca tgagagacgt ttttttcaaa
     6481 tggcaataaa tcaagaaaaa tgggaggatt aatatgactt atttagcacc agttttaact
     6541 attgcaggaa ctgatcctag cggtggagca gggattatgg cagatttaaa gacatttcaa
     6601 gctcgtcgga cttacgggat ggctgttgtg actagtgttg ttgctcagaa cacttgtggg
     6661 gttagaggcg ttcagcatat agaaacagct atcattgatc aacaactagc ttgtgtttat
     6721 gatgatatta agccaaaggc ggtcaaaaca ggaatgttag cggagaggga aacgattagt
     6781 ttagtcgctt catatttaaa aaaatatccg caaccttatg tattagatcc tgttatggtg
     6841 gcaactagcg gacatcgttt aattgattca gatgccgtag aagcattaaa agaagactta
     6901 ttgccattag caactattat cacaccaaat ttaccagaag ctgaggtttt agtgggatat
     6961 gacttatcag atgaagtaag tattattaaa gcaggttacg atattcaaaa acaatattct
     7021 gttagaaatg ttcttataaa gggaggacat ctagatggtt tggctaaaga ttatttattt
     7081 ttggaaaaag aagggttgat aactcttagt aatcaacgta tcaatactat tcatactcat
     7141 gggactgggt gtacatttgc agcagtcgtt gcagcggagt tagctaaagg gcaatcaatt
     7201 ctaaacgcgg taagtaccgc taaatcattt attacttcag ctatcgaaac agcgcctgag
     7261 ttgggtcttg gaaacggtcc tgtcaatcat actagctatc aaggagacta aaatgtattt
     7321 agaacaacta aaagaggtaa atcctttaac catttgtatc actaataatg tggtcaaaaa
     7381 ctttacagcc aatggtttat tagctttagg agcttctccg gctatgagcg agtgcataga
     7441 ggacttagaa gacttgttaa aagttgcaga tgcattatta attaatatag gaacactaac
     7501 aaaagaaagt tggcaacttt accaagaagc aatcaaaata gcaaataaaa accaagttcc
     7561 agttgtatta gatcctgtag cggcaggagc aagtcgtttt cgattagaag tgtcacttga
     7621 tttattgaag aattatccta ttagtttact tagaggaaat ggtagtgaaa tcgctgcttt
     7681 ggttggtgaa aaacaagcgt ctaaaggggc agatggggga aaagtggctg atttagaatc
     7741 aattgctgta aaagccaatc aagtttttga tgtgccagtg gttgtcacag gagaaactga
     7801 tgctatcgct gttagaggag aagttcgctt attacaaaat ggtagtcctt taatgccact
     7861 tgttacagga actggatgtc tattaggggc agtattggct gcttttattg gtagttctga
     7921 cagaagcgat gatcttgctt gtctgacaga ggccatgact gtttataatg ttgctggaga
     7981 aattgcagag aaagtggcaa aaggtaaggg agtgggaagt ttccaagttg catttttaga
     8041 tgccctttct cagatgaaat cagaaatgat tatggacaaa taggcggtgt cagtagatga
     8101 aagatacatt gaaattatat tttgtttgtg ggactgtgga ttgctctagg aagaatatat
     8161 tgactgttgt tgaagaagcc ttacaagcag gtataactct atttcaattt agagaaaaag
     8221 gttttacagc attacaaggt aaggaaaaaa ttgctatggc taagcaactc caaatacttt
     8281 gtaagcaata tcaagttcct tttattattg atgatgacat tgacttagtt gagttaattg
     8341 atgctgatgg tcttcatatt ggacaaaatg atttacctgt tgatgaggca cggagacgtt
     8401 taccagataa aatcatcggt ttatctgtga gtacaatggc tgaatatcag aaatcacaat
     8461 tatctgttgt tgattacatc ggaattggtc cttttaatcc gacacaatca aaagcagatg
     8521 ctaagcctgc tgttggcaat aggacaacaa aggcagttag agaaattaat caagatattc
     8581 caatcgttgc aataggcgga atcactagtg attttgttca tgatattata gaatcaggcg
     8641 cagatgggat tgctgttata tcggctattt caaaagcaaa tcatattgta gatgcaacca
     8701 gacaattgcg ctatgaagtt gaaaaagctc tggttaatag gcaaaaacac agtgatgtaa
     8761 tcaaataagg ctacatcgtt aattataaga
//
LOCUS       DATRME010000077         8773 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRME010000077 DATRME010000000
VERSION     DATRME010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            93..767
                     /locus_tag="VBC23_001734"
     CDS             93..767
                     /locus_tag="VBC23_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6843663.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILSKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIETH"
     gene            937..3189
                     /gene="gshAB"
                     /locus_tag="VBC23_001735"
     CDS             937..3189
                     /gene="gshAB"
                     /locus_tag="VBC23_001735"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6843664.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            3313..4131
                     /locus_tag="VBC23_001736"
     CDS             3313..4131
                     /locus_tag="VBC23_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843665.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            4128..5384
                     /locus_tag="VBC23_001737"
     CDS             4128..5384
                     /locus_tag="VBC23_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6843666.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            5524..6399
                     /gene="hslO"
                     /locus_tag="VBC23_001738"
     CDS             5524..6399
                     /gene="hslO"
                     /locus_tag="VBC23_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6843667.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            6383..7360
                     /gene="dusB"
                     /locus_tag="VBC23_001739"
     CDS             6383..7360
                     /gene="dusB"
                     /locus_tag="VBC23_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6843668.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            7481..8122
                     /locus_tag="VBC23_001740"
     CDS             7481..8122
                     /locus_tag="VBC23_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6843669.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            8136..8627
                     /locus_tag="VBC23_001741"
     CDS             8136..8627
                     /locus_tag="VBC23_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6843670.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
BASE COUNT         3019 a         1564 c         1532 g         2658 t
ORIGIN      
        1 ttattacatt aatcttttaa aatagtctga tattgtcttt taaccataaa tatattacta
       61 taatagtaag attagcatag aggaactacg atatggataa agaaaaatta gactattgga
      121 agacgataat aacgtttctc cataatgttt taggtgataa ttacgaaatt gttttgcatg
      181 tagtagatga aaatgatatc tatattggtg agttggttaa tagtcacatt agtggacgaa
      241 ccataagttc acctctaacg actttcgccc ttgatttaat caagaataaa gtttataagg
      301 aaaaagattt tgttactaac tataaggcga ttgtcagccc tctcaataaa gaagttcgtg
      361 gatctacttt cttcatcaag aatgctcaaa atgaacttga agggatgtta tgtatcaacc
      421 ttgatatttc tgcataccaa aatattgctt tggatattct agacttagtt aatttaaatg
      481 ttaacaaaat actatctaag tcccctcaaa aaatttctct ccctcaacag gaggaacctg
      541 tagaagttct atcaggcaat attcaggata ttattagtga gattgttgat cctagtcttt
      601 taaatcagaa tattcattta agtcaagaag ttaaggtaga aattgtttct aaacttcacg
      661 aaaaaggtgt attccaatta aaaggtgccg tttcaaaggt cgctgaagtt ctcaacatct
      721 ccgaaccaag cgtctatcgt tatcttaaaa aaattgaaac tcattaactc taaagctctt
      781 tttaacttat agattaataa gattggactc aaaagttcaa tcttttattt tttatgacac
      841 cagtataaga ttattttagt tttctgtgcc gtaaaactta gtttttatta tttttttgat
      901 aaactagtat catctgttac taggaggaat gttgtcgtga ttatcgatca actgttacaa
      961 agaagccact ctcatctacc gattctacaa gctacatttg gcttagagag agaaagcctt
     1021 cgtattcacc aacccactca aagggttgct caaacacccc atcccaaaac attagggagt
     1081 cgtaactatc atccttatat ccaaacggat tatagtgaac ctcaattaga actcattaca
     1141 cctattgcaa aggatagcca agaagccatt cgatttttga aagctataag tgatgtcgct
     1201 ggacgatcta tcaaccacga tgaatactta tggccgcttt ctatgccacc caaagtgagg
     1261 gaagaagaca tacagattgc ccaattagag gatgcttttg aatatgatta tcgcaaatat
     1321 ttggagaaaa cttatggaaa actaatacaa tccatctctg gaattcacta taatctcggt
     1381 ttaggtcaag agttattaac ttctctcttt gaattgagtc aagcagataa tgctattgat
     1441 tttcagaacc agctttacat gaaattgtct caaaattttt tacgttaccg ttggttacta
     1501 acttatctat acggagcgag cccagtagct gaagaagact ttttaaatca gaaacttaat
     1561 aaccctgtcc ggtcacttag aaatagtcac ttaggctatg tgaatcataa agatattcgt
     1621 atttcttata ctagcttgaa ggattatgtc aacgatctag aaaatgctgt aaaaagtggg
     1681 caattgattg ctgagaaaga attttattca cctgttcgtc tccgtggtag taagtcctgt
     1741 cgtaattatt tagaaaaagg aattacatat ttagaatttc gcacttttga tctcaaccca
     1801 tttagcccaa tcggtataac gcaggaaact gttgatactg ttcacctttt cttattggcg
     1861 cttttgtgga tagattctag tagtcatatt gaccaagata tcaaggaagc caatagatta
     1921 aatgacctta tagcactcag tcatccatta gaaaaattac ctaatcaagc accagtttct
     1981 gacttagtgg atgctatgca atctgttatc caacatttta acttaccacc ttactatcaa
     2041 gacctattgg aatctgtaaa aaggcaaatt cagtcaccta aattaacggt agctggtcaa
     2101 cttttagaaa tgattgaagg actttcctta gaaacatttg gacaaagaca aggacaaatt
     2161 tatcacgatt atgcttggga agctccctac gccttaaagg gatatgaaac gatggaactt
     2221 tctacccaat tgctactatt tgacgttatc caaaaaggag tcaacttcga agtactggat
     2281 gaacaagatc aattcctaaa attatggcac aatagtcata ttgagtacgt taaaaatggt
     2341 aatatgacct caaaagataa ctatattgta ccgctcgcta tggctaacaa agttgttaca
     2401 aaaaaaatcc tagatgaaaa gcatttccca actccttttg gagatgaatt tactgaccgt
     2461 aaagaggcac ttaactattt ttcacaaatc caagataaac caatcgtcgt taagccaaaa
     2521 tctacaaact ttggtttagg catttctatt tttaaaacat cagctaactt agcttcttat
     2581 gaaaaagcaa ttgacattgc ttttgccgaa gatagcgcta ttcttgtgga agaatatatt
     2641 gagggtaccg aataccgttt cttcgtcctt gagggggact gtattgctgt attgcttagg
     2701 gtagcagcta atgtcgttgg agatggtatt catactatta gtcagctagt taaacttaaa
     2761 aaccaaaacc ctctcagagg ctatgatcat cgctctcctc tagaggttat tgagctcgga
     2821 gaagtagaac agttaatgtt ggaacaacaa ggatacactg ttaacagtat ccctccagaa
     2881 ggaacaaaaa tagaacttcg ccgtaattct aatatttcca ctggcggtga ctctatagat
     2941 gtaacaaata caatggatcc tacatataaa caactagcag ctgagatggc agaggctatg
     3001 ggggcttggg tttgtggagt tgatttaatt attcctaatg ccactcaagc atactcaaaa
     3061 gataagaaaa atgctacttg cattgaacta aactttaacc cattgatgta tatgcacacc
     3121 tactgtcaag aggggcctgg tcaatctatt acacctcgaa ttttggctaa actgttccca
     3181 gaattataac taaaaagagt tccttaggct aagcttaagg aatttttggt taaaataaag
     3241 ttatcccttg tcatcactag ttcatttaag ataaaatatt aacaactaca gaaagttagg
     3301 ttaaatatta ttatggttaa aaaacgctat tccaaaaatt ctcataatct aataacatta
     3361 cttggtattg ttgttcttgc ttccctgatt tcagactttt ggagtgaggt gattgctact
     3421 ctgttaatta ttggtggtgg ttattgtgcc tattacgttt atgataaaaa gcgtcttaaa
     3481 tgttttacat caaaccaacg tattgaagct ttaaaatccg atattaaaga gactgaccaa
     3541 gacattagac acttggaaat tttaaaaaaa gacaatcgtt ctaaggaata tatcaaatta
     3601 gcacatcaaa tattacctca attggaccta attcgtaatg aagcaaacca actacaaaaa
     3661 gccattgagc ctaatatcta taaacgtatc accaaaaaag caaatacatt tagtaatgag
     3721 attaacgaac aattaataaa gctacatgcc tctccagaac tagaacctat ttctgatcag
     3781 gaagatgaaa tgatacgcat tgctccggaa ttaaaaccgt tctatcacaa tattcaagat
     3841 gatcactatg ctatcttaaa aaagattgaa gaggcagata acaaagctga attagcagca
     3901 attcatcaag ccaatatgaa acgttttaca gatgttctgg ctggctacat cagaatcaaa
     3961 caatctccta aaaattttaa caatgctaag gaacgcctag aacaagcatt acaagcaatt
     4021 aaaaaattta atttagattt agatgaaacg ctgcgccaat tgaatgaaag tgatatgaaa
     4081 gattttgacg ttagcctaag aatgatgcaa gacgaaagga acaataaatg agcgatacct
     4141 ttaattttga tattgaccaa attgcagaca atgctatcac taaaacagat aaaacaacag
     4201 aaattatttc caaccagaca acaagccaaa ctgggcaaat tgcctttttt gaaaaactaa
     4261 caccagcaca aaagtctgct atctctgaaa aaacaccagc tttggtagat acttttgtcg
     4321 gtgaccaaaa tgcgctcctt gattttggac aatccgcagt agaaggcgtt aatactactg
     4381 ttaatcatat cttgtctgag cagaaaaaaa ttcaaattcc tcaagttgat gatttactaa
     4441 aaaatgctaa tcgcgaacta aatggattta ttgccaaata taaagatgct actccggcag
     4501 aattagagaa aaaaccaaac ttgattcaaa aattattcaa acaaagcaag acctcgctac
     4561 aggaatttta ttttgactca caaaacatcg agcaaaaaat ggatatgatg gcagcaaatg
     4621 ttgtcaaaca agaagatact ttggcaagaa atatcgtctc tgctgaaatg ctcattgaag
     4681 ataatactaa atctattgaa aatttggttg gagttattgc ttttattgaa tcgagtcaag
     4741 ccgaggctgc caatcgtgca agccacttac aacaagaaat tctagcatta gatagccaaa
     4801 cgtccgaata tcaaattaaa agtaaccaat tagcccgaat gactgaagtt atcaataccc
     4861 tcgaacagca acatacggaa tatgtcagcc gtctctacgt tgcatgggca acaacaccac
     4921 agatgcgaaa cttggtcaaa gtatcgtcag atatgcgtca gaaacttggt atgttacgtc
     4981 gaaataccat tccaacaatg aaactctcaa tcgctcagtt aggcatgatg caacaatctg
     5041 tcaaatccgg tgtcactgct gatgctattg tcaacgctaa taatgcagca ttgcaaatgc
     5101 tggctgaaac tagtaaagaa gcgattccga tgttagagaa gaccgcacaa agccccactg
     5161 tttctattaa atctgtcact gcattagctg aaagcttagt ggctcaaaat aatggtatta
     5221 tcgctgccat agacaaagga cgtaaggaac gtgcccaatt agaatctgct gttattaaat
     5281 cggctgaaac aatcaatgat tctgtcaaaa ttcgtgataa aaaaatagtt gaagccttac
     5341 tcaacgaagg taaatctacc caagaaaaag ttgatgagtc ttaacttact atcatatcca
     5401 ctatataaaa acgatactgt aaaacacagt atcgttttta tatcattaat ttataccgtt
     5461 ttcatctata ttttcctgac tatctccaat gcaaaataag ccttttatgc tataatatga
     5521 cttatggata aaattataaa atcaatttca acttccggtt ctttccgggc ctatgtcctt
     5581 gattgtacag aaactgtaag aacagctcaa gaaaaacatc agacactttc aagttctact
     5641 gtagctcttg ggagaacatt aattgcaaat caaattttag cagcaaatca aaaaggaaat
     5701 agtaaagtaa ctgtcaaggt tattggagat agctcttttg gtcatattat ttctgtagct
     5761 gatactaaag gaaatgttaa aggttacatc caaaatacgg gtgttgatat caagaaaaca
     5821 gctactggag aggtcttagt tggacctttc atggggaatg gacactttgt cgttattacc
     5881 gattacggaa ctgggcaacc ttacacttca acaactccac taattacagg agaaattgga
     5941 gaagattttg cttattattt aaccgaaagt gagcaaacac cttctgccgt cggtttaaat
     6001 gtcctcttag atgacgagga taaggttaaa gttgcaggtg gattcatgct tcaggtgcta
     6061 ccaggtgctt ctgacgaaga aatttctcgc tatgaaaaac gcattcagga gatgccttct
     6121 atttcaagtc tgctagagtc tgaaaatcat atagaatctc tcctttcagc tatttacgga
     6181 gaagacgact acaagcgctt atcggaagac tctttagcct tctattgtga ctgttcaaaa
     6241 gaacgttttg aagccgctct attgacttta ggaactaaag aattgcaagc catgaaggat
     6301 gaagataaag gtgttgaaat tacttgtcaa ttttgcaacc agacttacta tttcacagaa
     6361 gaagatttgg agaagataat taatgactca attaaatagt tcatttatga ttgggaaggt
     6421 tgaaattcct catcgtaccg tactagcacc gatggcaggt attactaatt ctgcttttcg
     6481 tactatcgct aaagaatttg gtgctggtct cgttgttatg gaaatgattt ctgagaaagg
     6541 gttactttat aacaacgaaa agaccttaca tatgctccat atcgatgaaa atgaacatcc
     6601 tatgtcaatt caactatttg gtggcgatgc agaaggctta aaacgtgcag ctgactttat
     6661 ccaaaataat acgaaagcag atatcgtaga catcaatatg ggatgtccag ttaacaaagt
     6721 tgttaaaaac gaagctggag ctaaatggtt acgtgaccct gaaaaaattt atcatattgt
     6781 taaagaggta acgtctgtac ttgacattcc attaacagta aaaatgcgta caggatggtc
     6841 tgactcatct aatgctgtag aaaatgcact cgctgccgaa tctgctggtg tatctgcact
     6901 tgctatgcac ggtcgaacac gtgaacaaat gtatactggc acgtgtgacc atgaaacact
     6961 tggcaaggtc gctaaagctg ttactagtat tccttttatt gccaatggtg acattcgtac
     7021 tgttcacgat gccaaattta tgattgaaga gattggagct gacgctataa tggttggacg
     7081 tggtgctcgt agcaatcctt acatctttac acaaattaat catttctttg aaacaggcga
     7141 aatccttccc gacctaccat ttgagaagat gttagatgtt gccgaggatc atctcactcg
     7201 actagttaat ttaaaaggtg aaactattgc cgttcgtgaa tttcgagggt tagcacctca
     7261 ttatctacgt ggaaaatcag gcgcagcaaa aattagagga gctgtttcac gtgcagaaac
     7321 actagcagaa gttcaagaac tttttgcagg tttacgctaa atcaaaaaca gtcctttgac
     7381 tgttttttcc tatctctgta caaatttagc ataaatcatt gtgtaatgaa aactcaaaag
     7441 tataacaaat tattttagta taacagaatt aaggagaatt atgttgattg ttttagctgg
     7501 aaccattgga gcaggtaaaa gctcccttgc agctgctcta ggacaacacc tagggacaga
     7561 tgttttctat gaggcggttg ataataaccc agttttagat ctttactatc aagatcccaa
     7621 aaaatatgct tttttactac aaatattctt tttaaataaa cgattccaat ctataaaaga
     7681 agcctataag gctaacaaca atgttttgga tcgttctatt tttgaagatg aactattcct
     7741 cactctaaat tttaagaatg gaaatgtgac taaaacagag ctagatatct ataaagaact
     7801 tcttgcaaat atgttggaag aattagaagg tatgcctaag aagagacctg atttgctagt
     7861 ctacattgat gtttcatttg ataaaatgtt agaacgtatt gacaaacgag gacgcagctt
     7921 tgagcaagta gattctaacc cagaattata cgactactac aaacaagtcc actctgaata
     7981 tccagaatgg tatgaaaatt acgacgtttc acctaaaatt cggatcgatg gtaacaaact
     8041 tgatttcgtc aaaaatccag aggatcttca acacgtcctt gacactattg attccgaatt
     8101 gcaaaaactt gatttactat aattaggtga tatctatgat tataagacct gtcctaccct
     8161 cagatgctaa agaattactt gctatctatg ctccctacgt tactgacact acaataacgt
     8221 ttgaatacga tattcctagt atatcggaat tcactaatcg tatcaaaaat atcagcaaat
     8281 ctttcccata tttagttgct gaagaaaatg ggaaaatatt aggttatgca tatgcttcaa
     8341 cttactacgc tagagcttcc tatgactgga cttgcgagct ttctatttac cttgataaag
     8401 atgtacgtag caaaaaaatt ggtagccaac tatatgatac attggagaaa gaattagtta
     8461 accaagggta tgttaatcta ctcgcttgta tcagtttacc aaatgatata agcattgcat
     8521 tccacaaaaa aagaggattt aatcaagtcg cacatttccc aaaaatcggg tttaaatttg
     8581 aacaatggca tgacattgta tggttacaaa agcgattgaa atcttaaggt aatctttaga
     8641 tttattttaa gttagcaaaa gattaggata ttataatata tttatcctaa aatttttcaa
     8701 cgactttcag tcgtacctat atctacaagt gcaattgtta gatatagatc cttttacttt
     8761 tcataaatct ata
//
LOCUS       DATRME010000078         8592 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRME010000078 DATRME010000000
VERSION     DATRME010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            84..731
                     /locus_tag="VBC23_001742"
     CDS             84..731
                     /locus_tag="VBC23_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6843671.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            complement(771..1460)
                     /locus_tag="VBC23_001743"
     CDS             complement(771..1460)
                     /locus_tag="VBC23_001743"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6843672.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(1470..1739)
                     /locus_tag="VBC23_001744"
     CDS             complement(1470..1739)
                     /locus_tag="VBC23_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843673.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(1739..2293)
                     /locus_tag="VBC23_001745"
     CDS             complement(1739..2293)
                     /locus_tag="VBC23_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6843674.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(2314..3693)
                     /gene="glmU"
                     /locus_tag="VBC23_001746"
     CDS             complement(2314..3693)
                     /gene="glmU"
                     /locus_tag="VBC23_001746"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6843675.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(3937..4350)
                     /locus_tag="VBC23_001747"
     CDS             complement(3937..4350)
                     /locus_tag="VBC23_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6843676.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            complement(4363..4740)
                     /locus_tag="VBC23_001748"
                     /pseudo
     CDS             complement(4363..4740)
                     /locus_tag="VBC23_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(4829..5785)
                     /locus_tag="VBC23_001749"
     CDS             complement(4829..5785)
                     /locus_tag="VBC23_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6843677.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(5782..6234)
                     /locus_tag="VBC23_001750"
     CDS             complement(5782..6234)
                     /locus_tag="VBC23_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO6843678.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            complement(6231..6476)
                     /locus_tag="VBC23_001751"
                     /pseudo
     CDS             complement(6231..6476)
                     /locus_tag="VBC23_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(6473..7171)
                     /locus_tag="VBC23_001752"
     CDS             complement(6473..7171)
                     /locus_tag="VBC23_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6843679.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(7164..7400)
                     /locus_tag="VBC23_001753"
     CDS             complement(7164..7400)
                     /locus_tag="VBC23_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6843680.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(7490..7738)
                     /locus_tag="VBC23_001754"
     CDS             complement(7490..7738)
                     /locus_tag="VBC23_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843681.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            complement(7936..8436)
                     /locus_tag="VBC23_001755"
     CDS             complement(7936..8436)
                     /locus_tag="VBC23_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843682.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
BASE COUNT         2698 a         1828 c         1172 g         2894 t
ORIGIN      
        1 gtatttgtca aatacttttt cagtattttt tgatataatg gtcttagaaa aatagatttt
       61 aagaagtata agaaagactg aatatgactc caaataaaga agattaccta aaatgcattc
      121 atgaattagg tgaaaaaaac aacaaaattt ccaataagaa aatcgctgaa atgatgcaag
      181 tctcggctcc tgccgtgtca gaaatgatta aaaaaatgat aagtgaacaa ctgattgtta
      241 aagataaaga tttaggatat tatcttacca aacaaggttt attagttgtt tctgaccttt
      301 accgtaaaca tcgtctagta gaggtatttt tagttaacca tttgcactat actgctgatg
      361 atattcatga agaagcggaa gttttagaac atactgtatc aacaactttt gttgatcaat
      421 tagaaaagtt gttagacttt ccacaatttt gtccacacgg tgggactatt cctaaaaagg
      481 gagagttttt agtagaaatt aaccaaatga cattggacca aattagtcag cttggcacat
      541 atgttatcag tcgtgttcat gatgattttc agctactgaa atacctagaa caacaccgtt
      601 tgcatatcaa cgataccatt gaattgacac aaattgaccc ttatgcaaaa acttatcaca
      661 tcacctataa cgatgaaaat ctaacaatcc ctgagcgtat cgcaagtcaa atatatgtat
      721 ctgctacata aataaaaaag tccgtgtatt cattagatac acgggctttt ttataatgct
      781 ttcaaaaatg ccattaatac ttgggcagag cgtttaccag cttcaatgat aaattcatca
      841 aatgtgatat tggcatcatg agctgcagta tcactcatag cacgtaccac cacaaagggc
      901 tttcctgtag cttgagcagc ttgggcaatt gctgcacctt ccatctcgac agctaaaacc
      961 tgtggaaaat gccccttaat aacatcaatc ttttcctggc cagcaataaa actatcccca
     1021 gttgcaatta aaccaacttt actagtcatt tcttctttag ataaaactgc ttcaaaagtg
     1081 gaaacaaaag tcttatccga atgaaaataa agtggctgca ttgacatttg tccatagtca
     1141 taaccaaagg cggttaaatc tacatcgtga taaactaatg tatctgcgac aacgacgtct
     1201 cctacattca atcctgttgc tactgcacca gctgatcctg tattaataat agcatcaact
     1261 ttaaagcttt ctactaaaat agcaacactc atcgctgaca tgactttccc aacaccagac
     1321 tgtactaaaa caagctcatg ttcaccataa cgtccactgt aatatacatt tgataatact
     1381 gtctcttgag atttatcctc aagattttcc acaaggagtt ttaactcttc ctccatggct
     1441 gcaataattc caattttcat gatatttcct tataatttaa aaattgcata aaacaagaga
     1501 gccaacaaaa taagcaaaat gaataaaact aaattcaact tacgttgaaa ttcatttcgt
     1561 tttgcattct caatccggcg actcttgtag atatgtttac cgttctcgtc agttagttgc
     1621 tccatgccat cttctgtaga aataatttta gtttcttgat ctaagattgt ttgacctgaa
     1681 actttttcac ctcgatttga acgttcaatc atatcatctg ttaacaatgg ttttcccatt
     1741 aagaatgacc tcccattttt aaaccgtaat attgtaaagc aatgatcgtt ttcgcatctt
     1801 gaatcatgcc tttttctacc atttgcatac aatcctcata acttaactct agaagctcaa
     1861 ggacctcatc atcatcttgt ggcctaggat tttcaacttt ttgtaaatca gtagctagat
     1921 agaggacgat tttctcatta caaaaaccaa tagcagtgta gaaactataa agaatttcaa
     1981 gattccctgt gtagccagtc tcttcttcta attcccgaag ggcagcttct tccttagaac
     2041 ctgactcacc cgtctcaagt ttccctgctg gaatttcgta agaaatacct tcaatagcct
     2101 tacgatactg tttcactaaa acaattttat gctcaggagt taccgccaaa gtagcaacag
     2161 ctcctccatg aaaaacaagt tcacgtttgg attgtcccaa tccatttggc aactctacat
     2221 catcaacagc tactttgata atttgtccat caaaaacagt ttgacgatta attgtttttt
     2281 cttcaaaatc cataagaacc tcccaaataa gtgttacttt tgtgaaggat gatgtggttt
     2341 tttattggca tatccttctt tattgacttg acgtcctctt ccaatagcta tgctatcaat
     2401 tggaacatta tctgtgatgg tagatccagc tgctgttaaa gcattatcac caatttcaag
     2461 tggtgcaatc agagtagaat tacttccgat aaagacatta cttccgattt ctgttttaaa
     2521 tttattttgt ccatcataat tgacagtaat cgtccctgcg ccaaagttaa catcacaacc
     2581 tacctctgca ttgccgatat aagttaaatg acctgccttc gtattctcac ctatttgaga
     2641 accctttact tcaacgaaat taccaatatg aacacctttt gcaagacttg ttcctggtct
     2701 aatatgcgca taagggccta cggttactcc atcagaaata atggattctt caaccatcga
     2761 attagtgatg gtaacatcat ttccgacttg agcatctact aaataactac cattggttaa
     2821 taaagtacct ttaccaatct tcgtctgtcc ttttaaagtc acattaggct caattacaga
     2881 ttcttctcca atctcaacat caatatcaat ataagcggaa tcaggattaa ccactgtgac
     2941 cccattaacc atatgttgac gtgcaattcg gtgacgcatc actttttcag cagttgcaag
     3001 tgctacacga tcattaactc ctagactttc gtcaaagtca cgaagtttat aggcacctac
     3061 ctttttaccc gcttctttga aaattccaat aacatcagtt agataatact ctccttgagc
     3121 gttatttgta ttaatgtctt taagtgcttc aaatagactt tgattatcaa aaacataagt
     3181 tccagtatta atctctttaa cctgctgctc aaagtcatta gcatcctttt gttctacaat
     3241 tttagtaact tcatcatctg aattacggat aatacgacca tatccaaatg gatttgcagc
     3301 atcggctgtt aagattgttg caacgttttt atgattgaca tgaaaatcaa ttaagttttt
     3361 taaactttct ccagtaatca ggggtgtatc acctgcaata actagtgtat gtccttttga
     3421 tgtcgctaat tcttcttccg ccatcattac agcatggcca gttcctagct gttctgtttg
     3481 cattacaaac tctgatttgt cacctaaaac atcacgtaca agctccgcct tatgtccaat
     3541 aacagtaaca attttacttg gctcaatagc ttgcacacta cggaaaacat gctcaagcat
     3601 ggtaatacca gatactttat gcataacttt tggtaaatca gatttcatgc gagttccctt
     3661 acccgcagct aaaataatgg cataatttga catattaatt cctctatctc tgaatataat
     3721 tcaacctatt atatcataat gccttcaact tatcactaat acccctttaa atccctatta
     3781 caatagactt tagctaaaat agcttacttc aattaggaat aaaatagcta ccttttggta
     3841 gctattcaat acatgaaatc cgttaatttt ttcttctctg tttacaatca gtatttttat
     3901 aaatctatca ttgcaatgct cagatggtaa aaacgattag tttaactctg ttgaaatttg
     3961 ccacatcaca ccaaattggt ctactaagtt tgcgtaagca ggattccaga aagtttcttg
     4021 taactctaac tgaacttctt gtgcactctt ctttaatttt tcataaattt cacgcgcctc
     4081 ctctacgcta cccacaataa tagcggctgt aacattttta ccagcttgat acacgaaatc
     4141 tggattttca tccgagattt gaagacgaat gccatcaaaa ataagctgag cattaatgat
     4201 taaatccttg cggtcttcaa gacagttggg gtccatctct tcccatgtca tcatattcac
     4261 taaatccgct tgaaagacat ctttataaaa atcaaccgct tgtcgcccat ttccatttgt
     4321 tacgatgtat gtttctagtg ctttaaccat aagttctcct ttttatttta cttcacggat
     4381 tgcaaagtaa ttattgtcat tatcggaaaa attaaaaact ctcatactcc ccatatcaac
     4441 aataggattt gtcataacct catttgctgt taattcttga tatgtactgt caatatcagt
     4501 agtttcaaat aaaatagagg gagttgctaa atctagctca ggactcattt gagctataat
     4561 tgctttgtca tgtaagacga agctaacttc actatccaac ttcggtgcaa ctatataggt
     4621 tacataatct ccttgtgtct atttttcaac acgctcaaaa cccaccttgt ttttccaaaa
     4681 atctgctgat gcttcaacat tgctaacgta gagcattacc tgtccaattg atgaaatcat
     4741 tttattatct cactttcggt tttattacta attagtataa catacagtat ttaaaataag
     4801 taaaaaatga ctcaaaagag tcatttactt atttcgttac catatctaga actttcatca
     4861 ccgttctact atgttctaaa gcttgagcag cacgcttaaa atctaaattg ctaataatac
     4921 cttcaaaaat aacaaattca tcgtgcatgc ggtgcctatc accatttaat tgataaacat
     4981 gactttcttg tccattcata gttaatgaaa ttttaggcat agtattagtt ggccctaaaa
     5041 tcgctataga ccccttatca ccttgaatag tagattttac ttctgcacta caatcttttg
     5101 ctccaataca gactgtttta aagtgcccat agtctaggac tagaactcct gaagtatcaa
     5161 taccacgctc tatatttggt aaatactgtg ctgttatagg ttctccaaaa agtccaatta
     5221 ctaaatgtaa attataaata ttaaggtctc tcaacgcacc tccacccatt tcaggattga
     5281 aagctggtgc tatttcccca cgtttaaagg catcataacg cgatgaatat tgggaataat
     5341 tacattctac aattttaata tctccaaggt tgcttaaatg ttcttttaca aggtcaaaat
     5401 taggtaaata ttgatttgta attgcttcga gtaatattag ttgcctagta ttggcaatag
     5461 atacaagttc ttctaattga cttgcttcta atgtaaatgg cttctcacaa atgacatgtt
     5521 ttcctgccaa gagtgcctcc ttagcatagt caaaatgcaa gtgattcggc agtccaatat
     5581 agactgtgtc aatctcctca ttgtccaaga cagccttata ttcactcgta gctagagaca
     5641 tgttatattc cttagctaga tctttagctg tctccaaact tctaactgtt gataagatag
     5701 caactagatc aataccttca attttctgca agacaggcaa aacttcctta acaatcatac
     5761 ctgtccccaa aaccgctaat ttcattcgtt aatctccttt gggtaaacca cttggaattc
     5821 ctctaaaact atcctagagt cctcactaaa cattaaacca aactcctcaa aataaggttt
     5881 aaagaaatca atgtgagctt ttctccacca ttctaaagtt ttatctcctt ctccttcttt
     5941 aaaagcatgg tctgcagaga catctttaaa agggacaata tcaactttag ttatttcaat
     6001 aatacagata gcttcatcat tgctatccaa aataatatca tatgaaccaa cttgtggtaa
     6061 cccttcctca tctaattcat ataaatcata ggcagaggcc gttgcagttt ttgtcccatc
     6121 tacaactaac tttgccagaa gatctggttc tgctccaaat tgccatgcgt ctatctcatc
     6181 accgattgaa ggattcaaag atttaaactt attccataat tcttgtgcct tcatattacc
     6241 tccgaagcaa ttcccacaac aaacttaact gcgttactca tttttctttg tcttttaaat
     6301 ccttctttaa catctaaaat ataaacagtt tgaccaatcc aaacacgtaa ataaacagat
     6361 tttattaaaa tatcacctga tattccttta acttcccgct cagtaccatc agatgcttca
     6421 aattccgtac gtaataacca agtattacca caaccaaatt caatctattt tctcatttca
     6481 aactccagcc accatcaatt ttgacaattt caccttgcat tgatctcgct tttcctgaag
     6541 caagaaatcc agttaattca gcaacttcac ttggtttagt ccaacgacca attggcgtct
     6601 cactagctac ccattctgct aatccaccag gttcaaaatc actagcagtc atagcagttt
     6661 gtacagcacc tggcgctata ccaaaaattt gaatacaatc ttttgcgtaa tccaaagcca
     6721 attgacgtgt gaatcccgca agggcatgtt tagaactagt atatgcagct ccaccgccac
     6781 ccgctataaa acttgctatt gagcacatat tgataataat acctgacttc ttttccacca
     6841 tacgtctaag ataatgacgt gttagcctaa cagtcacgaa aaaattaata tcaaataggt
     6901 gttccagttc ttcatcactt acttctagaa gtggcttgta ggcatctaga atacctgcag
     6961 tattacacaa aatatcaaca gtcggtacca ttgtgaatag tggggataaa tcagaggata
     7021 aatccaactt tataaagtta aaattaccat ttaagttcgg tttatccgac ttatccacac
     7081 catatacttg atacccttgt ttcaaaaaat attgagcctg tgcataacca attccactag
     7141 cacagcccgt cactaaaaca actttagtca ttgacctcga tccaatcctc tgctaaaaca
     7201 tcacatggtg ttggtgccca catggaaaaa ccttcacctt caccagtaac attaataaga
     7261 aaataaggtg tagcctgtag tactttccca ttaacttcaa gggtatcaaa taactgaaca
     7321 taattttcag cgtcccccca acctgtacgt acatattttt tctttgcttt taaacctggt
     7381 aaaatttctt caaatgtcat attgttctcc tttattgtct ttaaccattg atatatcaag
     7441 gtttattgct tatttaaaat cataatttaa agtttcttcg ccttttagtt catctaaagg
     7501 ctactattgt tttcatagga taaaatagtt tctggtcatc cattattagt aaataatatt
     7561 tcgtcttttt cttgttggtc aagaacatta tttttgttct tactccttca attcccacaa
     7621 taaagttatc tgcttgctcc gtcaacaaaa caggtccatt ttccactaat tcataagaaa
     7681 tacttagttg atttagcaca gctataactt tttcaaaatc attcgtaatt ttctccattc
     7741 ttccatagag ttagcataac tatttgtaga ctagagaacc taaaattgat acactgtttt
     7801 tcactcaaaa aattttacct tcataatctt cattatacct ataacaattt tcttttacaa
     7861 atttgttccc cttaaaggaa attgtttttc aaaataggat gtttatacta ttgtattatc
     7921 ggatgcttta tctcgctagt gcaaaatctt ctcataacac atttcttcct tttcaaaata
     7981 agtttttgat ttacccttaa aagaaaaacc aaacctatcg taaaatttcc ttgcgcgtgt
     8041 attttctgta aatacccaaa gtatcacttt attataacct gcttgttgta tctctgatag
     8101 tacatgattc atcatttgag acccataacc ttttccccaa ccttctttaa gactatgtat
     8161 acaaatgagt tctgcataat caagcatacc atcttctcta cttttatccc accaagccat
     8221 acaatgagga ttgctatcta cttccaatat atacccctta ccaacctcct tgtgtagtag
     8281 ctgttgatac attgttatgg caggttctat ttccgtagtt ttttgaatga tatcctcagg
     8341 tagtatctta cccaaagcag ctttccagct ctccgtttga atataagcta aattgacttc
     8401 atctcctaat cttgctctcc tgattatgca agccataact aaaatccttt caaaaattat
     8461 tattaactgc ctgataacta ctccaattat accattttcg tgatattaat ttttattttg
     8521 aacagttctg gttctcattt atcattatat ttctaaaatt ttagatggtt ttaaacatta
     8581 acctcagatt ag
//
LOCUS       DATRME010000079         8455 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRME010000079 DATRME010000000
VERSION     DATRME010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..739)
                     /locus_tag="VBC23_001756"
     CDS             complement(<1..739)
                     /locus_tag="VBC23_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6843683.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFY"
     gene            complement(751..1449)
                     /locus_tag="VBC23_001757"
     CDS             complement(751..1449)
                     /locus_tag="VBC23_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843684.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(1450..1818)
                     /locus_tag="VBC23_001758"
     CDS             complement(1450..1818)
                     /locus_tag="VBC23_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6843685.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            1963..5067
                     /locus_tag="VBC23_001759"
     CDS             1963..5067
                     /locus_tag="VBC23_001759"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6843686.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKNRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            5148..6170
                     /gene="pfkA"
                     /locus_tag="VBC23_001760"
     CDS             5148..6170
                     /gene="pfkA"
                     /locus_tag="VBC23_001760"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6843687.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            6219..7721
                     /gene="pyk"
                     /locus_tag="VBC23_001761"
     CDS             6219..7721
                     /gene="pyk"
                     /locus_tag="VBC23_001761"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6843688.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            7892..8449
                     /gene="lepB"
                     /locus_tag="VBC23_001762"
     CDS             7892..8449
                     /gene="lepB"
                     /locus_tag="VBC23_001762"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6843689.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
BASE COUNT         2747 a         1316 c         1660 g         2732 t
ORIGIN      
        1 tatagaatat agcaatttca acaatgctaa caagtatatt gtagagcaat acaggttgag
       61 agaatgttgc atttgcatga gagttgaata aatcaatatg aaaaatacta tctattatta
      121 atgttaagaa agtaattaag agtgatattc caaaataaga aacaattccc atcaatacac
      181 gtttgtttgt aaataattgc cctacagcga tagagagata aatcagtaat gtccctgcaa
      241 cgtacgatag aaagaaatag gcgataaaaa gtggagcgtt cgaagatgaa ataattttat
      301 agatttctgg aaaagcgaca acgaactgac caatacctac taaaggtaat atgaccaata
      361 tgattccaat aatgacaata aaaatattaa aaatactcca aagaagcgaa gctagtagct
      421 ttgaacaaat aatctgattc gtagttactg ggagcgttaa ggttagatat ccttgacggc
      481 cgaagatatt gctataaaaa cgtttgataa taatagcaag ggtggataac agagatcctg
      541 aaataaccat cgctaacaat aaaacgagta ttatagtgaa tatttgggcg ctagtactat
      601 tagtatcagt agaaaaattg ccgccaagtg cctttaatac cagtcctaaa ataatagata
      661 ctaataaaac cgcagcgttg agtgttagat accacttacc aactgatttt aattcatatt
      721 ttaataattt tccaaacata tcaatctcct ttatttcttg aatctttcac gaaagagagc
      781 atcaatcgaa cagttgtgct cttctcttaa gtcatcagca ttgccttgta ggtcaatttc
      841 tccttcttta aggaagataa cttcatcaag gataggttca atgtcagaaa ttaagtgagt
      901 cgaaattaaa acagaagcat cattagagta gttgctaatg attgttttta aaatgtagtc
      961 acgcgctgca gggtctacac caccaatagg ttcatcaagg atgtagagcc tagctttacg
     1021 actcataaca agtattagtt gaactttctc cttgttccct tttgataggt tttttagacg
     1081 ttccttagga tttaggttaa gatcctcaaa tagttgataa gctacctttg aattaaaatc
     1141 gttatagaaa tcttcaaata atgtaacgac gtctttgatt ttcatatttt cttgtaaata
     1201 tgaagtatca gggagatatg aaataatttt tttggtttca acagacggtc tgtaaccatc
     1261 gataacaata tctcccttat ctggctgaag taatccattt atcattttga tgattgttgt
     1321 ttttccgcta ccatttggac ctaataaacc tataatcttt cctgttggaa tgcttacagt
     1381 cacatcatta acagcaatat gtttgtgata tttctttgta acatgatgaa gttgtaataa
     1441 ctgtgtcatt taattaatct cctttataaa atgatcgaga gcaaccgtaa tttcatctaa
     1501 tttatatcct atttttttca tgttgttgac aaaagttgct aactctgctt ttgcaacttg
     1561 ttgacgaatt ttaccaatta gtaagttgtc ttctgtaaca aagcgtccgg aagtacgttg
     1621 tgagaatacc attccttcac gttccaattc agtgaaggca cgttgcatgg tatttgggtt
     1681 aacaccagct tcttgagcta actctctcac ggtaggcaat tggtcaccac tttttatttc
     1741 ttgactaaca atttgcatct ttatatgttc agcaatttgt gaataaatag gtgatttttc
     1801 attgaattcc caagccatag ataactcctt tcataatctt gtactttttg tactataaaa
     1861 atagtacaaa aaaatatttg aaatgtcaag gaattatcat tgagacttta atcgtttatt
     1921 gtttcatagt ataatagtaa aaaatcgtaa aaaggggcta ctatgtttgc acaacttgat
     1981 acaagaaccg tttattcttt tatggatagc ttggttgatt taaagacata tgtctctaaa
     2041 tcgaaatcat taggatatca aactattggt atcttggacc acagtaatct ttatgcagca
     2101 tatcatttta ttcaagaggc gcaaaaggca aacctaagac caatagttgg atttagcttt
     2161 gatatagtgg ttgaaaatag atcaatagaa gtttactgta ttgcgataaa tacagttggt
     2221 tataagaatc tcttgaagtt gtcaacggct caaatgtctg agaagatgtc tctaaattta
     2281 ttgacagaac atcttgaagg ggtgcaattg attcttccct atcaagatgc tcttgatcag
     2341 ctcaatttac cctttgatta tgttattggt gttaatttga cctctccgca gattccatat
     2401 accaaaccta ttatagccat agatactgtt cgatattttc aaaaaaatga tattgagaca
     2461 ttacagatgc ttcatgctat aagagataat gtcctgttaa aagatgcaca atatgcgagc
     2521 aaaaatcaag aattaaaacc ttgtcaagaa atgactctag cattcaagaa ccgttttcct
     2581 gaagctttag ctaacctaga atctttggta gagaatgttg tttatcactt tgattctgac
     2641 tttaagttac ctatttttaa tagagaaatt cctgctggag aagaattaag aaccttaact
     2701 caaaataact taaaaagtaa aggtttatgg agcgatgctt atcaagagcg tctcgaaaaa
     2761 gaattaacaa tcattcacaa aatgggcttt gatgactatt tccttattgt gtgggattta
     2821 ttaagatttg gtcgtagtaa gggctactat atgggaatgg gacgtggttc agcagcggga
     2881 agtttagtct catatgcttt aaatattacc ggtatcgatc ctgtcaaaca taatttaatt
     2941 tttgaacgtt ttttaaatga agagcgttat tcgatgccag atattgatat tgatcttccg
     3001 gatatttatc gtggagaatt cttgcgctat gtacgtaatc gatatggttc aatgcattct
     3061 gcacagattg taaccttctc aacttttggt gcgaaacagg ctattcgaga tgtttttaaa
     3121 cgctttggtg catcagagta tgagttaaca aacataacta aaaaaattca tttcagagat
     3181 aatttgacca gtgtttataa ccgcaactta gctttcagac aaataataga tagtaaaatt
     3241 gaataccaaa aagcctatga tattgctaag cgtattgaag gaaatcctag acagacttct
     3301 attcatgccg ctggggtagt aatgagtgac gatttgttaa cagatcatat tcctttaaaa
     3361 aacggtgagg atatgatgat aacgcaatat gatgctagtt ctgttgaggc taatggtctt
     3421 ctaaaaatgg actttctagg tcttcgaaat ttgacatttg ttcagaaaat gaaggagaaa
     3481 gtcgataagg actacggtat ctctatacaa ttagaagcta ttgatttaga agataaagag
     3541 actctaaaac tatttgcggc tggtcaaaca aaggggattt ttcaatttga gcaaagtgga
     3601 gctattaatt tgttgcgacg tattaggcca gagtgttttg aagatgtggt agctactacc
     3661 agcttgaata ggcctggtgc aagtgattat actgagaatt ttataaaccg tcgttttggt
     3721 aaagaaaaaa tcgatttagt agatcctgtc atagctccaa ttttacaacc tacctatggc
     3781 ataatgctct atcaagaaca ggttatgcag attgcccaga cctacgcagg ttttacgcta
     3841 ggcaagtctg atttgctcag acgtgctatg tctaagaaaa attctaagga gatgcaaaag
     3901 atgtctcaat ccttcttaga gggtgcagta tctaagggac atcgtcaaga agatgctcga
     3961 cttatatttg aacgcatggc taaatttgca ggttatgggt ttaatcgcag ccatgccttt
     4021 gcttattcgg ctttagcttt tcaattagcc tatttcaaag cccattattc cgatgtcttt
     4081 tatgacatca tgataaatta ttcaaatagt gattatctaa tcgatgctat tgattttggt
     4141 tttgttattg aaaaaccttc aattaatact atttcataca gagaccgcat ctataaaaag
     4201 aaaatttatt taggtttaaa aaatattaag gctgttccta acgacttggc ttattggatt
     4261 tctaaaaatc aaccatttca gagtattgaa gattttctaa tgagactgcc acagcagttt
     4321 caaaaaagtg gttttatttc tccgctaatt gctattggag catttgacga atttgataac
     4381 aatagacgga aaatcacctc taatcttgat tctttattta catttgtaaa tgaactaggt
     4441 agtttatttg cagatacctc atatcattgg ttagaagtag aagatttctc taattctgag
     4501 aaatatgaaa tggaacaaga tatcttagga gtaggaatca gtccgcaccc tttagtgggg
     4561 atttcacaaa aagctagccg tccgtttata ccaattagtc aagtccaaga aaatagcgaa
     4621 gctactattt tagttcagct aaagcaggtc aaaatcatta ggacaaaaag ttctggacaa
     4681 caaatggctt tcttgactgt tatggatatt aattcaaaaa tggatattac tgtttttcct
     4741 gaaactttca atattgtaag agatgattta caagaaggga aatattatta cttgcatgga
     4801 aaaattcaga agcgagatga gcgattacag atggttttaa acggtgttca agaagcaacg
     4861 gaggaacgtt tttggatact tctaaaaaat catgataacg acaaaaaaat atcggaaata
     4921 ttgtccaaat ataaagggca tattcctgtc tatttgcatt atgaaacaac taaagaaact
     4981 atccaaagca aagtacattt ggttagaaaa gatagtggct tagcccttga tttatctgaa
     5041 tttgttgtga aaacggttta tcaataaaat tgcatataaa gtgcagaaat ttacaaatta
     5101 atatgctata attaaatcgt taaattaata aaaggagtac caattaaatg aaacgtattg
     5161 ctgttttaac tagtggtggt gacgcccctg gtatgaacgc tgctatccgt gcagttgttc
     5221 gtaaagcaat ttctgaaggt atggaagttt acggcatcaa ccaaggttac tatggtatgg
     5281 tgacagggga tattttccct ttggatgcta attctgttgg ggatactatc aaccgtggag
     5341 gaacgttttt acgttcagcc cgttatcctg aatttgctga acttgaaggt cagcttaaag
     5401 ggattgaaca gcttaaaaaa cacggtattg aaggtgtagt agttatcggt ggtgatggtt
     5461 cttatcatgg tgctatgcgt ctaactgagc acggtttccc agctgttggt ttgccgggta
     5521 caattgataa cgatatcgtt ggcactgact atactattgg ttttgacaca gcagttgcga
     5581 cagcagttga gaatcttgac cgtcttcgtg atacatcagc aagtcataac cgtacttttg
     5641 ttgttgaggt tatgggaaga aatgcaggag atatcgctct ttggtcaggt atcgctgcag
     5701 gtgcagatca aattattgtt cctgaagaag agttcaatat tgatgaagtt gtctcaaatg
     5761 ttagagctgg ctatgcagct ggtaaacatc accaaatcat cgttcttgca gaaggtgtta
     5821 tgagtggtga tgagtttgca aaaacaatga aagcagcagg agacgatagc gatcttcgtg
     5881 tgacgaattt aggacatctg ctccgtggtg gtagtccgac ggctcgtgat cgtgtcttag
     5941 catctcgtat gggagcgtac gctgttcaat tgttgaaaga aggtcgtggt ggtttagccg
     6001 ttggtgtcca caacgaagaa atggttgaaa gtccaatttt aggtttagca gaagaaggtg
     6061 ctttgttcag cttgactgat gaaggaaaaa tcgttgttaa taatccgcat gaagcggatc
     6121 ttcgcttggc agcacttaat cgtgaccttg ccaaccaaag tagtaaataa gaatttgtca
     6181 atttgacact ttaaataaaa aggagctcat aaattattat gaataaacgc gtaaaaatcg
     6241 ttgcaacact tggtcctgcg gtagaattcc gtggtggtaa gaagtttggt gagtctggat
     6301 actggggtga aagccttgac gtagaagctt cagcagaaaa aattgctcaa ttgattaaag
     6361 aaggtgctaa cgttttccgt ttcaacttct cacatggaga tcatgctgag caaggagctc
     6421 gtatggctac tgttcgtaaa gcagaagaga ttgcaggaca aaaagttggc ttcctccttg
     6481 atactaaagg acctgaaatt cgtacagaac tttttgaaga tggtgcagat ttccattcat
     6541 atacaacagg tacaaaatta cgtgttgcta ctaagcaagg tatcaaatca actccagaag
     6601 tgattgcatt gaatgttgct ggtggacttg acatctttga tgacgttgaa gttggtaagc
     6661 aaatccttgt tgatgatggt aaactaggtc ttactgtgtt tgcaaaagat aaagacactc
     6721 gtgaatttga agtagttgtt gagaatgatg gccttattgg taaacaaaaa ggtgtaaaca
     6781 tcccttatac taaaattcct ttcccagcac ttgcagaacg cgataatgct gatatccgtt
     6841 ttggacttga gcaaggactt aactttattg ctatctcatt tgtacgtact gctaaagatg
     6901 ttaatgaagt tcgtgctatt tgtgaagaaa ctggcaatgg acacgttaag ttgtttgcta
     6961 aaattgaaaa tcaacaaggt atcgataata ttgatgagat tatcgaagca gcagatggta
     7021 ttatgattgc tcgtggtgat atgggtatcg aagttccatt tgaaatggtt ccagtttacc
     7081 aaaaaatgat cattactaaa gttaatgcag ctggtaaagc agttattaca gcaacaaata
     7141 tgcttgaaac aatgactgat aaaccacgtg cgactcgttc agaagtatct gatgtcttca
     7201 atgctgttat tgatggtact gatgctacaa tgctttcagg tgagtcagct aatggtaaat
     7261 acccagttga gtcagttcgt acaatggcta ctattgataa aaatgctcaa acattactca
     7321 atgagtatgg tcgcttagac tcatctgcat tcccacgtaa taacaaaact gatgttattg
     7381 catctgcggt taaagatgca acacactcaa tggatatcaa acttgttgta acaattactg
     7441 aaacaggtaa tacagctcgt gccatttcta aattccgtcc agatgcagac attttggctg
     7501 ttacatttga tgaaaaagta caacgttcat tgatgattaa ctggggtgtt atccctgtcc
     7561 ttgcagacaa accagcatct acagatgata tgtttgaggt tgcagaacgt gtagcacttg
     7621 aagcaggact tgttgaatca ggcgataata tcgttatcgt tgcaggtgtt cctgtaggta
     7681 caggtggaac taacacaatg cgtgttcgta ctgttaaata atttttgaat tcaaacctat
     7741 tatttaagaa taccttaaat aatgggtttt ttatatgttt tgctttttat tattcgatta
     7801 ttgtgactag aataggatat gatattaata attaccctcc tttttttatc tatgaaaaca
     7861 tgatataatg gaaaaaagat taggagtagt tatggttaaa cgcgatttta ttagaaatat
     7921 tattttagca ttgattgctg tattgatttt gattctcttg agatattttg tttttgcaac
     7981 ctttaaagta cataaggatg ctacaaattc ttattttagc aacggagatg ttgttgttgt
     8041 taatcgtaat aggacgccta agtacaaaga ctttattgtc tataaggttg gaaagatttt
     8101 ctatattagt cgtgttattg gtgagccaaa tcaaaaagtg agagtaatgg atgatatttt
     8161 atatttgaat gatgttttca aggatgagcc ttacattgaa aaaatgaaaa atgcttattc
     8221 tgaaaagaaa gacggtcaaa tgccctttac ttccgacttc tcagttgaaa cattaacacg
     8281 aaataaagaa agcagagttc ctaagggttc ttatcttgtg ttaaatgata atcgacaaaa
     8341 taaaaatgat agtcgaaaat ttggattaat caaagagaaa gatattcgtg gtgttattac
     8401 ctttaaagta taccctctaa gtgaatttgg ttttacagca tcagagtagt aaaag
//
LOCUS       DATRME010000080         8449 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRME010000080 DATRME010000000
VERSION     DATRME010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..273)
                     /locus_tag="VBC23_001763"
     CDS             complement(<1..273)
                     /locus_tag="VBC23_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6843690.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLI"
     gene            complement(409..957)
                     /locus_tag="VBC23_001764"
     CDS             complement(409..957)
                     /locus_tag="VBC23_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6843691.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(1130..2482)
                     /locus_tag="VBC23_001765"
     CDS             complement(1130..2482)
                     /locus_tag="VBC23_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6843692.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            2688..4037
                     /gene="trkA"
                     /locus_tag="VBC23_001766"
     CDS             2688..4037
                     /gene="trkA"
                     /locus_tag="VBC23_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6843693.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            4042..5481
                     /locus_tag="VBC23_001767"
     CDS             4042..5481
                     /locus_tag="VBC23_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6843694.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(5505..5756)
                     /gene="yidD"
                     /locus_tag="VBC23_001768"
     CDS             complement(5505..5756)
                     /gene="yidD"
                     /locus_tag="VBC23_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6843695.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(5756..6478)
                     /locus_tag="VBC23_001769"
     CDS             complement(5756..6478)
                     /locus_tag="VBC23_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6843696.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(6468..7052)
                     /locus_tag="VBC23_001770"
     CDS             complement(6468..7052)
                     /locus_tag="VBC23_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6843697.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGHPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(7049..7756)
                     /locus_tag="VBC23_001771"
     CDS             complement(7049..7756)
                     /locus_tag="VBC23_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6843698.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(7756..>8449)
                     /gene="xerD"
                     /locus_tag="VBC23_001772"
     CDS             complement(7756..>8449)
                     /gene="xerD"
                     /locus_tag="VBC23_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6843699.1"
                     /translation="NSRKSYHYDLKQFYKIIGGHVNSEKLALYQQSLSEFKLTARKRK
                     LSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLSNFYQDTDYPSGRLIAL
                     LILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPEDLLPFLLESLEEDGDL
                     VFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILKQKENGKTMTELSRLLG
                     LKTPITLERYYR"
BASE COUNT         2735 a         1601 c         1337 g         2776 t
ORIGIN      
        1 aattaataat aatgattttt tatcaaattg ataaccatgt tcttgcactt cccaattaga
       61 gatagcttgg tataaactgt ctttaggact atcatagcca aaaaccattt ggtgtgaaaa
      121 atcttcaata gcctccgaca tttctggaaa gataggaaaa tccatatccg caatccaaag
      181 gggaaggatt tcttgatctt tttgaaccgc cttccatttt atggcattac ttgaaaaacg
      241 ttctggtaaa cttgtgaaat catatgtaat cataattact cctgactatt ctacagctaa
      301 aaatatccct tgtcgccttg gtgactattt taccatttgt ttttgatact atatactaat
      361 taacattcta aagataaaag tgaccatttt aaacgatcac tttctttatt attttaattt
      421 atcattttca taagtatggc ttaattctaa attaggaaag ctttgctgcc taagggcttc
      481 ataaacaatc atacaaacag tgtttgaaac attcaagcta cgcacatgct catcgttcat
      541 gggaattcgt aaggcctttt cggcatgctc gcgcataaaa gtttctggca atcctttatc
      601 ttcacgacca aacataaaat aatggtcttg atcatcattg taattttcat ctgagtaaac
      661 cttgtcagca aatttgctaa ttaggtgaac tttccctcga catgacaaca taaattcttc
      721 caaaccatca tagaaactaa catctagctt atcccagtaa tccagtcccg ctcgtttcat
      781 ttttttatca tcaataggaa atcccatcgg tcgtatgatg tgcaaaggag catttgttgc
      841 cgcacaagtt ctagcaatat taccagtatt tgctggaatc tgtggttcaa ataaaacaat
      901 atgatttcgt ccgctatcgg aacgatgatt tttctgcgtt agtgtttcaa tattcatctc
      961 atcatcactt tctagaactc attctgactc gcaaaaaaac cacactgctc ggagtcaatc
     1021 tcagcaaaca atgtggttta tggtggtttg ttaacttaaa tcataacagg tttgatttaa
     1081 actaacaaat cgttgacact attatagcac ttcagtctac aaagtcaacc tattcatgag
     1141 tttttgggtt atttttttga tagatataat gacaaatcaa acagaataat aagcaaaaac
     1201 attggaaaat taaaaccgta aagaaattac ttaacaaact cccgataagg aggatcaaac
     1261 cgcctaaaat tgctagaatc ggtgaaacta agccggtaaa tttattttta atatcttttt
     1321 tgagataaag gctgagtact aaaacataaa gaatgattaa actggtatta ttaaacacca
     1381 cagcaatttc actaatatca ctacccggca ataagttaaa ttttgtaacc ataaaatgaa
     1441 caaaaagcca aaaaatagct acagccgtca ccgtcaaact agctggcaat gacatttgat
     1501 acttcaaatt tatgttagcc atcctctcac ttttgatcca tccgcgttcc gcaaaagctt
     1561 gaggtagacg catcgtccca agtaaaagtc cattggcaac acctaataca gatagaataa
     1621 caatgaaaga taataaacgc ccaacactag gaccgaaaat aatattagct gcataattaa
     1681 tagcatcatt accggttgtc ataataaagc tagcacctaa aatttgtgtc aaaccataga
     1741 aaaatgccaa atatgataat aagataagcg ctggaccaat gacaaaggca agtggtaaat
     1801 tcttcttggg attcttgact tctggtgcaa tattaacaaa aattgtccaa ccatcatatg
     1861 caaaatacaa tggtaccaga cctgacaccc aactccatcc aacatttgac ggttgaatag
     1921 ctgttaaatg ttgtggaagt tgtggagctt tatcagacca aaaagcacca attaaagcaa
     1981 ttagaactaa aggaatcatt tttacaaagg tcgttaaaga ttggaatccc cctgcaatac
     2041 gcttagcaaa gatattcatg agactaagcg ctaaaatata aacagcagct aaaataatct
     2101 gtgattccaa acttgatgat tcacctaaag tataaaatgc agctacccat gaaacgattg
     2161 ctgttaaagt tggaagatac aaaaaagacg caaacaagcc taaagttgct gctaaagctg
     2221 ggctaacata tttttcgtag taagaaaaaa taccaccact ttcactcgtt ctaatagcta
     2281 gctcagaaat tgacaaactt ccaaaaacaa tcgaaaaaga acctagtact aaaattacca
     2341 tgcctaaaaa gacatctcca ccagtaaatt taaggatgtc atcaacttta aaataaatgc
     2401 ctgaaccaat cactacacct acaatcatag caattgtagt cattaatcca taggtctgtt
     2461 taatagaatt atgattctcc ataatatctc catttctact gtacgcttaa aggttaatta
     2521 taacctaatt tagggcttgt cacaagttta tctgtgttat ttttcaccag attctgataa
     2581 gagaatcata ttactcttat cagaaagatg aaaacgtttt ttaaccataa ttctcgtgca
     2641 tttttactaa aaaaaaggta tcatagagtt tattattgga ggtaaaaatg agaataattg
     2701 ttgtcggcgg aggtaaagtc ggaaccgcac tttgtcgctc acttgttgct gaaaagcacg
     2761 atgttgtttt aattgagaaa aaagaaaacg tccttaaacg tgtcactaaa cagcatgata
     2821 ttatgggaat tgttgggaat ggagcaaact ataaaatttt agaacaagcc gaagtcaaaa
     2881 attgtgatat ttttatcgct atcactgatc gagatgaagt taatatgatt tcagcagttc
     2941 ttgccaaaaa aatgggggca aaagagactg ttgttcgcat gcgtaatcct gagtattcta
     3001 atccttactt caaagataaa aactttcttg gcttctcttc agttgtcaac cctgaattgt
     3061 tagcagcaca atatatcgct aataccatag aatttccaaa tgcaacttca gttgaacact
     3121 tcgccaacgg gcgtgtgatg ttaatggaat ttaaaatttt agaaggaaat aaactctgtc
     3181 atacttcaat gagtcaaatt cgtaaaaaat ttggtaatat tgttatttgc gcaattgaac
     3241 gcgatggcaa attgattatt cctgatggcg atgcaactat ccaagtcaaa gataaaatct
     3301 ttgtaactgg aaatcgtatt gaaatgatac ttttccataa ttacgttaaa aataaagttg
     3361 tcaaaaactt gatggttatc ggtgcaggac gcatcgccta ctacctactc aatatcctta
     3421 aaaataccaa tacacacgtc aaactagttg aactcaacca agagcaagct gaatatttta
     3481 gtcaagaatt tcccaatgtt cctgtcgttc atggggatgg tactgctaaa aatattttgt
     3541 tagaagaaag cgttacaaat tttgacgcag ttgctacttt gactggtgtt gatgaagaaa
     3601 atatcattac ctcaatgttc ttagaaagta tcggtatccc taaaaacatt accaaagtta
     3661 atcgaactag cctactagaa atcattgacg ataaacaact atcaagcatt atcacaccca
     3721 agcgtattgc cgtagaccat gtgatgcact ttgtacgcgg gcgtgtaaat gctcaggatt
     3781 ccaacctaga agctatgcat catattgcta acgatcgcat tgaaacctta caatttgaaa
     3841 taaaagaaac tagcaaatta gcaaatcgta gccttgcatc tttaaaatta aagcaaaata
     3901 ttttaattgc agcgattatt cgcaataata aaacgatttt cccaactggt gaggatgttt
     3961 taacagtggg tgatcgtatc gttgttatca ccttattaaa aaatattact agaactagtg
     4021 atatgttagc gaggtaggaa tatgaataaa agtatgattc gtttccttct ctcaaaactt
     4081 ttgctaattg aagctgctct tctagcgatt cctctaactg ttggacttat ttaccgtgaa
     4141 ccgcaaagtg tcatgatgag tatagtcatt accatgatta ttttaatcat ccttggatta
     4201 ctgggttctc tctttaaacc taaaaattat catatctaca ctaaagaagg catgttaatt
     4261 gttgcactct gctggatact ttggtccttc tttggcgccc ttccatttgt tatttcaggg
     4321 caaatcccta atattatcga tgctttcttt gaagtatctt ctggttttac gacaacagga
     4381 gcaactattc ttgatgacgt cagtgtctta tcacccgcgc tcttattttg gcgtagcttt
     4441 acccatctta tcggtggaat gggggtttta gtatttgccc ttgccatcat ggaaaattct
     4501 aaaaatagcc atttagaggt tatgagagct gaagttcctg gacctgtatt tggtaaagtt
     4561 gtctcgaaac ttaaaaaaac tgcacaaatt ttgtatttgt tatatcttct tatgtttgca
     4621 gtatttgcag taatattata ttttgcaggt atgcctttct ttgatagtat tatcatagcg
     4681 atgggaactg caggtacagg tggttttgca gtttacaatg atagcattgc tcattataat
     4741 agtcctctga taactaactt agtttctatt ggtatgctta tcttcggggt taactttaat
     4801 ctttactatc ttcttttact ccgaaaaatt aaagcattct ttggtgatga ggaactaaaa
     4861 acctatctcc gcatcgtggc aatagcaact ttcatgattg ctctaaacgt catcggtatg
     4921 tatgataatt ttcgacaagg cctcgaacat attttctttg aagtatcagc tattattacg
     4981 acaactggtt ttggcgtgac tgatatcact cgttggcctt tgttttcaca ggttattttg
     5041 ctattcttga tgtttatcgg tggttctgct ggctctacag ctgggggctt taaagtcatg
     5101 cgtagcttaa tcctagcaaa gattgcacgt aaccaagtat tgtctacact ctatcctaat
     5161 agagtcatgt cactacacat taataaaagc gttctagata aaaatacaca acatggtgtt
     5221 ttaaaatatc taactatcta tttagctatc ttcatggcac tcgttcttgt tttaacgctt
     5281 gatactaatg attttttggt tgttatcagt gctgcagcct catgttttaa caacataggc
     5341 ccactcttag gtagcaatga aactttctca ttctttagtc cattctcaaa actccttctt
     5401 tcatttgcta tgatagcagg acgtttagaa atataccctg ttttattgat gtttattcct
     5461 aaaacatggt caaaactata attacaaata ataaaacgga tatatcaatc tgatatatcc
     5521 gttttattac gtcttaagct aaaatgatca gggacaggat catttcctcc gtgggctaag
     5581 ggatgacatc gcaaaatacg tgcaatcccc atcaacacac cttttagacc atgtttttga
     5641 atagcttcta tcatatacgt agagcaagtt ggacgataac gacagctagc tgggaaagct
     5701 ggagaaatat ttttttggta aaagcgaact aagaaaatca aaaaagattt aagcattaag
     5761 atttattaat agccgcatta tgcaactgac taatttcttt tttatttaat cgtcgtgctt
     5821 ccccaggacg gagacccgtt aaatctaagg taccgaattg tgtacgagat agcttgtcca
     5881 ctaataaccc aacttgttca aacattttct taacttgatg attacgacct tcatgaatag
     5941 ttaattcaac tactgaacga tttttttcat gatccacttt tatgatagta tagcgagcag
     6001 gttttgtttt cttaccatcg ataacaacgc cacgcgttaa tggtcgaagg ttttctttag
     6061 tagctattcc tttaacacgt gctaaataaa ctttatcaat ttcattacga ggatgaatca
     6121 ttttatcagt aaaatcacca tcattagtca aaattaaaag tccagttgta tcccaatcca
     6181 agcgaccaac aggatatatc cgttctttga cttgtggcaa caagtcaata actgtcttac
     6241 gtcctttatc atctgagaca cttgaaataa caccacgtgg tttatttaag agataatata
     6301 ccttctcttc gttgtaaatt ggacttcctt caatttcaac cagatcacca gcctttactt
     6361 gggtcgccaa ttcattaacc acttgaccat taatcgtgac cattccttgc ttgattaatt
     6421 cctcagcttt tctacgactt gcaatccctg catgcgcaat atatttatta attctcattg
     6481 ttttccttat cttctgtatt aattgaatcc attgaaaata acgatacttc ttcgtcaact
     6541 aattcaattg aagaggcgtc aattaagtca tctaactgat taattcccat ataatctaag
     6601 aaataattag ttgtctcata aaggttagga tgtcccaaca cttctttctt tccagcctct
     6661 ttaattaaac caaaagctat cagtctcgtt atagctccac tggaattcac gccacgaata
     6721 tcatcaattt caatcctagt aataggttgt ttataagcaa taatagatag aacttccaaa
     6781 cttgcccgag atagactctg attaatcgga gccttcgcat aatctctcaa caaagtcata
     6841 taactatcct ttgttaccaa tttataagtt tgtgctgtct caagcaataa tagactagaa
     6901 tcgtcatctt cttcatatcg tttagccaac ttttctagtt gctgtattaa tgctgatggt
     6961 gtcaaagaca ataattctgc catttgacgc aaactcagtc catcttcacc tgcaacaaaa
     7021 agaagagctt ctattgagcc taaataagtc atttctcttc ctttcttaag ataacttgac
     7081 taaaattact gtcttgttct actgtgattt gatgtaattt tatcaactct aacattgcca
     7141 aaaataaagt tatcatttca gattttgttt ggcaatccgc aaacacttct tgtaatgtcg
     7201 ttttcttttt aagattaaaa tgtctttcaa taacgatcat catatcttca atacggtagt
     7261 cttctttctc aattaccgtt tgactatttg acaattcttt ttgtttttgg gacatcattt
     7321 gagaaaaagt caaaaaaaga tccatcaccg atttatcgtg tagaagaatt gcatcttcaa
     7381 agataacttc ttgttttggt tttgaaaaat atttggcgcg ttcctgatgc tgattagcta
     7441 actcttcact tagcacttta aaacgtctat attcctctaa ttggctcaac aattccattt
     7501 ctggatcatc ttcaatagga tttgactcca ctactttagg taataagtta cggcttttta
     7561 tcaacatcag ctgacttgcc ataagcatat actcaccagc tacttctaga cgcatcgcct
     7621 gtaatgttgc tatataagca aggtactgct caataacttc cacaatcggt acatcgtata
     7681 tatcaacctc atatttagat acgaggtgca agagtaagtc taggggcccc tcgaaatctt
     7741 ttaacttaat atccattatc tataatatct ctctaaagtg ataggagttt ttaagcctag
     7801 aaggcgactg agctccgtca tcgttttgcc attttctttt tgctttagaa taaattgttc
     7861 tcgcagaagt tgtgcagtta gttgttgctc attcttctca tttaagaaat ctgttaattg
     7921 attaaaaaac cattgtctag aataaggctt accgttatgc tcaaagacta agtcaccatc
     7981 ttcctctaaa gattccaaaa gaaaaggaag caagtcttct ggaagtttta aaatacgctt
     8041 catctgcgat ttttcaatac ttaagatatt aaaagttgta tcaaaatcag ccttcttcaa
     8101 attagcaatt tcagccggag ttaaacctaa agataaaatc aacaaagcaa tcaaacgacc
     8161 actcggataa tcagtatctt gatagaaatt tgataaatcc attatttgag actttgtctg
     8221 agccaaagtg attttttcag tctcttgtag tctgtaaaat tccttcagcg ttcctctatt
     8281 atataaaaag aaaagaaatt gattgactgc agataacttt cgcttacgag ctgttaactt
     8341 aaattcactt agagactgtt gatacaatgc cagtttttca gaatttacat gtccaccaat
     8401 tattttataa aattgcttta aatcatagtg ataggactta cgcgaattg
//
LOCUS       DATRME010000081         8332 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRME010000081 DATRME010000000
VERSION     DATRME010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            534..785
                     /locus_tag="VBC23_001773"
     CDS             534..785
                     /locus_tag="VBC23_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843700.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
     gene            804..1058
                     /locus_tag="VBC23_001774"
     CDS             804..1058
                     /locus_tag="VBC23_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6843701.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(1395..1589)
                     /locus_tag="VBC23_001775"
     CDS             complement(1395..1589)
                     /locus_tag="VBC23_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843702.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            1588..1782
                     /locus_tag="VBC23_001776"
     CDS             1588..1782
                     /locus_tag="VBC23_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843703.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(1833..2009)
                     /locus_tag="VBC23_001777"
     CDS             complement(1833..2009)
                     /locus_tag="VBC23_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843704.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(2045..3634)
                     /locus_tag="VBC23_001778"
     CDS             complement(2045..3634)
                     /locus_tag="VBC23_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6843705.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            complement(3695..3763)
                     /gene="shp2"
                     /locus_tag="VBC23_001779"
     CDS             complement(3695..3763)
                     /gene="shp2"
                     /locus_tag="VBC23_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6843706.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            3851..4699
                     /gene="rgg2"
                     /locus_tag="VBC23_001780"
     CDS             3851..4699
                     /gene="rgg2"
                     /locus_tag="VBC23_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEO6843707.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            4848..5669
                     /gene="mutM"
                     /locus_tag="VBC23_001781"
     CDS             4848..5669
                     /gene="mutM"
                     /locus_tag="VBC23_001781"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6843708.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            5660..6253
                     /locus_tag="VBC23_001782"
     CDS             5660..6253
                     /locus_tag="VBC23_001782"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6843709.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            6377..7078
                     /locus_tag="VBC23_001783"
     CDS             6377..7078
                     /locus_tag="VBC23_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843710.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERYRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            7303..>8332
                     /locus_tag="VBC23_001784"
     CDS             7303..>8332
                     /locus_tag="VBC23_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843711.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFLFLSAVPGYLIYIGESYSKFCFYHLDRPLLRYNFYRERENILATLK
                     IRFLYSIKL"
BASE COUNT         2661 a         1251 c         1507 g         2913 t
ORIGIN      
        1 cgataggaat gattttatca ttaggtcgta tcatttctcc tgaacactct atgtttaata
       61 aacttagaaa gtgaaatgaa ttatatttaa aatttgtttt caacagagcg gatttgacca
      121 tctgttgttt gccatgcctt tcataatttt tcagtaatta tgatagttgt tctgttctcc
      181 caatcataaa actttaaata agattctatt agttcttgcc tcactctcct taagtttctg
      241 gtaaaaatcg aaaagttacc agactttaaa ggggagtttt ttatttgtca acagttagta
      301 gaaaaacgat atattgctcc atttagatta attatggaga taaccaaata atagcttgtg
      361 ataagaagat acctttgaag tttcagaaac aatctgtttt ccatcctgct atattcatct
      421 ttgccaaagt tgcaacaaca caataataac aattgcttgg gcattgggtg attagtttac
      481 tatcaatagg gttagagtgg agttggagta tatggattgg cattaatctt ataatgatga
      541 ttctgttgag ttatgtgcta acaaaaactg agccaatcag taactaccaa gagtcatcat
      601 ctaacctcaa aaaatggcta tttgcaatag gtgcttttag cgttttattt tgctgggcac
      661 tactagtaaa aacatttttc ccaataaggc aaaaccagga gggattaata gctgatatga
      721 atcaggtcaa tcactacttt cgagttgtct tattattgac tatctgtctt attgccccaa
      781 tttaagaaga gataacgttc aggttgtttg tcatgagaac atttgggaac tccctgagtg
      841 gtcccttggt tgtgatctta tcttctatac tatttagctg gtctcactta catggtctat
      901 ctgtcgtcga ctttgttgtc tattttggta tgggtttgat tttcgctagt ttgcaccact
      961 atacaaaatc cattcattat tcgattggcg agcatattgt ttggaatagt ttgtcatata
     1021 ttttttattt tctagcattt ttgcttgatt tactctaagt aataagattg agagactaat
     1081 cattactaag acatttttgt cagtttaaaa gagctttttg gctcttttgt tattcgtttt
     1141 tttaatgaat aaaatgttat tattttattg ttaactaaat aaagttcgta actttatatg
     1201 atttatatag aattacgagt tttttgttag ataaatatga gaaaatatag tatgacgaat
     1261 tcctaaataa aaaataatga aaggaacatg atgttaaaag atgtgaatgt tggaatgagt
     1321 catttattag ttagaggtgc tattattcta gtagtaggga ttgtttttct gttgcaacag
     1381 attcagtaag tacttcaagt gggagctttg gaataggtta tcgagaagaa aagacccaga
     1441 cgttctcttt ggatcagctg tcccaagtga ttttgtattg cggggagccc aatactgtta
     1501 gatttactta tacaaatagc ttctatatga tgaaagatat ttatatcgct ctggatggtg
     1561 ctggtagctc tttgaccact catgatcatg ctgttcctaa tgattaaacc agattatata
     1621 aggtatttca aagtcattga cgatatgcat gataccatgt accaacagac tgtttttcat
     1681 gttcaactgc aagcttggaa atacaccatg aaatcgattg atcaattagt tgtagatgat
     1741 ttgaatcata tcagtaatga gaaaaccact ttggttaaat aaaaaggaac actgtcaaaa
     1801 atggcagtgt tcctttttat tttaggagag agctaagatt ggtcatctaa tgggatttcc
     1861 acatgttcaa ctgtcatagt aatgacaggt ttgataacac aatctctgtc tttagggata
     1921 gtctcctgtt gcagaggcgc ctcatcgctc caaattttct tgtcagaaca attggaatgt
     1981 agataatttg aatcagtctt tttagacatt tcatatcctt tccaggctca atacaatttt
     2041 tgagttatgc accttgatgg cgatcagctg gaatatcttt agctaatgca ttgtattgac
     2101 tatccaaaag atgatcagca tccattattg ttttaccaac aaggaaatat ttattgcttt
     2161 ttatattatt atcccatgtt gtatcaacat aatatttaac gccattaata gtaacaatgt
     2221 tccaagcgtg atttccacct ccagcttgac cagttacata ccatactgat aagccagcag
     2281 cagccatatc tttaaacatt actgcaaagg cttgacaaac accacgttta tctttgtaga
     2341 gtgcttcagg agcatggata ctgataccac tagcagtttc acgagtggtt gccagttctt
     2401 cagtcgcgta agtgtaatta ttaaccaagt ggtcatgaat tactttagct ttttcataat
     2461 cacttgtgat atttttgtta gtaatatttt ctttgacaaa agaatctaca aactgtttat
     2521 attctttatg taatcctacc atgtcacctt taagagtata ggtgattgtg atgtcgcttt
     2581 ttacaataat gcgttggatt tgtccaccaa gagtataagt gctaatgtta cttttggcat
     2641 tgaattcagt tgctgttcca agtgtatctc caatttcccg gatgatgctt ccgagatttt
     2701 tttggtattc gtcaacttgt tcttgagttt taaactccat ttgaacactg atgtgtggta
     2761 cttggttgct aatattattt cgtaaaactt cttttaaact atcgtaatcg gcaatattag
     2821 ctgactcagg ctgtgatgcc tttgcatcag aggtagcctg tttttcctca ctcttagtta
     2881 cttcttgttt attgcctgac gcttctgatt gagtagtttt ttcttctact ttgtttgcag
     2941 gaacagaagt attttcgcta actttgagag tagcaagttt agaagtctca gtttcaatag
     3001 ccgctgttga tagggcatct acttcttgtt gaaggattgc catagcagct ttgtaattat
     3061 taacttcagc tacaagaggt tcaatttgat ttctgagtga atgaaataaa ttttcagctt
     3121 cttgtataga attcacagag ttgtagttta cagttgctaa aatagcttga attttgttaa
     3181 caacctgatt acgttgcccc acgagctgag ctgctttatt ttgacttgtc tgaatcttat
     3241 tatgattttg gtcaatcaga ttttgaacag tttggttaat gacattacgt tgattaacga
     3301 tagcattata gttactggga gagagatgac cagctttcac ctggttaaat aattgctcat
     3361 ctttttgttt aaataatgtt atagctctat ttaactgttc aatagttgga ttagcattta
     3421 gttgagtgaa tgagttgaca ttatcaacaa caaattgatt gagcaaacga tgttgttcct
     3481 tgatgtaact aataagctgc ttttcattat tttgcttact gatatagtga tactcactgg
     3541 tggtgtcatc agctttaacc tcatagttaa tagttgatag tgaactgata attaaagctg
     3601 cggcaaagcc actaataatt tttttaatct tcattagttc tcctgttata aactaaataa
     3661 aatgggattc cataacaacc atttgtgggg agttttatcc accaacgata attaggatat
     3721 ccatgattaa tgtgaacaac aaagctttgt taattttttt catgactatc tcctttctga
     3781 ttttctattt tgtacttgta ttatataatg gatttaaagg gtcttttaaa ttgttgtgaa
     3841 agtgggaaaa atggaaaaag aattaggaaa aacactaaga agattacgta aaggaaaaaa
     3901 agttagtatt tcttctttag cagatgagca cctatccaag tcacaaatct caaggtttga
     3961 gagaggagag tcagaaatta cttgtagtcg tttgcttaat attttagaca aactcaacat
     4021 cactattgat gaatttgtgt cgattcattc taaggcgcac actcattttt ttatcctttt
     4081 aaatcgagta agaaaatatt gtgctgaaaa aaatgtaact aaattagtgg ctctcttaga
     4141 ggatcataac cataaagact atgaaaagat tatgattaag gctcttattt tttcaattga
     4201 tcaatcaata gagcctaatc aagaagaatt agcacgctta acagactatc tttttacggt
     4261 ggagcagtgg ggatattatg aaattattct cttaggaaat tgctcacgtc tcattaatta
     4321 caatacttta tttttattga ccaaagaaat ggttaattcg tttgcttatt cagaacaaaa
     4381 taaaaccaat aaaatcctag ttacacactt agcaattaat tgtctaatca tcagcattga
     4441 tcactcttac tttgagcata gccactactt aattgataag gttagatcat tgttacagga
     4501 tgaagttaat ttctatgaaa aaactgtttt cttatatgta actggatact atcatttaaa
     4561 acttggtgat acttcttcag gaaaagaaga tatgaggaaa gctttacaga tttttaaata
     4621 tttaggtgaa gattcatttt acaatactta taatgaacat tattgtcaaa aagtacttgg
     4681 caataataaa gaatgctaat gagtgcaagt ttatgagtaa tttttaaagc ttttgtctca
     4741 aaatgataat aagaaaggaa aatagaaatc gaatggtcag gtgacatcaa gaacgtttgg
     4801 aaattattgc tctaagcaat gactattgat ttttagacta tatatttatg ccagaattac
     4861 cagaagtaga aaccgttaga aaaggactag aaagacttgt agttaatcaa gaaatagctt
     4921 ctataacgat taaagtccct aaaatggtga aaactgattt gaatgacttt atgataagtc
     4981 taccaggaaa aacaattcaa caggtattga gacgagggaa gtatcttctt tttgattttg
     5041 gtgaaatggt tatggtatca catttacgca tggaaggaaa atatctactc ttcccaaata
     5101 aagtaccaga taacaaacat tttcaccttt atttcaaatt gactaatggc agcacattgg
     5161 tttaccaaga tgtccgtaaa tttgggacat ttgagttggt tcgtaaatca tctctcaaag
     5221 attattttac tcaaaaaaaa ttaggtcctg agccaactgc tgacacattt caatttgagc
     5281 ctttttcaaa aggcttagca aactccaaaa agccaataaa acctttacta ttagatcaga
     5341 gattagtagc aggacttgga aatatttacg tagatgaagt cttgtgggca gcaaaaattc
     5401 acccacagcg tttggctaat caattgaccg aatctgaaac aagcttatta cacaaggaaa
     5461 ttattcgcat tttaacttta ggcatcgaaa aaggtggctc cacgattaga acatataaga
     5521 atgctcttgg agaggacggt acgatgcaaa agtatttaca ggtctatggt aaaactgggc
     5581 aaccgtgtcc aaggtgtggt tgtttaatta agaagataaa agtaggaggg agagggacgc
     5641 attattgccc tcggtgtcaa tgcttatgac aaaaataata ggactgacag gagggatagc
     5701 ttctggaaag tcaacggtaa caaaaataat acgagaatca ggttttaaag tcatagatgc
     5761 ggatcaagtg gttcataaat tgcaagctaa gggtgggaaa ctttaccaag ctttattaga
     5821 atggttgggt cccgagatac ttgatgctga tggtgagttg gatagaccaa agctttctca
     5881 aatgattttt gctaatccag acaatatgaa gacatcagct aggctacaaa atagtatcat
     5941 tcgtcaagag ttagcatgtc agcgcgacca attaaaacaa acagaagaga tatttttcat
     6001 ggatattcct ttattgattg aagaaaagta tataaaatgg tttgatgaga tttggttggt
     6061 atttgttgat aaagaaaaac aattacaacg attaatggcc cgtaacaact acagtcgaga
     6121 agaagcagaa ttacgacttt cacaccaaat gcctttaaca gataaaaaaa gtttcgctag
     6181 tcttattatt gacaataatg gtgatttaat aactttaaaa gagcaaatat tggatgctct
     6241 tcaacgttta tagaactact aaatggataa tgtatttatc cattttttgt ctttattaca
     6301 ctagttttaa aaaacttgcg attaaaccac agtaatagta caatgatata aaaggtttac
     6361 tttcaaagga gtttttatgg agttagtaat tagagatatt cgtaagcggt ttcaggaaac
     6421 agaggtcttg agaggagcaa gttaccgatt ttattcaggt aaaataacag gggtcttagg
     6481 taggaatggt gctgggaaaa caactttatt taatatactt tatggggatc ttgcagctga
     6541 caacgggacc atttgtttat tgaaggataa tcacgagtat cctcttaccg ataaggatat
     6601 tggtattgtt tattccgaaa actaccttcc agaattttta acagggtatg aatttgtaaa
     6661 attttacatg gatttacatc cttcagatga tttaatgaca atagatgatt atttagattt
     6721 tatggaaata ggacaaacag agcgttatag aattatcaaa ggatattctg atggaatgaa
     6781 gagtaagctc tcattaattt gcctgatgat ttctaagcca aaagtaattt tactagatga
     6841 gccactgact gcagttgatg ttgtatcaag tattgcaata aaacgccttt tgttggaatt
     6901 aagtgaggat catattatta tattatcaac tcatataatg gccttagcag aagatctatg
     6961 tgatattgtg gctgtattag acaaaggaaa actccaaaca ttagatattg atcgtaaaca
     7021 tgaacaattc gaagagcgtc ttcttcaagt gttgaaggga gatgaatatg acaagtaaag
     7081 cattaatatg gtctatatta cagattgata gtgctagagc ttataatagt tttttatatt
     7141 accttcagaa aatcccctgg ttgggaagaa aaatccctaa ccgatggtat aaaaggtgta
     7201 gtactattta ttcgtatcat tttctttttc ttaagaggtt ttggtcgttc cataggttac
     7261 gtactatatt gttatgtttt atcatttttt gttagaagtg gcatgaagca gttaaatatt
     7321 ttaccgcaag aaggttttac cctaacttta acattgatgg tgattacctc tcttttaatt
     7381 gtcataaatt tctatataaa acttatagaa cctaatgata tgcaacaaac tcatatgatt
     7441 cgccttttca gaattgagcc atcgaggtat ttccaatccg ctgaactttt agaagccagt
     7501 agtgaactat tttttagaag tttgagcttt ggcatatttt tccatttaaa tcactttgct
     7561 ttttggcatg gtttgacttt tgctttattt ttagcaggta gtcgccttgg aatcaaggtg
     7621 ctctgtttac ctttatatgg tagaggtaaa gaccaagaga atccagagtc tttactaaat
     7681 attttatttt acttagggat tggtatagga gtcttccttt ctctcttttt ccttcttctt
     7741 ggcaaacagt tctctccata tccatttttt tcatggtaca ccggtattgc aggacttatc
     7801 atatggttag ttagttacta tcgattaaaa acttattcta aacttaacca cttgacctac
     7861 cttaccctaa cgcatgagac gatgaaggag aaaattgcaa agattgataa tatagagctg
     7921 cttgggattg aaatgaaaga tagggatata aatgtcagtg aactttctcc cctgctattt
     7981 gaaaatttat caggtatttc atatttaaac gctatctttt taaaacggat gagtagatac
     8041 ttgcaaagga aaattttggt acgtttagct gttttaagtg ttctgtttgg aatagaactc
     8101 cttttcctgc tattttttaa agaaaagata aatattccga tgaatgagca tagtttttca
     8161 aaattcctat ttttgagtgc agttccagga tatctaatat atatcggtga atcctattca
     8221 aaattttgtt tctatcattt ggatcgtcca ttattaagat ataatttcta tcgtgaaaga
     8281 gaaaatatct tagcaacctt aaaaatacgt ttcctttata gtataaaact ta
//
LOCUS       DATRME010000082         8301 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRME010000082 DATRME010000000
VERSION     DATRME010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..824)
                     /locus_tag="VBC23_001785"
     CDS             complement(<1..824)
                     /locus_tag="VBC23_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEO6843712.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELL"
     gene            990..2132
                     /locus_tag="VBC23_001786"
     CDS             990..2132
                     /locus_tag="VBC23_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6843713.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            2157..3413
                     /locus_tag="VBC23_001787"
     CDS             2157..3413
                     /locus_tag="VBC23_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6843714.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            3514..4578
                     /locus_tag="VBC23_001788"
     CDS             3514..4578
                     /locus_tag="VBC23_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6843715.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(4633..4968)
                     /locus_tag="VBC23_001789"
     CDS             complement(4633..4968)
                     /locus_tag="VBC23_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6843716.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(5157..6185)
                     /gene="queA"
                     /locus_tag="VBC23_001790"
     CDS             complement(5157..6185)
                     /gene="queA"
                     /locus_tag="VBC23_001790"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6843717.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            6338..7018
                     /locus_tag="VBC23_001791"
     CDS             6338..7018
                     /locus_tag="VBC23_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6843718.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            7169..7870
                     /locus_tag="VBC23_001792"
     CDS             7169..7870
                     /locus_tag="VBC23_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6843719.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIILVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(7942..>8301)
                     /locus_tag="VBC23_001793"
     CDS             complement(7942..>8301)
                     /locus_tag="VBC23_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6843720.1"
                     /translation="TSQNVYQKEVETLFTALDQLEKHLSDNHYLVGEQFTEADIRLFT
                     TLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRIYNLPGIAETINFDHIKKHYYGSHKT
                     INPTGIIPAGPNLDWTI"
BASE COUNT         2662 a         1290 c         1621 g         2728 t
ORIGIN      
        1 aataacagtt caagatcaag gtcgtcgacg aggttgacct tttgttgacc ttttaaagct
       61 ttcagggcta agatagacgt ttcatatgat ctttttaaaa gtgataaaga tgatttttgt
      121 ccgataccaa ctttatagag acccttttcg cattcaaact ttgataggat atttggataa
      181 taatagtcaa acatctggtc agaaagtaaa agccagtatt gattgggata attaaaggta
      241 taaatagcag tgtcaagttt atcaaaaaag ttcaagagtg acatttcaga ttgacttaag
      301 agtaattttt gtttttgact attgatttca aaagtgagta gacgatagtc agttttttct
      361 gataggtggt attggtttaa aaattgattg aggtaatagt agtctaactc tctgccttca
      421 acaagatggt gtaagacaat agaagcttcg ttttttcgcc cgaaatccag ataatctaat
      481 tcatgctctt ttaagatgag tcgagtcatt ttttgagcaa gtaaagcata ttttcctact
      541 tggttggggt ttccactaat cccaatagta gctaataatt cacaattata atagaaagga
      601 atattgatcc ctgcttgtgt cccaaaataa gattcttgat ctgttacttc aatcatttgt
      661 cctgtctgtg ctacttttaa accaatttca tggaattctc caacacgttt tggattggta
      721 ctagcgaaaa tgattccttt ggtattaata aaattaatat cttgttgaca aacatctttg
      781 atagaggtaa cgatttgttg tgcgagttgt tttcttaatt tcattcagct taccttttgt
      841 tatatataac aaatatcata tcataaaaac gctaacattt gttctatagt ctatattttt
      901 tgttatttga ttttgttact ataattgagt gatataaaat agtgaaaaaa taacaaagat
      961 tcacaaaata ttttcaagga ggttttcaaa tgaaggtagt agtagctatt gattctttga
     1021 aagggagtct ttcttcttta gaagctggca atgctataaa ggagtcaatt aatgaagtaa
     1081 tcccaggggc ggatgtggaa gtacatccat tagcagacgg gggagaagga actgttgaag
     1141 ctttgacgct aggtatgggg gggacgatcg aaataatccc tgttaagggg cctcttggag
     1201 aaaaagtaca tgcgagttat ggtattattc cacagcgtca gttagctatt attgaaatgg
     1261 ctgcagctgc tggtattacc ctaattgcta ctgaagagag aaatcctctc cacacaacca
     1321 cttatggagt tggagaaatg ataaatgatg ctataagtaa aggctgtagg cactttatta
     1381 ttggtattgg aggtagtgct actaatgatg gtggggcagg tatgctacaa gctttgggat
     1441 atgcattatt agataaagat aatcaagaaa tcagtttggg agcacaagga ttggcagatt
     1501 taaaatctat ctctactgat aaggttattg aggaattaaa agaatgcgat tttaaaattg
     1561 catgtgatgt aacaaatcct ttatgtggtg ctcaaggttg tagttcgatt tttggaccgc
     1621 aaaaaggtgc tgatgaagat atgattacaa aaatggatac ttggttaagt aattatgcta
     1681 ctttagctac ttcagttagt gaaaaagcag atgcaactat tgaaggaaca ggtgcagcgg
     1741 gtgggcttgg atttgccttt ttagccttta caaatgccac cttagaacct ggtattgata
     1801 ttattctatc agaaattaac atagaaaaag cgatttctga agctgattta gtagttactg
     1861 gtgaaggtcg tttggatggg caaacggtga tgggaaaagc tccaattggc gtagcaaaat
     1921 tagctaaaaa atatggtaag aaagtagtgg cattctctgg atctgtcacc gaagatgcta
     1981 tactttgtaa tcaacatggt attgatgctt tttttccaat cgtacgacgc cttatcagct
     2041 tagatgaggc tatgtctaaa gaggtagctt ataaaaatat gaaagaaact gctactcaag
     2101 tctttagatt aatcaatttg tataatcatt aatattgaaa ggaattctga taaattatgg
     2161 aagctacatt tacaacaaca ggggctttaa taggattagc gcttgctatt ttactgatta
     2221 tcaaaaaagt tcatcctgcc tatagtctca ttcttggagc attagttggt ggtttgattg
     2281 gtggcggtga tttagttaca attgttaata ctatggtatt aggtgcgcaa gggatgatgt
     2341 catccatatt acgcatttta acgtcaggga ttttagcggg tgctttaatt aaaaccggat
     2401 cagctgaaaa aattgcagaa agtatcatta aaaaattagg acaacagcga gccatcacag
     2461 ctttagccat cgcaacaatg attatttgtg cagtaggtgt ctttattgat attgcagtta
     2521 ttacagttgc tccgattgca ttagcaatag gtaaaaaagc taatctttct aaatctagca
     2581 tactgttagc aatgattggt ggtggtaagg ctgggaatat tatttcaccc aatcctaata
     2641 caattgctgc ttcagaagca tttaaggttg atttaacaag cttaatggta caaaatatta
     2701 ttcctgcaat tgcagcttta gtagttacga ttatattagc aaagattgtt tccaagaaaa
     2761 ataacgatat ttcttatgat agtgaggagc aagtgggctc tgatctgcca gcattcttac
     2821 cggctatttc tggtccactt gtggttatct gcttattagc tcttcgtcct ttatttggaa
     2881 tcactattga tcccttgatt gcactgccgt tgggtggtct aattagtatt ttagcaacag
     2941 gttacctaaa agaaacggta ccgtttgttg aatacggttt atcaaaagtt gttggcgttt
     3001 caattctttt gattggaaca ggtacacttt caggtattat taaagcatct aatctacaat
     3061 ttgatatgat tcatctcctt gaatttttaa atatgccgac ctttatcctt gccccactct
     3121 caggtatttt tatgggagca gcaacagcat caacgacttc tggaacaact attgcttcac
     3181 aaacgtttgc tgaaaccttg attaaatcag gggtaccggc ggtttcgggt gcagctatga
     3241 ttcacgcagg tgcgactgtt ctggattcgc tacctcatgg atccttcttc catgcgacag
     3301 gcggagctgt caatatggct ataaaagaac gtatgaaact aattagctat gaagctttga
     3361 ttggattaac tagcacaata gttgctgtcg tttactattg tttctttggg taaaatttgg
     3421 atgaatacgt gatgtttatt atcactgttt gaatagaatt ctcattgatt ttttctaatt
     3481 ttcttactat aataaattag taggagaggt gttgtgaaac aaacaaagaa agaatttatt
     3541 ttaggattaa ccattattat tacccttact ttattaatca tgctgatttt tttagtaatg
     3601 tttaataagt ccagtaactc taagttgaag cagacaaaag atagaagaat attatctact
     3661 tcaaccacaa ggacatccag tagctcacat gatcaaaaaa ttgtagaaaa gtctcttgaa
     3721 aaaaagaaaa tggtcatgga acaagatatg gaattgatga atgcctatag gctctattat
     3781 gattatgccc atttgtcctt gaaagaggtt gttctggcct atatgtctga atatggcatt
     3841 gaaaaaaata gtgttgcttt ttcttataaa aatttaaaaa caggacaaag ggacgctatg
     3901 aatgatactc aggctatgac agcagggagc acctataagt tacctcttaa catgttggta
     3961 gtagatgcag tggtcaaggg caagttgtcc atggaagaac gctttgatat taccgagact
     4021 gactatgaat atattggtga acataataat tatgttgctg ctttctcggg ggcaatgtct
     4081 attgatgata tgcaaaaata ctctttagtt tattcagaaa atacacccgc ctatgcttta
     4141 gctgagcgta ttggtggcat ggatagtgct tattcaaagt ttggtcgtta tggccaatct
     4201 aaaggagaca tcaaaaatat acaaaaaaac ggtaataaag tgacaacaga ttactatatt
     4261 caagtgctag actatctctg gaaacatcgt aaaaagtata atagtttaat tacttattta
     4321 gaagaagctt ttccaacgga ttattatcgt gctttgatac catctgatgt tgttgttgct
     4381 caaaagccag ggtatgtccg tgaggcgttg aatgttggag caattgtcaa agaagaagtg
     4441 ccttatatcg ttgctattta tactgcaggt ttaggtggct ctactcagga agattcagaa
     4501 attaatggag tgggactata tcagctagaa caattatgtt ttgttattaa tcaatggcat
     4561 cgtgttaata tgaattagaa ataaatctat caaaataata aaaacctctt agattaacta
     4621 tctaagaggt ttttataaat gtgattttat aacttgatta agtgaagaaa gggcagaaac
     4681 agttttttga taagattcag gactaatttc tgagaaaaaa tacccgcacg attgcaccgc
     4741 attgacaacg gcatcttgtg actcaatacc tttgttactt agatgaataa tgagttgcct
     4801 tttgtcatct cttggttttt ctcgtacaat ccacccacga ttttccatac gctttaaaac
     4861 tggagttagg gtattcgaat taagagatag tgcataacta agttccttca tatgccgatg
     4921 atcctcttcc caaaggacta acaagataac gtattggaga taagtcaatt gaaattcatc
     4981 caaagctttt tggtaaaatt tattgaaaag tcgatgtgtt tcactaaccg taaaacataa
     5041 tcgctcgtct aaaatacttc gattactcat tatcatattg tagcattttg gaggcgttat
     5101 agtatgtcaa tcgtgttaaa aaacgataaa actttttcct aattgaaagc ctattcttac
     5161 ttaacaaaca tagcatcacc aaaactgaag aaacgatatc gttcttcaac tgcatggttg
     5221 taagcttcta gtacaaagtc atgtccagca aatgctgata ctaacatgac aagagtagac
     5281 tttggtaagt gaaagttggt tgaaaaagcg tctacaacct tgaactgata accaggcttg
     5341 ataaaaatat ttgtccaccc tgagtcggct tttaattcac cattgaattt agaaccaatt
     5401 gtctctaatg tgcgaataga agttgtacca acagctacaa tacgtccacc agattcttta
     5461 actgcattga gagtatcagc agcttcttta gataattggt aaaactcaga atgcatttcg
     5521 tgttcatcaa gattatcaac tgaaacagga cggaaagttc caagtccaac gtgaagcgtc
     5581 aaatagacaa gcttcacacc ttttgtttca attttttcca atagttcttt tgtaaaatga
     5641 agaccggctg ttggtgcagc agcagaacca ttttcttttg catacacagt ctgatagcgg
     5701 tcacgatctt ctagtttttc atgaatatat ggcggaagtg gcatttcgcc caaactttca
     5761 aggacttcta gaaaaatacc atcatatgag aattctacaa tacgcccacc atgttctaat
     5821 tcctttgtga ctgttgctat taaccgacca tcaccaaatg aaactctgtt ccctacgcgt
     5881 aagcgtttag cgggtttagc aagcacttcc cattgatcgc cctctgtatt tttgagaagt
     5941 aataactcta catggccgtg tgtatctggc ttttcaccat aaaggcgagc gggcagaaca
     6001 cgtgtgttat tcattactaa agcgtcacca ggcttgagtt catctaagat atgatcgaaa
     6061 tggctatctg tcatcgtctt attcttatga tcaatgacta atagtttaga agcgtctctt
     6121 ttttcaagtg gtgtttgggc aataagttct tctggtaaat aaaaatcaaa atcgtttgta
     6181 ttcatgattt ctcctatcta taagtaagct tttctattat atcacgttcg gtaaaaatga
     6241 cccaactaat cttcttattt agaatgatta taaaaaataa ttgtaatcat tctaaataag
     6301 tggtataata attgtcagaa agtgagaagt agagaagatg aaagaagcaa aaaatttttc
     6361 ccaacaattg aatattttaa gagctggtgt tcttggtgct aatgatggta tcatctctgt
     6421 agctggtgtt gttattggtg ttgcgagtgc aactcataac ctttggatta tttttttatc
     6481 agcagcttcc gcgattttag ctggtgcatt ttctatggct ggcggggagt atgttagtgt
     6541 ttctactcaa aaagatactg aacaagcagc agttgcgcgt gaagagaaac tactagaaaa
     6601 taatccagaa cttgccaaaa aatccttagt tgatatctac ttagcaaaag gagaaagtca
     6661 cgaacatgct caatggctag ttgacaaggc tttttcaaag aatgctattg agcatttagt
     6721 tgaagaaaaa tatggtattg agttcggtga atatacaagc ccctggcatg ctgctatatc
     6781 tagttttata gcttttgcca tcggatcaat ttttccaact ataactattc tgttattacc
     6841 attttcggta cgaattgtag gaacggtaat aattgttatt gtttcactat tatcaactgg
     6901 ttatgtaagt gctaagttag gccaagcccc tacagtacct gccatgcgtc gaaatgtgat
     6961 gattggttgt ttgacaatgt tggcaaccta tgtcattgga caattatttt caatataaaa
     7021 tattaatcgt ggagttattg tctaaactag aatggacagt aattcttttt agttttataa
     7081 cgaaattaag aagtttttat tgacaaaaat tggtatatac catataatat aatcataaag
     7141 gtctatacca aataaaagga gagtaaacat gagagttatt acagttaaga acgacattga
     7201 ggggggaaaa attgcattta ctcttttaga agagaaaatg aaaactggtg ctcaaacttt
     7261 aggattggca acaggaagta gcccaatcac cttttacgaa gagattgtaa aaagtaacct
     7321 tgatttttca aatatggttt ctattaacct tgatgaatat gttggaattg ccgcaagtaa
     7381 tgatcaaagt tactcttatt ttatgcataa acatcttttt gatgctaaac cttttaagga
     7441 aaataacctt ccaaacggct tggctaaaga tttgaaaaaa gaaataaaac gatacgatgc
     7501 tgtcataaat gctaatccaa ttgattttca aattcttgga attggtcgta acggtcatat
     7561 tggttttaat gaaccaggta caccttttga tattacaaca catgtggttg acttagctcc
     7621 aagtacaatt gaagctaata gtcgtttctt taatagtatt gatgatgtac caaagcaagc
     7681 cttatcaatg ggtataggtt ctattatgaa atcaaaaaca atcatattag tggcgtatgg
     7741 tattgaaaaa gcagaagcga ttgcaagtat gattaaagga cctatcacag aagatatgcc
     7801 agcaagtatc ctccaaaaac atgatgatgt tgtgattatt gtggatgaag cagcggcatc
     7861 aaagttgtaa agtaaataat aaaaaagtaa ggatgaatct caatgagaaa aaattcattc
     7921 ttactttttt attatgcttt attaaattgt ccagtctaag ttaggtccag ctggaataat
     7981 accggtaggg ttaatggttt tatggctacc gtaatagtgt tttttgatat gatcaaaatt
     8041 aattgtctca gcaattccag gtaggttgta aatacgtttt gtataatgcc ataaatgtgg
     8101 gtaatcatgt agcgctttta aattacattt aaaatgacca taataaacgg tgtcaaagcg
     8161 aactaaagtt gtgaaaaggc gaatatcagc ttcggtaaat tgttctccaa caagataatg
     8221 gttatcagat aagtgttttt ctaactgatc taaagctgtg aataaggttt caacttcctt
     8281 ttgataaacg ttctgagaag t
//
LOCUS       DATRME010000083         8131 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRME010000083 DATRME010000000
VERSION     DATRME010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBC23_001794"
     CDS             complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBC23_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6843721.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
     gene            459..2333
                     /gene="dnaG"
                     /locus_tag="VBC23_001795"
     CDS             459..2333
                     /gene="dnaG"
                     /locus_tag="VBC23_001795"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6843722.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKKNME
                     "
     gene            2341..3450
                     /gene="rpoD"
                     /locus_tag="VBC23_001796"
     CDS             2341..3450
                     /gene="rpoD"
                     /locus_tag="VBC23_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6843723.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            3559..3900
                     /locus_tag="VBC23_001797"
     CDS             3559..3900
                     /locus_tag="VBC23_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6843724.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            3990..4844
                     /gene="rfbD"
                     /locus_tag="VBC23_001798"
     CDS             3990..4844
                     /gene="rfbD"
                     /locus_tag="VBC23_001798"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6843725.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            4961..6115
                     /locus_tag="VBC23_001799"
     CDS             4961..6115
                     /locus_tag="VBC23_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6843726.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            6105..7046
                     /locus_tag="VBC23_001800"
     CDS             6105..7046
                     /locus_tag="VBC23_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6843727.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            7048..7779
                     /locus_tag="VBC23_001801"
     CDS             7048..7779
                     /locus_tag="VBC23_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6843728.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            7776..8129
                     /locus_tag="VBC23_001802"
     CDS             7776..8129
                     /locus_tag="VBC23_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6843729.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
BASE COUNT         2736 a         1224 c         1626 g         2545 t
ORIGIN      
        1 taatccgagt cttttattat ttgttagcga gtaagtcgcg gatttcagca agtaattgct
       61 cttgtgttgg ttcaacaact tcaataattt cttcttcagc aggtttctta gccataacct
      121 tgttagcagc tttaacgatg aagaataaag tagtaccaac gattaagaaa ttaataactg
      181 cacttaagaa attaccgtaa gcaacaccat tccatgaaag ctgtgcgata tttgaaacgc
      241 cagcagcttt taatacagga ttaaggataa gcggtgtaat aacgtcttta acaagtgaag
      301 tgatgatagc attaaaagct gcacctaaaa taacagcaac agcaaggtct aaaacattac
      361 ccttaaataa aaattctttt agttctttaa tcatgtgaac aagctcctta atattattta
      421 attcacgttc attatagcag atttcttttg agaaatcctt gaaaaagctt ttgaaaaaat
      481 ttacacagaa aagaaaaaat gatataatca aatgtaaaac tataatgggt tatttctgtg
      541 gaggtcatga cttggcaatt gataaagaaa aaatttctga aatcaagaac agtgttaaca
      601 ttgttgatgt catcggtgaa gttgtcggtt tgacaaaaac aggacgcaat catcttggtt
      661 tatgcccatt tcataaagaa aaaacgccat cttttaatgt tatcgaagat aggcagtttt
      721 ttcattgttt tggttgtggt cgctctggtg atgtctttaa atttgtggaa gattaccaac
      781 atattagttt tttagatagt gtacaagttc tagctgaacg ttcaggaata cctctagata
      841 ctaattttaa ggggcaagtt cctaaaaaac ctaaagctaa tcagtcgctt ttagatattc
      901 atagagttgc ttcgggattt taccatgcct atttaatgac aactaatgat ggtgaacgcg
      961 ctagacaata ccttgccgag cgcggagtga cagaggactt aattaaacat tttcaaatag
     1021 gattatcgcc gggagggcag gattttctat atcgtcgtct ggcaaaagag tttgatgaaa
     1081 aaactttgat gtcttctggt ttgttcaatt attcagaaaa tagcaatcag ttttacgatt
     1141 catttaataa tagaattatg tttccgttaa ctaatgatat tggtgaagtc attgcgtttt
     1201 caggacgagt ttggactcaa gaggacattg atagaaaaca agctaaatat aaaaattctc
     1261 gtgccacccc tatttttaat aagtcttacg agctttacca tttagataaa gcgcgtgctg
     1321 taattaataa agcacacgaa gtttacctaa tggaaggctt tatggatgtt attgcagctt
     1381 atcgtgcagg aattgagaat gttgttgctt ctatggggac cgctttaacc aatgaacacg
     1441 ttagacacct taaaagattt actaagaaag ttgttctgac ttatgatggt gatagagctg
     1501 gtcaaaacgc aattgacaag tctttagaac tgctatcaga tatgactgtt gatatcgtta
     1561 ggatacctaa taaaatggac cctgatgaat ttctacaagc aaattcagca gaagatttta
     1621 aacaactctt agaaaatggg cgcatcagta atacagagtt ttatatccac tacttaaagc
     1681 ctgaaaacac cgataattta caatctgaaa ttgcttatgt tgaaaaaata gcgaaattaa
     1741 ttgctaaatc tccatcgata acagcacaaa attcatatat tacaaaagtg gctgaactct
     1801 tgccagattt tgattatttt caagtagaac aatcggttaa taacgaacga ttgcatcata
     1861 gaagtcaaca gcaagcttca tcttcagtac aaacaagtgc tacagtgcaa ttaccacaga
     1921 caggtaagtt atcagctatc actaaaacag aaatgcaatt gtttcatcga ttattgaatc
     1981 atccctacct gttaaatgaa ttccgtaata gggataattt ttattttgat acaacagaaa
     2041 tacaagtttt atatgagttg ctaaaagaaa gtggtgagat cacgtcttat gatttatcac
     2101 aagaaagtga caaagttaat cgtacttatt atataatttt agaggaacaa ttacctgtag
     2161 aagtatctat tggagagatt gaagccgtcg aaaaggcacg tgaccgttta ttgaaagaac
     2221 gtgatttaag aaaacaaagc cagttaatta gacaatctag taatcaaggt gatgaagaag
     2281 gagccttggc tgcattagaa aatttaattg ctcaaaaaaa aaatatggaa tgaggaacct
     2341 atggcagaga aaaaaggaaa tacaactttt aatgttcaag ttgcggattt tattcgcaat
     2401 cataaaaaac aaggtactgc aattgatgat gaggtaacag aaaaacttgt tattcctttt
     2461 gttttagatg cagatcaaat tgatgatttg ttagaacgat tgacagacgg tggcatttca
     2521 atcacagata aagaaggcaa tccttcaaca aagtatgtgg tagaagggcc aaaacctgaa
     2581 gaattgactg atgaagagct cataggaagt aattctgcta aagtcaatga cccagtacgt
     2641 atgtacttga aggaaattgg tgttgttcct cttttaacga atgaagaaga aaaagaattg
     2701 gctgttgcgg ttgctgaggg agatttaatg gctaaacaac gtttagctga agcaaacctt
     2761 cgtcttgttg tctcaattgc aaaacgttat gttggacgtg gaatgcaatt tcttgattta
     2821 attcaagagg gtaacatggg gctcatgaaa gctgttgata aatttgatta ttcaaaggga
     2881 ttcaaatttt caacttatgc aacatggtgg attcgtcaag ctataacacg tgcaattgca
     2941 gatcaagcac gtacgattcg tattcctgtt catatggttg aaacgattaa taaattggtc
     3001 cgtgaacaac gtaatcttct tcaagaactt ggtcaagatc caactcctga gcaaatcgca
     3061 gaacgcatgg atatgacacc agataaagta cgtgaaatcc taaaaattgc tcaagagccg
     3121 gtatcgctag aaacgccaat cggtgaggaa gatgatagcc atttgggtga tttcattgaa
     3181 gacgaagtta ttgaaaatcc agtagattat actacacgtg ttgttcttcg tgagcaatta
     3241 gatgaggttt tagatactct tactgaccgt gaagagaatg ttcttcgttt acgctttggt
     3301 cttgacgatg gtaaaatgcg tacgttagag gacgtaggta aagtatttaa tgttactcgt
     3361 gagcgcattc gtcagattga agctaaagcc cttagaaaac ttcgccatcc tagtcgtagc
     3421 aagcaactta aagatttcat ggaagattga ttgcttctcg ataatcgttt ttagaatagg
     3481 aagtttagtt tctaataaaa atgatttgca aaggagttat cttaaagacg gtattaatat
     3541 tttcagagag gtattataat gtcagaagta aagcaatata gcgaagaaga agttggaaaa
     3601 ataaaagatc gtatcctaga agctttggag atggttatcg atcctgaatt gggaatagat
     3661 attgttaacc ttgggttaat ttatgagatt cgctttgaag ataacggtcg aactgaaatt
     3721 gatatgactt taacgactat gggatgcccg ttagcagatt tattgacaga tcaaattcat
     3781 gatgttatga aaacagtccc agaggtaact gaaaccgagg tgaaattggt atggtaccca
     3841 gcctggtctg ttgataagat gagtcgttat gctcgtatcg ctttgggaat tcgttcataa
     3901 gataaagagt aaagggatga aatattccct tttttagtgt acaataaacg aagaattaaa
     3961 agttaaactt atttttaaag gagcaaggaa tgattttaat tacaggggca aacgggcaat
     4021 taggtagtga attacgtcac cttttagacg aacgaactca agagtatgtt gctgttgatg
     4081 tagctgaaat ggatattaca aatgccgaga tggtggacaa agtctttgaa gaggtgaagc
     4141 catctttagt ttaccattgt gcagcttaca cagctgttga tgcggcagaa gatgaaggaa
     4201 aagagctaga ctttgcaatc aatgtcactg gtacagagaa tgtcgctaag gctgctgcta
     4261 aacatgatgc aacacttgtt tacatttcga cggactacgt gtttgatggt gaaaaaccag
     4321 tggggcaaga atgggaagta gatgatctgc ctgatcccaa aacagagtac ggtcgtacaa
     4381 aacgtatggg tgaagagtta gttgaaaaat atacttcaaa attctatacc attcgcacag
     4441 cgtgggtatt tggtaactat ggtaaaaact ttgtctttac gatgcaaaac cttgcaaaaa
     4501 cacacaaaac tctaactgtt gttaacgacc aacatggacg accaacatgg acacgtactt
     4561 tggctgagtt tatgacttat ttagctgaaa atcaaaaaga ttttggttac tatcatttat
     4621 caaatgatgc taaagaagat acaacatggt atgattttgc ggttgaaatc ttaaaagaca
     4681 cagatgtgga ggttaagcct gttgattcta gtcaattccc agcaaaagca aaacgtcctt
     4741 taaattcaac tatgagtttg gaaaaagcga aagcaacagg atttgtgatt cctacttggc
     4801 aagatgcttt aaaagagttc tacaagcagg aagttaaaaa ataagatcaa aacaaagagt
     4861 agtaaggcta gagaatcctt ttagcttact gctcttttta gctaactttt ctaatttatg
     4921 gtataattgt atggattgtt tagctagaat ggagaagatg atgcaagatg ttttcattat
     4981 aggaagtaga gggttgccag ctcgttacgg tggttttgaa acttttgttt cagaattgat
     5041 taatcatcaa aaaagttccg acataaaata ccatgttgca tgccttagtg ataaagaaca
     5101 tcatactcat tttaactttg ctgacgctga ttgttttact ataaatcctc cccaattagg
     5161 gccagcacgt gtgattgctt atgatattat ggccattaat tatgcccttg acttggttaa
     5221 gacacatgat ttaaaagagc ctatttttta tattttagga aatacaattg gtgcctttat
     5281 ttggcatttt gccaataaaa tacataaagt cggtggctta ttgtatgtta atccggatgg
     5341 tttagagtgg aagcgatcaa agtggtctcg tcccacacag cgttatttaa aatacgccga
     5401 aaaatgtatg actaaaaatg cagacctaat tatttctgat aatattggta ttgaaaatta
     5461 cattcaatct acctactcta atgtgaagac aaggttcatt gcttacggta cagagattaa
     5521 ttctaggaaa ttatcgtcag atgatccacg tgtcaaacag ttgtttaaaa aatggaatat
     5581 taagtctaag ggttactatc taatcgttgg tcgatttgtc cctgaaaaca attatgaaac
     5641 ggctattagg gagttcatgg cttcagatac taagcgtgat ttagttatta tctgtaacca
     5701 tcaaaataac ccctactttg aaaagttgtc cttaaagaca aaccttcaac aagataaaag
     5761 agttaagttt gtaggtacgc tctatgaaaa agatctgctg gattatgttc gtcaacaagc
     5821 ctttgcttat attcatgggc atgaagttgg cggtactaat ccaggactgc ttgaggcttt
     5881 agctaatact gatttgaatc ttgttctaga tgttgatttc aacaaatcag tagcaggtct
     5941 ctcaagtttt tactggacta aaaaagaggg ggatttagct aagcttatta acgatagtga
     6001 tcaacaacag gatttatcta cttacggtga tcgagcaaaa gccattattc aagaaaacta
     6061 tacttggaaa aaaattgttg aagaatatga ggatttgttt ttaaatgaaa gttaatattt
     6121 taatggccac ctacaatgga gagaagtttc tggcgcagca aatcgaaagt atccaaaagc
     6181 aaacctttaa agagtggaac ctcctaatca gagatgatgg ttcaagtgac aagacatgtg
     6241 atattattag aaactttact gctaaagatt ctcgaattcg tttcattaat gaaaatgagc
     6301 accataattt aggagttatt aaatcatttt ttacgctagt taattacgaa gttgcagatt
     6361 tctatttttt tagtgatcaa gatgatgtat ggttgccaga aaagttatct gtaagtttag
     6421 aggcagcaaa gcataaagca tctgatgtac cattacttgt ttatactgat ttgaaagttg
     6481 ttaatcaaga attaaatatt ttacaagaca gcatgattcg ggctcaatcg caccatgcca
     6541 atacgacatt attaccagaa ctaactgaga atactgttac tggtgggact atgatgataa
     6601 atcatgcatt agctgagaag tggtttactc ctaatgatat cttaatgcat gattggtttt
     6661 tagcactctt agcagcaagt ttaggagaaa tcatttattt ggatttacca actcaactat
     6721 accgtcaaca tgacaataat gttttggggg cgaggacaat ggataagaga tttaagatac
     6781 ttcgtgaagg tcctaaatct atatttacaa ggtattggaa acttatccat gactcacaaa
     6841 agcaagcttc cttaattgtt gataagtatg gtgatattat gacagctaat gatcttgagc
     6901 tcattaaatg ttttataaaa attgacaaac agccttttat gacaagatta aggtggttat
     6961 ggaaatatgg ttatagcaaa aatcaattta agcatcaagt tgtgtttaaa tggttgattg
     7021 caacaaatta ttacaataag agataagatg atgaaaaaaa ttttactagt gatacctgct
     7081 tataatgaag aaggtagtat tgcaaagaca gtccagacta ttgtagattt taaggcgtct
     7141 cgctcactac cttttgagct ggattatatt gttattaacg atgggtcaac cgatggtact
     7201 ccagagttat tagatagatt gggtttaaat catattgacc ttgttcaaaa tctaggtatt
     7261 ggtggctgtg tgcagacagg atacttatac gctaatcgta atcattacga tgttgccgtt
     7321 caatttgatg gtgatggtca acatgatatt agaagcattg aagatgttgt tatgccaatc
     7381 ttaaatgacg aagcagattt tgttattggt tctcgctttg ttgataaaaa acatcaaaat
     7441 tttcaatcga cagcaatgag gcgtcttggc ataaatttaa tatctgctgc tataaaatta
     7501 acgacgggac acaaagtcta tgatacaaca agtggttata gggcagccaa cgcggcgcta
     7561 atagcttatt tatcgtgtca ttatccagta caatacccag agcctgaatc aactgctcgg
     7621 attttgaaaa aaggctatcg tttgaaggaa gtcacagcta atatgtttga acgtgaggca
     7681 ggtacttcaa gtataagttc cctaaaatcg attttttata tgacggatgt tttaacatca
     7741 attattattg cagggtttat taaggaggat gataaatgag tttagcattg caaattgaaa
     7801 tgtttatttt agccattgtc ctactttata tgattataag aatggttaat aaaaacacat
     7861 tttctgttaa acgttcttta ccttggctat tagttggttt aattctagtc tttattagtg
     7921 tatttcctgg ggtggtctca tttatcgctc atcaccttgg attcgcttta acgatgaatt
     7981 tcttgttatt tggtgctatt ttatttctgt tttgtttaga attatttgat actagtagca
     8041 ataccaaaag agatgagcaa attaaacgcc tgattcaaga ggtttctttg ttgaaaaaag
     8101 agatagagga tagcacttta gatgagtaaa a
//
LOCUS       DATRME010000084         8122 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRME010000084 DATRME010000000
VERSION     DATRME010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..733
                     /locus_tag="VBC23_001803"
     CDS             <1..733
                     /locus_tag="VBC23_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEO6843730.1"
                     /translation="IDFKQLEKDIIENNVKLYIFCSPHNPGGRVWTKGEIQKIGDICK
                     RYNVILVSDEIHQDLVLFDNVHHSFNTVDSSFKELSVILSSATKTFNIAGTKNSFAII
                     ENEKLRSDFKKRQIANNQQEISSLGLLATEVAFTKGKQWLKALKMELEGSIEYLYEQL
                     TQKTHIKVMKPEGTYLVWLDFSAYNLTHLEIQEKLRYDAKLILNDGLTFGKEGKKHAR
                     INVAAPRSVIEEAVLRLVTNFRKND"
     gene            843..1472
                     /gene="upp"
                     /locus_tag="VBC23_001804"
     CDS             843..1472
                     /gene="upp"
                     /locus_tag="VBC23_001804"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6843731.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            1597..2187
                     /locus_tag="VBC23_001805"
     CDS             1597..2187
                     /locus_tag="VBC23_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6843732.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            2287..2532
                     /locus_tag="VBC23_001806"
     CDS             2287..2532
                     /locus_tag="VBC23_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO6843733.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            2687..3853
                     /locus_tag="VBC23_001807"
     CDS             2687..3853
                     /locus_tag="VBC23_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6843734.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            3959..4828
                     /locus_tag="VBC23_001808"
     CDS             3959..4828
                     /locus_tag="VBC23_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6843735.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            4831..5784
                     /locus_tag="VBC23_001809"
     CDS             4831..5784
                     /locus_tag="VBC23_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6843736.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            5785..6549
                     /locus_tag="VBC23_001810"
     CDS             5785..6549
                     /locus_tag="VBC23_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843737.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            6549..7259
                     /locus_tag="VBC23_001811"
     CDS             6549..7259
                     /locus_tag="VBC23_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843738.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            7278..7937
                     /locus_tag="VBC23_001812"
     CDS             7278..7937
                     /locus_tag="VBC23_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6843739.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
BASE COUNT         2621 a         1184 c         1702 g         2615 t
ORIGIN      
        1 gattgacttt aaacaattag aaaaagatat cattgaaaat aatgtaaagc tttatatttt
       61 ttgtagtcca cacaatcccg gtgggcgtgt ctggacaaaa ggagaaattc aaaaaatagg
      121 agatatctgt aaacgatata atgttatcct cgtttctgac gaaatacatc aagatttggt
      181 attatttgat aatgtacacc atagctttaa tactgttgat tctagtttta aagaattgag
      241 tgttatttta tctagtgcaa ctaaaacgtt taatattgca gggactaaaa atagttttgc
      301 aataattgaa aatgaaaagt tacgctcgga ttttaagaag aggcaaatag ctaacaatca
      361 acaagaaatt tcttcattgg ggttattagc tacagaagtt gcctttacaa aagggaaaca
      421 atggttaaag gccctaaaaa tggagttaga aggatcaata gaataccttt atgagcaatt
      481 aacgcaaaaa actcatatca aagtgatgaa accagaggga acttatcttg tttggttaga
      541 tttttctgct tataatttaa cgcatttaga aattcaagaa aaacttcgat atgatgccaa
      601 attaatatta aatgatggtt taacttttgg aaaagaaggg aaaaaacatg ctagaatcaa
      661 tgtcgcagct cctaggtctg ttattgagga ggcagttttg cgattagtga caaactttag
      721 aaaaaatgac tagactataa aaaaagtcgc atggaaaggg tttttatgtt ataatacttt
      781 gaatgtaaac aagcattaga atgtttttaa tgctcaaaat taaatagaaa gcggtcatta
      841 taatgggaaa atttcaagtc atttcacatc cacttattca acataaactg tcaatcttac
      901 gtcgcactac aacatctact aaagattttc gtgagttagt tgatgagatt gcgatgttaa
      961 tgggctatga agtgtctcgt gatttgcctt tagaagatgt tgaaatccaa acaccagtag
     1021 cgacaactgt acaaaaacaa ttagctggga aaaagttagc aattgttcca attttacgtg
     1081 caggtattgg tatggttgat ggtttcttaa gccttgtgcc agctgctaaa gttggtcata
     1141 tcggtatgta tcgtgatgaa gagactttcc aaccagtcga atatttagtt aagttacctg
     1201 aagatattga tcaacgtcag atttttgtag ttgatccaat gcttgctaca ggtgggtctg
     1261 ctattttagc agtagattct ctaaaaaaac gtggcgcagc tagtatcaaa ttcgtttgtt
     1321 tagtagcagc tccagaagga gtggcagcac ttcaagaagc acatccagat gtagatattt
     1381 ataccgctgc tttggatgaa aaactcaatg aacatggcta cattgttcct ggactcggag
     1441 atgctgggga tcgcttattt ggtacaaaat aagatttgag atgaataagg cttttctgaa
     1501 gaactcagaa agccttttct taaaaaagtt attatttgac ctttgttgac caaatgatta
     1561 taatagtgac agtgataaaa agaaatggag aaagatatga ttccagtagt tattgaacaa
     1621 acaagtcgtg gtgaacgttc ttatgatatt tactcacgtc ttttaaaaga tcgtattatt
     1681 atgttgacag gccaagttga ggataatatg gccaatagta tcattgcaca gttattgttt
     1741 ctcgatgcac aagataatac aaaggatatt tacctttatg tcaatacacc aggtggttca
     1801 gtatcggctg gacttgctat tgtggacacc atgaacttca ttaaatcgga cgtacagacg
     1861 attgttatgg ggatggctgc ttcgatggga accattattg cttcaagtgg tgctaaagga
     1921 aaacgtttta tgttaccgaa tgcagaatat atgatccacc aaccaatggg cggaacaggc
     1981 ggaggtacac agcaatctga tatggctatc gctgctgagc atcttttaaa aacgcgtcat
     2041 actttagaaa aaatcttagc tgataattct ggtcaatcta ttgaaaaagt ccatgatgat
     2101 gcagagcgtg atcgttggat gagtgctcaa gaaacacttg attatggctt tattgatgct
     2161 attatggaaa ataataattt acaataatag atttaaaaga gttgagttta ccaactcttt
     2221 ttttatttgt tggaattatg ttataatctt agtaattaca gatatgacgc agaaaggaaa
     2281 aaattattga ataaacaaga gcgtttagga atgagcgttt atctttacta taatcgtgac
     2341 gctcgtaaat tatataaata cggagatgtt cattatcatt ctcgtagatt aagatatcta
     2401 gtcatttatg ttaataaaga ggatattgtt agcgttagta aagagataaa acatttaaaa
     2461 tttgttaagg atgttcggtt atcagcaatt gatgatattg atcaggattt tgttggaaac
     2521 ttatatcgtt aagatattta ctagtagtta ttagaaagaa ggagttcaga tgtccttctt
     2581 ttttatttag tttctataat tttcaaaaaa acaatttaaa aactattgac aatattctga
     2641 taattctgta ttattttagt taccattttc aaaaaaggag aaaaatatgg aaaagagact
     2701 tagtttagga gcgcttgttt tagctagtac agttctttta gcagcatgtg gaaatgtcgg
     2761 aggaggagca tcttcgacag gaacaaaaat agggaaagat attaaggtag ggtataactg
     2821 ggaattatca ggaaacgttt cttcatatgg aaattcaatg aagaatggag ctgacttagc
     2881 agtaaaagaa attaatgctg ctggaggggt tggcggcaag aagttaaaag tcctatcaca
     2941 ggataataaa tcagaaaatg cagaggcagc aacggttgcc acaaacttgg ttaccaaagg
     3001 agctaatgtc attatcggac cagcaacatc gggtgcagct gcatcttcaa ctccaaaagt
     3061 aaatgcagca gcagttccaa tgattgcacc tgctgcgaca caagacaatt tagtctatgg
     3121 ttctgatgga aaaaccttaa atcagtattt cttccgagct acttttgtcg ataattatca
     3181 aggaaagcta ttgtctcagt atgctacaga caaccttaaa gctaaaaaag ttgttctatt
     3241 ttatgataat tcatcagatt actcaaaggg ggtagcaaaa tcatttaagg aaagttatag
     3301 tggaaaaatt gttgatagta tgacattctc cgctggtgat actgatttcc aagcgtcatt
     3361 gactaagttg aaagggaaag aatatgatgc tattgtgatg ccaggttact ataccgagac
     3421 aggattaata gttaagcaag cgcgtgattt aggtatctct aaaccggttc ttgggcctga
     3481 tggttttgat agtccgaaat ttgtgcaatc ggcaacacct gtaggagctt caaacgttta
     3541 ttatttgaca ggtttcacta cacaaggatc aaccaaagct aaagctttcc atgatcatta
     3601 cgttaaggca tatggtgaag aaccatccat gttctcggct ttatcatacg acgctgtgta
     3661 tatggcagct aaatctgcta aaggagctaa aacttcaatt gatattaaaa aagctttagc
     3721 taagctgaaa gattttaaag gtgtgacagg gaaaatgtct attgataaaa atcacaatgt
     3781 tgttaaatca gcttacgttg tcaaattaga ggatggaaaa acaagtagcg ttaacattat
     3841 ttcagcaaaa taagtattct ctagaaaaat gggagtttaa ttttcagctc ccctttttta
     3901 aaaagtaatt tttcagaata attcgacaat aatatataat agaaagtatg gttataatat
     3961 gcttcaacag ctcgttaatg gacttatctt aggatccata tatgctttac ttgccttagg
     4021 atatactatg gtatatggca ttattaagct gattaatttt gctcatgggg acatctatat
     4081 gatgggcgct ttcatgggct attatttgat taatcatctt catcttaatt ttttcctagc
     4141 tttattaatt gcaatgctgg ggtctgcttt cctaggtgtt gtaattgaat atttagctta
     4201 tcgtccactg agaaaatcaa ctagaattgc cgcgttaatt acagctattg gtgtttcttt
     4261 tcttttggag tacgggatgg tataccttgt tggagcggat actagggcat ttccgcaagc
     4321 cattcatact gttaaatata atttaggacc gataactata accaatgttc agctcatcat
     4381 tttaggtatt gcgctattac taatgcttac tttacagttc atcgttcaaa aaactaaaat
     4441 ggggaaagct atgagagctt tatcagtgga tagcgatgcc gcacaattga tgggaattaa
     4501 tgttaaccgt accattagtt ttacatttgc gctggggtct gctttagctg gagcaggtgg
     4561 agtactgatt gggctttatt ataattctgt tcaacctctt atgggggtaa ctccagggtt
     4621 gaaagcattt gttgcagctg tcttaggtgg tattggtatc attccgggag cggctatcgg
     4681 tggttttgtt attggcattt tagaaacttt agcgactgca cttggtgtgt cagattttcg
     4741 agatggtatc gtttatgcta ttttaattct cattttcttg atccgtccag caggaattct
     4801 tggaaaaaat attaaggaga aggtctaggc atgaaagaaa atatgaaatc aattttatct
     4861 tggttagcta tagtagtgtt aggttatctc ttgattagcg tgctaatatc tatgggtata
     4921 tttaacctct atcatattca aattattgaa acaattggga ttaatgttat tttagcagtt
     4981 ggtttaaacc taattgtagg ttgttcggga cagttttcac taggacatgc aggtttcatg
     5041 gctattggcg cttatgcagt tgctattatc ggcgttaaaa tgccaactta tgtaggtttt
     5101 ttgatagcta tacttgttgg aactcttgtt gcaggaggta ttgccttagg tgtaggaatt
     5161 cctactttga gactaaaagg tgattattta gctatagcta ctttaggtgt tgctgagatt
     5221 atccgtattt tgttggttaa tggaggtgat attacgaatg gtgctgcggg tatcatgggg
     5281 attccgcctt ttacaacctg gtctttagtt tatggggtgg cagttgtgag ccttatcctt
     5341 gcaatgaatt ttttgcgtag tcccttaggg cgtaatacaa ttgctatacg agaagatgaa
     5401 attgcagcag agtctatggg tgtggataca acaaaagtga aagtaattgt ttttgttttt
     5461 ggagctattt tagcttctat cgctggtagt ttacaggctg gctacgttgg aactgttatg
     5521 ccaaaagact tttcatttat gatgtctgtc aatgttttaa ttattgttgt tttaggtggt
     5581 ttaggttcaa tgacagggac agttttagca gcaattttat taggattatt gaatatgtta
     5641 ctacaagact acgcttctgt tcgaatgatt atctatgctt tagccctgat tttaattatg
     5701 atttttagac ctagcggttt attaggaacg aaagagttaa ctctatctca tctatttcgt
     5761 aagacgagta agggaggcaa ttagatggca cttcttgaag taaaaaattt aagtaaacat
     5821 ttcggtggcc taactgctgt aggtgatgtt tcgatgaaat tacataaagg tgaattaatt
     5881 ggcttaatcg gccccaacgg tgcgggtaag acaacgcttt ttaatctctt aacaggagta
     5941 taccttccta gtaaagggac aatatcactt gatggtaaga tattaaatgg gaggaaacca
     6001 gctaaaatag cttctttagg tttaggtaga acttttcaaa atatccgact ttttaaaaat
     6061 atgactgttt tagacaatgt actagtagga ctcagtaatc atcacttatc acatcctatt
     6121 gctagttttt tacgattacc caaatactat catagtgaaa aagccttaag gaaaaaggca
     6181 ctggagctat tagagatttt tggtttgaaa gcatatcaag atgctcttgc taagaacctc
     6241 ccttatggaa aacaacggcg tttagaaatt gttcgtgctt tagcaacaga accaaaaata
     6301 ttgtttttgg atgagcccgc ggcaggaatg aatcctcaag aaactgcaga attgacgcag
     6361 ttaattagtc aaataaagag tgattttgat attactatta tgctgattga acatgatatg
     6421 aacttggtta tgcaagtaac agagagaatt tatgtattgg aatatggtcg tctgattgct
     6481 cacgggacac ctgaagaaat taaaaataat aaacgtgtta tagaagcata cttggggggg
     6541 gagctataat gaccatgttg aaagtagaaa atctatctat tcactatggt gttattcaag
     6601 cggtgaatga tgtctcgttt gaagttaacc aaggtgaggt tgtaacctta attggagcca
     6661 atggtgcagg aaagacatca attttgagga caatttctgg tttagtaaga ccaagtcagg
     6721 gttctatttc ttttatgggg aaacctatac ataagttagc agctagaaaa attgttggca
     6781 atggtttagc tcaggttcca gaagggcgcc acgttttctc aagtttgtct gttatggaaa
     6841 atttagaaat gggagccttt cttcaaaagg atcgtgaaca aaatcaaaaa atgttaaaaa
     6901 aagtgtttga tcgttttcct cgcttagaag aacgtaaaaa ccaagatgca gcaaccttat
     6961 caggtggtga gcagcaaatg ctcgctatgg ggcgtgcatt gatgagtcgt cctaaattat
     7021 tacttttaga tgaacagtca atgggcctag ctccaatttt tattcaagaa atttttaata
     7081 tcattgaaga catcaaaaaa caagggacta cagttctttt agttgaacaa aatgcaaata
     7141 aagctctgac gattgctgat aaagcatatg ttctagagac tgggaaggtt gttttatcag
     7201 ggacaggtaa ggaattacta gtttcagatc aagttcgtaa agcttactta ggtggataat
     7261 atttagagga gtcaggtatg ccagtgaaag attttatgac aaaaaaatta gtttatgtct
     7321 ctccagatac tacagttgca gaagctgctg atttattgag agaacaccac ttaaggcgtt
     7381 tgccagttgt tgaaaatgat cagctagtgg ggttagtgac agaagggaca atggcagaag
     7441 cacaaccttc taaagcaacc agtctttcta tatatgagat gaactatctt cttaataaga
     7501 caaaaattcg agatattatg attaaagata ttgtgactgt ttcacagtat gctagccttg
     7561 aggacgctat ttatttgatg atgtctcgaa agatcggtgt tttaccagta gttgataatg
     7621 gacaacttta tgggattgtc acggataggg atgtttttaa agccttcctt gaaattgcgg
     7681 ggtatggtca agaaggttat cgcttggtta tattagctga tgaggggata ggggtactat
     7741 caaaagtcct aaatcgtctt tctagtgcaa atttaagtgt taaacgttta gtcattattg
     7801 aacgaaaagc aggtaagaaa gcggtcgaga tacaactaga aggatacgct gacaaggatg
     7861 ttttaaaaca agaactagta tttgatgatg ttattgtgga aactttagaa aagaccatga
     7921 aaaaaccact ttcttaaaaa tatcagctaa attctcaaag taagttccac ctctagtaga
     7981 aatggaattt agtctgataa aaaccaataa aaaccaacga aaatccgtct cagactaagt
     8041 tccgttggtt tttataatac ttgatttcat aggttttcag agttaaaaat cgcaaaaaaa
     8101 gggtacaaga aaacatcctc at
//
LOCUS       DATRME010000085         7951 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRME010000085 DATRME010000000
VERSION     DATRME010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            24..950
                     /locus_tag="VBC23_001813"
     CDS             24..950
                     /locus_tag="VBC23_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6843740.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            1121..1837
                     /locus_tag="VBC23_001814"
     CDS             1121..1837
                     /locus_tag="VBC23_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6843741.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            1839..2672
                     /locus_tag="VBC23_001815"
     CDS             1839..2672
                     /locus_tag="VBC23_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6843742.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(2724..3527)
                     /locus_tag="VBC23_001816"
     CDS             complement(2724..3527)
                     /locus_tag="VBC23_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6843743.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            3693..6143
                     /locus_tag="VBC23_001817"
     CDS             3693..6143
                     /locus_tag="VBC23_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6843744.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            6225..7940
                     /gene="pabB"
                     /locus_tag="VBC23_001818"
     CDS             6225..7940
                     /gene="pabB"
                     /locus_tag="VBC23_001818"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6843745.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         2596 a         1195 c         1576 g         2584 t
ORIGIN      
        1 ttaattttaa aaggagcatt ttgatgaaaa agtggttagt tattgtatca tgttttgtag
       61 catttttagg cttaggtgcc tgtgctaaca agcaggatca agccaaaaac gaaaaattaa
      121 aagtagttgt aaccaactct attttggcag atataacaaa aaatatagcc ggtaacaaaa
      181 tcgacttaca tagtattgta cctgttggta aagatcctca tgaatatgag ccattaccag
      241 cagacgttga aaaaacaact caagcagacc ttgtttttta caacggaatt aatcttgaaa
      301 caggcggtaa tgcttggttt acaaaattaa taaagaacgc taagaagaaa gaaaacaaag
      361 attactttgc ggttagcgat ggagttgatg ttatttatct aaatggtcaa agcggaaaag
      421 gtaaagaaga cccacatgct tggttgaacc ttgaaaatgg tattatttac tcaaaaaata
      481 ttgctaagca attaattgct aaagatccaa aaaacaaagc tacttatgag aaaaacagag
      541 atgcttatgt agctaaatta gaaaaattgg ataaagaagc aaaatctaaa tttaatgcca
      601 ttccagctaa taaaaaatta attgttacta gtgaaggttg ctttaaatat ttctcaaaag
      661 cttatggagt tccatcagca tacatttggg aaattaacac tgaagaagaa ggtacaccag
      721 atcaaattac tagcttagtt aagaaattaa aacaagtaag accttcagct ttattcgttg
      781 aatcaagtgt tgacaagcgt cctatgaaat ctgtttcaag agaatcaggt atcccaattt
      841 acgcagaaat cttcacagac tcaattgcta aaaaaggtca aaagggtgac agctactacg
      901 ctatgatgaa atggaatctt gataagattg cagaaggttt agcaaaataa gagtgagaat
      961 cgacattgaa atcataatga aatcaatgat gtagtattct cgcttaaggc tgagttgtaa
     1021 caatgtcgaa atcctaatct aaaaactgac ttagctttaa taaatacaga gaagtggagt
     1081 ctggttttat aaccagctcc attctacttt tggaggaaaa atgattatta gtaaacactt
     1141 aagcgtaagt tatgataata atttagtgct agaagatatt aatttacgct tagaaggctc
     1201 cggaatcatt ggtattttag ggcctaatgg ggcggggaaa tctacgctta tgaaagctct
     1261 tcttggtttg gttgattcta ctggagaatc agggattggg ggagatttgc tacctttaat
     1321 ggggagagta gcatatgttg aacaaaaaac taatattgat taccaattcc ctataactgt
     1381 tggagaatgc gtttctttag gattatataa ggaacgaggc ctcttcaagc gactatcaaa
     1441 aactgactgg gaaaaagtat cccgagtgat tgatcaagtt ggtttgagag ggtttgaaaa
     1501 tagaccaatt aatgccttat caggtggaca atttcaacgt atgcttatgg caagatgtct
     1561 cgttcaagaa gcggattaca tttttttaga tgagccgttt gtagggattg attccattag
     1621 tgaacaaatc atagttaatt tactaaaaaa attaagtaag gccggaaaat taattttagt
     1681 cgtccatcac gatttgagta aagttgatca ttattttgat caagtaatta ttttgaaccg
     1741 tcatctaatt gcttgtggac caatagatca ggcatttacc cgtgagaatt taagtgctgc
     1801 ttacggtgat gctattttgt taggacaagg agactagaat gtttacgaaa ttttttgaag
     1861 gcttattgac ttatcatttt ttgcaaaatg cttttataac tgcaattgtg attggtattg
     1921 tagcgggtgc agtaggctgt tttattatct tgcgctcaat gtctttgatg ggtgatgcta
     1981 tttctcacgc tgttctacca ggtgttgcta tatcttttat tttagggatt aactttttta
     2041 taggtgctat tgtctttggt ttgttgtctt cgattatcat tacttatatt aaagaaaata
     2101 gtgtcataaa aggagatact gctattggaa ttactttttc gtccttttta gccttaggta
     2161 tcattttaat tggacttgct aatagtacaa ctgacctctt tcatattctt tttgggaata
     2221 ttttggcagt acaagatagt gataaatata tgactattat tgtgggatta attgtactga
     2281 ctctgattac tatcttcttt aaagaattat tattgacttc ttttgatcct gttttagcaa
     2341 aatcaatggg aatgagagtt tctttttatc attatttatt gatgattttg ttaacattag
     2401 ttgcagtaac agctatgcag agtgtgggaa caatattgat cgtagcactt ttaattacac
     2461 cagctgcaac agcatatctt tatgttaaaa gtctcagaac aatgttgttt ttatcatctg
     2521 cattaggagc agtagcatct gttctggggt tatatatcgg ttatacattt aatattgccg
     2581 caggatcaag tattgtttta acatctactt ttatgttttt acttgctttc ctattttctc
     2641 ctaaacaaag tttgtttaaa aagaaacaat agtgttgtta aaaaatgaga ctagaaaatg
     2701 ttctagtctc atttttgata gaattattta aatttgtagt ctttaataac tttgatactt
     2761 gtaatagtaa ttttttcttt tggttgatct gattttgtaa cttctaccga agcaatttta
     2821 tccacagttt ccatgccaga gattacttgt ccaaagacgg tataaccacc atctaaactt
     2881 ggatttcctc cctctgaata ggctttaata atcaccttag gaactttttt atctgacagt
     2941 tgtttggaat gatcttgctg gctttgattg ataaagaatt ggctgccatt agtatcagct
     3001 ccagcatttg ccattgctaa acttcccctg atattgtaga ggtaagggga tatttcatta
     3061 acaaaaccat ttccagaatc tttcttttta tctttgctat tccatataga ttttccaccg
     3121 gtcccgtctc cattagggtc accagattgt atcataaaat ctttgataac tctgtggaaa
     3181 cttaacccat tatagtatcc ttcttttgca tgtgttagaa aattttcaac agctaaagga
     3241 gcatattttg gaaataactt gatatttata tcacctttag aggtttttat aagcacttgt
     3301 gcctcgtttt tggcaacagc cttgtccaat tgaggaaaag cttttttatc agcttttaag
     3361 gcttgcttag tttttttgtt tagttgttca gtagcttcct ttgaagagtt ttcagctagt
     3421 ttttggtcaa cataacggtc tccttttaga gaacgctcaa ttgactcgca accacttaaa
     3481 gttaatatac tgacacaggc taaaccaaga taaataattt ttttcataca tactccttga
     3541 ttaagttatt aagatgatta tattttatca taaattttta aataatttat aaaagtaaat
     3601 agtaaaccta ttgggggact tgttgaaatt atgatagaat agtatagcat gagttgatag
     3661 agaaagtgag gaacaagtat ttcacttgaa atatggtatt tatggcaaat aagaaaaaaa
     3721 caaaaggaaa gaaaaccaga agacctacta aggcagaaat agagcgtcaa agagctattc
     3781 aaaggatgat tactgctctt gttttaacaa ttattctctt ctttggtatt atcagattag
     3841 gtatttttgg tattacagtc tataacgtca tccgttttat ggtaggtagc ttggcttact
     3901 tatttattgc ggcaacttta atctaccttt atttctttaa atggttgcga aagaaagata
     3961 gcttagtagc aggttttttg atagcttctt taggattatt gattgagtgg catgcttacc
     4021 ttttctcaat gcctattttg aaagataaag aaattttgcg ttcaactgct cgattaattg
     4081 tgtctgattt aatgcaattt aaaatcactg tttttgccgg tggaggtatg ttgggtgctt
     4141 tgatttacaa gccaattgct tttctctttt ctaatattgg tgcctatatg attggtgttc
     4201 tcttcatcat tttgggtctc tttttaatga gttctctgga agtttatgac atcgtcgaat
     4261 ttattagagc ttttaaaaat aaagtggcag agaagcacga gcaaaataaa aaggagcgtt
     4321 ttgctaagcg agagatgaaa aaagcaatcg ctgaacaaga gcgcatagag cgtcaaaaag
     4381 ctgaagaaga agcttattta gcttcggtta atgtagaccc tgaaacgggt gagattctag
     4441 aggatcaagc tgaggacaat ttggatgatg cgctaccacc tgaggtaagt gaaacatcaa
     4501 ctccggtatt tgagccagag atccttgctt atgagacatc gcctcaaaat gatcctttac
     4561 cagtagagcc gacaatttat ttagaagact atgattcgcc gattcctaat atgagagaaa
     4621 atgatgagga aatggtttat gatttagatg atgatgtaga tgatagtgat atagaaaatg
     4681 tcgactttac acctaaaacg acactggttt ataaattacc aacgatagat ttatttgcac
     4741 cagataagcc taaaaatcaa tccaaagaaa aggatttagt ccgaaagaat atcagagttt
     4801 tagaagaaac atttagaagt tttggtatcg atgtaaaagt agaacgtgct gaaattggac
     4861 catcagttac taaatatgaa attaaaccag cagttggagt tcgtgtgaat cgtatttcaa
     4921 atctatctga cgacctagct cttgctcttg cagcaaaaga tgtgcgtata gaagcaccaa
     4981 ttcctggaaa atcattaata ggtattgaag ttcctaactc agaaattgca acggtttctt
     5041 tccgcgaact ttgggaacaa tctgatgcca atcctgaaaa ccttttagaa gtaccactag
     5101 gaaaagctgt taacggcaat gctcgcagtt ttaacttagc tagaatgccg catcttttgg
     5161 tagctggttc aactggttca ggtaaatctg tggcagttaa tggaattatt tcaagtattt
     5221 tgatgaaggc acgtccagat caagttaagt ttatgatgat tgatcccaaa atggttgaat
     5281 tatctgttta taatgatatt ccacatttat taatccctgt tgtaaccaat ccgcgtaaag
     5341 caagtaaggc actccaaaaa gttgttgatg aaatggaaaa tcgatacgag ttatttagca
     5401 aaattggtgt gcgtaatata gcaggttata atacaaaggt tgaagagttt aatgcttcct
     5461 ctgagcaaaa acaaatacct ttgcctttaa tcgttgtcat tgtagatgaa ttggctgact
     5521 tgatgatggt tgctagtaaa gaagttgaag atgctattat tcgtttgggg caaaaagcac
     5581 gtgctgcagg tatccatatg attcttgcaa ctcaacgtcc atccgtagat gttatttctg
     5641 gtttgattaa agcaaatgtt ccgtcgcgta ttgcatttgc tgtttcaagt ggtactgata
     5701 gccgtacgat ccttgatgaa aatggtgctg aaaagctctt gggacggggt gacatgctct
     5761 ttaagcctat tgatgagaat catccagtac gactacaagg ttcctttatt tcagatgatg
     5821 atgttgaaag gatcgttggt tttatcaaag accaagccga ggctgactat gatgatgcct
     5881 ttgatcctgg agaagtatct gaaacagata acggctctgg tggtggcggc ggagtacctg
     5941 aaagtgatcc tctttttgaa gaagccaagg gactcgtttt agagacgcaa aaagcaagtg
     6001 cctcaatgat tcaacgccga ttgtctgttg gtttcaatag agcaacaaga ctaatggaag
     6061 aattagaagc agcgggggtt attggtccag cagaaggaac caagccacga aaagttttaa
     6121 tgactccaac tccgagtgaa taagaaaaaa ctaatctgac aagaaattgt cggattagtt
     6181 ttttatgtta taatgaagca aatagatgaa gaagaaggtt acgaatgcat attgagactg
     6241 ttattgattt caaagaatta ggaaaaagat atcgttttaa aaatcctaca aaagaattaa
     6301 tagctgatac tttagaacaa gtcttagaag tgataaaaga agttgattat tatcaatctc
     6361 aaaattatta tgttgttggt tatttatctt atgaagcatc tgctgctttt gattcacatt
     6421 ttaaagtttc tcaacagaag ttggctggag aacatctagc ttattttaca gtacataaag
     6481 attgtgagaa cgaagctttt cctttaagtt atgaaaatgt tagattagca gataattgga
     6541 ctgctaatgt ttctgagcaa gaatatcaag aggcaattgc taatattaaa ggacaaatta
     6601 gacaaggaaa tacttatcaa gtaaattata cactagagct tagccaacaa ttatgctcgg
     6661 atccctttag tgtttatgaa cgcctaatgg tagaacaagg agcaggatat aatgcttata
     6721 ttgcctacga cgataaaagg attttatctg taagcccgga attgttcttt aagaagaagg
     6781 atgaagtctt aacaactaga ccaatgaagg gaacaagcgc tagaaagcct acttatcaag
     6841 aagatgtggc ggagcgagat tggttggcaa atgatcctaa aaatcgttct gaaaatatga
     6901 tgattgttga tttattaaga aacgatatgg gccgtatctg tgatgttgga acagttaagg
     6961 taaaaaaact atgtcaagtg gagcagtatg caactgtgtg gcaaatgaca tcaactattg
     7021 aaggagtttt atcgccagaa gtgacactta tgtctatttt tcaagctcta tatccttgtg
     7081 gatctatcac tggagctcct aagatatcaa caatggctat tattaatgag ttggaaaaac
     7141 ggccaagagg tatttattgt gggacgatag gactttgcat gccagacgga caagctattt
     7201 ttaacgttcc tattcgcaca gtacaaatga aaggtcaaca agcctattac ggcgttggtg
     7261 gaggtattac gtgggagagc cagacagatt ctgaatatga agaaacccgt caaaaatcag
     7321 ctgttttaac acgtgtcaat ccaaaatttc aattaataac tacaggaaga gtgactgaaa
     7381 ataaacttct tttctctcaa caacatgtcg agagattagt tgaatcagcg tcttattttg
     7441 cttattcttt tgataaaagt aaattcgaaa gggaactgaa aaaatacctt catcagctag
     7501 atgagaagga ttaccgcttg aaaataatgc ttgataaaac tggtaaggta acgtttgagg
     7561 taaaacaact agtgaattta tcaaaaaagt ttttaacggc agaggtggtc gtacaagact
     7621 accctattaa attatccccg tttacttatt ttaaaacttc ttatcgccca catattattg
     7681 aaggtcagaa tgaaaagata tttgtatctc ctgaggggtt gctattggaa acaagtattg
     7741 ggaatattgt tttagaaaaa aatggaaggt ttttaacccc agatttatca gaaggagggt
     7801 tgaatgggat ttatcgtcgt catctcctta aaaaccaaaa agtaattgaa gcaccactaa
     7861 ctttaaaaga tttagaatca gccgatgcta tatacgcctg taatgctgtt agagggcttt
     7921 atcctctaaa cctaaagtaa aaagagttgg a
//
LOCUS       DATRME010000086         7919 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRME010000086 DATRME010000000
VERSION     DATRME010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(28..1092)
                     /locus_tag="VBC23_001819"
     CDS             complement(28..1092)
                     /locus_tag="VBC23_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6843746.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            1321..2604
                     /gene="aroA"
                     /locus_tag="VBC23_001820"
     CDS             1321..2604
                     /gene="aroA"
                     /locus_tag="VBC23_001820"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6843747.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            2597..3109
                     /locus_tag="VBC23_001821"
     CDS             2597..3109
                     /locus_tag="VBC23_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6843748.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            3166..4539
                     /locus_tag="VBC23_001822"
     CDS             3166..4539
                     /locus_tag="VBC23_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6843749.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            4640..5995
                     /gene="rlmD"
                     /locus_tag="VBC23_001823"
     CDS             4640..5995
                     /gene="rlmD"
                     /locus_tag="VBC23_001823"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6843750.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            6103..6324
                     /locus_tag="VBC23_001824"
     CDS             6103..6324
                     /locus_tag="VBC23_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843751.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            6442..7179
                     /gene="aphA"
                     /locus_tag="VBC23_001825"
     CDS             6442..7179
                     /gene="aphA"
                     /locus_tag="VBC23_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6843752.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            7500..>7919
                     /locus_tag="VBC23_001826"
     CDS             7500..>7919
                     /locus_tag="VBC23_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6843753.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKT"
BASE COUNT         2671 a         1178 c         1527 g         2543 t
ORIGIN      
        1 atagaattga tttgataata aacagttcta ccattgcaat ccagcagcag cgatttctgc
       61 ttttgaagcg tattgtccat ttggacgatg ccaccttcct ctatagtcac gtttgtagcc
      121 ttgactagtt tgttcttgta ctagagtttc ttgttttttc tgagtttcag ctgcgagcct
      181 tgcttgctcc tctttttgct tcctttgagc ttcagcagca agccttgctt gttcttcttc
      241 cagttttttc tgagtttcag cagcagcttt ttcctcttca actttctttt ctgcaagtcg
      301 tttttcttct tcgtgcttag cgataagttt tctttgttct ttagctgata agacagtatt
      361 ttgagccttt ccgctgaggt agtcaatggt agcatttggt gaggtattct ctaaggtaac
      421 agtgctgatg ccgaattgat cttgagtgga tttgtttccg ataaaaatag gtaataattt
      481 cccagtctta tcaataccaa catattttaa ttcaattttt cttggaatta gttcattatt
      541 ttgataaatt ggtgttactt tataatctaa gtagtagttt ttatgagtcg aaagccaagt
      601 ctttagttgt ttttcataga atagcatgct ctcagggtta gatgaattcg tgctattgta
      661 gtttccagta tttagccaat tcgtcattgg aactaaattc tttctttcat tgtttagtcc
      721 actaaattgg tggcaaataa gacggcctcg gctcattagc caagcttttt gagtaccatc
      781 attataatag aatttaaaat tgtgccatcc aacaggggtt gtttttaaac gttttactct
      841 ctttttcttg ggttcatctt taacttttag ctgaatatgt gcgaatgtgg cacgaccttt
      901 gtcatccaac tctcctaaaa ccaattgtcg tttgtgttta aaaggtaaag taccattctc
      961 ttttgtttta tactcttgag cagtaacaat attttctgtg gtatgatttg tagctaggtt
     1021 tattggtgtg taaagcgaaa taaccgttag taaggttgca attcctaatt ttataaactg
     1081 ttttcttttc atctgttaac ctctaaaata acataaaata gtagctgttt atttttaaat
     1141 gcattctgaa aattaaaatg tgatttttgt aacaatatta taacataatg atgtatgaca
     1201 tgtgaagtta atttataaaa gatgttacag ctatatagag gagctatagt ataaataaaa
     1261 aattcaaaat atagcatata ttatgataca ataatagcaa aatagatttt gggagttatc
     1321 atgaaattac tgactaatgc aaatactctc aagggaacca tccgtgttcc tggcgataaa
     1381 tcaataagtc acagggctat tatttttggg agtatatctc agggagttac ccgaattgtt
     1441 gatgtcttaa gaggcgagga tgttttatca actatagagg cttttaaaca aatgggggtt
     1501 ctaattgaag acgatggcga gataatcact atctatggca aaggatttgc aggtttaaca
     1561 caaccgaata atctcttaga catgggaaac tctggtactt caatgcgtct gatagcaggt
     1621 gtgttagctg gtcaagagtt tgaagtgacg atggttggag acaatagttt gtcaaagaga
     1681 cctatggatc gtatagcatt gcctttaagc aaaatgggag ctagaatttc gggagtaact
     1741 aaccgtgatt taccaccatt aaaacttcaa ggtacaaaaa aattaaaacc tattttttac
     1801 catttacccg tagcttctgc acaagtaaaa tcagctttaa tttttgcggc tttacaaact
     1861 aagggagaat ctctcattgt agaaaaagag caaacaagga atcatactga ggacatgatt
     1921 cggcaatttg gtggacattt ggatattaaa gataaagaaa ttcgactcaa tggcggtcag
     1981 agcttagtag gacaagatat aagagttcct ggtgatattt cgagtgctgc cttttggata
     2041 gtagcgggtc ttattattcc taacagtcat attattttag aaaacgtagg tataaacgag
     2101 acacgaactg gaatccttga tgtcgtcagt aaaatgggtg gaaaaattaa gttatcttcg
     2161 gttgataatc aagtaaaatc agcaaccctt acagttgatt actctcatct acaagctact
     2221 catatttctg gggctatgat tccacgtctg attgatgaat tacctattat tgctctttta
     2281 gcaacacaag ctcaaggcac cacagtcata gcagatgctc aggaattaaa ggtgaaagag
     2341 acagatcgga ttcaggtcgt tgtcgaatcg ctaaaacaga tgggtgctga tattactgct
     2401 acagcagatg gtatgattat acgtggtaat acccctcttc acgctgcttc acttgattgt
     2461 catggagatc atagaatagg aatgatgata gctattgctg ctctcttggt caaagaaggc
     2521 gaagtagatt tatctggtga agaagctatt aatacaagct atcctaactt tttagaacat
     2581 ttggagggat tagttaatgc ctaaggtatt attgggattc atgggagttg gcaagactag
     2641 tgttgccaac tgcttagaaa atgaagtaat tgatatggat agtctgattg aaaaacatat
     2701 tggtatgtca atttcccgat tttttacaga agaaggagaa gcctcctttc gtgctttaga
     2761 atctcaattt ttaaacgaac ttttaaagaa aaaaaatgaa ggacttgtga tagctagtgg
     2821 aggaggtatt gtactccttg aagaaaatcg tcgtctttta actttaaatc gtcataataa
     2881 tattctttta acaggaagtt ttgaagtctt atatcatcgt ataaaaaaag atgagaagaa
     2941 tagaagacca ttatttttaa accattcaaa agaagaattt tatgatattt atcaaaaacg
     3001 catgttactc tatagtggtt tgtctgatat gattattgat accgattatc ttacaccaca
     3061 aaaaatagct actgtaattg gagagtcact ggttaattta tctaactaag ttatcacagt
     3121 ttggtagtat gaactattat tattgaaagt aaatcacctg aaaatatgtc aagaaataat
     3181 tacggtcagc taaatcatca tgaagagcta cgatataact atcttttaaa aaacatccat
     3241 tatcttaatg aacgtgaaaa gatggaattt cagtaccttc attataaaaa aacagcagta
     3301 cgaccacaga gaaggacaga aagtccacca actaattctt attatgagga accttattct
     3361 gattcttatt atcaagatga cgatttttac tcagagcctc agctaacctc acaaggttta
     3421 ccaatttatc aagaagagag agctccgaag aagaaaaaac aaagggccag aaaagaaaaa
     3481 cagagggtga aggttatggc gcctttccct ccaaaggcta taaccccccc tagaaagaaa
     3541 aaaaaattta agggtttcct aaagttcata ggtattatat tactaattgt tttatcaggt
     3601 atggttttca tgtttgttaa aggaatgcgt gatgtgaata atgggaagtc acattatagc
     3661 cctgcaatta ttgaagattt taagggaaaa gatgctgttg atgggactaa tatcttaatt
     3721 ttaggtagtg ataaacgtgt cagcgagaga tctactgatg cgagaacaga taccataatg
     3781 gttgctaatg ttggtaacaa ggataataag gttaagatgg tgagttttat gcgagatctc
     3841 ttagtcaata ttcctaatta tagtacggaa ggttactatg atatgaaatt aaatgcgtct
     3901 tttaatttgg gagaacaaga taatcataaa ggagcagagt atgttcgaca aacgttgaaa
     3961 aatcattttg atattgatat aaaatattat gttatggttg actttgagac atttgcagat
     4021 gctattgata cactgtttcc taatggtgtc aagattaatg ctaaatttgg acttgtcggt
     4081 ggacaatctg ctgattcagt taaagttcct gatgatttaa ggatgaaaaa tggcgttgta
     4141 cctagccaaa aaattaaggt tgggattcag tacatggatg gaaggaccct tctaaattat
     4201 gctcgtttca gaaaagacga tgatggggac tttggtcgaa cacaacgtca acaacaagtt
     4261 atgagagcta ttgtctctca aataaaagac ccacgtcgtc tctttacagg ttctgctgct
     4321 atcggtaaag cctatgcttt aacttcaagt aatttatctt atagctttgt cctaacagat
     4381 ggcattccaa ttttatcaga tgctaaaaat ggtattaagc aaatgacaat tccaagagaa
     4441 ggagattggg tagatgatta tgaccagtat ggtggtcaag ggttaacaat tgattttgct
     4501 aagtacaaaa agatcctaaa gaaaatggga ctgcgttaac atttttaatt atgatataat
     4561 agagtgattt attgtcactc tatttttatc tttagtgtat aaactaaaga cttcaaatga
     4621 tagatataaa aggaaaatta tgttacataa aaatgatatt attgaaactg aaattagcga
     4681 cattagccat gagggaatgg gtattgccaa agtagatggc tttgttttct ttgttgaaaa
     4741 tgctcttcca ggtgagatta ttaagatgcg cgtcttgaaa ttacgtaagc gtataggata
     4801 tggaaaagtt gaggaatatt taacgacttc tcctcaccga aatgaaggat tagactatac
     4861 ctatttacgt acagggattg ctgatttggg acatcttact tatgaacaac agctgttatt
     4921 taaacaaaaa caagtagctg ataaccttta taaaattgct catatatctg atgttttagt
     4981 agaacctact cttggtatga caatcccttt ggcatatcga aataaagctc aggtaccagt
     5041 tcgtcgtgtt gatggacagc tagagacagg ttttttcaga aaaaattcac atactctggt
     5101 atccattgaa gattatttaa ttcaggaaaa agagattgat gctctgatta attttacacg
     5161 agatttatta agaaaatttg atgttaaacc atatgacgaa gagcagcaat ctggtttaat
     5221 tagaaatctc gttgttcgtc gaggacatta tacagggcaa ttaatgttgg ttttggtgac
     5281 aacacgtcct aaaatattcc gcattgacca aatgattgaa aagttggtgt ctgcctttcc
     5341 ttctgttgtt tcaattatgc aaaatattaa tgatcgtaat agcaacgtta tttttgggaa
     5401 agaatttcgt actctatatg gttctgatac tattgaggac caaatgttag gaaatactta
     5461 cgctatatca gcccaatctt tttaccaagt taatactgaa atggctgaaa aactgtacca
     5521 aaaagcaatc gatttttcag atttaaactc agaggatatc gttattgatg cctattcagg
     5581 tataggaaca attggcttat ctgttgctaa acaagtaaaa catgtttatg gtgttgaagt
     5641 tgttgaaaaa gcagtaagtg atgccaaaga aaatgctact cgaaatggga taactaacag
     5701 tacctatgtt gctgattcgg ctgaaaatgc aatggcaaag tggcttaaag aaggtataaa
     5761 accaactgtt ataatggttg atccaccacg aaaaggatta acggagagtt ttgtatattc
     5821 tgctgcccaa acaaaagcag ataaaatcac ttatatttct tgtaattcag cgacaatggc
     5881 gcgtgatatc aaactatttg aagaattagg gtatcattta gtaaaaattc aaccagtaga
     5941 cttattccca atgacacatc atgtggagtg tgtggctctg ctcgtaaaag cctagaaact
     6001 ttttaacgaa aggaatatta aaaagcctta gataatcgac tgacctatta ccaaaaaatc
     6061 gaaggggctc tacttgccat tgcacaaaca ccaggaaata gtttgccaac tatgcagctg
     6121 ttagatgagt tagttcttcc aaaagacatt accatagcag atgtcagtca aagtatccaa
     6181 gaatttgaaa tagaaagaga tgcccttcaa gaattttttg acgatatgct gaagacttat
     6241 acaacctacc aagaaatgaa agattatatt aaatctcgtg aagcgaacac tcctaagcaa
     6301 gagacagaag aaaaatcact ttgaagactg tagacttaac ataagtaaaa aataaccata
     6361 tatgatatcg ctttcattct tatgtttttt gtgataaact aattaaagaa ataattttta
     6421 tactttatta tggaggttct tatgaagaaa gtgttagtga gtagtctttt ggttttaggg
     6481 attacgataa cgttacaaac agtagttgag gctaaggggc caaaagtagc ttatacacaa
     6541 gagggaatga ctgctctttc ggacacaaat aaagataaag tcactactat ttctattgac
     6601 gagattcaaa aaagcttaga aggtaagaag ccgattactg ttagttttga tattgatgat
     6661 acactgcttt tcagtagtca atattttcaa tatggtaaag aatatgtaac tcctggatcg
     6721 tttgattttc ttcataaaca aaaattctgg gatcttgttg caaaacgagg agatcaagat
     6781 tccattccca aagaatatgc taaaaaatta attgctatgc atcaaaaacg aggagataaa
     6841 attgttttta taacaggtag gacaagaggg tcaatgtata aggagggcga ggttgataaa
     6901 acagctaaag ccttagctaa agattttaaa ttagacaaac caattgctgt aaattataca
     6961 ggcgataaac ctaaaaagcc atacaaatat gataaatcat attatattaa gaaatatggt
     7021 tcagacattc attatggaga tagtgatgac gatattcatg cagctaggga ggccggtgct
     7081 agaccaatta gaattttaag agcacctaat tctacaaatc tacctttacc agaagctgga
     7141 ggctacggtg aagaggttct cgaaaattca gcttactagt atctttatta gttaacatag
     7201 tattatctat caatttttag atttgtaata agaagtgcta aaaaagtagc aaatcaatga
     7261 tatgctaata agggaagttg gagtagttta gattgattat taatgactga tcttctctcc
     7321 tctcggtaac aagaagtttt tctaacaaat gcctgtctaa aaaaattgaa tcataatttt
     7381 ttaataactc ttttggcgtg aacaaggaat ttgtaatcag tcatctaatt ctataataca
     7441 atactttggg gttgtttccg tatcattatt aataatatgt atcagaaagg agatgttttt
     7501 tgagagttta tgaaaataaa gaagagttga aaaaagaaat aagtaaaaca tttgagaaat
     7561 acattatgga atttaataat attccagaga atctaaaaga taaaagaatt gatgaagttg
     7621 atagaactcc agcagaaaac ctttcttatc aggttggctg gaccaacttg gttcttaaat
     7681 gggaagaaga tgaaagaaag ggacttcaag taaaaacacc atcggataaa tttaaatgga
     7741 atcaacttgg tgaattatat cagtggttca cagataccta cgctcattta tctctgcaag
     7801 agttgaaagc aaaattaaat gaaaatatta attctatcta tgcaatgatt gatttgttga
     7861 gtgaggaaga attatttgaa gcgcatatga gaaagtgggc tgatgaagcg actaaaaca
//
LOCUS       DATRME010000087         7680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRME010000087 DATRME010000000
VERSION     DATRME010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            74..412
                     /locus_tag="VBC23_001827"
     CDS             74..412
                     /locus_tag="VBC23_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6843754.1"
                     /translation="MRRVSVSHDLKELLLELKFQQERMLKGLHIKNTDQLIFYDYRFG
                     MISNDAVNKFLASSLEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQ
                     LIETYGHLLL"
     gene            complement(548..618)
                     /locus_tag="VBC23_001828"
     tRNA            complement(548..618)
                     /locus_tag="VBC23_001828"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(584..586),aa:Thr,seq:ggt)
     gene            complement(689..1523)
                     /locus_tag="VBC23_001829"
                     /pseudo
     CDS             complement(689..1523)
                     /locus_tag="VBC23_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            1834..2088
                     /locus_tag="VBC23_001830"
     CDS             1834..2088
                     /locus_tag="VBC23_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6843755.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            2197..2508
                     /locus_tag="VBC23_001831"
     CDS             2197..2508
                     /locus_tag="VBC23_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843756.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            2776..3345
                     /locus_tag="VBC23_001832"
     CDS             2776..3345
                     /locus_tag="VBC23_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843757.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            3443..4027
                     /locus_tag="VBC23_001833"
     CDS             3443..4027
                     /locus_tag="VBC23_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843758.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            4024..4590
                     /locus_tag="VBC23_001834"
     CDS             4024..4590
                     /locus_tag="VBC23_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6843759.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            4590..7241
                     /locus_tag="VBC23_001835"
     CDS             4590..7241
                     /locus_tag="VBC23_001835"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6843760.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(7459..>7680)
                     /locus_tag="VBC23_001836"
     CDS             complement(7459..>7680)
                     /locus_tag="VBC23_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843761.1"
                     /translation="IYLYLIAKESILYFESLDENSHLQLKELLDYNDEWPFDIFDDMD
                     SYTFLYTDIYIEEDSPYHFKNWFVPQFYL"
BASE COUNT         2488 a         1323 c         1564 g         2305 t
ORIGIN      
        1 aaatagtatt tatacatatc gaagatattc ttcaataaaa aatgacttta caaagcctaa
       61 aacaaggact tcaataagga gagtttcagt aagtcatgat ttaaaagagt tattgcttga
      121 actaaaattt caacaagaaa gaatgctgaa aggtttgcat ataaaaaata cagatcaatt
      181 gattttttat gactataggt ttggtatgat atcaaatgat gctgttaaca aattcttggc
      241 aagctcattg gaaaaattag aaatagaatc taagatgtca tcaacaggtg ctagacatac
      301 ttatggaagt tatttgttgg ctaacggagt agatatttgg gcggttgcaa aattaatggg
      361 gcataaagat atcaaacaat taatagaaac atatggtcat ttgcttctgt aagtggaaga
      421 aaaggaaaat gatcagcttt taaaaattct taataataaa ggtagttttt aaaatgacta
      481 tcaagaacaa attaagaaca aaaataaaag aaaccctatt ataataaggt ttcactcata
      541 tattttctgc caccaccgag aattgaactc ggaattgagc attaccattg ctctattata
      601 ccatttaact atagcggcta cttttctatg atactataag attttgacga aatcaagtat
      661 ttctttactt agaaaaatag tcgttttttt agtctaattc tatttggaga aatttactat
      721 tttcaatagc tcttaaatgg tgtgtttctt gtggagagag gctaataagg ctatcttttt
      781 caaggatatg tttctgatca ccaaggataa tttcaagttt tctttccaaa acatttatta
      841 attttggatg agggcatcgt tcatttttca gtccccggct gatgatttta atggtattga
      901 ataaaccttc atctttaatc attcctaacg gggacataag acttattttt ccaaattgat
      961 attctgaact ttaaaaacta ttttgtaaga ataaattttg ccaatcatct atttttagag
     1021 gagcaacgtt aacattcata gtatgtctaa gttgatcgat cagcttggca gtgtcttcat
     1081 cttgataaga aacatcatgt gtgagtaaac gtccaccagg ttttaggaca cgtaagtact
     1141 cttttatggc tttctcctta gctgaattgt taagcatcgt taacattgct tcgttgataa
     1201 cgataaacta ttatcaggaa agggcagtct gagtgcattt ccctgaataa cttcaataaa
     1261 ttcttctaat tgggcctttt tgacattttc tttggccttt tctattactt tactatctag
     1321 atcaagcgct gtaatgtgac aggaaaatgc tttagcgata tcgatggaac tggtacattg
     1381 attacaagct acttctagaa cacgactgtc agcactaaag tgtccttttt tgataagcca
     1441 attcgttgct tggacacctc ctggtcttaa gcgttttttc ctagtcgtgc taagaattga
     1501 tgtccaattt ctttatgtgg caataagatc acctctttct tttaatagta atatcatttt
     1561 ttcttaatta taacacatag atgctaagat aacaaggagt aatagtgatt gaatggtata
     1621 agtgatgaat tttagagagc gttttctttt aactaggggt gtgatgccaa aactagaatg
     1681 gcatagagaa ccatacatct caaaaggtct tccttagata aaactagttg tttttatatc
     1741 tgtcagaaaa atcataattt atttttgata taatgatttt ttgagtattt aatttatatg
     1801 ttataatgaa aaaaactaaa aaagaggaat atcatgtcat acgaacaaga atttttaaaa
     1861 gactttgaag agtggttaca atcgcaaata agtatcaacc aaatggcaat ggacagtgca
     1921 aagaaagttt tagaggaaga taaggatgag cgagcagcag atgcgtatat tcgttatgag
     1981 agtaagttag acgcttatcg ctttttgcaa gggaaattta ataactatca taaccaaaaa
     2041 tctttccatg atttgcctga tggtcttttt ggacaacgtc attattaaaa ggtttatcgc
     2101 tagaaagctt gtcaaatagg catttctatg atacattgtt atagtagttt ttagatacta
     2161 tgatagattt cataaatgag gagaaagcat tatacaatgg caaagcgtat taaaccgaat
     2221 aaaaaaagag agcttttgaa agctaaaggg atcaattgga aggatgttcg actatctcaa
     2281 actttacaag aagtcgcatc accgaaaaaa aaggctcctg aagcgccaaa ggcagtcgat
     2341 cttttagaag gtttaactgc taataaacaa gatgttttaa aagaagctgg ccttgtcagc
     2401 cttgaagcgt ttgcgaaagt aagtgaagca gatgttttag cactaaaagg tattggtcca
     2461 gcagctatta aacagttagt tgataatggt gctgtttttg caaaatgatt tgattcacct
     2521 tgcgaaagta gtcttatttt gctagaataa attaacttag aggtgttttg aaatcccatt
     2581 tgtcacagga agcggtcata ctgagaaatc cgcacaaatg tcaaagcttg ttgctaagtc
     2641 caataattga aacaaaacga gttgcggatg ttatcatccg aatttgggtg gtaccgcgga
     2701 aatcgtaata cgaaattcgt ccctgtcaga attgacaggg attttttgta tttaggagtg
     2761 aggagattga agtatatgca gattctagaa gatttcgatg gcaaagcatt accaaaacta
     2821 gaaacggatc gacttatctt gcgtcagcga acggtggggg atgtcccagc catgtttgat
     2881 tacgtttgct tggaagaggt tgcctatcca gcagggctca gtccaatagc ttctttagaa
     2941 gatgagtatg attattttga aaatcgttac tatcaaaatc tggaaaaggc aaaattacca
     3001 tctggttatg gtattaccgt taaaggaagt gaccggatta tcggctcttg cgactttaac
     3061 caccgccatg aagacgatgt tttcgagatt ggctacatgc ttcatcctga ctattggggt
     3121 catggttata tgacagaagc tgtcgcagcc ttgattgaag tcggttttac cctcctcaat
     3181 ctccacaaaa tcgaaatccg ctgctatgat tacaataagc aaagccggcg cgtggcagaa
     3241 aaactaggct tcacccttga agccaccatc cgtgatcgta aggataatca aggtaatcgc
     3301 tgtgtcaatt tgatatatgg tttgctgagg agtgagtggg agtaattatt ctactcttca
     3361 tagaagttac tagatagact aatttggctg tgcagggtac ttagctaact catcaataat
     3421 atcgtagata tggaggagac aaatgattaa gatacttgga gaaatcgtag ataaccagct
     3481 acctgttgtg gaaacgaatc gtctgttgtt gcgtcaacgc aagttagaag atgctaagga
     3541 gatatttgaa tttgtcaaac ttgatgaggt gacttatcca gcaggttttc cggctgttaa
     3601 aagcttggaa gaagagataa cctatattca agagatttat ccaactaatc ttgaaaaaga
     3661 aaagttaccc tctggttatg ctattactct aaaaggcgat gataaggtaa ttggttcagt
     3721 agattttaac catcgccacg aagacgacat ttttgaaatt ggctacctgc tccaccctga
     3781 ttattgggga caaggcattg tgccagaagc agcttcagtc cttgtggaga taggtttcac
     3841 actgctagga ctacataaga ttgagctggg atgctatgac tataataagc aaagtcaggc
     3901 ggttgcgcgc aaattaggtt ttactttaga ggctaatatt cgtgatcgaa gagatgccca
     3961 ggggcaacgt tgtggcgata tgcgctttgg tttactgcga agcgagtggg agaaaaaaag
     4021 aagatgaata ttattatcat cggagcacaa gcttctggga aaatgacaat tggtcaggaa
     4081 atcgcaaaac agacaggcat gaccttattc cttaaccatg attctataga ttttgtttta
     4141 cgcttcatgc catggtctcc agatagtatt gctctaacgg agtctattcg ctttaaattt
     4201 tttgaaacgt ttgctaagac tggtcaagaa atgattttta ctattgtaat tgatttcaat
     4261 gattcaagag atgtagcatt tctagaaaag attcaaacag tttttcaatc tcacaaccaa
     4321 gaggtattat ttgtagaatt ggaaacagag ctaagtgagc gtctcaaacg aaatcgcaca
     4381 gaaaatcacc tcaagcataa gccttcaaaa cgtgatatca aatggtcaga gaacgatatt
     4441 tgttcaacca tggattacgc tatctttaat cctgaagtag ccccagaagc gcttacatat
     4501 tattataaaa tcaataatac ttgcttaact gctactgaaa cagcatatct tattattcaa
     4561 aaaataaatc aaatcaagga gaaaaactaa tgtcaaaaga actatcacca aaatacaatc
     4621 ctgccgaggt tgaggcaggc cgttatcaaa cttggcttga ccaagatgtt ttcaagccat
     4681 caggggatgc tgaggctaag ccttattcta ttgtgattcc accaccaaac gttacgggta
     4741 aattgcactt gggtcacgct tgggatacaa ctctacaaga tatcattatt cgtcaaaagc
     4801 gtatgcaggg ctttgataca ctctggcttc caggtatgga ccacgctggt atagccactc
     4861 aagctaaagt tgaggagcgc cttcgtgagc aaggcatttc acgttatgat cttggtcgtg
     4921 aaaaattcct agataaagtt tgggaatgga aagacgagta tgcagcaact attaaatctc
     4981 agtgggggaa aatgggtctt tcggttgatt atagccgtga gcgttttacc cttgatgagg
     5041 gactttcaaa agcggtaaga aaagtctttg ttgatttgta caataaaggt tggatctatc
     5101 gtggggagtt catcattaac tgggatccag cagctcgtac agccctttca gatattgaag
     5161 ttattcacaa ggatgttgag ggtgctttct atcatatgaa ttatatgctg gaagatggct
     5221 cacgcgccct agaagtagca acaactcgtc ctgagactat gtttggagat gtggctgtag
     5281 cggtaaatcc agaagatcca cgttacaagg atttgattgg tcaaaatgtt atccttccta
     5341 tcatcaataa acctattcca atcgtcgctg atgaacacgc tgatccagaa tttggaacag
     5401 gtgtggttaa gattacgccg gctcacgacc ctaatgactt cgctgtaggt caacgccaca
     5461 acttaccaca agttaacgtt atgaacgatg atgggactat gaatgaactt gcggatgaat
     5521 ttaatggtat ggatcgtttc gaagctcgta aggcagtagt cgctaaatta gaatcgttag
     5581 gaaacttagt aaaaattgaa aaaatgactc attctgttgg tcattcagaa cgaacaggcg
     5641 ttgtggttga accacgttta tctactcaat ggttcgtcaa gatggatcaa ttggctaaga
     5701 atgccattgc caaccaagat acagaagaca aggtagagtt ttatccacca cgatttaacg
     5761 atacttttat gagctggatg gaaaatgtcc atgactgggt tatctctcgt caattatggt
     5821 ggggacacca aattcctgca tggtacaacg tcaatggtga gatgtatgtt ggagaagatg
     5881 ccccagaggg tgacggatgg acacaggatg aagacgttct agacacatgg tttagttctg
     5941 ctttatggcc gttttcaaca atgggatggc cagacaccga agcagctgac ttcaaacgct
     6001 acttcccaac gtcaaccttg gtaactggat acgatatcat cttcttctgg gtatcgcgca
     6061 tgattttcca atctcttgaa tttacaggac gccaaccatt ctcaaatgtt cttattcatg
     6121 gactcatccg tgatgaagaa ggacgtaaaa tgtctaaatc acttggcaat gggattgatc
     6181 cgatggatgt cattgagaaa tatggtgcgg atgctcttcg ttggttcctc tcaaatggtt
     6241 cagcccctgg tcaagatgtg cgtttttcat acgaaaaaat ggatgcttcg tggaatttca
     6301 ttaataagat ttggaacatt tcacgctata tccttatgaa taacgaaggt ttaacgttgg
     6361 atcaagccag agaaaatgtg gaaaaagttg ttaatagtca agttggaaat gtaacagacc
     6421 gttggattct ccacaattta aatgagactg tgggtaaagt tactgaaaac tttgacaagt
     6481 ttgaatttgg tgttgctggt cacatccttt acaactttat ctgggaagag tttgctaatt
     6541 ggtacgttga gttaacaaaa gaggttcttt atagtgacaa tgaagatgag aaagttgtta
     6601 cacgttctgt tcttctttat accttagatc aaatcttgcg tcttcttcat ccaatcatgc
     6661 cattcgtgac agaagaaatc tttggtcagt atgcagatag ctcaatcgta acagctgctt
     6721 accctacggt taacccagcc tttgaaaatg ctgctgccca caagggagtt gaaagcttga
     6781 aggacttgat tcgttccgtg cgtaatagcc gagcagaggt caatgttgct ccatctaaac
     6841 caatcactat cttggttaag acaagtgaca gcgaactcga aagcttcttc aaagacaata
     6901 gtaactacat caaacgtttc acaaacccag aaacacttga aatcagctca gctattgcca
     6961 caccagaact tgccatgtca tcagtcatca caggagctga aatcttcctc ccacttgctg
     7021 acctcctcaa cgtagaagaa gagttagcac gacttgaaaa agaactagcc aaatggcaga
     7081 aagaactgga catggtaggc aaaaaactcg gcaacgaacg cttcctcgcc aacgccaaac
     7141 cagaagttgt ccaaaaagaa aaagacaaac aagccgacta ccaagctaaa tacgacgcaa
     7201 cccaagaacg gattgtagag atgcaaaaac ttgttaaata gttaaaatca tccattactt
     7261 ccgcagagtc ctgccgctag accatcagtc taagcgacag ggctttttgc tatattcaaa
     7321 gattgtgaaa aattaatata tatgatgcct acttcttgcg tctgaaatac aagaagtgaa
     7381 aatgatttgg ccataaagaa aaacaggcag aaacccaaat gattaatgcc tatttcttat
     7441 attttttcgt caattatttt ataaataaaa ttgaggtaca aaccagtttt taaaatgata
     7501 aggtgaatct tcttcaatat agatgtctgt atataaaaag gtatagctat ccatatcatc
     7561 aaaaatatca aaaggccact catcgttata atctaataat tccttaagtt gtagatggga
     7621 gttttcgtcc agagattcaa aataaagaat ggattctttt gcaattaaat agagataaat
//
LOCUS       DATRME010000088         7620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRME010000088 DATRME010000000
VERSION     DATRME010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(96..1292)
                     /locus_tag="VBC23_001837"
     CDS             complement(96..1292)
                     /locus_tag="VBC23_001837"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6843762.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQNPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1477..2412
                     /locus_tag="VBC23_001838"
     CDS             1477..2412
                     /locus_tag="VBC23_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6843763.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2393..2587)
                     /locus_tag="VBC23_001839"
     CDS             complement(2393..2587)
                     /locus_tag="VBC23_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6843764.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2676..4340)
                     /gene="dnaX"
                     /locus_tag="VBC23_001840"
     CDS             complement(2676..4340)
                     /gene="dnaX"
                     /locus_tag="VBC23_001840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6843765.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4340..4837)
                     /locus_tag="VBC23_001841"
     CDS             complement(4340..4837)
                     /locus_tag="VBC23_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6843766.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4924..5553)
                     /gene="udk"
                     /locus_tag="VBC23_001842"
     CDS             complement(4924..5553)
                     /gene="udk"
                     /locus_tag="VBC23_001842"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6843767.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            5698..6780
                     /locus_tag="VBC23_001843"
     CDS             5698..6780
                     /locus_tag="VBC23_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6843768.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(6813..>7620)
                     /locus_tag="VBC23_001844"
     CDS             complement(6813..>7620)
                     /locus_tag="VBC23_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6843769.1"
                     /translation="LLAHNISGFKAILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVK
                     DGKLIINDLKLPLDKLYNVIDESYLKSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPK
                     ALEILKRYNAKATFFIMGQSAVGHTDILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKE
                     EIHKTNDLIMKATGQKPVYLRPPYGATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMM
                     TNIKKQLRPGGVILMHDIHQTSIDALPTIMDYLTTQGYYFVTVGELYSTP"
BASE COUNT         2493 a         1447 c         1205 g         2475 t
ORIGIN      
        1 atttcctata cttaaccagt ttattataag attttataaa tgttctataa attatcatga
       61 gcaaaaaaag cgtaaacttt cgtttacgct ttttattatt tgctagcaat aaaatccttt
      121 aaggcctgga ctttatcaac tcgctcccat ggcaaatcga tatcagtacg tcccatatgc
      181 ccataggctg ctgtttgtcg ataaataggt cgtttgaggt ccaacatatt aataatgcct
      241 gctggtcgca aatcaaatat ttggcgtaca gctgcctcta ggtctgcttc tgctataaca
      301 cctgttccaa atgtatctac acgcacagag acaggctgag caacaccaat tgcataagca
      361 agttgtactt ctactttttt agctaaatca gctgcaacaa tattttttgc aatataacga
      421 gctgcataag atgctgaacg atctacttta gtagcatctt tacctgaaaa tgcaccacca
      481 ccatgacgag aataaccacc ataagtatct acaataatct tacgacctgt tagtcctgaa
      541 tctccttgag ggcctccaat aacaaagcgc cctgtaggat tgataaagaa ttttgtttgg
      601 tcatctaaat aatggcttgg aataacttca ttgataactt tctcaatcac gtccttatgg
      661 agttggtcat tagttacatt aggatcatgc tgtgtcgaaa taactactgc atccacacga
      721 attggttgat cattatcatc atattcaacc gttacttgag attttgcatc tggtcgaaga
      781 taggtgagtt ccccagactt acgtagatct gtcaatttct taactaactg atgagctaaa
      841 gaaattggca atggcattaa ctcaggtgtt tcatcaacag caaacccaaa catcaaacct
      901 tggtcacctg caccaattaa atctaaagga ttttgttcaa gagaaccacg tacctcaaga
      961 gcttcattga ctccctgtgc aatgtctggc gactgttcaa cgagtgacgg atgcactccg
     1021 acagattcag ctgagaagcc atattcagct ttatcatagc caatttcagc gattgtgtta
     1081 cggacaactc ggttaatatc aacataggct gtcgttgata tctcgccaaa tacatgaacc
     1141 gaacctgtat aaacagctgt ttcggctgct acgtgggcat ctggatcttg ctctaaaatt
     1201 gcatctaaaa tcgcatcgga aatttggtca gcaatcttat cgggatgtcc ctcagatact
     1261 gactcagacg tgaaaagttt acgttctgac ataaaaatgt cccccctaaa atgtaaaatg
     1321 tgtcgagtta caaagaacta gcctctaggc taccttttcg ggactcatat agctattgta
     1381 ccactatttc atcttttgtg aaataagtta ccaataaata aaaaattatc tctattttct
     1441 ttagtctttt ttctcgattt atactatact agatttatga aaacctacga aaaaatttat
     1501 caaatactat caaaaaataa caactttatt agcggagaaa ctatggcaaa tcaacttaat
     1561 atttcccgca cagctatttg gaaaggtata aaaacgttag aagaattagg cctagagatt
     1621 gagtctgtca ctaacaaggg ctaccgattg gtatcaggtg acatattatt accagaacaa
     1681 ctcgaacaag aaattggtat taaggttagt cttaataata atagtgcttc aacccaacta
     1741 gatgctaaaa tggggataga aagcaaatta aagactccac accttttttt agctcctaat
     1801 caaaaaaaag ctaaaggacg ttttgataga cctttcttta catcaaatca aggcggcatc
     1861 tatatgtctc ttttattaca acctaatgta ccgattgaag atataaaacc ttatactgtc
     1921 atggtagcct ctagtgctgt aaaagctatc tcacggttaa caggaattac tcctgaaatt
     1981 aagtgggtca atgatattta tttagataat aaaaaaatag ctggtatttt aaccgaggct
     2041 atcgcttctg ttgagagtgg attggtaacg aatgttatta taggattagg tatcaatttc
     2101 tatatcaaag agttccctag ggcgttaact aagcgcgcag gtagtctctt tacagagcaa
     2161 ccgaccataa ctagaaatca attgattaca gaaatctgga atctcttttt caatattcca
     2221 ctagaggatc acttaaaggt atatagagaa aaatccctag tcttagataa aaccgttagc
     2281 tttatggatg gtcaaacaat gtattctggg aaagctattg atattactga taaaggatat
     2341 ttagtggttg agttagatga tggtcagttg aaaacacttc gcagtggcga aatcagtctt
     2401 tcttcttggt agatttgata agctcttttg tgaattttga aatcgcatac accttttgaa
     2461 gatctcgaag taataaaact ccccaaaaaa tagcaaaaat ccaggagcca tccataacgg
     2521 attgattgaa aaaataagtt tcaaaagagc ataaaagcgc cataaaaata aattgtcgtt
     2581 ttgtcatatc accattataa cagaatctac taaataatta aaaaacttat caaaaaaata
     2641 gttgcctgtt acgggcaact atttttagtt acgttttaat cttcaacgat tgctattcgt
     2701 tctgctagat aagaaaagtc ttctggaatc tgatgattaa cttcaggttc tgtttccgtc
     2761 ttttgagctt tcattttctt tgcaaaatcg ctccgaattt gattaaaatc atttctaggt
     2821 acagctaaaa tattaggtga aaaacctgct gctttactca taatattacc aaatatatca
     2881 ttgagatcgg tacgtttcat agcttgttca gcattaaagg ccgcatcaaa tgccaaaata
     2941 gcattctcgc tattagctaa gacaggttca gatcccatta ataaggctct atcttgagcc
     3001 gtaatattat ctaatatctc gttccaagca ctctttaaag cttctaaata ttcacgtgaa
     3061 cgttggctat ctacaaccgt ttcttccata atggtcaaaa tctttgtacg gtcaactttg
     3121 aatgtgaatg tcttattatt aacctttact ttttgagttg atgaactttg atctgctcga
     3181 gagagttggc tcatttcgtt ttttaacgat tttaactctc gacgaagact atctaattct
     3241 gctgtcacat ctgcgggtat gtttgacgaa tttttttcta ccatttcaga caattgaatg
     3301 gtcatcatct ctgcataaac tttaggatgg ctaccatttt ttatctcagg aagaacagat
     3361 gtcactttat caatcatttc aaataaaata tcttgtttaa catcaagatt ggccataaaa
     3421 gtatcacttg aatgactatt ttcaccacct gcttgaacaa caagtaaatc tcttaagtac
     3481 atcaataaat ctgttgcaaa acgactcata cttttgccac tatcaaagat tacttcaagt
     3541 ttagcgagtg cttcagttgt atcgtgagcc aatatattcg atacataatc atctatagca
     3601 gataaggaaa tgctccctgt aatttcttct gctacatcta aagaaatgtg gttatctttc
     3661 gctaaactca aggcttgatc gagaattgat aaagcatcac gcataccacc ttcggctcgt
     3721 ctagcgacta gtgtcagagc atccaaatca tatgaaatag cttctttatc taaaatctgt
     3781 gctaaatgat ctcgaatagc taataattta atcgctttaa attcaaagcg ctggacacga
     3841 gataagattg ttgcagggat tttatggagt tctgttgttg ctaatataaa aacaacattt
     3901 tctgttggct cttcaagtgt tttcaatagt gcattgaagg cacctgtaga caacatatga
     3961 acttcatcaa taatataaac tttataggtt gccctacttg gagcataagt tgatttgtcc
     4021 ctgatatcac gaatttcatc gacaccatta tttgacgcag catcaatttc aatgacatcc
     4081 tctaaactac catttgtaat atctcgacaa atatcacaat gattacaagg ttcgccatta
     4141 acttgatttg ggcaattcat cgcttttgca aatattttag cagcacttgt ttttccagtt
     4201 cccctaggtc ctgaaaaaag gtaagcatgg ctaatttttt tgctggacac agcttgcttt
     4261 aaagtagttg aaataacact ttgtccaacc atttcatcaa atgtttggct acgatatttc
     4321 cgataaaggg cctgatacat tacttttcaa ctccaaacat atccaaattc caaatcgtat
     4381 gttctactag aatacctaca aatttttcta agtattcttg atcaatctca tcataatctg
     4441 ctactaaaga agaatctaaa tctagaactc ctagaagttt gccattttta aacatgggta
     4501 ctacgatttc actcatagct tttgaatcac aggagatata gttagcatgc tttgtaacat
     4561 catcaacaat cagcgtctta gcagtttgtg cagattcacc acaaacacct tttcctaaag
     4621 taatatgcac acatgataca ccaccctgaa aagggccaag aattaactcc tctccatcaa
     4681 ataaataaaa gcctgtaaat acagaatttg gaagcatagc atttagcata gctgaagcat
     4741 ttgataagtt ggcaagagca tttgtttcat ctgagaatag agcttgcgct tgtagtaata
     4801 ataattgata attttcgatt ttcttagact tgttcatagc acttattata gcataatttg
     4861 aaagttacta aaactaaata accatcctat ataaaacaaa aaaataaaca caaaccattg
     4921 tgtttatttt gcttcattta agatgctagc cactttggta ttgattaaat caatagcgac
     4981 aatattactt actccttctg gaattacaat atccgcataa cgctttgttg gttcgataaa
     5041 ttgatgatac ataggtttta caacttctgt atactgttca ataatactat ctaaacttct
     5101 atcgcgctct tccatatcac gcttaatacg acgaataata cgaatatcat catctgtatc
     5161 aacaaataat ttgatatcca ttaaatcacg caaacgctga tcttctaata ccaaaattcc
     5221 ctcaacaata atgacatcct gaggttcttg gcggatagtt ctatcactac gggtatgttt
     5281 tgtgtaatca taaaccggaa tatcgaccgg acgcccctca atcaattcgt tcaattgctc
     5341 aatcatcaaa ttggtatcaa aagcaagggg atgatcataa ttggttttta cacgttcttc
     5401 aaaagtcaaa tgactttgat ctttatagta agaatcatgt tcaatcatga taattttttg
     5461 atctgggaaa tttgataaaa tcgcccgaga aacactggtt ttaccaccac cagatccacc
     5521 tgtaacacca ataataatag gttttttacg catttgtcac tccaaattaa atttatcctc
     5581 tatctattgt actataaact tatttttttt aaagtctaaa ttttattgaa aacgctctaa
     5641 aaataaaaag catgttataa taacaatgac aacatataaa ataacaagag gattcttatg
     5701 ataacaaaat ttccagacca atggcaggat aaattaaccc agcgtcaatt tgatgatcta
     5761 acagatattc aaaataaact ttttcaacct attactgatg gtgataatat ccttggaatt
     5821 agccctacag gtactggtaa aactctagca taccttttcc caacgcttct taagttgcaa
     5881 cctaaaaaga gccaacaact cttaatttta gctccaaatt cagaattagc tggacaaatc
     5941 ttcgacgtta caaaagaatg ggctgaacca ttaggactga cagcacaact attcctttca
     6001 gggtctagtc aaaaacgcca aattgagcga ctaaaaaaag gaccagaaat ccttatagga
     6061 actgctggac gcgtatttga acttgtgaaa ctaaaaaaaa taaaaatgat gaacattaat
     6121 actatcgtcc ttgatgagtt cgatgaatta ctcggcgact cacaatatca ttttgtggac
     6181 aatattatta atcgcgttcc tcgtgatcag caaatgattt acattagtgc caccaacaaa
     6241 ctggacaata gtaaacttgc cgataacact ataacaattg acctatctga tcaaaaactt
     6301 gatactataa aacattatta tattacagtt gataaaagag agcgtactga cttacttcgt
     6361 aaattttcaa atattcctga ttttagaggt cttgtctttt ttaacagctt atcagactta
     6421 ggtgcatgtg aagaacgttt acaatttaat agggcctcgg cagtttcttt agcaagcgat
     6481 attaatatta aattccgtaa agttatctta gagaaattta aaaaccatga tatttcatta
     6541 ctcctaggaa ctgatttggt agctcgtggc attgatatcg acaacctaga atatgtgatt
     6601 aattttgata tcgcacgcga caaagaaaca tatacacatc gttctggacg aactggtcgt
     6661 atggggaaag agggatgtgt tattacattt gtaacacata aagaagagct caaacaactt
     6721 aaaaaatacg ccaccgttgc agaacttgta cttcacaatc aaaaattaca cttaaaataa
     6781 aaaaagaaac aagtaacttg tttctttttt tattaaggcg ttgaatataa ttcacctaca
     6841 gtaacaaaat aataaccctg ggttgtcaga taatccataa ttgtaggcaa agcatctatg
     6901 gatgtctggt gaatatcatg catcaaaatc actcccccag gacgtaattg ttttttgata
     6961 ttggtcatca ttgcttgtgt attatgattt ttccagtctt ctgtatcaac tgaccacaac
     7021 atttctttca atcctgtcac tgtttttaca gtagcatttg ttgctccgta agggggacgt
     7081 aagtacactg gtttttgtcc tgttgctttc atgattagat cattagtctt atgaatctct
     7141 tccttgattt tctccgctgg tagtttggtt aaattaggat gatcccatgt atgattacca
     7201 atctcatgac cttcagcatg catcctttgt aaaatatctg tgtgccctac agcactttgt
     7261 cccattataa agaaagtcgc tttagcatta tatcttttaa ggatttctaa ggctttagga
     7321 gtcgtttttt catttggtcc atcatcaaag gtaagtgcaa cacgtggtgc tttagctttt
     7381 aagttagaaa ctaaatctag atcacttgat tttaaatatg actcatcgat cacattatat
     7441 aatttatcaa gtggtaattt taaatcatta ataattaact taccatcttt aacatctagc
     7501 ccatctgtta ctcttccagt ttttaaaaat tctttattgc tatcatgttc ctttagttga
     7561 gttcctggag ctatattttc taaaatagcc ttaaatccag atatattatg agctaaaagc
//
LOCUS       DATRME010000089         7384 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRME010000089 DATRME010000000
VERSION     DATRME010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            17..1387
                     /locus_tag="VBC23_001845"
     CDS             17..1387
                     /locus_tag="VBC23_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6843770.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(1508..2497)
                     /locus_tag="VBC23_001846"
     CDS             complement(1508..2497)
                     /locus_tag="VBC23_001846"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6843771.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            2736..5195
                     /gene="gyrA"
                     /locus_tag="VBC23_001847"
     CDS             2736..5195
                     /gene="gyrA"
                     /locus_tag="VBC23_001847"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6843772.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            5202..5945
                     /locus_tag="VBC23_001848"
     CDS             5202..5945
                     /locus_tag="VBC23_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6843773.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            5961..6374
                     /locus_tag="VBC23_001849"
     CDS             5961..6374
                     /locus_tag="VBC23_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6843774.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            6527..>7384
                     /locus_tag="VBC23_001850"
     CDS             6527..>7384
                     /locus_tag="VBC23_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6843775.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKN"
BASE COUNT         2449 a         1175 c         1504 g         2256 t
ORIGIN      
        1 aaggagaact cttaatatga gtaaaatcgt tgtcgtaggg acaaaccacg ctggtacagc
       61 agcaattaaa acaatgctta gtaattatgg tgaagccaat gaaattgtaa catttgatca
      121 aaattccaat atctctttct taggatgtgg tatggctctt tggattggtg aacagattga
      181 tggtccagaa ggattgttct actctgataa agagcagttg gaaagtatgg gagctaaggt
      241 ttacatgaac tctccagtac ttaatattga ctacgataaa aaagaagtta cagctttagt
      301 agatggaaaa gaacatgttg aaagttatga aaaacttatt ttagcaactg gttcacaacc
      361 tatcattcca ccaattaaag gtgttgaaat tcaagaagga tctcgtgaat ttaaagctac
      421 tcttgaaaac cttcaatttg tgaaacttta tcaaaattca gaagaagtta ttgaaaagtt
      481 agctaaacct ggtatcaatc gtgttgctgt tgttggggct gggtatattg gtgtagaatt
      541 agcagaagct ttccaacgta ttggaaaaga agtaacactt gttgatgttg ctgatacttg
      601 tatgggtggt tactatgatc gtgactttac tgatatgatg agtaaaaacc ttgaagacca
      661 tggtattcgt cttgctttcg gacaagctgt tcaagcagtt gaaggtgatg gtaaagtaga
      721 acgtcttgtt actgataaag aaacatttga tgttgatatg gtgatcttag ctgttggatt
      781 ccgtccaaat acagaactcg gtgcaggtaa attagataca ttccgtaatg gcgcttgggt
      841 agttgataag aagcaagaaa caagcgtgaa agatgtttat gctataggtg attgtgcgac
      901 aatttgggat aattctcgtg atgatatcaa ttatattgct cttgcttcaa acgccgttcg
      961 tactggtatt gtagcagctc acaatgcatg tggtacagag cttgaaggtg ctggtgtcca
     1021 agggtcaaat ggtatctcaa tctatggcct taacatggtg tcaactggtt tgactttaga
     1081 aaaagcaaaa caagctgggt acaatgcagt tgaaacaggc tttaatgacc ttcaaaaacc
     1141 agaatttatc aaacacaata atcatgaagt agctattaag attgtctatg ataaagatag
     1201 ccgagttatc cttggttgcc aaatggtctc acacgaagat gtatctatgg gaattcatat
     1261 gttctcactt gctattcaag aaaaagttac tattgaaaag cttgctttga ccgatatctt
     1321 cttcttacca cattttaata agccatataa ctatattaca atggctgcat taggcgctaa
     1381 agattaattt aaaattaaaa aggttaagcg aaatgcttag ccttttattt catcctttaa
     1441 caaggtcaaa aatgtaacgg caaagaaaaa gctaagatat tcctatccta gctttttttc
     1501 aactatgtta gttttttgat gcttcttgga attctgggtt tttccaagct tcgtcaataa
     1561 tatcttttaa ttgttcagct gaggcttgca ttttttgaag ttcagcatca tttaatggga
     1621 tattaactgg acgaacgata ccatgtgcac ctacgattgc aggctgaccg ataaagacat
     1681 ctttaacatc accgtattgg ccttcttgat atacagataa tggaagaact gcattttcat
     1741 catcaaggat agctttagta atacgagcaa gtgcgacagc aataccgtaa tatgtagcac
     1801 cttttttgtt gatgattgag tatgcagcgt cacgaactga aataaacaaa tcaacaagtc
     1861 cttgttcatc aatatcgcga ttttcttgta accattgttc aagttggaca ccagcaacgt
     1921 tagcatgtga ccaaacggca aattctgagt caccgtgttc acccatgatg tatgcgtgaa
     1981 ctgaacgagc atcaacacca attttatctg ctaaagcttg acggaaacgt gcagagtcaa
     2041 gtgaagtacc tgaaccaata acgcgttcct tagggaaacc tgagaatttc caagttgaat
     2101 atgtaagtac atctacaggg ttagccgcta ctaagaagat accgttgaaa cctgattcaa
     2161 caacttgagt tacgattgat ttgttaattg ctaagttttt accaacaagg tcaagtcgag
     2221 tttcacctgg tttttgaggt gcgccagcag taatgacaac aaggtcagca tctgcgcagt
     2281 ctgcataagt agctgcgtag atttttttag gtgatgtaaa tgcaagggca tgtgaaagat
     2341 cttcagcatc accaacagct ttatcaaata aagctgggat ttcaatgata cctaactctt
     2401 gcgcaatacc ttggttaaca agtgcaaaag cgtaagaaga acctacggca ccatcaccaa
     2461 cgagaataac ttttttatgt tgtttagttg cagtcatttc taaacatctc cttaaatatt
     2521 tttgggaagt ttcccataca cctatattct atcattttca atgaaagaag tcactaataa
     2581 aatgtgaaaa aatttgcaaa caaattgaaa tatcatagaa aactattgaa aacgctgagt
     2641 gtcttgatat ttttgatacc gatttatcat cgcaatttta cctaaattat ggtacaatgt
     2701 aagagactta cagtgaagaa aaggggcatt ttttaatgca agataaaaat ttagtagatg
     2761 ttaatctaac tagtgaaatg aaaacgagtt ttatcgatta cgccatgagt gtcattgttg
     2821 ctcgtgcact tccagatgtt agagatggtt taaaacctgt tcatcgtcgt attttatatg
     2881 gtatgaatga actaggtgtg acacctgata aacctcataa gaaatcagca cgtattactg
     2941 gtgatgttat gggtaaatac catccacatg gtgattcatc tatttacgaa gcaatggtgc
     3001 gtatggcaca atggtggtca tatcgtcata tgctcgttga tggtcatgga aactttggtt
     3061 caatggatgg ggatggtgct gccgcacagc gttatacaga agcacgtatg agtaaaattg
     3121 ctcttgagat gcttcgtgat atcaataaaa atacagttga ttttcaagat aactatgatg
     3181 gcagtgaacg tgaacctctt gttttaccag cgcgttttcc taatttatta gtcaatggtg
     3241 caactggtat tgctgtaggt atggcaacaa atattccacc acataatcta ggtgagtcta
     3301 tagatgctgt caaattggtt atggataacc ctgatgttac tacacgggag ttaatggaag
     3361 ttattcctgg tccagatttt cctactggtg ccttggtgat gggacgttca ggtattcacc
     3421 gtgcctatga aactgggaag ggatcaattg tcttacgttc acggactgaa attgaaacaa
     3481 cttcaaatgg gaaagagcgt attgttgtca cagagtttcc atatggcgtt aataaaacaa
     3541 aagtacacga gcatattgta cgtttagcac aagaaaagcg cattgaaggt attactgctg
     3601 ttcgtgatga atcaagtcgt gaaggcgttc gttttgttat tgaagttcgt cgtgatgctt
     3661 cagcaaatgt gattttaaat aatcttttca aattaactag cttgcagaca aattttagct
     3721 tcaatatgct tgctattgaa aaaggtgttc caaaaatttt gtcattacga caaatcattg
     3781 ataactatat tgaacaccaa aaggaagtta ttgttcgtcg tactcagttt gacaaagcta
     3841 aagcagaagc ccgtgctcat atcttagaag gtttacttgt ggcgcttgat cacttggatg
     3901 aagttatcac aattattcgt aatagtgaga cagacaccat tgctcaagca gagttgatgt
     3961 cacgttttga attatcggag cgtcagagtc aagctatttt agatatgcgt cttcgtcgtt
     4021 taactggatt agaacgcgat aaaatccaat cagaatacaa tgacttacta gctttaattg
     4081 ctgatttagc agatatctta gctaagcctg aacgtgttgt taccattata aaagaagaga
     4141 tggatgaggt taagcgtaag tatgctgatg cccgtcgtac agaattaatg attggtgaag
     4201 ttttatcact tgaagatgaa gaccttattg aggaagaaga tgttcttatt actctctcaa
     4261 acaagggata tatcaagcgt ctcgctcaag atgagtttag agcacaaaaa cgtggaggac
     4321 gcggtattca agggactgga gttaataacg atgattttgt tcgtgaattg gtttcaacaa
     4381 gtactcacga tactgtgctt ttctttacaa atttagggcg tgtgtatcgt ttgaaagcat
     4441 atgaaattcc agaatatgga cgtacagcta aaggcctccc tatcgttaac cttttaaaac
     4501 ttgacgaagg tgagacaatt caaacaatta ttaacgctag aaaagaagat gtcgctaata
     4561 aatatttctt tttcacaact cagcaaggta tagtgaaacg tacaagtgtt tctgaattta
     4621 gtaacattcg tcaaaatgga cttcgtgcta ttaatttgaa agagaatgac gagttgatta
     4681 acgtcctctt gattgacgaa aatgaagatg ttattatcgg gacacgtaca ggctactctg
     4741 ttcgcttcaa agtgaacgct gtacgtaata tgggacgcac agcgactggg gtacgaggcg
     4801 ttaatcttcg tgaaggtgat aaagttgtgg gagcttctcg tatcgttaat ggacaagaag
     4861 tactaatcat cactgaaaaa ggttatggca agagaacaga agcttcggaa tatcctacaa
     4921 aaggccgtgg tggtaaagga ataaaaaccg ctaatattac tgctaaaaat gggccattag
     4981 cacgcttagt gacgattaat ggaaatgagg acatcatggt cattacagat acaggtgtta
     5041 ttattcgaac caatgttgct aatatttctc aaactggtcg ttctaccatg ggtgttaaag
     5101 tgatgagact tgatcaagaa gctaaaatcg tcactgttgc gctagtggaa caagaaattg
     5161 aagataagtc taatatagag gatacaaaag aataggaagt tatgcgtaat aaaaaaaaat
     5221 cacatggttt ctttaacttt gttagatggt tattggttgt attgttaatt attgttgggt
     5281 tggctttggt gttcaataaa ccaattcgca atgctttcat agctcatcaa tcaaatcatt
     5341 atcaaatttc acgagttagt aaaaaaacaa ttgaaaaaaa taagaaatct aaaacaagtt
     5401 atgacttttc atcagtcaaa tctattagta cagaatcgat tttatctgct caaacgaaat
     5461 cacataattt gcctgtaata ggagggattg ctataccaga tgtcgaaatc aatctcccaa
     5521 ttttcaaagg actgggcaat acagagttga gttatggtgc aggaacgatg aaagaaaacc
     5581 agattatggg aggcccaaat aactatgcct tagctagtca ccatgtcttt ggtttgacag
     5641 gttcttctaa gatgctcttt tcacctttag aacatgctaa gaaaggtatg aaagtttacc
     5701 tcactgataa aagcaaagta tacacttaca ctattactga aatttcaaag gtaacaccag
     5761 aacatgttga ggttattgat gatacacctg gcaagagcca actcacttta gtgacctgta
     5821 cggatccaga agccacagaa cgtattattg tgcatgctga attagaaaaa acaggtgaat
     5881 tttcaacagc agatgagtcg atattaaaag cattttcgaa aaaatataat caaattaatc
     5941 tctagaaggt agttagaatt atgaaattaa aggctgttca tcatattgcg ataattgttt
     6001 ctgactatga aaagtcaaaa gacttctatg ttaataagct aggatttgaa attattcgtg
     6061 aaaaccatcg tccggagcgt catgattata aattagattt acggtgcggt gatattgagc
     6121 ttgaaatatt tggaaatcgt cttgatgatc cagaatatga aactcctcct cagagaatag
     6181 gtaggccgaa ttggccacgt gaagcatgtg gtttacgaca ccttgccttt tacgttccag
     6241 atgtggaagc gtacaaggta gaactggaga atttaggaat ttttgtagag cctattcgtt
     6301 acgatgacta tactggcaaa aagatgacct ttttctttga tccagatggt ttacctttag
     6361 aattacatga ataataaaca aagttgcgtg gatacgcaac tttgtttaat ttactgaata
     6421 attttatatt caagaagttt tttaaagaaa taagtaagtt aatcatgata taatgtaagg
     6481 tatgaaaaga attgaaaagg aattattttg aaatgaggat actaaaatga gtattaaaaa
     6541 gctattgttt gcaggccttg cttttatttt atttacccta gcgagtccag catatgctgc
     6601 atcagatgtt cagaaagtta ttgatgaaac ttatgttcaa cctgattatg tcttaggtta
     6661 ttcgctaaat caagaacagc gtgcacagac attacaactt ctgaattatg atgaatctag
     6721 agacacgaaa gttaaaacgc taaatactag ctcatatgct aaaataatga atatagctga
     6781 tgatgcaagc atccaattat attcatctgt taaaataaaa aaattagggt ctaacgatac
     6841 cttagcagta aacattgtta cacctgagaa tattactaaa gttacagaag atatgtatcg
     6901 caatgctgca gttacattag gtattgagca tgcaacgata tcagtggctg ctccaataaa
     6961 agtaactggg gagagtgctc ttgcaggtat ctattattca ttagagaaaa atggagcaag
     7021 tgtatcatca gaaaataaac aactagcaca agaagagtta agtactttat ctggtattaa
     7081 tgctgagaat aagggtaagg aaggttatga cgccgacaag cttaatgtag ctttaacgga
     7141 tattaagtca gccgtagcta aaggaggatc tgatctttca aaagatgata ttcgaaaaat
     7201 tgttgaggaa acattgaaaa attatcatct tgataacgct gtaacggaaa atcaaataaa
     7261 tttaattgtt aattttgcag ttaatctttc acaaagtaat gtcatcaaaa atagtgattt
     7321 cacgaacacc ttgaataatt tgaaagacaa cattgtttcg aaagcaggat ctaaatttaa
     7381 aaat
//
LOCUS       DATRME010000090         7233 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRME010000090 DATRME010000000
VERSION     DATRME010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1634
                     /locus_tag="VBC23_001851"
     CDS             <1..1634
                     /locus_tag="VBC23_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO6843776.1"
                     /translation="KKHQYILLILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSS
                     VANTRVLTIGVSGLIGGPIVGIIVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSG
                     NYLRKNYNKIRVLDAMVVGFGMEILQMICILIFSVDFNQALRLVSFISMPMILSNTLG
                     LGIFISIISSTQKLEEHAKAFQTHQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMD
                     VSAVSLTSQSAILAYVGDGADHHLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRN
                     CPLSSAIVIPLHIHDVIVGTLKLYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEA
                     TRLLQDAEMKSLQAQVNPHFLFNALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAK
                     QNLIPLHDELEQVNAYLALEEARFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYK
                     HAFKHINKNNQLKVTIARNNDRLHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIE
                     NLVRRLNIIYDGQASLKFESNDSGTCAIVNIPIKN"
     gene            1646..2380
                     /gene="ltdR"
                     /locus_tag="VBC23_001852"
     CDS             1646..2380
                     /gene="ltdR"
                     /locus_tag="VBC23_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6843777.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2536..4020
                     /locus_tag="VBC23_001853"
     CDS             2536..4020
                     /locus_tag="VBC23_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6843778.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
     gene            4222..4794
                     /locus_tag="VBC23_001854"
     CDS             4222..4794
                     /locus_tag="VBC23_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6843779.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            5070..5921
                     /gene="ylqF"
                     /locus_tag="VBC23_001855"
     CDS             5070..5921
                     /gene="ylqF"
                     /locus_tag="VBC23_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6843780.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            5908..6669
                     /locus_tag="VBC23_001856"
     CDS             5908..6669
                     /locus_tag="VBC23_001856"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6843781.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            6690..>7233
                     /locus_tag="VBC23_001857"
     CDS             6690..>7233
                     /locus_tag="VBC23_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6843782.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKL"
BASE COUNT         2424 a         1233 c         1295 g         2281 t
ORIGIN      
        1 taaaaaaaca ccagtatatt ctgcttatct tatttagctt atttgctatt atttctaatt
       61 ttacaggggt agaaattcag tcagatttat ctatcattcc acaaactctt aatcatatag
      121 cagatcaatc atcagttgcc aacacacgtg ttttaaccat tggtgtatca ggtctaatcg
      181 gtggccccat agtcggtatt atagttggtc tcttatctgt attcgttagg tacttgcaag
      241 gaggattagc ccctcatatt tatgtcattt cttccttact aattggttta tgctctgggt
      301 tatcaggcaa ttatcttcgt aaaaattata ataaaattcg tgttttagat gcaatggttg
      361 ttggtttcgg gatggaaatt ctacaaatga tctgtatcct catttttagt gttgacttta
      421 atcaggcgct tcgattagta tcttttatca gcatgccaat gattttaagt aatactttag
      481 gtttaggtat atttatatcc attatttcat ctactcaaaa attagaagaa catgccaaag
      541 catttcaaac ccatcaagta cttgaattag caaatttaac ccttccttac cttagaaagg
      601 gactgacaac agaatcttgt caaccagtag ctgaaattat tcataaacat atggatgtga
      661 gtgctgttag cctgactagt caatcagcca ttttagccta tgtaggagat ggagctgacc
      721 accatcttcc taatactcaa atattaacaa agcttgctaa aagagcaata gatactggta
      781 aagttagtgt cgcaactgat aaaagtgaaa tcgaatgcga ccataggaat tgcccactta
      841 gttcagcaat tgttattcca ttacacatcc acgatgttat agtgggtact ttgaaattat
      901 atttttcaga cgcacaacat atgacttacg ttgaccgtca attagctgaa ggattaggca
      961 atatcttttc cactcagtta gctttgggac aagctgagga agcaacaagg ttactacagg
     1021 atgcggaaat gaagtctctc caagcgcagg ttaatccgca ttttcttttt aatgcactca
     1081 atactattta tggtttaatt cgcatggata gcgaaaaagc aagaaagctc gtccaagatt
     1141 ttagcaaggt tattcgtgct aatcttcaac gggcaaagca aaatttaatt cctctccatg
     1201 atgaattgga acaggttaac gcctatttag cccttgaaga agcaagattt cctaacatgg
     1261 tggcctttaa tttagacaac caaacaaatt cagatgacaa cttaatgatt cctcccttta
     1321 cattgcaggt cttgattgaa aactcttata aacacgcttt taagcatatc aataaaaata
     1381 accagctaaa ggttactatt gcaagaaata atgataggct tcatatcatt gtccaggaca
     1441 atggtattgg gattccaaaa gaaaaattaa taacgcttgg aaaaaaaact caaatttcaa
     1501 aaaaaggttc tggtacagct attgagaatc tcgttcgtcg cttgaatatt atttatgatg
     1561 gacaagcaag tttaaaattt gaatcaaatg attcaggaac ttgtgctatt gttaacatcc
     1621 ccattaaaaa ttagaaaggt attgtatgaa agttttagta gttgatgatg aaccagttgc
     1681 acgtaacgaa ttaatttacc ttcttaataa gtatgattct aacctcgtta tagcagaggc
     1741 gcatgatatg gctactgcat tagctatttt acttagagaa acttttgatg tagcactgtt
     1801 agatatccat ctcagagatg attctgggtt gcaattagca gagtatatca ataaaatgcc
     1861 caaaccacca ttattgatat tcgcgactgc ttatgatcaa tatgctattc aggcttttga
     1921 gcaggatgcg cgtgattatt tgttaaaacc ctatgagttt gataggttaa agcaagctat
     1981 ggatagagta aaaggagcgc taagtacatc tacaattata gagagcgtag cttccggtcc
     2041 tctcttcaag caacagtatc cattgacagt agaagatcga atctatctgg tgtcggcgga
     2101 tgatatcctt ttgattgaag ctatgcaagg aaaactgatt atacaaacac ctgataaaaa
     2161 ttatgaaatt gatggctctc tacaacaatg gcaagataaa ctaccatcat ctcaatttgt
     2221 acgggtacat cgctcttaca ttgtgaatat taatgctatt aaaacgattg aaccttggtt
     2281 taaccaaaca cttcagttac acctttgtaa taaaataaca gttcctgtta gcagagcaaa
     2341 tgtaaaaccc ctaaaacaaa tgttaggcat atctacctaa ctaatgaaaa gttattcatc
     2401 ataaaactta gactattcat ttctaaaaaa caaccattca tcttgaaaat aggaaatttc
     2461 aatttttcaa tgctatacta aagtcatgaa aacgcttaat aaaattaagc aaaaaaattt
     2521 ttaggaggca gtgatatggt aacattttta ggaggggtag cccttttaat tgttggttat
     2581 ttcacgtacg gtagatacat tgaaaagaat tttcaaattg atgaaaatcg ccaaacacct
     2641 gcagaagcat taagggatgg atatgatttt gttcctatgc caaaatggaa gaatgggatg
     2701 atcgagttat tgaacatcgc aggaactgga cctatctttg gacctatttt aggagcactc
     2761 tatggtccag ttgcttatat ctggatagtc ttaggatgta tttttgcagg ggctgttcat
     2821 gattatatga ttggaatgat ttctcttaga aataatggtg catatcttcc tgaattagct
     2881 agtcgctatc ttggaaaatc aatgaagcat gtgatcaaca ttttttcaat gctattactt
     2941 attttagttg caaccgtatt tgtggtaact ccagctaatc ttatcctgag tattttacca
     3001 gctgggacac tatctttacc ttggataatc ggtcttattt tcgtttatta ccttatttct
     3061 acagtattac ctattgataa agcacttggt aaagtttatc ctgtattctg cgtcatccta
     3121 atggttagta cagcagcagt tggcttccgt ttattaactg gtggctttga tatgccaaac
     3181 ttaacttttg aaacctttaa aaatatgcat ccagctggac ttgggatatt ccctgcttta
     3241 ttctttacca tttcatgtgg agccatctct ggtttccatg ctacccaagc acctatggtc
     3301 tcaagaacaa ctgttaatga gagagaaggg cgtttcactt tctatggtat gatgattgca
     3361 gaaggagtaa ttgcgatgat ttgggcaggt gcttcaatga gcttgttcaa aggtcaaaat
     3421 ctctatgaaa tgattgctgc tggaacacct tctgcagttg tcaatcaagt aatgttaatg
     3481 ttactcggta gtgtaattgg aactatcgct atcattggtg ttattgttct ccctgtttca
     3541 tctggtttat cagctttccg tagcttacgt actatcgttg ctgattatat ccatgtcaaa
     3601 caagatactc ttccaaaaat ctttgctgta actatcccat tatatgttat ttcatttgtc
     3661 cttacacacg ttgattttaa tctcctatgg cgttacttta actgggcaaa tcaagtaact
     3721 gctgttattg gtttattagt tgctacaaga tatctcatcc taaaaagacg aaattattgg
     3781 gtaacatttg taccagctat gttcatgttg tatgcagtag ttgtttatat tttatctcaa
     3841 ccaattggat ttaatatggg cttaggcatc ttaacttatt cacttgcttt agtgttaaca
     3901 ggtattcttg ttggactttt ctggaaatct ggtcaaaaac aattaaaaac tgttcatcct
     3961 gaagcattcc tgtttaacga tcatagacca attaattatt acagtagttt agattcttaa
     4021 tattattttg aagccatgtt attaagactt acagttatca caataacctc gaatgaagta
     4081 tcattcgagg ttatttttct actaaaaatc tcagtaaata tttctatttg attacaaatg
     4141 ttacaaaaat atttgacaac cttctttctg aaacttataa tagatttgtc cataacagag
     4201 caaaagagag agggatagta catgagaaaa cgttcaattt tgccaatatt agctgtctta
     4261 tttgttttca tgagtatctt tagcgtatac tctatttttc atggtgataa agctaaagaa
     4321 aaagcaagtg tcataaaaca agcatctcaa acaagtcaaa cttcaaaaaa agaggtctta
     4381 caaaagaaga cttatccaaa tctaaataaa tattcaaatc tagaaattca tgtttcatct
     4441 acgaggcaaa caatgacaat aacctcgagt gacaaggtca tctttaaaac gattgtttca
     4501 acaggagcaa aagagagccc taccccaaaa ggaacttttg tcatagaacc ggaacgagga
     4561 gattttttct ataacgcctc atcaaaagaa ggagcctatt attgggtctc ctttaaagag
     4621 catggtattt atctctttca tagcgttccg actgatcaac aaggaaatga gatcccagaa
     4681 gaagcaaagc aattaggaaa agccgcctca catggctgtg tgcgtatgtc aagggcggat
     4741 gcaaaatggt tctatgaaaa tattcctcaa ggaacgactg ttaccatcaa atagtcttaa
     4801 tgtggtaaaa atcatgttat taaaagcttg gagtaaactt ccagagcttt ttcgtatatc
     4861 ccttaaactc aattagtaaa cgaaacctat cagcggaata aatgtataaa gtatttcgat
     4921 atcattaaaa aaatgataga ataatagtgt taatcgaata ttttatcgac ctttatatgc
     4981 ggttgaagaa gaataaaact aaaagattag acctctaaca aatagatgta gacttttaag
     5041 gtagtctata cataaggaga atttatttca tggctactat tcaatggttc ccaggacaca
     5101 tgtccaaagc tcgccgtcaa gttcaagaaa atattaagca tgttgatttt gttacaattt
     5161 tagtggatgc aaggttacct ctttctagtc aaaatccaat gctaaccaaa atcgtcggag
     5221 acaaacccaa actaatgatc ctcaataaag ctgatctagc agatcccatt cgaactaaag
     5281 aatggagaga tttttatgaa tctcaaggtt taaagacatt agctattaat tctaaagaac
     5341 aatcaacagt taaaaaagta accgacatcg ctaaaatatt aatgtccgat aaaatcgcta
     5401 aactacggga acgtggtatt caaaaagaga cacttcgtac gatgattatt ggaattccga
     5461 atgcaggcaa atcgactcta atgaatcgcc tagcaggtaa aaaaattgca gtggttggta
     5521 ataaaccagg tgtcacaaaa ggtcagcaat ggctcaaatc taataaggaa ctggaaattt
     5581 tggacactcc tggtatcttg tggcccaaat ttgaagatga attagttgga ttaaagctag
     5641 cattaacagg ggcaatcaaa gatcaattat tgcctatgga tgaggtaaca atatttggat
     5701 tgaattactt taaaacctat tatcctgatc gtctcaaaga gcgttttaaa agtattaatt
     5761 tagaggatga atctcctgaa atcatcatga ccttaacaca aaaattaggt tatcgtgacg
     5821 attacgatcg cttttataat ttatttgtca aagaagttcg cgatggtaaa ttaggacgct
     5881 atacgttaga tatcgtcgga gaacatgatg gcaacaatta aagaaatcaa agcaatttta
     5941 gagactattg ttgatttaaa agacaaacgt tggcaagaat atcaaacaga ttcacgcgca
     6001 ggtgttcaaa aagcaatcct tcaacgaaaa aaaaatattc agtcacattt agacgaagaa
     6061 gcgcgcttag aacaaatgtt ggtctacgaa aaaaaacttt acattgaaca tataaacctt
     6121 attgcaggaa tcgatgaagt aggtagaggt cctttagcag gaccagtagt agctgctgct
     6181 gttattcttc caccaaactg taaaataaaa catctcaatg actccaaaaa aattccaaag
     6241 aaaaagcatc aagaaatcta tcaaaatatt ttagatcaag cgttagcagt tggtattggt
     6301 attcaagaca gccaatgtat tgatgatatc aacatttatg aagctacaaa acatgctatg
     6361 attgatgcag tttctcattt atctgtagtt cctgaacatc ttttaattga tgctatggtt
     6421 ttggatctac caattccaca aactaaaatt atcaaaggtg atgctaattc actctctatt
     6481 gcagcagctt caattgtagc taaagtgacc agagataaaa taatgtctga ttatgatagc
     6541 aaatatcctg gatttgcatt ttctaaaaat gctggctatg gtactaaaga gcatttagag
     6601 ggtttgcaaa aatacggtat tactccgata catcgtaaaa gttttgaacc tataaagtca
     6661 atgttgtaaa taatacggaa gaggaaaaga tgaccgaaaa agaaaaaatg ttagcaggac
     6721 aatattatcg tccatcagca cctgaactac gcaaggatag agaagttgca ctaaaaaata
     6781 tgcaagcctt taacaatgaa gatgatagta gcaaacgtaa cgtcatttta caaaaatggt
     6841 ttggtgctac tgggaagagt atccatatgg aacaacgttt tgtctgcgat tatggttgta
     6901 atatttatgt tggtgaaaac ttctacgcca attttaacca aactttctta gatgtttgtg
     6961 aaattcgtat tggcgataac tgtatgtttg gcccaaactg tcaactcctc accccactcc
     7021 atcctctaga tccaatagaa agaaattcag gattagaata cggtgcccca atccaaatcg
     7081 ggaataatgt ctggctaggt ggaggagtga ctattttacc aggtgttgta ttaggagata
     7141 atgtcgtcgt tggcgctgga agtgtcgtta cgaaatcatt tgaaaataat gttgttatag
     7201 caggaaaccc tgctaagata atcaaaaaat tag
//
LOCUS       DATRME010000091         7204 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRME010000091 DATRME010000000
VERSION     DATRME010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..372)
                     /locus_tag="VBC23_001858"
     CDS             complement(<1..372)
                     /locus_tag="VBC23_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6843783.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIII"
     gene            complement(374..1129)
                     /gene="mecA"
                     /locus_tag="VBC23_001859"
     CDS             complement(374..1129)
                     /gene="mecA"
                     /locus_tag="VBC23_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6843784.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            complement(1249..2088)
                     /locus_tag="VBC23_001860"
     CDS             complement(1249..2088)
                     /locus_tag="VBC23_001860"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6843785.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            complement(2134..4017)
                     /locus_tag="VBC23_001861"
     CDS             complement(2134..4017)
                     /locus_tag="VBC23_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6843786.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            4153..5703
                     /locus_tag="VBC23_001862"
     CDS             4153..5703
                     /locus_tag="VBC23_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6843787.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            5713..6453
                     /locus_tag="VBC23_001863"
     CDS             5713..6453
                     /locus_tag="VBC23_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6843788.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            6795..7025
                     /locus_tag="VBC23_001864"
     CDS             6795..7025
                     /locus_tag="VBC23_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843789.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
BASE COUNT         2443 a         1349 c         1130 g         2282 t
ORIGIN      
        1 tatgataata gctgcaataa taattcccaa cattttataa cgaggtctta attcaaagat
       61 gtcatctata aatcctgttg ttgtaactac gagaccgcca ataattacag gcaaaatgta
      121 gtgaaaataa gagacttcta taaagtgtct actggccgcc attggcatga aaaataatgt
      181 tgttacaaca aaggagagaa atattgctaa accaccacta cttggcattg gtactttgtt
      241 gattcttcga gcgttcggtt tatcaaccgc tcctaccttc aaagatataa ctcgaataat
      301 aggagttaga atgattgata ataaaaatgc tccaatcaat acaaaaatat actcaattgt
      361 aaaaggtatc attttatccc atctcaatca tttgtaattt tgaaatagca tcatcatgaa
      421 tcaattgaat actatgctct ttcaagtaag ctcgtgtttt tgtgcccaca ttagcatgtt
      481 caagcattcg agcatacatc aaattagcaa agtagcttgg ttggttttcc aagtctagca
      541 aaattgtcat atgatagcct ttaccatttt tatagagttc agaagcttct attggaaaat
      601 caatcgtttg tgaaaatcga actacagctt caatattatc aaaatcaaag acatagtgaa
      661 catatccaat cgtttctact tccttttcag gttgttcttc agaaacattt tcctcaacta
      721 cttcttgagt tgctttatcc atcatatttt caatttctgc taatttggca tgggcatccg
      781 tatccccttt ttccaacatc gattgttcca aggttttaaa aaagtcttct ggagacattt
      841 ttgaaatgtc acccaaatct gctaattctt ctaaatttaa atctttactt aattcagact
      901 ttgtaacaaa aacatcaatg cgatcttttt taggtgttac tcgaaaactt aacataccac
      961 tatttttaaa gttttctggc aagtctaatt catccatgac agaatagaaa aattcctcag
     1021 tcttctcctg agggattagg aaatctttca gctccatacc acgatcttct aaatcttcca
     1081 tactaattgt aattttcagt gttgtttcac tgatttgttt catttccata tcatcacctc
     1141 atacgagtat tcagttttct tattataccg aaaaagctat tttttagcaa tttttcacag
     1201 cctaacacgc taaaaagctg aggaatactc agctttttat gttatatttt agaataaatg
     1261 aacaactaac cagtagagga tgagtaaact tcctaagact atacgatact taccaaagat
     1321 ggtgaaatcg tgtcgtttga catagtctgt caagaaacga ataacatata aactaactac
     1381 gaaagctgtc agacttgcta ctaaaagtat taaagattgg tctaaactca agacgttacc
     1441 atctaaaaaa tatttaaccg ccttaagtcc actataacca aacatagttg ggatggcaag
     1501 gaagaaagta aagtcagcag cgaccgaacg actagttcca ataataattg ctcctaaaat
     1561 agtagctcca gaacgactgg ttcccggaac aatactgaga acctggaaac atccaatcaa
     1621 gaaagctgtc ttgtaagaca tacttgccaa ttcggttacc tgtggtttga ggtgtgcatt
     1681 acgtttttca acccatatga agacaaaacc ataaaagatt agggctattg caatcggaat
     1741 catgaaatta aaatgagctt caaaccaatt atcaaatggt aaagcaatta aaatagatgg
     1801 aatacaagct atcactactt ttaaccataa ttgccaagtt aaacgaattt ctctagcaga
     1861 tttacctggc tgaaatgggt taaggcgttt aaaataaatg acaataactg ccataattgc
     1921 cccaagttgt ataacaatat taaacatttc aacaaaggat ttgctttggt tcaacttcat
     1981 aaactcttga accaagatta aatggccagt actagaaaca ggtaaccatt ctgtcactcc
     2041 ctcaacgact cctaaaaata gggctttgag taattcaatt attaacataa taactccata
     2101 aatttttctt tattatacca tacttttgga aaattagaag gcaccgccac ctccgccgcc
     2161 tccgccgcct gagaaaccac ctgaagaacc gcctgaatta acactaaaat tagaagcgtg
     2221 actaacatct tcagttatcg ttgaaaaatg attactagat tgattgactg aaatcgcaaa
     2281 tcgatgacta tcaatgtttg caaatctttc tggaatatct atttggttca ctctgagtac
     2341 tttctcaaca cggtcagcgt agccgaataa agtagcgtaa accaatattc gattccataa
     2401 tacgatactt tctaactctg aacgatcaaa cgatttgata tcccttagca tgttcctaaa
     2461 actagtccat tggtagacac gacttgctcc atcttcagtt acaatataac cgttacttat
     2521 ttttcttgtc atcataaaaa tgattttatt caacaacagg ataacaccaa acaataagag
     2581 atagatacag aaataggtaa aataatttaa aaaaagcgta taaattataa caacaaaaga
     2641 aataagaggt gataggtaac tgaatctttt taacctagat aattctttag actcttctga
     2701 agacatttta cgataagggg atgaaattcc ctttcttcta agagagttta tattgtcttt
     2761 agaaatcaac cttgtgagac gcgtaattct tgataaggca ttacttcctc ggcgtctcat
     2821 ttgattttgc agatctgaag ccttgtgctg ctttttcagt tcacgtagtg tttctttata
     2881 actaaattgg taattagaaa agagttgatc tggcttcaaa ctcgtagaat ccgcaaaagc
     2941 aaactctata aaatctattt ctgcatcaga agctctatct aagttagcta gagataacaa
     3001 gttatcatca taattcaata cttttctatc aatcaagtct aatagaattg actgtatgag
     3061 ttgttcttga ctgataaggt gagttttctt ctcctcatct tgaaaatttt taaagctcat
     3121 actataaatg ctttgagtta actctaatgg tgaaaggtcc tcaggtgctt catataaatg
     3181 atgatcctta gggaattgcc cgtatttatt aacttttttt ctaaacagga acaccctaat
     3241 tgagatgaat agtgtcacaa taataaagaa tgaaggtaat aatatcctta acaagaaact
     3301 taggataaaa ccatgacgct ctattatctt ctcttgatgt tcaatttttt tcttgtaatt
     3361 atttttactg tttgtaggta gattaaaata agatctatcc caataaccat gaaattctaa
     3421 tcgtttgttt acattaaaag ctgtcaaatg gtaacgatta ttattttgtc ttattttagg
     3481 aggagtttta agataaccca agtgccccca aagacgagaa agagcaacct ttttgtcagt
     3541 tgatatccaa aaatctactt tctctaacgt tttatcccag tcgctaatag gaatccagtt
     3601 aagttcacca acatccttat gcataaatag aacatttttt agtttccact gaacattgac
     3661 tttaacagta tcacctgctt caccaccatt aaacactttc aaacggtagc catcctccaa
     3721 atcttctatc tggtaactaa ctttccttac tttaccatta attgaaactt caacctgtgg
     3781 cttattatta atatcaaaat tgtcaggtaa cttacccgcc gtacctaacg tgacatactg
     3841 tccattatag gacgaatcaa attggtaagt aaccttctct gtaaaatcag cactattatc
     3901 attgtgaata gttagattac cctcataatg aggaatgtta tagtccacct catctgcttg
     3961 aactgaaacc ataaaaaaac taagagctaa acaaatagcc aaaaaacatt tcttcattat
     4021 catcctccct cctttgtttg tataccattt taacataaaa atacaattaa tcgctttcaa
     4081 taagataatg atattgaaat cgaccttgtt tttcggtaaa ataggaggaa gtaacttatc
     4141 ggaggctgtt gaatgaaaaa aatattacta agtttattca ctgctttgct tatcaccttt
     4201 ggaggtatga caagtataca agcagatgaa tatctacgtg tcggtatgga ggcagcctat
     4261 gcgcctttca actggacaca aaatgataac acaaatggtg cagttcctat tgaaggaact
     4321 gatcaatatg ctaatggtta tgatgtacaa gtcgctaaaa agttagctaa aaagttaaat
     4381 aaaaaagttg ttgttgtcaa aacaaaatgg gaaggccttg ttccagcact aacttctgga
     4441 aaactcgata tgattattgc aggaatgagt cctaccgaag aacgtaaaaa agaaattaat
     4501 ttttcaaagc cttactacat ttcagaacct actctagttg ttaacgctga aggaaaatat
     4561 actaatgcta aaaatattag tgatttcaaa aatgctaaag taactgccca acaaggagtc
     4621 tacttataca acctcattga tcaaatcaat ggtgtcaaaa aagaagttgc catgggggac
     4681 tttaatcaat taagacaagc tgtagaatct ggtgttgtag acgcctatgt ttcagaacgt
     4741 ccagatgcaa cttccgctca aactgctaat cctaaattaa aaatgattga actgcaccaa
     4801 gggtttaaaa catctgatgc cgatacaaat atttccgttg ggatgcgtaa aggtgacaat
     4861 cgtattaatc aagtcaatca agttctcgaa agcatttcta gagataaaca aatcgccttg
     4921 atggataaga tgattaaaga acaaccatcc gttaaaaaag aaaaaaatgg aaaaccaaac
     4981 ttctttgaac agatggcaac tattttaaaa aacaatggtt ctcaattcct cagaggaaca
     5041 gctacaaccc tcctaatttc tatggtagga actatcgtcg gattatttat cggccttctc
     5101 atcggtgttt tccgtacagc accaaaatct gacaataagt taaaggctgc cttacaaaaa
     5161 ttattaggtt ggttacttaa catctatatt gaggtcttcc gcgggacacc tatgattgta
     5221 caatcaatgg ttatctacta cggaacagca caagctttcg gagtttcgct ggaccgtaca
     5281 ctagcagcta tatttattgt ttcaatcaat actggtgctt atatgagcga aattgtacgt
     5341 ggtggtattt tctctgttga taaaggacaa tttgaagccg caactgcact tggtttcacc
     5401 catggacaaa ccatgcgaaa aattgttcta ccacaagttg tacgtaacat actcccagct
     5461 actggtaatg agtttgttat caatatcaaa gatacctctg tactgaacgt tatctctgtt
     5521 gtcgaactct acttctctgg taatactgtt gcaacacaaa cctaccaata tttccaaaca
     5581 ttcaccatta ttgcgattat ttacttcatc ttaaccttca ccgtaacacg tatcttacgt
     5641 tacattgaaa aacgttttga ttcagataat tacacaaccg gtgcgaatca attacaagtc
     5701 taggaggttg gtatgacaca agctatttta gaaattaaac atttgaaaaa atcttacgga
     5761 agcaatgaag tgttaaaaga catttcgctt tcggtcaaca aaggcgaagt tatttctatc
     5821 attggttctt ctggatcagg aaaatcaact ttcctacgtt ctattaacct cttagaagaa
     5881 ccaagtggag gagaaatctt atatcacggt cacaatgttc ttgaaaaagg ctatgattta
     5941 aataattatc gtgaaaaact tgggatggtc tttcaatctt ttaacctctt tgagaattta
     6001 aacatcttag agaacgctat tgttgcacag actactgttc taaaacgtga acgccaagaa
     6061 gctgaaaaga ttgctaaaga aaacttaaat gctgttggca tgactgagca atattggaaa
     6121 gctaaaccaa aacaattatc aggtgggcaa aagcaacgtg tcgcaatcgc ccgagcactt
     6181 tctgttaacc cagaagctat actttttgat gaaccaactt cagccttgga tccagaaatg
     6241 gttggtgagg tccttaaaac aatgcaagat ctagctaaat ctggattaac aatgataatt
     6301 gtgacacacg agatggaatt tgctaaagaa gtatcagacc gcgttatttt catggacaaa
     6361 ggaatcattg cagagcaggg tactcctaag caacttttcg aaaatccaac acaagaaaga
     6421 actaaggaat ttctacaacg cttcttaaaa taaaatttat ccacatttaa atatagacta
     6481 tttatacccc tacaggtttt gtcattgtca tcattacaat ggaactggag gggatttttt
     6541 attaccacat ctttgatgaa atctacgact agcctcaact atagatcaaa ttagacagtc
     6601 gttattggat tgtatactat taaataagtt actaaatctc attttttaat tttaatcgat
     6661 atttaattaa ttatatattt gtttaaaatt aaaaacatgt tgttttaatg agcttttgat
     6721 gatataatat agtatgcaca aaatgaacta accactgtgc aagaatagtt tattatcttt
     6781 gaaaggattt tataatgaaa cttaaaatca gtgttttaac agcagcttca gctgtcactc
     6841 tattattagc tacttcagca agtcttgcga aggcagacac agtgtcttat ccttcacgtg
     6901 atgccggatt tatctactct cgtgggtatg cgccttctga atcaagctat ccttcacgcg
     6961 atgccgggtt tatctactct cgtggatacg ctccaaaaca cttcctaagt gcttacgata
     7021 aataaaaact gaccttatta acttaaccga tacactttca caagtataaa ttttcattca
     7081 attacattaa aaataattat ttagtttgct gaaaaacgat cactcatcga tcgttttttt
     7141 atatgcacaa tgatagccat tagctacttt ctcgttgggt attttcctca ttcatctttc
     7201 actt
//
LOCUS       DATRME010000092         6990 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRME010000092 DATRME010000000
VERSION     DATRME010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..781)
                     /locus_tag="VBC23_001865"
     CDS             complement(<1..781)
                     /locus_tag="VBC23_001865"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6843790.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHKA"
     gene            887..1477
                     /locus_tag="VBC23_001866"
     CDS             887..1477
                     /locus_tag="VBC23_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6843791.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMIDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            1803..2192
                     /locus_tag="VBC23_001867"
     CDS             1803..2192
                     /locus_tag="VBC23_001867"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6843792.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            2257..3306
                     /locus_tag="VBC23_001868"
     CDS             2257..3306
                     /locus_tag="VBC23_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6843793.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            3451..4986
                     /locus_tag="VBC23_001869"
     CDS             3451..4986
                     /locus_tag="VBC23_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843794.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            4979..6040
                     /locus_tag="VBC23_001870"
     CDS             4979..6040
                     /locus_tag="VBC23_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843795.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            6042..>6990
                     /locus_tag="VBC23_001871"
     CDS             6042..>6990
                     /locus_tag="VBC23_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843796.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIK"
BASE COUNT         2218 a         1015 c         1441 g         2316 t
ORIGIN      
        1 tagctttatg tggcggtatt tgtgtgaatt tatgatagta attactaggg tctgcttcgg
       61 ctaattgtag aagactgtta aatcgttgta aataccattc ttcaatttgt ttattttcag
      121 catcaatgta tattgaaaaa tcaaaataat cattcatgta aagtcgatgg ttttgttggt
      181 tttgaaagac atttattcct tctaaaatta aaaaatctgg tgtttcaata gtttgcaatt
      241 gatttggaac gatatcgtag atttcatgag aataaattgg gattttcgcg gttataccgt
      301 tctttatagt atctaagaaa tttagaagtg actccatatc ataggattcc gggaaacctt
      361 tacggtttaa aattccattt tgtatgagtt tctcgttagg atataaaaag ccatcagtag
      421 tgactaattc gacgtgacta tctttaaaag ttcgtgaaat tagtaattgt agtaagcgac
      481 tggtagttga ttttccaaca gcaactgacc ctgatatgcc gataataaaa ggacgttgat
      541 aattttcctt tttaagaaat attgctttag aaaaagataa attttcttga ctacgtttat
      601 agatttgaat aaggttaatt aaaggaaggt agatgtcaat aacatcttga atattaatat
      661 tatcgtttaa gcttttgata gattcaagct ctttttcagt tagtaaagct tgggattgct
      721 gatgtaagtc cttccaattt tctctggaaa tacggtcgaa attaataaat tcattattca
      781 tgtcaaactc ctatatgaaa gctattataa cattttattg agtgattgaa aaccgtttca
      841 aaatatggta aaataatatg gcgaaattgg agggttagaa ttatttatgg ctaatatgta
      901 ttatacagag aatcctaatg ttgaacatga tattcatgag ctaaatgtca aattattagg
      961 agaatcattc agttttttaa ctgatgcagg agttttctct aaaagaatga tagattatgg
     1021 tagtcaagtc cttctaaata gtttacattt tgaaaaaaat aaatccttgt tagaccttgg
     1081 gtgtggatac ggtccgctgg gaatttctct agccaaagtg caaggagtta aagcaactat
     1141 gattgacatt aatacgagag cattggaatt agctaagaaa aatgcaacac gcaatggagt
     1201 agtagttgaa gtctttcagt caaacattta tgaaaatatt tctaaaactt tcgactatat
     1261 tataagcaac ccacctattc gtgctggaaa gcaagttgtt cattctatta tcgaagaaag
     1321 tatctgttac cttaatacag gcggtagcct taccattgtc attcaaaaga aacaaggcgc
     1381 tccaagtgca aaagctaaaa tgctagatac ttttggtaat tgcgatattt taaaaaaaga
     1441 taagggatat tatatattga gaagtgaaaa agtctaaatg atatccctaa agcgtaaaaa
     1501 taataaatag ttcggataat aactatcgta ttattttttg gaaaggtttt ctctaaaaat
     1561 gttttcttta aattccgtat aagataaaaa gaattctgct ttttattctg aaaattataa
     1621 aaaactactt acattatcta aaaaaaatgg taaaatagat tcagctaaaa taccaagact
     1681 aatgaaacta caagacttgg ttactcaata ataaatactt ctgtgtttga taagaagata
     1741 ataaacgtgt tctaaaaatt ttgccagtaa acattatgaa gcgaaaggtg cagctactaa
     1801 aaatgggaaa tatagagttg aaaaaattgg ctgtaaaagc gagtgaaaat gcatacgttc
     1861 cttactcgaa atttccagta ggcgcagcat tgcgtacagc agaaggaaaa atatttacag
     1921 gatgtaacgt tgaaaatatc agttatggat tagcaaactg tgctgagcgt actgcaattt
     1981 ttaaagctgt ttcagaaggt tataaagatt tttcggaaat tgctatttat ggaaatactg
     2041 aagaaccaat ttctccttgc ggtgcatgcc gtcaagtgat ggtggaattt tttaataaga
     2101 atgctaaagt tacattgatt gctaaaaatg gtaagacggt cgagacgacg gtcggtgagt
     2161 tgttaccata ctcgtttgtt gatttaaaat aatttaaatc agttgctctt ttaagcattt
     2221 agcttaaata ttaagttttt taggagggtt catttcatga acaaaaaaat tgccgggatc
     2281 ggcttggctt cgattgcagt acttagttta gctgcatgtg gacatcgtgg tgcttctaaa
     2341 tctggtggta aatcagatag cttgaaggtt gcaatggtaa cagataccgg tggtgttgat
     2401 gataaatcat ttaaccaatc tggttgggaa ggtatgcaag cttggggcaa gaagaatggc
     2461 cttaaaaaag gagctggttt tgactatttc caatcggcaa gtgaatctga ttatgcaact
     2521 aacttagata cagctgtgtc tagtggttat aaattgattt tcggtattgg attttctctt
     2581 catgatgcta ttgataaagc agcagacaat aacaaagatg ttaattacgt catcgttgat
     2641 gatgttatta aagggaaaga taatgttgca agtgttgtct ttgcggataa tgaatcagct
     2701 tacttagcag gtattgcagc cgctaaaact accaaaacaa aaacagttgg ctttgtaggt
     2761 ggtatggaat ctgaggttat tacccgtttt gaaaaaggtt ttgaagcagg tgtcaaatca
     2821 gttgataaat caattaaaat taaagttgac tatgctggtt cattcggtga tgctgctaag
     2881 ggtaagacaa ttgcagccgc acaatatgct tctggcgcag atattgttta tcaagtagct
     2941 ggtggaactg gtgcaggtgt attcagtgaa gcaaaatcac gcaatgaatc tttaaaagaa
     3001 gctgacaaag tttgggtatt aggtgtagac cgtgaccaag cagcagaagg taagtatact
     3061 tctaaagatg gtaaggcttc taactttgtt ttggcttcat caattaaaga agttggaaaa
     3121 tcagttgaat tgattgctac taaaacaagt aaaggtaaat tcccaggtgg taatgtaaca
     3181 acttatggtt tgaaggatgg tggagttgat atagcaacta caaacctttc agatgacgcc
     3241 gttaaagcga ttaaagaggc taaagctaaa attatttcag gagatatcaa agttcctagc
     3301 aaataatatc tttaagaact ttcaaaaaag cgattagaaa tcgctttttt gaagctgaga
     3361 taaacttata tctcagtaaa atccatcagt cgttaacgat tggtggattt tactgaacta
     3421 taaatgaatt tttctgaaag ggagaaccac atgacacaga atgtcattga aatgaaagaa
     3481 atcactaaga aattcggtga ttttgtggca aatgatcata tcaatttgac ggttgaaaaa
     3541 ggtgagatac atgctctgct tggcgaaaac ggtgcaggga aatcaacttt aatgaacatg
     3601 ttagcaggtt tactagaacc aacagacggt caaattttta ttaatggtca gcctgtaact
     3661 attgattcac cttccaaatc gtcacaattg gggattggta tggtacatca gcatttcatg
     3721 cttgttgaag catttactgt agctgaaaat attgttctag gaaatgaaac cacccaaaat
     3781 ggtgttttag acattaagac agcagctaaa gaaataaagg aactatcaga gaaatatggt
     3841 ttgtcagtaa atccaaatgc taaaatatct gatatttctg taggagcaca gcagcgtgtt
     3901 gaaattttga agacacttta tcgcggtgca gatattttaa tttttgatga gccgacggcg
     3961 gtgcttacac catcagaaat aaaagaattg atgacaatta tgaagagcct tgttaaagaa
     4021 ggcaaatcca ttatactaat tacccataaa ttggatgaaa ttcgggcagt tgctgataaa
     4081 gttactgtga ttcgccgtgg taaaagtatt gaaacagtcc ctgttgcagg cgcaagttct
     4141 caacaactgg cggaaatgat ggttggacga tctgtttcct tcagaactga aaaaaaggaa
     4201 gctaatccga ctgacataat cctttctgta aaggatttag ttgtagaaga aaatagaggc
     4261 ggcgttctag ctgttaaaaa tctgtcgtta gatgttcgag caggtgaaat tgtaggtata
     4321 gctggtattg atggtaatgg acaaagtgaa ttaattcaag ctattacagg tttaagaaag
     4381 gtaacttctg gtcaaattgt aattaaaggt aaagatgtca caaaattttc aagtcgacaa
     4441 attacagaat taagtgtagg tcatgttcca gaagatcgtc atagagatgg tttggttcta
     4501 gatatgacga tggcagaaaa tttagcttta caaacttatt ataaagagcc attgagtcat
     4561 aaaggtatcc ttaattttgc taaaattaaa gagtatgctc gtcaattaat gacagaattt
     4621 gatgtgcgtg gtgctggtga acatgtttta gcaagaggat tttctggtgg taaccaacaa
     4681 aaagctatta ttgctcgtga agtggatcgc gatccagacc tactcattgt tagccaaccg
     4741 actcgtggtc tagatgttgg tgctatcgag tatattcata aacgtttgat tgaagagcgc
     4801 gataaaggga aagcggtctt agtagttagc tttgagttag atgaaatctt aaacttatca
     4861 gatcgtattg cagttattca tgatggtaaa attcaaggaa ttgttaaacc cgaccaaacc
     4921 aacaagcaag aattgggtat tttgatggct ggtggaaaaa ttgaaaagga ggagagagat
     4981 gtctaagaag gcgcaaaaaa ttgcagttcc tctgatttca gttgtactag gaatcattct
     5041 aggagctatt attatgctga tatttggtta tgatccactt tggggttatg agggattgtt
     5101 ccaaacagca ttcggtagta taaaaaatat tggtgaaatt ttccgtgcaa tgggaccatt
     5161 aattttaatt gctctaggtt tctcagttgc cagtcgtgca ggtttcttta atattggact
     5221 tccagggcaa gccttatcag gatggattgc cgcaggttgg ttcgctcttt cacacccaga
     5281 tatgccacgt ccagcaatga ttctttgtac tattattatt ggtatcgttg ctgggggaat
     5341 cactggagct attccaggta ttctcagagc ataccttgga acaagtgaag ttattgtaac
     5401 tattatgatg aactatattg ttctttattc aggaaatgct attgtacaac gtgttttccc
     5461 aaaaagcatt atgcgtacaa gtgactcgag tgtatatgtt tctgccaatg catcatacca
     5521 gacagattgg ttatctagtt taacgaataa ttcacgtatc aatattggta tcttcattgc
     5581 tattattgca gttgtgttag tttggttcct attaaataaa acaacgcttg gatttgaaat
     5641 tcgttcagtt ggtttaaatc caaatgctag tgagtacgct ggtatgtctg caaagcgtac
     5701 aattattcta tcaatgatta tttctggagc ttttgcagga cttggtggtg ttgttgaagg
     5761 actaggtaca tttgagaatg tatttgtaca gccatcctcg ttagctattg gctttgacgg
     5821 tatggcagta agtctactcg cagcgaatag tccaataggt attttatttg cagcatttct
     5881 atttggtgtt ctaagtgttg gtgcgcctgg tatgaacatt gcaggtattc cacctgagtt
     5941 aattaaagtt gtaacagctt caatcatctt cttcgtaggt gtccattaca ttattgagta
     6001 tgttattaaa cctaaaaaac aaatgaaagg tggtaagtaa attgagttta acaacgattt
     6061 ttgcattgct attttcatca atgctaattt acgcaacacc tcttatcttt acaagtattg
     6121 ggggaacctt ctctgaacgt ggtggtatcg tcaacgttgg tttagaagga attatggtaa
     6181 ttggagcttt ctcaggcgtt gtatttaatt tagagtttgc ttctgttttt ggtgatgcga
     6241 ctccatggat ttctgtacta gttggtggtt tagttggttt aattttttct gttatccatg
     6301 cagttgcgac agttaatttt cgtgcagatc atattatcag cggaaccgtt cttaatttaa
     6361 tggcaccttc attagctgtt ttcttgatta aggttcttta taataaaggt caaactgata
     6421 atattcaaga atcatttgga aaatttaatt tccctatact atcagatatt ccttttgttg
     6481 gtgatatctt ctttaaagga acaagcttag tcggttatat tgctattctt ttctcattcc
     6541 ttgcttggtt tattctatat aaaactcgct ttggattaag attacgctca gttggagaac
     6601 atcctcaagc agcagacacc ttaggtatta atgtttatct tatgcgttac tctggtgtct
     6661 tgatttcagg tttccttggt ggaataggag gagctgtata tgctcaatct atttctgtta
     6721 actttgcagc gacaactata ttaggaccgg gctttatctc cttagcggcg atgatttttg
     6781 gtaagtggaa tccaattggt gcaatgcttg ctagtctatt ctttggttta tcgcaaagtt
     6841 tagcagttat cggtagccat ttaccacttt tgagcaatat tccaacagtt tatctacaaa
     6901 ttgctccata tgtcctcact atcattgtat tagcagcttt ctttggacaa gcagtagctc
     6961 ctaaggcgga tggtattaat tatattaaaa
//
LOCUS       DATRME010000093         6732 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRME010000093 DATRME010000000
VERSION     DATRME010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBC23_001872"
     CDS             212..1288
                     /locus_tag="VBC23_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843797.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
     gene            1290..1496
                     /locus_tag="VBC23_001873"
     CDS             1290..1496
                     /locus_tag="VBC23_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6843798.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            1493..2395
                     /locus_tag="VBC23_001874"
     CDS             1493..2395
                     /locus_tag="VBC23_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843799.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            2392..3153
                     /locus_tag="VBC23_001875"
     CDS             2392..3153
                     /locus_tag="VBC23_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843800.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
     gene            3322..3810
                     /locus_tag="VBC23_001876"
     CDS             3322..3810
                     /locus_tag="VBC23_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843801.1"
                     /translation="MEIKEYENNPYHLAQLVDLINYCQNIEAKLDIKMAEQDDIFQIE
                     NYYQNRKGQFWIALENEKVVGSIALLRIDDKTAVLKKFFTYPKYRGNPVRLGRKLFER
                     FMLFARASKFTRIVLDTPEKEKRSHFFYENQGFKQITRDKLDVDYIFPDRDSRIYVKL
                     LD"
     gene            4332..4667
                     /locus_tag="VBC23_001877"
     CDS             4332..4667
                     /locus_tag="VBC23_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843802.1"
                     /translation="MKRNKHLPLTETTYYILLALFEEAHGYAIMKKVEEMSGGDVRIA
                     AGTMYGAIENLLKQKWIKSISSDDRRRKVYIITETGKEIVELETNRLRKLLNTANQLG
                     FGGDGYDKV"
     gene            4654..5262
                     /locus_tag="VBC23_001878"
     CDS             4654..5262
                     /locus_tag="VBC23_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO6843803.1"
                     /translation="MIKFKLFFKSTEKEEEWIDKVQKSGYTFTGINSFLPAFYYFKRD
                     KEKDKMSIKMDFKKYMSKDDYTNYITLFEDSGWKHISGSRWGGLHYFQKIKPETSSEI
                     FSDQESKSAMAMSYYKYTCEHAFFSLFYFFLLIRTTSITIFDPKSWYLTQGLWEMQGK
                     IFWLGFLFETPFALLRILPMFYFLISGIYYITRAVKPKEIIN"
     gene            5704..6096
                     /locus_tag="VBC23_001879"
     CDS             5704..6096
                     /locus_tag="VBC23_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO6843804.1"
                     /translation="MQSQKLNILQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQAFSWLIDELDFRGFDEELGWIHVFAHGADLLTEIVKMK
                     CFSKDDSSKILTIIKRVMITDDILTLNG"
     gene            6275..>6732
                     /locus_tag="VBC23_001880"
     CDS             6275..>6732
                     /locus_tag="VBC23_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO6843805.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDYD
                     IVFVTNFIEDIISDTNYHKKFGDILIMQKPNEFRNKTEYNCFAYLMQFQDLTRIDLRL
                     IKPEFLEDYLDDAFSKVLLDKKNKYLDYNFERSSLYETKQLSEDEINKIL"
BASE COUNT         2478 a          850 c         1162 g         2242 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaata tgaatacaga aataccaaat caattattac
     1321 cattcctagt tccgttgatt atgttgcaag gagctttaat tatcatttct ctagtcaaac
     1381 taagtaaact taagatgact aaacaccttt caaagccagt ttggtttttg gtcattatat
     1441 ttcttaatat aattgggcca attgcatttc ttattttgga agggaataat gaatgaaagc
     1501 tatctcattt caaaacgtta caaaatcttt tggtcctaaa aaaatattaa ataatgtttc
     1561 ttttgattta gaagaaaata tgatttatgg attcgtagga ccaaatggag caggtaaaac
     1621 aacgacaata aaaatgattt taggattgtt aaaatttgat acgggtttca taacaatttt
     1681 tggtaaaaaa gttaattttg gaagaacaga taccaatcaa ctgattggct atctcccaga
     1741 tgttcccgag tactacgatt atatgacagc tctagaatat cttgatttat gttctggttt
     1801 agcgagatca aagcataagc tttctaacaa agaactactt aggtcagttg gacttgacga
     1861 taatcaccaa aagattgcta cttattctcg aggaatgaaa caacgtcttg gattagccca
     1921 agcactggtc catgatccta aaattattat ttgcgatgaa ccaacctctg cattagaccc
     1981 gaaaggtcgc caagatattt tagatattat ttcaaatctt agaggagaaa aaactgtgat
     2041 tttttctacc catattctct ctgatgtcga aaaaatatgt gatcacgttc tggtgttaac
     2101 taagtgtggg atttattctc ttgaagagtt aaaaggaaaa aaatcagagg aaaattattc
     2161 ggttcgtatt ttaataaaag tgacaaagtc tgaagctaag gtactatctc ataactatca
     2221 gatagaaaag aaagataatg agtatgcact gactctcaaa ggctctaaaa tggataacaa
     2281 agcagattta ctagctggtt tttaccaaga tttagtttct ttaaagatta gcccatcagc
     2341 tattgaagtc attgacaata gtttagagga gctttatttg gaggttacca aatgaaatct
     2401 ttagcagtaa tgttaaaaaa agaatggatg gagaatgtta ggacatacaa agttatatct
     2461 attctgatta cttgttcaat atttggtata ttaggtccct taactgcact tatgatgcct
     2521 gatattatgg caggaatttt acctaaaaaa ctacagggag cgataccaga gactacctat
     2581 attgattctt atattcagta ttttaaaaat atgaatcagc ttggtttagt tattttagta
     2641 tttttattta gtagtacact gacgcaagaa ttttcaaaag gaaccttaat taatctagtt
     2701 acgaagggat tagccaaaaa agttataatc cttgctaaat ttatagtaat tacccttcta
     2761 tggacagtgt catacttatt aagtgttgtc atccatttta gttatacgtt atattacttt
     2821 agtaacgaag gaagtcataa attaatggtt tacggtgcaa catggtttat cggtattctt
     2881 ttcataagct taatactctt tttttcagtt ctttttcgta aaactttagg gattttgtta
     2941 ggttgtcttt ttatggtggt gttatgtgta gtatctggat tttttaaaat cagtaaaagc
     3001 tataatccta tgcttcttat ccaaaaacaa ggagaaatgt taagtggaaa gtttaaagtt
     3061 caagacttaa tggaaccgag catattagtg tttttgctta ttataatgac gttattactt
     3121 agcatgtaca tttttgaaaa aagtgaagtt taattttgat aagatatcaa taaggcgata
     3181 gctacttttt gaaaaaataa aaccgaatta agagctttgc tgctatttga cttatctaga
     3241 tcaatatttc caactaatat gatatcctat gcatatctca ttgcagaaat atactttaaa
     3301 gtcaaaataa ggaaatatat tatggaaatt aaagaatacg agaataatcc gtaccattta
     3361 gcacagctag tagatttaat taattattgt cagaatatag aagctaaact tgatataaag
     3421 atggctgaac aagacgatat ttttcaaata gaaaattatt atcaaaacag aaaaggtcaa
     3481 ttttggattg cattagaaaa tgaaaaagta gttggttcga ttgcgttact gcgtattgat
     3541 gataaaacag ctgttctaaa aaaatttttt acatatccaa aatatagagg aaatcctgtt
     3601 cgactcggtc gaaaattatt tgaaagattt atgctttttg ctcgagctag taaattcaca
     3661 agaattgtct tggatactcc agaaaaagag aaaagatctc acttctttta tgaaaatcaa
     3721 ggatttaagc aaataacaag agataaacta gatgtagact acatttttcc agatagagat
     3781 tcaaggatat atgtaaaact tcttgattaa ttgtgaagaa aactagccaa acagatgaat
     3841 aaacccaaat aacgattatt ttagataata cattggaaaa gtggttgtag aatatagaaa
     3901 aagaaatttt taggattacg gttatcattg gttatggtag cttattcaag taatatcata
     3961 agagatgaat caatggatat aaattgaata aaaaataata gaggccagag tgatgaactc
     4021 tagtctctat tattaatttt aaaaactatt gtgtttatga ggaggtattt taactgtata
     4081 gattggataa gaagttataa ttaaaggtat taagatcagg atgagatgaa taaacaacag
     4141 tactatagtc aatagattat aaaaaactat agtacttttg agaagttacg actaaaataa
     4201 aaaactccaa acgaataatt ggataggcta tctgttcttt tctttttaat aagtttaaat
     4261 atacagttga cctatatagt tatattatat agaataacta tataaagttt aactgtatag
     4321 aaagaaaatt gatgaaaagg aataaacatt taccgttaac agaaactacc tattatattt
     4381 tattagcttt gtttgaggaa gcgcatggct atgctattat gaaaaaagtt gaagaaatga
     4441 gtggcggtga tgttagaata gccgcaggga caatgtacgg tgccattgaa aatttactta
     4501 aacaaaaatg gataaagtct atctcaagtg acgatagaag aagaaaagtt tatattatta
     4561 ctgagacagg aaaagaaata gtagaacttg aaacgaatcg attaagaaag ttacttaata
     4621 ctgctaatca gttgggtttt ggaggagatg gttatgataa agtttaaatt attttttaaa
     4681 tccacagaaa aagaggaaga atggatcgat aaggttcaaa aaagtggcta tacttttaca
     4741 ggcattaact catttcttcc agccttctat tattttaaga gagataaaga aaaagacaag
     4801 atgtcaataa aaatggattt taaaaaatac atgtcgaaag atgactatac taactatatc
     4861 actttatttg aggacagtgg atggaagcat attagcggta gtcgttgggg agggctgcat
     4921 tattttcaaa aaataaagcc tgaaaccagt tcagagattt tttctgatca agaatcaaaa
     4981 tcagcgatgg ctatgagtta ttataaatat acttgtgagc atgcgttttt ctctttattt
     5041 tatttctttc tcttgataag aacaacttct atcaccatat ttgatccaaa aagttggtat
     5101 ctgacgcaag gtctatggga aatgcaaggg aaaatatttt ggttagggtt cttatttgaa
     5161 acaccatttg ccttattaag aatattacct atgttttact ttttaatttc tggaatatac
     5221 tatattacac gagctgtaaa acccaaggaa attataaatt aagcagaagt attttagatt
     5281 tttatacttt tatactttct atattctctg gtaattttag ttctatcgta tatttatggt
     5341 atgcctcaat tttttcctaa tagaggttac aaggtaggcc ttcgctatat ttctagagtc
     5401 aaagaataag tactaaaaat ttctgatata aaagtgagta tccaagtaaa aaattaaagt
     5461 tactataatc attttaaagt gaaaacgaaa actctaatgt tacactttgg aaaatataaa
     5521 atataaagtc ctgcaaggag aaacatggaa gcaagtgatg taatcaaaaa gatagaaaat
     5581 aattatccaa ttgatgagaa tgttttatct ttttgattac atcatatagg agattggaac
     5641 ccgagagttc gagatgaaat agtatatggg tcttgggtta aattagtaat agaacataaa
     5701 ctgatgcaaa gccagaaatt aaatatcctt cagagagttt taagggataa atttctttta
     5761 caaggattag gagtacccaa cagtaatact gtgttaaaca gatcttttac tgctctatta
     5821 ctagctttat tgttggagga ctcaaataat aaaagttgga tatctgaaga agatcagata
     5881 aaaattatga atcaggcatt ctcctggctt atagatgaac ttgattttag aggctttgac
     5941 gaagagcttg gatggataca tgtctttgct catggagctg atttattaac agaaatagtc
     6001 aagatgaaat gtttttcaaa agatgacagt tcaaaaatct taactatcat aaaaagagtg
     6061 atgataactg atgacatact aacattaaac ggttgaaaat attttgaatc ataatcagtt
     6121 gttcagaaaa atttaaaatt gaatccttgg aggtcgtaaa ggtatttcca tgctaattta
     6181 agtttatgat tgcatgctta gagaaaaata caagggtaat ataaaggtat tagaaaaaat
     6241 atggtaatat aaagtgaagt aggaggtagc cattatgaga gatgagcaag aaatatataa
     6301 tttggtttta aacatagcaa atcaagataa gagaatcgaa gctgtactat tgaatggatc
     6361 tcgggctaac ccaaatgtac cgaaagatga ttttcaagat tatgatattg tttttgttac
     6421 taattttata gaagatatta tttctgatac taattatcat aaaaaattcg gtgatattct
     6481 tataatgcag aaaccaaatg agtttagaaa taagacggaa tacaattgct ttgcttactt
     6541 aatgcagttt caagatctga cgaggattga tttaagactt attaaaccag aattcttgga
     6601 ggattattta gatgatgctt tttcaaaagt acttttagat aaaaagaata aatatcttga
     6661 ttataatttt gaaagaagtt ctctgtacga aacaaagcaa ttgtcagaag atgaaatcaa
     6721 caaaattctt aa
//
LOCUS       DATRME010000094         6679 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRME010000094 DATRME010000000
VERSION     DATRME010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            73..1266
                     /locus_tag="VBC23_001881"
     CDS             73..1266
                     /locus_tag="VBC23_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6843806.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELR
                     SEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFY
                     TQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     KPKTI"
     gene            complement(1461..1985)
                     /locus_tag="VBC23_001882"
     CDS             complement(1461..1985)
                     /locus_tag="VBC23_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6843807.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            2117..2980
                     /locus_tag="VBC23_001883"
     CDS             2117..2980
                     /locus_tag="VBC23_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6843808.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            3065..3919
                     /locus_tag="VBC23_001884"
     CDS             3065..3919
                     /locus_tag="VBC23_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6843809.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            4029..5056
                     /locus_tag="VBC23_001885"
                     /pseudo
     CDS             4029..5056
                     /locus_tag="VBC23_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            5072..5461
                     /locus_tag="VBC23_001886"
     CDS             5072..5461
                     /locus_tag="VBC23_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6843810.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            5471..5866
                     /locus_tag="VBC23_001887"
     CDS             5471..5866
                     /locus_tag="VBC23_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6843811.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            5894..6211
                     /locus_tag="VBC23_001888"
                     /pseudo
     CDS             5894..6211
                     /locus_tag="VBC23_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            6298..6582
                     /locus_tag="VBC23_001889"
     CDS             6298..6582
                     /locus_tag="VBC23_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6843812.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
BASE COUNT         2301 a         1080 c         1148 g         2150 t
ORIGIN      
        1 taattattct gttgacttta aagaaatatt ctctaaaata gataacatat taaatcaaag
       61 gtggtaactc atatgattga ttttctactg ttgaaatcat tacacagtct tctaggtcta
      121 actattactg tttgtgatca aaatttttct gttatcagag agtataaatc cgagaaaact
      181 atttcacttt tttacaatca ttaccttatt ttaagtaact ttagtaaaac tcaacatgat
      241 tttttatttc attatggctc tttaggagaa ctttttcttg tccaccatat tcaacaatat
      301 tatattatca tcggtccctg gcgtagcaat gttattgacc ctttacttct taagaaaaag
      361 ctgactgaaa cacaaatcaa tgctagtgaa caagactact ttattgatag actatcccaa
      421 ttacctttct tctcacttag tcaaatccga gaattactaa tagtaaccaa ttattgtcta
      481 actggggttg tgaaagataa attatcagag cccttacatt actacacgaa agggtggagc
      541 aactcctttg atctagataa aattaagcaa ttttctaaac agaatatgag ttcttacaaa
      601 tatcagtatc attttgaaaa taatattcta aaagctgtta aatcaggtag tgaatttctt
      661 ttgaaagaaa cagtggaaca atttagcaat tctatcgtac ctataatcag tggagatgaa
      721 ttgcgatctg aaaaaaacta ctccatcatg atttatgatc gtctctcaca ggctaccatc
      781 caagcaggac ttgatatcga aacagcttat cgagcacgag atcgttttat aaaagaaaac
      841 gagtcaacta taagtctaaa tgaagtttta aaattacgtg atactgctat cttattctat
      901 actcaacaag ttcattcttt aaaaaaacat ctcgaaaccc ctcattctca aactattgtc
      961 gcagtgattc gatatcttga aaataatcta aatcgcttta ttaaaacaga agaaatagct
     1021 aaagaatgtc acatgagtga atcgaagtta cgaaaactat ttaagcaaga aaaacacatc
     1081 actattcaac aatattttct aaacttaaaa atcgaagctg ctaagcaatt actagatgaa
     1141 aataagaaag tagaagaagt ttccaattta cttggatttt ccacttcttc taatttttca
     1201 aggacattta aaaaaatagt gggaattagc ccactagaat ataagcaaaa gcctaagaca
     1261 atatagtaat cgaaaaatat tcattttgct tatggtgaca tcaacaatcc caaagaaact
     1321 ctttcatcaa ggggactttt ttatctcccg aattatactc atttaatcta ataatttcaa
     1381 ctcgctatat tagacaatca atccattcat cacctctgat aacgctaatg aaataagaga
     1441 atctcccaac tctctataat ttacctctct accatagtta aataatattt tagtaaatct
     1501 tcaacagtca ttttctgtct ttgatgaagc caccacttta aggtctcgat aaaactggat
     1561 aaaagaaatt gttttaagaa atcttcagga atgtccacct ttgtaatata ctcctcacgc
     1621 aatctagggt aaacatcgtg ttctaattca cttctaaagc gtaataaaaa atagggatcg
     1681 tcagataata ataacgtagc gatactatct tgattttgtt caaaatgttt taagatatga
     1741 actaaatact cttcaaacgt aacatctcta ccctgcttaa ataagtggtg aaaaaggtct
     1801 tcacagagct ccttgagtaa cacttctttg ctttcatagt gagagtaaaa agttgatctt
     1861 cctacatttg caagtccgat tacatcttga accgttattt tagaataatc attttcctgt
     1921 agcaagctta taaaagcatt ataaatcgct acttttgttt tctgaatttg tctatctttt
     1981 accatatagg acactttcta agttttgttc agtattgaac aagtgttctt ttttgtttct
     2041 agcaattctc ttttatctcc tagataatag aagaaagaat cacactacaa taccagtata
     2101 acaaaaggag aaaaatatga catcaaacaa aaaagtggct atcgctttta tattaaatat
     2161 cagcttttcc gtccttgaat tcatctttgg aagtctattc ttctcaggag ctatcttagc
     2221 cgatgctgtt catgattttg gtgatgcaat agctattggc atctcagcta ctttagaaaa
     2281 aaaatctaag aaagatgaag atacgatatt ttcattaggt tataaacgtt ttagcctttt
     2341 aggagctctt atcacaagtc taattcttat aagtggatct atcttagtca tgatagaaaa
     2401 tattccaaaa ttgtggcatc ctactcctgt taattatcac ggcatgttta ttctagctgt
     2461 catcgctatt atcattaatg gtctagctag ttttattctc cattctggtc aaagtaaaca
     2521 tgaggaaata ttaagccttc attttttgga agatattcta gggtggttag ctattattgt
     2581 tatatctctt atcttaaatt ggaaaccgtt gtatatcctt gatccattat tatcagttgc
     2641 catatctact ttcatcttat ctaaagcact tcctaaatta ctatcaaccc tgaaactttt
     2701 tttagatggt gttccagatt ccatagatta cgctgcatta catgatgaac ttaagggact
     2761 ttcgcaagta agatctatca atcaactcaa tatctggtca atggatggta ttgataatcg
     2821 agcaatcatt cactgttgct taaatcaatt gatatctgaa aaagattgta aaagagctat
     2881 tcgaacgatt tgccaacatt ataagataaa cgatgttact gttgaaatcg attattcttt
     2941 aagagaacat caaaatcatt gcaaaccatt aaaaaactaa cctgagtgtt agttttttcc
     3001 ttgacttgga gttaactcta agtgttaaga ttaggtcaat actaatgaaa gaaggaaaat
     3061 aaagatgaca gatacaatga tattaaatga caatcataaa ataccaactt taggtttagg
     3121 tacgtggatg atagataatg atagggtagc tcaagttgtt aaagatgcta tcgaattagg
     3181 ctaccgccat attgatactg cacaagcata caacaatgaa aaaggggtcg gtgtagggat
     3241 taaagaatct ggtattacac gtgaatcttt atttgttacg actaaaattg ccgctgaaca
     3301 taaagattat gaaagtgcta caaaatcaat tgatcgatca ctagaagctt tagggcttga
     3361 ttatattgat ttaatgatta ttcatagtcc acagccttgg aaggaatggc gtgaaacaga
     3421 taaacatttt gaccaaggaa atttagaagc ttggagagct ttagaggatg ctcaaaaatc
     3481 tggtaaggta aaatccatcg gtgtctcaaa ctttttagaa aaagacttag aaaatatctt
     3541 aaaaaatggt tatgttaaac ctgctatcaa tcaaatctta gctcacatcg gaaatacacc
     3601 tttcgacttg attgattact gtcaaagtaa aggtattcaa gttgaagctt attcaccaat
     3661 tgcacatgga caagcattga aatctgatgg tattcaaaaa atggctgaaa aatatggggt
     3721 tagtgttgct caattatgta ttcaatattt actacaatta aacctgatcg ttttaccaaa
     3781 ggcttctagt aaagaacatt tacagtctaa tctagacttt gactttgtaa ttagtgacga
     3841 agatatgtcg atattaaaat cacttatgtt tgacgattat ggggaatttt ctaacttccc
     3901 tgtttttagt ggaaaatgaa accatatgcg gatttttaca gatgtttaat tttcaaaatt
     3961 tttaagtttt tcttgacttg aagttaactc taagagttat aatgaagtta tagaaaagag
     4021 gtaaggttat gaaagtagct acatttattg aacctggcaa aatggttata actgatacac
     4081 caaaaccagt cattgaacaa gagacagatg ctgttatcaa aattgttaga gcctgtgttt
     4141 gtggttcaga cttgtggtgg tatcgtggta tttcaaaacg tgaaagtggt tcttttgcag
     4201 gtcatgaggc tattggtatc gttgaggaag ttggtactaa agtaactgac gtgtcaaaag
     4261 gtgattttgt tattgttccc tttacacatg gctgtggcca atgtccgtct tgtaaggctg
     4321 gatttgatgg aaattgtaca aatcatcaag ctgaaaaaaa tgtagatacc aaggtcaata
     4381 tctacgttat actaatgcaa actgggcatt ggttaaaatt cctggtcaac cttctgacta
     4441 tgataatgag acacttaact ctcttctcac cttatcagat gtaatggcaa ctggttatca
     4501 tgctgcagaa gttaaagaag gtgatacggt agttgtcatg ggagatggtg ctgttggact
     4561 ctgtggtgtt attgctgcta aaatgttagg tgctaaccgt atcattgcaa tgagtcgtca
     4621 caaagatagg caggaactag cattgacttt tggtgcaacg gatattgtcg aagaacgggg
     4681 tgacgaagcc gttaaacgtg ttttagattt aaccaatcaa gcaggtgccg atgctatttt
     4741 agaatgcgtt ggtacagagc agtctgttga tacagctacc caaattgcta ggcctggtgc
     4801 tgtcatagga cgtgttggta tcccacaaaa tccagacatg aataccaata atttattctg
     4861 gaaaaatatc ggccttagag gtggcattgc ttctgtaaca acatttgata agtctgtcct
     4921 tttagatgct gttctaactc ataaaattaa cccaggctta gtttttacaa aatcctttgt
     4981 attagatgat attcaaaagg cttatgaagc aatggataaa cgtgacgcta tcaaatcctt
     5041 agtgattgtt gactgaatat cgatgttaaa aatgcatatc aaagaagtta gtaaaaagac
     5101 tggcattacg tcagatacca ttaggtatta tgagagaatt aatcttattc cccctattca
     5161 tcggagtgaa tcaggaattc gtgagttcac acaaaacgat attgatattt tagaatttgt
     5221 ccgctatttt agaggcgcag gtgtttccgt tgaaagtctc attgattata ttcatttggt
     5281 tgaaaaaggc gattccacaa tcccagctag gctagaaatt ttaaaagata aacgcaacaa
     5341 ccttgaagat aggcttcaag gcctccaaga agcacttaat cgtctaaatc acaaaataga
     5401 caattatcaa aaagttcttg ttcctagaga acaagaactt tttgatccaa caacgaaatg
     5461 aggaaatctt atgcaagaaa caactaagtc tatctttcct aagggtgaaa aaaatccata
     5521 tggtgaattc tttattaggc aatcctatct cgcagcatta gcaaaatctc cagatgggaa
     5581 tgtttcagtt ggaaatgtaa cctttgaagc tggctgtcgt aataattggc atgttcattt
     5641 ggacggttat caaatcctcc tcgttacaga aggttcaggt tggtaccaag aagagaataa
     5701 aaaggcagtt tcattagaac caggtaacgt gattgtaact gataaaggcg ttaaacattg
     5761 gcatggtgct aaaaaagatt ctgaatttgc acatattgcg attacagctg gtaagagtga
     5821 gttttatgaa gctgttagtc ttgaagaata ttcaagacta ggttaaattc aagtaaggag
     5881 gaatcaatcc attatgaaag aaaaacaaac agccggacgt cgtcaattag aagaatttgc
     5941 acctgaattt gccagatata atgatgatat tttatttggt gaggtttggg caaaagagga
     6001 tcatttaaca gataaaacac gttctattat tactatatct gccttaatta gctgaggaaa
     6061 tcttgaacag ttagagcatc atcttcaatt tgccaaacaa aacggagtta ctaaagaaga
     6121 aattgctgac attatcactc acctcgcttt ttatgttgga tggccaaaag cttggtcagc
     6181 ctttaataaa gctaaggaaa tttggatata aaataacaga catagcaagg gagatacttt
     6241 cccaaaagcc aaaaagaaag atcaaccaat tattaaacca actattagga gaatgatatg
     6301 cttactattc aagaaatgaa agatacaacc tttgaattac aagaaaattt tagaaggtta
     6361 aattatccta ttaaacaagt tgctaaagac ttgcaactta atatcagtga agttgaatct
     6421 cttttaagtc ttgatgttac ttatcctgga gatgtttgta tgctgagaga ttatctagaa
     6481 gatatgttga aaaaagaggg taaagaagtt tatccatttt ctagattagc atctcattca
     6541 gcaaatcgtt ggtaccctta tgagacacca tggcgttatt aaagactaaa gattaatcca
     6601 tcatctttag agaaataaac aaaaatagtc aatagattct ttttagaaaa aagctataaa
     6661 ataagtgcct cttagagaa
//
LOCUS       DATRME010000095         6398 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRME010000095 DATRME010000000
VERSION     DATRME010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            203..907
                     /locus_tag="VBC23_001890"
     CDS             203..907
                     /locus_tag="VBC23_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO6843813.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            1173..2405
                     /gene="arcA"
                     /locus_tag="VBC23_001891"
     CDS             1173..2405
                     /gene="arcA"
                     /locus_tag="VBC23_001891"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6843814.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            2411..2841
                     /locus_tag="VBC23_001892"
                     /pseudo
     CDS             2411..2841
                     /locus_tag="VBC23_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2857..3870
                     /gene="argF"
                     /locus_tag="VBC23_001893"
     CDS             2857..3870
                     /gene="argF"
                     /locus_tag="VBC23_001893"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6843815.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3933..5360
                     /gene="arcD"
                     /locus_tag="VBC23_001894"
     CDS             3933..5360
                     /gene="arcD"
                     /locus_tag="VBC23_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6843816.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAIYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            5381..6337
                     /gene="arcC"
                     /locus_tag="VBC23_001895"
     CDS             5381..6337
                     /gene="arcC"
                     /locus_tag="VBC23_001895"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6843817.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
BASE COUNT         2117 a         1077 c         1180 g         2024 t
ORIGIN      
        1 tttctagacc tttctaatca ttagatgcga acattttttg tataatgaaa attttttaat
       61 gtataaaatt ttcttgtcac gataaatcct ccttcattat agcaaaaaaa ttcgtagaaa
      121 gaacgttttt cgttgaattt tgaaaacgct tttgagaaaa tgtaattgtt ctgatatctt
      181 ttaaaaaaat taagaggcct tcattactaa atttattgtt gattcatcgt actggaattt
      241 gtttccaact tctaaaattg gagtaatctt aatcaaagat tatcacatgg acaggaacct
      301 tgaaacagaa cttaaacagt tactgtctga tagtcattct ttagcaaaaa aatatttgca
      361 agagaaagaa ttcagtcaaa acaaagttat ccaaacttat cgcaaagcat atcaaacctt
      421 taaaactaaa aaaggtgcac gttcaagtat cgaagcatta ttgaagcgaa ttaattcagg
      481 aaacgaaatt actagtataa atcctctagt tgatatctat aatgccgcta gccttaggtt
      541 tggattacct attggtgctg aggatagtga tacatttaga ggtgatttaa aactaactat
      601 taccaatggt ggtgatgaat tttacttgat tggcgaagat tttaacatac caactctttc
      661 aggtgagctt gcctatgttg atgatgttgg agcagtttgc cgctgtttta attggcgtga
      721 tggaaaacgt actatgatta ccgataatac tcagaacgct tttttagtta tcgaattaat
      781 tgataataga agagaaatta tttttaaaga agcgttggac tttatcgcca ccaatacaaa
      841 tcgctttcta aaagctaaaa cacagaccat catattagat aaagaacaca gtgaaataac
      901 gctataacat taaaacgaaa agtaaaaatt ttacttttcg ttatttttta ggttaataat
      961 ttctcacttt ccaacgataa aagaaagaaa ttgtgtagaa atcgctttca tttttataaa
     1021 tttaaatttt tttggatata atcgtgacat tcttaacact tttaatgcga ataacttccc
     1081 tattttatat tcaaaagaaa gtagaatgta agtgtagata tagagataaa tcctgtttca
     1141 atgcaaatct ctaactaaga aaggagtaaa atatgactca aacacatcca attcatgttt
     1201 tctcagaaat tggaaaactt aaaaaagtta tgctacaccg tccaggtaaa gaaatagaaa
     1261 atctgatgcc tgattattta gagagactct tgtttgatga tatcccattt ttggaagatg
     1321 cacaaaaaga acacgatgcg tttgcacaag cacttcgcaa cgaaggagtc gaagtacttt
     1381 accttgaaaa tcttgctgcc gagtcgctaa caaaccaaga aattcgtgaa cagtttatcg
     1441 atgagtacat tggcgaagca aatgttcgcg gtcgtgctac taaaaaagct attcgagaat
     1501 tattactcaa tatcaaagat aataaagaac ttatcgaaaa aactatggca ggtatccaaa
     1561 agtctgagct acctgaaatc ccatcttctg aaaaaggtct aactgatctt gtcgaatcta
     1621 attatccatt tgccatagat ccgatgccaa atctttactt tacacgtgat ccatttgcaa
     1681 ctattgggaa tggagtttca ttaaaccata tgttctctga aacacgtaat cgcgaaactt
     1741 tatacggtaa atatatcttt acacatcacc cagagtacgg tggaaaagtc cctatggtct
     1801 atgatcgtga agaaacaact cgtattgaag gtggagatga gctcgtcctt tctaaagatg
     1861 tccttgccgt gggtatctct caacgtactg atgctgcctc tattgaaaaa ctcttagtca
     1921 atatttttaa acaaaattta ggttttaaaa aggttttagc atttgaattt gcaaataatc
     1981 gtaaatttat gcaccttgac acagtcttta caatggttga ctatgacaag tttacaattc
     2041 acccagaaat tgaaggcgat cttcgcgttt actctgtaac ttacgaaaac caagatcttc
     2101 atattgaaga agaaaaaggt gatttggcag atcttctagc gaaaaaccta ggagttgaga
     2161 aagtggaatt gattcgttgc gggggtgaca acttagtcgc tgctggacgt gaacaatgga
     2221 atgatggttc aaatacatta actatagcac caggtgtcgt tgtcgtttac aacagaaata
     2281 caatcacaaa tgcaatctta gagtccaaag gattgaaatt aattaagatt aatggaagtg
     2341 aattagttcg tgggcgtggt ggaccacgct gcatgtcaat gccatttgaa cgtgaagacc
     2401 tttaaaattt atgaagctat tagtcaatca cttgccttac caaagagctg caagcctata
     2461 tattcgcttt tctgttttcg ttatcgaaag aaatataaaa atgaaagaag aattcgatga
     2521 taatgacgag caagatacta tttatgctgt tctttatgat ggaaacagcc agtttcaaca
     2581 ggacgctttc ttcctgaaac tcaaacagaa gctcgtttga ctcgaattgc gactttaaaa
     2641 ggttatcgtg gtaacggcta tggtaccaaa attattatag cactagaaaa ttatgcaaaa
     2701 gaaaatggtt atcactatct aactatacat gctgaattga cagcaaaaga tttctaccag
     2761 acacttgggt atcaagctac tgggaatatt tatatggaag acggggaagc ctgtcaaaca
     2821 ctcgaaaaat atttaattta aaggagacat taacatatga cacaagtatt tcaaggacgt
     2881 agtttcttag cagaaaaaga tttttctcgt gaggaatttg aatatcttat tgatttttca
     2941 gctcatttaa aagaccttaa aaaacgtggt gttcctcatc attatcttga aggtaaaaat
     3001 attgctctct tatttgaaaa aacatctact cgtactcgcg cagcctttac aactgcagca
     3061 attgacctag gcgctcatcc ggaatacctt ggtgcaaatg atattcaact tggtaaaaaa
     3121 gaatcaacag aagatactgc taaggtttta ggacgtatgt ttgatggtat tgaattccgt
     3181 ggttttagcc aaagaatggt tgaagagctt gctgaatttt ctggagtacc tgtctggaat
     3241 ggtttaacag atgaatggca tccaacacaa atgctagctg actaccttac tatcaaagaa
     3301 aacttcggta aacttgaagg tattactctt gtttactgtg gtgacggacg taacaatgtt
     3361 gccaactcgc ttttagtggc tgggactttg atgggggtca atgtacacat cttttctcca
     3421 aaagaacttt tccctgctga agagattgtt aaattggctg aaggatatgc caaagaatct
     3481 ggggctcacg ttctcgttac tgataatgta gacgaagctg taaagggagc agacgtcttt
     3541 tacactgatg tctgggtatc gatgggagaa gaagataagt tcaaagaacg cgttgaactt
     3601 cttcaaccat atcaagtaaa catggaactg attaaaaaag ctaataatga taatcttatc
     3661 ttcttacact gcttacctgc attccatgat acaaataccg tttatggcaa agacgtcgct
     3721 gaaaaatttg gggtcaagga aatggaagtt actgatgaag tcttccgtag caaatatgct
     3781 cgtcatttcg accaagctga aaatcgtatg cacactatta aagctgtaat ggctgcaacc
     3841 cttggaaatc ttttcattcc aaaagtttaa attaaacgac tatagggagt tgacatatag
     3901 tcaattccct attttaggaa atgaggtata aaatggaaaa agaaaaaaaa ttaggtcttt
     3961 taccactaac aatgcttgtc attggctctc ttatcggtgg cggaatcttt gatttaatgc
     4021 aaaatatgag ttccagagcc ggtttggtac caatgcttat tgcttgggta attactgcta
     4081 tcgggatggg aactttcgtt ttaagttttc aaaatttatc tgaaaaaagg ccggatctaa
     4141 cagctggaat ctttagttac gctaaagagg ggtttggaaa ctttatggga tttaactctg
     4201 catggggtta ttggttatca gcttggcttg gaaatgttgc ctacgctgca ctcttattca
     4261 gttcactcgg ttatttcttt aaattctttg gtaatggaaa taatatcatc tcaattattg
     4321 gagcaagtat agttatttgg gttgtccatt tcttaatttt aagaggtgtt aatacagctg
     4381 catttattaa taccgtagtt acctttgcaa aattagtacc tgttattatt ttcttaattt
     4441 cagcgttatt agctttcaaa tttaacattt ttagtcttga tatctgggga aatggattac
     4501 atcaatcaat tttcaaccaa gtcaattcaa ctatgaaaac cgctgtttgg gtatttattg
     4561 gtattgaggg cgccgttgtc ttctcaggtc gtgctaaaaa acactctgat attggtaaag
     4621 caagtatcct agcattattc actatgattt cactttatgt attgatttct gttttatcac
     4681 ttggtatcat gtcacgtcca gaacttgcaa acttaaaaac accagctatg gcttacgttc
     4741 tagaaaaagc tgttggtcac tggggtgcta tcttagttaa ccttggtgtt atcatttcag
     4801 tatttggcgc tattcttgct tggactttat ttgcagcaga attaccatat caagctgcta
     4861 aagaaggtgc ttttcctaaa ttttttgcaa aagaaaataa aaacaaagct ccaatcaact
     4921 cactcttagt cactaatctt tgtgtacaag cattcttaat cacgttctta ttcacacaaa
     4981 gtgcttatcg ttttggtttc gcattagcat catctgctat cttaattcct tatgctttta
     5041 cagcactata tcaattacaa ttcacactcc gtgaggataa gtcaactcca ggacatcaaa
     5101 agaatttaat tatcggtatc ctcgctacaa tctatgctat ttaccttatc tacgctggtg
     5161 gttttgatta cttacttttg acaatgattg cttatactct aggtatgatt ctctatatta
     5221 aaatgagaaa agatgacaag cttcctatct ttgtgagata tgaaaaaatt agtgccattg
     5281 tcattctagc gctttgctta ctatgtatta ttgaaattat gactggacaa attgacattg
     5341 ctgcaatcct agcaggttag tcattagaaa gagggataaa ttggtaaaag aacatcaaaa
     5401 aatcgtagtt gccttaggtg gtaacgctat tctatcaaca gatgcttcag caaaagcaca
     5461 acaagaagca ttaatcaaca catcaaaatc actggtcaaa ctgattaaag aaggccatga
     5521 tgtcattgtt actcatggaa atggtcccca agttggtaac ttattgctac agcaagcaga
     5581 ttcagattct gaaaagaatc cagcaatgcc tttggatact tgtgttgcca tgacagaagg
     5641 aagtattggt ttttggttac aaaatgctct taacaatgaa ttacaagaac aaggcattga
     5701 caaagaggtt gcaacagtgg taactcaagt tattgtagat gaaaaagatc aagctttcac
     5761 taatccaaca aaacctattg gcccattctt atctgaagaa gatgccaaga aacaagccca
     5821 agaaacaggt tcaaaattta aagaagatgc tggtcgtgga tggcgtaaag tcgttccttc
     5881 accaaaaccg gttggtatca aagaagcttc tgttattaga cggttagtgg actctggtgt
     5941 ggttgttatt agcgcaggtg gcggaggtgt accagttatt gaagatgcta atacaaaagc
     6001 attaaaaggg gttgaagctg ttattgacaa agactttgcc agtcaaactt tatcagaact
     6061 tgttgatgcc gacttattca tcgtcttaac tggcgttgac aacgtttttg ttaattttaa
     6121 taaacctaat caagaaaaat tagaggaagt tactgtttca caaatgaaac aatatatcac
     6181 tgaaaatcaa tttgcccctg gcagtatgct accaaaggta gaagctgcga ttgcttttgt
     6241 tgaaaataaa ccagaagcgc gtgctatcat tacttcactt gaaaatattg ataatgtctt
     6301 agcagaaaat gcagggacac aaattgttgt aaattaaaca atgtagatta ccattacata
     6361 cactccttat taataacaaa gtaatgtcac tccttagg
//
LOCUS       DATRME010000096         6338 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRME010000096 DATRME010000000
VERSION     DATRME010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..146)
                     /locus_tag="VBC23_001896"
     CDS             complement(<1..146)
                     /locus_tag="VBC23_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6843818.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQL"
     gene            349..2388
                     /locus_tag="VBC23_001897"
     CDS             349..2388
                     /locus_tag="VBC23_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6843819.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSIYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGMMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2498..3076
                     /locus_tag="VBC23_001898"
     CDS             2498..3076
                     /locus_tag="VBC23_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6843820.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(3110..4351)
                     /locus_tag="VBC23_001899"
     CDS             complement(3110..4351)
                     /locus_tag="VBC23_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6843821.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            4529..4657
                     /locus_tag="VBC23_001900"
     CDS             4529..4657
                     /locus_tag="VBC23_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6843822.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            4718..6031
                     /gene="obgE"
                     /locus_tag="VBC23_001901"
     CDS             4718..6031
                     /gene="obgE"
                     /locus_tag="VBC23_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6843823.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            6057..6215
                     /locus_tag="VBC23_001902"
     CDS             6057..6215
                     /locus_tag="VBC23_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843824.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT         2081 a         1058 c         1266 g         1933 t
ORIGIN      
        1 agttgcttat ttcgtttgac ttcttttgcg atagtagtag ggtgtttcag tagattaagc
       61 ccgatggctt taaaggtttc tcctctatcc aaaccagatt ggatgtcatt acggtctgag
      121 agagttaggt gtttgtgttt tgtcatgatg gataaactca tttctaaccc aaacgtctca
      181 gacttaattc caccataaaa atccttttca cactaacttc cggttttgga gggcagacgg
      241 aacttacttt gagaaattac ttaatataaa ataacagtta atattttagc aatttgataa
      301 cacttagatt ataatgtaaa cgtatacttt tttagattgg aagatgaaat gaaacgtaaa
      361 tactttgttc ttaatacggt gacggtttta acgttagcta ctgcaatgaa tactagcagt
      421 atctatgcta atagtactga gacaagtgct tcagtagctc ctactacaaa tactatcgtt
      481 caaactaatg acagtaatcc taccgcaaaa tttgcatcag aatcaggaca atctgtaata
      541 ggtcaagtaa aaccagctaa ttctgcggcg cttacaacag ttgacacgcc tcatatttca
      601 gctccagatg ctttaaaaac aactcaatca agtcctgtcg ttgagagtcc ttctactaag
      661 ttaactgaag agacatacaa acaaaaagat ggtcaagatt tagccaacat ggtgagaagt
      721 ggtcaagtta ctagtgagga actcgtcaat atggcatacg atattatcgc taaagaaaac
      781 ccatctttaa atgcagtcat tactactaga cgccaagaag ccattgaaga ggctagaaaa
      841 cttaaagata ctaatcagcc gtttttaggt gttcccttgt tagtcaaggg gttagggcac
      901 agtattaaag gtggtgaaac caataatggc ttgatctatg cagatggaaa aattagcaca
      961 tttgacagta gctatgtcaa aaaatataaa gatttaggat ttattatttt aggacaaacg
     1021 aattttccag agtatgggtg gcgtaatata acagactcta aattatacgg tccaacgcat
     1081 aatccttgga atcttgctca taacgctggt ggctcttctg gtggaagtgc agcagctatt
     1141 gctagcggaa tgacgccaat tgctagcggc agtgatgctg gtggttctat ccgtattcca
     1201 tcttcttgga cgggcttagt aggtttaaaa ccaacaagag gattggtgag taatgaaaag
     1261 ccagattcgt atagtacagc agttcatttt ccattaacta agtcatctag agacgcagaa
     1321 acattgttaa cttacctaaa gaaaagcgat caaacgctag tatcagttaa tgatttaaaa
     1381 tctttaccaa ttgcttatac tttgaaatca ccaatgggaa cagaagttag tcaagatgct
     1441 aaaaatgcta ttatggacaa cgtcacattc ttaagaaaac aaggattcaa agtgacagag
     1501 atagatttac caattgatgg tagagcatta atgcgtgatt attcaacctt ggctattggc
     1561 atgggaggag ctttttcaac aattgaaaaa gacttaaaaa aacatggttt tactaaagaa
     1621 gacgttgatc ccattacttg ggcagttcat gttatttatc aaaattcaga taaggctgaa
     1681 cttaagaaat ctattgtgga agcccaaaaa catatggatg attatcgtaa ggcaatggag
     1741 aagcttcaca agcaatttcc tattttctta tcgccaacga ccgcaagttt agcccctcta
     1801 aatacagatc catatgtaac agagaaagat aaaagagcga tttataatat ggaaaacttg
     1861 agccaagaag aaagaattgc tctctttaat cgccagtggg agcctatgtt gcgtagaaca
     1921 ccttttacac caattgctaa tatgacagga ctcccagcta tcagtatccc gacttactta
     1981 tctgagtctg gtttacccat agggatgatg ttaatggcag gtgcaaacta tgatatggta
     2041 ttaattaaat ttgcaacttt ctttgaaaaa catcatggtt ttaatgttaa atggcaaaga
     2101 ataatagata aagaagtgaa accatctgct gacctaatac agcctactaa ctccctcttt
     2161 aaagctcatt catcattagt aaatttagaa gaaaattcac aagttactca agtatctatc
     2221 tctaaaaaat ggatgaaatc gtctgttaaa aataaaccat ccgtaatggc atatcaaaaa
     2281 gcacttccta aaacaggtga tacagaatca agtctatctc cagttttagt agtaaccctt
     2341 ttattagctt gttttagctt tgtaacaaaa aagaatcaga aaagttgatt gtaatgtaaa
     2401 ataattccta tttttcactt tttagtatca tattttcgat ttttatgttg taataaatta
     2461 taatgaagat gtaaggaatt ataaaatagg aaataatatg aaagtgaaaa ataagatttt
     2521 aacgatggta gcacttactg tcttaacatg tgctacttat tcatcaatcg gttatgctga
     2581 tacaagtgat aagaatactg acacgagtgt cgtgactacg accttatctg aggagaaaag
     2641 atcagatgaa ctagaccagt ctagtactgg ttcttcttct gaaaatgaat caagttcatc
     2701 aagtgaacca gaaacaaatc cctcaactaa tccacctaca acagaaccat cgcaaccctc
     2761 acctagtgaa gagaacaagc ctgatgggag cacgaagaca gaaattggca ataataagga
     2821 tatttctagt ggaacaaaag tattaatttc agaagatagt attaagaatt ttagtaaagc
     2881 aagtagtgat caagaagaag tggatcgcga tgaatcatca tcttcaaaag caaatgatga
     2941 gaaaaaaggc cacagtaagc ctaaaaagga acttcctaaa acaggagata gccactcaga
     3001 tactgtaata gcatctacgg gagggattat tctgttatca ttaagtcttt acaataagaa
     3061 aatgaaactt tattaaatat aaaaaggagg actaacctcc tttttatatt taaatagccc
     3121 attcgccatt tctaaagatt gggacagcag ttccatctgc acgaatacca tcaatatcca
     3181 tttgttctga accaatcata aaatcaacat gtgttgaaga acgatttaaa ccagcttcat
     3241 ccaattcttg acttgtcatt tctgttcctc cttctacatt gaaggcatat gcagatccaa
     3301 tagcgaggtg attagaggca ttttcatcga ataaagtatt aaagaaaatt agtccagaca
     3361 gtgaaattgg tgttttatgt gggacaagtg caacttcccc aagcgatctt gccccatcat
     3421 tttcctcgat caagcgttgg attgtttctt gccctttttc tgcagtgaca ttgataattt
     3481 taccgtcttt aaaagtaaac gtcatatttt cgataataac gccagcataa ctgagaggtt
     3541 ttgtacttgt cacataccca tctgcacgac ggtaatcagg tgctgaaaag atttcttcag
     3601 taggcatatt agcgataaaa gtctctccct gagcgttgag actaccggct gcctcccaaa
     3661 tgtgattttt aggcatacca agcgttaaat ctgtacctgg tgcggtataa tgaagagcat
     3721 caaattgttc ttgatttagt ctagtagctt tttctaataa tttagcttca tgtgcatccc
     3781 aagcagcaat aggattatct tcataaatac gatttaattt gaaaatggta tcccaaagag
     3841 catcaacttg ttggtcgctt gtttttaatt ctgggaagac aagtgcagcc cattctctac
     3901 cagcagctgc agccacgttc caagatactt tattagcttg tattgctcta ctttgttcct
     3961 ctaaggcaat agcggttgct ctagttgttt tagataaacg tttaggatca acagtagcga
     4021 aagcgttagg gttagaagat ttcacaacta atcgactagc cttcttattt aatagcgcta
     4081 aagatttatc aacggtatac tgtggaacat tcgtgagaac ttcgtctgaa gcatgaagta
     4141 gtcgctggcg cgtgataaaa tcatctgtat aatcaacaat aacttcactt gctccctttt
     4201 cataagcgac ttcagttaaa agccttgcta agtggacttg ttccacatcg attgttaaag
     4261 cgagagtgtg ccctttttgg acatttaaac ctttagaaat aattaattgg gcatattttt
     4321 ttaaaaggtt gtcgaaatct tgtaaaacca tactagctcc tttaaattta ctatttttta
     4381 taatttatca taaactctta taaagcacaa ggaaatatgg aagcgattta ataaagatat
     4441 atttaagcta acggtttgta gtttgcgaaa taagacgttt cgtgttaata tagacttaac
     4501 tatatattca gtaaatgagg tgacatgagt ggcttttgga gataacggac aacgaaaaaa
     4561 aactgggttt gaaaaactaa ctctttttgt tgttattttg atggtactag taacagttgg
     4621 tggtttggta tttggtgcta ttagtgcaat catgtaatga gaaacgctgt aaagcgtttt
     4681 tttatactga atagaataaa gaataggaat tttagaaatg agtatgtttt tagatactgc
     4741 caaaattagt gttaaggcag gtcgcggtgg tgatggcatg gttgcttttc gccgtgagaa
     4801 atatgtcccc aatggaggcc cgtggggcgg tgatggcggt aaaggcggat cggtcatttt
     4861 taaagtaaac gaaggtttac gaactttgat ggattttcga tataaccgta attttaaagc
     4921 aaaagctggt gaaaaaggga tgactaaagg tatgcacggt cgaggtgcag aagaccttat
     4981 tgtgtcatta ccacctggaa caactgttcg tgatgctaat acaggtaagg ttattaccga
     5041 ccttgtggag catgatcagg aattcgttgt agccagaggt ggccgcggtg gtcgtggaaa
     5101 tattcgcttt gcaaccccac gtaatccagc tcctgaaatt gctgaaaatg gggagcctgg
     5161 cgaagaacgt gaattacaat tagaattaaa aattttggct gatgttggtc tcgttggttt
     5221 cccatcagtt gggaagtcga cgctacttag tgttgtttca gcagcaaagc ctaaaattgg
     5281 tgcctatcat tttacgacaa ttgtccctaa tttagggatg gttcgtacaa aatcaggtga
     5341 tagctttgca atggctgatc tccctggttt gattgaaggg gctagtcaag gtgtcggatt
     5401 aggaactcag ttccttagac atattgagcg tacacgtgtt attctacatg ttattgatat
     5461 gtcggcaagt gaaggacgag acccttacga tgactatatt tcaattaata atgagttgga
     5521 aacttacaat cttcgattga tggaacgtcc gcaaattatt gttgccaata aaatggatat
     5581 gcctgattca gaagagaatt tggcagcatt caaagaaaaa ttagctgcaa actatgatga
     5641 gtttgatgac atgcctatga tttttccaat ttcaagttta gcacatcaag gactagagaa
     5701 ccttatggat gcgacggcta aattattagc caatacagaa gagtttcttc tttatgacga
     5761 aactgacatg caagaagatg aagcatacta tggatttaac gaagatgaac gtccatttga
     5821 gattacacgt gatgatgatg caacgtgggt attgtatggt gataagctag aaaaactctt
     5881 tgttatgaca aatatggaac gcgatgaatc aataatgaaa tttgctcgtc agttacgtgg
     5941 tatgggcgtt gatgaagcac ttcgagagcg cggagctaag gatggagata ttgttcgtat
     6001 aggtaacttc gaatttgaat ttgtagatta atgcagtact tgattggagg ttttttatgg
     6061 gagataaacc gatatcattt cgtgataaag atgggaactt tgtttcagcg gccgatgtct
     6121 ggaatgctga aaaattagaa gaactcttta acacattaaa tcctaatcgt aaacttcgtt
     6181 tagaacgcga aaaattagca aaagaaaaag gatagttaag taactatcct ttttagttat
     6241 tttgggtatg aaaaaagact ctaaaacata attgatagag tgttaaggta cagattactt
     6301 tattatttta aacgtttttg agctttttga tatactga
//
LOCUS       DATRME010000097         6123 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRME010000097 DATRME010000000
VERSION     DATRME010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            46..1583
                     /locus_tag="VBC23_001903"
                     /pseudo
     CDS             46..1583
                     /locus_tag="VBC23_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1760..3304
                     /locus_tag="VBC23_001904"
     CDS             1760..3304
                     /locus_tag="VBC23_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6843825.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3390..3770
                     /locus_tag="VBC23_001905"
     CDS             3390..3770
                     /locus_tag="VBC23_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6843826.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            3964..4698
                     /locus_tag="VBC23_001906"
     CDS             3964..4698
                     /locus_tag="VBC23_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6843827.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            4691..5353
                     /locus_tag="VBC23_001907"
     CDS             4691..5353
                     /locus_tag="VBC23_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843828.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            5369..>6123
                     /locus_tag="VBC23_001908"
     CDS             5369..>6123
                     /locus_tag="VBC23_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843829.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVL"
BASE COUNT         2031 a          918 c         1195 g         1979 t
ORIGIN      
        1 ggttaattat tttttgaaat atattttttc tggaggaaaa tagtaatgaa taaatcattc
       61 aataccaaat taggtttggt aactgtagcg gcattatcag gtattgtatt aacgtctcaa
      121 ttacctgtga acgctaaagc tgagacacca gtaatggcag caagtgcaca acaaggtttc
      181 cgttttgtag caaccgttgt agatagtcag acgcgtgtct tgccgggaaa attggtaact
      241 ttgtcagagg tgacaactgg tcagcctaaa attattgcaa gtgtaaaaac gaatgatgcc
      301 ggacaagcta tttttaataa tctccctata aaacgtaacc ttagtgtgtc tgttgatgga
      361 caagtaaaag gatatactat tcgaactgac gtagcaggct ctagtaaagc agctagtttt
      421 acagcaacag gagtagggac aaatgagcca acgtattcaa aaaaaaccat agacattaca
      481 gtgagagacc aaaatgctga acctgtttca ggcaggacag ttaccttaaa aacacatgct
      541 ggtcgtgaga ttgctagctt ggtttctggt gacaatggat taactcgctt tactgatcgt
      601 ttactagatg gaacattcta ccaatatttt gttgatggga aaaaattggt gatattgtac
      661 ctggagaatc aagaagtgca tatgttaatg ttgcacctaa aaaagattat tttaccttta
      721 cagtcactgc tttagataaa gatggacttg ttgttaaggg aaaagaagta acgttgactg
      781 atattactga tggtaaagca gtagcgcttg cttctttaaa aacgaatgat aatggacaag
      841 ctatctttac caacctacca ttatcaagaa atatcagtgt ttcaattgat ggaaaatcaa
      901 agggctatac tcttcgaact gatgtagcag gctctcataa agcggctgca ttctatgtag
      961 atggtaaagg taccaaagca cctcaattta gtgcagaagc agcggttgta actatttacg
     1021 atgctaatgg aaatactata gcaaaccaag aagtgacatt aactaattca aatgggacaa
     1081 ttgttgcaaa aggtttgact gaaaaagatg gcaaagcaag gttcgccaat aaattaatgg
     1141 gaggaacgtt atacaatatt tttgtaaatg gtattgagat gcctaaaact gcccttgttg
     1201 gtagtgatgt cagtgttttt ctaacagata aacaaattaa gaaagaaaaa cctgttactc
     1261 ctgataaatt ggataaagaa atgaaccaag agaaggaaaa ggagctctta gatactccaa
     1321 aaccagtacc acctacttcg aaaccaacag caccaattaa aaataaaaag gacgattccc
     1381 ctaagaagtt tacgaaaagt caaccttcta gtttgaagtc aagtgttaag gctataggtg
     1441 ctaagccaaa agctatgaca gccttagcta aaaaattgcc taaaactggt gatcaagcca
     1501 ttagtatttt aactttggtt ggttttattg taacagggtt agcgattatg ttaggcttct
     1561 ttaaaaaatc acgatcaaaa taatttattt tagaagcttg ggtctttaga cacaggcttc
     1621 tcttctttct tcggaaatag attgaacttg aagaatgttg ttaaattcgc tataataata
     1681 aagctataga caagattatt gtttcagttg agactactga aaataatact aagaaatgga
     1741 taaaagctaa ggattgaata tgacattaca agacgaaatc aaaaaacgcc gtacttttgc
     1801 catcatctct cacccggatg ctggtaagac gactattact gagcaattat tatattttgg
     1861 tggtgaaatt agagaagcag ggacagtaaa agggaaaaaa tcaggtactt ttgcaaagtc
     1921 cgactggatg gatattgaaa agcaacgggg tatctctgtt acttcatctg ttatgcaatt
     1981 tgattacgcg ggtaaacgtg ttaatatttt ggatacacct ggacatgagg atttctcgga
     2041 agatacatac cggaccttaa tggctgtgga tgcagctgtc atggttgtag attcggctaa
     2101 aggtattgag gctcaaacta aaaaactttt tgaagttgtt aagcatcgta atattccggt
     2161 ttttacgttt attaataaat tggaccgtga tggacgtgag ccactggatt tgcttgaaga
     2221 gttagaagaa gtactaggta ttgcttctta tccaatgaat tggccaattg gtatggggaa
     2281 atcatttgaa gggctttatg atttacataa taaacgttta gaactctata aaggagacga
     2341 gcgttttgca agtattgaag acggtgatca gctgtttgcc aataatccat tttatgaaca
     2401 agtcaaagaa gatattgaac ttcttcaaga agcaggtaat gacttttcag aacaagctat
     2461 tcttgatggt gacctaactc cagtattctt tggctctgcc cttacgaact ttggagttca
     2521 gacttttctt gatactttct tggagtttgc tccagaacct catggtcaca agactacaga
     2581 aggtaatgtt attgacccct tagctaaaga tttttcaggt tttgtcttta aaattcaagc
     2641 taacatggac ccacgtcatc gcgaccgtat tgcttttgtt cgtattgttt ctggtgaatt
     2701 tgagcgcggc atgggagtta atttgacacg cactggtaaa ggcgctaagc tctccaatgt
     2761 tactcaattt atggcagaat cacgagaaaa tgtgacgaat gctgttgctg gggatattat
     2821 cggggtttat gatacaggaa cttatcaagt tggggatacc ttaacggttg ggaaaaataa
     2881 atttgaattt gaaccattac caacttttac cccagaatta tttatgaaag tatcagcaaa
     2941 aaatgttatg aagcagaaat cattccacaa gggtattgag caattagtac aagaaggagc
     3001 aatccagctt tataagaatt atcagacagg cgaatacatg ctgggtgctg tgggtcaact
     3061 tcagtttgaa gtttttaaac atcgtatgga aggtgaatac aatgctgaag ttgttatgac
     3121 accaatgggt aaaaagactg tacgttggat taactcagac gatttagacg aacgcatgtc
     3181 ctcaagccga aatattttag caaaagatcg ttttgaccaa ccagttttcc tatttgaaaa
     3241 tgattttgca cttcgctggt ttgctgataa gtatcctgat gtgaagcttg aagaaaaaat
     3301 gtaaaattta aagtcactca aatttgagtg actttttaag ttttttagac taaaatagtt
     3361 ataatacata gaaaagattg gagattaaca tgggaatttt ttcgggatta atgggaaatg
     3421 cttcacaagt ggataccgat aaagtcgaga atcagttatc tgatattctg attccaggtg
     3481 aacaggttga tttagcttat actttaattc gtgatttgat tgtttttact aattatcgtt
     3541 taattttagt cgataaacag ggggtgacag gtaaaaaggt ttcttataat tctatacctt
     3601 atgcttctat atcacgtttt acagtggaaa ctagtggtca ttttgattta gatgctgagt
     3661 taaagatttg gatatcatca gcaatagaac cagcagaagt acttcagttt aagaacgacc
     3721 gtaacatagt gagtatacag aaagctttgg cgatagctgt tttaaaataa acaatttagc
     3781 aagtgctgct actaagagtt atttgtttta attataactt ttaatagtgc taaattctca
     3841 ttttcaaaat aaaagtagtg atttttatca ctgcttttat tttgaagtat agtaggcctc
     3901 aagcatatat tttgaaaacg ctgtattttt ttgataagat atttttttga ggagtattaa
     3961 ataatgataa tagaattaaa gagtgttggg aaaatttttc caaaatcaca gtttagatta
     4021 gatgatattt cttttaatat agcagaaggc gagatagttg gtctgattgg ttctaatggt
     4081 actggaaaga gtactatttt gaagatgatt aatggcttaa tcccttatga taaaggaaat
     4141 atttattatc aaggaaaaga agtgaagtct ttttctgata ataagttacg tcagatgcgt
     4201 aaagatattg cttatatttt tcaaaatcat aatttgttag caggagaaag tgtatattat
     4261 catctagctt tagtttataa attaaatcat caaaaagtaa atcatgacgc aattaatgat
     4321 atcttagatt ttttaggttt gatggattta aaacaggtga aatgccatag tttgagtggt
     4381 gggcagcaac aaaaagttgc aatcgctatg gctgttttgc aaaaaccgaa acttattcta
     4441 tgtgatgaga tcagctctgc cttagatact aatagtgaaa aagagatatt taacttgttg
     4501 tctgatcttc gtgaaaaata tggtatctct atcttgatga ttgctcatca cctatctctt
     4561 ttaaaacaat attgtgatcg tgtaatgatt ttggatcacc agactattgt agatactgtt
     4621 gttccagtga aagcaactct gaatcaatta aagtcaaact acgtagatca agtaaaggag
     4681 tttttgttac atgattgaca ttattgaaaa acatggcgaa ggtatcttaa aatcattttg
     4741 ggaaaccaat ttaatgttag ggttaacctt gattctttgt tttctaattg cttttccaac
     4801 aggtatttta ttgttttcat tgagaaagtc ttacttgatt aaacattctt tagcctatca
     4861 actactaaat ttatttttag gtacgttacg gagtgtccct ttccttattt ttatttttat
     4921 cttaatccct ctcaatcgat taatttttgg gacctcattt ggaacgatag cggcaatctt
     4981 acctcttact ttggtttcag tcagtttgta tgcacgttat gtggaacaag ctttattaaa
     5041 tatacctcaa gttgttgtag atcgagctct cagtctaggg gctaacaaga gacagataat
     5101 ttattatttt ttaatcccct ccatcaagat tgaccttgtt ttatcattta ctgcaacggc
     5161 tataagtatt cttggctact cgacaataat gggagttatt ggtgcaggag gtttgggtga
     5221 atatgcctat cgttttggct atcaagagta tgactaccca gttatgtact tgattgttgt
     5281 cttatttata atatatgtgt ttatcttaca gagtttgggc tattttatag caaaccgcta
     5341 ctcaagaaaa taaaagaaaa gaggaactat gaaaaaatta ctttccctaa catgtctaat
     5401 catgatgtct ttatgtttag tggcatgtac taagcaagca atgtcgtcta agcaaattaa
     5461 agataagaat agtaaagaaa aggtgattac tgttgcaact tacagcaaac ctacatctac
     5521 ctttttagat ttgattaaag ataatgtaaa agaaaaagga tatactttaa aggttgtcat
     5581 ggtctctgac tatattcagg ctaacattgc tttagaaaac aaagaacatg atgctaacct
     5641 tttacaacat gaatttttca tgagtatctt taataaggaa aatgatggtc atctagtgtc
     5701 aattacacca atttatcatt cattggctgg tttttatggt caacatttga aaaatattgc
     5761 cgagcttaaa gacggtgcta aggtagcgat tccgtctgat cctgccaata tgactagagc
     5821 tctgctatta ttgcaagaaa agaaacttat caccttaaag aatacgtcca aaaagaccaa
     5881 ggctatcgaa gatattatta ctaaccctaa aaaattacga attgaacctg tagcattact
     5941 taacctcaat caggcctatt ttgaatatga ccttgtcttt aatttccctg gatatgtgac
     6001 aaaaatcaat ctagttccta aaagggatag attattatat gagaaaaaac cagatatccg
     6061 ttttgcaggt gccttggtag ctcgtgaaga taataaaaat agtgataaaa taaaagtact
     6121 taa
//
LOCUS       DATRME010000098         6112 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRME010000098 DATRME010000000
VERSION     DATRME010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            1..503
                     /locus_tag="VBC23_001909"
                     /pseudo
     CDS             1..503
                     /locus_tag="VBC23_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584204.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            611..1945
                     /locus_tag="VBC23_001910"
     CDS             611..1945
                     /locus_tag="VBC23_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO6843830.1"
                     /translation="MEVKKFSEKDFVNEINKIKQKQFLSQIEQYESYIAPQMRTKGYK
                     RINQSERTVVFSFGEITFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLAT
                     MMPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLY
                     VEGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVSTKRYELRDKHEILQLNYDKAKYN
                     LLDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLS
                     SYLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFK
                     YIKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILERANGLRE
                     LFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
     gene            2599..6051
                     /gene="scpA/B"
                     /locus_tag="VBC23_001911"
     CDS             2599..6051
                     /gene="scpA/B"
                     /locus_tag="VBC23_001911"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO6843831.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGILSGNAPSEMKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNP
                     PKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QVTVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQK
                     ETKK"
BASE COUNT         2221 a         1075 c         1179 g         1637 t
ORIGIN      
        1 aagcagtaat acttctaaaa agaataagac aaatcctagt ggtgcgacaa gtgcaaaagt
       61 cgttaaacac aaaaaaactg atgaagaacg agcagaagat tatataaaaa cgttaggatt
      121 aaacccgagt gattatataa aagaaatgga ttattcaaag gttgatttta gggggggatt
      181 tatcatcagt atcaggtaat tataataatt ttactgttga aaacatcaat caaacttttg
      241 aaaaaggtga taaatcttta aaagatatta ttgaaatttt tggaaagcct gatacaattt
      301 gggttaatta cgttgacaat cagtacaata ctgatttgta ctggaaaaaa gatgacacag
      361 atgattctaa ttatattcat gcagaatggt ctgagccaaa agatgaatat gatgttcaaa
      421 gtttaagtgg cacggcttca aattcaggta ctggcaagtt tcagtcattc attggattag
      481 atggatttga gcctgcacag taggataaat agaaagatca cgttattaaa tgtggtcttt
      541 tactttaact tttcgaataa aaaatagcaa taatattcta atcctgtaac aaaaattgta
      601 aaatattatc atggaagtta aaaaattctc ggaaaaagat tttgtaaatg aaataaataa
      661 aataaaacag aaacaatttt taagtcaaat tgaacagtat gaaagctata tcgctcctca
      721 aatgagaacg aaaggctata agaggatcaa tcagtctgaa cgtacggttg ttttctcttt
      781 tggagaaata acatttagta ggagtcgctg gacaaatggc tttgaaacta gaataccagt
      841 agatgagtgg ttaggtcttg aaaaatataa gagatattca atagaattct tatatcatgt
      901 tgcaaaattg gctacaatga tgccttatcg tcaagtttgc aaagtaatag atagcacttt
      961 gcaaacaatc ataacaaaag actgtgtttt aaaagcagta aaatttgtag aaaaattgtt
     1021 aaaagaaaaa gaacgctatc gtttttattt ggaagagcca cccgaacgta aaaaagtgaa
     1081 aaaactgtat gttgagggtg atggagtcat gattaaaagc acagattcta gagaggaaag
     1141 aaggtattta gatttaacac attttgttat tcatacaggc tcaaaaaaag tttctactaa
     1201 aagatatgaa ttgcgggaca agcacgaaat attacagctt aattatgata aagctaaata
     1261 taatctttta gattatattt ataataacta tgaagtagat gacgatacta ttttaatcac
     1321 taactctgat atgggtaaag gctatactag tagagttttt aaggaattag gaaaagcact
     1381 taaggtaaag aaacatgagc atttttggga tatctatcat gttaaagaaa agttaagttc
     1441 ataccttaga aaatatccaa ttgaattaac cgattttgct ttagatgcgg taaaaaaata
     1501 taattctgat aagcttgaat tagtttttga tactgttgaa tcactgattt gtgatgaact
     1561 tgaagatcaa gaatttcaga agtttaagaa aaaagtatta aataatttca aatatataaa
     1621 accagctcat cttagaaatc tttcaaatcg tggtattggt atcatggaat cacaacacag
     1681 aaagataacg tatagaatga agcgacgtgg catgtattgg tcaaagtggg gaatctccac
     1741 aatggcaaat atgattatac ttgaaagagc taacggttta cgagaattat ttttcggttc
     1801 ttggagaaag gtatacagtg agtataaaga aggttcattt agtgcagggc gactttttaa
     1861 aaagacagat gaattagata aattttctaa gccccttcta aaaaatggca gaaaatggag
     1921 tataacagga atcaaaacaa aatagaaaaa gtctaaagat acctttattt ctagcgtttt
     1981 tttgcatgtt ttaacttgca ttaaatcttc taaagtagta taattatatt aaagaaaagc
     2041 aaaaaaggtg aaggagcgcg aactcctaca ccaaaccgat taagacaaat accacttgct
     2101 taatcagtcg aaccttattg tagcataact aaaacaatac cgtcaagact gaacaagcaa
     2161 aaaatacttg ttttggtctt tttaagaata attcaagttt tcttaaattt atgaggtatc
     2221 gactaatctc aaaatttcag acacatacct gagtgaacat caagagagaa ccgatcggtt
     2281 ctctcttgat gtctaaaaga atgaggttaa ggaggtcaca aactaaacaa ctcttaaaaa
     2341 gatgaccttt actaataatc gtctttgttt tataataaag atgttagtaa tataattgat
     2401 aaatgagata catttaatca ttatggcaaa agcaagaaaa atagctgtgt catatgcaaa
     2461 taacccctgt ttgctcttta aaaaagacgt tatccttatt tctctacaca cagacggata
     2521 gctaggagag aatcgtttga ttctctcttt tcttaatggt cataaagaca aagtctctca
     2581 tcatgaaagg acgacacatt gcgtaaaaaa caaaaactac catttgataa acttgccatt
     2641 gcgcttatat ctacgagcat cttgctcaat gcacaatcag acattaaagc aaatactgtg
     2701 acagaagaca ctcctgctac cgaacaagcc gtagaacccc cacaaccaat agcagtttct
     2761 gaggaatcac gatcatcaaa ggaaactaaa acctcacaaa ctcctagtga tgtaggagaa
     2821 acagtagcag atgacgctaa tgatctagcc cctcaagctc ctgctaaaac tgctgataca
     2881 ccagcaacct caaaagcgac tattagggat ttgaacgacc cttctcatgt caaaaccctg
     2941 caggaaaaag caggcaaggg agctgggacc gttgttgcag tgattgatgc tggttttgat
     3001 aaaaatcatg aagcgtggcg cttaacagac aaaactaaag cacgttacca atcaaaagaa
     3061 aatcttgaaa aagctaaaaa agagcacggt attacctatg gcgagtgggt caatgataag
     3121 gttgcttatt accacgacta tagtaaagat ggtaaaaacg ctgttgatca agaacacggc
     3181 acacacgtgt cagggatctt gtcaggaaat gctccatctg aaatgaaaga accttaccgc
     3241 ctagaaggtg cgatgcctga ggctcaattg cttttgatgc gtgtcgaaat tgtaaatgga
     3301 ctagcagact atgctcgtaa ctacgctcaa gctatcagag atgctgtcaa cttgggagct
     3361 aaggtgatta atatgagctt tggtaatgct gcactagctt acgccaacct tccagacgaa
     3421 accaaaaaag cctttgacta tgccaaatca aaaggtgtta gcattgtgac ctcagctggt
     3481 aatgatagta gctttggggg caagccccgt ctacctctag cagatcatcc tgattatggg
     3541 gtggttggga cacctgcagc ggcagattca acattgacag ttgcttctta cagcccagat
     3601 aaacagctca ctgaaactgc tacggtcaaa acagacgatc atcaagataa agaaatgcct
     3661 gttatttcaa caaaccgttt tgagccaaac aaggcttacg actatgctta tgctaatcgt
     3721 ggtacgaaag aggatgattt taaggatgtc gaaggtaaga ttgcccttat tgaacgtggc
     3781 gatattgatt tcaaagataa gattgcaaac gctaaaaaag ctggtgctgt aggggtcttg
     3841 atctatgaca atcaagacaa gggcttcccg attgaattgc caaatgttga ccagatgcct
     3901 gcggccttta tcagtcgaag agacggtctc ttattaaaag acaatccccc aaaaaccatt
     3961 accttcaatg cgacacctaa ggtattgcca acagcaagtg gcaccaaact aagccgcttc
     4021 tcaagctggg gtctgacagc tgacggcaat attaagccgg atattgcagc acccggccaa
     4081 gatattttgt catcagtggc taacaacaag tatgccaaac tttctggaac tagtatgtct
     4141 gcaccattgg tagcgggtat catgggactg ttgcaaaagc aatatgagac acagtatcct
     4201 gatatgacac catcagagcg tcttgattta gctaagaaag tattgatgag ctcagcaact
     4261 gccctatatg atgaagatga aaaagcttat ttttctcctc gccaacaggg agcaggagca
     4321 gtcgatgcta aaaaagcttc agcagcaacg atgtatgtaa cagataagga caatacctca
     4381 agcaaggttc acctgaacaa tgtttctgat aaatttgaag taacagtaac agttcacaac
     4441 aaatctgata aacctcaaga gttgtattac caagtaactg ttcaaacaga taaagtagat
     4501 ggaaaacact ttgccttggc tcctaaagca ttgtatgaga catcatggca aaaaatcaca
     4561 attccagcca atagcagcaa acaagtcacc gttccaatcg atgctagtcg atttagcaag
     4621 gacttgcttg cccaaatgaa aaatggctat ttcttagaag gttttgttcg tttcaaacaa
     4681 gatcctacaa aagaagagct tatgagcatt ccatatattg gtttccgagg tgattttggc
     4741 aatctgtcag ccttagaaaa accaatctat gatagcaaag acggtagcag ctactatcat
     4801 gaagcaaata gtgatgccaa agaccaatta gatggtgatg gattacagtt ttacgctctg
     4861 aaaaataact ttacagcact taccacagag tctaacccat ggacgattat taaagctgtc
     4921 aaagaagggg ttgaaaacat agaggatatc gaatcttcag agatcacaga aaccattttt
     4981 gcaggtactt ttgcaaaaca agacgatgat agccactact atatccaccg tcacgctaat
     5041 ggcaaaccat atgctgcgat ctctccaaat ggggacggta acagagatta tgtccaattc
     5101 caaggtactt tcttgcgtaa tgctaaaaac cttgtggctg aagtcttgga caaagaagga
     5161 aatgttgttt ggacaagtga ggtaaccgag caagttgtta aaaactacaa caatgacttg
     5221 gcaagcacac ttggttcaac ccgttttgaa aaaacgcgtt gggacggtaa agataaagac
     5281 ggcaaagttg ttgctaacgg aacctacacc tatcgtgttc gctacacgcc gattagctca
     5341 ggtgcaaaag aacaacacac tgattttgat gtgattgtag acaatacgac acctgaagtc
     5401 gcaacatcgg caacattctc aacagaagat agtcgtttga cacttgcatc taaaccaaaa
     5461 accagccaac cggtttaccg tgagcgtatt gcttacactt atatggatga ggatctgcca
     5521 acaacagagt atatttctcc aaatgaagat ggtaccttta ctcttcctga agaggctgaa
     5581 acaatggaag gcgctactgt tccattgaaa atgtcagact ttacttatgt tgttgaagat
     5641 atggctggta acatcactta tacaccagtg actaagctat tggagggcca ctctaataag
     5701 ccagaacaag acggttcaga tcaagcacca gacaaaaaac cagaagctaa accagaacaa
     5761 gacggttcag gtcaaacacc agataaaaaa aaagaaacta aaccagaaaa agatagttca
     5821 ggtcaaacac caggtaaaac tcctcaaaaa ggtcaatctt ctcgtactct agagaaacga
     5881 tcttctaagc gtgctttagc tacaaaagca tcaacaagag atcagttacc aacgactaat
     5941 gacaaggata caaatcgttt acatctcctt aagttagtta tgaccacttt cttcttggga
     6001 ttagcagctc atatatttaa aacaaaacgc caaaaagaaa ctaaaaaata gtattactta
     6061 aactgataag agcttattta atagagagaa ccaatttggt tctctctatt tt
//
LOCUS       DATRME010000099         6035 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRME010000099 DATRME010000000
VERSION     DATRME010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            131..1795
                     /locus_tag="VBC23_001912"
     CDS             131..1795
                     /locus_tag="VBC23_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6843832.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            1884..2807
                     /locus_tag="VBC23_001913"
     CDS             1884..2807
                     /locus_tag="VBC23_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6843833.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRSVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            2809..3726
                     /gene="srtC1"
                     /locus_tag="VBC23_001914"
     CDS             2809..3726
                     /gene="srtC1"
                     /locus_tag="VBC23_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6843834.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            3683..4534
                     /gene="srtC2"
                     /locus_tag="VBC23_001915"
     CDS             3683..4534
                     /gene="srtC2"
                     /locus_tag="VBC23_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6843835.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            4616..>6035
                     /locus_tag="VBC23_001916"
     CDS             4616..>6035
                     /locus_tag="VBC23_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6843836.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSN"
BASE COUNT         2140 a         1006 c         1230 g         1659 t
ORIGIN      
        1 agtaaaaata aacacgaatt taaaataagc aaatttttta agaaaatctg tgctaaactt
       61 taatagtttt gtgcttaata ataatcagca cttacaaaga acaaagggaa aagcgaggag
      121 agaactttta atgaaattat cgaagaagtt attgttttcg gctgctgttt taacaatggt
      181 ggcggggtca actgttgaac cagtagctca gtttgcgact ggaatgagta ttgtaagagc
      241 tgcagaagtg tcacaagaac gcccagcgaa aacaacagta aatatctata aattacaagc
      301 tgatagttat aaatcggaaa ttacttctaa tggtggtatc gagaataaag acggcgaagt
      361 aatatctaac tatgctaaac ttggtgacaa tgtaaaaggt ttgcaaggtg tacagtttaa
      421 acgttataaa gtcaagacgg atatttctgt tgatgaattg aaaaaattga caacagttga
      481 agcagcagat gcaaaagttg gaacgattct tgaagaaggt gtcagtctac ctcaaaaaac
      541 taatgctcaa ggtttggtcg tcgatgctct ggattcaaaa agtaatgtga gatacttgta
      601 tgtagaagat ttaaagaatt caccttcaaa cattaccaaa gcttatgctg taccgtttgt
      661 gttggaatta ccagttgcta actctacagg tacaggtttc ctttctgaaa ttaatattta
      721 ccctaaaaac gttgtaactg atgaaccaaa aacagataaa gatgttaaaa aattaggtca
      781 ggacgatgca ggttatacga ttggtgaaga attcaaatgg ttcttgaaat ctacaatccc
      841 tgccaattta ggtgactatg aaaaatttga aattactgat aaatttgcag atggcttgac
      901 ttataaatct gttggaaaaa tcaagattgg ttcgaaaaca ctgaatagag atgagcacta
      961 cactattgat gaaccaacag ttgataacca aaatacatta aaaattacgt ttaaaccaga
     1021 gaaatttaaa gaaattgctg agctacttaa aggaatgacc cttgttaaaa atcaagatgc
     1081 tcttgataaa gctactgcaa atacagatga tgcggcattt ttggaaattc cagttgcatc
     1141 aactattaat gaaaaagcag ttttaggaaa agcaattgaa aatacttttg aacttcaata
     1201 tgaccatact cctgataaag ctgacaatcc aaaaccatct aatcctccaa gaaaaccaga
     1261 agttcatact ggtgggaaac gatttgtaaa gaaagactca acagaaacac aaacactagg
     1321 tggtgctgag tttgatttgt tggcttctga tgggacagca gtaaaatgga cagatgctct
     1381 tattaaagcg aatactaata aaaactatat tgctggagaa gctgttactg ggcaaccaat
     1441 caaattgaaa tcacatacag acggtacgtt tgagattaaa ggtttggctt atgcagttga
     1501 tgcgaatgca gagggtacag cagtaactta caaattaaaa gaaacaaaag caccagaagg
     1561 ttatgtaatc cctgataaag aaatcgagtt tacagtatca caaacatctt ataatacaaa
     1621 accaactgac atcacggttg atagtgctga tgcaacacct gatacaatta aaaacaacaa
     1681 acgtccttca atccctaata ctggtggtat tggtacggct atctttgtcg ctatcggtgc
     1741 tgcggtgatg gcttttgctg ttaaggggat gaagcgtcgt acaaaagata actaaataaa
     1801 aggctacttc ttaagtaacc atgtttaaga aaagagaaat agccttattt ctctttttgt
     1861 cgtttttaaa ataaaggaac atcatgaaac aaacattaaa acttatgttt tcttttctgt
     1921 tgatgttagg gactatgttt ggaattagcc aaactgtttt agcgcaagaa actcatcagt
     1981 tgacgattgt tcatcttgaa gcaagggata ttgatcgtcc aaatccacag ttggagattg
     2041 cccctaaaga agggactcca attgaaggag tactctatca gttgtaccaa ttaaaatcaa
     2101 ctgaagatgg cgatttgttg gcacattgga attccctaac tatcacagaa ttgaaaaaac
     2161 aggcgcagca ggtttttgaa gccactacta atcaacaagg aaaggctaca tttaaccaac
     2221 taccagatgg aatttattat ggtctggcgg ttaaagccgg tgaaaaaaat cgtagtgtct
     2281 cagctttctt ggttgacttg tctgaggata aagtgattta tcctaaaatc atctggtcca
     2341 caggtgagtt ggacttgctt aaagttggtg tggatggtga taccaaaaaa ccactagcag
     2401 gcgttgtctt tgaactttat gaaaagaatg gtaggactcc tattcgtgtg aaaaatgggg
     2461 tgcattctca agatattgac gctgcaaaac atttagaaac agattcatca gggcatatca
     2521 gaatttccgg gctcatccat ggggactatg tcttaaaaga aatcgagaca cagtcaggat
     2581 atcagatcgg acaggcagag actgctgtga ctattgaaaa atcaaaaaca gtaacagtaa
     2641 cgattgaaaa taaaaaagtt ccgacaccta aagtgccatc tcgaggaggt cttattccca
     2701 aaacaggtga gcaacaggca atggcacttg taattattgg tggtatttta attgctttag
     2761 ccttacgatt actatcaaaa catcggaaac atcaaaataa ggattagcat gggacaaaaa
     2821 tcaaaaatat ctctagctac gaatattcgt atatggattt ttcgtttaat tttcttagcg
     2881 ggtttccttg ttttggcatt tcccatcgtt agtcaggtca tgtactttca agcctctcac
     2941 gccaatatta atgcttttaa agaagctgtt accaagattg accgggtgga gattaatcgg
     3001 cgtttagaac ttgcttatgc ttataacgcc agtatagcag gtgccaaaac taatggcgaa
     3061 tatccagcgc ttaaagaccc ctactctgct gaacaaaagc aggcaggggt cgttgagtac
     3121 gcccgcatgc ttgaagtcaa agaacaaata ggtcatgtga ttattccaag aattaatcag
     3181 gatatcccta tttacgctgg ctctgctgaa gaaaatcttc agaggggcgt tggacattta
     3241 gaggggacca gtcttccagt cggtggtgag tcaactcatg ccgttctaac tgcccatcga
     3301 gggctaccaa cggccaagct atttaccaat ttagacaagg taacagtagg tgaccgtttt
     3361 tacattgaac acatcggcgg aaagattgct tatcaggtag accaaatcaa agttatcgcc
     3421 cctgatcagt tagaggattt gtacgtgatt caaggagaag atcacgtcac cctattaact
     3481 tgcacacctt atatgataaa tagtcatcgc ctcctcgttc gaggcaagcg aattccttat
     3541 gtggaaaaaa cagtgcagaa agattcaaag accttcaggc aacaacaata cctaacctat
     3601 gctatgtggg tagtcgttgg acttatcttg ctgtcgcttc tcatttggtt taaaaagacg
     3661 aaacagaaaa agcggagaaa gaatgaaaaa gcggctagtc aaaatagtca caataattcg
     3721 aaataataaa attagaaccc tcatttttgt gatgggaagt ctgattctct tatttccgat
     3781 tgtgagccag gtaagttact accttgcttc gcatcaaaat attaatcaat ttaagcggga
     3841 agtcgctaag attgatacta atacggttga acgacgcatc gctttagcta atgcttacaa
     3901 tgagacgtta tcaaggaatc ccttgcttat agaccctttt accagtaagc aaaaagaagg
     3961 tttgagagag tatgctcgta tgcttgaagt tcatgagcaa ataggtcatg tggcaatccc
     4021 aagtattggg gttgatattc caatttatgc tggaacatcc gaaactgtgc ttcagaaagg
     4081 tagtgggcat ttggagggaa ccagtcttcc agtgggaggt ttgtcaaccc attcagtact
     4141 aactgcccac cgtggcttgc caacagctag gctatttacc gacttaaata aagttaaaaa
     4201 aggccagatt ttctatgtga cgaacatcaa ggaaacactt gcctacaaag tcgtgtctat
     4261 caaagttgtg gatccaacag ctttaagtga ggttaagatt gtcaatggta aggattatat
     4321 aaccttgctg acttgcacac cttacatgat caatagtcat cgtctcttgg taaaaggaga
     4381 gcgtattcct tatgattcta ccgaggcgga aaagcacaaa gaacaaaccg tacaagatta
     4441 tcgtttgtca ctagtgttga agatactact agtattatta attggactct tcatcgtgat
     4501 aatgatgaga agatggatgc aacatcgtca ataacgatgt tgtgaatggc ttacttactt
     4561 atcaaatagg tgactaatga tgattgtgaa taatggttat ctagaaggga gaaaaatgaa
     4621 aaagagacaa aaaatatgga gagggttatc agttacttta ctaatcctgt cccaaattcc
     4681 atttggtata ttggtacaag gtgaaaccca agataccaat caagcacttg gaaaagtaat
     4741 tgttaaaaaa acgggagaca atgctacacc attaggcaaa gcgacttttg tgttaaaaaa
     4801 tgacaatgat aagtcagaaa caagtcacga aacggtagag ggttctggag aagcaacctt
     4861 tgaaaacata aaacctggag actacacatt aagagaagaa acagcaccaa ttggttataa
     4921 aaaaactgat aaaacctgga aagttaaagt tgcagataac ggagcaacaa taatcgaggg
     4981 tatggatgca gataaagcag agaaacgaaa agaagttttg aatgcccaat atccaaaatc
     5041 agctatttat gaggatacaa aagaaaatta cccattagtt aatgtagagg gttccaaagt
     5101 tggtgaacaa tacaaagcat tgaatccaat aaatggaaaa gatggtcgaa gagagattgc
     5161 tgaaggttgg ttatcaaaaa aaattacagg ggtcaatgat ctcgataaga ataaatataa
     5221 aattgaatta actgttgagg gtaaaaccac tgttgaaacg aaagaactta atcaaccact
     5281 agatgtcgtt gtgctattag ataattcaaa tagtatgaat aatgaaagag ccaataattc
     5341 tcaaagagca ttaaaagctg gggaagcagt tgaaaagctg attgataaaa ttacatcaaa
     5401 taaagacaat agagtagctc ttgtgacata tgcctcaacc atttttgatg gtactgaagc
     5461 gaccgtatca aagggagttg ccgatcaaaa tggtaaagcg ctgaatgata gtgtatcatg
     5521 ggattatcat aaaactactt ttacagcaac tacacataat tacagttatt taaatttaac
     5581 aaatgatgct aacgaagtta atattctaaa gtcaagaatt ccaaaggaag cggagcatat
     5641 aaatggggat cgcacgctct atcaatttgg tgcgacattt actcaaaaag ctctaatgaa
     5701 agcaaatgaa attttagaga cacaaagttc taatgctaga aaaaaactta tttttcacgt
     5761 aactgatggt gtccctacga tgtcttatgc cataaatttt aatccttata tatcaacatc
     5821 ttaccaaaac cagtttaatt cttttttaaa taaaatacca gatagaagtg gtattctcca
     5881 agaggatttt ataatcaatg gtgatgatta tcaaatagta aaaggagatg gagagagttt
     5941 taaactgttt tcggatagaa aagttcctgt tactggagga acgacacaag cagcttatcg
     6001 agtaccgcaa aatcaactct ctgtaatgag taatg
//
LOCUS       DATRME010000100         5988 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRME010000100 DATRME010000000
VERSION     DATRME010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1695
                     /locus_tag="VBC23_001917"
     CDS             <1..1695
                     /locus_tag="VBC23_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6843837.1"
                     /translation="LFLVGCQNNKKVKTDKKVDITAYYYHNNTPQYSVQINAIHLQTA
                     LDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGIKNTRQDAIAIVRL
                     LLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVGLTAFENSAETQEY
                     VLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRIPIYSKQLTVRVSP
                     NFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLNSSGDETEQKELLQ
                     NSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKKGVIETKRSLISVD
                     NQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEYILGRLNADKKVSL
                     YQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDIDLNPRLWAYNYKKQ
                     VKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDSVSKAQFGNYNING
                     YIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFYITAIRFVFKRM"
     gene            1720..2544
                     /locus_tag="VBC23_001918"
     CDS             1720..2544
                     /locus_tag="VBC23_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6843838.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            2544..3266
                     /locus_tag="VBC23_001919"
     CDS             2544..3266
                     /locus_tag="VBC23_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6843839.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            3266..4324
                     /locus_tag="VBC23_001920"
     CDS             3266..4324
                     /locus_tag="VBC23_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6843840.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            4326..5261
                     /locus_tag="VBC23_001921"
     CDS             4326..5261
                     /locus_tag="VBC23_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6843841.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            5270..>5988
                     /locus_tag="VBC23_001922"
     CDS             5270..>5988
                     /locus_tag="VBC23_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6843842.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFV"
BASE COUNT         2117 a          839 c         1166 g         1866 t
ORIGIN      
        1 ctttttttag taggttgcca aaataataaa aaagtaaaaa cagataaaaa agtagatatt
       61 acagcttatt attatcataa taataccccg caatacagtg ttcagattaa tgctattcat
      121 ttacaaacag cattggataa agtgaggacg ggggagtttg agagcaaaga ctttacacac
      181 tatacttttg atagtatcaa gcggaagtac caagttagtg gtaaaaaagt ccttatggat
      241 aataactata gatttggttc tggtataaaa aatactagac aggatgctat tgctattgta
      301 aggttactgc ttgaaaagaa taaagattat ttaatttaca tgaatggctt agaagagcca
      361 agtgttttat ataatccaga aaataaacgt tataagttca caaataacaa aggaaaagca
      421 attggtaata ttccagttgg tttaactgct tttgaaaaca gcgcagaaac tcaagaatat
      481 gtgttgaaaa atattcagaa aaacgaaacg atttatcttg gtaatacccg agtagataat
      541 cctcgagtaa ctgttaataa caaaaaaaga gatactatag gagttgaata cggtaaacgt
      601 attacttata ggattccaat ttattcaaag cagctgactg tcagagttag ccctaatttt
      661 gtagtagata gtactaatta taattaccga ctttctcaag cgcctattat tgcggaagaa
      721 ggtaaagagg gacgtttgta tccaatagat agcagtatta gtgtcgatgg tgataaaatt
      781 gttttaaata gtagtggtga tgaaacagag caaaaagaat tattacaaaa tagcctgtat
      841 aaacttcact ctattgataa gttagacttt gatttgacaa atgctgatat taaagagtta
      901 gagattacag gacatgttgc ttcaaaaaga gattataatt tgtctgttgc tcaggaaaag
      961 aaaggcgtca tagagactaa acggagtcta atttctgttg ataatcaaaa acaagatcaa
     1021 gggatttttg tcgaggattc taaaggctat ataaagacac ctagtttgag cagttatggt
     1081 ataaattttg ccatgtatga cgacaatcaa aatagattat tgcaaggttc agagtatatt
     1141 ttaggaagat tgaatgcaga taaaaaagta tctctatatc aacctaatgg cttttggata
     1201 aatactcaaa aaacgattaa gagtttaaat aaagaatatc ttaaccaaaa tgcttaccat
     1261 attagaggaa ataacattat aggtattgat ggctattcaa atgacataga cttgaatcct
     1321 aggttatggg cttataacta taaaaaacaa gtgaaaagta accaatcatt atttaaatta
     1381 cgaggtttgt ctaataagta taagtatttc ttaatacaga caaaatctcc aaaaggctac
     1441 ttacaaagca ataaaccatt ttattttaca gtaaataaag atagtgtttc taaagcacaa
     1501 tttggtaact ataatataaa tggttatata atggacatgc agtacaacaa acaagaatac
     1561 aatgcacttg caaacactcc aaaaggtcaa aaaacaaaga aaactttcaa acccatgacc
     1621 ctagtgattc tttttgcagt agctttattt attttctata ttacagctat aagatttgta
     1681 tttaaaagaa tgtgagttaa ctgttagaca gaggtaatta tgaaagaaat gacggtctca
     1741 gaaataagag aagttcaact tgaaatgtta gcttatattg ataaagttgc gcgtgataat
     1801 aaaattgaat attctttagg cggaggcagc ctactgggag caatgagaca taaaggattc
     1861 attccatggg atgacgatat tgatttgatg ctagaaagaa gccagtatga gcgtttgatg
     1921 aaagcattag ctgatgctaa taatagcgat tttaaattgc tacatcattc tgttgaaaaa
     1981 aatctatggc catttgccaa gctgtatcac acaaagtcaa tgtacttaag taaaacagat
     2041 agaattcatc cttggacagg gatttttatt gatatcttcc cacttgaccg attaccagag
     2101 agtgcagaag aacgtcaaag attctttaaa aaagttcact ctgctgctgc aaatttgatg
     2161 tgtacgactt accccaattt tgctagcggt tcccgaaagc tttatgcaaa tgcaaggcta
     2221 attttggggc taccccgatt catcgcttat cacggccaag ctaaaaaacg tgctgaaatt
     2281 gttgatcaag tgatggaaac atataataat caagaggttc cttacatggg ttataccgat
     2341 tcaagatatc gcttgaaaga atattttcca agagaaattt tttctgagta tgaagatgtt
     2401 atgtttgaaa acattaaaac ccgaaaaatt aaaaatgagc atgcttatct gaatcaatta
     2461 tatgggggta gttatatgga attacctcca gaaagtaaga gagaaagtca tagttattac
     2521 acttggtact ggaaagaagg ttaatgatga atattggagt tatttttgca ggtggtgttg
     2581 gtcgtcgcat gaataccaaa ggtaaaccaa agcaattcct agaagtgcat ggtaaaccca
     2641 ttattgtaca cacaattgat attttccaaa atacagaagc tattgatgct gtagtagttg
     2701 tctgtgtatc agactggcta gattatatga ataatctggt agagcgtttc aaccttacga
     2761 aagtaaaagc agttgttgca ggtggagaaa ctggccaaat gtcaattttt aaggggcttg
     2821 aagccgctga acagctggct acagatgatg ccgttgtttt aattcatgac ggagtacgtc
     2881 cattgattaa tgaagaagtt attaatgcta atatacagtc agtaaaagaa acaggaagtg
     2941 cggtaacttc agttcgtgct aaagagacag ttgttctagt caatgattct tctaaaatat
     3001 ctgaagttgt tgatcgtact cgttccttca ttgctaaagc gccacaaagt ttttatctat
     3061 cagatatcct ttctgttgaa cgtgatgcca ttagcaaagg gattactgat gcaattgatt
     3121 ctagtacttt gatgggtatg tacaatcgtg aactcactat tgttgaggga ccatatgaaa
     3181 atatcaagat aacaacacca gatgattttt atatgtttaa agctttgtat gatgctagag
     3241 aaaatgaaca aatttatggg atgtaatgat gattaaattt agtaataaaa ttgtacaaga
     3301 agatttagca cttattagta accaaaatta tattaattgg gaaagaattg ctaataaagg
     3361 cgttttaata agtggttcta atagtatgtt agcgagttat atggtatttt tactagccta
     3421 tctcaatgag acaagaaatt accaaacgca aattattgcg acagctagaa acattgaaaa
     3481 agcccgtgat aaattttcag atttagttgg gaaagattat ttcaccttaa ttccttatga
     3541 tgtagaagaa cgcctagagt atgatggaaa agtggattac attatccatg cagcaagcaa
     3601 tgctagtcca actgctattt tgagtaatcc tgttagcatc ataaaagcta atacaattgg
     3661 tacattgaat ttattagact ttgcaaagga aaaaacaatt gaaaacttct tgttcttatc
     3721 aacgcgtgaa gtatatggaa catctattaa agaagtgatt gatgaagaag cttatggtgg
     3781 atttgatatt ttagctacta gagcttgtta cccagaaagc aaacgtatgg ctgaaactct
     3841 actgcaaagt tactatgatc aatataaggt accctttact attgctcgta ttgcacattc
     3901 atttggtcct ggtatggagc ttggtaacga tggtcgtatt atgaatgatc ttttatcgaa
     3961 tgtcattgat ggcaaagata tcgttttaaa aagtagtggt acagcagagc gtgctttctg
     4021 ttatttagca gatgctgtat cagggttatt tactatttta ttaaatggtg aagttggtca
     4081 agcttacaat gtagcaaatg aagatcaacc cattatgatt aaggatttgg cacaaaagtt
     4141 agttgatctc ttttctgata aaaatatttc agtagtattc gatattccta aaactatgag
     4201 tgcaggttat agcaaaatgg ggagaacacg tctcactatg gccaaattag aagcgttggg
     4261 atggaagcga gaagtttctc ttgaatctgg aatattaaaa acagtacaag catttgagga
     4321 gtaatatggt aaagacagca gttttaatgg cgacatacaa tggcgaaaaa tttatatctg
     4381 aacaacttga ttcaattcgc caacagacat taaaaccaga ttatgtatta ttgagggatg
     4441 attgttcaac ggatgaaaca gtcaatgtcg tcaataacta tatcgcaaaa catgagttag
     4501 aaggctggaa aattgttaaa aacgacaaaa acttaggctg gcgtttaaat tttcgtcaat
     4561 tacttattga tgtgttagcc tatgaggttg actatgtctt ttttagtgat caagatgata
     4621 tttggtatct tgataaaaac gaacgacagt ttgccattat gtcagataac cctcaaattg
     4681 aggttttgag tgcagacgtt gatatcaaaa cgatgtctac agaagccagt gttccacatt
     4741 ttctaacttt ttcttctagt gatagaatca gtcagtatcc taaagtatat gattatcaaa
     4801 cattccgtcc cggatggacc attgctatga agagagattt tgcgcaagct atcgctgata
     4861 attggacttt tggagctaat atttcgcacg ataatcttat cgagacaact gccagtatta
     4921 ttggaaatgg ttataacctt aacgaaagcg taggagttca tattcgatat ggaggaaacg
     4981 caagtggtaa tagcaaattt gttttgaatt ggaagtcgcc taaaacacaa catatcacag
     5041 gattaaaaac atttgttgat ttttacgaaa ccatgcagaa ggttgtcaac aaatatcaaa
     5101 atgatgtcgc tttaaatcaa cgcttagcct ttgaaaaaga aagattggaa gcttctaaat
     5161 tgtcattaat tcataatagt gcttttgtga ttaaaaattg gcgactctat aactctaata
     5221 gtggacgtat gagagattta ttatttgcgt ttaaaaagta aggaataata tggtaaaaac
     5281 agcagttttg atggcaacat ataatggtca aggtttcatc cacgatcagc tagattcaat
     5341 tagaaatcaa acgttgagac cagactatgt cttaatgagg gacgatggct ctactgatga
     5401 tactgtaaag gtggttgaag actatataaa agagcaccgt ttagatggtt ggtcaataac
     5461 tagtaatgat aaaaatttag gttggcgttt aaattttcgt caattactta ttgatgtgtt
     5521 agcctatgag gttgactatg tcttttttag tgatcaagat gacacatggt atcatcataa
     5581 aaataagatg caagttgata ttatggaaga acgccaagat ataaaccttc ttagcgccga
     5641 tattgatatc aaaaagttat cagaagaggc tactattccc aataatttta agttcaatgg
     5701 tgaggaaaca atttctaagt atccattaga cttttcatat cataactatc gtcaaggttg
     5761 gactttttgt ttacgcaagt cgtttgttga tcaagtaatg actttttaca gtgataattt
     5821 aattttgtca catgataatc tttttgcagg ggtatctggt gtcattggtt caggttacaa
     5881 tttaaacaga agtgttggtg aacataagcg acatgctgag aatgcaagtg ggaatatctt
     5941 gaatatgagc tcaagttacg aaaggcattt acaagaactg tattttgt
//
LOCUS       DATRME010000101         5806 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRME010000101 DATRME010000000
VERSION     DATRME010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            103..1248
                     /locus_tag="VBC23_001923"
     CDS             103..1248
                     /locus_tag="VBC23_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6843843.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1248..1883
                     /locus_tag="VBC23_001924"
     CDS             1248..1883
                     /locus_tag="VBC23_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843844.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1886..2812
                     /locus_tag="VBC23_001925"
     CDS             1886..2812
                     /locus_tag="VBC23_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6843845.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2813..3463
                     /locus_tag="VBC23_001926"
     CDS             2813..3463
                     /locus_tag="VBC23_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843846.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3525..4706)
                     /locus_tag="VBC23_001927"
     CDS             complement(3525..4706)
                     /locus_tag="VBC23_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843847.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4779..5632)
                     /locus_tag="VBC23_001928"
                     /pseudo
     CDS             complement(4779..5632)
                     /locus_tag="VBC23_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
BASE COUNT         1964 a         1033 c          932 g         1877 t
ORIGIN      
        1 ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacata ttgccaaagt
       61 tttgatatta ttactataat atagtttgta gaggagaata atatgggcca agaacctatc
      121 atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga agatattaac
      181 cttaaaattt accctggtga tttcgtttgt ttcatcggta cgagtggatc aggtaaaaca
      241 acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct attatttaag
      301 ggaaaagata tctctactat taaccccatt gaattaagac gcagaattgg atatgttatc
      361 caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct tgtaccaaaa
      421 ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat taaattagtt
      481 gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg tcagcaacaa
      541 cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat ggatgagcct
      601 tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa gtctcttcag
      661 gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc cctcaagtta
      721 gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac acccaatgat
      781 ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga gcgtctcctt
      841 catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc tgtttcaata
      901 actactgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg cgttgactca
      961 cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc tctaagcagt
     1021 aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt ttatgttatg
     1081 gaagacgact tacttagaaa tactgctgag cgtattttaa aacgtggttt aaaatacgct
     1141 ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc cctagttgat
     1201 atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg gtcaatttct
     1261 tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat atctcatttt
     1321 ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta acacgttttc
     1381 ccaaagtagc caaaattatc attgctatag ctagcatgct acaaactata ccaagtttag
     1441 ccttattagc tttaatgatt ccattgtttg gaattggaaa agtcccagct atcgttgctt
     1501 tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg aataatgtca
     1561 atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa tctatatttc
     1621 aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg tctactattt
     1681 atgttattgc ttgggctaca ttagcttctt atataggtgc aggtggactt ggtgatctca
     1741 tttttagcgg gttaaatctt tttcagccca aattaattct agggggcaca attcctgtta
     1801 ttatcctgtc acttattata gattatcttt taggattact ggaaacagct ctaacgccca
     1861 gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac agatatttac
     1921 actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta aaaaacctgg
     1981 tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatca tggcaaatat
     2041 tgtcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa gcaatcttgg
     2101 ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg ctgccacacg
     2161 ttatacagga acagacatca caggaactct tggcttaaaa gctgttaaag accctaaaga
     2221 agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt ggtatcctac
     2281 ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca gacagaataa
     2341 aatcaccaag atctctgatc tcaaaaagtt atcaacaact atgaaggcag gggttgatag
     2401 ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat acggttttga
     2461 attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg aaagtaacaa
     2521 aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg atttagaaat
     2581 tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg tcaacaattc
     2641 tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg gtaaaatcaa
     2701 tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag aaccttcagt
     2761 tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat aaatgaaaca
     2821 aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt atatcttaga
     2881 acaatttatc caccactttt taatttcaat ttacggcgtt attttagctg caattgttgg
     2941 tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg ttatgggaat
     3001 cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta tgttaggtct
     3061 agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc tgccaatcat
     3121 cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg ctgctaaagg
     3181 aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat ctatttctgt
     3241 tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag ccattggtgc
     3301 tttcgtcggt ggaggcggac ttggagatat catcataaga ggaactaatg caacaaatgg
     3361 tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt tctctgattt
     3421 aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa aaacttgtca
     3481 gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa ttgtttttta
     3541 ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac aataaagcta
     3601 acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg cataaacata
     3661 atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc gaccctgatt
     3721 tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa aagaaaacta
     3781 ccactgtaag atatccatat cggtaattca aatagagatg ggaatcccat aactatgact
     3841 cccaaactac tgaatactag catagatagc atagaattaa tactagattt aatttttcta
     3901 gcaataagcg ctccaataat tgatccgata gctgatatac taagtattgt tgcataagca
     3961 cctgaagttt tcaataggga gttagtaaaa ggtaaaagat aattgaacat tgctaggaaa
     4021 gtgtttaata aagcagcaat aattagaaaa aacataattt ctttttcttt tttgatgtaa
     4081 gtaaatccat ctgcaatatc agctaaaata ttttttatcg ctaccttttc ttgttttgaa
     4141 agctgtaccc tttccacttt aatagcataa agaaacagga aagagatcaa aaaagtaatc
     4201 gcatcaacaa ttaaagtaat cctaatacca aaattattaa aaataaggaa acctaaaaca
     4261 ggggaactta cactaataat ttgcacaatg gtttccagat tagcattata ggtaatgata
     4321 tcagctttat ctactatttc aggaatataa gatttataag ccggagaaga aaaagcattg
     4381 gaaaccgcta agatagcatt agcaactatt agaccataca ccattacttt atcatctcca
     4441 ataaaagaaa gtaagaaaca catgatagca cagatagcat ctgttattag taaaattttt
     4501 cttcgttgaa agcgatcagc taaggcgcct ccaaaagggt tgaggacaat agaaaccaac
     4561 aactctacta tctgataaat acctaaaatc ttttgtccta gaccacctat cgaagcaatc
     4621 caagtactat ttccataatc ataaagcaca tcaccaagtt tactgactgc tcgactagat
     4681 agtaatttta taccattaat attcataatt ccctccttag ttcgacatta tagcaaacca
     4741 aaaggagaaa tattaaaaaa tcccataaat gggatttttt ataatcttgt taccctatta
     4801 aagtgttcct gaaaattttc aacatgatgt ttagaaccaa ttgcttggaa gcaaaaaata
     4861 gcttgcttca tgctttgtat tcctcgacta tcccctttag cataggcaag aaatcctttt
     4921 tcatagagta aaataatacg gtgataagca ttccgctcat tatttaaaag tgcttcaact
     4981 ttctctagaa aaaacgttgc ttcttctaga gcgcgccgtt ctattagtgt ctctaccaca
     5041 tttaggaggg ttgcttcgac aacattgcgg tttgtataaa tatctcgata atattccttt
     5101 tgattgataa cctctttaac aagttggcaa atatgccttg tgttgtaata ccggtaaaga
     5161 tttccaatca aaatatagtc actaatttcc caactatcct tttgaaagag gtaatccgac
     5221 aaaaagcaaa ggtcttcttc cgacattttt cttgaggagt catattcaca tatcattcct
     5281 ctaaaaagaa ctatgtttaa acgacaacgt atatctgaac tatccttttt tagtttacct
     5341 tcttccacag aaatcatctt ttctaaccca gctacgtctc tttgataatg atattgagcc
     5401 atctgagaca ttactgatca gatttttgat atttatttac tctatccata aattcgctaa
     5461 ttgataaatc aattgcctct aaagccccta aaaattttgt taatgataaa tctgattcac
     5521 ccctctcaaa tcgtgataac tgagaaacag aaagttcatt tgaagcaacc tgtttaagag
     5581 aaaactgtcg attaagtcga aattccctaa aaatagggcc atatataaac attagtattc
     5641 ctttctaacc gtaatctcat tggtaaactt atgaggtaat ttcaataatt tatatttacc
     5701 aactttaacc aatatatgag ctgatatata gtcattttct tttatttgta atatcatatc
     5761 acaagttctt ctaaatcaac atatgttaat ttatctggga attttt
//
LOCUS       DATRME010000102         5653 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRME010000102 DATRME010000000
VERSION     DATRME010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            80..775
                     /locus_tag="VBC23_001929"
     CDS             80..775
                     /locus_tag="VBC23_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6843848.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            complement(933..1808)
                     /gene="tehB"
                     /locus_tag="VBC23_001930"
     CDS             complement(933..1808)
                     /gene="tehB"
                     /locus_tag="VBC23_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6843849.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            1951..3948
                     /gene="metG"
                     /locus_tag="VBC23_001931"
     CDS             1951..3948
                     /gene="metG"
                     /locus_tag="VBC23_001931"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6843850.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            4144..5481
                     /gene="brnQ"
                     /locus_tag="VBC23_001932"
     CDS             4144..5481
                     /gene="brnQ"
                     /locus_tag="VBC23_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6843851.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
BASE COUNT         1718 a          883 c         1108 g         1944 t
ORIGIN      
        1 taaaccaatc tattagaaaa aaagtgctga gcaagattgc ccagtcttat ttttgtttgc
       61 cataaagtta gaggaaaata tgtctaaaaa gtctcaccgt caatatcaaa tatatgaagg
      121 gctcaggtgt gcggttgcac tctgttttat ttcaggttat atcaatgctt ttacttatgt
      181 aacacaagga aaacgttttg caggtgtaca aacagggaat ttgttatctt ttgctattca
      241 tcttagtaat aagcattata gtcaagcgct tgcttttttg ctacctataa tggttttcat
      301 gttaggtcaa tcgtttactt atttcatgaa tcgttgggcg aataaacatc aactgcactg
      361 gtacctatta tctagttttg cgttgacaca ggttgctata gtaacaatta tattgactcc
      421 tttcttacca tcgtcgttta ctgtggcagg actagccttc tttgcttcaa tacaagtaga
      481 tacctttaaa tcgctcagag gagcacctta tgctaatatg atgatgactg gtaatatcaa
      541 gaatgctgct tatttattaa caaagggact ttacgaaaaa aattctgata ttttcttaat
      601 tgctagaaat acgattatta ttattggagg atttatcttt ggtgttgttt gctcgactta
      661 tttttcaagt aaactgggcg aatggagctt atcattaata ttaattcctc ttttatatgt
      721 caacttgctg ttaggccatg aattttataa cctacaagta gaaaggcact cctaatgtat
      781 ggcgttttat gttgaagagc catactaaag ctgattgtta caataatgat ttgaaatatc
      841 atctctatat attttatagg ataattatgt atgggtttag aatatactta ttacctaacc
      901 aaagataaaa gtgagtagat ttctactcac ttttatgatc ttaattttcg agctaataaa
      961 gtcgcaaact gtaattttaa gcgattaccg ttttcatcca ccctgtgcaa ttcacctaaa
     1021 ttttcattat atttgatgat ttcccagtca ttgtaataag atttcaattg tccttctttg
     1081 aaggtaaaag ggaatggaag cgggcaagga ttttcagcgg tatccatagc agaaacgata
     1141 agattgtatc ctccaatctg agtatgtgat tgcatttgta aaataatatc agaaatgcag
     1201 tcgggattta aaaacataaa taccacagtt gataaaataa aatcatagtg cccttcaata
     1261 gcagcagtat taatatcata cctttttata ttgtaaggaa gctcttcttc taatgccata
     1321 ttttctaaag ctacaaggct ctgtccgttt gaatcgacag aagtcacttg atgtcccagc
     1381 agcgataaat aaagtgagtt tcgcccttga ccacacccaa ggtctaatat attactcttc
     1441 tcagataaga gaggtgcact ggcgataacc tcagaatgtg tgcgcgtgag accatatttt
     1501 ttatggaagt aatcttcttt ttggcagtaa aaagttagat agcaacttaa gtctggacta
     1561 ttcggcgaaa ctttatgcca gacttgtgga tcaacaaaag gaatatcact actagcatca
     1621 aaaatatgcc gcgagatttc ctctccatca ggagataact ggtaaaatac taaagaacca
     1681 cttagaatgg ttagttttgc ccaggtgcct tccttagtat tatgcttttc taaaaatgcc
     1741 tttggaatag actgtgcagt ccaaaccggc atagttttgt aggggagaag tgtttttgta
     1801 ttaaccatat attaactctc tatttttaat acctttatta tagcaaaagt agatatatta
     1861 ttcagagata tatccagttg aaaaagattt tatgttataa tgtatcatga taaaaaacac
     1921 ctctcattag ggtggcttat aggagaaatc atgacagaaa aaaaatcatt ttacattact
     1981 accccaattt attacccatc aggtaaattg cacatcggtt ctgcctatac aacgatagca
     2041 tgtgatgttt tggcacgtta caaacgcatg atgggctttg atgttcagta tttgactggt
     2101 cttgatgagc atggtcagaa aattcaacaa aaggctgaag aagcaggcat tacaccacag
     2161 gaatatgttg atggcatggc tgaaagtgtc aaaacattat gggaactttt agatatttcc
     2221 tatgacaaat tcatccgtac aacagatact taccatgaag aagcggttgc taaaattttt
     2281 gaacaattgt tggcacaagg ggacatctat cttgatgagt ataccggatg gtattctgtt
     2341 tcagatgaag aattttttac tgaaagccaa ttagcagaag tttatcgtga cgaaaacggt
     2401 aatatgattg gtggtgttgc gccatctggt catgaagttg aaaaagtttc tgaagaatct
     2461 tatttcttcc gcatgagcaa atatgcagat cgtttaaaag cgtactacgc tgagcatcca
     2521 gaatttattc agccagatgg tcgtatgaat gaaatgctta aaaactttat agaaccaggt
     2581 ttagaagatt tagcagtgtc tcgtacaact tacacatggg gagttcaggt accatcaaat
     2641 ccaaaacatg ttatttatgt ctggattgat gctttgatga actatatttc tgctcttggt
     2701 tatggttggt ctgacgatct cagtcaatat cataaatttt ggccagcaga cattcatatg
     2761 attggtaaag atattcttcg tttccactct atttattggc ctatcatgtt gatggcgctg
     2821 gatttgccat taccaaaacg tctagttgct catggttggt ttgtgatgca agatggtaaa
     2881 atgtctaaat caaaagggaa tgtcgtttat ccagaaatgt tagtagagcg ttttggtctt
     2941 gatccactcc gttattatct catgcgttca ttgcctgttg gttcagacgg aacttttacg
     3001 ccagaagatt atgttggacg tattaactat gaacttgcta atgatttagg aaaccttctc
     3061 aaccgcacta ttgctatggt taacaagtat ttcgatggag aagtaccaag gtttgctgtt
     3121 gcaactgact ttgatgccga tttagcttct gttgcgacag atagtattga aaactatcat
     3181 aaacaaatgg aagcggtgga tttcccacgt gcccttgaag ctgtatggaa tcttatttca
     3241 cgaactaaca aatatattga tgaaacagca ccgtgggtac ttgcaaaaga cgaaacagat
     3301 cgtgataagt tagcagctgt aatgagtcat ttagttgcta gtctacgtgt tgttgctcac
     3361 ctcattcaac cgttcatgat ggaaacttcc gatgccatta tggaacaact tggtttaggg
     3421 gcaacattcg atcttgaaaa attgactttt gctgatctac cagaaggtgt tcgagttgtt
     3481 gcaaaaggtt caccaatttt cccaaggttg gatatggaag acgaaattac ttatatcaaa
     3541 gaacaaatga atgctggaaa agcaccagtg gaaaaggagt gggttcctga agaagttgaa
     3601 ttgacatcaa gcaaaggtca aatcaagttt gaggattttg acgctgttga aatccgcgtt
     3661 gcagaagtaa ttgaagttga gaaagtggaa ggttctgata aacttcttcg tttccgtcta
     3721 gatgctggtg atgagggcca tcgtcaaatc ttatcaggga ttgcaaaatt ttacccaaat
     3781 gaacaagagt tagtagggaa aaaactccaa attgttgcta acctcaaacc acgtaaaatg
     3841 atgaaaaaat atgtcagtca aggcatgatt ctctcagcag aacatgatgg taaattgaca
     3901 gtgttgacgg tcgattcagc agtggcaaat ggatcaatta ttggttaatc gaaaagagca
     3961 ttatgctctt tttttgtttt caagaaaaaa gtgactgaat agttggaaaa ttatgaaaat
     4021 gacctagaat ttatttttat ttttgatata ataaaagaca tcgattctaa gcatttagat
     4081 agaatttgat aaggagaatt tatggaaagg gtgtaaatag attatttaca ctaatggttg
     4141 cttatggtaa aaaaaggctt tttaacggga ttactcttat ttggtatctt ctttggtgca
     4201 gggaacctca tttttccacc agcattaggt gtagcatctg gacaagattt ctggccagcg
     4261 attttaggat tttgtttgtc aggagttgga ttagctatta ttaccctcct actaggcaca
     4321 ctaactaatg gtggttataa gacagagatg tcggaaaaat ttagtccgtg gttttcttta
     4381 acatttttag ttatactcta cctaactatt ggtccacttt ttgctattcc tagaacagca
     4441 acggtttctt ttgaaattgg ggttgctcca attgtagggc atagtccgat tgctcttttg
     4501 tgttttacag cctgtttttt tgctgcagct tattatctcg ctattagacc aaatggtata
     4561 ttagatagtg ttggaaaaat cttaacgcct gtttttgcct ttttaattct ctctttggtt
     4621 gttgttggag ctattgctta tggaaacttg gagagtgcta aggcgagtgc tgactatgca
     4681 ggaaaagcgt ttggaagtgg tgtcttagca ggctataata cacttgatgc acttgcagca
     4741 gttgcttttt gtctagtagc aacagaaacg cttaaaaagt ttggttttaa aactaaaaaa
     4801 gaatatttat caaccatttg gattgttgga attgttacta gtttagcatt tagtatttta
     4861 tatattggct taggtttttt agggaataag tttcctgtcc cagctgatat tttagctgac
     4921 ccaaatgtta ataaaggagc ttatgtatta tcacaagcat cttataaatt atttggaaat
     4981 tttggtcgtt atttcttgag tattatggta acattgacat gctttacaac aacagttggg
     5041 cttattgtgt cagtatctga attttttgat aagaactttc gttttgggaa ttataaatta
     5101 tttgctactg tttttacttt gattggcttt ttaattgcta atcttggttt aaatgctgta
     5161 attacttttt cagttcctgt gctaacttta ttgtatccga ttgttattgt tattgtctta
     5221 attattttga ttaataagtg gttgccgctt tcaaaaaagg gaatgtcttt gacaattggt
     5281 cttgtcactt tagtgtcgtt tgttgaggtt ctagcgggac aatggcaaga aaaaacatta
     5341 actcagttag ttggattcct cccctttcat actatttcaa tggggtggct agttccaatg
     5401 ttgattggta tagtattttc tttggtgctg tcagataaac aaaaaggcca agcatttgat
     5461 ttagagaaat ttgaaggata gcactatttc aattttatag atatgtcaaa aaggttaaaa
     5521 cttagtttta gcctttttta tatcagattt ttttaaatga tatatagatt gcagtaaggt
     5581 gtgattaaac taagatataa acacttttta aaaattattt tataacagct gtggattgaa
     5641 aatatacatt ttt
//
LOCUS       DATRME010000103         5633 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRME010000103 DATRME010000000
VERSION     DATRME010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(141..314)
                     /gene="secE"
                     /locus_tag="VBC23_001933"
     CDS             complement(141..314)
                     /gene="secE"
                     /locus_tag="VBC23_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6843852.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(350..502)
                     /gene="rpmG"
                     /locus_tag="VBC23_001934"
     CDS             complement(350..502)
                     /gene="rpmG"
                     /locus_tag="VBC23_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6843853.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(551..2872)
                     /locus_tag="VBC23_001935"
     CDS             complement(551..2872)
                     /locus_tag="VBC23_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6843854.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            2916..3800
                     /locus_tag="VBC23_001936"
     CDS             2916..3800
                     /locus_tag="VBC23_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6843855.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            complement(3825..5465)
                     /locus_tag="VBC23_001937"
     CDS             complement(3825..5465)
                     /locus_tag="VBC23_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843856.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVFIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
BASE COUNT         1769 a         1086 c          855 g         1923 t
ORIGIN      
        1 tatttgctta ataatctagt aaaaacaatc aaaagattat ttttttataa tttaatcgga
       61 tatatatgtt attttccaca caaaaaaacc cttagtaaaa tctaagggtt tgggttgttt
      121 ctatctaaaa taatctatta ttaaaatcga ttaatcaagt ccatgactcc tgcagctaac
      181 agtttgtcaa agatgtagat aacgattgtg aaaaatacag tgtattccaa gatggaaata
      241 aaatctttcc aacgttgttt tcgatttggc caagtagtgt cacgcaatac ttgaaaaatc
      301 cctttgacaa acattatcag ttctcctttt tgcagtttgt tacgctatat caacgcgttt
      361 ctttgtgtat tgtatatttc ttacaatatt tacaaaattt atttacttct agtcgtgttg
      421 gcttgggggt gctacttact ccgattgagt aatttcggct tccgcattct gtacacgcta
      481 ggcttgcttt cttttgtgcc ataacgcctc cataagtgtt attttaacat gatttgtata
      541 atcctgcaag ctatctaaag tagtccttta gactattcca agcatctttt accttctctt
      601 ggaaatttga atcttccaca gctctagaaa tatcttgtgc agccttactt gccgaatctt
      661 ttaaatcatt aataattgag cgtgattctt ggttcgttgt attactagca tcttgtgcat
      721 tataaactga accaataccg ttttggaaat aagcattctc aatatgagta aaactagacc
      781 ccttagtata cggtagtata tatgaagctt gcgtgctaaa tatatttgaa gctgtacctg
      841 cactagaatc tgtcaaataa tggtgtttat ctgtgttctt aaagccaacc cattgactaa
      901 taacgacatc aggcgtatag ccgactaccc actggtctcc agataaattg gggttaaaat
      961 ctgtttcggt tgttccagtt ttacctgcca ttgtataacc ataaacattg gcattaatag
     1021 ctgtaccgtt tgagaatgtt cctaacatca tactagtcat tttactagcc actgagcgac
     1081 taataactcg cttaggctta tctgtaaatt gctttactaa ttttccatta gctgtctcaa
     1141 ttcgagtaat caaatgagcg cgatgcatta taccatcatt tgcaaaagtt gagtaagctt
     1201 gtgccatttc taaaggatta gtagttacac ttccacctag cgccactcct agctctttgt
     1261 ttgctgatga catatttaga ccaaacttcc tgccataagt aaaagcttta ttgattccta
     1321 gtttatctaa agtataaaca gcaggaatat tataagaatt agctagtgct tgatacatcg
     1381 ggatactttc agtctcaatt ccaccatagt tacttggttt ataaccatga aaatcttgaa
     1441 ctttattagg aagttcttta tctattgacc atcctgaagc aatagctggt gaataaacaa
     1501 ctaaaggttt tatggttgag gctggacttc tctttgattg agtcgcgtag ttaaaacttc
     1561 taaactgtgc atctttagta ctttgaacac gtccaactaa acctcttaca ccaccagttt
     1621 ttggatctag agctacgcta gcaccttggg cagattgacc atcatagtca gatacaggga
     1681 agaggctagt atcatcaaat gttttttgca ttcctgattg ataattttga tctaatgcag
     1741 tgtaaatctt atagccatta ttgacaatat ctttttctga aataccataa gtatcaatgg
     1801 cttcattgac aacagcatca aaataggatg ggtacttgta atcattaatt ttaccagcat
     1861 aggtatctgc taatctattt ttcataccaa ttgaagcagc ttctttagct tgagacttgg
     1921 ttagttgacc tgcatcaacc atcgctgcca aaacagtatc tcttcggtta gttgcatttt
     1981 caacagaata ataaggatta taaacttctg gtcctttaag cattccagca agtgtagcag
     2041 cctcatctac tgtcaaatta gcagcactgg ttccaaaata tttcctacta gcatcttcca
     2101 caccccaaac accattacca aaataagagt tattaagata catggtcaga atctcagcct
     2161 tgctatattt cttagtcaat tctagcgcta aaaagaattc acgcgccttg cgtttaatcg
     2221 tttggtcctg tgacaagtaa gcattttttg ctaattgctg ggtaatagtt gagcctccgc
     2281 caaatttccc aagtgtagct actgccaaaa aaaatcgctt aaagtttaca ccattatttt
     2341 cataaaaagt tctatcttca gttgctataa cagcattttt gagatgatca gaaatagcat
     2401 ttaactctac ataagttccc ttttgtcccg aaagttttcc agcgtattct ttattcttat
     2461 cataaatggt ggtggttgtt tttagagcac tctgtaaatc actaactgta gcagttttag
     2521 aaagataaaa aagatagcta ccaaatataa gaataaaagc cattaaggcg ataaaaagca
     2581 acttcccaat acgatagcgc cgccaaaaac gacggatagg attcttagga gaagggaggt
     2641 aacgatcaac tttttgtagc cattcaagtc gagatttagc attttccttt tggtgacgaa
     2701 tgcctcgctg atactgggat ttactaggtc gggtaatatc atcaatatca tagacttctt
     2761 cgccatccaa attgctagtt tcttctctag tttcttgttc attttttctt gtcatttcgt
     2821 cgttgtcttc atcaacacga aataagtcta taaacttatc aaataatttc atagacttat
     2881 tatatcaatt ttcaataaaa tgctataata aaaccatgtc attttcatta aaaattagaa
     2941 atccatacgg tgaacatacc gttaaagaac tccttgaaga ttattttttg attccacgta
     3001 agattagaca ttttttgcgt gttaaaaaac atgtacttat aaacaatgaa ttcattaatt
     3061 ggcaaactgt cgtccaagaa aacgatacta ttaccttaat ctttgatgat gaggattacc
     3121 ctactaaaaa aattcctctg ggcagagcag agcttattga ttgtctttat gaggatgaac
     3181 atcttattat cgttaataaa cctgaaggta tgaaaactca cggtaaccaa ccaaatgaaa
     3241 tagcactgtt aaatcatgta tctgcctatt ctggacaaac atgctatgtt gttcatcgcc
     3301 tagatatgga gaccagtgga gctgttttat ttgctaaaaa tccatttata cttcccctta
     3361 tcaatcaacg cttagaacga aaagaaattt ggcgtgaata ttgggcttta gttgaaggaa
     3421 aattttcacc taagcatcaa gttttgagag acaaaattgg acggaaccgt catgacagac
     3481 gtaaacgaat cattgattct aaaaacggtc aacatgctat gacaatcatt gacgttttga
     3541 agtatatcca aaatagtagt ctcataaaat gccgactgga aaccggaaga acccatcaaa
     3601 ttcgcattca cttatctcat cacggacatc ctttaatagg agatcccctc tacaaccctt
     3661 cttctaataa tgaaaggtta atgctacacg ctcaccgatt gactctatcc catccattaa
     3721 cttgcgaaac tattagcgta gaggcccctt catctacttt cgagaaggtt ttaaacaatt
     3781 ataaaaaagg agttggataa taattatcca actcctttta atcattattg cttagttaat
     3841 ggcttactga gtagtggttt atggtaatca aattctccat cactagatgt ctgccagatt
     3901 actgggatga tcttattccc ttcaattttt attccatagg cgtatttaac tttaagacca
     3961 ccgatacctc ctgttacaaa agttgtagta tcagtacctg attcatattg ataaaggtag
     4021 agcccatttc ccttatcctc caacttcgta actttagcag aatggttact ttccttagat
     4081 ttactatcct tgtaaacttt tttctgagta actttaccat ccttatcaaa agtcatcgtt
     4141 actgtaccgt cctcaacttc acctttccaa cttcctacaa ggttctctgg aaaaataaaa
     4201 acgggatcag ccttaacttt attagtttcc ttagctactg tgatatcttt tttaccatca
     4261 ttagaaataa ggtaactatt tcccgatttt ttcataataa aattaccagt taaattctga
     4321 ataatgtggc catatgtttt cttatcagga tctgttgatt tatcataatt aaaatcataa
     4381 tcggcagcaa aattaacttg ataactttct ttcccaacct gtgtcatttt attaaggaca
     4441 atattaggaa tagtgaaatg gctcgcttta cgattatcag ttacaaattt tgctttaata
     4501 ctttctttca atcctttgta aaaatcattt tcggcacctt tttcaaaaac tgttcctaaa
     4561 gtctgagggt cttgcccagt tgatacataa agtatcaatt gattgaaagc agttatcaat
     4621 ttctgtccag cttctttttc atttaataaa ccatcaacat ctaatttgat tgtctgatta
     4681 tcagcgatag agattaattt ttgtttatga gaggttatct caccatcatt aaaaactttt
     4741 ttgatataag cttttgaacc atcagtcact ggataattct ctacggaata gctaccatct
     4801 tttaatttag caatacgatt atcaccaaaa taaagattac catccataag attacttgtt
     4861 actgtaaaag atttaaaagc gaccgataaa tcaatagttt ggtttttacc atcataatct
     4921 tttttgagtt taatctcttt tccgtcacta gtcccttcaa ggctagcagt ataatgggtt
     4981 ctaggtaaac gttccaccgt tactgaaaaa tgatctgaat tgcttgtcgc gacctttttt
     5041 tgattcaaca aaacatccaa tttaggaaca ttggttttca aggtcaaaga cataggctta
     5101 ttagcaatac gataatcagg gaatatacct aagcgactac ctacacgctt tagatacatt
     5161 gaatcatttt tgtttccttc ttttatttcc tgccctaatt tacgcgcctc acttttagac
     5221 aaaggctcaa aattagcgta attggcctca tcatttgtga tagattctcc cgtatctgac
     5281 caaaccatgt attctttaat attatcaaac gcttgacctg acgtacgaga acgtgctgct
     5341 acaaaacgac taagtacttg tccctttgaa aaataaaaga atcctagact cagaagtgat
     5401 actaaaacta taccaaaaat aactaagcac actttaacaa cttttttctt aaaaaaatca
     5461 aacatacctt atcctctcat tcactttata ttaaagtaaa ttgcttaaag aaaagttaca
     5521 acactgataa aacagctgta tttgtacttg taaccgctat ttttctatta taacaaaacg
     5581 aaaaaaggat tgaaaccata atccgatttc aacctctaaa atttttgtct atg
//
LOCUS       DATRME010000104         5420 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRME010000104 DATRME010000000
VERSION     DATRME010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(53..1387)
                     /gene="trmFO"
                     /locus_tag="VBC23_001938"
     CDS             complement(53..1387)
                     /gene="trmFO"
                     /locus_tag="VBC23_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6843857.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(1501..2199)
                     /locus_tag="VBC23_001939"
     CDS             complement(1501..2199)
                     /locus_tag="VBC23_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6843858.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            2412..3974
                     /gene="guaA"
                     /locus_tag="VBC23_001940"
     CDS             2412..3974
                     /gene="guaA"
                     /locus_tag="VBC23_001940"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6843859.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4244..5371
                     /locus_tag="VBC23_001941"
     CDS             4244..5371
                     /locus_tag="VBC23_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6843860.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFKD"
BASE COUNT         1610 a         1004 c          943 g         1863 t
ORIGIN      
        1 aaatcaatat agaaaatttg accttgcttg ttggcaaagt caaatgaata tatcaatagt
       61 tgagaaattt ttctaaatct ttcaaagctc gtgtagcaat agcttcataa cgctctttct
      121 tatcacgaat tcgtggacct tctaattctt tgatgatacc aaagttaaca ttcattggtt
      181 ggaaatgttt gctgtctgta tgtgtaatat aatgtggcag tgcaccaata gcagtagttt
      241 gtgggaaaac tacttcactc tccccattga agcgacggac agcattgatt cccgctacaa
      301 gaccacttgc tgccgattct acataacctt caacaccggt catttgtcca gcaaagaaaa
      361 gatttgggtt cttacgtgta gcaaaggttt ggtttaaaag atttggtgaa tccatataag
      421 aattgcgatg cataacacca taccggacaa attcagcatt ttcaagacca ggaatcattt
      481 gaaatacacg tttttgttct ccccacttaa gatgagtttg gaaaccaact atgttataaa
      541 gagagcctgc agcattatct tgacgtaatt gaacaacagc atatggtgtt ttaaattcac
      601 catctcgcgg tcctttgtag tcctcaggat actccaaacc aactggtttc attggaccat
      661 aaagcatcgt tttaatacca cgcttagcca tcacttcgat aggcatacaa ccttcgaagt
      721 atttttcttt ctcaaaagaa tttagtggtg cttcctcagc tgtagttaaa gcttcatgaa
      781 aagccataaa ttcttctttg gtcattggac aatttagata agctgcttca cctttgtcat
      841 acctagactt aaggtaaact ttattcatgt caatcgtatt tttatcgaca attggagcgg
      901 cggcatcgta aaaataaaaa ccatcaccac cattaagttc atggatttta gctgccagac
      961 tatcacttgt taatggtcct gtagcgataa ctgtaatagc atctccaggg atatctgtga
     1021 tttcatcacg aattacttcg attaatggat gtttatggat ttcctcagta acagcttcag
     1081 aataaccttc tctatcaacg gccatggctc cacctgctgg aactcgatgt gcctccccat
     1141 ttctcataat gatagaatcc aaacgacgca tttcttcttt taaaagacca actgcatttg
     1201 ttagagaatc accgcggaat gaattggaac atactaattc tgcaaaatta tctgttttat
     1261 gttgaggggt tgatttaacc ccacgcattt cataaagctt aactggtatt ccacgtttag
     1321 caatttgata agctgcttca gaaccagcca aaccagcgcc gataacatta atgtaagatt
     1381 gagacatgaa ctaatacctc ttgaaagtag tcaactactt cctcaaatct ttctatataa
     1441 tttatttttc cattataaca tacatagcta cttcttcaaa aaacgttatt cattaaacta
     1501 ctagttattc tctaaataaa actcaaaacg atctccaaca tattgcccat gaacatattc
     1561 aaaaggctta ccatcagtaa agtatgaaac ttgtgtcaat gccaaaatag catggcctgc
     1621 gttaacttct aaatgcgaag caacacgttc actagcattt ctagcatata tagtttgttg
     1681 gcttttccca atttcgtagc cattctctgt taaggtttta aagaaatgtt ctgtaacctc
     1741 tgattgttca aaacctttaa ttaaattctc tggtatagag gctacctcat aaaccaacgg
     1801 taccttatca gcataacgta ccctttccat acgaaccaca taatctgact gtgataaatt
     1861 taatttttga atttcggttt cattagctag ttttctttga aatgagatta gtttactgga
     1921 tggcttacgc ccttgagaat tgacaatttc tgtaaaacta gttgttcccc gcattttttc
     1981 ttgaacacga tgactagcaa cataggtgcc actaccaact cgtctctcca aaataccttc
     2041 ttcaactaac aatgtaattg attggcgaag tgtcatacga cttacactat aatcatccgc
     2101 taaatctctt tcgctaggta accgctgccc aatcttccaa gtccctttat cgatttcctt
     2161 tttaatagcg tcgtgaattt taatatatgc tggtaacaat attctctcct cctagaatct
     2221 acacttcatt ttaccatact tctatatatt tctaactcta aaatcgtgaa ataattcaga
     2281 aaaatcataa gcaatatttc ttgataagaa agcgtttaat caattatttt aatgatgtta
     2341 atttatataa aatatgctaa aatggtatag gtacagctgt ttttaaaatt agctacaaag
     2401 aaagggaaac aatgactgat atttctatat tgaatgatat ccaaaaaatt atcgttctag
     2461 attacggtag tcaatataac cagcttatcg cacgtcgtat ccgtgaattt ggtgtatttt
     2521 ctgagttaaa atcacataaa attacggctg atgaaattcg cgatattaat cctattggta
     2581 tcgtactttc aggtggtcca aattccgtat acgctgatgg cgcttttgga atcgatgaag
     2641 aaatttttga attaggaatt ccaatcttag gaatttgtta tggtatgcaa ctgattaccc
     2701 ataaactcgg tggtaaggtt cttcctgcgg gagaagctgg tcaccgtgag tatggtcaat
     2761 cagcacttcg cctacgctca gaatcagcac tctttgctgg cactcctcaa gaacaacttg
     2821 ttttgatgag ccatggtgat gctgttactg aaatccctga gggtttccat ctcgttggtg
     2881 actctgttga ctgtccattt gctgcaatgg aaaatactga aaaacaattt tatggtatcc
     2941 aattccaccc cgaagttcgt cactcagtat atggcaatga tatccttaaa aattttgctg
     3001 ttaatatctg tggtgcacgt ggtgattggt caatggataa cttcattgat atggaaattg
     3061 caaaaatacg tgaaactgta ggcgatcgta aagtacttct tggactatct ggcggtgttg
     3121 attcatctgt tgtcggagta ctcctacaac gtgctatcgg tgatcaatta acatgtattt
     3181 ttgtagatca tggtttatta cgtaaaaatg aaggtgacca agttatggat atgcttggtg
     3241 gtaaatttgg acttaatatt atccgtgttg atgcttcaaa acgcttccta gatcttcttt
     3301 ctggtgttga agatcccgag agaaaacgaa aaatcatcgg aaatgagttc gtgtacgttt
     3361 ttgatgatga ggcaagtaaa cttaaaggcg ttgatttcct tgctcaaggt accctctaca
     3421 cagatattat tgaatctggg acagaaacag cccaaacaat caagtcacac cataatgttg
     3481 gtggattgcc agaagatatg caatttgaat tgattgagcc acttaacaca ttattcaaag
     3541 atgaggttcg cgctcttggt actgcacttg gaatgcctga tgaagttgta tggcgtcaac
     3601 cattccctgg gcctggtctt gctattcgcg ttatgggtga aattaccgaa gaaaaacttg
     3661 aaacagtccg cgaatcagat gctattcttc gtgaagaaat cgctaaagct ggtctagatc
     3721 gtgacgtatg gcagtatttc acagttaata ctggcgtacg ttctgtcggt gttatgggag
     3781 atggtcgtac ttatgactat acaattgcta tccgtgcaat tacttcaatt gatggcatga
     3841 cagctgactt tgcgcaactt ccttgggatg tacttaagaa aatttcgaca cgtattgtta
     3901 acgaagttga tcacgttaac cgtattgttt atgatattac aagtaaacca cctgcaactg
     3961 ttgagtggga ataaaaactt agctacttta agtagctaag tttttttact cttatttctt
     4021 gaataaggga tacttacact taaaatatca tttttatgta gttcgatagt tacaagttca
     4081 ttattcttaa attgaatatt tttcagtata tttttccatt ttcttgataa taatatgaaa
     4141 aagacagaaa agaaatattc ctagatttta ggcttaaaaa atggtattct aaatattcag
     4201 tacaatatga ttgatatcat tttattaata ttaggaggaa aaaatgcata ttatcattca
     4261 aattacaatc attttgttgg caagcgttct tgcaactctt atttcaaaaa gaattggaat
     4321 accggctgtt gttggacaac tacttgtagg tatcatcatc ggacctgcaa tgctaggact
     4381 tgttcatcaa aatcaggtat tacatgtcct gtcagaaatt ggtgttatct tactcatgtt
     4441 tttagcgggt cttgaagcta attttgatct tttaaagaag tatcttaagc ccagtctact
     4501 ggtcgcaatt actggtgtta ttgttcctat ggctctcttc tacttcttga cacgcttgtt
     4561 tgggttccaa atcaatactg ctatctttta cgggctcgta tttgctgcaa ctagtatttc
     4621 tatcacagtt gaagttttac aagaatacaa tcgtgtaaaa acagatactg gtgctattat
     4681 tttaggtgct gctgttgctg atgatgtctt agctgttttg cttctgagtg tctttattgc
     4741 aacaaatgga agctcttcta atatcggatt acaaattatc attcaattgt tattctttgt
     4801 tttcttattt atttgtatga aataccttgt acctgctctt ttcaaattaa tcgaaaaggt
     4861 tcatttcttt gagaaatata ctattcttgc tatccttatc tgtttttctc tatcaatttt
     4921 agccgataaa gttggaatga gttctatcat tggttcgttt tttgcaggat tagctattgg
     4981 acaaacttct tttgttgata aggtagaaca taaaatttca cttcttagtt acaccttctt
     5041 tatcccaatt ttcttcgctt caattgcact acctcttaaa ttcgatggaa tgatgtctca
     5101 ccttcacacg atattaatct ttactgcttt ggctgtttta tcaaaattaa ttccagggta
     5161 ctttgtcggt cgaggcttta atttttcaaa attagaatct ttaacaattg gtggtggaat
     5221 ggtcagtcgt ggcgagatgg ctctcattat agttcaagtt ggattagctg ctaaaattat
     5281 ttcaagcact acttattccg agcttgttat tgttgttata ctgtcaacta ttattgcgcc
     5341 atttattctt aaatattcat ttaaagatta gtaaaaaaac acaaacattt atttagggta
     5401 aaaaaagtct agcttataag
//
LOCUS       DATRME010000105         5178 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRME010000105 DATRME010000000
VERSION     DATRME010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..869
                     /locus_tag="VBC23_001942"
     CDS             <1..869
                     /locus_tag="VBC23_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6843861.1"
                     /translation="KKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFHEV
                     FGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNPLL
                     AALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVGFL
                     AFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGVTE
                     IPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQKN
                     HGRLDEDILMPM"
     gene            935..2554
                     /locus_tag="VBC23_001943"
     CDS             935..2554
                     /locus_tag="VBC23_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6843862.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            2677..>5178
                     /locus_tag="VBC23_001944"
     CDS             2677..>5178
                     /locus_tag="VBC23_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6843863.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DSFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKVGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILI
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQDKQTILYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVRKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKGIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKF"
BASE COUNT         1674 a          853 c          851 g         1800 t
ORIGIN      
        1 ctaaaaaaat taagaatttt atactattaa atattggatc tctaattgtt gcatttggtt
       61 tcaacgcttt ctttcttcat aatcatattg tcacgggggg gattagtggt ttggcagtta
      121 gttttcatga agtatttggt tgggattctg ctttttttat tatgattatc aatattccat
      181 tgctccttct ttgctacttt ggcttaggta aacaaacctt tttaaaaact gtctatggtt
      241 cttggatttt tcctgttttt attaagttaa cacaaagtgt accaactttg acccacaacc
      301 cactcctcgc agcacttttt ggaggtgtta ttgtaggatg tggtttgggg attgtttttt
      361 ggagcgactc ttcaactggt ggaacgggga ttatcattca attcttagga aaatatactc
      421 ctataagcct tggacaaggg gttatattga ttgatggact tgttacaatt gttggtttcc
      481 tagcttttga cagtgatacg gttatgtttt ctattattgg gttgataact attagttata
      541 ttattaatgc tatccaaact ggatttacaa ccttaagcac tgtcttaatc gtttctcaag
      601 agcaccaaaa aattaagaca tatatcaata ctgtcgcaga tagaggagta acagaaattc
      661 ccgttaaagg gggatattct ggaactaatc aaatcatgct tatgacaact attgctggtt
      721 atgagtttgc taaattacaa gaggcaatag cagaaattga cgaaacagcc ttcataacag
      781 taactccaac atcacaagct tctggacgtg gatttagtct tcaaaaaaat catggacgtc
      841 ttgatgaaga cattcttatg ccaatgtaat cgattgataa ttaaagcttt ttctagtaga
      901 atagcagata gaatatacac aagaggtaac aaaattgctt acagtttcag atgtttcact
      961 acgttttagt gatcgcaaat tatttgatga agttaacatt aactttacag caggaaatac
     1021 ctatggtctt atcggagcaa atggcgctgg taaatcaact ttccttaaaa ttttagctgg
     1081 tgatattgaa ccaacaacgg gacatattgc acttggccca gatgaacgcc tttctgtact
     1141 tcgccaaaat cattttgatt atgaagatga gcgcgtcatt gatgtcgtta tcatgggtaa
     1201 tgaaacactt tatagtatca tgaaagaaaa ggatgctatc tacatgaaag aagatttttc
     1261 tgatgaagat ggtgttcgcg ctgcggaatt agaaggagaa tttgctgaac tcggtggatg
     1321 ggaggctgaa agtgaggctt ctcaattact ccaaaatcta aatatttcag aagagcttca
     1381 ttatcaaaat atgagtgaat tagctaatgg agacaaagtt aaagttctcc ttgcaaaagc
     1441 tctctttggt aaaccagatg tacttctcct tgacgagcct accaatggac ttgatatcca
     1501 gtctattact tggttagaag atttcttgat tgactttgaa aatacagtta ttgtagtttc
     1561 ccacgaccgt catttcttga ataaagtatg tacacatatg gctgaccttg attttggaaa
     1621 aattaaactg ttcgtcggca actatgattt ctggaaagaa tcatcagaat tagcagctcg
     1681 tttacaagct gatcgtaatg ctaaagcaga agaaaaaatt aaacaacttc aagaattcgt
     1741 tgcacgattt tcagcgaatg cttcaaaatc aaaacaagca acttctcgta aaaaaatgct
     1801 tgataaaatt gaacttgaag agattgtacc atcaagccgt aaatacccat tcgttaactt
     1861 taaagccgaa cgcgaaatgg gaaatgattt attgaccgtt gaaaatctct ctgtcactat
     1921 tgatggggaa aaaatccttg ataatatcag tttcattctt cgtcctggtg ataaaactgc
     1981 acttattgga caaaatgata tccaaactac tgcacttatt cgagcattga tgggggatat
     2041 cgaatatgaa ggaacaatca aatggggagt aacaactagt cgttcttacc ttccaaaaga
     2101 caattcacga gactttgctt ctggcgagtc aatcttagaa tggcttcgtc aatttgctag
     2161 taaagaagaa gacgataata ccttcttacg tggcttccta ggtcgtatgc ttttctctgg
     2221 agatgaggtt aacaaatctg ttaatgtctt atctgggggt gaaaaagtac gcgttatgct
     2281 ttcgaaatta atgctattaa aatctaatgt tcttgttctt gatgatccaa caaaccactt
     2341 agatttggaa tctatttcaa gtctcaatga tggactaaaa gatttcaaag aatctattat
     2401 ttttgcaagt catgaccacg agtttattca aactttagcg aaccatatta ttattctttc
     2461 taaaaacggt gttatcgatc gtatcgatga aacttacgat gaatttcttg aaaataccga
     2521 agtacaagct aaggtcgctc aactctggaa ataatccttt ggcatatctc taacctaata
     2581 aacgtgtcaa gattgaaaat cttaaaacta tattaatcat ttaaataaaa aggttggaac
     2641 aaatagcgtc cagccttttt atcttaggag atatatatga caattaacca ctataagctt
     2701 cgtatacctt actatacaat aagctttcta cttcccttca taatcatagt ctgtatcctc
     2761 tttactaaga atatttattg gggaagtcca acaaccatat tagcaagcga tagctttcat
     2821 caatatgtta tctttaatca agcattaaga aatattcttc atggttcaaa cagcttattc
     2881 tataccttta caagcggttt aggtttaaat ttttatgctc tgagtagtta ctatttggga
     2941 agttttttat ccccaatagt ttatttcttt aacctaaaaa atatgcctga tgctatctac
     3001 cttttaacaa tatgtaaagt agggttgata ggtttgtcaa tgtttgtcac tctttgtaaa
     3061 cgtcattgta aagttaatcg tgtcttactt ttagtaattt ctacttgcta tagcttaatg
     3121 agtttctcaa ttagccaaat tgaaattaat atgtggctag atgtatttat tctaattccc
     3181 cttgttgttt taggagttga tcagctacta tgggaaagaa aacctatact tcattttctc
     3241 tctctgacag ccttattcat ccaaaactat tattttggtt ttatgactac catctttact
     3301 agcctttact tcatcgtaca aataacccgt aatactgata gtaaggttgc atttaaacaa
     3361 tttcttcatt ttaccttttt atctctttta gctggtatga ctagtagcat tatgattttg
     3421 ccaacttatt ttgatttaac tactcatggt gaaaagttga caaaggtatc gaagatgttt
     3481 acagaaaatt cttggtacat ggatttgttt gctaaaaata tgattggagc ctatgataca
     3541 acaaaatttg gctctattcc tatgatttat gtaggtttac ttcctttact cctttcttta
     3601 ctttacttta caataaagga aattcctcga aggactagat tagcatatgg attttcacta
     3661 atatttgtta ttgctagttt ttatataaca cctctagatt tattttggca agggatgcat
     3721 gctccaaata tgtttcttca tcgctactct tgggtacttt cagtacttat ctgtctacta
     3781 gctgctgaat gccttgagta tttagacaac atttcttgga aaaagatttt aggggtcaat
     3841 ttaattttaa taagtggttt cataatcacg tttttattta aaaagcacta tcattacctt
     3901 aatcttgaat tacttctttt aaccttaaca ttcctctcag cttatattat tctaactatt
     3961 agttttgtca gtaagcaaat ccctaaatta gttttttatc cctttcttat tggttttgtt
     4021 gtgttagaga tgacattaaa caccttttac caactaaata gtcttaacga tgagtgggta
     4081 tttccatcta ggcaaggata tgctaaatat aatcacagca ttagcaaact tgtcagaaag
     4141 actgagagaa ataactctac attcttccgt acagagcgct ggttaggaca aaccggcaac
     4201 gactctatga aatataatta taatggtatt tcacaatttt catctattag aaatcgttct
     4261 tcaagtcaag tgcttgatag acttggcttt aaatcagatg gtacaaattt aaatttacgt
     4321 tatcaaaata atactttgat cgcagatagt ctatttggtg taaagtacaa ccttacagaa
     4381 tatccttttg ataaatttgg ttttatcaga aaagctcaag ataaacaaac cattctttac
     4441 gaaaatcagt ttgccagtca attagctata ctaacgaatc aagtatatca agataaacca
     4501 tttactgtga atactttgga taatcagaca actcttttaa atcaattaag tggtttgaaa
     4561 gagacgtatt ttgagcatct tattccaaat agcgtttctg ggcaaactac tcttaataaa
     4621 caagtatttg ttagaaagaa taaacaaggg aatactgaaa ttacctataa cataactatc
     4681 cctaaaaaca gtcagttata tgttagtatg ccgtttataa attttaataa tgaggaaaat
     4741 aaaattgttc aaatatccgt taataatgga ccatttgtac ctaatactct tgataatgct
     4801 tattcatttt ttaatattgg ttcctttgct gaaaacagtc gtatcaaagt aaaatttcaa
     4861 tttccacaca atgatcaagt ttctttccca attccccact tttatgggct caagttagaa
     4921 gcttatcaaa aagctatgac tgttattaat aaacgaaaag taaaagtaag aactgatcac
     4981 aataaagtta ttgctaatta tacaagtcct aatagaagtt ctcttttctt cactattccc
     5041 tatgatagag gttggaaagc ttatcaaaat aataaaggaa taaaaatttt caaagctcag
     5101 aaaggtttta tgaaaataaa tattcctaaa ggaaaaggca aagtaacatt aatttttatc
     5161 ccttacggct ttaaattt
//
LOCUS       DATRME010000106         5113 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRME010000106 DATRME010000000
VERSION     DATRME010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..111
                     /locus_tag="VBC23_001945"
     CDS             <1..111
                     /locus_tag="VBC23_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6843864.1"
                     /translation="INLVNLEKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            165..413
                     /locus_tag="VBC23_001946"
     CDS             165..413
                     /locus_tag="VBC23_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO6843865.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(497..1330)
                     /locus_tag="VBC23_001947"
     CDS             complement(497..1330)
                     /locus_tag="VBC23_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6843866.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYANYYLKQEGNI
                     KAYYFVKTAYQGENFVVGARKVDRRLIEKINKAFKQLHNKGRFQKISYKWFGEDVYSK
                     E"
     gene            complement(1344..1973)
                     /locus_tag="VBC23_001948"
     CDS             complement(1344..1973)
                     /locus_tag="VBC23_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6843867.1"
                     /translation="MLELKNISKCYEQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(1983..2624)
                     /locus_tag="VBC23_001949"
     CDS             complement(1983..2624)
                     /locus_tag="VBC23_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6843868.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(2759..3088)
                     /locus_tag="VBC23_001950"
     CDS             complement(2759..3088)
                     /locus_tag="VBC23_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6843869.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(3251..5065)
                     /gene="glmS"
                     /locus_tag="VBC23_001951"
     CDS             complement(3251..5065)
                     /gene="glmS"
                     /locus_tag="VBC23_001951"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6843870.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
BASE COUNT         1688 a         1000 c          745 g         1680 t
ORIGIN      
        1 attaatttag tcaaccttga aaaatatata gaaccaacca gaaatagggc tgattttatt
       61 attcataaag ggaaacatca taaaattgat gaaatttatc tcaaaaagtg ataaagggtt
      121 gccaaaaatc acttaattag atatacttat atagttagat taatatggag gtgaaaacat
      181 tggcaaatat taaatcagct atcaaacgcg ctgaacttaa cgttaaacaa aacgaaaaaa
      241 actcagcaca aaaatcagct atgcgtactg ctattaaagc atttgaagct aacccttcag
      301 aagaacttta ccgcgctgct tcttcaagca tcgacaaagc tgcttctaaa ggtttaattc
      361 acacaaacaa agcaagccgc gataaagcac gtcttgcaac taaacttggt taattaacct
      421 agaaaacatc ggatatatcg atgtttttta tttaattttt tcgtagaata aaagactagg
      481 agaaattcct agtcttttat tctttactat aaacatcttc accaaaccat ttgtaagaga
      541 ttttttgaaa tctcccctta ttatgaagct gtttgaaagc tttgttaatc ttttcaatta
      601 gtctacgatc aactttacga gctcctacta caaaattttc tccttgataa gcagttttaa
      661 caaaataata agcttttata tttccttctt gctttaaata atagttagca taaacttcat
      721 caatcaaaag accatcaata cggttatttt ttaaatcaat caaagcctga gtgaaagtat
      781 cgtattgaac tgcttctttt ccttttacaa acttttttaa aatatcaggt ttagcgttaa
      841 aagcatcaaa accagatgaa cccgactggg ctcctagttt tttccccttc atatccttaa
      901 tactattaat atgtgatgaa gttttagtaa caattacttg atgattattc atatatgggt
      961 ttgtaaaagc gactttttta gcacgttctg ccgtttttga ataaccattc caaataaggt
     1021 ctatattacc attattaagt tcagtttctt tcatatccca gttaataggc tgccatttca
     1081 ctgaaatacc gtattcttta aaaacagcat tagctaaatc aatatcaaag ccggtatagt
     1141 caccagaacg actttcaaat cccataggaa caaaagtatt atcaaatcca atagtaattt
     1201 tcttttcctt ttgataatgt tcccaattat caataccagc catttcctta ttagacatac
     1261 cacatgctga taatataatc ataaataatc caaatataat ggttaataat atatttttgt
     1321 gagtcaacaa tctaccccca agtctactta ggattaattt tgagaatcgt atcagatata
     1381 ctttcagcaa attgaagatc atgtgttact acaatttgtg tcatacctgt ttctctattt
     1441 tgtaaaatta gtttttctac ttcttgacgc aactctggat caagagcgct agttggctca
     1501 tcataaccaa taatctgtgg atcaatcatc atcgcacgag ctagtgcgac acgttgcttt
     1561 tgccccccag ataatgaaaa tggatagact aaagcatggt ctttaagtcc caagcgttca
     1621 agtaatacta aggctttttc tttagcaagt tcttttgata atcccatcgt tttaacagga
     1681 gagaggatca aattatctaa tactgttaaa tgtgggaata attgaaagtc ttggaagacg
     1741 aatcctaaaa gatttagtgt ttccaaatga tcaacagata cttctttacc atcgtgaaca
     1801 atagtccctg aatcaatctt ttccaatcca gctaacatac gtaatagtgt tgtttttcca
     1861 cctccagaag gtcctactaa agagagaatt ttaccttctt ctacagtcag attgaaatct
     1921 ttaaagattt ctttttgctc atagcattta gaaatatttt ttaattctaa catcgtccct
     1981 ccttatttat agtagttaaa acgcttttca acttgtttag ataaaatagt taaaagacca
     2041 attaataata ggtaaatacc acctgctata aacattggtg caagtgttgc atccctattt
     2101 gcggctgtct tactttctag aagtaagtcg cccacaccaa gtatgtaaac caatgaggaa
     2161 tctttcacta gagtaatcgt ttcattgaaa acactcggta agactatttt gaagacttga
     2221 ggtaatacta tgtaccaaac cgtttgaaac ttagaaaact ttaatacctt tgcagcttca
     2281 tattgccctt taggaataga ctcaatacct cctcgaaaaa tctctgcaaa ataagctgca
     2341 taattcatcg taaaagcaat aatcgcagct ggcatacgat caaaagaaat tcctacactt
     2401 ggtaaaaagt aataaaaaaa gataagctgt aataacagag gcgtgcctcg cataatccaa
     2461 atataagttg tcagtagcca ttgaagcggc ttaaaatgaa ctttcattag gaaggctaca
     2521 acaataccaa gtggaattga taaacaaata acaataaaga aaacttgcaa tgtcacttgc
     2581 gcacctacta ataagctagg taagatttct aaaatataag acatggtccc ccctaaataa
     2641 aaaatagatg ttctcattat atcaaaattc aaaccaatgt catagagctt ttttaataaa
     2701 aatgcatgaa atcaagtcat attcaaaaag caattgaccg tcgccaattg cctgaaagct
     2761 atttttttat aaactcagat tttagtttca tagctccaaa accatcaatt ttacaatcga
     2821 tattatgatc gccatcaact aaacgaatat ttttaacacg agtgccttgc ttaatatcct
     2881 taggcgctcc ttttactttt aaatccttaa taactgtcac agtatctcca tctgataagc
     2941 gcgttccatt gctatccaat acaattaaac ctacttcttc ttcgatttct tctggattcc
     3001 actcgtaagc acattctgga caaaccagta aaataccgtc ttcatagaca tattcagagt
     3061 tacacttagg gcaatttggt aatgacattt ttctctcctt aaattaatta ataaaatagt
     3121 cagcaagtaa cattctaact gattataggc ttcttgacaa taggaaaaag cctattttcc
     3181 ttcaaaaata ggctttttta caatttttaa tattttgtag ttaatattag cgtattttag
     3241 tgtaacgagt ctattcaaca gttacagctt ttgccaaatt acgtggttta tcaacatcta
     3301 agccacgttg taatgaagca tagtaagcaa ttaattgagt cggaataacc attgaaatgg
     3361 ctgataagaa tgggtgaact ttattaacaa taatatcatc accttcacgt tccaaccctt
     3421 cttctacaat aatcaaagca tttgctccgc gagaaacaac ttcttggata tttccacgag
     3481 tatgcgctgc aatagttgaa tcagcagata ttaaagcaat aacaggagta ttgtcttcaa
     3541 tcaacgaaat tgtaccatgt tttaattcac ctgcagcaaa accttctgtc tgaatataag
     3601 aaatctcttt tagcttcagt gcagcctcca ttgtaacata atagtcgtta ccacgaccaa
     3661 tgtagaaggc attacgtgtc gagattaaca actgctctac cttctcagag ataacatctt
     3721 tttctgataa ggtcgcttca attgattgtg ctacaattga caattcatga accaaatcaa
     3781 aatcttttgc ttctgcctta ccatttgctt ctcctaccgc ttttgctaag aaagctaacg
     3841 ttgcaacttg agcagtatat gcttttgtag aagcaacagc gatttcaggt cctgcatgaa
     3901 tgagcatcgt ataggttgct tcacgagaaa gtgttgagcc aggcacattt gttatagtta
     3961 aactagggat tcccatttcg tttgctttaa ctaaaacttg tctactatct gctgtttctc
     4021 ccgattgact aagtaaaata aacattggct tcttactcaa aagtggcatg ttgtaacccc
     4081 attctgaaga aactccaagt tcaacaggtg tatcagttaa tttttcgatc atggtcttag
     4141 cagcaaaacc agcatgatat gacgttcctg cagccaatat gtaaagacga tctgcttctt
     4201 ggactgattt tataatatca gaatccacat tcatatctcc agattcgttc gcataagttg
     4261 aaatcaactt acgcatcaca gttggttgct catcaatttc tttaagcatg tagaatggat
     4321 aagtcccctt accaatatct gataagtcaa gctctgctgt gtagctacca cgttcaatga
     4381 cattaccatc ataatcttga acttcaacgc tatctttttt aacaataacg agttctttat
     4441 cgtggatttc catatattca cttgtctcac gtatcatagc catggcatct gaacacacca
     4501 tattataacc atttcccaaa cctattagaa gaggtgattt atttttagct acgtaaatcg
     4561 tatcagcatc ttgagaatca ataagagcaa atgcgtatga tccttctata atatgtaagg
     4621 cttttttaaa ggcttcaagc actgataagt tatcttcttc aacaaagtgt tcaactaaat
     4681 gaatagctat ttctgtatca gtttcaccct tgaggttatg ttttgttaag taagtttctt
     4741 taatttgaag gtaattttca ataacaccat tgtgtaccaa tacaaaacga ccagatcctg
     4801 aagtgtgagg atgtgcattt ccttcagtag gtttaccatg ggttgcccaa cgtgtatgac
     4861 cgatacctgt cgtaccacta acagaatctc ctacttttgc ctgaagctcc gcgattcgac
     4921 cgactgactt cacaagttga cttttgttat cacctactac aaaaatacct gctgagtcat
     4981 atcctcggta ctctagcttt tctaagcctt gaattaaaat atccgttgca tttgtatttc
     5041 caactacacc tacgatacca cacatattat tattaccaat aagagctttc tcttatactt
     5101 tctatctaaa ctt
//
LOCUS       DATRME010000107         5031 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRME010000107 DATRME010000000
VERSION     DATRME010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(52..813)
                     /gene="recO"
                     /locus_tag="VBC23_001952"
     CDS             complement(52..813)
                     /gene="recO"
                     /locus_tag="VBC23_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6843871.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(803..1978)
                     /locus_tag="VBC23_001953"
     CDS             complement(803..1978)
                     /locus_tag="VBC23_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6843872.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            complement(2086..3054)
                     /locus_tag="VBC23_001954"
     CDS             complement(2086..3054)
                     /locus_tag="VBC23_001954"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6843873.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMRAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            complement(3178..4503)
                     /locus_tag="VBC23_001955"
     CDS             complement(3178..4503)
                     /locus_tag="VBC23_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6843874.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSN
                     TYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGY
                     GHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            complement(4739..4826)
                     /locus_tag="VBC23_001956"
     tRNA            complement(4739..4826)
                     /locus_tag="VBC23_001956"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4790..4792),aa:Ser,seq:gct)
     gene            complement(4840..4913)
                     /locus_tag="VBC23_001957"
     tRNA            complement(4840..4913)
                     /locus_tag="VBC23_001957"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4877..4879),aa:Ile,seq:gat)
     gene            complement(4948..5018)
                     /locus_tag="VBC23_001958"
     tRNA            complement(4948..5018)
                     /locus_tag="VBC23_001958"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4984..4986),aa:Gly,seq:tcc)
BASE COUNT         1638 a         1009 c          800 g         1584 t
ORIGIN      
        1 ctaatattaa aaaaaagaac atgacactaa tgtcatgttc ctctttacaa cttaatctga
       61 tttcataata cttccccaac tagacaaatc atcaataaat tttttacttt taagatgaat
      121 ccctacatat tcatcgtaaa tcatgtccaa aaattgacga attttaagtt tcatctcagg
      181 tttcacagaa attgtttgta aatcatcaaa ttgaattgac tgaaaacgat tgattaagta
      241 cagcatatta ggatctaggt ggtttcttct ctcgtcttta taataatggt ttggacataa
      301 taatccagaa tatttgtgtg aaaaatcaaa aggtaatcct gccctatgac aaaaaacaca
      361 atcatgaaaa tttaaagcaa caccgaacct ctctagtagt tgtacttcaa aaatattcgt
      421 tagaatctca taatctaaac cgtcctcaat caattctaac gttttcttta ggaaaataaa
      481 taattgagca tccgctacat tatcactaat agccacatca gccaaagaag taatataact
      541 cgcatatgac aacttaaaca agtctgaatt ggtttcttga aatgctaaaa cttctttata
      601 atcatcaata taagaaagac cattatcatt aatttttaaa ataaaatgag caatagtcaa
      661 aggttggagc acagcattga attttgactt tgaagcatgc tttacaaaaa acattctttt
      721 cccctcagtc tccgtaaaga ttttaactaa tttatcgtct tcacgataat tacgattata
      781 caaaacgaga ccgtatgttt gactaaccct catatgattc cataaaaact ttcaatctag
      841 ccattgctgt ttcaataact tccatactag cagcataaga aagacgtata tacccttcac
      901 catatttacc aaaagccacc cctggaataa tagctacagc ttgttgataa gcaaaatcct
      961 gacaaaactt gaaagaatcc tgtggataac tatcaggtat tttagcaaag atataaaaag
     1021 cgccgctcgg tttaattatt ttgaaaccaa gtttagacat tttatcaatg atataatctc
     1081 tacgtttaat atatccttga cgcataggta acgtatcatt tttacccaca gataaggctt
     1141 ctaccccagc aaattgcgaa atagtactag cagctgtcac catgtattga tgacttttaa
     1201 tgatttgagc agtaaatgct tcaggagcat acaccaatcc aactctccaa ccagtcatag
     1261 catgtgattt tgataagcca ttaattaaga tagtttggtt aggtaaatat tctgcaattg
     1321 aaacatgttg ctgaccagtg taagttaact cagagtaaac ttcatcacta ataacaaaaa
     1381 tatcatattt tttaaggact tcagctaatg cagcaatttc ttgccttgaa taggtaatac
     1441 ctgtaggatt agtaggataa ttaagtaaaa cagcctttaa tttttcacct tgttggataa
     1501 tagccgtttc taacatttca ggggttagtc taaagtcatt ttctctagta tcaatttcta
     1561 cgatatcagc tcctacaagg ttaactattg gctcatagcc tggataagcg ggggcaggta
     1621 atagaacaac atcaccagct tctaaaatag ctataagaga tgcagacaga gcttctgtgg
     1681 cacctatcgt aaccaagatc tcactatcag ggttataagt aagattatat ttatcctttg
     1741 caaaatctgc agcagcttgc cttagagcta ataaaccgct cataccagta taatacgatt
     1801 gattcgcatc aatagctgat ttagctgctt ctttaacatg atcaggagtg gtaaaatctg
     1861 gttctccaag agttaatttt accattccag gtatatctga aatagattga tcaaactgac
     1921 gaattaagga gacctctatt ctatctaagt acttattaaa tcgtttctct aatgtcatca
     1981 taacctcctg ataagaaata atatatttaa ttatacaaga aaaataattt gtgtccagtt
     2041 ttccgtcatt aaatgataaa aaacctgtta ataacaggtt ttttatcatt ttagtttatt
     2101 catttcaaat aatggtgata aaggacgttt ctcgtgaata cggataatag cttcaccaat
     2161 caattccgca attgaaattt gttcaatctt atcaatcaaa cgttcttcag ggaggtaaat
     2221 cgtatctaac acaattaatt tttcaatagc tgaattttga atattatcca aagccggccc
     2281 tgataaaaca gggtgagtac atgaagcata aacagctgtt gcccctgctt cagctaaagc
     2341 gtctgcagca tggcaaatag tacctgcagt atcaatcata tcatcaatta aaatacattt
     2401 tttacctttg atattaccaa taatattcat tacttcagat gtattcattt tagtaacact
     2461 acggcgctta tcaatgatag caatcggtgt ctttaaacac tgtgccagct tacgtgcacg
     2521 tgttacgcca ccatgatcag gactaacgac aacaacatca tcaccaacta atccctgacg
     2581 atcaaagtaa tctgcaataa ggggagccct cataaggtga tcaacgggaa tatcaaagaa
     2641 tccctgaatt tgagctgcat gtaaatcaac tgttaaaaga cgatcaacac cagcaacttc
     2701 tagcatgttc gcaacgagtt tcgaagtaat aggttcacga gaacgtgcct tgcgatcttg
     2761 acgagcatac ccatagtaag gcattaccac actaacagac tccgcactag cacgctttaa
     2821 agcatcaacc ataattaaaa tttccatcaa attatcatta actggtgagc ttgttgattg
     2881 taaaataaaa acatgatggc cacgaataga ctcttcaata ttaacttgaa tctcaccatc
     2941 agaaaattgg cgaactgtcg attgacctag cggaattcca atcgtctgag acactttttt
     3001 cgctagttct ttgtttgatg acagtgcaaa caattttaaa ttagaataag acatgatttc
     3061 ctccaaaact ttagtcctat accattttaa cgctttttac acaatttttc aatccaaaat
     3121 aaaccatacc taattaataa acaaaaagga gaccacgaaa gatctcctta gtattcatta
     3181 atttgggtaa atatagtaaa cagagccaga agcactaggg ttaaatgaac cacggtaatt
     3241 gccaatgctc atatttccag catagtttga ttccataact tggatagatg aattgttagc
     3301 cactgacgtt actactgcaa cgtgcccgta accaccacca tcatatggcc aaacagctac
     3361 tgcaccaaca cgaggggtag ttcctacaga ataaccagct gcacgtgcac tagcacccca
     3421 ctgattagca tttccccaat agttaccaac ccaagaagcc attgacttag ctccccaagt
     3481 acattgtccc attgggtatg tgttacttga atcataactt ctgacaacag gttttgatac
     3541 cgctctaggt gttgaagaaa caactgcttt aggtgcttca actgctttag ttactacaga
     3601 aggttgagta acttttggct cattagtagc tgtagctgta gttttagcaa cagtagttgc
     3661 cgaagaagta gccgtaagat tagaaggctt tatttcagtt ctcggttgtg tttgaactgt
     3721 ttcagtagga gctgtggcag actcaacttg tgctgctgcc tgtgcttttg ctacagactc
     3781 agcttgtgct ttcgcttcag ctactgcttg tgcttcagct gccttacgcg cagcttcctc
     3841 agcttgtgct tttgcctgaa ttaaagatgc tttttcattt tggactgtag tcaattgagc
     3901 agataactct aattgagctg cttctaattg tgctctttgt gttgctaagg ctgctttatt
     3961 attttcgata gcttgcttgt tagctgctac agtatttata gcattttgat tttcaatttg
     4021 tttagcttct aaggctgctt tgtcagcctc ttgttgtgct aacatctttt cattagctga
     4081 aacaacctca cgaatagcaa caacacgatt aacagcatca gatactgatt tcgaatttaa
     4141 aatagtatta atatagttag tcaagttacc cttttgagca ctacgaactt gctttttcaa
     4201 agattcgtta cgtgcaacaa ttttattaga aagagtttga atctcttgac ctaactgctg
     4261 agatactgcc tcaagttgag cattttgcgc ttctaattct gattgttgag actctaaagc
     4321 accaacttgt cctttaatag cagtcacctg gttttgtgct gctgcttgtt gccctgataa
     4381 agtattgatt actgaatcag tagcagcaat tttcgagtca aagtcatcag cgtttactgt
     4441 tacggcagca gttccaagag ttactccact cacaaggact gctgataata ttcttttttt
     4501 catttttatt aaactactcc tttacgataa gacattaaat attttaccaa aaaattcacg
     4561 aaattatatt acgtcattgt tacatttata tttgaaatca actatttcta aatgaaccat
     4621 aatcaaatct agaaaacgat aaccttcttc tattcactct atcaatataa ttactccata
     4681 gtgaaactaa aagagaaata aaaaaagagt ataattactc ttaaaattat aatatttacg
     4741 gagaataagg gattcgaacc cttgcgccag ttacccgacc taacgattta gcaaaccgtc
     4801 ctcttcagcc tcttgagtaa ttctccaaat taatattaat gggcacgagt ggactcgaac
     4861 caccgacctc acgcttatca ggcgtgcgct ctaaccacct gagctacgcg cccaagcaaa
     4921 tgcttggttt tacttttatg taaagtaagc gggtgacgag aatcgaactc gcgacaacag
     4981 cttggaaggc tgtagtttta ccactaaact acacccgcta aaaacttata t
//
LOCUS       DATRME010000108         5018 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRME010000108 DATRME010000000
VERSION     DATRME010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(53..126)
                     /locus_tag="VBC23_001959"
     tRNA            complement(53..126)
                     /locus_tag="VBC23_001959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Asn,seq:gtt)
     gene            complement(156..227)
                     /locus_tag="VBC23_001960"
     tRNA            complement(156..227)
                     /locus_tag="VBC23_001960"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Glu,seq:ttc)
     gene            419..492
                     /locus_tag="VBC23_001961"
     tRNA            419..492
                     /locus_tag="VBC23_001961"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Arg,seq:tct)
     gene            complement(565..1044)
                     /gene="rlmH"
                     /locus_tag="VBC23_001962"
     CDS             complement(565..1044)
                     /gene="rlmH"
                     /locus_tag="VBC23_001962"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6843875.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1245..2474
                     /locus_tag="VBC23_001963"
     CDS             1245..2474
                     /locus_tag="VBC23_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6843876.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            2572..3345
                     /locus_tag="VBC23_001964"
     CDS             2572..3345
                     /locus_tag="VBC23_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6843877.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
     gene            3554..4915
                     /gene="dnaA"
                     /locus_tag="VBC23_001965"
     CDS             3554..4915
                     /gene="dnaA"
                     /locus_tag="VBC23_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6843878.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1768 a          872 c          848 g         1530 t
ORIGIN      
        1 attttattaa caaaaaaacg ccgacaaatc aacgtttaga tgtgtcttat cactccggca
       61 gcaggactcg aacctgcgac atcatgatta acagtcatgc gctactacca actgagctat
      121 gccggataaa taaaaaactg caaagcagtt atcaatagtc cgtacgggat tcgaacccgt
      181 gttaccgccg tgaaaaggcg gtgtcttaac cccttgacca acggaccata ggttaggtgt
      241 ttccttagta ctcttaatat tatatcagaa aaaaaaggtt tgtctatact tttgttcaat
      301 aatttttaca aaaaaattga acctacttat gagcaaaaaa atatatttcc aactcataaa
      361 tagtacatat gaaaacttga cagcctgcct aatattttgg tagaataaca gagttgatgg
      421 ttccatagct cagctggata gagcattcgc cttctaagcg aacggtcgca ggttcgaatc
      481 ctgctggaat cagataatga aagcctagat agattatcta ggctttttta tttgttttta
      541 gcggttgttt cataaatgga acgattattt gtggtaagga gtgccttgac gaatcataaa
      601 agcacgataa atttgttcca tcaagactaa tttcataagt tgatgaggta aggttaacag
      661 tccaaaactc attaaataat cagcacgttt tttcactttt tgagataaac ccaaactacc
      721 accaataaca aaagtaatag tagagtagcc ggatatcatt ttttgatcga ttagatggct
      781 aaatgattca gatggcaatt gtttcccttc tatagccaaa ccaatgacat aatccctctc
      841 acctatttta ctcaaaatac gttccccttc tatatctaaa attctttgat tttcagatat
      901 actagcttta tcaggtgtct tttcatcagc tagctcaatt gtctcaattt tagaaaagcg
      961 attcagacgt ttctgatact ctgcaacacc ttctttaagg tatttctctt tcaattttcc
     1021 aactgtaatt atttttaatt tcatagctac attataacat aatccacaat attttcacaa
     1081 tttatccaca tgtttatcct tacatatccc cttattttat aggcaatcta taactataat
     1141 caaggttatc cacaacttgt ggatatttcc tttttttagc ttttaaggta taattaagaa
     1201 gcttgtcata agattagtat aaaaatttac ggaggtagct atttgtgaaa aaaaaattag
     1261 tctcatcact tctaaagtgt tctctaatca ttattgttag ctttgctggt ggagcatttg
     1321 ctagttttgt catgaatcat aatgacaata ttccaaatgg tggtgtcact aaaactagta
     1381 aagtaaatta taataacata acgcctacaa caaaagctgt taaaaaggta caaaatagtg
     1441 ttgtttctgt tatcaattat aaacaacaag agagtcgttc tgacctatca gacttctata
     1501 gtcatttttt tggtaatcag gggggcaaca ctgataaggg cttacaagtt tacggtgaag
     1561 gctctggagt catctataaa aaagatggta aaaatgccta tgttgtcact aataaccacg
     1621 tcattgatgg ggctaaacaa attgaaattc aactagctga tggctcaaaa gcagttggga
     1681 aacttgttgg gtcagatacc tactctgatt tagccgtcgt caaaattcca tcagataaag
     1741 tttcaaatat tgcagaattt gctgattcat caaaactcaa cattggtgaa actgctatag
     1801 cgatcggaag ccctcttgga actgagtatg caaattctgt aactcaaggt attgtatcta
     1861 gtttaaaaag aactgtaaca atgactaatg aagaaggaca aacagtttct acaaatgcta
     1921 tccagacgga tgctgctatc aatcctggta attcaggtgg agcacttatc aatattgaag
     1981 gacaggttat tggaattaat tctagtaaaa tttcttctac atcaaatcaa acctcaggac
     2041 aatcgtcagg aaatagcgtt gaaggtatgg gatttgccat tccttcaaat gatgttgtta
     2101 agattatcaa tcaacttgag agtaacggac aagtagagag acctgctcta ggtatttcta
     2161 tggctggatt aagtaattta ccatccgatg ttattagtaa actgaaaatc ccaagtaatg
     2221 ttactaatgg tattgtagta gcatctatcc aatctggcat gccagctcaa ggcaaactaa
     2281 agaaatacga tgtcattact aaagttgacg ataaagaagt agcatctcca agtgatttac
     2341 aaagtttact ctatggccac caggtagggg attccataac agtaaccttt tatcgtggtg
     2401 aaaataaaca aacagtcact ataaaactta ctaaaactag taaagattta gctaaacaac
     2461 gagcaaataa ctaaatcaat tctagttatt ttaactaaga tatagtaaaa ttaaaggggt
     2521 tggtcagata gaccaatccc tttagtaagt ctatagaatc tgaggtctaa aatggaatat
     2581 ttagaaacga tcaacatcaa tcatattgct ccaaatccgt atcaacctcg cctagaattt
     2641 aatacaaaag aactagaaga attagccgac tctattaaaa ttaatggcct tattcaaccc
     2701 attattgtaa gaccgtcagc agtctttggt tacgaactgg tagccggaga acgacggtta
     2761 agagccgcaa agttagctaa attggaaagt atacctgcaa ttataaaatc ctataataat
     2821 gatgatagta tgcaattagc tattgtcgaa aatttacaac gatctaattt gtcacccatt
     2881 gaagaggcta aagcctactc tcaacttcta caaaagaaaa gtatgacaca tgaagagtta
     2941 gcaaaatata tgggaaaatc tcgtccctat atatcaaata cgatacgact actaaattta
     3001 ccgccactaa ttacttcagc tattgaagaa ggaaaacttt cctctggtca tgcgcgtgca
     3061 ctactcagtc taccagatgc tagtcaacaa aaagattggt atcagagaat cctcactgaa
     3121 gatattagtg taagacgact agagaagtta ttaaaacaag aaaagaaaac aaatcataag
     3181 tcacttcaaa ataaagatgt ctttctaaaa catcaagaaa atgagttagc tcaatttcta
     3241 ggtagcaaag tgaaacttac tattaataaa gatggtgctg gcaatataaa gattgctttc
     3301 aataaccaag aagaattaaa cagaattatc aacactctaa aataagggtg ttgattattt
     3361 ttttatttaa tcaaacttat ccacaaggta ttttgctatt tttctgttga ttctctaaac
     3421 ttttctaatt tccacagtct gtggaaaact ttaattaaca ttgttgattt tattcttcaa
     3481 catctgtgga aaactttatt tttttatggt acaatataac aataattatc cacaagacaa
     3541 taaggaagaa gctatgacgg aaaacgaaca actattttgg aatagagtac tagagctatc
     3601 tcgttctcaa atagcaccag cagcttatga attttttgtt ctagaggcta gactcctcaa
     3661 aattgaacat caaactgcag ttattacttt agataacatt gaaatgaaaa agctattctg
     3721 ggaacaaaat ttggggcctg ttatcctaac agctggtttt gaaattttca atgctgaaat
     3781 tacagctaac tatgtctcaa acgatttaca tttacaagaa actagttttt ctaactacca
     3841 gcaatctagc aacgaagtaa atactttacc aattagaaaa atcgactcta accttaaaga
     3901 gaaatatact tttgctaatt ttgttcaagg agatgaaaat agatgggctg tttcagcatc
     3961 aattgctgta gctgatagtc ctggcacgac ttataatcct ctatttatct gggggggacc
     4021 tggtctagga aagacgcatc tactaaatgc tattggaaat caagttttaa gagataatcc
     4081 aaacgcgagg gttttataca tcactgctga gaattttatt aatgaatttg tcagtcatat
     4141 tcgtttagat tcgatggaag aattaaaaga aaagtttcgc aacttggact tactcctgat
     4201 tgatgatatt cagtcgcttg ctaagaaaac cttagggggg acccaagagg agttcttcaa
     4261 tactttcaat gctttacata caaacgataa acaaatcgta ttgaccagtg accgaaatcc
     4321 aaatcaatta aatgatctag aagaacgtct agtcacgcgc tttagttggg gactcccagt
     4381 aaatatcaca ccacctgatt ttgaaacacg agttgctatt ttaaccaata aaattcaaga
     4441 atatccttat gattttcctc aagataccat tgaatactta gcaggagaat ttgattccaa
     4501 cgtacgtgaa ttagaaggag ccttgaaaaa tattagtcta gttgctgact ttaagcatgc
     4561 taaaactatt acagtagata tagctgcaga agctatcaga gcacgtaaaa atgacggtcc
     4621 tattgttact gttattccta tagaagaaat tcaaatacaa gttggtaaat tctatggcgt
     4681 aactgtaaaa gagataaaag caactaaaag aacacaagat attgtccttg caagacaggt
     4741 agccatgtac ttagctcgtg agatgacaga taacagtctc cccaaaatag gtaaagaatt
     4801 tgggggacga gatcactcaa ctgttctcca cgcttataat aaaataaaaa atatggttgc
     4861 tcaagatgac aacttacgaa ttgagataga aactatcaaa aataaaatca ggtaggcttt
     4921 gtggataagt atcagaaaaa cacgagactt ttccacaggt tgtgaacaag ttattttcct
     4981 tatgaatact aaagttataa tacttattca caacttta
//
LOCUS       DATRME010000109         4593 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRME010000109 DATRME010000000
VERSION     DATRME010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            115..1614
                     /locus_tag="VBC23_001966"
     CDS             115..1614
                     /locus_tag="VBC23_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6843879.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            1616..2332
                     /locus_tag="VBC23_001967"
     CDS             1616..2332
                     /locus_tag="VBC23_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6843880.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            2432..3499
                     /locus_tag="VBC23_001968"
     CDS             2432..3499
                     /locus_tag="VBC23_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6843881.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            3511..3963
                     /locus_tag="VBC23_001969"
     CDS             3511..3963
                     /locus_tag="VBC23_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6843882.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            4265..4429
                     /locus_tag="VBC23_001970"
     CDS             4265..4429
                     /locus_tag="VBC23_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843883.1"
                     /translation="MLLTSIDVNPVYANMAFDWVYDNYKDDIINYGQETIKNIGKSIS
                     ANNSLGTLYN"
BASE COUNT         1471 a          708 c          910 g         1504 t
ORIGIN      
        1 ttttcagaaa ataatattat ttcttgatta aaaaatcaaa aaataacctt ataagaccta
       61 tttgttattc ttaaaagata ttagttaaaa tcagaaatag agattggaga taaaatgact
      121 acaaaagcaa aacatgatat tcatggcaaa ccatataatc gtacagccat gatcacctta
      181 ttgttgattg ctacatttgc aggggtatta aatcagacaa gtttgggaac ggctatccca
      241 actttgatga atagctttaa catttcgttg tcaacggctc aacaagcaac aacatggttt
      301 ttattagcga atgggattat gattcctgtt tcagcttatt tagcgacacg tttttcaaca
      361 aaatggttgt atgtgacatc ctacgttgtt ttacttattg gactgttaat gacgacgtta
      421 gcacctacat ctaactggaa ccttttttta gtaggacgta ttattcaagc gatttctgta
      481 gggatttcta tgcctttgat gcaggttgtt atggtgaatg tttttcctcc agagcagcgt
      541 ggtgccgcta tgggacttaa tggtttggtg gttggtcttg ccccagctat tgggcctaca
      601 ttagcagggt ggattttaaa acaagaattt cattttgcag ggcatgattt aacatggcgt
      661 gcaattttcc ttcttccttt actaatttta acggtcacta caattttatc cccctttgtt
      721 ctaaaagatg tggttgataa taagtcagtt aaattggaag tgccttccct tatcctttca
      781 ataattggct ttggtagctt tttgtggggc ttcacaaatg tggcaactta tggatgggga
      841 gatattggat atgttatttc tcctattatg gttggtatta tttttatcgc cttattcatt
      901 catcgtcaat taaaactaga aacaccgttc ttagatatcc gtgttttcaa aaataaacaa
      961 ttctcagtaa caacagcagc tattgcactt tcaatgatgg cgatgatggg tgttgaaatg
     1021 atgctaccgc tttacttgca aaatgttcat ggtctctctg cacttgattc tggtttagct
     1081 ttattaccag gtgccttgat gatggggata gttagtccaa tttctggagc tgtatatgat
     1141 aaagttggtg caagacgtat ggctatgatt ggttttacca tactaggtgt agcgacgtta
     1201 ccttttgttt tcttaacgac tacaacacca gatcatttta ttaccctctt atatgcagta
     1261 cgtatgtttg gtattgctat ggttatgatg ccattgacag caagtgcgat gagcgcactt
     1321 ccaccacatg aggccgcaca tggaacagca gctaataata ctgctcgtca aattgcttca
     1381 gcagttgtag ttgcattgct ttcaagtgtt gctcaaaata ttatcaccaa caataaacca
     1441 tcaaaagatt tgctaacaat gaatccttta aaatatgcaa atcagatgtt gaatgctagt
     1501 cttgatggct tccatgtttc ttttgccatt ggttttgtat ttgcggttct tggtttactg
     1561 gtatctcttt tcctgagaaa agggaaaatt attgagacgg aaaaggaggt atagcatgat
     1621 tattttgatt attgcactgg caactatttt gacatttgtc acatggatgt tgattcgtcc
     1681 aaaagctcta cggtatattc taggtcttct ctcattagct ttgttgtttg cctcagtttg
     1741 gatgttaacg gatcacttta ttaatcatac aggtatgact attgaaacta agacaaagac
     1801 acgtgagatt tatagtgcgg gtgatagtaa ggctccattt ggtctcatga tttataaaga
     1861 gattggtact cattcgagta ataatgtttt agtttatcga acagatttaa atcaaaaaga
     1921 agcaaaagct cactttgttc caaatacaaa agaagtgaca agtgctgtga aaaaatctgc
     1981 tagctaccaa ttagctgatg tgaaaaaagc cacagtaaca acaaagacta aacgttatgt
     2041 gtggaaagat agtctttcga aactacttta tggctttgca ggtgaaaatg gagaattgat
     2101 ttctcagaaa tcagttgcta aaataccacg aaaaacttgg ctagttttga ctaaagagca
     2161 gtccgaacac ttagaatcgg ctgcggctaa actaaaagaa caaatgactt cgcaagcaaa
     2221 agtaaaccca gtgaaagcta tggagataaa gacattagct aagacaaatc cagaagctta
     2281 tgctgagatg caagttaaag cattaaaaga agctcttaat ataaaagaat agtaaagtaa
     2341 aagcaactga aatgagttgc ttttcttata gctaaatgtt ttaaaagatg ataaaattaa
     2401 cctaattaag gttagaatag cgaggtttct tatgggtttt gaacaacgtc taactagatg
     2461 tcaaacagca atttcacaat tatcatgtga cgcactctta ataacaaatt taacaaatat
     2521 tttttatttg actggttttt caggaactaa tgcaacggta ctcattagtc caaaacaccg
     2581 gatttttgtt acagattcac gatatgcctt gatagcaaag aatactgtaa gagagtttga
     2641 tattatcatt agcagagaac cacttgcggc tattttgaaa attattcgcg atgacgcatt
     2701 gatagcgatt ggttttgaaa ctgatatttc ttatcatatg tacaaacata tggtggaagt
     2761 ttttgaagat tatcgcttga tagaagctcc atctgttgtt gaaaagttac gtatgattaa
     2821 agatgtaaca gaacttgcga tcatccgtca agcctgtcgt atttcggacc aagctttttt
     2881 agatgtgttg gattttataa agcctgatcg aacaactgaa ttgcaagttg ctaatttttt
     2941 agactttcgc atgagagaat taggagcaac tggtccttca tttgatttta ttgtagcatc
     3001 tggatatcga tcagcgatgc cacatggggt tgcgagtcag aagactattc aatcgggaga
     3061 aactctgact ttagattttg gttgttacta tcagcactat gttagtgata tgacacgtac
     3121 gattcatatc ggtcatgtca ctgatcagga acgcgagatt tacgatatcg ttttgaaaag
     3181 taatcaagct attattgata atgtaaaatc aggaatgaag cgttgtgatt atgattactt
     3241 ggcgcgacaa gtcattgaaa attctggata cggtaatcat tttacccacg gtatagggca
     3301 tggtatgggg cttgatgttc atgaaattcc ttattttggt aagtcagagg gagtgattgc
     3361 atctgggatg gtagtgactg acgagccagg aatatatctc gataataaat acggtgttcg
     3421 tattgaagat gatttattaa taacggaaac aggttgcgaa gtattgacat ctgcaccgaa
     3481 agaattaatt gtactttaaa ggaaaataga atgaatagac tttcttggga agattatttt
     3541 atggcaaatg cagagttgat ttctaaacgt tctacctgtg accgtgcttt tgttggagct
     3601 gttttagtca aaaataatcg catcattgct acaggttata acggaggagt ctctgagaca
     3661 gataactgta acgaagtggg acattatatg gaagatggtc actgtattag gactgttcat
     3721 gctgagatga atgcccttat tcagtgtgct aaagaaggta tttctactaa taatacagaa
     3781 atttatgtga cgcattttcc ttgtatcaat tgcaccaagg cgctcttgca agccggggtt
     3841 aagaaaatta cttataaagc taattatcgc ccacacccat ttgctattga acttatggaa
     3901 gcaaaaggag ttgcttatgt tcaacatgat gttccagagg taacactagg gatggaagag
     3961 taaagaggaa gatagagtat atgcctatct tccttgaagg ttttatcgtt taagctaagg
     4021 gccagttatg ttaaatatat tgaacgagat aattctcaaa attggtcaat tgttagtcat
     4081 gggattactg aaaatggcct aaaaaaggtg atcaccagtt aatagtcatt attcctaatg
     4141 atttttcaca gaaagtttta gatgttgata atagaatagc tgatccatct attatcaaca
     4201 tctaaaatta gtactgctgg gaatctcgaa ttgcaaaaat aatttacaaa aaagagaaaa
     4261 gatattgttg ctgacctcaa tagatgtaaa tccagtttat gctaatatgg catttgattg
     4321 ggtgtatgat aactataaag atgatatcat caattatgga caagaaacta tcaaaaatat
     4381 tggtaaatcc atttcagcta ataatagttt aggtacttta tataattaag gagcaagatt
     4441 tgaaaacgga aatagaaaca aataatctta ttaatttagg atggtttcat gataatgctg
     4501 tttattatga tatgaaaacg aaaaaattat ataaatctaa tctaaaaaaa aaatagcaca
     4561 ggtataatgc tactaactcc tattatttca act
//
LOCUS       DATRME010000110         4271 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRME010000110 DATRME010000000
VERSION     DATRME010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(14..367)
                     /locus_tag="VBC23_001971"
     CDS             complement(14..367)
                     /locus_tag="VBC23_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO6843884.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISSSLLKQNLLPYYLILISLLAIGITIKLLRTKTLFSYKRFLKLFWRSGFILTF
                     LFYLGLLVIIFIKVQ"
     gene            complement(428..1333)
                     /locus_tag="VBC23_001972"
     CDS             complement(428..1333)
                     /locus_tag="VBC23_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6843885.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
     gene            1591..2805
                     /locus_tag="VBC23_001973"
     CDS             1591..2805
                     /locus_tag="VBC23_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6843886.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
     gene            2813..4195
                     /locus_tag="VBC23_001974"
     CDS             2813..4195
                     /locus_tag="VBC23_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843887.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
BASE COUNT         1344 a          623 c          848 g         1456 t
ORIGIN      
        1 tatccaactc tttttattgt actttgataa agatgataac caataatcct aggtaaaaaa
       61 ggaaagttag aataaagcca gatctccaaa ataattttaa aaaacgttta taagaaaaaa
      121 gagtctttgt tcgtagaagc tttattgtaa ttccgattgc cagtaatgaa atgagaatca
      181 gatagtaagg taaaagattt tgttttaaca gactagatga tattaatgga attaaaaaaa
      241 caaaaatagg aaaagcaaca tctggaaaca taatgttaaa ttttttaagt ttaaatattt
      301 tgacaagaac ctgtgaaaat atagggatta aaacaagaaa taaagaagca ataattttgt
      361 atatcatagg tctattttat accgattttg gtaagaagta aactgatttt caatagaatt
      421 agcgaaacta cttggttaaa aaagctttat aaagtaggag agcaattcca gataattcaa
      481 catctttttt ataaatcata atgtactctg aaaataactt attcttatga aaatggtaga
      541 cattgcactc tttatccaaa tagcgatgaa tgagagtttg tggaacaaat gttgctccca
      601 tccctgacaa agataggcta gctgctgtag gaacggtact agttgtaatc atctgattta
      661 atgacatgtg atgaagctca tagaattgtg caattgtttt gccaattccg tactctaatg
      721 gtgttaggat aagaggttca ttgtctagaa ctgaaatagg atattcaaaa ggaataatct
      781 gtccacgttt ttcagcgttg tagagttggc tggtggtagg tatcattaaa aacaattcgt
      841 cgcggtaaat agtggttgat gcaattccat ctttataaag gataggagcc atcccaacag
      901 ctaggtcaat atcaccactc gctaccaatt gttcagaaat attttgattg ttttcgagta
      961 atttgacaga aatttctggg tgctccaagt taaatttggg aagtacattt gccagaatag
     1021 aacttgctaa gccagagtta atcccaagtg tgatttctcc ttttttatca gaacggatga
     1081 gatatagttc tttggccatt tgacgctcaa tcatatcaat ttcttttagg tagaatagat
     1141 atctctgtcc agcataagtt aaaaaggttt ggtgtccttg gctctggatg agttttattt
     1201 ctaactcatt ttctaattgc ttgatcactt tgcttaggta aggttgagaa ataaaaaggt
     1261 gtgcagcggc tttagtgatg tttgagtata gctcgatact ctctagatac attaattttt
     1321 gttgattttt caagacttta taaccagttt gatatcctta gattaataat aagttatgat
     1381 acgttatggc tataagttta ctatcatttt gagctaaaat caatctattt aatttagtag
     1441 tataacatga tatattcata actatttagt tatagaaata tctaaaaaaa attatttgta
     1501 aaaaattatt ttcaaaagta gactatataa gagcagattg ttagaataat agccttattt
     1561 taactgttca tattttaagg aggaaagcaa atggccaatg catctttgcg acatcaatta
     1621 tttgaaaaac ttgaccaaaa atgtgatcaa atggttgcta ttcgtcgcta tttacatgaa
     1681 aatcccgaat tatcatttaa agagacaaaa acagcagctt atatttcaga tttttataaa
     1741 ggtaaggact gtcatgttca gacacaattt ggtggtatga atggcgtcgt agttgatatc
     1801 tatggtgata aagcgacaga taagcccatc aaacacattg ctttgagagc cgactttgat
     1861 gctcttccaa ttcaggaaga gacagggctt tcttttgctt caaaaacagc gggcgtcatg
     1921 catgcttgtg gacatgatgc ccatacagct tatttattga ttttggctga gagcttgatt
     1981 gaattaaaat cagaattttc aggtcatatc cgtatcttac atcaaccggc tgaagaggtg
     2041 ccaccaggcg gagcaaaagc aatgattgaa gcaggatgtt tagatggcat tgatgctgtc
     2101 cttggtatcc atgttatgtc aacgatggaa gagggaacgg tacaatatca cgcaggtccc
     2161 attcaaacag ggcgtgccac ttttaaagtt attttgcaag gtaagggtgg acatggttcc
     2221 atgccacatc gtgcaaatga tacaattgtt gcagcttcta gttttgttat ggcagcgcaa
     2281 acgattgtca gtcgtcgagt taatccattt gacacagcag tagttactat cggatctttt
     2341 gatggtaaag gttctgctaa tgtgatcaaa gatagtgtaa ccttagaagg tgatgtgcgc
     2401 gtgatgtctg aagagacacg aggtgttgtt gaggaggaat ttaagcgtat tctagatgga
     2461 atagcacaaa cttatggtgt tagttatcag ttagactatc agaatgatta tcctgtttta
     2521 gttaataaca gtgaagtgac acagaaagtt gcaaatagtt taaaatctgt tgctataaaa
     2581 gaaattttag atgtcattga ttgcgaccct caaacgccgt ctgaagattt tgcctattat
     2641 gctcaaacaa tcccagcttg cttcttctat gttggagcac atgaagaggg gcaaccatat
     2701 tacccacatc atcatccaaa attccaaatt gcagaatcat cattgatggt atctgctaaa
     2761 tcaatggcaa cagcagcgct tgctatgctt gtagaaggag aatagataac atatgttaac
     2821 gacaaaggaa aatttcaaag gaaataacaa attattggtg ggaattgtac tcgctgtact
     2881 atcattttgg ttatttgcac agtctatttt aaacatggga ccagatgttc agtcatcatt
     2941 aggtatttca tctggtgcaa tggatattgg tgtctcaagt acggcattgt tttcaggttt
     3001 atttattgtt gtaactggtg gtttagcaga taaacttggt cgagttaaat ttacttttat
     3061 tggtctttgc ttaaatatta ttggttctct actaattgtt ttagctaatg gagcagtgct
     3121 atttattatg ggacgtattt ttcaaggatt ggcagcagct tttattatgc catcaacaat
     3181 ggccttggtt aaaacgtact atgatggtaa agatagacaa cgtgcagtta gtttctggtc
     3241 aatcggatcg tggggtggct caggtctatg ttcttatttt ggtggagcgg tggcaagtac
     3301 tctaggttgg cgttatgtct ttatcttttc aatcatcgca tcagtagtta gttttctatt
     3361 aattttagga accccagaaa gtaaaaatgt tggacaaaaa actcactttg attaccttgg
     3421 tttaattatt tttattattt caatgttatc gttaaatatt gggatatcta tggctcagga
     3481 acatggattg atgaacgtta ttccattgag tctttttaca gtgatgctta ttggatttgt
     3541 tctgttttat tatgttgaaa cacgtaaatc taatagtttt attgactttc atctgtttga
     3601 aaatcgattt tacttaggtg cgacaatatc aaatttctta ctgaatgcag ttgcaggaac
     3661 tctgattgtt attaatactt acatgcaaca aggtcgccag ctaacaccaa aggttgcagg
     3721 tgaaatgtct ttaggctatt tggtttgtgt actaattgct atccgagttg gtgagaaaat
     3781 tttacaacgt tttggtgccc gtaagccaat gcttttggga gctatgagta catttgttgg
     3841 gatattttta atgactttgg tcaatattca aggacctttg tatcttgtgt tagtctttgt
     3901 tggttatgct ttatttggaa caggcttggg gatctatgcg acaccatcaa cagatacagc
     3961 tatttctagt attcctaatg aaaaagtagg ttctgcatct ggaatttaca aaatggcaag
     4021 ttcactaggt ggtgcaattg gtgtagcaac ttcaattgcg atttatcatg ctttttctgg
     4081 taatgcagat tttcacaaag cagctttgtg tggattaatt ttaaacctag ttttttgtag
     4141 tctatcgatc ttatcaattc tttttgttat ccctaagaaa attgaggata aataaataac
     4201 aatacttgag attcaaaatc tcaagtattg ttgtaataat gtaaaaggaa ttgggaaaga
     4261 aatttaaaat a
//
LOCUS       DATRME010000111         4056 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRME010000111 DATRME010000000
VERSION     DATRME010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..723
                     /locus_tag="VBC23_001975"
     CDS             <1..723
                     /locus_tag="VBC23_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6843888.1"
                     /translation="IYLAKWAQKPIIENYERQKAFVENASHELRTPLAVLQNRLETLF
                     RKPNATILENSENIASSLDEVRNMRILTTNLLNLARRDDGIKPELAVIKPTLFDSIFE
                     NYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMTILFDNAIKYTDNDGSIDFTISET
                     DKYLFLEIADNGPGISEEDKVRIFDRFYRVDKARTRQQGGFGLGLSLAQQIVNSLRGN
                     ITVIDNKPRGSIFKIKLPKVKG"
     gene            812..1144
                     /locus_tag="VBC23_001976"
     CDS             812..1144
                     /locus_tag="VBC23_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6843889.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            1162..2727
                     /gene="ffh"
                     /locus_tag="VBC23_001977"
     CDS             1162..2727
                     /gene="ffh"
                     /locus_tag="VBC23_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6843890.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            2794..3480
                     /locus_tag="VBC23_001978"
     CDS             2794..3480
                     /locus_tag="VBC23_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6843891.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            3470..>4056
                     /locus_tag="VBC23_001979"
     CDS             3470..>4056
                     /locus_tag="VBC23_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6843892.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNL"
BASE COUNT         1419 a          691 c          764 g         1182 t
ORIGIN      
        1 atctacttag ctaaatgggc acaaaaaccg attatagaaa actacgaaag acagaaggct
       61 tttgtcgaaa atgctagcca cgagctgcgt actcccctag ctgtcttaca aaatcgccta
      121 gaaacgctat tcagaaaacc gaatgccacc attttagaaa atagtgaaaa tatcgcttca
      181 agtctagatg aagtacgaaa tatgcgtatt ttaaccacta atcttttaaa cttagcaaga
      241 cgtgatgatg gaatcaaacc agaattagct gttattaagc ctacactttt tgatagtatc
      301 tttgagaatt atgatctaat cgcccaagag aatggtaaaa attttactgg acataatatg
      361 atccaagata gtttcaaaac agataagaca ctgctaaaac aactcatgac tattttattt
      421 gataatgcta taaaatatac tgacaatgat ggtagcattg actttaccat atctgaaact
      481 gataagtatc tctttttaga aatcgctgat aatggaccag gaatttcaga ggaggataaa
      541 gtcagaattt ttgatcgttt ttaccgtgtt gacaaagcaa gaacacgaca acaaggaggg
      601 tttggcctcg gcctttcttt agctcaacaa atcgttaact cactgcgagg taatattaca
      661 gtaattgata ataaacctag aggcagtatt tttaagataa aactaccaaa agtaaaaggt
      721 taggccatgt tagtcctaac cttttaaatt ttaaagtggt cttgcatact tgatatagca
      781 agctgatagg aaatttggta taataaaagc tatggaaatt gaaaaaacaa atcgtatgaa
      841 tgcccttttt gagttttatg cggcattatt aacagataaa caaatgaatt atatcgagtt
      901 atactacgcc gatgactaca gccttgctga aatagctgaa gaatccggtg ttagccgaca
      961 agctgtctat gataatatca aacgaactga aaaaattctt gaggcgtacg aaatgaaact
     1021 acatatgtat tcagattata ttgtccgtag tcagattttt gatgacattc tagaaaaata
     1081 taccgatgat gcctttttac aagaaaaaat ttccattcta agtagcattg ataatagaga
     1141 ttaaatgagg agaaataatt tatggctttc gaaagtttaa cagagcgcct gcaaggagtt
     1201 tttaaaaata ttcgtggtaa gaaaaagtta tctgaaaaag atgtgcaaga agtcaccaag
     1261 gagattcgtc tagctctttt agaagctgac gttgctcttc cagttgttaa aacctttatt
     1321 aaacatgttc gtgaacgtgc tgttggacat gaaatcattg atacactaga tcctacgcaa
     1381 caaattgtta agattgtcaa tgaagagcta acagatttat taggggctga gacctctgaa
     1441 attgaaaaat cccctaaaat tccaaccatc attatgatgg ttggtctaca aggtgctggt
     1501 aaaacaactt ttgccggtaa gttagcaaac aaactaatca aagaagacaa tgctcgtcct
     1561 atgatgatag cagctgatat ttatcgacca gctgctattg atcagttaaa aacccttggt
     1621 agccagatta atgttcctgt ctttgatatg ggaactaacc attcagcagt tgagattgtc
     1681 actaaaggtt tagaacaggc tcgtgaaaat cgtaatgact atgttcttat tgatacagcc
     1741 ggacgcctac aaattgatgc aacccttatg caggaattgc atgatgtcaa agccatagct
     1801 caacctaatg aaatactcct tgtcgttgac agcatgattg gacaggaagc tgcaaatgtc
     1861 gctgaagaat ttaaccgtca gctcagcatt agtggtgttg ttctgacaaa gattgacggt
     1921 gatacacgcg gtggtgctgc cctttcagta cgcgaaatta ctggaaaacc aattaaattt
     1981 acagggacag gggaaaaaat cactgatatt gaaactttcc atcccgatcg tatggcaagc
     2041 cgtatattgg ggatgggaga cctgctaaca cttattgagc gcgctagtca agaatacgac
     2101 gaaaaacgtt caatggaatt agcggagaaa atgcgtgaaa acacctttga cttcaacgat
     2161 tttattgatc aattagatca agttcaaaat atggggccaa tggaagatct ccttaaaatg
     2221 cttccaggaa tggctaacaa tccagcaatg aaaaacttca aagtggatga aaacgaaatt
     2281 gctcgcaaaa gagccattgt gtcatcaatg actccagaag agagagaaaa tccaaatctc
     2341 ctcaatccaa gtcgtagacg tcgtattgct gctggatcag gtaatacttt cgttgatgtt
     2401 aataaattca ttaaagattt taaccaggca aaacaaatga tgcaaggcgt gatgtccggt
     2461 gatatgaata aaatgatgaa aaaaatggga attgatccta ataacctccc taaagatatg
     2521 cctggaatgg acggaatgga tatgtctaat ttagaaggta tgatggggca aaatggcatg
     2581 cctgacttat ctagtttagg tggcgatatg gacttcagcc aaatgtttgg tggtggctta
     2641 aaaggaaaag taggagcctt tgcagctaaa caatcaatga aacgtatggc taataaaatg
     2701 aagaaagcta agaagaaacg caaataaaaa tgtctattta aatagacatt tttatttttg
     2761 tctgctataa tagacaaaaa ggtggtgata tttatgtatt tagcattaat cggtgatatc
     2821 attaattcaa aacagatact tgaacgtgaa actttccaac agtcttttca gcaactaatg
     2881 accgaactat ctgatgtata tggtgaagag ctgatttctc cattcactat tacagctggt
     2941 gatgaatttc aagctttatt gaaacaatca aaaaaggtat ttcaaattat tgaccatatt
     3001 caactagctc taaaacctgt taatgtaagg ttcggcctcg gtacaggaaa cattataaca
     3061 tccatcaatt caaatgaaag tatcggtgct gatggtcctg cctactggca tgctcgctca
     3121 gctattaatc atatacatga taaaaatgat tatggaacag ttcaagtagc tatttgcctt
     3181 gatgatgaag accaaaacct tgaattaaca ctaaatagtc tcatttcagc tggtgatttt
     3241 atcaagtcaa aatggactac aaaccatttt caaatgcttg agcacttaat acttcaagat
     3301 aattatcaag aacaatttca acatcaaaag ttagcccaac tggaaaatat tgaacctagt
     3361 gcgctgacta aacgccttaa agcaagcggt ctgaagattt acttaagaac gagaacacag
     3421 gcagccgatc tattagttaa aagttgcact caaactaaag ggggaagcta tgatttctga
     3481 ttttttaagg gataatccaa tcctaacttt attattttgc gcacattttt tagctgactt
     3541 tcagtggcaa agtcaatcct tagcagatag taaatcacat agttggaggg gactctggag
     3601 acatctcttg attgttttcc tccccctagc agctttaatg atattgatac cagaaactac
     3661 tttgttaaat ttaagcatat ggggaagtca tattgttatt gatagtataa agaaattgag
     3721 ttacccttgg gtagaggaag ggcatttcca aaaagcagct tttattatag accaactagc
     3781 ccactatact tgcattatcg ttttttacca tgcgcttcct acatatttac cgcctaatca
     3841 ttggcttttg cctatcaagc actttattgt catagctctg gtttttatca ttatcaccaa
     3901 acccattaat attgtgttta aaattttctt taataaattt caagctaaag aattatcatc
     3961 attattaaca caagaaaaaa caaaaataat gaaagaaaaa tctgaagatc atgaagaaac
     4021 aattgaagga gcaggagcta tgattggtaa tttaga
//
LOCUS       DATRME010000112         3741 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRME010000112 DATRME010000000
VERSION     DATRME010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1720
                     /locus_tag="VBC23_001980"
     CDS             <1..1720
                     /locus_tag="VBC23_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO6843893.1"
                     /translation="NNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPDTL
                     KVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNITNSEVDKLASDYSFR
                     INTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSKIDNISTTGLAIALEAKEIYEAN
                     KSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVKKLLLELQNLT
                     KNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSVQK
                     AVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVDTE
                     GKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVAKP
                     VAKGTYDVKYVDTEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAG
                     AQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPEVKSDVKPEAKPE
                     AKPATKKSVNTSGNLVAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLITIV
                     LKHKKN"
     gene            1926..2465
                     /gene="nusG"
                     /locus_tag="VBC23_001981"
     CDS             1926..2465
                     /gene="nusG"
                     /locus_tag="VBC23_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6843894.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2512..3717)
                     /locus_tag="VBC23_001982"
     CDS             complement(2512..3717)
                     /locus_tag="VBC23_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6843895.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
BASE COUNT         1346 a          585 c          689 g         1121 t
ORIGIN      
        1 taataacgaa aaaaaagtaa aatacttttt aagaaaaaca gcttatggtt tggcttcaat
       61 gtcagcggcg tttatagtat gtagtggtat tgtaaatact cctacagtgt ctgctgatag
      121 tcctgataca ttaaaagtcg aaaaattagg caaattgaaa gatgtgaaat cagttcatga
      181 actcacaccc atatcaatac cgaacgaatt aaaaggtgct aaagagcaag cactttcttc
      241 aataatttca catcctaata taactaattc ggaagtagac aaactagcta gtgactatag
      301 ttttagaatt aatacatcta atgatgtgaa cgacgttaaa cgtctattaa atgaatttta
      361 taacgcagtt gcaaggaaac agttagatac aaattctgct gactaccgta gtaaaattga
      421 taatatcagt actacaggtc ttgcgatagc tcttgaggct aaagaaattt atgaagcaaa
      481 taaatctata ttacctcatc gttacaaaga ttctgttgga acttatgtga acagttttga
      541 ggaaagacga agtccaggaa aatttaatat ttggaatggt caggaaggat ttaatgcagc
      601 tcaaaaattg ttagaagatg ttaaaaaatt attacttgag ctacaaaatt taacaaaaaa
      661 taacaaacca aatattcaag tacctaaaca agcacctaca gaagctgcaa aaccagcttt
      721 gtcaccagaa gccttgacaa gattgactac atggtataat caagctaaag atctgcttaa
      781 agatgatcaa gtaaaggaca aatacgtaga tatactttca gttcaaaaag ctgttgacca
      841 agcttatgat catgtggaag agggaaaatt tattaccact gatcaagcaa atcaattagc
      901 taacaagcta cgtgatgctt tacaaagttt agaattaaaa gataaaaaag tagccaaacc
      961 agtagctaaa ggtacatacg atgttaagta tgtagacaca gaaggaaaag aagtagctaa
     1021 gtcacgtcac ttcgaaggag aagaaggcgc agcttttgtc acttcagcga aagaagtagc
     1081 gggttacaaa cttgttagaa cggaaggcgc tgtttcaaat gtcttcacag caggagcaca
     1141 agtacgtaca tatgtttacg aaaaagtagc caaaccagta gctaaaggta catacgatgt
     1201 taagtatgta gacacagaag gaaaagaagt agctaagtcg cgtcacttcg aaggagaaga
     1261 aggcgcagct tttgtcactt cagcgaaaga agtagcgggt tacaaacttg ttagaacgga
     1321 aggtgctgtt tcaaatgtct tcacagcagg agcacaagta cgtacatatg tttacgaaaa
     1381 agttaaacca gaagttaaac cagacgttaa gccagaggcc aaaccagagg ctaagccaga
     1441 agttaaacca gacgttaagc cagaggccaa accagaggct aagccagaag ttaaatcaga
     1501 cgttaagcca gaggctaagc cagaagccaa gccagcaacc aaaaaatcgg ttaatactag
     1561 cggaaacttg gtggctaaaa aagctattga aaacaaaaag tatagtaaaa aattaccatc
     1621 aacgggtgaa gccgcaagtc cactcttagc aattgtatca ctaattgtta tgttaagtgc
     1681 aggtcttatt acgatagttt taaagcataa aaaaaattaa atttattttt aaacataaat
     1741 cgaaaggtct atcctttcaa aatcttaagg caaacctcat cagaactttg ccttttttat
     1801 ttgaatttta gaatgattta gtcggtactt taattgacag cttattttat tttctttata
     1861 atatatattg aaaatagcag tccaataagc cgtaaaaggc ttttttatta aggaaaggaa
     1921 aacttatgtt agattctttt gataagggtt ggtttgtact ccaaacctac tcaggttatg
     1981 aaaataaagt aaaggaaaat ctcttgcaac gtgcgcaaac ttataatatg ttagacaaca
     2041 ttttgcgtgt ggaaatccct actcaaactg taaatgttga gaaaaatggg aaaacaaaag
     2101 aaattgaaga aaatcgcttc ccaggttatg tacttgtgga aatggtaatg actgatgaag
     2161 cctggtttgt tgttcgtaat actccaaacg ttactggatt tgttggatca cacggcaatc
     2221 gctctaaacc aacaccgttg ttagaagaag agattcgttc aatactcatc tcaatgggac
     2281 aaaccgttga tgtctttgat actaatatca aggaaggtga tgttgttcaa atcattgatg
     2341 gtgctttcat cggacaagaa ggacgtgtag ttgagattga gaataacaaa gttaaattaa
     2401 tgatcaacat gttcggctca gaaacacaag cagaacttga gttgtaccaa gttgcagaat
     2461 tataaaactt ctgaaaaatc catcttaaaa taaagatgga tttttgttaa tttataattg
     2521 atttaggttt aattcgttaa ttaaaacaac catattatca atagaattat gattaacgat
     2581 atgtttggtt atattctctt gatgagacgt gttgttgaaa gaataaattg gcttttgttt
     2641 caaatgaact ctttgtaaaa tattacctac ttctggacca tgattaatat caaggtatgc
     2701 agtggctaat gttattaatt tttcaattct tgcctcaatt aattcagggt aaactaggac
     2761 atttggatat tgttctagtt tgctaagttt atttgacact gttgtgtagg caccaatatg
     2821 gaattgaaca gtaggacatc tacaaattag cgattcaatg tgttccaaat tatcggaaga
     2881 tgtaagggtg aaaagatgaa tatctgaaac agaaagcaat tccatggact tttcgtaaga
     2941 aatcactttt ctttgcttgc cggttaattg gcaaaaatca agttggaagt aataccacca
     3001 tagttctcta aaccttgcaa cgctattttc attccaaggt ttgtcactag ttaaaaaatg
     3061 tataatatta ggagttctat tttctatgtt tagatataaa taagatcgat gattatagcg
     3121 gctaacgaca tcataagttt gaaagttcca tgttttgttc agagataacc aacgattatg
     3181 gcaaacaatg ttcaagatac tttgatcccc tgttttgaca actcggtgga gttcttcagc
     3241 tgtttttagt aatgttttag tgaggccatc ttcccgccat agttctagat tagctaaaag
     3301 catgccagca ttaaaccctc cttcttcatt tttatgggga tctttgacgg ctgctaagta
     3361 atgaccttta aaatctagtt caaataagta atcaagatta gtgttaacga taatatctga
     3421 atctaaataa agtacacgtg aactaggtat ataatctgct aaaaataatc taaaccatgt
     3481 actaacagtt agaaaagtcg ttagatgagc aatatcatag ttatatattt taacattaat
     3541 cagttttgat cctagaggct ctagtttcct gtttatatta cgtacccatt caactggggt
     3601 atcttgattg aataaataaa aagttatatt tctattataa acacaaatag actttattgt
     3661 tactaaggct ttatccaagt agtttgaatc aactgcaagt gcaactgctt tttccattta
     3721 aattctccta gttaagtgtt t
//
LOCUS       DATRME010000113         3727 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRME010000113 DATRME010000000
VERSION     DATRME010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            22..513
                     /locus_tag="VBC23_001983"
     CDS             22..513
                     /locus_tag="VBC23_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEO6843896.1"
                     /translation="MSILDDYEKELLVISHQIIAYLIKSGVNPSQAQDVTQDVFLQIL
                     ESDFSLPLDKIKAWMYRTTIRRYIDLYRRNKRYNEILQKEFFIENNLYYTESEKFFEL
                     HEAILHLKKDYQTLIDLYYFNDFSIKEISEITGQSQSTIKIRLMRARREVKKYLEKDN
                     FKI"
     gene            510..1457
                     /locus_tag="VBC23_001984"
     CDS             510..1457
                     /locus_tag="VBC23_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HEO6843897.1"
                     /translation="MIKTKFSDPLEQLAKKRKRKTLFKTVVLALITVLLVVGIAFKGF
                     TYLTSKNGQKTYHNFELLSEIAYPNISYDSLYYQPSGQFTGKVHADRFKDIDGVQIPY
                     SSFEENYSISGTFGTNADEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDL
                     KYVNKLNNDLIEIAITFDKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQF
                     GTDPETLNTPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSG
                     IILTGKAENFKQLKEKEWIHASSIGANIEYKPYYKLDKE"
     gene            complement(1717..2397)
                     /locus_tag="VBC23_001985"
     CDS             complement(1717..2397)
                     /locus_tag="VBC23_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843898.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
     gene            complement(2472..2881)
                     /locus_tag="VBC23_001986"
                     /pseudo
     CDS             complement(2472..2881)
                     /locus_tag="VBC23_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3123..3683)
                     /locus_tag="VBC23_001987"
     CDS             complement(3123..3683)
                     /locus_tag="VBC23_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843899.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFENGKWTDKLIYSINLHNNNLE"
BASE COUNT         1382 a          608 c          456 g         1281 t
ORIGIN      
        1 ataatagcga aaggaggtaa aatgtcaatt ttagatgact atgaaaaaga attactagtt
       61 atttcacatc aaataatagc ttaccttatt aagtcaggtg ttaatcctag tcaagcacag
      121 gatgttactc aagacgtctt tttacaaata ctagaatctg atttctccct tccacttgat
      181 aaaataaaag cttggatgta tcgtactaca attaggaggt atattgatct ttatcgaaga
      241 aataaacgct ataacgaaat cctgcaaaaa gaattcttta tagaaaataa cttgtattac
      301 accgaatcag agaagttttt tgaattacat gaagcaatat tacacttgaa gaaggattat
      361 caaactttaa tagatttata ttattttaat gatttttcaa ttaaagaaat atctgaaatt
      421 actggacaat cacaaagtac aattaaaata agacttatgc gagctagaag agaagttaaa
      481 aaatatttag aaaaggataa ttttaaaata tgataaaaac aaagtttagt gatccactgg
      541 aacaattagc caaaaaaagg aaaaggaaaa cattattcaa gacagtagta ttggcattaa
      601 taactgtact attagtagtt ggaatagctt ttaaaggatt tacatattta acttcaaaaa
      661 atggtcaaaa aacatatcat aactttgaac tactttctga aatagcatat ccaaatattt
      721 cctatgatag tctctattac cagccaagcg gtcagtttac tggtaaagta catgctgata
      781 gatttaaaga tatcgatggg gttcaaattc catattctag ttttgaagaa aattatagta
      841 tctcaggtac ttttggcaca aatgctgatg aaaaatcaga aaagaatggc ttgtatgatc
      901 gaggtactag acaaaaagtt ccacaatttt tcaataaaaa tgttaagtat aaaaaaggag
      961 aagccaaaac aactccaaca aatgatttaa aatatgtaaa taaattgaat aatgatctta
     1021 ttgaaattgc aataactttt gataagtcct atagttacaa agaaataaaa acaatgatac
     1081 caaataatat aaaacaaaat tggttatgga taggtacaag cacagaatta gatacatcat
     1141 attggccaac aaagtatcaa ttcggtacag atcctgaaac tttgaataca ccacaaatat
     1201 tcatagataa aataaaagaa aacttaaaag gtaactcaaa agtatacttt aataatataa
     1261 atatctactc agatttagaa aagtatgcca aaaaatacga caaggtaaat aatagcgaca
     1321 aattaaaatt ttcgggaata atattaacag gtaaagccga aaactttaaa caacttaaag
     1381 aaaaagaatg gatacatgct tcaagtatag gggcaaacat agaatataag ccctattata
     1441 aattagataa agaatgataa ttaattgtta aaaaatttgt ttggtaattt tacgtaactt
     1501 ttacagctaa taactcatag aaaaaaagac aagataataa tcttgacttt tttctttgat
     1561 gcccgcgacc aaagtcgagc tagcacgtcc gtgaggacac ccgattttga gtcctaccat
     1621 gcacctttaa aagttaataa acatttattt aacaaagttt aagatgttag ctaaaaaaac
     1681 atgtaatttt gaaattttag tgattaatga atgactctat tttgtcttct gctcaatgct
     1741 tgatagtgat ctgatttgat actcatttac atcaaagttt gaatcatcta gccatctttc
     1801 aaaccgtgtt tttttctctg gccattcttt atctaaaata gaataccaat ttgtatctct
     1861 gttacgaccc ttatatacaa cagcttgtct aaaagttcct tcaaaagtaa aaccaagtcg
     1921 ttttgcagaa ttattagatg gtgcattcaa actatcacat ttccactcat aacggcggta
     1981 acagagttct tcaaaaacat atttcataac caaatattga gcttctgttg ctatccttgt
     2041 ttgttttagt tttgatgaat aaaccaccca tcccatttca acaactctat tttttgtatc
     2101 aatacgcatt aatgaaaaag tccctaatgc tttaccagta ttattatcaa caatagctaa
     2161 ataataagga tcttctgatt ttaatagagt ttggaaaaag acatcaaatt cattcttact
     2221 attaaatttg ctaaaagaca tataagttaa actatcttca gtagacaatt ctccataaac
     2281 ttgaaataag tcttcaaaat gatctggatt tattttttct attctaactg ttttcccatt
     2341 aagtgtatca atcttcggat ttttaccttc cgtaaaattt tcaacgacat cacccactag
     2401 ttgattaaac ctattgtatc tcatttaatt tttctgcttt cacaaatttt tattgtcgtc
     2461 tatttaaaat attattcctt taaaatagaa taatatgctc tggttttcca ctcacctttt
     2521 ggattttttt acaatatgac gacgttcacc ttctttttgc attcttaggc attccataac
     2581 attttcagat tttttattat tagttcaaat cgagcataaa ttctttccaa atctagttgt
     2641 tcaaatccaa ttgacatcaa ttttgaacaa gcttttatca tataaccttt tccactataa
     2701 tttttattta atatataacc gaattctgct ggaaactcgt ctctaatttc taattctata
     2761 ataccaatca ttttatttga ttctgtttca atcatcgcat agattcctaa taatgatttc
     2821 atacagacat tcgccaaaat caatgtgctt cttctgtggt attggcttta tatcgttgca
     2881 tatgaaatac agtttcaaaa tcagatgaaa attctaaaaa ctcatttaca ttagataaat
     2941 caacaagctg aagtcgctac ctttccatta gtactatgat tgcacattgc taaattacaa
     3001 tatagtgtat ccaaattatt catttctaat caatattcct tccttaatta tctataaacc
     3061 acttcaataa tctccaaaaa tacaatatta taagctttaa atttctttgt aagtctataa
     3121 aattattcta aattattatt atgtaaattt atactataaa tcagtttatc tgtccatttt
     3181 ccattctcaa atgaaaaatc ttttcgagtt gtctcatagg ttaatcccaa tcttctaacc
     3241 aacttttccg atcgttcatt cccaggagta atctgtgctt caatatgatg aaaacccaat
     3301 ttttcataag tactattaag taatgccaag attgactcaa atgcatagcc attactccaa
     3361 aattgattat gaaaaacata tcccaattct ccccattgca tatctgctct tgcaagggta
     3421 acaatattta acattcctac gtgctcaccg ctttttagaa aaacaccaaa tatatatact
     3481 atgtcacttt cccattcttt cctttgtaaa tttactaaat ttttaaacca attaatgtca
     3541 cacatagaca tatcaagtat cccatcatca aatggagtta aagatggtcc acgattcata
     3601 aaactagtat accaactatc ataatcactt tcttctagtg gccttataat aagtctctct
     3661 gattctaaaa aaaatttatt caatttttat ttcctttctt ccagagtaaa taaataagtg
     3721 tgtttta
//
LOCUS       DATRME010000114         3684 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRME010000114 DATRME010000000
VERSION     DATRME010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(3..1022)
                     /locus_tag="VBC23_001988"
     CDS             complement(3..1022)
                     /locus_tag="VBC23_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6843900.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            1126..2253
                     /locus_tag="VBC23_001989"
     CDS             1126..2253
                     /locus_tag="VBC23_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6843901.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            complement(2290..3276)
                     /locus_tag="VBC23_001990"
     CDS             complement(2290..3276)
                     /locus_tag="VBC23_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6843902.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            3439..3684
                     /locus_tag="VBC23_001991"
                     /pseudo
     CDS             3439..3684
                     /locus_tag="VBC23_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1266 a          757 c          541 g         1120 t
ORIGIN      
        1 tattattttc taaaaatcgc tttagatatg atataggtca ttataattga caaaagaatt
       61 gtggtagcta atgctaacag ttgaactggc atatgtgata atttaaagag accaactcct
      121 aaacttccca acaatccccc aataccattc attccaaagg aggaaacgac atcatcaatg
      181 gcaaaagctt tcgcaacgta atccgttaca aagtaggtga agagggatgc aaagaatgtt
      241 acagctaata attgccaagt taagagatac ccaactcctg ctgtactcgt cacaagacca
      301 acaataatgc cattcaataa gcttcctgtt ttctcttcct caccattcca cttagcagcc
      361 aatgtccacg ccaaaccacc acctataata gcaaatatag tatttaaaag tatcataatg
      421 gcttgtgaat tccactcacc aactggaccc atattaaatc caaaccaacc aaatgtaata
      481 agtatcattc ctaaaaagag actatcattt ttaaccttat tgtgctgatg ttcactcttg
      541 ccaataacat gagctaaaat atacgaagaa agccctgcag ataaatgaac gaccattccg
      601 cctgaaaaat caagtactcc catctttgca aaaacaccct ctgaattcca aattaaataa
      661 gctaagggcg tatacaccaa gaaaagccaa gaaacaacag ctaataaaag tcgcttcgtc
      721 tgaactctat caataattga cccaataagc attactactg cgtaaagggc gaaacaaagt
      781 tgaaataaac aatttacaat ttgtttacca tctgcttctt ggaattgaaa gacagtttta
      841 aaatgacctt caaatgctaa aaaataggca cccatgaacc aagctgttgt cgttagcaaa
      901 acagtaagta caaactgata aataatcctt gaagtcaatg attgatgtag cgacccaaag
      961 taatagaatg caaccccaaa aatcatgagc cacatcgata aaatacataa tagtaaaaac
     1021 acaaatagtc ctttctttac aaacactctt ttatttgtta aactgtattg ttgttaactg
     1081 gttaagtatt ttatcttaca catttatttt tgtcaaggag aagacatgaa acattatcaa
     1141 accataatta tcggtgcagg cgctgcagga atcggttttg gttctgccat gcaacgatta
     1201 ggacttacaa attttcttat cattgaaaaa gggcacattg gagaatcttt tttaagatgg
     1261 cccagaacaa cacaatttat cacaccttcc ttcactacca atggctttgg ttttccagat
     1321 ctaaacgctg ttattccaga tacttctcct gctttctcat ttgaaaaaga acatctttct
     1381 ggagtggaat atgcacgcta cttacaatta gttgctgctc attataatct ccctatccaa
     1441 aacgaaacat ctgttctatc tattgataaa cgagatagcc tttttgttat taaaacttca
     1501 aagggagatt tttctgctga ttatcttatt atggcaacag gagaatttca aaatccaaat
     1561 accattgata ttaaaggtgc agatctagga atgcactatg gtcaagttga taatttccat
     1621 atcaaatctg acaatccatt tatcatcatt ggtggaaacg aaagtgcttg tgatgcacta
     1681 acacatctag tatatttggg aaatcaagtc gaattatata cagatacgtt tggtcgtaaa
     1741 gagagcaatc ccgatccaag catctcttta tcccccctaa ctaaagaacg tttaaaacat
     1801 atacaagacc acaaaaaaga atattatagt atctctgaag gcaagaaagc cattgagatt
     1861 aaacaaattg gaaaacaata tcaagttact tttgatgacg gtagtactgc ggagtctttc
     1921 cataaaccta ttctttccac aggttttcta aatacctgtc atctcattga cggaatcgct
     1981 ttatttgaat acgataaaaa tcagttgcca atagttactg aagatgacga atctactatc
     2041 gttaataact gtttcttaat tggtccaagc ttacgacaaa gagatactat tttctgctat
     2101 atttataaat tcagacagcg ctttgtccct gttattaatc atatcgcaca acgagaaggg
     2161 attatattag acgaagatac ccttgaattc tttaaagaaa atcaaatgta ccttgataac
     2221 ctagcttgtt gcgatgtcaa ttgtgattgc taaaaaatcg ctagctttat tgctagcgat
     2281 tttttaatat taaatatttg ctacttgagg taaggattct ccaatagctt ctaaacgttc
     2341 tgcccattct tgacgtgata aaccattttc ttgcacatag cgattacgtt catgtgcacg
     2401 acattcaggc gagcaccctc gaacatactt cgcttcgttt tcttctgaag caaaaatttg
     2461 tttgttacaa aacggatttg cacaattgac ataacgttca caaggtgttc catcaaaata
     2521 atctttacta ataacagttg gattaacatg attaattggc accgagatac gatcatcaaa
     2581 aacatacatc gcaccatccc aaagttcacc ttgaacttcc ggatcttttc catatgttgc
     2641 aataccaccg tgtaattgtc caacatcttt aaaaccttct cgaaccatcc aacccgagaa
     2701 tttctcacaa cgaacaccac cggtacagta cacaacaaca cgtttttcca taaacttatc
     2761 tttgttgtca cgtacccatt gtggtaactc acggaaattg cgaatatcag gacgaattgc
     2821 tccacggaag tgacctaagt catactcata atcattacgc gtatctaaaa caacagtatc
     2881 ttcatcaagc aaggcttctt taaattgttt aggatttaaa tactcacctg ttgtttccaa
     2941 aggattaata tcgctatcaa agttattatc ttctaaccca aggtgcacaa tctctttctt
     3001 ataacgaaca aacattttac ggaaagcttg ttgattttcc tcgtcaatct tgaaccaaag
     3061 atcagcaaaa cgctcatcgc tgtggatcca atccatatac ttctgagttg tttcataatc
     3121 tccagaaaca gttccattaa tcccctcatc cgcaatcaaa atacgacctt taagcccaat
     3181 agatttacaa aattctagat gtttcgcagc gtactcttcc gcattttcaa ttgaaacata
     3241 tttataatag agtaaaactc tgattttttc tgacattttt tctcctcatc tgcaaaataa
     3301 gttagtatac ttcattatat aacgactcct tacaacatag caaaaaatac gcttaggaca
     3361 tattctttgc tttaaagaca aattattctt cttaagaaca cacatatgtt aaaatagtaa
     3421 aagactagga ggacttttat gacttatgaa ttatgtctag aatacggtac ttatccactt
     3481 agacctgtag atgcttgggc tgatgaaatc aatactgcac ctgcgtttat tactgaagat
     3541 aaaaaaactt ttagaacttt tagaagaagt caatacctta ttccatgagc tctttttaac
     3601 gatagaatgt tccttccact atattggtca tgacttccca gaaaaaagag caaaaatcac
     3661 acaaatttat catgtgatta tcga
//
LOCUS       DATRME010000115         3585 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRME010000115 DATRME010000000
VERSION     DATRME010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..232
                     /locus_tag="VBC23_001992"
     rRNA            <1..232
                     /locus_tag="VBC23_001992"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            287..359
                     /locus_tag="VBC23_001993"
     tRNA            287..359
                     /locus_tag="VBC23_001993"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Ala,seq:tgc)
     gene            514..3416
                     /locus_tag="VBC23_001994"
     rRNA            514..3416
                     /locus_tag="VBC23_001994"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3491..>3585
                     /gene="rrf"
                     /locus_tag="VBC23_001995"
     rRNA            3491..>3585
                     /gene="rrf"
                     /locus_tag="VBC23_001995"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1024 a          722 c         1023 g          816 t
ORIGIN      
        1 ggctgcaact cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg
       61 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc
      121 gaagtcggtg aggtaacctt ttaggagcca gccgcctaag gtgggataga tgattggggt
      181 gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggat
      241 aaggaaacct gccatttgcg tcttgtttag ttttgagagg tcttgtgggg ccttagctca
      301 gctgggagag cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc taggctccat
      361 tgaatcgaaa ggttcaaatt gttcattgaa aattgaatat ctatatcaaa ttccacgatc
      421 tagaaataga ttgtagaaag taacaagaaa ataaaccgaa aacgctgtga atatttaatg
      481 agttttctag ttttaaagaa actagtttaa taaggttaag ttaataaggg cgcacggtgg
      541 atgccttggc actagaagcc gaagaaggac gtgactaacg acgaaatgct ttggggagct
      601 gtaagtaagc gctgatccag agatgtccga atgggggaac ccactagtta atgactagta
      661 tccatatctg ttaaggatat gagaaggaag acgcagtgaa ctgaaacatc taagtagctg
      721 caggaagaga aagcaaaagc gattgcctta gtagcggcga gcgaaacggc aggagggcaa
      781 accgaagtgt ttacacttcg gggttgtagg actgcgatat aggattaatc attatagaag
      841 aattacctgg gaaggtaagc caaagagagt aacagcctcg tatttaaaat gatagattaa
      901 ccttagcagt atcctgagta cggcgagaca cgcgaaatct cgtcggaatc tgggaggacc
      961 atctcccaac cctaaatact ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg
     1021 tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt
     1081 cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgatatg
     1141 atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtcttaat agggcgccat
     1201 agtatcatgt tgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgaggt
     1261 aaaactcact ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta
     1321 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
     1381 agcgtcgatg ttaagtctct tggaggtaga gcactgtttg ggtgaggggt ccatcccgga
     1441 ttaccaatct cagataaact ccgaatgcca acgagatata atcggcagtc agactgcgag
     1501 tgctaagatc cgtagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaatat
     1561 atgttaagtg gaaaaggatg tggggttgca cagacaacta ggatgttagc ttagaagcag
     1621 ctattcattc aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1681 ccggggctaa aacatattac cgaagctgtg gatgacacaa aagtgtcatg gtaggagagc
     1741 gttctatgtg tgaagaaggt gtaccgtgag gagcgctgga acgcatagaa gtgagaatgc
     1801 cggtatgagt agcgcaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg
     1861 ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga
     1921 tggacaacag gttgatattc ctgtactaga gtatatagtg atggagggac gcagtaggct
     1981 aactaaacca gacgattgga agtgtctggt caaacagtga ggtgtgatat gagtcaaatg
     2041 cttatatctt taacattgag ctgtgacgag gagcgaagtt tagtagcgaa gttagtgatg
     2101 tcacactgcc aagaaaagct tctagcgata catatactct acccgtaccg caaaccgaca
     2161 caggtagtcg aggcgagtag cctcaggtga gcgagagaac tctcgttaag gaactcggca
     2221 aaatggcccc gtaacttcgg gagaaggggc gctgatttta gatcagccgc agtgaatagg
     2281 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
     2341 ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga
     2401 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
     2461 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
     2521 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
     2581 ccatggagct ttactgcagt ttgatattga gtatctgtac cacatgtaca ggataggtag
     2641 gagcctatga taacgggacg ccagtttcgt ttgaggcgtt gttgggatac tacccttgtg
     2701 ttatggctac tctaacccag ataggcaatc cctatcggag acagtgtctg acgggcagtt
     2761 tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagactgg
     2821 ttggaaatca gtcgtagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
     2881 gagcagggac gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc
     2941 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
     3001 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
     3061 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
     3121 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
     3181 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
     3241 atgtagggaa gggataagcg ctgaaagcat ctaagtgcga agcccacctc aagatgagat
     3301 ttcccatgat tttatatcag taagagccct gagagatgat caggtagata ggttaggagt
     3361 gtaattgtgg tgacacattt agcggactaa tactaatagc tcgaggactt atccaaaaag
     3421 agatattgac aacgttacgg tttcttgtta gactatagat attcaatttt gagtgaataa
     3481 gactcaatag ttaagtgata attgcctagg agatacacct gttctcatgc cgaacacaga
     3541 agttaagccc tagaacgccg gaagtagttg ggggttgccc cctgt
//
LOCUS       DATRME010000116         3431 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRME010000116 DATRME010000000
VERSION     DATRME010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1264
                     /locus_tag="VBC23_001996"
     CDS             <1..1264
                     /locus_tag="VBC23_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6843903.1"
                     /translation="LFLCIKNKARIFTTIILVLSSIFVATTLYGFKSTIDLTNNLNKT
                     ASYSEIEMSVVVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEKGISITPQKT
                     DSYQDAYNRIKNGDSQAMVLNNAYVSLIELSTPDFKSQIKTIYTYKIKKKINRKNTNH
                     KEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGN
                     QYDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFT
                     SKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSV
                     TKLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAM
                     PGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            1270..2001
                     /locus_tag="VBC23_001997"
     CDS             1270..2001
                     /locus_tag="VBC23_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO6843904.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            2010..2702
                     /locus_tag="VBC23_001998"
     CDS             2010..2702
                     /locus_tag="VBC23_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEO6843905.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            2713..3402
                     /locus_tag="VBC23_001999"
     CDS             2713..3402
                     /locus_tag="VBC23_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6843906.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
BASE COUNT         1266 a          503 c          583 g         1079 t
ORIGIN      
        1 tttatttctt tgtattaaga ataaagctag aatttttaca actataattt tagtactatc
       61 ctctattttc gttgctacta ctttatatgg atttaagtca accattgatt tgacaaataa
      121 tctaaataaa actgcttcat actctgaaat tgagatgagt gtagttgtac caaaagattc
      181 taaaataacc aatatagaag ctgtcagcaa attagccgca ccagttaaaa acgatacttc
      241 aaatattact gatttgatag aacatataaa atcagaaaaa ggaatctcta ttacaccaca
      301 aaaaacagat tcttaccagg atgcatacaa tagaattaaa aatggtgata gtcaagctat
      361 ggttttaaat aatgcttatg ttagcttaat tgaacttagt acccctgatt ttaaatcgca
      421 gataaaaacg atttatactt acaaaattaa gaaaaaaatt aatcgtaaaa atactaatca
      481 taaagaaggg gtatttaata tctatattag cggtattgat acttttggct ctatatcaac
      541 agtatcaaga tctgatgtaa atattattat gacggttaat accaataccc acaaagtatt
      601 gttaacgaca acaccacgag atgcctatgt aaaaattcca gatggtgggg gcaatcaata
      661 tgataaatta acccatgcag gtttgtatgg tgttgagaca tcaatgaaaa cacttgaaaa
      721 cctttacgac atcaaccttg attattatgc tagaattaat ttttcatcat ttttaaaatt
      781 aatagacctc ttgggaggag tgacagttta taacgatcaa gcttttacaa gtaaacatgg
      841 taattttgac tttcctgttg gtcaagtaac attgaattct gagcaggctt tgggctttgt
      901 tagagaacgt tattctctac aaggaggcga taacgataga ggtagaaatc aagaaaaagt
      961 gattgcagct attataaata agttagcttc tagtcagtca gtaacaaaat taaatagcat
     1021 tacctcacag ctccaaacgt ccgttcaaac taatatgact attgataata ttaatgattt
     1081 gattaacaat caattgtcaa ctggacaacg cttcactgtc gagtcacaag cattaactgg
     1141 tcatggttca acgggtgaac tcccttcata tgcaatgcca ggagctcaac tttatatgat
     1201 gtcaattgat caatctagct tatctaatgc aaaatcaaaa attaagaaca caatggagga
     1261 ataacaagaa tgattgatat tcattctcat atcgtttttg atgtagatga tgggcccaaa
     1321 acgcttgaag agagtttatc tttaatagaa gaaagttatc gacaaggtgt tagaataatt
     1381 gtttcgacat cacatagaag aaaaggtatg tttgaaacac ctgaagatat tatcttcaaa
     1441 aacttttcta tagttaaaca cgaagcggaa aaaagatttg aacatcttca aatcttatat
     1501 ggtggagaat tatactatac aagtgatatg ttagaaaaac tgaagttaaa gcaaattcca
     1561 actttaaaca atacgaaatt tgctttaatt gaattttcta tgcaaacttc ttggaaagat
     1621 attcatacag ctttgtcaaa tgttttaatg cttggtatta caccagtcgt tgcgcatata
     1681 gagaggtata acgctttaga gaatcaaaaa gaacgggtga aggaaattat taatatgggg
     1741 tgttacacac aaataaatag tttccatatt ttgaaacaaa aactttttaa tgataagcat
     1801 aaacgcttta agaaaagagc ccgttatttt ttagaggaaa atttagtgca ttttgtagcg
     1861 agtgatatgc ataaccttga tgttagaccg ccatttttag cagaagctta taagattatc
     1921 tgtagagatt tcggtaaaga acgtgctaac caacttttta ttgaaaatgc tcaatctata
     1981 ttaaaaaacc attacattta ggagatttca tgaataaaat agctaataca gaagttgaaa
     2041 taaatatatt caacctttta aaaaaattgt ggaaaaagaa atttttaatc acatttgttg
     2101 ccattgcgtt tgcaacagct ggacttttct atagtctttt tattgttacg ccacaatata
     2161 cttcttcaac aaggatatat gtcattaacc ctaatacccc taataatagt attactgcac
     2221 aagatctaca agcggggagt tttcttgcaa atgactataa ggagattatt acgtctactg
     2281 acgttctaga aaaagttatt tcttctgaaa aattgaatta tccttcgtct cagttgctac
     2341 aaaaaataac agtttctatt ttaaaagata cacgtgttat ttcaatatcg gtcgaagatg
     2401 ctaatccaaa aatgtctcaa aaattagcaa attcagttag agaagcagca gtttcaaaaa
     2461 tcaaggcagt tactcaagta gaggatatca ctactcttga gaagggaaat ttacctaaag
     2521 caccatcttc tcctaatatt aaaaagaatg tactaatcgg gtttattgtt ggtgcaggat
     2581 tatcaactat tgttttagtt attatgggga ttttggatga tcgtgttaat actgaagaag
     2641 atattgaaaa ggtcttagga cttacttctt taggtatagt accagatttg aataaacttt
     2701 aaggagaata taatgactcg tttagaaata gttgatagca agttaagaca agcaaaaaaa
     2761 acagaagaat acttcaatgc gatccgtaca aacatacagt ttagtggaaa ggaaaataaa
     2821 attcttgcaa ttgcctctgt tagggaaggg gaaggaaaat ccactacttc aacaagttta
     2881 gctttatctt tagctcaagc aggatttaaa acattattaa ttgatgcgga tactaggaac
     2941 tctgttatgt ctggaacctt taaagcaact ggaactatta aaggcttgac gaattattta
     3001 tcaggtaatg cagatcttgg agatattatc tgtgaaacca atgttcctag actgatggtc
     3061 gttccttcag gaaaagtacc accaaatcca acagcattac ttcagaacgc ttattttaat
     3121 aagatgattg aagctattaa aaatatattt gattatatta tcatcgatac tccacctatt
     3181 ggtttagttg ttgatgccgc aataatcgct aatgcttgcg atggttttat tttagtaacc
     3241 caagcaggca gaataaaacg taattatgtt gaaaaagcaa aagaacagat ggaacaaagt
     3301 ggttcaaagt tcttaggtat tattcttaat aaagttaatg aatctgttgc tacttacggc
     3361 gattatggaa attacggaaa aagggataga aaaaggaagt aagggactct ggtattgaaa
     3421 gaaaaagaaa a
//
LOCUS       DATRME010000117         3330 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRME010000117 DATRME010000000
VERSION     DATRME010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            133..1341
                     /locus_tag="VBC23_002000"
     CDS             133..1341
                     /locus_tag="VBC23_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6843907.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            1535..2030
                     /locus_tag="VBC23_002001"
                     /pseudo
     CDS             1535..2030
                     /locus_tag="VBC23_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(2005..2260)
                     /gene="mobC"
                     /locus_tag="VBC23_002002"
                     /pseudo
     CDS             complement(2005..2260)
                     /gene="mobC"
                     /locus_tag="VBC23_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2270..2407)
                     /locus_tag="VBC23_002003"
     CDS             complement(2270..2407)
                     /locus_tag="VBC23_002003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843908.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2540..3330
                     /locus_tag="VBC23_002004"
                     /pseudo
     CDS             2540..3330
                     /locus_tag="VBC23_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
BASE COUNT         1078 a          674 c          543 g         1035 t
ORIGIN      
        1 atgaagagag agtttgctaa ttagcgtgtc tttttagtgt atccacaaga tagttgtttt
       61 catcatttta ttttgtagtc tttacaaacc taaagaaaaa ttctagaaat gataatgaag
      121 gcggtctatc aaatggttac attcgatttt aaacatgttc aaacattgca taccatatct
      181 caactaccta tttcagtcat gtcacgagac aaggaactcg ttcagttata tggtaacgaa
      241 gactatctgt tgccttacta tcagttttta aaacatttag ctatccctta taaccaagat
      301 atcactgttt atgagggtct ttttgaagaa tcattcttaa tttttcctgt ctgtcaatat
      361 cttattgcta ttggaccatt ttatccctat agccctgata tcaataggca agaacagtta
      421 tccagtcgct ttctagaaca attttctcat cgtagtaaaa aagaaatctt atcctatata
      481 aagcttgtcc cttgttttcc tactaccagt atacgtagtc ttcttgtgtc tatcgatgcc
      541 tttttccaaa cacagtttga ggctagttgc caacaagtca tcaatcattt attagaagag
      601 tcagaacaga ttgttgcgga tcctgatatt gttcttcacc taaaacatac taagaaaaac
      661 tcttttcagt tacccactgt tttaaaccat ctcaatcaca ttattgatct cgttaagctg
      721 ggtaacactc aactgttaaa gcaagaaatt aatcgcctcc catcatccag tgttacctca
      781 tcttcaatcc ctgccctaag agctgaaaag aacttaactg ttgtttactt aacaaaatta
      841 ctagaattaa gtttcgagga aaatactgat gtggctaaaa gttatgcgct ggtaaagcac
      901 tatatggctt taaacgaaga ggctcctgat cttattgatg tttcaagaat tcgctgtgca
      961 gctcttattg atttttcaga atccttaacc aataagagca tctctgacaa gcagcaaatg
     1021 tacaatagta ttctccatta tgtggacaac cacctctact ccaaactcaa agtatctgat
     1081 attgccaact acctatatat ctcagattcc cacttacgct cagtttttaa aaaatactct
     1141 gacatttcct tgcaaagtta tattctaaag gcaaaaatta aggagggaca attactactg
     1201 caaagagggg taccgattgg ggaagtagcg aaattattac atttttacga caccacacat
     1261 tttcttaaaa cctttaaaaa atacgtggga atatcttcaa acgaatatct tactaaatat
     1321 cgtgagacct catgccagta acatagcaat ccatgaaggt acggaattat ttcatcgtcc
     1381 ttttgccttc atctgaaacg actaaaaaca gccttctcgt ttaaacggaa gactcttttg
     1441 ttacctttca aaatccactg gcactacttt ttagtctaaa attttcagct aatccagtcc
     1501 atacaaactc tctctttgtg ctataacata ggttatggat aaaattatta aatcaatttc
     1561 tgcctctggt gccttcaggt cttatgtcct cgatagcacc gagactgtta aattggctca
     1621 ggaaaaacat cataccttgt cgtcttcaac agttgctctt ggtcgcaccc tgattgctaa
     1681 tcaaatatta gcggctaatc aaaaaggcga tagcaagatt acggttaaag tcatcggtga
     1741 ttcttccttt ggtcatatta tctctgttgc agataccaaa gggcatgtta aggggtacat
     1801 ccaaaatact ggtgttgaca tcaaaaaacg gccacaggtg aagttttggt tggccctttt
     1861 atggggaatg gtcattttgt gaccatcatc gactatggca caggtaatcc ctatacctca
     1921 acaacgcctc ttattactgg gaaaattggt gaggattttg cttattactt aaccgaatca
     1981 gagcaacccc ccctgctgtt gggcttaacg tgcttctcga tgaaagtgat actgagcctg
     2041 agaaattaga tggctttggt tgatagcacg cgcaatttga ttgatgttat ttccaatccg
     2101 tgttaatcgt gacaaatgac atattgggat taagaagaac tcttctagca tagactgaga
     2161 aattttgaca attgctctta gcaatccgct catttaattg attcaattct gaatctgtta
     2221 gaaagacttt tttgagattg gtacgataac gataaacaat tgcagcctcc taacccatat
     2281 acttttcacg aaactcacga ctgagtggct gtgtttgatt gacttccaca atcaattctt
     2341 gaacgcaggt caataagatt gacacgtgtt catgagtcac ttgatgattc tctcttgcga
     2401 taatcaaaac ttcctgcaca tctcaactaa tctgctcaaa cttttggtat tgccaaaggg
     2461 tggatgtata ctataaaagt agacaaaact ttgtgtgtta caatctgttt taaaagacac
     2521 aaaaacgaaa ggacaaccta tgtcaacatt taaacgctat gatgaagaat tcaaacaatc
     2581 tcttgttaac ctttatcaaa ctggaaaaac tcagtctgaa ctctgtaaag actatagggt
     2641 atccacttct gcgcttgcaa aatggatcaa acagtactct caattcaaac tcgaagataa
     2701 ttctgtactt actgccaagc aaatacaaga gttacaaaaa cgtaatgcgc agcttgaaga
     2761 ggagaacctt atcctaaaaa aagcaagtgc catattcatg caaaacttaa agtaagactc
     2821 ctcgctgtct atcggttacg ctttgaacac gccacaacaa ccttgtgtcg tgttttacgt
     2881 gtcaatcgct ccacttacta taaatttcta aaacataagc cctcaaaaag agaattggat
     2941 aatcaaattt ataaaaaaca aatacttgag atttatacca aagcaaacaa aagacttggt
     3001 gtgaagtcta tcaaagtcat tcttcaaaga gactacgaca caaaaatctc tgaaggaaga
     3061 atttaccatc tgatgaagaa tatggcgctc cctaaaatgg ctaccgttaa gccaaaaaca
     3121 gctcttaaaa agactcaaaa aacgtatcct caaaacttac tcaaccagaa attttatcct
     3181 gacaaaccta atcaagtatg gtctactgac ttcacctata tttctattgg atataagaaa
     3241 tatgtctatc tctgcgcaat acttgatctc tattctagaa aatgtattgc ttggaaattg
     3301 agtcaccgta tggatgcaaa gttagcatgt
//
LOCUS       DATRME010000118         3158 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRME010000118 DATRME010000000
VERSION     DATRME010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(112..645)
                     /locus_tag="VBC23_002005"
     CDS             complement(112..645)
                     /locus_tag="VBC23_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6843909.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
     gene            complement(729..1505)
                     /gene="recX"
                     /locus_tag="VBC23_002006"
     CDS             complement(729..1505)
                     /gene="recX"
                     /locus_tag="VBC23_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO6843910.1"
                     /translation="MKITKIEKKKRLYTLELDNTENLYITEDTIVHFMLSKGMIINAE
                     KLENIKKFAQLSYGKNLGLYYISFKQRTEKEVIKYLQEHDIDSKIIPQIIDNLKSENW
                     INDKNYVQSFIQQNLNNGDKGPYVIKQKLLQKGIKSKIIESELKAINFQDLASKISQK
                     LYKKYQNKLPLKALKDKLMQSLTTKGFDYQIGHTVIQNLEIEKDQELEDDLIYKELDK
                     QYQKLSKKHDQYELKQRIINVLMRKGYQYEDIKSALREYL"
     gene            1604..2959
                     /gene="rlmD"
                     /locus_tag="VBC23_002007"
     CDS             1604..2959
                     /gene="rlmD"
                     /locus_tag="VBC23_002007"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6843911.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
BASE COUNT          988 a          447 c          558 g         1165 t
ORIGIN      
        1 ttaaaaaagt agaaagatca ctttctactt tttttatttt aagtatatat ttttgcaata
       61 gtattgtaat taaatttgat attaataaaa acacactatt tagtgtgtta gttagcgctt
      121 tttcaattca aggtaccgtt tataccagat attgatatat gatgatgaaa aggggccttt
      181 attctcattt atccattcta ccaatatttt tacattttcc tttaatatat aatcaatatc
      241 ggtaggatag ttcatctgag ctttatgctg ttcatattcg tccacatcca gtagtctttt
      301 ttcaccatct gcaaatactt tgacatcaag gtcgtagtcg atgtacttga gtgcttcagg
      361 atccaagata tacggacttg ctagattaca atagtaggag acaccagttt cacgtatcat
      421 agcgataatg ttaaaccaat attttttatg aaagtatact attgcaggct ctcgtgtcac
      481 ccagcgtcga ccatcatttt ctgttactaa agtatgatca ttaacaccaa tgagggcatt
      541 ttcggttgtt tttaatacca tggtgtcacg ccaagttcgg tgtaaactac catcatgttt
      601 gtaactttga attgtaataa agtcgccttc tttaggtaat ctcatagcct accaactttc
      661 tacaatttat acgtctgtta tagtttatca taattttctg taaaatacat cgtttgagag
      721 ttattatctt ataaatattc tcttaaagca cttttgatat cttcgtactg gtatccttta
      781 cgcattaaga cattgattat acgttgcttt aattcatact ggtcatgttt ttttgataat
      841 ttttggtatt gtttatctaa ttctttataa atgaggtcat cttcaagttc ttgatctttt
      901 tcaatttcaa ggttttgaat tacggtatgc cctatctgat aatcaaatcc ttttgtagtt
      961 aagctctgca tcaatttatc ttttaatgct tttagtggaa gtttgttttg atatttttta
     1021 tagagttttt ggcttatttt ggatgctaag tcttgaaagt ttatagcttt aagttcagat
     1081 tcaattattt tacttttgat acctttttgc aataattttt gttttatcac gtatggcccc
     1141 ttatcgccat tatttagatt ttgctgtatg aaagattgca cgtagttttt atcgtttatc
     1201 caattctcac tttttagatt atcaataatt tgtggaataa ttttagaatc aatatcatgt
     1261 tcttgaagat atttaataac ttctttctcg gttctctgct tgaaagaaat ataataaaga
     1321 cctagatttt taccgtatga tagttgggca aattttttaa tattttcaag tttctcggca
     1381 ttaattatca tccctttact taacataaaa tgaacgatag tatcttctgt tatatataaa
     1441 ttttcggtat tgtctagctc tagagtgtaa agtctttttt tcttttcgat tttggttatt
     1501 ttcatagttt ttattatagc aaaaatgata aaatagtaaa aatgagtctt tttttaaatg
     1561 ttatggattt atagtatttt cttagttagg gagtaatgaa ctgatgaatg tggttttaaa
     1621 acagcgaatt cctttaaaaa ttaaacgaat gggaattaat ggtgaaggaa taggttttta
     1681 taaaaaaaca ctaatctttg ttccaggggc tctcaaggga gaagaggtgt tttgtcagat
     1741 ttcttctgtt agacgtaatt ttgcagaagc taaactgtta aagataaata aaaagtctaa
     1801 aaatagggta gatcccgctt gttctattta taaagaatgt gggggatgcc aaataatgca
     1861 ccttcaatat gataagcagc tagagtttaa aacggatatt attcgacagg ctctaatgaa
     1921 atttaaacca gagggatatg agaattatga aatccgtaaa acgataggta tgtcggaacc
     1981 tgaacactat cgtgctaaat tacaatttca agtgcgttcg tttggaggga atgtaaaggc
     2041 aggtttatat gcacaaggga cacatcgatt gattgatatc aaagattgtt tagtgcaaga
     2101 taggcttact caggaaatga taaatcgagt tgcagaactt ttaggaaaat ataaattgcc
     2161 tatttataat gaacgtaaaa tagctggtgt tcgtactgtt atgattcgac gagcacaagc
     2221 gagtggagag gttcaactga tttttatcac aagtaaaaga cttgattttg atgatgttgt
     2281 tattgaatta gttcgagagt ttcctgagtt aaagacagtt gctgtaaata tcaacgctag
     2341 taaaacaagt gatatctatg gtcaaataac agaagttatt tggggtcaag aatccatcaa
     2401 tgaagaagta ttagattatg gtttttcgtt atcaccacgt gctttttatc aacttaatcc
     2461 taaacaaaca caaattttat atagtgaggc agtcaaggcc ttagatgtga aggaagatga
     2521 tgatttaatt gacgcttatt gtggagtagg aacgattgga cttgcttttg ctggaaaagt
     2581 taaaagtgtt cgtggaatgg atattatccc tgaggctatt caggatgcta aagagaacgc
     2641 acttcatatg ggttttacta atacacatta cgaagctgga aaggcagagg atattatccc
     2701 taggtggtat agtgagggat ttcgcgctaa tgcgttaatc gtggatcctc ctcgcacagg
     2761 cttagatgat aaattgctaa atactatact aaaaatgcct ccagaaaaaa tggtttacgt
     2821 ttcttgtaat acatctacgc ttgctagaga cttagtaaca ttaacaaaag tataccatgt
     2881 tcactacatc caatctgttg acatgtttcc acatacagca aggaccgagg ctgtagtgaa
     2941 actacaacgt aaggtataat aaagttttct aatctaaaaa taagagtatt tttgggtatt
     3001 cttattttta gatagtaatg gtgagatatt aatatagcat agtataaaac acatggttta
     3061 agctatttga tacggaaatt taccttagaa atggtaaata attccattaa gtatattcat
     3121 ctttgcaaat ttgaccttgc cttttaagtt tcattggg
//
LOCUS       DATRME010000119         2917 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRME010000119 DATRME010000000
VERSION     DATRME010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..343
                     /locus_tag="VBC23_002008"
     CDS             <1..343
                     /locus_tag="VBC23_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843912.1"
                     /translation="SEVKKDKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWS
                     SVKVEQSGNGTPQGGDYNLSLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPY
                     IHKNGIWHIYE"
     gene            407..2041
                     /locus_tag="VBC23_002009"
     CDS             407..2041
                     /locus_tag="VBC23_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6843913.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2060..2332
                     /locus_tag="VBC23_002010"
     CDS             2060..2332
                     /locus_tag="VBC23_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843914.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2342..2692
                     /locus_tag="VBC23_002011"
     CDS             2342..2692
                     /locus_tag="VBC23_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843915.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELRRVYNDRVD
                     ELYQKAKQNEAKKG"
BASE COUNT         1106 a          494 c          536 g          781 t
ORIGIN      
        1 atcagaagtg aagaaagata aaaaaatgac aaaaaaagaa caattagcct atctcaaaga
       61 gcatgagcaa gaaatcatag attatgtaaa attacataac aaccaaattg agtccgttca
      121 attcgattgg tcaagtgtaa aagtagaaca aagcgggaat ggaactccac aagggggtga
      181 ttataatctt tcactgagag gaaagtttaa tcatctacaa aattcaaaat taatagttga
      241 tttttattta gctcataaaa atgatatccc aaatatcaaa tcaatgggaa tgctaaataa
      301 gccatatata cataaaaatg gtatttggca catttatgaa tagcttgaat catcctatat
      361 aaagtaatag aatttttacc acaagcaagg tagaggagaa gaataattga gtaattggaa
      421 cttagataat tttgataact tacatagctc tttagcagaa agtgcatata atagtcgacc
      481 taattcattt cctgaattat ttgagactga ctcagtaaca gaagtaaaat tttctcaacc
      541 ctcagaagat aacaaaggtc aaatcacaca gggtggcact aacctcccca atgatggtat
      601 tgtctacctg caaccagata agtccttaaa atctattgat gaaaatgtaa aggtcctcat
      661 tccagatgtc aatggaggct atcacactga gcattatgtt actcattctt atcaaaaagg
      721 tgtgcttact gacgataagg caggttttaa tgcttactac ctgtcagata ctgaaaaaat
      781 agatagcact acaaagcaca cttatctagc cattcgtggt agtgatggga ttggtctaga
      841 tacactaaac gattgggtta gtaacaatgc aatgtttgct gtctctaaca agtatattcc
      901 acaagcgaaa ttggctaata aggcaatgaa agaaaaaatt gccgagctaa aaggaaaagc
      961 acctggagct gttatggata ttactggtca ctcactcggt acaattgtat catctcaagc
     1021 agtagttaat cttagctatg cagaattgga gaatgttggt caagtagtcc ttttcgatgg
     1081 ccctgatgta tcaagaagct tggaaaaaat ggaaggcatt tctgctaaaa aaatccaaga
     1141 agctgggaag catgttacct actatgtcaa tccctttgat atagttagta tgcttaatcg
     1201 tgaaaagcca tggaaagacc aattcgggaa agtaaatgtc attgttccta gtaaatatac
     1261 gggtactttt gatgagcaat cttctcacga ttttggtgcc ttccaaattg atagtagcgg
     1321 aaatccttta gttgcttctg aaagctatca tccagaatta ttaacatctg gtcaagactt
     1381 agctagactc gaaaaaaaat tcataaagaa tcttttagga gagcattatg attcggataa
     1441 gctaggacca gtactcaaag gcgctctgac tggggacctg actcgactaa ttgcaacatt
     1501 aaaagcttgt ggtatcagtg tcactgtaaa cgaagctaaa aaactgtacg ataacttcca
     1561 aaaagactac aaagccataa tttccaaagc taaaaaagat ggcctcgagt gggatcgcaa
     1621 aaacatcgca agcttccata gtaggatcaa atcagcatca ggcagtcaac ggattttact
     1681 tagaactcaa atgctctata tggcaacgca attagcagaa gcagatgttt cagaaaaagt
     1741 ctctgcaaca aaaaaacata ttagtgaagc taaggatacg attgtagaaa tatcgacatc
     1801 cactatttca tcagctgaaa taatggctat gcaccttggc caatcagaag ttgatgctct
     1861 ggtttcagat ataaaaatgt caacagtttg gaatgatagc gtagagactt ctgattatga
     1921 agcacttgat cactataaaa caaaaatgac cacatttacg accaatttag taacagttgc
     1981 acaaaattta acagctcaag atgagcaact agctggtgat attgtcacaa acttatccta
     2041 aaatagagag attaaatgaa tgtccaaaga attgaaattg cccttgaata aaatgttgaa
     2101 agaacaagaa agaatagcta ttaaggcaat agttgctaca gcaaaaagca ctattacgga
     2161 tactggaaca aatttaaaag caaccataga gacagatgcc caagacctaa aaaactatgc
     2221 atccacctat aacgccaata tcacggatgg tcttgaaggc cagtcagctc aagcagcatc
     2281 tgatttcttg acacaacttc caaaaccaga gttagataat ccagtcaact aggaggctag
     2341 tatggtagat acaaaaaaac tgcaagaact cgatcaagag tatgatcaaa atctcagaaa
     2401 catttatcgt aacagagagc aactagagga tgactttcac ttgtttatgg caagaacaga
     2461 tagcctaaaa gagtctgtct atcaagcaac tttaggtcaa ggttgggaat taccccaaga
     2521 agctcatgca cacctatata atatggatga taataaggat acattcatat cagaattcaa
     2581 tgaatatatg gaaaagcttg aagaaaagga aatagagctt cgaagagttt ataatgatag
     2641 agtagatgaa ttgtatcaga aggctaagca aaacgaagca aaaaaaggat aaaaattttt
     2701 tcaagtaata tatataaata tgaaaaataa agaatcttgc agtaaaatga gtatttattt
     2761 ttcacgacga acagtcacta caaaatcact aaaatctcaa aattacttgg tttttaagtt
     2821 aatgtctaat actcaaacct tgttaaatca atgtttgtta tctttaaaaa gtgctcagta
     2881 gcactcaaaa tccagtgtcc tcacggacgt gtcggtt
//
LOCUS       DATRME010000120         2863 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRME010000120 DATRME010000000
VERSION     DATRME010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            291..1148
                     /locus_tag="VBC23_002012"
     CDS             291..1148
                     /locus_tag="VBC23_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEO6843916.1"
                     /translation="MKNISPLRYPGGKSQVYDFVKELVNLNETNTYIEPYMGGMGIAL
                     KLLVNNDVKQIMVNDYDRSIYAFWYSVLNHTNQLIDKIESTPITIEEWKKQRKIQQGK
                     ETCHDLLTLGFSTLFLNRTNRSGIIKAGVIGGLKQDGNYKLDCRFNKEKTIKKIRLIA
                     SYKKQIKLYNIDAEKFIRLNITKTKKSFTFFDPPYYSKGPGLYTNFYNHENHLSLSNT
                     IKNYMSNKNWILTYDLSEEIFQMYKEFKYEKYYLNYSVTKPSKGIEYIFYSNGLTVPE
                     NTINIKKAN"
     gene            1268..2410
                     /locus_tag="VBC23_002013"
     CDS             1268..2410
                     /locus_tag="VBC23_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6843917.1"
                     /translation="MWSEKHKSGKVNFIERYKDPYTSRWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTDLLDQWWGFYQQEIKRSSIASLRGNIRDIRESFGID
                     IKVVNIDPKYVQTYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPRI
                     KKTLEDWKKIEEKYLEEEEIKRLLKELFRRPSTYRLGLLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYMNGEKTSPKTLASYRETIMTKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTKNGIPVQINSFNLALKKANKRLKHPINKNITSHIFRHTLVS
                     RLAENNVSLKAIMDRVGHADAKTTVQIYTHITKKMKSNVADIMENY"
     gene            complement(2499..2774)
                     /locus_tag="VBC23_002014"
     CDS             complement(2499..2774)
                     /locus_tag="VBC23_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6843918.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT         1128 a          456 c          452 g          827 t
ORIGIN      
        1 aaatcagatt ttgcattaaa atttcctata cataaaggta tgatatcaga aaacggtcca
       61 ttttctggag atttcgacga atatactaat gaatcaaaaa aaataaaaga atggtttaca
      121 aattataggt ggatttgtga tgaagcaatt tattactact tttcagacaa tgaagaagtt
      181 attttacctt tcgcttcaaa aattgaaaaa ttttacagaa aatcaataat ataataattc
      241 tggccataat taattaaatg ttatatcata agtgaaagga ggtaagccga ttgaaaaata
      301 taagcccgtt gagataccct ggaggtaagt ctcaagttta tgattttgtt aaagaacttg
      361 ttaaccttaa tgaaacaaat acatatatag aaccgtatat gggaggtatg ggaattgcat
      421 taaagctact tgttaataat gatgtaaaac aaatcatggt aaatgactat gatcgttcaa
      481 tctatgcttt ctggtactct gtcttaaatc atacaaatca gttaattgat aaaatagagt
      541 ccacacctat cacaattgaa gaatggaaaa aacaaagaaa aattcaacag ggtaaagaga
      601 catgtcatga tttattaact cttggatttt caactctatt tcttaatcga acaaatcgtt
      661 ctggaataat aaaagcaggt gtcatcggtg gactaaaaca agatggaaat tataaattag
      721 attgtcgttt caacaaagaa aaaactataa aaaaaataag attgattgca tcttataaaa
      781 aacaaattaa actttataat atagatgctg aaaagttcat tcgtttaaat atcaccaaaa
      841 caaaaaaatc tttcactttt tttgaccctc catactactc aaaaggacct ggattatata
      901 caaactttta caatcatgaa aatcatctaa gtttatccaa cacaattaag aattatatgt
      961 caaataaaaa ttggattctc acgtatgatt tatctgaaga aatttttcaa atgtataaag
     1021 aatttaagta tgaaaaatat tacctgaact attcagtaac aaaaccaagt aaaggaatag
     1081 agtatatttt ctactctaat ggattaacag tcccagaaaa tacaataaat ataaaaaaag
     1141 caaattaaaa aaagacctac acagcgccgg caagcaaacg tgtaggtcaa gttggtaata
     1201 gtaaaaacct gctttaagta ggctctttac tgtacccatt ttaacaaaaa aaatgaggta
     1261 aaaaacaatg tggtcagaaa aacataaaag cggaaaagtg aattttatcg aaagatataa
     1321 agatccgtat acaagcagat ggaaacgtac atcagtactg atggaaaaag atactcctcg
     1381 aattagaaaa gaagctcaaa gaatattaga tgctaaaata gctgacatcg taagaaagtt
     1441 acaaacctcc gacatgctat ttacagacct tcttgaccaa tggtggggat tttaccaaca
     1501 agagataaaa cgctcctcta tagcctctct gagaggcaat ataagagata ttagagaaag
     1561 ctttggaatt gatattaagg ttgtcaatat cgaccctaaa tatgtccaaa cctacttgga
     1621 taacttagat tgctccagga acaaaaaaga gcgaaacaag tccatgctta atcttatctt
     1681 tgattatgct gttagtctag atattatcaa agataatcct gcaaggcgtg ctaaacttcc
     1741 gcggataaag aagactttgg aagattggaa aaagattgaa gaaaaatatc ttgaagaaga
     1801 agaaatcaaa cgactattaa aagaattatt tagaagacct agcacttacc gtctgggatt
     1861 actatcagaa tttatgagtc ttaacggatg ccgtatcggt gaggctatta gcatagagcc
     1921 ggataatatt gattttaaaa acaaaacttt acagttgcat ggtacttatg atcgtaccaa
     1981 tggttacatg aatggagaaa agacctctcc aaaaacgctt gcgtcctatc gcgaaaccat
     2041 tatgactaag cgagaaatgg aaattttaca agagatggaa tttattaatg agctcgaaaa
     2101 aaatacaaat aaacgatata gagacatggg atatatcttt acaaccaaga atggtattcc
     2161 tgtacaaata aattccttta atttagcatt gaaaaaagca aacaaaagac ttaagcatcc
     2221 aatcaataaa aatatcacta gtcacatctt ccgccacaca ttagtcagtc gtcttgctga
     2281 aaataacgta tctttaaagg caatcatgga tcgtgtagga cacgctgacg caaaaactac
     2341 agtacaaatt tatacacaca taaccaagaa aatgaaatca aatgtagctg atatcatgga
     2401 aaactactaa tttcatgccc caaatatgcc ccaaaaataa aaaaaagaac cttgtcgcta
     2461 tcaataaaca ttgatatgac aaggtttttc tagacgaatt atttaacagc gtctttaaga
     2521 gctttaccag ctttaaatgc tggaactttt gatgctgcaa tttcgatttc tgcaccagtt
     2581 tgtgggttac gacctttacg agctgcacgt tcacgaactt caaagttacc aaaaccgatt
     2641 aattgtactt tttcaccttc agcaaggtaa tctgctacag ctgcaaaaac tgcatctact
     2701 gctgctgctg aatctttttt agttaattct gtagcttctg ctacttttgc aattaaatct
     2761 tgtttgttag ccatgttaac aaatcctcca aataaattct aaacttatac aacataagtt
     2821 ttaacagatt atatgatatc taaaaaaagg cttaaggtca agt
//
LOCUS       DATRME010000121         2825 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRME010000121 DATRME010000000
VERSION     DATRME010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            164..931
                     /gene="cfb"
                     /locus_tag="VBC23_002015"
     CDS             164..931
                     /gene="cfb"
                     /locus_tag="VBC23_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6843919.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1104..1406
                     /locus_tag="VBC23_002016"
     CDS             1104..1406
                     /locus_tag="VBC23_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6843920.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1563..2426)
                     /locus_tag="VBC23_002017"
     CDS             complement(1563..2426)
                     /locus_tag="VBC23_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6843921.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2731..>2825)
                     /locus_tag="VBC23_002018"
     CDS             complement(2731..>2825)
                     /locus_tag="VBC23_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6843922.1"
                     /translation="EDRENVLFSDDIAEIEDFITNYKVPSTRQY"
BASE COUNT         1029 a          400 c          523 g          873 t
ORIGIN      
        1 ttagttttat gaaaaaaatg cagtttattt gtaataatgt aaaatatagg ggaaaagaaa
       61 gcgcttgacg accttttgga caagtagtaa gatacaaaca tgggccctgt aaattaaaaa
      121 tactgcagta aaagtgattt tagtttaaag gaggaaattt attatgaacg ttaaacatat
      181 gatgtatcta tctggaactc tagtggctgg tgcattgtta ttttcaccag ctgtattaga
      241 agtacatgct gatcaagtga caactccaca agtggtaaat catgtaaata gtaataatca
      301 agcccagcaa atggctcaaa agcttgatca agatagcatt cagttgagaa atatcaaaga
      361 taatgttcag ggaacagatt atgaaaaacc ggttaatgag gctattacta gcgttgaaaa
      421 attaaagact tcattgcgtg ccaaccctga gacagtttat gatttgaatt ctattggtag
      481 tcgtgtagaa gccttaacag atgtgattga agcaatcact ttttcaactc aacatttaac
      541 aaataaggtt agtcaagcaa atattgatat gggatttggg ataactaagc tagttattcg
      601 cattttagat ccatttgctt cagttgattc aattaaagct caagttaacg atgtaaaggc
      661 attagaacaa aaagttttaa cttatcctga tttaaaacca actgatagag ctaccatcta
      721 tacaaaatca aaacttgata aggaaatctg gaatacacgc tttactagag ataaaaaagt
      781 acttaacgtc aaagaattta aagtttacaa tactttaaat aaagcaatca cacatgctgt
      841 tggagttcag ttgaatccaa atgttacggt acaacaagtt gatcaagaga ttgtaacatt
      901 acaagcagca cttcaaacag cattaaaata atatttgtat ttttcgtgtg atgctgtcga
      961 cttcgtgatt ttgtactacc atgattgtta tgattaaaag atttacgaca atagtcataa
     1021 tagtagaacg atgtcaccat tttaaataat aaagtgatta gtcatttgac taaatttgcc
     1081 aagtatcaaa ggaaataaag attatgacta aaaagataac tgttgtagca ttagaaacat
     1141 tgattgccca gcataataat atccatttga tagacgttcg tgaagagcat gagtatcgtg
     1201 gagggcatat tccaggtgcg ataaatcttc ctttgagtca actcagtcat aagtttgaac
     1261 agttagataa aaataaggaa tattatcttg tttgtcaacg agggggaaga tctattagag
     1321 catgccaatt tctcgagttg aaaggctaca aagtaatcaa tgtagatggt ggcacagaag
     1381 cgtggagagg aaatctagaa gtgtaaatat gtcctgtgta gtctaccata tttgataata
     1441 aaaaaattga cattatctca ctattaatga ccttttagta aacataaaga aaagtatgtg
     1501 ttttgaaaag gaggatgatt atccttttca aaaccatact ttttgtgtct tgtggcttgg
     1561 tcttaattaa tagttgttag gacaatgtcg taaatgacaa ggagaataaa aggccaacta
     1621 gtaagtaata attttgtgtg ttgtttagag tttattgagt cagcctctcg aacgttagag
     1681 tgcagactta tacgacggcg agcagtattc ctatgcgtaa aaaggtaata taatataaat
     1741 aagattgcta aaattagcaa tatattttca aaataagtcc cgtataactc gtttactgtt
     1801 gatagctggg tgaaaatttc tacgataaag ttattgcaga tatgaaggag tagggctacc
     1861 ttaaaggaat aatggtaagc gataaaaccc attatgatac ccgcaataga tgtagtcaat
     1921 agctgagaaa tgtcttggtg cattaaacca aataggatgc cagaagtgat aatggcaaac
     1981 catttaccat acttggataa taaattcaat actattcctc ggtataaaac ttcttcaaaa
     2041 aaaggaccta caacaacaga ataactaatc ataagtatac tgaatgtggc atcataattt
     2101 tgactttccg attccataat attgagatga tttagcacag catcccatat tgtggagact
     2161 atattattag tagtatatat aatgcttaac aagaataaaa tagtggtgaa agtgattgta
     2221 tgatgagttg ccgttagatg tttttttaaa tttttaaaac ccataaaaaa acaaggaatg
     2281 agtaaggcga ttaaagtact tgctatacct atccagacat cattttgtaa gatttcagca
     2341 aatagtgtag tcgccaaact taaaacaata gcctgagcta aaagtatcag cccgattagg
     2401 ttaagtctct ttttttcttg attcaattga ttacctcctt attaataacg gtaacgacaa
     2461 agaaatatta taatgtataa tgtataggtt tctaagtcat tagtcaatag ggacgagcac
     2521 ttggatactt ctaataagat aaaatacata gacaagacta tggttttggc ttctctaaag
     2581 attacctaaa aatttataag ttctcgagca gaaaggatga gtttggatat tgagaggatg
     2641 ggcttgacca gctgaaagaa aaatgaacca agggtaatcg cttcgaaaaa agaaggaaat
     2701 ttcaattaga aacttccttt ttttatcaat ctagtattgg cgcgtgctag ggactttata
     2761 attcgttata aaatcttcga tttcagcaat atcatctgag aataggacat tttctctatc
     2821 ttcca
//
LOCUS       DATRME010000122         2540 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRME010000122 DATRME010000000
VERSION     DATRME010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            117..962
                     /locus_tag="VBC23_002019"
     CDS             117..962
                     /locus_tag="VBC23_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO6843923.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(1005..2252)
                     /locus_tag="VBC23_002020"
     CDS             complement(1005..2252)
                     /locus_tag="VBC23_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843924.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT          856 a          410 c          512 g          762 t
ORIGIN      
        1 gaaattatta ttaaattaat tatggagttt atatagtaca acatattctt tttcagaaaa
       61 aacagctata ttttgctata ctttaatcaa atatcaatat tagaaagtga ttgcctatgt
      121 tgtctgttgg tttagtttta gagggtggcg gaatgagagg tctttatact gctggagttt
      181 tagatgcttt tctagatgca ggaataaaaa tagatggtat cgtatctgtc tctgcgggtg
      241 cattgtttgg tgttaatttt gtatctagac aacgagagag ggctttgcga tacaataaaa
      301 agtatttatc ccaccctgaa tatatgagtc taagatcatg gcttcgaaca gggaattttg
      361 ttaataaaga tttcacctat tatgaagttc ctatgaaatt ggatgtattt gacgatgaag
      421 catttaaaaa atcaagtatt gatttttacg tagttgctac agagatgaca tctggtaaac
      481 ctgaatattt taaaattgat agtgtttttg aacaaatgga aattttacgt gctagttcag
      541 cattaccagt agtctcaaag atggttgatt ggcaggggaa aaagtactta gatggtggtt
      601 tatctgatag tattcccgtt gattttgccc gtggtttagg atttgacaag ttgattgttg
      661 tgatgactag gccgctcaat tatcagaaaa agccttcaag tggacgattg tataaaactc
      721 tgtataggaa atatcctaat tttgtaaaga cagcctcgaa tcggtaccaa cagtataata
      781 atagtcttga aaaggtcatg agccttgaaa aaacaggcga tctatttgca attagaccga
      841 gtaagagctt ggttattggc cgcttagaga agaatccgga taaacttgat agtatttatc
      901 agcttggtat gaaatatgct aaaagtgtga tgcctgagct gaatagttat ctaatgaaat
      961 aaagaaaaca gtatttctca attgaggaat actgttttgt aatatcagtc agttggtttg
     1021 acatctaatt gtgttaaatt agtgtgatat ttttgacgtt ttaattgctt aatgcttagc
     1081 attgataaga aactaacgat atagagactt gctaagaaaa gcatgaccat agagacattt
     1141 gaaacgtcta gaatcattcc gatgataaca gacgaaaagc cacctattgc acgaccaaca
     1201 tttaagataa gattgttagc tgttgaacga atatgatgtg gatagagacg tgtaatcatg
     1261 gcaccatagc ctgcaaacat accattaaca aagaagccga caactgcccc gcctataatc
     1321 atagaaggca ttgttgttgc aaattgaaag agatagacac aaattgcaga tgatagaagg
     1381 aaacacccgt atactaaacg cggtccaaat ttatctaaca attgaccaaa tactagcatg
     1441 ccaagacaca ttccaaaaat ggttgcaatc atccataatg atgaattctt gacagaaacg
     1501 tttaagttgg tttggataat agttggtaac cagttcatca tgccaaagta tccggcaatc
     1561 tgaacagttg tcataaccat tagtgctagg ctctgtgtgg ctaatacagg agtatcaaat
     1621 aaagctttta tactgatatc tagaggttca tcatctgcgt ctgtattata ttgatctaag
     1681 atatctttat catcaatagc aaattgcatc cataggacga gaactatagg aagaaggcca
     1741 aagagaaaaa gtccccgcca accgagtgct ggtgctagcc atcctgctaa gagtgctgag
     1801 ctaatagatc cgacttgacc agcgatacca tttagtgaag aaatccggcc cattttatta
     1861 gctgggacaa ttcctgccat aatggcaata gcgataccat attcgccacc aacgccaatt
     1921 cctgcaatga agcgcatgat atagagatat gataaattag tagtgaagaa aactaaacca
     1981 gttgcgattg aaaagattag aatcgtccat ttgaaaactt tgaatttatt gtagcgatca
     2041 gctaataaac caaagattag tcctcccacg agcattccca agttggtaat tgtcccaatc
     2101 caaccagcct gtgccgcaga taaatgaaga tcagtaatga tagttgacat agaaaatgca
     2161 aggaacatga tatttaaatc atcggttccg gatgcgacaa tggcagcgat tagggcgcgc
     2221 caattattct tattgatatt taatggtgac ataagtctcc tcctttaatg atggctcaca
     2281 gaccaccgtt attaaatact gtcaatagaa aaaattctaa agcagagatg tcatagctgc
     2341 ttcaactcat tataatgtaa aagaaaaaac tcaagctgct acccttgagt tctataaacg
     2401 ctcgcaaaaa tagtaggatt tctgtataga atcctatttt ggtcgcttat atgtctcaca
     2461 ggacacggtt aaagcattat gtcatttgga aatagtatag agtaagttta gtttattgtc
     2521 aaggataagt ggggagagaa
//
LOCUS       DATRME010000123         2458 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRME010000123 DATRME010000000
VERSION     DATRME010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            33..2192
                     /locus_tag="VBC23_002021"
     CDS             33..2192
                     /locus_tag="VBC23_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843925.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            2332..>2458
                     /locus_tag="VBC23_002022"
     CDS             2332..>2458
                     /locus_tag="VBC23_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6843926.1"
                     /translation="MALFFTFLKVSQVIFLVLLIAYASFLVISLVIGGVHLYEKNE"
BASE COUNT          860 a          378 c          461 g          759 t
ORIGIN      
        1 aaaatactaa tactttactg gagtagaaca ttatgaaaaa atatctaagt attgtagccc
       61 ttttaactat tttgtttagc tctatttatt atttgtatta ctttgatggt agtttgtatt
      121 taccaaaggg cttattaaaa gaaaatacaa gaactaactt tgttgttaaa ggtgatactg
      181 tacttcacaa gcccaccaat aaaccttttg ttgttaaagg agtagacgtt gagtcttcct
      241 tagcgggtta tcatcacaac gattttccta ttactcaaaa aacgtatcgt gaatggttcc
      301 atttaatttc caacatgggg gcaaatactg taagagtcaa ggtaccgatg aatgttgcat
      361 tttacgatgc cttatatcac cacaacaaag aatcaaagag gccactgtat ttgttgcaag
      421 gaatacgtat agattcttat cgcaataatg cttctataac agcttttaat gataattata
      481 gggggtattt aaaacgagaa gcaaaaggcg ttgtggatat tctccatggg cgtaagcaag
      541 tatggaatac tgattttggt agccgtcatt atcattatga tcttagtcct tgggtacttg
      601 gttatgtcgt aggggatgat tggaatagtg gtactgtcgc ttatactaat catcaagaga
      661 aaaaaacgca atataaagga cgttatttta aaacttctgt ggcagctaat ccatttgagg
      721 tcatgctagc tcaagtaatg gatgaattga cacattatga gacagctaaa tatggttggc
      781 aacatttgat tagtttttca aactcaccaa caacagaccc ttttcattat cgaaaaccat
      841 ttgaggcaca ggctcctaaa tacgtacaac taaatgtaga aaatattcaa gctaattcaa
      901 atgttaaagc aggtatgttt gcagcatata aagctattga tttccatcct cgatacaagg
      961 attatctatt atttgataaa gagaatatca gtaaagaaga tagacaaaag attaaagaac
     1021 tttctttgtc acagggatac gttaaactgc taaatgctta tcacaaaatc cctgttctag
     1081 tcacgggtta tggctattcg acagcgagag gtattgccca aaaagaaatt gataaacgtc
     1141 ctctgccgat taatgaaaaa gaacaaggtc agcgtttact agaagattat gaatctttta
     1201 tatcatccgg tagttttgga gcgactatca atgcatggca agacgattgg aatgcaaggg
     1261 cgtggaatac atctttcgcc acaaataaac atagtcaatt cctatggggg gatgcacaag
     1321 tatttaatca aggttatggt ttattaggct ttaaaaacgc aaaacatcat tatcaagttg
     1381 atggtaaaag aggcaaagga gagtggaaac atcctctgat gactagtgca acaggagatg
     1441 acttatatgc tagcagtgat gaaagctatc tctaccttgc gattaaaaca aaacctgaaa
     1501 aactaaaaga aaaacgatta ttaccaatag atattacacc aaaatctggt agtagaaaaa
     1561 tgaatggtag taaggtcaca ttttctaaat ctagtgactt tgtattgtct attgatccaa
     1621 atggcaagtc tgaattattt gtccaagagc gctataatgc cttaaaagcg aactatcttc
     1681 gacagcttaa cggtaaagat ttttatgctt tcccaccaaa gaagaacagt agtaattttg
     1741 agcagataaa tatggtattg agaaatacaa agattgttga agacatggaa aaagtaaaag
     1801 caacagagag gttcttacca actcatccta ctggtcttct caaaacagga acaactgata
     1861 ggcaccaaaa aacatttgat tcacaaacag atatttcgtt tggaaaggac tttatagagg
     1921 tcagaattcc gtggcagttg ttgaattttt ctgatccatc atctcaaaaa attcacgatg
     1981 attactttaa acattatggt gtgaaggagt tagaaattga gagcattgct ttaggattag
     2041 gtgctaatag caaagaaaac acactgataa agatggcaga ttatcgtttg aaaaattggg
     2101 agagacccga taccaaaacc tttttaaaag actcctatta tagtattaag aaagaatggt
     2161 ctaaagaaag agagagaaca tatggtacat aatattttat atttttacat tttgatttgc
     2221 ttagcaatta ttgtttttaa cattcactat atcttaagac aacgattaga taagcttagt
     2281 tggcttcgta ttcggaaaaa ggatataaat atcaaataaa ggggattttc tatggcatta
     2341 ttttttacct tcctaaaggt atctcaagtc attttcttag tattattgat tgcctatgcc
     2401 agctttttag tgatttcatt agtcattgga ggtgttcatc tctatgaaaa aaatgagc
//
LOCUS       DATRME010000124         2453 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRME010000124 DATRME010000000
VERSION     DATRME010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            288..1571
                     /locus_tag="VBC23_002023"
     CDS             288..1571
                     /locus_tag="VBC23_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6843927.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
     gene            1726..1965
                     /locus_tag="VBC23_002024"
     CDS             1726..1965
                     /locus_tag="VBC23_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843928.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            2065..>2453
                     /locus_tag="VBC23_002025"
     CDS             2065..>2453
                     /locus_tag="VBC23_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6843929.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILG"
BASE COUNT          878 a          416 c          366 g          793 t
ORIGIN      
        1 aaaggttttc atttgtatat ttaaatattt tttcgaatta ttatattatt tgtcgctttt
       61 ttatggtaat ataaaataaa tatagatttt aaattaatat atacatttct atatttattg
      121 atttttatat cagtataact tagtgtcgta gagattaatt cctatcattc actgctttat
      181 tttaggataa ctaaacaatt tattgtttaa aatgaaagga tttatgaatg tctaggaaaa
      241 catttaaacg tatactatcg attggagttt gcacgctcgt actatcgatg agtctttatt
      301 acactgaaaa agcccacgct attgctggtc ctagtgaccg ccaatacgta gaaaacccaa
      361 atcctcacat tattgtaaat gttacaggta ctgatcaaaa cggaaatagc attttaccgc
      421 attatatcga agtcaatgta aagatgggac aaactttaag taaagaagaa attctagatt
      481 atattgctcg aaatttaaac tctagtgttg gaggagaaag taaaaacgtt caatacagca
      541 acatcgagtt taaggaaagt gcttatctga agcgtcaatt agatgatggc aagacagaag
      601 aaatagcaat tgataacgac ggtgttactg tacctaaaga cggtccaaac aaattttgga
      661 ttgacgttcc agtaacttgt actgttactc ctatcgtaac agaaacacat gaagttcgat
      721 gggggactcc agtcgctata tcacaccgta tttactttgt tgaagaatct tctggaaaag
      781 ttttagatga atacacaaat ctacacactg ctgattcgga acttaacggt tatcgtgttg
      841 gagattatat cacagactat gcactttcta agtctgctta cgaagctttt ttaaactctc
      901 gtttagataa agaaggttac aaacttcaac atcgtattag cacgaatgta cgacaaaacc
      961 ttcaaattaa taaattgatt ttcaattatg actttaatga ggaaaatatt tactaccaaa
     1021 tcgggaatat ccgtccacta ttaagtcgct catcagctga agtagaatct gacatcatta
     1081 cagaacgcta ctatgtttct aaaaatgcta aaagtttagc acgtacagaa tcaaccattt
     1141 cgattaaaat ggttgatgcc aaaactgaac aaccgctatt taaccacaca ttaactggtt
     1201 atcaattggt aactgtctcc catgtctata acagactctt tgaagaaaat cttatcccaa
     1261 ctacaaaatc aggagaaaga tattttattc aaaatatgaa aaaaacagct gaacaagaat
     1321 atactgttta tctttcagaa acaccttatt ctaaagagaa cgctcccgta atttcttatg
     1381 atgcaagacc tgttgattgg gattatcact caggcgcttc aggatcactt gaaaatcagc
     1441 ctaacatcta tactgaagaa gattcaactg aatttttggg taataaacca caagcagctt
     1501 gttatccaaa caaacaattt gcttgcgaaa atactgactc taaatacaac tatagctatt
     1561 tagaaaaata gttataaccg atagggttaa tctctcatta tcttatactg attaaaatca
     1621 ttaaacttgt ttccattttg actggaaaca agttttttat atttatttgt cacatataat
     1681 tgacactctt cgaaaaatga tatatactta aaatgtcatc tatatatgac acttttctta
     1741 aattctttac taaaggagct aataatcgtg aatcgtctta gagaatacag aattaaagct
     1801 agcatatctc aattagcttt agcaaaatca gttggtgtcg ccagacaaac aataaacctt
     1861 attgaaaata ataaatacaa tccttcgcta gatctttgta taagactagc ggaatgccta
     1921 caaacagacc tcaatactct tttttgggat agtagacatc tataacagat tagactctct
     1981 aaatctatga aaactcggga ggttaggtcg tattattatt tcaatctcaa actttattaa
     2041 atagttcaag gataggagaa tattatgaaa gaaaagaaaa atttggggat aaaattaata
     2101 aaatccctat atggtattaa tggcccattc gatgaataca gagaacaaaa aatcaattac
     2161 cttggaaatc tattatttgc aaccactagt ctctatcaca ttttatcctc tatcatcgcc
     2221 tggttcacgg taagtaaata tcccacagag actgcatgta ctctcctagc tttaacattc
     2281 tttctatatt tttgtatttt tctaattgtt attccctctt atctcaacag tcatgattta
     2341 ataacgccac tagaagtctc aaaaaatgac tatccagtag caataaagaa aaacaaacaa
     2401 aaatttatca aaatcgggat attttatact gtcatccagt ggcttatctt ggg
//
LOCUS       DATRME010000125         2276 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRME010000125 DATRME010000000
VERSION     DATRME010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..370
                     /locus_tag="VBC23_002026"
     CDS             <1..370
                     /locus_tag="VBC23_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843930.1"
                     /translation="LLLLIFSGGFYMKEQQRKEELKRNREYEVSLVKALKNSYSDIKE
                     IQISNPITTTMPGDWRSTVKIIFSDNDEITYRITHNKDDEINRSGSINEKEHLFLSKK
                     YGRTKNHILVEFSTGEKEKR"
     gene            370..957
                     /locus_tag="VBC23_002027"
     CDS             370..957
                     /locus_tag="VBC23_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843931.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            903..1583
                     /locus_tag="VBC23_002028"
     CDS             903..1583
                     /locus_tag="VBC23_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843932.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            1568..1954
                     /locus_tag="VBC23_002029"
     CDS             1568..1954
                     /locus_tag="VBC23_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843933.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            1988..2269
                     /locus_tag="VBC23_002030"
     CDS             1988..2269
                     /locus_tag="VBC23_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843934.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
BASE COUNT          928 a          296 c          424 g          628 t
ORIGIN      
        1 cctattacta cttattttta gcggaggatt ttatatgaaa gaacaacaaa gaaaagaaga
       61 actaaaacgg aatcgagaat atgaggttag tctagtcaaa gcattgaaaa attcatattc
      121 agatattaag gagattcaaa tttccaatcc aataacaaca acaatgccag gtgattggcg
      181 tagtactgtg aaaattattt tttcagataa tgatgaaatc acttaccgta taacccataa
      241 taaagatgat gaaattaatc gcagtggatc aataaatgaa aaagaacatt tatttttaag
      301 taaaaaatat ggaagaacaa aaaatcatat tttagttgag ttttctacag gagaaaagga
      361 aaaaagataa tgacatattc ttatagcgag tatgatttaa atagattgaa tcaaggtgag
      421 aacgtatata gtgtaaatcc aaactatgtg aaacaaaatg gcggaaaaat aataacagct
      481 aatccaaaaa aatctaatga aacaaattta atagtaacaa agacgggtaa agcgtttaag
      541 gtattaaaaa cttctcctga cgatatgtct ggctatcagg gtatggcagt tgcaccaatt
      601 atcaagggta aagtagatta taacagtgtt gcagttattt ccgccgcaac ggattcaagt
      661 aattataaag atttaattgg cgcagtttca tctgctcaac cacatcaaag ttcgactcag
      721 ttaaaatcag ctgataaatt tttaaaagac gtacagtcac atgacaaatg gactgtgact
      781 cagctttcag gctattctca atctgcttat atgctaaaat taggtgctaa atatcatatc
      841 ccgactacag tatttaatgg atggtttagg tatagcactt taaatgaaga tgaaaaaaat
      901 tcatggctaa gcatcctgaa tattttgcta attttcgaca taaagaggat aatgtaactt
      961 ggtggaacga ttttaataaa ctagatgata aaggttatgg taccgtgaaa tgggtaaatg
     1021 gtaaaagtca taaaatagag agttggaaat ttactgatga tggtcagtta aaagatgaaa
     1081 aaggtaacat cgtaaatcct aagtcaccag ctgttcagtc tgttctttat gaagaagttc
     1141 attttcaaaa agctaaagct aaattaaaaa aatctggagg caaacttagt catagtgaaa
     1201 aagtatattt agattctgaa caggctatat ttattgctaa cggtttaacc acggcatctc
     1261 aaacagcttc tgatgatatc aagaagaatg ctgaattagc taaagaaaaa gcatcagaac
     1321 tatttgcaaa aactaaagtg atgcctcctg gtataacaga tttaagtcca gaagaattag
     1381 ctgatgcata tagtgcgggt ggagttagag aggacaccat agtaacacca attgaaactt
     1441 tctttgatga aaaagttact aatgcacaag agatcacaac ttcatatact aatcttcaaa
     1501 aacaaattga atctggtgtc caaaaattgc tagaagagga ctccaaatta gcaggagaat
     1561 ttaaggaatg gtcacaatac tagatcaaaa tgaaaacaaa attaatagtc taaaaataaa
     1621 cctagggaaa ttggaagata attatcatcg cgaaaatatg gagatagaag ataaattata
     1681 tagccttgag gataaaaaga acgagttaga gacttattta caagaagtct atgaagagac
     1741 aagtcacttt ttgagacaaa atgattcaga tggttcgact tttatgagtt taaatcgaat
     1801 aattgaaagt tatcaaatgg aattaattga tgaatatgaa aactataaac agactatcat
     1861 tgtccaagag gaaaatagtc ggacacagtt tctaaaagac cgagtaaaat tagaaaatga
     1921 catttctagt ttagaaggaa gaaaatacta ctaatttaga tggactataa agtgaaagag
     1981 gcaataaatg agtttaatag gcgctgtatt gaaaggaacc aaagaagaca aagaattcaa
     2041 aaagcaaatt caaaaagctt tagtaaggtt tgtccatgcc ccggaaatag tagaagaagc
     2101 tgattctatt aagacgactt atagtgaaaa gattgatgat atttctgaga ctgctaaatc
     2161 atcaataaaa acgataaaaa accaaattga cactggactt gtaggacaat tttgggaaaa
     2221 agtaaaggca caggtagatt taaataaagg tagttatgat gatttataat cgagtg
//
LOCUS       DATRME010000126         2239 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRME010000126 DATRME010000000
VERSION     DATRME010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1147
                     /locus_tag="VBC23_002031"
     CDS             <1..1147
                     /locus_tag="VBC23_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6843935.1"
                     /translation="FISSSLFFIFKNSFTTTRLSFFTFIAMNSILLYLLNSFLKYYRK
                     YSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNYIAVCILDSSEKDCYDLKHNSLRII
                     NKDALTSELTCLTVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNVNVEALSFDNIG
                     EKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDITGAIIGLLICGIVAIFLVPQIRKDG
                     GPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLEDDPRITKI
                     GKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQ
                     ISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            1171..1620
                     /locus_tag="VBC23_002032"
     CDS             1171..1620
                     /locus_tag="VBC23_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6843936.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1620..2093
                     /locus_tag="VBC23_002033"
     CDS             1620..2093
                     /locus_tag="VBC23_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6843937.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
BASE COUNT          768 a          300 c          418 g          753 t
ORIGIN      
        1 tttcatatca agttcattat tttttatttt taaaaactct tttacaacga cacgactttc
       61 cttttttact tttattgcta tgaattcgat tttattatat ctattgaatt catttttaaa
      121 atattatcga aaatattctt acgctaagtt ttcacgagat accaaagttg ttttgataac
      181 gaataaggat tctttatcaa aaatgacctt taggaataaa tacgaccata attatatcgc
      241 tgtctgtatc ttggactcct ctgaaaagga ttgttatgat ttgaaacata actcgttaag
      301 gataataaac aaagatgctc ttacttcaga gttaacctgc ttaactgttg atcaagcttt
      361 tattaacata cccattgaat tatttggtaa ataccaaata caagatatta ttaatgacat
      421 tgaagcaatg ggagtgattg tcaatgttaa tgtagaggca cttagctttg ataatatagg
      481 agaaaagcga atccaaactt ttgaaggata tagtgttatt acatattcta tgaaattcta
      541 taaatatagt caccttatag caaaacgatt tttggatatc acgggtgcta ttataggttt
      601 gctcatatgt ggcattgtgg caatttttct agttccgcaa atcagaaaag atggtggacc
      661 ggctatcttt tctcaaaata gagtaggtcg taatggtagg atttttagat tctataaatt
      721 cagatcaatg cgagtagatg cagaacaaat taagaaagat ttattagttc acaatcaaat
      781 gacagggcta atgtttaagt tagaagatga tcctagaatt actaaaatag gaaaatttat
      841 tcgaaaaaca agcatagatg agttgcctca attctataat gttttaaaag gtgatatgag
      901 tttagtagga acacgccctc ccacagttga tgaatatgaa aagtataatt caacgcagaa
      961 gcgacgcctt agttttaagc caggaatcac tggtttgtgg caaatatctg gtagaaataa
     1021 tattactgat tttgatgaaa tcgtaaagtt agatgttcaa tatatcaatg aatggtctat
     1081 ttggtcagat attaagatta ttctcctaac actaaaggta gttttactcg ggacaggagc
     1141 taagtaaagg taaggtttga aaggaatata atgaaaattt gtctggttgg ttcaagtggt
     1201 ggtcatctag cacacttgaa ccttttgaaa cccatttggg aaaaagaaga taggttttgg
     1261 gtaacctttg ataaagaaga tgctaggagt attctaagag aagagattgt atatcattgc
     1321 ttctttccaa caaaccgtaa tgtcaaaaac ttggtaaaaa atactattct agcttttaag
     1381 gtccttagaa aagaaagacc agatgttatc atatcatctg gtgccgctgt agcagtacca
     1441 ttcttttata ttggtaagtt atttggttgt aagaccgttt atatagaggt tttcgacagg
     1501 atagataaac caactttgac aggaaaatta gtgtatcctg taacagataa atttattgtt
     1561 cagtgggaag aaatgaaaaa agtttatcct aaggcaatta atttaggagg aattttttaa
     1621 tgatttttgt cacagtgggg acacatgaac agcagttcaa ccgtcttatt aaagaagttg
     1681 atagattaaa agggacaggt gctattgatc aagaagtgtt cattcaaacg ggttactcag
     1741 acttcgaacc tcagaattgt cagtggtcaa aatttctctc atatgatgat atgaactctt
     1801 acatgaaaga agctgagatt gttatcacac atggcggccc agcgacgttt atgtcagtta
     1861 tttctttagg gaaattacca gttgttgttc ctaggagaaa gcagtttggt gaacatatca
     1921 atgatcatca aatacaattt ttaaaaaaaa ttgcccacct gtatcccttg gcttggattg
     1981 aagatgtaga tggacttgcg gaagcgttga aaaggaatat agctacagaa aaatatcagg
     2041 gaaataatga tatgttttgt cataaattag aaaaaattat aggtgaaata tgaggaaata
     2101 tctagattta gattattctt tattttatgc tctttgggta cttattttag taccaaacca
     2161 atggtatcag tttttaatta ttaccattat agttctatta ttactttgga agagtgagtt
     2221 tagaatatct ataagcaat
//
LOCUS       DATRME010000127         2161 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRME010000127 DATRME010000000
VERSION     DATRME010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(257..>2161)
                     /gene="ftsH"
                     /locus_tag="VBC23_002034"
     CDS             complement(257..>2161)
                     /gene="ftsH"
                     /locus_tag="VBC23_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6843938.1"
                     /translation="TTFQYYLKGTSSQNQQISYTKLVKQLKAGEIKSISYQPSGGVVE
                     VSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSVILPNDSSIKSLVSAAEENNTNIQV
                     KHESSSGTWISYIASFLPLVIMIGFFMMMMNQGGGGGARGAMSFGKNKARSSSKDEVK
                     VRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGARIPAGVLLEGPPGTGKTLLAKAVA
                     GEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAERAIIFIDEIDAVGRRRGAGM
                     GGGNDEREQTLNQLLIEMDGFEGNESIIVIAATNRSDVLDPALLRPGRFDRKVLVGQP
                     DVKGREAILRVHAKNKPLADNVDLKVVAQQTPGFVGADLENVLNEAALVAARRNKKVI
                     DASDIDEAEDRVIAGPSKKDRTISERERAMVAYHEAGHTIVGLILSNARVVHKVTIVP
                     RGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGGRVAEEIIFNAQTTGASNDFEQATA
                     MARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPEKSYSAQTAQLIDDEVRHLLNEARN
                     KAADIINENRDTHKLIAEALLKYETLDAAQIKSIFETGKMPETENDEDKARALSYDEI
                     KEKMQEEDKK"
BASE COUNT          628 a          460 c          333 g          740 t
ORIGIN      
        1 attatactaa atatttctac ttcctgtcaa tactattttt gcattttttc ttttattttt
       61 aaaaagttaa tattatttat agtaactaac cttctatact tgtttaatgg atagcatttt
      121 taccgttgtc atgttcatat ttcatcttct taattcacaa atttaaactt catcttcata
      181 aaaaacaccc ctcaaatttt atctaaattt gaagggtgtt tgaaatttat aaagttcttt
      241 aaaaatatat gatgacttat tttttatctt cttcttgcat tttttctttg atttcatcgt
      301 atgataacgc tctcgcttta tcttcatcat tttctgtctc aggcatttta cctgtctcga
      361 aaatcgattt aatctgagca gcatcaagag tctcatattt taagagggct tctgcaatta
      421 atttatgagt atcacggttt tcgttgataa tatcagctgc cttattacgt gcttcattaa
      481 gaaggtgacg aacttcatca tcaataagtt gtgcagtttg agcagaatat gatttttcag
      541 gtgacatttg gccagccatc attgcatggt taccttcgta ttgaactggg ccaagtttct
      601 cactcatacc atattcagtt accatagcgc gggccatggc agtggcttgt tcaaagtcat
      661 ttgaggcacc tgttgtctga gcgttgaaaa taatttcttc cgctacacgt cctcccataa
      721 gacctgctaa ttgctctttc atatcatctt ttgaaagaag catttgatct tctttaggta
      781 aagcaatcat atatccacct gcacgaccac gtggtacgat agtaacttta tgaacaactc
      841 gcgcatttga aagaatcaaa ccgacaattg tgtgcccagc ttcatggtaa gcaaccatag
      901 ctctttctct ttcagaaata gtacgatctt ttttagaagg accagcaatt acacggtctt
      961 ctgcttcatc aatatctgaa gcatcaataa cttttttatt tcgtcgcgca gcaactaaag
     1021 cagcttcatt gagaacattc tccaaatcag caccaacaaa tcctggggtt tgttgagcta
     1081 ctacttttaa gtcaacatta tctgctaatg gtttattttt agcatgtact ctcagaattg
     1141 cttcacgacc ttttacatca ggttgtccaa ctagaacttt tctatcaaaa cgtcctggac
     1201 gcagtaatgc gggatctaac acgtcactac ggttggtagc agcgataacg ataatacttt
     1261 cgtttccttc aaaaccatcc atttcaatta aaagttggtt gagcgtttgt tcacgttcat
     1321 cgtttccgcc acccatacca gctccacgtc gacgtccaac agcatcaatt tcatcaatga
     1381 aaataatagc acgttcagct tttttagcat cttcaaacaa agagcgtact cgactagctc
     1441 caacaccgac aaacatttca acaaaatctg aaccggaaat actaaagaat ggaacccctg
     1501 cttcaccggc aacagctttt gctaataggg ttttacctgt ccctggaggt ccttctaata
     1561 gaacccctgc tggaattcgg gcacctagag atttatatcg tttaggatct ttaaggaagt
     1621 caacgacttc tattaattct tgcttttctt cctctgctcc agcaacatcc gaaaatcgaa
     1681 ctttaacctc atctttacta gatgatcttg ctttattttt accaaagctc atggcaccac
     1741 gagcgccacc gccaccgcct tggttcatca tcatcataaa gaaaccaatc atgataacta
     1801 aaggtaaaaa actggcaata taagaaatcc atgtcccact tgaactttca tgtttgacct
     1861 gaatattagt gttattttct tcagcagcgc taacaagaga ttttattgaa gagtcatttg
     1921 gtagtataac agaattaaat cctgtaactt tagtagcaac actaccacct aaaaaagtaa
     1981 aactattagc tgatttaatt gttttggctt tcttatatgt cccactaact tcaacaacac
     2041 caccacttgg ctggtatgaa atagacttaa tttctccagc ttttaattgt ttaacaagtt
     2101 ttgtataaga aatttgctgg ttttgagagc tagttccttt taaatagtat tgaaaggttg
     2161 t
//
LOCUS       DATRME010000128         2137 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRME010000128 DATRME010000000
VERSION     DATRME010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1367)
                     /locus_tag="VBC23_002035"
     CDS             complement(<1..1367)
                     /locus_tag="VBC23_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6843939.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRG"
     gene            complement(1360..2037)
                     /locus_tag="VBC23_002036"
     CDS             complement(1360..2037)
                     /locus_tag="VBC23_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6843940.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
BASE COUNT          690 a          424 c          290 g          733 t
ORIGIN      
        1 ccacgctttt tgataaaatc ttttaattgt tctactgatt tgtgataact tgtcacattg
       61 cgttcagtag gaatcaaata attccttgga tcagtattct ttaaatcaaa gacatagccc
      121 cattttttat aatttcgatc aatagctgga tctaaaaaat gccttgtctc gtctatgtaa
      181 ttagataaat attctgtcgc tagaactccc tttcgtaatt ctttatagcg atgaacaacc
      241 aggtctataa atgctttgtc cttaatgagc atattgaacc acggtgcatc cgtcaaggta
      301 aaatctgcct catcaacacg tccttcaatt tgattatcaa aagcattatt aaaatcccaa
      361 actactagtt ttgctctatc acgaagatct ttatatagat aggttgaaaa tttacccgca
      421 tccacatttc taaaaaattc attgatgaca aaataatttg caaatgattc tctatcaata
      481 tatttaggat attgtgaaaa atcataagaa taaaggactt tttcaatatg attgatgtcc
      541 ttattaataa actctaattg ttttgatgtc aatctttgtt tacctggata tttcacatca
      601 agagctgaga taccagactg gtgtgtataa tgtacatagt tatctaactt ttgctttgcc
      661 ttatgttcac gatcccaagc aacgatataa ggtgttttat gcaacttttt atcacttttt
      721 tcaatgggaa ctcgttgctc accttgttcg atattctcaa ctgctagata tactccctga
      781 tactcaccat tgacaaataa ctcacagtag cgaacgtttg gggcatagga cataatctca
      841 ccagcaatat tataactcag ataatttctt aataatgttc tgtctagaaa gggaccatgc
      901 aatacccact ccgattcttt aggcattcct gctaatcgat gcttcttttc ctttaactta
      961 ttagtaacaa acttaacttt caatgacttc ttatcaaagt aacgagagga atttcctcta
     1021 tatgatatta acccttttgt cctaattctc ggcttttctg ataaatgatg tgatctactt
     1081 ggagaatccc gtaattcgat atcaacatta atattatccc tagctttgac atattttccg
     1141 ccttcttttg taacaagagg aataacttgt tgctttgtat caatgctgat aatgggcaaa
     1201 tgggttttaa atcctgttaa atcactcttc gcatctacct gctttatacg atgatgatgt
     1261 attctctcga tgtgatgctt aggaatccaa aaatagaaaa ataagaatag aaaaagacct
     1321 ataagggttg tgccgtaaat aaccttccct ctcataagtc tacccattga tttcgtcgct
     1381 ttggctaaca atgttgaaat aatcaatatt tcccaattgg tacacttttt cagttaactg
     1441 attatctaca tggagttgct tatcgtaatc ttttctagag atttcgaaaa tcatttcaat
     1501 agtattagtt gttgaatttt taacacgctg aacagctttt ccgtcaaaat attggaagaa
     1561 aattccttct aaatcaactt ctagtgttct atcgcacttc acaatcaata acatacgatt
     1621 ctcgttttta acacgtccca taacccataa taagataaag ataacgctac ttcctaatgc
     1681 agctaccaca tagtcaccga caccacaaca gataccaaca actatggtcc aaaaaatata
     1741 aactgtatct cttgaatctt ttatggctgt cctaaaacga acaactgaca aggcaccgac
     1801 catacccaat gacaaggcta cattattacc aataacggtc attactgttg cagtcaagac
     1861 cgtcaatgtc attaatgaaa cattaaactt tttactatag gcagttccac tatgcgtata
     1921 agcataggaa acataaataa ctacactcaa taaagctgct acaagaatat gtaaaatcat
     1981 atcttgaaaa cttaaatttc cactttgctc tagactattt cgtaataggt ctttcatttt
     2041 ttgttatcct ctcacattta ataataatta acgtatatac tttttcacta aaaacgataa
     2101 tgtttactaa cagctcttcc taaacaatac ttactcg
//
LOCUS       DATRME010000129         2072 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRME010000129 DATRME010000000
VERSION     DATRME010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..151)
                     /locus_tag="VBC23_002037"
     CDS             complement(<1..151)
                     /locus_tag="VBC23_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6843941.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYL"
     gene            complement(351..770)
                     /locus_tag="VBC23_002038"
     CDS             complement(351..770)
                     /locus_tag="VBC23_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6843942.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            complement(990..1543)
                     /locus_tag="VBC23_002039"
                     /pseudo
     CDS             complement(990..1543)
                     /locus_tag="VBC23_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
BASE COUNT          724 a          363 c          245 g          740 t
ORIGIN      
        1 ataaatacgg atgctcttca agcaacctaa aaagtacaat acgtccttct tgaatccaat
       61 catcatattc ccaaagttga acaaaataat ttcttctcag cttcattacg attggcttta
      121 ctttatcaaa caactcctca aaatctctca aaacaactta ttcctttctt aaacatatac
      181 taagtataaa aaaagtaagg tgataacaac atgacaatgg tgtcctttaa ggaaatgatt
      241 ttttcccaaa acacattaat tgagcattat cagactattt agtaactcat attagttaac
      301 atataaaaag cttgagatga tactctcaag ctttttatat gatgacacaa tcaatgccct
      361 tttttctttt ggtaacgttt ttcttgtttt aattggtacc gatgctttct taactcattt
      421 ttcttacatt tctgacttaa ttgacgttcg ttttttatac tcatatgaat agttttcatc
      481 gccaattgcg cttttgtaga tactactggc ttacgttttt ctctattaat ttctctttgc
      541 attcttttag gacttttttt gtgttcattc gtacgcttta gagaaatatc cgttttaaca
      601 aactcgtatt tttttatcaa atcattaagt ttatgattta taaaattaaa aacatcatca
      661 tctttaggtt cttttccaaa aaaataccga aaaactttgt aatcaccatc atcatcgtac
      721 tcaatcaaac ccaaccaaaa attaccatca aaatagactg tcattttcat acagccccct
      781 cctttaaata ttaaattgca atggacgacc tggagggaag gttactgacg ttcactgaac
      841 gcttgcagac taccaactgc aacgtgtttt tatgctataa cgattataac acagtacact
      901 atgattatcg ataaacgacc ttattaaagg tctatactat ttgtcagtct aaaaatttaa
      961 aagctgttaa gccaactcca gtaacgttat cattttttta tgattgttaa acgaggccaa
     1021 atattattcc atttcttctt atccactcgc ttcaaataaa cattataacg taatagatcc
     1081 tcttcaaaat atggcgtagg actaacaatg aaatttaata tttcctcttc accataagag
     1141 ggaggtaaag ttcgagttga tttctatcat ctaacctttc tcccactgca gtacactttt
     1201 caggaaattt cgaaatagca tctttagagc tagtatactt tggggtatta ggagaatgag
     1261 tgttcatgta aaattcattt ttaagttccc aatcgtactg tggatagtta tcctttaatt
     1321 gttgttctaa taccaccgtt tcttcataag aaatattttt atcaaaaaat acaacatcaa
     1381 tgtctgatgt caatgtctca tttattccag aaagtttatt ccatataaag tttctcaatg
     1441 tgccagcaca cagccaacaa tcattcagtg gaagagattt tattatagct aaaatcttca
     1501 ttatatcaga attacataaa atcatatgat aaattttagt cattttatct cctttgctac
     1561 atctccagac aatgtttaaa ttttaatctt attatatacc attattagat cagatagtaa
     1621 gatcaacaag aaatacatcc ttaaatttta aacaattttt agttattaca atcacgtgat
     1681 tgaatgctcc aagtgtcaat ttaagataat tattatccca tatattaagc aagaagaata
     1741 atcatcaagt taacatatta aatcaaccag ataaagggat cgcttttgat gaaaagaatt
     1801 acattgatga tttattgaca aactggaata ttaaaagtat catttgatac cagaataatt
     1861 acataattaa taaaccatta ctcagaaaga ataaaaacaa caatattgtt atctcgccta
     1921 atacaaacaa taaacttaca tttagttcac taaaaatatt tagcctattt catatgtaca
     1981 aggttatcaa aaggatattt aaaactatgt accaatatat gacgttagat actaggttaa
     2041 aatattttaa cttctacagt tcttttaaag at
//
LOCUS       DATRME010000130         2017 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRME010000130 DATRME010000000
VERSION     DATRME010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            274..1860
                     /locus_tag="VBC23_002040"
     CDS             274..1860
                     /locus_tag="VBC23_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6843943.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
BASE COUNT          679 a          330 c          410 g          598 t
ORIGIN      
        1 actaaggaga ttccaagtga agcagacgtt gcgttctaga tagataaact atgaaccctc
       61 cttctttata gatggagggt ttttattgcc ttttttctat tttctgaagc aactaataga
      121 actagcaatt tatgaaagcg tgttatttat tagattgacc ttattttata tctttttact
      181 tttaattgtg ttacactaag taggttgtca gaaaagataa ctaataaagg ttcataaggc
      241 agcttcgcac tgtcttttag aaaagagaaa catttgaaat ttacagaact taacttatcg
      301 caagatattc tttcagcggt agaaaaagca ggctttgtag agccatcacc aatccaagaa
      361 atgactatcc cacttgctct tgaaggcaaa gatgtcattg gtcaagcgca gacaggtaca
      421 ggaaaaactg cagcttttgg gttaccaact ttaaataaaa ttcatactga agataatact
      481 attcaagcac ttattattgc tcctacacgt gaacttgcag tacaatcaca agaagaattg
      541 ttccgttttg gacgcgataa aggtgttaag gtccgctctg tttatggtgg gtcaagtatt
      601 gaaaaacaaa tcaaggcatt acgttcagga gcacacgttg ttgttggaac tccaggtcga
      661 ttacttgatt taattaaacg taaagcactt aaacttaatc acatcgaaac tcttattctt
      721 gatgaagcag acgaaatgct taacatggga tttttagagg acattgaagc aatcattagt
      781 cgtgtaccag aagcacgtca aactctttta ttctcagcga caatgcctga tccaatcaaa
      841 cgtatcgggg ttaaattcat gaaagatcct gagcatgtta aaattaaagc aactgaatta
      901 actaatgtga atgttgatca gtattatgtt cgcgttaagg aaaatgaaaa attcgatact
      961 atgacacgtt tgatggacgt tgatcaacca gaattgtcaa ttgtctttgg acgtacgaaa
     1021 cgtcgtgtag atgaattaac tcgtggtcta aaattacgtg gtttccgagc agaaggaatt
     1081 catggagact tagatcaaaa taaacgtctt cgtgttatca gagactttaa aaatgaccat
     1141 attgatattc ttgttgccac agatgttgct gcgcgtggtt tagatatttc aggcgttaca
     1201 catgtttaca actatgatat tccccaagat ccagaaagtt atgttcaccg tattggacgt
     1261 actggtcgtg ctggtaaatc tggtcaatct atcacttttg tttcaccaaa cgaaatggga
     1321 tatttgacaa ttattgaaaa cttaactaaa aaacgtatga ctggaatgaa gcctgctact
     1381 gcaagcgaag catttcaggc taagaaaaaa gttgcattga aacgtattgc acgtgacttt
     1441 gaggatcaag aattagtttc taaatttgat aaatttaaag ccgatgcttt agaattagca
     1501 actcaatata caccagaaga acttgctctt tatgtcttaa gcttaacagt tcaggatcca
     1561 gaatcgttac cagaagttga aatcacacgt gaaaaaccat taccatttaa accatcaggt
     1621 ggtggcttta aaggtaaagg tggacgaggt aatggccgtg gtggcgatcg tcgacgcaat
     1681 gatcgtggtg atcgtcgtgg aaatcgtgac cgtgatgatc gtggaggtcg tcgtgatttt
     1741 aaacgtcgtg atgataagtt caagaaagat aatcgtcgtc aagaaaataa aaaacctcat
     1801 aaaaacactt caagtgaaaa acaaactgga tttgttattc gcaacaaagg cgataaataa
     1861 gaggattacg aaaaaggagt tgctgagcga ctcctttttt attttttaag ataaagcgta
     1921 gatagtgata tccgtaaaat tgccttataa ctttaagaat gaagacaaaa gattaaagtg
     1981 aggttgaaat ttaaaagtta aagaagcgag gacaggc
//
LOCUS       DATRME010000131         1987 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRME010000131 DATRME010000000
VERSION     DATRME010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            235..1041
                     /locus_tag="VBC23_002041"
     CDS             235..1041
                     /locus_tag="VBC23_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6843944.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1124..1627
                     /locus_tag="VBC23_002042"
     CDS             1124..1627
                     /locus_tag="VBC23_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6843945.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1731..1907
                     /locus_tag="VBC23_002043"
     CDS             1731..1907
                     /locus_tag="VBC23_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6843946.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          722 a          267 c          385 g          613 t
ORIGIN      
        1 agcaactgta caaggttctg aaagttagtt gattattaat gatagttagc attttttaag
       61 aattggaata cttttaaaag gtgattgttt taattagtta tagttgttat taaaaatcgt
      121 ctaacagcaa gcatagttat cggtgtaatt tactgaattt ttattcaaaa aaacttgact
      181 tatgtataaa gatgatttat acttgttcct atcataataa aaggaagata gtttatgtca
      241 aaaaaaataa tattaggaat tttatctctt ttatctgtcg ttactttggt ggcgtgtggt
      301 tcatcagaca aacagctaca agataaagtt gagaaaaaag ggaagttagt tttagcggtg
      361 agtccagatt atgctccctt tgagtttaaa gcgcttgtta atgggaaaga taaagttgtc
      421 ggcgcagata ttgagttagc tcaagctatt gctgatgagc ttcatgtcaa actagaggta
      481 tctcctatga gttttgacaa tgttttatca agtttacaaa caggtaaggc tgatatggct
      541 atttctggat tatcatatac taaagagcgt gctaaagtct atgatttttc aaaaccttat
      601 tacactacag aaaatgcggt tcttgttagg aagtctgatt taggaaaata tacttcaatc
      661 gaaagcctta aaggtaaaaa aatagcagta caaaaaggaa gtatcgaaga gggagtttct
      721 aagaaatcat tcaaaaattc tcacgtcgtg tcactgacag cgatgggaga agcgattagt
      781 gagttaaaat caggcaaggt agaagctgtt gatttagaaa aacccgtagc tgaaggttac
      841 ttggcccaga atccagacct tgttcttgct aagtttgccc ttaaaacagg agaaggggat
      901 gctaaggctg tagctttacc taaagacagt ggaaaaatgt tgaagactgt taacaaagtg
      961 ataaagagac ttgaaaaaga agataaatat aaagtttata tttcagatgc ggctaaatta
     1021 actggcaatc aagttgaata atattaaaaa gcctagtcat aggctttttt ggatgttata
     1081 atatggcttg actaagaata atagcataaa ggcagaggaa tatatggttc agggttcaat
     1141 taatattgaa aaatataaag atttggcgct ccaaaaacaa ggcgagcatc gtaaattttt
     1201 agcaagttta aaaaagaaag ctcctaaaaa cttagataaa atagtccaac agatacatca
     1261 agaggttttt caagaaattg attgtacagc atgtgctaat tgttgtaaaa ctttgggtcc
     1321 tctatttaca gaagctgata tttctaggat ttctaaacat ttccggatga agttatcggc
     1381 ttttgaagat ttatttttag aagtagatga agatggagat aaagtattta aagcgatgcc
     1441 ttgtccgttt ttaggcgatg ataacttatg tagtatatac gatgtcagac ctaaagcttg
     1501 tcgtgaattt ccacatactg atcgtaagaa aatctatcaa attaataact taacgataaa
     1561 aaatactctt acttgtccag cggcatacct ttttgtggaa aaattgaaat cacgtatagg
     1621 gaaataataa cccagagaaa gtatcataaa aatattgaca atctgctttt atttgggtat
     1681 cctagtaagt gaacgtgaaa atgttacaat atcttgagga ggtgaaacat atgtcaaaaa
     1741 cagtagtacg taaaaacgaa tcacttgacg atgcacttcg tcgtttcaaa cgttctgtga
     1801 ctaaagctgg tactcttcaa gaatcacgta aacgtgaatt ctacgaaaaa ccttctgtaa
     1861 aacgtaaacg taaatcagaa gcagctcgca aacgtaaaaa attctaatta gatagaatat
     1921 gaaaaaacag ttcagaattg agctgttttt tcatatatta taactataag ttaataaaaa
     1981 aactcgg
//
LOCUS       DATRME010000132         1968 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRME010000132 DATRME010000000
VERSION     DATRME010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            98..398
                     /locus_tag="VBC23_002044"
                     /pseudo
     CDS             98..398
                     /locus_tag="VBC23_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            638..841
                     /locus_tag="VBC23_002045"
     CDS             638..841
                     /locus_tag="VBC23_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843947.1"
                     /translation="MAESANTVRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKGN
                     QITQGGSNLPNDGSVYLQPNKSL"
     gene            992..1432
                     /locus_tag="VBC23_002046"
     CDS             992..1432
                     /locus_tag="VBC23_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO6843948.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            1425..1769
                     /locus_tag="VBC23_002047"
     CDS             1425..1769
                     /locus_tag="VBC23_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843949.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT          763 a          233 c          365 g          607 t
ORIGIN      
        1 tgtatatgtt acgactaaaa aattaaactc gtgaaaattt gttatactaa tattagtttt
       61 aaaatactgg taaataataa ttatttttat tagatacttg acatgggaag tttattttct
      121 aagaaaagag agcatgatga aaaaatatgt aaaatggtta attcccatat ctattattgg
      181 gatgttacta gcaggttgtc aaatgaatag tgaatctaaa agtcagtcaa acgaagtaaa
      241 aaatagcaaa cagtcagcag taaagaaagg taaaaaagta ataaaaaaag aacaagtagc
      301 ttatctcaaa aagtatgaaa aagaattaac tgaatatgtc aaaacccaaa atccagatat
      361 tagcgaaatt aagtatgatt ggaatagtgt gcgtatagtg gaagctagta atggaacccc
      421 tcaaggcgga gagcagctat tattaatttg gggttatgca aatggcagtg aattaaccaa
      481 ttttactcta aatttcacat tgggtgataa tcaaatgcct aatattgact cagttggctc
      541 agataattta tcaactattg aagattaata ataacagtaa gaaaggtata taatgggatg
      601 ttggaatctt aaggaatttg aagatatatt ttgaagtatg gcagaaagtg ctaatacagt
      661 tcgatcgaat tcttttgttt tagaagaatt aactgaattt caaagagaaa agttaaaaaa
      721 tggggagtca gttaagttta atttttctaa agatgttaag aaaggaaatc aaatcaccca
      781 aggtggctca aatctcccaa atgatgggag tgtctatctt cagcctaata agtccttata
      841 aactgtttat taaactgaaa aaattgaagt tttcatagat aaaattcttc gagtaaaata
      901 ttagagatgc tatttatatt tcttaagcta gtaatttttt attgtttatt ggtttaatga
      961 taatacaaat ataaaatagg tggtgcaaat aatgaaaaaa catataaaaa gtatcatacc
     1021 aatagttctt attggtatga tactaggagg ctgtcaaatg aatagtgaac ataaaagtca
     1081 gtataatgaa acaaaaagta gcaagcaatc agaagtgaag aaagataaaa aaatgacaaa
     1141 aaaagaacaa ttagcttatc tcaaagagca tgaacaagaa ataattgatt ttgtaaaatc
     1201 tcagaataaa aagatagaat ctgtacaaat tgattggaat gatgttcgat ggagtaaagg
     1261 gggaaatggt acacctcaag gaggaggaga ggggatttta ctttttgggg agattaataa
     1321 tgattctgaa tcaagttgga gagttgatat tgatatagaa aaaggacggc tagacctaaa
     1381 aaatatgtat ttaggacaac ctatacgaat tggaggtaaa ttatttgagt aattggaact
     1441 tagaaagttt tgataaccta tttgctactt tagcagaatc aacatacaca aaaagaccaa
     1501 gtaactttac atataatgaa ttgaatccta agttgctacg acaactgaac tcaggtagtt
     1561 ctgttgaatt ggatttctca aaatctgtta agaaaggaaa aaaagttatc gaaggcggtc
     1621 agaacctccc caatgatggt attgtctacc tgcaaccaga taagtcctta aaatctattg
     1681 atgaaaaagt agaggttcgc gttcctgatg tcaatggcgg ttatcactct gagcattgtg
     1741 ttactaattc ctatcagaaa ggttactgaa agatgatgaa gcagtggctc tataatcact
     1801 aaaagctcat cattgctcag tgtttaagtt aacttctaaa gctcgaacct tgttaaatca
     1861 aggtttgttt acttttaaaa gtgacatttt atttttctaa aaataaggtt atcgggattc
     1921 ctcattctaa gttactttgt ttatatgtac ctttacttaa ggtttatt
//
LOCUS       DATRME010000133         1955 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRME010000133 DATRME010000000
VERSION     DATRME010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(3..1028)
                     /gene="trpS"
                     /locus_tag="VBC23_002048"
     CDS             complement(3..1028)
                     /gene="trpS"
                     /locus_tag="VBC23_002048"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6843950.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(1136..1828)
                     /locus_tag="VBC23_002049"
     CDS             complement(1136..1828)
                     /locus_tag="VBC23_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6843951.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT          651 a          378 c          278 g          648 t
ORIGIN      
        1 tattatttaa aatagttcaa acccatggct gatttaacct catccaaagt agaggctgca
       61 acagcttgag ctttctcact acctttttgt aacatttgat aaacttgtcc catatcttta
      121 gcatattcaa ggcgacgttc tctaataggt gataattcac gctctaagat atcaagtaga
      181 taacgttttg ttttaacatc tccaagtcca cctttttgat agtgttcttt catagcagta
      241 atctcttttt ggtcttcatc tcttccaaaa acatcaaggt aatggaatac catattacct
      301 tcgatttgtc ctggttcttc tactttaata tggtttggat cagtatacat gctcataact
      361 tttttcttaa ctgtatccat atcatctgct aaaaagattc cattatttaa tgacttagac
      421 atttttgcat taccatctaa ccctggcaga cgacctgctg catcattttc tggatagatt
      481 ccctctggtt caactaaaac ctgacaatta taagcatgat tgaaagatct aactatttca
      541 cgtgtttgtt caatcattgg tttttggtca gtccctacag gaacaaggtt agctttaaaa
      601 gcggttatat cagctgcttg tgcaacagga taaactaaga atcctgctgg aatagactca
      661 ccaaaacctt tttgagcaat ttctgttttt acagttggat tacgttccaa acgtgcaagt
      721 gaaaccaagt tcatataata catacttaat tctgctaact caggaatttg actttgaatg
      781 aataaagtcg acttgtttgg atccagccca acagctaaat aatcaagtgc aacattacca
      841 attgattcaa caatagtttg cggatcttta gcatggtctg tcagtgcttg ttggtctgct
      901 aaaaagacaa ataaagtata gctaccctca ttttgaagta gaactctatt ttttaagcta
      961 cctacataat gtccaatatg caactttcct gtcggtctat cacctgttaa gataattggt
     1021 tttgtcatgt ttactcctta atataccaaa gatatcattt catatagtta tcttatcatg
     1081 tcacaagccc tattgcaatt catccctaca agaaacattc accttattaa gatgattaaa
     1141 cattatgtaa cgttaaatcg ttcttaataa tttcaatcgt ataatgaatc ataataatta
     1201 aaagcgttat cgtcacacct aaaatagctc tatatgcaaa gatatcgtaa actattgcag
     1261 ctaccaatgc tccaataata aatgctagaa ctaccagtga ataattgata gctcgtcttt
     1321 tttgcatagc cgttctctga cgattaacaa tatactcata ccaaaattgt agcattttcc
     1381 gataattacc agaagtcatt aagatagtgt atggttcaga ttcaatgcga cttcctgaaa
     1441 atgtcaacat taaaatacct gtgctaaagg ctaataaagc tacccagata ttgtggttta
     1501 cattttcaac aaaaggaata aacatcgtag aaagtaaaag gggacataat gcatatagac
     1561 gccaaaaagc tgttactgca cgttctttaa tcacaatacc aaccaaaaaa ccaattgaaa
     1621 aaaagaaaat agaaaataat ttagtgataa aaaagtgaaa atcattttgt ccgagatttg
     1681 ccaccatcaa gataatatta cctgtctgtg ttgcaacaag ggagtgaaac tgcatgtgac
     1741 tataaacgtc aataaaacca ccaataaaac ccaataatac agccattttt cttccatctt
     1801 gtggcattat aatatcatct ctgcgcatta tagcaaattc tccttcttgt attatcatgt
     1861 tttctattat ataccaataa aataggcatt caactcaaaa atatcgagaa atttcttttc
     1921 taaatttact tgactaccaa gtctcgctgt tagat
//
LOCUS       DATRME010000134         1865 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRME010000134 DATRME010000000
VERSION     DATRME010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            92..952
                     /locus_tag="VBC23_002050"
     CDS             92..952
                     /locus_tag="VBC23_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6843952.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            995..1726
                     /gene="yaaA"
                     /locus_tag="VBC23_002051"
     CDS             995..1726
                     /gene="yaaA"
                     /locus_tag="VBC23_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6843953.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
BASE COUNT          664 a          334 c          269 g          598 t
ORIGIN      
        1 taatataagc tatatttcaa aacaaattag tttatttctc tcaatgaacg gtttaatatg
       61 ttactatata gtaataatag gaggattaac tatgccgtat ccatacaaag ccaatcattc
      121 cattgaatca atcaccttaa aagtaaatga cctcgaaaac ctcgtaaact tctatagcga
      181 tatcattggg ttaactgtta ttgacaaatc aagcactaga gccttactag gggtaaatca
      241 aaaaatccct cttattatcc ttgaaaaaac tgaacttgaa aagcatagta cctatggcct
      301 ttatcacact gctattttag taccagatga ataccatctc agtctagctc taaatcattt
      361 attatctcag catatccctc tagaaggtgg agctgaccat ggctatagca atgctatcta
      421 tctaagtgat cctgaaggta atggaattga aatttataat gataaggata tctctatgtg
      481 ggatatcaga gagagtggac aaattattgg tattactgaa agactagata ttgacaattt
      541 acttgacagc cttgtcaacg ttcctaataa ctataagctt tctgaaaaaa ctcgtattgg
      601 tcatattcat ttgagtgtta aagatgctaa gattagctca aaactctatc aaaatgtttt
      661 tggccttgat gagaaatttg caatcccgac agctagttgg attgcatccg gtaactacca
      721 tcatcatcta gcctttaata attgggctgg tccaaatctt tctaaaaacc aagaaaatag
      781 acccggaatt tccttattga caattgctta caatgatgac aaccttttta gagatagctt
      841 aaaaaaagcg caactttacc aactgacttt ccttgaaaaa caagatcatt attatattat
      901 tgaagatttt gatggcatcc gaattaaagt tgttctaaaa ccagagaatt aaccatcact
      961 tgacttttga ctcttcattt tacataatgt caatatgata aaaattttga tcccaactgc
     1021 aaaagaaatg aaagtgtgtc aaaacattgc aaggcctaaa ctgagtgcac aaacgcaaat
     1081 tataattgat tatttttcta ctttaactgt ttcagactta gaggatatct acagaattaa
     1141 tacaagtgca gctcgttgtg aggctcaaag atggcaagat tttaaatgta aacaacttac
     1201 tctgaatcct gctattaagt tattcaacgg tcttatgtac cgcaatatca aaaggcacaa
     1261 cttatccaca agtgaagcac aatttatgga aaattctgtt tttattacta gtgctcttta
     1321 tggcatcatt cctgctatga cactaatatc ccctcatcgt ttagatttta acactaaaat
     1381 caaaatcaac aataactctc ttaaagtttt ttggcgtgaa aattacgata cttttatgca
     1441 gtcagatgac attatggttt ctttgctttc taatgaattt aaaaccgtct tttctcctaa
     1501 agagcgtcaa aaactaatac atcttaactt tattgaagat cgagatggcc aattaaaaac
     1561 ccactctacc atatcaaaaa aagcaagagg aaagtgttta acttctatga tggaaaataa
     1621 ttgccaaact ctagaacact taaagcaatt acgctttgat ggcttctgtt atgataatga
     1681 actctcagac tctaagcaat taacttttgt taaaaaacag acttaagtaa ctatttatcc
     1741 acaatttatt cacacaaaaa gcaagagata atcgtatctc ttgctttttg acaaacttgt
     1801 aaggtctaaa aacctctcta tatcaaggtt tcaaataata ttaacaaaaa ataattgtta
     1861 tcaac
//
LOCUS       DATRME010000135         1811 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRME010000135 DATRME010000000
VERSION     DATRME010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..351)
                     /locus_tag="VBC23_002052"
     CDS             complement(<1..351)
                     /locus_tag="VBC23_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843954.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIYHNEGNFVVTNIDTKSKSFVLKGGSAKYTVLYDVKSDGKLNVDLGNFYYNAS
                     GKETYYKEGSKALEK"
     gene            complement(504..692)
                     /locus_tag="VBC23_002053"
     CDS             complement(504..692)
                     /locus_tag="VBC23_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6843955.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(689..982)
                     /locus_tag="VBC23_002054"
     CDS             complement(689..982)
                     /locus_tag="VBC23_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6843956.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            1039..1188
                     /locus_tag="VBC23_002055"
                     /pseudo
     CDS             1039..1188
                     /locus_tag="VBC23_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1694..>1811)
                     /locus_tag="VBC23_002056"
     CDS             complement(1694..>1811)
                     /locus_tag="VBC23_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6843957.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          651 a          337 c          190 g          633 t
ORIGIN      
        1 tttttctaaa gccttgctac cttctttata atatgtctcc ttaccagaag cattataata
       61 aaagttccct aaatcaacat tcaatttacc atcactttta acatcatata aaacagtata
      121 cttagcagac ccacctttaa gtacaaaact tttactttta gtatcaatat tagttactac
      181 aaagtttcct tcattatgat aaattcctga ttcatcatta atttctaaaa ctaacatttt
      241 ttcaccatca tatactttat aatactttac atttaacgaa ttagtcgaac aagcaactaa
      301 aaatactgta gatatagcta aaatcatcaa tcctaataat ttctttttca taactaaact
      361 tcctttgcta tgagaaaatc ttttaacctc tcaacatctc tatcgcttaa cttacctata
      421 attttaatat tgaacccttt attctcaaca tcataatatt gaatagtatc tatccaagaa
      481 cgcctattta aatttgcttt tgcctaatca ataacttcta aatactgact tcttatattg
      541 tcagacttat tttcatattt agtagtaaat cttaaagttt ttattacttc atcattaaat
      601 ttgactacta cagcaggacg aacctttgaa cctgtaccat cagaatattc aatacgagaa
      661 actaatacag aatatacctc aatatcactc atttaaaata ttcctcatat tctgaaaaat
      721 cttcatctcc aaaaaacgca tttacacttt ctggatcact agccttaact cgtattccct
      781 ttaattttgt ttctaccaaa ttttgaaatt cttcatcaga aacagatgat ttatttttta
      841 tttcaaaagg aatactttga gttttaacag attgaatcaa aaacattttt actgctgtgc
      901 tcatatccaa ccctaaggat tgaaaaagtt ctgtagcatc atcttttaat tgatcatcaa
      961 ctcttactgc tactgttgac ataatatcac ctcaaatcat ttttgtataa ctattgtatc
     1021 actaaatgca atacaacgca aacaaaaaag gaaatcgtct ttcccaatga agaggctcta
     1081 gaatactatc tagtcacact atttaatgat tacaacttca agcaagaaca acgaatccac
     1141 aaaggttttg gccaatgtac agacacactt gaaagcctat ttgattaaca atcccagctt
     1201 tgccagtgtg tttacacaac attattgaca cactcctttt atttttataa attaccctta
     1261 aatacattta attacttgaa aagacaaaca gtcattttag aaaattttgc atctaacctt
     1321 acatttgatt acacctaaaa attttaaatc atcaaaagag agcctaggat taacatctta
     1381 ggctctaatg atacacttca tttacaaatt tcaactaaat aggatcattt cttcagtttt
     1441 atagtaagcg tccaataaca aggtatattg gatgcttacg gaatagggaa gaaaatggaa
     1501 gatacctcgt tattggacgc ttacacaaaa tcaaaatcct atcgaaatta ataatctcga
     1561 taggattttt caagaattaa actcgaaaaa ttgaaacact ttaattaagt ttgttgaaga
     1621 gagatacata actttattaa tgtgttcaca ccaataaatg gtgaaaagtt tagtgacatt
     1681 ttaggtcaaa agattaatcc tcttttttct tagaaacaga taataaacca actgatgaca
     1741 taattgtcaa agctacaaca ttaaagaatg gagttgcatt ctcacctgtt gctggtagtt
     1801 tatttccttt a
//
LOCUS       DATRME010000136         1732 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRME010000136 DATRME010000000
VERSION     DATRME010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..330
                     /locus_tag="VBC23_002057"
     CDS             <1..330
                     /locus_tag="VBC23_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843958.1"
                     /translation="QYPIPSELTFIKFYGHGLGEADYSYFQSIFDSINLYSSDTVLIF
                     YWSCYNENIREQIKSQQVHAVHKLIEKYGDTFNNKNHGRNLFTKLQLENRLIIQEILY
                     NDILNPY"
     gene            complement(465..830)
                     /gene="rplL"
                     /locus_tag="VBC23_002058"
     CDS             complement(465..830)
                     /gene="rplL"
                     /locus_tag="VBC23_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6843959.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(894..1394)
                     /locus_tag="VBC23_002059"
     CDS             complement(894..1394)
                     /locus_tag="VBC23_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6843960.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1424..1557)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          550 a          315 c          262 g          605 t
ORIGIN      
        1 caatatccaa ttccctctga actaactttt ataaaatttt atggtcatgg tctgggtgaa
       61 gcagactatt cttattttca atcaattttt gactcaatta atctttattc cagtgacact
      121 gtactaattt tctattggag ttgttataac gagaatatta gggaacaaat taaatctcaa
      181 caagttcatg cagtacataa attaattgaa aaatatggag atacttttaa taataagaat
      241 catggcagaa atttattcac aaaacttcaa ttagaaaatc gtttaattat tcaagaaata
      301 ctttataatg atattctaaa tccatattaa acaaaacacc cccacaaaat attgtggggg
      361 tgtttgtggg gaagattcaa ttgaatcttt ccttttttaa tcgccttaac tttaacgaaa
      421 agactttaaa actaggcttt cacctttaaa caaccaatag atgattattt aagagtaact
      481 gaagcacctg cagcttcaag tttttctttg atttcgttag cttctgcttc tgaagcacct
      541 tctttgataa ctgatggtgc gttatcaacg atagctttag cttctttaag accttcgcct
      601 gtgatttcac gaacaacttt gataacgcca acttttttgt ctccagcagc tgttaattca
      661 acgtcaaatg agtctttagc agcagcagct tcaccaccag cagcggcagc agctacagga
      721 gcagcagcag ttacaccaaa ttcttcttcg atagctttta caagatcgtt aagttcaaga
      781 attgtagctt ctttaatttc agcaataata ttttcaatgt ttaatgccat tgttatttcc
      841 tccaaaaata tgttttaata taaaattatg tagctaggct acttgaagag tgcttatgcc
      901 acttcttcgt ctttttctgc aactgctttg acagcatatg caatgttgcg tactggcgct
      961 tgaagtactg aaaggagcat tgaaagcata ccttctttgt ttggtaattt agcaagagcg
     1021 ttaatttctt caactgaagt gaatttaccg tctactgaac cacctttgat ttcaagagct
     1081 tctgcatctt tagcgaaatc gctaataact ttagctggtg caataacatc ttcgttagaa
     1141 aacgcaactg ctgatggacc aacaaaaagt tctttaaggt cttcaagacc tgctttttca
     1201 gctgcacgag ttaaaattga gtttttgata actttaaact caacatcgct ttcacgaagt
     1261 gaacgacgta agttagtatc ttgctcaaca gtaagaccac gtgaatcaac aacaacgata
     1321 cttgctgctg ctttcatttt ctcagcaata agttcaactt gttcagcttt ttttgcaata
     1381 attgcttcac tcattagttt tacctccgtt tttatttttt gggataggca caaaaaaatc
     1441 gcacctaaac ctagacacga aagtacaata cgttatttta taatgacgtt ttgtgcctcg
     1501 gtaggaatat tatgagcact gctcccctac tgtcttaggt cagtttcatc agaaacttta
     1561 aatagtataa cttataattc tagcattgtc aataattttt attatttttc tgaaataatt
     1621 ttatagtacc aaaaaatact aaaatagtta tctttatatc aaaaaaataa tattttagta
     1681 tttatttatt gacctttact gactaaaggt gtattatagt accagaaaga ta
//
LOCUS       DATRME010000137         1705 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRME010000137 DATRME010000000
VERSION     DATRME010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..943
                     /locus_tag="VBC23_002060"
     CDS             <1..943
                     /locus_tag="VBC23_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsule biosynthesis protein CapK"
                     /protein_id="HEO6843961.1"
                     /translation="RGTQEDITFQRFIAELLKLISTGYALFFYNYYRKADFNSSVVRN
                     VVKVNYFVLFLITVLYLFFPMLKPTLFGRELFSIEWFPHMRIRLAAYFEYATLIGQFI
                     LFSYPILFLKPQKHMENILISLLLTICSYFSGARILLVCMLVLLASLLLDYILFKTNL
                     KLTKKNTFILGMTFLFITACFSYNIWSIIEKIIMYRNQSTITRMIVYQESIIEVLKGN
                     ILFGQGIRIPSSEGIFLGSHSTYISVFYRTSLLGIVLYFSAFILLYKEAISKNYKIYR
                     LFFYTLLCYTLFEEIDPNHWSIVLLFSTFGIVGRAKK"
     gene            940..>1705
                     /locus_tag="VBC23_002061"
     CDS             940..>1705
                     /locus_tag="VBC23_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6843962.1"
                     /translation="MKEKVTVIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGSTD
                     NSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMIEI
                     MLKNLITENADIAEVDFDISNERDYRKKKRRNFYKVFKNNNSLKEFLSGNRVENIVCT
                     KLYKKSIIGNLRFDENLKIGEDLLFNCKLLCQEHRIVVDTTSSLYTYRIVKTSVMNQK
                     FNENSLDFITIFNEISSLVPARLANYVEAKFLREKIK"
BASE COUNT          595 a          206 c          281 g          623 t
ORIGIN      
        1 tagaggtact caagaggata taacgtttca gcgatttatt gctgagctat taaaactaat
       61 tagtacagga tatgctttat ttttttataa ttattataga aaagctgact ttaatagttc
      121 agttgtaagg aatgtggtaa aggttaacta ttttgtgttg tttcttataa cagttttata
      181 tttatttttt cctatgctga agccaacttt atttggaaga gaattgtttt caatagagtg
      241 gtttccacat atgagaataa gacttgcggc atattttgaa tatgctacac taattggtca
      301 gtttatttta ttttcttatc ccatactttt tttgaaaccc caaaaacata tggaaaatat
      361 tttaatatcc ttactgttga ctatatgttc atacttttct ggcgctagaa tactattggt
      421 ctgtatgttg gttttattag cttcgcttct tttagattat atccttttta aaactaattt
      481 gaaattgacc aagaaaaaca cttttatact tggtatgact ttcttattta tcaccgcttg
      541 tttttcttat aacatatggt caataattga aaaaataatt atgtacagaa accaaagtac
      601 tatcactagg atgatagttt atcaagaaag tattattgaa gttctaaaag gaaatatttt
      661 atttggacag ggtataagga ttccatcaag tgaaggaata ttcctaggat cgcattctac
      721 ttatattagt gtcttttaca ggacttcttt attaggaatt gttctttatt tttctgcctt
      781 tatactttta tataaagaag cgatttcaaa aaattataaa atctacagat tattttttta
      841 tacgttatta tgttacacgc tctttgagga aatagatcct aatcattgga gtattgtatt
      901 attattctca acttttggta tagtgggaag ggctaaaaaa tgaaagaaaa agtaacagtc
      961 attataccta tatacaactc agaagcatac cttaaagaat gtgtgcaatc cgtactacaa
     1021 cagactcatc cattgataga agttatacta attgatgatg gatccactga taatagtgga
     1081 gaaatttgtg ataatttatc tcaggaagat aatcgcatac ttgtatttca taaaaaaaat
     1141 ggaggggtct cttcggcaag gaacctaggt ctagataaat ccacaggaga attcataaca
     1201 tttgtggata gtgatgattt tgtagcaccg aatatgattg aaataatgtt aaaaaattta
     1261 atcactgaga atgctgatat agcagaagta gattttgata tttcgaatga gagagattat
     1321 agaaagaaga aaagacgaaa cttttataaa gtttttaaga ataataactc tttgaaagaa
     1381 tttttatcag gtaatagagt ggaaaatatt gtttgtacaa aattatataa aaaaagtata
     1441 attggtaact tgaggtttga tgagaactta aaaattggtg aggatttact ttttaattgc
     1501 aaactcttat gtcaagagca ccgtatagtc gtagatacga cttcttcctt atatacttat
     1561 cgaattgtaa aaacttctgt aatgaatcag aaattcaacg aaaactcatt agattttata
     1621 acaattttta atgaaataag tagtttggtt cctgccagat tagctaatta tgttgaagcg
     1681 aaatttttaa gagaaaagat aaagt
//
LOCUS       DATRME010000138         1609 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRME010000138 DATRME010000000
VERSION     DATRME010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1316
                     /locus_tag="VBC23_002062"
     CDS             <1..1316
                     /locus_tag="VBC23_002062"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6843963.1"
                     /translation="SQQLYKVLNIIKSDYTRRFIVVSAPGKRYEEDLKMTDALIQYYQ
                     NYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLASLPIENNQFLYDCF
                     LAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARIIPGSYDKIENLCLY
                     NEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENFTDVDGIFAAHPGV
                     VKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKNTNNPQQPGTKIVL
                     KHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDLNISFEHMPTGIDD
                     LSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGENMKSQIGVTATAT
                     QALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK"
     gene            complement(1532..1605)
                     /locus_tag="VBC23_002063"
     tRNA            complement(1532..1605)
                     /locus_tag="VBC23_002063"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1569..1571),aa:Asn,seq:gtt)
BASE COUNT          572 a          288 c          274 g          475 t
ORIGIN      
        1 cttcccaaca attatacaaa gtactaaata ttatcaaatc agactacact agacggttta
       61 ttgtcgtttc agctccaggg aaacgctacg aagaagactt aaaaatgaca gatgctttga
      121 tacaatatta tcaaaattat attaacggta aagatattgt taaagaccaa acttggatta
      181 tcaatcgtta tcaagaaatt attagtgatt tgtctttagg aagcactatt gcagaagaaa
      241 ttactcgctc tatagagcag ctagcttcac ttcccataga aaataatcag tttttatatg
      301 attgtttttt agcagccggt gaagataaca acgcaaagtt agttgcaacg ttttttaatc
      361 aaaatgacat tcctgcaaga tatgttcatc caaacgaagc aggaattatt gtaactaaag
      421 aaccatgtaa tgcacgaatt attccaggaa gttatgataa gattgagaac ttatgtctat
      481 acaatgaggt tcttgttatc cctggatttt ttggagtcac agaagataac caaatttgta
      541 ccttttcaag agggggatct gacattactg gatccctaat tgcagcaggc ataaaagcag
      601 acctttatga gaacttcaca gatgttgatg gtatatttgc agcgcatcca ggtgtagtta
      661 agaaccctca cgctatccct gagcttactt ataaagaaat gcgtgaatta gcctatgcgg
      721 gtttttcggt tttacatgat gaagctttac ttcctgccta tcgtggcaga atccctcttg
      781 ttattaaaaa tacaaataat ccccaacagc ctggtacaaa aatagtttta aagcatactc
      841 gtagtaacat agcagtaact gggatcgctt ctgatagccg ttttgctagc ataaacgtat
      901 ctaaatactt aatgaataga gaagtaggtt tcggccgaaa agtactacaa attttagagg
      961 atttaaatat tagttttgaa catatgccaa ctggcataga tgatctatcc attgtcttac
     1021 gtgaaaaaga attgacacca atcaaagaac aagaaatctt aaattaccta actcgtaaac
     1081 tagaagtaga ttacgttgac atccaacaca atttatctac aatcgtaatt gtaggtgaaa
     1141 atatgaaaag tcagattgga gtcactgcaa cagcgacaca agccttatca agagaaaaaa
     1201 tcaatatcac catgatatca caaggttcaa gcgaagtctc cattatgttc gttatagaca
     1261 gtaaggatga aaaaagagct attaaagcac tatatgaaac attcttccaa aaatagtacc
     1321 tattacacta cttacactat tagatagata acaaatcgtc ctaagtaagt ttacttagga
     1381 cgattttatt tagaacatag gatagttttt tcaattttaa tcgtaactac ttggtatcac
     1441 tgacaaattc ggacaattaa gatgctagcc gaccttaagg aataataatt ccaataaaaa
     1501 aaggctaacc aaagttagtc tcctttatct actccgccag taggactcga acctacgaca
     1561 tcatgattaa cagtcatgcg ctactaccaa ctgagctatg gcggattat
//
LOCUS       DATRME010000139         1604 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRME010000139 DATRME010000000
VERSION     DATRME010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(37..651)
                     /locus_tag="VBC23_002064"
     CDS             complement(37..651)
                     /locus_tag="VBC23_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6843964.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
     gene            865..1182
                     /locus_tag="VBC23_002065"
     CDS             865..1182
                     /locus_tag="VBC23_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6843965.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            1179..>1604
                     /locus_tag="VBC23_002066"
     CDS             1179..>1604
                     /locus_tag="VBC23_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6843966.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLK"
BASE COUNT          600 a          256 c          270 g          478 t
ORIGIN      
        1 aaaggctctt ttataattca ttggtaacta ctatttttat tgtaactctt tcaaagatac
       61 aatagcagac aaaccacaat atgacgatgc tcctagtaac aacgacccta aaatatccgt
      121 taaaaaatga ttttctaaat aaagtcttga aaaaataact gatgcaagga ttactaatgc
      181 aaatattacg aaaactactt gtgacaatac tcttctcaat ctctgtcttg caaccactaa
      241 aacaagaatc attaataaaa acgttgatat cgtatgtcca ctgggaaaac tataacctga
      301 gtcaaaaata agttgattca taggtctatg acgtgcgatg atatctttta aaagcaatgc
      361 caaagcattg gtaccaaaaa ataacataaa tagccacaca gctatccttt ggtttaaaaa
      421 atattgatac ccaaggataa caagtgctat taatgacgtt gtcaaagggc tgacaactgt
      481 tgatattatc gtcattaagt tagttaaatc ggagtttcgt atgctttgaa aaaaagctga
      541 agtgatatta tccaaataac ttatcatatc actttgagat ttaactgcta atgttaacac
      601 taaaaaagaa acaagtagta taacacactg gaaaaaggat ttcttgttca ctacataacc
      661 tcactttaat aaagagataa atcttcatac attatggttt aaaatattta aaaaatgctt
      721 aaagcacgcg ctaacctctt aagtttaaat atgactattc acaaactaga agaaatacca
      781 aatacgaact tttcctatta attagcgttc aaattttcat taataaagca atattgctac
      841 aatatacttg aaacggaggg aacaatggat attacagatt atcaaaaatg ggtgagcgaa
      901 ttctataaaa aaagaaactg gtatcaatac aattctttta ttagaagtaa ctttctttca
      961 gaagaagtcg gcgaattggc tcaagccatt cgcaaatatg aaattggacg tgacagacct
     1021 gacgaaaccg aacaaactga tttggaaaac ttaaatgata taaaagaaga actaggagat
     1081 gtcttagata atatcttcat acttgctgat cagtataata tctcactaga agagattatc
     1141 agcgcacatc gcactaaatt agaaaagagg tttgaaacat gagaataact attttttgtg
     1201 gagcaagtac tggggaaaat cctgtttata gtgaaaaaac agttgccttg gcacaatgga
     1261 tggcacaaaa caaacatgct ctggtgtatg gtggaggaaa agttggctta atgggagtca
     1321 tggctgatac tgtgattgca aatggtggct atactactgg tgttattccg acatttttaa
     1381 gagatcgtga aattgcacat gaaaatttat cagagcttat catagtaaat aatatgcctg
     1441 aacgtaaggc aaaaatgatg ctacttggag atgcctttat cgctctacct ggtggacctg
     1501 gtacccttga agaaatttcc gaagttatct cttggtctcg catcggtcaa aatgataatc
     1561 cttgcatttt atataatgtt aatggctact ttaatgattt aaaa
//
LOCUS       DATRME010000140         1595 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRME010000140 DATRME010000000
VERSION     DATRME010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            37..1410
                     /locus_tag="VBC23_002067"
     CDS             37..1410
                     /locus_tag="VBC23_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6843967.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
BASE COUNT          445 a          240 c          322 g          588 t
ORIGIN      
        1 attaaacaca tctaaaaatc ggaaaggtaa tttgctatgt tgaaaaaact atttgcaccc
       61 cctcagaaaa gagaacgttt atcagctgag gaaattaagc ataaatatcc tcgttatcgt
      121 gtacaagtcc ttatttctat ttttgtaggt tatatgggat actattttgt tcgtaatacg
      181 acttcaattt tatctggtat ccttaatatg tctgctactg agattggtat tattacctgt
      241 gctagctaca ttgcttatgg tctctctaaa tttatttctg gtttgatttc agatgaatcg
      301 aattctaaaa ttttccttcc agttggtttg tttcttacag gactagttaa tgtgttaatc
      361 ggtgttattc catctgtaat tactagtatc tggctcttta ctatcatgta tcttattaat
      421 ggttggttac aagggatggg ataccctcct ggagcgcgta cattagtcta ttggtatgat
      481 aataaagaaa gaattaaata tgctaccatt tggaatttat ctcacaattt tggtggagca
      541 atagcgccga ttttaactgg tgtaggtcta gctcttgctg gtaatgattc tcttaatcaa
      601 gcgcgtgctg cttattggtt tccaggggtg gtggcttgtc ttttggctgt tcttgtttac
      661 tttttacaag aagatactcc agaatctatc ggacttccgc ctatagaaga gtatcataaa
      721 gagcaatata ctaatgttgt ggattcaagt gacattttag aagagcctga agttttgggt
      781 atgggcgaaa ttataaaaaa atatattcta ccaaatacta aactgatgtg ggcatccttg
      841 tattcaatat ttgtatatat tttacgttac ggtattgtct catggactcc taagttttta
      901 gcgacgagtg tgcaagatgg tggaaaaggt attactgcaa cagctggtat gggtggtttt
      961 tcactttttg aaataggtgg tattattggg atgctaacag caggatatct atctgccaaa
     1021 gtgtttaaaa actctaaacc tttaaccaat gtagccttct tagttgtagc aatcttatta
     1081 ctagctgcct attggtttat tccagcagga cctcaatata tggcattaga cttcattatt
     1141 ttactaggtt tgggggcatc aatctacggt cctgttatga tggttggcct gtatgctatg
     1201 gagttagttc caaaagcagc agctggagca gcttcaggat tgactggcac atttagttat
     1261 gtcggaggag caacgatcgc tactctagct attggtatta ttattgacca ctttggttgg
     1321 ggagtagcat ttataatatt tggtatttct ggatttgcgg ctattgtctg taccttgctt
     1381 tcaagagata agagtctaga gtattggtga tattatttgg taaaagtatc atttaatatt
     1441 tagtgagata agttatgaaa aaaggcctct tgaaagagac cttttttgtt ttagataatc
     1501 tatttgttga attaattagt gatatcgatt gtttttaata gatagtttct acctagttaa
     1561 aataaaacta ccccattaat aatgattcaa tttta
//
LOCUS       DATRME010000141         1521 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRME010000141 DATRME010000000
VERSION     DATRME010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1410)
                     /gene="gtfA"
                     /locus_tag="VBC23_002068"
     CDS             complement(<1..1410)
                     /gene="gtfA"
                     /locus_tag="VBC23_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6843968.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDI"
BASE COUNT          498 a          294 c          192 g          537 t
ORIGIN      
        1 aatatcttta gaattaaata aagcaacaat atactgagca aaagcagttg atatttgttg
       61 aggattatct ggtgtttccc ttggaataag gtagccattc aaattatttt caataaaggt
      121 ctgattacca tacggaacat ctaaaccaat tatcggaagc cccgatccga ttgcctccat
      181 caaggttaaa ccaaaacctt cactggtaga acccgataaa taaacttgat aatctttata
      241 aacagaagat aggtttttat gccccattaa tcgtatataa ttatttgcct tatttttagc
      301 aattatttct tgtaactttt gccgttcgcc accttcacca taaatatcaa aggttaattc
      361 aggaattact ttattagctt ctaccacagc attaattaac caatcaatat gtttttccga
      421 agctagacgt gagcaagtta acaacgaaaa agcttttctt tcatgccact cttttctctg
      481 taattttgat aaacttccta caggaattgt ataaacgacc ggttcaatat ttaaatactt
      541 tttaaattga tcaatcaacg tcttagtttg cgtttctgtt gaggtgataa aagcatctat
      601 gctatcagca tttgaaaatt gatagtcata ataattattc cataaaatcg tagtttcatc
      661 agttgcgcta acattataat gctctgcatg aacaacaaca gctactttag catttccttt
      721 attccttaga acagcttgcc caatgcctgt agagcggtct aacaagatta gatcactatt
      781 cgttaattgt aatttttcta acatgtatcc aactaattct tctttagaat atagtatagt
      841 ttctttgaat ttaaaaatac tattctcgcc atctacattt tcttcatatg caacacttcc
      901 atcttcatta taaaatgtac gctgatacag gcatggacct atatcttcgg gagcataata
      961 ttctgaaaac attttagtat atgagtagta gtcttttcta acaagctttc cccccataag
     1021 atactctacc ctttggacga tgttactaga ttcatcgtta agcataactg taactacaat
     1081 actacttcct ttaaaaaaaa atgatattat tttaccgttt ttctcagtcc tatcaatcgg
     1141 tagtgaaaaa ctttctttca attgttgcag tgaataagaa gttgcagcaa ttgttaaatc
     1201 agtaaaaaaa gtataaagcc atataatctc attgtcttca aagccgatat ttcgagtaag
     1261 gtgctctata ttttcattct gaaacatatc agtaaaaata aatttagctt caacacctaa
     1321 ctttctaaaa acttctgaac gatatgcttg agcatactct acaccgctac ttgcccaacc
     1381 tatccctcta ttcaaattat aaactaccat taactccgtt ttcctttata cataatatat
     1441 tgagtaactt ccatcacttg cttttgaaat tgtacttaac ataatattct taacgataga
     1501 atatcttatt tcttttccca t
//
LOCUS       DATRME010000142         1334 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRME010000142 DATRME010000000
VERSION     DATRME010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..1227
                     /locus_tag="VBC23_002069"
     CDS             <1..1227
                     /locus_tag="VBC23_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6843969.1"
                     /translation="IPYLILSILGLIVIYSTTSATLIQLGANPFRSVINQGVFWAVSL
                     VAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGAHGWIVIGPISFQPA
                     EYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLKDWRFYSLFMIGLVI
                     AQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFIGTIAVVGVETMAKV
                     PVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGLGNSIEKLGYLPEAT
                     TDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNSMIALGIGAMLLMQV
                     FVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDANEKKELIMKEAEEQY
                     KPQEKNEKIINLDAFK"
BASE COUNT          398 a          193 c          266 g          477 t
ORIGIN      
        1 attccctatt tgatattgtc tattctaggc ttaattgtaa tttattcgac aactagtgca
       61 actctaattc agcttggagc taaccctttt cgttcagtaa tcaaccaggg tgtcttttgg
      121 gcagtcagtt tggttgcaat tatctttatc tataaactga aactaaattt tttgaaaaat
      181 tctaaagtgt tgactatggc agtattggta gaagtatttt tgctactaat agcgcgcttt
      241 ttcacacaag aagttaatgg agcacatggt tggattgtta ttggtcctat tagttttcaa
      301 cctgctgaat atttaaaagt tatcattgta tggtatttag cgtttacttt tgctcgaagg
      361 caaaaaaaga tagaaatata tgattatcaa gctttgacaa aagggagatg gcttccgaga
      421 tcactaagcg acctaaaaga ttggcgattt tattctttat ttatgattgg tttggtaatt
      481 gcacaacctg atttaggaaa tggttcgata attgttctaa cggtcattat tatgtactgt
      541 attagtggta ttggttatcg atggttttca gctttacttg gtctaatcgt cgtggggtca
      601 actttattta tcggaactat tgcagttgtt ggagttgaga caatggctaa agtccctgtc
      661 tttggttatg tagctaagcg atttaatgct ttttttgacc catttaaaga tttaacagat
      721 tcaggacacc agttggctaa ttcatattat gcaatgagca acggtggctg gtttggtcga
      781 ggacttggga attctattga aaaattaggg tacttaccag aagcaacaac agactttgtc
      841 ttttcaattg ttattgagga attaggagtt attggtgcag ggtttatttt agctttggtt
      901 ttcttcttaa ttttacgtat tatgcacgta ggtataaagg ctaaagatcc tttcaactca
      961 atgatcgctc ttggtattgg agcaatgcta ttgatgcaag tttttgttaa cattggtggt
     1021 atttctgggt taatcccctc aactggagtg acatttcctt tcttatccca aggagggaac
     1081 agcctcttgg tactttctgt agctatcgga ttcgtgctta atattgatgc taacgagaaa
     1141 aaggaactca taatgaaaga agcagaagag caatataagc cacaagaaaa aaatgaaaaa
     1201 ataatcaacc ttgatgcctt taaataatca tccaaacaaa aaacgagatt tttatctcga
     1261 tttttgtatt ttttgaaatt taaactctaa ttaatagaga gaagatattt gaagaaaaga
     1321 agtgtctatt aata
//
LOCUS       DATRME010000143         1329 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRME010000143 DATRME010000000
VERSION     DATRME010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            304..1227
                     /locus_tag="VBC23_002070"
     CDS             304..1227
                     /locus_tag="VBC23_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6843970.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          454 a          215 c          216 g          444 t
ORIGIN      
        1 aagaaataat gagactacac ttaacaaaat aaaaatcacc aaaagaaata aattaatcac
       61 ccgtagaggt gtatgtgagt gtttcttttg gtgacggcgc gaatgattag acattgtaat
      121 tctccaatac ttaaaaaaac cttaatttta cttggatatt atatcatttt tcaatacaat
      181 attcaacgaa aataatattg gaataatctg attatattag ctaaataaca ttttcaattg
      241 atctatattg ataatttagc tgatctaatt gaatttattt acattctagt ttaaactaga
      301 attatgagaa ttcaacaatt acaatatgtt attaagattg tagaaactgg tagcatgaat
      361 gaagccgcta aacaactcta cattacccaa ccttcacttt caaacgctgt taggaacctt
      421 gagactgaga tgggtatcca aatttttatt cgaaatccaa aaggaataac gttaacaaaa
      481 gatggtatgg aatttctgtc atatgcgcga caaattctag aacaaacagc acttcttgaa
      541 gagcgttata agggtgataa tacaagtcgt gagctgttca gcgtttcttc acaacactat
      601 gcatttgtgg ttaacgcttt tgtagctctc tttaatggca cagacatgac ccaatacgaa
      661 ctttttttac gtgaaactag gacttgggaa attattgatg atgttaaaaa tttccgttca
      721 gagattggtg ttctgtttct taatagctat aatagagatg tattaaccaa attattcgac
      781 gataattcct taattgccac aactcttttt actactacac ctcatatttt cgtgagcaaa
      841 tccaatccct tagctaatag aaaaaaatta agtatgaaag atttagaaga ttatccttat
      901 ctgagttatg atcaaggcct gcataattct ttctactttt ctgaggaaat gatgtctcaa
      961 attcctcacc caaaatccat tgttgtcagt gatagagcaa ctctgtttaa tttaatgatt
     1021 ggattagatg gttatacagt ggcaactggt atcctcaact caaaattgaa cggtgacgaa
     1081 attgtcgcaa tccctcttga cgtggatgat gttattgata ttgtctatat ccgacacgac
     1141 aaagcaaacc tttcaaaaat gggacaaaaa tttattgact atttattaga ggaagtttcg
     1201 tttaatcata aaaataaaca gtcctgagat tgttgtactg acccccaaaa gttggacaat
     1261 ttattttaag tgaaggattt agttctgtat tgcacaggac taagtccttt tagttttgct
     1321 ttgattcgc
//
LOCUS       DATRME010000144         1298 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRME010000144 DATRME010000000
VERSION     DATRME010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            40..372
                     /locus_tag="VBC23_002071"
     CDS             40..372
                     /locus_tag="VBC23_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843971.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKKE"
     gene            374..883
                     /locus_tag="VBC23_002072"
     CDS             374..883
                     /locus_tag="VBC23_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843972.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
     gene            1054..>1298
                     /locus_tag="VBC23_002073"
     CDS             1054..>1298
                     /locus_tag="VBC23_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO6843973.1"
                     /translation="MKKKYWILLILLLTVLSGGGYMVFKQHTEKEKMIAIAHSAEAMQ
                     IYEKHMKANDSRAFSEQGIIHNYKINDSELNYNPMGG"
BASE COUNT          520 a          148 c          249 g          381 t
ORIGIN      
        1 ttgataataa taatcacagt aattttagga ggactagcca tgaaaaactt atttgcaaca
       61 acagaagcat catcaaggaa acaggaacaa gatagaattg tcaattacat aaaacaacat
      121 gttgagttaa caaatggtaa tcaaataaaa aaaattgagt ttatcgactt tcaaaaaaat
      181 gagatgacag gtacatgggg aatttctact aaaattaatg aacaattttc gattagtttt
      241 tctgaagata gaattggtgg taaacttaga gcattaggat atcaaccgaa tgaaataggt
      301 ttttcaaagg acatcaatag taataatcaa aatgttaatg atattgaagt gatttatatg
      361 aagaaagaat agaatgattt cagaagaaag ttacagatat ttagttgagg atgcttatcg
      421 agttgattca aaaaaagtaa aaatacctct gaaaagaagt gatattgtag ggaacagtga
      481 ctatgttatt attgaaccac ctatagataa tacttcaaat ggtatgcaag ctatggtagt
      541 tgctccgata aaagaaggaa tgacagctga accagatact agtgaaatag tgattgttta
      601 tgcaggtact aatttaggtg agcgtttaga catagctaca gatgttgaaa tggttgctgg
      661 aggggataca tatttacttg ctgatcctaa aaccaagact tttaggaaat ctcagggaaa
      721 atcagcacta gagtacgcag aaaaaatatc gagtaaatat cctaattcag aaattactac
      781 aaccggtcac tccttagggg aatcagaggc actatatgtc gctttaaaaa tgggttggat
      841 gaatgttggt tataatggtc cagatattca taacatgata taaaatgacg aaatagatta
      901 tatgaaatct catccagaat aatttagaaa aaaatcgaaa aatatgagtt gtttgagtta
      961 aaattttaac tatggagata gagtcctgta ttatttgatt ttgtagtaaa ctagttagga
     1021 atttattcat aggatattat aaaggagtta atgatgaaga aaaaatattg gatattacta
     1081 atattactat taacagtttt atcaggaggc ggatatatgg tttttaaaca acatacagaa
     1141 aaagaaaaaa tgattgcaat tgcacacagt gcagaagcga tgcaaattta tgagaagcat
     1201 atgaaagcaa atgattcgcg agctttttca gagcaaggaa ttattcataa ttataaaatt
     1261 aacgattcag aactcaatta taatcctatg ggaggaat
//
LOCUS       DATRME010000145         1283 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRME010000145 DATRME010000000
VERSION     DATRME010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            27..1235
                     /locus_tag="VBC23_002074"
                     /pseudo
     CDS             27..1235
                     /locus_tag="VBC23_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766719.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          390 a          179 c          253 g          461 t
ORIGIN      
        1 taccagccta taatgaatca acaaccattg tgagttcgat tgattcgtta ttgcaccttg
       61 attatgaagc gtatgaaatt atcgttgtgg atgacggttc cagtgacaat acttcagatg
      121 ttctcaagga agagtttgca ttaatgaaaa tctcgaatac gattgattca attattgcta
      181 ctcaaacttg taaagatgtc tttcagagac aggttggtaa ggttaaactg acactcattg
      241 ttaaagaaaa tggtggcaaa ggggatgcct taaatatggg gatcaatgct gctaattacg
      301 attattttct atgtctagat gcagattcta tgttgcaggt agattctttg agtcaaatta
      361 gcaaatccat acaggtagat ccgacggtta tagcagtggg agggcttgtc caagttgccc
      421 aaggtgttaa gattgagcag gggaaagtag catcttatcg tttgccttgg agaattattc
      481 cttgtgcaca agcacttgag tatgattcat catttttagg agctcgaatc tttttagact
      541 atttaagagc taatttaatt atttcggggg ctttcggtct ttttaagaaa gaccttgtta
      601 aagcggtggg aggatatgac acccagactc taggagaaga tatggagttg gtgatgaaat
      661 tacatttctt ctgtcgtaat aataatattc cttatcgtat ttgttatgaa acagatgctg
      721 tttgttggag tcaagcacca acgaatttag gggatttacg taaacagaga cgccgatggt
      781 atcttggctt gtactaatgt ttaaaaaaat ataaaagtat ttttgccaat tatcgctttg
      841 gagctgtagg atcaatttct tatatttact atattttatt tgaattactg acccctttta
      901 tagaatgttt tggtattgtc attatatttt tgtccttatt atttaatcaa ttaaacatcc
      961 cattctttat atcattggtg agcctttata tcttttactg tgttttaatt acattaagct
     1021 ctttcttaca ccgtatttat tctcagcaac tagtaatagg aattttagat attgttaaag
     1081 tattttatat tgctgttttt aggtatctta tcctacatcc tgtattaact tttgttaagg
     1141 ttgcaagtgt tattggctat aaaaataaaa aaatggtttg ggggcatatt acgagagaga
     1201 aacaaatgaa ggattttgaa acaagtgtta cttagaagtt atagcgattc atatatataa
     1261 aataatcact aaaagagcgt aat
//
LOCUS       DATRME010000146         1236 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRME010000146 DATRME010000000
VERSION     DATRME010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(1..471)
                     /locus_tag="VBC23_002075"
                     /pseudo
     CDS             complement(1..471)
                     /locus_tag="VBC23_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            525..866
                     /locus_tag="VBC23_002076"
     CDS             525..866
                     /locus_tag="VBC23_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEO6843974.1"
                     /translation="MSTFIVYIHLSERLEEFKTEAFDFKTRASYVTAKLFFLGNMIKH
                     NTNSSKELIRSLKNDKSVLAMIPHKELFNRSTLDKKSLSYMMSDKELYSRDSKFFKEI
                     DFTFRKITDKL"
     gene            935..1165
                     /locus_tag="VBC23_002077"
     CDS             935..1165
                     /locus_tag="VBC23_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843975.1"
                     /translation="MAFEPKNSELTKVLKESLDEEKKEIFSSEMNIRDFERTKQYQFT
                     LQPSVRKKIDRLSKEKGYRSASSFINDFFKNL"
BASE COUNT          440 a          168 c          204 g          424 t
ORIGIN      
        1 tagacttcac accaagtctt ttgttcgctt tggtataaat ctcaagtatt tgttttctat
       61 aaatttgatt atccaattct ctttttgagg gcttatgttt tagaaattta tagtaagtgg
      121 agcgattgac acgtaaaaca cgacacaagg ttgttgtggc gtgttcaaag cgtaaccgat
      181 agacagcgag gagtcttact ttaagttttg catgaatatg gcacttgctt tttttaggat
      241 aaggttctcc tcttcaagct gcgcattacg tttttgtaac tcttgtattt gcttggcagt
      301 aagtacagaa ttatcttcga gtttgacttg ggaatactgt ttgatccatt ttgcaagcgt
      361 agaagtggat accccatagt ctttacagag ttcagactga gtttttccag tttgataaag
      421 gttaacaaga gattgtttga attcttcatc atagcgttta aatgttgaca taggttgtcc
      481 tttcgttttt gtgtctttta aaacagattg taacacacaa agttttgtct acttttatag
      541 tatacatcca tctttctgaa agacttgaag aatttaaaac agaggcattt gactttaaaa
      601 ctcgagcaag ttatgtcaca gcaaaattat tctttttagg taatatgata aagcataata
      661 ccaactcttc aaaagaatta ataagatcac taaagaatga taaatcagtt cttgcaatga
      721 ttcctcataa agagttattt aaccgctcaa cattggacaa aaagtctcta tcttatatga
      781 tgtctgataa agaattatat tcaagagata gtaaattctt caaagaaatt gattttacat
      841 ttaggaaaat tactgataaa ctataaatat aattacaaat ataaacatag ttatatacat
      901 aaatatgact atgtttataa gaaagaggat gaatatggca tttgaaccaa aaaattcgga
      961 actaacaaaa gttctaaaag aaagtttaga tgaagaaaag aaagaaattt tctcatctga
     1021 aatgaatatt agggactttg aacgtacaaa acaatatcaa tttacacttc aaccttcagt
     1081 ccgaaaaaaa attgataggc tatcaaaaga aaaaggctat cgttcagcat catcttttat
     1141 aaatgacttt tttaaaaatt tgtaaacaaa gtttgaatta tatttattat ggaaatcatt
     1201 attagatggg aaaaatggtt aagaaaaaag tggttg
//
LOCUS       DATRME010000147         1226 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRME010000147 DATRME010000000
VERSION     DATRME010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            98..>1226
                     /locus_tag="VBC23_002078"
     rRNA            98..>1226
                     /locus_tag="VBC23_002078"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          335 a          249 c          372 g          270 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaacaataa
       61 atctgtcagc gagacagaaa agagcaaagc tcaaactttt aatgagagtt tgatcctggc
      121 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgag gtttggtgtt
      181 tacactagac tgatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctcatagcg
      241 ggggataact attggaaacg atagctaata ccgcataaga gtaattaaca catgttagtt
      301 atttaaaagg agcaattgct tcactgtgag atggacctgc gttgtattag ctagttggtg
      361 aggtaaaggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
      421 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
      481 acggaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct
      541 gttgttagag aagaacgttg gtaggagtgg aaaatctacc aagtgacggt aactaaccag
      601 aaagggacgg ctaactacgt gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc
      661 ggatttattg ggcgtaaagc gagcgcaggc ggttctttaa gtctgaagtt aaaggcagtg
      721 gcttaaccat tgtacgcttt ggaaactgga ggacttgagt gcagaagggg agagtggaat
      781 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accggtggcg aaagcggctc
      841 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc
      901 tggtagtcca cgccgtaaac gatgagtgct aggtgttagg ccctttccgg ggcttagtgc
      961 cgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag
     1021 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag
     1081 aaccttacca ggtcttgaca tccttctgac cggcctagag ataggctttc tcttcggagc
     1141 agaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     1201 ccgcaacgag cgcaacccct attgtt
//
LOCUS       DATRME010000148         1224 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRME010000148 DATRME010000000
VERSION     DATRME010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            30..>1224
                     /locus_tag="VBC23_002079"
     CDS             30..>1224
                     /locus_tag="VBC23_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO6843976.1"
                     /translation="MTYQKTVVLAGDYSYIRQIETTLKSLCVYHENLSIFIFNQDIPQ
                     EWFLAMKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQYISADTV
                     LYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWRQENMTI
                     KLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHKFIFDIP
                     ITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHPIKTQNY
                     RLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLHKTYTPP
                     IIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPETVFASIDDLI
                     NYLQSI"
BASE COUNT          416 a          213 c          179 g          416 t
ORIGIN      
        1 atctaactaa tataaataaa ggatttttta tgacttatca aaaaacagtt gttttggctg
       61 gtgattattc ctacattaga caaattgaaa ccacattaaa atctctctgt gtctatcatg
      121 agaatctctc aatttttatt tttaatcaag atattcctca agaatggttt ttagctatga
      181 aagatagggt tggacaaact ggaaatcaaa ttcaggatgt aaagctcttc catgatcact
      241 tatccccaaa atgggaaaat aaaaagctta atcatattaa ttatatgacc tatgctcgtt
      301 atttcatacc tcagtacatc tcagctgata cagttttata tcttgactct gacttagttg
      361 ttactactaa tttagataac ctctttcaaa tttcactaga caatgcatat ttagctgcag
      421 ttccagctct ttttgggctt ggatatgggt ttaatgctgg agtaatggta attaacaacc
      481 aacgttggcg acaagaaaat atgactatta aattaattga aaaaaatcaa aaggaaattg
      541 agaatgccaa cgaaggggat caaacaattc ttaatcgcat gtttgaaaat caggtaattt
      601 atttagatga tacctacaat tttcaaattg gttttgatat gggagctgct atcgatgggc
      661 ataaatttat ttttgacatc ccaattaccc cactcccaaa aattattcac tacatttcgg
      721 gaatcaaacc ttggcaaaca ttatcaaata tgagactccg tgaggtatgg tggcactata
      781 atttacttga atggtcaagt atcatatcta gtaaaaaagt atttggttta gaccacccaa
      841 ttaaaacaca aaattatcgt ctcaatttcc ttattgttac aacttctgat tgtataccat
      901 ctatctcaga attagtcact gcccttccag attgtctatt tcacattgca gcaccaacag
      961 ttatgtctga cattcttata agcctattag cttttgaaaa tatccgcctt cataaaactt
     1021 acactccacc tattattgat aaattattag cagaagctga tatttatctt gatattaatg
     1081 tttttggaaa aatggagggc attctacaga aaatgtcaca atctgaaaaa ccaattctta
     1141 ctacaagaga atgcaatagc cagcttgttc cagaaacagt ttttgcatct atcgatgatt
     1201 taattaatta tcttcagtct atat
//
LOCUS       DATRME010000149         1220 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRME010000149 DATRME010000000
VERSION     DATRME010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            294..>1220
                     /locus_tag="VBC23_002080"
     CDS             294..>1220
                     /locus_tag="VBC23_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO6843977.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MTLHNDVKDIMYGCKCIYDAGKQEKLNLPLFYH"
BASE COUNT          493 a          142 c          236 g          349 t
ORIGIN      
        1 gaatgatgat attatctttc agttagctga aatctcagaa tttttaaagt caagtcagtc
       61 aacatttaaa caggagcgta gtccatattc tcaaataata gacctacgaa aagaagaagc
      121 acaagctaaa agtgttagga atagcataga aaaattattg taagaaaaca gtgatctaat
      181 acaggtaagg aagtatgagt taggctataa aactaagtaa aaaaatatga tataatataa
      241 aaaattatcg agtaaaataa actggaagcg aaacttaagg agatgattat taaatgaaaa
      301 tatgtatttt aggattaggc gtaataggca caacttatgg atatatcttt caaaaagcag
      361 gacatcaggt tgaacattta gttagagaaa gtaaaaagca agatgttcca ttaaagctgg
      421 atataaccat gttagatggg cgatataaca ataagggaga agaaaaaaca gatatatata
      481 ctgtgaatct tgcaaaacca aatacaaatt atgactttat ccttttaagt gttgcaggcg
      541 ggaggataaa agatgccata gcaacacttt cacaaaataa tattacagga agtttgttat
      601 tattttgtaa cttttggaac agccgtgaag aaatcgaaaa aatggttggt gattttaaat
      661 atatcattgg atttccgaca gcaggtggga atttgaagca gaaaaagttg aattgtgttc
      721 tgtttgacca tataatgctt gaaagtgaag aaaaagcaac tatcacaaac tataaagagt
      781 tgttagaatt tctcgaatct gccgacataa aagtagaaaa tccttatgat atgatagagt
      841 ggatatggat tcatatggca ataaatgcag gtgttacttc tactgcagca agggcaggaa
      901 aaattgatga tccaaaacaa cttgcggtta atcttatgag agataatcat ggattgtcta
      961 tcgcggtaaa atcaataaga gaaacaatta aagtagtaaa aaatagaggg gtcgatttaa
     1021 gattatataa aaatgaactt tttccttata aaataccttc tactattgca ggaataatta
     1081 tgaaacgaat gttcgcaaga aatgaactta cgagtagaat tatgacgttg cacaatgatg
     1141 taaaagatat tatgtatggt tgtaaatgta tctatgatgc tggaaaacag gaaaaattga
     1201 acttaccatt gttttatcat
//
LOCUS       DATRME010000150         1199 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRME010000150 DATRME010000000
VERSION     DATRME010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(1..409)
                     /locus_tag="VBC23_002081"
                     /pseudo
     CDS             complement(1..409)
                     /locus_tag="VBC23_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            587..787
                     /locus_tag="VBC23_002082"
     CDS             587..787
                     /locus_tag="VBC23_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6843978.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            829..1017
                     /locus_tag="VBC23_002083"
     CDS             829..1017
                     /locus_tag="VBC23_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6843979.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          421 a          160 c          236 g          382 t
ORIGIN      
        1 tgttgtcatc aattattttt ctaaattccc tggccttaaa ttgtgaccct tggtcggaat
       61 gaaagagaag tgttccttca atctttcttt tattaagagc taattctaga gtgtcacatg
      121 ctaactttgc atccatacga tgactcaatt tccaagcaat atattttcta gaatagagat
      181 caattattgc gcagagatag acatatttct tatatccaat agaaatatag gtgaagtcag
      241 tagaccatac ttgattaggt ttatcaggat aaaatttctg gttgagtaag ttttgaggat
      301 acgttttttg agtcttttta agagctgttt ttgtcttaac ggtagccatt ttagggagcg
      361 ccatattctt catcagacgg taaattcttc cttcagagat ttttgtgtct tttaaaacag
      421 gttataacac acaaagtgtt gtctattttt atagtataca tccatactcg cataaccatg
      481 agataatcac taaaacaaga gtcgcctgac actagcgact cttgttttta tttgctaaag
      541 aaagatatag atgttatgat taataaaaat aataaggagg tgcagtatgt cacaagaaaa
      601 actaaaatca aaattagatc aagcaaaagg tggtgctaaa gaaggctttg gcaaaataac
      661 cggtgataaa gagttagaag caaaaggatt tatcgaaaaa acaattgcta aaggcaaaga
      721 actagcagat gatgctaaag atgctgttga aggggcagta gatgctgtca aagaaaaact
      781 gaagtaacaa ataaccgctc atttaacgag cggttatttg ttatcttttt gtctatcaga
      841 acagatatag atgagtttaa agaagcgatt gataaaggct atatttcagg gaacacagta
      901 gcgatagtgc gtaaaaacgg aaagatattt gattatgtgt tactacacga agaagtgaga
      961 gaagaagagg ttgttacagt tgagagagtg cttgatgtac tgaggaagtt atcataataa
     1021 cggaccaaaa ttcgccccaa aaagtatttt aaaatagaat aaaataaaaa aatatgcttt
     1081 ttattatgga tgtatcgtgt attttaacct ttaatatatc ctattttaat ggtagagaag
     1141 ttggcagggg acatattaaa accttttttg agatgttagg aaggatttta ttctttaga
//
LOCUS       DATRME010000151         1104 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRME010000151 DATRME010000000
VERSION     DATRME010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..285
                     /locus_tag="VBC23_002084"
     CDS             <1..285
                     /locus_tag="VBC23_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6843980.1"
                     /translation="SQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQ
                     INRKHYQNIKQVQLDCFEYIENFYNNYIPHTANLGLTPNQKEENYFNAIK"
     gene            complement(406..738)
                     /locus_tag="VBC23_002085"
     CDS             complement(406..738)
                     /locus_tag="VBC23_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6843981.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
     gene            921..>1104
                     /locus_tag="VBC23_002086"
     CDS             921..>1104
                     /locus_tag="VBC23_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6843982.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRSFY
                     NHYNSKEDFLRESILII"
BASE COUNT          374 a          188 c          163 g          379 t
ORIGIN      
        1 tcacaattta aggccaggga atttagaaaa ataattgatg acaacaatat catgcattct
       61 ttttctaaac ctggatatcc ttatgataat gccgtaactg aagcattttt caagtattta
      121 aagcatagac aaatcaaccg aaaacattat caaaatatca aacaggttca attagactgc
      181 tttgaataca ttgagaattt ttataacaat tacatcccac atacggctaa tctaggacta
      241 acccctaatc agaaagaaga aaattatttt aacgcaataa aataacacgg ttttctgtct
      301 acttatttga cattagtcca ctctgtgaat tcagatgtgg aagaagaagt aactcgcaat
      361 gttttatttg cttctttttc gtgtgtcact ggaaccgctg catcttcagc attctaatcc
      421 tacagcatgc tccgctgatt ctgtaggatt agaatgagat gcatctgttg ataacactgc
      481 tgaagttgac gtatctgtcg caagacttgt tacatctgaa gtaggctgtg taacagtaac
      541 ttccggtttt acaccagctg tttgtggtac ctcagtactc ataatgcttg actcatccgc
      601 caaaacagaa tgactactca ttcctagaaa tgtcaatcca attaataccg atgctacacc
      661 aaatttgaat ttcttaatcg aaaaacgctg tttcgtatgt gcgacattat aatttctttg
      721 cttatacttc ctaagcataa cttctctcct tttcaaaagt aaaaatagat taaccaataa
      781 agttatctat ttttactttt tttaaagttt tactgtttta cattatctcg ataaatgtga
      841 actattattt gaatttgtat tgtataatca cattcgataa gatttataac tttaagattt
      901 tatataaagg tgatagtata ttggtaagtt cagataacac taatttaaaa tttttaaaag
      961 ctttctcaga attgttaaag atgaggagct ttgagcaaat taaagttagc gatttagcaa
     1021 aaaaagctcg acttagtaga agatctttct ataatcatta caattcaaaa gaagacttcc
     1081 ttagggagtc tattttaata atct
//
LOCUS       DATRME010000152         1083 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRME010000152 DATRME010000000
VERSION     DATRME010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(15..866)
                     /locus_tag="VBC23_002087"
     CDS             complement(15..866)
                     /locus_tag="VBC23_002087"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO6843983.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPICAGLGGG
                     STDAAATIRALNRLWNLQMGHDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
     gene            complement(1006..1079)
                     /locus_tag="VBC23_002088"
     tRNA            complement(1006..1079)
                     /locus_tag="VBC23_002088"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Asn,seq:gtt)
BASE COUNT          386 a          232 c          133 g          332 t
ORIGIN      
        1 gaagataaaa ccttttatct caacaaccta actttataaa cttctttgca aaaacctttc
       61 atactattaa aaacacgatc agcttttttc tctgtagaac acattgaaaa aacagttggt
      121 ccacttcctg tcattaatgc aacatcggct ccagaattta acatacgttc ttttattgta
      181 cttataactg gatttttagt aattgtaata tcctcgagtg aatttcccat agatttgacc
      241 attaactgat aatctgatga caaaatagca gactttaata aatcaatatc aactctgctt
      301 atagacttac aatcaatatc tctaaaaatg gatttagttg aaataccaaa atccggctta
      361 accagaacta tccaacatgg tctcaatgtc ggtaagggtt taacaatttc acctttacct
      421 aatactaacg aacatccccc accaaggcaa taaggaacat cactaccaat tttaaaacca
      481 atagcaacca tttcgtcatg gcccatttga agattccata atcgattaag agctcttatt
      541 gtagcagcag catcagtaga cccccccccc agtcctgcac agataggaat ggatttttct
      601 aatctaatat gaacaccttt attaatacca tattgatttt tgattatatc tgcagcttta
      661 aacacatcat tatcattatt taaaggcatt ttgctactat cagaatcgat aacaatacaa
      721 tcttccttta gctcagaaat ggtaacgtag tcattaagat caatactaac cataatcata
      781 gctaattcat gataaccatc gtcacatcgt cctttaatat ctaatcctaa attaagtttg
      841 gcaggagctt tctcaaaaat tttcataaaa cctccctaat aaaatataga atatccatat
      901 tataacataa caaatgacaa attcggacaa ttaagatgct agccgacctt aaggaataat
      961 aattccaata aaaaaaggct aaccaaagtt agtctccttt atctactccg ccagtaggac
     1021 tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc tatggcggat
     1081 tat
//
LOCUS       DATRME010000153         1078 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRME010000153 DATRME010000000
VERSION     DATRME010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            4..>1078
                     /locus_tag="VBC23_002089"
     CDS             4..>1078
                     /locus_tag="VBC23_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6843984.1"
                     /translation="MIILVILSMATLSIISLSIKDATDRASKETFANITNSFSMEINR
                     QVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLVDHDIIETQDTLANQSPE
                     RAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDKNKILMHKDLAKKNNLKV
                     GDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSAAQELYENTLITDVHSAA
                     KVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSI
                     SGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFIIEM
                     VFISIPALLGSYFLAQYTADKLGN"
BASE COUNT          433 a          138 c          185 g          322 t
ORIGIN      
        1 ttaattatta tattagttat tctatcaatg gcaactttaa gcatcattag tttatctatt
       61 aaagatgcca cagatagagc ttctaaggaa acatttgcta atataacaaa cagtttttct
      121 atggaaataa atagacaagt aaatccagga acacctaggg gtggaggaaa tgtaaaaggt
      181 gaagatatta aaaagatatc tcaaacaaac agtatagact cctatgtaaa aagaataaac
      241 agcgtcgcag acctagttga tcatgatata attgaaactc aagatacact tgcaaatcaa
      301 tcacctgaaa gagcaaagaa ttttaagaga acagttatgt taacaggggt taatgactca
      361 gcaaaagaaa caaaatttgt atcagaagca tataagttag tagaaggaaa gcatttagaa
      421 aataaagata aaaataaaat tttaatgcat aaagatttgg caaaaaaaaa caatctcaaa
      481 gttggagata aaataaaaat aaaatctaat ttgtttgatg ctgacaatga aaaagttgca
      541 aatgaaactg tagaagtaga aattaaaggt ttatttgatg gtcataatag cggtggggta
      601 agtgcagcac aagaactata cgaaaataca ctaatcactg acgttcattc agctgctaaa
      661 gtttatggta atacagaaga tacagccgta taccaagatg caacattctt tgtaaaaggt
      721 gataagaatc ttgatagtgt aataaaagat cttgggaaac tagatataaa ttggagagaa
      781 tataatttga ttaaaagttc atcaaattat cctgcgctgc aacaatctat atccggtatc
      841 tattctattt caaacaaatt atttgttggc tcattaatat ttgcaggagt tgtagtttca
      901 ttattacttt tcttatggat gaatgccaga aagaaagaaa tagcagtatt actatcatta
      961 ggtatatcta aattagaaat ttttggtcaa tttataatcg agatggtatt tatatccatt
     1021 cccgcacttc ttggatctta tttcttagct caatatacag ctgataagct agggaata
//
LOCUS       DATRME010000154         1037 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRME010000154 DATRME010000000
VERSION     DATRME010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            64..1029
                     /locus_tag="VBC23_002090"
     CDS             64..1029
                     /locus_tag="VBC23_002090"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6843985.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
BASE COUNT          376 a          173 c          179 g          309 t
ORIGIN      
        1 gacatatctt ttgttaaact ttcttcgaaa acgttttcga atataagtat cggaggttgt
       61 aatatgacaa ttaaaaatct cactgttgca ggaagtggcg ttctaggtag tcaaatcgcc
      121 tttcaagcag cttacaaagg aatgtctgtc accatatatg acattaacga tgaagcttta
      181 aataagggaa aagaacgcat caaaaaatta gctaaagtct atcaatcaga aattgaaaca
      241 gcaaaagaag catatagtga taaagctaaa agtatcaagt ataataaaaa cttattgcct
      301 agcttagacc atatttttct ttcaaaagtt gccgattcat tagacttgat tgctgattta
      361 ccaaaccaaa ttactttttc aaaaaactta gaccaagctg taagtgacgc cgatttagta
      421 atcgaagctg tcccagaaac agttagtata aaagaagatt tttacaaaca attagctaaa
      481 gtggcacctt caaagacaat ctttgcaaca aattcttcaa cacttgttcc aagtcagttt
      541 gctgatataa caggacggcc tgacaaattt ttagcaatgc atttcgctaa caatatttgg
      601 caaaataata ttgttgaaat tatgggacat aagggaactg acgacgaggt tgtcaaagaa
      661 gctttggcat tctcaaaaga tattggtatg gtacctttat atattcataa agaacaacct
      721 ggctatattc ttaattcaat actagttcca ttcttagaga gtgcattagc attgtactat
      781 aataaggttt ctgattctga aactattgat aaaacctgga agttaggaac aggagcaccg
      841 atgggacctt tagaaatttt agatattatt ggcatcgata ccgcttataa tatcatgaag
      901 aattattcag ataccaccag cgatcctaat agtttacatg ctcaccttgc aaaaatgctt
      961 aaagaagaat tcattgataa aggacgtacc ggtaaagctg ctggacacgg cttttatgat
     1021 tacgactaaa ctataaa
//
LOCUS       DATRME010000155         1000 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRME010000155 DATRME010000000
VERSION     DATRME010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(385..603)
                     /locus_tag="VBC23_002091"
                     /pseudo
     CDS             complement(385..603)
                     /locus_tag="VBC23_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          312 a          178 c          134 g          376 t
ORIGIN      
        1 aggcgaggtc taagtaaaga atttaagcgt tttgataata aaaaagtgag aacaatagat
       61 tctcactcac aattagcctg gtaatttctc acacgatttg gtactgcttt ttttaagttg
      121 ggcgatggtg ttatttttaa tatgagtagc tgcactcgaa tccgattgct caaaagcact
      181 tgtcgctcca ttgagatagc tattcagatc agaaataact ggttgaatgt tttggctgac
      241 ttctttgata acttttgatc cagcttctaa gtattttcaa tgttcttgaa cccattggtg
      301 atcttttttt actaagtcac atgctgttaa aatcatcatt aagcttatcc cttccacaat
      361 aataatcaat tttttaaatc ctttttatct tattgatttt ttcaatttta ttgctgtctt
      421 gacgttgcta tcgcaaacaa ccaactcaat gcaatatagt tacggtatac atatcaatgt
      481 aagactcaag atttttaaat tcatattatt tgtaggttct ttatcagacc aagctaatac
      541 tctttcattt gaagtaatat tattatttgt atgatattca agattagcta tttttgattt
      601 cacaaaaaaa ccttcttcta cttttttaga ctgattcttt cttcccactt cttcgtatca
      661 atatcatata tttgaagaac taaactctca gagtttccag tcaattttag taataatccc
      721 aaaactaatc cttcctacaa accaacggat aattttattt caactggtcc tggttttaat
      781 tttacttgtg caatttcgct tgttatacca actattgata tcgccgatac tcctattcct
      841 aatgatgcgc acgcatcatt agttaaacaa agtactttaa atcctacaga gggtcattcc
      901 atcaattaat ggcataggtt ttggcactag aaactgaata tgaattctat tatctattaa
      961 aaaaatcaat atcaccagag tatttataaa acataacgat
//
LOCUS       DATRME010000156          892 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRME010000156 DATRME010000000
VERSION     DATRME010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..298
                     /gene="tilS"
                     /locus_tag="VBC23_002092"
     CDS             <1..298
                     /gene="tilS"
                     /locus_tag="VBC23_002092"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6843986.1"
                     /translation="YGKFISQADITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLF
                     IDEKITLKDREEAVIGEQNKELIFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            303..845
                     /gene="hpt"
                     /locus_tag="VBC23_002093"
     CDS             303..845
                     /gene="hpt"
                     /locus_tag="VBC23_002093"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6843987.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
BASE COUNT          335 a          104 c          172 g          281 t
ORIGIN      
        1 ctatggaaaa ttcatttctc aagcagacat tactattcct atttatgata cttctccgat
       61 tattttaaga agaagaaaag aaggggatag gatattttta ggaaatcaca ctaaaaaaat
      121 tcgccgtcta tttattgatg agaaaataac tctaaaagac agagaagaag ctgttattgg
      181 ggagcaaaat aaagagttga tatttgtgat tgttgcaggt agaacatatt tgagaaaacc
      241 ttctgaacat gatataatga agggcaaact ttatattgaa aatttagaaa aaaggtgaca
      301 tcatgcttga aaatgatata aaaaaagttt tatattcaga agaagatatt attctgaaaa
      361 cgaaagaatt aggagctaaa ttaactgcag attatgcagg taagaatcca ttattagtag
      421 gtgtattaaa ggggtcagtg ccttttatgg ctgaactatt aaagcatatt gatacacatg
      481 ttgaaattga ttttatggtt gtttcaagct atcatggtgg tacaactagt agtggagaag
      541 ttaaaatttt aaaagatgtt gatactaaca ttgaaggaag agatgtaatc tttattgaag
      601 acatcattga tactggacgt acattaaaat accttcgaga tatgtttaag tatcgtcaag
      661 ctaactctgt caaagttgca accctttttg ataagccaga agggcgattg gtagatattg
      721 atgctgatta cgtttgttac gatatcccaa atgaatttat tgtgggattc ggtttagact
      781 atgctgaaaa ctatcgtaac ctaccttatg tcggtgtgtt aaaagaagaa atttactcaa
      841 aatagaaaaa aggtactctc ttaaactatg aaaaataata aaaataatgg tt
//
LOCUS       DATRME010000157          884 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRME010000157 DATRME010000000
VERSION     DATRME010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(214..297)
                     /locus_tag="VBC23_002094"
     tRNA            complement(214..297)
                     /locus_tag="VBC23_002094"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Leu,seq:caa)
     gene            complement(308..379)
                     /locus_tag="VBC23_002095"
     tRNA            complement(308..379)
                     /locus_tag="VBC23_002095"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Gln,seq:ttg)
     gene            complement(386..458)
                     /locus_tag="VBC23_002096"
     tRNA            complement(386..458)
                     /locus_tag="VBC23_002096"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:His,seq:gtg)
     gene            complement(473..543)
                     /locus_tag="VBC23_002097"
     tRNA            complement(473..543)
                     /locus_tag="VBC23_002097"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Trp,seq:cca)
     gene            complement(550..630)
                     /locus_tag="VBC23_002098"
     tRNA            complement(550..630)
                     /locus_tag="VBC23_002098"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Tyr,seq:gta)
     gene            complement(642..714)
                     /locus_tag="VBC23_002099"
     tRNA            complement(642..714)
                     /locus_tag="VBC23_002099"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Phe,seq:gaa)
     gene            complement(717..790)
                     /locus_tag="VBC23_002100"
     tRNA            complement(717..790)
                     /locus_tag="VBC23_002100"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(754..756),aa:Met,seq:cat)
BASE COUNT          249 a          221 c          191 g          223 t
ORIGIN      
        1 ctatgacatt taggagtcct gtttgaattt catttaaatg ttgagtctcc actaactcct
       61 gaaaaatttc cttattattt ctgcttgttt taaaacttca ataacaattg caataatgaa
      121 aaacaaatat aagtaatttt cagtaacttt ttcttaaaat taccagtaca aaacaaaaaa
      181 agacaaggtt tagaaacctt gtcttttaaa gtataccggc ggccggggtc gaaccggcac
      241 gtccgtgagg acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggctat
      301 cttaaaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
      361 accgcttggc tataccccat gaaaaggcga gtgatgggaa tcgaacccac gaatgtcaga
      421 gccacaatct gatgtgttaa ccacttcacc acacccgcca tattagaaaa aacacgggca
      481 gtaggaatcg aacccacact gaaggttttg gagaccttag ttctaccttt aaactatgcc
      541 cgtttactat ggagagagag ggattcgaac ccccgaaccc gaaggagcgg atttacagtc
      601 cgccgcgttt agcctcttcg ctatctctcc taaggtataa atggcgcgag acggaatcga
      661 accgccgaca catggagctt caatccattg ctctaccaac tgagctaccg agcctattgc
      721 gggagcagga tttgaaccta cgaccttcgg gttatgagcc cgacgagcta cctagctgct
      781 ccatcccgcg atatctttaa aggaggatgt gggattcgaa cccacgcacg cttttacacg
      841 cctgacggtt ttcaagaccg ttcccttcag ccggacttgg gtaa
//
LOCUS       DATRME010000158          754 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRME010000158 DATRME010000000
VERSION     DATRME010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            29..343
                     /locus_tag="VBC23_002101"
     CDS             29..343
                     /locus_tag="VBC23_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843988.1"
                     /translation="MFMIMNVTIFIISTLIYSYYYLPKNQGKVWKFNQWNNEKSREQQ
                     YISNFIVTFGLILLVTPFLKYGIYNSFGVLISLLFTATLPSLIVDSLYAAFYVHKHPE
                     YE"
BASE COUNT          274 a           97 c          123 g          260 t
ORIGIN      
        1 tacgttgtat tttggtaagg gtactccaat ttttatgata atgaacgtta cgatatttat
       61 catttctact ttgatctatt cctactatta tttaccaaaa aatcaaggga aagtttggaa
      121 atttaatcaa tggaataacg aaaagagtcg agaacaacaa tacataagta attttattgt
      181 tacatttggt ctgatattgt tagttactcc atttttaaag tatggtatct ataacagttt
      241 tggagtttta atcagtttac tatttacagc aaccttacca tcattaattg ttgattcact
      301 ttatgcggca ttttatgtcc ataagcatcc tgagtatgaa taatcaactt ataaagaaca
      361 tcgaaaacac atgatacctt ttataagaca gaaactataa attcagataa aacatcttcg
      421 cttgataagt cagagacttt aaagatagag gataacaagc tattttagac aaaaaattag
      481 aaatttcaca atagattcta gagtagatac tttattaggt gctggaaggt tattgggcac
      541 ttgattgtga ttggactgga caaaacaaac cagatcaatc agtaactggt tttgcaaaaa
      601 agaattaaaa gatttatttg gagggtaaaa tttttgaaac agagcaatca tagataccat
      661 ggaccgttaa ttgagtttga aatagaatat tcatatatta aaatttttcc atggattgga
      721 ctatttatat cttgcatagg gttatatgca agta
//
LOCUS       DATRME010000159          690 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRME010000159 DATRME010000000
VERSION     DATRME010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBC23_002102"
     CDS             complement(<1..684)
                     /locus_tag="VBC23_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO6843989.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          213 a          130 c           91 g          256 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acataatatt
//
LOCUS       DATRME010000160          680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRME010000160 DATRME010000000
VERSION     DATRME010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBC23_002103"
     tRNA            complement(78..151)
                     /locus_tag="VBC23_002103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBC23_002104"
     tRNA            complement(163..247)
                     /locus_tag="VBC23_002104"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBC23_002105"
     tRNA            complement(256..327)
                     /locus_tag="VBC23_002105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
     gene            complement(361..433)
                     /locus_tag="VBC23_002106"
     tRNA            complement(361..433)
                     /locus_tag="VBC23_002106"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:tgt)
     gene            complement(444..525)
                     /locus_tag="VBC23_002107"
     tRNA            complement(444..525)
                     /locus_tag="VBC23_002107"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Leu,seq:tag)
     gene            complement(535..607)
                     /locus_tag="VBC23_002108"
     tRNA            complement(535..607)
                     /locus_tag="VBC23_002108"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Lys,seq:ttt)
     gene            complement(610..680)
                     /locus_tag="VBC23_002109"
     tRNA            complement(610..680)
                     /locus_tag="VBC23_002109"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asp,seq:gtc)
BASE COUNT          183 a          199 c          150 g          148 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaattgtaa
      361 tgccggctac atgacttgaa cacgcgaccc tctgattaca aatcagatgc tctaccaact
      421 gagctaagcc ggcaatctac taatgcgggt gaagggactt gaacccccac gccgttaagc
      481 gccagatcct aaatctggtg cgtctgccaa ttccgccaca cccgcatttc taaatgaccc
      541 gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc aactgagcta
      601 acgagtctac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg
      661 ataaccgcta cactacggga
//
LOCUS       DATRME010000161          667 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.162, whole genome shotgun sequence.
ACCESSION   DATRME010000161 DATRME010000000
VERSION     DATRME010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.162"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..232
                     /locus_tag="VBC23_002110"
     CDS             <1..232
                     /locus_tag="VBC23_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843990.1"
                     /translation="IGFIDPLTDLGEFDIFQMKFKEPVRYLKNKYSGKPYSPHWQNKI
                     EKMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            293..652
                     /locus_tag="VBC23_002111"
                     /pseudo
     CDS             293..652
                     /locus_tag="VBC23_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT          266 a           80 c          120 g          201 t
ORIGIN      
        1 gataggcttt attgatccac taaccgactt gggggaattt gatatttttc aaatgaagtt
       61 caaagaaccc gttagatact tgaaaaataa atattcagga aagccttatt caccacattg
      121 gcagaataaa attgaaaaaa tgaggtctca tcaagaaagc ctaagagaag aagttaaaga
      181 agagattccg attactagta gggcaagaaa tcaagaaaat aatttgagtt gatcttaaat
      241 tgataaaaag ttcgactttt ttcctaaaaa atagtgtgct ataagtcaat taaaagatgt
      301 gctatattta tttaatagat atcgtctcat tgaatatcaa ctaaccggag aaaattcgaa
      361 tgcaaaaaat ttaatgacag gcactcaaaa tttaaatgct aattttgaag atgaaaaatc
      421 gtcgatggta tattatgaaa atatggtggc tcattatata agactaacaa accatcatgt
      481 gagataccgt gttacactct tatttaaaaa tattgagatg gttgcaagag gggttggtat
      541 ggaaacccaa agtgtggaag ataatcagat atcttttgat atttatattt ttaatgtgca
      601 gtcaggatac aacattaatt atttaacaag atattccgtt aaggaaaatt aaaggagaaa
      661 aatgaat
//
LOCUS       DATRME010000162          657 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.163, whole genome shotgun sequence.
ACCESSION   DATRME010000162 DATRME010000000
VERSION     DATRME010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..638
                     /locus_tag="VBC23_002112"
     CDS             <1..638
                     /locus_tag="VBC23_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6843991.1"
                     /translation="RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLE
                     AKVKNGLIIDFVRSVSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESN
                     HPVLLAFSERWHDSRLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKP
                     FDEQELYRTTQFSSQAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT          223 a          106 c          111 g          217 t
ORIGIN      
        1 gtcgagagtt aaacattgat tttctttttg ctcatcaaga acaagttgtt atattacaag
       61 aaaaatacaa tataaagctt ttgcataact cgtatgattt agagttgctg gcaaattaca
      121 tcatggattt agaagcaaaa gtaaaaaatg gattaatcat agattttgtc cgtagtgtta
      181 gtcctatttt atatcgtcta tttatgattt tacttgcaca agaagtacct cacttgcatg
      241 attacatcta taatgcaaga gatgatcact acgatacttg gaagtttaaa gaattaaagg
      301 agtcaaacca tccagtcctt ttggcattct ctgaaaggtg gcacgatagt cgcttgactt
      361 ctaaaagcct tgcagaatgt ttacaattaa ccgaccttga tgaagaagtg aaatcgacca
      421 tcattcaatt aagacagttc gaaaaatcag tcagaaatcc tttggctcac ctgattaaac
      481 cttttgatga gcaagaacta tatcgtacaa ctcaattttc ttctcaagca tttttagacc
      541 agattatctt cttggcaaag gtaattggtg ttgagtatga tactgttaat tttcactacg
      601 atacggttaa caagcttatt ataaagatac ttgagtaatt taaaaacagt tctgaga
//
LOCUS       DATRME010000163          614 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRME010000163 DATRME010000000
VERSION     DATRME010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(12..>614)
                     /locus_tag="VBC23_002113"
     CDS             complement(12..>614)
                     /locus_tag="VBC23_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
                     /protein_id="HEO6843992.1"
                     /translation="MKSMGLTARIRRKRKYASYKGEVGKKADNLIQRQFEGSKPYEKC
                     YTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPALKQVQTMLERAFPAASYSETI
                     LHSDQGWQYQHKSYHQFLEDKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLEKSY
                     KSLDDLEQAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFT"
BASE COUNT          169 a          136 c          131 g          178 t
ORIGIN      
        1 gacaatttat tttaagtgaa ggatttagtt ctgtattgca caggactaag tccttttagt
       61 tttgctttga ttcgcttgtt gttgtagtaa aaaatgtaat ctgtgatagc ttgctcaagg
      121 tcatcaagtg atttgtaaga cttctccaag ccatagaaca tctcagactt tagaatccca
      181 aagaaagatt ccatcatacc gttgtcagga ctgttcccct tccgtgacat agaaggacga
      241 atgcctttgt cctccagaaa ttgatggtag gacttgtgct ggtactgcca gccttggtcg
      301 ctgtggagaa tggtttcgct gtatgaagcc gcaggaaaag ccctctcaag catggtttga
      361 acctgtttca aggcaggaga ccgagacagg gtaaaatcaa ttatctcact gttatagcca
      421 tcaagaacag gcgatagata aagtttccct tcaggtaaag caaactccgt cacatcggta
      481 tagcacttct catagggctt cgacccctca aactggcgtt gaatcaggtt atcagccttc
      541 ttaccaacct cacctttgta agaggcatac ttgcgcttac gacgaatacg agcagtcaag
      601 cccatacttt tcat
//
LOCUS       DATRME010000164          590 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRME010000164 DATRME010000000
VERSION     DATRME010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(<1..312)
                     /locus_tag="VBC23_002114"
     CDS             complement(<1..312)
                     /locus_tag="VBC23_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEO6843993.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TL"
BASE COUNT          204 a           97 c           86 g          203 t
ORIGIN      
        1 gagtgtctca ggatagttct gatataaata ggataaaaca gctgcaactg tatcattaga
       61 taaataagtt tcttttagta atgtgccatc ttggtcaatg acacgaccac cattttcagc
      121 gacgtaggcg atacgattag ggtattccaa aaagtttaac aacaattgat cgtataaatt
      181 accactagca gctacaaatt taatatcata cttctccatc aacgttaata aagaagaaaa
      241 ccgtgcttta tcataagttt tatctgagcg tagaaaagtt ccatccatat ctgttgctat
      301 tagtttaatc atacaagtaa cctttgcctt tcaagagggt gccaaatatc tcaatatgtt
      361 ctttcattat atcacatata aaccaaaaag atttgatacc tgatacgtca acggttctat
      421 taattaagtt aaattttgtt tcagagatta aatcgacttt tacctatact tatctccata
      481 ctatcagagc ttgtttaaca taatgattta ctgaagagtt ttcttattgt tgagttgaat
      541 aatagattcg taactattac aatagtcata ataaaatatt tgagattcga
//
LOCUS       DATRME010000165          577 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.168, whole genome shotgun sequence.
ACCESSION   DATRME010000165 DATRME010000000
VERSION     DATRME010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.168"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
BASE COUNT          191 a          119 c           61 g          206 t
ORIGIN      
        1 taatttcatg agatttaatt tccctttttg tgtattattt ctattctaca atagtcttac
       61 ccttagaaat atttttccat ttttcaatga tacgagctac tctttcttac tcctcgtcat
      121 ggtacctccc aaaattactt catcaatatc ttctcattga aataatatac ttgttctaaa
      181 ttgacctctc tggaaaaatc ctgccagata atcaaaatat aatcttgatc aataatattt
      241 gctcatgaaa cggctccaca aaatttctta catctttaac attttcatag agtgctttta
      301 ctccacaatg gccaaagtac ctataacaac tgctactact ggtacaaatt ggagttgtta
      361 tgattactac aaaaacactc tcagcaagcg tagagactat tctacccact tcattttctc
      421 tagatattta ttgctcctac cttaaattaa ttaatttatc actcatattc gggatattta
      481 tgaatataat atgctgcata aagggaatcg acaatcagag ctgtaaaagt tgccgtaaat
      541 aatacacctc caatcaaacc aaaaatattt tcaatac
//
LOCUS       DATRME010000166          516 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.169, whole genome shotgun sequence.
ACCESSION   DATRME010000166 DATRME010000000
VERSION     DATRME010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..>516
                     /locus_tag="VBC23_002115"
     CDS             <1..>516
                     /locus_tag="VBC23_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO6843994.1"
                     /translation="EKLLVESREGIPLNKETFWKIDRVLSNGVKKGQRIYHILKTNDL
                     EVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIKEGRRYEDFIEHMN
                     QSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIETAKHIQVIKRTLY
                     DNKRDFFELFPV"
BASE COUNT          172 a          115 c          102 g          127 t
ORIGIN      
        1 gaaaaacttt tagttgaatc tagagaagga atccctttga acaaagagac cttctggaaa
       61 atagatagag tgctttctaa tggggtcaag aagggccaac gtatctatca tatcctcaaa
      121 accaacgatc tagaagtgag ttcttcaacc gtttatcgac acatcaagaa aggctaccta
      181 tccatcacac caatcgacct acccagagct gtgaagttca aaaaaaggcg aaagagcaca
      241 ctccctccta ttccaaaagc gattaaagaa gggcgacggt acgaggattt tatagaacac
      301 atgaaccaat cagagctgaa ctcctggctt gaaatggaca cggttattgg acggattggt
      361 ggaaaggtcc ttctcacctt caatgtcgcc ttctgtaact tcatctttgc caaactgatg
      421 gattctaaga cagctatcga aactgctaaa cacatacagg tcattaagag gacactctat
      481 gataacaaga gagacttctt cgaactcttt cctgtt
//
LOCUS       DATRME010000167          508 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.171, whole genome shotgun sequence.
ACCESSION   DATRME010000167 DATRME010000000
VERSION     DATRME010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.171"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..229
                     /locus_tag="VBC23_002116"
     CDS             <1..229
                     /locus_tag="VBC23_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6843995.1"
                     /translation="SRKATINPEDINSPIKGFPKTVITCFARETFNRILEELPHREIA
                     RTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            226..>508
                     /locus_tag="VBC23_002117"
     CDS             226..>508
                     /locus_tag="VBC23_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6843996.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIP"
BASE COUNT          171 a           72 c          100 g          165 t
ORIGIN      
        1 tagtagaaaa gcgactatta atcctgaaga tattaattca ccaataaaag gttttccaaa
       61 gactgttatt acctgttttg cgcgggagac tttcaatcgc attttagaag aattgcctca
      121 tcgtgaaatt gctagaacta gtgtagcaaa cttagaaatt cccatttatg aattagaatt
      181 taaaggacaa aagattgtcc ttatcaagag gttggtgatg aagatttgat ttgtttgcat
      241 gggatgaatt gggtagatgg tcaattggca gcttattata gattgatacc agaagatgat
      301 aaggtgcatt tgggacgtgt gattgttaat ccagacttta gaaaaaaggg attaggaaat
      361 caattagtgg aatatgagat taaatttagc gaggcaaatt accctaataa acctatttat
      421 gctcaggccc aagcttattt acaagatttt tatcaatctt tcggatttca acctatctca
      481 gatatctatc tagaagataa tattcctc
//
LOCUS       DATRME010000168          504 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRME010000168 DATRME010000000
VERSION     DATRME010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..>504
                     /locus_tag="VBC23_002118"
     CDS             <1..>504
                     /locus_tag="VBC23_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6843997.1"
                     /translation="ETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNIIQ
                     DGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLRF
                     VRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFWK
                     SPKGWKCR"
BASE COUNT          143 a           99 c          124 g          138 t
ORIGIN      
        1 gaaacagcca ttgttcagct acttgagtat ttaaacgatg gctacgagtg gattgtggac
       61 attgacttgg aaaagttctt cgatactgtt ccgcaagaca gattgatgtc cctggttcat
      121 aatatcattc aagatggcga tacggagtca ctgattcgta agtacttcca ttcgggagtt
      181 gttattaacg gacagcgaca taagacttta gtcgggacac ctcaaggcgg gaatctatca
      241 cccctcctat ctaatattat gcttaatgag ttagacaaag ggttggaaaa gcgaggtctt
      301 cgctttgtcc gttacgccga tgactgtgtc atcactgtcg gaagcgaagc agctgctaag
      361 cgggtcatgc attcggtcag tagctatatt gagaagcgat tagggttgaa agtcaacatg
      421 actaagacca agattgtcag accgaacaaa ctcaaatacc tcggatttgg tttctggaaa
      481 tctccaaaag gttggaagtg tcgt
//
LOCUS       DATRME010000169          501 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.173, whole genome shotgun sequence.
ACCESSION   DATRME010000169 DATRME010000000
VERSION     DATRME010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.173"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            complement(27..485)
                     /locus_tag="VBC23_002119"
     CDS             complement(27..485)
                     /locus_tag="VBC23_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6843998.1"
                     /translation="MILAGVTLENVKSNQECPKLWEKLHKQYNPSKLKEKGIQQAIGI
                     CKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMPSSLQETWRKII
                     QGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKTNLI"
BASE COUNT          134 a           76 c           95 g          196 t
ORIGIN      
        1 aaggggcttt tttatattaa gcaatactag attaagtttg ttttcgagtt attagtaata
       61 tctgcgatag caagccaaat ttccatttga taatcagtat cttgtggatg ttgagatgaa
      121 taaatttcta agtttggcga gttagctggt tttagattat tctcttgaaa aaatccttgt
      181 attatttttc gccatgtttc ttgaagactc gatggcattg gacctttaac agaaatgact
      241 gcataggtag cgcttgggat cctaataatt tctaaccctt ttggggcttg gacacttgtc
      301 tctacttgat aagtggcact gtaatcgaaa cgaaaatcag gttgtgtttt acaaatgcca
      361 atggcttgct ggataccttt ttcttttagt tttgatggat tatattgttt atgtaatttt
      421 tcccaaagtt taggacattc ttgatttgac tttacatttt ctaaagttac tccagcaagt
      481 atcattgctg gcttttcaac a
//
LOCUS       DATRME010000170          490 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRME010000170 DATRME010000000
VERSION     DATRME010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            13..>490
                     /locus_tag="VBC23_002120"
     CDS             13..>490
                     /locus_tag="VBC23_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6843999.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYD"
BASE COUNT          180 a           77 c           91 g          142 t
ORIGIN      
        1 aaaaaatatt taatatcact tgaagattac tatcttttat ctaagaaatt ttcccaagta
       61 atgacgctcg attcgcatag ttcagacgac ggctatacta tccgttcgct atactttgat
      121 tctttagaag atgttgattg gcaagaaaag gaagatggtg ttgagatacg aagaaagatt
      181 cgtttgcgca attatggtag tcaaagcaat tttgcgaaat tagaaatgaa acaaaaacaa
      241 ggtgctaatc aaaaaaagcg ctctctaaaa ttaagcaaga cagatgctca agcgctgatt
      301 aatggtaaca atagtgtttt attaaattat ccggatacat ttgctgcgga gtgttattca
      361 aggataacaa cgcaacttta tcaacctaaa acggttattg agtaccaaag aaaagctttt
      421 gttgctaagg aaaacaatat ccgaatcacc tttgaccacc atataatagg tacagaggct
      481 agttatgata
//
LOCUS       DATRME010000171          437 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.177, whole genome shotgun sequence.
ACCESSION   DATRME010000171 DATRME010000000
VERSION     DATRME010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.177"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            34..>437
                     /locus_tag="VBC23_002121"
     CDS             34..>437
                     /locus_tag="VBC23_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6844000.1"
                     /translation="MKLSYEDKLEIYELRKIGMSWSQISQRYDVRISNLKYMIKLMDR
                     YGVEIVEKGRNEYYPPELKQEMIDKVLIHGCSQLSVSLDYALSNCSILTNWLSQFKKN
                     GYTIVEKTRGRPSKMGRKRKKTWEEMTELERL"
BASE COUNT          154 a           63 c           93 g          127 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttca
//
LOCUS       DATRME010000172          425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.179, whole genome shotgun sequence.
ACCESSION   DATRME010000172 DATRME010000000
VERSION     DATRME010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.179"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..380
                     /locus_tag="VBC23_002122"
     CDS             <1..380
                     /locus_tag="VBC23_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6844001.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          129 a           79 c          116 g          101 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat cttgaacgac
      361 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
      421 ggcta
//
LOCUS       DATRME010000173          397 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.180, whole genome shotgun sequence.
ACCESSION   DATRME010000173 DATRME010000000
VERSION     DATRME010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            90..>397
                     /locus_tag="VBC23_002123"
     CDS             90..>397
                     /locus_tag="VBC23_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6844002.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     "
BASE COUNT          143 a           79 c           90 g           85 t
ORIGIN      
        1 tagaactaga tgcggttacg tattgactag aggaaggttc accaatgtaa gatatcatgc
       61 agacaacgaa aaaagaaagg aatacgcaca tgtcagaatt gctagataag atatcatctc
      121 ggaacaatat gctcgaagct tacaagcaag tgaaatcaaa caaaggttct gctggtatcg
      181 atggggtcac tatcgagcag atggatgact atcttcacca aaattggcga gaaaccaaga
      241 aactcatcaa agagaggagc tataaacctc aaccggttct cagggttgaa atcccaaaac
      301 caaacggagg agttcgtaac ctaggtatcc cgacggctat ggatagaatg attcagcagg
      361 ccatcgttca agttttgagt ccactctgcg aaaaaca
//
LOCUS       DATRME010000174          397 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.181, whole genome shotgun sequence.
ACCESSION   DATRME010000174 DATRME010000000
VERSION     DATRME010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
BASE COUNT          149 a           64 c           53 g          131 t
ORIGIN      
        1 tttcgtatct atttattcaa aggaaaatct caaataactc attatgaaga atgcgatacg
       61 aaatctaatt ctcatgttta tatagtttta ttatcaaaaa ctacctaatg ctctattgaa
      121 aaaactagac ttagggtagc gatgataaac aatgaactga tgtctggtta aggaaatagt
      181 tattaagcaa tacagatttt atgatgcata ctggctgttt caataatcat cccgtacttg
      241 acagtaccta tctaatttta cataagttca cctcccaatt tagcttacaa taaatagaca
      301 tttaactctt aaagcgaaga attacgttta ctagtaaaat tatcaatcta taagttacta
      361 gacaccagtg acaaaatagt gttgcctaaa tactaga
//
LOCUS       DATRME010000175          363 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.183, whole genome shotgun sequence.
ACCESSION   DATRME010000175 DATRME010000000
VERSION     DATRME010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.183"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..>363
                     /locus_tag="VBC23_002124"
     CDS             <1..>363
                     /locus_tag="VBC23_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6844003.1"
                     /translation="AKEFSERDCRVIYIEKSNGGLSEARNYGIYHSKGKYLTFVDSDD
                     KVSSDYIANLYNAIQKHDSSIAIGGYLEFYERHNSIRNYEYLDKVISVEEALLNMYDI
                     KTYGSIFITAWGKLFHKS"
BASE COUNT          135 a           42 c           64 g          122 t
ORIGIN      
        1 tgccaaagaa ttttctgaaa gagattgtag agttatctat atagaaaagt cgaatggtgg
       61 gttgtcagaa gctagaaact atggaattta tcattcaaag ggaaaatact taacttttgt
      121 tgattcagat gataaagttt cctctgatta catagcgaat ttgtataatg ctattcaaaa
      181 acatgattcg tctatagcta tcggtggcta tttagaattt tatgaaagac ataatagcat
      241 aagaaattat gaatatttag acaaagtgat atcagttgaa gaagcactac taaacatgta
      301 tgacattaaa acttatggtt caatttttat tactgcatgg ggaaaattat tccataaatc
      361 tat
//
LOCUS       DATRME010000176          344 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.denovo.184, whole genome shotgun sequence.
ACCESSION   DATRME010000176 DATRME010000000
VERSION     DATRME010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.denovo.184"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..>344
                     /locus_tag="VBC23_002125"
     CDS             <1..>344
                     /locus_tag="VBC23_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6844004.1"
                     /translation="IVFTTHSLSLLEYINDTSNDKVTINYLTKHTGQINVVENPKNDY
                     LRRDLMLKLGQPIKQSISVLTEDDTARWYLAHLLNYYVQKRPNTKLQIDTLKMVDCHI
                     GWSSIVSLVKND"
BASE COUNT          132 a           46 c           53 g          113 t
ORIGIN      
        1 aaatagtttt cacaacacat agcttgtcat tattagaata tattaatgat acttcaaatg
       61 acaaagttac tattaattat ttaacaaaac atactggtca aattaatgtt gtagaaaatc
      121 ctaaaaatga ttatttaaga cgtgatttaa tgttaaaact cggtcagccg attaagcaat
      181 ccatttcagt tttaacggaa gatgatacgg caaggtggta tctagctcat ttacttaatt
      241 attacgttca gaagcgacca aatacaaaac ttcaaattga cacattaaaa atggttgatt
      301 gtcatattgg atggagcagt attgtaagtc ttgtaaaaaa tgat
//
LOCUS       DATRME010000177         7865 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699731-rid18553533.guided.087, whole genome shotgun sequence.
ACCESSION   DATRME010000177 DATRME010000000
VERSION     DATRME010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699731
            Sequence Read Archive: SRR7789295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790155.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790155.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,093
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 44 of 67
            Pseudo Genes (incomplete)         :: 12 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699731-rid18553533.guided.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-06-05"
                     /collected_by="RMPRU"
     gene            <1..279
                     /locus_tag="VBC23_002126"
     CDS             <1..279
                     /locus_tag="VBC23_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6844005.1"
                     /translation="VFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVIAIQEIFGK
                     SQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(497..1690)
                     /locus_tag="VBC23_002127"
     CDS             complement(497..1690)
                     /locus_tag="VBC23_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6844006.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1769..1975)
                     /locus_tag="VBC23_002128"
     CDS             complement(1769..1975)
                     /locus_tag="VBC23_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6844007.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(1968..3110)
                     /locus_tag="VBC23_002129"
     CDS             complement(1968..3110)
                     /locus_tag="VBC23_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6844008.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            3622..4257
                     /locus_tag="VBC23_002130"
     CDS             3622..4257
                     /locus_tag="VBC23_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6844009.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4411..4488
                     /locus_tag="VBC23_002131"
                     /pseudo
     CDS             4411..4488
                     /locus_tag="VBC23_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4733..4849
                     /locus_tag="VBC23_002132"
     CDS             4733..4849
                     /locus_tag="VBC23_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6844010.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            4865..6784
                     /gene="tet(M)"
                     /locus_tag="VBC23_002133"
     CDS             4865..6784
                     /gene="tet(M)"
                     /locus_tag="VBC23_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6844011.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            6903..7070
                     /locus_tag="VBC23_002134"
     CDS             6903..7070
                     /locus_tag="VBC23_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6844012.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7130..7483)
                     /locus_tag="VBC23_002135"
     CDS             complement(7130..7483)
                     /locus_tag="VBC23_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6844013.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         2378 a         1422 c         1506 g         2559 t
ORIGIN      
        1 gttttcatta ttattatttt tccacaagct tttcgcaaaa tgttgccagc cattatttct
       61 cagtttgtaa cggttataaa agatacatca ctactttatt ctgttatagc tattcaagaa
      121 atcttcggaa aaagtcaaat tctaatggga agatatttcg aagctggaca agtttttaca
      181 ctctacgcta ttataactgc tgtctatttc attacaaact ttatcatctc aagtttttca
      241 cgtaaacttt caaaacgttg ggagcaagct actgagtaat taaaacgaag gccttttaga
      301 ccttcgtttt tttatatctt atttaaatta aattatatat gcctttttga ctatcttttt
      361 atatcttctc aaatgtccgc aaagccttat tttatcggct ctacgggcat tttgcttttg
      421 tggtaaacct cacatatcta ggtctatctt tttatatttt cgctatcaaa cgtggttaaa
      481 atcgtggtaa atacttctat gcgattagac gctgaacctc tgattttgcg gtatctatgg
      541 acgcatgagc ataccagttc attgtgatac tgatgtttga atgtcccatg atatactgta
      601 aatctttcgg gttcatgttc ttgcttgcca gccttgtgca gaacgtatgg cgtagcgtat
      661 gcggtgtgat atgtggcaag ggattgtcct tgtgttgctt gttgtatttc tttaccatac
      721 ggacaaataa ggcattgtaa tcaatcgcaa ctttgggctt gcctttatga ttgacaaaca
      781 gaaaattgct ccgtccgtct atcacaaatg gttctgcctt tgggcgtttt ttcataaccc
      841 gttgaaatgc ctgtattgtt tctctgctta atggcacttg ccttgttccg ttctttgttt
      901 taggcgtttc aatataatag ccctgttcct tgctctttaa taactggtgg tcgataatca
      961 caacttcatt cttgaaatca atatcggcta ctgtcagtcc gcacagttcc gagatacgaa
     1021 gtccagtctt taacagtatc agcacatcat cataatactt atgatacacg ttgtccgtct
     1081 taataaatga cagtagggct tgttcttgtt cctctgtcaa tgccactttc tctttggtat
     1141 cattttctag gacttcactt aacttgaaat caaaagggtt cttccttaca caatcgtctt
     1201 gtatggcgat atagaatgac gcttttaacg agcgtttatg gttgttaata gtattgtagg
     1261 aaaagccttt ctctttcatg cgtaacgccc attccttagc gtcagagggt tttatcgtat
     1321 caatgctcct agcacccaac ttgtcctctt tcaataaccg catgagctgt tcccgttgtt
     1381 tcattgtgct tttcttcacg tttgccctct gttcgttctg tttggcgtag agttggcaaa
     1441 gcgtcatttt cttgcctgtg ctgtcgatac cgtcctcaat atcccgtctt atctgctgtt
     1501 ccagttcacg aagtgaggtt ttttcccgtt ttccctttgg tgtcgggtct gtgggtgtca
     1561 atctccaagc atacacatat tttgtatttc caaatgcgtc tacatattta tacaagtatt
     1621 ttccgtctgt tcgttggctc tctccagtat gcaggatacg tcctttgtta tcccgtcttt
     1681 tttctttcat ggtgtctgct cctttccttg ttggaaagag ccttgatatg acttgtgttc
     1741 atcataacac atacaaggct catttgcatt agattgcgtc caatttatca ataacctgtt
     1801 caaactgtcg tcgtttaatc tgtatgcggt tgccattcat aatgagccag ccagcgtcct
     1861 tgttttcctc tgccaagcgt cttaacttgt tttctccgat acggaaatac tttgacgctt
     1921 cttcaatggt aagggtgtat ttttcccata cgggaatatc ctgtgcatta ctcataaaac
     1981 accccctcac tttcaattac gtctgctatg tccgtacaca tttgttccac aagcatttcc
     2041 tgtttttcag aaaggtgggt acattcatca atcattttca tgtagtttga gcctgtcagc
     2101 ctgtcgattt cctctatcat cacgattgac ggcatgacct gtctttccag ccattgcaga
     2161 gtgcgttcca tattgacagg tacggggttc atttcaaaag gtaaactatc atgggaaatg
     2221 aatatctccc acagcggata gtcgggaaat tgcacaaaat cagtaataag gtaaaacacc
     2281 agcccatgag ggttatacgt caataacagt tcctcaactg cctgtactgc ctttttgtca
     2341 cgcagacgaa tttcaaaacg gttgataata tccgtatgtt tctttttagt tgcctgttcc
     2401 ttttgttttt catacaggca gaaatatttt gtgcttgctt ttgagccgat ataaagggtg
     2461 cttgctaaat tgcggttttt tccgcttagt ttaccgcctt gcacattttc atagttttta
     2521 cagcgacaat ccgcaccgcc atttttgtat ttttcagata aaacgggaat atccagcaga
     2581 ccgcacatat cgttgattgc caagtcaaaa cgtcggataa caccgccaca gtcaagacag
     2641 cggttcaaga atgaatacca atccctgttc tgtgcctgta aaacatattc catattgcgt
     2701 gagcctgccc cttttaattc caaaaatacg cccatgtgtt catcatcaga agccataacc
     2761 ttgatttctc cgtaggcgta atgctcttta tagccataat atccatagtc ataatggata
     2821 aaataatctg ctttcatgcc aagcaccttt cggataattt ccaatgcgtc agtcgttgga
     2881 aaacggacag acagataatc aacgagcaga taaaaaggct tatcgcagat atagtaattc
     2941 agacaacgca ggataatatt ttgtgtttcc ttgctggcgt tggcttttcc gttctcaaag
     3001 cggttgatac tatatcttga tacatgggta agacttgcaa gttctccttg tgaaagaccg
     3061 cttgcaagac gtttattctt catttcaagt gcaaattcta aattgttcat tgtattcctc
     3121 caatcccaca atagccttga aatacctgta aaccactatt caaaagaatg tgaaattact
     3181 atcttcttaa aactcctcat tactccttac agctttgggg ttgtatggtt tttgacttgt
     3241 cttttgtgaa atagtgcccc cctgttagat aacgggggca cagacaaggc tcgcaggctc
     3301 gccagccgac aaagcatttc ttccccttga cacaaggtgt catggcggta agcctgagag
     3361 gcttccgcca ccgtgtcaga aatactttgt cagcaagttc tgttttgcct tttctaacaa
     3421 gttccatttc aaccgatatt catatctacg gatttaatca gataggtatt tctttttgaa
     3481 tgtttttata tttcattcta ttgtgcttat tcgattacaa gaacatagta attcttatat
     3541 gcttatttgt caatatcttt ttttgccttt acaaatcata ttaagcatga tagacttata
     3601 atagcgaaga tataacgaga tatggagggt tatgcaatga aaataagcca tttaggaaat
     3661 aatatacaga ccataagaaa atttagggga atgaaacaac aggaacttgc ggacaaaatc
     3721 ggtatcaata tgcagagcct ttccaagatt gaaagagggt tgaactatcc tgcctatgaa
     3781 acgctggaaa agataatgga agtgctggac gttacgccca atgaattgtt gtcgggagaa
     3841 tggaagtatg tcaatcaatc tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt
     3901 ctaaatgcag aactgaaaca tggacactat gataacttct ttgacagcga ggaagaatgg
     3961 ctggaatatg agttagaaaa gttacgggaa tatattacag actatatcaa cgggaaaagc
     4021 attgaagcgt ccgaccttta cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa
     4081 ttattagacc gctatgatga tttatacagt atggatatgt ttggggaaag catagaggga
     4141 cataagtata agacacctta tcaagtcgtg aaaatgataa acccgaactc aaaagaagat
     4201 atggaactgt tacgagaagt attaaggaat aacaattttg atgatgagga cgagtaactt
     4261 ttttcctctt gcgagaagat acagcaaaca aaaagcccga tcaagagtgc aaagcaccac
     4321 caatggtggc aaagctcttg acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa
     4381 gaaagggata aaatctgatt tgtggtaaat caggcaaagg caacgcaggt atctcaattt
     4441 gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca aatattggta
     4501 cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg agattccttt
     4561 acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg
     4621 cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc
     4681 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
     4741 aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag
     4801 tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa tggaggaaaa
     4861 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa aaactacctt
     4921 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
     4981 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
     5041 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
     5101 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
     5161 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
     5221 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
     5281 ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga
     5341 actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat
     5401 agagggaaac gatgaccttt tagagaaata tacgtctggg aaattattgg aagcattaga
     5461 actcgaacaa gaggaaagca taagatttca taattgttcc ctgttccctg tttatcacgg
     5521 aagtgcaaaa aacaatatag ggattgataa ccttatagaa gtgattacga ataaatttta
     5581 ttcatcaaca catcgaggtc agtctgaact ttgcggaaaa gttttcaaaa ttgagtattc
     5641 ggaaaaaaga cagcgtcttg catatatacg tctttatagt ggcgtactgc atttgcgaga
     5701 ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata cttcaataaa
     5761 tggtgaatta tgtaaaatcg ataaggctta ttccggggaa attgttattt tgcaaaatga
     5821 gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga gaaaaaagat
     5881 tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg aacagagaga
     5941 aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac gatattacgt
     6001 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat
     6061 tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ataaaagagc ctacagtcat
     6121 ttatatggaa agaccgttaa aaaaagcaga gtataccatt cacatcgaag ttccaccgaa
     6181 tcctttctgg gcttccattg gtctatctgt agcacagctt ccattaggga gcggagtaca
     6241 gtatgagagc tcggtttctc ttggatactt aaatcaatcg tttcaaaatg cagttatgga
     6301 ggggatacgc tatggctgtg aacaaggatt gtatggttgg aatgtgacgg actgtaaaat
     6361 ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct
     6421 tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata
     6481 tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc
     6541 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
     6601 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg
     6661 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
     6721 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
     6781 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
     6841 ttaataaaga tttgaattaa agtgtaaagg aggagatagt tattataaac tacaagtgga
     6901 tattgtgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa
     6961 aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt
     7021 tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta
     7081 atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga
     7141 tgtttcttgc ttcgttgata ccgctggcta aagattccat taaggatagt tctttgtctg
     7201 taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag
     7261 caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt
     7321 gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa
     7381 tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta
     7441 aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac cacctctata
     7501 cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata
     7561 ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt
     7621 gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa
     7681 cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa
     7741 agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat
     7801 agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc
     7861 agaac
//
